LOCUS       DAOOMU010000001        63942 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_293_length_63942_cov_22.443611, whole genome shotgun sequence.
ACCESSION   DAOOMU010000001 DAOOMU010000000
VERSION     DAOOMU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 63942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 63942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63942
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_293_length_63942_cov_22.443611"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1160
                     /locus_tag="PLI55_00005"
     CDS             39..1160
                     /locus_tag="PLI55_00005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPA99252.1"
                     /translation="MAVNIVEVATPRQLKQFIKFPLKLYRNNPYYIPPLNFDERNTLD
                     PRRNPACEFCDTRYWLAYKNGKLAGRIAGIYNKRFVEICGKKYLRFGWVDFIDDEEVV
                     VALFKALEDWGKELGVEAIHGPLGFTDLDREGMLIEGFQEIGTMATIYNYPYYPKYLE
                     QLGYQKDIDWVEYDITVPKVMPQKLVNVSQIVKQRLGLHTLQIKKIKDVLPYAHQIFE
                     VLNATYAVLYGFVPLTEKQIDTYVKQYISLMNPDFIAIVLDKNDQIVGIGLTMPSLAR
                     ALQKARGKLFPFGFVHLLWALRHAKVADLLIVGVLPEYQGQGVPALMIAEIGMKLIRK
                     GIQRAESNPELETNIKVQSQWKYFDLRQHKRRRCYIKHV"
     gene            1191..2042
                     /locus_tag="PLI55_00010"
     CDS             1191..2042
                     /locus_tag="PLI55_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007359682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HPA99253.1"
                     /translation="MFAKKTDIGNQPVRSLQNVRHIILDLDGTIYNGHTLFPFIKDFL
                     DDLKNLQVDYTFLTNNSSLSVTAYIRKLRRMGLPVKPTQVYTSAQATIDYLRRRHPAI
                     KRIYLVGTQSLRREFIDSGFTVVGDDPTNEPDAVIVGFDTSLTFQKLCGAAYWIKTGK
                     LFIATHPDLTCPTDQDTVLVDCGAVCAALKAATGKKPSAVLGKPDPSMIKGIMQRFGL
                     KKDEVAIVGDRLYTDIKMARQAGIASVLVLSGETTQADLQESPILPDYVLENVGELSK
                     ILRQSRK"
     gene            complement(2187..2263)
                     /locus_tag="PLI55_00015"
     tRNA            complement(2187..2263)
                     /locus_tag="PLI55_00015"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2227..2229),aa:Asp,seq:gtc)
     assembly_gap    2330..2429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2507..3610
                     /gene="ychF"
                     /locus_tag="PLI55_00020"
     CDS             2507..3610
                     /gene="ychF"
                     /locus_tag="PLI55_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPA99254.1"
                     /translation="MGFKCGIIGLPNVGKSTLFNALTHAGVAMENYPFCTINPHIGVV
                     NVPDKRLDLLAQIYKPDKIIPTTLQFVDIAGLVRGASQGEGLGNQFLSHIQAVDAIIH
                     VVRCFENPNVAHIDEKLDPQRDIEIVETEILLKDLEIVENRREKLIARAKSGDEQTQK
                     QLKILDDIRGLLIKGQSAKQYHSHPEEEYFIRDLALLSSKPVIILGNISEDEAISGNK
                     SAITEAFEKFALQTGNLFLTVSANLELELSELPPDDRKLLMNEWKITEPGLDRLIRAG
                     YELLRLITFFTMESKIVQAWTIPVNTPAVKAAAEIHTSFEDRFIKAEVRHWKDIESLR
                     SENLLREQGLVHIEGKSYLVHDGDVITFKLAAG"
     gene            3579..4307
                     /locus_tag="PLI55_00025"
     CDS             3579..4307
                     /locus_tag="PLI55_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA99255.1"
                     /translation="MSLPLNSPPANMASLVSQFDKLNSQYNLIADNQKILIAVSGGID
                     SLVLSRLLFELRLAEFKNLELLAAYVRQKSVALINEALKEIADLLADWQIPFSILDSE
                     LPESSQINCYACARERRKRLFQFAIMNDCNAIAFGHNLDDYLETGLMNLVFKGNLESL
                     QPVQMQLGGRIRIVRPLLNWPKKHIRASGQAYGIKSIAPVCPMASFNKRQDINAMITS
                     FSRLNRGFRSNLRRAINHWNQLSI"
     gene            complement(4443..5036)
                     /gene="nadD"
                     /locus_tag="PLI55_00030"
     CDS             complement(4443..5036)
                     /gene="nadD"
                     /locus_tag="PLI55_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPA99256.1"
                     /translation="MKVGLFGGTFDPPHNGHLEIARAAIRAFSLNKLIFIPANISPTK
                     TSEIHTSPVHRLMMLKLALQNEPACEISTIEIERGGISYSIDTIRAIKHQMRLEREDL
                     FFVVGADSLIEFHKWRQPEDILQEVQVVVARRPGCDLSLVKPEYLAKVLFFNAPLIPF
                     SSSDIRQIAMNGGQIDQFVPPAVAEYIRQNELYRRSA"
     gene            complement(5033..5833)
                     /gene="bamD"
                     /locus_tag="PLI55_00035"
     CDS             complement(5033..5833)
                     /gene="bamD"
                     /locus_tag="PLI55_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPA99257.1"
                     /translation="MPPKRQILKIVLISLLIIYLNNCASNKPKPVVDFEQHYRQGTEY
                     FNKKKWDKAIEHFTLVVINSPGSELADDAQFYIAECYFKRKEYLLAASEYQQLINRYA
                     YSEYVEEAFYKIALSQYKSSPRYQLDQEYSIYALQSFQDFIDTYPQSRFRADSEQKIA
                     EIRSKLARKLFESGKLYRKMQEWKAAIIYFDKVLETYYDTEWAEKARLEKAYCLIRQR
                     QFDQYELLFKEILARSTPQTDPNSLEYLRTSYHKELRKIEREKRKRAD"
     gene            complement(5878..6780)
                     /locus_tag="PLI55_00040"
     CDS             complement(5878..6780)
                     /locus_tag="PLI55_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99258.1"
                     /translation="MKSKFLAATLLCAILIPIMGFGQSFFDERTTVTERDRLIYSMKY
                     AQADEDTFVFNYEKSPGLAFFLSAVVPGAGEFYAGAKYRAAAFFSVEVVSWVGYFNRK
                     NNGEKLERKYMNWANTYWDAWQWYENSLSYPEYFGLSDSGSGFGSHTIYFEYEGKEYK
                     ADVSYLDRELPGWQTYLQEGTLRPIKTRDYYENIGKYDQFASGWSDFDQIVSEVDSLN
                     IPTSPLRKQYLNQRYESNQSLKMATNFTTVIMFNHLISAFHAQIAAKNYRPPEQKDVS
                     WHVGLVTDCTYKMPIRGLSLSVAF"
     gene            complement(6791..9832)
                     /locus_tag="PLI55_00045"
     CDS             complement(6791..9832)
                     /locus_tag="PLI55_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99259.1"
                     /translation="MSERQICRNTLVIFLIMLFFFNPAGAQFGTYNHPELEWLSFETA
                     HFKIHYHQGAEWTARRTAEVAETIYGPITEFYQFEPSEKTALVIRDTDDISNGASYYY
                     DNKIEIWATPLDFPFRGNHDWLRDVITHEFTHIVSLQKAMKFGRSIPAIYIQVLDDEP
                     EKREDVVYGYPRIIASYPISGMVIPMWLAEGMAQYMFPGNSNDFWDTHRDMLLRDRVL
                     HKSLLSLSEMGSFGKRGTGNESVYNQGFAFTRYLTEQYGTETIPRLAEELSKPVRMSG
                     ETALKNVTGVAADEIYQQWQTELEQRYFKQTEIIRANEKVGEIILAEGTMQLGPEWSG
                     DSIVYFMSNKGRDYYSQTGLYVYDVRQKKSRMLVPRINSRVTVAPDGQNLYYSRASKP
                     NRHGSVFFDIYRYDLKKRKEKPLTKSARVYNPTLSPDGKRIAFVTGRDGTSNVALMNL
                     DSNKIREITTFSEGVEVFTLHWSPDGRTIAFDYLIGHGRNLALVDVETGAMQILDEAA
                     YDTRNPYFSPDGKWLYFASDETGIYNIYRQSLTTGERQLLTNVTGGAFMPAVNEKGEL
                     VYAILDDRAFKIACIKNPEPIEPQLAVYLDYDQNIPPVSEIQPVSLPEPQKYHDQFAK
                     MFIMPRLMIDYGTVKPGFYCQSNEIINRFNIFAGASINRIKDRDLFLILEYHQWEPTI
                     FLEFYNISRNIFNEKSIYKGWPVEADYTFYLTEAYGGISRPFGGFNNVRFDVKWSRYR
                     TSSDETITAADIYQNGFSYDYYRGVNFQLHWQMRMMLPKVNEETNPDNGILLDTYIAR
                     NYDKFIQGFKVNSSYSTIQVDFRNNYYWRLEHEGNWYHKYPLFKNLVGTLRWHLGWIS
                     KPDVDSFFYFFAGGEPGLRGYPFYSIEGRNLSSWHYTWRLPLFREKHFQILTFNLQNA
                     FVATYIEAGNAWNQVTGFPGLKWEQFKTQPVSVLRNIVNDFKKDIGFQVRMSGFSFYS
                     YPTSISLDFVYGLDDFEVVSNSKTYQYGHEWRTYFTLLFGL"
     gene            complement(9832..12966)
                     /locus_tag="PLI55_00050"
     CDS             complement(9832..12966)
                     /locus_tag="PLI55_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPA99260.1"
                     /translation="MKIYKYLITGITLIVLANKAAYGRFEPQYRIATAPSVVKNTAPA
                     AIRICALRVSFPADEDETTTGTGQFLMSAVETPCDDEFVVDPAPHNRAYFRDHIRSLA
                     NYYNRVSRGKVVIDTTNSEVFPLNDNNSYQVDHPMAYYHPFLVEDSVDLRLTELLVES
                     VQKADPEVDFAQYDVVIVFHAGVGQDFAITLDPTPYDILSAFMNKDDLKAFLAPNDSD
                     FQGIPVENGTAFVDCGLILPESQNHLLYDNWEEVFGGSDSPCHYQFGLNGTLAFMFGF
                     YLGLPCLFDTESGMTGIGKFGLMDQGSANLSGLVPAVPCAWERVFLGWETPVVAKNFE
                     DVVLKKVGSASDTSVWKIPINANEYFLVENRCAHFRPGVSFDSLQYRIYMENGMVELP
                     LSFPLIRDSLKAEFSPETGVMLAVPHYDYGLEGSGLLIWHIDESVINANLATNSVNGD
                     REHRGVDMEEGDGAQDLGYEADILNEDIEDGWFFDPWFAGNDGFIDLNSDYILDVEEH
                     VGFTPYTNPATYSNNYTFTGISIDSIGPAGEVITFRIRLNDALPNFPLSLSAGADEIL
                     PLDLNPNDQVEEFLILADSIRIHTSFGEQIASLPRQTAIALDKDSLLLYGIVQNNPEA
                     PSLSLSIWKFGQNGQINKTDTVSLNSQDLVSPLLPVKGGVLFATQDYADNQNYLHLYQ
                     IDTQNIESLELASQVKMLVGDDDNIFGITDDNQVIRIHLNSLSAEVVADLPNALSGTM
                     VIAFINQNESVDLIHCADSLLTLVLDIGTPNEVVLHREQEFNGNLACADLDDDGKIEI
                     ITATSSQVLAFNEELTVKNNFPIGVPNILGSDRFESELLTADLDNDGKKDVIINMSSA
                     LLAFNYKGDLLSGFPKIAYQQSQKSGILLNTPLGVTYLALTSTESNPQNYNCIKAVRL
                     SSFSHDSEDWICYGGNAARQFYYPVQSQSSPIQSKSLLDNARTFCWPNPPKDNRTYIR
                     YFPNSSCNISINIYDLAGDLIADFKDSAPLIGEPNEKEWDVSNVESGVYFAVVQAKSS
                     SRTYSKVIKILVVK"
     gene            complement(13024..14247)
                     /locus_tag="PLI55_00055"
     CDS             complement(13024..14247)
                     /locus_tag="PLI55_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPA99261.1"
                     /translation="MKRIIAMLGIISLMTAGALAQGEAGAVWLLINPGARFSGMGEVG
                     AALADDAYASYWNPAGLGFLRGRELALTHVNWLPNLVSDIYYEFIGYRQFIRNVGTVG
                     GNIILLNLGEQQRTDEFGHDLGTFNSFMLAVTGSYGTALTRTSSIGVGVKMVHQHLTD
                     QGAGKELGKGYSTNFCFDIAYLKHSFLTKNLTLGINMANIGPKIAFIDVNQADPMPTN
                     MKLGLAYKLGNEYNKVTIAFDINKILVAAYPDRDIDGDEKVGGYDENGHAVPGGEYNK
                     KGQREYAHSDEWYTGIFTSWVDDWLLMYDSATDGIGSADKDGNTNDGSLAAEFEKLTY
                     NFGIEYWYSNLFALRAGCMYDKPGNITMKGKWPVPTFGAGLRWAGMGFDFGYTAGDPG
                     HPRQNTMLFSMNIKF"
     gene            complement(14394..14558)
                     /locus_tag="PLI55_00060"
     CDS             complement(14394..14558)
                     /locus_tag="PLI55_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPA99262.1"
                     /translation="MLSNELLEILACPQCKGELEYDISNQQLICNVCRLKYAIKDDIP
                     IMLIEEAEKF"
     gene            complement(14647..15099)
                     /gene="nrdR"
                     /locus_tag="PLI55_00065"
     CDS             complement(14647..15099)
                     /gene="nrdR"
                     /locus_tag="PLI55_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPA99263.1"
                     /translation="MRCPFCSSPESKVIDSRTVQKNNAIRRRRECIDCGHRYTTYEYV
                     VEYPLQIIKNDGRREEYDRNKLLNSIKIACNKRPIPAEKLETLVIDIEKEIEETSGSE
                     VPSSQIGELVMKKLKELDEVAYIRFASVYKKFKDLDEFKDHINKFGGN"
     gene            complement(15107..16411)
                     /gene="glyA"
                     /locus_tag="PLI55_00070"
     CDS             complement(15107..16411)
                     /gene="glyA"
                     /locus_tag="PLI55_00070"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPA99264.1"
                     /translation="MEYNFLAKQDAVLLDTIKAEIERERQTLEMIASENFVSPAVLEA
                     AGSVLTNKYAEGYPGKRYYGGCAAVDQAENLARDRVKKLFGADYANVQPHSGSQANMA
                     VYFSLVNVGDTVLGMDLAHGGHLTHGSPVNFSGKLFRIISYGVKKDTGRIDFDRLAEL
                     ALEYRPKMIIAGGSAYPRFLDFAKFREIADSVGAFLVADVAHPAGLIAAGLHPDPLPY
                     CHIVTSTTHKTLRGPRGGLILMGKDWENPFGLVAPKSGRTKMMSEIIDSNVMPGIQGG
                     PLMHIIAAKAVAFGEALRPEFKAYAQQIIVNAQVLAEELLHRDYNLVSGGTDNHLILI
                     DLSNKNITGKQAENILEESGITTNKNMVPFDQKSPSVTSGIRIGTAALTTRGMREAEM
                     RTIAGLIDHVLSNPDSKDIHQKVRSEVGELCGNFPLYQEIKL"
     gene            complement(16416..16889)
                     /gene="rpiB"
                     /locus_tag="PLI55_00075"
     CDS             complement(16416..16889)
                     /gene="rpiB"
                     /locus_tag="PLI55_00075"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPA99265.1"
                     /translation="MDHGRTEEELNMVIAFGADHGGYRLKNALIEFARSEGYQVIDFG
                     VNSDDSADYPDYARAVGEAIVSGRAQFGVLICRTGAGMCISANKIHGIRAVMAYQPEI
                     ARLSRLHNHANVLCFGADFTTVESAKEILKTFLETPSSDDLRHLRRVEKIMKLES"
     gene            complement(16859..18145)
                     /locus_tag="PLI55_00080"
     CDS             complement(16859..18145)
                     /locus_tag="PLI55_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99266.1"
                     /translation="MKRWIHFIEEALIVCLVIGLMNCTPPSPESSESVMEQVETKPTI
                     DRDKCDLYLSFAYSYYQNQNWRSAIENYKKMSSFGCEEEYAKDLFPFWGRAYQQLASE
                     NSIYLDSALYVYLAGEKYLPNDLFLKKNIAYIYHLQGKIDLEIREYEKMIEIAPDDIE
                     LYRTLVKLYISAQRYEDVIYATNGILKINPNDEQALNDRIFAYQKLGRDITTIQKEQW
                     EKNPTNIRYGLEYAAALKQQREYDKALEVYQKVAELDAKNIEAWSNIADIYFTQKNNY
                     AEALKAYDQILRNISPNDLNILQQAARCCQLLGDFPKGMEYARKAITIAPNSPQAYKI
                     RADLYFAAADYYTSGRQITFEDKLVYKLAYDDYLKVMELGDTSVKARVDHLKEYLIPT
                     PEEWFFNRYDANGAMRTSFRPLSPEYKWITVEPKKN"
     gene            complement(18164..19387)
                     /locus_tag="PLI55_00085"
     CDS             complement(18164..19387)
                     /locus_tag="PLI55_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99267.1"
                     /translation="MKTSRRTILFLLILSLNGWSQISYYANYGFGVESPANSVRILGM
                     GRIGTAVRDSMSLNQSNPALWQGFGSTSLQGQMYTSALSIPDLDFKGRIVNFSGFSFK
                     LPVGKRAGLAIGLAPLSRMKSSVSFADSLIFQDDKFEYNSVVELSGGISELYLGSGYR
                     VSPNLAIGLRANFLFGNYVVRNHTKIDSDIDSESHYKRTIAVDGSRISFGLLLSDRSQ
                     RYNLAATVEKGNELKRWTIIENNFGPDTTIKSDPINFPTIIKVGINLPIARNLALNAD
                     MQYGIYEEDLFQNFYIFQQFKSDSRNSYAVGIGLERSPRQELKSTFFQKLYYRCGAYY
                     QTEPVYQNSGIYEYGVTAGISLPFFYNLNRIDFALTYGLKNGFLSDEIGNEKILGIHL
                     GITTGEPWFRRYKKQ"
     gene            complement(19384..20634)
                     /locus_tag="PLI55_00090"
     CDS             complement(19384..20634)
                     /locus_tag="PLI55_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99268.1"
                     /translation="MKSSILWNSSKPYFNRVWSKLHYVKIDYRRSVAAFFCLLLFGCS
                     EKIPTLPLDKNLCSFDSVSVSAEFIVDTTVFAEPNIGYSSYLVVGTDANLTAYSLLSF
                     KTLPTPLDTLPLAKILSCELVLMTGSDLSSDTAVHPSLEMTVASIEGEENADWSEDST
                     NSTNFSLDDYHLTDWKTFTYSDADTLILDLTTSFIANWRDSTQSHYGIVIKPAVGSAA
                     GLGIIYSGETNSSPHIIISYITDNGDTISTALACVGDVTITQFKKTFTAPPQPLLISA
                     GKAAYTFLKFQVEELITDKNLFIAGAYLYLHVNSELTEDYGNSHTIYITLLDSAEWES
                     GSFSPSGSSYVTSQTFSASDSILTLKIPSTVQLFTSGNISNFGVALWISPSGLYPAML
                     GFYPTEDPDPARRPRLEILTMQEE"
     gene            complement(20603..21439)
                     /locus_tag="PLI55_00095"
     CDS             complement(20603..21439)
                     /locus_tag="PLI55_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPA99269.1"
                     /translation="MIKRIYFLTSEIIPFAETYQLAIFSKEIPIVFNERKYDFRLMMP
                     KYNFISERRYILREVIRLREMDLQYRGENIKASVKSAFIPNTKVQVYFLEHPYYFNNS
                     DKNLYPTECVSDEYLNSVRFGYFAMAALKTLSYLRWKPEVIICNDWQMSLIPLFIQSN
                     LVDKEYCEGIKILQIIHSKVDCSNFKVTEYQKIGLNNLDLSKSVDGRLDVVAAAMPYC
                     DHVLIINKNENLLEKYQKDPIFSEIMKQDKKKFSSYELNGESPEDWQKLADEIINIME
                     QL"
     gene            complement(21448..22350)
                     /locus_tag="PLI55_00100"
     CDS             complement(21448..22350)
                     /locus_tag="PLI55_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99270.1"
                     /translation="MQQPIKNNLKFKPSAIKLFVLALSLMVINLDLARSDSKRLSNAE
                     IAASLDRLAMDISDLKTQIANLTKTVNSLTAKDKESITKATLTTDINKLIEPLQLTMA
                     NLENRLNILSAEQANMQKRLNTVEQRTRYADSINFEILSQLVILENRLASLSNSISDY
                     NSASQTDARQATNRSITSPGITYKDRYLNALSLHQKGKFEEAIALFRKLIADDPTNEL
                     ADNAQYWIGESYYSMKQYQQAIIEFEKVRNFTNTDKDDDAQYKIGLCFKQMGDQKRAK
                     DELEKLINLYPTSEYSERAKQLIK"
     gene            complement(22435..23685)
                     /gene="rodA"
                     /locus_tag="PLI55_00105"
     CDS             complement(22435..23685)
                     /gene="rodA"
                     /locus_tag="PLI55_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPA99271.1"
                     /translation="MRRSISKRINNFLNSFSNLNLAILVIVGLLCLFGMLSIYSASLN
                     MEGSFFEKIIGRQLIWLVLSGLVAATIYFLQKKLIYDSAYILYLLGFCLLLLPYFLGS
                     SVAGTARWITFGSAGFQPSEFMKIMVMLAIARFLASTSSSNLDFKFVLIPTLLSLLPM
                     AVVLKQPDLGTSIIYFGMLFPMLYWAGARLFHLFIIIAPILSIITAFNFYTFFIWVII
                     VVIILYLTKEKLRVSLTVMLINLSLGFTTPFLWNSLKPYQQQRILTLFNVNADPQGTG
                     YQVLQSKIAIGSGGLFGKGIARGTQTHLKFLPEQHTDFIFSVIGEEYGFIGVLVVLLL
                     FLAFILICINIAYRQKDRFCSLCMIGIASIIGIHVVINVAMTVGLMPVTGLPLPFISY
                     GGSFLMTCFILVGLALNIGVDKQY"
     gene            complement(23666..25426)
                     /gene="mrdA"
                     /locus_tag="PLI55_00110"
     CDS             complement(23666..25426)
                     /gene="mrdA"
                     /locus_tag="PLI55_00110"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPA99272.1"
                     /translation="MTIFFVLVFLVLIGRLYYLQIIKGPSYKGIAEANRVRVIPIEAP
                     RGIIYDRNGKIIVDNKSQYDIDVIPYEFSHTARTDSILAKILGLDVAEINSRIKNNWR
                     GQFVPIKIAEDVNVEIITNIEEHRLDLPGVVYSVKPVRSYPSGVQMSQSLGYVREISA
                     NDLIREKYKEYKQGDLIGWKGIERQYEHILKGTRGYNFIQVDALGREIGQVVDRDNIP
                     PIPGNEMFLAIDLDLQIYIESLTEGYKAGIVVLDADNGEILAIVSKPDYDPALFAGVI
                     SNSSWNALISDPDLPMFNRATQGTYSPGSTFKLIAVLTALETKAVDPDWSVRCYGQYR
                     LGHRIFKCWQERGHGSMNMYEAIVHSCNVYFYNLIRRLDLNDWANMGKDFKFGSETGV
                     DLYGESAGVMPDVDFLNRKYGEHGWTDGNKLNLVIGQGDILVTPVQMARFCAALASRG
                     KLVQPHVGLKYIDRGSNETITFQAAEDSIQNISVDTWEFIEKAMADVINRGTGRAARV
                     KDVPVWGKTGTAQNPHGDAHAWFIGYMKEQNQTIATAVLVEHGKSGGGIAAQIAGQIF
                     NFIHQQKKRQSLANNAPLNF"
     gene            complement(25475..25954)
                     /gene="mreD"
                     /locus_tag="PLI55_00115"
     CDS             complement(25475..25954)
                     /gene="mreD"
                     /locus_tag="PLI55_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPA99273.1"
                     /translation="MLRKILLIIAFSLVTIIIQILLGPWMTIRAIRPDFLLILVLFVG
                     RSEGKVAGQIYGFLIGLIADALGLGSFLGLSALAKTIAGFGAGLLKKRRSHLNPILFY
                     AIEVLIIIIHFSIIYLINFKGAELSTQTIFLRYILPSTLYTGVFYFIIQYFAPFSLE"
     gene            complement(25954..26742)
                     /gene="mreC"
                     /locus_tag="PLI55_00120"
     CDS             complement(25954..26742)
                     /gene="mreC"
                     /locus_tag="PLI55_00120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPA99274.1"
                     /translation="MWLKIIRFIIDHYEYLLLIFLIFLSLFILAHNNSPQIRATQGEL
                     ADAFAFLHYPKVWINELSNLVEENKELKEENLRLALLNAQLKEAYLENQRLREMMNFA
                     DTSRLTILPAEVISQGTTPLYNSIMINVGSKHGVGPNMAVITNDGVVGKTVAVGDNNT
                     LVQIFLDVNFRLGVKFQISREHGIMRWRPNGLAEVREIPKTAIIYPGEKVLTSGFSEI
                     YPPNIIVGEVVEVHQSANNLFQIATVRPSVNINSIEEVFVVLSK"
     gene            complement(26748..27761)
                     /locus_tag="PLI55_00125"
     CDS             complement(26748..27761)
                     /locus_tag="PLI55_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPA99275.1"
                     /translation="MGLNLFNIFTGDIAIDLGTANTIIYMKGKGILLDEPSIVARSTH
                     DGEVLAVGADAKDMLGKTHHDIMTIRPMKDGVIADFEMTDAMIRGFIRKINLSRIARP
                     RMVICIPSGITEVEKRAVRDSADRANAREIYLVDEPVAAAIGIGIDISKPVGNIIVDI
                     GGGTTEIAVIALNGIVTKQAIRVAGDEMDEAIVQYFKTEHNLLIGERTAEYIKCSVGS
                     AMPINDTTIQVKGRSLVAGIPKTIEVSSVEIRECLKDTLDLIIQAIKQALENTPPELS
                     SDILDRGIILTGGGALLKGLDQRIRVETDLPVNVAETPLHSVVKGAGMIIEDPEKYRD
                     VLV"
     gene            complement(27778..29328)
                     /gene="purH"
                     /locus_tag="PLI55_00130"
     CDS             complement(27778..29328)
                     /gene="purH"
                     /locus_tag="PLI55_00130"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPA99276.1"
                     /translation="MSEFKIKRAIISVYDKYNIGNLAQALARKGVQIFSTGGTYRALQ
                     TAGIAVEKIEDLTGFPEILDGRVKTLHPAVFAGLLADATDESHRADLARVKVEPFQLV
                     VVNLYPFSETYHSGNKSFEEIIEMIDIGGPSMLRAASKNFKNVVILSDPGQYDEFLQR
                     LDQDELTLEYRQKLAAAVFLKTMTYDSEISNFLTGAGAEKMPEVIVQAFHKQQTLRYG
                     ENPDQSAALYAPLNNPNWQPFQQLQGKEISFNNYVDCLAAYKCYATFKTERAAVVIVK
                     HTNPCGFAIGDTTLEAYRRAVQTDPVSYFGGIVGVNREIDANLAEEFTKSFLECIIAP
                     SYSNQALEILSKRKNLRLLMPNAEALHYDLDLRSYGQGLLVQEIQAAEDDESQWKIVT
                     ESQPDPSLMPTVRMAWHVVKHLKSNAIVFCDANGTVGIGAGQTSRVDSVKIAIRKAGE
                     AGLSIKNTVMASDAYFPFRDSVDIAAEQEVIGFIQPGGSIRDQESITACNERKLFMIF
                     TGKRVFKH"
     gene            complement(29325..29945)
                     /gene="purN"
                     /locus_tag="PLI55_00135"
     CDS             complement(29325..29945)
                     /gene="purN"
                     /locus_tag="PLI55_00135"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPA99277.1"
                     /translation="MKNIAVFASGRGSNFAAILDKIQKGEIAARVVCVISDNERPPVF
                     EIANAAGIPNFCLNRKQFATPEEYANALLNVLEKYDVDLIVLAGYLKLIPSPVVRKYP
                     RAIVNIHPALLPNFGGKGFYGMKVHEAVIASGVKFSGVTIHFVDERYDTGSVICQEKV
                     AVLPDDTPESLAARVLEVEHRLYPEVVKALCEDKIKFVNGKVVWEK"
     gene            complement(30059..30889)
                     /gene="kduI"
                     /locus_tag="PLI55_00140"
     CDS             complement(30059..30889)
                     /gene="kduI"
                     /locus_tag="PLI55_00140"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HPA99278.1"
                     /translation="MEIRYLADPIRFQRMTTSELRQNMLVENLFQTGELRLVYSDADR
                     AIVGAAVPANKPLTLSGSKELAAEYFTERREIGIINIGEAGIVSVGEESFHLAHKEAL
                     YIGKGRHDIVFQSDSTQKPAEFYLLSYPAHQAYPTRKVTQAQANRRDLGSDAECNKRT
                     IFQYLRPGVLDTCQIVMGLTELAEGSVWNTMPPHTHERRTEIYLYFDLPKNGLVFHLC
                     GEPESTRHIVMHNKQVVLSPSWSIHAGVGTAHYTFIWGMGGENQDFDDMDDIDPVNLR
                     "
     gene            complement(30912..31562)
                     /gene="eda"
                     /locus_tag="PLI55_00145"
     CDS             complement(30912..31562)
                     /gene="eda"
                     /locus_tag="PLI55_00145"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPA99279.1"
                     /translation="MDRQTITAKLIESGLVAVIRLDSGEKLPGIISALRQGGINALEI
                     TMTTPGALEIIKNFAAQTGKDFLIGAGSVLDAETARLAILNGAQFIVGPIFNPEVVRM
                     CHRYDKPAIPGAFTPTEILTAWEQGADLVKVFPTTALGPSYIKDILGPLPQVKLLPTG
                     GVTLENAAEFIRSGACCLGVGTALLNKKMIAADDWQALAKHAEAFRSAVLTGRSSK"
     gene            complement(31719..32474)
                     /locus_tag="PLI55_00150"
     CDS             complement(31719..32474)
                     /locus_tag="PLI55_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPA99280.1"
                     /translation="MAPKNNSQPIYNLFPRIGNPLSLSQEIVEHIEELIRQKKILPGE
                     KLPSETELCKMFGVSRTSLREALQKLSARGLLHIRKGSGIYVTDYQAENVIKPMSLFL
                     ELNLDKDYLMSVMEIRKILEPQIASLAAARRTARDLTKLRKNCNDLKKCDSTDYLKQG
                     QIDKDFHLNIAIASKNQLVPIIVEPMFRLMPKIRSLVYQQIDSAKSSAITYHDLILEK
                     IQVKDSQGAFEAMTTHLQIAEEHTLQIITDLAK"
     gene            complement(32721..33491)
                     /locus_tag="PLI55_00155"
     CDS             complement(32721..33491)
                     /locus_tag="PLI55_00155"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HPA99281.1"
                     /translation="MQEITGYPGAIRFSIEKNNGETPSLAQIFQMRQNNKQLISPNEQ
                     YHQLVLFQHFLRELTAALKYNILKNTSVAFRMRVELANLLKENRALIQPAAIVRLVDC
                     LPTETGLALAGGTYELHGKSLHRFVNKSDPVKPKPIFIFIVTIGSAIDKRIADLNYTE
                     GDAYQAFLLNGLAAALVETATSDLQQYLEKSSSWLSPSQTLRRVSPGFRDWPLTDQKV
                     IFEILKPSENIGVTLSADCLMQPLKSTSGIMGVIENAL"
     gene            complement(33683..34306)
                     /locus_tag="PLI55_00160"
     CDS             complement(33683..34306)
                     /locus_tag="PLI55_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPA99282.1"
                     /translation="MSEHPYFIGIAGNIGVGKTTMTDLIARRFGWRAFYESVIDNPYL
                     EDFYQDMKRWSFNLQIYFLSHRFRSHKQMTESNITTVQDRTIYEDVEIFARNLYEMGN
                     MSQRDWDNYRALFEIMTSYLKKPDLIVYLRASVDTLLTRIKKRSRGFERDISPEYIYT
                     LNLSYERWIKEEQKRSNILIVETDQFDIFRDQEKLEEIMQKIKQFCP"
     gene            complement(34299..35594)
                     /gene="mtaB"
                     /locus_tag="PLI55_00165"
     CDS             complement(34299..35594)
                     /gene="mtaB"
                     /locus_tag="PLI55_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPA99283.1"
                     /translation="MIATVAFRTLGCRLNQAETDAIKAELANRNLHIVNSNKPADLTI
                     INSCAVTQAAEAKTRGAITAARRISPNGKIAVIGCYSQIAAAELLNISGVFLVLGNNE
                     KYEIFDFLETIERGEKIVRVTKPESPKTFRIESHLPNDKRIRANLKVQEGCDYYCAYC
                     IIPHLRGNPRSRDPEDCIATARLLENQGVQEIVLTGINLGRYESPSGDLFFLIRRLLA
                     ATGLPRIRLSSIEPDLITSELIKLISEEPRLCRHLHIPLQHGSDPILAKMNRRYLSAD
                     YATLINRIKQQIPEICIGADVMVGFPGETDLEFDQMRQFLENLDINYLHVFRYSPRPG
                     TPAGKFTDRIPDSEKKRRSEILRQLSNRKRAAFRQSMLAKTVSVLFEEKIAPGLWQGL
                     TDNYLKVRIHTRENLLNKIEKVKLIHVSEDYIEGELLGE"
     gene            complement(35591..36661)
                     /locus_tag="PLI55_00170"
     CDS             complement(35591..36661)
                     /locus_tag="PLI55_00170"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HPA99284.1"
                     /translation="MKALITAGGHGTRLRPITHTQNKHLIPIANKPILNYALEYVRDA
                     GIQEVGIITNKEGNEVREVYGDGQAFGLKIVYIPQERPTGLADCVRIAEPFIGNEPFV
                     FYLGDNIIIGGIRRFIEEFERNQTNCHLVLAKVPDPERFGVPEIKGNRIVSIVEKPQK
                     PLSDFAVTGIYIYDHTIFEAVNNIQPSARGELEISDAHQYLLNKGYKVTFSEITGWWK
                     DTGKPSDLLEANRLVLDNITTSIQGKVDQNSQLSGRVVISKGAEIINSNIRGPVIIGE
                     GAKIVNAYIGPYTAIDKNCEISNAEIEFSIVSCNCVIRNQKIRIEASLLGKNVQIIES
                     QAKPFTNRFIIGDQSIIEIRQT"
     gene            complement(36738..38042)
                     /gene="rimO"
                     /locus_tag="PLI55_00175"
     CDS             complement(36738..38042)
                     /gene="rimO"
                     /locus_tag="PLI55_00175"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPA99285.1"
                     /translation="MSLIPAVRSFAIISLGCPKNTVDSEILKGGLMQAGMEFKADPAE
                     ADAVIINTCGFIQPAKEESIETILETLRLKKTGVKKVVVMGCLSQRYYAQIKAEIPEI
                     DGLFGVESQSEVIRFLTGSQNICPDLETIRQLMTPRHFAYLKIAEGCDNSCSFCAIPL
                     IRGRQRSRSIESLLREAEYLKSQGVKELILIAQDTTRYGSDLPGKVSLKTLLEELLAA
                     RLFPWLRLMYANPDFWRDDLNHILAQHPEFCPYIDIPIQHASPRLLKLMNRGANPKRL
                     KATLRNIRRYRPDVALRTSVMVGFPSETEAEFEELLNFIEEIRFERLGVFTYSAEEDT
                     VAEKFDDDIPEPEKERRRDLLMQIQWEIGEDFARRKIGQKITVLIEEQDGDEYIARSV
                     WDAPEIDCNVRVKSPRILSIGDFYEVTIIGVENLDLRAETNG"
     gene            complement(38139..39023)
                     /gene="ftsY"
                     /locus_tag="PLI55_00180"
     CDS             complement(38139..39023)
                     /gene="ftsY"
                     /locus_tag="PLI55_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPA99286.1"
                     /translation="MINFGLNKTGRFLASRLPLLGRFFRLDEKTLEEIEETLIQTDLG
                     VELTETLIERLRKVDRKEAARLNEILQNELSALIQPAVATKTNFHKPTVILIVGVNGT
                     GKTTTIGKLAAKYASEGQRVLICAADTFRAAAYEQLRIWAERSGVEFIGNPQGKDPSA
                     IAFDAARAAQARNADILLIDTAGRLHTKSNLMEELAKVKRVIGKVIPDAPHEVWLVLD
                     ATVGQNGILQAREFQKAIGITGLILTKLDGTAKGGIALAIHRELNIPIRFLGVGEKLT
                     DLVEFDPNQYIRDLLNIE"
     gene            complement(39016..39654)
                     /locus_tag="PLI55_00185"
     CDS             complement(39016..39654)
                     /locus_tag="PLI55_00185"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPA99287.1"
                     /translation="MSKNPIKHTLQVKRNITSKYDAIPARKTVTISNIISLVRAFLAA
                     PIVYSLRNNDSKTALIFILIAILSDMLDGWLARSSNEITVLGKILDPFADSVVIMSVM
                     LFLTVKERIPTFYFTIMLVRYFLISVFGIYMLNNSKVSPQSNKLGKVSVVFTVITVLA
                     FIYPNFFGSSAKPLMIVSIIFMALSLVQYIYEYSRQIIIASREKKANLSKND"
     gene            complement(39647..40396)
                     /locus_tag="PLI55_00190"
     CDS             complement(39647..40396)
                     /locus_tag="PLI55_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HPA99288.1"
                     /translation="MNYLICQNLEFQYPQNPNFRLSIPELTCPLNYPLGVYGLTGSGK
                     STFGKILGGLLTPTKGFFQIEAPEPNLSSSKTKPRILYLPQFPEQIFLGITIGNALQK
                     ILKSWNDPAELTAKFADNLQKLGLDYNLIRERYGYELSSGELRLATIALGITLAPQLT
                     IFDEPTIALSPSSRTKFENILPEYDSQKEFMIISHDYRLLRKLCHNILIFDKGSVIFE
                     GEWKILDQNFSIIHKVGLNLLEKSYQRNSNE"
     gene            complement(40415..41062)
                     /locus_tag="PLI55_00195"
     CDS             complement(40415..41062)
                     /locus_tag="PLI55_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA99289.1"
                     /translation="MKLRLDGIDFSYHGNQPVFKNLDLTIPLNCWAVLTGDCGTGKTT
                     LAKLIAGLLKPDKGSIRFENDNGLNNSLEVGYIFQNPDDQFVHFNIEREVAFNLENRG
                     ASPAIVRKAVQLALESVNLWPRRENSPHELSGGEKQKLAIADWLVSKPQVLIFDETTA
                     FLDLPTRFELYENIRRLIVAGLSVIWISQEDYEIAFADYIIELLPGYLLRTSSNK"
     gene            complement(41059..42339)
                     /locus_tag="PLI55_00200"
     CDS             complement(41059..42339)
                     /locus_tag="PLI55_00200"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPA99290.1"
                     /translation="MTIFWLVIYNAIVMPIAYSGLMILSHFNSKIKEGLKGRQTLWLN
                     LQKFREELPKRTVYLVHSASLGEFEQAKPVIRGLKTLRPDILLVASFTSPSGFKNAER
                     MPEVDLFTYLPIDTFLQTRRFLQKLRPTKIIFVTYELWPNLIFHANRYKIKTYLISAR
                     IRPDSSKLKPIIRHFFADLYRSLDYIYAISEEHKQSVLKLIGSVKITVLNLGDTRYDQ
                     VIERSKLLANKMVTRVFNDGFVFIGGSIWPQDARPLLPALYQCLKNFPDFKIILTPHE
                     PNDYALEMLEDEFQTHNIETVRYSQLKGMPANVRVVLIDRIGILAELYHQANLAFVGG
                     SFRGSIHNVMEPAVAGLPVLFGPSYHNSQEAELLVQSGGGLVCLDEPGIYSTIKHLIT
                     KPDDYQKAAQSARQVILKNMGASSRTVQEILESE"
     gene            complement(42336..43379)
                     /locus_tag="PLI55_00205"
     CDS             complement(42336..43379)
                     /locus_tag="PLI55_00205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPA99291.1"
                     /translation="MTTTTNPNSILIMRLSSMGDIVVLTPIFRSLREKYPLARIDFLT
                     KMEFAEIVANNPFLSNIITYDSGTKLAGWRDLCRQLKAQDYDLLIDMHNNIRSHILSH
                     YLRGTSLLRYHKPYLRRFLLFYFWLNFFSHDFRLLKEYYKVLQPLGINYPPTAKPEIF
                     LEPEAVTKAQDILVKYEISEPFIIILPIANWPNKLFPLEKYINVARRINTDLQMPVLW
                     LGGKADIHLQQLDYLVPGKSVRLAGETTLSESLALIGLSRAVIGNDTGLTYAAEALGK
                     PVILILGPTARETGAGCYAPKSIVLEAQLFCRPCSQKGDRRCYRRQRQLCFNQITDAQ
                     VYDALVKIIGAES"
     gene            complement(43376..44356)
                     /locus_tag="PLI55_00210"
     CDS             complement(43376..44356)
                     /locus_tag="PLI55_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPA99292.1"
                     /translation="MPPKDLNNIFAPISTELDLFQKEFRQVLYSEVELINQIGSYILS
                     GGGKYIRPALVLLSANLCGKSNEHSIIAASVVEILHTATLVHDDIIDDSEVRRGRPSV
                     KAIWSNKIAVLAGDFLFSRALSNMLRLKDFTVLELLSQTSELLSSGELLQLEKSLNNN
                     MDEEIYYQVIWAKTASLFATACQVGTLTVAANEEQTKALFDYGRCLGLAFQIEDDLFD
                     CTGTSIHTGKPVARDIKQNLVTLPLIHALKTLAPEERHRIRQQLKNRPSPEQITAITE
                     LIINTNGITYAENKCRELIQQAVAALAIFPDSQYKSALINLANYTLMRNN"
     gene            complement(44363..45136)
                     /gene="tatC"
                     /locus_tag="PLI55_00215"
     CDS             complement(44363..45136)
                     /gene="tatC"
                     /locus_tag="PLI55_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPA99293.1"
                     /translation="MARINSTNAEKEMGFLDHLEELRWRIIKILIALVLGTVGAFLFS
                     DQAIDILMRPSRQVGSEMTIQVLKIQGLLMLKFKVAFLLGLVIALPIVAYQFWAFISP
                     GLLQNEKRWGPGLIIGVTVFFLLGAIFAFFVLIPIALKFLIGIGVAGVERNISIEYYT
                     KFVMQLILATGLIFQMPVLSFILTKIGLITPQFLRKSWRYAIIILMVIAAIITPPDPV
                     SMIFMTLPLLFLYEISIWISRIASRPKIQNETDQIELAG"
     gene            complement(45408..46085)
                     /locus_tag="PLI55_00220"
     CDS             complement(45408..46085)
                     /locus_tag="PLI55_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPA99294.1"
                     /translation="MEIDILKNIPLFADLKDDIIQQIYNLMQKRAYKKGNIILMEEEF
                     GDTLFILSKGSVKITRMGEDGREVILSILGEGDFFGEMSILDGENRSANVVALEDTAV
                     LILKRGDFLDLLEKHPQIAIALLQEMATRLRRSDQQIEGLSLSDAENRIAMCLLRLAE
                     DLGVIKQGKVIIENLPYQQDIANMAGTSRETVSRMLTLLQRKGYIQKYGRRLIINNYD
                     GFVKQFS"
     gene            complement(46180..46551)
                     /locus_tag="PLI55_00225"
     CDS             complement(46180..46551)
                     /locus_tag="PLI55_00225"
                     /inference="COORDINATES: protein motif:HMM:NF021119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HPA99295.1"
                     /translation="MLEGLVDTLLSRLREIVASETVIGKPIETGDTMVIPITKISLGF
                     MVGSSGNPEEQKKKTEGTGLGGGALIEPLAVITIQKGEVKIHRLKEKISGVDKLMEIV
                     PEVLEKYVKPKSEKKSAKENT"
     gene            complement(46555..47196)
                     /locus_tag="PLI55_00230"
     CDS             complement(46555..47196)
                     /locus_tag="PLI55_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99296.1"
                     /translation="MMIILKILLVLFFILIFLLFVTLFSKTWLSIFGNFSEQTKTGTL
                     KVQLAGKLSSIIVVMETPEVNFRIGILGLHFKVGQKKAPSLAKKKSPEPKPEIPKKPR
                     TRRYKFSIFDWIEFGKALTLRFFKLVHIETLSANLTVGLGNPAYTGLLMGAYYTIRET
                     VKDFQSVHIVPDFVAKKFTGKVEFRASIRLIKTIPILIFIVNFLRKMKNKKRR"
     gene            47282..47536
                     /gene="rpsT"
                     /locus_tag="PLI55_00235"
     CDS             47282..47536
                     /gene="rpsT"
                     /locus_tag="PLI55_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPA99297.1"
                     /translation="MKKNASVLKRTRQAQKAALRNRHYVSMMKTSIKKVKNTKTKAEA
                     EPLFKAAVALIDKVARKGIIHKNNAAHRKSALAEYLANLS"
     gene            complement(47564..49027)
                     /gene="guaB"
                     /locus_tag="PLI55_00240"
     CDS             complement(47564..49027)
                     /gene="guaB"
                     /locus_tag="PLI55_00240"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPA99298.1"
                     /translation="MNEKIAREGLTFDDVLLIPSESNVLPKEVSLDAKLTKKIQLNIP
                     ILSAAMDTVTEGKMAIAIAREGGIGILHKNMSIEQQVAEVDKVKRSESGMIVKPITLS
                     SDSTIRDALNLMNRYSISGIPVVDNGQLKGILTNRDIRFETNLDLRISERMTKENLVT
                     APEGTTLEEAEEILQRHRIEKLLIVDDKGRLKGLITVKDILKKKRFPNAAKDEFGRLR
                     VGAAVGVGSDYLLRAEKLVSEQVDVLVIDTAHGHSTNVIKSVRELKRRFPQVDVIAGN
                     VATAAATKALIDAGADAVKVGIGPGAICTTRVIAGVGIPQLTAILDCAEVAMKSGIPI
                     IADGGIRYSGDIAKALAAGASTVMLGSLLAGLDESPGETVLYEGRVYKSYRGMGSIAA
                     MTHGSSDRYFQEGEETSKLVPEGIEGIVPYKGSLADTIHQLIGGLRSAMGYCGTATIN
                     ELQKRGCFIRVTQASIQESHPHNVKISKEAPNYRLEN"
     gene            49445..49852
                     /locus_tag="PLI55_00245"
     CDS             49445..49852
                     /locus_tag="PLI55_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99299.1"
                     /translation="MQSMKKIFLIWMFSTIAFAQQAVEVSDIIISDLDNNLIEIRVEV
                     KNTSMKSISELAGFIDIYDKYGNIIEKQEIVIVLNSDIPLKPQNTASGSIIITQRPNM
                     SGQVNFRITNLRYFGEPNVYLICPVCGELIPKD"
     gene            50023..52032
                     /gene="ligA"
                     /locus_tag="PLI55_00250"
     CDS             50023..52032
                     /gene="ligA"
                     /locus_tag="PLI55_00250"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017888964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPA99300.1"
                     /translation="MNLDALREKAKELRRQIETANYNYYVLDNPTISDAEYDRLMREL
                     EQLEAAYPELRTPDSPTQRVGAQPLDEFKTVTHRVPMLSLDNAMDEGEIRDFAIRVQK
                     SLPGETVQFVCEPKIDGLAVELVYENGVFISGSTRGDGITGEDITQNLKTIRSIPLRL
                     MPNLPIPTLLEVRGEVYMDKADFQRLNEQQLKEGKPLFANPRNSAAGSVRQLDPQITA
                     KRPLKIFCYGLGSLEGYSFQTHHEFLEALLKWGFRVNPLIEIAKEVEALIAYYHKIES
                     IRDSMPYDIDGVVYKVDSLAQQAKLGIKSRSPRWAIAGKFKAQQEVTQIIDIEASVGR
                     TGAITPVAHLKPVQIGGVTVSHATLHNQDEIDRKDIRIGDWVVVQRAGDVIPQVVKVI
                     TERRTGNEKRYSIPANCPVCGTPVVREEDEAKHRCPNINCPAQIKASIEHFASKRAMN
                     IEGLGEKLIEQLVDSGLIKNVADIYSLRKEQLLTLERMGEKSAQNLLDAITASQNVSL
                     ARFIYALGIRNVGEHMAKVLAKAFGSLERLSQASEEELMAIEGVGPIVARSVRNFFNA
                     ESNRQTIEKLLSSGIRISSEQDYKQKEEIAGKTFVFTGTLTNFTREEAQALVEKYGGK
                     SASSVSKKTDYVVVGENAGSKAETARQLGVKILNEAEFLELISYN"
     gene            complement(52206..52802)
                     /gene="hisIE"
                     /locus_tag="PLI55_00255"
     CDS             complement(52206..52802)
                     /gene="hisIE"
                     /locus_tag="PLI55_00255"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014269959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HPA99301.1"
                     /translation="MNIDFTKNNGLLPAIIQDEKTGKVLMLGYMNRESYEKTKATGRV
                     TFFSRSRQALWTKGETSGNFLEVKEILSDCDGDTLLIKVKPTGPTCHTGADTCFNEKN
                     IAEKPFIFELEEIIRDRRNNPKPGSYTNKLFDAGDERIAQKVGEEATEVVIDAVTGNE
                     MRLPEEVADLLYHLLVLLVAKDVALDDIMNVLEKRHRK"
     gene            complement(52799..53554)
                     /gene="hisF"
                     /locus_tag="PLI55_00260"
     CDS             complement(52799..53554)
                     /gene="hisF"
                     /locus_tag="PLI55_00260"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPA99302.1"
                     /translation="MLAKRIIPCLDVKDGQTVKGVNFINIRRAGDPVELGAAYAEQGA
                     DELVFLDITASIEKRGTFIELVREIAKHLNIPFTVGGGVRNADDVARLLDAGADKISI
                     NTAAVNHPEMIEKLAGRFGSQCIVIAIDTKHTENGDFVYINGGRTPTNQTTQAWARQV
                     VELGAGEILLTSMDHDGTKRGFALEITRELAENLPIPVIASGGAGTMEHFWEVFKIGK
                     ADAALAASVFHFGEIPIPELKKFLKSNGIEVRL"
     gene            complement(53548..54270)
                     /gene="hisA"
                     /locus_tag="PLI55_00265"
     CDS             complement(53548..54270)
                     /gene="hisA"
                     /locus_tag="PLI55_00265"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPA99303.1"
                     /translation="MITIIPAIDIINGKCVRLSRGDFNTQKIYNENPLEIARQFADAG
                     IRRLHLVDLDGARQKRVVNWEVLEKIATGTPLQIDFSGGIRSETDVRIAFESGARQIT
                     AGSIAISAPDLVEEWLKKFGGEKIILGADVIDRKIAIHGWQEKAPLEIMEFLQNFQEK
                     GIQQTICTEVARDGMLTGPAFELYREIKQIFPDLYLIASGGISRIDDIYQLQDDGIDG
                     VIIGKALYEGYLKLSELKGFLC"
     gene            complement(54267..54848)
                     /gene="hisH"
                     /locus_tag="PLI55_00270"
     CDS             complement(54267..54848)
                     /gene="hisH"
                     /locus_tag="PLI55_00270"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPA99304.1"
                     /translation="MKVVIIGYNSGNIHSVRLALQRLNVKAVVSSDPAVIQAADKVIF
                     PGVGEASSAMESIKANGIFEILPRLQKPTLGICLGLQLMCRDSEENATQCFGIFDIAV
                     RKFPPLAKVPQVGWNTLFDLKGDLFRDVAENSYVYFVHSYYAELSEHTVAQTEYIIPY
                     SAAMQKDNFFAVQFHPEKSGKVGEQILRNFIKL"
     gene            complement(54845..55936)
                     /gene="hisB"
                     /locus_tag="PLI55_00275"
     CDS             complement(54845..55936)
                     /gene="hisB"
                     /locus_tag="PLI55_00275"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HPA99305.1"
                     /translation="MKKVLFIDRDGTLIIEPPDEQVDSLEKLEFLPGAITNMNKIATQ
                     FDYQLVMVTNQDGLGTDLFPEKDFWPAHNKMLQILAGEGVVFDQILIDRSLPNDNAPS
                     RKPGIGMLTDYLKGDYDLENSFVIGDRETDVQLARNLGTKAIYLGSNESISADFKAKN
                     WSEIYNYLSNISRTATVTRKTNETDIRLVINLNGKGANRIDTGIGFLDHMLNLFATHS
                     GCDLDLTARGDLAVDEHHTVEDVALVLGEGLLKALGDKKGIERYGFLLPMDESLAQVA
                     LDFSGRREFVWECDFKREYIGKMPTELFRHFFKSFADSAACTLHIKATGENEHHKIEA
                     VFKAVGRAVKMAIRRDPTCDQIPSSKGKL"
     gene            complement(55933..56979)
                     /gene="hisC"
                     /locus_tag="PLI55_00280"
     CDS             complement(55933..56979)
                     /gene="hisC"
                     /locus_tag="PLI55_00280"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPA99306.1"
                     /translation="MVDLEKLARRNIINLQPYATARDDYSGAIGIFLDANENSLGSVI
                     PQKFNRYPDPHQKRLKQEIAALKNVNPEQVFLGNGSDEVIDLLIRVFCQPGTEKIMIL
                     LPTYGMYAVSAASNDVGVVSVALTPNFQINLPQIFEQIAGVKLIFLCSPNNPTGNCLN
                     SADVVNLLEKFKGIVVIDEAYIDFAPQKSFLPRLKEFNNLVIMQTFSKAWGLANLRLG
                     MAFADPQIIDWLSRLKYPYNVNGLTQELALSALERSAEKDRMVTELRTERERLADSLK
                     ELPIVEKVYPSDANFLLVKFRDARSVFQYLINQKIIVRDRSQAWHCENCLRITIGTPN
                     ENDILIAKLKDFAS"
     gene            complement(56972..58273)
                     /gene="hisD"
                     /locus_tag="PLI55_00285"
     CDS             complement(56972..58273)
                     /gene="hisD"
                     /locus_tag="PLI55_00285"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPA99307.1"
                     /translation="MKVIKYPQKTSWPELVRRPAIDNKILRNLVIEIIEAVKTDGDDA
                     LRQFSWKYDNVRLKEIEILRDELASVPNEVDDDLRKAIEIARANITKFHSSQFEGIEK
                     VETTPGVICWRKSVPIESVGLYIPGGSAPLFSTVLMLGIPARLAGCPEIILCTPPNKD
                     GRIHPVILYTAQILGIERIFKIGGAQAIAAMAYGTQSVPRVAKIFGPGNQYVTIAKQI
                     VSAQGVAIDMPAGPSEVAVVADRFANPDFVAADLLSQAEHGADSQVICVTDNEELIPQ
                     VITAVKQQLAELPRRTIIEKSLESSRLILVNNLDEALEFINCYAPEHLILQTQNPEQW
                     ADKVINAGSVFLGDFTPESAGDYASGTNHTLPTNGYARNYSGISVDSFVKKITFQQIS
                     PAGLKAIAPAIDCMATTEKLQAHRNAVRIRLEAIEKGAGRG"
     gene            complement(58283..59137)
                     /gene="hisG"
                     /locus_tag="PLI55_00290"
     CDS             complement(58283..59137)
                     /gene="hisG"
                     /locus_tag="PLI55_00290"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPA99308.1"
                     /translation="MITLAIQKTGRLNEHTTKLLSECGLFVSNGSTARLMTEASNFPM
                     RILFLRDDDIPECVADGLADLGIVGENVYHEKARPLKIVERLGFAKCRLSIAVPKDFS
                     YEKLTDLNGLSIATSYPNILKRFLNENNLKADIHEISGSVEIAPGIGLASAIFDIVST
                     GSTLLTNGLKEVVTVLKSEAVLVAAENLSPEKTDLLEQLLLRIRAVQAAHTRKYILLN
                     APDSAIAKICAILPGMKSPTIMPLAEPGWSSLHSVIEEHQFWERISALKAAGAQGILV
                     IPIEKMIM"
     gene            complement(59352..60032)
                     /locus_tag="PLI55_00295"
     CDS             complement(59352..60032)
                     /locus_tag="PLI55_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013404253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HPA99309.1"
                     /translation="MKSFYLEPLPKCDISRLSGKLIVLEGSDGSGRTTQITRIKSWLE
                     GKGYAVVNCELARSQLVGPELELARRGNTLCPTTLSLFYATDFMDQLENVIIPALRAG
                     FIVLADRYFYTLMARDLVRGTDLKWVQNIYGVAVVPDLVFYLNVSPHRLIERRWQKAN
                     AMDYWESGMDMGLSRDWAVSFLIYQQYLRKVFLKLQDMYGFIVINGNKSITAITNDIV
                     KQIEKVLI"
     gene            complement(60029..60715)
                     /locus_tag="PLI55_00300"
     CDS             complement(60029..60715)
                     /locus_tag="PLI55_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HPA99310.1"
                     /translation="MNYPKSKRSSGILIAAEGLDGSGKSTQLYLVKRWLELSGHHVYL
                     TEWSSSDMVRQAIKRGKKKNLLTPTTFSLVHAVDFADRYHRQIQPLLKTGFITLSDRY
                     IFTAFARDAVRGCSSAWLRNLYSFAKIPDITFYFKLSPQIAAERILSNRPKIKYYEAG
                     MDLNLHPEVYDSFRIYQGKIGTEYDQMSQEFGFVEIDATQPIKTQQEKIRAIIAEKID
                     LASYKKGELE"
     gene            complement(60817..62154)
                     /locus_tag="PLI55_00305"
     CDS             complement(60817..62154)
                     /locus_tag="PLI55_00305"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HPA99311.1"
                     /translation="MPSVKQMKRTKNFIMVDLIEKKKAGLEHSKEEIQYLIASYTKGK
                     IPDYQMAAWLMAVNFQGMTDAETFALVETMLLSGTKLDLSHIKHCKVDKHSTGGVGDK
                     VSLILAPLAVAAGCVVPMISGRGLGHTGGTLDKLESIPGFNVNLSSQEFVRLVEKYGL
                     AMGGQTEELVPADRKIYALRDVTATVNSIPLICGSILSKKIAEGIDGLVLDVKTGNGA
                     FLADYNQSQQLAKQLKRVGENFGLQIKAVITDMNQPLGRAVGNWLEVVESIVTIKGQG
                     PEDLTTITLDLTAWMVVLSGIEKNFGEARELLKQLIISGEAYQKFLLMVEAQGGDISY
                     IEHPEKYKQAAWQAPVLATESGWVKNIDTKRIGMCAVALGAGRQSLDSVIKPEVGFII
                     NKKIGDYVEVGEPLVTVHAGSRRAFQNVQSAIRKAYHFSKAPVTPPPLFYETF"
     gene            complement(62174..63622)
                     /locus_tag="PLI55_00310"
     CDS             complement(62174..63622)
                     /locus_tag="PLI55_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPA99312.1"
                     /translation="MYIILVKRSFFKKIGRLCWFSTGWIGARYIYRELIFPFLFSIGV
                     VTFILLANFLIKSVDRLLGKGLPFGVIFEFLYLNLAWIVAMSVPMAVLVATLMAYGRL
                     AEDNEITAMRSSGISFTTILVPGFIFGLVVSVVMLYFHDGVLPEFNHRARLLTSDIYR
                     KRPDLNIEPGYFIDDLPDYSIYVKEKVGESLRKVTIYNKDARDIQTTIYADSGYIGVE
                     GNTILFTLFSGQIHEIDLKGLSDYRRIDFERHRIAIPVDNLMLERRESERRGDREMTI
                     KMMRTEVERYSREYQQVIAKINNLVRKELGDTLTSFDLKKIEKKVQTLQAENLKNLPP
                     AEAARRNQKLTIFLNRIRSESNLAFNYKKQIAKYNVEIQKKYSIPFACIVFVLLGAPL
                     GVITRRGGITVSAVLSIFFFLLYYVFLIGGEELADAAIISPFWAMWTPNLILLIVGIY
                     LIYYTSSEQRVIDFSRFPYLFRRLNKKIKKNP"
BASE COUNT        18120 a        14043 c        13552 g        18127 t
ORIGIN      
        1 attgatttga aattacctac aatttataag gagtaactat ggcagtcaat attgttgaag
       61 tggcaactcc ccgacaatta aaacagttca taaagtttcc actgaagctt tatcgtaata
      121 atccatatta cataccacca ttgaattttg atgaaaggaa tacgctggat ccacgccgca
      181 atcccgcctg tgaattctgt gacacgcgtt actggctggc atataaaaat ggcaaattgg
      241 cgggccgcat tgccggtatt tacaataaac gctttgttga aatttgcggt aagaaatatt
      301 tacgattcgg ctgggtggat tttatagatg atgaggaagt tgttgtggct ctctttaagg
      361 cgctcgagga ttggggcaaa gagctgggag tcgaagctat tcatgggccg ctgggtttta
      421 ctgatctgga tcgtgaggga atgctgatcg aaggctttca agagattgga acgatggcaa
      481 ccatttataa ttatccttac tacccgaaat atcttgagca gttaggatac caaaaagata
      541 tcgattgggt cgaatacgat atcactgtac caaaggttat gccccagaaa ctcgttaatg
      601 tttcccagat tgtcaaacag cgacttgggt tacatacttt gcagatcaag aaaatcaagg
      661 atgttttacc ttatgcccat caaattttcg aggtcttaaa tgcgacttat gccgttctgt
      721 acggctttgt accacttacc gaaaaacaaa ttgataccta tgtcaagcaa tatattagcc
      781 tgatgaaccc cgattttatt gccattgtgc tcgacaaaaa cgatcaaatc gttggaatcg
      841 ggctcacaat gccatcttta gcccgtgctc ttcagaaagc cagaggcaag ctttttccat
      901 ttggatttgt gcacctgtta tgggctttga gacatgctaa agtagccgat ctgttgatag
      961 ttggcgtttt gcctgaatat caaggccagg gagtgccggc tttaatgatt gcggaaatag
     1021 gtatgaaatt aattcgtaaa ggcatacaga gagccgaatc gaaccccgag ctggagacta
     1081 atatcaaagt ccaatctcag tggaaatatt tcgatttgcg tcaacataag cggcggcgtt
     1141 gctatattaa acatgtctaa accctctcaa gtaaatctaa gacgctaaaa ttgtttgcca
     1201 agaaaactga tatcggaaat cagccggtta gaagtctgca gaatgtacgg catattattc
     1261 tggatttaga cggcacaatt tataatgggc acactctttt cccatttatt aaagattttc
     1321 tcgatgattt aaaaaatctg caagttgatt atactttttt aactaataat tcttctctga
     1381 gcgtgacggc ttatatccgc aaattacgtc gaatgggctt gccggttaag ccaacgcaag
     1441 tctatacttc tgctcaggca acgattgatt atttgcggcg tcgtcacccg gctatcaagc
     1501 gcatatactt agtcgggacc cagagcctga gacgtgaatt tatagatagc gggtttacag
     1561 tggttggcga cgatcctacg aacgaaccgg atgccgtaat cgttgggttt gataccagtc
     1621 taacgtttca aaaactgtgt ggcgcggctt actggataaa aaccggcaaa ctcttcatcg
     1681 ctactcatcc cgatctaact tgtcctactg atcaggatac ggttctggtg gattgcggtg
     1741 cagtttgtgc ggcattgaaa gcggctaccg gcaagaagcc atcggcagtg ctcggcaaac
     1801 ctgacccgtc aatgataaaa ggcattatgc aaaggttcgg attaaaaaag gatgaagttg
     1861 ccattgttgg cgatcgccta tatactgata ttaaaatggc acgacaggca ggaatcgcca
     1921 gtgtattggt attatccgga gaaacaacgc aggctgatct acaagaaagt cccattttac
     1981 cagactatgt tctcgaaaat gtcggcgaat tgtccaaaat tctaaggcaa agtcgcaagt
     2041 agaagaggtg ttccaatagc aggaattgct attaatgtaa cattgatttt ggtcaggggg
     2101 cacctttttt tagataaaat agacaaatgg cattaatata gattttcaga tcaatgataa
     2161 cttttgattt atttttctgg gagaattggc gggatcgacg ggactcgaac ccgcaacccc
     2221 cggcgtgaca ggccggtgct ctaaccagtt gaactacgac cccttttaaa tagcgatggc
     2281 aaatttaagt aattttccaa gtcaggacaa gcatatttaa gtcatttacn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttttaaaatt atgtttgtta tagatttttt
     2461 tctatattcg ttagtgatga aattagacgg tcatcaggag caataaatgg gttttaaatg
     2521 cggaattatc ggtttgccga acgttggtaa atcaacatta ttcaatgccc tgacgcatgc
     2581 cggcgtcgcc atggagaatt acccattctg tacgatcaat ccgcatatcg gagttgtaaa
     2641 tgttccggat aagcgactcg atttgcttgc ccagatttat aagccggaca agatcattcc
     2701 gactacgttg caattcgtgg acatcgccgg gttggtgcgt ggcgcttccc aaggcgaagg
     2761 attgggtaac caatttctga gtcatattca agcagtggac gccattatcc acgttgtgcg
     2821 atgctttgaa aatcccaatg tagcccacat tgatgaaaaa cttgatccgc aacgcgacat
     2881 cgaaatcgtc gaaaccgaaa ttttattaaa agacctcgaa attgtggaaa accgccggga
     2941 aaaattaatt gcccgagcca aaagcggcga tgagcaaacc caaaaacaat taaaaattct
     3001 ggatgatatc cgcggtctgc ttatcaaagg gcagtctgcc aagcaatatc attctcaccc
     3061 ggaagaagaa tatttcatac gtgatttggc gcttttgtcc agcaagccag tcatcatcct
     3121 gggaaatatt tccgaagatg aagctatatc ggggaataaa tcggctatta cagaagcatt
     3181 tgagaaattt gctttgcaaa ccggcaatct cttcctgacc gtctctgcca atctggagct
     3241 ggaattgtct gaactgccgc ctgatgatcg gaaacttctg atgaatgaat ggaaaattac
     3301 cgaaccgggc ttggatcgct taattcgtgc cggctatgaa ttgctccgcc tcattacttt
     3361 tttcactatg gagtcaaaaa tcgtccaagc ctggacaatt ccggtcaata ctccagccgt
     3421 caaagccgcc gccgaaattc ataccagctt tgaagaccgg tttatcaagg ctgaagtgcg
     3481 ccattggaaa gatatcgaaa gtctgcgcag tgaaaatctt ctccgcgaac agggattagt
     3541 gcatattgaa ggcaagtcct acctggttca cgacggcgat gtcattacct ttaaactcgc
     3601 cgccggctaa tatggcttcg ctggtttctc aattcgataa actcaatagt cagtataatt
     3661 taatcgccga caatcaaaaa atcctcattg ccgtatccgg agggattgat agtctggttc
     3721 taagccgcct tttattcgaa ttacgcctgg ctgagtttaa aaatctggaa ttattggcgg
     3781 cttatgttcg ccagaagtca gtggcgttga tcaatgaagc actcaaagaa attgccgatc
     3841 tcctagccga ctggcaaata ccattttcga tattagatag tgaattgcca gaatcgtctc
     3901 aaatcaattg ctacgcttgc gctcgcgaaa gacgaaaacg actattccag tttgcgataa
     3961 tgaacgattg taacgccata gcttttgggc ataatctcga cgattatctc gaaaccggtt
     4021 taatgaacct ggtattcaaa gggaatctcg aatcgctcca accggttcag atgcaactgg
     4081 gcgggcggat acgcatcgtt cggcctctgc ttaattggcc caaaaaacat atcagagcaa
     4141 gcggacaagc ttatggaatc aaatccatcg cacccgtttg ccctatggca agtttcaata
     4201 aacgtcaaga tataaatgca atgattacaa gttttagtcg cctgaatcga ggttttcgga
     4261 gcaatttacg acgcgccata aaccactgga atcagctctc aatctgaaag tgccttcttg
     4321 atgtagttag gctttaggcg gactttcgat cttgctttat tattcggaca agagtttata
     4381 ccgatcacct cggtctatcc ggatggcgag tgattaccaa ataattggat tctcagacta
     4441 atttaagccg accggcggta tagttcattt tggcgaatat actcagccac ggcgggcgga
     4501 acaaattgat ctatctgtcc gccattcatc gctatttggc gaatatcgga agaggaaaat
     4561 ggtattaggg gcgcattgaa aaataggact ttagccagat attccggttt gactagactt
     4621 agatcgcaac cggggcggcg ggcaacgacg acctgaactt cttgtaaaat atcttccggt
     4681 tgccgccatt tatgaaattc tatcaaacta tcggcgccca ccacgaaaaa taaatcttca
     4741 cgctcgagac gcatttggtg tttaatagcc cggatcgtat caatcgaata agatatcccg
     4801 ccgcgttcga tttcaattgt ggaaatctcg caagctggtt cgttttgtaa agccaacttc
     4861 agcatcataa gccgatgcac cggcgaggtg tgaatttctg aggttttagt aggcgagatg
     4921 ttagccggga taaaaatcaa tttgtttaga gagaaagctc ggatagcggc tcgggcaatt
     4981 tctaaatgac cgttatgcgg cgggtcgaac gttccgccga ataagccaac tttcaatctg
     5041 ctctcttgcg tttttctctc tctattttac gaagttcttt gtgataagaa gtgcgcagat
     5101 attcaagaga attcgggtcg gtttgggggg tagagcgggc aagaatttcc ttaaaaagaa
     5161 gttcgtactg atcgaattgg cgttgacgaa ttaggcaata ggctttctcc agtcgcgctt
     5221 tttcagccca ttcggtatca tagtaggttt ccagcacttt atcgaagtaa ataatcgccg
     5281 ctttccattc ctgcattttg cgataaagtt ttccactttc aaaaagcttg cgggctaatt
     5341 ttgagcgaat ctcggcaatt ttttgctctg aatcggcgcg gaaacgactt tggggatagg
     5401 tatcaataaa gtcctgaaaa ctttgcaagg cataaataga atattcttga tcaagctggt
     5461 accgcgggga agatttgtat tgtgaaagag cgattttata aaaggcttcc tcgacatatt
     5521 cgctgtaagc atagcggtta atcagttgct gatattccga agccgccagg agatattctt
     5581 tacgcttgaa ataacattcg gcaatataaa actgagcgtc gtccgccagt tcgctaccgg
     5641 gggaattgat aactaccaaa gtaaaatgct cgatcgcctt gtcccatttt ttcttattaa
     5701 aatattccgt tccttggcga taatgttgct cgaaatctac aacaggtttg ggtttgttag
     5761 aggcacagtt gtttaaataa ataatcagca agctaatcag gacaattttt aggatttgac
     5821 gtttgggagg catttcaatc cggcacagtt tcgggtaggt ttgatcggta aattatttta
     5881 gaaagccact gaaagagaca aaccacgaat tggcatttta taggtacaat cagtcaccaa
     5941 gccgacatgc cagctgacat ctttctgttc tggcggtcgg taatttttag cggcgatttg
     6001 agcatgaaag gcgctgatca ggtggttgaa cataatcacg gtcgtaaaat tggttgccat
     6061 tttcaacgat tggttggatt catagcgttg gttgagatac tgtttacgaa gagggctggt
     6121 gggaatattg agagaatcaa cctcagatac gatttggtca aaatcactcc aaccggaagc
     6181 aaactggtcg tacttaccga tattttcgta ataatcgcgg gttttgattg gtctgagggt
     6241 cccttcttgt aaatatgttt gccaaccagg gagttcacga tccagataag aaacatcggc
     6301 tttgtattct ttaccctcat attcgaaata gatagtatgg gaaccaaaac cgctaccgga
     6361 atcggaaagt ccaaagtatt cggggtagga gagactattt tcataccatt gccaggcatc
     6421 ccagtaagta tttgcccagt tcatgtattt acgttctaat ttttccccat tattcttgcg
     6481 gttgaaatag ccgacccagg agacgacttc tacactgaaa aaagctgccg cccggtactt
     6541 ggctccggca taaaattcgc ccgcgcccgg tacgaccgcc gataaaaaga aagccagtcc
     6601 gggtgatttc tcataattaa aaacgaaagt atcctcatca gcctgggcat atttcatact
     6661 atagattaaa cggtcacgct cggtcaccgt cgtgcgttca tcgaaaaacg actggccgaa
     6721 tcccatgatg gggattagaa ttgcgcaaag tagtgttgcg gctaaaaatt tcgacttcat
     6781 gtagcctccc ttataagcca aacaatagcg taaaataagt tcgccattca tgtccgtatt
     6841 ggtaagtttt agaattggaa accacctcaa aatcatccag cccataaaca aagtccaggc
     6901 tgattgaggt gggataggaa taaaacgaaa aaccactcat acgcacttga aaaccaatat
     6961 cttttttaaa atcatttaca atattacgta gcactgaaac aggctgggtc ttaaattgtt
     7021 cccatttcaa tcccggaaaa ccggttacct gattccaggc attaccagcc tcaatataag
     7081 tcgctacgaa ggcattttgc aaattgaatg tcaggatttg aaaatgtttc tcacgaaaaa
     7141 gtggcagacg ccaagtataa tgccaactgc ttagattgcg cccttcgata ctgtaaaatg
     7201 gataacctct cagtcccggt tcgccaccag caaagaaata gaaaaatgaa tcaacatccg
     7261 gcttagaaat ccagcccaaa tgccatctca aagtgccaac caaattttta aataagggat
     7321 atttgtgata ccaattgcct tcatgttcca atcgccagta ataattattg cgaaaatcta
     7381 cttgaatagt gctgtaactg ctattgactt taaaaccttg tataaatttg tcataattgc
     7441 gtgcaatata agtgtccagc agaatgccgt tatccggatt agtttcctca ttcactttcg
     7501 gtaacatcat ccgcatctgc caatgcagtt gaaaattaac gccgcgatag taatcatagg
     7561 aaaagccatt ttgataaata tcggcggctg tgatggtttc gtcactactg gtgcggtaac
     7621 gcgaccattt aacatcgaaa cgaacattat tgaaaccgcc aaaagggcgg gaaatgccgc
     7681 cataagcttc ggtcaaataa aaagtatagt ccgcttcgac tggccagcct ttatagattg
     7741 acttctcgtt aaagatattg cgactgatat tgtaaaattc caagaagata gtcggctccc
     7801 attggtgata ttccagaatc agaaaaagat cgcggtcttt gattctatta atcgaagccc
     7861 ccgcaaaaat attgaagcgg ttgattatct catttgactg acagtagaat cccggcttga
     7921 ccgttccata atcaatcatc aagcgcggca taatgaacat tttggcaaat tgatcgtgat
     7981 atttctgcgg ttctggaaga gacaccggtt gaatctccga aaccggtgga atattttgat
     8041 catagtctaa ataaactgcc aactgcggtt caatcggttc ggggttttta atacaagcaa
     8101 ttttaaatgc ccgatcgtcc agaatagcat aaaccaattc gcctttctca ttgaccgctg
     8161 gcataaaagc accgccggta acgtttgtca gtaattgccg ttcgccggta gttagtgatt
     8221 gccggtaaat attgtaaatc ccggtctcat ctgaggcaaa gtacaaccac ttgccatccg
     8281 ggctgaaata gggattgcgg gtatcataag ccgcctcatc caaaatctgc atcgctccgg
     8341 tttctacatc aaccaaagcc agattacggc catgaccaat cagatagtcg aaagcaatcg
     8401 tgcgcccgtc cggtgaccag tgcaaggtaa aaacctctac tccttcactg aaagtagtaa
     8461 tttctctgat tttattagag tccagattca ttagggctac attgctggtg ccatctcgac
     8521 cggtgacaaa agctattctt tttccatctg gcgaaagggt tggattgtag acccgagcgc
     8581 tcttggttag cggtttttcc ttacgtttct ttaaatcata gcgataaata tcaaaaaata
     8641 ccgagccgtg ccgattcggt ttactcgccc gggaataata taggttttgt ccgtcgggtg
     8701 cgaccgttac tctcgagtta attcgaggaa ctagcatccg actttttttc tgacgaacgt
     8761 catatacata caatccggtc tgagaataat aatctctccc tttattggac atgaaataga
     8821 cgattgaatc accgctccac tccggtccta attgcatcgt accctcagcc agaataattt
     8881 cgccgacttt ttcgttagcc cggataattt ctgtttgttt gaaatatctc tgttccaatt
     8941 cggtttgcca ctgttggtaa atctcatcag ccgcgacacc agtgacgttt ttcagagccg
     9001 tttcgcctga catccgcact ggcttggaaa gttcctccgc caggcgcgga atagtctcag
     9061 ttccgtattg ttctgtcaga taacgggtga aggcaaagcc ttgattatag accgattcgt
     9121 tgccggtgcc acgtttaccg aacgaaccca tttctgacaa agacaacaat gatttatgca
     9181 gaactcgatc gcgcagaagc atgtcacggt gggtgtccca gaaatcatta gaatttccag
     9241 ggaacatata ttgcgccata ccttccgcca gccacatcgg gattaccata ccggaaatcg
     9301 gataagaggc aatgatccga ggatagccat aaaccacatc ttcgcgcttc tccggttcat
     9361 catccagaac ttgaatataa attgccggaa ttgagcgccc gaatttcata gccttttgca
     9421 aggaaacaat gtgggtaaat tcgtgggtga tgacgtcgcg caaccagtcg tgattgcccc
     9481 gaaaaggaaa atcgagcggt gtagcccaga tttcgatttt attatcgtaa taatatgagg
     9541 cgccattcga aatatcatcg gtatctcgga ttaccagagc ggttttttca cttggttcaa
     9601 attgataaaa ttcagttatc ggtccataaa tagtttcagc aacttcagcc gttcgtcttg
     9661 cagtccactc cgccccttga tgataatgga ttttgaaatg agcggtctcg aaggataacc
     9721 actccagttc aggatgatta taagtcccga actgtgcccc ggctggatta aaaaaaaaga
     9781 gcattattag aaaaatgacc agcgtattcc ggcaaatctg gcgctcagac attattttac
     9841 caccagaatt ttaattacct tagaataggt ccgcgaactg gatttggctt gaactacggc
     9901 gaaataaact ccactttcga cattactgac gtcccattcc ttttcatttg gctcgccgat
     9961 taaaggcgcg gaatccttga aatcggcaat cagatcgcca gccaaatcgt aaatattaat
    10021 cgaaatatta caactgctat tagggaagta acgaatgtat gtacgattat ctttaggcgg
    10081 attgggccag caaaaagtcc tggcattatc gagcaatgac ttggactgaa ttggactgct
    10141 ctgggattgc accggataat agaattggcg agcggcattc ccaccgtaac aaatccaatc
    10201 ttcagaatca tgtgaaaaag aggatagccg cacagccttt atgcaattgt aattttgagg
    10261 atttgattcg gttgacgtca gtgctaaata agttactccg agtggcgtat ttaataatat
    10321 gccagacttt tggctctgct ggtaggcaat tttggggaaa ccgctcaata aatctccttt
    10381 ataattgaac gccaacaaag ctgaagacat attgataatg acatcctttt ttccgtcatt
    10441 atctaaatcg gctgtcaaga gttccgattc gaaccggtcg gaacctaaaa tattcggaac
    10501 cccgatcggg aaattgtttt taactgtcaa ttcctcgttg aatgccaaaa cctggctgga
    10561 agtggcagtg ataatttcga tttttccgtc gtcatctaaa tcggcacagg ctaaattgcc
    10621 gttaaattcc tgttcccggt gtaaaacaac ctcatttggg gtgccgatgt cgagaacaag
    10681 agttagcaat gaatcggcac aatgaattaa atccacactt tcattttgat tgataaaagc
    10741 tattaccatt gttccagata aggcgttcgg caaatcggct acgacttcgg cagaaagtga
    10801 attaagatga atcctgatta cctgattatc atccgttatt ccgaagatat tatcgtcgtc
    10861 accgaccagc attttaacct gtgaagcgag ctccaatgac tcaatatttt gagtatcaat
    10921 ctgatagagg tgaaggtaat tttggttgtc ggcataatct tgggtagcga atagcacacc
    10981 tccctttact ggtagaagag ggctgaccaa atcctgagaa ttcagactga cggtatcagt
    11041 tttattaatc tggccatttt gcccaaattt ccagatagat aaactcaaag aaggcgcctc
    11101 gggattattc tgaacaattc cataaagtaa taagctatct ttatccagtg cgatcgctgt
    11161 ttggcgcggc agggaggcta tttgttcgcc gaaggaagta tgaatcctta tgctgtcggc
    11221 taaaatcaga aattcttcaa cctggtcatt tggattcaga tctagcggta atatttcgtc
    11281 ggctcccgca ctcaggctaa gcggaaaatt tgggagcgca tcattcagtc taattcggaa
    11341 ggttattact tcgccggctg gaccgatgct atcaatacta atgccggtaa aagtataatt
    11401 attactatac gtcgccggat tagtataagg cgtaaagccg acatgttcct ccacatctaa
    11461 aatataatcc gaattaagat caataaagcc atcatttccg gcaaaccagg gatcgaaaaa
    11521 ccatccatcc tcgatatctt cattcaaaat atcggcttca tatcccaaat cctgggcacc
    11581 atcgccttct tccatatcaa caccccgatg ttctcggtca ccgttaacgc tattagtcgc
    11641 taaattggcg ttaatgaccg attcatcaat gtgccagatt aataaaccgg agccttctaa
    11701 accataatca taatggggca ctgctaacat cacgccggtt tccggtgaaa attcggcttt
    11761 tagtgagtca cgaatcaagg gaaaactcag cggcaattca accatgccgt tttccatata
    11821 aatacgatac tgaagactat caaaactgac gcccgggcga aaatgagcgc accgattttc
    11881 tactaaaaaa tattcgttag cattgattgg gatcttccaa acactcgtat cggaagcaga
    11941 accgactttt ttaaggacaa catcttcaaa atttttagcg actactggtg tttcccaacc
    12001 gagaaaaacc cgttcccagg cacacggcac cgctgggact agcccactca aattagccga
    12061 accttgatcc attaaaccaa attttccgat gccggtcatc ccgctttccg tgtcaaataa
    12121 acagggcaag ccaagataaa aaccaaacat aaatgccagg gtgccattca aaccgaattg
    12181 gtaatgacag ggggaatcgc ttccaccgaa tacctcttcc caattatcat atagcaagtg
    12241 attctggctt tccggtaaga ttaagccgca atcaacgaaa gccgttccgt tttctaccgg
    12301 aataccctga aagtcggaat cgttcggtgc taaaaatgcc tttagatcat ccttgttcat
    12361 aaaagctgat agaatgtcat aaggcgtcgg atcaagcgta atggcaaaat cctgaccaac
    12421 tccggcatga aaaactatta cgacatcgta ttgcgcaaaa tccacctccg gatcggcttt
    12481 ctggactgac tcaactaaaa gttcagtcaa gcgcaaatcc accgaatctt cgaccaaaaa
    12541 cgggtggtaa taagccatcg gatggtcaac ctgataactg ttattatcat tcagcggaaa
    12601 aacttccgaa ttagtcgtgt ctatcacaac tttgccccgg gaaacccgat tataataatt
    12661 tgccaaagag cgaatatgat cccgaaaata agcccgattg tgaggcgccg gatcaactac
    12721 gaattcgtca tcacagggag tctctacggc ggacattagg aattgcccag tgccggtggt
    12781 ggtttcgtct tcatcagccg ggaagctgac gcgcagggcg caaatgcgaa tagctgcagg
    12841 cgcagtattt ttcacaaccg atggagcagt cgcaatccga tactggggtt caaacctacc
    12901 gtatgcggct ttattagcta aaacaattag tgttatcccg gtaatcaaat atttatatat
    12961 tttcatcgtg ttaggttttt agcctttttt aaatagggta agaactgttc tgattatcta
    13021 aaattaaaat tttatattca tcgagaataa cattgtattt tgacgaggat gtccgggatc
    13081 accggcggta tagccgaaat cgaatcccat acccgcccag cgcaaaccag ccccgaaagt
    13141 cggtactggc catttacctt tcatcgtgat attgccaggc ttatcataca tacaaccagc
    13201 ccgcagagca aacaaattgg agtaccaata ttctattccg aagttatacg tcaatttttc
    13261 gaattctgcc gcaagcgagc cgtcattggt attaccatcc ttatccgcag aaccgatgcc
    13321 atccgtggcg gaatcataca ttaataacca atcgtcaacc caggaagtaa aaatccctgt
    13381 ataccactca tctgaatggg catattcacg ctgacccttt ttattatatt caccaccagg
    13441 cacagcatga ccattttcat catagccacc aactttttca tcgccgtcaa tatcccgatc
    13501 cggataagcg gctaccaata ttttgttaat atcaaatgca attgtgactt tgttgtattc
    13561 attacctaac ttatatgcca gaccaagttt catattagta ggcatcgggt cggcctgatt
    13621 aacatcaatg aaggcaattt taggcccgat gttagccata ttgattccca gagtaagatt
    13681 tttggttaga aaactatgtt ttaaataagc gatatcgaag cagaaatttg ttgagtagcc
    13741 ttttcctaat tcttttccgg cgccttggtc ggtcaggtgc tggtgaacca ttttcacacc
    13801 cactccgatc gaagacgtgc gcgtaagagc ggtcccatac gaacctgtta cagccagcat
    13861 aaaggagttg aaggtgccga gatcatgtcc gaattcatcc gtacgttgct gttcaccgag
    13921 attcaacagt ataatgtttc cgcctactgt gccaacattt ctgataaatt gacgatatcc
    13981 gataaattca taataaatat cactaactaa gttcggtaac cagttgacat gggtcaatgc
    14041 caattcccga ccgcgcagga atcccaatcc cgccggattc cagtagctgg cataggcatc
    14101 gtcggcaaga gccgctccca cttcacccat tccagaaaag cgtgcccctg gattaatcaa
    14161 taaccaaacg gcaccggctt cgccttgagc caaggcgccc gccgtcatta atgatataat
    14221 cccgagcatg gcgattattc ttttcatcaa aacctcctaa gaacaaaaaa ccgaacatca
    14281 atactttcgg tctgaataat atacacaaac gagtaatttt aacgaagatt atttatctat
    14341 gacttcgaaa actggattta gcaataaagc cgcgaattaa ctcctgataa attttaaaat
    14401 ttttcagcct cttcaattaa cataatcggt atatcgtctt taatggcata tttcaaacga
    14461 caaacgttgc aaattaattg ctggtttgaa atatcgtact caagttcgcc cttgcattgc
    14521 gggcaggcta aaatttcaag caattcatta gataacatat atttctcgaa aatttttcat
    14581 tgttttcaac gacgacagtt ttatcaaact caccaatact gtcaattgtt ccttttttat
    14641 atacaatcag tttcctccaa atttattaat atgatcttta aattcatcca aatccttaaa
    14701 ttttttatag accgatgcaa atcgaatgta tgccacctca tccaattcct taagcttttt
    14761 catcactaat tcaccgatct gacttgaagg cacttcgcta ccggaagttt cttcgatttc
    14821 cttttcaatg tcaattacca gagtttctag cttctcagca ggaatcggcc gcttgttaca
    14881 ggctatttta atactattta aaagcttatt gcgatcatat tcttcacggc gaccatcatt
    14941 tttaatgatt tgaagcggat attctacgac atattcataa gtcgtatagc gatgcccaca
    15001 gtcaatacat tcacgccgcc gcctaatggc attgtttttt tgcacggtgc gagaatcaat
    15061 cactttactt tccggagaac tacaaaaagg acaacgcata actcacctat aattttatct
    15121 cttgatagag tgggaaattg ccacatagtt cgccaacctc tgaacgaact ttctggtgaa
    15181 tatctttgga atcaggattg cttaaaacgt ggtcaattaa tcctgcgatc gtacgcatct
    15241 ccgcttctcg cattccacgc gtggttaacg cggctgtacc aatgcgaatg ccgctggtga
    15301 cagacgggct cttctgatcg aagggcacca tatttttatt ggtggtgatt ccggattctt
    15361 ccaggatatt ctcagcctgt ttaccggtaa tatttttatt gctcagatca atcaatatca
    15421 agtgattatc agttccaccc gaaaccagat tgtaatcgcg gtggagaagt tcttccgcca
    15481 ggacttgagc gttgacgata atttgttggg cataagcttt gaattccgga cggagagctt
    15541 cgccgaacgc cacggcttta gccgcaatga tgtgcatcag cggtccgccc tgaataccag
    15601 gcataacgtt ggaatctatg atttccgaca tcattttggt gcgtccgctt ttgggagcta
    15661 ccagaccgaa tggattttcc cagtctttgc ccatcaagat taagccgccg cgcggcccac
    15721 gtagagtttt atgggtcgtc gaagtaacaa tgtggcaata tggcagggga tcgggatgta
    15781 atcccgcggc gatcagaccg gctggatgag cgacatctgc taccagaaaa gcgcccacac
    15841 tatcagcgat ttctctaaat ttggcaaaat ccagaaatcg aggataagcg cttcccccgg
    15901 cgataatcat cttcgggcga tactcaagag ctaattccgc cagacgatca aaatcaatgc
    15961 gaccggtgtc tttttttact ccataggaaa tgattctgaa aagtttcccg gaaaaattta
    16021 cgggactgcc gtgcgttaaa tgtccgccat gagctaagtc cattcccaga acggtatcac
    16081 cgacatttac cagcgaaaaa tatacagcca tattagcctg cgaaccggaa tgcggttgaa
    16141 cattggcata atcagccccg aagagttttt taactcggtc acgagctaaa ttctcggctt
    16201 ggtccaccgc cgcacatccg ccgtaatagc gctttcccgg atagccttcg gcatatttat
    16261 tggtcaaaac tgagccggcg gcttccagaa cggctggact gacaaagttc tccgatgcaa
    16321 tcatttccag agtttgtcgt tcgcgctcga tttcggcttt aatcgtatct aaaagaacgg
    16381 catcctgttt agccaggaaa ttgtattcca agttatcaac tctccaattt cataattttt
    16441 tcaacacggc gtaggtggcg taaatcatcg gaagatggcg tttctaaaaa tgtcttcaat
    16501 atttctttag ctgattcaac tgtagtaaaa tcagccccga aacagagcac attggcgtgg
    16561 ttatgcaggc gactaagtcg agcgatttca ggttggtaag ccattacggc tcggataccg
    16621 tgaattttat tagccgagat acacataccg gcgccagtcc gacaaataag cacaccaaat
    16681 tgggcgcggc cagagacgat tgcctcacca accgcccggg cataatccgg gtagtcggca
    16741 ctgtcatcgc tgttgacgcc gaagtcaatc acttgatagc cttctgaacg cgcgaattcg
    16801 attaatgcgt ttttcaggcg atagccacca tgatccgctc cgaatgcaat taccatattt
    16861 agttcttctt cggttcgacc gtgatccatt tatattcggg gctaagaggt cggaagctgg
    16921 tacgcattgc cccgttagca tcataacggt taaagaacca ttcttcagga gttggaatca
    16981 gatattcttt aaggtggtca acccgcgctt tgacgctggt gtcgcctaat tccattactt
    17041 ttaaataatc gtcataggct aatttataga ctaatttatc ctcaaaagtt atttgacgtc
    17101 cgctcgtata ataatcagcg gcggcaaaat agaggtcggc tctaattttg taagcttggg
    17161 gcgaattggg tgcaattgtg atggcttttc gggcatattc cattcccttt gggaaatcac
    17221 ccaataattg acagcatcgg gcggcttgtt ggagaatatt taaatcatta ggagaaatat
    17281 tccttaagat ttgatcatag gctttcaagg cttcagcata attatttttc tgagtgaaat
    17341 aaatatcagc gatattgctc caggcttcaa tatttttagc gtctaattcg gcaacctttt
    17401 gatatacttc gagggcttta tcgtattcgc gctgttgttt aagagcggcg gcatactcca
    17461 agccatagcg gatattggta ggattctttt cccattgttc tttctgaatt gtagtaatgt
    17521 ctctaccgag cttctggtag gcaaaaattc ggtcgttaag ggcttgctca tcattaggat
    17581 tgatctttaa aattccgtta gtagcataga tgacgtcttc gtagcgctgg gcactgatat
    17641 acagtttgac taaagtgcga tagagctcaa tatcatccgg cgctatttcg atcatttttt
    17701 cgtattcccg aatttctaag tcaattttac cctgcaggtg gtagatataa gcgatatttt
    17761 tctttaagaa aaggtcattt ggcaaatatt tttcacctgc taaataaaca tatagggcgc
    17821 tatcgagata aatgctattt tcactggcta attgctggta agctctcccc caaaagggaa
    17881 aaagatcctt agcatattct tcttcacaac caaaactgct cattttctta taattttcaa
    17941 ttgcgcttct ccagttttga ttttgataat aactgtaagc aaaactcaaa tacaggtcgc
    18001 acttatcccg atcaatggtc ggcttggttt caacttgctc cataaccgat tcgctcgatt
    18061 ccggtgaagg aggagtgcag ttcattaatc cgatgacaag gcaaacgatt agtgcttcct
    18121 caataaaatg aatccatcgt ttcatataat cacctcattt attttattgc tttttatatc
    18181 ttctaaacca gggttcaccg gttgtaatcc ccaggtgaat ccccagtatt ttttcattgc
    18241 cgatttcgtc tgataaaaag ccattcttca agccgtaagt taatgcgaaa tctattcgat
    18301 tcaaattata aaaaaatggt aaactgatcc cagccgtgac gccatattca taaatcccag
    18361 aattttgata gaccggttcg gtttgataat aagccccaca gcggtaatat aatttttgga
    18421 aaaaggtgct cttcaattcc tgacgtggtg atctttctaa gccgattccg acagcataag
    18481 aattacgact gtcgctttta aactgttgaa atatgtagaa attttggaat agatcttcct
    18541 catatatacc gtattgcata tcggcattta aggctagatt tctagcgatc ggcaaattaa
    18601 ttccaacctt tataattgtc ggaaaattaa taggatcgga ttttatagtg gtgtcgggac
    18661 caaagttatt ttcgatgatt gtccaccttt ttaactcgtt ccctttttcg accgtcgcag
    18721 ccagattata ccgttgactg cgatcactta acaaaaggcc gaagctgatg cggctaccgt
    18781 caacggctat agtgcgttta tagtgacttt ctgagtcaat atcggaatca atcttggtgt
    18841 gattacggac cacgtaatta ccgaacaaaa aattagctct caagccaatt gccagatttg
    18901 gagaaacccg gtagccgcta ccaaggtata actccgaaat tccccctgac aattcaacta
    18961 cggaattata ttcgaattta tcgtcttgaa atatcaaaga atcagcaaaa ctaaccgaag
    19021 atttcattct ggataagggc gccagaccaa ttgcgaggcc agcacgtttg ccgaccggca
    19081 atttaaaaga aaagcccgaa aaatttacta ttctaccttt gaaatcaagg tcaggaattg
    19141 agagagccga ggtgtacatc tggccttgta aggaagtact gccgaatcct tgccagagcg
    19201 ctggattaga ttgattcaga ctcatcgagt ctctaacggc tgtccctatc cgacccattc
    19261 ctaatattcg cacggaattg gctggcgatt caactccgaa accgtagttg gcataataag
    19321 aaatttgcga ccagccattt agactgagaa tcagtaggaa taaaatagta cgacgagatg
    19381 ttttcattct tcttgcatcg ttaatatttc caaacgaggc cgccgcgccg gatcgggatc
    19441 ttcagtcgga taaaatccca gcatagccgg gtaaagccca cttggcgaaa tccataaggc
    19501 taccccgaaa ttactgatat tgccactggt aaaaagttgt accgtactgg gaattttcaa
    19561 ggtcaatata ctatcgctgg ccgaaaaagt ttgtgaagta acataggatg aacccgatgg
    19621 tgaaaacgat ccactttccc actccgctga gtccaacaaa gtaatataaa ttgtgtgcga
    19681 atttccgtaa tcttcggtta gctccgaatt aacatgcaag taaagataag caccagcgat
    19741 aaataaattt ttatccgtta tgagttcctc tacctgaaat ttaaggaaag tataggcggc
    19801 ttttccggca ctgattagca agggctgagg cggtgcggtg aaggtctttt taaattgcgt
    19861 aatagttaca tccccgacac aggcaagagc tgtactgata gtatctccat tatcagttat
    19921 atacgaaatt atgatatggg gagaggaatt agtttctccg gaataaatta ttcccagacc
    19981 ggcagctgaa ccgaccgccg gtttaattac gatgccataa tgggattgag ttgagtcgcg
    20041 ccagttggcg ataaaagatg tggtcaaatc gaggattaaa gtatcagcat ccgagtaggt
    20101 aaaagttttc caatcggtta gatgataatc atcaaggctg aaattggtac tgttagtact
    20161 atcttctgac caatccgcat tctcctcacc ttcgatagaa gcgacggtca tttctaaaga
    20221 cgggtgaaca gcggtgtcac tcgaaagatc actgccggtc attaaaacca attcacacga
    20281 gaggatttta gcaaggggaa gggtatcaag cggtgttggc aaagttttaa acgataaaag
    20341 gctataagct gtaagattag cgtcagttcc taccactaaa taggagctat agccgatatt
    20401 aggttcggca aaaacggtcg tatccacaat aaattcagca cttacgctaa ctgaatcgaa
    20461 actgcataag tttttatcta atggcagagt tggtattttc tcggaacaac cgaacaacaa
    20521 aaggcaaaag aatgccgcca cactccggcg ataatcaatt tttacataat gtaactttga
    20581 ccagacacgg ttgaaatatg gtttagagct gttccataat attgatgatt tcatctgcca
    20641 atttttgcca atcttcaggg gattcaccat ttaactcgta agaagagaat ttcttcttat
    20701 cctgcttcat aatttcgctg aaaataggat ctttctgata tttttccagc aaattttcat
    20761 ttttattaat gattaataca tgatcacaat agggcatagc cgcggctact acgtctaatc
    20821 gcccatctac gctttttgat aaatcaagat tgttcaatcc tattttttgg tattctgtta
    20881 ctttaaaatt agaacagtca actttggaat ggatgatttg caggatttta ataccttcgc
    20941 aatactcttt atcaaccaaa ttagactgga taaaaagagg gatgagactc atttgccagt
    21001 cattgcagat aattacttcc ggtttccagc gcaaatagct cagggtttta agcgccgcca
    21061 tggcaaaata gccaaatcgt acggagttaa gatattcatc cgagacacat tcagtcgggt
    21121 agagattttt atcggaatta ttaaaataat aaggatgttc cagaaaatac acctgaactt
    21181 tggtattggg gataaaagcc gatttaaccg aggctttgat attttcacca cggtactgga
    21241 ggtccatttc acgtaagcga atcacttccc gtaaaatata gcgtctttca ctaatgaagt
    21301 tatatttagg catcatcaag cgaaagtcgt atttccgctc gttgaaaacg attgggattt
    21361 ccttggaaaa aatcgccagt tgataggttt cggcaaatgg gattatctca gaggttaaaa
    21421 agtatattct cttaatcata agtgttttta tttaattaat tgtttggcgc gttctgaata
    21481 ttcactggtc ggatacagat taatcaattt ttccaattca tcttttgctc gtttctggtc
    21541 gcccatttgt ttaaaacata gtccaatttt gtattgggca tcgtcatctt tatcggtatt
    21601 agtaaaattc cggacttttt caaattcaat gatagcctgc tgatattgtt tcatcgaata
    21661 gtaactttct ccgatccaat attgggcatt atcagccagc tcattggtcg gatcatcggc
    21721 gattaatttg cgaaataggg cgatggcttc ttcaaattta cctttctggt gcaaactcaa
    21781 ggcgttcaaa taccgatctt tataggtgat gccgggcgaa gtaatagagc gatttgttgc
    21841 ttggcgagca tctgtctgac tggctgaatt gtaatcactg atactattag acaacgatgc
    21901 taaacgattt tccagaataa ccagctgaga tagaatttca aaattgatac tatcggcgta
    21961 gcgtgttctt tgttcgacag tattcaaacg tttctgcata ttggcttgtt cagcggaaag
    22021 aatattgagc ctgttttcaa ggttagccat agtcagttga agcggttcga tcaatttatt
    22081 gatgtcagtg gttagggtag ctttagttat agactctttg tctttggcgg tcagtgaatt
    22141 gactgttttg gttagatttg cgatttgggt ctttaaatcg gaaatatcca ttgctaatcg
    22201 gtcgagactt gcggctattt cggcattact gagccttttg gagtccgaac gagccaaatc
    22261 aagatttatt accattaggg ataatgcaag aacaaaaagt ttaatggcag atggcttaaa
    22321 ttttaggtta tttttgattg gttgttgcat attattaatg acataaattg tgacgaaagc
    22381 tacgaaattt ctcggtaaaa agaaaaattt attttccaat cagcttttga aaatttaata
    22441 ttgtttatct actccaatgt tgagcgccaa tcctaccaga ataaaacagg tcatcaaaaa
    22501 agatccacca taactaataa atggaagtgg tagtccggta actggcatca gtccaacagt
    22561 cattgctaca tttatgacca catgtatgcc gattatgctg gcaatcccaa tcatacaaag
    22621 tgaacaaaag cgatcttttt gccgataagc aatattaatg caaatcagaa tgaaagctaa
    22681 aaagagtagt aataccacta acaccccgat aaacccatat tcttctccaa ttacagaaaa
    22741 tatgaaatca gtatgctgtt ccggtaaaaa tttaaggtgg gtctgtgtcc ctcgagctat
    22801 cccttttcca aaaagaccgc cggaaccgat tgcaatcttg gattgaagaa cctggtagcc
    22861 ggtaccttgc ggatcggcat tgacattgaa tagtgtcaaa atcctctgtt gctgataagg
    22921 ctttaaacta ttccataaaa acggagtggt aaaaccaagc gatagattta tcaacataac
    22981 cgttaaagaa accctgagtt tttctttggt caagtacaaa atgataacta cgataattac
    23041 ccagataaaa aaagtataaa aattaaaggc ggttatgatg cttaaaatcg gggcaatgat
    23101 aataaaaaga tggaataaac gggcgcctgc ccagtacagc atcggaaaca acatcccgaa
    23161 gtaaataatt gatgttccta aatccggttg tttaagcacg acagccattg gtaataaaga
    23221 gagtagagta ggaatcaaca caaatttaaa atccaagttg gaagatgatg tgctggctaa
    23281 gaaacgagca atcgccagca taaccattat tttcataaac tctgatggct gaaaaccggc
    23341 tgaaccaaaa gtaatccagc gggctgtccc agccaccgag ctacccagaa aatagggtaa
    23401 caacaggagg caaaaaccta acaaatacaa aatataagct gagtcgtaaa tcaacttttt
    23461 ctgtaaaaaa taaatagttg cggcaaccag accactcaat actaaccaga tcaactgtcg
    23521 tccgataatt ttctcaaaaa aggaaccttc catatttaat gaggcactat aaattgatag
    23581 cattccgaac aaacacaata atccaacaat tactaaaatt gccaggttca agttggagaa
    23641 ggagtttaaa aaattgttta ttcttttaga aattgagcgg cgcattgttt gctaaggact
    23701 gcctcttttt ttgttggtga ataaaattaa aaatctgtcc ggcaatctga gcggcgattc
    23761 cgccgcctga tttaccatgt tccaccaaaa ctgcggttgc gatggtttga ttttgttcct
    23821 tcatatatcc gataaaccag gcatgggcat cgccgtgtgg gttctgggcg gtaccggttt
    23881 ttccccaaac tgggacatct ttgacccgcg cggctcgtcc ggtgccgcgg ttaatgacat
    23941 cagccattgc cttctcaatg aattcccagg tgtccacaga aatattttga atcgagtctt
    24001 cggcggcttg aaaagtgatt gtctcattgc tccctcggtc gatatatttt aatccaacat
    24061 gcggttgcac caatttacca cgcgacgcta aagcggcaca gaagcgagcc atctgaactg
    24121 gcgttaccag aatatcaccc tgtccgatta cgaggtttaa tttattgccg tctgtccagc
    24181 catgttcgcc gtatttgcga ttcagaaaat ctacatcggg catcactccc gctgattcgc
    24241 cgtacaaatc aaccccggtc tcggacccaa acttaaaatc ctttcccata tttgcccaat
    24301 cattcaaatc cagccgtcta atcaaattgt agaaataaac attacaggaa tggacaatgg
    24361 cttcgtacat attcattgat ccatgtcctc gctcttgcca gcatttaaaa atgcggtgcc
    24421 ccagacgata ctgaccataa catcgcaccg accaatcggg atcaaccgct ttggtttcca
    24481 gagcggtcaa aactgcgatt agcttaaagg tcgagccggg cgagtatgtt ccttgagtag
    24541 ctcgattaaa cattggtagg tcaggatcgc ttatcaatgc attccaagag ctatttgaaa
    24601 ttacaccggc gaataacgcc gggtcgtagt ccggtttact gacaatcgcc agtatctctc
    24661 cattatcggc gtccaacact acaatacctg ctttatatcc ttcagtcaac gactcaatgt
    24721 agatttgcag gtcaagatct atagccaaaa acatttcatt tcccggaatt ggaggaatat
    24781 tatctcgatc tactacctga ccgatttccc gtcctaaagc atccacctga atgaaattat
    24841 acccacgagt gcctttcaaa atatgttcat actgacgttc gattcccttc cacccaatca
    24901 agtcaccttg tttatattcc ttatattttt ctcgtattaa atcattggca gagatttccc
    24961 ggacataccc gagtgactga gacatttgaa ctccggacgg ataggaacgc accggtttta
    25021 ccgaataaac aacacccggc aaatcaaggc ggtgttcttc aatattagta attatttcga
    25081 cattaacatc ctcagctatt ttaattggga caaactgacc ccgccagtta ttttttatgc
    25141 gagaatttat ttctgcgaca tcgagcccga ggatttttgc cagaattgaa tccgttcgag
    25201 ccgtatgact gaattcataa gggataacat cgatatcata ttgtgattta ttgtcaacga
    25261 ttatttttcc attacggtca taaattatac ctcgcggagc ttcaattgga ataacccgaa
    25321 cccgattagc ttcggcaata cctttataac ttgggccttt gataatttgt aaataatata
    25381 aacgaccgat caaaaccaga aaaactagca caaagaaaat cgtcaaaata tttttcctga
    25441 tgttagtaat tgagttgtgg gaaatataca tactttattc cagagaaaat ggagcgaaat
    25501 attggattat gaaataaaaa actcccgtat ataaagtaga aggtaaaata tatcgcaaaa
    25561 atatagtctg ggtcgaaagc tctgctccct tgaagtttat cagatagata attgaaaaat
    25621 gaattataat aatcagtacc tcgatggcat aaaacaaaat gggatttaaa tgactgcggc
    25681 gtttcttaag caagccagct ccaaaaccgg cgatggtctt ggctaaagca gataaaccca
    25741 gaaatgagcc caatccgagt gcatcagcga ttagaccgat caaaaatcca tagatttgac
    25801 cagcaacttt tccttcacta cgaccaacaa acaataccag tattagcaga aaatctggtc
    25861 gaatcgcgcg gattgtcatc cagggtccga gcagtatctg gattattata gtcaccagac
    25921 tgaaagcaat tatcaggaga atctttcgta acattattta ctcaaaacca caaatacttc
    25981 ttcaatcgaa ttgatattca cacttggtcg cacagtcgca atctgaaaaa gattattggc
    26041 tgattgatgg acctctacaa cttcaccaac aattatattg ggcgggtaaa tttcgctgaa
    26101 cccggaagtc aatacttttt cgccaggata gataatagcc gttttcggta tctcgcggac
    26161 ttcggctaaa ccatttggtc gccagcgcat aatcccatgt tcccgcgaaa tctgaaattt
    26221 tactcccaac cgaaaattca catccagaaa aatctgcacc agggtattat tatcacccac
    26281 cgcaactgtt ttacctacga ctccatcgtt agtaatgacc gccatattcg gaccaacacc
    26341 atgcttactt ccgacattga tcataatcga attgtaaagc ggtgttgtcc cctgactgat
    26401 cacttccgcc ggcaaaattg tgagtcgaga cgtatcggca aaattcatca tttcccgcaa
    26461 gcgttgattt tccagataag cctctttcaa ttgggcatta agcaacgcca gccgcagatt
    26521 ttcttccttt agttccttat tttcttcgac aagattggac aattcgttga tccaaacctt
    26581 tggataatgc aaaaaagcaa atgcgtcggc tagctctccc tgagtcgctc taatttgagg
    26641 actgttgtta tgagccaaga taaataatga gagaaaaatt aaaaatatta gcagtaaata
    26701 ttcgtaatga tcaattataa aacgaattat tttcagccac atggcattta gactaataca
    26761 tcccgatatt tttccggatc ttcaataatc ataccagccc ctttgacaac ggagtgtaga
    26821 ggcgtttcgg ctacgttgac cggcaagtcg gtctcgaccc gaatacgttg atcaagtccc
    26881 tttaggagcg cgccaccgcc agtcaaaata ataccacgat cgagaatgtc agatgaaagt
    26941 tccggaggtg tattttctaa agcctgttta atagcttgaa taatcagatc gagtgtgtct
    27001 tttagacatt cacggatttc gactgaagaa acctcgatag tcttgggaat accggctacg
    27061 agactgcggc ctttgacctg gatagtcgtg tcattgatcg gcatagcgga gccgacggag
    27121 catttgatat attcggcggt gcgttcaccg attaacagat tatgctcggt tttaaaatac
    27181 tgcacgatgg cttcatccat ttcatcaccg gctacgcgga ttgcctgttt ggtgacaatc
    27241 ccattcagag cgataacggc gatttcggtg gtaccgccgc caatatcgac aattatattt
    27301 ccgaccggtt tggatatgtc aatcccgatc ccgatggcgg ccgccacagg ttcatctacc
    27361 aaatagattt cccgcgcatt agcccgatcg gctgaatcac gcaccgctcg tttttcgact
    27421 tcagtaatgc cgcttggtat acaaataacc attcgcggac gagcaatccg gctaagatta
    27481 atcttgcgaa tgaaaccgcg aatcatcgca tcggtcattt caaaatcggc tattaccccg
    27541 tctttcattg gccgaatagt cataatatcg tggtgggttt tgccgagcat atccttggca
    27601 tccgcgccca ccgccaatac ttcaccatcg tgagtcgaac gagcaacgat tgacggctca
    27661 tccaagagga taccttttcc tttcatatag ataattgtat tagcggtacc taagtcaata
    27721 gctatatcgc cagtaaaaat gttgaataag tttaatccca ttttcatttg ctacccttta
    27781 atgtttaaaa actcgcttac ctgtaaatat cataaataat ttacgttcgt tgcaagccgt
    27841 gatactttcc tgatcccgaa tactgccacc cggttgaatg aaaccaatta cttcttgttc
    27901 agccgcaata tccaccgaat cccggaaagg aaaataagcg tcggaagcca ttactgtatt
    27961 tttaattgac aaaccggctt cgccagcctt gcgaatagca attttaaccg aatcaactcg
    28021 cgacgtctga ccagcgccaa ttcccaccgt accatttgca tcgcagaaga caatggcatt
    28081 ggatttcaag tgctttacaa cgtgccaagc catacgcaca gtcggcataa ggcttggatc
    28141 gggttggctt tctgtcacaa ttttccattg actctcatca tcctcggcgg cttgaatttc
    28201 ctgaaccaga aggccttgac cataagaccg taaatccaaa tcatagtgca acgcttcggc
    28261 gtttggcatc agtaatcgca aatttttacg cttgcttaaa atttctaatg cttgattcga
    28321 atagctaggt gcaataatac attccagaaa actcttagta aattcctcag ccagattggc
    28381 atcaatttcc cgattcaccc cgacgatacc tccaaagtaa cttaccggat cggtctgaac
    28441 tgcccgccga taagcttcca gcgtagtatc tccgattgcg aaaccacacg ggttagtatg
    28501 tttgactatg acaacagcgg ctcgctcagt tttgaaagta gcataacatt tgtatgccgc
    28561 caggcaatca acataattat tgaacgatat ttccttaccc tgtaattgct ggaaaggctg
    28621 ccagttcgga ttattgagtg gtgcataaag agccgcactc tgatcaggat tctcgccata
    28681 acgtaaagtc tgttgcttat gaaatgcctg aacaatgact tccggcattt tttcagctcc
    28741 ggcgcccgtt aaaaaattgg agatttctga gtcataggtc atcgttttta aaaaaacagc
    28801 cgcggctaat ttttgccgat attccagagt caattcatcc tggtcgagcc gttgaagaaa
    28861 ttcatcatat tgtccgggat cagaaagaat gacgacattc ttaaaatttt tggatgccgc
    28921 ccgtaacata ctaggtccgc cgatgtcaat catctcgatg atttcctcaa aacttttatt
    28981 gccggaatga taagtttcgc tgaacgggta gagattaacc acgactaatt gaaatggctc
    29041 aaccttgacg cgcgccaaat cagcgcggtg actttcatca gtcgcatccg ccaacaatcc
    29101 ggcaaaaaca gccggatgta gagtcttgac ccgcccgtcg agaatttccg ggaaaccggt
    29161 taaatcttca attttttcca ctgcgatacc ggcagtttgc aaagctctat aagttccacc
    29221 ggtcgaaaat atttgaacgc ctttccgagc taaagcctga gctaaattcc caatattata
    29281 cttgtcatag acactgataa ttgcgcgctt aattttgaac tcactcattt ttcccaaaca
    29341 acctttccat ttacaaattt tattttatct tcgcacaggg cttttaccac ttccggataa
    29401 aggcgatgct cgacttccag aacgcgagcg gctaaacttt ccggcgtgtc gtcaggcaaa
    29461 accgctactt tttcctggca aatgacggac cctgtatcat atctctcatc aacaaagtga
    29521 atcgttaccc cgctaaactt gacaccggaa gcaattactg cctcatgtac cttcattcca
    29581 taaaatcctt ttcccccgaa atttggtaaa agtgcgggat gaatgttgac aattgcccgt
    29641 ggatatttcc ttacaaccgg gctcggaatc aacttcaagt atccagctaa aacgatcaga
    29701 tcaacgtcgt atttttctaa cacgttcaat aaggcattgg catactcttc cggcgtcgca
    29761 aattgtttac gattaaggca gaaattcgga attcccgccg cattcgctat ctcaaaaacc
    29821 ggaggtcgtt cgttatcact tataacgcag acgactcggg cggctatctc gcccttttga
    29881 attttatcaa ggatagcggc aaaattagaa ccgcgcccgg acgcaaaaac agcaatattt
    29941 ttcaaaacaa ccacatttaa ataaacattc aaatttatct aaatatcgtc gtagaatcaa
    30001 ttaagattta tctgataaaa aataaatctt taaatcgggc ttcagccggt cattatatct
    30061 atcgcaaatt gactggatca atatcgtcca tatcgtcgaa atcctgattt tctccgccca
    30121 ttccccagat aaaagtataa tgggcggtcc caacaccagc atggattgac cagctcggtg
    30181 ataatacgac ttgtttatta tgcatcacga tatgacgggt agattccggc tcaccgcaaa
    30241 gatgaaaaac gaggccattt tttggtagat caaaatataa ataaatttcg gtgcggcgtt
    30301 cgtgagtgtg aggcggcata gtattccaga cactgccttc cgccagttcg gtaagtccca
    30361 taactatttg acaggtatca agcactcccg gacgcaaata ttgaaaaatg gtacgcttgt
    30421 tacattcggc gtcactacct aaatcacgac gattagcctg cgcttgggta accttacggg
    30481 tgggataggc ttgatgagcc ggataactaa gtagataaaa ctcagccggc ttttgggttg
    30541 aatcactttg aaaaacaatg tcatgtcgtc ccttgccaat gtataaggct tccttatggg
    30601 ctaaatgaaa tgactcttct ccgacagata caatacccgc ctcaccaata tttataatcc
    30661 cgatttcgcg gcgctcggta aaatattccg ccgctaattc ctttgaaccg ctcaacgtca
    30721 gtggtttgtt tgccggtaca gccgcaccaa cgatcgcgcg gtcggcatcg ctgtaaacta
    30781 aacgcagttc accggtctga aataaatttt cgaccaacat attttgccgt aattcagacg
    30841 ttgtcatacg ttgaaatcga attggatcgg ctaaatagcg aatttccata ttatacctca
    30901 ttaattattt tttacttact ggaacgacct gtcaaaacag ccgatcgaaa agcttcggca
    30961 tgtttggcta aagcctgcca atcatcggcg gctatcatct ttttattcaa cagcgccgtt
    31021 ccgactccca gacagcaagc tccagaccgg atgaattctg cggcgttttc gagggtgact
    31081 ccgccggttg gcaatagttt gacttgtggt aaaggaccaa gaatgtcttt gatataactt
    31141 ggcccaagcg ctgtagtcgg aaaaaccttg accagatcgg caccctgttc ccaggcggtc
    31201 agaatttcgg ttggtgtgaa tgctccggga atggcaggtt tgtcgtagcg atgacacatc
    31261 cgaacaacct ccggattaaa aatcggacca acgataaatt gggcgccgtt taaaatcgcc
    31321 agccgggcgg tctcggcatc cagaacactt cccgcaccaa tgagaaaatc tttgccggtc
    31381 tgcgccgcaa aatttttgat tatctccagt gctcccggcg tagtcatagt gatttccagc
    31441 gcatttatcc cgccctgtct caaagcggaa attatgcctg gtaatttttc accgctatct
    31501 aatcgaatta cggcaaccag tcctgattca atcaattttg cagtaattgt ctgtcggtcc
    31561 attctattcc cttattatga tttaaaggtt tgaattaatt tttattatag aatcattccc
    31621 aattttaaac tgctgaatat taaagttgtc atacaaattt cgcaagtgtt tttattatta
    31681 actggctaaa aagaacttat ttcctttaga aaaataattt atttagccaa atcggtaatg
    31741 atctggagag tatgttcctc agcgatttgc aggtgggttg tcatagcctc aaaagcaccc
    31801 tgagagtctt taacctggat tttttctaaa atcaggtcat ggtaagtgat ggctgaactc
    31861 tttgcgctgt caatttgttg atacaccaga ctgcgaattt tcggcattag ccggaacatt
    31921 ggttcgacga taatcggcac taattggttt ttggaagcga tggcaatgtt gagatgaaaa
    31981 tctttatcga tctgaccctg tttgagataa tcggtggaat cacatttttt caaatcatta
    32041 caatttttcc gcaatttcgt caagtcgcga gcagtacgtc ttgcggcggc aaggctagcg
    32101 atttgcggtt cgaggatttt gcgaatttcc atcacactca tcagataatc tttgtcaagg
    32161 ttcagctcta ggaagagact catcggtttg attacatttt cggcttgata atcagtaacg
    32221 tatatgccgc tccccttgcg aatgtgcaaa agtccgcggg cactcaattt ttggagcgct
    32281 tcacgtaagg aagtgcggct cacgccaaac attttgcata gttctgtttc cgacggtaat
    32341 ttttcaccgg gcagaatttt tttttgacga attagttctt caatatgttc gacaatctct
    32401 tgactaagac tgagcggatt accgatccgc ggaaacagat tataaattgg ttgactatta
    32461 ttttttggcg ccattctgct ttgattttag gcagaatata gattatttgc aaggaaatgg
    32521 atttatttgg aggacatccc aaattccatc tcatttagta taaataggaa gctactacgt
    32581 ggctattcct ctgtagattt agtggtgttt ttcatcattg tttcggcatg tcagtagtcc
    32641 tgtgttaatc cgtgatagat tgtaataaca actcaattta cattttctct tttctcagcc
    32701 tttgccttat tccgacttgc tcaaagggca ttttcaatga cgcccataat tccagaagtg
    32761 gatttcagcg gttgcatcaa gcagtcagcg gataaagtca ccccaatatt ttccgatggt
    32821 ttaagtattt cgaaaataac cttctgatca gtgaggggcc agtcccggaa accgggactg
    32881 acgcggcgca aagtctggct gggagataac caggaggacg atttttcaag atactgttgt
    32941 aaatcactgg tagcggtttc gaccagggcg gcggctagcc cgtttaataa aaatgcctga
    33001 taggcatcgc cttccgtgta atttaaatcg gcaatccgct tatcaatcgc cgaaccaata
    33061 gttacaataa atataaagat cggtttaggt tttactgggt cggatttatt cacaaaacgg
    33121 tgaaggcttt tgccgtgtaa ttcatacgta ccaccagcaa gcgccaagcc ggtttcggtt
    33181 gggaggcagt caaccaatcg aacgatagcg gccggctgaa tcaatgccct attttctttc
    33241 aaaagattag caagttccac gcgcatacga aaggcgacac tggtattttt taaaatgtta
    33301 tatttcagtg cggcggtcaa ttcccgcaag aaatgctgaa aaagtactaa ttgatgatat
    33361 tgttcattcg gcgagattaa ttgtttatta ttttgacgca tttggaagat ttgggctaag
    33421 gaaggagttt caccgttatt tttttcaata gaaaacctga ttgctcctgg gtacccggta
    33481 atctcttgca taagtgtaat taagtcgtcc tgtttaacca ggaaagagca aggtttgaaa
    33541 atcgttattt tagtgaaatc tatcggcatt ttttataagg ctaagataat ccaaaatttc
    33601 ggataatccg cgtaagtgtg ggtgtggatt ataatcgagc gcaatatcat ttctgactac
    33661 tatgcctttg gaatttgctt cgtcagggac agaattgttt aattttctgc attatttctt
    33721 ccagtttctc ctgatcgcgg aaaatgtcaa actgatcggt ttcgacaatc aaaatattac
    33781 tacgcttctg ctcctctttt atccaacgtt cataagagag attcaaggta tagatatatt
    33841 cgggggaaat gtcacgctcg aagccgcgcg aacgcttttt aatgcgggta agaagggtgt
    33901 caacgctggc gcgcaaatag acgatcaagt caggtttttt cagataggaa gtcattattt
    33961 cgaagagagc ccgataattg tcccaatcgc gctgactcat attgcccatt tcgtaaagat
    34021 tacgggcgaa gatttccaca tcctcgtaga ttgtgcgatc ctgaaccgtg gtgatgttgc
    34081 tttcggtcat ttgtttatga ctgcggaaac gatgggagag aaaataaatc tggagattaa
    34141 agctccagcg cttcatatct tgataaaagt cttctaaata aggattgtca ataacggatt
    34201 cgtaaaaagc gcgccaacca aaacgccttg cgatcaggtc agtcatagtg gttttgccaa
    34261 cgccaatgtt accggcaatt ccgatgaaat agggatgttc actcaccgag caattctcct
    34321 tctatataat cttcggaaac gtggattaat ttaacttttt cgattttgtt cagcaggttt
    34381 tctcgtgtgt ggatgcgaac ttttaggtaa ttgtccgtca agccctgcca gagtccgggt
    34441 gctatttttt cttcaaatag aacggagaca gtttttgcga gcattgattg cctaaaagca
    34501 gcccgttttc ggttacttaa ttggcgcaga atttccgagc gccgtttctt ttcggaatcg
    34561 ggaatacggt cggtaaattt gccagccggg gttccgggac ggggcgaata gcggaaaacg
    34621 tgcaggtagt taatgtcgag attttcgaga aattggcgca tctggtcaaa ttccaaatca
    34681 gtttcgccgg gaaaaccgac cattacatcg gcgccgatgc agatctctgg tatttgttgt
    34741 ttaatccggt tgataagtgt ggcataatcg gcgctgagat aacgacgatt cattttggct
    34801 aaaattggat cggaaccgtg ttgcaaaggg atatgcagat ggcgacaaag gcgcggctct
    34861 tcggaaatca gtttaattaa ttccgaagtg attaagtccg gctcaatgga actcagccgg
    34921 atacgcggca aaccggtcgc ggcgagaagg cgccggatta aaaaaaatag atcgccgctc
    34981 ggtgattcat agcgaccaag attgataccg gtcaggacga tttcctgaac gccttgattc
    35041 tcaagaagtc gagctgtggc aatgcaatcc tccggatcgc gactgcgagg attgccacgc
    35101 aaatggggaa ttatgcaata agcgcaataa taatcacacc cttcctggac ttttaaatta
    35161 gctcgaatgc gtttatcatt cggtaagtgc gattcgatgc ggaaagtttt tggagattcc
    35221 ggtttagtaa ctcgtacaat tttttcaccg cgctcgatcg tttccagaaa atcgaatatt
    35281 tcgtacttct cattattacc cagcacgaga aagacaccgg aaatattaag caattcggcg
    35341 gcggcaatct gggagtagca accgataacg gcaattttgc cattgggcga aatgcgtcgc
    35401 gcggcagtga tggcgccgcg ggtcttggct tcggcggctt gcgtcacggc gcaagagtta
    35461 ataatagtca aatcggccgg tttattggaa tttactatgt ggagattacg attggcaagt
    35521 tcggctttaa tggcatctgt ttccgcctga ttcaggcgac agccaagcgt tcgaaatgcg
    35581 accgttgcaa tcaagtctgc ctgatttcaa ttatactttg atcgccgata atgaaacgat
    35641 tggtaaaagg tttagcttgt gattcgatga tctggacgtt tttaccgagt aggctggctt
    35701 cgatccgaat tttttgattg cggatcacgc agttacagga aacgatactg aattcaatct
    35761 cagcattgct gatttcgcaa tttttatcta ttgcggtata tggaccgata taggcattga
    35821 cgatttttgc accttcgccg ataatgaccg gaccgcggat gttgctattg ataatttcgg
    35881 cgcctttact gatgaccacg cgaccgctga gctgactatt ttgatcaact ttgccttgga
    35941 tagaagttgt gatattatcc agaactaaac gattggcttc taacagatcg ctgggtttgc
    36001 cggtatcttt ccaccagccg gtaatttccg aaaaggtgac tttgtaacct ttatttaata
    36061 aatattgatg cgcatcagag atttccagtt caccgcgcgc actcggttgg atattattga
    36121 ccgcctcgaa aatggtgtgg tcataaatat aaattccagt cacggcgaaa tcgctgagtg
    36181 gcttttgggg cttttcgaca atactgacga tgcggttacc tttgatttcc ggtacgccga
    36241 aacgttctgg gtcagggact ttagccagta cgagatgaca attggtctga ttacgctcga
    36301 attcttcgat aaagcgtcgg ataccgccga tgattatatt atcgccgaga taaaaaacaa
    36361 acggttcgtt gccaatgaac ggctcggcaa tccggacgca atcggctaaa ccggtcggtc
    36421 gttcctgagg aatgtaaacg attttcagcc caaaggcttg gccgtcgccg taaacttcac
    36481 ggacttcgtt gccttcttta ttggtaatta ttccgacttc ctgaattccg gcatcgcgga
    36541 catattcgag ggcatagttc agaattggtt tattggcaat cgggatcagg tgtttattct
    36601 gagtatgggt gattggtcta aggcgtgtgc cgtgtccgcc ggctgttatc aatgctttca
    36661 tcgttcattc atcctgatta ttattaaact aaaatctgac caaaaattag ctaatttgga
    36721 tttaactaac aaaagtttta accgttggtt tcagctcgta ggtctaagtt ttccactccg
    36781 ataatggtta cttcataaaa atcgccaatt gagagaattc ttggtgattt tactcgaaca
    36841 ttgcagtcaa tttccggagc atcccagaca gagcgcgcga tatactcatc accatcttgc
    36901 tcctcaatca gcacggtaat tttctgaccg attttacggc gtgcaaagtc ttcaccaatt
    36961 tcccattgaa tttgcatcaa tagatcgcgg cggcgctctt tttccggttc cggaatatcg
    37021 tcgtcaaact tttcagcgac tgtgtcctct tcggcactgt aagtaaaaac cccgagccgc
    37081 tcgaaacgga tttcttcgat aaaattcagc aattcttcaa attcggcttc cgtttctgaa
    37141 ggaaaaccga ccataaccga ggtgcgcaga gccacatcag ggcggtaccg gcggatattc
    37201 cgcaaagtcg ccttaagcct tttaggattg gcaccgcggt tcatcaactt caggaggcgg
    37261 ggactggcat gttggattgg aatatcaata tatgggcaaa actctggatg ttgagcaaga
    37321 atatggttca agtcatcgcg ccagaaatcc ggattggcat acattagtct cagccaggga
    37381 aaaagccgtg ccgcaagtaa ctcctccagc agagttttta aactaacttt tccgggcaaa
    37441 tcactgccgt aacgagtagt gtcctgagca attagaatca attccttaac tccttgtgat
    37501 ttaaggtatt cagcttcgcg caggagtgat tcgattgacc gactgcgttg gcgtccgcgg
    37561 atcaatggga tagcgcagaa tgaacaggaa ttgtcacagc cttcggcgat tttcaggtag
    37621 gcgaaatgac gcggtgtcat tagctggcga atagtttcca gatcgggaca aatattttgg
    37681 ctaccggtca gaaatcgaat cacctcactt tgcgattcga cgccgaataa gccgtcaatc
    37741 tcgggaattt cagccttgat ttgcgcatag taacgctggg agaggcaacc catcacgacc
    37801 acttttttga cgcctgtttt tttgagtcgc agggtttcca gaatcgtttc aatagattct
    37861 tctttagccg gttgaatgaa accgcaagta ttgataataa cggcatccgc ttccgccgga
    37921 tcggctttga attccattcc agcctgcatt agaccgcctt ttagaatttc ggaatctacg
    37981 gtattcttcg ggcaaccgag agagataatg gcaaaagacc gaacagctgg tattagagac
    38041 attaatgagc taattttctg ctaaagaaaa gtatccaaga gaattttatg agtttttata
    38101 ggcatttaag gatgatactg tgatcaattt taatctattc attcaatgtt taatagatcg
    38161 cggatatact gattcggatc aaactctact aagtcggtta atttttcacc aacgcccagg
    38221 aagcgaatcg gaatattcaa ttcgcgatgg atggcaagag caattccgcc tttggcagtg
    38281 ccatccaatt tggtcaggat tagcccagtt atcccaatgg ctttctggaa ttctcgcgct
    38341 tgcaatattc cgttctgccc gacggtggca tccagtacca gccagacttc gtgcggggca
    38401 tcgggaatta ccttaccaat gacgcgcttg accttggcta attcttccat aaggttggac
    38461 tttgtgtgta agcgaccggc agtatcaatc aggagaatat cggcattgcg tgcttgagcg
    38521 gcgcgagcgg catcgaaagc aatggctgaa gggtctttgc cctgtgggtt acctatgaac
    38581 tctacaccgc ttctttcagc ccagattcgc aactgttcat aagccgccgc tcggaaagtg
    38641 tcggcggcac aaatcaatac gcgctgacct tcgctcgcat attttgcggc caatttgccg
    38701 attgtggtag ttttgccggt gccattgact ccaacgatta aaattacagt cggtttgtga
    38761 aaattcgttt tcgtcgctac ggcaggctga attaaggcac tcaactcatt ctgcaggatt
    38821 tcattcaagc gggccgcttc tttgcgatca actttgcgca ggcgttcgat taatgtttcg
    38881 gttaattcta ctccaaggtc ggtttggatt agagtttctt cgatttcctc cagcgttttt
    38941 tcatcaagcc ggaaaaaccg ccctaataga ggtaggcgac tggctagaaa acggccagtt
    39001 ttatttaaac caaaattaat catttttaga aaggttggct ttcttctcgc ggctagcgat
    39061 tattatttgc cgagaatatt cataaatata ttgaaccaga gacagtgcca taaaaattat
    39121 ggaaacaatc atcaacggct tagccgatga gccgaaaaaa ttgggataga tgaaagccag
    39181 cacggtgatt acagtaaaaa caactgagac ttttcccagt ttatttgact gtggggaaac
    39241 cttgctatta ttcaacatat aaattccaaa taccgaaata aggaaataac gaacgagcat
    39301 tatggtaaaa tagaaagtcg ggatacgttc cttgactgtt aagaacagca taacgctcat
    39361 aatcacgacg ctatcggcaa atggatcgag aatcttgcca agcacagtga tttcattact
    39421 ggagcgcgct aaccagccgt ccaacatatc ggacaaaatc gcgatcagga tgaaaataag
    39481 agcggtcttg ctgtcattat tgcgcaggga atagacaatc ggtgccgcta aaaatgctcg
    39541 aactaaagaa atgatattgg agatagttac ggttttacgg gcagggatgg cgtcgtactt
    39601 ggaggtaatg ttacgtttga cctgaagagt gtgtttaatt ggatttttac tcattgctgt
    39661 tcctttgata ggacttttcc aataaattca atcctacctt gtgaattata gaaaaatttt
    39721 gatccagtat tttccattcg ccttcaaata tcacagaacc tttatcaaaa attaatatat
    39781 tgtggcataa tttacgaagg agacggtagt cgtgcgaaat aatcataaat tctttttgcg
    39841 aatcgtattc tgggagaatg ttttcaaatt tcgtccggct ggacgggcta agagcaatcg
    39901 tcggttcgtc gaaaatcgtt aattggggcg caagcgtaat acccaaagca atagtagcca
    39961 atcgcagttc accgctactc aattcataac cgtagcgttc gcgaatgagg ttataatcca
    40021 atcctaattt ttgaaggtta tcggcgaatt tagccgtcaa ttcagccggg tcgttccagc
    40081 tcttgagaat tttctgcaag gcattgccga tcgttattcc gaggaaaatt tgctctggaa
    40141 attgcggcag ataaaggatg cgcggtttag ttttactgct ggataaattt ggctcgggtg
    40201 cttcgatttg aaaaaatccc ttcgtgggag ttaataaacc gcccaggatt ttgccgaaag
    40261 tgcttttacc ggaaccggtc agaccgtaaa caccaagagg ataattcaga gggcaagtca
    40321 attcgggaat agataaacgg aagttcggat tttgtggata ttggaattcg aggttctggc
    40381 agattaggta attcatttaa ttgtaaaaat aggattattt attcgaactc gtccggagta
    40441 aatagccagg taatagctca attatgtaat cggcgaaagc gatttcataa tcttcttgag
    40501 aaatccagat gacgctgagt ccagcgacga ttaaccggcg aatattttca taaagttcaa
    40561 aacgagtcgg caagtcgaga aaagcggtgg tttcatcaaa aatcagaact tgcggtttgc
    40621 ttactaacca atcagcgatg gccagtttct gcttttcacc accggatagt tcatgcggtg
    40681 aattctcacg gcgcggccat agattgacac tctccaaagc aagttgcacg gccttccgga
    40741 cgatagcggg actggcaccg cgattttcca aattaaaggc aacttctcgt tcaatattga
    40801 agtggacaaa ctggtcatcc gggttttgga aaatatagcc gacttctaat gaattattga
    40861 gaccattatc attttcaaat cggattgagc ctttatccgg cttgagtaaa ccggcgatta
    40921 atttagccag ggtagtttta ccagtgccgc agtcaccagt caaaactgcc cagcaattta
    40981 ggggaattgt caggtcgaga tttttgaaaa caggttggtt tccgtggtag gaaaaatcta
    41041 ttccgtcaag tcgaagtttc attccgactc caggatttct tggacggttc gggatgaagc
    41101 gcccatattt tttagaatca cctggcgggc agattgggcg gctttctggt aatcgtccgg
    41161 tttagttatt agatgtttga tggttgaata aatacccggt tcatcaaggc agaccagtcc
    41221 gccaccggac tgcaccaata attcggcttc ctgggaatta tgatacgatg gtccaaagag
    41281 caccggcaaa ccggctaccg ctggctccat aacgttatga attgagcccc ggaaactacc
    41341 gccaacgaaa gccagattcg cctggtgata aagttcagcc aggataccga tacgatcaat
    41401 aagcacaacc cgtacattgg caggcatgcc cttcaattgt gaataacgga ctgtctcaat
    41461 attatgcgtc tgaaattcat cctccagcat ttccaaagca tagtcattgg gttcatgcgg
    41521 cgttaagata attttaaaat caggaaaatt cttcagacat tggtagagag ctggtagaag
    41581 cggacgggca tcctgaggcc aaatgctacc accaatgaaa acaaatccat cgttaaaaac
    41641 tcgcgtaacc attttattag cgagtaattt ggagcgctcg ataacttgat catagcgagt
    41701 gtcaccgagg ttcaatacgg taatcttgac tgagccgatt agcttcagca ccgattgttt
    41761 atgttcttct gaaatggcat aaatataatc cagcgaacgg taaaggtcag cgaaaaaatg
    41821 ccgaatgatc ggctttagtt tggaactatc tggacgaatt cgtgctgaaa tcagataagt
    41881 ttttatttta tatcggttag catggaaaat gagattcggc cacaattcgt aagtcacaaa
    41941 aataatttta gtcggtcgta atttctgtaa aaagcggcgg gtttgtaaga aagtatcaat
    42001 tggaaggtaa gtaaataaat caacttctgg cattcgttcg gcatttttga atccactggg
    42061 cgaggtgaaa ctggctacca gcaaaatatc cggacgcagg gttttcaagc cgcggatcac
    42121 cggtttggct tgctcaaatt cacctaagga tgccgagtgg actaaataga ctgtacgttt
    42181 aggcaattct tcccggaatt tctgtaagtt tagccagaga gtctgacgtc ctttcagtcc
    42241 ttctttaatt ttggaattaa aatgtgatag aatcatcaat ccagaatagg caatgggcat
    42301 aacaatagca ttataaatta ccagccagaa tatagtcaag actcggctcc tattattttt
    42361 accagggcat cgtaaacctg cgcatcggtg atttgattaa aacataattg ccgttggcgg
    42421 cgatagcaac gccgatcacc tttttgagaa caaggacgac aaaatagttg agcttccagg
    42481 acaatacttt ttggtgcgta acagccggcg ccggtttccc gcgcggtcgg ccccagaatt
    42541 aatatcactg gttttcccag ggcctccgcc gcataggtca agccggtatc attaccgatt
    42601 accgcccggc tgagaccaat cagcgccaga gattcgctta aggtggtttc gcctgctaaa
    42661 cgaacactct taccaggaac taaataatca agctgttgca ggtgaatatc agcttttcca
    42721 ccaagccaga gtaccggcat ctgtagatcg gtgttgattc ggcgcgccac attgatatat
    42781 ttttcgagag gaaatagttt atttggccaa tttgcaatgg gcaaaatgat aatgaacggc
    42841 tcggaaattt catatttaac aagaatatcc tgcgctttgg taactgcttc cggctcgaga
    42901 aaaatttccg gcttagcggt cggcggatag ttaatcccaa gaggctgtaa aactttatag
    42961 tattctttca aaagacggaa atcgtgcgag aaaaaattca gccagaagta gaaaagtaga
    43021 aaacgtctca ggtaaggttt atgataccgc agaagagaag taccccgcaa ataatgagaa
    43081 agaatatgac tgcggatatt attgtgcata tcaatcagta aatcgtaatc ttgggctttc
    43141 aattggcggc ataaatctcg ccagccagct aattttgtcc ccgaatcgta agtgatgata
    43201 tttgatagaa aaggattatt ggcaactatt tcagcgaatt ccatcttggt cagaaaatca
    43261 atgcgggcta aaggatattt ctcccttaaa ctacggaaaa ttggagtgag aacaacgatg
    43321 tcacccatcg aactcaagcg catgatcaga atagaattgg gattagtagt cgttgtcaat
    43381 tatttctcat aagcgtgtag ttagccagat taattagggc ggatttatat tgagagtcgg
    43441 ggaaaatagc aagtgccgca acggcttgct ggatcaactc ccggcattta ttttcggcat
    43501 aagtaatgcc attggtattg ataatgagct ccgttattgc ggttatctgt tcgggagaag
    43561 ggcgattttt caactgctgg cgaattcggt ggcgttcttc gggcgctaag gttttcaaag
    43621 cgtggattaa cggcagagta actaagtttt gcttaatatc ccgcgctacc ggtttgccgg
    43681 tatgaataga tgtgccggtg caatcgaaaa gatcgtcttc gatctggaat gccaatccga
    43741 gacagcgacc ataatcgaag agagccttgg tctgttcttc attggcggca accgtgagag
    43801 tgcccacctg acaagcggtg gcaaaaagtg aggcagtctt agcccagatc acttgatagt
    43861 atatttcctc atccatattg ttgttcaggc ttttttccaa ctgtaataat tcaccggaac
    43921 tgagcaattc ggaagtttga gaaagtaatt cgagaactgt aaaatccttt aaccttaaca
    43981 tattgcttaa agctcgacta aaaaggaagt ccccggctaa tacggcgatt ttattcgacc
    44041 agatggcttt gacagaaggc cgaccgcgac ggacttccga atcgtctata atatcgtcat
    44101 gaaccagagt agcagtatgc agaatctcga cgacactggc ggcgatgatc gaatgttcgt
    44161 tcgatttacc acagagattg gcgctcagca gaaccaatgc cggccgaata tatttgccgc
    44221 ctcctgataa tatataactg ccgatctggt tgattaattc cacttcggaa tataaaactt
    44281 gtctgaattc tttttggaat agatcgagct cagtggaaat tggtgcaaat atgttattta
    44341 aatctttagg cggcattatg aactatccag ccaattctat ttgatcggtc tcattttgaa
    44401 ttttcgggcg actggcgatg cgcgaaatcc agatgctgat ttcgtataga aataataaag
    44461 ggagggtcat aaaaatcata ctaactggat cgggtggagt aataatagcg gcaataacca
    44521 ttaaaataat gatggcataa cgccacgatt tgcgcagaaa ttgcggcgtg attagcccga
    44581 ttttggtaag gatgaaagac aaaactggca tctgaaaaat caatccagtt gcgagtatca
    44641 actgcatgac gaatttcgtg taatattcga tcgaaatgtt gcgttcgaca ccagctacac
    44701 caatcccaat cagaaatttg agagctatcg gaattagaac aaaaaaggca aaaattgcgc
    44761 ccaatagaaa gaatacggtt actccgatta ttaatcccgg gccccaacgt ttttcatttt
    44821 gcagaagacc tggtgagata aacgcccaga attgataggc aactatcggt agagcaatta
    44881 ccaaaccgag tagaaaggca accttgaatt tcagcatcaa taagccctga attttgagaa
    44941 cctgaatagt catctcggag ccaacttgcc gcgacggacg catcaaaatg tcaattgctt
    45001 gatcagaaaa taggaaagcc cctacggtgc ctaatactag tgcaatcaaa attttgatga
    45061 tccgccaacg cagttcttcg aggtgatcca gaaaccccat ttctttttcg gcattggtag
    45121 aattgattcg cgccattgtt aatggtaaac ttaataaata ctcataattt ttttcaaatg
    45181 cggtgtaaat ataaatatcc tacacgtgaa agtcatagat tattactaat cctcatataa
    45241 tagagaatgt ctattttatc ggtaaaatta gcagggttcg agagatttgt atttatttat
    45301 tatcaaatta tgggtgaagt atctaattgt tatttttata aataaaatat ttttctttga
    45361 attaacctaa aattgtgtag tcagcacgta cttaaggagg tcgtgcttca gctgaattgt
    45421 ttaacgaatc cgtcgtagtt gtttataatc agacgacgac catatttctg gatataacct
    45481 ttacgttgta gtagagtcaa cattcgtgaa acagtttcgc gggaagtacc agccatatta
    45541 gcaatatcct gctgataggg caggttttct attatgacct ttccctgttt gataacgccc
    45601 agatcttccg ctaagcgcag gaggcacatt gcaattcggt tctcagcatc acttagcgaa
    45661 agtccttcaa tttgctgatc actacggcgc agacgcgtag ccatttcctg gagtaaagcg
    45721 atggctatct gtggatgttt ttccagcaaa tccaaaaaat cgccccgttt tagaattaaa
    45781 actgcggtgt cttccagcgc gacaacatta gctgaccgat tttcgccatc cagaattgac
    45841 atttcaccga aaaaatcacc ttcaccgagt atcgaaagaa ttacctcacg gccatcctca
    45901 cccattctag taattttgac acttccttta ctgagaataa agagcgtatc accgaattcc
    45961 tcttccatca ggataatgtt gcctttttta tatgcccgct tttgcataag gttatagatt
    46021 tgctggataa tatcatcctt aagatcggca aataatggaa tattttttaa tatatctatt
    46081 tccatatcac tactctcata tataaagcgc taagaaaata ggttcactcc ggcaaaaaac
    46141 caagaaaaac ttttcaacaa ttttcgatga aataagtaat cacgtatttt ctttggctga
    46201 ttttttctcg gatttaggtt taacatactt ttccagcact tcaggaacga tttccataag
    46261 cttgtccacg ccggatattt tctctttcag ccggtgaatt ttcacctcac ctttctggat
    46321 tgtaatgact gcaagcggtt cgatcaaagc gccgcctcct aagccggtgc cctcagtctt
    46381 ctttttttgt tcttctggat taccgctact gcccaccata aaaccgagag aaattttcgt
    46441 aatagggatt accatcgtat ctccagtttc gattggtttc ccaattactg tttcagaagc
    46501 tacaatttca cgtaaacgac ttaacagggt gtctactaaa ccttccagca taatttacct
    46561 ccgtttttta tttttcattt ttctcaaaaa attcactata aaaataagta taggaattgt
    46621 ttttatcaat ctaatcgatg cccggaattc gactttaccg gtgaattttt tggcgacaaa
    46681 atctgggacg atatgcactg attggaaatc tttcactgtt tctcgaatcg tgtaataagc
    46741 gcccattaaa agaccggtat aggccggatt tcccaaaccg acggtaagat ttgcgcttaa
    46801 tgtctcaata tggaccaatt taaaaaatcg tagtgtcagt gctttcccaa actcaatcca
    46861 gtcaaatatt gagaatttat accgcctggt tcggggcttt ttgggaatct ctggtttggg
    46921 ttcgggtgat tttttcttgg ctagtgaggg ggcttttttt tgcccaacct tgaaatgcaa
    46981 gccgaggata ccaatacgaa aatttacctc gggcgtttcc atcacaacta taatgctact
    47041 caatttaccg gctaactgaa ctttcagcgt gccagtttta gtttgttcgc tgaaattccc
    47101 aaaaatactc agccaggttt tcgaaaatag agttacaaaa aggaggaaaa tcaaaataaa
    47161 aaataagacg agaagtattt ttagaataat catcattcgt ttcggaggtt ttttatttga
    47221 aaatgagctt caatataaat aaatttgcct actttttggt aatctttgtg aaaggagttt
    47281 aatgaaaaag aatgcgtctg ttttaaaacg aactcgtcag gctcaaaaag ccgccttaag
    47341 aaatcggcac tatgtctcaa tgatgaaaac ctcgattaaa aaggttaaaa atactaaaac
    47401 taaggctgaa gctgaacccc ttttcaaagc cgcagtggca ctgatagaca aggtcgctcg
    47461 gaaaggtatt atccataaga ataatgccgc tcaccgtaaa tcggcactgg ctgagtattt
    47521 agctaatctg tcctgaacgt cagtagtata attattgccc tctttaattt tccaagcggt
    47581 aattcggggc ttctttactg attttaacat tatgtggatg gctttcctga atggatgcct
    47641 gcgtgacacg aataaaacat ccccgtttct gtaattcatt tattgttgca gtgccgcaat
    47701 aacccattgc tgaacgcaga ccgccgatta gttgatgaat tgtatctgct aaagagcctt
    47761 tatatggtac aatgccttca atgccttccg gaactaactt gctggtttct tcaccctcct
    47821 gaaaatatcg gtcgctactg ccatgagtca tcgccgcaat agatcccata ccgcggtaag
    47881 atttataaac acgaccttcg tataataccg tttcacctgg actttcgtcc aaaccggcta
    47941 aaagtgaacc aagcataacg gtactggctc ctgccgctaa ggctttggca atatcgcccg
    48001 agtaacgaat cccaccatcc gcgatgatcg gaatcccgct tttcatcgca acttcggcgc
    48061 aatccaaaat ggctgtcaat tgtggaattc ccaccccggc gatcacccgc gttgtgcaaa
    48121 tggcaccggg accaatgccg actttaaccg catcggctcc ggcgtctatt aaggctttgg
    48181 tagcggccgc agtcgcgaca ttgccggcaa ttacgtctac ctggggaaag cggcgtttca
    48241 actcgcgtac acttttgatg acattggtag aatgtccgtg ggcagtgtca atcaccagca
    48301 catcaacttg ttcactgact aatttttcgg cacgtaagag gtaatcggaa ccgacaccaa
    48361 ctgcggctcc gacccgcaga cgcccaaatt catccttggc ggcattcgga aaacgtttct
    48421 tcttcagaat atctttaacc gttattaatc ctttaagccg tcctttatcg tccacaatta
    48481 agagcttctc aatgcggtga cgctgaagaa tttcttcggc ttcttcgagt gtcgtgcctt
    48541 ccggtgcggt aactaaattc tccttagtca ttctttccga aatgcgcaga tcaaggttgg
    48601 tctcgaaacg aatgtcacgg ttggtcagaa taccttttaa ttgaccgtta tctaccaccg
    48661 gaatccccga aattgaataa cggttcatta gatttagagc atcccgaatt gtactatcgg
    48721 aggatagtgt gattggtttg acaatcatac cgctttcact gcgtttcacc ttgtcaactt
    48781 cggctacttg ttgttcgata ctcatatttt tatgaagaat accgatcccg ccttcgcgag
    48841 caatggcgat agccattttc ccttccgtca cagtatccat agcggcacta agtattggta
    48901 tgtttaattg aatttttttg gtcagcttgg cgtcgagact gacctctttc ggcagtacgt
    48961 ttgattcaga tggtattaga agaacatcat cgaaggttaa gccttcgcgt gcgatttttt
    49021 cattcataga caacctcata tattttggta taaatatatc gataacaatg gcttccggtg
    49081 tatcgtaacc ggggaataat taataggtaa gacttccggt ataattccca ttcgactaag
    49141 cttgtcgaat tcggtatcaa cgccgtaaat agctttttct ggactaaaag ccgatgaaac
    49201 aacctggcag gagcattttt gtttccggcc tttattcttg gcgcttttga gaaattgaat
    49261 tgttttgcct accattaagt aaactacaaa aaaataagaa attttcatag tgttagaaaa
    49321 ttaattttac gaaaatattt aaacaatatt ttacccgaag ccgatcactt taaaactccc
    49381 taactgataa cttgactata aaattgattt aatgtatatt gcattgtaat aaaagaggta
    49441 agcgatgcaa tccatgaaaa aaatatttct gatctggatg tttagcacaa tcgcatttgc
    49501 ccagcaagcc gtcgaggttt cggatataat aatttccgat ctggacaata acttaattga
    49561 aattagggtc gaggtaaaaa atacttcgat gaaatccatt agcgaattag ctggatttat
    49621 agatatttac gataaatacg gcaacattat tgaaaaacag gaaattgtga ttgtgctgaa
    49681 tagcgatatc ccgcttaaac cacaaaatac tgcttcgggc agtataatta ttacccagcg
    49741 tccgaatatg tccgggcagg tgaattttcg catcaccaat ttacgctatt tcggtgaacc
    49801 gaatgtttat ctaatttgtc cggtttgtgg agaactcatt cccaaagatt gatcccctga
    49861 acaccccggc aaacggctct ccgtaactca ataatcattt ttaccggcat ttgccaaaat
    49921 acgttaggac attcccaatt tattttttac tccattatag tttgataaaa aatcgttgtg
    49981 aattctcata ccatttttaa ataggaggca gtaaatttat ttatgaattt ggatgcacta
    50041 cgggaaaagg ctaaggaact ccgccgtcaa atagagactg ctaattacaa ttactacgtt
    50101 cttgataatc ccacaattag cgatgctgaa tatgatcgcc taatgcgtga actagaacag
    50161 ttagaggcgg cttatccgga attgagaacg ccggattctc ccactcaacg cgtcggcgcc
    50221 caacctttag acgagttcaa aaccgttacc catcgcgttc ctatgctcag tctggacaat
    50281 gcaatggacg agggcgaaat ccgcgatttt gcaattcggg tgcaaaaaag tttgccgggt
    50341 gagaccgtcc agttcgtatg cgaaccaaaa attgacggtt tggcagtcga actcgtttat
    50401 gagaatggtg ttttcatttc cggttctact cgtggtgacg gtataaccgg cgaagatatt
    50461 acccaaaatc taaaaacgat ccgcagtatt ccgctcagat taatgccgaa tttgccgatt
    50521 ccgactctac tggaagtgcg cggcgaagtt tatatggata aagctgattt tcagcgtctg
    50581 aacgaacaac aattgaagga aggaaaaccg ctatttgcta atccccgcaa ttcggcggct
    50641 ggctcggtaa gacaactcga tccgcagatc accgctaaac gcccattgaa aatattctgt
    50701 tacggactgg gatctttaga aggctattct tttcaaaccc atcatgaatt tctggaagct
    50761 ttactcaaat ggggctttcg ggtcaatccg ttaattgaaa tagctaaaga agtcgaggcg
    50821 cttatagcgt attatcataa aattgaatcc atccgcgact cgatgcctta tgacattgat
    50881 ggtgtcgtat ataaagttga ttctctagct cagcaggcta aactcggaat aaaatcacgc
    50941 agtcctcgct gggctattgc cggaaaattt aaagctcagc aggaagtaac tcagataatt
    51001 gacatcgaag ccagcgtcgg acggacgggt gccattacgc cggtagccca tttaaagccg
    51061 gtgcaaattg gcggcgtaac cgtatcccat gcaactcttc ataatcagga tgaaattgac
    51121 cgcaaggata ttcgcattgg tgattgggtc gtagttcagc gggcaggcga tgtcattccg
    51181 caagtggtta aagtcatcac tgagcgacgg accggcaacg aaaagcgtta ttcgattcca
    51241 gccaattgcc cggtctgcgg aacgccggtt gtacgcgagg aggatgaagc taaacaccgt
    51301 tgtccaaata ttaattgtcc ggctcagatc aaagccagca tcgaacattt tgctagcaaa
    51361 cgggctatga atattgaagg ccttggcgag aaattgattg aacaattggt tgatagcggt
    51421 ttaataaaaa atgttgccga catttattct ctgcgaaaag aacaattatt gaccctggaa
    51481 cggatgggcg aaaaatctgc ccaaaattta ctggatgcca tcaccgccag ccaaaatgtt
    51541 agtctggcgc gcttcatcta tgcactcggc attcggaacg ttggcgaaca tatggccaaa
    51601 gtgctggcaa aagcatttgg tagcctcgaa cgcctcagtc aggcttctga agaagaatta
    51661 atggcaatcg aaggcgttgg accaatcgtc gcccggagcg tccgcaattt tttcaatgcg
    51721 gaatccaacc gtcaaacaat tgagaaatta ttaagcagtg gtattcggat cagtagcgaa
    51781 caggattaca aacaaaaaga agaaattgcc ggaaagactt tcgttttcac cggcacgctg
    51841 accaatttta cccgcgaaga agcccaagca ctcgtagaaa aatacggtgg caaatctgcc
    51901 tcctccgtca gtaaaaaaac cgactacgta gtcgtcggcg aaaatgccgg ctcgaaagcc
    51961 gaaaccgccc ggcaactggg cgtcaaaatt ctcaacgaag cggaattttt agaattaatt
    52021 agctataatt agaatgagat ttagtattta tctgcaggaa tgtagaatcc acttttctgg
    52081 aaagaaatca ggatgtctga ataaatagaa aaatgctatt tcgagtgact gtcgctttag
    52141 tagtaaagcc aaagtaaacg gctagtcggg ttgtatccat tggaaaactt ctaaaggtta
    52201 attgtttatt ttctatgccg tttttccagc acattcataa tatcgtcgag cgcgacatct
    52261 ttcgctacta atagcacaag caaatgatat agtaagtcgg caacttcctc tggcaggcgc
    52321 atttcattac cggtcacggc atcaatgacg acttcggtgg cttcttcgcc aactttctga
    52381 gcgatgcgtt cgtctccggc atcgaatagc ttattagtgt aagagcctgg tttcggatta
    52441 ttgcggcgat cgcgaataat ctcttccagt tcgaaaatga acggcttctc agctatgttt
    52501 ttttcattga aacaggtatc ggcgccggta tggcaagttg gtccggttgg tttaactttg
    52561 atcaaaagcg tgtcgccgtc gcaatcgctt agaatctcct tgacttctaa aaaatttccg
    52621 gacgtttcgc ctttcgtcca gagtgcctgc cgactccgac taaaaaaagt aaccctgccg
    52681 gtcgcctttg ttttttcata agattctcga ttcatataac ccagcattaa aacttttcca
    52741 gtcttttcat cctgaataat tgccggtaac aggccattat ttttagtaaa atcaatgttc
    52801 ataatcgcac ctcgatacca ttggatttaa ggaatttttt taattccgga atggggattt
    52861 cgccgaagtg aaagacacta gccgctagcg ccgcatcggc ttttcctatt ttaaacactt
    52921 cccaaaaatg ttccattgtg cccgccccgc cagacgcgat taccggaatc ggtaaatttt
    52981 ccgcaagttc acgcgtaatt tccagcgcaa agcccctttt ggtgccatcg tgatccatcg
    53041 aagttagtag aatttcacca gcgccgagct cgacgacttg ccgtgcccag gcttgcgtgg
    53101 tttggttagt tggggtcctt ccgccattaa tatatacgaa gtcgccgttt tcggtatgtt
    53161 tggtatcaat cgcgattacg atacattgac tgccgaagcg cccggctaat ttttcgatca
    53221 tttccgggtg attgaccgcc gcggtattga ttgaaatttt gtcggcgccg gcgtccagca
    53281 ggcgagcgac gtcatcggcg ttgcgtactc cgccgccaac ggtaaacgga atgttcaaat
    53341 gtttcgcaat ttcgcgcacc aattcgataa atgttccgcg tttttcgatt gaagccgtga
    53401 tatcaagaaa aaccaattcg tcagcgcctt gctcggcata agccgcgccg agttctaccg
    53461 gatctccggc acgccgaata ttaataaaat ttacgccttt gactgtttgt ccatctttga
    53521 cgtcgaggca gggaataatg cgtttagcta acataaaaat cctttcaact cactcaattt
    53581 taggtatcct tcataaagcg ctttgccaat aataacgccg tcaattccat catcttgtaa
    53641 ctgataaata tcgtcaattc ggctaatccc accgctggca atcaagtaaa gatcgggaaa
    53701 tatttgtttg atttctcgat ataattcaaa agccggtcca gtcaacattc catcgcgtgc
    53761 gacttcggtg caaatcgttt gttggatgcc tttttcttga aaattctgta aaaattccat
    53821 tatttccagt ggggcttttt cttgccaacc gtggatggca atttttcgat cgataacgtc
    53881 ggcgccgaga atgatttttt cgccgccgaa ttttttcaac cattcttcca ctaaatcagg
    53941 tgcggagatt gcgatgcttc cggcagtaat ttgccgtgcg ccgctctcga aagcaatccg
    54001 cacatccgtt tcgctccgaa tcccgccact gaaatcaatt tgcaaaggcg tccccgttgc
    54061 aatcttttcc agtacttccc agttgactac tcgcttctgg cgcgcgccgt ccagatctac
    54121 cagatgtaaa cgcctgatac cggcatcggc gaattgtcga gcaatttcca atgggttctc
    54181 gttataaatt ttttgagtat tgaaatcgcc gcggctaagg cgcacgcact tgccatttat
    54241 aatatcaatt gcgggtatga ttgtaatcat agtttaatga aatttcgcag gatttgctca
    54301 ccaactttac cgcttttctc tggatgaaac tgcactgcga agaaattgtc cttctgcata
    54361 gcggctgaat agggaatgat atactcggtc tgcgcaacgg tatgttcact caattcggca
    54421 taataactat gaacaaagta aacataggaa ttttcagcaa catcgcgaaa aagatcacct
    54481 tttaaatcga aaagtgtatt ccaaccaacc tgaggcactt tggcgagcgg tggaaatttt
    54541 cttacagcaa tgtcgaaaat cccaaaacat tgagtagcat tctcttctga atcccggcac
    54601 atcaactgca gaccgagaca aatacccagg gtcggtttct gcaaccgtgg caatatctcg
    54661 aaaataccat tcgccttgat agattccatt gcggaagagg cttcaccaac cccggggaaa
    54721 atgactttat ccgcggcttg aatcacggcc gggtcggaac tgacaacggc tttgacattt
    54781 agacgctgaa gtgccagtct taccgagtga atgtttccgg aattatagcc aattatgacc
    54841 actttcataa tttgcctttg ctactcggaa tctggtcgca ggttggatcg cgacgaatag
    54901 ccattttcac tgcccgaccg acggctttga aaactgcttc gattttatga tgttcatttt
    54961 caccggtggc tttaatatgc agagtgcagg cggccgaatc ggcaaaggac ttgaaaaagt
    55021 gacggaataa ttcagtgggc atttttccga tatattccct tttgaagtca cattcccaga
    55081 cgaattcacg tcgtccggaa aaatccagtg ccacctgcgc cagtgactcg tccatcggca
    55141 gaagaaaacc gtaacgttcg attcctttct tatcaccgag ggcttttagc aaaccttcgc
    55201 cgagaacaag ggcaacgtcc tcgaccgtat gatgttcatc aactgcgaga tcgcctctgg
    55261 cagtcaaatc aagatcgcaa ccggaatgcg tagcgaatag attcagcata tgatcaagaa
    55321 aaccaatacc ggtgtctatt cgatttgcac ctttgccatt cagattaatt accaggcgga
    55381 tgtcagtttc gttagttttg cgggtgacgg tggctgtgcg actaatattg cttaaataat
    55441 tataaatttc cgaccagttt ttagccttaa aatcggctga aatagattcg ttgcttccaa
    55501 ggtaaatcgc ctttgtgccg agatttcgtg ccagttgtac gtcagtctcc cggtcgccaa
    55561 ttacaaatga attttcgaga tcgtaatcgc ccttaaggta atctgttaac atgccgatgc
    55621 caggtttgcg cgatggggca ttgtcatttg gaaggctacg atcgattaaa atctggtcga
    55681 ataccacgcc ttcgccggcc agaatttgta acattttatt gtgagctggc cagaaatctt
    55741 tttctggaaa taaatccgtt ccgagtccat cctgattggt aaccattact agttggtaat
    55801 caaattgggt ggcgattttg ttcatatttg tgatggcgcc cggaaggaat tcgagtttct
    55861 ccagtgaatc cacctgttca tccggtggtt cgataatcaa tgtcccgtcg cggtcaataa
    55921 aaagtacttt tttcaacttg caaaatcctt gagtttagca attaaaatat cattttcgtt
    55981 aggagtaccg atggtaattc gcaggcaatt ttcacaatgc caggcttgcg agcggtcacg
    56041 cacgatgatt ttttgattaa ttaaatattg aaacacgcta cgcgcatccc gaaatttcac
    56101 cagcagaaaa ttagcgtcgg aagggtaaac cttttcaact atcggcaatt ctttgagtga
    56161 atcggcaagc cgttcgcgct cggtgcgaag ttcggtaacc atccgatcct tttccgcaga
    56221 tcgttctaaa gccgacaacg ccagctcctg ggtcaagccg ttgacattat agggatattt
    56281 gaggcgcgac agccaatcaa taatctgagg atcggcgaaa gccatcccga gtcggagatt
    56341 cgccaagccc caggctttcg aaaaagtttg cataattacc agattattga actccttcag
    56401 tcttggcaga aagctttttt ggggtgcgaa gtctatgtag gcttcgtcaa ttacgacgat
    56461 tcccttgaat ttctccagca aattgacaac atccgctgag ttaaggcaat taccagtcgg
    56521 attgttcggc gaacagagaa aaatcagctt gacgcccgca atttgctcaa aaatttgcgg
    56581 tagattaatc tgaaagttcg gagttaaagc aacggaaaca acaccgacat cattactggc
    56641 ggcgctgacg gcatacatac cataggttgg cagtaggatc ataatttttt ccgttcccgg
    56701 ttgacagaaa acgcggatca atagatcaat gacttcgtca ctgccgttgc ccagaaaaac
    56761 ttgttccggg ttaacatttt taagggcggc aatttcctgt tttaggcgtt tttgatgcgg
    56821 atcgggatag cgattgaatt tttgtgggat tacgctaccg agagaatttt cattggcgtc
    56881 gagaaatatg ccgatcgcgc cgctataatc gtcgcgggcg gttgcataag gctggaggtt
    56941 aatgatattg cgccgcgcca gtttttccag atcaaccacg tccagctcct ttttcaatcg
    57001 cttccaggcg aattcggact gcgttgcgat gcgcctgcaa cttttcagtg gtagccatac
    57061 aatcaatagc cggagcgatg gcttttagcc cggcaggcga aatctgctga aaagtgattt
    57121 tcttgacaaa gctgtccacc gaaattccgc tataattgcg agcgtaaccg ttagtaggca
    57181 aggtatgatt agtaccggaa gcataatcgc cggctgattc gggagtaaaa tcacccagaa
    57241 aaaccgaacc ggcgttaatg actttatccg cccactgctc gggattttgg gtttgaagaa
    57301 taagatgttc cggtgcataa caattgatga attctaatgc ttcatccaga ttattgacca
    57361 aaatcaggcg actactttcc agtgattttt cgatgatagt gcggcgcggc aattccgcca
    57421 gctgttgctt gaccgccgtg ataacttgcg gaattaattc ttcgttatcg gttacacaaa
    57481 tgacctgact atcggcgcca tgttcggctt gcgaaagcaa atcagccgcc acgaaatccg
    57541 gattagcgaa ccggtcggct acgaccgcca cttccgaagg cccagctggc atatcaatcg
    57601 ctacgccctg agcgctgacg atttgcttgg cgattgtcac gtattgattg cctggtccga
    57661 agattttggc gacacgcggt acgctctgag ttccgtaagc catcgccgcg attgcctgag
    57721 ctccgccgat cttgaaaatt cgctcgatgc ccaaaatttg ggctgtgtaa agaatcaccg
    57781 gatgaatgcg accgtcttta ttgggcggtg tgcacaaaat aatttcggga caaccagcca
    57841 gccgcgccgg aatacctagc atcagcaccg tcgaaaagag cggcgccgat ccaccgggaa
    57901 tatacaatcc gacactttcg atcggcacac ttttacgcca gcagataacg ccaggagtag
    57961 tctcaacctt ttcaattcct tcaaactgag aggaatgaaa tttggtaata ttagcacgcg
    58021 caatctcgat ggctttccgg aggtcatcat ctacttcatt cgggacagat gccaattcat
    58081 cgcgcaggat ctcgatttct ttcaaacgca cattatcgta tttccaggaa aattgccgga
    58141 gggcgtcatc gccgtcggtt ttaaccgctt ctataatttc aatcactaaa tttcgtaaga
    58201 ttttattatc aattgctggt cggcgaacta actctggcca ggaagttttt tgaggatatt
    58261 ttataacttt cataatggca tcctacatta tcattttttc aattggaata accagaatgc
    58321 cctgcgcgcc agccgctttt aaggcggaaa tacgttccca gaactgatgt tcctcgatca
    58381 ccgaatggag cgaactccaa ccaggctcag ccagcggcat aatcgtaggg ctcttcattc
    58441 ccggtaaaat ggcgcagatt tttgcgatgg cggaatctgg ggcattgaga agaatatatt
    58501 tgcgggtatg cgcggcttgg acagcccgga ttctgagaag taattgttcc agcaaatcgg
    58561 ttttttccgg acttaaattt tcagcggcta ccagtacggc ttcacttttt aaaacagtca
    58621 caacttcttt cagcccgttg gtcagtagag tactaccggt gctgacgata tcgaaaatcg
    58681 ccgaagccag tccgataccg ggcgcgattt cgaccgagcc gctgatttca tgaatatcgg
    58741 ccttaaggtt attttcgttc aggaatcgct tcaaaatgtt cggatatgaa gtggcgatgc
    58801 tcagcccatt caagtccgtc aatttttcgt aactaaagtc tttcggcacc gcaattgaaa
    58861 ggcgacactt ggcaaaaccc aatcgctcga caattttcaa cggacgcgct ttttcatgat
    58921 agacattttc cccaacgatt cctaagtcag caagaccgtc agcaacacat tccggtatat
    58981 cgtcatcgcg taggaaaaga attcgcattg gaaaattaga cgcttcggtc attagccgtg
    59041 ccgtactacc attgctgacg aagagtccac attcgctcag caatttggtg gtgtgctcgt
    59101 ttaaccgtcc tgttttttga atagctaaag taatcataat tgctcctgaa aataaaaaac
    59161 ccggtacgct tacagaagga ccgggttgat taacaatacc tgtaaataat ctatctcatc
    59221 ccggttccta ttctggaatg atgatggaga cgatgaaata gctctaaata attcataaca
    59281 aatctcataa gtagatgaaa tataaaaata tcttatggaa ttgtcaaata aaatttctgg
    59341 tggaattact cttagattaa aactttttcg atttgcttta cgatatcatt agtgatggct
    59401 gtaattgatt tattgccatt gatgacgata aaaccataca tatcctgcaa tttgagaaaa
    59461 accttccgta agtactgctg gtaaatcaag aaacttaccg cccagtcgcg cgataagccc
    59521 atatccatcc cggactccca atagtccatt gcgttggctt tttgccagcg gcgctcgatt
    59581 aggcggtgcg gagagacatt caggtaaaaa accaggtcag ggactacagc cacaccgtaa
    59641 atattctgga cccatttaag gtcagtgccg cgcaccagat cgcgcgccat tagggtataa
    59701 aagtaacggt cggcgagaac aatgaagcca gcacgtaacg ccggtatgat gacattttcg
    59761 agctgatcca taaaatcggt agcataaaat agagaaaggg ttgtggggca gagggtattg
    59821 ccgcggcgag ccaactccag ttccggacca accaattgcg agcgagccag ttcgcagttc
    59881 acgaccgcat agcctttgcc ttccagccag gatttaatgc gagtgatctg agtcgtacgg
    59941 ccggagccgt ctgaaccttc caagacgatt aatttaccac tgagtctact gatgtcacat
    60001 tttgggagcg gttcgaggta aaagcttttc attccagttc ccccttttta tacgacgcca
    60061 gatcaatttt ctcggcaata attgcccgaa ttttttcctg ttgggtctta atcggttgag
    60121 tagcatcgat ctcgacgaat ccaaattcct ggctcatctg gtcgtattcg gtaccgattt
    60181 tgccctggta aattcggaag ctatcataga cttcgggatg cagattcaaa tccataccgg
    60241 cttcataata tttaatcttt gggcggttgg agagaatgcg ctcagccgcg atttggggag
    60301 acaatttaaa gtaaaaagta atgtcgggga ttttcgcaaa actatagagg ttgcgcaacc
    60361 aggcgctgga acatccacgc accgcatcac gcgcaaacgc agtgaaaata taacggtcag
    60421 atagagtaat gaagccagtt ttcagaagcg gttgaatttg gcgatgatag cggtcggcaa
    60481 aatctacggc gtgcactaag ctaaatgtgg tcggtgtcaa gaggttcttt ttcttaccac
    60541 gcttgattgc ctgccgaacc atgtccgacg agctccattc cgtcaaataa acatggtgac
    60601 cactgagttc gagccagcgt ttaactaaat agagctgagt tgatttgccg gaaccgtcca
    60661 gcccttcggc ggcaattaaa attccggacg agcgtttaga ttttggataa ttcattaaaa
    60721 tttcgacttt ctaacaaaac ctgactaact ggttcagtta acaagggtaa atttaattaa
    60781 aaaaagaaat aattaaccgc ttttctaaaa tccaaattaa aaagtttcat aaaataaagg
    60841 tggcggcgtc accggtgcct tggaaaaatg ataggcttta cggatggcgc tctgaacatt
    60901 ctggaaagcc ctgcgactgc cggcatgaac ggttacaagc ggttccccaa cttcaacata
    60961 atcgccaatt tttttattaa tgataaaccc gacttccggt ttgatgacgg agtccagcga
    61021 ttgccgaccg gctcccaatg ccacagcgca cataccaatc cgtttcgtgt ctatgttttt
    61081 cacccaaccc gattcggtcg ccagaaccgg cgcctgccag gcggcttgct tatatttctc
    61141 cggatgttcg atgtagctga tatcgccacc ctgtgcttct accattagta agaatttttg
    61201 ataagcctcg cccgaaataa tcagctgttt tagtagttcg cgagcctcac cgaaattctt
    61261 ttctattcca gacagaacga ccatccaagc agttagatcg agcgtaattg tggttaaatc
    61321 ttccggaccc tgacctttga tcgttacgat tgattcgaca acctccagcc aattgccgac
    61381 tgctcgccct aacggctggt tcatatcggt gataaccgct ttgatttgca aaccgaaatt
    61441 ttccccaacc cgtttcagct gtttagctag ttgctgactc tggttgtagt cggctaagaa
    61501 agcgccgtta ccggttttga catccagcac caagccgtca ataccttcgg cgattttctt
    61561 acttaaaata ctgccacaaa tgagtggaat ggaattgacc gtcgcagtta catctctaag
    61621 agcatagatt ttacggtcgg cggggacgag ttcctcggtt tggccgccca tagccagacc
    61681 atatttctcg actaagcgga caaattcctg actgctcaga tttacgttga aacccggaat
    61741 gctttctagc ttatccaaag taccaccagt atgaccgagt ccgcgtccgg aaatcattgg
    61801 cacaacgcac cccgcggcga ctgccagagg cgccagaatc aagcttactt tgtctcccac
    61861 acctccggta ctgtgtttgt ccaccttgca atgtttaata tgactcaaat cgagtttcgt
    61921 accggaaagt aacatcgttt ccactaacgc aaaagtctcg gcgtcggtca ttccttgaaa
    61981 attaaccgcc atcagccaag ccgccatctg gtaatcgggt attttacctt tggtatagga
    62041 cgcgatcaaa tattgaattt cctctttgct atgttccaat ccagcttttt tcttttctat
    62101 cagatccacc ataataaaat ttttggttcg tttcatttgt ttgacagacg gcattacttt
    62161 tctccaggct tatttatgga ttttttttaa tttttttatt caaacggcga aaaagatacg
    62221 ggaaccgtga aaaatctatc acgcgttgct cactggaagt ataatagatc agatatatac
    62281 caacgattaa taggatcaag ttcggcgtcc acatcgccca gaaaggtgaa ataatagccg
    62341 catcagccaa ttcttcgcct ccgatcagga atacatagta caatagaaag aagaaaatac
    62401 tcaaaacggc ggagacggtt atgccacccc gccgggtaat aacgcccagt ggcgcaccca
    62461 gcaggacaaa aacaatgcag gcaaacggaa tcgaatattt cttttgaatt tcgacattat
    62521 atttggcaat ctgtttttta taattgaaag ccagattgct ttcacttcga atgcggttta
    62581 gaaaaatagt caacttctga ttgcggcggg cggcttcggc gggcggcaga ttcttcagat
    62641 tttccgcctg aagagtttga acttttttct cgatcttttt caaatcgaaa gaagtcaggg
    62701 tatcacccag ctccttgcgg actaaattgt taattttggc aatgacttgt tgatattctc
    62761 tggaataacg ctcaacctcg gtgcgcatca ttttgatcgt catctctcga tcgccgcgcc
    62821 gctcactttc gcgtcgttcg agcattaaat tatcaaccgg aatagcaatt cgatgacgct
    62881 caaaatcaat tcgacggtaa tccgatagtc ctttcaaatc aatttcatga atttgaccgg
    62941 agaaaagggt aaatagaatt gtgttgcctt ctactccaat gtatccgcta tcggcataga
    63001 tcgtggtctg aatgtctcgg gcatccttat tataaatagt gactttacgc agactctcgc
    63061 cgactttttc tttaacgtat atggaataat cgggtagatc gtcaatgaag taccctggtt
    63121 caatgttcag atcaggcctt ttgcggtaaa tatcactggt gaggagacgc gctcggtgat
    63181 taaattctgg cagaacacca tcatggaaat acagcatcac tactgatacc accagaccaa
    63241 aaataaatcc cggtacgaga atcgtcgtaa aacttatgcc gctggaacgc atggcggtga
    63301 tttcattatc ctccgccaga cgaccataag ccattaaggt cgctactaaa accgccatcg
    63361 gaactgacat cgctactatc caggctaagt tcagataaag aaattcaaaa atgacaccga
    63421 atggcaaacc ttttcccagc agtcggtcaa ccgatttaat taaaaaatta gccaataaaa
    63481 taaaagtcac aactccgata gaaaataaaa agggaaagat tagttcacga taaatatacc
    63541 gtgctccaat ccaaccggtc gaaaaccaac ataatcggcc tattttctta aaaaatgaac
    63601 gtttcacgag gataatatac ataaccggtc tcatttttta ataaaaaata atttgtatgg
    63661 cagacgaaca tctggcgatt gaattggccg caatttcgac tttttagaga tttactcaaa
    63721 gtaagaacag gaagttacct ttttatcaca ggttgatacc ttgaatttct caaagtataa
    63781 attcatttaa tcattcgatc caaattcggg aaagtatttt aagtattgga atttattatt
    63841 ttttaatggt gaatattttt attgaatccc attattttac tgtttaaact cttctgaggt
    63901 gaatagaata gtatgcgctc caacaagatg agaatatgaa aa
//
LOCUS       DAOOMU010000002        62236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_303_length_62236_cov_17.199997, whole genome shotgun sequence.
ACCESSION   DAOOMU010000002 DAOOMU010000000
VERSION     DAOOMU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 62236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62236
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_303_length_62236_cov_17.199997"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..2421
                     /locus_tag="PLI55_00315"
     CDS             49..2421
                     /locus_tag="PLI55_00315"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPA99313.1"
                     /translation="MKKLFLYCLTGFLLFQSCGFYGRQRGPTDWHRIPKPDYTIPPQA
                     QYIGGLKICLDPGHGGDNHIQNYKRGPTGLREAEINLRVALYLKEFLEKSGATVILTR
                     DGDYEIDLKKRTEMANFNQADIFISLHHNAATNPQTNYASTWYHGDADFAPASLDLAR
                     YIQQSLVENLRLPQQLPTGLLSDYLMHPNGFGVLQNLNMPGVLLEISFFTNPQEEKLL
                     SKPEYNRLEAYAIYLGIVRWAAAGIPKVVLIEPRPDTTIADKQPTIYLKIKDGLHERP
                     GNWMLSREQYFSRQVTFFLDDSIAPFILDRDQNLIIHLPEKPLFNGWHTTHAEVVNYL
                     GNHNLPHRDRFRIAPPAQFLFAKAWNDRLPADGKAFTGIDVLALDIDSLAVADEDTVS
                     AISDYGKWNRQAAATSNGLATFFLQAESQPGTAVLQFSAGEAHTYLTVSFCDTNTTLI
                     AGFCRSAKNSLPIADVLVTLLPDSVQQLTNADGIYYFPHAKPGDYTLNFLKNGYFSQN
                     LEVVVNDGKSNIDTLKMRPIFNGVLHNFVLVLDPRFGGQESGVEISPNLNSAQANLQL
                     AQILKTLFEQAGAEIYLTRDRDSTLSRDQRIKLSNQFPPGGYYLRLNLDCNHQAGRMF
                     KGCHYVGNEEGKRLLQSMANRLTRDGFCDQIQIAGSAEPELVRTNRRALTLDLHLKPQ
                     DSLLISNEASLSRLAWGIFLGFVEELGGENLPTGALTINIRASHKPLADAEIFIGSSL
                     RQLSNQTGAISYEYLEPGKYYLSISHPIYGKREEIFIIQNSSELLINLSE"
     gene            complement(2535..3569)
                     /locus_tag="PLI55_00320"
     CDS             complement(2535..3569)
                     /locus_tag="PLI55_00320"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPA99314.1"
                     /translation="MKNKYFVIGLMILLIVCSHSVFASAPKLGTAGAPELLIPMGAKN
                     VALSGSNIASIAGTDAMYWNPAGMADLKTGEATFTQMSYFADMKVSYLAAGANAGKIG
                     AFGISLQILNIGDIPVTTVNMPEGTGEILSPDYLTLSATYAKRFTDRILFGTNVKLIS
                     EKVGTMSASAVGFDLGLQYISHIGLAFGVTMKNIGSNIEFDGTAIEFDSSVPFANPNA
                     TSRKTKLDMASHELPASMNLGLSYSYKLAELGKINLCGVYSNYSYSYDEMSAGAEFAF
                     RDMLFARAGYLIPLYPSDWEYDETSEYGMSLGFGINLKMAGTNLMFDYAFRPMATFDA
                     NQYFSITIGF"
     gene            complement(3607..6747)
                     /locus_tag="PLI55_00325"
     CDS             complement(3607..6747)
                     /locus_tag="PLI55_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99315.1"
                     /translation="MKKILSIFMIILTVSTLVIGAEDIYKTRALEKAAMTSDIWVNAN
                     RMNGVFRNNGTWFYDNVLGDWGLEWPKGSGLSPIFGAGQYIGAKIDGEIRVAGVQHSA
                     TEFQPGLILATDVADNKADPKYKWYEIRPNGVGDWTNWPTDQGAPLDKDGKPMLIGDQ
                     TIYSVWNDLAPHSEYGTNKLNAEVHQMAWAFSRADAMGDMIFIKWTLVNKSGKNWEDT
                     YFVIWSDPDLGNAGDDQVGCDSTLGLGYCYNATDNDQVYGAAPPAVGIDFFQGPIVPE
                     VGSVVALPDGTVLQDKKMLKMTSFIYYNNDDSNQGNPSSGMDVYNYMSGFWRDGTPIT
                     EGGVGTDPSKPPTKFMFSGDPETGIGWLDSNPADRRFFMTTGPFTMEPWVDSDGDGMA
                     EFGEPGVQEIVAGVIVARGSSNLNSVTTLKEVDNLAQLAYELNFVLAKAPAAPEVTVS
                     ELPNEVVLTWSGNSEYNDDGSLYDSPDPIVAQAFGDTVIIDNTEKVITDSTYNFYGYT
                     VYQYSDASGADPVKVESWDIGKNADPMEYTKQRFIRLLVNKHPKVGNVNDPLINGKEY
                     YFGVVAEGYLEFGAPQIFTSSPTIVKVTPQITAGVRYSASYNDTVEVTHTTLNNATPS
                     DGSTIAWVVDPSRVTGADYSVTFNPDLTWNLIKAPNDTVVANNANQTGNDAYNVVDGL
                     MVKVVGPDPGINLSIPGPFGDMPGYNGFGITGGQRWVSWPTNWGMETFAGAFGNGFTF
                     FGSDLGPADYVDVEIRFAGIGPSGWPQPDSSAAALMEVSKTAYPDRWQKAVVYWRPNY
                     EVQSTLGDVPFTVWDMESDPPRQLKVAFVEDPRPEYDGAANLIWDMGWGDDAKFGVNG
                     GREYVFILNDTYDEQYTDYLNGTLDGTYNNVMYAGGWGARSETRPFLQDAFEFQIYAS
                     NINTVNDVFSFKAPDAANVETAYLKEDLKKINVFPNPYYGYHSGEMNIFERWVRFTFL
                     PEKCTIKIFDLSGHLVRELEKNDATTPFMDWDLKNHYGLPVASSVYIYHVEVPGVGEK
                     VGKLAVFTPNERLDTY"
     gene            complement(6791..9769)
                     /locus_tag="PLI55_00330"
     CDS             complement(6791..9769)
                     /locus_tag="PLI55_00330"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA99316.1"
                     /translation="MARRVAICLLAMLLPIVLFAGTSGKIAGSVVDKNTGAPLAGVNI
                     IVTGGGYNLGAATDLDGYYSILNVPVGTYTVRSSYIGYKEIVYNNVAVSLDLTTELNF
                     EMERTTLEFEQVVVTAERPMIRKDATNTNIIKSAEEIANMPLRGMQEIAASTAGVVKA
                     ENSTAMNIRGGRSAETALYIDGVLVNDPYNNAVYTYLPNEAIAEMSVQTGGFNAEYGD
                     AMSGIVAITTNAGSENYSASFQVITDEFLKADEKILGTYGYGFNEYVASIGGPIIPKS
                     NHTFFISGTRRYAADAWPSYGWAENDYRLDDFTYDQVLTKIDDQGNTFLDTITHNYDL
                     TARIPGNSTSDWSWNAKLKFQLGKNMVLKTSYVSTDRIISSVTPIDIFNTEHMGEESR
                     YSHSFNATLTHTIRQNTYYDLKFNWFDTKRKIYDATFGDDLVKYGDPWLNPWPDDNAY
                     VGVAYTGRITPDYFQPGCQYDDYFKNRTTYWGIDFDIVHQMGKNHTFKAGFEYKYHTL
                     REYRVLAPVYLAKTNLSDLEKYQSADVRFYGYDIDGKEIEEGDFLDDAVYDDAGRLAD
                     GYQKQAPYHPIILSGYIQDKIEFRDLVLNIGLRYDHINPNAWQFKQLDAEWAADGSYI
                     PGTGMFGGNEKFDKTDVEDSEVHTYISPRLGISFPVTDQTVFHAQYGQFYQAPSLQDL
                     YLSPFYLEAFVSRGGYFTTLDNPNLKPPKTTSYEIGFKQMLGNLASVQLTTFYKETED
                     LIQVMNVTTDVTNIAFSRNGDFGTVKGLDIIFNLRRYKNLAATFNYELQYATGTGSAT
                     NSNFNIAWQGGARGNFPKFAMPLDFEQRHTGSLNLDYRLGRTEGPKFLHNAGANLMFS
                     FNSGHPYTLMQVFNTMPFTGRYDNTNVSETPVSAVNSQTTPWVFRVDLKLDKKFYIGG
                     VQLTAYAWALNLLNTENVANVWITTGLPDDTGYLGTSAGRQYWNSLTDEQKSLYKMRE
                     MDYNHYGSPRQIRLGLMLEL"
     gene            complement(9894..10499)
                     /gene="clpP"
                     /locus_tag="PLI55_00335"
     CDS             complement(9894..10499)
                     /gene="clpP"
                     /locus_tag="PLI55_00335"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPA99317.1"
                     /translation="MKTKAQAYVPFVIEQTERGERSYDIYSRLLKERIIFLGLPIDDH
                     VANLVIAQLLFLAAEDAKKDIYLYINSPGGSITAGMAILDTMNYIKPDMVTICLGLAA
                     SMAAVLLASGTRGKRQALPNSRIMIHQPLGGVEGQASDIEIHAREIIRLKENTNRILA
                     ELTGQPLEKINRDTDRNYFMSSEEALKYGIIDEILPARIKK"
     gene            complement(10512..11780)
                     /gene="tig"
                     /locus_tag="PLI55_00340"
     CDS             complement(10512..11780)
                     /gene="tig"
                     /locus_tag="PLI55_00340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPA99318.1"
                     /translation="MKAELINSTPIEKTVKVVVDWDEIADDYIAVFNDLRRDLKIDGF
                     RKGKVPESLAKRLLKPQIFVSFTNQIIEKTHKQALKDVGIDDYLDLHITDLDFDENTP
                     FNYTIKVEYDPEITLPDYKKGIKIQQKEYIVDPETVERYIEDFLENQAEVQEVFDGAQ
                     DGDFLVADLQALDEGGIPIIGKRLPDRLIKVGEGIFGDKEAGNFKGVKAGDSVNLNVK
                     SSSGKMLKYLVDVKRVEKHTLPELTDELVKSQVEDVQTVAEFRQRITENLQYKWNQRA
                     QEELDQALADYFIENTKFDIPPYRINSYLDSLIEKMKENSQNKPIDEAKIREEYLPIA
                     EKNIRWYLIQQAVRKQEDIRVSEDEIQTEIDKILQIVPEEQKEFAEQFYQKSRSRLEI
                     RLNLEDRKIREHIHQFIKEKKVTVHTESLQ"
     gene            complement(11818..11900)
                     /locus_tag="PLI55_00345"
     tRNA            complement(11818..11900)
                     /locus_tag="PLI55_00345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11864..11866),aa:Leu,seq:tag)
     gene            complement(12055..15126)
                     /gene="secA"
                     /locus_tag="PLI55_00350"
     CDS             complement(12055..15126)
                     /gene="secA"
                     /locus_tag="PLI55_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPA99319.1"
                     /translation="MGIATYLTKIFGTSSDREIKKILPLVAEINRIYATLSEQPIEKL
                     RERTAELKVQIQTAREAARIEAEESGISEEEAQKLIQTAEQNALNAILPEAFAMVKEV
                     CRRLVGQEWLVTDQKIVWDMIPYDVQLIGAIVLHQGKIAEMKTGEGKTLVATMPVFLN
                     ALTGRGVHIVTVNDYLAQRDSEWMGRIYTELGLTVGCILNSMDSEERKKMYACDITYG
                     TNNEFGFDYLRDNMALSAEEQVQRSYYYAIVDEVDNILIDEARTPLIISGPVQSSRHE
                     KFDELNPAVGTIVRYQRDKITEILSRFPDSPESIPEKDYSEFARQLFIASQGMPKNRR
                     LRKLLEEPGYQKMLQSGEKEYLLLTNAKTGTSISQDEFFKDLYYFIDEKNNNITLTPT
                     GEEKLATLLGIKLDELIIPDLAEEFVKIDENPELDEVEKEKRKMELDTQHSEISDRYH
                     NISQLLRAYTLFEKDVEYVIQDGKVLIVDEFTGRILPGRRYSDGLHQAIEAKEKVKVE
                     AETQTFATITLQNYFRMYEKLAGMTGTAATESAEFADIYKLEVVVIPTHKPCIRIDYE
                     DQVYKTRREKYNAIINEIETSFRRGQPVLVGTISVEVSETISRMLKRRGIPHNVLNAK
                     NHKREAEIVARAGERGAVTIATNMAGRGTDIKLGQGVVELGGLYIIGTERHESRRIDL
                     QLRGRSGRQGDPGASRFFLSLEDDLMRLFNSERIAAIMDRLGVEEGQVITHPMVTRSI
                     ERAQRRVEERNFTIRKNLLEYDDVMNHQREIIYARRNYALKGSDTKGELYNILEDWAD
                     DLVQKYNAQVKDSGDWQWMALRMDLTTSLGIDLDPSELSEYTPEALKQAIIDRAKQYY
                     ERKEKIYGAEQMRFLERFIMLRTIDEKWRDHLYAMDQLKEGINWRAYGQKDPLLEYKG
                     EAFDAFVQMVNDINQQTLRLCFRAQIGGPQPETRQVRRPSTMRLMHQQADGMGFIPGM
                     QSETEEQPQYATSDGQQAKIRPVRVEAKVGRNDPCPCGSGKKYKYCHGAGN"
     gene            complement(15208..15669)
                     /gene="nusB"
                     /locus_tag="PLI55_00355"
     CDS             complement(15208..15669)
                     /gene="nusB"
                     /locus_tag="PLI55_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPA99320.1"
                     /translation="MRERRAAREVALQALYAEELTSYPLAQIIDDVIENSELQDELKT
                     FAKDLFIAAVTHRDELDEIIREKSENWDFERIAIIDRLVIRMAISEFKYFNDIPPKVS
                     ISEAIEIAKIYSTDDSSAFVNGILDAVLHDISDTLTYKLKRSETKSATREE"
     gene            16049..18415
                     /locus_tag="PLI55_00360"
     CDS             16049..18415
                     /locus_tag="PLI55_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPA99321.1"
                     /translation="MPPKKIDNTQIPTSNVEKYYLSLSGRQFIYPPTEKIPVIQVANF
                     PMLGQLTALRFLEWVQNNPDGVIALPTGKTPEHFIKWVGYYLTNWHSAEVIKDLEMVE
                     IDPARRPDMARLHFVQIDEFYPIDPNQQNSFYYYIQKYYIKNFGLDPEKALLINLYEI
                     GTANNLSIAEIFGDEKADLSLRTRWAKNPIEELQKETIARTDQFCVNYESKIRALGGI
                     GFFLGGIGPDGHIAFNIRGSDHYSTTRLMETNYETQAAAASDLGGIEIARKRLVITIG
                     LDTITYNKDATAIIIAAGQAKANIVRDSIQNFPSIQYPATVLQKLPNARFYLTEGAAS
                     KLFERRYEKMAKSPELEPKTIEKAVINLTLEKNKKLTDLTLADFNSNRFTELILKRTN
                     QTHTEICRNLHESIINRIDRGIYYLDNEVFMHTSPHHDDEMLGYLPYIVHLVRNPRNK
                     HYFTYMTSGFTAVTNSYVLERLKNLQDLVDSPTFQVLMQEHYFDPANTAARNRDVNLY
                     LDGVALYDVKKKTEAESRRLLSILTEIYEENNVIYLKDRISELINYCATQYPGKKDIA
                     HIQKLKGMIREWEADVLWGYFGFNSNNVFHLRLGFYKGDIFSEEPTLDRDVLPIYQLM
                     KKIRPTVITVALDPEGSGPDTHYKVLQAITEALKLYEKEEDISNLRIWGYRNVWHRFH
                     PASTNIYVPVSLNSMAIMENAFMESFGSQRSASFPSWEFDGPFCRLARKIQVDQYLKI
                     KTCLGRDFFIKNEHPRLRATHGFVFIKELTPKEFYRHSEESRRVTENL"
     gene            18566..18805
                     /locus_tag="PLI55_00365"
     CDS             18566..18805
                     /locus_tag="PLI55_00365"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99322.1"
                     /translation="MFCVLFENFIGFNIFNMLLIMVVAGLIAPINGLGIKSFELLFMA
                     ILSLAIYPITLKGLKINRFEDLFLLLLYGGYLYKN"
     gene            18830..20263
                     /locus_tag="PLI55_00370"
     CDS             18830..20263
                     /locus_tag="PLI55_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPA99323.1"
                     /translation="MHRFQLFSLIIFWASISNCSNPISPKPENPFKNINLQILSDSTL
                     AITWQGEFESEALLSLDRKVGEASWQMDYTNVPANDSIYVDTLYSVHTDTVYSYRLRF
                     KESDNSSSISPSVALLSERCVPVDFSARQIDKSTIRIAWRDQSFGEAGFRIDRQVGND
                     DWQIAYAKADSNSYEYFDITTTSSEISYRIAAYAGSSFSSFTEIAKIKLGSLTLANLY
                     FGTEPTFEVMTWNIEEFPRFDTLTVSAVSQAIKALDIDIIGMQEIKSGTAFQQLLDSL
                     DTWDGYRTNSAAYGINLAFVYNPANINVNQYYEILEHEDAFPRPPLVIELEWQNVPII
                     VINNHFKAMGDEDSQNHRLQACTLLEEYIRTNLADDNVIVIGDLNDELTDAQSVNVFG
                     PFLSQPSAYKFADYSIAQGSSKYWSYPTWPSHIDHILITDELFGVFANSASVIKTLLI
                     ENYMEGGWNEYAVKISDHRPVALKLAF"
     gene            20352..20693
                     /locus_tag="PLI55_00375"
     CDS             20352..20693
                     /locus_tag="PLI55_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99324.1"
                     /translation="MLNIIKSFVAILKIEIEDLAADVELMMNECQKKKDAGKITDYVY
                     LENMALYRHELLAVRSFARLLESTELSNFETLDALITYLQESFREAGYVRAINICVER
                     KMKKVAGYVSR"
     gene            20932..22017
                     /locus_tag="PLI55_00380"
     CDS             20932..22017
                     /locus_tag="PLI55_00380"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPA99325.1"
                     /translation="MKLLDRYILKKFLLIFGLCLLAFLVIFNIVDLIEKLDKFLKADM
                     PSNLIALFYLYQLPYYLNLALPMALLLAAVFTIGLLSKHNELDAIKSSGISLYRVSIP
                     LLIAGILISIGSFFFEDAVVIPATRRRIDIEAHNMRRRKYIPKTFFTNIMYQDSPNCN
                     IVIGNFSTKDNIAQNVTIQYNDQFRLVRRIDARKMVWLPEQQLWQLFDYKVRSFDQDG
                     TEIVAAASSDTLIQLNLQPEDIVKSDLNPEGMRYRELAYFIKRLQESGNDARKWEVNK
                     HFKLAFPFTNFIVILFGVPLAAAKPHKGIAFGAGMSLLAIFIYYGFIKLGQVLGYKGI
                     LSPLLAVWLGNIVFLIAGGYLLYKIRQ"
     gene            complement(22254..23624)
                     /locus_tag="PLI55_00385"
     CDS             complement(22254..23624)
                     /locus_tag="PLI55_00385"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPA99326.1"
                     /translation="MAKKKMGWLNNRYKITLLLMLGMSIMATAQTRRDPRALALAGAY
                     GSLARGIFAIDYNPANLAIPHEYHSYRIFGGFGTNVSTNFLSIKQYQKYNGKDLEAGN
                     GHLKKEFMNDIPDEGWRVFTDIYFPLPLISYSTYNRAISSDLIVIGDLGLPKDLLNFV
                     FDENPIGETLDLDFNEEVMAVIQWGYSLAFPVGNIFLGFTAKYLQGLAYIGLNPDSSY
                     GSVTTYFEPERTYIDGYGQYYFQQSLGGRGFALDIGLTTEELNGYRLGLSLTNLFGSI
                     TWNKSTLLSRSVSVEKVLPWEGNFYKYVFSIDEARFDKFFGKASVDDVFHGEGYKIKD
                     TTAFRVKYPSLVRFSAAKILEENITLASDLVVGFEDRLYSFGAWKWCLGLESTKSQRF
                     PLRVGLSFGGHSSRELTFGSGFHWGLIHLDWALGFNHGLWPTTTTGLNFNMLCYSTKR
                     ANTSYR"
     gene            complement(23652..25910)
                     /locus_tag="PLI55_00390"
     CDS             complement(23652..25910)
                     /locus_tag="PLI55_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99327.1"
                     /translation="MIRILSFIKGLFLLKHFRLYSTIFFLSIGLICGCSYEPLAPPEM
                     PTYYQTINLPLADVSLPLADLVNPDNNIYGDSTKDQLYFQFSGNLDTVTLTETVFQIP
                     TVGNVNFSQNFSELSESQLTFSRSVTQTTKLSEIITIPGTLPSPNELLIGEIARYELD
                     GQRYKYRVFDKDDVPYFERVDYLTVGSGTFQTEIDNQLPVDMDSVRITLRNKNGSLIA
                     ESFYENVPAGATLSDGTPGNLNGAQLFDSIEVIVTAVMAGSQGQPVTIPANTDPFIAI
                     KFDTNIEEIESFTGIPKPIVTTQNQLLPPSNNTIFKATIAQTTTSPLDTNFLDLTIHN
                     LMPFNLRMQVIFHNFYIRNEPLTIDTIVQSGLTVENPARLDGYIFRNPDPTTVVDSFL
                     VEIIATILPNAGEQVVTIPTDMGDASIDVNLYFARLKFDMLEGFFNESFDIPPMAIAN
                     IPTGLANVDFGQVFLKLTFFNEIQARTNVNLVLRGLKKGVAPQTITEDGSIAKASPAN
                     PIQESTMEIDIAPIFNMVPDSILVSGQAIIPSDDTSRLQIGKAFWGFYEITVPFQVKI
                     GQVTFIPVNSTEMAAIDPETRQKIRDGLIKSSIYTEIINDFPFSGNLSILMSNYDYFP
                     LEPDNLDAGYFWLNDTLYARTDTGDVPIIIDTLLLVELPPPEAFNSNGSVKTPGFTLA
                     ETPLDSAKMEAILRDETHYIRPRVHFNATEDFVIVGYYDEVRILAAISLTVETMELIN
                     NNNENETPATVP"
     gene            complement(25926..>26896)
                     /locus_tag="PLI55_00395"
     CDS             complement(25926..>26896)
                     /locus_tag="PLI55_00395"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPA99328.1"
                     /translation="SKPALDVIFGNWEKGTTYNLDLRCDAMTAVEYVYSIGIPFGKMS
                     VGISLKYLQGLGYYGLDPAYSTGQIAVDTSDFVLYGNGSYYFRESSHGRGYGADLGVA
                     FEDLNGWTLGFSVLNLGESVKWNTETALSKWLKDNGIDVLKMMGFQIKSFPVKNTDLK
                     LDFEGESYFYNFRIDSLDADALFRSENTYQDHFSDTKLIKQDSSAFRVQVPLVLKVGA
                     AKQLRKDLLTAIDFSMSFSDRLNLTKGWRTAVGFEYAYFPKMPLRVGLALGGISGWEF
                     DIGSSVNLAALHIDWALGMNRGLWVHSMQGFDFALATYLVRGGKKK"
     assembly_gap    26897..26996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(27169..28689)
                     /locus_tag="PLI55_00400"
     CDS             complement(27169..28689)
                     /locus_tag="PLI55_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99329.1"
                     /translation="MLDTENGNPIKADQSGVLYYSFTDSTSMPIEINPTTFRIVPAMT
                     ISQTLDLRKNIKRNVNNTVNPINYIMKKAWDDSNNRVTSGVLDNSTGFTADNIPINAL
                     VLAAQLSDSFPNPIQIRVIAPNFQKNPGQSVVATLDLPVNSLQNSIMIELGGASLIGG
                     LLKEEIDSLEFNIEVQVLGELAEATSSLKQKLTTSLTVGQLALESFNGRVFATGETPP
                     ALVAKSLTGASDIEFDSARASLNIETFGRDFDSVYVHLSGKKVHGVETVSDTMIAINR
                     ADLKMDIGEVVSNLPDTVRVFVKVMTATADFERNTVFNAGVNVKYNISVPLRFKIPPQ
                     ITLASGNTTTYFIKDSTTRARLITSQNGGELDMTVENYTQLQGDLYFLISNFNFFPFD
                     TLGGNLPSDFVIFDGTICHLGDDTTVVEIDTLAVLEFPSAQFSENTLIIPGIKSQIFQ
                     ISSDAIGLLADTCYFKPYFKLVNPDTSLATITATQSIHVRGFLDLYFVAANLKDSD"
     gene            complement(28849..30102)
                     /locus_tag="PLI55_00405"
     CDS             complement(28849..30102)
                     /locus_tag="PLI55_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPA99330.1"
                     /translation="MTGKIKDITLAPSGKLKIEWAESHMPVMMALRQKYRDSKPLKGM
                     RISGCLHVTKETAVLIRTLIAAGAEVAWSGCNPLSTQDDVAAALYEEGVPIWAWHGLS
                     TAEFYWCIEQTLLQKPNLTLDDGADLVFTIHNKFPELAAGVLGGSEETTTGVHRLRAM
                     AEDGKLLYPMVAVNDAETKWDFDNVYGTGQSSIDGILRATSILYAGKTMVVAGYGHCG
                     KGVAKRASGMGANVIVTEVKPTAALKAKLDGFRVMPMDQAAELGDIFITATGMKDVIV
                     SRHFEKMKDGAIVCNTGHYDCEINLKELATLTKTKQEMRPNCVKHELTNGRSIYVLGE
                     GRLVNLVAAEGHPSEVMDMSFANQFMTMIFLAQNGAKLAKKVYDISVEQDQEIALLKL
                     QTMGVNIDTLTPEQVRYKTAYSEGT"
     gene            complement(30102..31259)
                     /gene="metK"
                     /locus_tag="PLI55_00410"
     CDS             complement(30102..31259)
                     /gene="metK"
                     /locus_tag="PLI55_00410"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017186814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPA99331.1"
                     /translation="MKKYSFTSESVTEGHPDKICDQVSDAILDALLTVDPESRVACET
                     MTTTGLVMVVGEITTKAYVDIPRIVRETIEKIGYTDPDFGLDARSCAVITSIHEQSEN
                     IALGVNETEKHEQGAGDQGLMFGYACDETPELMPLPISMANKLTMRLAEVRKKGILNW
                     VRPDGKSQVTVEYEDDHPKRITNVVVAVHHNPEIAHEKIVADIKNHVILPVCQGYLTP
                     ETKFYINATGIFAIGGPEADSGVTGRKIICDTYGGMGRHGGGAFSGKDPSKVDRSASY
                     MARYIAKNIVAAGLARRCEVQLAYSIGVADPISVYINTSGTGKQPEERLAEAVRAIFP
                     LKPAAIIKYLCLKRPIYLKTAAYGHFGREDEEFSWEKTDKVAELKQYFGVK"
     gene            complement(31321..32295)
                     /locus_tag="PLI55_00415"
     CDS             complement(31321..32295)
                     /locus_tag="PLI55_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPA99332.1"
                     /translation="MKAIIPAAGIGERLRPLTLTRSKVLLPVAGKPIIGHIYDRLALT
                     GIKDVTVIIGYYGEQVRDYSLKNYDFNFTFIEQLEQCGLGHAVGLGLTPEDGPVMIIL
                     GDVILELDFKGLSSRSENLIGVMPVDDPRRFGIVEMKDGKVTRLVEKPSSSTSNLAIA
                     GIYLIQRGALLKKAIDYIITVNIKTKGEYQLTDALQKMLEWGESFEVINIEHNLDCGT
                     RKSLLKTNAYLLNKNPQPPPAIPETVIIPPVYIGRDAVIKRSIIGPNVHLGAEVQVMN
                     SVLSNAIISDGAILKDVILDESLIGQKSQVSGIVQQIDTADYTVQKLS"
     gene            complement(32311..33294)
                     /gene="hprK"
                     /locus_tag="PLI55_00420"
     CDS             complement(32311..33294)
                     /gene="hprK"
                     /locus_tag="PLI55_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HPA99333.1"
                     /translation="MNQNDLTVGRIYHENRKKLGFLRINGDIGHDTVVTSSKLNRPGL
                     EISGFWESFDPHRIQLIGEGEFRYIKTLSGEKVEEIFEHFFSAKIPLMIFAHDVKPPV
                     CAVRLARKYQVALLATPVTTTLLVASLLDYLDRELAPSTIIHGSLVDVYGIGLLLTGP
                     SGIGKSEIALDLVGRGHRLVADDVVKIIRRADNIIIGSGVEILEHHLELRGLGIVDVR
                     NIYGIRAIRQQKRIEVQIELEEWDNTKNFERIGAQEKYNKILDVDIPIINLPIYPGKN
                     ITVIAEVIALNHVLKIMGTNAALNFQKKIAATIRQKTQISDLQNYLKKDLE"
     gene            complement(33291..33578)
                     /gene="raiA"
                     /locus_tag="PLI55_00425"
     CDS             complement(33291..33578)
                     /gene="raiA"
                     /locus_tag="PLI55_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPA99334.1"
                     /translation="MNIELTTRHYEITDRAKKHLEEEMNNLKLYEDLITSCKVIMEKT
                     KDGESIEISIHVRGKDLIAQVASDDMIKSIDLAIAKIERQLKKFKEKRYAR"
     gene            complement(33698..34645)
                     /locus_tag="PLI55_00430"
     CDS             complement(33698..34645)
                     /locus_tag="PLI55_00430"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HPA99335.1"
                     /translation="MITVEEMMINFLRFLAQERGYSENTIMAYRRDLEQFFLFYAEYA
                     NQVPIDLNSVSKMAIRHYLGKLSESGLEISSISRKLASLKAFFKFATRQGYVDSNPAA
                     LILSPKAKKRLPVVLTEEEIAKAIELPDDNTLVGKRNRAILELFYSSGIRLGELIALN
                     VGDLNFSRQTLRVFGKGAKERVVPFGKKAAEVINDYLNMRKKEVGALELNSPLFISSS
                     GGPNSQPTGYRRISRSQVQKLISGLLQQASEQTHLSPHVLRHSFASHLLDHGADLNAV
                     KDLLGHSSLSTTQLYTHVRISTMKEVYQQAHPHADSGKK"
     gene            complement(34642..>35547)
                     /locus_tag="PLI55_00435"
     CDS             complement(34642..>35547)
                     /locus_tag="PLI55_00435"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPA99336.1"
                     /translation="SEKLPAFSRTKIAGLIKNGKILVNGQISKPSYHLQAMDTIAVFA
                     EPQPISPIQPEDIPLEILYEDEYLLAINKKPGIVVHPGAGVRQGTLVSALLHYTNQLS
                     SLGGSERPGLVHRLDKDTSGVLIVAKNDEVHWKMSRLFAERKVFKQYRAIVYGVPETA
                     VGIIDAPLRRSQRNRQIFTIDKRGRPARTQFTILKDYRIMAYLAVVLETGRTHQARVH
                     LQSIGHPVVGDPVYGGKLRQGSAVSAKDVSLLKKVQSEVHRQLLHAYLVRFQHPFFDK
                     DIEIIAPLPDDFSRILNILEAGIEL"
     assembly_gap    35548..35647
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<35648..36015)
                     /locus_tag="PLI55_00440"
     CDS             complement(<35648..36015)
                     /locus_tag="PLI55_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPA99337.1"
                     /translation="MEAKTTKKAWSKEDLEYFKNLILKKREETLKAIERLEATTVSDN
                     TRNDNTAMDSAYAYHMADVGTDSQEREKAFLWLARENKFLSHLNSALERIKTGEYGVC
                     IECGELIMKERLEEVPHTQH"
     gene            complement(36042..39182)
                     /gene="ileS"
                     /locus_tag="PLI55_00445"
     CDS             complement(36042..39182)
                     /gene="ileS"
                     /locus_tag="PLI55_00445"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPA99338.1"
                     /translation="MIKKVPSSVDFVAIEHEILKFWEQNQIFKKLVAQNANGPKWSFI
                     DGPITANNPMGVHHAWGRTYKDIFHRYKAMNGFRTRYQNGFDCQGLWVEVEVEKELGF
                     KSKRDIEKYGIEKFVERCKERVRKYSKIQTEQSIRLGYWMDWKNSYYTMSDENNYTIW
                     LFLKKCHENGWIYKGHDVMPWCTSCGAALSEHEIATEGYKEITHPSITVKFPLINRPG
                     ESLLVWTTTPWTLTSNTAAAVHPDLNYLKIRQGNEILYVVEGRERDVIKGEFTVEGKI
                     PGREMLGWTYHGPFDDLPAQKNVKHVVIDWEEISASEGTGIVHIAPGCGQEDHELGKI
                     HDLAVLAPLDEFGNYLEGFGWLTGRNVDGISRDIFKDLEAKGIKYRTDSYLHRYPVCW
                     RHGTELIFRLVDEWFIAMDGVRQPMMDITRKIRWIPEYGLQHELDWLRNMHDWMISKK
                     RYWGLALPIYECQDCGQVTVIGSREELKEKAMEGWDKFEGHSPHRPWVDNVQITCPRC
                     GAKVSRIPDVGNPWLDAGIVPFSTLHYTTDRAYWEEWFPADFICESLPGQFRNWFYSL
                     LAMSTVLENREPFKTILGYALVKDEKGQDMHKSAGNAIWFDEAAEKMGVDVMRWLYAD
                     HNPFNNLNFGYNIAQDYRKRLITLWNSYSFFATYAELDGYSPQKHQVSDENLSELDRW
                     LMARLVQLIRLSREALDDYNVARVMKGVDDFLEVLSNWYIRRSRRRFWKSDNDQDKWS
                     AYQTLYKALKGLILILAPVVPFITEKIYQNLVRGLEPDAPESIHLAKFPQAEEEFDEA
                     LLKKIDTIIEIVSVGRAARNKANLKVRQPLAQMWVKLPAEVTANDLVDLTDQILEELN
                     VKALDFITDETQFVNIQIKPNYAQLGAKYGQRLPAIRQAIAELNPIEVARRLEKSENL
                     ELNLIDGKIWLSRDEVIVERKENEGFAIAESNGITVILDTKLTPSLLQEGLVRDFIRH
                     VQTMRKEADFQVEDRIQVNFEAPADFTAAVHNYIEYFCNETLAQKVVDNFAPGEYEKE
                     LKIENNKVRVSLTRIK"
     gene            complement(39197..39418)
                     /locus_tag="PLI55_00450"
     CDS             complement(39197..39418)
                     /locus_tag="PLI55_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HPA99339.1"
                     /translation="MNQVGKLLIIFGVVLIVVGVLLTGAGKFPFLDKIGRLPGDIIIR
                     REHFTFYFPWVTCLLISLVFFILFNLFRR"
     gene            complement(39593..41092)
                     /locus_tag="PLI55_00455"
     CDS             complement(39593..41092)
                     /locus_tag="PLI55_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HPA99340.1"
                     /translation="MKRKIGIVIGGILLVALGFTIASIIIRPGKVVFYEDKNSGMTTF
                     RPAAVTAGEDIPSLQAFNDAFVQIAESVTPSVVTITTEKVIKTNLRRDFPGFPDDDLF
                     WRFFGFPQGEMRSRALGSGVIVDQKGYILTNNHVVERGEKISVKLIDNREFKAEIVGT
                     DPLTDLAVIKIDAKDLQPAILGDSDALRVGEWVLAIGSPFSENLAHTVTAGIVSAKGR
                     SGVINNTNYESFIQTDAAINPGNSGGALVNIRAELIGINTAIATAGMSQGNVGIGFAI
                     PINLAKKVMTDLIEKGKVVRAWLGVVIGDVDENIAKSLGIESRQGAIISEVVKDSPAE
                     KAGLKVGDVIKEFDGKTIKDSAHLRTLVSTSEVGKPKTLVVLRGKKELKIDVKLGELP
                     ESVERGVYPDTKSDSKLGIRVDEINSVTASQYGISPDETGVVVTGVDRNGRAAGILQV
                     GDIIKRIGERDISNVAEYQKALEEVKTDIVLILIKRKNVTFFETIDLSK"
     gene            41545..42822
                     /locus_tag="PLI55_00460"
     CDS             41545..42822
                     /locus_tag="PLI55_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPA99341.1"
                     /translation="MSRINPGNKKIPKVIVVGGGPAGLMAAGQASLSGAQVILIEKME
                     RPGRKLAITGKGRCNLTNTTELSEFLTHFGRNSRFLRGAFHRFFVPELLEFFKQLGIP
                     TVTERGGRIFPVRNDANEIVTALVNWANSTGVIIRPNTRANQLVLTENRISGVIVRNP
                     NSTTEKINADAVIIATGGASYPGTGSTGDGYRLAKSAGHSITPIRPALVPLVTVGNLA
                     TRLQGLSLKNVGIKVFADSRKIADEFGEMLFTHFGLSGPVILTVSKLVVDALNAKQKV
                     RISIDLKPALDDQKLDLRLQRDFAQHGKMQFKSVLKEFLPQKLIPVCLDLIKIPPEKT
                     CNQITTAERRRLRLWLKNFNFDISGHRGFGEAIITAGGVALNEVDSQTLQSKIVQGLY
                     FAGEILDLDADTGGYNLQAAFSTGWLAGQSAGR"
     gene            43024..44736
                     /locus_tag="PLI55_00465"
     CDS             43024..44736
                     /locus_tag="PLI55_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPA99342.1"
                     /translation="MFLPTTKQEMEELGWQELDVILVTGDAYIDSPHIGIAVIGKVLL
                     KAGYRVGVIAQPALNVPDDIMRLGEPCLFWGVTGGSIDSMVANYTASLKKRRHDDYTP
                     GGINDRRPDRAVIVYTNLIRQHFKKTKPIVLGGIEASLRRIAHYDYWSDSIRRSILFD
                     AKADFITYGMSERSILELAESLQKGSDYRDIRGICYIAPHKPENTLELPSYEKVRDNP
                     KTFTRMFHEFYGNNDARSAQRLCQKYATRWLVHNPPSLPLSQAELDEVYNLNYERDVH
                     PYYAKQGSVKALETIQFAVTTHRGCYGECNFCAIAIHEGRTVQWRSPESVTNEVRSFA
                     TMPNFKGIVADVGGPTANMYGFECPQKLKTGGCKQKRCIYPEICPQLPVSHKKQIELL
                     IKLRALPGVRKVFVASGIRHDLVLADKKWGRKYLREVISEHTSGQMKIAPEHIVPQVL
                     RLMGKPNHETLINFIKLFYNYTRAANKKQFLTYYLIAAHPGCTYQDMVKLKSFATNFM
                     KITPEQVQIFLPAPSTYSALMYHTGFDPFTAEKIFVAKSLHQKEIQKNVVTSKMKNPI
                     LKSK"
     gene            44745..46106
                     /locus_tag="PLI55_00470"
     CDS             44745..46106
                     /locus_tag="PLI55_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HPA99343.1"
                     /translation="MTDKRLEEYRQALIDLSNGKTPHPINCEPDDELSALGREIIRLQ
                     KEWQELSLLIGLTAQINAGLNLEEVLDRIFDSFHAIIPYDRIGVALLTDDGKNVRSCC
                     TRSRLPVIKLKEGYSARLKNSSLEQIIRTGQPRIINDLEEYLAQKPRSTSTRLILSEG
                     MLSSLTCPLIIQTKPVGFIFFSSCNKNTYRDAHIELFQKIADQVSVIIEKSRLYQNLA
                     ALNEEKTKFLGIAAHDLRSPIGVIKSYTDILLAGLVGNLTDQQTNIIKTIENNCTRML
                     NLINDLLDISAIESGKLTLQKTTIEPSIFFANYAENAMRLAAVKSIHFTTFIAPQIPP
                     IEFDPNRITQVLDNLVSNAIKFSSPETTITLAVKAIGKNLEIRVADQGQGIPAAEIPK
                     LFKPFSRTSVQPTGGEKSTGLGLAIVRRIVQAHGGKIRVKSQPGQGTEFIIHLPFISK
                     SDN"
     gene            46304..47071
                     /locus_tag="PLI55_00475"
     CDS             46304..47071
                     /locus_tag="PLI55_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPA99344.1"
                     /translation="MRELKFKPGDIIINEGAYGSEAYILKAGSVEVSKMTDHKKVILA
                     ILQPQEIFGELGLIDDKPRSATVIARTAVTVDEITREDFSALLDDKMPFVIPIMRSFF
                     EHLRQANQLVLQMEAQITEASKTAPQPNAPTEIKLEGLTPIASQSLKNRTLILRKFPF
                     KIGRESGQRREDVFIDNDLSLIDNIPYNVSRNHMSINFYNGQYYILDRGSSLGTIVNK
                     IPIGGRLSNFKAELHKGENTVILGTSNSQFQFKITIE"
     gene            47068..>48144
                     /locus_tag="PLI55_00480"
     CDS             47068..>48144
                     /locus_tag="PLI55_00480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA99345.1"
                     /translation="MIPRKNLARAFQKAIEQPDYALNNFIHRLKASFSYHFLNGNSLG
                     PETISLFLTYKCNLKCAMCGQWGTNGAFREYPSEVVRQQLSLDEIQNLIDDVRHYNPN
                     ITLFGGEPMLYPNWLKVVEIVKAAGLRCNIVTNGTYIGKWVREIADSRLDEIIFSLDG
                     PAEIHDKIRGVPGTFQKAINGLQELNTLKKTRNLNRPLVNINCTITELNYRSIDEIIK
                     IAEDIGACGLTLHHLLFLDRATVMEFIPFFQEKFGQTPTDWLGFIVDELPQIDPEYLI
                     AKRNELKTRRFDTDVNFYPNLEDNEIRQWYTQFQFKASSYKNRCQSLWATAYVFPDGS
                     VRPYHSMNFTPGNILQDKFSKIWNN"
     assembly_gap    48145..48244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            48461..48847
                     /locus_tag="PLI55_00485"
     CDS             48461..48847
                     /locus_tag="PLI55_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA99346.1"
                     /translation="MEKILIVDDEQEIAELLEEVLEQLGYKTAMAFSKAQASEVLREF
                     EPRIVLLDIRLPDGDGISFLKEIKASQPAVEVIMITGLADRDIAIEALREGAADYICK
                     PIDLDYLANSLLANIVTRYNTDAPAL"
     gene            48902..50149
                     /locus_tag="PLI55_00490"
     CDS             48902..50149
                     /locus_tag="PLI55_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPA99347.1"
                     /translation="MKRNQFLELPKKIILLVCITSLFSFAQKREPSITTDSYQNEMTL
                     SPGDRIKVSYIDIGPQGVPIEKSSILEVRHDGTIFHELLKVVYVQNLTIKQVEDLLTV
                     KFSEFFTQPQVVVSIVEKTTIKVFLWGGVDNIGVYRISPGTTLAEFIITHGGLTSDAN
                     ISKITLTRKDGSVIIFDLERYLFSNDETNNVELHDGDKIIVPRLIMKDEYARLSKGYI
                     LQAGNVLEITINELATMEKSPATPENYIIDQDGNIYHRRFGLVQLGGLTVDKAQATLT
                     ELAKKYYREPVVTIDVVKLTSRSVFVFGEVNRPGIYPIEGNIRLAEFLADIGGLTSNA
                     DPRKIIVTRENYKPVTFDLNEYLFDRKDEKNIYLEDGDRIIVLSRKRGFIVNLAEKIQ
                     PFTTVLYIISTALNIYFLITYIK"
     gene            50163..53351
                     /locus_tag="PLI55_00495"
     CDS             50163..53351
                     /locus_tag="PLI55_00495"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPA99348.1"
                     /translation="MAHYELNLRDYWRIIKKRKIIIIVTLVLLTCFSLIFAIMNRPTP
                     LYRATASLKIEKSTNLTGLYMYSISWGTGDDLATRAEEIKSYPMIEKAAQKLGLIDSS
                     LTSEQIRSNKEYLDLVSRLKSRVKTEQEGYTNIINIIVTDYDPVLAANLANALAQVYE
                     VESFKEKNLQAQKALEAVRNQRAMAEQALRISEQKVQNYREQNKLATLDGAASRLTAE
                     LNSAETNLEKIRGDIHQIDNILREIGNNREYIYLANLNLLLSQPNRFLEDLQTQLNQI
                     RSQITNYSQYYTPQHPAIIELQQQLKNTENRFIHELEAFRQNLARAETIAEEKYKRLN
                     EEYKSLPLIELTLTNLERERDVNTQIYQQLEIRYQEAIIRYSEQVKEVFIIRPAFVPT
                     SPINQTTVGPTTAIGMIIGLILGIVLAFVAETLDTTFNTVDDIKEALNTNIMGIIPFV
                     DFQGLKSQLAEKAETPVPDEILDMQARLVSYYSPKSSIAESFRSLRTNVHFALLDKGY
                     KTLMITSSISNEGKTSIAANLAISMAQIGINTLLVDADLRKPRISKLFGIDREPGLTD
                     VILRKEPIDNVIRTMSDLMVGTMACDILRTDNIAGIEYLNILTSGKIERNPAEIAASK
                     LMDNLIAELKRRYDLVIFDTSPLIPATDSTILGTKVDTVLLVYYQGKISRGTLRRSKS
                     QLELLKTDILGVVVNGMKAELSADYADYKYGYEYHYQEESARPKNKVITALESFFLKP
                     AVGMTNGFFSQLNKLRAGLALIALGLIIGGGLWAFTKYQGKKAKSQVESKITETIIPQ
                     TTSSSDTSVASHILSSDTLSNLTAQTAPIIPSELKQLQEQYGIETISQQQVDQNLTES
                     GLLTNNSPTTVQSTIPPATQSDKQTEPLISIVYSSTILSKTDPAASVQPLPSSLPYTL
                     IIEQSFDKSTLINQLNKLRRNGLSAFITIDFSDPKQKNYLLCYGSFSSENEAINKSKE
                     LQFLGFRGTFRTIKLPFSILLDDFPTLQSAQQAIERFASIKDYLYIQPSSQKPDAKKT
                     YYLLFGGFPSTESTELVMNTYPELGHKTIVIR"
     gene            53363..53710
                     /locus_tag="PLI55_00500"
     CDS             53363..53710
                     /locus_tag="PLI55_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99349.1"
                     /translation="MPRHKHHKSRSNRKAIIFLVIPISLIIAITIIIIIREPNWPFLS
                     ILRQDETANQEKYMGSAVRVETPIRQKYETEWRNDYFETWQKTYEKILQNTDKNEIKE
                     LERELKASEKGIN"
     gene            53781..55139
                     /locus_tag="PLI55_00505"
     CDS             53781..55139
                     /locus_tag="PLI55_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPA99350.1"
                     /translation="MLVALVLVKVDPVYAILAAVGLGILIVTFTNTDFAIYILIFSML
                     LSPEIGSRTTSGEGMTIRIDDLILAIIILTWLAKTAINKELGLFRKTPLNKPIWIYTL
                     VCILSTGIGMMAGRVNFLTGLLFVLKYIQYFMIYFMVMNQIRTKKQINNYLISLIITF
                     TIVTIFALIQAPSGQRITAPFEGEAGEPNTLGGYLVLLIAINLGLVLNDDAIANRHLR
                     RGLLLITMISIYPLLMTYSRGSWLAALAVIIAYIAISKKRWIIFGVVVLVFVLAPYIF
                     PQSVIDRVKYTFTEQQGYDATLQEHIGGLTIDTSASERIRSWRAAFRDITKRPVLGFG
                     ITGWRFLDAQYIRVLIETGIIGLAAFVYLQFSLLSNIRKVYSDAKTPLYRALAQGFFV
                     GMIGMMAHSITANTFIIVRIIEPFWLACGLVMAVPEVEELEAEKLPEIEKSPTTINLE
                     NR"
     gene            55145..55426
                     /locus_tag="PLI55_00510"
     CDS             55145..55426
                     /locus_tag="PLI55_00510"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPA99351.1"
                     /translation="MGKIDLDFKPTTRPDLPFRELKDGGVVYEYRTDTLHSLNATAAY
                     IWVLCDGQHTVAEIISSIKQNFNRFEVDPTGEVIKIIEKFRTLNLLTDD"
     gene            55426..56274
                     /locus_tag="PLI55_00515"
     CDS             55426..56274
                     /locus_tag="PLI55_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99352.1"
                     /translation="MPFFKIQVGSAEINLECCQSIISEVTASVFSAILTPSQISAKIH
                     YRIETKNSKYFLFRNNRLTHRNRQLSRIIYALEWQIVQDLLTKNKSVLKFHGAALKHL
                     DTGFIFLGAASSGKTSLAILLMRYGWEFFSDEFALVDCDRQIIPFPRNLIIKPHLRDK
                     INIPLDYPIFKLDDESDGKIDAHYISPSLFGTVYLSKIPLKGLVFLEKADRAGFDLQP
                     LEHHKAFRLLTQHLLNRNFSGEKWLDFLVNLLGSSAVYRLKISSPMDLTPSDSRALVE
                     QLNQIE"
     gene            56276..57409
                     /locus_tag="PLI55_00520"
     CDS             56276..57409
                     /locus_tag="PLI55_00520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA99353.1"
                     /translation="MIKYFRDLLSDRIRIENFRQAIESTVNHNSIVVEIGSALGTYSF
                     FAAKAGAKAVYAIEMNDIFYVGRELAERNNLTDRIKFIHGRSTEIDLPEKADLIIIED
                     FGPFFYYHNLLEVIRDARERFLKADGRFLPARITLYIAPFESQAWRDELDLFAADNDQ
                     LFGFDWSYTTEIAFNQTYYADSHPKKLLSAPFLLKTIDLKNETNFPFQKQFEINIKEN
                     GQIHGLIGWWDCWFTDQQFFSNSPQSGNNSWGQLIFPFRYPINVKSGESVKISLMVLE
                     SSPSGLIDYKWKITHHSGSQEQDTFSGRFFDLQQFNQMRRDATPSLNEKRLIQTFILN
                     QIDGKRSWDEIAEIVMKMFPEKFASKEDVFKIVLPLSNFLKGN"
     assembly_gap    57435..57534
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            57686..58624
                     /locus_tag="PLI55_00525"
     CDS             57686..58624
                     /locus_tag="PLI55_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018048926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPA99354.1"
                     /translation="MNLIIQIPCYNEEDTIEKTLNDLPRSIPGIDSIEILIIDDGCQD
                     RTVEIARQWGVNHFVHFNQNKGLARAFEAGLEKALELGADIIVNTDADNQYKGSDIAR
                     LVQPILAKKAEIVIGDRQIDKIEDFSWLKKKLEKFGSYVVRKVSQTNIPDVTSGFRAY
                     SREAALHINIISTFSYTLETIIEAGQKDIPIISVPIETNKTERPSRLFKSNWYYIRRS
                     FATIVRIYTMYQPLRVFFYTGITSFGIGLILSIRYLIFMIVGQGKGHIQSVIIAAVFL
                     IIGFFLLMIGLLADVIAANRKLQEETLYRIKKDSLK"
     gene            58691..59275
                     /locus_tag="PLI55_00530"
     CDS             58691..59275
                     /locus_tag="PLI55_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPA99355.1"
                     /translation="MKNQEAEGLLSPLIKKIRLKKAAKCIQNNSTVLDLACGAGYLRQ
                     FLPSQCKYYGIDRITPPSLNLFNAFLQLDILQPESFETIKEWLPEPKVDVVTMLAFIE
                     HIKNPQYLLKQSLLLLKPNGLLIITTPHPIGRKLHDSLAKLYLCSLSGAKEHENFFGQ
                     QDLKFLLKQSGYKLLSYERFLMGLNQLIIATPLK"
     gene            59307..60608
                     /locus_tag="PLI55_00535"
     CDS             59307..60608
                     /locus_tag="PLI55_00535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPA99356.1"
                     /translation="MKYFSRIQNLLDKYEIPIIFAILLIGLVIRLAVIITIEEPIDRD
                     ALEYYTIAKNLNEHHRFSIDGENPTIRRSPGYPFFLAFFMKIVGTNPNHLYTVQALIN
                     ILTIWIVYLALKQIGANATIRIIAILLFTFNPSFIYVNTFYAEILTMFIMSLLIYLTI
                     NQELFKNKNWNIILQGILIGVLVLIRPTFLYLPIFILVVSILIRIFFHNKKMKDALIT
                     AIIAILTLVPWTIRNRIIFNEWIPTESSSGLALWQANLEIENRTVWYSVTDIAKYEQQ
                     RTASAELQSKLRSEYLQKYDINSLVELNQFLEQKAIEIILRHPIRYCILCVNRFLIFW
                     FSPPIGSTTLKAISPVIFVLALLFRYLITILGICGLWQFARRDFSGSFILISLVVYLT
                     ALHSASHAIQRYFLPLIPLIYFALGYFLNNQKSLNRKGRQE"
     gene            60691..62187
                     /locus_tag="PLI55_00540"
     CDS             60691..62187
                     /locus_tag="PLI55_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HPA99357.1"
                     /translation="MADSVIEQAKSGVRWVGIGQVGYRLLSIASSIVLARLLMPDDFG
                     VIALARLVLGFVMLLSAWGIDASIIVEEKRPKQIANVAFWINFGIMLVLGLLVIGISP
                     WVKKFYDTPVLQPILIWMGLGLIFQSFELVPRTILNRDLNYKYLTIINLSIEIFTQLL
                     VILLAIFGCGVWSLVIPQILASPLRAIPYWIKTKWRPSLYIEKADIKQVLSFGKSILG
                     AELIRYLNTNTDYFLIGKLLSKDKLGYYSFAFNLANWPVENIVKVINTVSLPTLAKVQ
                     SDLAEMRRLFLRMTEMVSLVTFPIFGVLVGITYELLVVIFGTKWIPAVRPLQVIVIYG
                     IMRSLFSPAGRIFLIQKKTHYMFFINLAQFPFLVVAVWLGIRKAEIIGAAVGVTIVLS
                     IGGLITIIVVSKMLKMKITEFIRTISPGFLCCVIFLICGWFFKHLLFGLGLPMVLILL
                     FYLLFAGSLYALSLRLFYPRLFIDFVRTSISIIGITPRNVFEKLRGNQ"
BASE COUNT        17839 a        14207 c        11934 g        17856 t
ORIGIN      
        1 ttttcatgtc taatttttgt aagttttctg aaaaaaagcg atttgttgat gaaaaaactc
       61 tttctatact gccttaccgg ctttctttta tttcagtcgt gcggatttta tgggcgtcaa
      121 cgcggtccga ccgactggca tcgcattcct aaaccggact ataccattcc gccccaggct
      181 caatacattg ggggcttgaa aatttgcctc gatccggggc atggcggtga taatcatatt
      241 cagaattata agcgcggacc aaccgggttg cgcgaagccg agataaattt aagagtggct
      301 ttatatctca aagaattctt agaaaagtcc ggcgcaacgg taattttaac ccgtgatggt
      361 gactatgaaa ttgacttaaa aaaacgaact gagatggcga atttcaatca ggctgatatt
      421 tttatttctt tgcatcacaa tgccgcaacc aatcctcaaa ccaattatgc ttcgacctgg
      481 tatcacggcg acgccgattt tgcgccagcc agcctcgatt tagcacgtta tatccagcaa
      541 agcttagttg aaaatctgcg cctgcctcaa cagctaccga ccggtttgct ctcggattat
      601 ttgatgcacc cgaatggatt cggcgttctc cagaatctta atatgccggg cgttttactg
      661 gaaatttcct tcttcactaa tccccaagaa gaaaaattgc tcagcaaacc ggaatacaat
      721 cgcctcgaag cttatgctat ttacctcggt atcgtgcgct gggcggcggc tggcattccc
      781 aaagttgtcc taattgaacc acgccccgat acgacaattg ccgacaaaca gccgaccatt
      841 tacttgaaaa tcaaagacgg tctgcatgaa cgtcccggaa actggatgct cagtcgcgag
      901 caatatttca gtcggcaggt gaccttcttc ctcgacgatt cgattgcacc atttattctg
      961 gaccgcgatc aaaacctgat tattcatcta ccggaaaaac cactattcaa cggctggcat
     1021 actactcatg ccgaggtcgt caattacctt ggcaatcata atttgcccca tcgagaccgc
     1081 ttccggatcg caccgccggc tcagtttcta tttgcaaaag cctggaatga ccgtctgcct
     1141 gccgatggaa aagcttttac tggaattgat gtcttagctt tggacataga tagcctcgca
     1201 gtagccgatg aggatacggt aagtgccatc tccgactacg gaaaatggaa ccgccaagcc
     1261 gccgcaactt ctaacggatt agcaactttt tttcttcagg cagagtctca gccgggaacg
     1321 gcggttttgc aattttccgc cggtgaagcg catacatact tgacagtatc tttttgtgat
     1381 actaatacca ccctgattgc cggtttttgc cggagcgcaa aaaatagcct accgattgca
     1441 gatgttttag tcaccctttt gccagattcg gttcagcagt tgacaaacgc tgacgggatt
     1501 tattattttc cgcacgccaa acccggagat tacactttaa acttcctaaa aaatggctac
     1561 ttcagtcaga atttagaagt agtagttaat gacggaaaaa gtaacataga taccctaaaa
     1621 atgcgtccga ttttcaatgg tgtcctacat aatttcgttt tggtgctcga tccgcgcttt
     1681 ggggggcagg aatccggagt agaaataagt ccaaacttga attcggctca ggctaatttg
     1741 caattagctc aaatcctgaa aacactattt gaacaagccg gcgctgagat ttatctgact
     1801 cgcgatcgtg actccactct aagccgtgac cagcgtatta aactcagcaa ccaatttcca
     1861 ccgggcggtt attacttgag gttgaatctg gattgcaacc accaagctgg cagaatgttc
     1921 aagggctgcc attatgtcgg caacgaagag ggaaaacgcc ttttgcaatc aatggcaaat
     1981 cgtctgaccc gcgatggttt ctgtgaccaa attcaaatcg ccggttcagc tgaaccggaa
     2041 ttggtcagaa ccaatcggcg ggctttgaca ctcgatctgc atttaaaacc gcaggacagc
     2101 ctactaatca gcaacgaagc cagcctttcg cggctcgcct ggggaatttt tctggggttt
     2161 gtagaagaat taggtggaga aaatttaccg acgggagctt taacaataaa tatcagagcc
     2221 agtcataaac cactcgctga tgctgaaatc tttatcggtt cgtcactaag acaattaagc
     2281 aatcagaccg gtgcaatttc ctatgaatac cttgaaccag gtaaatatta cttatcaatc
     2341 agtcatccaa tttatggcaa acgtgaggaa atcttcatta tacaaaatag cagtgaattg
     2401 ttaatcaatt tgtcggaata gactgggata atatttactc tctaaaagga ttttcgcact
     2461 ttcgaaaggt tcccataata aaaaaacggc agtcatttct gactgccgtt tttcatagtt
     2521 tttacctgtt attcttagaa accgatagtg atagagaagt attgattggc atcaaaggtt
     2581 gccattggcc gaaaagcata atcaaacatc aggtttgtac cagccatctt caggtttata
     2641 ccgaatccga gagacatccc atattccgaa gtctcatcat actcccaatc gcttggataa
     2701 agtggaatta gataaccagc gcgagcaaac aacatatccc taaacgcgaa ctcagcccca
     2761 gccgacattt catcgtagga ataactgtag ttactataaa ctccgcataa attaatttta
     2821 cctaattcag ctaatttgta agaataagaa agaccgagat tcatacttgc cggaagttca
     2881 tgagacgcca tatccagttt ggttttacgg gaagtcgcat taggattagc aaatggaaca
     2941 cttgagtcaa attctattgc agtaccatcg aattcgatat tactgccaat attcttcatc
     3001 gtaactccga aagccagtcc tatatgagaa atatattgga gtcccaaatc gaaaccgact
     3061 gctgaggcac tcatcgtacc aaccttttcg ctgatcagtt ttacattagt accgaaaagg
     3121 attcgatcag tgaaacgttt agcgtaagtt gcactcaaag tcaaataatc gggacttaat
     3181 atttccccag taccttcagg catattaaca gttgtgacgg gaatgtctcc aatatttaga
     3241 atttgcagac taatcccaaa agcgccaatc tttcccgcat tggctccagc cgccaggtag
     3301 ctgaccttca tatcggcgaa gtaactcatt tgggtaaaag tcgcttcacc ggtttttaga
     3361 tctgccatac cggcgggatt ccagtacata gcatccgttc cagcaatgct tgcaatattc
     3421 gatccgctca aagcaacgtt cttggcaccc attggaataa gaagctccgg ggcaccggca
     3481 gtacccagtt taggagcact ggcgaagacg ctgtgagaac agacgatgag caatatcatt
     3541 agccctatta cgaaatattt gtttttcata ttatttctcc tttaaagtcc attattcatt
     3601 cacagcttaa taggtgtcca gtcgttcgtt aggagtaaag accgctaact taccgacttt
     3661 ttcgccaaca cctggtacct cgacatgata gatataaaca ctactcgcaa ccggaagccc
     3721 ataatggttt ttcaaatccc aatccataaa tggagtagtt gcatcgtttt tctccaattc
     3781 acgtaccaaa tgtcccgaca gatcgaatat cttaattgta catttctccg gaaggaaggt
     3841 aaaacgtacc cagcgttcaa agatattcat ttcaccgcta tgatagccgt aatagggatt
     3901 cgggaagacg ttgattttct ttaaatcttc cttcagatag gctgtttcaa cattagccgc
     3961 atccggagcc ttaaaagaaa atacatcatt tacggtatta atgttgctag catagatttg
     4021 aaattcgaaa gcatcctgta agaaaggacg tgtctcagac ctcgctcccc atccgccggc
     4081 atacattaca ttgttgtatg ttccatcaag agtaccattc aaataatccg tatattgttc
     4141 gtcataggta tcattcagta taaatacgta ttcacgtcca ccattgacac caaatttagc
     4201 atcatcaccc caacccatat cccagattag gtttgccgca ccatcatatt caggacgagg
     4261 gtcctcaaca aaggctactt ttaactgtcg aggtggatca ctttccatat cccaaaccgt
     4321 aaaaggaaca tcacctaagg ttgattgcac ttcataatta ggtctccaat aaacaacagc
     4381 cttttgccat ctgtctggat atgcggtttt actgacttcc attaacgccg cggcactcga
     4441 atccggctga ggccagcctg acggtccaat cccggcgaag cgaatctcaa catcaacata
     4501 gtctgccggt ccgagatcac ttccgaagaa ggtgaatccg tttccaaaag cgcctgcgaa
     4561 tgtctccatg ccccagttag tcggccagct aacccatctt tgacctccag ttatcccaaa
     4621 accgttgtaa cccggcatat caccaaacgg gccaggaata cttaagttaa tcccaggatc
     4681 gggaccaact actttcacca ttaaaccatc aaccacatta taagcatcat ttccagtctg
     4741 atttgcatta tttgccacca ccgtatcatt cggtgcttta atgaggttcc aggttaaatc
     4801 aggattaaag gtaacagaat aatcggctcc agttactcga gaaggatcta ccacccaggc
     4861 aattgtagaa ccatcactgg gggtagcatt gtttagagta gtatgggtta cttcgacagt
     4921 atcattataa gaagcgctat atctgactcc cgccgtaatt tggggagtaa ccttgacaat
     4981 ggtaggcgaa ctcgtaaaaa tctgaggcgc accaaattca agatagcctt ctgctacaac
     5041 accaaaataa tattcctttc cattaataag tgggtcattc acattgccga ctttgggatg
     5101 tttgtttact aatagccgga taaacctctg tttcgtgtat tccatcggat cggcattctt
     5161 gccaatatcc caggattcca cttttaccgg gtcagcacct gatgcatcag aatattgata
     5221 tacagtataa ccatagaaat tataagtcga atcggttatt actttctcag tattatcaat
     5281 aatgacagtg tcgccaaatg cttgcgctac tattggatcc ggtgaatcgt ataaacttcc
     5341 atcatcatta tattcagaat ttccactcca ggttaaaacg acttcattgg gaagttcact
     5401 gacagtcact tccggcgcag ctggtgcttt agccaaaaca aaattcaatt cataagccaa
     5461 ttgggctaaa ttatccactt cttttagagt tgttacacta ttcaaattag aagaaccgcg
     5521 cgcgacaatg acaccagcta cgatttcctg cacaccgggt tcaccgaatt cggccatgcc
     5581 atcaccatcg gagtcaaccc acggctccat cgtgaacggt ccggtcgtca taaagaaacg
     5641 cctatcagca ggattagaat ccaaccaacc aattccagtc tccggatcac cggaaaacat
     5701 gaactttgtt ggcggtttac ttggatctgt accaacccca ccttcggtaa tcggcgtacc
     5761 gtctctccag aaaccactca tataattata aacatccatt ccagaactgg gattaccctg
     5821 attggaatca tcattattat aatagatgaa agacgtcatc ttaagcattt tcttatcttg
     5881 taagacagtc ccatccggta aggcaactac actgccgact tctggtacta tcgggccttg
     5941 aaagaaatca ataccgacag ctggtggtgc ggcgccgtaa acttgatcgt tatcggtcgc
     6001 attgtaacag taaccaaggc ctaaagtcga atcacaacca acctgatcat caccagcatt
     6061 tcctaaatcg ggatcagacc agataacaaa gtaggtatct tcccagtttt taccactctt
     6121 attgactaaa gtccatttga taaagatcat atcacccatc gcatcagctc ggctgaaagc
     6181 ccaagccatt tgatgaactt cggcattcag tttatttgtg ccatattcag aatggggtgc
     6241 caggtcattc cagacgctat atatcgtctg gtcgccaatc aacatcggct ttccatcttt
     6301 atctaatggt gccccctgat cggtaggcca gttagtccaa tcaccgacgc cattaggcct
     6361 gatttcatac cacttatatt tgggatcggc tttattgtca gcaacatcgg ttgctaaaat
     6421 caaaccgggt tgaaattctg ttgcagagtg ttgcacacca gcgacacgaa tttcaccatc
     6481 aattttagca ccgatatatt gtccagcacc aaagattgga cttagcccac tacctttcgg
     6541 ccattccaat ccccaatctc ctaatacgtt atcataaaac caagtcccat tatttcgaaa
     6601 aacgccattc attcgattgg cattcaccca gatatcactg gtcatggctg ctttttccaa
     6661 cgcccgggtt ttataaatat cctctgctcc aattacgagc gtcgaaaccg ttaatattat
     6721 cataaatatt gacaatattt ttttcatata ctttcctcca ttataatgag ttatcttcat
     6781 agttcgctcg ttatagttca agcatgagtc ccaggcgaat ctgacgcggc gagccataat
     6841 ggttataatc catctcgcgc atcttatata gactcttctg ctcatcagtt aaactattcc
     6901 aatattgccg gccagccgat gtccctaaat atccagtatc atccggtaaa ccggtagtaa
     6961 tccagacatt tgcgacgttc tcggtattca gtagattcaa agcccaggca taagctgtta
     7021 attgaacacc accaatgtaa aactttttat ccagtttcaa atcaactcta aaaacccagg
     7081 gtgtcgtctg agaattaact gctgataccg gcgtctcgga aacgttggta ttgtcatagc
     7141 gaccggtaaa aggcatagta ttgaaaacct gcatcagcgt atagggatgc cccgaattga
     7201 aactaaacat caagtttgcc ccggcattat gtaaaaactt aggaccttcg gttctaccga
     7261 gacgataatc taaatttaaa ctaccagtat gtctttgctc gaaatccagc ggcattgcaa
     7321 atttcgggaa atttccacga gcgccaccct gccaagcgat attaaagttg gaatttgtcg
     7381 ctgaaccagt accggtcgca tactgtagct cataattgaa tgtcgccgct aaattcttat
     7441 agcgtctgag attaaaaata atatccaagc ctttgacagt tccgaaatct ccattccgac
     7501 tgaaggcaat attggtcaca tccgtagtaa cgttcattac ctggattaag tcttcagttt
     7561 ctttatagaa agtggtcaac tgcacacttg cgaggttacc cagcatctgt ttaaaaccaa
     7621 tttcataaga agtagttttt ggcggtttaa gatttgggtt atccaatgtc gtaaaatatc
     7681 cgccccgact tacaaaggct tccaaataaa atgggctcaa gtataaatct tgcaggctcg
     7741 gagcttggta aaattgccca tactgcgcgt gaaaaacagt ttgatcggtt acaggaaaac
     7801 tgattcccag acggggactg atataggtat gtacttcaga atcttcgaca tctgtcttat
     7861 caaatttttc atttccacca aacattccag tacccggtat ataactgccg tcagccgccc
     7921 attcggcatc cagttgtttg aactgccagg cattcggatt aatatgatca tatctgagac
     7981 cgatatttag cactaaatct ctgaattcaa tcttatcctg aatgtagccg ctcagaataa
     8041 tcgggtgata aggagcctgt ttctggtaac catcggctaa tcttccagcg tcatcataaa
     8101 cggcatcatc aagaaaatca ccttcttcaa tctctttacc atcaatatca tagccataaa
     8161 aacggacatc ggcagactga tatttttcaa gatcagatag gtttgtcttt gctagataaa
     8221 ccggtgccag tacccgatat tctctcaacg tgtgatattt atattcgaag ccggctttaa
     8281 aagtatgatt tttacccatc tggtgaacaa tgtcaaaatc aatgccccaa taagttgtcc
     8341 ggttcttgaa ataatcgtca tactgacaac ctggctgaaa ataatccggt gtaatgcgac
     8401 cggtataagc aaccccgaca taggcattgt catccggcca gggattcaac cacggatcac
     8461 catattttac tagatcatca ccgaaggttg cgtcataaat cttccgctta gtatcaaacc
     8521 aattgaattt caggtcgtaa taggtgttct gtctgatcgt atgggttagg gtagcattga
     8581 atgaatgcga ataacgactt tcttctccca tatgctcagt attgaaaata tcaatcggag
     8641 tgacggaact aatgatgcga tcggtagaaa catagctggt tttcaatacc atatttttac
     8701 ccagctggaa cttgagcttg gcgttccatg accaatcaga cgttgaattg cctggtatgc
     8761 gtgctgttag atcataatta tgggtaatag tatctaagaa agtattacct tgatcatcaa
     8821 tcttagttaa aacttgatca tacgtaaaat catccaaacg ataatcattt tccgcccacc
     8881 cgtaagatgg ccaggcatca gccgcatacc gccgtgtccc gctgataaaa aatgtgtgat
     8941 tacttttcgg tataattggt ccgccaatag aagctacata ttcgttaaat ccataaccgt
     9001 aagtacccaa gattttctca tcagccttta aaaattcatc cgtaattacc tggaatgatg
     9061 cagaataatt ctcactgccg gcgtttgttg taattgcaac gataccggac atagcatcac
     9121 catattctgc attaaaaccg ccggtctgaa cactcatctc cgcgatggct tcgttcggca
     9181 aataagtgta aacagcatta ttgtatggat catttactaa aacaccatca atataaagag
     9241 ctgtttcagc actacgtcca ccccggatat tcatagcggt gctgttttcc gctttgacaa
     9301 caccagcagt actagcagca atttcttgca tacctcgtaa aggcatattg gcaatttctt
     9361 ctgctgactt aataatgttg gtgttagtag catcttttct gatcattggc ctttcggcgg
     9421 tcaccaccac ttgttcaaat tccaacgtag ttcgctccat ctcaaaattt aactctgtgg
     9481 ttaaatcgag tgacacagcg acattgttgt aaactatctc tttatagcca atgtagcttg
     9541 atcgcacggt ataagtacca acaggaacat tgagaatgct gtaatagcca tccagatcgg
     9601 tggctgcccc aagattatat ccaccaccgg taacgataat atttacaccg gctagaggag
     9661 cacctgtgtt cttgtcaaca actgaacctg cgattttacc agaggttccc gcgaagagaa
     9721 caataggtag caacattgct aaaagacaaa tcgctacacg acgtgccata caaatcctcc
     9781 ttttttaata ctattgttgt ttgttcaatt acattacctc actgcatggt aaatgtaaag
     9841 aatttatttt tctaatgtca agctttttta ttctattttt gtataaaaat aaattatttt
     9901 tttatccgcg cgggtaaaat ttcatcaatt atcccatatt tcagggcttc ttcgctcgac
     9961 ataaaatagt tacggtcagt gtctcggttt attttttcaa gtggttgacc ggtcaattcc
    10021 gccaagattc gattcgtatt ttcctttaaa cgaattattt cacgagcgtg aatctcgata
    10081 tcgctggctt ggccttccac gccgcctaat ggttgatgaa tcataatgcg cgaattgggc
    10141 aaagcctggc gtttgcctct ggtaccgctg gcgagtagaa ccgccgccat gctggcggcc
    10201 aaaccgagac aaatcgttac catatccggc ttaatgtaat tcatcgtatc cagaatcgcc
    10261 attccagccg ttatggagcc acccggacta tttatatata agtaaatgtc tttcttggca
    10321 tcctcagccg ccagaaaaag caattgggca atgaccaaat tagccacgtg atcgtctatc
    10381 ggcagtccaa gaaatataat gcgttccttg agcaatcgcg agtaaatatc atacgaacgt
    10441 tccccccgct cggtctgctc aatgacaaat ggaacataag cttgagcctt agttttcatc
    10501 gttacctcct tttattgtaa ggattcggta tgaacggtta cttttttctc tttgataaat
    10561 tgatggatat gctcccgaat cttgcgatct tccaggttta acctaatctc cagccgactg
    10621 cgggatttct gatagaattg ttccgcaaac tctttctgct cttccggcac aatttgcagg
    10681 attttatcta tctccgtttg gatttcgtct tcgcttaccc taatatcttc ttgttttcga
    10741 acagcctgtt gaataagata ccaccgaata tttttttcgg caattggtaa atattcttcc
    10801 ctgattttgg cttcgtcaat gggtttattt tgggaatttt ctttcatttt ttcaattaaa
    10861 ctatccaaat aggaattgat tcggtaaggc ggaatatcaa attttgtatt ttcaataaaa
    10921 taatcggcaa gggcttggtc taattcttcc tgagcccgct gattccattt atattgcagg
    10981 ttttccgtaa ttcgctgacg aaattccgcc acggtttgaa catcctccac ctgactttta
    11041 actaattcat cagtaagttc gggtaaggta tgtttttcaa ctcgttttac atctaccaag
    11101 tattttagca ttttaccaga cgacgatttc acgttcaagt tgacactatc accggctttg
    11161 acccccttaa aattaccggc ttccttatcg ccgaaaatcc cttcgccgac tttaatcagc
    11221 cgatccggta agcgtttgcc aattatcgga atgccgcctt catccagagc ctgcaaatct
    11281 gccacgagaa aatcaccatc ttgagcaccg tcaaaaacct cctgcacctc cgcctgattt
    11341 tctaggaaat cttcgatgta acgctcaaca gtttccggat caacaatata ttctttctgc
    11401 tgaattttta tgcccttttt ataatcaggc aaagtaattt ccggatcata ttcgacttta
    11461 atagtgtaat taaaaggtgt attttcatca aaatccaagt cagtaatgtg caaatcgaga
    11521 tagtcatcta ttcccacatc tttcaaggct tgcttatgag ttttttcgat aatctgatta
    11581 gtaaaactca cgaaaatttg aggcttaagc aggcgtttag ccaaactttc cggcactttg
    11641 cccttgcgaa acccatcaat tttcaaatcg cggcgtaaat cgttaaatac tgcgatataa
    11701 tcgtcggcaa tttcgtccca gtcaaccacc actttaactg ttttctcgat cggtgtacta
    11761 ttaattaatt ccgctttcat tgaaattcct atctatttat tttttcaatt tgagaagtgc
    11821 cgcgggcgag atttgaactc gcacccctat taaggactgg atcctaagtc cagcgcgtct
    11881 gccaattccg ccaccgcggc tcaaaaaacg cccaaattta accgccactc tgcgattttc
    11941 caaagtgatt aacaatgata atcgtgtaaa aatagtgact ttagataatt tgacctaatc
    12001 aacttgcgtc tatcatatag gatagaatgt aagttcccag aaaaaatcca ccggtcagtt
    12061 accagcacca tggcaatatt tgtatttttt cccactaccg caggggcagg gatcattgcg
    12121 gccaactttg gcttcaaccc gtaccggtcg aattttagcc tgctgtccat cactggtggc
    12181 gtattgcggt tgttcttccg tctccgattg cattcccggg atgaaaccca taccgtcggc
    12241 ttgttggtgc atcaagcgca tcgtagaagg gcggcgaacc tggcgagttt caggttgcgg
    12301 gccgccaatt tgggctcgga aacagagtcg caaggtttgt tgattaatgt catttaccat
    12361 ctgaacaaaa gcgtcgaacg cttcaccttt atattccagc agaggatcct tttggccata
    12421 agcgcgccag tttatcccct ctttgagttg atccatagca tataagtgat cacgccattt
    12481 ttcgtcaata gtacgcaaca ttataaaacg ttccagaaag cgcatctgct cagcgccgta
    12541 aattttttcc ttccgttcgt aatattgctt agcgcggtca ataatggctt gtttaagcgc
    12601 ttcaggtgta tattcggata attcactggg atctaaatca attccaagac tggtagtaag
    12661 gtccatccgc aatgccatcc attgccaatc accagaatct ttaacctgag cgttatattt
    12721 ttggaccagg tcgtctgccc agtcttcaag tatgttatac agttcgcctt tagtatcact
    12781 acctttcagg gcataattgc gacgggcgta gataatttca cgctgatgat tcattacatc
    12841 gtcatattcc agcaagttct tgcggatagt gaaattgcgc tcctcgactc gcctttgtgc
    12901 tcgttcgatc gaacgggtta ccatcggatg agtaatgacc tgaccttctt cgacaccgag
    12961 ccgatccata attgccgcaa tgcgctcact attaaaaagt cgcattaaat catcttcgag
    13021 cgaaaggaaa aagcgtgatg ctcctgggtc gccctgacgt ccgctacgac cgcgcaattg
    13081 caaatcaatg cggcgggatt catggcgttc cgtaccgatg atatagagcc ctccaagttc
    13141 aacaacgcct tgccctaact taatatcggt gccacgtcca gccatattgg tagcaattgt
    13201 cacggcaccc cgttctccgg ctcgcgccac aatttctgcc tcacgtttat gattcttagc
    13261 attcaatacg ttgtgaggaa tgccacgtcg tttcaacata cggctaatcg tttcagaaac
    13321 ctcaacgctg atagtaccga ccagcaccgg ctggccacgg cggaaggaag tttcaatttc
    13381 attaataatc gcattgtatt tttcacgacg agttttatag acttgatctt cgtaatctat
    13441 gcggatgcag ggcttatggg ttgggataac cacaacttct aatttataaa tatcggcaaa
    13501 ttcagctgat tcggtagcgg ctgtaccggt cattcccgct aatttttcat acattcggaa
    13561 gtaattttgc agggtaatcg tcgcaaaagt ttgggtttcg gcttcgacct tgaccttttc
    13621 cttggcttcg atagcctgat gcaatccatc actatagcgc cgccccggca gaatccggcc
    13681 ggtgaattcg tccacgatca ggactttacc gtcctggata acatactcga catccttttc
    13741 aaataaggta taagctctta gtaattgcga aatattgtga taccgatcgg agatttccga
    13801 atgctgggta tcgagctcca ttttgcgctt ttctttctct acctcatcta gttccggatt
    13861 ctcgtcaatt ttgacgaatt cctcagccag atcaggaatt atgagttcat cgagcttgat
    13921 tcctaacaat gtagccagtt tttcttcacc ggtaggagtt aaagttatgt tattattttt
    13981 ttcgtcaatg aagtaatata aatccttgaa aaattcatcc tgactgattg aagtcccggt
    14041 tttggcattc gtgagtaaca aatattcctt ctcaccactt tgtaacattt tttgataacc
    14101 cggttcttcc agtaatttgc gcaagcggcg attcttaggc atcccttgag aagcgataaa
    14161 taactggcgt gcaaattcag aataatcttt ctccggaatg gattctggcg aatctggaaa
    14221 acgactcagg atttccgtta ttttatcgcg ttgataacgc acaattgtac cgacggctgg
    14281 gttgagttcg tcaaattttt catgccttga actttgaacc ggtcccgaaa ttatcagtgg
    14341 tgtgcgcgct tcatcaatta aaatattgtc aacttcgtca acaatagcat aataatagct
    14401 acgttgaact tgctcttcag ccgaaagagc catattatca cgcaagtaat caaaaccaaa
    14461 ttcattatta gttccgtaag taatatcgca agcgtacatt ttcttacgct cttcggaatc
    14521 catcgaatta agaatacagc cgaccgtcag accaagctcg gtatagattc gtcccatcca
    14581 ctcactatca cgctgggcta aataatcatt gaccgtaacg atatggactc cgcgaccagt
    14641 cagggcattc aaaaaaaccg gcattgtcgc caccagagtt ttaccttcgc cggttttcat
    14701 ttcggctatt ttaccctgat gtaaaacaat cgccccaatc aattgaacat cataaggaat
    14761 catatcccag actatcttct ggtcagtgac taaccattct tgccccacca gccgtcggca
    14821 aacttctttt accatggcaa aggcttccgg caaaattgca tttagagcgt tttgctcggc
    14881 ggtttggatg agtttttggg cttcctcttc tgagattcca gattcttcgg cttcgattcg
    14941 ggctgcctca cgagcagttt gaatttgaac tttaagctca gctgtgcgtt cgcgcaattt
    15001 ttctattggt tgctcagaca gggttgcata aatacgatta atttctgcta cgagcgggag
    15061 aatttttttg atctcacggt cagaagaggt tccaaaaatt ttcgttaagt aagtagctat
    15121 tcccatttta tcttaccaaa ttatgtttat tgtatttgac taaaacactc aaaatttgtt
    15181 tccggaaatt tgtcgagttt tccgaaacta ttcttctcga gtagccgact tggtttccga
    15241 gcgcttcaat ttataagtta aagtatcgct aatatcgtgc aggaccgcat ccaaaatccc
    15301 attaacaaag gcactactat cgtcggtaga atatattttg gcaatttcaa tagcttccga
    15361 aatagagact ttcggtggaa tatcattgaa atatttaaat tcactaatcg ccattcgaat
    15421 taccagacgg tcaatgatgg caatccgttc aaaatcccaa ttttctgatt tttctctaat
    15481 gatttcatcc agttcgtcac ggtgggtaac cgccgctatg aataaatcct tggcaaaagt
    15541 ttttaactca tcctgaagtt cagaattttc aatcacatca tcaattatct gagctagagg
    15601 gtagctggtt aactcttccg catataaagc ttgcagagcc acttcgcgcg cggctcgtct
    15661 ttctctcatc gttacaactg atccttaacc ataacaaata acaacgccaa aatttacagt
    15721 atttttgggg caatatacaa tttttctcac aactgaattt tcagagcctg cgaaacacgt
    15781 gcaatgaatc aaaaatattt ttgagatttt tgccctctca tctatccatt cgcacaatcg
    15841 tacctcaaga caaactcata ataaatattt tgttagtaga acttaaacgg acttaaaaac
    15901 gcttataagc acttaagaga actttcagac aaccgcgcaa cctttacaac tacaacacga
    15961 acaattggca aacagcaaaa aataaggtaa atttcaaatt tgaaatgact atcaatgaat
    16021 aagaaaaaat ttccgcaggc aaactaaaat gccacctaaa aaaattgaca acacccaaat
    16081 cccaacctcc aatgtggaaa agtactatct cagcctctct ggcaggcaat tcatctatcc
    16141 gccgacagag aaaatcccag tcattcaggt agcgaatttt ccgatgctcg gtcaattgac
    16201 cgccctccgg tttttagaat gggtacaaaa taatcccgat ggggtaattg ctctgccaac
    16261 cggtaaaact cccgagcatt ttatcaagtg ggtggggtat tatctaacca attggcactc
    16321 ggctgaggtc attaaggatc tcgaaatggt ggaaattgat ccggcacgcc gaccggatat
    16381 ggcgcgcctg cattttgttc agattgatga attttatccg attgatccca atcagcagaa
    16441 cagcttttat tattacattc aaaaatacta catcaagaat ttcggacttg acccagaaaa
    16501 ggcacttttg attaatctct atgaaattgg cacagctaat aatctgagta ttgccgaaat
    16561 tttcggcgat gagaaagccg acttgagttt gcggacacgc tgggcgaaaa atcccatcga
    16621 agaattgcaa aaggaaacca tcgcaagaac cgaccaattc tgcgtcaact acgagagcaa
    16681 aatccgggca ttaggtggaa ttggcttctt tctgggcggt attggaccgg atggccatat
    16741 cgctttcaat attcgaggca gtgatcatta ttcaacaact cgtctaatgg aaaccaacta
    16801 cgaaacccaa gccgccgcgg ctagcgattt aggcggcatc gaaatagccc gcaaacgtct
    16861 ggtgattacg attggcctcg atactattac ctataataaa gatgccaccg caattatcat
    16921 cgccgccggt caagctaaag ccaatattgt gcgcgattct atccaaaatt ttccttcaat
    16981 ccagtatcca gccaccgttt tgcaaaaatt acctaacgcc cgcttctact tgacggaagg
    17041 cgcagccagc aaactgttcg agagacgata tgaaaaaatg gcaaagtcgc ccgaattgga
    17101 gccgaaaacc atcgagaaag cagtcattaa tctcactctc gaaaaaaata aaaaacttac
    17161 cgacttaacc ctcgcggatt ttaactctaa ccgatttacc gaattgattc ttaagcgtac
    17221 gaatcagacc cataccgaaa tctgtagaaa tttacacgag tctattatca accggataga
    17281 tcgtggcatt tattatttag acaacgaagt ctttatgcac acttcaccac atcacgacga
    17341 tgaaatgctc ggttacctcc cttatattgt tcatctcgtg agaaatccac gtaacaaaca
    17401 ttatttcacc tatatgacca gtggattcac cgccgtgact aattcctatg tactggaacg
    17461 attgaaaaat ctacaagatt tggtagatag ccctactttc caggttttaa tgcaggaaca
    17521 ctatttcgac cccgccaaca ccgccgcccg taaccgggac gtcaatttgt atctcgacgg
    17581 ggttgccctt tatgatgtta agaaaaaaac tgaagccgaa tcgcgccgac tattatccat
    17641 tttgactgaa atctatgaag aaaataacgt catctacctc aaagaccgca ttagcgaatt
    17701 aatcaattat tgcgcaaccc aataccccgg taaaaaagac atcgcgcaca ttcagaaatt
    17761 aaagggaatg attcgagaat gggaagctga tgttctctgg ggctattttg gttttaatag
    17821 caataatgtc tttcaccttc gtttaggttt ttataaaggc gatatttttt cggaagaacc
    17881 gacgttggat cgcgatgtcc ttcctattta ccaattaatg aaaaaaataa gaccgaccgt
    17941 cattacggta gcccttgacc cagaaggcag tggtcctgac acgcattaca aagttctgca
    18001 agccatcact gaggcactca aactttatga aaaagaagaa gatatctcta atctacgcat
    18061 ctggggctat cgcaacgtct ggcaccgttt tcatcctgcc agcaccaata tttacgttcc
    18121 ggtcagcctg aactcaatgg ctattatgga gaatgccttt atggaaagct tcggctctca
    18181 aagatcggct agctttccca gctgggagtt cgatggaccg ttctgccggt tggcgcgcaa
    18241 gattcaggtt gatcaatact taaaaatcaa gacttgtctg ggacgcgatt tttttatcaa
    18301 aaatgaacat cctcgattgc gcgctaccca cggatttgtt ttcatcaaag aattgacacc
    18361 aaaggaattt tatcgtcatt ctgaggagtc gcgccgggtc accgaaaatc tataatatcg
    18421 aatgaaaaca acttgttatt tatcaatccg gcaagcatca ataaattcca gaagtaggcg
    18481 gactatcatt ttatttggaa ttgataagca atatattttt gactggcata ttgatctcca
    18541 tataatcaga agatattaat gttaattgtt ctgtgtttta ttcgaaaatt tcatcggttt
    18601 caacattttc aatatgctat taataatggt agtagctgga ttaatagctc cgatcaacgg
    18661 cttgggaata aagtcatttg aattgctttt tatggctatc ctctcgttag caatttatcc
    18721 aattactctt aaaggcttaa aaatcaaccg attcgaggat ctttttcttt tgttactgta
    18781 tggtggttat ctttataaaa attagcctta aatcgcagta tttatagtaa tgcatcgttt
    18841 tcagctattt tcgctaatca ttttttgggc tagtatttca aactgctcca atccaatttc
    18901 tccaaagcca gaaaatccgt tcaaaaatat taatctgcaa atattatcgg acagcaccct
    18961 ggcaataacc tggcaaggag agttcgaatc agaagcactg ctcagcctcg atcgcaaagt
    19021 cggtgaagca tcttggcaaa tggattatac caatgtgcca gctaatgatt ctatctatgt
    19081 agatacgcta tattccgttc acactgatac ggtctattcc taccgtttac gtttcaaaga
    19141 aagtgataat tcatcttcaa tttctccaag cgtagcgctt ttatcggagc gatgtgtacc
    19201 ggtagatttt tcagcgcggc aaatagataa atccacaatc cggattgctt ggcgagacca
    19261 atccttcggt gaagccggtt tccgaatcga tcgccaggtt ggtaatgacg attggcaaat
    19321 cgcttatgcc aaagccgatt ccaattccta tgaatatttc gacataacca ctaccagttc
    19381 agagattagc taccggatcg ccgcttatgc cggatcatca ttttcttctt tcacagaaat
    19441 agcaaaaatt aaattaggtt cactcacttt agctaacctt tattttggta ctgaaccgac
    19501 ctttgaggta atgacctgga atatcgagga attccctcgt tttgacaccc ttaccgtaag
    19561 cgctgtcagt caagccatta aagccctcga cattgacatt atcggcatgc aagaaataaa
    19621 aagtgggacg gcttttcaac aattactgga ttcgctcgat acttgggacg gataccgcac
    19681 caatagcgcg gcttacggta ttaatctagc tttcgtgtat aatcctgcaa atatcaatgt
    19741 caatcaatat tatgaaatcc ttgaacatga ggacgctttt ccccgcccgc cgctggtgat
    19801 agaattggaa tggcaaaacg ttcctattat tgtcattaat aatcatttta aagcaatggg
    19861 cgatgaagat tcacaaaatc atcgtctgca agcctgcact ttattagaag aatatattag
    19921 aacaaatctc gctgacgata atgtcatcgt aatcggcgat ttaaatgatg aattgaccga
    19981 tgctcagtca gttaatgttt ttgggccatt tttaagccaa ccttccgcct acaaatttgc
    20041 cgattactct attgctcaag gtagcagtaa atactggtca tatccaacct ggccaagtca
    20101 tattgaccac attctcatca ccgatgaatt attcggcgtt ttcgccaatt ctgcatccgt
    20161 gattaaaaca ttattgattg aaaattatat ggaaggtggc tggaatgaat atgcggttaa
    20221 aatttccgac caccgcccag tggcgctcaa gctcgctttc taaggctttt ctcctctacc
    20281 tactaaagca cttgcaattc ccaatattgg ttataaatta aatcgtaaga tttttaaaag
    20341 cgggaggtat gatgttaaat atcataaaaa gcttcgtcgc tattctgaaa atcgaaatcg
    20401 aagacttagc cgccgatgtt gaattgatga tgaatgaatg ccagaagaaa aaagatgctg
    20461 gcaaaatcac cgattatgtt tacctggaaa atatggcgct ctatcggcat gaattgctgg
    20521 ctgtccgttc gtttgcccga ctgctggaat ccactgaact gagtaatttc gaaactcttg
    20581 atgccctaat cacttattta caagagtctt tccgtgaagc cgggtatgtg cgagcaatca
    20641 atatctgcgt cgaacgcaaa atgaaaaaag tcgccggtta cgtatcccgg taatcaaagt
    20701 ttccccggtt tgatagacct gcggcttgtc tgctatcatt caattaccaa taatttaata
    20761 atctaaatcg gtgcttgcga ctcaaagcct taggtttagt tcctttccat ttcatctgtc
    20821 atcgtttcgt ttcaagacta ataatcgact tttcaggtgc tcgatccccc agcctgaaaa
    20881 taaaaatttg ataaacatct gcgccggtga taatttttag cggagtttga cttgaagtta
    20941 ctcgatcgct atatactcaa gaaatttcta ctcattttcg ggctttgcct acttgctttt
    21001 ctggttattt tcaatatcgt tgatttaatc gaaaaactag ataaatttct caaagcggat
    21061 atgccgtcca atttgatagc tcttttttac ttgtaccaat taccttatta ccttaatctt
    21121 gcccttccaa tggctttact gttagcggcg gtattcacca tcgggctgtt atcgaaacat
    21181 aatgagctag acgccatcaa atcctccggt atcagtcttt atcgggtttc gattccttta
    21241 ctaatcgccg gaattttaat tagcatcggc tcgtttttct ttgaagatgc cgtagtaatc
    21301 cctgctaccc gtcggcgaat tgatattgag gcacataata tgcgccgtcg taaatatata
    21361 cctaaaactt tctttaccaa cattatgtat caggattcac caaactgcaa catcgtgatt
    21421 ggcaattttt cgacgaaaga taatatcgcc cagaatgtca ccattcaata taacgatcag
    21481 ttcaggctgg tgcgacgcat tgatgctcgc aaaatggtct ggttgcctga acaacaactc
    21541 tggcaattat tcgactataa agttcgctcc ttcgaccaag acggtactga aatcgtggct
    21601 gccgcctcca gcgatacctt gattcagctt aatttacaac cggaagatat tgtcaagagc
    21661 gaccttaatc cggaaggtat gcgttaccgc gaattagcct attttattaa gcgtcttcaa
    21721 gaaagcggta atgacgctcg taagtgggaa gtcaataagc atttcaagtt agcttttcct
    21781 ttcaccaatt ttattgtaat tcttttcggg gttcccctgg cggcggctaa accgcataag
    21841 gggattgcct tcggcgctgg tatgagttta ttggcgatat tcatctatta tggatttatc
    21901 aagttaggtc aggtattggg ttacaaagga atccttagtc cgcttctcgc ggtctggctc
    21961 ggtaatatag tcttcctgat cgccggaggt tacttacttt ataaaatccg acagtagcgg
    22021 aaaaacttta atttttcctc aaccgcataa tttgccggat aataatagat aattttacga
    22081 gtggatgagt ttttaagcac ttttaagtac attgaagaaa atttttaacc tcaggctttt
    22141 tatgtttaat tgcccaaggg tacgattgaa tgactgaacg agtttgattt tacaataaga
    22201 tagcaattaa ttgacaattg agtaattatt atagttctcg ataattgcgc taactatcta
    22261 taggaagtgt ttgccctttt ggtcgaataa catagcatat tgaaattaag accggtggtt
    22321 gtcgtcggcc ataatccatg attaaaaccc agcgcccaat ccaggtgaat caatccccag
    22381 tggaaaccac ttccgaaggt caattcccgt gaactgtgcc cgccaaaaga cagccctact
    22441 cttagcggga agcgctgtga tttagttgac tctaaaccca gacaccactt ccaggcgcca
    22501 aacgaataca aacgatcctc aaaaccaact actaaatccg aagccagcgt tatattttct
    22561 tccaatattt tggccgcact gaatcgtacc aaactgggat atttcacccg aaatgcggtt
    22621 gtatctttaa ttttatatcc ttcaccatga aagacatcgt ccacactagc ttttccaaaa
    22681 aacttatcga accgggcttc atcaatacta aacacatatt tgtaaaaatt accctcccac
    22741 ggtaaaactt tttccactga aaccgatcga gacaatagag tgcttttatt ccaggttata
    22801 gatccaaaca gattagtcag gcttaagccg agtcggtaac catttaattc ttcggtcgtc
    22861 agtccaatat ccagcgcaaa accgcgccca cctaacgatt gctgaaaata atactgtcca
    22921 taaccgtcaa tataagtccg ctcaggctca aaataagtag taaccgagcc ataggaagaa
    22981 tccggattta acccgatata ggcaagacct tgcagatatt tagccgtaaa tcccagaaaa
    23041 atgttaccga ctggaaaagc cagtgaatag ccccactgaa ttacagccat tacctcctcg
    23101 ttgaaatcca ggtcgagagt ttctccgatt gggttttcgt caaagacaaa attcagtaaa
    23161 tccttcggca agcctaagtc tccaatcacg atcaagtcag agctgatcgc gcgattatac
    23221 gtcgaataac tgatcaacgg tagaggaaaa tatatgtcgg taaaaacccg ccagccttca
    23281 tcgggaatgt cgttcataaa ttccttcttt aaatggccat tgcctgcttc tagatcctta
    23341 ccgttatatt tttgatactg tttaattgaa aggaagttag ttgacacatt ggttccaaat
    23401 ccaccgaaaa tacgatatga atggtattca tggggaatag ccagattagc cggattataa
    23461 tctatggcaa aaataccgcg ggctaacgag ccataagctc cggctagtgc taaagcgcgt
    23521 ggatcgcgtc gcgtctgagc cgttgccata atgctcatcc cgagcatcag taacagagtg
    23581 attttatacc tattgtttaa ccagcccatt tttttcttcg ccaaccaacc agctgtgaaa
    23641 tcgtaaatgt actacggaac agttgcaggc gtttcatttt cattattgtt attaatcagt
    23701 tccatcgtct caactgtcag cgatatcgcc gccagaattc ttacctcatc ataataacca
    23761 acaatcacaa aatcttcggt tgcattaaaa tgcactcgcg gccgaatgta atgagtttcg
    23821 tctcgtaaaa ttgcttccat tttagccgaa tcaaggggcg tttcggcaag ggtaaagccg
    23881 ggagttttta cacttccatt actgttaaaa gcctccggcg gcggcaattc gactaacagc
    23941 aaggtatcaa ttattatcgg aacatcgccg gtgtcagttc tggcgtacaa ggtatcgttt
    24001 aaccagaaat agcctgcgtc cagattgtcc ggttcgagag gaaaataatc ataattcgac
    24061 attaatatgg ataaattacc ggaaaatgga aaatcattga ttatctcagt ataaatggaa
    24121 gattttatca gaccatcgcg gattttctgg cgggtctcgg gatcaatagc cgccatctct
    24181 gttgaattga caggaataaa agtcacttgc ccgatcttta cctgaaatgg aacggtaatc
    24241 tcataaaagc cccaaaaggc tttgccgatt tgcaggcggg aagtatcgtc ggaggggatt
    24301 atggcttggc cgctgaccaa aatcgaatcc ggtaccatat taaaaatcgg cgcaatatca
    24361 atctccattg ttgattcttg aattggatta gctggtgatg ctttggcaat cgaaccgtct
    24421 tccgtaatcg tctggggtgc gacgcctttc ttcaaccctc tcagcactaa attgacattg
    24481 gtacgagctt gtatttcatt gaagaaagtc aacttcaaaa aaacttggcc gaaatcgaca
    24541 ttcgctaacc ccgtcggaat attggctatc gccattggtg gtatgtcaaa tgattcattg
    24601 aaaaatcctt ccagcatatc gaatttgagg cgcgcaaaat ataggttaac atcaatagag
    24661 gcatcaccca tatcggtcgg gattgtaacc acctgttcac ctgcattcgg taaaattgtt
    24721 gcaattattt cgaccaaaaa cgaatccact accgtagtag gatcaggatt acgaaaaata
    24781 taaccatcca ggcgtgcggg attctcgaca gttagtccag actgaacaat cgtgtcaata
    24841 gtgagaggtt cattcctgat ataaaaatta tgaaaaatca cctgcatcct gagattgaat
    24901 ggcatcaggt tatgaattgt caaatcaaga aaattggtat cgagcggaga ggtggttgtc
    24961 tgcgcaattg tcgccttgaa aattgtgtta ttagatggtg gcaataactg attttgagta
    25021 gttacaatcg gcttgggaat tccggtaaag ctttcaattt cttcaatatt cgtgtcaaat
    25081 ttgattgcaa taaaaggatc ggtattagca ggaattgtca ccggctgtcc ctgactgcca
    25141 gccattaccg ccgtaacaat tacttcgatt gaatcaaata attgggcgcc gtttaaatta
    25201 cctggagtac cgtcgcttag cgtcgcaccg gctgggacat tttcataaaa agattcggca
    25261 attagtgaac cgtttttatt acgcaaggta attcggaccg aatccatatc taccggcaat
    25321 tggttatcaa tttccgtttg gaaggtgccg gagcctaccg tcagataatc tacccgctca
    25381 aaataaggaa catcatcctt gtcaaaaacc cgatatttat agcgctggcc atccaattca
    25441 tagcgcgcaa tctcaccgat gagcaactca ttcggcgaag gtaaagtacc gggaatggtg
    25501 attatttccg atagtttggt agtttgggta accgatctac taaaagtcaa ctgagattcg
    25561 ctgagttccg aaaagttttg actaaaatta acattaccga cggtcggaat ctgaaaaaca
    25621 gtttctgtca gagtcacagt atcaagattg ccgctaaatt gaaaatagag ttggtcttta
    25681 gtcgaatcac cgtaaatatt attatccgga ttgaccagat cggctaatgg caaagaaaca
    25741 tcagccagcg gcagatttat cgtctgataa taagtcggca tttccggcgg cgccagcggc
    25801 tcataggaac aaccgcaaat caatccgatg ctaagaaaaa aaatagtcga atagagccta
    25861 aaatgcttta ataaaaatag tcctttaata aaagataaaa tccggatcaa ttttagtacc
    25921 cagctttatt tcttcttgcc accacgcacc agataggtag ctagcgcaaa atcgaaacct
    25981 tgcattgaat gcacccaaag acctcgattc ataccaagcg cccagtcaat atgcagtgcc
    26041 gcaagattca cactactacc gatatcaaat tcccaaccac taatacctcc cagcgctaat
    26101 ccaaccctta acggcatttt gggaaaatag gcatattcaa aaccgaccgc tgtccgccac
    26161 cctttcgtaa gattcagccg atcgctaaac gacatgctaa aatcaatcgc agtcagcaaa
    26221 tctttgcgca attgctttgc cgcgcccact ttcagcacca gtggcacttg aacccgaaag
    26281 gcacttgaat cctgtttaat taatttagta tcgctgaaat gatcttgata agtattttca
    26341 ctgcggaaaa gggcatcggc atctaagctg tcaatccgaa aattgtagaa gtaactctca
    26401 ccctcgaaat caagtttcag gtcggtattt tttactggaa aacttttgat ttgaaatccc
    26461 atcattttta aaacatcaat accattgtct ttgagccatt ttgagagcgc cgtttcggta
    26521 ttccatttaa ccgattcacc cagattcaaa acgctgaagc ccagagtcca gccgtttaaa
    26581 tcttcaaatg cgacacctag gtctgcccca tagccgcgac catgactgga ctcgcgaaaa
    26641 taatacgagc cattaccgta aagcacaaag tcactcgtgt ccaccgcgat ttggccagta
    26701 ctgtaagcgg gatccaatcc ataataaccc agaccttgca aatactttaa agaaatccca
    26761 accgacattt tgccgaaagg aatccctatc gaataaacat actcgacggc agtcatagca
    26821 tcgcatcgca aatccaagtt ataggtcgtt cccttttccc aattgccgaa aatgacatcc
    26881 agagccggct tggaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatcg
    27001 cattaccggc tccacccaga gcgcggttaa tcgggctatc ataatactga gcatcgagcg
    27061 gtcggggcaa tccgtagaga aaaaacccaa gcagtatcaa ctgccacaac caatttgatg
    27121 atcttaagat tcgggtaagt cgtttactaa gcttcttcgg cttcattttc agtcagaatc
    27181 ttttaaattc gccgcaacaa aatacaagtc cagaaagccc ctgacatgaa ttgactgcgt
    27241 ggcggtaatc gttgccagac tcgtatccgg attaaccaac ttaaaataag gtttaaagta
    27301 acacgtatcc gctaacaatc caatcgcatc agaactgatc tgaaaaattt gacttttgat
    27361 tccgggtata attaaagtgt tctcgctaaa ttgagcagag ggaaattcca acaccgccaa
    27421 ggtatcaatt tctaccacgg ttgtatcgtc cccaaggtgg cagatagttc catcgaaaat
    27481 aacaaaatcg cttggtaaat tgcctccaag cgtatcaaat ggaaaaaaat taaaattact
    27541 tattaaaaaa taaaggtcac cttgcagttg tgtataattc tcgacggtca tatccagctc
    27601 accaccattt tgcgacgtta ttaaccgagc gcgagttgtg ctgtctttaa taaaataggt
    27661 agttgtatta ccggaagcta gtgtaatctg cggcggtatt ttaaacctca acggaaccga
    27721 tatattatac ttgacgttca cgcccgcatt aaaaacagta ttgcgctcaa aatccgccgt
    27781 ggcagtcatg actttgacaa aaacccggac ggtatcagga agattggata caacctcacc
    27841 gatatccatc tttaaatccg ctcgatttat cgctatcata gtgtcagaga ccgtttcaac
    27901 accatgaact ttctttccac tcaaatggac ataaaccgaa tcaaaatctc taccgaaagt
    27961 ttcaatattc agactcgccc gggcactgtc aaattcaata tcgctcgctc cggttagact
    28021 tttcgctacc agagcgggtg gcgtctcgcc tgtcgcaaac actcgcccgt taaatgattc
    28081 tagagctaat tggccaactg tcagcgaagt cgtcagtttt tgtttgagac tactggtagc
    28141 ctccgctaat tcgcccagca cttggacttc gatattaaat tccaacgaat caatctcttc
    28201 ttttaataag cctccgatca acgaagcgcc acccagttcg atcattatac tattctgtaa
    28261 actattaaca ggtaaatcaa gagtagcgac tactgattga cctggatttt tttgaaaatt
    28321 cggagctatt acgcgaatct gaatcgggtt gggaaaagaa tcgctcaact gtgccgccag
    28381 taccaaagcg ttaatcggaa tattgtcggc agtgaaaccg gtcgaattgt ccagaacacc
    28441 agaagtaact cggttattag aatcgtccca ggcttttttc attatataat taatcggatt
    28501 gaccgtgttg ttgacattac gtttgatatt ttttcttaag tcaagcgtct gactgatagt
    28561 catagccggt acaatccgga aggttgtagg attgatctcg atcggcatac tggttgaatc
    28621 agtaaacgaa taatatagaa caccactttg atcggcttta attggattgc cgttctcggt
    28681 atcaagcaaa tcctgaaatg aataggattt atctattaag ggtagaatta ttttagaatt
    28741 ccattttgcg ggaagactcg gagcagttgg aattttaaac tcacatttgc ccagccagat
    28801 taggagggtc agaaaaagga taaaccattt cgaccggcaa tgcgtagttt aagttccttc
    28861 gctataggcg gttttatatc gaacttgctc tggggttaaa gtatcaatgt tgacacccat
    28921 agtttgtaat tttaagagcg caatttcctg atcctgctcg acgctgatgt cgtaaacttt
    28981 ctttgctaat ttggctccat tttgagctag gaaaatcatt gtcataaatt gattagcaaa
    29041 ggacatatcc attacttccg atggatgccc ctccgccgct accaaattca ccaggcgtcc
    29101 ttcacccaga acatagatcg aacgaccgtt ggttaattca tgcttaacgc aattgggacg
    29161 catttcttgt ttcgttttgg taagggtggc tagctctttc aggttaattt cacaatcgta
    29221 gtgaccggtg ttgcagacga tagcgccatc tttcattttt tcaaaatgac gactgacaat
    29281 gacatccttc attcccgtag ctgtaatgaa aatatcgccc agctcggcgg cttgatccat
    29341 cggcataact cgaaacccat ccagtttggc tttgagagcc gcagttggtt taacctccgt
    29401 gacgattaca ttggcgccca tgccgctggc acgtttggca acgccttttc cgcaatgtcc
    29461 ataacccgcg acaaccattg tcttaccagc ataaagtatc gaagtggcgc gcaaaattcc
    29521 gtcaattgag gactggccgg ttccatagac attatcgaaa tcccatttgg tttcggcatc
    29581 attgacagcc accatcggat agagtaattt tccatcctca gccatagctc gcaaacgatg
    29641 aacaccggtg gtcgtttctt cggaaccgcc caggactcca gccgccagtt ccggaaattt
    29701 attatgaata gtaaaaacca gatcggcacc gtcatcaagc gtcagattgg gtttttgaag
    29761 taaagtttgc tcgatacacc agtagaattc agcggttgac aaaccgtgcc aagcccagat
    29821 tggaacacct tcttcataaa gagcggcggc gacatcatct tgtgtactaa gcggattgca
    29881 accgctccag gctacttctg ctccagccgc tatcagagtt cgaatcaata cagctgtctc
    29941 tttggtgaca tgtaagcaac cgctgatacg catccctttc agcggcttgg aatcgcgata
    30001 tttctgcctt aacgccatca taaccggcat atgcgattcc gcccattcta ttttcaactt
    30061 tccagagggt gccagtgtga tatccttgat ttttccagtc attactttac tccaaaatat
    30121 tgctttaatt cggcgacttt gtcggttttc tcccaagaaa attcttcatc ttcgcgaccg
    30181 aaatgaccat aagcggctgt tttgagataa atcggacgtt tgagacataa atatttaatg
    30241 attgccgccg gttttaaggg gaaaatcgct cgaacggctt ccgctaaacg ttcttcaggc
    30301 tgtttaccgg tgccggaagt attaatatag accgaaatcg gatcagcaac gccgatagaa
    30361 tacgccagct gaacttcaca gcggcgggct aaaccagccg caacgatatt cttggcaatg
    30421 taacgtgcca tataggaagc cgagcggtca actttggatg ggtccttgcc ggagaatgct
    30481 ccgccaccat gccgtcccat accaccgtaa gtgtcgcaaa ttattttacg gccggtaacg
    30541 cccgagtcgg cttccggacc gccgatagca aaaatgccag tcgcgttgat ataaaattta
    30601 gtttcaggag taagatatcc ctgacaaact ggtaaaatca catgattttt aatatccgcg
    30661 acaatcttct catgcgcaat ttcgggatta tgatgtaccg ccaccactac attggtaatt
    30721 cttttgggat gatcatcttc atattcgacg gtaacctggg acttaccatc aggcctgacc
    30781 cagttgagta ttcctttttt ccgaacttct gccagacgca tcgtcagctt gttcgccata
    30841 ctaatcggta aaggcattag ctccggcgtc tcgtcgcagg cgtagccgaa catcaaaccc
    30901 tggtcgcccg ctccttgctc gtgtttctca gtttcattca cgccgagggc gatattttca
    30961 ctctgttcat gaatagatgt tatcacagcg caggaacgtg catccagacc aaagtcggga
    31021 tcggtataac caattttttc aatggtttcg cgtactatcc gcggtatatc aacataggct
    31081 ttggtcgtaa tttctccgac gaccataacc aaaccagtgg tcgtcatcgt ttcgcaagcg
    31141 actcggctct ccggatcaac tgtcaaaagt gcatccagta tagcatccga aacctgatca
    31201 cagattttat ccggatgacc ttccgtcacc gattctgagg taaacgaata ttttttcatt
    31261 gtctctaact ccattattaa aatttaaaac gttcaaaaat actgtaatgt atcgagaaaa
    31321 tcaacttaat ttctgaaccg tataatcagc cgtatcaatc tgctgaacga ttccggagac
    31381 ctgacttttt tggccaatca ggctctcatc taatataacg tcctttaata tcgcgccgtc
    31441 cgaaattatg gcattcgata acactgaatt cattacctga acttcagccc caagatgaac
    31501 attcggccca ataatcgagc gtttgattac cgcatcacgg ccgatataaa ccggtggaat
    31561 aatgactgtc tctggaatag cgggtggcgg ttgcggattc ttgttgagta aataggcatt
    31621 ggtcttaagc aaagacttac gcgtaccaca atcgaggtta tgttcgatgt taattacctc
    31681 gaacgattcg ccccattcca gcattttctg aagggcatcg gtcagctggt attcgccttt
    31741 agttttaata ttgacggtaa tgatatagtc aatagctttt ttgagcaatg cgcctctttg
    31801 aattaaatag atacccgcaa ttgccagatt gctggtagaa ctagacggtt tctccaccag
    31861 gcgagttact ttcccatctt tcatctctac tattccaaac cgacgtggat catcaaccgg
    31921 cataacgcca atcaggtttt cggagcgact gctcaaccct ttaaaatcaa gttccaagat
    31981 aacatcccct aaaataatca tcacaggtcc atcctcaggg gttaagccta atccgacggc
    32041 gtgtcccagc ccacactgct ccaactgctc aatgaaagta aaattaaagt cgtaattctt
    32101 taatgagtag tcgcgcactt gctccccgta atagccgatt atgactgtca cgtccttaat
    32161 tccggtcaag gcaaggcggt cataaatatg gccaattatc ggtttgcctg ccacaggcag
    32221 gagcactttc gatcgggtta aagtcaatgg cctaaggcgt tcgccaatcc cggccgcagg
    32281 aatgatagct ttcaaaatcc aacttccttt ctattccaaa tcttttttca gataattctg
    32341 taaatcactg atttgagttt tttgccgaat cgtggcggca attttcttct gaaagttaag
    32401 tgcggcgttg gtgcccataa ttttcagaac atgattcaaa gcaataacct cggcaatcac
    32461 tgtaatattt ttaccgggat aaatcggtag attgataatc ggaatatcta catcgagaat
    32521 cttgttgtat ttttcttgag caccaatcct ttcgaagttt ttggtattat cccattcttc
    32581 gagttcaatc tggacttcga ttctcttctg ctgacgaatt gcccggatgc cataaatatt
    32641 ccgaacatcg acgattccca gtccgcgtaa ttctaaatgg tgttctaaaa tttctactcc
    32701 actaccgata atgatattat ccgcccggcg aataattttg acgacatcat cagccaccag
    32761 ccggtggcca cgcccaacca gatcgagagc aatttcactc ttaccgatcc cgctcggtcc
    32821 ggtcagaagc aagccgattc cataaacatc cacaagactg ccgtgaatta tcgttgaagg
    32881 cgccagctcg cgatccagat aatcaaggag agaagccacc aataaggtcg tcgttaccgg
    32941 cgtcgctaac agagccacct gatatttacg agcgagccta acggcacaaa ccggtggttt
    33001 aacatcatga gcaaagatca tcagcggtat tttagctgaa aaaaaatgct cgaaaatttc
    33061 ttcaaccttt tctcccgaaa gagttttgat ataacgaaat tcaccctctc cgatcaactg
    33121 gattcggtga ggatcaaaac tttcccagaa accggaaatt tccaatcctg gacggttcaa
    33181 tttagatgaa gttacgaccg tatcgtgacc aatatcacca ttgatacgta aaaatcccaa
    33241 cttcttgcga ttttcatgat atatccgtcc gactgtcaaa tcattctgat tcatcgcgca
    33301 tacctcttct ctttgaattt tttcaattgc ctttcaattt ttgctatggc taaatcaatt
    33361 gatttgatca tatcatcact tgcgacctgc gcgatcaggt ctttgccgcg cacatgaata
    33421 gagatttcaa tagattcgcc gtccttagtt ttctccataa tgactttaca gctggtaatg
    33481 aggtcttcgt aaagtttaag attgttcatt tcctcttcaa gatgcttttt ggcacgatcg
    33541 gtaatttcat aatgacgcgt ggttagttcg atattcattt gaaacctcct ttggctattt
    33601 aaaattgatt atccaattca aaccgcaatc aaagggaaat gcggcattgg ataatttttg
    33661 ggttaaatat tttaacggta ggctattcat ccgactttta tttcttacct gaatccgcgt
    33721 gcggatgagc ttgttgataa acttctttca tggtgctaat gcggacatgc gtgtacaatt
    33781 gagtcgttga taaactgctg tgccccagta aatctttaac tgcattaaga tcagcaccat
    33841 gatccaataa atgtgaagca aacgagtgcc ggagaacatg cgggctaaga tgagtttgct
    33901 cggaagcttg ttgtaataaa ccgctgatta acttctgcac ctgcgatcgc gaaattcgtc
    33961 ggtagccggt aggttgagaa tttggtccgc cgctactact gataaacagt ggcgagttta
    34021 attctaacgc gcctacttcc tttttccgca tattaagata gtcattaatt acttcggcgg
    34081 cttttttacc aaacggtact actcgttctt tagcgccttt gccgaaaaca cgcagtgtct
    34141 gccgactgaa atttaagtca ccgacgttga gcgctatcag ctctcccagc cgaatcccgc
    34201 tactgtaaaa taactcaagt atggcgcgat tgcgcttgcc gaccaaggtg ttatcatccg
    34261 gcaattcaat agctttggca atttcctctt cggtcaaaac gaccggcagg cgtttttttg
    34321 ccttgggtga aagaatcaaa gccgccgggt tgctatcaac ataaccctga cgggtggcga
    34381 atttaaaaaa ggctttgagc gaagccaatt tgcgagagat ggaactaatt tcgagtcctg
    34441 actctgataa tttacccaga taatgacgaa ttgccatttt cgatacggag ttgagatcga
    34501 ttggcacctg attggcatat tcagcataaa atagaaaaaa ttgttccagg tcgcgacgat
    34561 aagccatgat ggtattttca gaatatccac gctcttgcgc taggaatcgc aaaaaattta
    34621 tcatcatttc ttcaacagta atcacagctc gattcctgct tctaatatat tcaaaattct
    34681 gctaaaatca tcaggtaaag gtgcgattat ctcgatgtct ttatcaaaaa atggatgttg
    34741 gaatcttacc aaataggcgt gcaataattg acggtgaacc tcagactgga cttttttgag
    34801 taatgacaca tccttagcgc taactgccga tccctggcgc agtttacctc cataaaccgg
    34861 gtcgccaact actggatgac caatggattg caaatgaacc cgcgcttgat gagtacgtcc
    34921 ggtttccaaa accaccgcca gataagccat aatacggtaa tctttcagaa tagtaaattg
    34981 ggtacgcgct gggcgacctc gtttatctat ggtgaaaatc tgacgattac gctgagaacg
    35041 acgtagcgga gcgtcaatta ttccaacagc tgtttccgga actccataca caattgcccg
    35101 atattgcttg aaaacctttc gttcggcaaa aagacgactc attttccaat gaacttcgtc
    35161 atttttagca acaatcagca ctccggaagt atccttgtcc aggcgatgaa ccagtcccgg
    35221 gcgctcactg ccgcccaacg aacttaattg attagtataa tgaagaagag cgctgaccag
    35281 cgtcccttgc cgaacaccgg cgccgggatg taccactata cccggttttt tattaattgc
    35341 cagcaaatat tcatcttcat aaagaatttc cagcggaata tcctccggct gaattgggct
    35401 gattggttgc ggttcagcaa aaacggcaat cgtatccata gcctgcagat gatagctagg
    35461 tttggagatt tgcccattga ctaaaatttt cccattttta atcaaaccgg caattttcgt
    35521 cctggaaaat gcaggtaatt tttcagannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35641 nnnnnnncag tgttgagtgt gaggaacttc ctccagtcgc tctttcataa tcagttctcc
    35701 acattcaata caaacgccgt attcgccagt cttaattctt tcaagtgccg agtttagatg
    35761 cgataaaaat ttattttcac gcgctaacca gagaaaggct ttttcgcgct cctgggagtc
    35821 ggttccgacg tcagccatat gataggcata agcagaatcc attgccgtat tgtcattgcg
    35881 agtattgtcg ctaacggtag tggcttccag ccgttcgatg gctttaagag tttcttctct
    35941 tttctttaag atgagattct tgaaatattc aagatcctct ttactccatg cttttttcgt
    36001 cgttttggct tccatattta ttttctccta ctgctcgaaa tttacttaat tcgtgtcaaa
    36061 gaaacacgaa ctttgttatt ttcgattttt agctcttttt catactcgcc gggcgcaaaa
    36121 ttatctacaa ctttttgcgc tagggtctca ttacagaaat actcgatata attatgaaca
    36181 gcggcggtaa aatcggctgg cgcttcaaag ttgacctgaa tgcgatcctc tacctgaaaa
    36241 tcggcttcct tgcgcatcgt ttgcacatgc cggataaaat cgcgcaccaa gccttcctgt
    36301 aaaagagacg gagtcaattt cgtatcgagg ataacagtga ttccgttact ttcggctatg
    36361 gcgaagccct cattttcctt gcgttcgacg ataacctcat cgcgtgatag ccaaattttg
    36421 ccatcaatta aattcaactc gaggttttca ctcttctcaa ggcgtcgggc gacttcgatc
    36481 ggattcagct cggcaattgc ctgccgaatt gctggtaaac gctgaccgta ttttgctccg
    36541 agctgggcgt aatttggttt gatttgaata ttaacaaact gcgtttcgtc ggtgataaaa
    36601 tctaaagctt ttacatttaa ttcttccaga atttgatctg ttaaatctac cagatcattg
    36661 gcggtaacct cagcaggcaa cttcacccac atttgcgcta aaggctgacg cacttttagg
    36721 ttggctttgt tacgcgccgc ccgaccaact gacacgattt caataatggt gtcaattttt
    36781 ttcagtaatg cctcgtcgaa ctcctcctca gcctgcggaa acttagccaa atgaatgctt
    36841 tcgggagcat ccggctccaa acctcgaacg agattctggt aaattttttc cgttataaac
    36901 ggcacaacgg gcgccaggat taagatcaat cctttcaatg ctttatataa ggtctgataa
    36961 gcgctccact tatcctggtc attgtccgat ttccaaaaac ggcgacgact gcgccgaata
    37021 taccaattgg aaagcacctc cagaaaatca tctacgcctt tcataacgcg agcgacgtta
    37081 taatcgtcca gtgcttcccg tgacaggcga atcaactgca ccaaccgcgc catcaaccaa
    37141 cgatccagtt cgcttagatt ttcatccgat acctgatgct tttgaggcga atatccatcc
    37201 aattcggcat aagttgcaaa aaaggaatag gaattccaga gtgtaatcag gcgcttgcga
    37261 taatcctgcg cgatattata accgaaattt aaattattaa acggattatg atcggcatat
    37321 aaccagcgca tcacatccac gcccattttt tccgcggctt catcaaacca gatggcattg
    37381 ccggcacttt tgtgcatatc ttggcctttt tcgtccttga ctaaagcata gccgagaatc
    37441 gttttgaacg gctcccgatt ttccaataca gtgctcatcg ccagtagaga ataaaaccag
    37501 tttcggaatt gacccggcaa ggattcacag ataaaatctg ccgggaacca ctcttcccaa
    37561 taagcccgat cagtagtgta atgcagagta gaaaatggta ctattccagc atccaaccag
    37621 ggatttccaa catccggaat acgactgact ttggcaccac agcgtggaca ggtaatctga
    37681 acattatcaa cccaaggacg atgcggcgag tgcccctcga atttatccca gccttccatg
    37741 gctttttctt tcaattcctc gcgactaccg ataacggtaa cctgaccgca atcttgacat
    37801 tcatagatcg gcaaagccag tccccagtac cgcttcttgg aaatcatcca atcgtgcata
    37861 ttacgcaacc agtccagttc atgttgtaaa ccatattcag gaatccaacg gattttgcga
    37921 gttatatcca tcattggctg acgcacgcca tccatggcga tgaaccattc atccactaat
    37981 cggaaaatca gctcggtgcc gtgccgccaa caaaccgggt aacggtgcag atatgaatcg
    38041 gtgcgatatt taataccttt ggcttccagg tctttaaaaa tatccctgct aatgccatct
    38101 acattgcggc cagttagcca gccaaagcct tctaaataat taccgaattc atcgagcgga
    38161 gccagaaccg ctaggtcgtg aattttacca agctcgtgat cttcctgacc gcatccggga
    38221 gcaatatgca caattcccgt tccttcgctg gcactgatct cttcccaatc aattacgaca
    38281 tgcttgacat tcttctgagc aggtaaatca tcaaacggac cgtggtaagt ccagcccagc
    38341 atctcccgac cgggaatttt tccttcaacg gtaaattcgc ctttaattac atccctttca
    38401 cgtccttcga caacatagag aatttcattg ccctgccgaa ttttcaagta attcaaatcc
    38461 ggatgaacgg cggcggcagt gttcgaggtt aacgtccagg gcgtagtcgt ccagactaaa
    38521 agcgattcgc caggacgatt gattagcgga aatttgaccg taatactcgg atgtgttatt
    38581 tccttgtaac cttccgtagc gatttcatgc tccgatagcg ccgcaccgca acttgtgcac
    38641 cagggcataa cgtcgtgccc tttataaatc caaccatttt catggcattt tttcagaaaa
    38701 agccagattg tataattgtt ttcatcggac attgtataat aggaattctt ccagtccatc
    38761 caataaccca gccgaattga ctgctcggtc tggattttcg aatattttcg cactcgctct
    38821 ttacaacgct cgacgaattt ctcgatgcca tatttctcga tatcacgttt tgatttaaaa
    38881 ccgagttctt tttcgacttc gacctcgacc cataaaccct gacaatcaaa accgttttga
    38941 tagcgagtgc ggaagccatt catcgcttta taacgatgaa aaatgtcttt gtaagtgcgc
    39001 ccccaggcat gatgaacacc catcggatta ttggcagtta tcggtccatc aataaatgac
    39061 cacttcggac cattagcatt ctgcgccact aattttttaa atatttgatt ctgctcccaa
    39121 aacttcagga tttcatgctc gatagccaca aaatctacac tggacgggac tttctttatc
    39181 actgagaata aacctcctaa cgccgaaaca aattgaacaa aatgaaaaat accagactaa
    39241 tgagcagaca ggtaacccac ggaaaataaa aagtaaaatg ttcccggcga attataatat
    39301 cgcccggtaa acgaccaatc ttatcaagaa atggaaattt tccggctcct gtcaaaagaa
    39361 ctccgactac aatcaacact actccgaata ttattaacaa tttgcctact tgattcattt
    39421 aattgctacc aatctccatt cctgatctgg tttaccgcta gcactttcca attaactgcg
    39481 aaatttagat ttaactggct aaaaaaacaa ttaaaatggc tcacttccta tttgaaaaaa
    39541 gaagggcttc cgcaagggaa acccttcttt tcggagagag aaaattgagg agttatttac
    39601 taaggtctat agtctcaaaa aaggtaacat tcttacgttt aatcagaatc agtacgatgt
    39661 ctgttttcac ctcttcgagg gctttctgat actcagccac gttgcttata tcccgttccc
    39721 caatcctttt aataatatca ccaacctgca aaattccagc ggctctacca ttccggtcaa
    39781 ccccagtaac cacgactccg gtttcatcag gactgatacc gtattggctg gcggtgactg
    39841 aattgatctc gtcgacgcgg ataccgagtt tggaatcaga ttttgtatcg gggtaaacac
    39901 cgcgctcgac agattcaggt aattcgccga gtttgacatc aatttttaac tcttttttac
    39961 cacgcagaac cactagggtt ttgggcttac caacttcgga agtggaaact agcgtgcgca
    40021 ggtgcgccga atccttaatt gtttttccat cgaattcctt aataacgtcg ccgactttca
    40081 aaccggcttt ctccgccgga ctgtctttaa caacttcgct gatgatagcc ccctgacgac
    40141 tttcgatacc gagcgatttg gcaatatttt catccacatc tccgatcacc actcccagcc
    40201 aggctcgaac gactttgcct ttttctatta aatcagtcat aactttctta gcaagattaa
    40261 tcggtatggc aaatccaatt cccacattac cttgtgacat tccggccgta gcaatagcgg
    40321 tattaatgcc aatcaattca gcccgaatat tgaccaaagc acctccacta ttgccgggat
    40381 taatagccgc atcagtctga ataaaagatt cataattagt gttattaatc actccgctac
    40441 ggcctttagc gctgacgatt ccagccgtca ccgtatgagc caggttctcg ctgaaaggtg
    40501 atccgattgc gagaacccac tcgccgacgc gtagagcatc cgaatccccg agaatcgccg
    40561 gttgtaaatc cttagcgtca atctttatga cagccagatc ggtgagagga tctgtgccaa
    40621 cgatttcagc tttaaattca cgattatcaa ttaatttaac actgattttt tcgcctcgtt
    40681 caactacgtg attgttggtc aaaatataac ccttttggtc aacgattact cccgagccaa
    40741 gcgccctact tcgcatctcg ccttgtggaa atccaaaaaa acgccagaat agatcatcat
    40801 ccggaaaacc cggaaaatcc cgacgcagat tagttttgat gactttttcg gtcgtaatgg
    40861 tcaccactga tggagtaact gattccgcaa tctgcacaaa agcatcgtta aaagcttgca
    40921 gagagggaat atcctcacca gccgttaccg ccgccggccg aaacgtcgtc attccggaat
    40981 tcttgtcttc ataaaataca accttgccag gtctaataat gatgctggca atggtaaatc
    41041 caagtgcaac cagtaaaatt ccgcctatta ctataccaat ttttcgtttc atatcttatc
    41101 ccctttttaa tttattttta aaaaactggt agattaaact aatctttttt tttattgttt
    41161 caaatactaa acaagagaat gtgctggtta tttgcatgga tgcatgattg aatgtactat
    41221 taaagtttga cgagaaattt agaaagtgct gttaagtcct tataagtgct tttaagtgct
    41281 actaagtcct caaaagagct tagaagtact taaaagataa cttatttctc ggagcaactc
    41341 cttacacctt tgcgtcacaa tttcagatca taaatatcgc atattcatct tgatttcctc
    41401 aaaatactta agcgaattga attcctcagc attcttagcg gtttgttatc taataatcca
    41461 ttttgcctat tttttatgca cttcgctaat tttattagga atatattggc tttaaaattt
    41521 ccgcggtctt aatttttcac cgtaatgagc cggatcaatc ctggtaataa aaaaatccca
    41581 aaagtcatcg tcgtcggtgg tggaccagcc ggtttgatgg cggcgggaca agcctcactg
    41641 tcaggcgcgc aggttatttt aatcgaaaaa atggaacgtc ccggccgcaa acttgccatc
    41701 accggcaaag gacgctgtaa cttgaccaat actaccgagc taagcgaatt cctgacccat
    41761 tttggacgaa acagccgttt cctgcgcggc gcctttcacc gttttttcgt gcctgaactg
    41821 ctggaatttt tcaagcagtt gggaattcca accgttactg aacgtggcgg tcgtattttc
    41881 ccagtccgca atgacgctaa tgaaatcgtt acggctttag tcaattgggc aaactctacc
    41941 ggcgttatca ttcgcccgaa tactcgcgcc aaccagctcg tccttaccga aaatcgaatt
    42001 agcggcgtaa tcgttaggaa tccaaattca actactgaga aaataaatgc cgacgcagta
    42061 ataattgcca ctggaggagc gtcctatccc ggcaccggtt ccactggcga cggctatcgt
    42121 ctcgctaaat ctgcaggtca ttccatcaca ccgattcgac cggctttagt gccattggtc
    42181 accgtgggca atcttgctac ccgtttacag ggactcagtc taaaaaacgt aggtattaaa
    42241 gtattcgccg atagcagaaa aattgccgat gaattcggcg agatgttatt tactcatttc
    42301 ggactctcgg gtccggtaat tttgactgtt agcaaactgg tggttgatgc cctaaatgcc
    42361 aaacaaaagg tgcggattag tattgatttg aaaccggcac tcgacgacca aaaactcgac
    42421 ttacgcttac aacgtgattt tgctcagcat ggtaaaatgc aatttaaaag tgtcctaaaa
    42481 gagttcctcc cccagaaact tattccggtt tgccttgacc taataaaaat tccacctgaa
    42541 aaaacttgca atcaaattac cacggccgaa cgtcgtcgtc tccgactctg gctgaaaaac
    42601 tttaacttcg acatcagcgg gcaccgcggt tttggcgaag cgataattac cgctggtggg
    42661 gttgccctga atgaagttga cagtcaaact ctgcaatcta aaattgttca gggactttat
    42721 tttgccggtg aaattctgga cttagacgcc gacaccggcg gctataatct tcaagccgct
    42781 ttctcaaccg gatggctggc gggacaatct gctggacgtt aattactccg tgatcattaa
    42841 aaattatccg tacagaagag tcgaacttgt tatttaagga agaattgctt taatctcacc
    42901 agtttttcct cgatgcgatg tggcttattt atacttggaa aaaatatcca aaatatatcg
    42961 catttttcgt ggttgcaaag tttcgaatag gattttcagt tcaatcgttg gaaatttagt
    43021 ttaatgtttt tgcccactac taaacaggaa atggaagaac tcgggtggca ggagctagat
    43081 gtaattctgg ttactggcga tgcttacatt gatagcccgc acattggcat tgcagtaatt
    43141 ggaaaagttt tacttaaagc tggttatcga gtcggtgtca ttgcccagcc cgccctgaat
    43201 gttcctgacg acattatgcg cctcggcgaa ccgtgtctct tctggggcgt gaccggtggt
    43261 tcgattgatt cgatggttgc caattacacc gcttctttga aaaaacgccg ccatgacgat
    43321 tatactcccg gcggaattaa cgaccgccgt cctgatcggg cggtaattgt ttacaccaat
    43381 ctcattcgtc agcattttaa aaaaaccaaa ccgatcgtgc tgggcggcat cgaagctagc
    43441 ctcagacgga tagcgcatta tgattactgg agtgactcca ttcgccgctc gattcttttc
    43501 gatgccaaag ctgattttat cacttacggt atgagtgaac gctccattct ggaattggcg
    43561 gaaagtcttc agaaaggttc cgattatcgc gacattcgcg gtatctgtta tattgctcct
    43621 cacaaaccgg aaaatacttt ggaactaccg tcttatgaaa aggtgcgtga caatcccaaa
    43681 acttttaccc gtatgtttca tgaattttat ggaaataatg acgccagaag tgctcagcgg
    43741 ctttgccaga aatacgccac acgctggtta gtacataatc caccgtcatt gccgctttcg
    43801 caggctgaac tggatgaagt ttacaatctt aattacgagc gtgatgtgca tccttattat
    43861 gccaaacaag gctcagtcaa agctctggaa acgattcagt ttgctgttac cacccatcgc
    43921 ggctgttacg gcgaatgcaa tttctgcgca attgctattc acgaagggcg cacggtacaa
    43981 tggcgcagtc cggaatcggt caccaatgaa gtccggagtt ttgcgacaat gcccaatttc
    44041 aagggaatcg tcgccgatgt tggcggtccg actgccaata tgtacggctt cgaatgtccc
    44101 caaaaattga agaccggcgg ctgtaaacaa aaacgctgta tttatcccga aatctgtcct
    44161 caactaccag ttagtcataa gaaacaaatt gaattgctca ttaaattgcg tgccttaccg
    44221 ggcgttcgca aagtcttcgt tgcctcggga atccggcatg atttagtctt ggctgataaa
    44281 aaatggggcc gaaaatatct acgtgaagtc atctccgaac atacttcggg tcagatgaaa
    44341 attgccccgg aacacattgt cccgcaagtt ttgcgtttaa tgggtaaacc taatcatgaa
    44401 acactgatca atttcattaa attgttttat aattataccc gagccgcgaa caaaaaacaa
    44461 ttcctaactt attatctaat cgcggcacat cccggctgca cctatcaaga catggttaag
    44521 ttaaaatcat ttgcgaccaa ttttatgaaa attacacccg agcaggtcca gatttttctt
    44581 ccggcacctt ctacttattc agctttgatg taccacaccg gcttcgatcc cttcaccgct
    44641 gaaaaaattt tcgtcgccaa aagcctccat caaaaagaaa tccaaaaaaa tgtcgtaacc
    44701 tcaaaaatga aaaatccaat attaaaatcg aaataaataa ctttatgacg gataaaagat
    44761 tagaggaata tcggcaggca ttaattgatc tgagcaatgg taaaactccc catccaatta
    44821 actgtgaacc ggatgatgaa ttatccgcgc tcggtcgcga gattatccgg ttacaaaaag
    44881 aatggcaaga attgtctctc ctcattggtt tgaccgccca gatcaacgcc ggcttgaatc
    44941 tggaagaagt acttgaccgc atctttgatt catttcacgc cattattcct tatgaccgca
    45001 tcggagtggc tctactaact gatgacggca aaaatgtccg atcttgttgt actcgtagtc
    45061 gtctcccagt tattaaatta aaagaaggtt actctgcccg cttgaaaaat agtagcctcg
    45121 aacagatcat tcgcaccggt cagccacgca ttatcaacga tcttgaagaa tatttagcgc
    45181 aaaaaccacg atcaacctct acgcgtctga ttttatccga aggaatgctc tccagtctta
    45241 cctgtccgtt gattattcag acaaaaccgg tcggctttat atttttttcc agctgtaata
    45301 aaaataccta tcgcgatgcc catatcgaac tttttcagaa aatcgccgac caggtttcgg
    45361 ttattattga aaagagtcgt ctttatcaga atctagcggc tttaaacgag gaaaaaacaa
    45421 aatttctcgg catcgccgcg catgacttac gcagtcccat cggagtcatt aaaagttata
    45481 cagatatatt actggccggg ctcgtcggaa atttgactga ccaacagacc aatatcatca
    45541 aaacgatcga aaataactgc actcgtatgc tcaatctaat caatgacttg ctggacatta
    45601 gcgcgatcga atccggtaaa ctgacccttc aaaaaacaac aatcgagccg agcatttttt
    45661 ttgctaatta tgctgaaaac gctatgcgtt tagcggcggt caagtcaatc cacttcacaa
    45721 cattcatcgc cccgcaaatt ccgccgatcg aatttgatcc caaccgcatc acccaagttt
    45781 tagataatct cgtctcgaat gctatcaagt tttcttcacc agaaacgaca attactttgg
    45841 cggtcaaagc tataggtaaa aatttagaaa tcagggtagc tgatcagggg cagggaattc
    45901 cagccgcgga aattcctaaa ctcttcaaac cgttcagccg gaccagtgtt cagccaaccg
    45961 gcggtgaaaa aagcaccgga ttaggcttag caattgtccg ccgcatcgtg caagcccatg
    46021 gcggcaaaat tcgagttaaa agtcaacctg gtcagggaac cgaattcatt atccacctac
    46081 catttatctc taagtctgat aattaaactt ttaatatcat caccgcatct cagcgatata
    46141 acaatccaat aatcctctat cgtaagcaat tgaaaattaa aggttactca aaattacata
    46201 ttggacaatc tcaagaaaat ttcagatttt aaccgaccaa tattttttat tccatacctt
    46261 aaataatatt aatatatttt gaccattgaa ataaggaaga actatgagag aattgaaatt
    46321 taaaccgggt gacattatta ttaatgaagg ggcatacggc tcggaagctt atatccttaa
    46381 agccggttca gtcgaggtat ctaaaatgac cgatcacaaa aaagtcattc ttgctatttt
    46441 acaaccacaa gaaattttcg gcgaacttgg tttaattgac gacaaaccac gttcggctac
    46501 cgtcatcgcc aggactgcgg tgactgttga tgaaattaca cgcgaggatt ttagcgccct
    46561 gcttgacgat aaaatgcctt tcgttatccc aattatgcgg tcatttttcg aacacctgcg
    46621 ccaggctaat caactggttc tccagatgga ggcgcaaatt acagaagcgt cgaaaactgc
    46681 ccctcaacca aatgccccga ccgaaattaa actcgagggc ttaaccccaa ttgccagtca
    46741 atctcttaaa aatcgtacgt taatattacg aaaattcccc tttaaaattg gccgtgaaag
    46801 tggacagcgt cgcgaagatg tttttattga taacgacttg tccctaattg acaacatccc
    46861 ctataacgtt tcgcgtaatc atatgtcaat caatttttat aatggccaat actacattct
    46921 cgaccgcggc agtagcctgg gtactattgt caataaaatt ccgatcggtg gccgtctttc
    46981 caatttcaaa gctgaacttc ataagggcga aaataccgtc attcttggaa ctagcaattc
    47041 tcaatttcaa tttaaaatta cgatcgaatg attccccgca aaaacctcgc ccgggctttc
    47101 caaaaggcta tcgaacagcc tgactatgca ctaaataact tcatccatag attaaaagcg
    47161 tctttttcgt accattttct aaatggcaat agtctcgggc cagaaacaat ctcccttttt
    47221 ctaacctaca aatgcaattt aaaatgcgct atgtgcggtc aatggggcac caatggcgcc
    47281 ttccgcgaat atccaagcga agtggttcgt cagcaacttt ccctcgacga aatccagaat
    47341 ttgattgacg acgttcggca ctacaacccc aacatcacac tcttcggcgg tgaaccgatg
    47401 ctttacccaa attggctgaa agtcgtcgaa atcgtcaaag cggccggatt gcgttgcaat
    47461 atcgttacca atggcactta catcggaaaa tgggtccgtg aaattgcgga ttcgaggttg
    47521 gacgagatta ttttttcact tgatggaccg gctgaaattc acgataaaat ccgcggcgtg
    47581 cccggcacct ttcaaaaagc catcaatggc ttacaagaat tgaacacttt aaaaaaaact
    47641 cgcaacttaa atagaccgct cgtgaatata aattgcacca ttaccgaatt gaattatcgc
    47701 tcgattgatg agattattaa aatcgcggag gacattggcg cttgtggatt aactttacac
    47761 catcttcttt tcctggatcg tgcgacagtt atggaattta ttccgttctt tcaggaaaaa
    47821 tttggccaga cgccgaccga ttggctcggt tttatcgtgg acgaactacc gcaaatcgat
    47881 cccgaatatc taattgccaa acgtaatgag ctaaaaacgc gacggtttga taccgacgtt
    47941 aatttctatc ccaatttaga agataatgaa attcgccaat ggtacaccca attccaattc
    48001 aaagcctctt catacaaaaa tcgctgccaa agtctatggg cgacggctta tgtttttccc
    48061 gatggctctg ttcgtcctta tcactcgatg aattttacac ctggtaatat tttacaggat
    48121 aaattctcaa aaatttggaa caatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48241 nnnngaattt tacacctggt aatattttac aggataaatt ctcaaaaatt tggaacaatc
    48301 caagttacat cgcctatcgt aggtttgtaa ggaaaaacaa gtgtttttca atctgttcaa
    48361 aaggctgcac tgaatttttt cgatattgat attgataaag tataattaaa ttgactaaga
    48421 atacaataat aattaaatta acttaaaatg agacaggttt atggaaaaaa tacttatagt
    48481 agatgatgag caggaaatag ctgaattatt agaagaagta ctggagcaac tcggttataa
    48541 aaccgcaatg gctttctcaa aagcacaggc gtccgaagtc ctcagagaat ttgaacccag
    48601 aatcgttcta ctggacattc gtttaccaga cggtgatggc atttccttcc tgaaagaaat
    48661 caaagccagc caaccagccg tagaagtaat catgataacc ggattagctg atcgtgatat
    48721 cgccatagaa gctctgcgcg aaggagccgc cgattacatt tgtaaaccaa ttgatcttga
    48781 ttacctagca aatagtttac tcgctaatat tgtaacccgc tacaatacgg acgctccagc
    48841 actctagaaa tctgagaggt aatgcttatt ctcagcattt tttataaaat cgggtaaaaa
    48901 aatgaaacga aatcaatttt tagaattacc caaaaaaatt attttattgg tttgtataac
    48961 cagcctcttc agttttgctc aaaaacgtga accgtccatc accaccgatt cctaccaaaa
    49021 tgaaatgacc ctgtcacccg gcgatcgaat aaaagtctct tacattgata ttgggcccca
    49081 aggtgttcct atcgaaaaaa gttcgattct cgaggtgcgg cacgacggca ctatttttca
    49141 tgaattgctt aaagtcgttt atgttcagaa cctgaccatt aaacaggtcg aagatttatt
    49201 aactgtaaaa ttttcggaat tctttaccca accgcaggta gtagtcagta tagtcgaaaa
    49261 aactacgatc aaggtttttc tctggggcgg ggttgacaat ataggtgtat atcgtatatc
    49321 gcctggaaca actctcgccg aatttataat tacgcatggt ggcttaacca gcgatgccaa
    49381 catcagcaaa attaccttaa cccgtaaaga tggttctgtc atcattttcg atcttgaacg
    49441 atacttgttc agtaacgatg aaaccaataa tgttgaattg catgatggag ataaaatcat
    49501 cgttccgcga cttataatga aagacgagta cgctcgcctt tctaagggat atatcctgca
    49561 ggctggaaac gttctggaaa tcactatcaa tgaattagca actatggaaa aaagtccggc
    49621 aaccccggaa aattatataa ttgaccagga cggtaatatt tatcatcggc gctttggtct
    49681 agttcaattg ggaggtttaa ctgttgacaa agctcaggct actctgaccg aactggcaaa
    49741 aaaatattat cgtgagccgg tggttacaat agatgttgtc aaacttactt ctcgcagtgt
    49801 ctttgttttt ggagaggtta accgaccggg aatttatccg attgaaggta atatacgact
    49861 ggcggaattt ctggctgata ttggcggttt aacctccaat gctgacccac gtaaaattat
    49921 agttactcgc gaaaactata aaccggttac ttttgatctc aatgaatacc ttttcgatcg
    49981 aaaagatgaa aaaaatatct atcttgaaga tggggatcga attatagttt taagtcgtaa
    50041 acgaggattt attgtcaacc tagcggaaaa aatccaacca ttcaccactg ttctctatat
    50101 tatttcaacc gcacttaata tctatttttt aatcacatat attaaataga aatgacctgc
    50161 ctatggcaca ttatgaatta aatctgcgcg attactggcg aataatcaag aagcgaaaga
    50221 ttatcattat tgttactctt gtattgctca cctgctttag cctgattttt gccataatga
    50281 atcggcctac tcctctttac cgcgctaccg ccagcttgaa aatagagaaa agtaccaatt
    50341 taaccggtct ctatatgtac agtatttcct ggggaactgg tgatgacctc gctactcgcg
    50401 ccgaagaaat aaaaagctac ccaatgatag aaaaagccgc ccagaaatta gggctaattg
    50461 attcatcttt gacttctgag caaatccgca gtaacaaaga atacctcgat ttagtatcaa
    50521 gactcaaatc gcgcgtgaaa accgagcagg aaggttacac caatattatc aatataatag
    50581 taacagatta cgacccagtt cttgccgcta atctagctaa tgccctggct caggtttacg
    50641 aagtcgaaag cttcaaagaa aaaaacctcc aggctcaaaa agctctggaa gccgtccgta
    50701 accagcgcgc tatggctgaa caggctttac gtatctccga gcaaaaagtc caaaattacc
    50761 gtgagcaaaa taaactggca actctcgatg gagcggcatc acgtttaact gctgaattaa
    50821 actctgccga aactaatttg gaaaaaatca gaggtgacat ccatcaaatc gacaacattt
    50881 tacgtgaaat tgggaataac cgcgaatata tttatctcgc aaacttaaat ctgcttctta
    50941 gccagcctaa tcgtttcctg gaagatttac aaactcagct taatcaaatt cgttcccaga
    51001 ttaccaatta ctcccaatat tacacccctc aacatccagc cattattgag cttcaacagc
    51061 aattaaaaaa cactgaaaac agatttattc atgaattaga ggcgtttcgt caaaacttag
    51121 cccgagccga aactatcgcc gaagaaaaat ataaaagatt aaacgaagaa tataagtctt
    51181 taccattaat tgaattgacg ttgactaacc tcgagcgcga acgtgatgtc aatacccaga
    51241 tttatcagca gctcgaaatc cgctaccagg aagccataat tagatattca gaacaagtca
    51301 aggaagtctt tatcattcga ccggcttttg tgccgacttc acctatcaat cagactacag
    51361 tcggtccaac cacagctatc ggtatgatca tcggtttaat tctcggcatt gtgctggcat
    51421 tcgtcgccga gacactggat acgaccttca ataccgttga cgatattaaa gaagccctta
    51481 acaccaacat tatgggaatt attcccttcg ttgattttca aggcttaaag tcccaactag
    51541 ctgaaaaagc cgaaactcca gttcctgatg aaatccttga catgcaagcc aggcttgttt
    51601 cctattatag tcccaagtcg agcattgccg aaagcttccg aagcttgcgt accaatgtgc
    51661 attttgcttt attggataag ggttataaga ctttgatgat tacgtcgtca atttctaatg
    51721 aagggaaaac cagtatagcc gccaatctgg ctatttctat ggcccaaatc gggatcaata
    51781 ctcttttagt tgacgccgat ctgcgcaaac cacgtatctc gaaactattc ggaattgatc
    51841 gcgaaccggg cttaaccgat gtaattttgc ggaaagaacc gatagacaat gtaattcgca
    51901 ctatgtcgga tttaatggtt ggaactatgg catgcgatat acttcggact gataacattg
    51961 ccggaattga gtatctgaat attttaacca gtggaaaaat agagcgcaat ccagcggaaa
    52021 tcgctgcctc taagttaatg gacaatttaa tagctgaatt aaaaagacgt tatgatctgg
    52081 taatattcga tacttcgccc cttattccag ccaccgattc aacgattctt ggaacaaaag
    52141 ttgatacagt gttgcttgtc tattatcagg gaaaaatttc gcgcggcact ctacgacgtt
    52201 ccaaaagcca actagaacta cttaaaactg atattctggg cgtagtcgtt aatggtatga
    52261 aagccgaact aagtgccgat tatgccgatt ataaatatgg atatgaatat cactatcaag
    52321 aagaatctgc cagaccgaaa aacaaagtaa ttactgcatt agaatctttt ttcttgaaac
    52381 cagccgtagg aatgaccaac gggtttttca gtcaattaaa taaattgagg gctggtctag
    52441 ctcttattgc gcttggttta atcattggcg gtggtctatg ggcatttacc aaatatcagg
    52501 ggaaaaaagc aaaatcccag gtagaaagca aaataaccga aactatcatc ccgcagacaa
    52561 cttcatccag cgatacctca gtagccagtc atattctgtc ctcagacact ctatcgaacc
    52621 tcaccgccca aactgccccc attattccgt cagaattaaa acaactccaa gaacaatatg
    52681 gtatcgagac catttctcaa cagcaggttg accaaaattt gactgaaagt ggactactaa
    52741 ctaataattc tcccacaaca gttcaatcca ctataccacc ggctacccaa tctgacaagc
    52801 aaacagaacc gcttatcagc attgtctatt catcaactat tttgtcaaaa actgatccgg
    52861 ccgcctcagt tcaaccatta ccaagttctc ttccttatac tttaataatt gaacaatctt
    52921 tcgataagtc aactctgatt aatcaattaa ataagcttcg acggaacggg ctctcagctt
    52981 ttattactat agattttagc gatcctaaac aaaaaaatta cttactgtgc tatggcagtt
    53041 tttcatctga aaatgaagca attaacaaat ccaaagaact tcaattcctc ggattccgtg
    53101 gtactttcag aacaataaaa ttaccgttca gcatattgct tgatgatttc cccactcttc
    53161 aatctgccca gcaagctata gaacgtttcg catcaattaa agattaccta tatatacaac
    53221 catccagtca aaaaccggac gccaaaaaga cctattacct cctttttggt ggtttcccta
    53281 gcactgaatc caccgaatta gtaatgaata cctacccgga attaggtcac aaaactatcg
    53341 ttatacgcta aaaagagaaa aaatgccccg tcataaacac cataaatcca gaagcaatag
    53401 aaaggcaatt atttttctgg ttattccaat ttcactaatt atcgctataa caatcattat
    53461 aataattcgt gaaccaaatt ggccattcct cagcatttta cgtcaggatg agactgctaa
    53521 tcaagaaaag tacatgggca gtgccgtacg agtagaaact cccattcgac aaaagtacga
    53581 gactgaatgg cggaatgact actttgagac ctggcaaaaa acttacgaaa aaatattaca
    53641 gaacactgat aaaaacgaga ttaaagaact cgagcgcgag ttaaaagcga gcgaaaaagg
    53701 cattaattag tatgagcctg attgaggcct tcgaaaatcg gataaaccgg ttatttatat
    53761 ttctaagtat acctctttca atgcttgttg ctctcgtctt agtaaaagtt gatccggttt
    53821 atgctattct agcggcagtt ggtctcggaa tactaattgt cacttttacc aataccgatt
    53881 tcgcgattta tattttaatc ttttcgatgc tattatcacc ggaaattggc tcgcgtacaa
    53941 cttctggtga agggatgact atcagaattg acgacctgat tctggcaatc atcattctca
    54001 cctggttagc aaaaactgcc attaataaag aactgggtct ttttcgcaaa acacccctaa
    54061 ataaaccaat ctggatttat actttagtgt gtatcctttc aacaggaatc gggatgatgg
    54121 ctggaagagt gaatttcctt accggtcttc tcttcgtttt aaaatatatc cagtatttta
    54181 tgatttattt tatggtgatg aatcaaattc gcaccaaaaa acaaatcaat aattacctga
    54241 tttctctgat aatcaccttt accatagtta caattttcgc tttgatccaa gccccgagcg
    54301 gccagcggat taccgctccc ttcgaaggtg aggcaggtga accgaatact ctgggcggtt
    54361 atctcgtttt actaatagct attaacctag gtttagtgtt gaatgacgac gctatagcca
    54421 ataggcatct ccgtcgtggg cttcttttga taacaatgat atcgatttat cctctattaa
    54481 tgacttattc gcggggctcc tggcttgccg ctctagctgt tatcatcgct tatattgcga
    54541 tcagtaaaaa gcgctggatt atttttggag ttgtggtttt agtattcgta ttagctcctt
    54601 atatttttcc ccagtctgta attgaccgcg ttaaatatac attcaccgaa caacagggat
    54661 acgacgctac tctccaggaa catatcggcg gtttaacgat tgatacttcc gcgtccgagc
    54721 gaattcgcag ctggcgggcg gcttttagag atattaccaa acgtccggtg ctcggttttg
    54781 ggattaccgg ctggcgtttt ctggatgctc aatatattcg tgtactcatt gaaacaggaa
    54841 taatcggttt agcggctttt gtctatttgc agttttcact attatctaat attcgtaaag
    54901 tctattccga tgccaagact ccgctatacc gagcgttagc ccaaggcttt tttgtcggta
    54961 tgattggaat gatggctcat agcatcactg ccaatacttt catcatcgtg cgtataatcg
    55021 aacccttctg gttggcgtgt ggtctggtta tggcagttcc cgaagtggaa gaattagaag
    55081 cagaaaaatt accggaaatc gaaaaaagtc ctacaaccat aaatttggaa aatagatagt
    55141 tatcatggga aaaattgact tggattttaa gcctaccacc cgaccagatt taccatttcg
    55201 ggaattaaaa gacggcggag ttgtgtatga atatcgtacc gatactctcc attcactgaa
    55261 tgcaactgcg gcttacatct gggtcctatg cgatggacaa cataccgttg ctgaaataat
    55321 cagttcaata aagcaaaatt ttaatcggtt tgaagttgat cctaccggtg aagtgatcaa
    55381 aattatcgaa aaatttagga ctcttaattt attaactgat gactgatgcc attttttaaa
    55441 attcaggttg gctctgctga gattaatctc gaatgttgcc aatcaatcat tagtgaagtt
    55501 accgcttctg ttttttcggc aatcctaaca ccttcccaaa tttccgctaa aatccactat
    55561 cggattgaga ccaagaacag caaatatttt ctctttcgca ataatcgcct aacccaccgt
    55621 aaccgccaat tatctcgaat tatctatgct ctggaatggc aaatagttca agaccttctc
    55681 actaaaaata aatccgtcct taaatttcat ggcgcggctc tcaagcacct tgataccggc
    55741 tttatctttt taggtgcggc ttccagcggc aagaccagcc ttgcgatcct gcttatgcgc
    55801 tatggctggg agttcttttc ggatgaattc gcccttgttg attgcgaccg gcaaataatc
    55861 ccttttcccc gtaatttgat tatcaagccg catttgcgtg ataaaattaa tatcccactc
    55921 gattacccta ttttcaaact cgacgatgaa tccgacggaa aaattgacgc ccactatatc
    55981 tcgccgtctc ttttcggaac agtttattta agtaaaattc cattaaaggg actagtcttt
    56041 ctggaaaaag ccgaccgcgc tggttttgac ctgcaaccgc tggagcatca caaggctttt
    56101 cggctcttaa cccaacatct tctcaatcgc aactttagcg gtgaaaaatg gctcgacttt
    56161 ttggtaaatt tactgggttc aagtgccgtt tatcgcctga aaatttccag cccgatggat
    56221 ttgacaccgt ccgatagccg agcacttgtt gaacaattaa accaaattga atgaaatgat
    56281 caagtatttt cgcgatttac tcagtgaccg gattcgcatc gaaaatttcc ggcaagctat
    56341 agaatccact gtaaatcata attctattgt agtagaaatt ggaagtgctc tcggtactta
    56401 ttcatttttt gccgcaaaag ccggtgcaaa agccgtatac gccatcgaaa tgaatgacat
    56461 tttctatgtt ggtcgcgaac tcgctgaacg caacaattta actgatcgca taaaatttat
    56521 tcacggccga tcaactgaaa tagatttgcc ggaaaaagcc gatctgatta ttatagagga
    56581 tttcggacca tttttttact atcacaatct cctcgaagtc atacgcgatg cccgagagcg
    56641 ttttttgaaa gcagacggtc gtttcctacc tgcccgaatc actttataca tcgctccttt
    56701 tgaaagccaa gcctggcgcg acgagcttga cctttttgcc gcagataatg accaactgtt
    56761 tggtttcgat tggagttata ccaccgaaat agcctttaat cagacttatt atgccgattc
    56821 tcatcccaaa aagcttctat ccgcaccctt tctcctcaaa acaatagatt tgaaaaacga
    56881 aactaatttc ccattccaaa aacaattcga aataaatatt aaggaaaatg gtcaaattca
    56941 cgggctaatc ggctggtggg attgctggtt caccgaccaa caatttttct ctaattctcc
    57001 ccaatccggc aataattctt ggggtcagct gatatttccc ttccgttatc ctattaatgt
    57061 caaatccggc gagtcggtta aaatttccct gatggtactg gaatcatcac cgagcggttt
    57121 aatagattac aaatggaaaa tcacgcatca ttccggtagt caagaacaag atactttcag
    57181 cggtcggttt tttgatctcc aacaattcaa ccaaatgcgg cgcgatgcta ctcccagttt
    57241 gaacgaaaaa agactgattc aaacctttat cctgaatcaa attgacggaa aacgtagttg
    57301 ggacgagatt gctgagatcg ttatgaaaat gtttcctgaa aaattcgcca gcaaagaaga
    57361 cgtttttaaa atagttctgc cattaagcaa ttttttaaaa ggaaactgaa aactaacgat
    57421 tgaagttttt tagtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    57481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccatta
    57541 agcaattttt taaaaggaaa ctgaaaacta acgattgaag ttttttagtg actttttcac
    57601 aaaagatact gatattaatg gcgataaaat ctccgtcact taaatgtagt tgacggggct
    57661 gtgccattaa accggggagg tcgaaatgaa cttgataatt cagattccat gctataacga
    57721 agaagacacg attgaaaaga ccctcaatga tctcccccga tccattcccg ggattgactc
    57781 aatcgaaatt ctgattattg acgacggctg tcaagatcgc acggtcgaaa ttgctcgcca
    57841 gtggggagta aaccatttcg ttcattttaa tcagaacaaa ggactcgcgc gggctttcga
    57901 agccgggctg gaaaaagcac tggaactcgg cgccgacatc atcgtcaaca ccgacgctga
    57961 taaccagtac aaaggttcgg acatcgcgcg gcttgttcag ccgattctcg ctaaaaaagc
    58021 cgagatcgtg atcggcgacc gccagattga caaaattgag gatttttcct ggcttaaaaa
    58081 aaagctcgag aaattcggta gttacgtagt ccgcaaagtc tcccaaacta atattccaga
    58141 cgtaactagt ggttttcgcg cctatagccg tgaagccgca ttgcacataa atatcatctc
    58201 tactttctca tatacgctgg aaaccattat cgaagcagga caaaaagaca tcccgataat
    58261 tagtgtacct atcgaaacca ataaaaccga acgcccctcg cgcctgttca aaagtaattg
    58321 gtactatatt cggcgttcct ttgcaacaat tgtaagaatc tacactatgt accagccttt
    58381 aagagtcttt ttctatactg gaatcacgag tttcggcatt ggtctaattc tatctatccg
    58441 ttatttaatt tttatgattg ttggccaggg caaaggccat attcagtcgg taatcatcgc
    58501 cgccgtcttt ctcattatcg gttttttctt gctaatgatt ggcttgctag ctgatgtcat
    58561 cgccgcgaat cgcaaattgc aggaagaaac tctttaccgc attaagaaag attcactgaa
    58621 gtaatttgaa taataatact taatcaacac tatataagct aatcatctcg tcagaaactc
    58681 gacattataa atgaaaaatc aggaagcaga aggcttactt tcccccctta tcaagaaaat
    58741 tcgtctgaaa aaggcagcaa aatgtattca aaataactcc acagttcttg acctagcttg
    58801 tggcgccgga tatttgcgcc aatttctacc aagtcaatgt aaatattatg gcattgatcg
    58861 aataacacct ccttcattaa atctttttaa tgccttcctc caacttgata ttcttcagcc
    58921 agaatcgttt gaaactataa aggaatggct tcctgaacct aaagttgatg ttgtgactat
    58981 gctagctttc atcgaacata ttaaaaatcc ccaatattta ctgaaacaaa gtctattgtt
    59041 attaaaacca aatggtttat tgattattac gactcctcat ccaataggga gaaaattgca
    59101 cgattcgctt gccaaattat atctttgcag tctttcaggc gctaaggaac atgaaaattt
    59161 cttcggacaa caagatctta aatttctgct aaaacagtct ggatataaac tgctttctta
    59221 tgaaagattt ctaatggggc ttaaccaatt gattattgcg actccattaa aataagttta
    59281 tcataattat cctcacatta attacaatga aatatttttc acgcatacaa aatctactcg
    59341 ataaatatga aatcccaatt atatttgcga tccttttaat tggtttggtt atccggctgg
    59401 cggtcattat cacaattgaa gaacctatag accgagatgc tttagaatat tataccattg
    59461 ccaaaaattt gaacgaacac catcgttttt cgatcgacgg cgagaaccct accattagac
    59521 gctcgcccgg ctaccctttc tttttagcat tttttatgaa aattgtcgga actaatccga
    59581 accatttata cacagttcaa gcacttatca atatattgac aatctggatt gtatatttag
    59641 cactaaagca aataggtgcc aatgcgacaa tccggatcat tgccatttta ctttttactt
    59701 ttaacccatc attcatttat gttaatacat tttatgcaga aatattgaca atgtttatta
    59761 tgtcattatt aatctattta actataaatc aagaactgtt caaaaacaaa aactggaata
    59821 tcatattaca gggcatatta attggggtat tggttctaat ccggccaacc tttctatatc
    59881 ttccaatttt tattctcgta gttagtatat tgatccgcat tttctttcat aataaaaaaa
    59941 tgaaagatgc gctgattacc gcaattatcg caattcttac cctggtgccc tggactattc
    60001 gtaatcggat tatcttcaat gagtggattc caaccgaaag ttccagcgga ttagcactct
    60061 ggcaagctaa cctcgaaatc gaaaaccgaa ccgtatggta ctctgttacc gacatcgcta
    60121 aatatgaaca gcaacgtaca gcatcagccg aattacagtc aaaactacgt tcggaatact
    60181 tacaaaaata cgatattaat tctttagttg aattaaatca gtttctggaa caaaaagcca
    60241 ttgaaattat acttagacat cctatcaggt attgcatttt atgtgttaac cgatttttaa
    60301 tcttctggtt ttcgccaccc atcggctcga cgactttaaa agcaatatca ccagtaattt
    60361 tcgtacttgc tttattattt agatacctta taacgattct cggcatttgc ggattatggc
    60421 aatttgctcg gcgcgatttc tctggctctt ttattttgat ctctctagtc gtttacctga
    60481 ctgccttgca ttctgcctcc catgcaatac aacgctactt tctaccgctt attccgctga
    60541 tatattttgc ccttggatat tttcttaata accagaaatc attaaaccgc aaagggcgcc
    60601 aagaataaat acttaatatg ttttcttatt taggtaaata tatccatctt tgcgctcttg
    60661 gcggtgaatg gaatccttcc ggagtaaaac gtggctgatt ccgtcatcga gcaggctaag
    60721 tccggcgtcc gttgggtcgg tatcgggcaa gttggttatc gtcttctctc gatagcctcc
    60781 tcaattgttc tagcgcgctt acttatgccg gacgatttcg gtgtcattgc gctggcgcgg
    60841 ttggttctgg gatttgtcat gctgttgagt gcctggggaa tagatgcctc catcattgtc
    60901 gaagaaaagc gacctaaaca gattgccaac gttgctttct ggataaattt tggcattatg
    60961 ctggtacttg gcttattggt tattggcatt tcaccctggg taaaaaaatt ctacgatact
    61021 ccggtgctcc aaccaatcct aatctggatg ggactgggac tgattttcca gtctttcgaa
    61081 ctggtgccgc gaacgattct taatcgtgat ctgaattata aatatttgac aataatcaat
    61141 ctaagtatcg aaattttcac acagttactg gtaattctgc tggcaatttt cggttgtggt
    61201 gtctggagtt tggtcattcc acaaattttg gctagtccgc tcagagccat tccatattgg
    61261 ataaagacca aatggcggcc atcactttat atcgaaaaag ccgatatcaa acaggtatta
    61321 tcttttggca aaagcattct tggagctgag cttatccgct atttgaacac caataccgac
    61381 tacttcctga tagggaagct actttcaaag gacaaactcg ggtattactc gttcgctttt
    61441 aatctcgcca actggccagt tgagaatatt gtaaaggtaa tcaatacggt atcgcttcca
    61501 actttagcca aagtccagtc agatttggct gagatgcgac gcttatttct gcgtatgacc
    61561 gaaatggtct cgctggttac tttccccatt ttcggagttc tggttggtat tacttatgaa
    61621 ctgctcgtcg taattttcgg aacaaaatgg attccggcag tacgaccact tcaagtcatc
    61681 gtaatttatg ggattatgcg ttcgctcttt tcgccggctg gacggatttt cctgattcag
    61741 aagaaaacac actatatgtt tttcatcaat ctcgctcaat ttccatttct ggttgtggca
    61801 gtctggctgg gcattcggaa ggcggaaatt atcggcgcgg cggttggtgt aacaatcgta
    61861 ttgtcaatcg gcggtcttat caccataatt gtcgtatcaa aaatgttgaa gatgaaaatc
    61921 accgaattta ttagaacgat ctcaccgggt tttctctgct gtgtcatctt tctaatctgt
    61981 ggttggtttt tcaaacacct gcttttcgga ttggggttgc cgatggtcct gatcctgctt
    62041 ttctatttat tatttgcagg tagtttgtat gcgctgagtc tgcgtctttt ttacccacgt
    62101 ctttttattg atttcgtaag aacttcaatc agtatcattg gaattacacc gcgtaatgta
    62161 tttgagaaac tgagaggcaa ccagtaatta tgactccagg gactaacatc gtttatctat
    62221 gcgccaatcc cggcct
//
LOCUS       DAOOMU010000003        57504 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_365_length_57504_cov_12.744539, whole genome shotgun sequence.
ACCESSION   DAOOMU010000003 DAOOMU010000000
VERSION     DAOOMU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 57504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57504
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_365_length_57504_cov_12.744539"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /locus_tag="PLI55_00545"
     CDS             <1..1878
                     /locus_tag="PLI55_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPA99358.1"
                     /translation="SYSANVGNAIYIDNFRIVYLDEIKKPVVVEAESGVVGSDFSILE
                     EDSITYVTIQTNLAAYNPGSSARVISYEVTFPDTGSYDLFVRLRTGADTFNDDSFFYG
                     KGFGEKDPSVDSVWVCINGLAAAGFSGPNEIVRDAGGLGSGVWKWVNLSRNVYQGSVE
                     SLVTFIVDDPDSLTQTFQIGARENGLDIDKIAFGKSYLYYTVGNLDNREPGSTDLPGE
                     VWEGPPLAHKQPKFVGNIYSSPQISNFEAYWNQVTPENAGKWGSVEGTRDVMNWSGLD
                     AAYKLAKDNGFPFHFHVLVWGAQQPSWINDLTPEEQLEEIREWFEAVAERYPDIDFLE
                     VVNEPLPGHNPPDGTSGRANYKEALGGNGETGWDWVIKAFEMAREIFPEGTRLMINDF
                     NIISSSSSTSQYLKLIRLLQERNLIDIIGEQGHAFTTTAAVATMKKNLDSLASTGLPI
                     QITELDIDGPSDAVQLQSYQRIFPTLYEHPGVEGITLWGWRRGLWRDEQGAYLINQDG
                     SERPALVWLRTYLDTLNLNVMVKEDIINVLDEFSLGQNYPNPFNPTTTINYQLPTTSH
                     LTIKVYNLLGKEVATLVDDVKQAGSHSVTFDGSGLAGGVYLYQLKTANYTDTKKCLFL
                     K"
     assembly_gap    1879..1978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1979..3007)
                     /locus_tag="PLI55_00550"
     CDS             complement(1979..3007)
                     /locus_tag="PLI55_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPA99359.1"
                     /translation="MTRITIEEVAKQANVSKGTVSAVINGKNSVNPATREHILKVMKD
                     LNFRPRGMARNLKNGDQVRCIGIIIKDLNYPFYTSIAAGAREYANSQGYSVIVASSEN
                     DHECEKKFTHLFSIKDIKGAIIAPIVEGEAEIDHLFKLKRINYPFVLLEDVKGIQANV
                     VAIDNLSAIKTAVKYLIDNGHTKIVHFAGPPQSSHTQERIEGFRHAFSESPLIFNKNL
                     IISIGSRHEEAYGNTMNYFKNRSREDYPTAIVCFNDQQALAVMTALKELNIRIPEDIS
                     IIGNDDIIYAKIYPVPLTTIRAPQREIGYKAAEILIRNIESSTAIPNEKIVLGTDLVI
                     RESSRVLN"
     assembly_gap    3262..3361
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3762..6731
                     /locus_tag="PLI55_00555"
     CDS             3762..6731
                     /locus_tag="PLI55_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 115 family protein"
                     /protein_id="HPA99360.1"
                     /translation="MNQISRNVIRFNCCKSFTSLITHFAKQVTLILLSLLILCPNILE
                     AVTSDMDSYISVEAIGGGFPLSVAGHTAPLYASSQDYPGVLRVLKLFQSDLKMVTGIE
                     PVIAIDTIPKSKEIVIVGTIGKSPIIDALIQSNQLDVSDIAGKWETFLIQRIEKPYPD
                     VEQALVIAGSDKRGTIFGMFDLSEKIGVSPWYWWADVPVKKKDCIYIKPGRHTLGEPK
                     VKYRGFFINDEAPALSNWAYEKFGGFNHEFYVNVFELLLRLKANHLWPGMWGKYFGSD
                     PENPRLADEYGIVMGSSHCEPLLYNNDPGAGLWNSQTMGPWRYDINRENIYKVLDETV
                     AARGQYENIYTVGLRGIHDTQMEGGVDVQEQIALLEQVFKDQREILTKHINKEITEIP
                     QVFIPYKEVQDYYDAGLQVPDDVTIMWSDDNWGNIRRLPRFDDKPRAGGYGIYYHFDY
                     VGDPRNYKWLNTVQISRVWEQMHLAYRYGVDRIWVVNVGDIKPMEFPINFFMDFAWNP
                     EAWPAERLPEYTRLWAEEQFGAQYAEEIAHILTQYTRFNARRKPELLSPETYSLVNYR
                     EAETVVAEYNDLAKKAREIYEALPTEFHDAFYQLVLHPTEACANLNELYVTVARNRLY
                     AHQKRALTNELAQRARDLFKKDAEITDRYHNIANGKWNHMMDQTHIGYTYWQQPEENT
                     MPEVIEINVPESAEMGVAIEGSTECWPPANSEAILPEFDPYNQSCHYIEIFNRGKKPF
                     KYTIKSGNPCVLISQKKGEITTEERLWIEIDWKQAPKGKSTIPLIIKGPRKSQVEVKI
                     IIFNPAVPVEELADRFLESNRYVSIEAEHFSNAVNTRGVYWQIIPDLGRTLSSVTPFP
                     VTMSSLIPEGDSPRLEYQVYLFNAGEVKVKAYLAPTQNFPNGPGLRYGISFDDEPVQI
                     INMHAGKTHRDWQNSVSDYITIMTSVHALKSPGKHTLKFWAVDPGVVLQKLVIETGEV
                     KPSYLGPPESRWFKIAE"
     gene            6763..8445
                     /locus_tag="PLI55_00560"
     CDS             6763..8445
                     /locus_tag="PLI55_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HPA99361.1"
                     /translation="MKKVIITMTLIVASFISADAFSADQKAFYYNPILAGFYPDPSIC
                     RVGDDYYLVNSTFAYFPGIPVFHSRDLVNWKLIGHVMDRVEQMNLEGLGVSEGIFAPA
                     IRFHDGLFYVTCTLVGAGGNYVVTAQNPAGPWSNPVWLPQIDGIDPSLFFDQDGKAYI
                     VYNSVAPNNRPLYEGHRTIRMYQFDYEKLKVISPEIILVNGGTDISKKPIWIEGPHIF
                     RKDNFYYLICAEGGTSWEHSEVVFRSEQVTGPYVPYAKNPILTQRHLDPNRPHPITCT
                     GHADLVMTPAGDWWAVFLGCRPYLPEYEGFFNTGRETFMAPVKWIDGWPVIDPDHNEI
                     QYSYPLPIILEKPIKADIPYSGNFRIRDDFDSEKLDLNWVFLRTPHTKWYSLSDRRGS
                     LAIQLRPETCSGKSNPSFIGRRQQHLRGSASTAMDFVPVSEFEKAGLLVFQNEKHYYF
                     LGKSIEKGEPVVQLYKTDTLLASHKIGEGRNIRLKVVADGAEYSFYYATKPEEWILLK
                     DKVDARFLSTRVAGGFVGCMYAMYATSSGEASSNVAYFDWFEYVGDDEIYHQ"
     gene            8465..9502
                     /locus_tag="PLI55_00565"
     CDS             8465..9502
                     /locus_tag="PLI55_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPA99362.1"
                     /translation="MTDQQWETLKKVINGEKIEPLPVGFIADSPWLPGWYGIKILDYF
                     SNDELWLKANLKAIQEFPEVMFLPGFWSEFGMCTEPSAFGARCRFPQNEFPHAHPVIK
                     SPEEINALVVPEPETDGLLPFVLNRLKLAQPYIENAGHKIRFAIARGPLNIASFLMGT
                     TEFLMAMLTSPELVETLMEKITAFLKNWLRLQRSTFSTIDGILLLDDIIGFIGEQEFC
                     RFGLPYFKELFSADVSVKFLHNDAQCRVSVPYLPEIGVNLFNMGFDVSLNDIKTWTNN
                     QVTLLGNIPPRDVLANGKPEDVVKSVTELLDSLEDQTRVILSCGGGLPPGVSTENLKA
                     FIETVKNYTLK"
     gene            9571..10845
                     /locus_tag="PLI55_00570"
     CDS             9571..10845
                     /locus_tag="PLI55_00570"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPA99363.1"
                     /translation="MNKRSNILFLNWHPPFLLGIVIAYFAISVRFLPAAEKVDSEKVI
                     RKIADNVLKDANFNFIDQESGKRYSSTADAPSGARLVPESRYNDWRYWNGVLNIAMLR
                     LGEVLKEPTYTEFAVRNVAFSFDNYAYFEKRYANENKWEYPFGQFFMMEELDDCGAMG
                     ASVIEIMRLDPQERYLKYIDRAATHILTRQSRLEDGTLVRTFPRKYTLWADDLYMANS
                     FLCRMGKVTERSWQTVGIDDATRQVINFHKYLFDQEMGLMYHNWYSDTGSPGVAFWGR
                     ANGWAILAQVELLDQLPADYPQRDTLLALFRCHIDGIVPYQDSTGLWHQLLDKPDSFP
                     ETSCTAMFTYAIARAVNQGYLESRYKSFALKGWQGILTKIRPDGQVEGICTGTSTSDD
                     LSDYYTRPTPLNDGHGIGTVLMAGTEIIQLLK"
     gene            11135..12475
                     /gene="xylA"
                     /locus_tag="PLI55_00575"
     CDS             11135..12475
                     /gene="xylA"
                     /locus_tag="PLI55_00575"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HPA99364.1"
                     /translation="MSKQVLIGKREYFKGIGKIPYEGPESKNPLAFKYYDENRVVAGK
                     KMKDHFRFAIAYWHTFCNNGGDPFGAEGTVDFPWNESKDPMQAAKDKLDAAFEFFTKL
                     GTPFYCFHDRDMAPEGKNVVESEKNLKELVKIAKAKQEESGVKLLWGTANVFGHKRYT
                     NGAATNPNFYVIPHVAAQVKAAIDATIALGGENYVFWGGREGYYSLLNTNMKREIEHM
                     AMFLTKARDYGRASGFKGTFLIEPKPMEPTKHQYDFDSATVIGFLNKYGLQNDFKINI
                     ENNHATLAGHTFAHEVQTACDAGLFGSLDINQGDPFNGWDTDEFLHNLYDATELMLIL
                     LQNGGIGHGGMNFDAKNRRSSTDLEDIFIGHIYSMDTLARGLLIANDILEKSDYLKMK
                     KERYSSFDSGDGARFEKGELGLVELAKIAEEIGEPKMISGKQELYESLINQYIR"
     gene            12499..14085
                     /locus_tag="PLI55_00580"
     CDS             12499..14085
                     /locus_tag="PLI55_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HPA99365.1"
                     /translation="MFKLFAGLDVSTQSTKLVIIDLAAKKVVYVNSVNYDTDLPHYNT
                     KNGAIQGLGEGVSESDPRMWIEAVDRVFANAVAAGIDMSQIGCIAVSGQQHGLVALDA
                     QGNLSRPTSKLWNDFSTLEECEILTEKVGGLEQMIAEVGNSQRTGYTAAKIFHMVRHE
                     PEIYHKTATLFLVHNYINYYLTGGVRVMEPGDTSGMALWNPKNGQWSQKVIQAIAPDL
                     MSKLPEVKPSDQPIGYISSELVAKFGFSKDCLIDAGCGDNMYGAVGTGNVEPGIVTVS
                     LGTSGTAYTFMEEPYIDPTGEIAAFCDSTGHYLPLLCVSNLANGYNEFIKLHKMTHQV
                     FDKMLDETPPANNGKLLIPWYVGERTPDLPLAAPIYFGFGLADFTQANLARAIIEGHI
                     LNLYTGFKRMPVDVKEIRLTGGLSRSDTWCQTIADIFEAQTVPVEGEGAALGAALHAA
                     WTWSKAQNKDISLKELVKPFVVLDEKRRKQPIEANLPVYRKLSAMFESISCRIRGLTG
                     DDPFRLRAELIKLSKTKANN"
     assembly_gap    14177..14276
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14277..15289
                     /locus_tag="PLI55_00585"
     CDS             <14277..15289
                     /locus_tag="PLI55_00585"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPA99366.1"
                     /translation="GAKMKKYKISNDKGVSAEIISYGAIVVSLTAPNRNGQLEDVILG
                     FDDLASYEKDNTFQGSIVGRYGNRIAGGKFSLDGKVYQLNLNDGPNHLHGGPKGFFKV
                     IWDIEQLDSQSVRLTYVSPDGEEGYPGEVKISVVYTITDDDALRIDYYGTTDRPTILN
                     PTSHCYFNLSGSPKNTILDHELMLNADFFTPIDEFSIPTGEIRPVAGTPLDFREPKPI
                     GRDINAEDEQIKFGKGYDHNWVINGKPGQVRLAATVYESKSGRLMECLTDQAGLQFYS
                     GNFLDGSLIGKGGIRYNYRCALCLEAQAFPDSPNKPHFPSVVLRPGEEYRQTTIYRFS
                     VR"
     gene            15769..16821
                     /locus_tag="PLI55_00590"
     CDS             15769..16821
                     /locus_tag="PLI55_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPA99367.1"
                     /translation="MTIKDIARLANCSLSTVSKALNGRQDVSEATRQRIMQIVAEYNF
                     TPSAYGKGLKKQKTENIGVIFCRETRPLSANPFYSRVLEGIEAELVINNYNLVLHLLP
                     EVYRGEYPKMLKEHTVDGIILAGVLDQDFVDLVKESGIPIVFVNPNFEIEGEDQIFID
                     DEHGAFQAIQYVISKGHQRIGFVSGDLNRISFKKRYDGYKKALAYHRIEYDDEIVRTG
                     GLENGYDQVTALMKLKNKPTVIFCTNDLNAIFGYKAIEDLGYRIPQDISIIGFDDIDL
                     AKISNPPLTTVRVYKEEMGSIAVRILLEKINKTEHKPVTTLLPVRIIERDSVKDLNNL
                     KNTVRHIEPKQDVELV"
     gene            16818..19757
                     /locus_tag="PLI55_00595"
     CDS             16818..19757
                     /locus_tag="PLI55_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA99368.1"
                     /translation="MKRYLKPAFSALAVMLFINLLNAGVIRGRVVDSKTGDPLIGANL
                     ILAETAGIGAASDFKGYYRIDNVPAGEYTLKVTFIGYQPVEIKVSLKADETIVRDIEM
                     VYGGALKGEEVVVTAQAKGQMSAINQQLASNVITNIVDESRIQELPDVNAAESIGRLP
                     GVSIQRSGGEANKVEVRGLNPKYSLITVNGVELPATGSEDRSVNLSLISSNMLDGIVL
                     KKTNTPDMDADVLGGTIDLRLKEAPEGFKFNLSAQGGYNQLQKYYGNYNFTASVSNRF
                     FDNRLGIIVNANTDNYDRSADKLRDNWRNYNEMLATGELFMIEEIVNRKRTGASLLLD
                     YNLPKGKITANGFFNQLNSDGLIRNSQVNTMELGYATNRHYYRVEEEKSKTNVFTSAI
                     GINQDFGWFRYDAGVSYSGTNTDSPDRRVTEFVQEQSGLLPGYPKFVDPKALVPYFNV
                     DTINTNMASLYSYSKKITENTSSAKLNLEIPFRFGNILSGSFKTGGKLRWIDRSNDEN
                     QHGYSGLQYGSGQQNPVFLHVDKVYPEWGIMDNVEEYQLLNVLPFLKDYSRPDFLNGD
                     FPLGLTFDEKKLHKMSDALMAAPDSLNLWLPYSVGTFGYDYDGFERYQAAYFMGEFNF
                     GSYLTLIPGVRYEKDYSKYHGQRYMVNQSGQTVEQPPSEFVRLTKERENEFWLPMVNI
                     IAKPTNWLNIRLARTETIARPDFLRYAPISYISSDRKSVNAANYSLRPSQSTNYDVGV
                     SLFNNEIGLLSVTGFHKNIEDLIFYSAAFYRPGVVLDSALDIPQSWLKANPQINAYRN
                     NPDPAKYYGYEIEWQTHFWYLPSVFKGIVLNINYTHIYSEMKLVYDSLVTQQVGSRRV
                     YSLVPAYIKTRMPDQPAHVFNITIGYDLKGFSARLSYLFQTDKLTGIGYDGVIPTTRL
                     SSYTSDYGRWDLTLQQKLFQNIQLFANFNNLNNRYDQSCIGADLTHPSYIEYYGFTMD
                     LGIRVNL"
     gene            19956..22559
                     /locus_tag="PLI55_00600"
     CDS             19956..22559
                     /locus_tag="PLI55_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPA99369.1"
                     /translation="MKSKNLVVWFTMLAVVLIMSAGQILAQTGDTLVVNWDDGAGNIV
                     QDALYNAVIGDTVAGGARANLNRVYKLKAGGYYWNYKTISNDFPLRLVGEVPTGDEHP
                     AVIQMVTDAESGSTPGKMISCASDLTLKNLYIIGSDELGSSGYYQPIEVNAVAKRFII
                     DNCVFERSNFAIVAFAGGKDNDLFITNCVYRNLQEDVPTQQWCGRGLSVWTDIDTVVI
                     ENNTFFNVNMCAIQIENGAANYLRFNHNTLVNIGRAFTSTTNVWWREAYFTNLLFVNT
                     WWHGEGYCDYSVQYQPGRDPRAYTTGIFTINDMPSRYGTNKGRRILFSNTAAYLDPYF
                     TEHYGDTIRFQPYVNAITDSFFTTFSPANGGQMVIKDTTWLSTKPAFTNDPNTPAQLE
                     AMWNHITASRGYQYYGTGVQAKPYYYALPTDPVTGDTLWAEPSWPLPENFTYSNDLTT
                     QSTDGLPLGDLRWFPAAKATFEANKDSYVNAIEWMPGERIIIKPIWEEQAEAGTVSGT
                     AAVEPFTGEAWYTLQGGTSVEWTFNSTYAGHIDMKMKARADGQNIGFDFILNGKNIVD
                     AARKWGQFVVWTGSGDPSTFWTGKSTTEFYEAFYAFADLHSFGSLDEIGVVAGENTLK
                     LQYSWNPISFQWIELYEAGTTNLIARLVPSAAVNNGATPGGIGDWVPQGFNSVALGTN
                     GTVTFSLPNLDAGRYQVRVFYQNAGAASTGKVLVDGAQATTFSFASQPAGTGLDVITD
                     LFDLTAGTHTLAISGSGAKVDWIQLLNESRISGIKKVTVLDGFSLSQNYPNPFNPTTM
                     IRFELGKPSNVRLTVYNLLGQRVATLVNGHMSQGAHIIQFDASHLASGIYFYAIQAGE
                     FKEVRRMLLLK"
     gene            22661..23764
                     /locus_tag="PLI55_00605"
     CDS             22661..23764
                     /locus_tag="PLI55_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPA99370.1"
                     /translation="MNRREFNKMLLCAVGSLALPKIDLFGSKLEQAPPLHFPRYRGFN
                     LLAKFSNWEPRSKFDEEDFEIMAELGFDFARIPMSYWCWSSKDDWYRIDEDVLKDIDE
                     VIEFGKQYNIHINLNLHRIPGYCINGRDQEPMDLYEDTPERMQKALDAAIFHWKMFAK
                     RYKGIPNSQLSFDLINEPPHTKEETRHVEVVTALVNGIREEDPHRLIVADGKDVGRTP
                     IFGIADLGVVQSTRGYDPMSVSHYKANWVPKESYQTFKKPTWPLKGDDGTIWDKAMLK
                     SQLIDPWMPLVAKGVPVHVGEWGCYNQTPHDVALRWMRDLLSLWQEVGWGYALWNLKG
                     DFGILDSKRKDVKYEKYKGHDLDRKMLELLKEF"
     assembly_gap    24147..24246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24247..25524
                     /locus_tag="PLI55_00610"
     CDS             <24247..25524
                     /locus_tag="PLI55_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99371.1"
                     /translation="EQAAEASQKLIIDPAKKVNPKVKIIIKYPNWYDHFQYCGFNLEK
                     QPTQFDLIYTGTETRDPVYTHQHLQAYESYAIMRYFENIAPGRNGGGWVDPFNRRTLD
                     RYAEQINMTLFAKARELTLFCFGALLEAIPQEDGSFRPLSPVAPVAGYNLDKIDCFLG
                     KLGQPFGIKTYKPYHSSGEDFLPNYIGMLGIPMEITPDFPANDEIIFLTEQASFDAKI
                     VEKIKNQLIKGKDVVITSGLYRALQGKGIEDIVELECTDKKALVHRFYDWQNVFIAEN
                     DILIPQIRYATNDSWELITALDSGNGYPIFHQASYANGKLYVLTIPDNFSELYELPRE
                     VLTIIKRVMMKNFFVYTDSPSSVSLLVYDNNTFIIYSFKEHNTDVNIVLDNKFSKVTD
                     LLANRELTGYKENNKLVIKTAIGPHSYRVFRAE"
     gene            25561..26583
                     /locus_tag="PLI55_00615"
     CDS             25561..26583
                     /locus_tag="PLI55_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HPA99372.1"
                     /translation="MMRIPYIFGITVLLIFMVSCQSGSKAVDAFEQNEKLGRGVNILG
                     YDPIWDSFEKARFKEHHFKLIREAGFNTVRINLHPFRHMDPTTFELREHWWKVLDWAV
                     ENALANGLMIILDMHEYYAMADDPVARKDMWLAFWKQVAERYQNSSENVLFELLNEPN
                     GKLNSELWNGFLLEGLAVVRNSNPHRTVIIGPAQYNQINQLDSLILPEKDRNIIVTIH
                     YYSPMAFTHQGASWAGRTDSLGVQWLGTAEEKQAILDDFAKAQNWSKKHNRPLFLGEF
                     GVYDKADMESRTKYLSFVTSTVEDLGWSWAYWQFDSDFILYDIDNEHWIEPVLKALIP
                     KTAGVN"
     gene            26709..28010
                     /locus_tag="PLI55_00620"
     CDS             26709..28010
                     /locus_tag="PLI55_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015331077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPA99373.1"
                     /translation="MNSDKEMLLKELEFALKNEFLDVWYPAALDTVYGGFLSDFTFDW
                     RPEGPQNKMIVTQSRQVWTASQAAMFYQDDQYRKIAEHGFNFLRDKMWDKTYGGFYQL
                     RDRQGNLIPYGDGLHKVAYGNAFAIYALATYYEAFGDTSALDLARQTFAWLEKHNHDP
                     QYKGYIDATNLDGSWIMWHPTGKTNRNITETVLKDYNSSIHLLEAFTQLYKVWPDSLV
                     RERLLEMLTLVRDTFVNKKGYLNMYFTRDWQPLSVYDFSAEPQEADYFFDHVSFGHDV
                     ETAYLLLEAEHILGKKSYSVTLPVARALVDHALENGWDQDNGGLYYQGYYFDGSDTIT
                     IFDDTKEWWVQAEALNAFLLMAKLFPQDQKYYDAFRKQWEYIQRYLIDHQHGGWYSKG
                     LDKSPERAKGPKGYDWKVNYHETRALINCIQMLKDEHELLK"
     gene            28029..28859
                     /locus_tag="PLI55_00625"
     CDS             28029..28859
                     /locus_tag="PLI55_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HPA99374.1"
                     /translation="MKKLFRFYIVLIIIVSLAFSQEKNKVDLSTGANFIATKTYAPEE
                     DARILKLFEGLRVADVSDGMDMVGLPGVGLVDPAIHADWLDLEDLSHQIRGIAVTARY
                     VPTQRPDRPQAGEKFQEWEGNFYNVYSPETFAELIRPGSVIVLDDVEEKDIGTIGSYN
                     ILAWHKSGAVGVVTDACARDTDEIAKEKVPLYLRKKGRGIRPGRNELESVNRPVVIGG
                     VLVCPGDVVVADGDGVIVVPRNVAEQVARYAREILVKDKASRKDLYKSLGRPLDKTVE
                     "
     gene            28913..30736
                     /locus_tag="PLI55_00630"
     CDS             28913..30736
                     /locus_tag="PLI55_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HPA99375.1"
                     /translation="MRLKLIDIVIILLYLSFTVVIGLVLKKRAQENKTAYLLGGNKLP
                     WYMLGISNASGMFDISGTMWLVTLMFVYGLKSIWIPWLWPVFNQIFLMVYLSAWLRRS
                     NVTTGAEWIGTRFGSGRDATLSHNIVVVFALLSCLGFLAYGFIGLGKFMEIFIPWEVV
                     SNYVPFSVPDKFVPHLYGIVFTAFSVFYTILGGMVSIVWADVIQYCIMGVSAIVIGII
                     AMRALSIHPLIVPEGWMSPFFGWELNMDWSGIIAGVNDKIASDGYSLFMIFFMMMLFK
                     GILVSAAGPAPNYDMQKILATRSPKEAGMMSGFVSVILNPIRYIMITGFAVLGIIYYD
                     RLDLIVGGRLDFEQILPSAISEFVPIGFTGLMLAGLLAAFIGTFAGTLNAAQAYIVND
                     IYLKYMRPKATNRQVSSVTYISGIGIVVISIIFGMFALDINSILQWIVSALWGSYVVS
                     NVLKWYWWRFNGYGYFWGMVAGIIPALIFPQIFDTLDLYYFPLILLISLAGALIGTYR
                     HPPTDEAVLKKFYKTVRPWGFWKPIHEKVVAEDPNFVNRSSWKRDLLNVLIGIIWQTT
                     LVALPIYFVLKKFGNAGICLLIIIVTSIFLKKNWYDKMEDD"
     gene            30777..31997
                     /locus_tag="PLI55_00635"
     CDS             30777..31997
                     /locus_tag="PLI55_00635"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HPA99376.1"
                     /translation="MKNDAIANWRQQVSRNLYENIIPFWMTYSIDRRNGGFYGRITND
                     LQVDKTAPRSVVLIARILWTFSALFRFDKDTRHLEMAHRAYNYLTKYFSDSKYGGVYW
                     LVAPGGEVLEDKKKIYGQAFAIYGLSEYYAASGEKAALEMAHQIFQLIEKYCHDAING
                     GYYEAANRDWSETQDLRLSAVDLNEKKSMNSHLHLLEALTNLLRVSSDSLVRARLQEL
                     VECHLRYILNPVSNHLYLFFDEKWNPRLNGVSFGHDIEASWLITESAEVLGDDKIQAR
                     VKSAVHKIVAAVIAEGFNQHNALPVANDITGSLSVNNFEWWQQAEALVGLINAYQLYG
                     DSSYLDRACRLWDFIDKYFVDWQRGEWYYEISAEGIPNPAKPKVSEWKCCYHNTRACL
                     EVLKRTAQLINRRE"
     gene            32077..33204
                     /locus_tag="PLI55_00640"
     CDS             32077..33204
                     /locus_tag="PLI55_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HPA99377.1"
                     /translation="MPNQACPSQNGIYQRYQHPVLTAKHIPINWRFDLNYATNPFLME
                     RLGVNAVFNCGAIKFNDKYCLVARVEGLDRKSFFAVAESPNGVDHFKFWEYPIIMPET
                     EIPDTNVYDMRLTHHADGWIYGVFCTERKDPNVPEYDTSTAVAQAGIARTHDLKKWER
                     LPDLQTKSRQQRNVVLHPEFFDGKYAFYTRPQDDFILAGTGGGIAWGLADNIEKAIID
                     EEIIIDSRVYHTINEVKNGEGPAPLKTAEGWIHLAHGVRNTAAGLRYVLYMFLTDLNE
                     PWRIIHKPGGYFLAPEGEERIGDVSNVVFSNGWIADPNGRIFIYYGSSDTRTHVVMTT
                     IERLLDYVKNTPEDGLRSAKCVEQRVALINHNWNIIDNFKK"
     gene            33259..>34105
                     /locus_tag="PLI55_00645"
     CDS             33259..>34105
                     /locus_tag="PLI55_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99378.1"
                     /translation="MKKSILIIMLSTISLFGQIPGFTDDFEDGSLDTLWNDSLMTLWH
                     SDWYPDIFNLTESEGVLEVAYDRKSTDSDWPAFYFTPPEVINVAHNPKIVIEIKSDIT
                     INFAIKPVYTNGKDNYQSTTVPGSNTWQTYTFTLSNYAGGYCNQVVFHFDGGTKVDKQ
                     GTVCFDNFIIAGFSISIQGLNAKFDPINQYVDLVWNCDNEAAVDHYNVYRDTLKGFSC
                     TTENLIGEPEGKNWRDTQIGIGTYYYKVAAVDTMGKQHAVSMEMACRTYTIGAIPVVE
                     IADVNS"
     assembly_gap    34106..34205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <34206..>35567
                     /locus_tag="PLI55_00650"
     CDS             <34206..>35567
                     /locus_tag="PLI55_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99379.1"
                     /translation="ESDYHGCLNVSSVNPHYLIYHDGTPFYGIGAYYPWGVRNDDDGL
                     GILAASGGNLFGYWNGNYDGAGNGGGIYQIESIQSGIGYYDQRKCARIDEILDWAEQR
                     NLEMMLAIWPHDILDQSTWGYKGWDDNAYKTVCNAANFYTDSLAWVYQQKLYRYMLAR
                     WGYSRSLGIWELVNEIDGTDGWSRGNKTGALEWVQKVHNYLKENDYFKRPTTISKSGS
                     SANYWPEGYQICDLPNVHLYEKGWSAPYSSDPIRSSYWTYRNVARQLWTGFEKPGIFG
                     EAGAEPTNMYAANIEKGSSKYVEIYHNALWSAWASGLAATPIWWEMNDRELMTDEVFA
                     QMKAFARVASDIDYADLNLISSNASADTADAFMMTADSLGFGWFRSYNNSAISGRFTR
                     ISGLKTGTYQAIWYDTWAGDSVIVGNCVSAGNMIYTEIPEMPTPCKDIALRLTKTEDG
                     TQPA"
     assembly_gap    35568..35667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <35668..36012
                     /locus_tag="PLI55_00655"
     CDS             <35668..36012
                     /locus_tag="PLI55_00655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPA99380.1"
                     /translation="STEGLASVSLFNNVLSEIEVEKNASIPTEFALKANYPNPFNAST
                     VIEFSLPKTTNIKIDVYNLTGQLVETLVRGRQSAGNYKIFWHPANLSSGIYLYQITCP
                     EFTATRKCMILK"
     gene            36204..>37204
                     /locus_tag="PLI55_00660"
     CDS             36204..>37204
                     /locus_tag="PLI55_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPA99381.1"
                     /translation="MSTIKDISKKAGVSISTVSLVLNNKGYVKPETYQRVLSVMHELN
                     YKPSRSARKLATRKNGNIGFIIWESHFYEVEMFYSQVFLGMEYAARKSDSYILLTTVK
                     DQFDPKTDLPRFLQCNDVDGVALAGRVPHELVYYLEKERIPFVLIDYSIPGKKYNSVQ
                     IDNYNGAYSAVQHLVELGRKRIGFVGGTFFHSSIKERYRGYKDCIETNKVMTADDISK
                     YTYVEDTETSPAIGEKGISSLLKNDAKLDAVFCCNDPTAMGVIKEIQRRQLKIPEDIA
                     VVGFDDIPNSSYSTPKLTTVHVPKLALGKEAFNLLNKVIEHPLEPPQTRVIATTLVKR
                     "
     assembly_gap    37205..37304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37305..39076
                     /locus_tag="PLI55_00665"
     CDS             <37305..39076
                     /locus_tag="PLI55_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPA99382.1"
                     /translation="ERLQYFLSICEENELYTLPTFIVGHMSGEDWGMPWLKGSDLLKD
                     RRVVDAIKFYITTVIKAINNFKYIQAWLLSNELPNFVGKQEPDDVTAWVEEIIATIRA
                     IDSQRPISIGDGAWAPEIIADQTGFHLRKLNLYQDFVGLHFYPRGMSPWHHSYTTAFR
                     IQLAKVWGKPVLVEEFGTSTTLCSEINQANYYRNVFYSALINGAQGVLNWCLNDFDFK
                     DKRPYSHHLFEERFGIIRTDQSLKPAAYEFQKLEKITGDLITGKYNKVEYNSGLFIPS
                     SYYYNYPYMFKVEGENHYDFFLECFSLLKRANLDVQMVVEPAQELKNDGCYSHALDLK
                     PEDLPVLFLPGLRFMTKQTRIALENYVRDGGVIYFSFANDSWVTDWDKLAGVVTDCKF
                     GVPDFRVKTDLTIKVEDEWGKFAKNEIFTIPLLNNQPALSYCPIISTTARVIMTDEYG
                     MPFLLENAIGKGKVYFTSYPLEALALVINDDHLRAILCRIYKSINRLHGKRAEITIEG
                     DGLELGVWENQAEYRVIVFNHSWQRQNGALYIVHTPLSISSDESDLRTEEEGLYYLTL
                     PRKGVAYLKIQNELKSHKNKQQT"
     gene            39086..41590
                     /locus_tag="PLI55_00670"
     CDS             39086..41590
                     /locus_tag="PLI55_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPA99383.1"
                     /translation="MKKTCLLTMVVGLILLNFAFGDNLIGNGSFEDQSPAFWSPLNGT
                     FGEAVDISSTEHFAGFHSFEITKSAASSDVVGWVSDNNATLYWNNATAGTYALTGYVK
                     TVGVNTNPASDAEKVGIVYEFKNASGGELTTETVWADQSVADKDWTKLTGVAILTEAP
                     ASVVVKLVMGKEATGTVYFDNIDCNTTDTWTMGIFNYGAEDVNGWMDWYSENGSYAVV
                     TDAEAHTGSYSVVMEQPDTCSQLNELVYYSKPYPVEAGEWYKIGVWVKTENVLDSSEF
                     EPTYITKEGIPERISLCYFFHGDADIETAWDKLPGGDKFVYIDQREASTGWTHYVVAE
                     QAPAGDEPATGISVRARFNNKVTGKAWFDDFTVEKIIIDGDNLIGNGSFEDQSPAFWS
                     PLNGTFGEAVDISSTEHFAGFHSFEITKSAASSDVVGWVSDNNATLYWNNATAGTYAL
                     TGYVKTVGVNTNPASDAEKVGIVYEFKNASGGELTTETVWADQSVADKDWTKLTGVAI
                     LTEAPASVVVKLVMGKEATGTVYFDNIDCNTTDTWTMGIFNYGAEDVNGWMDWYSENG
                     SYAVVTDAEAHTGSYSVVMEQPDTCSQLNELVYYSKPYPVEAGEWYKIGVWVKTENVL
                     DSSEFEPTYITKEGIPERISLCYFFHGDADIETAWDKLPGGDKFVYIDQREASTGWTH
                     YVVAEQAPAGDEPATGISVRARFNNKVTGKAWFDDFTVEKMKVAPGTGIITDKGFGGD
                     IPVKYAIMDNYPNPFNPETRIVYSLPQSGKVNLTVYNILGQKVRNLTDTVQNAGTFTL
                     TWDARNDFGQDVPTGIYIAVLTINNQRVTRKMVLMR"
     gene            41665..44316
                     /locus_tag="PLI55_00675"
     CDS             41665..44316
                     /locus_tag="PLI55_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA99384.1"
                     /translation="MKRTTILIIILVILYISLLEAGTTGKIKGRVTDKISGEPLVGVN
                     IVLEGTSQGAATDIEGNYVILNVLPGNYSVKASMIGYQTVIQKNVQVSVDLTTPLDFE
                     MVSTILEGEEVVVIAQRPLIRKDVTSTSFRVSSEQIDQLQVQNLSEIVELQAGVVQGH
                     FRGGRAGEVMYIIDGVPMNDAYAGEHLFDVESDMIQEVEVISGTFNAEYGQAMSGIVN
                     IVTKEGQEYYSGKITFYCGDHLSSHTHIFMNIDDINPLDNTNYQFNLSGPIPLLGKNL
                     SFSLLGRTNTDDGWLYGQRIFRPSDFCYFSDDPNKQIIQSTGDSSFVSMNSSNMQTLQ
                     GKLSFKLSRMDKINYTGFYEKSKYRSYDRLFKYNPDGNYRHENMSYQNSLQYTHMFNP
                     NTFLTANYSNLFTTYGQYVYKDKYDPRYVPIEYLNATNSNGFSTGGMRMWHHFRDNRT
                     EIIKADLRSQITEHQNIGAGVSYKKVHLSLHEYQIYFDENDNIQIPSDSSCYNNSYLH
                     KPYEISAYLQDKIEFGDMIINVGLRYDYFEPDGVVPTQFYNTVGAPKRKAKSSYQFSP
                     RIGIAYPISDQGVIHFSYGHFFQIPNYEYLYANPDFEVSLVQLEGSEPPSNGFNVMGN
                     AELKPEKTVSYEVGLKQAITDNLTIELTEYNKDIRDLVGQLTMTDIYGGKFWRYINRD
                     YANVKGITMAIEMLEKPGGIGFSVDYTYQSATGNASDPLDEYRNQGSDPPIESEKKRQ
                     PLNWDQTHSLNLTTTTTQKGFHICLIGKIGSGTPYTRESPYYNNRILNGERKPMTITF
                     NLNLSKDIPFKNLMISPFIRITNLFDRLNNLEVYPSSGTADYNYSMIFENYRGYKTQE
                     EWNIQPNWYEEPRNVIIGCSISFDQKK"
     gene            44328..47537
                     /locus_tag="PLI55_00680"
     CDS             44328..47537
                     /locus_tag="PLI55_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99385.1"
                     /translation="MNKKLFFKSLLIIMLFLGVTALAQTPSSLRGHRMYRRRGLMNGN
                     LVSTLFWNYCEVGDYPDEPSGCWPSAERHYLDDITLIVSVKTENRNGETIYPMETQFR
                     EFVDISPDGIPWGFEPQPFWFNMDEQYNTSPAMSNNPNTWPDYWPDQDMSWNGYWNGY
                     FGRGVLNADLETIFVFDDDQDKEPNVKWNFYCDERDTTRGGIGLVVKARGFQWSQVLA
                     EDCIFWLYDIKNESTHDYLKCYFAQYIDWGIGGVGGDVQNIGEYDTDLDIAFAYGPPG
                     AVGTPGNWYPIGYAGYAFLESPGISNDGKDNDNDGMIDESRVSDGPGVYISDYPYGFT
                     DAQRAKFIEVYNYEPRAHWSADEDCDWTPFTDINGNGVWDPDEPLNDDKGKDGVGPYD
                     PQYFGPDEGEGDGIPTDGEPNYNATDPDESDQIGLTGFEIFPTHTYELTNDEENYHVF
                     ERSPKPVDQLLQPNNLSMFFSSGPFPLMRNSIERYSMALLFGEDRDDLFKNKKTVQQI
                     YNSDYSFAKPPLKPNLKVYPGDGEVTLVWDDIAESSYDPFLQEYDFEGYLIYRGTTPD
                     FLESKVITDAYGNTTYRKPIAQFDLQDGKKGPHQVAIYGVQFNLGEDTGLRHTYTDRD
                     VKNGQTYYYAVVSYDYGLYHYSESGVEGLPPSECSCIIGTNSLGQVTFLDVNCGMAIP
                     RPASAGYIPPSLDGEIAHVGPGTGSIAIDLVEKHKIPSGLTTYEILFLEDSKYHTETH
                     PWYQVRNVVADTLVLDSTYVKSSGAESPVLDGYTVTVYNDTNITLDEDKSGFIEGTSD
                     YILKVRLNPDNENVAGYRMNLELPADFLVIFHSDFYKNSLKLWSYYSCPSNVQVYNLT
                     DSVEMDYAIIDNDSSLTYTSGDDIALIIPDDSFILKRYTSWMIRLSPLFEIDTSWVNG
                     VPYADTIWTTIDPPEPGEKLFIKMKKPFREGDIFRFSLHGADSSDVLAKAELDNICVV
                     PNPYVVTASWEPANTYKFGRGERRLHFYHLPRQCTIRIYNVRGHLIDTIVHNSTADNG
                     MEPWDILSKEDNEISYGIYIYHVEAPGIGTKIGKFALIK"
     gene            47534..48931
                     /locus_tag="PLI55_00685"
     CDS             47534..48931
                     /locus_tag="PLI55_00685"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPA99386.1"
                     /translation="MIKTSGLIKHFLLVSLLIDIYLFNGCAQKTEENIVAKVADRTIT
                     ADEFAFAYEMAPRSITSLTSKQARQAVLDQLIDRIVLARQAERLRLDDDTLLQRTVDF
                     YKRLAVNRELYLKHIRRLVEVSEQEERQAFEYSKTKLYVRHFSTKQFDLARQVSLGIV
                     QFEHTPIFTGAPQVELTDYGIVDKISWRDIPQEVANILYNLPLRQYSDPFFDGQRYHV
                     FQVVEFEKEVLLRESDFQANRQSLNGIIRQRKEQKLSAGYVQKIMTRQNVIIKAEALN
                     RLTEEVWRNRPTEVVTLEQFITNREINTIGESSRDFLNKPIATFKSGEMTVADILINY
                     KVNPVKISYKSKMTVRESLKNAVGMYVRDWVLSEQGMKERLDRKPSVIAEERSRSEYL
                     LMRKMLAYLGREFYAQNQDTTGRQEQLEKYIANYEDNLRRAENIEIYSSNLMAVKTTD
                     EGLARKIDFVVFHTQ"
     gene            49003..49971
                     /locus_tag="PLI55_00690"
     CDS             49003..49971
                     /locus_tag="PLI55_00690"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPA99387.1"
                     /translation="MGSSKRLTNVGTTAAPFLEVGVGSRAIGMGGAFVGVANDVSALY
                     WNPAGISRITSPEAVFEKVEWLADISFNYIGAVIPFGRWGTAGFFLDAMTVPKMAVRT
                     VDYPEGTGEMFDASSIAFGLSYSYALTDRFSIGLNGKYIEERIWHERSHGVAFDIGTL
                     YKTSFSGLRIGATITNFGTSMSLDGSDLIIYYDADESIDGNNDRIMGKLMTDEWPLPL
                     NMQFGLAYDLLSTKYIKMTIAADAFHPINNSESINTGTELAIYDMLFIRAGYQAIGQT
                     NTEEGLTFGVGLRYKLFGQSYIKFDYAFADFGRLKNVSRYTLRLDF"
     gene            50044..>53028
                     /locus_tag="PLI55_00695"
     CDS             50044..>53028
                     /locus_tag="PLI55_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laminin G"
                     /protein_id="HPA99388.1"
                     /translation="MSRKPLIILFLILNMFTSIKAQQININRVEQMPNLPTPYFMRDW
                     KEVSRGYDRLVFDLNRTGQYLPLVWLNNNTVNYPNHSSFGLHTVVGTTYPSNAEAINV
                     LPAVIGATLVGIDKSDQNGYNWVLGCEEWFNRRPEENVYLNSPTAQSGDDWWYAVMPN
                     VFFYQLYDLYPGTGDFDYQFTSVADQWLRAVVAMGGSAAPWQKPNMNYRGWYLSTMKP
                     FTSGVPEPEAAGAIAWLLYNAYTKTGEAKYRIGAEWCLEFLNAFSANPSYELQLPYGV
                     YVAARMNAEQGTNYNIEKLLNWCFDPENNLRNWGVTLGKWGDYDCYGLVGEAKNEGYA
                     FAMNGFEMAGALVPMVRYDSRFARAIGKWVLNLANASRLFYSPFLPDSLQDGETWAKE
                     YDPEGYIAHESMREIHPNWGYSPFATGDAISGGWGATNFALYGSSHVGILGGIIDTTN
                     VTGILKLDLRRTDYYQKDAYPSYLFYNPYSEVKSISFNVGTQAVDLYDAVSKTFIVTG
                     ITGETNLNIPADGAVLVVLVPSGGTVRYVEEKMLVNDIVVDYHSGQTVTNYKPRIKAI
                     ASDSTALKAGRIYKIYCTAVDRDDDELHYSWQINDSEIGCDSSIIELTMPELNTTLTI
                     ICRVSDEVNTPVADSIQILVYTVQNQVPIISSLTASARRIAPTDTTLINCSASDPDGD
                     SLIFNWSAASGILESDSAQAVWQAPDSSGYFLIKCTVSDLFGGVALDSILIVVGDTSG
                     HEIGAPLAYFAFAGNADDLSGFNNHGTVNGASLSADRFGKLNSAYYFNGTSDNIRVPN
                     SVSLNFTKAITVSFWINAAELYTSRESYPISHGNWENRWKVSIIPQKKIRWTIKTTTG
                     VKDLDSDKTINVNQWYHIVGLYNGIDFDLYVDGELSNHTTFSGDILTTNIDLMIGQCL
                     PAVSQYNFKGVIDDIRIYDYALSIGEISKLYTEPSAIRSDTKEMPTSYKLSQNYPNPF
                     NPVTTIKYDLPQNVPVKLQVFDL"
     assembly_gap    53029..53128
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <53129..53339
                     /locus_tag="PLI55_00700"
     CDS             <53129..53339
                     /locus_tag="PLI55_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPA99389.1"
                     /translation="IKYDLPQNVPVKLQVFDLGGRLVQTLVDEEKPAGHYTIVWDTLN
                     ISSGIYFYRLQAGEFTSVQKCIKLK"
     gene            53356..55845
                     /locus_tag="PLI55_00705"
     CDS             53356..55845
                     /locus_tag="PLI55_00705"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99390.1"
                     /translation="MSKTHLKSIFYLTLILTLTSFFSCSPKSSIGTDKLAVQFKGDAK
                     IEVGGPFVGAEFHHSYMIPQRISFFYPVANSIDLSRDYWTRDSSFVADWTLKIGNSAD
                     IEIGKKPAEFDLTPYSVDFHYKDDEYIVKAAYRFCNEKPAMVVEIEIVNNSGIAQDFQ
                     FDTRLRTSIRTCHTFREIDKAVSEIEDNTIYNNFYDADADSAVVFITNDGDLPVECLT
                     ANVVQLPFAGFIYKKGLKPGESLNIIQIIGSSNPEEGKNLVKYLRANYQLEIDQYEKY
                     VNEQAFLSSLMKTGSPATDHSVAYAKAVMSANAHYLDGEIVPMPCPAEYNFYFTHDAL
                     VTDLAAVNFDLARVRRDLDYIVRHSDENNVIPHAYYWKDGKYVTEFASSDNWNNFWFI
                     QVAAKYLRHSGDSDFLTQLYPYLTVSVERSLLTLEKDDLMWSYRPDWWDIGHNYGPRS
                     YMTILAIKSLRDYAYISTVLGKNPEKVVELMALADKMQTALVAKLWNDDMGYLINYNE
                     DGSLDSHYYIGSLLAVHYGLLDKEKQNRLVQTAMAKLLDEKVGIYNVYPMDFHLLGDF
                     YKFVGNEVGDKYYYANGGVWPQGNAWYALALIANDEKEKAAGFIENTMSLYGIMNGPN
                     GQPAYYEVRNANKEDPTEYGTVDKPCFLWAGAWYISSLYRLYGITENDWNIALDPYLP
                     NDQNNCQFTLFFNGQPLVIEIKGRGKIIHSLKFDSQLAHSAVFPIDMPVAKKIEVILS
                     DSPKEPYLKSTNAILLKYEYQNRLLSLMLRGIAPLENETIVISPQKPVSISLNNRMLT
                     EGWSTDKADGWYVTKIRFRMTASDDKLCVKF"
     gene            55855..57171
                     /locus_tag="PLI55_00710"
     CDS             55855..57171
                     /locus_tag="PLI55_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPA99391.1"
                     /translation="MIYNKTTVVIFLLFLVIEVSCERKQAAQFKDEQDIIRLTYWCAS
                     NQDEINLAKELVNEWNAEHPKVQVVLQPIPASQSSEEALLAAIAGKTTPDVCSNMWPG
                     AMDDFTSSGGLVRLDQFPDFIEYMQKRMPEELLESFRSPDSHYYQIPWKTNPIMVMYN
                     KKMFREAGIVRPPRTYSEYLAAAEKITKDINGDGRIDRWMGYRDIRPIWWQRLFDYYP
                     LYIAASGGMTLFSGSEIAFANEASVKVFQFFNELYRYGYYPLTTFQGDAFLAEQVATQ
                     FTGPWNVIHVENFKPPGFEYGISPIPVPDDYNGPVYTYGDHKNISIFSTTEHPEAAWQ
                     FTKFLISAQADLRLLEICSQIPLRKNLTVVPLYHEYFLENPMMMQFAEQAVYTRGVDG
                     SSVLKEIFDAISQEYEICAVYNKRMPQEAVANAAKRAQIIIEWNRK"
     gene            57171..>57504
                     /locus_tag="PLI55_00715"
     CDS             57171..>57504
                     /locus_tag="PLI55_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPA99392.1"
                     /translation="MKAGFPVNKIKNKTGYLMSLPYILYFCLFIGFPLIFSFILIFHK
                     WNIVTPMQWVGLRNFYRLFHDVQFFTSIRNTLIFLVIHIPLQIVIALFLAVILNQKIK
                     FKGFFRALY"
BASE COUNT        16451 a        10590 c        13043 g        16620 t
ORIGIN      
        1 agctattcgg cgaatgtggg caacgcgatt tatattgata attttcggat agtatatctt
       61 gatgagatca aaaagccagt agttgtcgaa gccgaatctg gagttgtggg aagtgatttc
      121 agtatattag aggaagattc gattacttat gtgactattc aaacgaattt agcagcttac
      181 aatcctggca gtagtgcccg ggtaatatcc tacgaagtaa ctttcccaga taccgggagc
      241 tatgaccttt ttgtccgttt acgaacggga gcagatacat ttaacgatga cagcttcttc
      301 tatggcaaag gatttggaga aaaagatccc tcagttgatt ctgtttgggt ttgtataaat
      361 ggacttgcgg ctgccggatt ctcaggaccg aatgaaattg ttcgtgacgc aggcggtctt
      421 ggttctgggg tttggaaatg ggttaatctt tcacgaaatg tatatcaggg ttccgttgag
      481 agccttgtta cttttattgt tgacgatcca gatagtttaa ctcaaacttt tcaaattggc
      541 gcacgcgaaa atggattgga tattgacaag attgcctttg gtaagtcata tctatattat
      601 acggtaggaa atcttgataa tagagaaccc ggttcaacag acctgccagg tgaagtatgg
      661 gaaggtccac cgctggctca taaacaacca aaatttgttg gaaatattta cagttctccg
      721 cagataagta atttcgaagc ttactggaac caggttactc cagaaaatgc cggcaaatgg
      781 ggtagtgtgg agggcactcg tgacgttatg aattggagcg gattagatgc tgcctataaa
      841 cttgccaagg ataatggttt cccgtttcat tttcacgtgc tcgtttgggg tgctcagcaa
      901 cctagctgga taaacgattt aacacctgaa gagcaacttg aagaaattag agaatggttc
      961 gaagccgttg ccgaaagata tcccgatatt gattttctgg aggtggtcaa tgaaccattg
     1021 cctggtcata atccgccgga tggaactagt ggacgagcta actataagga ggctttgggc
     1081 ggtaacggtg aaaccggttg ggattgggtg attaaggcat ttgaaatggc acgtgagatt
     1141 ttccccgaag ggactagact gatgataaat gattttaata taatcagtag ctcaagcagt
     1201 acatcccaat atcttaagct cattagattg ctccaagaga gaaatttaat cgatataatt
     1261 ggcgaacaag ggcatgcatt tactacaact gccgctgtag ccacaatgaa aaaaaacctc
     1321 gattctcttg cttctaccgg attaccaatt caaattactg aattagatat tgatggacct
     1381 tctgatgcgg tccaattaca aagttatcaa agaattttcc cgactcttta tgagcaccct
     1441 ggagtagaag gaattacctt gtggggctgg cgacggggtc tttggagaga cgaacaggga
     1501 gcttacctga ttaatcagga tggttcggaa agaccggctt tggtatggtt aagaacctat
     1561 ctggatactt taaacctcaa cgttatggtc aaagaagaca ttattaacgt tctcgatgaa
     1621 tttagcttag gtcaaaatta tcccaatccc ttcaatccga cgaccaccat caattatcaa
     1681 ttacctacta ctagtcactt gactatcaaa gtgtataacc tgctcggaaa agaagttgcc
     1741 actttggttg atgatgtcaa acaagccggt agtcactcgg ttacgtttga tgggagtgga
     1801 ttagcaggcg gagtctatct atatcagtta aaaacggcta actataccga cacaaaaaaa
     1861 tgcctgttct tgaagtagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     1981 agtttagcac ccgggatgat tcgcggataa ccagatcggt tcccagaaca attttctcat
     2041 tggggatagc ggttgaggac tcgatattgc gaatcaggat ttcggcggct ttataaccga
     2101 tttcgcgctg aggcgcccga atcgtggtca gaggtaccgg atagatttta gcgtagataa
     2161 tatcatcatt gccgattatg gaaatatctt ccggaattcg gatattcaat tccttaagag
     2221 cagtcataac cgccagagct tgctgatcat tgaaacaaac tatagcggtt ggataatctt
     2281 cccggcttcg gttcttaaaa taattcatag tattaccata ggcttcttca tgacgcgaac
     2341 ctatggatat gatcaaattt ttattgaaaa taagggggct ttcactaaaa gcatgacgaa
     2401 agccctcaat ccgttcctga gtatgcgagg actggggcgg tcccgcaaaa tggacaatct
     2461 tggtgtgccc attatcaatt aaatatttga ctgccgtttt aatggcggat aggttgtcaa
     2521 tggcaaccac attagcctga atacctttga catcttcaag tagaacaaag ggatagttga
     2581 ttctttttaa tttgaaaaga tggtcaattt ccgcctcacc ttcgacgatt ggcgctatta
     2641 tcgcgccttt aatgtctttg atagaaaaca agtgcgtaaa ttttttctcg cattcatggt
     2701 cattttccga actggcgaca atcaccgaat acccttgact attagcatat tctctggcgc
     2761 ccgccgcaat cgaagtgtaa aatgggtaat tcaagtcttt aatgattata ccgatacagc
     2821 gaacttgatc accgtttttc aggttccgag ccatacctcg gggacgaaag ttaaggtctt
     2881 tcattacctt aagaatatgt tctcgcgtag cgggattgac agaattctta ccatttatta
     2941 cagcagatac cgttccttta gaaacgttgg cctgcttagc aacttcttca attgtaattc
     3001 tcgtcataga aaactccggt tcataatcat acgatccata agtgagtcca aattttttgg
     3061 tttaagaaaa aactcattgc cgccaatact gacacttcgt ttatagttgt aaagaaaaaa
     3121 tagccgtaat accatacctg accgattcca atcaccaaaa aaatcaactg ctaatttaag
     3181 caaaattttc atagaatggt attaattata gtaacccatt ttatcatatt ttcattcatt
     3241 tgagattttg ataaaattct annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 ntatagtaac ccattttatc atattttcat tcatttgaga ttttgataaa attctatcaa
     3421 gtatctttaa gttctattaa gaaattttta gtcctaaaat tggtttttaa acatcaatca
     3481 atgcgcggtt gcatgaaaat acttaagagg aattaaaaaa actctgtgtt ttccatatcc
     3541 tcttcgtgcc cccgtgtcgc aatttaagga cattgaatca ggcaagttga attttccaat
     3601 gaaatcttgc tgtttctgcc ggatatagtt cattatacaa aattaatttt taaaaatgca
     3661 ctatctattt gattaaacgc ctagatatta ttaaaattaa aacggttcaa taaatgtaat
     3721 attggttgtt ttgataaagg aaaatataaa tggaggacaa aatgaatcaa atttcgcgaa
     3781 atgtgattcg ttttaattgt tgcaaatcgt tcacttcatt gataactcat ttcgcaaagc
     3841 aagtaacgtt aattctcctt tccttattaa tattatgccc taatatttta gaagctgtta
     3901 ccagtgatat ggattcctat atttcggtag aagcgattgg cggcggtttt ccgctttcag
     3961 tggctgggca taccgcgcca ctatatgcca gctcgcagga ttatccggga gtattgcgag
     4021 ttctgaagct attccaatca gacctaaaaa tggtaactgg catcgagccg gtgattgcca
     4081 ttgatacgat tcccaagtct aaggaaatcg tcatcgtggg caccataggt aaaagtccca
     4141 taattgatgc tttgattcaa agtaatcaat tggatgtcag tgatatcgcc ggcaagtggg
     4201 aaacttttct aattcagaga atagaaaagc cgtatccgga tgttgagcaa gcgttggtta
     4261 tagccggtag cgataagcgc ggcacaattt ttggtatgtt cgatctttcc gagaaaatcg
     4321 gggtctcgcc ctggtattgg tgggctgatg ttccagtaaa gaaaaaggac tgtatttata
     4381 ttaaaccagg acgacatact ctcggtgagc ccaaagtaaa atatcgggga ttttttatca
     4441 atgacgaagc gccggctcta agcaattggg cttacgaaaa attcggcggt ttcaatcacg
     4501 aattctatgt taatgttttt gaacttctgc taaggctaaa agctaatcat ctctggccag
     4561 gtatgtgggg caaatacttt ggctccgacc cggagaatcc aagattagcc gatgaatatg
     4621 gtatcgtaat gggctcgtcg cactgtgaac ctctgcttta taataatgat cccggtgccg
     4681 gtttatggaa ttcacaaact atgggaccct ggcgttatga tattaatcgt gaaaatattt
     4741 ataaagtgtt ggatgaaacc gttgccgctc gtggccaata tgaaaatatt tatacggtag
     4801 gtttacgggg aattcacgat acccagatgg aagggggagt ggatgttcag gaacaaatcg
     4861 ccttgcttga acaagtattc aaagaccagc gcgaaattct tacaaaacat ataaataaag
     4921 aaattacgga aattccgcag gtttttattc cttataaaga agtgcaggat tattacgatg
     4981 ctggtttgca ggttcccgac gatgtgacta taatgtggag tgatgataac tggggcaata
     5041 tccgtcgcct gcctcgattt gacgacaaac cacgcgctgg cggatatgga atttattatc
     5101 attttgatta tgttggcgat ccgcgcaatt acaaatggtt gaatacggtt caaatttcac
     5161 gagtttggga acaaatgcac ctggcttatc gatatggtgt tgaccgaatc tgggtggtca
     5221 atgttggtga cattaaaccg atggaatttc cgattaactt tttcatggat tttgcctgga
     5281 atccggaagc ctggccggcg gagcgcttgc ccgaatatac tcgcctctgg gcggaggagc
     5341 agttcggcgc tcaatatgct gaggaaattg ctcatatatt gactcagtac acccgattca
     5401 atgcgcgccg caaaccagaa ttactctcac ctgaaactta tagtttggtt aactatcggg
     5461 aagccgaaac cgttgtggct gaatataatg acctggcaaa aaaagcacgg gaaatttacg
     5521 aagctctgcc gactgaattt cacgatgctt tctatcaatt agttttgcat cctaccgaag
     5581 cctgcgccaa tctcaatgag ctatatgtga ccgtggcgcg gaaccgactt tatgctcacc
     5641 agaaacgcgc cctgaccaat gaattagctc aaagagcccg tgatctattt aagaaggatg
     5701 ccgaaataac cgatcgttat cataatatcg ctaacgggaa gtggaatcat atgatggatc
     5761 agactcatat aggctatact tattggcaac aacccgaaga aaacacaatg ccagaagtaa
     5821 tcgaaatcaa tgtaccggaa tcagccgaga tgggtgttgc gattgaaggc tcaaccgaat
     5881 gttggccgcc tgcgaatagc gaggcgattt tgccggaatt cgatccctac aatcaatcct
     5941 gtcattatat cgaaattttt aatcggggta aaaagccttt taaatacacg attaaatccg
     6001 gcaatccctg cgttctaatt agccagaaaa aaggagaaat tactaccgaa gaacggctct
     6061 ggattgaaat tgattggaag caagcgccca aagggaagag cacaattccg ctcattatca
     6121 aaggtcctcg gaaaagccag gtggaggtga aaataattat ctttaatccg gcagttcctg
     6181 tagaggagct tgccgaccgt tttcttgaga gcaacagata tgtttcaata gaagctgagc
     6241 atttctccaa tgccgtcaac acgcgtggtg tttactggca aataattccc gatttgggac
     6301 gtacgctttc ctcagttact ccatttccgg tgacgatgtc cagtctgata ccggaaggcg
     6361 atagcccacg tcttgaatat caggtttatt tgtttaatgc tggtgaagtg aaagttaagg
     6421 cttatctggc tccaacccag aattttccca atggtccggg cttacgctat gggatttctt
     6481 ttgatgatga gccggttcaa atcatcaata tgcatgctgg caaaacccac cgtgactggc
     6541 aaaattccgt cagtgactat attacgataa tgacttccgt ccacgcgttg aagtcgcccg
     6601 gcaaacatac cttaaaattc tgggcggtcg atcccggcgt ggtactccaa aaattggtaa
     6661 ttgagactgg tgaagtaaaa cccagttacc tcgggccgcc ggaaagccgc tggtttaaaa
     6721 ttgcagaata aaagttttca attctaaaag tgcaggaatc aaatgaaaaa agtgataatt
     6781 acaatgacgc taatagttgc gagttttata agcgccgacg ctttttcagc tgatcaaaaa
     6841 gcgttctact ataatccaat tctggctggc ttttatcccg atccgagcat ttgtcgggtt
     6901 ggagatgact attatctggt taattccacc tttgcttatt tcccgggaat accggttttc
     6961 catagtcgcg acttagtcaa ttggaaactg attggtcatg taatggatcg agtggagcag
     7021 atgaatctgg aagggttggg cgtgtccgaa ggaattttcg ctccggcgat tcgatttcac
     7081 gatggattat tttatgtaac ctgtactctg gttggtgccg gtggaaatta cgtcgttact
     7141 gctcaaaatc cggcgggacc gtggtcgaat ccggtttggc ttccacaaat tgacggcatt
     7201 gatccctcgc tatttttcga tcaagatggc aaagcgtata tcgtttataa cagcgtcgcg
     7261 ccgaacaata gacctttata cgaagggcat cgcacgatcc ggatgtatca atttgactac
     7321 gaaaaactta aggtaattag tcctgagatt atcctggtga atggtggtac cgatatttct
     7381 aaaaagccga tctggatcga gggaccgcat atttttcgca aggataattt ctattatctg
     7441 atttgtgccg aaggaggtac atcttgggaa cattccgagg tagtctttcg gagcgaacag
     7501 gttaccggac cgtatgttcc ttatgcgaaa aatccgattc tgactcaacg tcatcttgat
     7561 ccgaatcgtc ctcatccgat tacctgtacc ggtcacgccg atttggtgat gacgcccgct
     7621 ggcgattggt gggctgtatt tctcggctgt cgaccttatc ttcccgaata tgaaggtttt
     7681 ttcaatactg gtcgagaaac gtttatggca ccagtaaaat ggattgacgg ctggccagtt
     7741 attgatcctg accacaacga gattcaatat tcctatcctc tgccgattat attggaaaaa
     7801 ccaatcaaag ctgatattcc ctatagtggt aatttccgaa tcagagatga ttttgattcc
     7861 gaaaagcttg acttgaactg ggtctttctc cggacgccgc ataccaagtg gtatagtcta
     7921 agtgatcgta gaggttcgct ggctattcaa ttaagaccag aaacctgctc cggcaaaagc
     7981 aatccgagtt ttatcgggcg gcgtcagcaa catttacgcg ggtcggcgag tacggcaatg
     8041 gattttgttc cggtttcgga atttgaaaaa gccggattgc tggtttttca gaacgaaaag
     8101 cactattatt ttttaggcaa atcaatagag aaaggcgaac cggttgtgca attatataaa
     8161 accgacacat tgctggcttc acacaaaatc ggcgaggggc gtaatatccg gttgaaggtt
     8221 gtcgccgatg gcgccgaata ttctttttac tacgccacca agccagaaga atggattctg
     8281 ctgaaagaca aggtggatgc gcgctttcta agcacacgag tggcgggcgg atttgtgggc
     8341 tgtatgtatg ctatgtatgc aacttcgtca ggcgaagcca gttctaatgt tgcctatttc
     8401 gattggtttg aatatgtggg tgacgatgaa atttaccatc aataatactt aatgaggatt
     8461 aaaaatgaca gatcaacagt gggaaactct caaaaaagta atcaatggcg aaaaaataga
     8521 acctttgccg gttggattta ttgctgacag tccctggttg cctggctggt atggtatcaa
     8581 aattctcgat tatttttcca atgacgaatt gtggttgaaa gccaatctca aagctattca
     8641 agaatttccg gaagttatgt ttctgccggg gttttggtcg gaattcggaa tgtgcaccga
     8701 gccgtcggct tttggagcac gttgtcggtt cccgcaaaat gaatttccac atgcccatcc
     8761 ggtcatcaaa tcacccgagg agattaatgc gctggttgtg cctgaaccgg aaaccgatgg
     8821 tttattgcca ttcgtcctga atcgtctcaa attagcccaa ccatacatcg aaaatgccgg
     8881 tcacaaaatc cggtttgcca tagcacgagg gccattgaac attgccagtt ttttgatggg
     8941 cacgactgaa tttttaatgg caatgctgac gagcccagag ttggtcgaaa cattaatgga
     9001 aaaaataacc gcctttctga aaaattggct ccggcttcag cgaagtacat tcagtacaat
     9061 agacggcatt cttctgctgg atgacattat cggttttatc ggggaacagg aattttgccg
     9121 atttggatta ccctatttta aggagttatt tagtgccgat gtgtcggtga aatttttgca
     9181 caatgacgct caatgccggg tttccgtgcc atatttacca gagataggtg tcaatttatt
     9241 taatatggga tttgacgtca gcctgaatga tatcaaaacc tggactaata atcaggtgac
     9301 gctacttggc aatatcccgc cgcgcgatgt gctagcaaac gggaaaccgg aagatgtcgt
     9361 aaaaagtgta accgagttat tagattcact tgaagatcaa acccgggtga tattatcttg
     9421 cggaggcggc ttgccaccgg gagtgagtac ggaaaacctc aaagctttta tcgagacggt
     9481 gaaaaattat acgcttaagt aaattatcac gcttgatttt cccgaattgt aggaatgaaa
     9541 aatcgatcta attttaaaat atgagtccta atgaataaac gctctaatat tttgttttta
     9601 aattggcatc cgccttttct tttaggaatt gtaattgcat attttgcaat atcagtccgc
     9661 tttttaccag ccgctgaaaa ggttgattca gaaaaagtca ttcgtaaaat tgctgataat
     9721 gtcctgaagg atgccaactt taattttatt gatcaagaaa gcggcaaacg ttattcttca
     9781 actgccgatg ccccgtccgg agcccggttg gttccagaaa gtcgctacaa cgattggcgc
     9841 tactggaacg gcgttttgaa tatcgctatg ctcaggctgg gcgaagtgct gaaggagccg
     9901 acttataccg aatttgctgt ccgaaatgtc gcatttagtt ttgacaatta tgcctatttt
     9961 gaaaaacgat atgccaatga aaataaatgg gaataccctt tcggtcagtt ttttatgatg
    10021 gaggaactcg atgattgtgg agcgatgggg gctagcgtta tcgagataat gcgtctcgat
    10081 ccgcaggaac ggtatctcaa atatattgat cgagcggcaa cgcatatttt aacccgtcag
    10141 agtcggttgg aagacggcac gctcgtgaga acttttccgc gaaaatatac tctctgggca
    10201 gatgatttat atatggctaa ttcctttctg tgtcgaatgg gtaaagtgac tgaacgttcc
    10261 tggcaaactg ttggaattga tgatgccacc cgtcaggtga tcaatttcca taaatacttg
    10321 ttcgatcagg aaatgggctt aatgtatcat aactggtatt cggataccgg gagtccggga
    10381 gttgctttct ggggacgcgc caatggttgg gcgattttgg ctcaggttga gctactggat
    10441 cagctcccag ccgactatcc tcagagagat accttgctgg cgttatttcg gtgccatatt
    10501 gacggaattg tcccttatca ggactcaacc ggactgtggc atcaattgct cgataaaccg
    10561 gattcatttc cggaaacatc ttgtacggca atgtttactt acgctattgc gcgggctgtt
    10621 aatcaaggct accttgaatc ccgctacaag tcgtttgctt taaaaggctg gcaaggcatt
    10681 ttgaccaaga ttcgtcctga cggacaggtc gaagggattt gtaccggtac atcaacaagc
    10741 gacgatttaa gcgattatta cacacgtcca acgccactca acgatgggca cggtattgga
    10801 actgttttaa tggctggaac tgaaataata caattgctta agtaaaacca aaccatagat
    10861 ttagtgcttg atatgtccca gataaaaatc catcccgaat aacaccttta aaatatttat
    10921 tggtaatatt gaaacggttc aagtaaattt tagattatga tgataatatg taaagatatc
    10981 tataaaaaaa ttttaaccta cccaacttta aatattaata taggcaaata aaactatcaa
    11041 aattgaatat tatgattttt tctattacaa atcatttaac acttaataaa actatgattc
    11101 gtaagaacag taaataaaaa acaggagatt aattatgtca aaacaggtct taattggaaa
    11161 acgggaatat ttcaagggaa tcggtaaaat tccttatgaa ggtcctgaat caaaaaatcc
    11221 tttggcgttc aagtattatg atgagaacag agttgtggct ggcaagaaaa tgaaagacca
    11281 ttttcgcttt gctattgctt actggcatac attctgtaat aatggtggag acccatttgg
    11341 agcggagggt acggttgatt ttccctggaa tgagtctaaa gatccaatgc aagccgccaa
    11401 agacaaactg gacgcggctt ttgaattttt cactaagtta ggcacaccgt tttattgctt
    11461 ccacgatcgt gatatggctc cagaagggaa gaatgtagtc gaatctgaaa agaacctcaa
    11521 agaactggta aaaattgcca aagccaaaca agaagagtcc ggtgtgaaat tgctgtgggg
    11581 cacggctaat gtatttgggc ataaacgcta tactaacggt gccgccacta atcctaattt
    11641 ttacgttatt cctcacgtcg ccgcacaggt gaaagccgct attgatgcaa ccatcgcact
    11701 cggcggagaa aactatgtat tctggggcgg tcgcgaaggt tactacagcc tgcttaatac
    11761 caatatgaaa cgtgaaatcg agcatatggc gatgtttctg accaaggctc gcgattatgg
    11821 ccgggcgtct ggtttcaaag gcacattttt gatcgaacct aaacctatgg aaccaaccaa
    11881 gcatcagtac gatttcgatt cggctacggt aatcggtttt ctgaacaaat acggtcttca
    11941 gaatgacttc aagatcaata tcgagaataa tcacgctacc ctggctgggc atacttttgc
    12001 gcacgaagtc cagacggctt gcgatgccgg cctgttcgga agtctggata ttaaccaagg
    12061 tgatcctttt aatggctggg atactgatga atttttacat aatctgtatg atgctaccga
    12121 actaatgcta attcttttgc agaatggtgg aatcggccac ggcggtatga attttgatgc
    12181 caagaatcgt cgtagttcga ccgatttgga agatattttc attggccata tttactcgat
    12241 ggatactctg gcgcgcggat tgctgattgc caatgatatt ctggaaaaat cggattatct
    12301 caagatgaaa aaagaacgct acagtagttt tgattctggc gacggtgctc gtttcgagaa
    12361 aggtgaatta ggcctcgtcg aactggcgaa aatcgccgaa gaaattggcg aacccaaaat
    12421 gatcagcggc aaacaagaat tgtacgaaag cctgatcaat cagtatattc gctgaatctc
    12481 ataaatagga gtaaaaccgt gtttaaatta ttcgccgggt tggatgtttc gacccaaagt
    12541 accaaacttg tcatcattga cctcgcggct aagaaagtcg tttatgttaa ttcggtcaat
    12601 tacgataccg acctgccgca ctataacacg aagaacggcg ctatccaggg gctgggagaa
    12661 ggtgtctccg aatctgaccc ccgaatgtgg atagaggcgg ttgatcgcgt ttttgctaat
    12721 gccgtcgcgg ctggaattga tatgtctcag atcggttgca ttgcggtttc cggccaacag
    12781 catggtctgg ttgctctgga tgcgcagggc aatttatcgc gacccacaag taaactctgg
    12841 aacgattttt cgactctgga agaatgtgaa attctgacgg aaaaagtcgg tgggctggaa
    12901 caaatgattg ccgaagtcgg caatagccag cgtactggat atactgcggc gaaaattttt
    12961 cacatggtgc gccatgaacc ggaaatttac cataaaacgg caactctctt tctggtgcat
    13021 aattatatta attattatct gaccggaggt gtccgcgtga tggaaccggg tgacacctcc
    13081 ggcatggcac tttggaaccc caaaaatggt cagtggtcgc agaaagtcat ccaagctatc
    13141 gctcctgatt taatgtccaa attaccggaa gttaaacctt ccgatcaacc gattggatat
    13201 atttcttccg aattagtggc gaagtttggt ttttcgaaag actgcctaat tgatgccggt
    13261 tgcggagaca atatgtatgg cgcagtcgga accgggaatg tcgaacctgg gattgtgacc
    13321 gtcagccttg ggacaagtgg aacggcttat acatttatgg aggagcctta cattgatcca
    13381 acgggagaaa ttgccgcttt ttgtgatagc accggtcatt atttaccttt attatgtgta
    13441 tcgaatttag ccaacgggta caatgaattc attaaactgc ataaaatgac tcatcaggtt
    13501 tttgataaaa tgcttgatga aacgccgcca gccaataatg gtaaattact gattccctgg
    13561 tatgtaggcg aacggactcc ggacttgccg ttggctgcgc caatttactt cggttttgga
    13621 ttggctgatt ttacccaggc taatttagct cgtgctatta tcgagggcca tattcttaac
    13681 ctctataccg gttttaagcg tatgccagtg gatgtgaaag agattcgcct gaccggtggg
    13741 ctttcgcggt ctgatacctg gtgccagact attgctgata ttttcgaagc ccagacagta
    13801 ccagtcgaag gtgaaggtgc ggcgctgggt gcggctttgc atgcggcttg gacctggagt
    13861 aaagcccaga ataaggacat aagtcttaaa gaattggtca agccatttgt agttctggac
    13921 gaaaaaaggc gcaaacagcc catcgaagcc aatctaccag tttatcggaa attaagcgcg
    13981 atgtttgaat ctatcagttg ccggattcgc gggttgacag gtgatgatcc tttccgtctc
    14041 agagcggaac tgatcaaact cagtaaaaca aaggcgaata attgaacccc ggcagttatc
    14101 gctaatcgaa aaatcagaaa cacggagcga aaatgaaaaa gtacaagatt tcaaatgata
    14161 aaggagtttc ggcagannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacgg
    14281 agcgaaaatg aaaaagtaca agatttcaaa tgataaagga gtttcggcag aaattatcag
    14341 ttatggagct atcgtagttt ctttgacagc tccgaacaga aatggccaat tggaagacgt
    14401 tattctggga tttgatgatt tagccagtta cgaaaaagat aatactttcc aggggtctat
    14461 tgttggtcgc tatggtaatc gaatagctgg cggaaaattt agtcttgatg gaaaagtgta
    14521 tcagctcaat ttaaatgatg ggccaaatca tttgcacggc ggcccgaaag gttttttcaa
    14581 agtaatatgg gatatcgaac agttggatag ccaatcggtt cggttgactt atgtcagtcc
    14641 ggacggtgaa gaaggctatc ccggggaagt aaaaatatct gttgtttata caattaccga
    14701 tgacgatgct ctgcgaattg actattatgg aacgaccgac cggccgacta ttctaaatcc
    14761 taccagtcac tgctatttca atttaagcgg atcgccgaaa aatactattt tagatcacga
    14821 attaatgttg aatgctgatt tctttacgcc gattgatgaa ttttctattc ctaccggtga
    14881 aatccgaccg gtagctggca ctccgctgga ttttcgcgaa cccaaaccga ttggtcgtga
    14941 catcaacgct gaagatgagc agataaaatt cggcaaaggt tatgatcata actgggtaat
    15001 taacggtaag cctgggcagg tgcgtctcgc cgcaactgtt tatgagtcca agagtggtag
    15061 attgatggaa tgcctgaccg atcaagccgg tttgcaattc tattccggta attttctaga
    15121 cggttcactg atcggaaaag gcggtattcg ttacaattac cgctgtgctc tctgtctgga
    15181 agcacaggct tttccggatt cgcctaataa accgcatttt ccatcggtag ttctgcgacc
    15241 gggcgaagaa tatcgccaga ctaccatcta tcgtttttcg gtaagataga aaatttataa
    15301 gtaccaataa actaatatat tagctcaaga tacgagcaag taaatgttgt cttttatgat
    15361 tgctaaaagg caacatattt tataattggg tcggaataga ttatccaatt attcctgaaa
    15421 caatgtaatt gattcttatg gatttcggaa tatttcgatg aattatcgaa aatattttcg
    15481 taaccactgg agtgtaaatt ttttagttaa tttgaaaatg aacaaaatta gagaaattct
    15541 cgacgataaa atggaattaa aataattttt cttgcatttt taaaaatgtc atttaaatta
    15601 ggatacagaa aacgatttcg taaaagagaa ttgatggatt gaaaagggat aaaggctatc
    15661 gcggggtagg ggaaaatgac cgctagtagc atacaaaaat caggattggt gcggtactat
    15721 gaccggtttt taattacttt aattattgtg acttaggcag atttaacgat gacaattaag
    15781 gatattgcgc gtctcgcgaa ttgttcactc tcgactgtat cgaaagcctt gaatggccgc
    15841 caggatgtga gtgaggctac tcgtcagcga attatgcaaa tagttgctga atataacttc
    15901 actcccagtg cttacggtaa aggcttgaag aaacagaaaa ccgagaatat tggcgtaata
    15961 ttctgtcggg aaacgcgacc gctatcagcg aatccatttt attcacgtgt actggaaggc
    16021 atcgaagctg aattggtcat taataactat aatttagtgc tgcatttact gccagaagta
    16081 tatcgcggtg aatacccgaa aatgctcaaa gaacataccg tggatgggat aatactggca
    16141 ggtgtactcg atcaggattt tgtggatctg gttaaagaat ccggaattcc aattgttttc
    16201 gttaatccca atttcgaaat cgaaggagaa gatcaaattt tcatagatga tgagcatggt
    16261 gcttttcaag cgattcaata tgtcataagt aaagggcatc agagaattgg ttttgtttcc
    16321 ggtgatttga ataggattag ttttaaaaaa cgttacgatg gatataaaaa ggcattggca
    16381 taccatagaa ttgaatatga tgatgaaatt gtccggacag gaggcttgga aaacggatat
    16441 gatcaggtta cagctttaat gaaattaaag aacaaaccga cggtcatttt ctgcacaaat
    16501 gaccttaatg caatttttgg ttataaagcg attgaagact tagggtatcg tattccccag
    16561 gatatcagta taattggatt tgatgatatt gatctggcaa aaatatccaa tccaccattg
    16621 acaactgtga gagtttataa agaagagatg ggatcaatcg cagttagaat attattggag
    16681 aaaattaata aaactgagca taagcctgtt actactcttt taccggtgag aataattgag
    16741 cgtgattcgg tgaaagacct taacaatctt aaaaataccg ttagacatat agaacccaag
    16801 caagatgtgg agttagtatg aagagatatt taaagcccgc atttagtgca ttagcggtga
    16861 tgttgtttat taatttattg aacgccggag taataagagg gagagtggta gattcaaaaa
    16921 ccggcgatcc gctcattggt gcgaatctga ttttagctga aacagctggt attggagccg
    16981 cttccgattt taagggatat tatagaatag ataatgtacc tgctggcgaa tataccttga
    17041 aggtgacttt tattggttat caaccggtgg aaataaaagt cagtctgaaa gcagacgaaa
    17101 ccatcgttcg cgatatcgag atggtctatg gtggtgcgct aaaaggagaa gaagttgtcg
    17161 tcaccgcgca agccaaagga cagatgtcgg ctatcaacca gcagttagcc tcgaatgtaa
    17221 ttaccaatat tgttgatgag agccgtatcc aggaattgcc ggatgtgaat gcggctgagt
    17281 caatcgggcg tttacccggt gtttctattc agcgttccgg tggcgaagct aataaagttg
    17341 aagttcgagg tcttaatccc aaatatagtt tgattacggt caacggtgtc gaacttccag
    17401 ccaccggttc cgaagaccga agtgtcaacc tttcactaat ttcgtccaat atgctggatg
    17461 gaatcgtttt gaaaaaaacc aatactccag atatggatgc tgatgtttta ggcggcacga
    17521 ttgatttaag actaaaagaa gcgccggaag gttttaagtt taacctgtca gctcaaggtg
    17581 gatacaatca acttcagaaa tattacggca attataattt taccgccagt gtgagcaata
    17641 gattcttcga taatcgcctt ggaataatag taaatgcaaa tacagataac tatgaccgta
    17701 gcgccgacaa attgcgagat aattggcgga attataatga aatgcttgct accggagaat
    17761 tatttatgat tgaggaaata gtaaatcgaa aacggaccgg ggcgagctta ttattagact
    17821 ataatctacc aaaagggaaa attaccgcta atggtttttt caatcagctt aattccgatg
    17881 gattaattcg taatagccag gtcaatacca tggaactcgg ttacgctact aatcgccatt
    17941 attatagagt tgaagaagaa aaaagtaaaa caaacgtttt taccagtgca attggaatta
    18001 atcaagactt tggatggttc cggtatgatg ccggagtaag ttactcgggg acgaatactg
    18061 atagtcctga tcgccgagta acagaatttg tacaagaaca aagtggttta ctgcccggat
    18121 atcccaagtt cgttgatccc aaggctctgg taccatattt taatgtcgat acaattaata
    18181 ctaatatggc ttctttatat agctatagta agaaaattac agagaacaca tcatccgcca
    18241 aacttaacct tgaaataccc tttcggtttg ggaatatttt gagtggctct tttaaaaccg
    18301 gcggaaaact gcgctggatt gatcgcagta atgatgaaaa tcaacacgga tatagtggat
    18361 tacaatacgg gtcaggccaa caaaatccgg tatttttaca tgttgataaa gtctatccag
    18421 aatggggcat tatggataac gttgaggaat atcagttgct aaatgtgttg ccatttttga
    18481 aagattactc tcgtcctgat ttcctgaatg gtgattttcc ccttggattg acttttgatg
    18541 aaaaaaagtt gcataagatg tccgatgctt tgatggcggc tccggatagt ctgaatttat
    18601 ggctgcccta ttccgttggg acattcggtt acgactatga tggttttgaa cgttaccaag
    18661 ccgcttattt tatgggtgaa tttaattttg gtagttatct cactttaata cccggagttc
    18721 gttatgaaaa ggattattca aaatatcatg gtcaacgcta catggttaac caatccggtc
    18781 agactgtgga gcaaccgcct tctgaatttg ttaggcttac aaaagagagg gagaatgaat
    18841 tctggttacc aatggtaaat ataatagcca agcctacgaa ttggttaaat ataagattag
    18901 caaggaccga aaccattgca cgcccggatt ttctaagata cgcaccgatc tcatatattt
    18961 cttcagatag aaaatcggta aatgcggcta attattcatt aagaccttcc caatcaacta
    19021 attatgatgt gggtgtttct ttgtttaaca atgagattgg tttactttca gttaccggtt
    19081 ttcataaaaa catcgaggat ctaatatttt attcagcggc tttttaccgt ccgggagttg
    19141 tattagattc ggctttggat attccccaaa gctggttaaa agccaacccc cagataaatg
    19201 cctatagaaa taatcctgat cctgccaaat attacggtta tgaaatagaa tggcagactc
    19261 acttctggta tttaccatct gtttttaagg gaattgtctt aaatattaat tatactcaca
    19321 tatattctga gatgaaatta gtatatgata gtctagtaac ccaacaagtt ggatcacggc
    19381 gagtctattc attggtgcca gcttatatca agactcgtat gcctgatcaa cctgcccatg
    19441 tttttaatat cacgattgga tatgatttaa aaggattctc agctcgcctc tcttatttat
    19501 tccagactga taaattaacc ggtattgggt atgatggcgt aattcccacg acgagattaa
    19561 gtagttatac tagtgattac gggcgctggg atctaacctt acagcaaaag ctttttcaaa
    19621 atattcaatt gtttgccaat tttaataatt tgaataatag gtacgaccaa agttgtatcg
    19681 gagcagattt aactcatccg agctatatcg aatattatgg atttactatg gatttaggta
    19741 ttagagtcaa tctttagagg aatatttaat gttgatttta ataaataggg ggaaatcaaa
    19801 tgatgttagc aattcaaatt cacccgcctt aagagcgtta tttaaactaa aaccattaaa
    19861 aaatgaaagg agaaagtgcc aaaaaaatga ttttaagaga aaaattgcta ttaaattgcc
    19921 aaaccaagaa aattaatcaa aaggaggatt atttaatgaa gtctaaaaac ttagtagttt
    19981 ggtttactat gctggcagtc gtgctgatta tgtcggcagg ccagatttta gcgcagactg
    20041 gagatacgct ggttgtgaat tgggatgacg gcgctggcaa tattgtccag gatgctcttt
    20101 acaatgccgt tataggagac accgtagctg gcggtgctcg tgccaatctg aatcgggtct
    20161 ataaactgaa agccggtggt tattactgga attataaaac cattagtaat gacttccctc
    20221 tgcgtttagt aggagaagta cccacaggtg acgaacatcc ggctgtaatt cagatggtta
    20281 cagatgcaga gagtggaagt acaccaggta aaatgatttc ctgtgccagt gatcttacac
    20341 tgaagaatct ttatatcatt ggttctgatg aactgggctc ttcagggtat tatcaaccaa
    20401 ttgaagttaa cgctgttgcg aaaaggttca ttattgacaa ttgtgtgttc gagcgttcaa
    20461 attttgcgat tgttgctttc gcaggcggca aggataatga cctctttatt accaactgtg
    20521 tttaccgcaa tttacaggaa gatgtgccaa cccaacagtg gtgcgggcgt ggactctcgg
    20581 tatggactga tattgataca gtagtcattg aaaataatac ctttttcaat gttaatatgt
    20641 gcgctatcca gatcgagaat ggtgccgcaa attatcttcg cttcaatcat aataccttgg
    20701 ttaatattgg tcgtgctttc acttcaacta ccaatgtctg gtggagagaa gcttacttca
    20761 cgaatttgct gtttgtcaat acctggtggc atggtgaagg ttattgtgat tatagtgtac
    20821 aatatcaacc aggtcgtgac ccaagggcat ataccacggg catctttaca attaatgata
    20881 tgccgtcgag atatgggacc aacaaagggc gtcgtatcct attttccaat acagccgcct
    20941 atctagatcc gtattttaca gaacattatg gagatacgat caggtttcag ccttatgtga
    21001 atgctatcac ggattcgttc tttactacat tcagtccggc caacggtggc cagatggtta
    21061 ttaaagatac gacctggctt agtacaaaac cagcattcac caatgatcct aatactccag
    21121 ctcaattaga ggcaatgtgg aatcacatca cggcctccag aggttatcag tattatggaa
    21181 ccggagtcca ggctaagcct tattattatg ctttgccgac tgatccggtc actggtgata
    21241 ccctttgggc agaaccaagc tggcctttac ctgagaattt cacttatagc aatgatctga
    21301 caacgcaaag taccgatggt ctgccgctgg gtgatttgcg ctggttccca gccgccaaag
    21361 cgacttttga agcgaataaa gattcctacg taaatgctat cgaatggatg ccgggtgaaa
    21421 gaatcataat aaaacctata tgggaagaac aagccgaagc cgggactgta agtggaactg
    21481 ccgctgttga accttttacc ggtgaggcgt ggtacacctt acaaggcggc acgagtgtcg
    21541 aatggacctt caattctact tatgccgggc atattgatat gaagatgaaa gccagagccg
    21601 atgggcaaaa cattggtttt gactttattc tcaatggcaa aaatatcgtt gatgccgctc
    21661 gtaagtgggg tcaatttgtg gtctggactg gttctggtga tccttcaacc ttctggaccg
    21721 gtaaaagtac cactgaattt tatgaagctt tctatgcttt tgctgatctg catagttttg
    21781 gcagtcttga cgagattggc gtagtagccg gtgaaaacac cctgaagcta caatattcct
    21841 ggaatccaat ttcctttcag tggattgagc tatatgaagc cgggacaaca aacttgattg
    21901 caagattagt accttcagcc gcggttaata atggtgctac tcccggtggc attggcgatt
    21961 gggtacctca gggatttaac tcggtcgcct taggaacaaa tggaacggtt actttcagtc
    22021 tacctaattt agacgctggt cgctatcagg tgcgggtgtt ctatcagaat gctggagcag
    22081 cctcaaccgg taaagtacta gttgatggtg cacaagcgac gaccttttct ttcgccagtc
    22141 aaccggctgg taccggatta gacgtcataa ctgatctttt tgatttgacg gctggtaccc
    22201 ataccttggc tatttccggt agtggtgcaa aagttgactg gatacaatta ctcaatgaat
    22261 ccagaatttc gggtatcaag aaggtaactg ttctggatgg attctcgctt tcgcagaact
    22321 atccgaatcc gttcaacccg actactatga ttcgctttga gttgggcaaa ccttctaatg
    22381 tcagattgac agtctataac ctgttaggtc aaagagtcgc tacattggtc aatggtcata
    22441 tgagccaggg tgctcatatt attcagtttg atgccagtca tttggcgtca ggcatttatt
    22501 tctatgcgat tcaagccggt gaatttaaag aagtaagacg gatgttgctc cttaaatagg
    22561 actgcgaccg attagttgga atgaatagag tagccggcag gatgaaaaaa tacctgccgg
    22621 ctctcttttt tcggatgcag ttagtgaatt aggaaagttt atgaatagac gtgaatttaa
    22681 taaaatgttg ctttgtgcgg tcggttcgct ggcactacct aaaattgacc tcttcggttc
    22741 taaacttgaa caagcaccgc cactgcattt tccacgctat cgaggattta accttctggc
    22801 taaattcagt aactgggaac ccagaagtaa attcgacgaa gaagatttcg agattatggc
    22861 ggagttggga ttcgattttg cgcgaattcc gatgtcctac tggtgctggt cttctaaaga
    22921 tgactggtac cgcattgatg aagatgtttt aaaagatata gacgaagtaa tcgagttcgg
    22981 gaagcagtac aatattcaca ttaatctgaa tttgcatcgc attccggggt attgcattaa
    23041 tggacgcgat caggaaccga tggacctcta cgaagatacg cctgaaagaa tgcaaaaggc
    23101 actggacgcg gctatttttc actggaaaat gtttgctaag cgctataaag gcatcccgaa
    23161 ttctcagctt agttttgatt taattaatga accgccgcat accaaagaag aaacccgtca
    23221 cgtagaagtc gtcacggctt tggttaatgg gattcgcgag gaagacccgc atcggttgat
    23281 cgtagctgat gggaaagacg ttggtcgcac gccgatcttt ggcattgctg atttaggagt
    23341 tgttcaaagt acgcgcggct atgatccaat gagcgtcagc cattataaag cgaattgggt
    23401 accgaaagaa tcctatcaga ctttcaagaa accaacctgg ccgcttaaag gcgatgatgg
    23461 caccatctgg gataaagcaa tgctgaaatc tcagttaatt gatccctgga tgccattagt
    23521 agcaaaggga gttccagttc acgtcggaga gtggggctgt tataatcaaa cgccgcatga
    23581 tgtagctctg cgctggatgc gtgatttgtt atcactgtgg caagaagtcg gctgggggta
    23641 cgctctttgg aatctaaaag gtgattttgg gattctcgat agtaaacgca aagatgtgaa
    23701 atacgagaaa tataaaggac atgatctcga tcgcaagatg ctggagctct taaaggaatt
    23761 ttgagtgcgc tatggaggct gtaagattcc aataattaaa aacttgaaaa tcaggaatga
    23821 tataaaaact cggtccatta tattgcttat aattatcaga aataaatgag gaattaatcc
    23881 tacctgccga ttgtattctg gatatttctc aaaaaaaggt cctagcagat caaggaatgg
    23941 tttagaagaa atagtcaaat ctaaaaggag aatcaaaaga tgagtccgct atataagatt
    24001 atgttagttt ttatcgtttt tttagcaatt gtgcaaccat cgcgagctaa ggaaaactac
    24061 aagaatttca aattggcgat ctactgtccg gcgcgcgatg tgcaaagaat ggctgattta
    24121 aattggctgg aaaagaactt caacctnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24241 nnnnnngagc aagcggcgga agcctctcaa aaactgatta ttgacccggc gaagaaggtc
    24301 aatcctaagg taaaaataat catcaagtat ccgaactggt acgatcattt tcaatactgt
    24361 ggatttaatc tggaaaagca gccgactcaa tttgatctga tctatactgg cacggaaacc
    24421 agagacccgg tttatactca tcagcattta caagcctatg agagttatgc cattatgcgc
    24481 tattttgaaa atattgcgcc cggacggaat ggtggcggct gggtggaccc ttttaatcgt
    24541 cgcacactcg atcgttatgc ggaacaaatc aacatgacat tgtttgccaa agcccgagaa
    24601 ttaacccttt tctgcttcgg agcattgctg gaagcaattc ctcaggaaga tggctcgttc
    24661 agacctttga gtccggttgc gccagtcgcc ggatataacc tggataagat agactgtttt
    24721 ctagggaaat taggtcagcc gttcggcatt aaaacctata agccttacca ttcctccggt
    24781 gaagatttcc ttcccaatta cattggtatg ctgggaattc cgatggaaat tactcctgat
    24841 tttccggcga atgatgagat tattttcctg accgaacagg ctagcttcga tgctaagatt
    24901 gttgagaaaa tcaagaatca actgattaaa ggtaaagacg tggtcatcac ttccggactt
    24961 tatcgtgcgc tccaggggaa aggcatcgag gatattgtcg aattagagtg cactgataag
    25021 aaagcgttag tgcatagatt ttacgactgg cagaatgtct ttatagccga aaatgatatt
    25081 ctgattcctc aaattcgcta tgctaccaat gattcctggg aactgattac cgcgctcgat
    25141 agcggcaacg gctatccaat cttccatcaa gccagttacg ctaacggcaa actttatgtg
    25201 ctgactattc cggataactt cagcgaattg tacgaattac cgcgcgaagt tttgacaatc
    25261 attaaaaggg taatgatgaa aaatttcttc gtttataccg atagtcccag cagtgtgagc
    25321 ttattggtct atgacaacaa tacctttatc atttattcct tcaaagaaca taatacggat
    25381 gtaaatattg tcctggataa taaattcagt aaagtcactg atcttcttgc caatcgagaa
    25441 ttaaccggct ataaagaaaa caacaaatta gtgatcaaga ctgcaatcgg gccgcattct
    25501 tatcgggtgt tcagagctga ataatgatta tcaataaaaa ttaatcaata ggagaatgtt
    25561 atgatgagaa taccgtacat ttttggcatt accgttttac taatttttat ggtttcctgc
    25621 caatctggat caaaggcggt ggatgccttt gagcaaaatg aaaagcttgg cagaggagta
    25681 aatatcttag gctacgatcc gatctgggat tcgttcgaga aggcgcgttt caaggagcac
    25741 cattttaaac tcattcggga ggcaggcttc aacaccgtcc gaattaattt gcatcctttt
    25801 cggcatatgg atccgacgac ctttgaatta cgagaacatt ggtggaaagt tctggattgg
    25861 gcggtggaaa atgcgctggc taatggctta atgattattc tcgatatgca cgaatattat
    25921 gctatggctg acgatccagt cgctcgtaag gatatgtggc tggcattctg gaagcaggtt
    25981 gccgaacgat accaaaacag ttccgaaaat gtgctctttg aattattaaa tgaaccaaat
    26041 ggcaaactta acagtgaatt gtggaatggt tttctattgg aaggtttagc ggtggttcgc
    26101 aattccaatc cccatcggac ggtcatcatt ggtccggcac aatacaacca aatcaatcag
    26161 ttagatagtt taatattgcc ggaaaaggat cgtaatatca tcgtaactat tcattattat
    26221 tcgccgatgg cgtttaccca tcaaggtgca tcctgggctg gtcgaactga ttcgctgggt
    26281 gtccaatggt tgggcacagc cgaggagaag caagccattc tggacgattt cgccaaagct
    26341 caaaactggt caaaaaaaca caaccgcccc ttgtttctcg gcgagtttgg tgtctatgat
    26401 aaagccgata tggaatctcg aactaaatat ctttcgtttg tgaccagcac ggtggaagat
    26461 ttaggatgga gttgggcata ctggcagttt gacagtgatt ttatccttta tgatattgat
    26521 aatgaacatt ggattgagcc ggttttgaaa gccttgatac ccaaaactgc cggcgttaat
    26581 tgaggtattg gaatggcaat aatcatttgg agaagtataa agtaggggca gatgataact
    26641 tgaataaata tcgtcactta ataatttact gcataattat aataatcttg ataggatgtg
    26701 ctacacaaat gaattcagat aaagaaatgt tattaaaaga acttgaattt gctctaaaaa
    26761 atgaattttt agatgtctgg tatccagcgg ctctcgatac agtttatggt ggttttctga
    26821 gtgactttac gtttgactgg cggcctgagg gtccgcagaa taagatgatt gtaactcaga
    26881 gtcggcaagt ctggacggct tcgcaggcgg caatgtttta tcaagatgat caatatcgca
    26941 aaattgccga acatggattt aattttctaa gagataaaat gtgggataag acctatggcg
    27001 gcttttatca attgcgggat cgtcagggga atttaattcc ctatggagat ggtcttcata
    27061 aagtggctta tggcaatgct ttcgcaatct atgcgctggc tacttattat gaagcttttg
    27121 gcgatacttc tgctcttgat ttggcgcgcc aaacttttgc ctggctcgaa aaacataatc
    27181 atgatcccca gtacaaaggc tatattgatg ccaccaatct ggacggttcc tggataatgt
    27241 ggcatccaac tggtaaaacc aatcgtaata taaccgagac ggttttgaag gactataatt
    27301 cttcaattca cctgctggag gcatttactc agctctacaa agtatggccg gatagtctcg
    27361 tgcgcgaacg cctactggaa atgctgactc tggtcaggga cacttttgtc aataaaaaag
    27421 gttatctgaa tatgtatttt acgagagact ggcaaccact atcggtgtat gacttttccg
    27481 ccgagccgca ggaagccgac tatttttttg atcatgtttc tttcggtcat gacgtcgaga
    27541 ctgcctattt gcttttagaa gcggaacata ttctcggtaa aaaatcctat tcggtaactt
    27601 tgccagttgc ccgagcgctg gttgaccatg ctctcgagaa cggctgggat caagacaatg
    27661 gcggattgta ttatcagggc tactacttcg acggttcgga tacgattacg atttttgatg
    27721 ataccaagga atggtgggtt caagccgaag ccttaaatgc atttttattg atggctaaac
    27781 ttttcccgca agatcagaaa tactacgatg ccttccggaa acagtgggaa tacatccaac
    27841 gctaccttat tgatcaccag cacggcggct ggtattcaaa aggtctggat aaaagtcccg
    27901 agcgcgccaa aggtcctaaa ggatacgact ggaaagtcaa ttatcatgaa acccgcgctt
    27961 taataaactg tatccagatg ttaaaagatg aacatgaact attaaagtaa cttaaggatg
    28021 gattaattat gaagaaatta tttcgatttt atattgtttt gataattatt gtatccttgg
    28081 ctttttctca agaaaaaaat aaagtagatt tgtcaactgg ggcaaatttt atcgcaacga
    28141 aaacttacgc gccggaagaa gacgcccgta tcctgaaact cttcgaagga ttgcgcgtgg
    28201 ctgatgtctc ggatggaatg gatatggtag gcttgccggg cgtcggactg gttgatccgg
    28261 cgattcatgc tgattggctt gatctggaag acctatcgca tcaaattcgt ggcattgccg
    28321 tcacggcgcg ctatgttccc acgcaacgtc ctgaccgacc gcaagccggt gaaaaatttc
    28381 aggaatggga agggaatttt tataatgttt attcgcctga aacttttgcc gagttgattc
    28441 gacccggctc ggtaattgtg ctcgacgatg ttgaggaaaa ggacattggc accataggct
    28501 cttacaatat cttagcttgg cataaaagtg gggcggtcgg agttgtcacc gatgcctgtg
    28561 cgcgtgatac tgatgaaatt gccaaggaga aagtgccttt atatttaaga aaaaagggtc
    28621 gcggcatcag accagggcgt aatgagctgg aatcggttaa tcgtccggtt gtcattggcg
    28681 gagttttggt ttgtccgggc gacgtagtgg tggcggatgg tgacggtgtg atcgtagtcc
    28741 ctcgcaatgt tgctgagcaa gttgccagat atgcgcggga aattttggtg aaagataaag
    28801 ctagtcgaaa ggatttatat aaaagtcttg gtcggcctct ggataaaact gtcgaataaa
    28861 atgccaaaat ttaccagtaa gtgaaatagt ataatgtaaa tcgggaattt ttatgcgatt
    28921 aaaattaatt gatatcgtta taattctgct atacttatcc tttaccgttg tcatcggcct
    28981 ggttcttaaa aagcgggcac aggaaaataa aaccgcgtat ttattaggcg gcaataaatt
    29041 gccttggtat atgcttggaa tatcgaatgc atccggtatg tttgatattt ccgggacaat
    29101 gtggctggtg acactgatgt tcgtttatgg gcttaaatca atctggattc cctggctctg
    29161 gccggttttt aatcagatat ttttaatggt atatttgtca gcctggctaa ggcgctcaaa
    29221 tgtcacgaca ggcgcagaat ggattggaac gcgttttggt tctggccgag atgcgaccct
    29281 ttcccataat atcgtagtag ttttcgcttt actgagttgt ctcggatttt tagcctacgg
    29341 ctttatcggt ttgggaaaat ttatggaaat ttttatcccg tgggaagtag tatccaatta
    29401 tgtgcctttt agcgttccag acaaatttgt cccccatttg tacgggattg tttttacggc
    29461 gttcagtgtt ttttatacca ttctgggtgg aatggtcagt atcgtctggg cagatgtgat
    29521 tcagtattgt attatgggag tctctgcaat cgtaattgga ataattgcta tgcgggcgct
    29581 ttcgatccat cccctgatag taccggaagg ctggatgagt ccctttttcg gttgggaatt
    29641 gaatatggat tggagtggca ttattgccgg agtcaatgat aagatcgcca gcgacggcta
    29701 ctcgcttttt atgattttct ttatgatgat gctgttcaaa ggtattttag tcagcgcggc
    29761 tggcccggcg ccgaattacg atatgcagaa aattttggcg actcgctcgc ccaaagaagc
    29821 tggaatgatg agcggttttg tttcggtgat cctgaatccg attcgttata taatgattac
    29881 tggatttgcc gtgttgggca ttatttatta cgatcgtttg gatttaattg tcggtggacg
    29941 tctggatttc gagcaaattt taccttcggc gatcagtgaa tttgttccga ttggttttac
    30001 tggtctgatg ctggcgggac tattggcggc atttattggt acttttgctg gaacgctgaa
    30061 cgccgcgcag gcttacattg ttaatgatat ttacctgaaa tatatgcgtc ctaaagccac
    30121 caaccgccag gtgtcatcgg taacttatat atcgggaatt ggtatcgtcg tcattagcat
    30181 catttttgga atgtttgctc tggacatcaa tagtattttg caatggattg tttcggcttt
    30241 atgggggagt tatgtcgttt ccaatgtttt gaagtggtac tggtggcgat tcaacggcta
    30301 tggttatttc tggggaatgg tagctgggat tattccggcg ttaatttttc ctcagatttt
    30361 cgacacgctg gatttgtatt attttccatt gattttattg atttcgctgg cgggggcgct
    30421 aatcggaact tatcgccatc caccaacgga tgaagcggtc ttgaaaaaat tttataaaac
    30481 cgtgcgtccc tggggattct ggaaacccat tcatgagaaa gtagttgctg aagatcccaa
    30541 ttttgtcaat cgaagttcct ggaagcggga tttactgaat gtactgatcg gaattatctg
    30601 gcagacgacc ttagtggctt tgccaattta ttttgtgctg aaaaagtttg gtaatgccgg
    30661 gatttgttta ttgataatta tagtaacctc catctttttg aagaaaaatt ggtacgataa
    30721 aatggaagat gattaatttt gaagaaatct ttataattgg caaatcgaga tagaatatga
    30781 aaaatgatgc aattgcaaat tggcgtcagc aggttagtcg caatttatac gaaaatataa
    30841 ttccgttctg gatgacatat tcaattgatc gtcggaatgg tggattttat ggtcggatta
    30901 ccaacgattt acaggtggat aaaaccgcac cgcgcagtgt ggtgctgatt gctcggatat
    30961 tatggacttt ctcggcgctt tttagatttg ataaggatac ccggcatctt gaaatggctc
    31021 accgggcgta taattatctg actaaatatt tcagcgatag taaatatggt ggagtttact
    31081 ggttagtggc gcctggtggc gaagtccttg aagataagaa aaaaatttat ggacaggctt
    31141 ttgccattta tggtctcagt gaatattatg cggcttccgg tgaaaaagcg gctcttgaaa
    31201 tggcgcacca gatttttcag ttgattgaaa aatattgtca tgatgcgatt aatggaggtt
    31261 attatgaagc cgctaatcgt gactggtcgg agactcaaga tctgcggcta agcgcagttg
    31321 atttaaacga aaaaaaatca atgaattctc accttcacct actggaagct ttgactaatc
    31381 tcctgcgggt cagttccgat agtttagtgc gggctcgtct gcaggaattg gtagaatgcc
    31441 acctgagata cattctcaat ccggtgagta atcatttata tttatttttc gatgaaaaat
    31501 ggaacccccg cttgaatggc gtttcatttg ggcatgatat cgaagccagc tggttaatca
    31561 cagaatccgc cgaagtcctt ggtgatgata aaattcaggc gcgggttaaa tcagccgtcc
    31621 ataaaattgt agcggcggta attgccgaag gctttaatca gcataatgct ctacctgtgg
    31681 ctaatgatat aaccgggagt ttatcagtca ataactttga gtggtggcaa caagcggaag
    31741 cgcttgtcgg tctgattaat gcctaccagc tctatggtga ttcatcctat ttggatagag
    31801 cctgccgcct ctgggatttc attgataaat attttgtgga ttggcaacga ggcgaatggt
    31861 attatgaaat ttccgccgaa gggattccga atcccgctaa gcctaaggtc agcgaatgga
    31921 aatgttgtta ccataatacc cgtgcttgct tggaagtcct aaaacgcact gctcaactca
    31981 ttaatcgaag agaataacaa attgaagaaa cccagtaaaa atgaattcaa gaacagttta
    32041 aataaattac taaccgatca agacgcttgg ctcaatttgc ctaatcaggc gtgtccttcg
    32101 caaaatggga tttatcaaag gtatcagcat ccggtcctaa ccgctaaaca tattccgatt
    32161 aactggcgat tcgatctgaa ttatgcaact aatccatttc taatggagcg tttaggagtc
    32221 aatgctgttt ttaattgcgg cgcaatcaag ttcaacgaca aatattgtct ggtagcgcgg
    32281 gtggagggac tcgaccgcaa gtcgtttttc gcagttgccg agagtccgaa cggcgttgac
    32341 cattttaaat tctgggaata tccgataatt atgcccgaaa ctgaaattcc ggacacgaat
    32401 gtctatgata tgcggctgac ccatcacgcc gacggctgga tttatggtgt gttctgtacg
    32461 gagcgcaagg atcccaacgt tccggagtat gatacttcta cggcggtggc gcaagccgga
    32521 attgctcgca ctcatgattt gaaaaaatgg gaacggcttc cggatttgca aacgaagtcg
    32581 agacagcaga gaaatgtggt tctacatccg gaatttttcg acggcaaata tgctttttac
    32641 acgcgaccac aggatgattt tattctggcg ggaacaggcg gcgggattgc ctggggactg
    32701 gctgataata tcgagaaagc aattattgac gaagaaatca ttattgacag tcgagtttat
    32761 catacgatca acgaagtgaa aaatggcgag ggaccggcgc cgctcaaaac cgccgaaggt
    32821 tggatacatc tggcgcatgg tgttcgcaat accgcggccg gtttgcgtta cgtcttgtat
    32881 atgtttctga cggatttgaa tgaaccctgg cggataatcc ataaaccagg tggttatttt
    32941 ctggctccgg aaggagagga gcgtatcgga gatgtctcca atgtagtttt tagtaacgga
    33001 tggatcgccg atccgaacgg gcgcatattc atttattacg gatcatcgga tacccgaact
    33061 cacgttgtga tgaccacgat tgaaaggctt ctggattatg tgaaaaatac tccggaagac
    33121 ggacttagat cggcaaaatg tgtcgaacaa agagtggcac taatcaatca caattggaat
    33181 atcattgata actttaaaaa gtaattgtta gttctatgaa cattaagttt taatcgggga
    33241 atcttggagg gtattatcat gaagaaatcg attttgatta taatgctatc aacgatatcg
    33301 ctatttggac agatccccgg ttttacggat gattttgaag acggttcgct ggatacattg
    33361 tggaatgatt ctctaatgac tctctggcat agcgactggt atccggatat tttcaatctg
    33421 acagagagcg agggagttct cgaggtggct tatgaccgga aatcaactga tagcgactgg
    33481 ccggcatttt attttacgcc gccggaagtc atcaacgtcg ctcataatcc gaaaatcgtt
    33541 atcgagatca aatccgatat cacgattaac tttgcgatta aaccggttta cacgaacggc
    33601 aaagataatt accagtcaac taccgttccg ggttcgaata cgtggcaaac ttatactttt
    33661 acgctgagta attatgccgg tggttattgc aatcaggtcg tgttccattt tgacggcggg
    33721 acgaaagttg ataagcaggg aacagtctgt tttgataatt ttattatcgc cggattttca
    33781 atcagtatcc aaggactaaa tgccaaattc gatccgatca accagtatgt tgatttagtc
    33841 tggaactgcg ataatgaagc ggctgttgat cattataatg tgtatcgcga tactctcaaa
    33901 ggcttttcct gtacgactga aaatttaata ggcgaaccgg aagggaaaaa ctggcgcgat
    33961 acgcaaatag gtatcggtac ttattactat aaagttgcgg cggttgatac gatgggcaaa
    34021 caacacgcgg tttcaatgga aatggcttgc cgaacctata cgatcggagc aattccggtg
    34081 gtcgaaatcg ccgatgtcaa ttcgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    34201 nnnnngaatc cgattatcac ggctgtttaa atgtatcttc ggttaatccg cattatctga
    34261 tttatcacga tggcacaccg ttctatggaa ttggcgctta ctatccctgg ggcgtgcgca
    34321 atgacgacga tgggctagga attttggccg catcaggtgg caacctgttc ggttactgga
    34381 acggcaatta cgatggagcc ggtaacggcg gtggaattta tcaaatcgaa tcaatacaaa
    34441 gtggcatcgg ttattatgat caacgtaaat gcgctcgaat tgatgaaatt ttagattggg
    34501 cggaacagcg taatttagaa atgatgcttg ccatctggcc gcacgatatt ctcgatcaaa
    34561 gcacctgggg ttataagggt tgggatgata atgcctataa aactgtctgt aacgccgcta
    34621 atttttatac cgattcgctc gcctgggtat atcagcagaa attgtatcgc tatatgcttg
    34681 ctcgctgggg ttactcgcgc agtctcggta tctgggaatt ggtgaatgag attgacggta
    34741 ctgacggctg gtcacgtggt aataaaaccg gagcgctcga atgggttcag aaagtccaca
    34801 attatctgaa ggaaaatgat tattttaaac gacctacgac tatctccaag agcggaagtt
    34861 ctgcgaatta ctggcctgaa ggttatcaaa tttgtgattt gcccaatgtt cacctctacg
    34921 aaaaaggatg gagtgcgcct tattccagcg atccaatccg aagtagttat tggacatatc
    34981 gcaatgtcgc ccgtcaatta tggaccggtt tcgagaaacc gggaattttc ggtgaagccg
    35041 gtgcggaacc gaccaatatg tatgccgcaa atattgaaaa gggttccagt aaatatgtcg
    35101 aaatttatca taatgcgctc tggtcagcct gggcgagtgg tttggcggcc acgcccatct
    35161 ggtgggaaat gaatgatcga gagctaatga ctgacgaggt tttcgctcag atgaaagcct
    35221 tcgcgagagt tgcttctgac attgactatg ctgacttgaa cctgatttca agtaatgcga
    35281 gtgccgatac cgcggatgct tttatgatga cggcggattc cctcgggttc ggctggtttc
    35341 gatcatataa taatagtgcg atttccggtc gttttacacg gattagtggc ttgaaaaccg
    35401 gaacatatca agctatctgg tatgatacct gggccggcga tagcgtcatt gttggtaatt
    35461 gtgtatcggc tggtaatatg atctatactg aaattcctga gatgccgact ccgtgcaaag
    35521 atattgcctt acggttgacg aaaaccgaag atgggactca accggcgnnn nnnnnnnnnn
    35581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35641 nnnnnnnnnn nnnnnnnnnn nnnnnnntca accgaaggat tagccagcgt ttctctattc
    35701 aataatgtgt tgagcgagat cgaagtcgaa aaaaatgctt ccattccgac agaatttgct
    35761 ctgaaagcta actatcctaa tccctttaac gccagcaccg tgatcgaatt ttccctgccg
    35821 aaaactacga acataaagat tgatgtctat aatctaacag gacaattagt cgagacactt
    35881 gtccgaggac ggcaatcggc tggaaattac aaaatattct ggcatccggc taatctgtcg
    35941 tcaggtatct atctgtacca aattacctgt ccggaattca cggctactag gaagtgcatg
    36001 atcctgaaat aggagaaagc gacttaaact aatttatatt tttctatttt tttaattgtt
    36061 tatataatct atttatttat atagatatac ttaaagttta ctaaatctat atttatttaa
    36121 aatattttcc tcaaataatt ttaattgact tttaacttct aaggtgttat attaaaacgt
    36181 ttcgatatta tttatgagaa aatatgagta caattaaaga catctcaaaa aaagctgggg
    36241 tttccatctc gactgtttcg cttgtcttga ataacaaagg ttatgttaaa ccagaaacct
    36301 accagcgggt tcttagtgtg atgcatgaac ttaattacaa accctcgcgg tcggcgcgta
    36361 agctagctac ccgcaaaaat ggtaatatcg gatttatcat ttgggaaagt catttttacg
    36421 aagttgaaat gttttacagc caggtttttc taggtatgga atatgccgca cgtaaaagtg
    36481 acagctatat acttttaacg accgtcaaag atcaatttga tcctaaaact gatctacctc
    36541 gttttttaca gtgcaatgat gtggatggtg ttgccttagc cggtcgggta ccgcacgaat
    36601 tggtttatta tttagaaaaa gaaaggattc cgtttgtttt aattgattat tcaattccag
    36661 gtaaaaagta taacagtgtc cagattgata attacaatgg tgcttattca gctgttcagc
    36721 atttagtcga attagggcga aaaaggattg gtttcgtcgg aggtactttt tttcattcat
    36781 ccattaaaga gcgctatcgt ggttataaag attgcattga aaccaataag gtaatgactg
    36841 ctgatgatat ctctaagtat acctacgtcg aagacactga aacttcgcct gccattggag
    36901 aaaagggaat tagcagtcta ttaaaaaacg atgctaaact ggatgcggta ttttgttgta
    36961 atgacccaac tgctatgggt gttatcaaag aaattcaaag aaggcaactt aaaatcccgg
    37021 aagacattgc tgttgttggt ttcgatgata ttcctaattc ctcttactct acaccaaaat
    37081 taacaaccgt acatgttccc aaattagcgt taggtaagga ggcttttaat ttactcaata
    37141 aagttattga acatcccctg gaaccgccgc agacgcgagt gattgcaacg acccttgtga
    37201 agcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntggaac ggttacaata
    37321 ttttctaagt atatgcgaag aaaatgaatt atatacttta ccgactttta ttgttggaca
    37381 tatgtcgggt gaggattggg gcatgccatg gttaaaaggg agtgaccttc ttaaagatag
    37441 gcgtgttgtt gacgcaatta agttttatat caccacggta attaaggcga taaacaattt
    37501 caagtatatt caagcctggc tactatctaa tgaattacct aatttcgttg gtaaacagga
    37561 gcctgatgat gtaactgcgt gggttgaaga aattatcgca acgattcggg caattgattc
    37621 gcaacgacct atcagtattg gagatggtgc ctgggcacca gaaatcatcg ccgatcaaac
    37681 tggatttcat ctaaggaaat tgaatttata tcaggatttc gttggattac atttctatcc
    37741 acgcggtatg agtccatggc accattctta tacgactgcg ttccgtatcc agttggcaaa
    37801 agtttggggc aagccagttc ttgtagaaga atttggcact tctactactt tgtgttcgga
    37861 aataaatcaa gctaattatt atcggaatgt attttatagt gctttaataa atggagcaca
    37921 gggtgtttta aactggtgcc taaatgattt tgattttaaa gataaaagac catattctca
    37981 ccatcttttt gaagaacgtt ttgggattat cagaactgat caaagtctga aaccagcggc
    38041 ttatgaattc cagaaattgg aaaagattac tggcgatctg ataacaggaa aatataataa
    38101 agtcgagtat aattctggac ttttcatacc atcaagttat tattacaact acccatatat
    38161 gtttaaagta gagggtgaaa atcattacga tttttttctg gaatgtttct cactgctgaa
    38221 acgggctaat ctggatgtcc aaatggtagt agaaccggct caggaactta aaaatgacgg
    38281 ttgctattcg catgccctcg atctaaaacc tgaagattta ccggtgctat ttctaccggg
    38341 tttaagattt atgacaaaac aaactagaat tgccctggaa aattatgtcc gagatggtgg
    38401 ggtaatatat tttagttttg ccaatgatag ttgggtgact gactgggata aattggctgg
    38461 ggtggttacc gattgcaagt ttggagttcc tgactttaga gttaaaactg atttaactat
    38521 taaggtagaa gatgagtggg gaaaattcgc caaaaatgaa atatttacta tacctctttt
    38581 aaataatcaa ccggctttaa gttattgtcc aataataagt acgacagcta gagtcattat
    38641 gactgatgaa tatggaatgc cttttctgct tgaaaatgcg attggaaaag gaaaagttta
    38701 tttcacatca tatccacttg aagcgttagc tttagtaatc aatgatgatc accttagggc
    38761 aattctttgc cgtatataca aatctattaa ccggcttcac ggtaaacgag cggaaataac
    38821 gattgaaggt gatggactag aattaggggt ttgggagaat caggcagaat atcgggtgat
    38881 agtatttaat cattcttggc agaggcaaaa cggagccctt tatatagtcc acacgcctct
    38941 atcaatttct tctgatgaat ctgatctcag gacagaagag gaaggacttt attatttgac
    39001 gttgccccgc aagggagtgg cttatttaaa aatacaaaat gagttaaaat cacataaaaa
    39061 caaacaacaa acataggagg aaaaaatgaa aaagacttgt ttgttaacca tggttgtcgg
    39121 cttgatccta ttaaacttcg catttggcga taatttgata gggaatggta gttttgagga
    39181 tcagagtccg gcgttttgga gtcctttaaa cgggacattt ggggaagccg tagatatttc
    39241 atctacggag cattttgctg gttttcattc ttttgagatt acgaagagtg cggccagttc
    39301 ggatgtagtt ggatgggtgt ctgataacaa tgcgacattg tactggaaca atgcgacggc
    39361 cgggacatat gccttgaccg gatatgtcaa gacggtcggt gtgaatacga atccggcaag
    39421 tgatgctgag aaggttggga tagtatacga gttcaagaac gcatccggcg gtgagttgac
    39481 gaccgagaca gtttgggcag atcagagtgt tgcggataag gactggacca aattgacagg
    39541 ggtggcaata ttgacggaag cgccggcgtc agtggtggtt aaattggtaa tgggtaaaga
    39601 ggcgactggg acagtttatt ttgataatat agactgtaat acgacggaca cctggacgat
    39661 gggtatattc aattatggtg ctgaagatgt taatggctgg atggattggt attctgaaaa
    39721 tggtagttat gcggtggtaa ctgatgctga agctcatacg gggagttatt cggtagtaat
    39781 ggaacagccg gatacctgtt cgcagttgaa tgagttggta tattattcca agccttatcc
    39841 ggtagaggcg ggtgaatggt ataagatagg cgtatgggtt aagacagaga atgtactgga
    39901 ttcgtctgaa tttgagccga cttatattac gaaggaaggt atacctgaga ggataagttt
    39961 atgttatttc tttcatggag atgccgatat tgagacggct tgggataagt tgccgggtgg
    40021 tgataagttt gtatatatag atcagcgaga ggcaagtact ggttggactc attatgtggt
    40081 agctgagcag gcaccggcgg gagatgagcc tgcgacaggg atttcggttc gtgctcgatt
    40141 caataataaa gtgactggta aagcctggtt cgatgacttc accgtcgaaa aaattatcat
    40201 tgatggcgat aatttgatag ggaatggtag ttttgaggat cagagtccgg cgttttggag
    40261 tcctttaaac gggacatttg gggaagccgt agatatttca tctacggagc attttgctgg
    40321 ttttcattct tttgagatta cgaagagtgc ggccagttcg gatgtagttg gatgggtgtc
    40381 tgataacaat gcgacattgt actggaacaa tgcgacggcc gggacatatg ccttgaccgg
    40441 atatgtcaag acggtcggtg tgaatacgaa tccggcaagt gatgctgaga aggttgggat
    40501 agtatacgag ttcaagaacg catccggcgg tgagttgacg accgagacag tttgggcaga
    40561 tcagagtgtt gcggataagg actggaccaa attgacaggg gtggcaatat tgacggaagc
    40621 gccggcgtca gtggtggtta aattggtaat gggtaaagag gcgactggga cagtttattt
    40681 tgataatata gactgtaata cgacggacac ctggacgatg ggtatattca attatggtgc
    40741 tgaagatgtt aatggctgga tggattggta ttctgaaaat ggtagttatg cggtggtaac
    40801 tgatgctgaa gctcatacgg ggagttattc ggtagtaatg gaacagccgg atacctgttc
    40861 gcagttgaat gagttggtat attattccaa gccttatccg gtagaggcgg gtgaatggta
    40921 taagataggc gtatgggtta agacagagaa tgtactggat tcgtctgaat ttgagccgac
    40981 ttatattacg aaggaaggta tacctgagag gataagttta tgttatttct ttcatggaga
    41041 tgccgatatt gagacggctt gggataagtt gccgggtggt gataagtttg tatatataga
    41101 tcagcgagag gcaagtactg gttggactca ttatgtggta gctgaacagg caccggcggg
    41161 agatgagcct gcgacaggga tttcggttcg tgctcgattt aataataaag tgactggtaa
    41221 agcctggttc gatgacttca ccgtcgaaaa aatgaaggtt gctcccggta ctggtatcat
    41281 tactgataaa ggatttggcg gtgatatccc tgtcaagtat gcaattatgg ataattaccc
    41341 caatccattt aacccggaaa caaggatcgt ttattcactt ccgcaatctg gtaaagttaa
    41401 tttgacggta tataatatac tggggcaaaa ggttcgtaat ctgaccgaca cagttcaaaa
    41461 tgctggaact tttaccttga cttgggatgc tcggaatgat ttcggccagg atgtaccgac
    41521 aggtatatat attgccgtat tgacaattaa taaccagagg gttacaagaa aaatggttct
    41581 catgcgataa gcaaagataa aaacctccga aggctatttc ccgccttcgg aggtttttaa
    41641 cctataaatc aggtaaggat tattatgaag cgaaccacaa tcttaataat tatacttgta
    41701 attttataca tttcgcttct ggaagcaggt accacgggaa agatcaaagg acgggttacg
    41761 gataaaatta gtggtgagcc gctagtcggt gttaatattg ttcttgaagg tacttcccaa
    41821 ggcgccgcaa cggatattga aggtaactat gttatactta atgtgttacc aggtaattat
    41881 tccgtaaaag ctagtatgat tgggtatcaa actgtcattc aaaaaaatgt ccaggtttct
    41941 gttgatttga ccacacctct tgattttgaa atggtttcaa caatcttgga aggtgaagaa
    42001 gtagtagtga ttgcccaaag gccgcttatc agaaaagatg ttacttcgac ttcgtttcgg
    42061 gttagctctg aacaaattga tcaattgcaa gttcaaaatt tgagtgaaat tgttgagctt
    42121 caggccgggg tagttcaggg gcactttaga ggaggaagag caggagaagt gatgtatatt
    42181 atcgatggag ttccgatgaa tgatgcttat gctggtgagc atttattcga tgttgaaagt
    42241 gatatgattc aagaagttga agtcatcagt ggtactttta atgctgaata cggtcaggct
    42301 atgtctggta tcgtcaatat tgtgactaaa gaagggcaag aatattattc aggcaaaatc
    42361 acattttact gtggagatca cttaagttca catactcata tttttatgaa tattgatgat
    42421 ataaatccat tggataatac taactatcaa tttaacctta gtggccctat tcctttattg
    42481 gggaaaaact tatcgttttc tctcctgggt cggacgaata ccgatgacgg ctggttatat
    42541 ggtcaacgga ttttccgccc ttcggatttt tgttattttt ctgatgatcc aaacaagcag
    42601 attatccagt caactggcga tagctctttt gtctcgatga attcatcaaa tatgcaaact
    42661 ctccagggaa aactcagttt taagctttct cgtatggata aaataaatta taccggtttc
    42721 tatgaaaaat cgaaataccg tagttatgac cgattattta aatataatcc ggacggcaat
    42781 tatcgccatg aaaatatgag ctatcaaaac tcattgcagt atactcatat gttcaatcct
    42841 aatacgttct taaccgcgaa ttattcaaac ttgtttacta cctatggaca atatgtttat
    42901 aaagataaat acgatccacg ttatgtacca attgaatacc taaatgccac taattccaat
    42961 ggtttttcta caggtggcat gcgaatgtgg catcatttta gggataatcg tactgagatt
    43021 atcaaggctg atcttcgtag tcaaattacc gagcatcaaa atatcggtgc tggagtaagt
    43081 tataaaaagg ttcatttatc tcttcacgaa taccagattt attttgatga aaatgataat
    43141 attcaaatac cttcagattc ttcttgttac aataattcat atctacataa gccatatgag
    43201 atttcagcct acctacagga taaaattgaa tttggcgata tgataattaa tgttggcttg
    43261 aggtatgatt attttgaacc agatggtgtc gtccccactc aattttacaa tacagtagga
    43321 gctcccaaac gtaaagctaa atcttcatat caatttagtc ctcggattgg gattgcttac
    43381 ccgatttcag atcaaggcgt aatacatttt tcttacgggc atttttttca gataccgaat
    43441 tatgagtatc tttatgctaa tcctgatttt gaggtttcgc ttgttcaatt agaaggaagt
    43501 gaacctccca gcaatggctt caatgtgatg ggcaatgccg agttaaaacc tgagaaaaca
    43561 gtcagctatg aggtcggttt gaaacaagct attacagata acttaacaat cgaattgact
    43621 gaatataata aagatattcg ggatttagtc ggacagctta ctatgaccga tatctatggt
    43681 ggaaaatttt ggcgctacat taatcgcgat tatgccaatg ttaaaggaat cactatggca
    43741 atcgaaatgt tagaaaaacc cggaggaata ggcttttctg ttgactatac ctaccagtca
    43801 gcgaccggca atgcttcgga tccgttagat gaatatagga atcagggatc cgatccaccc
    43861 attgaatctg agaaaaaacg tcagccgctt aactgggatc aaactcattc attaaattta
    43921 actactacaa ctacccagaa gggatttcat atttgcctca ttgggaaaat cggtagcggc
    43981 acaccctata cccgcgaatc tccatattac aataatcgaa ttctaaatgg tgaacgcaaa
    44041 ccaatgacaa tcactttcaa tttgaaccta tctaaagata ttccttttaa gaatttgatg
    44101 attagcccat ttataaggat tacaaattta tttgaccgcc tgaataatct cgaagtttat
    44161 ccttccagcg gaacagccga ttataattac agcatgattt tcgaaaatta tcgcggttat
    44221 aaaacccaag aggagtggaa tatccagccg aactggtatg aagaaccacg caacgtaatt
    44281 attggttgct ctattagttt tgatcaaaaa aagtgaagtg ttcaaatatg aataagaaat
    44341 tatttttcaa atctctctta attataatgc tatttctcgg agtgactgct ttagcgcaaa
    44401 ccccctctag cttacgggga catcgaatgt atcggcgtcg gggtttgatg aatggaaatc
    44461 tcgtttcaac cctattttgg aattattgtg aggtaggaga ctatccggat gagccttccg
    44521 gttgctggcc gagtgccgaa cgtcattatc ttgatgatat tactttaatt gtttctgtta
    44581 aaactgaaaa tagaaatggt gaaacaattt atccgatgga aactcagttt cgcgaattcg
    44641 ttgatatttc gcctgacggt attccctggg gatttgagcc gcaacccttc tggtttaata
    44701 tggatgagca atacaatacg tctccagcta tgagtaataa tccaaatact tggccggatt
    44761 actggcccga tcaagatatg agctggaatg gttactggaa tggttatttt gggagaggag
    44821 tgttgaatgc tgatcttgaa accattttcg tatttgacga tgatcaggat aaagaaccta
    44881 atgtaaagtg gaatttttac tgtgatgagc gggataccac gcgcggtggt atcggactag
    44941 tcgtcaaagc tcgcggtttt caatggagtc aggttctggc tgaagattgc atattctggc
    45001 tatatgatat taagaacgaa tccacccatg attaccttaa atgctatttt gctcaatata
    45061 ttgactgggg aatcggtgga gttggtggcg atgttcaaaa catcggtgaa tatgatactg
    45121 atctcgatat agcctttgcc tacggtcctc caggagcggt gggtacacct ggaaactggt
    45181 atccgattgg ttacgctggc tatgctttct tagaaagtcc ggggatttca aatgatggta
    45241 aagacaatga taatgacgga atgatagacg agtcacgggt tagtgatgga cctggagttt
    45301 acattagtga ctatccctat ggttttactg atgcccagcg ggcaaagttt attgaggttt
    45361 ataattatga accaagagct cattggtcag ctgatgaaga ctgtgactgg acacctttta
    45421 cggatatcaa tggtaatggt gtttgggatc cggatgaacc gctaaatgat gataaaggta
    45481 aggatggtgt tggcccttat gatcctcagt attttgggcc ggatgaaggt gaaggtgacg
    45541 gcataccgac agacggtgaa ccaaattata atgcgactga tccggatgaa tctgatcaaa
    45601 tcggcttaac tggatttgaa attttcccga ctcacaccta tgagcttacc aatgatgaag
    45661 aaaattatca tgtttttgaa agatctccca aaccggtaga tcaattactt cagcctaata
    45721 atctcagcat gtttttttca tctggtccct ttcccttgat gcgaaacagt attgaacgct
    45781 attcaatggc tttattattt ggtgaagacc gcgatgatct ctttaagaat aaaaagaccg
    45841 ttcagcaaat atataattcg gattattctt ttgctaagcc acccctcaag cctaatttga
    45901 aagtctatcc gggagatggt gaagttacat tagtttggga tgatattgcc gaatcttcat
    45961 atgatccctt tttacaggag tatgacttcg aaggttacct gatttatcgc ggcactacac
    46021 ctgattttct agagagcaaa gtcattacgg atgcttatgg gaatactact tatcgcaaac
    46081 caattgctca atttgatttg caagacggta aaaagggccc gcatcaggtt gcaatttatg
    46141 gcgtccagtt taatcttggt gaagacacag gtctgcgaca tacgtatact gatagagatg
    46201 tcaagaacgg tcaaacttac tattatgccg ttgtatccta tgattatggt ttgtatcatt
    46261 atagtgaatc cggagtagaa ggattgccac cttcagagtg ttcctgtatt atcggcacta
    46321 attctttggg gcaagtgact tttttagatg ttaattgcgg gatggcgata ccccgtccag
    46381 ccagcgccgg ctacattcct ccgagtcttg atggagaaat agcgcatgtc ggacccggaa
    46441 ctggttcaat agctattgat ctggttgaaa aacataaaat accttctgga ctaacgacct
    46501 acgagatcct tttcctggaa gattccaaat accatactga aactcatccc tggtatcagg
    46561 ttcggaatgt tgtagctgat acactggttc tggattctac ttatgtcaaa tcatcaggag
    46621 ccgaaagtcc cgtactcgac gggtatactg ttaccgttta taatgatact aacataactt
    46681 tggatgaaga taagagcggt tttattgaag gcacttcgga ttatatcctc aaagtcaggt
    46741 taaatccaga taatgagaat gtagctggtt accgaatgaa tttggaattg ccggcagatt
    46801 ttctggttat ttttcatagt gacttttaca agaattcatt gaagctctgg agttattatt
    46861 cctgtccttc aaatgtccaa gtttataatc ttaccgattc ggtggaaatg gattatgcaa
    46921 ttattgacaa tgatagtagt ttaacctata ccagcggtga tgatattgct ctaattatac
    46981 ccgatgatag ttttattcta aaaaggtata cttcttggat gatccgactt agcccattgt
    47041 tcgaaattga taccagttgg gttaatggtg tcccctatgc cgataccatc tggacgacaa
    47101 ttgatccacc tgaaccgggc gaaaaattat ttataaaaat gaaaaagccg tttagagaag
    47161 gtgatatctt tcggttttcg ttgcacggtg ccgattccag cgacgtacta gccaaagctg
    47221 aactggacaa tatctgtgtt gtgccaaatc cctatgttgt caccgcctcc tgggaaccag
    47281 cgaacactta taagttcgga cgaggtgaaa gacgcctcca tttttatcat ctacccagac
    47341 aatgtacaat tcggatttat aatgtgcgcg gtcatctgat tgacacaatc gtacataaca
    47401 gtaccgcaga taacggaatg gaaccgtggg atattctttc taaagaagat aatgaaattt
    47461 cgtacgggat ctacatttat catgtagaag ctcccggcat tggaactaaa atcggaaaat
    47521 ttgcattgat taaatgatta aaacttctgg attaataaaa cattttttat tggtaagctt
    47581 attgatagat atatatctat ttaatggttg tgcgcaaaaa acagaagaga atattgttgc
    47641 caaagtagca gatagaacca taacagctga tgaatttgct tttgcctatg agatggcacc
    47701 ccgttcaata accagcctca ccagtaaaca agcccgccaa gcagttttgg atcaattaat
    47761 tgatagaatt gtcctcgccc gccaggcaga gaggttgcga ttagacgatg atacactcct
    47821 acaaaggacg gttgattttt ataaaaggct ggctgttaat cgtgaactat atttgaaaca
    47881 tattcgccga ttagttgagg tttcggaaca agaagaacgt caggcatttg aatattcgaa
    47941 aacaaagtta tacgtgcgcc atttcagtac aaagcagttt gatcttgcca ggcaagtgtc
    48001 attgggaatt gtgcaatttg aacatactcc gatatttact ggtgcccctc aggttgaatt
    48061 gaccgattat ggaatagtgg ataaaattag ctggcgcgat attccgcagg aagttgcaaa
    48121 tatactgtat aatttgccat taaggcagta ttccgaccca tttttcgacg gccagcgcta
    48181 tcatgtattt caggtagttg aattcgaaaa ggaagttcta ctgcgggaaa gtgattttca
    48241 agccaaccgt caaagtctca atggtataat tcgacaaagg aaagagcaga aattatctgc
    48301 tgggtatgtt cagaaaataa tgacgcgcca aaatgttata ataaaagctg aagcgcttaa
    48361 tcgtttgact gaggaagttt ggcgtaaccg accaactgag gtggtaaccc ttgagcaatt
    48421 catcactaat cgcgaaataa atactatagg cgaatccagt cgggattttc ttaataaacc
    48481 gattgcaaca tttaaatctg gggagatgac tgtagccgat attttgatca actataaagt
    48541 caatccggtg aaaatttctt ataaaagtaa aatgactgtc cgtgaaagct taaaaaatgc
    48601 agtgggaatg tatgttcgcg attgggttct atctgaacaa gggatgaagg agaggttaga
    48661 tcgtaagccc tcagtaattg cagaagaacg gagccggagt gaatatcttc tgatgcgtaa
    48721 aatgctggca tatctaggtc gtgagtttta tgcccagaat caggacacaa caggcagaca
    48781 ggaacaactc gaaaaataca ttgccaatta tgaagacaat ttacgaagag ctgaaaatat
    48841 cgaaatttat agtagcaact taatggctgt taaaacaact gatgaaggac tggcgcgcaa
    48901 aattgacttt gttgtttttc atacccaata aatgaggatg ttatgaataa atcaatgtac
    48961 aaaaaggtat tgatattcat tttattaata aactctctta gtatgggcag ctcaaaacga
    49021 ttgactaatg tgggtacgac tgccgcacct tttttagaag tcggagttgg atcgcgtgcc
    49081 attggaatgg gcggggcttt cgttggtgtc gctaatgatg tttcggcgct atattggaat
    49141 ccagccggaa tcagtcgtat aacaagtcca gaagcggtgt ttgagaaagt cgaatggttg
    49201 gctgatatca gtttcaatta tattggagct gttattccat ttggtcgctg gggaacagcg
    49261 ggattttttt tagatgctat gacggttccc aaaatggctg tgcgtaccgt agattatccg
    49321 gaaggaaccg gtgaaatgtt tgacgccagc agtattgctt ttggcttatc atattcctat
    49381 gcgttgactg atcgcttttc cattggacta aatggaaaat atatcgagga gcgcatctgg
    49441 catgaaagat ctcatggtgt agcctttgat atcggaacac tttataagac gagttttagt
    49501 ggtttacgaa ttggtgctac gattactaat tttggtactt ctatgtcttt agatggcagt
    49561 gaccttatta tttattatga tgccgatgaa agtattgatg gcaataatga tcgcattatg
    49621 ggaaaattaa tgactgatga atggccgcta ccgctaaata tgcagtttgg tctcgcttat
    49681 gatttgttga gtacaaaata tatcaaaatg acgattgccg cggatgcttt ccatcctata
    49741 aataacagcg aaagtatcaa taccggaact gaactagcaa tttatgatat gctattcatt
    49801 agagcaggtt accaggcaat cggtcaaact aataccgagg aagggctaac atttggtgta
    49861 ggattgcgtt ataaattatt tggccagtcc tatatcaaat tcgattatgc ctttgcagat
    49921 tttgggcgtc taaaaaatgt tagccgatac acattacggt tggattttta aagtaatgca
    49981 aaaaatgtat cctgattttt accaggataa tactcagtgt catttaaaag tgagtacagt
    50041 acaatgtcta gaaaaccatt aattatttta tttcttatac taaatatgtt tacttctatc
    50101 aaagctcaac agattaatat taaccgggtc gagcaaatgc ctaatttgcc aacaccatat
    50161 tttatgcgtg actggaaaga agtttctcgt ggttatgata ggttagtctt cgatctaaac
    50221 cgtaccggac agtacttacc gttggtctgg ctcaataata ataccgttaa ttatcccaat
    50281 cattccagtt ttggtttgca tacggttgtt ggaacaacct atccatcgaa tgcggaagca
    50341 attaatgttc tcccagcagt tataggagca acactagttg gaattgacaa gagcgaccaa
    50401 aatggatata attgggtact gggctgtgaa gagtggttta accggcggcc agaagaaaat
    50461 gtctatttaa acagtccgac ggctcaaagt ggcgatgatt ggtggtatgc cgtgatgcct
    50521 aatgtttttt tttatcagtt atatgacctt tatcccggta ccggcgattt tgattatcaa
    50581 tttacttcag tagcagatca atggctgaga gcagtcgttg ctatgggcgg cagtgcggcg
    50641 ccctggcaaa aaccgaatat gaattatcgt ggctggtatc tctcgacaat gaagccgttc
    50701 acctctggtg tacccgaacc ggaagccgct ggtgcgatag cctggctact gtacaatgct
    50761 tacactaaga ccggcgaagc caagtaccgc atcggggctg agtggtgcct cgagtttctc
    50821 aatgcttttt ctgctaatcc ttcttatgag ctgcaattgc catacggtgt ctatgtcgcc
    50881 gcccggatga atgccgaaca gggcacaaat tataacatcg agaaattgtt gaactggtgt
    50941 tttgatcctg agaataatct ccgcaattgg ggcgtgacgc tcggtaaatg gggtgattac
    51001 gactgctatg ggttagtcgg tgaagcgaag aatgaaggct atgcctttgc tatgaacggt
    51061 tttgaaatgg cgggtgcgct ggtgccgatg gtgcgttacg acagtcgctt cgctcgggca
    51121 atcggcaagt gggtcttaaa tctagccaat gcctcccgtc tattctactc acctttcttg
    51181 ccggacagcc ttcaggatgg cgagacctgg gcgaaggaat acgatccaga aggctatata
    51241 gcgcacgaat ctatgcgtga gattcatcca aactggggat acagtccctt tgcgaccggt
    51301 gatgccatct ccggcggctg gggagccacc aatttcgcat tgtacggttc atcgcatgtc
    51361 ggtattcttg gtggtataat tgatacgacc aatgtcacag gaattttaaa actcgatctc
    51421 cgcaggaccg attactacca gaaagatgcc tatccgagtt atctttttta taatccttac
    51481 agcgaggtta aatcgatttc gttcaatgtc ggaacacagg cagttgatct atacgatgca
    51541 gttagtaaaa cttttattgt aaccggaatt accggtgaaa caaatcttaa cattcccgcc
    51601 gatggcgcgg ttttggttgt cctggtacct tccggcggaa cagtccgcta tgtcgaggaa
    51661 aaaatgctgg tgaatgatat tgttgttgat tatcattccg ggcaaactgt gactaattat
    51721 aagccgcgta ttaaagccat tgctagcgat tcaactgcgc tcaaagccgg gagaatctat
    51781 aagatttatt gcaccgcagt agatcgtgac gacgacgaat tgcactattc atggcaaata
    51841 aatgattcag aaatcggctg tgattcttct atcatagaat tgacaatgcc agagcttaat
    51901 actactttaa caattatttg cagagtcagc gatgaggtga atacaccggt agcggatagc
    51961 atccaaatat tagtttatac ggttcaaaac caggtaccaa tcatttccag tctgaccgct
    52021 tccgcgcgtc ggattgcgcc gactgataca accttgatta attgcagtgc cagcgatccc
    52081 gatggcgatt cgctgatatt caattggtca gccgcaagcg ggatactgga gagtgacagt
    52141 gcgcaggcag tctggcaagc gccggactcg tccggttatt ttttaattaa atgtactgta
    52201 agtgacctgt ttggcggggt agctttagat agtattctta tcgttgtcgg tgatacatcc
    52261 ggtcatgaaa ttggcgctcc gctggcgtac tttgcttttg caggcaatgc cgacgatctg
    52321 agcggtttta ataaccacgg tactgtcaac ggcgctagtt tgagtgccga ccgctttggt
    52381 aaattgaaca gcgcctatta cttcaatggg acgtccgaca atatccgggt accgaatagc
    52441 gttagtctga acttcactaa agcgattaca gttagcttct ggataaatgc cgctgaactc
    52501 tataccagcc gcgaatctta cccaatctct cacggcaatt gggaaaatcg ctggaaagtg
    52561 tccattatcc cgcaaaaaaa aatccgctgg acgattaaaa cgacgactgg tgttaaagat
    52621 ctcgattccg ataaaacgat taatgtcaat caatggtatc atattgtcgg actatataac
    52681 ggaatagatt ttgatttata tgttgacggc gagttaagca accatactac cttttccggt
    52741 gatattttaa ccaccaatat cgatttaatg attgggcagt gtctgcctgc agtaagtcaa
    52801 tacaatttca agggtgtgat tgatgatata cggatttatg actacgcttt atcaataggt
    52861 gaaatcagta aactatatac tgagccgagc gctattcgtt ccgataccaa ggaaatgccg
    52921 acttcttata aactatctca gaactatccc aatcctttca atccggtcac cacaatcaaa
    52981 tacgatctgc ctcagaacgt tccggttaaa ttgcaggttt tcgatttgnn nnnnnnnnnn
    53041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    53101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa tcaaatacga tctgcctcag aacgttccgg
    53161 ttaaattgca ggttttcgat ttgggcggaa ggctagtaca aactctggtt gatgaagaga
    53221 aaccagccgg acattacaca attgtctggg atacattgaa tatcagctcc ggcatctatt
    53281 tttaccggtt acaagcgggt gagtttacct cagtgcagaa gtgtatcaag ttaaaataag
    53341 gatccaaatc taattatgtc taaaacacat ttgaaatcaa tcttctattt aaccttaatc
    53401 ttgaccttga cctcattttt cagttgttcc cccaagagta gcattggtac cgataagtta
    53461 gccgtgcaat tcaagggcga tgccaagatt gaagtcggtg ggccttttgt cggcgccgaa
    53521 ttccatcata gctatatgat tcctcagcgg attagttttt tctatccagt agctaatagc
    53581 attgatctga gccgcgatta ctggacccgt gatagctcct ttgttgccga ctggacatta
    53641 aaaatcggca attctgcaga tatcgaaatc gggaagaagc cggcggaatt cgaccttaca
    53701 ccgtattctg ttgattttca ttataaagat gatgaatata tagtaaaagc tgcatatcgc
    53761 ttttgtaacg aaaaaccggc aatggttgtc gagatcgaaa ttgtcaataa ttccggtatc
    53821 gctcaggatt ttcaattcga taccagactc agaacttcga tccgcacctg tcataccttt
    53881 agagaaattg acaaggcggt ttcagaaatt gaagacaata cgatttataa taatttttat
    53941 gatgccgatg ctgattccgc cgttgtgttt atcactaatg atggcgattt gccggtagag
    54001 tgtttgactg ctaatgtagt tcaattacct ttcgctggat ttatttataa aaaggggtta
    54061 aaacctggcg aatctctgaa tatcattcag attatcggtt cgtcaaatcc ggaagaaggt
    54121 aaaaatttag tcaaatatct tcgggcaaat tatcaattgg aaattgacca gtatgaaaaa
    54181 tacgtaaatg aacaggcttt cctgagtagt ctaatgaaaa ccggttcacc ggcaaccgat
    54241 cattcggtgg cttacgctaa ggcggtgatg tccgcgaacg cccattacct agacggtgag
    54301 atcgtcccaa tgccctgtcc cgcggagtat aatttttatt ttactcacga tgctttggtt
    54361 accgacctgg cggcggtgaa ttttgattta gcacgcgtac ggcgtgactt ggattacatt
    54421 gttcgtcatt ctgatgaaaa caatgtaata ccgcatgcct attactggaa agatggtaaa
    54481 tatgtgactg agtttgccag ttccgataac tggaataatt tctggtttat tcaggtagcg
    54541 gctaaatatc tgcgccactc tggagattcc gactttctta cacagcttta tccttatttg
    54601 acggttagtg ttgagcgctc cttattgact ctcgagaagg atgatctgat gtggtcgtac
    54661 cgtccggatt ggtgggatat cggacataat tatggcccgc gtagttatat gacgatttta
    54721 gcgattaaaa gtttgcgtga ttatgcttat atctcaacag tgctggggaa aaatcccgaa
    54781 aaagtagttg aattgatggc attggccgat aaaatgcaaa cagctctagt ggcaaagcta
    54841 tggaatgatg atatgggcta tttgataaat tataacgaag atggatcgct cgatagccat
    54901 tattatattg ggtcgctatt ggcggtgcat tacggattac ttgacaaaga aaaacaaaat
    54961 cgactagtcc agacagcgat ggccaaattg cttgatgaga aggttggtat ttataacgtt
    55021 tatccgatgg attttcacct gctgggagat ttttataagt tcgttggcaa tgaagtcggt
    55081 gataaatact attatgccaa tggtggggtc tggccgcaag gcaatgcctg gtacgctcta
    55141 gcattaattg ccaatgatga gaaggaaaaa gcggctgggt ttattgagaa tacaatgtcg
    55201 ctctatggga ttatgaatgg tcccaacggt caaccggctt actatgaagt tagaaatgct
    55261 aataaagagg atcctacaga atacggaacg gttgataaac cctgcttcct ttgggctggc
    55321 gcctggtata ttagcagttt atatcggctc tatggtatta ctgaaaatga ttggaacatt
    55381 gccttagatc cgtatttgcc aaatgatcaa aataattgcc aattcacgct attttttaat
    55441 ggtcaaccgt tagtcattga aataaaaggt cgtggtaaaa taattcactc ccttaaattt
    55501 gatagccagt tagctcattc agcagtattt cccattgata tgccggtagc aaaaaaaatc
    55561 gaggtcattc ttagtgattc accgaaagag ccatatttaa aaagcactaa cgctattctg
    55621 cttaaatatg agtatcagaa tcggttatta tcgctaatgc tccgtggaat tgctcctcta
    55681 gaaaatgaga caattgtcat tagtccccaa aaacctgtaa gtatctcact aaacaaccgg
    55741 atgttaaccg aaggatggtc aactgacaaa gccgacggct ggtatgtgac gaaaatccgg
    55801 tttagaatga ctgcttccga tgataaatta tgcgtgaagt tttagaatga aaaaatgatt
    55861 tataacaaga cgacagttgt tattttcctc ctttttttgg tgattgaggt ttcctgtgaa
    55921 aggaaacagg ccgcccaatt taaagatgag caggatatta ttcgattgac ttactggtgc
    55981 gcatccaacc aagatgaaat caatctagca aaggaattgg tcaacgaatg gaatgctgaa
    56041 catcctaaag ttcaggtcgt attacaaccg attccggcta gccaatcgtc ggaagaagct
    56101 cttctggcgg ctatagctgg taagacaact ccggacgtat gttccaatat gtggccggga
    56161 gcgatggatg attttacttc ttcgggcgga ctggtgcggc ttgaccagtt tccggatttt
    56221 atagaatata tgcaaaagcg tatgccagag gaattactcg aatcgtttag atctcctgat
    56281 agtcattatt accagatacc atggaagact aatccaatta tggttatgta taataaaaaa
    56341 atgttcaggg aagctggtat tgtcaggcca ccgcgaactt atagtgaata tttggcggcg
    56401 gcagaaaaaa taacgaaaga tataaatggt gatggacgca ttgatcgctg gatggggtat
    56461 cgtgatattc ggccaatttg gtggcaaaga ttattcgatt attatccttt atatatagcc
    56521 gcatcgggtg gtatgacact atttagtggc agtgaaatag cctttgccaa tgaagcctcg
    56581 gtaaaagttt tccagttttt caatgagtta tatcgttatg gttattatcc tctaacaact
    56641 tttcagggcg atgctttttt agctgagcag gtggcaactc agtttactgg tccgtggaat
    56701 gttattcacg tggagaattt taaacccccc ggatttgaat atggcatttc tccgattccg
    56761 gtacctgatg attataatgg tccggtttat acttatggtg atcataagaa tatttcaatt
    56821 tttagtacaa ctgaacatcc ggaagccgcc tggcaattta ccaaattcct gatttctgcc
    56881 caggctgatt taagattatt ggagatttgt agtcagattc cattgagaaa aaatttgacg
    56941 gtagttccgc tttatcatga atattttcta gagaatccaa tgatgatgca gtttgccgag
    57001 caagcagttt atacccgagg cgtggacggc tcatcagtat tgaaagaaat ttttgatgct
    57061 atttcgcagg aatatgaaat ttgtgcggtc tataacaaac gaatgcctca agaagcagtt
    57121 gctaatgcgg ctaaaagggc tcaaataata atcgaatgga atcgtaaata atgaaagctg
    57181 gttttccagt aaacaaaatc aaaaacaaaa ccggctacct gatgagtctg ccttatattt
    57241 tgtatttttg cctatttatc ggctttcctc taatcttttc ttttattctg attttccata
    57301 aatggaatat tgttacaccg atgcaatggg tgggtttgcg caatttctac cgactttttc
    57361 atgatgttca attctttacc tcgattcgca atacgcttat ttttctggta atccatattc
    57421 cattacaaat tgtaattgca ttgtttttgg cagttatatt aaatcaaaaa attaaattta
    57481 aaggcttttt tcgagcactt tatt
//
LOCUS       DAOOMU010000004        55672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_391_length_55672_cov_12.567866, whole genome shotgun sequence.
ACCESSION   DAOOMU010000004 DAOOMU010000000
VERSION     DAOOMU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 55672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55672
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_391_length_55672_cov_12.567866"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1243)
                     /gene="ugpC"
                     /locus_tag="PLI55_00720"
     CDS             complement(161..1243)
                     /gene="ugpC"
                     /locus_tag="PLI55_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HPA99393.1"
                     /translation="MASIELKQIVKEFDKGAPIIKNLDLTINDGEFLVIVGPSGCGKS
                     TTLRLIAGLEEVTRGEVYIDNRLVNNILPKDRDIAMVFQNYALYPHMTVYENMAFGLK
                     LRKYPKSEIRQRVKEAADILELGDLLNRKPKQLSGGQRQRVALGRAIVRKPKVFLFDE
                     PLSNLDAKLRVQMRTEIKKLHHHLKTTMVYVTHDQVEALTMGDRIVVMKDGEIHQIGS
                     PSEIYQNPQDLFVAGFIGSPAMNFIPGVLHQGVFQSNTNNIKLILEKSPYKEMDNVAV
                     VLGIRPEDVHLNKQDAHYQEVRLTLEVVEPLGNESILYFSDEGHQITLRTQETNMPLS
                     IGASIPVFFNIDKVHIFDQITEKAIR"
     gene            complement(1351..1881)
                     /locus_tag="PLI55_00725"
     CDS             complement(1351..1881)
                     /locus_tag="PLI55_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPA99394.1"
                     /translation="MSNILILYDSKTGNTAKMAELVALGARANGDHDVKILRIDDAKA
                     EDIRWCDGIALGSPTNMGIVSWKMKKFWDDLGDELWGKIEGKIGCAFSSSGGWGGGAE
                     ITCQSALMILINFGFLVFGVTDYVDEQLTLHYGVALAGEPRSAAEKAACLRLGERLAE
                     YLSKFKLQSDKNINEV"
     gene            complement(1945..3264)
                     /locus_tag="PLI55_00730"
     CDS             complement(1945..3264)
                     /locus_tag="PLI55_00730"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA99395.1"
                     /translation="MKNRKIKRLSIVGFVFLIVMANNCSKPKPRDANSITLTFWHSFV
                     ATTLPILEAMIADFEEENPGVKIQAQYVPTGDALVQKLITAIQSQTAPDIAWVHSDFL
                     DNLVNSRAIFSINELAGDDSLFYNQCLPDIFPQLLPPITFRDTLFALPMEATTLALLY
                     NRDWFRQIGLNPDHPPATWAELDSFTMQLCSDSNSDGQPERYGFYVPVFPASGPLNIW
                     MTMQWAPFVWQAGGEIFSNNQPTFASEAGVAALAFWSKIYHQQKFERFSMAHDMGFIS
                     QTVAMIMDGPWNLPRYRQYVKFDWGVAPLPAGSAGRASYLAGESLVVFKQCVNPEAAW
                     RFINWFLKPEVQRTFAEKSGYLPVRRSVLNDSDYVNFLEQFPQQKSFNEQIRYARGRS
                     LPDKFRVEVNQCIAEALESSIIGRIAPEKSLKVADEKIRRLLSSETE"
     gene            complement(3254..3847)
                     /locus_tag="PLI55_00735"
     CDS             complement(3254..3847)
                     /locus_tag="PLI55_00735"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPA99396.1"
                     /translation="MPVISYKNEPFPDWSEVKSCKIIGLSKRQKLQLPSEFSETLLFV
                     LDGVAEISEGAEKRTIFSGDVLDQFFGEVWIEPKPLVELFQISGNWSNDRGSCGVFRL
                     GKSEHPRNDGDPVEYERNTVFDNHFHDCDEYWFIIKGKGMVVSEGESYAVQAGDCVIT
                     PAGEHHDFPIVQEPILAVWFEGSMKGKKRPGHLYREK"
     gene            complement(3828..5051)
                     /locus_tag="PLI55_00740"
     CDS             complement(3828..5051)
                     /locus_tag="PLI55_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99397.1"
                     /translation="MNEIISKLEKLIEHNDGILRLKPAWVARDFLPPGRRLGLPEEMY
                     DLGERGGICERWVGSTTPADNRIKVPNEGLCCLDLEDGQEITLKAAVEIAPALIMGAD
                     YAKAHTGLGRLPKIFDYAYRLPYHLHQMAKDARKVGRNSKEEAYYFPEEVPLGPEPET
                     YFGVHPYISLEKRYELLLPSMQEWKDDSILLHSRAYRLKAGDGFHVPAGILHAPGSVL
                     TIELQEDSDVFAMMQAKVGGDIIIDKELLFKDVSEKERKKLAEKAILGMIDWELNGDP
                     YFYENRHTPPILIDSDQQSGSEQSWIFYNTHKFSGKKLIIRPGGKHVSVEKGVYNILA
                     WRGQGTYGNVEIEGLNFEKDELLICHQRAIYPIEVVNTGDRDLIIYKFFGPDINPDVP
                     MLTKYPKSPNACNIL"
     gene            complement(5073..>6890)
                     /locus_tag="PLI55_00745"
     CDS             complement(5073..>6890)
                     /locus_tag="PLI55_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPA99398.1"
                     /translation="VRDGKFYLNGKPFYMIAALDQDFYPETVYETPSEEYLYDEMQKA
                     RELGLNTLRCHIKVPDPRYLKVADELGLLVWYEIPNWDVFDESVKARSRETLQEMLQR
                     DWNHPSLAIISLINESWGLDLNQANQRQWLKAEFDFAKSLANGRLIVDNSACWGNYHL
                     KTDINDYHTYWAIPENHQKFRETVQEVSDRPRWLFSPYGDAEETGQEVLMISEFGNWG
                     LPKLPKKLPFWFDRQFLDNLVTLPAGVTQRFGDYQYGRMFKNYNELAEFSQKAQAQAL
                     KWEIEEIRLQPELQGYVITEFTDINWECNGLLDMWRNFKADRQILAQVQQPDIIIPRL
                     TKYGFWDNEEIDVQLSISHYSNRDFSNVRIQGIYNGEIIGDKSIAIDSVGVYFVGSMK
                     INSEPVKQPVSQRVKFRLLSREGDVLAENFIEFTVYPSRKIENISPNSNLLICNSIDL
                     KTMDHLKNGLDVICVIDSSSVLPADFPFTITSRESDGYDGNWASNLNWVDNSHRPFNN
                     LKSNKVLGFEAYKALPKYAITGIQPEQFVDVLAGMYVGWLHLNSAYLMQMRVGSGKLI
                     LTTFNLIQNYGDDPFTTTLRDELVAYLQSEDCQPTINWK"
     assembly_gap    6891..6990
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6991..7479)
                     /locus_tag="PLI55_00750"
     CDS             complement(<6991..7479)
                     /locus_tag="PLI55_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPA99399.1"
                     /translation="MITSCDKNPVVDLNGRWQLAPDSTGEITINSIDLIENWRMTEIP
                     GSWHRLHVDLLDYQGVVWYRKSFKLKSLPPKHRFLLNFGAVDYLAKVYVNRELVGGHE
                     GGYTPFSFDITERLKQGENEILVRVVDPVADEVGTDGISYWHIPHGKQNWYVQTSGIW
                     QAV"
     gene            complement(7633..>8264)
                     /locus_tag="PLI55_00755"
     CDS             complement(7633..>8264)
                     /locus_tag="PLI55_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPA99400.1"
                     /translation="LIQIRQRYSALRSDYFKEIPVVPAYYAFAFHRRSGDENLFSVIN
                     MMSEPKNITISLPIEELHLDSTKTYYLSDLITGMTFSGKPENLSSVALTIPGYTTSLF
                     LLADSMVNVNIAPEEHPPIADKFTLFQNYPNPFNSSTTIGYTIPQKSNVKLTVLDLLG
                     REVMELVDSEQNPGEHQVLIRADQLASGCYFYRLKYKDQILVGKMLLLK"
     assembly_gap    8265..8364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8365..10139)
                     /locus_tag="PLI55_00760"
     CDS             complement(<8365..10139)
                     /locus_tag="PLI55_00760"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPA99401.1"
                     /translation="MRNIVKIITLIGLGLASTLAVELQWQTHNIEVWQPEQVIWGKID
                     TLTATSGVLYWKDGSEIFDINPSDSSFYIEVKLDEGENWFVATIENQGEPIYSDTLRL
                     QMKMSLKPILEAYATVTDREVTLHGKIIENPDSEAVTFFWSEDGRNPASFGLNEITDS
                     LLVFNVPEEVPNGEYYFNLAGVTASGDTARARTFITVSPDDIQPFDIRRDHAAWIDSA
                     VIYEITPYAFVMEGTFQDIQQKLSELRELGINTIWLQPIFKTTWGEQGYDVIDYFNIR
                     SDYGSESDLQNLIKSAHTLEMKVLLDIVINHTSIYHPYAQHSIEYGSTSHYYDYYQRK
                     IDDAPYSQYYNYYKGFINYFWDELPNLNYDNPEVRNWMMTVCKYWVEKFDVDGYRFDA
                     VWGVNARCPEFTQNLRLELKRIKPEVMLLAEDKASWSMVFDQRFDVAFDWAPTEDWVS
                     QWYWEVDYSEDATKQYTIFNYGNPSERVNLLRQALTNYGQGYAANAKILRYMGNNDMI
                     PFIKNHNLAQTKMVSTLIFALNGMPMLYNGQEIGYNQHPYSSYQIFQRGKSIRSQDKY
                     GLFDHYRHLIQIRQRYSALRSDYFK"
     gene            complement(10172..11320)
                     /locus_tag="PLI55_00765"
     CDS             complement(10172..11320)
                     /locus_tag="PLI55_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPA99402.1"
                     /translation="MTSKERLLIALKGGVADHVPVSPDISVMVPDRLDGRPFYEIHLD
                     GREHYGWTSATHGEVYVKAVKYFDMDGFYMYGGLKEVKVSSSLDFESETVDIPAGKLV
                     RRTWHTAKGDVTEEEIFFADESPWRSVKPIKNLQEDFEKFQLVMGKGDWQWEKEFRDK
                     QVIGDLGVYLGMIPVFQDWWFSNRDGGFENVFSDLLMEADYMQKVHEFWMNWALAYLR
                     AMIEAKPDVIMLGGSSASLSVSSPDIFRKYELPFIQQASSICKAANMPCHLHICGRSW
                     PLVQIVAEETDVTSMEPMEEPPTGNVDIAMVKKRYGRKLNIKGNINTTEFMMRATPQQ
                     VEDKCKRLIDDVGYDGGFVLSTGDQCGRDTPDANIFKMVEVAKTYGKY"
     gene            complement(11331..13007)
                     /locus_tag="PLI55_00770"
     CDS             complement(11331..13007)
                     /locus_tag="PLI55_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPA99403.1"
                     /translation="MNNDPSLKKTLSRREFIRKSTMGIVGAAMFPSFLEKSNILAKTT
                     KPLLFKPFPHPWMPPLNFVYAADTNGDPFQSGIVVNGDGIVVPEDLGTRRFSVNCQWF
                     IEGFGNCFLSADNDGEFYSLKEMTGRNELILNHEFARSRIARNRQVMRRYQTQGTKFS
                     PEVIRLSGLAEEYLEDSAKYTAESEKCASIADKALYYALWTGENIELEHARQMISKNN
                     RQDEVYFGCESRQYVWAKNEEFTKRFVELFNFATVTHYIWDTWYELFEPREGVYNWGI
                     KDNIVRWLTEKNITIEGRPLFWFHPTVTPEWLKSKNYDQLRSYVDKHISDVVGHYGDQ
                     VLHWEVINEYHDWANIFNHTPDQITEIVRQVCDKTHEINPKVDRLINNCAPWGEYVAR
                     GRMARMEATRPLRTPHQFITDLLDAGVEFDTLGIQIYFGNRDLSDIMRMLERFEKFGK
                     PIYITEIGTTSGPTFETVVDRSMGISGRPYDWHRAWDEELQADWLEQVYTLYYSKLNI
                     KAINWYDFSNFRPFIVNGGLVTESCEPKQSFYRLKELLRSWNRLPDNIKK"
     gene            complement(13008..14555)
                     /locus_tag="PLI55_00775"
     CDS             complement(13008..14555)
                     /locus_tag="PLI55_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPA99404.1"
                     /translation="MPNKTLIFKPYFIQKGRGPHLDEFAYLMDMNGDTFHSDVAVKNQ
                     GVTISNTKGIKKFTLNVRWNVEGYGYLFMPADNGGEFYSLPQFGSLQLNLNFELAKTR
                     IYRNRRRQANFNREGWLASRELSAMINLAENYLDDAIKNVQDDETCARFSQQALLYAL
                     WASEMLELEKARWNIAKNSARLDFLFGCDGRGYFQMDSELFLERFTEVFNYATITHYL
                     IGDFVDFEPVEGQKQFAERDRLLADLEKYKIQVAGRPLFWTHKWVTPDWLKNKTFPQL
                     LNYLEKHIRTVVGHYGNRIKVWEVVNELHDWANELFLNHEQTIELTKFACDIVRDVNP
                     NLKILINNCSPFGEYVQKGLWHELPARYPQRTPFQFTEQIIEAGTDFDAIGVQVYFTS
                     KTLADYIGVIERYEKLGKAIHLAEVGAPSRGITQEFLDEEPGDYSTLPYEWHRHWDEE
                     LQADWLEAIFTYAYSQPMIQAANWYDLIDGFSFLKNGGLLRSPKGERKAAVDRLKKLQ
                     EKWRILQ"
     gene            complement(14579..16249)
                     /locus_tag="PLI55_00780"
     CDS             complement(14579..16249)
                     /locus_tag="PLI55_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPA99405.1"
                     /translation="MPRITRRKFLKMATYAGLGGIVGGRALSAPLVLTPKRTKTNTEA
                     GELIFKPIIVQKGRGPHLLDWAYASDEHWDAFHSNITASNEGVKISDTEGTQKFGVDV
                     RWNVEGFGYIYITADNGGEFYELPPKGKQRELNLNFELAKSRVLRNRRRLELLRRDGW
                     QPSREVKELLALSDSLYEDAVKSQASAELCGDYAQRALYYAMLVGEKMELEKADFEIR
                     KIGYRPHFFIGCDSRAFYQMDPELFMERFTELFNYATITHYLLSGYYQDFEPIEGQKN
                     FNLRDAVFKELRQNGITVEGRPLFWFYNSTTPDWLRHKSYDQLLKYVESHTKEVVSHY
                     GEGMYAWEVVNEAHDWANELRLKPEEIVNVTKLACEVAKATNPKVHRLINNCCPFAEY
                     VQLKKWGELDAIYPQRTPHQFMQDLVDAGVDFTITGQQMYFPYRDLQDTIILIERLEK
                     FGRPVQLTEIGASSGPTKDSINTGQLGMPTEPYVWHRHWDEELQADWLEGVYTLAYSK
                     PWIEAANWYDFVDPYAWIKTGGLLRSPNGERKAAWQRLLKLKEQWKNL"
     gene            complement(16261..17856)
                     /locus_tag="PLI55_00785"
     CDS             complement(16261..17856)
                     /locus_tag="PLI55_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPA99406.1"
                     /translation="MANITNSQSQAPSPSGELLFKPFFVQKGVGPHLGDLAFATDENG
                     DTFPSNMTIDADGIKISNTKGHKRFGINVRWNVEGFGYNDYTADNCGEFYELPIFGKQ
                     TVYNLNYEIAASRVARNRRRHNNFVKEGWQPSKETSAYLDLSEGLLEDAGRKPRLSED
                     CAQLAQKALHYALWGSEMMELEKAKWNIKRIGRRANFLFGCDARSYYEMPKDRFVEYF
                     TRLFNFAAVTYVVNGDPEMDIFEPVEGQLNFTHRDILCNVLRQNGIKVEGRLLFWFHK
                     WVTPEWLAHKNFDQLKKYVENHTREVLGHYGDRMAVWEVVNEFHDWANEVQLNPDQVV
                     ELTKFACEVARDTVPNIPLMINNCCPFAEYVHLGNWSGQKALYPQRTPWQFVRDLIAA
                     DVDFDIIGLQYYYQYRDLQDYIVNMERYESFKKRVQISEIGATSGPSEESVKNGSLGF
                     PTEPYSWRRPWDEELQADWAEALYLIGYSKSWIDSVLWFDFIDNGAYYDNGGLLSGRG
                     RHPKVAYERLIKLRQWLETSGTK"
     gene            complement(17906..>18781)
                     /locus_tag="PLI55_00790"
     CDS             complement(17906..>18781)
                     /locus_tag="PLI55_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HPA99407.1"
                     /translation="NFYYYCFSTGDRIWISCSKNLHEIGLAQPTLVWQPPKGNPWSEE
                     LWAPELHFFDNKWYIYVAADDGLNENHRMYVLKSDSVDPLSHYVLVGKIADSTDCWAI
                     DGTVLEHRGHLYFIWSGWEGKVNVQQNLYIAPMSNPWTISGDRVLISEPEYEWEKVGR
                     PLVNEGPEILKKNDKIHIIYSASGSWTDDYCLGQLTFTGGDILSRASWIKKPEPVFSK
                     TETVFGPGHASFVKSSDGREDWIVYHAAKYKGSGWDRDVRMQKFGWNNDDSPNFGAPV
                     SAGVLLEVPSGSYKK"
     assembly_gap    18782..18881
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19102..>20313)
                     /locus_tag="PLI55_00795"
     CDS             complement(19102..>20313)
                     /locus_tag="PLI55_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HPA99408.1"
                     /translation="GVGKYTDRERYSVFSLWDTYRTLHPLLTLIYPEQQSAIIRTMID
                     MYSESGFLPKWELAGNETYMMVGDPAPIVIADSYLKGIQDFDPDLALEAMLKPTLLDS
                     GESAPPIRAGYHELLKYGYIPFEQDTSQAWWVWGPVSTTLEYCLSDFCIGNMANMLGQ
                     NQIAEEFGKRASFYRNLFDPKTRFMRPKLRNGQWLEPFDPLATEGSGYWEGSGGPGYV
                     EGNAWNYTWFVPHDVSGLIDQFGGYEPFRIKLERCFSEGHFTITNEPDIAYPYLFSYI
                     PGYEYRTAELIQSILTEEFVSEVDGLPGNDDAGAISAWLVFSALGFYPACPAVDEYRL
                     GIPLFQKVTIELDKEYYSGKQIIIERSKVSANPARLEEILIDGKKSDSFGVTHKRLTE
                     GVHLKFVDSQE"
     assembly_gap    20314..20413
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20414..21102)
                     /locus_tag="PLI55_00800"
     CDS             complement(<20414..21102)
                     /locus_tag="PLI55_00800"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 92 protein"
                     /protein_id="HPA99409.1"
                     /translation="MRFKISLFLLFFLFINGCSSSGINDLNQFVNPFIGTSGGGNTFP
                     GALVPWGMVSVSPHTDLKAPSGYIHGEPYFYGFGQVHLSGTGCADLGSVIITVTRGEV
                     KTEPEQYRCSYRNEQAKPGYYAVNLNEPDVKAEVTATTHCSFARFTIGKDGNVNILID
                     AGRSLVERQGGSVRFVSPTEIEGYNRSGGLCGEDNQHQVYFVTRVSATPQKFGTWIDD
                     QVSEDISRASD"
     gene            complement(21120..22262)
                     /locus_tag="PLI55_00805"
     CDS             complement(21120..22262)
                     /locus_tag="PLI55_00805"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HPA99410.1"
                     /translation="MEHSKRIYSLDVFRGATIAAMILVNNAGSYDYVYPQLLHAEWHG
                     WTFTDWIFPFFLFMVGMSMVFSFESRLKHGDTHQQILRHSLKRLLILFALGLFINGFP
                     FGLIPGSQFSIATWRILGVLQRIGICYFFTSLIYLNVKPKGQWLWIGILLGIYWFALK
                     VIPVPGYGAGVLEPEGNLAWFIDKTLLGKHTWAFAPVFGFDPEGILSTIPAIASTLLG
                     VQAGRWLRSERSQEEKTAGMFVAGNLCLLLGIIMNIWLPINKNLWTSSYVVFINGWAL
                     NCFATIYWIVDVKGKTWGTKPFVIFGMNAITVYTLSELLANLLWAISWNRADGTFTTL
                     HDAIYENVFLPIASPVNASLLFAIAFVLMMYAVAWGMWKKRLFIKI"
     gene            complement(22271..>22777)
                     /locus_tag="PLI55_00810"
     CDS             complement(22271..>22777)
                     /locus_tag="PLI55_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99411.1"
                     /translation="ILPWKDRDFGMNLAPHGWFAAEYRILLRNMMVREQSDTLHLLSA
                     ISPNWVKKDAKIDIKKAPTEFGEVNFTLTFKKNSATLVLKNNFKKAPAVIILHLPWFM
                     EVRKIEIDGRSINQENNAVSLPVDTRQVDLEWSFKKNIENMSYDQTVEQYKSEYRRRY
                     EKFIREGD"
     assembly_gap    22778..22877
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22878..24427)
                     /locus_tag="PLI55_00815"
     CDS             complement(<22878..24427)
                     /locus_tag="PLI55_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99412.1"
                     /translation="MKNWLYLWLIIILFGNELNAQMLNPSIDRNDEPFCYFSKPTDQI
                     GVMDGREGTLITPEGYLYTGSGEMMFFTGNPPIPTEKRVKTLLNGYLPVIQYALNRDG
                     FDYRWTIFAATLDGKPESPLVNFVRVKIINRNSQQRTTWFAAGIRYQNEANTDWGIGD
                     NRFIRPAVPEQIGEYEQAGLEFRQDWLCLHQDDAVLYNDKLLYSFPPREKIRKFLTWK
                     MGYNEPPAEGEMTLYVLPTTPVGIVQYPLTMLPNEEVVLDFKFPYMPVDTVDEYSKWI
                     RQAQFDHYLNKTIQFWENVLQEGMTIVLPEKKVTDTFNANLINSLIARDKIDGWYVQK
                     VNEFQYDNFWLRDAAYFTRMYDLTGYNNEANRILDFFPRWQREDGNFVSQGGQYDGFG
                     QTLWAYGQHVRMTDDRDFAEKVFPSVLKAIAWLESARKTDPLNLIPVTTPGDNENITG
                     HVTGHNFWTLIGIKNAVFIADYLGEGEKSQQLQKLYDDYYATLMRILAEITAQTGGYI
                     PPGLDGEGG"
     gene            complement(24436..25794)
                     /locus_tag="PLI55_00820"
     CDS             complement(24436..25794)
                     /locus_tag="PLI55_00820"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPA99413.1"
                     /translation="MIKKSLIVYFVVMTIRGIGILQAQAITPDPRSKVDILLVVAHPD
                     DETAIGGQLAKWIFDDGKKIGVIYTNRGQGGGNSIGKEQYHSMGAIREIEARRALSAF
                     GIYNVWFLDGIDTPGQDVLHSLQNLPHGAALEKLIRYVRLTRPEIIVTWLPHFVAGEN
                     HGDHQAAGVFATEAFDMAGDPTIFPAQLAFPREIMDINNYYEGLQPWQVKKIYYFSDR
                     DEALNAPGPQFDISAKSPSQGKPYIELAAQLHKFHQTQADVAQIAEQAEQSGNWQPMI
                     EWLEKFHLIYGKSVVKCEPTGDVFEGVSEKPADFHKNTGYISPVARGVKLQFGGIFDY
                     YRHFWQVHDLQHLATIVSPEIAISVGSYFHVPLLITNGDKKTIRLSVKPILLTGWEEV
                     SGSGEYLIPAGESLPVQCFYFAPNKETDGFQPLKWQIIQDSKVLDEIVMNVKLVEWAL
                     PQ"
     gene            complement(25823..27109)
                     /locus_tag="PLI55_00825"
     CDS             complement(25823..27109)
                     /locus_tag="PLI55_00825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA99414.1"
                     /translation="MAKYFKEPLLINPEVRRNFLLNWMDGTFFAFGMSFVPVMTVLPV
                     FVRQVGGNNITVALVQVIWIIGVNFPQIFIANYTRQLPYKKPFFLKTAALQRLPWLLM
                     ALLCFFIIKNVSSNMGLVFFFIAFGLAAIAGAVNYPGWFDLISKITPVNLRGRLFALR
                     TITGAILGIAGGCLVKIILSAQPFPANFGYLFLLAFVMMTVSYVALIFLKEQEANAST
                     ESLNHFQYFRQLPNLIKSNHNFLNFLIADSLMTIGLIAQPFFIVNAMQRFGLDSASSG
                     SFTMIYMLSMVILNLCSGSIGDRFGHRVNMIIAAFSVLVSCVLAITLRSLSLYYFVFV
                     LSALNTSMIQVSRLPILAEICNEKERPTYISISNMITSPFILLGLLGGYLVDLYGYDV
                     IFAIAGLGGFLSLIWWVFYVAEPRRQNIFVVESVER"
     gene            complement(27093..>27392)
                     /locus_tag="PLI55_00830"
     CDS             complement(27093..>27392)
                     /locus_tag="PLI55_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99415.1"
                     /translation="DFLAGLIKKYANISTNYCDSLGQIRVDLLKNESGQMMLILRNLT
                     ETAINTEIHICGLIPKHLAEQFSAETLNVVYQDNDSSFNITLNPKEVKVYFGEIF"
     assembly_gap    27393..27492
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<27493..29078)
                     /locus_tag="PLI55_00835"
     CDS             complement(<27493..29078)
                     /locus_tag="PLI55_00835"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPA99416.1"
                     /translation="MRNILKESYIPFGSQYYRAPSPSRNEWENDLKNMADNGFNTVKF
                     WVQWRWNQPSEDRFCFDDIDELMDLAERFRLKVMLNTIFDVAPAWIYHKFPDASMVTL
                     SGRKIGPQTQPHRQIGGLGYCFNHDGVMQHFFQFLAETVKRYTNHPALAIWNVGSEPE
                     LTSSMAEMREYADDASKMGDMLCYCDNCRVKFRNWLELKYQTIERLNISWNRNYTSFE
                     EIELPLTRNTFNDIIDWRMFFVATLGENVRKRFEVAKAIDQGRHPLMCHHVFIQGFPV
                     TSTASDPWNVGRFGDLHGFTQMDDPAMIDILRSCAKGKPVISAEMLMLNGYTLDLPRS
                     IDINDVKRFIFSGIAGNLKGFIFWQYRPEILGREAPTWGLTTLDGEKTSWLQDFARVG
                     QVLQKNADFLLEAQPQPAEIAISYNPENQIFIWAATGNEKNATNSLLGAHKALYEHNY
                     NIDFVHPIEIDEKLLSRYKVLIFPFPYLIDQRICHLLEDWVKKGGLLIGESYFAAWNR
                     ENGHHEKIVPGYGLPKIFKAR"
     gene            complement(29113..30039)
                     /locus_tag="PLI55_00840"
     CDS             complement(29113..30039)
                     /locus_tag="PLI55_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004404801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPA99417.1"
                     /translation="MKLGFLTACLPQLGLEEIVKWTAEQGFQALELAAWPVASDRDYQ
                     ARQIDAANFSKQDAERVKELFNKYQIEISAFAYYDNNLHPDLKQREYYHNHLKKVINT
                     ASLLGVELVGTFVGARPDKTPKENIQEIGCVFRELVKYAEDRNVKLMIENCPMENWVK
                     FGLPGNYAYSPELLEALFNEVPSENFGLNLDPSHLYWLGIDYIRVIKDFAPRIFHTHA
                     KDAEILPEGQYKYGIFSRQIDPIPWKSGWWRYRMPGSGKIDWGLFIRTLQEFGYDYVL
                     SIEHEDPVWEGSVEKVETGLKLGLKYLKQYVI"
     gene            complement(30071..>31090)
                     /locus_tag="PLI55_00845"
     CDS             complement(30071..>31090)
                     /locus_tag="PLI55_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPA99418.1"
                     /translation="ADKFGISQVYENWRELVNNPEIDVLHNCTPNYLHFEINKAAILA
                     GKAIISEKPLTNSVAESEELVKLAREKKILTAVNFNYRNYPIIQHARQLIVSGKLGEI
                     FLAHGHYLQDWLIYPEDYNWRVDPEMGGNSRAMADIGSHWCDLVQFITGQRIIEVFAD
                     LVIIHKSRRKPQSDVATFKGKEVTPDSTQCITVKTEDAGAVLFHLEKGGHGVFNVSQV
                     SAGYKNGFDIAIDGSQMSLAWKQEEPNQLWLGYRDKANQILVKDPALLEADSRSFAHY
                     PGGHPEGYPDTFKNLFLNFYTALRTGKLAIHEQNFPTFEAGLQENRIVEAVLKSHQEK
                     RWIQV"
     assembly_gap    31091..31190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31191..>32536)
                     /locus_tag="PLI55_00850"
     CDS             complement(<31191..>32536)
                     /locus_tag="PLI55_00850"
                     /inference="COORDINATES: protein motif:HMM:NF039707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HPA99419.1"
                     /translation="QFTCQVHCTFTQSGLRFQAKYSAPEPIWLVEWSLTNLNLAEVIL
                     PALGGQMITEQMPDQTTLSYKYPFWLNAQFVIGIKGNRGLIIYSHDSSSNLKLARVRR
                     ENDSFTISYGFEPPAKKRLTSFETEWFIEEFTGDWQKPVAEYRNWMEKAFKIKPLPQK
                     QNFPNWAKNINFILEIWGARRESQKPHHTFEQMIERLNEFKNLHDPRRTLLYLPGFAE
                     HGIDSHIPDYNPSDLLGGEEKFKELVDTAHLLGFKVMIHTNGLGMTYTHPKYPEFKKY
                     QVVDAFGRLQGWAMDIDGDWLTEPYFAYINPGARAWSDLMIETLGRLIKEFQIDAVFL
                     DQTLLAFNVKRGPDFIKGMRTHIERLQKAFPEILFAGEGLHEQVAGCLPVAQIHGIDS
                     LSGVHGIEGQEPWRKVHPVSTYLFGNYSKYVAHLLTRHPSNPTFKLQESAYSQLNV"
     assembly_gap    32537..32636
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(32859..33335)
                     /locus_tag="PLI55_00855"
     CDS             complement(32859..33335)
                     /locus_tag="PLI55_00855"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF016040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050385 - ureidoglycolate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HPA99420.1"
                     /translation="MNTLTKKIIPVNPVNFAKFGQVVALPREKPTAEGDTFKFWANIA
                     NYRINGKTEIGLCTVTLPRGNRVNSLECHLHTPEILIPVDAPFALPLLIEGEPEENLK
                     IFRVNVGEAVVINPGVWHGACIPLEKSEATYFVIFRYQTSQKDIEKKSINDIEILI"
     gene            complement(33340..34785)
                     /locus_tag="PLI55_00860"
     CDS             complement(33340..34785)
                     /locus_tag="PLI55_00860"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPA99421.1"
                     /translation="MKKYYCLQSFLALIMLIIVDCSRPNSPNYQRLMKTKDAEFVHIP
                     VGLCEDYPEETTTMEIIYNDMELLKRTGVDLLRISFGWDAIEAQKDQYDWLFWDDYVK
                     TAVNQYGITLIPYICYTPLWNSRGDTLWFWNDQPLDFEEFGEFMDDLVTRYKKYIKTW
                     ELWNEPDLDIYWDTRDVAAFAEFIKIGAQAVRRADPEAKVVLGGMAYHADFLERLFRD
                     YGLSPYIDIVNMHNYYETWSERPVEKITENINAMAEVIKKYGDDQSLWMAEVGYSTFR
                     QGSYVSDSYYAYYDYEHTPEYQAVDLFKRLTLAVAEEKLSAIAWYEIKDLPAGEEVIG
                     DNYNNRYLGIAYADYRPKPAEKALVFFRKLFGNPYRNVTSLISVEHQPDSDVFYRAFE
                     KEDGTIIFVAWIATNVPSKRPLKPANLVQDTRTETISVTIPAKLKGRVVQYNELGSAS
                     TFNEFQRNDDQTKLNNIVLNGGEIKIFELEK"
     gene            complement(34884..35285)
                     /gene="rbsD"
                     /locus_tag="PLI55_00865"
     CDS             complement(34884..35285)
                     /gene="rbsD"
                     /locus_tag="PLI55_00865"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HPA99422.1"
                     /translation="MKKHGILNSHLSMVIASLGHTDQLVICDAGLPLPREAEIVDLAL
                     VPNVPRFIDTLKAVLEELIVEKAILAKEIESINNGILKEVKDLLKGVPIEYVSHEEFK
                     NIYRESDSAVFVRSGETSPYANIILISGVDF"
     gene            complement(35302..36222)
                     /gene="rbsK"
                     /locus_tag="PLI55_00870"
     CDS             complement(35302..36222)
                     /gene="rbsK"
                     /locus_tag="PLI55_00870"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HPA99423.1"
                     /translation="MTKNSVIIVGSANMDLVAQVDRYPQPGETVFGKKFGIYPGGKGA
                     NQAVACAKLGADTIFIGKMGDDIFRDRLVNNMESNGVHMEEIFIDSETPTGVALIYVD
                     ADGQNEIVVVSGSNMRLTESDIRSKEKLFERAKIVLCQLEIPLSAVEAAAILAKKNGA
                     VFILNPAPACKLPASLLNGIDFLIPNEIELSFLTGQSVTDRASVISSSRKLINTGVGN
                     VITTRGENGAILVNSDSWQEFPATPVKAVDTTAAGDAFNGAFAAALASGKPVNEAIPF
                     ANKVAAWSVTKQGAQTSMPTLADISNYLNQ"
     gene            complement(36233..39496)
                     /locus_tag="PLI55_00875"
     CDS             complement(36233..39496)
                     /locus_tag="PLI55_00875"
                     /inference="COORDINATES: protein motif:HMM:NF028442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HPA99424.1"
                     /translation="MKLLNELWPDKELNCENFKNPPLEMGILPFWFWNGEMDEAEMEW
                     QLKEYKAKGITGLFIHGRFGLKIPYLSEKWFERVKFAVKKGREIGLNIWIYDEMNWPS
                     GTAERQVIKKYPYLSQKYLEMVTLFINGPLFIYLEATDSRYINTGDSKPIAAYICSAE
                     EYRTGIKNIIDVTPNLSFNKVIPWEAPAGNWVLMYFLEKEIDYYIDALNPEATKRFIE
                     LTHEQYKTAVGAEFGKVVPGFYTDEPAMHYFQVGTDNYVIPWSQRMFKIFRERNGYSI
                     KPHLAALYANVGRDTARIRYDFWKTLSDQYDDSYYKQLRDWCAENGVLFTGHILFENW
                     LRGHARCEGNVMQHLSNLHLTGVDHLYPKIGSEEQPGEHVELKMASSITHHLGKKRLL
                     CESMGGTYWDCTLERMKWIANWEYSLGVNLFNNHGYHYSIEGERKRDWPPSQFYHHTW
                     WKYYNEFTRYMARLGHALTGGKHIAKILILYPITSIWANYVPQAPDDISRLIEMDFNY
                     LTDALLHLHYDFDYVDEDILINAQLNGNRLEIADESYELIILPPVTHIKKATLDKLKA
                     FVNNGGKLIADILLPVEQFIDGKPSKSRAVDELFGVKPNCILADFQNGKNAVNQLKML
                     QPGVCLIQGGGLAKDRRRQDIKEAIESCISADVQIGHSEVFYLHRVKDGRHLFYFVNT
                     LQQDIGEVDISLETVGRPELWDPATGAVKELSVFDIINNRLCFRLTFGASQTFLVILH
                     NDLPDSYISATNLVLEKTEANFITGYIKNGDLAKLTLKTGGKQKDIVQLVPKNPPPLI
                     LPEVMNFKIENDNTLCLAKWKMLVSNEFSNDNYAALDYDDDHWLPVTIGAWEMQLPEE
                     PDDIHYPQTLWYRIPFQIEELPNRVKLLIDGFSGSSFRLFINGTEVKDTGQRSQLDAE
                     IREINIHSYVHPGRNLIAVQLVVQRRTDGILDLIKITGDFALKLNDDGYSIVLPKKEV
                     NIGDWCQQGYPYFSGTGTYSTELTIPADFCKGKLFLDIECGEDVLELQINDSGPIIVP
                     WHPYQIDITDLVRPGKNTFRFSITNTLINTLEAVPKSSGLLKPPVITNYQLLKIKID"
     gene            complement(39726..40724)
                     /locus_tag="PLI55_00880"
     CDS             complement(39726..40724)
                     /locus_tag="PLI55_00880"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPA99425.1"
                     /translation="MLHNRKIKIVTLSAMIFFATNLRVEGFNKAGRTSLQFLKIGIGA
                     RSSAMGEACIANLNSIDAVFWNPAAITQMQRLESSFSYTKWIGDLNVTAGAVGLTIDR
                     LGSIALDYIAMDYGDIPEALVTSPDGKLDTRTGLNFSGGDLSVGLSFSKKFTDRLSIG
                     IRTKYLREDLYTYSTSLWAFDVGTYYNTGWKGIRLAMTAQNFASQARWLKTQQREEQQ
                     YEVPLVFRIGTSIDLLGGKDLFLGGNPNKQRLSLNIDAIHSNDYAERLNIGAEYWLWN
                     RIALRTGYRTNREEGHFSVGAALNIPMDFTNLVVDYSFVDYDYLNSAHQFTVMLRF"
     gene            complement(40769..42976)
                     /locus_tag="PLI55_00885"
     CDS             complement(40769..42976)
                     /locus_tag="PLI55_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99426.1"
                     /translation="MKVKNYYIHLVIILGLLTGWSESFAQRKSTHTRGKLWETLNNVG
                     LIGDLGVWDYEETSGLGFYPGFSGYSFPNNEQLANGWVTDANFHNFRSGPALVIKGGD
                     VLVAPDYDPQYQEYLFYHSSFAGGSHGILWNYPPFIKTNNFVGTSQFNPLLPEEINYC
                     EFPTATGISVKMRSMAWSFPDYDDFIVYDYVFINTGEMVIPALGEVKHYEQTFDSVFV
                     VFHSGIQVSTKGHLNFHYDPDFMSSAAPAGGFGGWKQLENGGTYTDYFAVENDGDDSK
                     GLFFYSRDFNGGREPHPNDTYSKRSDWQTRLKYNQSYENIELQDPACFGFVFLYRTPP
                     TDSDADPFEADPNYFNIYSDEGEQFQGRALDMNEYFSIERLGEKGMFDFITHNRIPEE
                     NNGDMYCFYTATFGPYYLAPGDSVRLILAEVAGSLDYHEVIRGDPNHYYPDSSIADIR
                     RNAEAARRALKWGFGATVEGINLAADVPESPPAPNCSATNASQGSDTAIIAIQWDKLA
                     EETVITDGAGNPFYDGSTDLSGYRIFRGIDKRGIWDEVVDIPIEEANRYWNAEIGKYV
                     YLDKDLQFGFDFRYYVQAYYSNPKTWVSANHTIVDNLGELVSDDYNMTPLTGARPGPV
                     DITAGWDVFVAPNPYIEGDPERSFGDPTPYKIEFRNLPERATIKIFTVSGDLVKTIEH
                     GPDTYGNLFGSVAWDQRTDSGLRVAPGLYIYAIESHAEGTKGKRTIGKLMIIR"
     gene            complement(42994..45861)
                     /locus_tag="PLI55_00890"
     CDS             complement(42994..45861)
                     /locus_tag="PLI55_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA99427.1"
                     /translation="MISKKAYLQIRRLISRTFLGFMLLISLGYAASDGKIVGKVIDKK
                     TGNPLPGVNIIIVGTEMGAATDMEGDYYIINVAPGIYSLQASMIGYKQVIKTEVNVSV
                     NHTTRVDFELEETVLEFGEAVTVVAERPLIELDKTSSRVVVEETDIKSRPVSQLSDIL
                     TSMPGIDYNPYGELTVRMGTLDQVAFMIDGMRARNPLNSQPYFNVNLMAIEELEIITG
                     GFDAEYGEAQSGVFNIITKEGPDHYVGAVEFRWIPPGLYHWGTALYDYSTERYWENTH
                     ARHLQWWIDNPDMWVDPNGRYGNDPSCSWTPEQAYQFYIDTHQPLTDYTNRSGYQTEF
                     SFGGPTPIKNLRFFLSGRYRTAPPITGNAYRKMGSWLDGTYKISYSLGGNKLLVFSGY
                     YNQDNTCHGMESMEYEGDYGNKYAYYDEAGYPVYRRNGQTLKFVHNLDKNTFYELHLS
                     RVFNYESQTKFPSDTADWTTSDIRRDYLRAYDSQGFAIPDAWAEIYGLHYIGYWYRKE
                     DRNTDLTLTGKLTRQINKNWQMRSGFGFTYYTFDRYQEARAWEAVEDKIYHPYEGNIW
                     WSNKLEFEGLIMNLGLRYDFYNPNDVIYLDPYDPFDIITAEMEGREPNPKTKPTYTFG
                     QLSPRIGVSHPITEKSLLHFNYGHFFQRASFGEYGEGEQTYSILSTYIVDPEFGSIEP
                     RNLGNRELRPRKTVAYELGIEQDFGPVLADVTAFYKDRTNDVRSITVITYNEQQYHTS
                     GNSDYADAKGVEFTLRRPLRGFWGGYLTYTWETGIVGQSGDPFTIYPPNVTVHASQDF
                     DIGDQLYYMRPSLSANLTLAAPSNFSPLGGVLANTQFSIDYYVSYPHKQISSDVYQGA
                     VNIIRPANKNANIRVRKEFKGFKGTRPAIFIEMQNAFNDKWHNVDLMEGDISAEDQVR
                     YINSNLMVYPEKMTNGAPFPDYAKYTNLPRKITVGMALSFQ"
     gene            complement(45930..46880)
                     /locus_tag="PLI55_00895"
     CDS             complement(45930..46880)
                     /locus_tag="PLI55_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPA99428.1"
                     /translation="MRIAALYGAHDFRIETKESPDIKDDECLVRVRACGVCHSEIHQW
                     AKTLDNLEYPRYIGHEVSGEILKCGSAVKGFKRGDRVAVWTDRAGYAEEVAVRANQIF
                     PIADQISFAEAMAEPIACTTNGVIKAGVELGDTVVLVGTGFMGLILMQQIALKGASRI
                     IGVDVRAEILDKAKMLGADLVINPQEEDVPAIIWDMTNGRGADVVFEVGGNQASLDLA
                     VDLLRMEGKLVIFGYHPGQRIIKDLGYWNWMAFNIVNAHFRDMATILRGAEIGIKLLN
                     EQKINMKPLITHKFKLDEIEAAFCSAQEKPKGFIKAVIEP"
     gene            complement(46970..>48141)
                     /locus_tag="PLI55_00900"
     CDS             complement(46970..>48141)
                     /locus_tag="PLI55_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPA99429.1"
                     /translation="WCATYIQQNSEYWALLNFLPGPDDVIVRLELNVPKVSPADVVID
                     GVMDESVWGNAAEVNLVTETGYNIWASSYGREIAEPEYDELYGRLLWTDDTLYVFMHI
                     DEFVNDTTDLYWGGQFLDPTDTSGHWASDQLFIGVSNRLGVESWDNWEGNPMAAPDGP
                     YHLMIMGDRVTFNDGYPVYVPELDSAVIFDPSTFIRSAAQWNKETGVWDVELAIYNPN
                     VKASASVGFNVGGSFSSTYSRETTGDSYGYYTWQPNVPNEPFADPGDLGWCATYIQQN
                     SEYWALLNFGTGLNGIDVAEVSEKPVRFALRQNYPNPFNPTTTIRFEVNERVPITLKV
                     YNVLGKEVATLIDNRTYSPGVYSVIWDGSSLSSGIYFYQLVTDKVVSTRKMILMK"
     assembly_gap    48142..48241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<48242..48497)
                     /locus_tag="PLI55_00905"
     CDS             complement(<48242..48497)
                     /locus_tag="PLI55_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99430.1"
                     /translation="MRKVKKQMKGGVIYKSGLLYFCKTGQGIINQFINQMEVSMRKVN
                     MCLLALLLLVAVQSVALSQEEIVRLELNVPKVSPADVVIDG"
     gene            complement(48494..49795)
                     /locus_tag="PLI55_00910"
     CDS             complement(48494..49795)
                     /locus_tag="PLI55_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HPA99431.1"
                     /translation="MRKKIIFWTISMVLILSDFTKAGDASTAPITSAKSLALGGYNYA
                     GLDGITTTFQNPGKILSTPGFGLAMNIIDRAGQQQCQKGSRDIHRSYLFNDFSGSAGI
                     YWVPNQKFGLAMVYNRALDYSVRWPLALMFTENGITRLYGFQLKSRLIADAYSQVFGL
                     QVGKMSLGAAINIYQIYQQFEFPISNPEWQTNRTNPVYALNLEQEDNAIGWSLGFILP
                     ITTTIKVGGSIRSGVDFDLEGNAKTDCFYALDSLAKKSRLHSRFQMPMTGGIGILYKP
                     TDQLNLNIDLSSEFWGSTQSKLDFNYSDTAWSAIIAAAPRDSITGYWRSGMPFYDHNS
                     YVFAIGLEYSGENNLKYRVGYNYQTTPNSDHSYSLLLPNVNQHRISAGIGFLFNQFYL
                     DLSIAYSVGVAKDIKKSQDEYFYGKYDTKTIIPSINVEYQL"
     gene            complement(49829..50794)
                     /locus_tag="PLI55_00915"
     CDS             complement(49829..50794)
                     /locus_tag="PLI55_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99432.1"
                     /translation="MNYLFKPNHFISASSILILFLLCLLRYCGEKNTLAPYQGARPMN
                     NIIIEQETFTPKFTWLGGYGCAFGVNRGDQARLDSTLQWLIKVDGDNLHYPVKYGELP
                     EGVTDITNEYGGHFEALIEDSTYTFWIVKEAAWDAVVENAGKVILMAELDTVTTIANE
                     DTLWIDSYSHVQKSCWIDVYTNVDMASIKLRGGFAKITLIETDTSNSPVITWEIIDSS
                     VEDSLVAAVGICLGESYSPKKIVWEAWSVDTVGGQKVYGKNNVIGQPLVIGSPIPGTK
                     VFVEYPAKGLSRNKTYLFWLAGKDWNGVDHLRSTKYHSYITFNTW"
     gene            complement(50855..51697)
                     /locus_tag="PLI55_00920"
     CDS             complement(50855..51697)
                     /locus_tag="PLI55_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPA99433.1"
                     /translation="MDIDFRKSSHIVKDIILHVIIYALALATIAPFIWMLLTSVKDIG
                     DIFVYPPKWLPSKFHFENYARAFQAAPFGRYYLNSLIVACTVTLGQLITCSMAAYAFA
                     RLQFKGRDVLFYIFLGTMMIPYQVTMIPSFMVLYWLGWVDSYQAMIVPGLASAFGTFL
                     LRQFFLTIPKELEEAAYIDGCGKFRVLWQIIIPLSKPALATLAIFTFMGTFNDFIWAL
                     IVVNSDHLRTVQLGLAIFRDRYITDWDLLMAGSVMATLPILIVFFFAQKYFIKGITLS
                     GIKG"
     gene            complement(51697..52629)
                     /locus_tag="PLI55_00925"
     CDS             complement(51697..52629)
                     /locus_tag="PLI55_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPA99434.1"
                     /translation="MLNKSTRVKKTIINRRDGNSKKNILTALGFLSPTLVIFCAFVLF
                     PVFFSFYLSFHKWNMFSSGREFLGLENYRRLFSDPEFWMVLKNTFIYTLGTVPLNMVI
                     ALLTAIILNRRIAGKRFLRTAIFTPVVISPVAAALIWRWIYDPNFGLLNYLMDLVGLP
                     SVNWLNEPKAAMVALIIMGVWKTFGTNMVLFSAGLQGIPDHYYEAAEIDGAGWWSKFW
                     NITMPMLSPTTFFIMIMSMISSFQVFDIVYVLTSGGPLGATKVMVFYIYENAFKFFEM
                     GYASAAAYVFFAILAVLTVLQVKFMQTRAGYEAV"
     gene            complement(52619..54010)
                     /locus_tag="PLI55_00930"
     CDS             complement(52619..54010)
                     /locus_tag="PLI55_00930"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPA99435.1"
                     /translation="MQFKNIGNQHSPQKCANVFTANLRGWSRYFLLSFCLLVFIGCGK
                     KEATKDDKITITFWHSFVASTIPALEDLIEQYETENPNVKIKAQYVPTGDALIQKLIT
                     AIQSKTAPDISWIHSDFLADLVDADAIYEMDHFIKSNNGLSKSEIEDIYPALLQYSSL
                     RGKIYCIPMEATNLALIYNVDMVKEAGYQDGRAPSNWEELEEYAVRLSKDFNNDGKFD
                     RIGFFLPIFPAAGPLSGWMVWQWIPYLWQAGGYIIAEDQSKVLYNSEAGVKALTLWKN
                     IYDKLNLRTFTTDYDVSFASKLVAMAMDGPWNLPRFNRMLKNLNWSFAPLPKGPVKQA
                     TICGGEYLVIFKQSEHPDEAWSFVKWIIRPDIQARWAMKSGYLPVRRSVSEIPEFKKY
                     LIENPNFKVFVDQMEVAQIQRSMDYGALQITRHMAEAIEEATLGGGDPKAALDKAAAK
                     SNKVLQSYWQNAK"
     gene            complement(54036..55070)
                     /locus_tag="PLI55_00935"
     CDS             complement(54036..55070)
                     /locus_tag="PLI55_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPA99436.1"
                     /translation="MTATLEDVAKKSGYSVSTVSRVLNNKGKVNRIKDETADKIHKIA
                     QELNYVPNQLARGLRLRKTHAIGLVIPDISNPFFAHVSRIIQIESYKLGYTLIVCNTD
                     ENQDTEIEQIQLLRSKGVDGFIIMPVGLKSDHIQELIDDKIPLVLLDRCFDSIDTNSV
                     VVDNYTGSYKAVEYLIKNGHKRIAIIQGLPNTSTNNMRVKGYKNALIKYNIEIDENLI
                     VGNDYRRENGYIETKLLLKMSNPPTAIFSTSDLITLGTLDAIIEEKLNVPQDVSLISF
                     DDVDFAPYLFTPLTAIQQPRQIMGEMAVKLLYDDLKSKGRKEKTRIELKPVLNIRKSV
                     RNLNSVINPD"
BASE COUNT        16284 a        12720 c         9838 g        15930 t
ORIGIN      
        1 gtcttactac aatacttcaa ccgctccgcg gtgtgggcta aatcttacaa caactcgttc
       61 aagattagct atagatgata gtcttacgag agccttataa taccttattt ataaattttc
      121 tgcacgtgac ttcagattat tgatagacac gatttttcgt ttatcgaatc gctttttctg
      181 ttatttgatc gaatatatga actttatcta tgttgaaaaa aacaggtatg gatgctccga
      241 ttgatagagg catattggtt tcctgagtgc gaagagtaat ttgatgcccc tcgtcggaga
      301 aatacaaaat tgattcattc cccagcggtt cgacaacttc aagagttaac cgcacttcct
      361 gatagtgagc atcttgctta ttcaagtgga catcctccgg tctaatgccc aatactaccg
      421 ctacattatc catttcctta taaggagatt tctccaagat aagtttgata ttattggtgt
      481 tactctggaa aactccctga tgtaaaacac ctggaatgaa attcattgcg ggactaccga
      541 tgaagccggc aacgaataaa tcttgcggat tctggtaaat ttccgaaggt gaaccgatct
      601 gatgaatttc tccatccttc atcacgacaa ttcgatcacc catagttaaa gcttccacct
      661 gatcgtgagt tacatagacc atagtcgttt ttagatgatg gtgtagtttt tttatctcgg
      721 tgcgcatttg gactcgcaat tttgcatcca gattcgagag tggctcgtcg aataaaaata
      781 cttttggttt ccggacaatt gcccgaccga gagctactcg ctggcgttga ccgcccgaaa
      841 gttgtttcgg tttccgattt aacaaatcgc ctaactccaa aatatcagcc gcttccttaa
      901 cccgctgtcg gatttccgat ttaggatatt tacgcagttt caagccgaac gccatattct
      961 cgtaaaccgt catatgaggg taaagcgcat agttctggaa aaccatcgca atatcgcgat
     1021 ctttggggag aatattatta accagacgat tgtcaatata aacttcacct ctggttactt
     1081 cttccagacc ggcgatcaag cgcaaagtgg tagatttgcc acatccggaa ggtccgacaa
     1141 tcactaaaaa ctcaccatca ttaatcgtca aatcgagatt tttaatgatt ggagcacctt
     1201 tatcaaattc ctttacaatt tgttttaatt ctatagaagc caacgttttc cctttctcgt
     1261 tttataaatt aactaacgtt caaatttact acacaatatt tcacaatgtt cctttgacta
     1321 ttaatcccga ttacattttc aagtaaaatt ttacacttcg ttaatattct tgtccgattg
     1381 taatttaaat ttcgataaat actcagccag acgttcacct aagcgcaagc aggcggcttt
     1441 ctccgcggct gaacgcggtt caccggccag agctactcca taatgaagtg tcaactgttc
     1501 atctacgtag tcggtaacgc cgaaaaccag gaatccgaaa ttgatgagaa tcatcaaagc
     1561 cgattggcag gtaatctcgg cgccgccacc ccaacctcca gatgacgaaa atgcgcaacc
     1621 gattttgcct tcaatttttc cccagagttc atcacctaag tcatcccaaa attttttcat
     1681 tttccaagat actattccca tattagtcgg cgatccgagg gcaatgccat cacaccaacg
     1741 aatatcttcc gccttagcat catcaatacg taaaatctta acatcgtgat caccatttgc
     1801 cctggcaccg agggcaacca actcagccat cttagccgta ttaccagttt tagaatcata
     1861 cagtattaga atattgctca tatttttctc ccgattttaa gttaatttct ataaagtaac
     1921 tttcagattc atcagccttt ccttttactc cgtttccgat gataataggc gtctgatttt
     1981 ctcatcagcc acttttagcg atttttctgg agctattcta ccaatgatac tactttccag
     2041 cgcttcggcg atacattgat tgacttcaac ccgaaattta tccggcaaac tacgtcctcg
     2101 agcataccga atctgttcat tgaaagattt ttgctgagga aattgttcca aaaaattaac
     2161 ataatcagaa tcattcaata ctgaacgtcg aactggcaga tagccagatt tttcagcaaa
     2221 agttcgttga acttccggct tgagaaacca attgataaaa cgccaagccg cttccggatt
     2281 gacacattgc ttaaatacca ccaaactttc tccagctaaa taactggctc gtcccgctga
     2341 accggcaggt agcggcgcca cgccccaatc aaatttaaca tattgacgat agcgtggtaa
     2401 attccaaggt ccatccataa tcatcgctac cgtctgactt ataaaaccca tatcgtgtgc
     2461 catcgaaaag cgctcgaatt tttgctgatg ataaattttg ctccagaagg caagcgccgc
     2521 cacgcctgcc tcgctggcaa atgttggttg attattacta aaaatctcgc cacccgcttg
     2581 ccaaacaaaa ggcgcccact gcatcgtcat ccagatattg agcggaccag aagccggaaa
     2641 aacaggaaca taaaatccgt agcgctccgg ctgtccatca ctattagaat cgctacacag
     2701 ttgcattgta aaactgtcca gttccgccca tgtcgcgggg ggatgatcgg gatttaaacc
     2761 gatctgtcga aaccaatcgc gattgtacaa aagggctaac gtcgtggctt ccataggcag
     2821 agcaaataaa gtatcccgaa aggttatcgg cggtaatagc tgcggaaata tatcaggaag
     2881 acactggttg taaaataatg aatcgtcacc agcaagttca tttatcgaga aaattgctcg
     2941 actattaacc aaattatcca gaaaatcgga atgaacccag gcaatatcag gtgccgtttg
     3001 gctttgaatc gccgtaataa gtttttgcac cagcgcatct ccggtcggaa catactgggc
     3061 ttgaattttg acacctggat tttcttcttc aaagtcggca atcatcgcct ccaatatcgg
     3121 taaagtagta gccacgaagc tatgccaaaa ggtcaatgta atgctattgg catctctcgg
     3181 tttcggcttt gaacaattat tagccataac aatcaaaaaa acaaaaccga cgatagacaa
     3241 tcttttaatt tttctatttt tcacgataca aatgccccgg acgttttttg cctttcatac
     3301 taccttcaaa ccagacagcc agaatgggtt cttggacaat tggaaagtcg tgatgttcac
     3361 cggctggggt aattacgcaa tcaccggctt gaacagcata gctttcccct tccgaaacta
     3421 ccataccttt acctttgata atgaaccagt attcatcaca atcgtggaaa tgattatcaa
     3481 atacggtgtt tcgttcatac tcgaccggat cgccgtcgtt acgcggatgc tcactttttc
     3541 ccaaccggaa gaccccacaa ctcccccgat cgttactcca attgccacta atttgaaaca
     3601 attctaccag cggtttgggt tcaatccaca cctcaccaaa aaattgatcc aaaacatccc
     3661 ctgaaaatat cgtccttttt tcggctcctt cgctaatttc agccacacca tccaaaacga
     3721 acagcaatgt ttcggaaaac tctgatggga gctgtagttt ttgcctttta gacaaaccga
     3781 ttattttaca gcttttgact tccgaccaat ccggaaacgg ttcattttta taggatatta
     3841 caggcattag gactttttgg gtatttagtt aacatcggaa catccgggtt tatatccggc
     3901 ccgaaaaatt tatagataat taaatcgcgg tcaccggtat tgacgacttc gatcgggtaa
     3961 atagcccgct gatggcaaat caacagttca tctttttcaa aattaagacc ctctatttcg
     4021 acatttccgt aagttccctg accccgccaa gccaaaatat tatacactcc tttctcaact
     4081 gaaacgtgtt ttccccccgg acggataatc agcttcttgc cgctaaactt atgagtatta
     4141 taaaaaatcc aggactgttc acttccagat tgctggtcag aatcaatgag tattggtggt
     4201 gtatgccggt tttcataaaa gtaaggatcg ccatttaatt cccaatcaat catacctaga
     4261 atagcttttt ccgccaattt tttccgttct ttttctgaaa catccttaaa tagtaattct
     4321 ttatcaataa taatatcgcc accaactttt gcctgcatca ttgcgaacac atcggaatct
     4381 tcctgtaatt caatagtcaa gaccgagcca ggtgcgtgta gaattcccgc cggaacatga
     4441 aatccatcgc ctgcctttaa acggtaggca cgggaatgga gaagaatgct atcatctttc
     4501 cactcctgca tagaaggtaa aagtaattca tagcgctttt ccagactaat ataaggatga
     4561 acaccaaaat aggtttccgg ctcaggtccc agcggtacct cttcggggaa ataataggct
     4621 tcttccttac tattgcgacc gactttgcga gcatcttttg ccatctggtg taaatgataa
     4681 ggcaagcgat aagcatagtc gaaaattttc ggcaagcgtc ccagcccggt atgagcctta
     4741 gcataatcag ctcccataat caatgccggg gctatctcaa ctgccgcttt tagagtaatt
     4801 tcctgaccat cttcaagatc caaacagcat aatccttcat tgggtacctt aatacgatta
     4861 tcagccggtg tagtcgaacc tacccagcgt tcgcaaatac cgccgcgctc accgagatca
     4921 tacatttcct ccggcaatcc caagcgccta cccggcggta aaaaatccct agcaacccaa
     4981 gccggtttaa gacgcaaaat accgtcatta tgttcaatta gcttttctaa ttttgaaata
     5041 atttcattca taaattcacc tctaaataat tattatttcc aatttatcgt tggttgacaa
     5101 tcctcgcttt gaagataagc aactaactca tctcttaatg tcgtcgtaaa aggatcatct
     5161 ccataattct gaattagatt aaaggtcgtt aggatcaatt tccctgagcc aacccgcatt
     5221 tgcattaaat aagcggaatt caaatggagc cagccgacat acattccggc taatacatca
     5281 acaaactgct ccggttgaat cccggtgatg gcatatttag gtaatgcctt ataggcttca
     5341 aagcccagca ctttattact ttttaaatta ttaaatggcc tgtgactatt gtcaacccaa
     5401 ttcaaattcg aagcccaatt gccgtcatac ccatccgatt cccgtgacgt aattgtaaaa
     5461 ggaaaatcag ccggtaaaac tgagctagaa tcaatgacgc atattacatc caagccattt
     5521 ttcaaatgat ccattgtttt tagatcaata gaattacata tcaaaagatt agaattcggc
     5581 gaaatattct ctatctttcg cgatggatat accgtaaact cgataaaatt ctccgctaag
     5641 acatcccctt ccctgctcaa taaacggaat ttgacacgtt gagataccgg ttgttttacc
     5701 ggctctgaat taattttcat acttccgaca aaatatacac cgaccgaatc aatcgcaata
     5761 ctcttgtcac cgataatctc accattgtaa atcccctgga tacgaacatt agaaaaatct
     5821 ctattactat aatgtgaaat agataattgc acgtctatct cttcattatc ccaaaatcca
     5881 tacttggtta gtcgaggaat tataatgtca ggctgttgaa cttgggcaag tatctggcga
     5941 tcggctttaa aattgcgcca catatccagt aaaccgttac attcccagtt aatgtcggta
     6001 aattcagtga ttacgtaacc ttgcaattca ggttggagcc gaatttcttc gatttcccac
     6061 ttcaacgcct gtgcttgggc tttctgactg aattccgcta attcgttata gtttttgaac
     6121 attcgtccgt attgataatc tccaaatcgt tgagtaacac ctgccggcaa tgttaccaga
     6181 ttatctaaaa attggcggtc gaaccagaac ggcaattttt tcggcagttt gggcaaaccc
     6241 caattgccaa attccgagat catcagaact tcctgcccgg tttcttccgc atcgccatag
     6301 ggactaaaaa gccaccgtgg gcggtcgcta acttcctgga ctgtttctct aaacttctga
     6361 tgattttcgg gaatcgccca ataggtatgg tagtcattaa tatcggtttt caggtgatag
     6421 ttaccccagc aggcgctatt atcaactatt agccgaccat tggcaaggga tttagcaaag
     6481 tcaaattcag ctttgagcca ttgccgctga ttggcttgat taaggtcgag tccccagctt
     6541 tcgttaatca gactgataat cgcgagtgat ggatgattcc aatcacgctg aagcatttcc
     6601 tgcaatgttt ctcgggaacg ggctttgacg ctctcatcga agacatccca gttgggaatt
     6661 tcataccaga ctaataaacc caattcatcc gccaccttta gataacgcgg atcaggaact
     6721 ttaatgtgac accgaagggt attcaatcca agttcgcggg ctttttgcat ctcatcgtat
     6781 aaatactctt cagacggcgt ctcataaacg gtttccggat agaaatcctg atccagcgcg
     6841 gcgatcatat aaaaaggttt accattaaga taaaattttc catcacgcac nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gactgcttgc cagatgccgc tggtttggac
     7021 ataccagttt tgtttgccgt gcggtatatg ccagtagcta ataccatcgg taccgacttc
     7081 gtcagccaca ggatcaacga ctcgtactag gatttcattt tctccctgtt ttaatcgctc
     7141 ggtaatgtca aaactaaatg gcgtataacc gccttcatgt ccacctacta attctcggtt
     7201 gacatatacc ttagcaaggt aatccactgc tccgaagttt agcagaaagc gatgtttggg
     7261 aggaagcgat tttaatttga acgacttgcg ataccagaca actccctgat aatctaacaa
     7321 gtccacatgc aagcgatgcc aagaaccggg tatttcggtc atgcgccaat tttcgatcag
     7381 atcaatcgaa ttaatcgtaa tttcgccggt gctatccggc gctaattgcc accgaccgtt
     7441 taaatcaaca accggatttt tatcgcagga agtaatcata attaatcccg ctaaaaatgc
     7501 tgtaattaaa ctccatctgc tcatattaaa aattttagtt atttgattaa tgctatttac
     7561 tccaactacg actaaaagtt agctctaaaa ctcgattgga atgcttttcc gacatacact
     7621 aaactttcca tcttatttca ataacaacat tttcccgacc agaatttggt ctttatattt
     7681 caaacgataa aaatagcaac cgctcgccaa ttggtcggct cggattagta cctgatgttc
     7741 accgggattt tgttcactgt ccaccaattc catcacctct cgccctaaca aatccagaac
     7801 ggtgagtttg acattactct tttgaggaat cgtatatccg atcgtcgttg aagaattgaa
     7861 gggattagga taattttgga aaagtgtgaa tttatcagca attggtgggt gctcttccgg
     7921 tgcaatatta acgttgacca ttgaatcagc taataaaaac aaagaggtcg tatagccggg
     7981 aatagtcaga gccacactac ttagattttc cggtttccca gaaaaggtca ttcccgtaat
     8041 taaatctgaa aggtaatagg ttttcgtaga atcaagatgc aattcctcaa tcggcaagga
     8101 gatggtaata tttttaggct cactcatcat attgataacg ctgaacaaat tctcatctcc
     8161 actacgacga tggaacgcga aagcatagta agccgggact accgggattt ctttaaaata
     8221 atcactgcgg agcgctgaat acctttgcct gatttgaatt aaatnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnntcttta aaataatcac tgcggagcgc tgaatacctt
     8401 tgcctgattt gaattaaatg gcgataatga tcgaaaagtc catatttatc ctgactacgg
     8461 attgatttgc ctcgttgaaa aatttggtaa ctactataag gatgttgatt gtagccgatt
     8521 tcctgaccat tataaagcat cggcatccca ttcaaagcaa aaatcagcgt tgaaaccatt
     8581 ttagtctgag ccaaattatg atttttgata aagggaatca tatcattatt gcccatataa
     8641 cggagaatct tggcgttggc ggcatagcct tgcccgtaat tagtcaatgc ctggcgcaaa
     8701 agattaactc tttccgatgg attaccgtaa ttgaaaatcg tatattgttt ggtggcgtct
     8761 tccgaatagt caacttccca ataccactgc gaaacccaat cttcagtagg cgcccaatca
     8821 aaggcgacat caaaacgttg atcaaaaacc attgaccagg aagctttatc ttctgccaat
     8881 agcataactt ccggcttgat gcgctttaat tccaatcgta agttttgggt aaattctggg
     8941 caacgtgcat ttacacccca cactgcatca aagcgatagc catcaacatc aaatttttcc
     9001 acccaatatt tacaaaccgt catcatccag ttacggactt caggattatc atagttcagg
     9061 ttaggcaatt catcccagaa ataattgata aaccccttat aataattata atattgagaa
     9121 tagggcgcat cgtctatctt cctttgataa taatcgtaat aatgggaagt gctaccatat
     9181 tcaatactgt gctgggcata cggatgataa atagacgtgt ggttgatgac aatatctaga
     9241 agaaccttca tctccagcgt gtgagcggac ttgatcaaat tctgcaagtc actttccgaa
     9301 ccataatccg aacgtatatt aaaataatca ataacgtcat agccctgctc gccccaggta
     9361 gttttgaaaa tcggctgaag ccaaatggta ttaatcccca attcccgcaa ttcggacagt
     9421 ttctgttgaa tatcttgaaa tgtaccttcc ataacaaagg cataaggagt aatctcgtag
     9481 attaccgccg aatctatcca agccgcatga tcgcggcgaa tatcaaatgg ttgaatgtca
     9541 tcaggactga ccgttataaa agtacgagct cgggcggtat ccccgctggc agtaactcct
     9601 gccaaattga aataatattc gccgttggga acttcctccg gcacattaaa aaccaaaagc
     9661 gaatcggtta tttcattcag accaaatgaa gcaggatttc gcccgtcttc gctccagaaa
     9721 aatgtaactg cttccgaatc gggattttcg atgattttac cgtgtaatgt cacttcgcgg
     9781 tcagtgaccg tcgcataggc ttccagaatt ggttttaagg acattttcat ctgtaaacgt
     9841 aatgtgtcag aataaattgg ttcaccttga ttttcaatcg ttgctacaaa ccaattctcg
     9901 ccctcatcta attttacttc gatgtaaaag ctggaatcgg aaggattaat atcgaagatt
     9961 tcacttccat ctttccaata taaaacccca gatgtagcag tcagtgtatc aatttttccc
    10021 caaattactt gttccggttg ccatacctcg atattatgag tttgccactg gagttctact
    10081 gctaacgtcg aagccaagcc aagcccaatc agtgtaatta ttttaactat attccgcatt
    10141 gcaatgcact tttggacttt tttttaccgt actaatattt tccatacgtt ttagctactt
    10201 ccaccatctt aaatatattg gcatctgggg tatcacgtcc acactgatca ccggttgaaa
    10261 gaacaaaacc accgtcatag ccgacatcat caattagccg tttacactta tcttcgacct
    10321 gttgcggtgt cgctcgcatc ataaattcgg ttgtattaat attgccttta atattcaatt
    10381 tccgtccata tcttttctta accatagcaa tatccacatt gccagtcggc ggttcttcca
    10441 ttggttccat cgaagtaacg tccgtttctt ctgctactat ttgcaccaat ggccaggaac
    10501 gaccacaaat atgcaaatgg caaggcatgt tcgccgcttt acaaatgctg gaagcctgct
    10561 gaataaaagg taactcatat ttccggaaaa tatcgggaga acttacgcta agactggcac
    10621 tggaaccacc cagcataatc acatccggtt tggcttcaat catagctcgc agataggcta
    10681 atgcccagtt catccagaat tcatgaactt tttgcatata atcagcttcc atcagcaagt
    10741 cggaaaatac gttctcaaac ccaccatccc ggttgctaaa ccaccaatcc tggaagactg
    10801 gtatcatccc taaatagacg cccaaatcac caatcacctg cttatcacga aattcttttt
    10861 cccattgcca atcgcccttt cccattacca gttgaaattt ctcaaaatct tcttgcaagt
    10921 ttttaatcgg tttgacactc cgccaagggg attcatcggc aaaaaatatt tcttcttcgg
    10981 ttacatcacc tttagccgta tgccaggtac gtcggactag ttttccagct ggtatatcta
    11041 ccgtctccga ttcaaaatcc agcgaacttg atactttaac ctctttcaag ccgccataca
    11101 tataaaaacc atccatatcg aaatatttta cagctttgac ataaacttca ccgtgggtgg
    11161 ctgaagtcca accataatgt tcacggccat ccaaatggat ttcataaaag gggcgcccat
    11221 ccagtcggtc cggcaccatt actgatatat ccggactaac cggaacgtgg tctgctactc
    11281 caccttttaa agcaatcaat aaacgttctt tcgatgtcat tattttcgtc ctatttttta
    11341 atattatcag gcaaacgatt ccagctcctc aataattcct tcaagcgata aaacgactgc
    11401 ttcggttcgc aactttcggt caccagcccg ccattgacaa taaacgggcg aaaattggaa
    11461 aaatcatacc agttaatggc tttaatattg agtttgctgt aatagagggt ataaacttgt
    11521 tccaaccaat cagcttgtaa ttcttcgtcc caggcgcggt gccaatcata tggtctccca
    11581 gagatgccca tactgcggtc cacaacggtt tcaaaagtcg gacctgaggt tgtaccaatt
    11641 tcagtgatat atatcggttt cccaaatttc tcaaaccttt ccagcatccg cattatgtcc
    11701 gataaatcgc gattcccgaa gtagatttga attcccagtg tatcaaattc cacgcctgca
    11761 tcgagtaaat cggtgatgaa ttgatgaggg gtgcgcaaag gccgggttgc ttccatccgc
    11821 gccattctac ctctggcaac gtactcaccc cagggtgcac aattattgat caggcgatct
    11881 actttaggat taatctcatg ggttttatca catacttgtc gcacgatttc ggtgatttga
    11941 tcgggagtat gattgaaaat attcgcccaa tcatgatatt cgtttatgac ttcccaatgc
    12001 aaaacctgat cgccataatg accgacaacg tcgctaatat gtttatctac ataacttctc
    12061 aattgatcat aatttttact tttcaaccat tccggagtta cagtaggatg aaaccaaaag
    12121 agtggacggc cttctatagt aatatttttc tcggttagcc agcgaacgat gttatccttg
    12181 atcccccagt tataaactcc ttccctcggt tcaaataatt cataccaggt gtcccagata
    12241 taatgagtta ctgtagcaaa attgaataat tcgacaaaac gcttggtgaa ttcttcattt
    12301 ttcgcccaaa catactggcg ggattcacag ccaaagtaaa cttcatcctg gcgattattt
    12361 ttagaaatca tttggcgggc atgttccaat tcgatattct ctcccgtcca taaagcatag
    12421 taaagagctt tatccgctat agaggcacac ttttcacttt cggcggtata tttagcggaa
    12481 tcttccagat attcctctgc taaaccagac aaacgaatta cttccggtga aaatttagta
    12541 ccctgagttt gatagcgtcg cataacttgg cgatttcggg ctattcggct tctggcaaat
    12601 tcgtgattca agataagttc atttcgtccc gtcatttcct ttaaagaata aaattctccg
    12661 tcattatcgg cacttaaaaa acagtttcca aagccttcga taaaccactg gcaattcaca
    12721 ctgaagcgtc gggttcccaa atcctcaggt acgacaattc catctccgtt taccacgata
    12781 ccagattgaa aagggtcgcc attggtgtca gccgcataaa caaaattaag cggtggcatc
    12841 cagggatgag gaaaaggttt aaataacaat ggcttagtgg ttttggcaag aatattcgat
    12901 ttttccagaa aagacgggaa catcgcggct ccaactatac ccattgtact tttccgaatg
    12961 aactcgcgtc gtgataaagt tttcttcaag gatggatcat tattcattta ttgtaaaatc
    13021 ctccatttct cttgtaattt tttcaagcga tcgacggccg ctttccgttc acctttcggt
    13081 gaacgcaata agccaccgtt tttcaagaag gaaaatccat caatcaaatc ataccagttt
    13141 gccgcctgaa tcattggctg actgtaagca taggtaaaaa tagcttccag ccagtcggct
    13201 tgaagttctt cgtcccagtg acgatgccat tcatagggta aagtagaata atcaccaggc
    13261 tcttcatcca gaaactcttg cgtgattcct ctggacggtg ccccgacttc cgccaaatga
    13321 atcgctttac cgagcttctc atagcgttcg atgacgccga tataatcggc taatgtcttt
    13381 gaggtaaaat agacctgcac accgattgca tcaaaatcgg taccagcttc gatgatttgc
    13441 tcggtaaatt ggaatggcgt tcgctgagga tatcgagccg gaagctcatg ccacaagcct
    13501 ttctggacat attcaccaaa gggactacaa ttattgatta atattttcaa attgggattg
    13561 acatcgcgaa caatatcaca ggcaaactta gtcaattcaa tcgtctgttc atggttcaaa
    13621 aatagttcat tcgcccagtc atgtaattca ttgaccacct cccatacttt tatcctattt
    13681 ccatagtggc caacaactgt ccgaatatgc ttttccagat aattcagcaa ttgaggaaaa
    13741 gtcttatttt tcaaccaatc cggtgttacc catttatgtg tccaaaataa cggacgacct
    13801 gcaacctgaa tcttgtactt ttctaaatcg gctaagagac ggtcacgttc ggcaaattgc
    13861 ttctgccctt cgactggctc gaaatcaacg aaatcaccaa tcaggtaatg agtaattgtc
    13921 gcataattaa atacttcggt aaatcgttcc agaaataatt cactatccat ctgaaaataa
    13981 ccacgcccat cgcaaccaaa taggaaatcg agccgggcac tatttttagc aatattccag
    14041 cgcgctttct ccaattccaa catttcactt gcccaaaggg cataaaggag agcttgttgg
    14101 gaaaatcgag cgcaggtctc atcatcctgg acatttttga tcgcatcatc taaataattt
    14161 tccgccagat taatcatagc agacaattcg cgagatgcca accaaccttc acggttaaaa
    14221 tttgcttgcc gacgccgatt acgatagatc cgggtttttg ctaattcaaa atttaaatta
    14281 agttggagcg aaccgaactg gggcaaagaa taaaattcac cgccattatc cgcgggcata
    14341 aaaaggtagc cataaccttc aacattccag cgaacattca aggtaaattt tttaattccc
    14401 ttcgtattac tgatagtaac accttgattt ttaacagcaa catcggaatg aaaggtgtca
    14461 ccattcatat ccatcaaata agcgaactcg tcaagatgcg gaccgcgccc tttttgaatg
    14521 aaatatggtt taaatatcaa tgtcttattc ggcatttttc acacaatctt gggcactact
    14581 acaaattttt ccactgttct ttgagcttca acaaacgttg ccaggcggct tttctttcac
    14641 cgtttggact gcgcagaaga ccacccgttt ttatccaagc gtaaggatca acgaaatcgt
    14701 accaattcgc ggcttcgatc cacggtttac tataggctaa tgtatatact ccttctaacc
    14761 aatcagcctg caattcttca tcccaatggc gatgccagac ataaggttcg gtgggcattc
    14821 ctaactgacc ggtattgatg gagtctttag tcggaccgga ggacgcccca atttcagtca
    14881 actgcaccgg ccgtccgaat ttttccagtc gctcaatcag gataattgta tcctgtaggt
    14941 cacgataggg aaaatacatt tgttgaccag taattgtaaa atctactccg gcatctacta
    15001 ggtcctgcat aaattgatga ggcgtgcgct gaggatagat ggcatccagt tcaccccatt
    15061 tttttaactg aacatactca gcaaaaggac agcagttatt aatcagtcga tgaacttttg
    15121 gattagtggc tttggcaact tcacaagcca gtttggtaac attgacaatc tcttcaggtt
    15181 ttaagcgtag ttcgttcgcc cagtcatgag cttcattaac tacttcccag gcatacatac
    15241 cttcaccata atgactgacc acctctttgg tatgactttc gacatatttc agtaactgat
    15301 cataggattt atggcgtaac caatccggag tagtggaatt atagaaccag aaaagtggac
    15361 gcccttctac cgtaatcccg ttttggcgta gctctttaaa aacggcatct cgtaaattga
    15421 aatttttctg cccctctata ggctcaaaat cctgataata accgctgagc agataatgag
    15481 taatcgtggc atagttgaaa agctcggtaa atcgctccat aaatagttcc gggtccatct
    15541 ggtaaaaagc ccgtgaatcg caaccgatga aaaaatgcgg ccggtaacca attttgcgaa
    15601 tttcaaaatc agctttttcc agttccatct tctcgccgac taacatggca taatacaatg
    15661 ctctctgagc atagtcaccg cagagttccg ctgatgcctg tgattttacg gcatcctcat
    15721 agagactgtc ggatagcgcc agaagctctt ttacttctcg tgacggttgc cagccatcac
    15781 gtcggagtaa ttccaaacgg cgccgattgc gcaacacgcg gcttttagct aattcaaaat
    15841 tgagattcaa ctcgcgctgt ttcccttttg ggggcaattc ataaaattcg ccaccgttat
    15901 cagcagtgat gtagatgtag ccgaaacctt ccacattcca gcgaacatca acgccgaatt
    15961 tttgggtacc ctcagtatca gaaattttca ccccttcatt ggatgcggta atgttagaat
    16021 ggaaagcatc ccaatgttca tcactggcat atgcccaatc caataaatga ggtcctcgcc
    16081 ctttttggac aataatcggt ttaaaaatta attctccggc ttccgtatta gttttcgtgc
    16141 gtttgggcgt tagaacaagt ggtgccgata aagctcgtcc accaactatt ccgcccagac
    16201 cggcatacgt agccattttt aaaaactttc gtcgggttat tcttggcata attctatcct
    16261 ttattttgtt cctgatgttt ctaaccactg gcgcaatttt ataagtcgtt cgtaagccac
    16321 tttggggtga cgacctcggc cgcttagtaa accgccattg tcataatagg cgccattatc
    16381 aataaaatca aaccatagca ccgaatcaat ccaagactta ctataaccaa ttaaatataa
    16441 agcctccgcc caatcggctt gcaattcttc gtcccagggg cgtctccatg aataaggttc
    16501 agtcggaaat cctaaactac cgttcttgac cgattcctcc gaaggaccac tggtggcacc
    16561 aatctctgaa atttgaactc tcttcttgaa agattcatag cgctccatat taacaatgta
    16621 atcttgtaaa tctcggtatt gataataata ttgcagtccg ataatgtcaa aatccacatc
    16681 ggcggcgata aggtcacgca caaattgcca gggtgtgcgt tggggataaa gggctttttg
    16741 ccccgaccaa tttcctaagt gaacatattc tgcaaacgga caacaattgt tgatcattag
    16801 cggaatattc ggaaccgtat cgcgcgctac ttcacaggca aatttcgtta attcgacaac
    16861 ttgatctgga tttaattgga cttcattagc ccaatcgtga aattcattca ccacttccca
    16921 gacagccatt cgatccccat aatgaccgag cacttcccgg gtatgattct cgacatactt
    16981 ctttaattgg tcaaaattct tatgcgccaa ccactcaggc gtaacccatt tatgaaacca
    17041 aaacagcaaa cgcccttcta cttttatccc attctgtcgc agaacattgc agagtatatc
    17101 gcgatgggta aaattcagct gcccttcaac cggttcaaaa atgtccattt ccggatcgcc
    17161 attgacgaca tacgtcacgg ctgcaaaatt aaaaagacga gtaaaatatt ccacaaaccg
    17221 atctttgggc atttcatagt atgaccgagc atcacaaccg aaaaggaaat tcgctcggcg
    17281 ccctattcgt ttaatattcc atttggcttt ttccaattcc atcatctcgc taccccataa
    17341 ggcatagtgg agagcttttt gagccagttg agcacagtcc tcgcttaagc gcggttttcg
    17401 gccggcatct tctaacaatc cttccgacaa atctaaatag gcagatgtct cctttgaagg
    17461 ctgccatcct tctttgacaa aattgttatg acgccggcgg tttcttgcca cccgactggc
    17521 ggcgatctcg taattcaaat tatagacggt ttgcttcccg aaaatcggca actcgtaaaa
    17581 ttcaccacaa ttatcagcgg tataatcgtt atacccaaaa ccttccacat tccagcgaac
    17641 attgattcca aagcgcttat gacctttggt attcgaaatt tttattccgt cggcatcaat
    17701 ggtcatattc gatggaaaag tatcgccgtt ttcgtcagtg gcaaatgcta aatcaccgag
    17761 atgaggacca acaccctttt gtacaaaaaa tggtttaaaa agcagttcgc cagaaggact
    17821 gggtgcttgc gactgagaat ttgtgatatt tgccataacc gctatatatc tccccgaatt
    17881 attacttgat ttccatccta ttcaactact tcttatatga tccagaaggg acttctaata
    17941 aaactccagc gcttacgggc gcgccaaaat taggcgaatc atcgttattc caaccaaatt
    18001 tctgcattcg tacgtcacga tcccagccac ttcccttata tttagcggcg tggtaaacga
    18061 tccaatcctc gcgaccatcc gacgacttga caaacgaggc atgtcccgga ccgaatacag
    18121 tttccgtttt cgaaaaaacc ggctcgggct ttttaatcca actcgcgcgc gacaaaatat
    18181 ctccccccgt aaaagttaat tgacccaaac aataatcatc cgtccaactc ccgctagcag
    18241 aataaataat gtgaatttta tcatttttct ttaatatctc tggcccctca ttcaccaagg
    18301 gacgaccgac cttttcccat tcatattcag gttcagaaat cagcactcga tcgccactga
    18361 tcgtccaggg attgctcatc ggtgcaatgt aaagattctg ctgaacattg actttccctt
    18421 cccaacccga ccaaatgaaa tagagatgac ctctatgttc taaaacggtt ccgtcaattg
    18481 cccagcaatc cgttgaatcg gcaattttcc ctaccaaaac ataatggctc agcggatcaa
    18541 cactatcact tttcagaaca tacattcggt gattttcgtt taatccatca tcagccgcga
    18601 cgtagatata ccatttatta tcaaaaaaat gcaattccgg cgcccataat tcttccgacc
    18661 agggattgcc tttcggcggt tgccagacaa gcgttggttg cgctaatcca atttcgtgta
    18721 gattcttgga acaactaatc cagatgcgat ctcccgttga aaaacagtaa taataaaaat
    18781 tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntggaacaac taatccagat
    18901 gcgatctccc gttgaaaaac agtaataata aaaattttgg tatctgatta cccagggatc
    18961 ggcaccatcg gtgacaactg gattagtaaa agtgaccggt ttttctggtt cttcttggca
    19021 atattccaga cagacaataa tcaaaatcag cattgatccg aatatgataa tcttcatcca
    19081 atggccaagg ttcaaaagat attactcctg actatctaca aattttaaat ggacgccttc
    19141 cgtcagacgt ttgtgagtta ctccaaatga gtctgatttt ttgccgtcaa ttaatatttc
    19201 ttccaaacgc gctggattgg ctgatacttt ggatctttct ataataatct gtttcccgga
    19261 ataatattcc ttgtccaatt caatagttac tttctgaaac aacggaattc ccagtcggta
    19321 ctcatccact gccggacaag ccggatagaa tcccagcgcc gagaaaacta accaggctga
    19381 aatcgcacca gcgtcatcat tgcccggtaa accatcaact tccgatacaa actcttctgt
    19441 caaaatactc tgaattagtt cagcagtgcg atattcataa cccgggatat aagaaaacag
    19501 ataaggataa gcaatatcgg gttcattcgt tatggtaaaa tgcccttcgg aaaaacaccg
    19561 ctccaattta attctgaaag gttcataccc accaaattgg tcaataagtc ctgaaacatc
    19621 atgcggcaca aaccaggtat aattccaggc attgccttcc acatagcccg gaccgccgga
    19681 accttcccag taaccagagc cttcggttgc cagcggatca aaaggttcca accactgacc
    19741 atttctcagt ttggggcgca taaatcgggt tttcggatcg aaaaggtttc gataaaatga
    19801 ggctctttta ccgaactcct ctgcaatttg attttgaccc agcatatttg ccatattccc
    19861 gatacaaaaa tcagataggc agtattcaag cgttgtcgaa accggtcccc aaacccacca
    19921 ggcttgactc gtatcttgtt caaatgggat gtaaccatat tttagcagtt cgtggtaacc
    19981 agcccgaatt ggcggggctg attcgccaga atccagtaac gtcggtttga gcattgcctc
    20041 caaagctaaa tcaggatcaa aatcttgtat cccttttaga taactatcgg cgattacgat
    20101 tggtgcagga tcaccgacca tcatataagt ttcattacca gcgagttccc actttggcag
    20161 aaagccagat tccgaataca tatcaatcat cgtccgaata atcgctgatt gctgttcagg
    20221 ataaatcaac gttaaaagag gatgtaaagt gcgataagta tcccagaggg aaaaaaccga
    20281 gtagcgttct cggtcggtat atttaccgac accnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnnnnnnn nnntcatccg aagctcgtga aatatcttca cttacttgat cgtctatcca
    20461 tgtaccgaat ttctgaggag tggcacttac cctcgtaaca aaatatacct gatgctgatt
    20521 atcttcacca cataaaccac ctgaccgatt ataaccctca atttcagtag gcgaaacaaa
    20581 acgaacgcta cctccttgtc gctccactaa actgcgcccg gcatcaatca aaatattaac
    20641 atttccgtct ttgccgatcg taaatctggc aaaactacaa tgcgtcgttg ccgttacttc
    20701 cgccttcaca tccggttcat tcaaattcac cgcatagtag cccggtttag cctgctcatt
    20761 ccggtacgaa cagcggtact gttccggctc agttttaact tcgcctcgcg ttacagtaat
    20821 aatcacactg cccaaatcgg cacatccggt tccactaaga tgaacctgtc caaaaccata
    20881 aaaatagggc tcgccgtgaa tgtagcccga cggcgccttt aaatcagtat gcggactaac
    20941 cgacaccatt ccccagggaa ccagcgcccc cgggaaagtg ttaccgccac cgctcgttcc
    21001 aatgaatgga ttgacaaatt gattaagatc gttaatccca gaagatgaac atccatttat
    21061 aaacagaaaa aaaagtaaaa ataatgatat cttaaagcgc aaaagtaatt ctctaatttt
    21121 caaattttga tgaataatct ctttttccac ataccccagg caacggcata catcatcaac
    21181 acaaaagcaa ttgcaaatag taaagaagca ttgaccggac tggctatcgg taaaaatacg
    21241 ttctcataaa tagcatcatg aagcgttgta aacgtaccat cagctctatt ccaggatatt
    21301 gcccataata gattagccag caattccgat aaagtataaa ctgtaattgc attcatacca
    21361 aaaataacaa acggcttggt gccccaggtc ttacctttca catccacaat ccaataaatt
    21421 gtggcgaagc agttcaaggc ccaaccgttt ataaaaacta cataggagct agtccacagg
    21481 tttttattaa tcggtagcca gatgttcatt atgataccga gcaatagaca taaattgcca
    21541 gcgacaaaca ttccggccgt tttctcttct tgactacgtt cactgcgcaa ccagcgaccg
    21601 gcttgaacac ctaataaagt tgacgcaatc gctggaatgg tgcttaggat gccttccgga
    21661 tcaaagccga ataccggtgc aaaagcccag gtgtgtttgc ccaataaggt tttgtctata
    21721 aaccaagcca ggtttccttc gggttcaagg actcccgctc cgtaaccggg gaccggaatc
    21781 actttgagag caaaccagta tatgcccaac aaaataccaa tccagagcca ctgtccctta
    21841 ggttttacat tcagataaat aagactcgtg aaaaaataac agatgccgat ccgttgcaaa
    21901 actcctaaaa tacgccaggt agcaatcgaa aattgcgatc cgggaataag cccaaacggg
    21961 aagccattaa tgaataatcc taaggcaaat agaatcagca agcgcttcaa agaatgtcgt
    22021 agaatctgct gatgagtgtc accgtgtttc agtcggctct caaaggaaaa taccattgac
    22081 ataccaacca taaatagaaa aaaaggaaag atccagtcgg taaaagtcca tccatgccac
    22141 tcggcgtgta ataattgagg ataaacgtaa tcataagaac cggcattatt gaccagaatc
    22201 atagccgcta tggttgcacc gcgaaagaca tctagggagt aaatacgctt agagtgttcc
    22261 atcttccctc ttaatcacct tctcgtataa atttttcata acgtcttcgg tactcgcttt
    22321 tatactgttc aaccgtctga tcataactca tattttctat gttcttttta aaagaccatt
    22381 ctaaatcaac ttgacgagta tcaactggga gagagaccgc attattttct tggttaattg
    22441 aacgtccgtc tatttcaatc tttcgcactt ccataaacca gggcaaatga agtataatca
    22501 ccgctggtgc ttttttaaaa ttatttttca atactaaagt cgctgaattc tttttaaatg
    22561 ttaaggtgaa attaacttca ccgaattccg tgggtgcttt tttaatgtca atctttgcgt
    22621 ctttttttac ccaattcggc gatattgccg aaagtaaatg tagtgtatca ctttgttcac
    22681 gcaccatcat attgcgcaat agaatacgat attcagccgc aaaccagcca tgcggtgcca
    22741 ggttcatccc aaaatcccgg tccttccatg gtagaatnnn nnnnnnnnnn nnnnnnnnnn
    22801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22861 nnnnnnnnnn nnnnnnnccg ccttctccat ccaatccggg aggaatataa cctccggtct
    22921 gggcggtaat ttctgccagg attcgcatta aagtcgcata ataatcgtca tataactttt
    22981 gtaactgttg agatttttcc ccttctccca aataatcagc tataaaaacg gcgtttttta
    23041 ttccaattaa tgtccagaag ttatggccgg taacgtgtcc ggtaatattt tcgttatcac
    23101 caggagtcgt tactggaatc aagttcaagg gatcagtttt tcgggcgctt tcgagccagg
    23161 caatggcttt taataccgaa ggaaacacct tctcagcaaa gtctcgatca tccgtcatac
    23221 ggacgtgctg accatacgcc cagagtgtct gcccaaatcc gtcgtactgt ccgccttgcg
    23281 aaacaaaatt accatcctct ctttgccagc gcgggaaaaa atctaatata cgattggctt
    23341 cgttattata accggtaagg tcgtacattc gagtaaaata cgccgcatcc cgtaaccaga
    23401 aattatcata ttgaaattca ttaacttttt gaacatacca gccgtcaatt ttatcacgtg
    23461 caattaagct attgattagg ttggcattaa aagtgtcggt tacctttttc tccggtaaga
    23521 caattgtcat tccctcttgt agaacatttt cccagaattg aatcgtctta ttaagataat
    23581 gatcaaattg tgcctgccga atccattttg aatactcatc caccgtatca accggcatat
    23641 agggaaattt gaaatcaaga acgacttcct cgtttgggag catcgtcaaa ggatactgta
    23701 caatccccac cggtgttgtt gggagaacat aaagggtcat ctcaccttca gccggcggtt
    23761 cattatagcc cattttccag gtcaaaaatt tgcgaatttt ctcacgtggc ggaaatgagt
    23821 agagtaattt atcattatat aaaaccgcat cgtcttgatg taaacaaagc cagtcctgac
    23881 ggaattccag ccccgcttgt tcatattcac cgatttgttc cggtaccgcc ggtcttataa
    23941 aacgattatc accaatcccc caatcggtat ttgcctcatt ctggtaacga atcccagccg
    24001 caaaccaggt agtcctttgc tgagaatttc gattaataat ttttacccga acaaaattga
    24061 ccagcggact ttctggctta ccatccaagg ttgcagcaaa aattgtccat cggtaatcga
    24121 aaccgtcacg atttagtgca tattgaatga ccggtagata accatttaac aaagttttga
    24181 cccgtttttc agtcggtatt ggcggattgc cggtgaaaaa catcatttca ccagagccgg
    24241 tatataaata tccttcagga gtaatcaacg tcccctcgcg accatccata actccgatct
    24301 gatcagtcgg tttactaaaa taacaaaaag gttcgtcatt ccgatcaatc gatgggttta
    24361 acatctgggc attcaactca tttccaaaaa gtattataat caaccagaga tataaccaat
    24421 ttttcatgct ttatcctatt gcggtagtgc ccattctacc agctttacgt tcattacgat
    24481 ttcatcgagt actttactat cctgaattat ttgccacttc aacggctgaa atccgtccgt
    24541 ctctttattc ggagcaaaat agaaacattg taccggtaaa ctttctccgg caggaattaa
    24601 atattcaccg cttccactga cttcctccca accagtcagc aaaatcggtt tgacgctcag
    24661 tcttatcgtt tttttatcac cgttcgttat caggagtggt acgtggaaat aactccccac
    24721 cgaaatggca atttccggcg agactattgt cgccaagtgc tgaagatcat gcacctgcca
    24781 gaaatggcgg tagtaatcga aaataccacc gaattgtagt ttaacacctc tcgctactgg
    24841 cgaaatatag ccagtgtttt tatgaaaatc tgctggtttt tcgctgacac cttcaaaaac
    24901 atcaccggtt ggctcacatt ttacaacgga tttaccgtat atcagatgga atttttccag
    24961 ccactcgatc attggttgcc aatttccaga ttgctccgct tgttcggcaa tttgagctac
    25021 atccgcctgg gtttggtgga atttatgcag ttgcgcggct aattcgatat aaggttttcc
    25081 ctgcgaaggt gatttggcac taatatcaaa ttgtggtccg ggagcattta acgcttcatc
    25141 tcgatccgag aaataataaa tcttcttcac ctgccaaggc tgtagtcctt cgtaataatt
    25201 attaatatcc attatttcac gcggaaatgc caattgcgcc ggaaaaatag tcggatctcc
    25261 cgccatatca aaagcttccg ttgcgaaaac accagccgcc tgatgatcac catgattttc
    25321 ccccgctaca aaatgaggca accaggttac aatgatttcc ggtcgggtca atcgtacata
    25381 gcgaatcaac ttctccaaag ccgctccatg aggaagattc tgcagagaat gcaaaacatc
    25441 ttgcccgggt gtgtctatgc catcgagaaa ccaaacatta taaatgccaa aggcacttaa
    25501 tgcgcgccgc gcttctattt cccgaatagc ccccattgag tgatattgct ctttaccaat
    25561 cgaattaccg ccaccctggc cgcgattagt ataaatcacc ccaatttttt taccgtcgtc
    25621 aaaaatccat tttgccagct gaccaccaat tgcagtttca tcatcgggat gggcaacgac
    25681 taaaagaata tctactttgg aacgtggatc gggtgtaatt gcctgagctt ggagaattcc
    25741 gattccccta attgtcataa ccacaaaata aactatcagc gattttttta tcatttgtcc
    25801 tgtcataata ttactctgtt cattatcttt ccaccgattc aaccacaaaa atattttggc
    25861 ggcgtggttc tgcgacataa aatacccacc aaattaacga aagaaatcct cccaagccag
    25921 caattgcaaa gattacatca taaccatata gatccaccaa ataacctccc aacaaaccca
    25981 ataaaataaa cggactggta atcatattag aaattgatat ataagtcggg cgctcctttt
    26041 cgttgcatat ctctgccaga attggcaaac gagaaacttg aatcatactc gtatttaaag
    26101 cagataacac gaacacaaaa taatataaag acaaggagcg cagtgttatt gccagaacgc
    26161 agctgactaa aactgaaaat gccgctatta tcatattgac tcggtgacca aaccgatcac
    26221 cgatggaacc ggagcagaga ttcaagatca ccatactaag catatatatc attgtaaaac
    26281 tgccacttga tgcggaatct aagccgaaac gttgcatcgc attcacgata aaaaacggct
    26341 gagcaattag tccaatagtc atcagactat cagcaatcaa gaaattcaga aagttatgat
    26401 tactttttat caagttgggc aattgtctga aatattgaaa atgattcaat gactcagtgc
    26461 tggcgttcgc ctcctgttcc tttagaaaaa ttaaggcaac gtaagacacg gtcatcatta
    26521 caaacgctaa tagaaataaa tagccaaaat tagcgggaaa cggttgggcg ctaaggataa
    26581 ttttaaccaa acagccgccc gctattccta aaattgcacc agtgatagtt cgcaaggcga
    26641 ataaccgccc ccgcaaatta accggagtaa tctttgaaat taaatcaaac caaccggggt
    26701 agtttactgc gcctgcaatt gccgccaatc caaacgcaat aaaaaaaaag actaatccca
    26761 tgttggacga gacatttttt ataataaaaa aacagagcaa tgccattaat aaccagggta
    26821 atcgttgcag agccgcagtt tttaagaaaa atggcttttt ataaggtaat tggcgggtat
    26881 aattggcaat aaaaatctga ggaaaattaa ctccgataat ccaaattacc tgaaccaatg
    26941 ccacggtaat attattaccg cctacctgtc ggacgaacac cggtagtacg gtcataaccg
    27001 gaacaaagct cattccgaag gcgaaaaagg ttccatccat ccaattcaat aggaaatttc
    27061 gacggacttc cggatttata agcaatggtt ctttaaaata tttcgccaaa gtaaaccttt
    27121 acttctttcg gatttaaggt aatattaaat gatgagtcgt tgtcttgata tacgacattt
    27181 aaggtttcgg ctgaaaattg ttcggctaag tgttttggta tcaatccaca tatatgaatt
    27241 tcggtattaa ttgccgtttc cgtgagattc cttaaaatca gcatcatttg tcccgattca
    27301 tttttcagca aatcaacccg aatctgtcca agggaatcac agtaattagt agatatatta
    27361 gcatattttt taatcagtcc cgccaaaaaa tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27481 nnnnnnnnnn nncgagcttt gaagattttg ggcagtccgt atccgggtac gattttttca
    27541 tgatgtccat tctctcgatt ccaagcggca aaataacttt ccccgatcaa cagcccacct
    27601 tttttcaccc aatcttcgag caaatgacaa atcctctggt ctattaaata aggaaacgga
    27661 aaaatgagca ctttgtaacg acttaaaagt ttttcatcaa tctcaatcgg atggacgaaa
    27721 tcaatgttat aattatgttc atataaagcc ttatgggccc ccagcaaaga attggtagca
    27781 ttcttttcat taccagtagc cgcccagata aaaatttggt tttcaggatt ataactaatc
    27841 gctatctcag ccggttgtgg ctgtgcctcc agtaagaaat cagcattctt ttgtaatact
    27901 tgaccgactc gggcaaagtc ctgtaaccat gaagttttct ctccatccag agttgttaaa
    27961 ccccaggttg gtgcttctcg tccaagaatc tcaggacgat attgccagaa aataaaacct
    28021 ttcaagttcc cggcgatccc actgaagata aaacgcttca catcattaat atcaatagac
    28081 ctcggtaaat caagcgtata gccgtttagc attaacatct cagccgagat aactggtttg
    28141 cctttggcac aactgcgtaa aatatcaatc atcgccggat catccatctg ggtaaatccg
    28201 tgtaaatcgc caaagcgtcc cacattccag ggatcactcg ccgtagaggt tacgggaaag
    28261 ccctgaataa aaacatgatg acacatcaat gggtgcctac cctgatcaat agctttggca
    28321 acttcaaaac gtttcctgac attttcgccc agtgtagcca caaaaaacat ccgccaatca
    28381 ataatatcgt tgaaagtgtt gcgtgttaac ggtaattcta tttcttcaaa actagtataa
    28441 tttcgattcc aggaaatatt taaccgttcg atcgtttgat attttagttc taaccagttt
    28501 ctaaacttta cccgacaatt atcacagtag cagagcatat cgcccatttt actcgcatca
    28561 tccgcatatt cacgcatttc cgccatactg ctagtcaatt ccggttcact gcctacattc
    28621 caaattgcta atgccgggtg attcgtgtag cgtttgactg tttctgcgag aaattggaaa
    28681 aagtgctgca ttacgccatc atgattgaaa cagtaaccta atccaccaat ctgccgatgg
    28741 ggttgagttt ggggaccaat tttccgtccg gacaacgtta ccatagaagc gtcgggaaat
    28801 ttgtgataaa tccaagctgg cgccacgtca aaaatcgtgt tcagcataac ctttagtcta
    28861 aagcgctcag ctaaatccat caattcgtct atatcatcga aacaaaaccg atcttcgctc
    28921 ggctgattcc agcgccattg aacccagaat ttaactgtat tgaaaccatt atctgccata
    28981 ttcttaaggt cattttccca ttcattacga gatggcgatg gcgcccgata atactgagag
    29041 ccgaagggta tataagattc ttttaaaata ttgcgcatca caaatgctct ttattaattg
    29101 tgctaaaatg aattaaataa catactgttt cagatacttt agacctaatt ttaatcccgt
    29161 ttcgactttt tccaccgaac cttcccatac cggatcttcg tgttcaatcg aaagcacata
    29221 atcatagcca aattcctgaa gcgtccgaat gaaaagtccc caatcaattt tacccgagcc
    29281 gggcatccga taacgccacc aaccggattt ccagggaatt ggatcaattt gccggctaaa
    29341 gattccgtat ttgtattgac cttccggtaa aatttcggca tcctttgcgt gcgtgtggaa
    29401 aatccggggc gcaaaatctt taatcacccg gatataatca atccccaacc aataaagatg
    29461 cgatgggtcg aggttcaagc caaaattctc agaggggact tcattgaaca acgcttccag
    29521 aagttctggt gaataagcgt aattcccggg caacccaaat tttacccaat tctccattgg
    29581 acagttttca atcatcaatt tgacattgcg atcttcagca tattttacta attcacggaa
    29641 aacgcaacca atttcttgaa tgttctcctt cggtgtttta tcagggcgag cgccaacaaa
    29701 agttcctaca agctcaacac ccaacaaaga tgcggtattt atgacttttt tcaggtgatt
    29761 gtgataatat tcccgttgtt ttaaatcggg atgcagatta ttatcgtaat aggcaaaagc
    29821 tgaaatttct atctgatatt tgttaaataa ctcttttact cgctcggcat cttgtttgga
    29881 gaaattagcc gcatcaattt gacgcgcctg ataatcccga tcagaagcca ctggccaagc
    29941 cgccaattcc aacgcttgga agccctgttc agccgtccac ttaacgatct cttccaatcc
    30001 cagttggggt aaacaagccg ttaaaaaacc aagtttcatc ttttatctcc tcattgttgc
    30061 attaactagt ttatacctgt atccaacgtt tctcttggtg acttttgaga actgcttcga
    30121 ctatgcgatt ttcttgcaat ccagcttcaa atgtagggaa attttgctcg tggattgcca
    30181 acttaccagt tcgtaatgcg gtgtaaaaat tcaaaaacaa atttttgaaa gtatcaggat
    30241 aaccttcggg atgaccgccg ggataatgag cgaacgaacg actatctgct tctaacagcg
    30301 ccgggtcttt tactaatatc tgattggctt tatcgcggta acctaaccag agttgattag
    30361 gttcttcttg cttccaagca agagacattt gactcccatc aatagcgata tcaaagccat
    30421 ttttgtaacc agcactcact tgagaaacat tgaaaacacc atgcccacct ttttccagat
    30481 ggaacaacac tgcaccagca tcctccgtct taaccgtaat gcattgagtc gaatccggag
    30541 tcacctcctt gcccttgaaa gttgctacat cggattgagg tttcctacgc gatttatgaa
    30601 tgattactag atcggcaaac acttcaatga ttctctgacc agtaatgaat tgaaccaaat
    30661 cacaccaatg cgacccaata tcagccatag cgcgggaatt gccacccatt tccgggtcaa
    30721 cgcgccaatt ataatcttca ggataaatta accaatcttg caggtaatgc ccgtgtgcca
    30781 gaaaaatttc tcctaatttt ccgctaacaa ttaattggcg agcatgctga atgatgggat
    30841 aattgcgata attaaaattt accgcggtaa ggatcttttt ttcacgagct aatttgacca
    30901 gttcttcaga ctcagctaca ctattggtca atggtttttc actgataatg gctttgcccg
    30961 ctaaaatcgc ggctttatta atttcaaaat gcagataatt aggggtgcaa ttatgaagaa
    31021 cgtcaatttc tggattattt accagttctc gccagttttc atatacctgc gagatgccaa
    31081 acttatcagc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ataacgttta
    31201 attgagaata tgcagattcc tgaagtttaa atgtcggatt agatggatgg cgagttaaga
    31261 gatgcgccac atatttggaa taattcccaa ataaataagt tgaaaccggg tgtactttcc
    31321 gccacggttc ttgaccttct ataccgtgga ctcccgaaag gctatcaatt ccatgaatct
    31381 gagctaccgg caaacaacct gcgacttgtt cgtgtaatcc ttcgccggca aaaagaattt
    31441 caggaaacgc tttttgtagc ctttcaatat gggttcgcat tcccttaata aaatccggtc
    31501 cgcgctttac gttgaaggct aatagtgtct gatccagaaa aacggcatca atttggaact
    31561 ctttaatcag acgacctaaa gtttcaatca tcaggtcact ccaagctcgt gctcccggat
    31621 ttatatacgc aaaataaggc tctgtaagcc aatcaccatc tatatccata gcccagcctt
    31681 gtaagcgacc gaaagcatca actacctgat attttttaaa ttccggatac ttcgggtggg
    31741 tataggtcat tcctagtcca ttggtgtgaa tcatcacttt aaaaccaagt aaatgagctg
    31801 tatccaccag ttctttaaat ttttcctcac cacctaaaag atcactggga ttataatcag
    31861 gaatgtggga atcaatgcca tgctcggcaa atcccggcaa atagagtaat gtccgccgtg
    31921 gatcgtgaag gtttttaaat tcgttcaacc gctcaatcat ctgttcaaaa gtatgatgcg
    31981 gtttttgaga ttcacgacgc gcaccccaaa tttccaaaat aaaattgata ttctttgccc
    32041 aattgggaaa attttgtttt tgaggaagtg gttttatctt gaaggctttc tccatccaat
    32101 tacggtattc agccaccggt ttctgccagt cacctgtgaa ttcctcgata aaccattccg
    32161 tttcaaagga agttagcctc tttttggcag gcggttcaaa tccataacta atcgtaaaac
    32221 tatcattttc acgccgaacc ctagctagtt tcagattgga agaagaatca tgtgaataaa
    32281 ttataagtcc tcgatttccc tttattccga tgacaaactg agcatttagc caaaaaggat
    32341 atttatagga caaggtcgtt tgatcgggca tttgctccgt tatcatttga ccgcctaatg
    32401 ccggtaatat tacttctgcc aggtttaaat tcgtcagact ccactctacc agccagatcg
    32461 gttccggagc cgaatacttc gcttgaaatc gcaaaccgga ttgagtaaaa gtgcagtgca
    32521 cctgacaggt aaattgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttg
    32641 aaatcgcaaa ccggattgag taaaagtgca gtgcacctga caggtaaatt gaccggaatt
    32701 atcctgtaga acaatgtcat aaccatcgac tgacgattta tattcgattt tttgggtcaa
    32761 tagagctggc tcggaaacgg ctcgataggt tcgaatcaaa aatagctggt tactatcatc
    32821 accaattcga cttgatctac tattttggca agaattgctc atataagtat ctcgatgtca
    32881 tttattgatt ttttttcaat atctttctgg gaagtctgat agcgaaaaat cacgaaataa
    32941 gtcgcttcgg atttttctaa aggaatgcat gcaccatgcc aaacgccggg attaatcacg
    33001 accgcttcac cgacatttac cctaaatatt ttcaaatttt cctcaggttc tccttcaatt
    33061 aatagcggta aagcaaaagg tgcgtccacc ggtatcaaaa tttcaggagt atgtaaatga
    33121 cactctaaac tatttacccg attacctcga ggaagagtga ccgtgcagag cccgatttcg
    33181 gttttaccat tgattcgata attggcaata ttcgcccaga atttaaaggt atcaccctca
    33241 gcagttggtt tttctcgagg aagcgctacg acctggccga atttggcaaa attaacagga
    33301 ttaacaggta taattttctt tgttagagta ttcataactt tatttttcta attcaaaaat
    33361 ttttatctcg ccaccattaa gtacgatgtt atttagtttg gtttggtcgt cattccgctg
    33421 aaattcatta aaggtggagg cactcccaag ttcattgtat tggacgaccc gcccttttaa
    33481 tttggccgga atcgtaacgg aaatcgtttc cgtgcgtgta tcttgaacca gattggctgg
    33541 ctttaaggga cgcttgctcg gtacgttagt agcaatccag gcaacgaata taatggtacc
    33601 atcttccttt tcaaacgcac gataaaacac atccgaatca ggctgatgct cgacggaaat
    33661 caggctggta acgttgcggt aaggattccc aaaaagcttg cggaaaaata ccagggcttt
    33721 ctcagcaggt ttgggtctat aatcggcata cgcaatacca aggtaacgat tgttataatt
    33781 atcaccgata acttcttcgc cagccggcaa atctttaatt tcataccagg cgattgccga
    33841 taatttttcc tctgccaccg ctaaagtaag acgtttaaat aaatctacgg cttgatactc
    33901 cggtgtatgt tcataatcat aataagcata ataggaatcg gaaacgtaac taccttgcct
    33961 aaatgtgcta tagccaacct ctgccatcca gagtgattga tcatctccat attttttaat
    34021 tacttctgcc atcgcattaa tattttcagt tattttttct acggggcgct cactccaggt
    34081 ttcataataa ttgtgcatat tgacaatatc tatataaggg ctgagaccat aatcacgaaa
    34141 aagccgctcc agaaaatcgg catgataagc cattccacct aaaacgactt tggcttccgg
    34201 atctgctcgt cggacagcct gcgcccctat cttaataaat tcagcgaaag cagcgacgtc
    34261 tcttgtatcc caataaatat ccagatccgg ctcattccag agttcccagg ttttaatgta
    34321 ttttttatag cgagttacaa gatcatccat aaattcacca aattcttcga aatccagcgg
    34381 ctggtcattc cagaaccaga gtgtatcgcc tcttgaattc cagagcggag tatagcagat
    34441 atagggaatt aaagttatac cgtattgatt cacggcagtt ttaacataat catcccagaa
    34501 taaccagtcg tattgatctt tctgcgcctc aatagcatcc cagccaaaag aaatccttaa
    34561 aagatcaacc ccagttcttt ttaataattc catatcatta tagataattt ccatcgtcgt
    34621 ggtctcttcg ggataatctt cacacaaacc aactggaatg tgaacaaatt cagcgtcttt
    34681 tgtcttcatt aagcgctgat aatttggtga attgggtctg ctacaatcaa caataataag
    34741 cataattagc gctaagaatg attggagaca gtaatatttt ttcatacttt ttacctttct
    34801 taagacgtta ggtttagtca atttatacct tattaatacc ttaaaacatt ataataatcc
    34861 atacttttta gtccattcat ttattaaaaa tcaacccctg aaatcagaat aatgttggcg
    34921 taaggtgaag tttcaccgct acgaacaaaa acagcactgt ccgattcgcg ataaatattt
    34981 ttaaattctt catgggatac atactctatt ggaacacctt ttaataagtc tttcacttct
    35041 tttaaaatac cattattaat tgattcaatt tccttagcca ggattgcctt ttccacaatc
    35101 aattcctcaa gtactgcttt taaagtatca atgaagcgag gtacattagg aaccaaagct
    35161 aaatctacaa tttcggcctc gcgtggtaag ggaagtcctg catcacaaat aactaattga
    35221 tccgtgtgtc ccaatgatgc gattaccatt gagagatgag agttcaagat tccgtgtttc
    35281 ttcatcgatt ttatatcctt tctattgatt taaataattt gaaatatcag cgagggttgg
    35341 cattgaggtc tgagcacctt gcttcgtcac cgaccaggcg gcaactttat tggcgaaagg
    35401 tatcgcttca tttaccggtt ttccagatgc cagcgccgcg gcaaatgctc cattaaaagc
    35461 atcaccggcg gctgttgtat caacagcttt caccggtgtc gcaggaaatt cttgccagct
    35521 atcagaatta accaatatcg caccgttttc gcctctcgtg gtaataacat tgccgactcc
    35581 cgtattaatt aacttccgag aggatgaaat aacacttgcg cgatccgtaa ctgactgacc
    35641 agttaaaaat gatagttcaa tctcattggg gatcagaaaa tcaataccat tcaatagact
    35701 ggcaggcaac ttacaagccg gggctggatt aagaataaac accgcaccat ttttcttggc
    35761 taatatagcc gccgcttcaa cagcactcag aggaatttcc agctgacata atacaatttt
    35821 tgccctttca aatagctttt cctttgatcg tatgtcgctt tccgtcaacc gcatattact
    35881 tcccgaaacg acaactattt cattttgtcc gtcggcatca acgtatatta atgccacacc
    35941 ggttggcgtt tctgagtcta taaatatctc ctccatatga acgccattgg attccatatt
    36001 atttaccaaa cgatcgcgaa aaatatcatc tcccatttta ccaatgaaaa tcgtatctgc
    36061 tccaagtttg gcacaggcga cagcctggtt tgccccctta ccacccggat aaattccaaa
    36121 cttttttccg aaaacggtct cgccaggttg aggatatcta tcaacttggg caaccagatc
    36181 catattggca ctacctacaa taataaccga attttttgtc attaatatct ctttaatcta
    36241 ttttaatttt aagtaattga taatttgtaa ttactggcgg ctttaaaagt cctgatgatt
    36301 taggaactgc ttccagagta ttgattagcg tattggtaat agaaaatcgg aaggtatttt
    36361 ttcccggtct gactaagtcc gtaatatcaa tttgatatgg atgccaggga actattatcg
    36421 gtccgctatc attaatctgt aactctaaga catcttctcc acactcaata tcaagaaaca
    36481 gcttaccttt acaaaagtct gccggaatcg tcagctctgt tgagtaggtt ccagtgcccg
    36541 agaaataggg gtatccctgt tgacaccagt caccaatatt tacctccttt ttcggcaaaa
    36601 caatactata cccatcgtca ttcaatttca aagcaaaatc acccgtgatt tttataaggt
    36661 ctaaaatacc gtcagttcta cgttgtacaa ctaattgaac cgcgattaga ttacgacccg
    36721 ggtggacata agaatggata ttaatttccc gaatttcagc atccaattga ctgcgttggc
    36781 cagtatcctt aacttccgtg ccattaataa ataatcggaa agaacttcca ctaaaaccat
    36841 ctataagaag ttttacacgg ttaggtaatt cctcaatctg aaacggaatt cgataccaga
    36901 gtgtctgcgg atagtggata tcatccggtt cttcgggcaa ttgcatttcc caggcgccga
    36961 tagtaactgg tagccagtga tcatcatcat aatcgagtgc ggcgtagtta tcatttgaga
    37021 attcatttga aaccagcatt ttccatttag caagacataa tgtattatca ttttctattt
    37081 taaaattcat aacctccggt aatattagag gcggtggatt cttcggcacc aattggacaa
    37141 tatccttttg tttaccacct gtttttaaag tcaatttggc aagatctcca ttttttatat
    37201 agccggttat aaaattggct tcggtttttt ctaataccag gttggttgca ctaatataag
    37261 aatcaggtaa gtcattatgt aaaataacca gaaatgtttg gctggcgccg aaggttaagc
    37321 gaaaacaaag tcggttattg attatatcaa aaaccgagag ttcctttact gcaccggtcg
    37381 ccggatccca tagctcaggg cgtccaaccg tctccaatga aatgtccacc tcgccaatat
    37441 cttgctgaag agtattaaca aaatagaaca agtgacggcc atcttttacc ctgtgcaggt
    37501 aaaaaacttc ggaatgtccg atctggacat cggcggaaat acacgactca atggcttctt
    37561 tgatatcctg tctacgccga tcttttgcca gcccgcctcc ttgtattaag cacacccccg
    37621 gctggagcat tttcagctgg ttaacggcgt ttttcccatt ttggaaatct gccaaaatac
    37681 aatttggctt gacgccaaat agttcatcta cagcgcgaga ttttgacggt ttgccatcaa
    37741 taaattgttc aaccggtaat aaaatatcgg caattaactt accaccgtta tttacgaaag
    37801 cttttagttt atccagagtt gcttttttaa tatgagtcac cgggggtaaa atgatcagtt
    37861 cgtaagattc atcggcgatc tcaagtcggt taccatttaa ctgagcatta attagaatgt
    37921 cctcatcaac ataatcaaaa tcataatgaa gatgtaacag ggcatccgtc agataattaa
    37981 aatccatttc aatcaatcgg gaaatatcgt ctggagcctg cggtacatag tttgcccaaa
    38041 tactcgtaat cgggtagaga atgagaattt ttgcaatatg ttttccaccg gttagagcat
    38101 gtcctaaccg cgccatatag cgagtaaatt cattgtaata cttccaccag gtatggtgat
    38161 aaaattgcga cggtggccag tcacgcttgc gttcgccttc aatggaataa tggtaaccat
    38221 gattattaaa tagattaact ccgagtgaat attcccagtt ggcaatccac ttcatccgtt
    38281 ccaaagtgca atcccaataa gtacctccca ttgattcgca aagtaatctc tttttcccta
    38341 aatggtgagt aatcgagctt gccatcttaa gttctacatg ttcgccgggc tgttcttccg
    38401 aaccaatttt cggatataga tgatccactc cggttaaatg taaattactc aaatgttgca
    38461 tcacattgcc ttcacagcgg gcatgacctc ttaaccaatt ttcaaataga atgtggcctg
    38521 tgaataaaac gccgttttca gcacaccaat ctcggagctg tttataataa ctatcgtcat
    38581 actgatctga aagcgttttc cagaaatcat aacgaattcg ggctgtatcg cgaccgacat
    38641 tagcatataa agccgctagg tgtggtttta tactatatcc attacgctca cgaaatatct
    38701 tgaacatccg ctggctccag ggaataacat aattatcagt ccctacttga aaatagtgca
    38761 tagccggttc atcggtataa aatcccggaa cgactttccc aaattctgcc cccactgcgg
    38821 tcttatattg ttcatgcgtt aattcaataa aacgtttagt ggcttccgga tttaacgcat
    38881 ctatatagta atctatttcc ttttctaaaa aatacattaa tacccagttg ccagctggcg
    38941 cttcccacgg aataacttta ttaaatgata aattcggagt tacatcaata atatttttga
    39001 tgccggttcg atattcctca gctgaacaga tataagcggc aatcggtttt gaatcgccgg
    39061 tatttatata gcgtgaatcg gtggcttcaa gatagatgaa aagtggacca ttaataaata
    39121 gtgtaaccat ttccaggtac ttttggctta aatatggata ttttttaata acctggcgtt
    39181 cggctgtgcc gcttggccag ttcatctcat cgtaaatcca gatgttcaag ccgatttctc
    39241 gacctttttt aacggcaaat tttacccgct caaaccattt ttcggataaa tagggaattt
    39301 ttaacccgaa gcgtccgtga ataaataaac ccgtaatacc tttggctttg tattctttta
    39361 actgccactc catctccgcc tcgtccattt cgccgttcca aaaccagaat ggcaaaatac
    39421 ccatttccag cgggggattc ttaaaatttt cacagtttaa ttctttgtct ggccacagtt
    39481 catttagcaa cttcatcaag taattcctta acctttgtta attttcaatt tgcaattatt
    39541 agtttttcaa tttgccaaaa tatcaatcag ttattttaac aattggaaaa tcttagtcgc
    39601 cctcgggatt ggctattttt tttatcatag acaagtaaaa tgcggtaaaa aagggataat
    39661 tcatcttcat taaaaccaat tcatcataac ctaaattgag attattaact cccatgccaa
    39721 tatttttaaa accttaacat cactgtaaac tgatgggctg aattaaggta atcataatcc
    39781 acgaaggaat aatccacgac aagattggta aaatccatcg gaatatttaa tgccgctcca
    39841 acactaaaat gcccctcttc acgattggta cggtaaccag tacgcaaagc aatccgattc
    39901 catagccagt actcggcgcc aatatttaac cgttcggcat aatcattaga gtgaatcgcg
    39961 tcaatgttca gagaaagacg ctgtttatta ggatttccgc caagaaacaa atctttgcca
    40021 cctaataaat ctattgacgt ccctatccga aatactaagg ggacctcata ctgttgttct
    40081 tctctctgct gggttttcaa ccaacgggct tgcgaagcaa aattttgcgc cgtcattgca
    40141 agccgaatgc ctttccaccc cgtattataa tacgtaccaa catcaaaagc ccataaactg
    40201 gttgaatagg tataaaggtc ttctcttaaa tatttggtgc gaatacctat agacaatcta
    40261 tcggtaaact tttttgaaaa tgaaagacca actgagagat cgcccccgga gaaatttaat
    40321 ccggtgcgag tatcaagttt accatcggga ctggttacta gggcttccgg aatatcaccg
    40381 taatccatcg cgatataatc aagcgcaata cttcccaatc ggtcaatcgt tagccccact
    40441 gctccggcgg taacatttag atccccaatc cactttgtat agctgaaaga agattctaat
    40501 cgttgcatct gagtaatggc ggcgggattc cagaaaacag catcaatgct atttaaattt
    40561 gcgatacagg cttcccccat tgcagatgag cgggctccta tcccgatttt taaaaactga
    40621 agggaggtgc gaccggcttt attaaaacct tctaccctca gatttgtagc gaaaaaaatc
    40681 attgcagaaa gagttacaat tttaattttt ctattatgta acatcatttt ttattcctcg
    40741 gcatatcaat tttactcttt agcttcattc agcgaataat cattagctta ccaatcgttc
    40801 gtttcccttt tgtcccttcg gcgtgcgatt caatggcata aatataaaga cccggtgcta
    40861 ctctcaaacc agaatcggta cgttgatccc aggctacact gccaaataaa ttaccgtaag
    40921 tatctggccc atgttcaatc gttttgacta aatcaccaga aacggtgaaa attttaatcg
    40981 tcgctctttc cggcagatta cgaaattcaa ttttataagg cgtcggatca ccaaaactac
    41041 gctcagggtc accttctatg tagggattcg gggcaacgaa tacatcccag ccagcagtaa
    41101 tgtcaactgg tcctggtctg gctcccgtca aaggagtcat attgtaatca tcactgacca
    41161 gttcccctag attatcaacg atagtatgat tggctgaaac ccaggtttta ggatttgaat
    41221 aataagcctg aacataatac cggaaatcaa aaccaaactg caaatcctta tcgagataca
    41281 catatttacc aatttctgca ttccaatagc gattggcttc ttcaatggga atgtcaacaa
    41341 cttcatccca aatgccccgt ttgtcaatac cgcggaatat tcgatagcca ctcagatcag
    41401 ttgaaccatc ataaaatgga ttcccagccc cgtccgttat taccgtttct tcggctagtt
    41461 tatcccattg aatcgcaata atggcagtat ctgaaccttg agaagcatta gttgcgctac
    41521 aattaggggc tggtggtgat tcgggcacat ccgccgctaa attaattcct tcaaccgttg
    41581 ctccaaatcc ccactttaat gctctacgag cggcttcggc attacggcga atatcggcaa
    41641 tcgaactatc gggataatag tgattgggat ccccacgaat cacttcatga taatccaaac
    41701 tcccggctac ttcagcgagt attaaccgca cagaatcacc tggcgcgaga taatatgggc
    41761 caaaagtagc agtataaaaa caatacatat caccattatt ttcttcagga atccgattat
    41821 gagtgataaa atcgaacatt cctttttctc ccagccgttc aatcgaaaag tactcattca
    41881 tatccaaagc tcggccttgg aattgctcac cttcatcgga ataaatattg aaataattag
    41941 gatccgcctc aaaaggatcc gcgtcagaat ccgtcggagg agttctatac agaaacacaa
    42001 atccaaagca ggcgggatct tgcaattcta tattttcata actttgatta tacttcaatc
    42061 gtgtttgcca atccgatctt ttactatagg tatcgtttgg atgcggctct cgccccccat
    42121 taaaatcccg actgtaaaaa aacagtccct tgctatcatc cccatcattc tcgaccgcaa
    42181 aataatcggt atatgttcca ccattttcaa gttgtttcca gcctccgaat ccaccagccg
    42241 gagccgccga actcataaaa tcaggatcgt aatgaaaatt cagatgacct ttagttgaga
    42301 cctggattcc gctatgaaaa accacaaata ccgaatcaaa agtttgttcg taatgtttga
    42361 cttctcccag agctggaata accatctcac cagtattgat aaatacataa tcataaacaa
    42421 taaaatcatc ataatccgga aaactccaag ccatagagcg catcttaacc gaaatgccgg
    42481 ttgctgttgg aaattcgcag taattgattt cctccggcaa taatggatta aactgggacg
    42541 tccctacgaa attattcgtt tttataaagg gtggataatt ccataaaata ccatgacttc
    42601 caccagcaaa ggagctgtga taaaaaagat attcctgata ttgtggatca taatccggag
    42661 ctactaatac atcaccgcct ttaattacta atgccggacc actacgaaaa ttgtgaaaat
    42721 tggcatcagt aacccagccg ttagcaagtt gttcattatt gggaaatgaa tatccagaaa
    42781 agccaggata aaatcccaaa ccggaagtct cttcataatc ccagacgccc aaatctccga
    42841 ttaaacctac attattgagc gtttcccata gtttcccgcg agtatgggtt gacttccgct
    42901 gagcaaaaga ttcactccaa ccagtcaaca gtcctaaaat gataactaag tgaatataat
    42961 aattttttac tttcatagta ccaaccactt ctattattga aaacttaaag ccatgcccac
    43021 ggtgattttc ctgggaaggt ttgtatattt ggcataatct ggaaaagggg caccgttggt
    43081 cattttttcc ggatagacca ttaagttaga attgatataa cgaacttgat cttcggccga
    43141 aatatcacct tccataagat caacattatg ccatttatca ttaaaagcat tctgcatctc
    43201 aatgaaaatt gcaggcctgg tacccttgaa tcctttgaat tcttttctca cccgaatatt
    43261 ggcattctta tttgccgggc gaatgatgtt aaccgctccc tgataaacgt cacttgaaat
    43321 ttgcttatgg ggatacgaaa cataataatc aattgagaac tgtgtatttg ctaaaacacc
    43381 acctaacggt gaaaaattcg acggagccgc cagagtcagg ttagcactga gactgggacg
    43441 catatagtat agttgatcac caatatcaaa atcctggctg gcatgaaccg ttacattggg
    43501 agggtaaata gtaaaagggt caccggattg tcccacgata cctgtttccc aggtataagt
    43561 caaataacct ccccagaaac cacgtaaagg acgtcgtaaa gtaaactcga cgcctttggc
    43621 atcagcataa tcagaattac cggatgtatg atactgttgt tcattatatg ttatgacagt
    43681 aatacttcga acatcgttag tgcgatcctt ataaaaagcg gtaacatccg ccaatactgg
    43741 tccaaaatcc tgctcgatac ctaattcata cgccactgtt ttccgcgggc gcagttcacg
    43801 attccccaga ttacggggtt cgatactgcc aaattcagga tcaacaatat aagttgataa
    43861 aatgctatag gtctgctcac cttcaccata ctcgccgaat gatgcacgtt ggaaaaaatg
    43921 accataatta aaatgtaaga gggatttctc ggttattgga tgtgacaccc caattctcgg
    43981 ggataattgc ccaaatgtat aggttggttt ggttttggga ttcggttcac gtccttccat
    44041 ttctgccgta ataatatcga aaggatcgta tggatcgagg taaataacat cattaggatt
    44101 ataaaaatca tatcgcagtc cgagattcat aattaatcct tcaaattcca gcttatttga
    44161 ccaccagatg tttccctcat aaggatggta gattttatcc tcaacggctt cccaggcgcg
    44221 cgcctcttga tatcggtcaa aagtgtaata agtaaaacca aaaccactcc gcatttgcca
    44281 gtttttattg atctgacgag tcaatttacc cgtaagcgtc aaatcggtat tacgatcctc
    44341 cttacgatac cagtaaccga tatagtgtaa accatagatt tccgcccagg catcgggtat
    44401 cgcaaagcct tgcgaatcgt aagctcgtaa ataatcccga cgtatatcgc tggtggtcca
    44461 gtctgccgta tcgctcggaa acttcgtctg gctctcataa ttgaataccc gcgataaatg
    44521 gagttcgtaa aaagtatttt tatcgagatt gtgaacaaat ttcaaagtct gaccattgcg
    44581 cctatataca gggtaaccag cttcatcata ataagcatac ttattaccat aatcaccttc
    44641 gtattccatt gattccatac cgtgacaagt attatcctga ttataatatc ccgaaaatac
    44701 caataattta tttcctccca aagaatagct gattttataa gtaccatcaa gccaactacc
    44761 catcttgcga taagcattac ccgtaattgg tggtgccgta cgataacgac cggataaaaa
    44821 gaacctaagg tttttaatcg gagtcggtcc accgaaagaa aattcggttt ggtaaccact
    44881 tcgattggtg taatccgtca agggttgatg agtatctata taaaattgat aagcctgctc
    44941 cggtgtccag gaacatgacg gatcgttgcc gtagcgacca ttcggatcaa cccacatatc
    45001 gggattatca atccaccatt gcaaatggcg tgcatgcgta ttctcccaat atcgctctgt
    45061 cgaataatcg taaagtgcag ttccccagtg atataaaccg ggaggaatcc agcgaaattc
    45121 tactgcaccg acataatgat ccggtccctc tttagtaatt atattaaaaa cacccgattg
    45181 agcttcaccg tactcagcat caaaaccacc ggtaataatt tccagttcct caattgccat
    45241 cagattaaca ttaaaataag gctgagaatt caaaggattt cgtgcccgca ttccatcaat
    45301 cataaatgcc acctgatcca acgttcccat tcggactgtc aactcaccgt agggattata
    45361 atcaattcca ggcattgagg taagtatatc ggaaagctga gataccggcc gggattttat
    45421 atcagtttct tcaacaacga ctcgactcga tgttttatct aactcgatca gtggtctttc
    45481 ggctacaact gtcacggctt cgccaaattc caatacggtc tcttctaact caaaatctac
    45541 gcgagtggtg tggttaacac taacgtttac ctcagtttta ataacttgtt tatatccaat
    45601 catcgaagcc tggagactat aaataccggg tgcaacattt atgatataat aatcaccttc
    45661 catatcggta gcggctccca tctctgtccc gactataata atgttcacgc caggtaaggg
    45721 attgcctgtc ttcttatcaa taaccttccc tacaatctta ccatcactcg ccgcatatcc
    45781 caacgaaata agcaacataa agccgaggaa agttcgacta attaatctcc tgatttgcaa
    45841 atatgctttc ttggaaatca ttattttatc tctacgaaag tttgattgtg tcgttttatt
    45901 tattttacta atcattattt tcaatcccat taaggttcaa tgacagcttt aataaatcct
    45961 ttgggctttt cctgcgcgga gcaaaacgcc gcctcaatct catctaattt aaatttatga
    46021 gttatcaatg gtttcatatt tattttctgc tcatttaaga gcttgatccc aatttccgct
    46081 ccgcgtaaaa ttgtcgccat atctctaaaa tgagcattga caatattaaa agccatccaa
    46141 ttccaatatc ccaaatcttt aataattctc tgccctggat gatatccaaa aataactaac
    46201 ttcccttcca tccgtagtaa gtcaacggct aagtcgagac tcgcctgatt accaccgact
    46261 tcaaatacta catctgctcc ccgaccatta gtcatatccc aaataatagc tgggacatct
    46321 tcttcctgcg gattaatgac caaatctgct cctaacattt ttgccttatc gagaatttca
    46381 gcgcgaacat caacgccaat aatccgagaa gcacctttca aagcaatctg ttgcatcaag
    46441 atgagtccca taaatccagt tccgactaaa actaccgtat cgcctaattc aactccggct
    46501 ttgattaccc catttgtcgt gcaagctatc ggttcagcca tagcttcggc aaaactaatc
    46561 tgatcagcaa tggggaaaat ttgattggct cgaactgcaa cttcttcggc gtaaccagcc
    46621 cgatcagtcc agactgcaac tcgatctcct cttttaaatc ctttaacggc acttccacat
    46681 ttcagaattt caccagacac ttcatgaccg atatatcgcg gatattccaa gttatcgaga
    46741 gtttttgccc attgatgaat ttcggaatga caaactccgc aggctcggac gcgaactaag
    46801 cattcatcat cctttatatc cggactttct tttgtttcta ttctaaaatc atgggcgcca
    46861 taaagagccg cgatgcgcat attattcctt tctttacatc aatttttcaa cgaccaaagt
    46921 atagtcagga ggggtggata aaaatttacc acccctcctt tgactttttt tatttcataa
    46981 gtatcatttt acgggtagaa accactttat ctgtaactaa ctggtagaaa taaataccac
    47041 tagataaact agatccatcc cagattacgg aatagacacc cggagaatat gtccggttat
    47101 ctattaaagt cgcaacctct ttccctaata cattataaac cttcaacgta atcggaacac
    47161 gttcattgac ttcgaaccgg atcgtagtag tcggattgaa tggattcggg tagttctgcc
    47221 gcaatgcaaa cctaactggc ttctcgctaa cttccgccac atctattccg tttaatccag
    47281 taccgaaatt cagcagagcc cagtattcag aattttgctg aatataagtg gcacaccaac
    47341 ccagatctcc cggatctgca aacggttcat tcggcacatt cggctgccag gtgtaatatc
    47401 catacgaatc acccgtcgtc tcacgagaat acgtcgaaga aaaactacca ccaacattaa
    47461 atccaaccga agcacttgcc ttaacattgg gattgtaaat cgctaactct acatcccata
    47521 ctccggtctc cttattccac tgcgccgccg atcgtataaa cgtcgaagga tcaaatatta
    47581 ccgccgagtc taattcaggt acatataccg gataaccatc attaaatgtt acccgatctc
    47641 ccattatcat caaatgataa ggaccatccg gtgccgccat cggattccct tcccagttat
    47701 cccaagattc cactcctaaa cggttcgaaa cccctataaa taactggtca ctcgcccaat
    47761 gtccagatgt gtcagtcgga tccaaaaatt gaccacccca gtacaaatcc gtcgtgtcat
    47821 tgacaaactc atcaatatgc ataaatacat atagagtatc atccgtccac aacaaacgcc
    47881 catacaactc atcatactcc ggctcggcta tctcacgacc ataagaactc gcccagatat
    47941 tgtaacccgt ctcagtcact aaattcactt ccgccgcgtt accccaaacc gactcatcca
    48001 ttactccatc aatcactaca tccgcaggtg ataccttcgg aacattcaac tctaaacgca
    48061 caattacgtc gtccggtccc ggtagaaaat tcagcagagc ccagtattca gaattttgct
    48121 gaatataagt ggcacaccaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    48241 nctccatcaa tcactacatc cgcaggtgat accttcggaa cattcaactc taaacgaaca
    48301 atctcttctt ggcttaatgc caccgattgt accgccacta atagcaataa tgctaacaaa
    48361 cacatattaa ccttacgcat acttacctcc atttggttaa taaattggtt aataattcct
    48421 tgtcctgttt tacaaaaata aagtaagcct gacttataaa ttacacctcc tttcatttgt
    48481 ttttttacct ttctcataat tgatactcaa cgtttattga tggaataatt gttttagtat
    48541 catatttacc ataaaaatat tcatcctggc ttttcttgat atctttagca actcccacag
    48601 aataagcaat actgagatct agataaaatt ggttaaataa aaatccaata ccagccgata
    48661 ttctatgttg atttacattc ggcaataaaa gactataact atgatctgaa ttcggggttg
    48721 tctgataatt atatccaacc cggtacttca aattattctc gcctgaatat tccaatccga
    48781 tagcaaatac ataactatta tgatcgtaaa acggcattcc cgaacgccag taaccggtga
    48841 tactatcacg cggagccgca gctattatgg cagaccaggc agtgtcacta taattaaaat
    48901 ccagtttgga ttgagtgctg ccccagaatt cgctggatag atcaatattt agatttagtt
    48961 gatcggtggg tttgtataaa attcctatac caccagtcat cggcatttga aaacgactgt
    49021 gaagtcgcga tttcttcgcc agactatcaa gcgcataaaa gcaatccgtt ttagcattac
    49081 cttcaaggtc aaaatctacg ccgctccgaa tgctcccgcc taccttaatc gtcgttgtta
    49141 taggtaaaat aaatcctagg ctccaaccta tggcattatc ctcttgttct aaattcaaag
    49201 cgtagaccgg attagtcctg ttcgtctgcc actcaggatt cgaaatcgga aactcaaatt
    49261 gttggtaaat ctgataaata ttaatcgcgg cacccaatga cattttcccg acttgtaatc
    49321 cgaaaacctg cgaataggca tccgcaatca gacgtgattt taattgaaag ccatacaaac
    49381 gggttattcc attttcagta aacattaatg ccagtggcca tcgaaccgaa taatcaaggg
    49441 ctcgattata gaccatagcc agcccaaact tttggttagg cacccagtaa atacccgctg
    49501 aaccgctgaa atcattgaat aaataggaac gatggatatc acgacttcct ttttgacatt
    49561 gttgttgccc agctcgatca atgatattca tcgccagacc aaaacccggt gtgcttaaaa
    49621 ttttacccgg attttgaaac gtagttgtta taccatctaa tccggcataa ttataacccc
    49681 ccaaagctag ggattttgca ctggttattg gagcagtact cgcatctccg gcttttgtaa
    49741 aatccgaaag aatcaaaacc attgaaattg tccaaaagat aatttttttt ctcacttaga
    49801 atttcctctt caataaaact ctttcaaatc accaagtatt aaaggttata tatgaatgat
    49861 acttagtgga tcttaagtga tcaaccccat tccaatcctt accagccagc cagaataaat
    49921 aggtcttatt gcgggacaaa ccttttgcag gatattcaac aaataccttc gtcccgggaa
    49981 tcgggcttcc tattaccagc ggctgaccta ttacattgtt ttttccatat accttttgac
    50041 cacctacggt atccaccgac caggcttccc agactatttt tttggggcta tagctctcac
    50101 ctaaacagat tccgaccgcc gctacgagag aatcttcaac gcttgaatca ataatctccc
    50161 aggttatgac cggtgaattg ctggtatccg tttcaataag agtgattttg gcaaatccac
    50221 ccctcaactt aatgcttgcc atatcaacat ttgtataaac atctatccag caactctttt
    50281 gaacatgact gtaactgtct atccataaag tatcctcatt ggcaatcgtc gttaccgtgt
    50341 ccagttctgc cattaagatt actttaccgg cattttctac tacggcatcc caagcggctt
    50401 cctttacaat ccaaaatgta taggtgctgt cttcaattaa tgcttcaaaa tgtccaccat
    50461 attcatttgt tatatcagtt accccttcgg gcaattcgcc atatttaacc gggtagtgca
    50521 aattgtcacc atcaaccttt attaaccatt gcaaagtact gtcaagccgt gcttgatcgc
    50581 cgcgatttac tccaaaagca catccatatc cacccaacca agtaaatttg ggcgtaaatg
    50641 tttcctgttc aataataata ttgttcattg gccgcgcgcc ttgatacgga gccagcgtat
    50701 tcttctcccc acagtacctt aaaaggcaaa gtaaaaatag aattaaaatg ctacttgccg
    50761 aaataaagtg gtttggttta aataagtaat tcatagaata aaccttatgc tgtttttact
    50821 aataattatt attagcctct tcttaatcac gatactatcc tttaattcca cttaaagtga
    50881 taccttttat gaaatatttt tgagcgaaga aaaagacgat taaaattggt aaagttgcca
    50941 ttaccgaacc agccattaat aaatcccagt ctgtaatata tcggtcgcgg aatatagcta
    51001 gtcctaactg aaccgtgcgt aaatgatctg aattgaccac gattaaagcc cagataaaat
    51061 cattaaaagt ccccataaat gtaaaaatgg ctaatgttgc cagagccggt ttcgataaag
    51121 gaataattat ctgccacaat actctaaatt ttccacagcc atcaatatag gccgcctctt
    51181 ctaattcctt aggaatcgtc aagaaaaatt gtctcaataa aaatgtgcca aacgccgaag
    51241 ccaagcccgg aacaatcata gcttgatagc tgtcaaccca tccgagccag tataaaacca
    51301 taaaactagg aatcatcgtt acctggtaag gaatcatcat agttcctaaa aaaatataga
    51361 aaagaacatc ccgtcctttg aattgtaacc gagcaaaagc ataagccgcc atagaacaag
    51421 taattagctg ccctaatgtc acagtacaag ctactatgag actatttaaa taatatcgcc
    51481 caaagggagc cgcctgaaaa gcccgagcat aattctcaaa atggaacttt gaaggtagcc
    51541 attttggtgg atacacaaat atatccccaa tatcttttac cgaagttagg agcatccaga
    51601 taaaaggtgc aattgtcgct aaagctaaag cgtatataat aacatgaagg ataatatctt
    51661 ttactatatg actacttttc cgaaaatcta tatccattat accgcctcat aaccggcacg
    51721 ggtttgcata aactttactt gtaataccgt tagcaccgct aatattgcaa agaaaacata
    51781 agccgccgca gaggcatacc ccatctcgaa aaatttgaag gcattctcat aaatataaaa
    51841 taccatcacc ttcgttgcgc ccagaggtcc ccctgaagtc aaaacataga caatatcaaa
    51901 gacctgaaac gagctgatca ttgacattat cataatgaaa aatgtggtag gacttaacat
    51961 cggcattgta atattccaaa atttcgacca ccatccggcg ccatcaattt cggcggcttc
    52021 atagtaatga tcgggaatcc cttgtaatcc ggcggaaaat aaaaccatat ttgtcccaaa
    52081 tgttttccac actcccatga taataagagc aaccatagcg gctttcggtt cgttgagcca
    52141 atttaccgag ggaagcccca cgagatccat taaatagttt aacaaaccaa aattggggtc
    52201 ataaatccaa cgccagatta acgcagccgc taccggggat ataaccacag gagtgaaaat
    52261 cgccgtacgt aaaaaccgtt ttcctgcgat gcgtcggtta agtataatag cagttaacag
    52321 agcaattacc atatttaaag gaaccgtacc aagtgtgtaa ataaaagtat ttttcagaac
    52381 catccagaat tcaggatcag aaaataaccg acgatagttc tctaaaccaa ggaattcccg
    52441 cccactactg aacatattcc atttatgaaa actgagataa aacgagaaga ataccgggaa
    52501 taaaacaaaa gcgcagaaaa taaccaacgt cggagataaa aatccgagcg ctgtgagaat
    52561 attcttttta ctattcccat cccggcgatt tataatcgtc tttttaactc tggtagattt
    52621 atttagcatt ctgccaatac gattgtaaga ccttattaga tttcgcggcc gctttatcca
    52681 gagctgcctt tggatcaccg ccaccaagtg ttgcttcttc aatggcttcc gccatatgtc
    52741 gagtaatttg taaagcccca taatccatag agcgctgaat ctgggcaact tccatctggt
    52801 ctacaaatac tttaaaattc gggttttcta ttagatattt cttgaactcc ggtatttctg
    52861 atactgaccg ccgcaccggc aaataaccgg atttcatagc ccagcgagct tgaatatcag
    52921 gccgaatgat ccacttcaca aatgaccagg cttcatcggg atgttcagat tgtttgaaaa
    52981 taaccagata ttcgccaccg cagatggtcg cctgttttac aggacctttg ggcaacggtg
    53041 caaacgacca attcaaattt ttcagcattc gattaaatcg cgggaggttc caaggtccat
    53101 ccatcgccat agccaccaac ttcgaggcaa aagatacatc gtaatccgta gtaaatgtcc
    53161 gtaaattcaa tttgtcatat atgtttttcc agagtgtcag agccttaact cccgcttcac
    53221 tattatataa tactttagat tggtcttcag ctataatata accacccgct tgccataaat
    53281 agggaatcca ctgccaaacc atccagccac ttagagggcc cgcggctgga aatatgggca
    53341 gaaaaaatcc gatccggtca aatttcccgt cattattaaa atctttagat aatcgcacgg
    53401 catattcttc taactcttcc caattcgagg gagcacgacc atcctgatag ccagcttctt
    53461 taaccatatc aacattatat atcaacgcca ggttagtggc ttccattgga atacaatata
    53521 tcttcccccg cagagaagaa tattgtaata aagcaggata gatatcttct atttcggatt
    53581 tactaagtcc attgttgctt ttgataaaat gatccatctc ataaatcgca tccgcgtcaa
    53641 ctaaatcagc cagaaaatca gagtgaatcc aggaaatatc aggcgcggtc ttactctgaa
    53701 ttgccgtgat aagtttttga attagagcat caccagttgg cacatattga gcttttattt
    53761 tgacattggg attttcagtt tcatattgtt cgattaaatc ttcgagcgct ggtatagtag
    53821 atgcgacgaa gctatgccag aaagtgatgg tgattttatc atctttagta gcttcttttt
    53881 tcccacatcc aataaatact aataagcaaa acgatagtaa aaaatatcta ctccatcccc
    53941 tcagattggc tgtaaaaacg tttgcgcatt tctggggtga gtgttgattg ccgatatttt
    54001 tgaattgcat agttcggacc taatttttat tggttttaat cgggattaat aacagaattt
    54061 agatttcgga cggattttcg aatattgagg acaggtttta attcgatcct ggttttctct
    54121 tttcttccct tactttttaa atcgtcatac aacaatttaa ccgccatctc acccatgatc
    54181 tgccgaggtt gctgaatagc tgtcagcggg gtaaaaagat acggggcaaa atctacatca
    54241 tcaaaagaaa tcagcgaaac atcttgaggg acattcaatt tttcttcgat aattgcatct
    54301 aaggtaccca aagtaattaa atcgcttgta ctgaatatcg ccgtaggtgg attggacatt
    54361 ttcaatagta gtttggtctc aatatatcca ttttctcttc gataatcgtt accaacgatt
    54421 aaattctcat cgatttcgat attatattta ataagggcat ttttataacc tttaacccgc
    54481 atattgttag tagaagtatt gggaagccct tgtataattg cgatcctttt atgaccattt
    54541 ttgattaaat attcaacagc tttataggaa ccagtatagt tatctacaac cacagaattg
    54601 gtatcaatgg aatcaaaaca cctatccagt aaaaccaaag gaattttatc atcaatgagt
    54661 tcctggatat gatctgattt taaccccacc ggcattataa taaaaccatc tactcccttg
    54721 ctacgcagta attgtatttg ttcaatttca gtatcttgat tttcatcagt attgcacaca
    54781 atcaaagtat aaccaagttt ataagattca atttgaataa tgcgcgaaac atgagcaaag
    54841 aaggggtttg aaatatctgg aattaccaat ccaatagcat gcgtcttccg cagtcttaaa
    54901 ccacgggcta attgattcgg gacataattg agttcttgag caattttgtg aattttatcg
    54961 gcagtttcat ctttaatccg gtttactttc cctttattat tcaatacccg agaaactgtt
    55021 gaaacggaat atcctgattt tttggcaaca tcttcgaggg tcgctgtcat tttgaaacca
    55081 ctttgtaaat ctgcgcaatc attttcgcaa taagtatagt aaagaaaaaa atatttgtca
    55141 agctaatttt tcagagtatt acgaaatttt tctacgttca tctaataaat cattgatatc
    55201 attcaatata tagtatttta aatttattgt tacatacctt taaatcgcga caatctgggg
    55261 atgattttct acctgccgct aataagtcga gtcagattaa tattgttttt cttctaaccg
    55321 ctaagagcgc taagttttta tcaatgataa gtccaccagc caaacttata ctcgtcaggg
    55381 caatgagtgt taaccacaaa aaatactaaa tgacacatag aactgcataa atgctttttt
    55441 gcataattga ttgtggtaaa tggttactgt tagctggttt tggttatcgg tctattgtca
    55501 gtaaactatt tataaacccg attctattat tcgaattcta ataaaaacta ttttgttaat
    55561 tttgtctaaa atatatcccg cgccttttcg cgtggttcgt gggcaataac tatcatacca
    55621 ttttatttta ggacgatgct gacgatttta ttctttacca caaccgtctt aa
//
LOCUS       DAOOMU010000005        47282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_537_length_47282_cov_15.680670, whole genome shotgun sequence.
ACCESSION   DAOOMU010000005 DAOOMU010000000
VERSION     DAOOMU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 47282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47282
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_537_length_47282_cov_15.680670"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLI55_00940"
     CDS             <1..294
                     /locus_tag="PLI55_00940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA99437.1"
                     /translation="VLPSYYNEGVPKSLIEALSIGRPLITTDRPGCREVINDGKNGII
                     IQPKNVEQLVNAIEYMIQNPDERIRMGIESRLYAEIKFDVNKVNEIFFKYMNI"
     gene            500..1669
                     /locus_tag="PLI55_00945"
     CDS             500..1669
                     /locus_tag="PLI55_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPA99438.1"
                     /translation="MSNKLIKSIYLIWINYGSKLLHKYYHFLKRYKRDGFGMYVRILN
                     EDENIGYNNFIAESRFSSIYQSFEWGIVKQYDHWEVIRLIVIDSGQIVGGLSCLCKKS
                     PFFAKRIFYIPRGPVLDYFAENAPDIFKLLFLKIREIARREGALYIRISPDISKNIHF
                     NDYISKYNLHRVRYPILHTTTYRLDLTKSEEQLLNSMESRVKYDIRKAQRENVEILSD
                     DGNEEYLKDFYNLLYQVAERNKFPIYSYEMMQNIWNILSPNGMCKIFLARSQGKIISG
                     AFLFLFGDKCIYQWGGSVRAKNNPNQLLHWEIIRWAKSNGFSIYDLQGIPENVKKGDE
                     LWGIYLFKRGFGGDRIQLIGEYDYILSPILYKIWQVIEPKYPAVKNCLSKIKKRK"
     gene            1666..2439
                     /locus_tag="PLI55_00950"
     CDS             1666..2439
                     /locus_tag="PLI55_00950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPA99439.1"
                     /translation="MSKRLILAYHRILPDELALLRQPLAVSTTQFERQILYLINKGWR
                     SLTLSEYFEIYYLEQKKPDRVFVVTFDDGYRDNYYYAFPILKKYNLKATIFLVTGLLN
                     EPKTLQSDNNINNNLTEMDYSITLEQMREMANYGIEFGSHTVTHPRLDTIGLAEAEEE
                     IYQSKRMLEQYYNRQVKTFCYPYGAFNDDVISLVAKSGYQCAVVTPTRPGIKETRFTL
                     IRVGVYNSNSLLKFKFKCSNLFTNIRKARLWFLLKHLLT"
     gene            2741..>3702
                     /locus_tag="PLI55_00955"
     CDS             2741..>3702
                     /locus_tag="PLI55_00955"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPA99440.1"
                     /translation="MVLASLLAVFLTTYICALGLTYLARKIALRYHIGDMPSERKVHK
                     VFTPYLGGAAILLSIIIGIVLSIYLLPMVAEEVPRRYYFLGITGVLIALVGLYDDIKK
                     LRFSTKFYFQILAAIIVVLGGLRFESLYIPIIGEVGLGWFSIIFTILWIVFITNAMNL
                     LDGLDGLAAGVSTIIFLAFALVALHSGRTLVALVCLLFIAANLGFLKFNSHPASIFMG
                     DTGSLFIGFSTAVISLEVGRIGHTNCLDIIIPLTILAVPILDTTISFFRRINKHMNPF
                     MADKEHIHHRLMSIGFSHKNAVKVMYFLSIIAGFIGCSFIYLDNR"
     assembly_gap    3703..3802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3803..5146
                     /locus_tag="PLI55_00960"
     CDS             <3803..5146
                     /locus_tag="PLI55_00960"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPA99441.1"
                     /translation="FLAVYYWQIVPNLLPEQLLTPYNLILWIAWSVIYWTSLLGLNNL
                     YRIEWDTSRVDEIFTVFKIIFFGTIVLFFLSFEFKIPFLPSRRILVTYGIFLVTGISV
                     GRLVLITILKKYNLLEFKKRPTLIIGASRRALNVVRKINSVPELKFNIVGMVDDHANG
                     NIGKIIEGVPIIGSFENIPEIIKKRKIKEVIIALDDNNMNQVIDMIDLFSRYNVSIKL
                     LPDFYNLLTGFRTSHIYGVSLVKFFGSNMKTWEWVLKRLIDIIISLVVLIGFLPIWLI
                     ISLIIKLDSPGPVFYKQKRIGKNRREFEVIKFRSMVQGAEQETGPKWAEKNDPRVTRF
                     GKFLRKTGLDEIPQFFNVLMGDMSIVGPRPERDYFVNELERSIQFYSRRLIIKPGITG
                     WAQIKYKYDETIDDVREKLRYDLYYIENMSVILDLKIIIQTLLIGLRRKHRTSYV"
     gene            5239..6516
                     /gene="serS"
                     /locus_tag="PLI55_00965"
     CDS             5239..6516
                     /gene="serS"
                     /locus_tag="PLI55_00965"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPA99442.1"
                     /translation="MLDIKQIRNEPEKFRRGLEAKNAGDKLDELLKWDGERRKCINQA
                     DELKAIRNRVSDEIGQLKKKGLPVAEKILEMRSVGDQINNLDHKLKEIDEKINSILIT
                     LPNLPHPSVKIGKSAADNVTIKEWGHKPEFDFPLKDHLDLGEALGILDMKRAAKISGS
                     GFPMYIGDGATLERALVNYMLDFHRNHHGYQEVFPPFMVNRESAFGTGQLPKLEEDMY
                     HIEIEDLFLIPTAEVPVTNIHRDEILPENQLPIKYTAYSACFRREAGSYGKDTRGLSR
                     VHQFNKVELVWFTTPENSYQVHEQILRDAEDILQALNLHYRVVELCSADLSFAAAKCY
                     DIEVWAPASGKYFEVSSVSNFEDFQARRANIRYRRTSDNKVNFVHTLNGSGVATPRLM
                     IALLETCQTPEGTIIIPQVLQKYTGFNQIRTKK"
     gene            6513..7169
                     /locus_tag="PLI55_00970"
     CDS             6513..7169
                     /locus_tag="PLI55_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPA99443.1"
                     /translation="MTGEKKYRKLICEIGKKIYANGYVAANDGNLSMRLNAEMVLITP
                     TNVSKGAVKPSQIAKIDLSGNPLDKNRPSSESLLHLAIYKRCDWVKAVIHSHPPYSTA
                     WAVTGRGLTDPILPETIVSIGQIPLIPYKPPSTQELADIVADAAQKNDVLLLQNHGLV
                     TVGKDLLDAYYKTERAEHVFRIVALAKQIGEIRTLSKAEVAELYRLFNVSEKLQVTYR
                     "
     gene            7177..7890
                     /locus_tag="PLI55_00975"
     CDS             7177..7890
                     /locus_tag="PLI55_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPA99444.1"
                     /translation="MIRNFFAVIWVVLVTIVFGSLSMALPGSHNTMMRKWSQMILQFL
                     GVKLQVHGLENIVNRGSKIVISNHESALDIPILMAALPVPIRFMAKKELFQIPLFGWT
                     LNLARHIPVDRKRPRKSYTVINRVAKELIRNGLSIAVSPEGTRSYDGKIAPFKKGAFR
                     LAQIHNLPILPVLILGARHCVPNKSLSIIPGIVEVHIGKEVHLSDYPTMNDCVADLRN
                     QMIVQKEQYEEKRKQGSLD"
     gene            7883..9238
                     /locus_tag="PLI55_00980"
     CDS             7883..9238
                     /locus_tag="PLI55_00980"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPA99445.1"
                     /translation="MINCIPNRIAEPEISITKKEKSLYKAWLNQEFNLADLQTVEGYS
                     LSILSPGNRNETEGPDFHDALIMLNEKFERGDVELHLESNDWYSHGHDRDPHYNNVIL
                     HVVLNSGKLLPIKSCDNRIIPTLIMTPFAISAPDEYACLSWKHLSNDDLNQVLKTFAE
                     IRFKRKAQIWRSLILTKGAEQTLYEGLADVLGYSRNRKMFGILSQKVTLELIRKILRD
                     TDPEQRIIVLESLLFGSAGFLTKPELKVFIRDTKYSEGLYKIWVKISQKYELEELEST
                     DWHFAGSRPPNFPTLRIAALAQIINKFYPNYLAETWLRVIQNAKNFTDIRRYASEMFQ
                     QPDGLWRNHPLLKQQPGKMLIGVQRFNDLLSNLLLPFSWAIASLDNQYELIEKTTTFA
                     EQVECLEVPGSVKVALNRLPLDTITLHQNYLTQGIIEFSRRYCNMNICKLCPLEKYAQ
                     K"
     gene            9225..9704
                     /gene="rfaE2"
                     /locus_tag="PLI55_00985"
     CDS             9225..9704
                     /gene="rfaE2"
                     /locus_tag="PLI55_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPA99446.1"
                     /translation="MPKNDKIITREQAGRIVSQLKREGRKVAFTNGCFDLLHIGHLEL
                     LEHARKLGDILIVGLNSDDSVRRLKGPQRPINTQLDRARILAALEVVDYVVIFNEDTP
                     LELIQTIKPDFLIKGGDYSPDTIVGSAFVESYGGKVIVFPLVHGKSTSDLINTIFQL"
     gene            9777..11582
                     /locus_tag="PLI55_00990"
     CDS             9777..11582
                     /locus_tag="PLI55_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPA99447.1"
                     /translation="MNNRKKLRLILLFWMTITISVALSANNSYEKINNNNGEEKELSD
                     NSGNDIFELIFNEAKLFYVDALVSSYYNDTAAVKYCFDRIFEIIAEISELDSLTLVEQ
                     DDFNRFNEMVSNDYQTHFSYLIEETDSVMIVSVGEELFVTPLDTVDIGNDTLIVVEDR
                     PGHIPIVRSKKIDKLIIYYSGQQAPVIQRYLEAFNKYKDHYLPILKKYNVPEEIIYLP
                     ILESGYNPNAYSYAHAAGIWQFIAGTGAKYGLKRNWYVDERRDPIKSAHAAAKYLRKL
                     YEEFDDWYLALAAYNCGEGNVWRAIRREGTRDYFKLRSLPSQTRNYVPSFMAITIIAK
                     NPEKYGLTTPTTPTWEWEEVIIDRCYELDVIAKACETTPDILRQYNPELRRWMTPAND
                     NQYVLRVPKGKAEGLTEKLVSLPEPKDVKPEVVYHKVRKGENLSSIARKYGTSVSALV
                     SANHLRNRNQLRVGQVLLIPTGSYYSAPEKKSSQTNVVTHVVQRGETLSEIAEKYNTS
                     VSKIRSMNNIHNSHIQVGQKLKVPTNLASQIEPSDNPKGSTKVIHVVKKGDTLTSIAN
                     KYNVSLTKLKSWNDLDGRKPIYPGQRIVIYQSIKS"
     gene            11710..12606
                     /locus_tag="PLI55_00995"
     CDS             11710..12606
                     /locus_tag="PLI55_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HPA99448.1"
                     /translation="MNSINISLMSNVGHLILTMVSSEILNTERHRDGVKYFCKLFRIE
                     SSLPSINHVTSILRKFSLLPYENISKILSLNQNWDENHFRWPQEIICDYEKYKLGGTC
                     FSLTFFLKAILDYFGYQTTILMADMKAGRNTHCALLLEFKNRKYLLDPGYLLHFPLPL
                     NSAVISEKVQLEYDPFCERYSLSTLENGKKKWRYSFQERPTSIEDFSQYWEDSFHWMT
                     MHGICLSKRDENNFIYLHNHYLKLENRSQHFKGNLNTEIAELAQRWFNIPPEIVRQAE
                     KALRENLYQDKILGYQIPKWFK"
     gene            complement(12743..13609)
                     /locus_tag="PLI55_01000"
     CDS             complement(12743..13609)
                     /locus_tag="PLI55_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPA99449.1"
                     /translation="MTNYSSSSRKSPFISNEVLRRYFDEIRTEKTLTPDEEIELARKV
                     KAGDTQALDRLLKANLRFVVSVAKKYQGYGLSLADLINEGNIGLIKAARRFDETRGFK
                     FISYAVWWIRQTILQAISENSRLVRLPLNVVGNLNKITKITSEFERDYEREPTNDEIE
                     VLLQNENIDIDSARQLTENTISIDAPVGNDQTGTLQDILPSQEDNDPGLTLNQESFHL
                     EIERALNSLDKREAYILRLYFGIDSDLPLNLEDIAERLHLTRERVRQIKEKALRKLRH
                     NSRALHLRGYLG"
     gene            complement(13637..14029)
                     /locus_tag="PLI55_01005"
     CDS             complement(13637..14029)
                     /locus_tag="PLI55_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99450.1"
                     /translation="MTGIWDDIKKSVGGFASKAAEKAGELSREAAEKAEEMTQLGKIK
                     LDIFQIKRDIDKNYTELGRVVYGLLNQNTKGNLVKNEKVTSLINNIKELEKKLKDKEA
                     QYYKIQESSKENTAKPDEPDQSKSETET"
     gene            complement(14162..15841)
                     /gene="groL"
                     /locus_tag="PLI55_01010"
     CDS             complement(14162..15841)
                     /gene="groL"
                     /locus_tag="PLI55_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561033.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPA99451.1"
                     /translation="MAKDITYEIQARTALKEGVDKLANVVKVTLGPKGRNVVIEKKFG
                     SPTITKDGVTVAKEVELEDKLENIGAQMVKEVASKTSDVAGDGTTTATVLAQSIIAEG
                     IKNVTAGADPMELKRGIDTAVNTVVESLKELSKAVTNSKEEIAQVGTISANNRKVRRM
                     IKDESGKEDIVEIPIGVLIAEAMEKVGKEGVITVEEAKSTLTTLDLVEGMQFDRGYIS
                     AYFVTNAEAMEAVLEDPYLLIYDKKISNMKELLPILEKVSQMGRSLLIIAEDVEGEAL
                     ATLIVNKLRGTLKVAAVKAPGFGDRRKEMLEDIAVLTGGKVISEEAGYKLENATISHL
                     GNAKRIVVDKDNTTIVEGAGKPEDIKGRIKQIKAQIEASTSDYDKEKLQERLAKLSGG
                     VAVLKVGAATEVEMKELKALVEDALHATRAAVEEGIVPGGGVALIRCIPALDKIKVTG
                     DQMVGVKIIKRALEEPLRQIAKNAGHEPSIVAQKIKEGKDDFGFDAYKEEYVHMYKAG
                     IIDPTKVTRIALQNAASIAGLMLTTEAVITEVPEKEPAAPAMPPGGMGDMY"
     gene            complement(15863..16147)
                     /gene="groES"
                     /locus_tag="PLI55_01015"
     CDS             complement(15863..16147)
                     /gene="groES"
                     /locus_tag="PLI55_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPA99452.1"
                     /translation="MAIKPLADRVLVKPLVSNEKTPGGIYLPDTAKEKPQEGTVIAVG
                     PGKTTDDGKKIPLEVKVGDHVLYGKYSGTEVMIDGEEHLIMRESDIFAIL"
     gene            16399..17178
                     /locus_tag="PLI55_01020"
     CDS             16399..17178
                     /locus_tag="PLI55_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPA99453.1"
                     /translation="MISSNFLFILVGFVAGVLSGLFGIGGGIVIVPIMIWFCHFSQLA
                     ANSISLTALLLPVGILAVLAYHKSKLVKVRQALFISLGIFIGVVGGAILALEIPAHFL
                     KRLYGIFLLYIAWTYIDIPSLFANGANQEKTNQQFENLNLDRPVCYIVLGFAAGIMSG
                     LFGIGGGIIIVPALISIFKYPYKKAVGTSLMALLLPVGLPGVLIYNRAGQLQIGPALF
                     IALGLLFGALVGALVAIELPTKLIKRIYGGFLLLAGFNFIF"
     gene            complement(17221..17568)
                     /gene="rplS"
                     /locus_tag="PLI55_01025"
     CDS             complement(17221..17568)
                     /gene="rplS"
                     /locus_tag="PLI55_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002833579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPA99454.1"
                     /translation="MDKLTAAVADQFRTDIPEFKPGDTVAVEVKIIEGDKERLQTFQG
                     VVIARHGSGISETFTVRKISNGIGVERIFPLHSPKISKIKRISVGKVRRAKLYYLRGL
                     RGKASKIEESNRR"
     gene            complement(17561..18271)
                     /gene="trmD"
                     /locus_tag="PLI55_01030"
     CDS             complement(17561..18271)
                     /gene="trmD"
                     /locus_tag="PLI55_01030"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008403309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPA99455.1"
                     /translation="MKIYIITAFPGMITACLSESMFRKAVENGKVEFNVWNLRDFTRD
                     KHKQIDDKPYGGGAGMVLKPEPFFRAYDKIVKLTGHKRPRVVFPTPQGRLFNHHLALE
                     LAQESDLTFFCGHYKGVDERVIANLVTDEISIGDYVLTGGELPALIIIDAVIRHIAGV
                     LHSYESAETDSFTDNLLEGPIYTHPQVYRRKSVPEVLTTGNHAKINAWRQEQRKLRTR
                     QRRIDLIKNMNMEDKNNG"
     gene            complement(18268..18774)
                     /gene="rimM"
                     /locus_tag="PLI55_01035"
     CDS             complement(18268..18774)
                     /gene="rimM"
                     /locus_tag="PLI55_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPA99456.1"
                     /translation="MDHLVLLPVGSILKSFGLNGAVLLNRGSLKNIKVDEPKVLWLGE
                     SAQVARPWKVDYLRISTKKVYLKLRDINSRQEADYLRGLKVFVPREYELKKELVELIG
                     FRVRVINSAKDIGFISSLDETNPQALFVIQSEDATLLVPAVTDLIEQIDPEQQIVIFK
                     DIEGLFPK"
     gene            complement(18783..>19018)
                     /locus_tag="PLI55_01040"
     CDS             complement(18783..>19018)
                     /locus_tag="PLI55_01040"
                     /inference="COORDINATES: protein motif:HMM:NF002201.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPA99457.1"
                     /translation="MKEFVEFIVKQLVDHPEDVKVVQVDSEKTVILELSVKEGDLGKV
                     IGKKGRTARAIRTLLTAAAAKQGQKRAILEIIE"
     assembly_gap    19019..19118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(19287..19523)
                     /gene="rpsP"
                     /locus_tag="PLI55_01045"
                     /pseudo
     CDS             complement(19287..19523)
                     /gene="rpsP"
                     /locus_tag="PLI55_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(19541..20866)
                     /gene="ffh"
                     /locus_tag="PLI55_01050"
     CDS             complement(19541..20866)
                     /gene="ffh"
                     /locus_tag="PLI55_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPA99458.1"
                     /translation="MFEQLQNGFGAIIKSLRGEGRITENNIADAMRQIRRVLLEADVN
                     YKVAKEFVQSVQEKSVGVTVAKSLSPGQLIVKIIYDEMVHLLGDKNRNLKEASIPPTI
                     IMLVGLQGSGKTTCAAKLAYQLLQKQHSPVLVPVDVYRPAAEEQLEILARQIKVPCFR
                     ENPDVVGRIQDAITFARQQHRDTLIIDTAGRLHIDEKMMAELKTIKNTIKPHNILLVV
                     DGMTGQDAVNTATSFNEQIGVDGIILTKMDSDTRGGAALTIVSSTGKPIIYMSVGEKI
                     GDLEIFHPERVAGRILGMGDVVSLVEKVQASVDLESALKLENKLKKEQFTLEDYLTQL
                     RQLKKLGPLDSVMEHLPGFARMRLGNQKVDEKELIKAEAIIQSMTRTERLKPNIINGS
                     RRRRIALGSGTQPQDVNRLLNQYWQITKLMKQMGRMKLPKNLSAFGIGN"
     gene            21067..22089
                     /locus_tag="PLI55_01055"
     CDS             21067..22089
                     /locus_tag="PLI55_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HPA99459.1"
                     /translation="MNRRHLMIVLTFPIFLMYNCSETQPESSNPYRINSGLNPGINIG
                     NALEAPNEGEWGVVIKDEYFPLIKSAGFNSVRLPIRWSAHADINPPYTISDDFMNRVR
                     HIVDTALEHKLYVVINVQHFQEIYTEPIENKPRLLAFWHQIGLAFKDYPQTLLFEPLN
                     EPHFNLTPELWNEWIPELIDTIRVTNPNRTLVIGTADWGGIWKMKELKLPTTERNLIL
                     AIHYYEPFRFTHQGAEWEKESNAWLGRTWTKTTDQMQELNQHFDLIKKFADSLGVPVY
                     IGEYGCYNRAPEESRLIWTRTVSEQCEKYGFSRAYWEFCSSFGIYDPISQQWREPLRS
                     AVLLAR"
     gene            22365..26282
                     /gene="porU"
                     /locus_tag="PLI55_01060"
     CDS             22365..26282
                     /gene="porU"
                     /locus_tag="PLI55_01060"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPA99460.1"
                     /translation="MIINKRFCAKIIFTSFCLFSAMLVRLEAVELTILSETTSELILR
                     VEMDADLEWRQIENYPNKVSALTLGDAYQSHLSQTLEIPYYQWLIALPTEDKPSVSIT
                     KSVFESVKLLKNLTTDELTALDKFPLVAIAETGFLRDVPFGSLVIYPLRSANNSNELR
                     LLREIEITIQYTAVRKTTINQTTVEKGDYLNAFVNGSSATQWRQSRPLSLRKPQSQPA
                     GKWFRLTVKKDGVYTLTLDDLKSAGLTETSIDTGRIYLYSNSTGGRPLDATIGAAVPS
                     NLIENTRWIKGNNDQDFTNADTILFYGRATSGVDLDSKNSLTFVRNPYSLINYYWLFI
                     ADEPGYPKAMPTINSLSATPNMTVTQSEHLEVHEEEKVNFLRSGLKWSGEKFNTSGSN
                     VSVILTMPKVRDSYVGVMTICTRGAVDEILHYFKAYLNNSTTPVASWSATNYQSATTS
                     FSTTLSANINILKINYTSQPSSASAYLDYVMFRYTKDLSAFTEPQTFWSLLYSGIVEY
                     QITDLNFTNLLILDVSDWSNVSIQQYNRNSNLISFRTASQSNQRRQYYLTAPSFFNKP
                     EKIELIPNPQWITLRNITSSYDYIIITADDFLTAAEKIATLYSQELSPGDRLNTLVVT
                     QNQILREFNADITDPHAIRFFLKYAYENWTPAPAYVLLLGDGTFDYRAIESSTGNYVM
                     TYQVEPAVEDYSGFSSYASDARFVYIKGSDQVMDMAIGRVPVRTATEALDYYEKLRQY
                     LTEPIYGEWRSRITLVADDPQRPYNNEKYHINDTESYIAKYIPKSLITDKLYLLEYPE
                     VQDVSSNWVKKPAATEAILNQLKRGTTLINYLGHGSSTVWAQEEVLKMDRDIGLIEAG
                     MKLPLWIAGTCSWGQFDDINTICMPEALINKNSDGAIAILTPTRPTTGTQNASFIGNF
                     IKKLFPSGEISRTRLGALIQNIYNGTNENQEKYVLFGDPALYLALPYQKAAFDKMPRD
                     TLAALEKVSVTGRTVTEENFNGDGIINVFDSDRYVTRYYLDDAKVTQNLSYTLSGETL
                     YRGNLKIENSKFFSRFYIPKDLSYMNLFGRIKVYGWNTSAMSDFAGCYDSLLFTGTTQ
                     FDDTSGPQIVIGFEGMDFKSGDIVAPDAKIKVSIMDPVGINIAGKLGHDITFRFDNDN
                     DLIFEMTEDFVYDVNSDSSGVITSDLPDLSPGIHQITVKAWDNANNYATATAEFILAS
                     SDKLKLERVVNFPNPFASTTDFVFYLTQPGQVKITIYTIRGLKIKTIASDEILPPGFH
                     QVNWDGCDDFGDEIARGIYLYKVQVQSSDSNQKDTFIGKMVKS"
     gene            26279..26878
                     /locus_tag="PLI55_01065"
     CDS             26279..26878
                     /locus_tag="PLI55_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HPA99461.1"
                     /translation="MTTQLNWYSMPIILASQSPRRKEILDMIGLKYEVCPPHYIENNS
                     IGKSPTTIVIKHALNKARTVAPRYPKSWIIGADTVVVCKNRILGKPQNENEAVQMLKF
                     LSGVEHSVFTGYCVLNSANGRYLKNAVRTRVRFKEIPLAEIQYYVENYHPLDKAGAYA
                     IQDFSAIFVEKINGCFYNVVGFPLPAFYEQVKNELSICL"
     gene            26946..27725
                     /locus_tag="PLI55_01070"
     CDS             26946..27725
                     /locus_tag="PLI55_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HPA99462.1"
                     /translation="MATFYEILKQSREAQGLTVEQISSRTKISKEIITAIESGDLKIL
                     PKTYMRLFLKAYSQELNLDPEAILKNFEESLGEPVETPAVPDNNIKPSLPISQKPEKP
                     EKPIIGKKQNRNFSTVIIVFVILVFLIAVLRQVLMDEKKTSSNTALPKITPTDTTQTI
                     PPSPDLEVAVPKSELNLTITTKDSCWVRIVIDNRTTFEATLPPLYKKEMIAKEQFDMQ
                     IGRPASINLVLNGKDLGPVGIPGIPTRLIITKDGIIRRQSF"
     gene            27896..28348
                     /gene="mraZ"
                     /locus_tag="PLI55_01075"
     CDS             27896..28348
                     /gene="mraZ"
                     /locus_tag="PLI55_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPA99463.1"
                     /translation="MITAFAGETRHPLDEKNRLSIPAKYRRWADGQDGECTFVVTKGS
                     DPCLVVYPSTEWDVLSEKLLKLSYFKKKNRAFIRALARSSVRLKCDGQGRILIPTSLL
                     EYAHIKKEVTIIGVLNRLELWDPETLEKHEESSIPLDDEYYEDLGDIL"
     gene            28373..29296
                     /gene="rsmH"
                     /locus_tag="PLI55_01080"
     CDS             28373..29296
                     /gene="rsmH"
                     /locus_tag="PLI55_01080"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPA99464.1"
                     /translation="MTTLHIPVLVEQTIQFLINRPTGIYVDCTLGTGGHFQAIAQHLD
                     NDAYLIGIDADPSAVQYCEQNISISQPHQFITANFADIKRICYRAGFPKVDGILMDLG
                     LSSFALDNPERGFTYSQAGPLDMRFSPDTAQTAMDFINTASLKELVLVFKEYGEERYS
                     QNIARRIIADRDKNPIKTTGQLASIIREEVPKQFQIKTLSRIFQAIRIQVNQELDALR
                     QGLSDAVSLLAPSGRIVVISYHSIEDRIVKQFFKKEASDCICPPDFPICQCNHHAQLK
                     ILTHRPIVPSKSEIERNSRARSARLRAAEKL"
     gene            29306..29620
                     /locus_tag="PLI55_01085"
     CDS             29306..29620
                     /locus_tag="PLI55_01085"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HPA99465.1"
                     /translation="MSSRKSFRKKKKNLLLIIGFFVLVGSMVFYIWIYNTTNALYREI
                     DQLKRKEASLIARNQMLVVEIERLSRADRIKNIATTEIEMVTPLPETLAVVIDSKVLA
                     VE"
     gene            29620..31623
                     /locus_tag="PLI55_01090"
     CDS             29620..31623
                     /locus_tag="PLI55_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPA99466.1"
                     /translation="MANQFKSIRKRTLIVSSSLVVLWVVLIARLFYIQVLNSKKYQEL
                     CNNQANLRKAVLPRRGIFYDRNGEALTIDLVNYSIAAHPYLVEDKPRLASTLANDLSA
                     KPEQYLKLLESNKTFVWLEREVPYSSFQNYEKYEKTPGLVIEKNVKRYYPLGEITGQL
                     LGFTNIDNRGVCGLESGLEGLLCGTPGWIMVQKDGWGRLHYRPDLPSKSSIDGNDIVL
                     TIDREYQTILYEELLQAYTETKAEKAMGIVINPNDGDILAMVTLPPFNSNKPSDYPIS
                     AQRNAVVSDIFEPGSIFKIVTATAALDNKVLQPKHQIDCNPGFIQVGNRIIRDHDKYS
                     ILTFAEVIKNSSNVGTIKVAQLTGKDQIFNYARKFGFGAKTDIQFPGEESGILHPFKK
                     WNDLVLAQVAIGQGVCVTALQTGMAYAAIANGGLLLKPQIVKTIKTKDGIEIFNAQPV
                     YIRRVASSETMSTLRELLRLTVMSGTGSRADINGLAIAGKTGTAQKVVNGAYSQTEYV
                     ASFVGFFPADNPKLLCVVVIDNPKGLRHTGGGVAAPVVREVFKRIVNQSDDLFFDEPD
                     PLPTPVVNYVYETKPAVANVPQTPDEAYHPNLTTAIYTNQMPDLCGKTLRQAVGILYE
                     LGIDDIEIDGAGVVVSQKPTPHSPINPHVKCILKLQPTRTPLE"
     gene            31616..33079
                     /locus_tag="PLI55_01095"
     CDS             31616..33079
                     /locus_tag="PLI55_01095"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPA99467.1"
                     /translation="MNNSLSEILQGIEFRRLGNLPTSVAALSVNSKELAKGALFFAIR
                     GAKDDGHKYVKNAIQNGAAAVVVEEVVADESIPQIIVADTKTALSKAAANFYDNPTNN
                     MTIVGVTGTNGKTTTVYLLNEIFKEAGLKHGTVGTLGYSIGDERFSSNLTTPDSIQLQ
                     QIFKKMLNRGVEIAAMEVSSHALALHRVDDVHFKAAVFTNISQDHLDFHHSLDEYATT
                     KSKLFAMIEKDGFVINNLDDNYSDLFKNAARCRLFTYSLLKTADFTWAPGVTWSNGIS
                     GEILTPNGKIEIKSCLSGQFNLKNILAATAVAVNLNIDDKIIASALSGVESVPGRLQE
                     ISHSGQLRVFIDYAHTPDAITNVLQTLRGMLPADGRLIVVFGCGGNRDRDKRPKMARA
                     VESLADQAIVTTDNPRFEEPHDIIEDIVKGFTSGYNFKVIIDRKEAILDALKNAEQND
                     IIAILGKGHETYQEIRGVRYPFYDGEIVQQFYGGSND"
     gene            33072..34421
                     /gene="murF"
                     /locus_tag="PLI55_01100"
     CDS             33072..34421
                     /gene="murF"
                     /locus_tag="PLI55_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPA99468.1"
                     /translation="MIKADFIRSLGLGRIVNFEGAINGAEIDSRKLQPGAAFFALKGA
                     TSDGHAYVQEALQKGAVLCVVAEDWAAKNPTPAPLWIVPDPEKALQKLAEKWRLSFSI
                     PVLAITGTNGKTTTRAMCAAIIKTAYNLHTTSGNFNNQLGLPITLLNLRPEHQFSLIE
                     MGTNHFGEIARLCEIAHPNAGLITNVGWGHIEFFGNIEGVAQAKSELFAALPENGTAF
                     INADDPLIVKMPVAGRKIKYGFENKAVDFYGKISGYNDEGQAVLNVNSRFDIHLSIPG
                     RIAALNALAAVAVGLFFNVDEKAVIAALEAFQPVSQRFVQIKVGPYQVINDAYNANPD
                     STIAALETFRRIKTSGRRIFIMGDMLELGQYAIQGHKRVGKAVAQADIDRFYGVGTLT
                     AYAIETAARAGLKAAYYFSTKSDLLAALKSDLKEGDTLLVKGSRGSRMEEIIEGLSQ"
     gene            34423..35526
                     /gene="mraY"
                     /locus_tag="PLI55_01105"
     CDS             34423..35526
                     /gene="mraY"
                     /locus_tag="PLI55_01105"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPA99469.1"
                     /translation="MFYHLLYPLKKYFTGFNLFQYITFRAAMAAITALLISFIIGPYI
                     IKKLKKYQIGELIREDGPQTHLAKKGTPTMGGIIILFSVIVPVLLWANLTNTYINLIL
                     LSTIWMGIFGFLDDYLKVIKKKKKGLIARYKLLGQITLGLLIGCYLYFSPEFADINSI
                     TSVPFLKNNYLDFGWFYIPFVIFFITGISNAVNLTDGLDGLAAGLMGIVALAMAVIAY
                     ITGRVDFSNYLNTIYLAGSGELSIFCMAAAGAFLGFLWFNAKPAEVFMGDTGSLSMGS
                     ALATLAILLKKEILFIFLGALFIAEALSVIIQVVYFKWTKKRTGVGQRVFKMAPLHHH
                     FEMQGIPESKIVIRFWIIGILLALFSLSTFKIL"
     gene            35523..36917
                     /gene="murD"
                     /locus_tag="PLI55_01110"
     CDS             35523..36917
                     /gene="murD"
                     /locus_tag="PLI55_01110"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPA99470.1"
                     /translation="MIDLKTKPANLNDLKVAVLGAKRSGIAAAILAARRGAEVLLSDT
                     ANPEIAPEQHSALKQHNVQLEIGGHTSKIFDSDLVVLSPGIPNDAPIVIEVTKHSIPI
                     VAEVEMAYWFCPTDNIIAVTGSNGKTTTTTLLSEIFKGTQYEPYCGGNIGTAFSHLII
                     EAERSAHPQKIFILELSSFQLERIVHFRPKVSLILNITPDHLDRYEHRMELYLAAKLR
                     ITMNQTEDDYYIFNDDDELLKTNLPPRCHLIPAGLHSTSQKLITSDAEKIYFSGQDQF
                     IERARVALLGEHNLYNLTAALTAGRLYQLPITHLQNVLYNFKSIEHRLEFVTTIDGVD
                     YYNDSKATNVDSVNYALKSFNRPVIVILGGKDKDSDFTLLIPQIREHVKAAVLIGKAA
                     DKIRKALSGVVEMYDAGYSMPNAIEIARNLAAPGDIVLLSPACASFDMFNDYEHRGRV
                     FKEIVMNLPKDLKV"
     gene            36914..38056
                     /gene="ftsW"
                     /locus_tag="PLI55_01115"
     CDS             36914..38056
                     /gene="ftsW"
                     /locus_tag="PLI55_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPA99471.1"
                     /translation="MNVAFKKGKVDQKYLIAFFILLGFGLVMQYSASSSLGEARFDDP
                     SFYVRGQFVRIVLGMVVGLICTFINYQILKKGAFWIALAGIIALIATLVYHKLHPGQA
                     TARWFPIGGFNFQPSEFAKLALIIYLSSFIERHERHLDDFKRGFIPPVIIMLIMVILI
                     IVEPNYSTAAVFTVFGLIVLFIGGTKLLHFLPFIGGFALLSVITILRSPYKLMRILTF
                     LEPEKDLQGAGYQIHQSMITLGNGGFFGRGLGGSIEKNLFLPDMHTDFIFSVVGEELG
                     FIGAVVLLSLYLYLFIRGIKIALRAPDVFGNLLGIGLSSCLFIYVIVNVGVTCGLLPV
                     TGLPLPFISYGGSSMLFNFACVGIILNISRYAVPQAKTNSLVMFND"
     gene            38049..39188
                     /gene="murG"
                     /locus_tag="PLI55_01120"
     CDS             38049..39188
                     /gene="murG"
                     /locus_tag="PLI55_01120"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPA99472.1"
                     /translation="MTKKDESRRLKVLITGGGTGGHLFPAIALAEELQMRQSQIGELE
                     IIFIGTRRGLESQIVPSLGFEFHKIWIRGWQRGWTLNDILVNLLFPVRLFVSLIQSFF
                     IIKNFDPDLAIGTGGYTAGPPLRIAAMLKIPIFLHEQNVIPGATTKMLANKAKRLYTS
                     FEESKTYLENAICLGTPLRRSLQIVPREQAIYFFELEPDRKTVFIFGGSQGSQAINSY
                     WIENITTVVEAVKCQFIWQTGQRDFKMIDNIFSNNPWVHVTPFIHEMGIAYCAADVVI
                     SRAGALSLAELCLYGKPSILVPLPTAAGNHQEINARNMEQRGASILVLQKELTNDRLT
                     QELIALLKDEPRLQAMSANAKALARPDSAKLIIDDILGFIEVNAL"
     gene            39178..40536
                     /gene="murC"
                     /locus_tag="PLI55_01125"
     CDS             39178..40536
                     /gene="murC"
                     /locus_tag="PLI55_01125"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPA99473.1"
                     /translation="MHSEVKKYHFVGIGGIGMCGLAEYLLHNGQIVTGSDLAPSENTE
                     RLENLGAQIKYGHRRENVGDCDYLVYSSAVKPDNPERMVAIERQIPTLRRAELLGQIF
                     DTKPIRIAVSGTHGKTTTTAMIGQVLTTAGMDPLILSGGVLKSSGSPVQIGKGDIVIA
                     EADEFDRSFLQLSPTHAIITNIESEHLDCYANLTQIKNAFYEFTAKIPDAGRVIACSD
                     EKEIPDFLKHHPRPYVTYGLYSGEFQARNIQLKGNSSECEVYHNSDLIGSLQLHVPGL
                     HNIKNGLAAIAMASLLNIPFPVIQEGLADFRGVKRRFEILQENDNLMLVDDYAHHPSE
                     IAATLNAARKGWQRRIIAVFQPHLFSRTRDFYPEFARVLRVADVIVLLEIYPAREAPL
                     EGISSRLIYDEICKDRTQDVYLTNTLAEALALLQEIKLTGDMIITLGAGDINKILLKL
                     ME"
     assembly_gap    40824..40923
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <40924..41463
                     /locus_tag="PLI55_01130"
     CDS             <40924..41463
                     /locus_tag="PLI55_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HPA99474.1"
                     /translation="QIQNRLEMEPYIEAAVVSREFPNHLKIMIKERIPVCYLNTGTLW
                     LIDQQGIILPLRKEKLKANLPVITGFEKDSARCRPGQLAPNPEMFKALNFIQTAALKA
                     NTLYTEISEIFAWKKGEFIIYTVNGGTPIYIGRGDLTKQLYILAAFQNLIKSKRTFAD
                     YQYLDLRWDKQIIAKERKS"
     gene            41464..42735
                     /gene="ftsA"
                     /locus_tag="PLI55_01135"
     CDS             41464..42735
                     /gene="ftsA"
                     /locus_tag="PLI55_01135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPA99475.1"
                     /translation="MKMLKLKNQILTGLDVGSSKVCAVIAEVTPDSPEPIIKGYGISP
                     SKGIKKGLVVDIENATQSIVNAVSEAEKMAGFSINSCLVGVSGDHIRSLNTRGMMAIS
                     RDSRTGLGEAREIEIEDIERVIEHTKAVPLPLDRQILHVLPQEYIIDEQPGVKNPLNL
                     SGRRLDAKVHLTTYNTTVLSNLSKCIKNADLEIDGFVLSSLAAAYACLEESEKTMGSV
                     LLDIGSGVIDIIVFYDDGVHHTGVVNLGANSVTNDIAYMLRVPLEKAESIKREYGFAK
                     VSLTDREAFFRLDSLGGRSPREISIFNLAEWIEPRMEEILRESYLEAKKADIPLTNTL
                     SVVLTGGGARLKGTDELAEMIFNTPARVAAPRGFQEYVTELSDPIYSVAIGLIKYAIA
                     GQISGSALNKTISGPFHRIRGWIKHVTENVM"
     gene            42751..43950
                     /gene="ftsZ"
                     /locus_tag="PLI55_01140"
     CDS             42751..43950
                     /gene="ftsZ"
                     /locus_tag="PLI55_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPA99476.1"
                     /translation="MLFEFEELANQQARLKVVGIGGGGGNAITRMIENGLVGVDFIAL
                     NTDVQALECCKAPTKFQIGRALTRGLGAGAIDDIGRKAAEADRQTIASMIEDTDMVFI
                     TAGMGGGTGTGAAPIVAQIAREQGSLTVGIVTKPFNFEGAKRMARAEEGINQLRKCVD
                     TLIVIPNQKLLAIVDHDTSILDVFKTADSVLYQAIRGISDLINKNGVINLDFADVRTI
                     MKDMGKAIMGTGLATGEERAVLAAQQAISSPLLDEVNIKDAKGLLINITGPANLDIHE
                     VDEACSIITDEVGANAETIFGMVVDENYTDEIMITVIATGFGEAPTPINLSQNKKDFS
                     SPIKDEPRNTDDIWNRINEPGQPVSLFSDDQVIKILNPDDMGMADNPSHKIHQTNIPT
                     YIRQKIK"
     gene            complement(44036..44230)
                     /gene="rpmB"
                     /locus_tag="PLI55_01145"
     CDS             complement(44036..44230)
                     /gene="rpmB"
                     /locus_tag="PLI55_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPA99477.1"
                     /translation="MSKVCDICGKKPIHGNKVSHANNRTHRIWEPNLRKIRIVDNSGS
                     IKTVKVCLTCLKSGTIQKAV"
     gene            complement(44475..47015)
                     /locus_tag="PLI55_01150"
     CDS             complement(44475..47015)
                     /locus_tag="PLI55_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPA99478.1"
                     /translation="MKRSLLFLVTFCLIAASVAWAQSVGDYRSAVNGGKWSVSSTWEK
                     FDGTNWVVASAAPGKDNNVTIRNGYDVILDASGKNCKNLYIEAGATFKADKPNPTSDQ
                     RYVRINGDSAVVSGTFGDPNSPGNNICLENARSGGKIVITGTGTFAPSRFRVNSNVSG
                     DTTIFDIDTKFMYTGSSGSGGVAIYPQTDNNVIIFNAGKTITFVDNANIGVGSSISSA
                     SSQSVKFIVNGTMDLSQPNSSFTLLSAAGDTATLIVGSTGSIVIGDRFLTTGATEGVS
                     RVTIDGSITVNGTATFANENSFVDGTGSFILGDGATLNIGVPAGLDPVAGPIRTSTRT
                     FSTAANYVYNGTVAQVTGPDLPATVKDLVINNTAGVTLSGSAACDTLRLTNGDLALNG
                     KTITWEGATGNGTISETQVLTAPSAVNVGNLGAVITSSADLGSTVIKRGFTADSVNSK
                     LSVSRWYMITPTTNTGLNATLVFNYDESELNGLSEERLLLYSSADTGKTWVERGGIRD
                     VANNKITLTGIDAFSMWTAGSNIPPLPFTMLKPTEGYEVTNSDIVQDSLIEVKWTKAV
                     DPDGNAVTYKLFIFNVDRNELATSTESADTSVLIDAPNYKDNGNYQAYVLAYDTRENF
                     TSSDTVNFILKMNTPPLPFTMLKPTEGYEVTNSDIVQDSLIEVKWSRAVDPDNEALTY
                     KLFILNTDKDSVVISVETPDTSILIDAPTYKDNGNYQAYVLAYDTWEAFSSSDTVDFT
                     INLTPEGIKGDLVPKVLALHQNYPNPFNPITTIKYDLPEEVHVKLVIYDIMGREIRTL
                     VNERQQAGYKSVIWDARNNHGMQVSSGYYICVMQAGDSRKNHKMVLMK"
BASE COUNT        13764 a         9762 c         9385 g        14071 t
ORIGIN      
        1 gtattacctt cttactataa tgaaggtgtc ccaaaatcgt taatagaagc attatcaatt
       61 ggtcgtccct taattacgac tgatcggcct ggttgtagag aagttatcaa tgatgggaaa
      121 aatggaataa ttatacaacc caaaaatgtt gaacaattag taaacgcaat tgaatatatg
      181 attcaaaatc ctgatgagag gataagaatg ggaatagaat caaggttata cgcagaaatt
      241 aaatttgatg taaataaagt aaatgaaata ttttttaagt atatgaatat ttagttcacg
      301 tctaaacctg tatataacag tattatgaga atatgcaatt acaattaatg ttaaaattgt
      361 cgaccaaata tatagagaag agaatgaatt ttattattaa caataacttg atatgaataa
      421 tggattttta tgataaacaa tcccgatata cggttaaaaa tgggaaaaat gtctaggctg
      481 attgctcaaa ataaatttga tgtccaacaa attaataaag agtatttatc ttatctggat
      541 caactatggt agtaaattat tacataaata ttatcacttt ttaaagcgat ataaaaggga
      601 tggttttggt atgtatgttc ggatactcaa tgaagatgag aatatcggat acaataattt
      661 tatagccgaa tcccgtttct caagtatata tcaatctttt gaatggggaa ttgtcaaaca
      721 atacgaccat tgggaggtta ttcgactaat tgttattgat tcaggccaga tagttggggg
      781 actgtcttgt ttatgcaaga aatcgccatt ctttgctaaa cgaatctttt atattcctcg
      841 tgggccggta ttggattatt ttgcggaaaa cgcacctgat attttcaaat tacttttttt
      901 gaaaatacgg gaaattgcac gccgtgaggg ggctttgtat atcagaataa gtcccgatat
      961 tagtaaaaat attcatttta acgattatat ttcaaaatat aatttacacc gcgtccgtta
     1021 cccgatttta cataccacga cttatcgcct cgatcttaca aaatctgaag aacaattgct
     1081 gaactcgatg gaaagtcggg taaaatatga cataaggaaa gctcagcgtg aaaatgtaga
     1141 aattttatct gatgacggta atgaagaata tttaaaagac ttctataatt tgttatatca
     1201 ggtagccgaa cgaaataaat ttccgattta ctcgtatgaa atgatgcaaa atatctggaa
     1261 tattttgtct cctaatggaa tgtgtaaaat ttttctagct agaagccaag gcaaaataat
     1321 ctccggcgct tttttatttc ttttcggtga taaatgtatt tatcaatggg gtggttcagt
     1381 aagggccaaa aataatccca atcaattatt acattgggaa ataattcggt gggcgaaatc
     1441 aaacggattt agcatatatg atcttcaggg tattccagaa aatgtcaaaa aaggcgatga
     1501 attatggggc atatatttat tcaagcgcgg atttggtggc gatcggattc agttaattgg
     1561 agaatatgat tatattttat ctccgatttt atacaagatt tggcaagtaa ttgaaccaaa
     1621 atatccagcc gtgaaaaatt gtttatcaaa aataaagaag aggaaatgag taagagatta
     1681 attttagcct atcatcggat tcttcctgat gaattagcac tcctaagaca gcctttggca
     1741 gtaagtacca cacaatttga gagacaaatt ctctatttaa taaataaagg atggcgaagt
     1801 ctaaccttat cagaatattt tgaaatatat tatttagaac aaaagaaacc tgatagagtt
     1861 tttgtagtca cttttgatga tgggtaccgt gacaattatt attatgcatt tccaatttta
     1921 aaaaaataca atcttaaagc gactattttt ttagtgactg gactattaaa cgagcctaaa
     1981 actttgcaat ctgataataa tattaataac aatttaaccg agatggatta ttcgattaca
     2041 ctagaacaaa tgagagaaat ggctaattat ggaattgaat ttggttcgca tactgtgacc
     2101 cacccgagat tggatacgat cgggttggcg gaggctgaag aggaaattta tcaatcaaag
     2161 aggatgttgg agcaatatta taaccgacag gtgaaaactt tttgttatcc ttatggcgca
     2221 tttaacgacg atgttatttc attagttgcc aaaagtggtt accaatgtgc cgtcgttact
     2281 ccgactcgtc cagggattaa agaaactcga tttaccttga ttcgggttgg tgtctataat
     2341 tctaactcac tcttgaaatt caaattcaaa tgttcaaacc tatttacaaa cattagaaaa
     2401 gcaagattat ggtttctgtt aaagcatttg ctaacttaac ttaggtataa aagataaaga
     2461 tcgtttaata gtattttttg tccggtataa tcttataatc aagttaatga attgtcttga
     2521 taaaattttt caacaataat caaaaaatta cttgacctca tcaaaaaata cttgtagatt
     2581 taatcacgat gagtaaagaa gaggaaaaag actaaatcaa cttggtaccg gcctcgattc
     2641 tttaaataac cctaaaattt atcaacttag aaaaactgta aatcttatgt tttgagtaga
     2701 aatttaatat tcatttagta atcttggctg gaggaatgta gtggttttag catctttatt
     2761 agcagtcttt ttgactactt atatatgcgc gttaggattg acttatctag cacgcaagat
     2821 agctctccgt tatcatattg gcgatatgcc cagtgaacgg aaagttcata aagtgtttac
     2881 gccttattta ggtggtgcgg caattctcct gtcaatcata attggtatcg tattatcaat
     2941 ctatttatta cctatggtcg ctgaagaggt cccgcggcga tattatttcc taggtataac
     3001 cggtgtgcta atcgccctgg taggattgta cgacgatatt aaaaagttac gtttttctac
     3061 caaattttat ttccaaattc tcgcggctat catcgtggtt ttaggtggct taagatttga
     3121 atctttatat attccgatta ttggtgaggt tgggttaggt tggttttcga taatctttac
     3181 gatattatgg attgtattta taaccaatgc gatgaatttg ctggatggat tggatggact
     3241 agcggctgga gtttctacga ttatttttct ggcttttgca ttagtagcat tacattccgg
     3301 tcgaacattg gtagccttag tttgtctatt atttattgca gctaacttag gatttttaaa
     3361 attcaattct catccggctt caatctttat gggtgatact ggttcgcttt ttattggttt
     3421 ttcaacggca gttatttcat tagaagtagg ccgaattggt cacactaatt gtctggatat
     3481 tattattccg cttacgattt tagctgtacc gatattggat actacaattt cctttttccg
     3541 acgaatcaat aagcatatga acccatttat ggcggataag gaacatattc atcatcgttt
     3601 aatgtcaatt ggattttcgc ataaaaatgc ggtcaaggta atgtattttt taagtattat
     3661 tgccggattt atcggctgtt ctttcattta ccttgataat cgnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nntttttagc ggtttattat tggcagattg ttcccaatct
     3841 tttaccggag caattgttga ctccctataa tttaatttta tggatcgcct ggtcagttat
     3901 ctattggact tcgttattgg gcttgaataa tctttaccga attgaatggg atacttcgcg
     3961 agttgatgaa atatttactg tttttaaaat aatatttttc ggcacgattg tattattctt
     4021 tctgagtttt gaattcaaaa ttccctttct tccttcgagg cgaatattag tcacttatgg
     4081 tatttttctg gttactggta ttagtgttgg tcgtttagta ttaattacta ttttgaaaaa
     4141 atacaatttg ttagaattta aaaaacgtcc gacattaatt atcggcgctt cacgtcgtgc
     4201 gctcaatgta gttcggaaaa taaattctgt ccctgagtta aaatttaaca tagttggaat
     4261 ggttgatgat cacgccaatg gaaatatcgg gaaaataatt gaaggggtac cgattattgg
     4321 tagttttgaa aacattcccg agattattaa aaaaagaaaa attaaagaag tcattattgc
     4381 ccttgatgac aataatatga atcaggttat tgatatgatt gatctgtttt caaggtacaa
     4441 cgtttcgatc aaattactgc ctgattttta caatctactc accggttttc gaaccagtca
     4501 catttatgga gtctcattgg taaagttttt cggctctaat atgaaaacct gggaatgggt
     4561 attgaaacgg ctcattgata tcattatttc gttagtagtt ttaatcggat ttctacctat
     4621 ctggctaatc ataagtttga taattaaact cgactctccg ggaccggtat tttataaaca
     4681 aaaaagaatt ggcaaaaatc gtcgcgaatt tgaagtaatt aaattccgtt cgatggttca
     4741 gggtgccgag caggaaaccg gaccgaaatg ggctgaaaaa aatgatccgc gagtcacccg
     4801 tttcggtaaa tttttgcgaa aaactgggct cgatgagatt ccgcaatttt tcaatgtcct
     4861 aatgggtgat atgagcattg tgggaccgcg tcccgagcgg gattattttg ttaatgagtt
     4921 agaacgttcc attcaatttt actcgcgtcg cttaatcatc aaacccggaa tcaccggctg
     4981 ggcgcagatt aaatataaat acgatgaaac cattgatgat gtacgcgaaa aattgcgcta
     5041 tgatttatat tacatcgaaa atatgtcagt tattttagac ttaaaaatta tcattcagac
     5101 tctattaatt ggactacggc gtaaacaccg caccagctac gtctgaaaat tagcgactgg
     5161 aaataacgat tagattataa tatactctat tatcattcca aaattttttc tattttataa
     5221 ggttgaaatg gagaatgaat gttagacatt aaacaaatta gaaacgaacc ggaaaaattt
     5281 cggcgaggat tggaagccaa aaatgccggc gataaattgg atgagctgtt aaaatgggat
     5341 ggcgaacgcc gaaaatgcat taaccaagct gatgaattga aggctatccg caatcgggtc
     5401 agtgacgaga ttggccaatt gaaaaaaaaa gggttaccag tcgcagaaaa gatcctggaa
     5461 atgcgctcgg taggcgatca aatcaataac cttgaccata aattgaaaga aattgatgaa
     5521 aagataaata gtatcctaat tactttacct aatctgccgc acccgagcgt taaaatcgga
     5581 aaatcagccg ctgataatgt tactataaaa gaatgggggc acaaaccgga gtttgatttt
     5641 cccttaaaag atcatttaga tttaggagaa gcactgggaa ttcttgatat gaaaagagcc
     5701 gccaaaattt caggctcggg atttcctatg tatatcggag atggtgccac tctcgagcgc
     5761 gccttagtca attacatgct ggattttcat cgaaatcatc acggatatca ggaagtcttt
     5821 ccgccattta tggttaaccg tgaatcagcc ttcggtaccg gtcagttacc taaactcgaa
     5881 gaagatatgt atcacattga gattgaggac ctttttctaa tcccgacagc ggaggtaccg
     5941 gtaacgaata ttcatcgtga tgaaatactt ccagaaaacc aactgccgat taaatataca
     6001 gcttactctg cctgttttcg acgggaagcc ggttcgtatg gcaaagatac ccgcggttta
     6061 tcacgcgtgc atcaatttaa taaagtggag ttggtttggt tcaccacgcc tgagaactcc
     6121 taccaggtgc acgaacagat actgcgggac gccgaagata ttttacaggc tttgaatctc
     6181 cattatagag tggtggaact ttgttcggct gatttgagtt ttgcggcggc taaatgttac
     6241 gacatcgaag tttgggcacc ggcaagcgga aaatatttcg aagtctcaag tgtcagtaat
     6301 tttgaagatt ttcaggctcg ccgggcaaat atccgttacc gccgaaccag cgataataag
     6361 gtaaatttcg tgcatactct aaatggttcc ggtgtagcca ctccccgact tatgattgca
     6421 ttactcgaaa cctgccaaac gccggaaggc acaatcataa ttccgcaggt tttacagaaa
     6481 tatactggat ttaatcagat aaggaccaaa aaatgacagg tgaaaaaaaa tatagaaaac
     6541 ttatttgcga aatcggcaag aaaatctatg ccaatggtta tgttgcggct aatgacggca
     6601 atctcagcat gcgtttgaat gcggaaatgg ttttaattac accgactaat gtatccaaag
     6661 gtgcggtgaa accttctcaa atagctaaaa tagatttatc tggaaatcca ttagataaaa
     6721 atcgtccgtc gtcggagtca ctcctgcatt tggcgattta taaacgatgt gattgggtca
     6781 aggcagtcat tcattcgcat ccgccatatt cgactgcctg ggcagtaacc ggacgaggat
     6841 tgacggaccc aatcttgcct gaaactatcg tatcaatcgg acaaatccca ttaatcccgt
     6901 ataaaccgcc ttcaactcaa gaattggcag atatagtagc cgatgcggct cagaaaaacg
     6961 atgtgctatt actccaaaat catggattag taacagtcgg caaggattta ttggatgcct
     7021 attacaagac cgaacgcgcc gagcatgtct ttcgaattgt ggcgctggct aaacaaatag
     7081 gagaaattag aaccctgagc aaagcagagg ttgcagaatt gtatcgttta tttaacgtat
     7141 ccgaaaaact acaagtaact tatcgatagt tcttatatga ttaggaattt ctttgctgta
     7201 atctgggtag ttttagttac gattgtattt ggtagcctga gtatggcttt gccgggtagc
     7261 cacaatacaa tgatgcgcaa atggtcgcag atgattctcc aatttcttgg agtcaaatta
     7321 caggttcatg gccttgaaaa tatagtaaat cgaggttcaa aaatcgtaat tagcaaccat
     7381 gaaagtgctc tggatattcc gatcttgatg gcggctttac cagttcctat tcgttttatg
     7441 gcaaaaaagg aattatttca gattcctctt tttggatgga cacttaatct ggcgcgtcat
     7501 ataccagttg accgtaaaag acctcgcaaa tcatatacgg taattaaccg tgttgctaaa
     7561 gaattaatcc ggaacgggct cagtattgct gtcagtccag agggcactcg cagttacgat
     7621 ggtaaaattg caccttttaa aaagggcgct ttccggctgg ctcaaattca taacttgccg
     7681 attcttccgg tattgattct cggagctcgg cattgtgttc cgaataagag cctttcgatt
     7741 attcccggaa tagtcgaagt gcatattggg aaagaagtcc atttgagtga ttatccgaca
     7801 atgaatgatt gcgtggctga tttacgtaac caaatgattg tccaaaagga acaatacgaa
     7861 gaaaaaagaa agcaaggtag ccttgattaa ctgcatccca aaccggattg ccgaaccaga
     7921 aatatccatt accaaaaagg aaaaatccct ttacaaagcc tggttgaatc aggaattcaa
     7981 tctcgcggat ttgcaaacgg ttgaaggcta ctcgctcagt atattatcac ccgggaatcg
     8041 aaacgaaact gaaggtccgg attttcacga tgctttgatt atgttaaatg aaaagttcga
     8101 gcgaggtgac gttgaattac atctcgaatc gaatgactgg tacagccatg gacacgaccg
     8161 ggaccctcat tacaataatg tgattttaca cgtggtttta aactcaggta aattactacc
     8221 gataaaatct tgtgacaacc gtattattcc cacattgatc atgacgccat ttgcgatctc
     8281 agcacctgat gaatatgctt gcttaagctg gaaacatcta tctaacgacg atctcaacca
     8341 agttctaaaa acattcgccg aaattcgctt caaacgtaaa gcccaaatat ggcgctcact
     8401 gattttaaca aaaggcgctg agcagacatt atatgaagga ctggctgatg tgctgggata
     8461 cagtcggaat cggaaaatgt ttggaatact aagccaaaaa gtgacccttg aattgatacg
     8521 gaaaatttta cgagatactg atcccgaaca gcgcattata gtattggaaa gtcttctatt
     8581 tggttcggct ggatttctga ccaaacctga attaaaagta ttcatcagag atacaaaata
     8641 ttcagaaggt ttatataaaa tctgggtgaa aatatcgcaa aaatacgaac tggaagaatt
     8701 ggaatcaacg gactggcatt tcgccggatc gcgaccgccg aattttccga ctttgcggat
     8761 tgcggcactg gcccagataa ttaataaatt ttatcctaac tatctagctg aaacttggtt
     8821 aagagtaatt caaaatgcca aaaattttac tgatattcga cgctatgcaa gtgaaatgtt
     8881 tcaacaaccg gacggactct ggcgcaatca tccattattg aaacaacagc cgggaaaaat
     8941 gctgatcggc gttcagcgct ttaatgattt gttgagtaat ctgctattgc cattcagctg
     9001 ggcgatagcg tccctcgaca atcagtatga attgattgaa aaaaccacta ctttcgcgga
     9061 gcaggttgag tgccttgaag tgccgggatc agttaaggtt gctctgaacc gattgccact
     9121 ggatactatc actttgcacc aaaattattt aactcaggga ataattgagt tctcacgtcg
     9181 gtattgtaac atgaatattt gtaaattatg tccgttagaa aaatatgccc aaaaatgata
     9241 aaataatcac gcgtgaacaa gccggccgaa tcgttagtca actcaaaaga gaaggccgaa
     9301 aggttgcctt caccaacggc tgctttgatc ttcttcacat tggccatctt gaactattgg
     9361 agcatgctag gaaattaggt gatattctga tagtcggttt aaattcagat gattcggtac
     9421 gacgcttaaa aggtcctcag cgcccaatta acactcaatt agatcgtgct cggattcttg
     9481 ccgctctgga agtggtagat tatgtagtaa tatttaatga agatacccct ttagagttaa
     9541 ttcaaaccat taagcctgat tttctgatca aaggtggtga ttatagcccg gatactattg
     9601 ttggtagtgc attcgtcgaa tcctatggcg gaaaggtaat tgtatttccc ctggttcatg
     9661 gcaaatcaac ttccgacctg ataaatacca tatttcaact ttaatcaata aatgctcttg
     9721 acattaagtt ttgtttgaaa taatttccca cccaaaaata tatagaatgg agtgaaatga
     9781 ataatagaaa gaaattgaga ttaatcttat tattttggat gacgataaca attagtgtag
     9841 cattatctgc taataattcc tatgaaaaaa taaataacaa caatggagaa gagaaagaac
     9901 tatcagataa ttctgggaat gatatttttg aacttatttt caatgaagct aagttattct
     9961 atgtagatgc tttagtatct tcttattata atgacacagc cgccgtaaaa tactgttttg
    10021 atcgcatatt tgaaattatc gccgaaatca gtgaacttga tagcctgact ctggttgaac
    10081 aagatgactt caaccgtttt aatgagatgg tatcaaatga ctatcaaacc catttttcgt
    10141 acttgataga agaaactgac tcagtgatga tagtttcggt aggagaggaa ttatttgtaa
    10201 caccattaga tacggtcgat ataggcaatg ataccttaat cgtggtcgaa gaccgaccag
    10261 gacatattcc aatcgttcga tccaaaaaaa tcgataaact tataatatat tattccggtc
    10321 agcaagcccc cgtaattcaa agatatcttg aagcattcaa taaatataaa gatcattatc
    10381 tcccgattct aaaaaaatat aacgtaccgg aagaaattat ttatttacca attttagaaa
    10441 gcggctataa tcctaatgcc tattcttatg cccatgccgc cggaatatgg caatttatcg
    10501 ctggtactgg cgccaaatat ggtctaaagc gtaactggta tgtagacgag cgtcgtgatc
    10561 cgattaagtc tgcccatgcg gcagctaaat atcttagaaa gctttatgaa gaatttgacg
    10621 actggtattt agctttagcg gcttataatt gcggtgaagg taatgtctgg cgtgctatca
    10681 gacgcgaagg cacccgtgac tacttcaaat tacgctcgtt accctcccaa actcgcaatt
    10741 acgtaccatc ttttatggct atcacaatca ttgccaaaaa tccggaaaag tatggactta
    10801 ccacacccac cacacccact tgggaatggg aagaagtgat aatagatcgt tgctatgaac
    10861 tcgatgtcat tgccaaagct tgtgaaacta cacccgacat cctgcggcaa tataatcccg
    10921 aactgcgacg ctggatgacc ccagctaatg ataatcaata tgtactgcgg gtacccaaag
    10981 gtaaagccga gggtttaaca gaaaaattgg tatcactacc agaaccaaag gacgtaaaac
    11041 ccgaggtggt ttatcataaa gttcggaaag gtgaaaactt gagttcaatt gctcgtaaat
    11101 atggcacctc ggtttcggct ttagtttctg ccaatcacct tcgtaatcgc aaccaattac
    11161 gtgtgggtca agtgctcctg ataccgactg gctcctatta ttcggcacct gaaaaaaaat
    11221 cgtcccaaac taatgtagta acccatgtcg tccaacgagg tgaaacatta agtgaaattg
    11281 ctgaaaagta taataccagt gtaagtaaaa taagatcaat gaataatatt cataatagcc
    11341 atatacaggt tggtcaaaaa ctcaaggtac ctactaatct agccagccag atagaacctt
    11401 ccgacaatcc caagggcagt actaaagtaa tccacgtcgt taaaaaagga gatactttaa
    11461 cttcaatcgc caataaatac aatgttagtc ttacaaaatt gaaatcgtgg aacgatctgg
    11521 atggtcgcaa accaatttat cccggtcagc ggatcgtaat ttaccagtca attaaaagct
    11581 aagtcaaacg gttttccctt tctgaaagtc tgaaatgttg tgtcatataa atcgtacttg
    11641 gtcaaaatcc tctactatta tgacaaaagg ataagcctaa ttatagaaaa ataatggctt
    11701 gcctttatta tgaattctat aaacatctct ttgatgtcta atgtcggtca tttaattttg
    11761 actatggtat cttctgaaat ccttaatacc gaaagacacc gcgacggggt aaaatatttc
    11821 tgcaagttat tccgtatcga atcttcacta ccttccataa accacgtcac cagtattctt
    11881 cgcaaatttt cacttcttcc ctatgaaaat atcagcaaaa tccttagtct gaaccaaaat
    11941 tgggacgaaa atcatttccg ctggcctcag gaaatcatct gcgactatga aaaatataaa
    12001 ttaggcggta cctgtttctc tcttactttt tttctaaagg caattctcga ttattttggg
    12061 tatcaaacca caatattaat ggccgatatg aaagccggac gaaatactca ctgcgcactc
    12121 ttgcttgaat tcaaaaaccg caaatatcta cttgatcccg gctatttact ccacttccct
    12181 ttaccattaa actcggccgt catttctgag aaagtccaat tggaatatga tccattttgt
    12241 gagaggtatt cactctcgac attagaaaat ggcaagaaaa aatggcggta ttcatttcag
    12301 gaaagaccga cttctatcga ggattttagt cagtactggg aagattcctt tcattggatg
    12361 actatgcacg gaatctgcct gtccaagcgc gacgaaaata atttcattta cctgcataat
    12421 cattatttaa aattggaaaa cagatcccag cattttaaag gaaatttgaa tacggaaatt
    12481 gcagaactgg ctcagcgttg gtttaatatt ccaccggaaa ttgttcggca agccgaaaaa
    12541 gctttgcgtg aaaatttata ccaagataaa atattaggtt atcaaatacc aaaatggttt
    12601 aaatagcaat tgctaaaaca cttgaaaaaa taaaatatat taaaaatctt gcttaataac
    12661 cacttagaaa acagattgtg gttaaaatat cagactagac taactcttaa ttttttagtt
    12721 tgagaaaaat actggggtat ttttatccca aataacctct taaatgtaaa gccctactat
    12781 tatgccgtaa ttttcgtaag gctttttctt taatctggcg aacccgttct cgcgttaaat
    12841 gtaaacgttc ggcaatatct tccagattta acggtagatc gctgtcaata ccaaaataaa
    12901 gacgcaggat gtaggcttcc cttttgtcta aagaatttaa tgctcgctcg atctctaagt
    12961 gaaatgactc ctggtttaga gtaagtccag gatcattatc ttcctggcta ggtagaatgt
    13021 cttgtaacgt accggtttga tcgttgccga ctggagcatc aatactgata gtattttcag
    13081 ttaattggcg ggcagaatct atgtcaatat tttcattttg caacaacact tcaatttcat
    13141 cgttggtcgg ctctcgttca taatcccgtt caaattctga agtaattttg gtaatcttgt
    13201 taagattacc gactacgtta agcggtaagc gaaccagacg tgaattttct gagatagcct
    13261 gcaagatagt ttgccttatc caccataccg cgtaggagat gaatttaaat ccacgagttt
    13321 catcaaaacg gcgcgccgct ttgattaaac cgatattacc ttcattaatc aaatcagcga
    13381 gagaaagacc ataaccctgg tattttttag caactgatac tacaaaccga agattagctt
    13441 ttagaagccg atcaagagcc tgggtatcac cggctttgac ttttctggct agttcaattt
    13501 cctcatccgg tgtgagtgtc ttttcagtcc ggatttcatc aaaataacgg cgtaaaacct
    13561 cgttggagat gaatggtgat tttctagatg aagatgaata attagtcatg gccatttccg
    13621 gccaggttaa taatggttaa gtttctgtct cacttttaga ttgatccggc tcatccggtt
    13681 tagcagtatt ttctttagaa ctttcctgaa tcttgtaata ctgggcttct ttatctttta
    13741 actttttctc gagttcttta atgttattaa tcaaactggt taccttttca tttttaacga
    13801 gattgccttt agtattttga ttcagcaatc cataaactac tcgacctaat tcggtgtaat
    13861 tcttatcaat atccctcttg atttgaaaaa tatccagttt aattttgccc agctgggtca
    13921 tttcctcggc tttctcggcg gcttctcgag ataattcacc ggctttttca gcggctttag
    13981 atgcaaatcc tccgacactt tttttaatat catcccaaat accagtcata ttgtcctcct
    14041 aatttttttt cttactattt aaaaaatcca cctaaaatta tttataataa aacataatgt
    14101 ccacaaaaaa taagagcctc ctacaattag gaggctcttt tatggacaat gtctttaatt
    14161 tttaatacat gtcgcccatt ccacctggcg gcattgccgg tgcggctggt tctttttccg
    14221 gcacctcggt tattacagct tcagtagtca acatcaatcc cgcaatactt gcggcatttt
    14281 ggagagcgat gcgggttact ttagttggat ctataattcc ggctttatac atatgaacat
    14341 attcttcttt atatgcatca aatccgaaat catctttccc ttctttaatt ttctgggcaa
    14401 cgatagatgg ttcatgaccg gcatttttgg ctatctggcg caaaggctct tcgagagcac
    14461 gtttgataat ttttacgcca accatttgat cgccagtaac tttaatttta tccagagcag
    14521 gaatgcatct gattaaagca acgccaccac cagggactat accttcttcc actgcggctc
    14581 gagttgcatg cagggcatct tctaccaaag ccttgagttc cttcatttca acttccgtcg
    14641 cggcaccgac tttcaggaca gctactccgc ccgagagttt agctaagcgt tcctgaagtt
    14701 tttctttatc ataatccgat gtactggctt caatttgagc tttaatttgt ttgattcggc
    14761 ctttgatatc ctcgggttta ccggcacctt cgacaatcgt agtattgtct ttatctacca
    14821 cgattctctt cgcatttccc agatgtgaga tagtagcatt ttccaatttg taaccggctt
    14881 cttcagatat aactttacca cccgttaata ctgcaatatc ttctaacatt tctttacgac
    14941 gatcgccaaa tcccggggct ttcaccgcgg caacttttaa agtgcctctt agcttattga
    15001 caattaaagt ggctaaagct tcgccttcta catcctctgc aattatcagt aaggaacgcc
    15061 ccatctgcga cactttttcc aaaatcggca acaattcctt catattgctg atttttttgt
    15121 cataaatcaa caaataagga tcttctaaaa ctgcttccat agcctcggca ttcgttacaa
    15181 aataggctga aatatatccg cgatcaaatt gcataccttc gaccaagtcc agagttgtca
    15241 gagtactttt ggcttcttca acggtaataa cgccttcctt accgactttt tccattgcct
    15301 cggcaattaa tactccaatc ggtatttcga ctatatcctc tttacctgat tcatctttta
    15361 tcatccggcg gactttccgg ttattagctg agatagttcc tacctgagct atttcttctt
    15421 tactgtttgt cacagctttg ctcaattctt ttaggctttc gactaccgta tttacagccg
    15481 tgtcaatacc acgtttcaac tccatcggat cggcacccgc ggtaacgttc ttgatgcctt
    15541 cagcaataat tgactgggct aatacagtag ctgtggtggt accgtcacca gcaacatctg
    15601 aagtttttga tgcaacttct ttcaccatct gagcgccgat gttttcaagt ttatcttcca
    15661 gttcgacctc tttggctacg gtaacaccgt ctttggtaat cgtcggagaa ccaaatttct
    15721 tttcgataac cacattacgt ccttttggac caagggtaac tttcacaaca ttagccagtt
    15781 tatcaacgcc ctcttttaga gcagttctgg cttgtatttc ataagtgatg tcttttgcca
    15841 tatttgactc cttttgttag cattaaagta ttgcaaaaat gtcgctttcg cgcattatca
    15901 aatgttcttc accatcaatc attacttctg taccagaata ttttccatac aaaacatgat
    15961 cgccgacttt tacttccagt ggaatttttt tgccatcatc agttgttttt ccagggccaa
    16021 cagctattac tgtgccttcc tgtggttttt ctttggcagt atcaggcaag tatatgccac
    16081 ctggagtttt ctcattagaa actaggggct taactaagac gcgatctgct aatggtttga
    16141 ttgccataga atttctcctt tcaattaatt tattaattat tgattaacca attgttttta
    16201 gccattattc tccttcaagc attcaaagag cgcatttaaa tttacaaatg acgtgccagt
    16261 tttcggctcg gacagaaagt cagagaaaaa tatttcagct gactgtaacc atagatagat
    16321 tatttctagt aaaagtcctt ttaatgtaaa ttttgtttta attctttcgt attttcctat
    16381 gacgattatg atcctgatat gataagttca aatttccttt ttattctggt gggatttgtg
    16441 gctggtgttc tttccggctt gtttggtata ggaggtggta ttgtaattgt tcccattatg
    16501 atttggttct gtcatttttc tcaattagcc gcaaacagca tttcacttac ggctttactt
    16561 ctaccggttg ggattctggc cgttttggct taccataaat ccaagttggt taaagtacgc
    16621 caggctttat ttataagttt gggaattttt atcggcgtgg ttggcggcgc tattttagct
    16681 cttgagattc ccgctcattt cctgaaacga ctctatggca ttttcctttt atatattgct
    16741 tggacttata ttgacattcc atcattattt gcaaatggag ctaaccaaga gaaaactaac
    16801 cagcaatttg aaaatttaaa tttggatcga ccggtttgtt atatagtgct gggatttgcg
    16861 gcgggaataa tgtccggctt atttggcatc ggtggcggta ttataatcgt accggccttg
    16921 atctcaattt ttaaatatcc atataaaaaa gctgtcggta catcattgat ggctttactt
    16981 ttaccagtag gactgccagg ggtgttgatt tataatcgcg ccggacaatt acaaatcggt
    17041 ccagcgctat ttattgctct cggattatta tttggtgcgt tagttggagc tttagttgca
    17101 atcgagttgc ctactaaact aattaagaga atctacgggg gatttttatt gttagcaggc
    17161 tttaatttta ttttttaata aaatcaagac gataccgcta aagcggcttc taatcacggt
    17221 ttaacgacgg ttactttcct cgattttaga agctttgccc cgcaaacctc tcaggtaata
    17281 taacttagcc cgacgaactt tgccgacgct gattcttttt attttcgata ttttcggaga
    17341 gtggagtgga aaaatccttt ctactcctat tccattggag atttttctga cggtgaaagt
    17401 ctcactaatt ccactgccgt gacgagcaat gacgactccc tggaaagtct gcagacgttc
    17461 tttatcgcct tcaataatct tgacttcaac cgcgacggta tcacctggtt tgaattcagg
    17521 aatatcggtc ctaaactgat ccgcaactgc ggcggttaat ttatccatta tttttatcct
    17581 ccatattcat atttttaatt aaatcaattc ttctttggcg agttctaagt ttgcgttgtt
    17641 cctggcgcca ggcgtttatt ttggcgtggt tacccgtggt caagacctct ggaacacttt
    17701 tccgtcggta aacctgtgga tgggtatata ttgggccttc cagtagatta tccgtaaagg
    17761 aatcggtctc agccgactca taggaatgga gtacccccgc tatgtgtcgg ataactgcat
    17821 caataataat gagcgccggg agttcaccgc cggtcaacac ataatcaccg atactgattt
    17881 catcagtcac caggttagcg attactcttt cgtcaacacc tttgtagtgg ccgcaaaaaa
    17941 aagtcaagtc tgactcctga gctaattcga gtgctaaatg atggttaaac aatcttccct
    18001 gtggcgttgg aaacacgacg cgcggtcgtt tatgcccagt cagtttgaca attttatcat
    18061 aagcacgaaa aaacggctcc ggctttaaga ccattcccgc accgcctccg tagggtttgt
    18121 cgtcaatctg tttatgttta tcacgggtga aatcccgtaa attccagaca ttaaattcaa
    18181 ccttaccatt ttcgacggct ttgcggaaca tgctttcaga taaacaggct gtaatcattc
    18241 ccgggaatgc ggtgattatg taaattttca tttcggaaat aaaccttcga tatctttaaa
    18301 aataacaatt tgttgttcag ggtcaatctg ttcaattaaa tcggttactg ccggaacaag
    18361 gagagtcgcg tcttccgatt gaatgacaaa taatgcctgc ggattggttt cgtccaatga
    18421 actaataaaa ccgatatctt tggctgaatt gattactcta accctgaatc cgataagctc
    18481 aacgagctct ttcttcaatt catattctcg tggaacgaaa acctttagtc ctctaagata
    18541 atccgcttcc tgccgggaat taatatctcg caatttcaaa taaacttttt tagtacttat
    18601 tctcagataa tcaaccttcc atgggcgagc tacctgagca ctttcaccga gccaaagtac
    18661 ttttggctca tctactttta tatttttcag agagccgcga tttaaaagga ctgccccgtt
    18721 cagaccaaag ctttttaaaa tggatccgac cggtaacagt accaaatgat ccattttaac
    18781 gcttattcaa tgatttctaa aattgccctt ttctgacctt gtttggcggc ggcggcagtc
    18841 aaaagtgttc ttatcgcgcg agcggtgcgc ccttttttcc cgatcacttt tccaagatca
    18901 ccttccttga ctgaaagttc cagaattacg gttttttcac tatcaacctg caccactttt
    18961 acatcttccg gatggtcgac taattgtttt acgatgaatt caacaaactc tttcatagnn
    19021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat tctcgacgac tgggacagat
    19141 tcgggttctg ggacctcgac ggcttgtgct tctttaactt cttcagcgat ttcagcgctt
    19201 ttattatgag ctaactgcca tttttggatt tcgatatttc ttacctgttc gctgatatta
    19261 ttgcgaatca aatgccactc cattgcaata ccttctttct gtaatagatt gaaaactgta
    19321 tctgaaggaa cagcaccttt ttctaaccaa ctaaaaacct tttccttatc tattttaacg
    19381 gttgccggtt tggtcagcgg atcgtaataa cctaatttgt caaggtatct accatcccga
    19441 cgacatttac tgtccattgc cacgatccag taaaacggtt tcttctttct acccattcgt
    19501 tgtaatctaa tttttactgc cactaagagc ctccttttat ttaattacct attccaaaag
    19561 cagataaatt cttgggtagt ttcatccgac ccatttgttt cattaacttt gttatctgcc
    19621 agtattgatt aagtagacgg ttgacatcct gcggctgagt accactcccc agagcaatcc
    19681 gtcgtcgccg actaccatta attatattgg gtttaagccg ttcggttcga gtcattgatt
    19741 gaataatcgc ttcggcttta atcagttctt tttcatctac tttttgattt ccgagtcgca
    19801 tacgggcaaa gccaggcaag tgttccatta cgctatcaag cggtccaagt ttttttaact
    19861 gtcgtagctg agtcaaataa tcttcgaggg taaattgttc tttttttaat ttattttcta
    19921 atttcaacgc cgattcaagg tcaacgctcg cttgcacttt ttcgactaat gagacaacgt
    19981 cacccatccc gagaattcgc ccggctaccc gctccggatg gaaaatttcc agatcgccga
    20041 tcttttcacc gacactcata tatataatcg gtttcccagt agaagaaacg atcgtcaaag
    20101 cagctccacc gcgcgtatcg ctatccattt tggttaatat gattccgtca acaccaattt
    20161 gttcgttgaa agaagtagcg gtgttgactg cgtcctgccc ggtcattccg tcaacaacca
    20221 gaaggatatt atgcggtttg atggtatttt tgatagtttt taattccgcc atcatttttt
    20281 catctatgtg caaacgcccg gcggtatcaa ttattagcgt atcgcgatgt tgctgtcgtg
    20341 caaaagtaat tgcatcttgg attcgcccga ctacatcggg gttctcgcga aaacagggaa
    20401 cttttatctg tcgagcaaga atctctaact gctcttcagc ggcgggacga taaacgtcca
    20461 caggcaccaa taccggacta tgttgcttct gaagtaattg ataagctaat tttgcggcgc
    20521 acgttgtttt tccgctacct tgcaaaccga ccagcataat gatagtcggc ggaatagaag
    20581 cctccttcaa attgcggttt ttatcaccaa gcagatgaac catctcatca tagatgattt
    20641 tcacgattaa ttgtccggga gacaggcttt tggcaaccgt taccccgact gatttctcct
    20701 gtaccgactg aacgaattct tttgcaacct tgtaattgac atcggcttca agtaaaaccc
    20761 gcctaatctg acgcattgca tccgcgatat tattctcagt tatccgccct tctccacgaa
    20821 gggattttat gatcgcacca aaaccgtttt gtaattgttc aaacattgtt taattcaaat
    20881 tcattaaagc gggtaaattt aattttttta acttcttttt gcaagatatt atcctcgcag
    20941 gattatatag tatcaagata tggtatccac cgtttcatca ctaaagaata taaggattta
    21001 tttgaaaata aaggaagatt gagttacctt ttgcttgtga atgctaatta aaactaaagg
    21061 attatcatga atagaagaca tctaatgatc gttttgacgt tcccaatatt cttaatgtat
    21121 aattgctccg aaacacaacc tgagtccagc aatccctacc gaataaattc gggactcaat
    21181 cctggaataa atattggtaa tgctctcgaa gctccgaacg aaggcgaatg gggcgtggtg
    21241 attaaagatg aatatttccc attgataaaa tcggccggat tcaattccgt ccgtttaccg
    21301 attcgctggt cagcccatgc tgatattaat ccaccttata cgatctcgga cgattttatg
    21361 aaccgagtgc gtcacattgt tgacactgct cttgagcaca aattatatgt agttatcaat
    21421 gttcaacatt ttcaggaaat ttacactgaa cccatagaaa ataaaccgcg cttgctggct
    21481 ttctggcatc aaatcggctt agcattcaag gactacccgc aaactttact tttcgagcca
    21541 ctcaacgaac ctcatttcaa tctcaccccc gaattgtgga atgaatggat acctgaatta
    21601 attgatacga ttcgggtcac aaatcccaat cgcactttgg taatcggaac agccgactgg
    21661 ggtggaatct ggaaaatgaa agaattaaag ttacccacta ctgaacgaaa tctaatcctc
    21721 gcaatccact actatgaacc attccggttc actcaccaag gcgccgagtg ggaaaaagaa
    21781 agtaatgcct ggctggggcg cacttggacc aaaaccaccg atcaaatgca agaactcaac
    21841 cagcatttcg atctaattaa aaaatttgct gactcgttag gagtaccggt ttatatcgga
    21901 gaatatggct gttataatcg tgctcccgaa gaatctcgat taatttggac acgaacagtc
    21961 tccgaacaat gtgaaaaata tggatttagt cgtgcctact gggaattctg ttccagtttc
    22021 ggtatttatg atccgatcag tcaacaatgg cgggagccgc ttcgttcagc agtattattg
    22081 gcgaggtgaa aattaccaaa atcattgatc atcaattccg aaaaactttc cccttgaatc
    22141 actctactag attaaaattg tcagataagc ggtaattctc attagaaatt gaagctatga
    22201 cggaatattt cataaccatt atgatataac cggcaggtga ttaatcctgg tgaaaagtaa
    22261 tggaacttaa cttcgtcata atactttaat tttggtcata gctaaaatca gacgtaattg
    22321 catatcagaa aacaccaatg taaattccga caaatttgat ttttatgata attaacaaac
    22381 gattctgcgc taaaataata ttcacttcat tttgtctatt cagtgctatg cttgttcggc
    22441 tcgaggctgt cgaattgaca attttaagcg aaactacttc agagctgatt cttcgggtcg
    22501 aaatggatgc cgacttagaa tggcgccaaa tagaaaatta cccgaataaa gtcagcgcct
    22561 tgactttagg agacgcctac caatcccatt tgagtcaaac cctggaaatt ccttactatc
    22621 aatggctaat tgcacttccg actgaggata aaccttcagt atccataacc aaatctgttt
    22681 ttgagtcagt caaactctta aaaaacctga ctacggacga gttaacggct ctcgataaat
    22741 ttcccttggt tgcgatcgcc gagaccggtt ttttaagaga tgttcccttc ggttcgcttg
    22801 taatatatcc cctgcgttca gcaaataatt ccaatgaatt acgtttattg cgcgagattg
    22861 aaattacaat acagtacaca gcagtaagaa aaacgactat caatcagacc acggtagaaa
    22921 agggcgatta tttaaatgct tttgtgaatg gatcatcagc cacacaatgg cgacaatctc
    22981 gcccacttag tctacggaaa cctcagagcc agcctgccgg caagtggttt cgcctaacgg
    23041 taaaaaagga tggcgtttat accctaaccc tcgacgacct aaaaagcgcc ggattgactg
    23101 agacaagcat agatacggga cgtatttatc tctactctaa ttccaccggt gggcgtcctc
    23161 tggatgcaac aattggcgcc gccgttccat cgaacttaat cgaaaatact cgctggataa
    23221 aaggtaataa cgaccaagat tttactaacg ctgatacgat tcttttttac ggtcgggcta
    23281 caagcggagt tgacctcgat tctaaaaact ccctcacttt cgtgcgaaat ccttactcgc
    23341 taataaatta ttattggtta ttcatcgccg atgagcccgg ctatcctaag gctatgccga
    23401 ctataaatag tctttcagcg acccccaata tgaccgtaac tcaaagtgag catctcgagg
    23461 ttcacgaaga agaaaaggtt aattttttac gctccggatt aaaatggagc ggagaaaaat
    23521 tcaatacctc gggttcaaat gtttcggtaa ttctgactat gccaaaagtt cgtgatagtt
    23581 acgttggtgt aatgacaatc tgtactcgcg gagcggttga tgaaatttta cattatttta
    23641 aagcttattt gaacaatagt acgacgccgg tcgccagttg gtcagccact aattatcaaa
    23701 gtgccactac ttctttttca acgactcttt cagctaacat aaatattttg aaaattaact
    23761 atacttccca acctagtagc gccagcgctt atctggatta tgtaatgttt cgttatacta
    23821 aagacctaag cgcattcact gaaccgcaaa ctttctggtc actgttgtat tctggaatag
    23881 tcgaatatca aattaccgat ctaaatttta ccaatctact gattctggac gttagcgact
    23941 ggagcaatgt ctccatccag caatacaacc gaaattctaa cctaatttca ttccgtacag
    24001 ccagtcaatc caatcagcgt cggcagtatt atcttaccgc tccatctttt tttaataaac
    24061 cggaaaaaat cgagttaatc ccgaatcctc aatggattac attgcgcaat ataaccagtt
    24121 cctacgatta catcattatc actgccgatg atttcctgac cgcggctgag aaaattgcca
    24181 ctctatattc acaggaactt tcccccggag accgtcttaa tacattggtt gtcactcaaa
    24241 atcaaatcct gcgcgaattt aatgccgaca tcaccgatcc tcacgccatt cgcttttttc
    24301 tgaaatatgc ctatgaaaat tggacacccg ctccagcgta tgtactgtta cttggcgacg
    24361 gcacttttga ttaccgcgcc atcgaatcca gcacaggaaa ttatgtaatg acctatcagg
    24421 ttgaaccggc tgtagaagat tactccggtt tttcttccta tgcttccgat gctcgttttg
    24481 tctatattaa aggttcggat caggttatgg atatggcaat cggtcgggtg cctgtgagaa
    24541 ctgctacgga agccctcgat tattatgaaa aattaagaca atacctcact gaaccaattt
    24601 atggcgaatg gcgcagtcgg atcacgctgg tcgccgatga tccgcaacga ccttataaca
    24661 atgaaaaata tcatataaat gatactgaat cttatattgc caaatatatt cctaaaagtc
    24721 tcataaccga taaattgtat ttacttgaat atccagaagt ccaggatgta tccagtaatt
    24781 gggtaaaaaa acctgccgcg accgaagcca tcttaaatca actcaaacgc ggcacaactt
    24841 tgattaatta tctcggccat ggttcttcca cagtttgggc gcaagaagaa gtactaaaaa
    24901 tggaccgcga cattggttta atcgaagccg gaatgaaatt accgctctgg atcgccggaa
    24961 cctgtagctg ggggcaattt gacgacatca atactatttg tatgccggaa gccttaatca
    25021 ataaaaatag cgacggagcc atcgctatcc ttacaccaac tcgaccaact actggtactc
    25081 agaatgccag tttcataggc aatttcatca aaaagttatt tccaagcggc gaaatcagtc
    25141 gaaccagact tggcgctcta attcaaaata tttataacgg cacaaatgaa aatcaggaga
    25201 aatacgttct attcggagac ccagcccttt acctggcgtt accgtatcaa aaagccgctt
    25261 tcgataaaat gccccgcgac actttagcgg cattggaaaa ggtcagtgtt accggacgaa
    25321 cagttaccga agagaatttc aacggtgacg gtattatcaa tgtttttgat agcgatcgct
    25381 acgtaacccg ctattacctc gacgacgcta aagtaaccca gaatcttagt tacactcttt
    25441 ctggtgaaac gctctatcga ggcaatctta aaatcgaaaa tagtaaattt ttctcccgct
    25501 tctacatccc gaaagacctc agttatatga accttttcgg ccggattaag gtttacggct
    25561 ggaacacttc tgctatgagt gattttgccg gttgttatga ttcattatta tttaccggta
    25621 ccacccaatt cgatgatacc agtggcccgc aaatcgtgat cggctttgaa ggtatggatt
    25681 ttaaaagtgg agacatcgtc gctcccgatg ccaaaattaa agtatcaatt atggatccag
    25741 tgggaataaa catcgccggc aaacttggac acgatatcac tttccgtttt gataatgata
    25801 atgaccttat ttttgaaatg acggaagact ttgtttatga tgtaaatagc gactcttcag
    25861 gcgttatcac cagtgacttg cctgatctct caccgggaat ccaccaaatc actgtaaaag
    25921 cctgggataa tgctaataat tatgcaaccg ccacagctga atttatcctt gccagttctg
    25981 ataaattaaa attggaaaga gtggtcaatt tccccaatcc cttcgctagt acaaccgact
    26041 ttgtctttta tcttactcag ccgggacagg taaaaatcac catctacacc atccgcgggc
    26101 taaaaataaa aaccatagcg agcgatgaaa tcctaccacc tggtttccat caagtgaatt
    26161 gggacggatg tgacgatttc ggtgatgaaa tcgcccgcgg catttacctc tataaagttc
    26221 aagtacagtc atccgattca aaccaaaaag ataccttcat cggaaaaatg gtgaaatcat
    26281 gacgacccaa ctcaattggt attctatgcc gattattttg gcttctcaat cgccgcgccg
    26341 gaaagaaatt ttagacatga ttggactgaa atacgaagtc tgtcctcccc attatatcga
    26401 aaacaattca attggcaagt cgccgacaac catcgttata aaacacgccc tgaataaagc
    26461 ccgaaccgta gcacctcgct atccgaaatc ttggattatc ggagccgata ccgtcgtcgt
    26521 ttgtaaaaac agaattttag gaaaaccaca gaatgaaaac gaggcggttc agatgttaaa
    26581 attcctgagc ggcgtcgaac atagcgtctt caccggatat tgcgttctga attccgcaaa
    26641 tggtcgttat ttgaaaaatg cagtgcgaac ccgggtcaga tttaaagaaa ttcctttggc
    26701 ggaaatccaa tattacgtcg aaaattatca tccgctcgat aaagccgggg cttacgccat
    26761 tcaggatttt agcgctattt tcgtggaaaa aattaacggt tgtttttaca atgttgttgg
    26821 ttttccacta ccggctttct atgaacaggt taaaaatgaa ctttctatct gcctctgaaa
    26881 gtttttattg aaattaatag gaatttttct gatatttgca acaatggaaa tgaacttgag
    26941 gaacaatggc aacgttttat gaaatattaa aacaaagcag agaagcacag ggacttaccg
    27001 ttgagcagat ttccagccgg accaaaatca gcaaagaaat aatcacagcc atcgaatccg
    27061 gtgatctaaa aatattgccg aaaacatata tgcgtttatt cctgaaagcc tattctcagg
    27121 aattaaattt agaccctgaa gcaattctta aaaatttcga agaatcgctc ggcgaaccag
    27181 ttgagacacc ggcagttcca gacaacaaca ttaagccgag tttacccatt tcacaaaagc
    27241 cggaaaaacc agaaaaacca ataataggaa aaaaacaaaa ccgcaatttt tctactgtaa
    27301 taatcgtttt tgtaattctc gtttttttga ttgcagtcct cagacaggtt ttgatggatg
    27361 aaaaaaagac gagcagtaat actgcactac cgaaaattac tccaactgac acaactcaaa
    27421 ctattcctcc gagtcccgat ctcgaagtag cagttcccaa aagcgaattg aatttaacta
    27481 ttacaactaa agacagctgc tgggtgcgga ttgtcataga taatcgtacg acttttgaag
    27541 caactctgcc accactctat aaaaaagaaa tgatcgccaa agagcaattc gatatgcaaa
    27601 ttggtcgccc cgcttctatt aatctagttt taaatggaaa agacttaggc ccggttggca
    27661 ttcctggcat tccaacccgt ttaataataa ccaaagatgg gattattagg cgtcagagtt
    27721 tttaatctca ataacttttc ctcattttaa tttctcctct ttttaaataa cccattaatc
    27781 ttcttaaaac cttttaaaaa aactcttgac aattagtttt ttttgtccta ttttgtgggt
    27841 gaatgtggta agaaatccca aataatccca aaaatgcgag gaaactgtaa acccgatgat
    27901 tactgccttt gccggtgaaa cccgccaccc acttgacgaa aaaaatcgct tgagtattcc
    27961 ggctaaatac cggcgttggg ctgatggtca ggacggcgag tgtacctttg tggtaaccaa
    28021 aggctcggat ccttgtttag ttgtctatcc atccaccgaa tgggatgtat tatctgagaa
    28081 acttttgaaa ttatcctatt ttaagaagaa aaatcgggct tttatccgag cgctggcacg
    28141 tagttctgtt cgtctaaagt gtgacggcca ggggcgtatt ttgataccta caagtttact
    28201 tgaatacgcc catattaaaa aagaagtaac gatcatcgga gtattgaatc gtctcgaatt
    28261 atgggatccg gaaacacttg aaaaacatga agagtcttcg attcctctcg atgatgaata
    28321 ttacgaagac ctgggcgaca ttctttaatc ttgatatttg gaaaattatg gtatgactac
    28381 cctccatatt cccgtattgg ttgaacaaac aatccaattt ttgatcaata gaccgacggg
    28441 aatttacgtt gactgtaccc ttggtaccgg tggtcatttc caagcgatag cccaacattt
    28501 agataatgac gcatatctaa ttggtataga tgccgatccg agtgccgtgc aatattgcga
    28561 gcaaaatatt tcgatttctc agcctcacca gtttatcact gccaattttg ctgatattaa
    28621 acggatttgc tatcgggcag gctttccgaa agtcgacggt attttaatgg atctcggatt
    28681 atcatcattt gccttagaca atcccgagcg tggttttact tatagtcagg ctggtcctct
    28741 cgatatgcgc ttctcgcctg atactgctca gactgcaatg gactttatca atacagcctc
    28801 cttgaaagaa ttggtattag ttttcaaaga atatggtgaa gaacgttaca gccagaatat
    28861 tgcccgtcgg attatagctg atagagataa aaatcctatt aaaactactg gccaattagc
    28921 ctctattatt cgagaagaag ttccaaagca atttcaaatt aaaaccttga gtcgtatttt
    28981 tcaggcgatt cggattcaag tcaatcagga attagacgct ttacgacaag gtctatcaga
    29041 tgctgtttca ttattagccc cttccggacg aattgtcgtt atttcttatc attccatcga
    29101 ggatcgcatc gtcaaacaat tttttaaaaa ggaagccagc gattgcatct gccctcctga
    29161 tttcccaatt tgtcaatgta atcaccatgc ccagttaaaa attttgactc atcgcccgat
    29221 agtgccgagc aaatctgaaa tagaaagaaa ctcacgggca cggagtgcca gactgcgtgc
    29281 cgcggaaaaa ttgtagagat caaatatgtc cagccgcaag tcatttcgta aaaagaaaaa
    29341 aaacctactg ttgataattg gctttttcgt tcttgtcggt tcaatggtat tttatatttg
    29401 gatttacaac accactaacg ctctgtatcg cgaaatagac cagttaaaac gaaaggaagc
    29461 cagtctgatt gcccgaaatc agatgctcgt cgtcgaaatt gagagactga gtcgggcaga
    29521 tcgcataaaa aatattgcga caaccgaaat tgaaatggtt acgcctttac cagaaacatt
    29581 agcagttgtc atagattcta aagttttagc agttgaataa tggctaatca atttaaatcc
    29641 attcggaaaa ggactttgat agtgagtagc tcattagtgg ttctctgggt ggttttgata
    29701 gcacgccttt tttatattca agttttaaat tcaaaaaaat atcaggaatt atgtaataat
    29761 caagccaatc tgcgcaaagc cgtgttaccg cgccgaggca tattttacga tcgcaacggc
    29821 gaggctttaa ccattgattt agttaattac tcgatcgcgg cgcatcccta cttggttgaa
    29881 gataaacccc gtttagccag tactctggct aatgatttga gcgccaaacc cgagcagtat
    29941 cttaaactgt tggaatccaa taaaactttc gtctggctgg aacgcgaagt cccctattca
    30001 agttttcaaa attatgaaaa atatgaaaaa actcccggat tggttatcga aaaaaatgtt
    30061 aaacgctact acccactcgg tgaaattacc ggtcaattgc ttggttttac aaatatagat
    30121 aatcgcggag tttgcgggct ggaaagcgga ttggaaggtt tactttgcgg aacgcccggc
    30181 tggattatgg ttcaaaaaga cggctgggga cgacttcact atcggccgga tttgccttct
    30241 aaaagcagta ttgacggcaa tgatattgtc ttgacaattg atcgggagta tcagactatt
    30301 ttatatgagg aacttttaca ggcttacacc gaaacaaaag ctgaaaaagc tatgggcatc
    30361 gtgattaacc ccaatgatgg cgatatctta gctatggtta ccttgccacc tttcaattct
    30421 aataaacctt ccgattatcc gatttctgct caacgcaatg ccgtagtttc ggatatattc
    30481 gagccggggt caattttcaa gattgtaacc gcgacagccg ctctcgataa caaagttctg
    30541 caacctaaac accaaattga ttgtaatccc ggttttattc aagtcgggaa tcggataatt
    30601 cgcgatcatg ataaatacag cattctgact ttcgcggaag tgataaaaaa tagcagtaac
    30661 gtcggaacca ttaaagtcgc ccaattaacc ggaaaagatc aaatctttaa ctatgctcgc
    30721 aagttcgggt ttggggcaaa aaccgatatc cagtttcccg gtgaagaatc cgggatttta
    30781 cacccattta aaaaatggaa cgacctagtt ctggctcagg ttgctatcgg tcaaggagtc
    30841 tgcgtcacgg ctttacaaac cggaatggct tatgcggcta ttgccaatgg tggcttatta
    30901 cttaaaccgc aaatcgtcaa aacgatcaag actaaagatg gcattgaaat cttcaatgct
    30961 caaccggttt atatcagacg agttgccagt agtgaaacaa tgagcacttt acgcgagtta
    31021 ttaagactaa cagtaatgag tggaaccggt tcacgtgccg atatcaacgg attagccatt
    31081 gctggaaaaa ccggaactgc tcaaaaagtt gtcaacggag cttacagtca gaccgagtac
    31141 gtggcatcgt tcgtcggatt ctttccggca gataatccta agttactgtg tgtagtcgta
    31201 attgataacc cgaaaggtct caggcatact ggtggtggtg ttgcggcacc ggtcgttcga
    31261 gaagttttca aaagaatcgt gaaccagtct gacgatctgt tcttcgatga accagatccc
    31321 ttaccaaccc cagtggttaa ttatgtttac gaaactaaac cggcggtcgc taatgttcct
    31381 caaacacctg acgaggctta tcatcccaat ttaacgactg cgatttatac caatcaaatg
    31441 cctgatcttt gtggaaaaac tcttagacag gcggttggaa ttttgtatga actcggaatt
    31501 gacgatatcg aaattgacgg tgcgggagtg gtggtcagcc agaagccgac acctcattcg
    31561 ccaatcaatc cgcatgtcaa atgcatattg aaacttcaac ccactcggac gcctcttgaa
    31621 taattcactt tccgaaatat tacaaggtat tgaatttcgt cggctgggaa acttaccgac
    31681 ttcggtagcg gctcttagtg tcaattccaa ggaattggct aagggggcgc tctttttcgc
    31741 aattcgcggg gctaaggatg atggccataa atatgttaaa aacgcaatac aaaacggtgc
    31801 ggccgctgtg gtggtcgaag aagtagtcgc tgatgaatcc atcccccaga tcatcgttgc
    31861 cgacactaaa acagccctct caaaagccgc cgccaatttt tatgacaatc cgaccaataa
    31921 tatgacgatc gtcggtgtga ccggcaccaa cggcaaaaca actaccgttt acctattaaa
    31981 tgaaatattc aaagaagccg gtctaaagca cggtactgtc ggcacgctgg gttactcgat
    32041 tggagatgaa cgtttctcct cgaacttgac tacacccgat agtattcaat tacagcaaat
    32101 ttttaaaaaa atgctcaatc gcggagtcga aatagcggct atggaagtgt cttcgcatgc
    32161 tttagccttg catcgcgttg acgatgtcca ttttaaagcc gccgtgttta ccaatatttc
    32221 tcaagatcat ctcgattttc accatagtct cgatgaatat gccacaacta agagcaaact
    32281 cttcgcaatg attgaaaaag acgggtttgt aatcaataat ctcgatgata attattccga
    32341 tttattcaag aacgccgctc gatgccgcct gttcacctat tctttgctga agacagccga
    32401 tttcacctgg gcgcccggag taacctggtc aaacggcatt tccggagaaa tcctaactcc
    32461 caacggcaaa attgaaatca agagttgcct ttcgggacaa tttaacttga aaaatatttt
    32521 ggcggctacg gcggtagcgg ttaatctaaa tattgacgat aaaatcatcg cttccgcact
    32581 ttctggggtc gaatctgtgc ccgggcgctt acaagaaatt tcgcattctg gccaattgcg
    32641 cgtttttata gattatgctc atacgccgga cgccattact aatgtcttgc agactctacg
    32701 tggaatgctt ccggccgacg ggcgcttaat tgtagttttt ggttgtggtg gtaatcgcga
    32761 tcgcgataag cgtcctaaga tggcgcgagc cgtcgagtcc ttagccgacc aggcgatcgt
    32821 caccaccgac aatccgcgct tcgaagaacc gcatgatatt attgaggata ttgttaaggg
    32881 ctttacgagc gggtataatt tcaaagtaat cattgatcgg aaagaagcga tcctggatgc
    32941 tttaaaaaat gctgagcaaa acgatataat cgcaattctt ggcaaaggtc atgaaaccta
    33001 tcaagaaatt cgcggagtca ggtatccatt ttacgacggg gaaatcgtgc aacaattcta
    33061 cggcggatca aatgattaag gcagatttca ttcggtcact cggtctcgga cgcatagtca
    33121 atttcgaagg ggcaatcaat ggcgcggaaa ttgacagtcg caaacttcag cctggtgcgg
    33181 ctttcttcgc tttaaagggt gcgacatccg atggtcatgc ttatgttcag gaggctttgc
    33241 agaaaggtgc cgttttgtgc gtggtagcgg aagattgggc ggctaaaaat ccgactcccg
    33301 cacctctctg gatcgtaccc gatccggaaa aagccctcca gaaattagcc gaaaaatggc
    33361 gcctctcctt ctccattccg gtgctggcga ttactggaac taacgggaaa acgacaactc
    33421 gtgcgatgtg cgccgcaatt ataaaaactg cctataactt gcatacaact tccggcaatt
    33481 tcaataatca actcggtttg ccgatcactt tgttgaattt aagacctgaa caccaattca
    33541 gtctaattga aatggggaca aatcatttcg gcgagattgc ccgactatgt gaaattgccc
    33601 atccgaatgc cggtttaatt accaacgtcg ggtggggaca tattgaattt ttcgggaata
    33661 tcgaaggtgt agcgcaagcg aaatcggaat tgtttgccgc cttgccggaa aatggcaccg
    33721 cttttattaa tgcggatgat cctctaattg tcaaaatgcc ggtagccggg cgcaaaatta
    33781 agtacggttt tgaaaataaa gctgttgatt tctatggcaa aatctcaggc tataacgatg
    33841 aaggtcaagc ggttttaaac gtcaattcgc ggttcgacat tcatctgtca attcccggta
    33901 gaattgccgc tttaaatgcc ctagcggcgg ttgcagtcgg tttatttttt aatgtagatg
    33961 aaaaagcggt catagcggct cttgaagcat ttcaaccggt cagtcaacgc tttgtgcaga
    34021 ttaaagtcgg tccctatcag gtgattaatg atgcttacaa tgccaatcct gattcgacta
    34081 tcgccgcttt ggaaaccttt agacggatta aaacttctgg acgtcgcatt ttcataatgg
    34141 gcgatatgct cgaattaggt caatatgcta ttcaagggca taaacgagtc ggcaaggcgg
    34201 tcgctcaagc cgatattgac cgcttttatg gcgttggaac gctaactgcc tatgccattg
    34261 agactgccgc tcgggctggc ttaaaagccg cttattattt cagcaccaaa tccgatttat
    34321 tagccgcttt aaaatccgat ttgaaagaag gcgatactct actcgtgaaa ggttcacgcg
    34381 gcagtcgtat ggaagaaatt attgagggac taagccaata atatgtttta ccatctcctc
    34441 tatccattaa aaaaatactt cacaggattt aatctgtttc agtacatcac ctttcgagcg
    34501 gctatggcgg ctattacggc tttgctgatt agtttcatta ttggtccata catcattaaa
    34561 aagctaaaaa aataccaaat cggcgagttg attcgggaag atggccccca gacacatttg
    34621 gctaaaaaag gtacaccaac aatgggcgga attattatct tattttcggt aattgttcca
    34681 gtacttttgt gggctaatct taccaacacc tacataaatc taattttact tagtacaatc
    34741 tggatgggta ttttcggttt cctcgatgat tatttaaaag tcattaagaa aaagaaaaaa
    34801 gggttgatcg ctcgttataa acttttaggg caaatcactc tgggcttgtt aatcggttgt
    34861 tatctgtatt tctcacctga atttgctgac attaactcaa tcacttccgt accgttttta
    34921 aaaaataatt atcttgattt cggatggttt tatattccgt tcgtaatttt tttcatcact
    34981 ggtatttcta acgccgttaa tctaactgac ggcctggatg gtcttgccgc tggcttgatg
    35041 ggaatcgtcg ccttggctat ggcggtcatt gcttatataa ccgggcgtgt agattttagt
    35101 aattacttaa acacgattta tttggcggga agtggggaat tatcaatatt ctgtatggcc
    35161 gccgccggag catttcttgg tttcctgtgg tttaatgcca aaccggctga agtttttatg
    35221 ggcgacactg gctcactttc aatgggtagc gctctggcta ctctggcaat tttattgaaa
    35281 aaggaaatat tgttcatttt tctgggcgct ttgttcattg ccgaggcgct ctcggtcata
    35341 attcaagtgg tttactttaa atggactaaa aagcggaccg gcgttggtca acgggttttc
    35401 aaaatggcgc ccttacatca ccatttcgaa atgcagggca taccggagag taaaattgta
    35461 atccgctttt ggataatcgg cattttgttg gctttgttca gtctaagtac ttttaaaatt
    35521 ctatgataga tttaaaaacc aaaccagcta atctgaatga tttaaaagtc gccgttctgg
    35581 gcgctaaacg cagtggaatt gcggccgcaa tattagccgc ccgacggggt gctgaggttc
    35641 ttttatcaga taccgccaat ccggaaattg ccccagaaca gcattccgca ttaaagcaac
    35701 acaatgttca attggaaatt ggcgggcaca cctcgaaaat ttttgattcc gacctagttg
    35761 ttctaagtcc gggaattccc aatgatgctc ccatcgtcat tgaagtcacc aagcattcaa
    35821 taccgatcgt ggctgaagtg gagatggctt actggttctg ccctacggat aatatcatcg
    35881 ccgttactgg ttccaatggt aaaactacta ccactaccct tctatctgag atttttaaag
    35941 gcactcaata cgaaccgtat tgtggcggca acatcgggac ggcttttagc catttgatca
    36001 ttgaggctga acgttccgcc cacccccaga aaattttcat tctggagctg agcagttttc
    36061 agctggagcg cattgtccat ttccgtccga aagtttcgct gatcctgaac ataacgcccg
    36121 atcatctgga tcgctacgaa catcgtatgg agctttattt agccgccaaa ttgcgcataa
    36181 cgatgaatca gaccgaagat gactactata tttttaatga cgatgatgaa ttgctaaaga
    36241 ctaatttgcc gccgagatgc catttgattc ccgccggatt acattccact tcccagaaat
    36301 taattacttc agacgccgag aagatttatt tttccggaca agatcaattc attgaacggg
    36361 cgcgagttgc tttgctcggc gaacataatc tttacaattt gaccgccgct ttgactgccg
    36421 ggcgattgta tcaattacca attacgcatt tacaaaatgt attgtacaat ttcaaaagca
    36481 ttgaacatcg cctcgaattt gtgacaacca tagacggcgt ggactattac aatgattcca
    36541 aagccactaa cgttgattcg gtcaattatg ccctaaaaag ttttaaccgg ccggtaattg
    36601 tcattttggg cggaaaagat aaagactccg attttacgct tctcatcccg caaatccggg
    36661 agcatgtcaa agccgcagtt ttaattggaa aagccgctga taaaattcgc aaggcattga
    36721 gtggtgtcgt cgagatgtat gacgccgggt attctatgcc gaatgccatt gaaattgctc
    36781 gcaatctggc tgcgcctggt gacatagttt tgctctcgcc ggcttgtgcc agtttcgata
    36841 tgtttaatga ttatgaacat cgcggtcggg tttttaagga aatcgtgatg aatttgccga
    36901 aggacttgaa agtatgaacg ttgcttttaa aaaaggaaaa gttgaccaaa aatacttgat
    36961 cgcctttttt attctgttag gtttcggttt agtgatgcaa tatagtgcca gttcttcgct
    37021 tggggaagcc cgttttgacg atccgtcttt ttatgttcgt ggacagtttg ttcggattgt
    37081 gttgggtatg gttgttggtc ttatttgtac ttttatcaat tatcaaattc tcaaaaaagg
    37141 cgcgttctgg attgctctgg ctgggattat tgcactgatt gcgactttag tctatcacaa
    37201 attgcacccc ggtcaagcga ctgcgcgctg gtttccgatc ggcggtttta attttcagcc
    37261 ttctgaattc gccaaattgg cattaattat ttacctttca tccttcatcg agcgccacga
    37321 aagacatctg gatgatttca agcgtggttt tataccacca gtcatcatta tgttaataat
    37381 ggtgatattg attatcgtcg agccgaatta cagcacagct gctgttttta ctgttttcgg
    37441 tctaattgtt ttatttatcg gtggcacgaa actgctccac tttctaccct ttataggtgg
    37501 atttgcatta ttatcggtaa tcacaattct gcgttcaccg tataaactga tgcgcatcct
    37561 gacttttcta gaaccagaaa aggatttaca gggcgccggt tatcaaattc atcaatcaat
    37621 gatcacactg ggtaatggtg gttttttcgg tcgtggtctg ggcggtagta tcgagaaaaa
    37681 tttgtttttg cccgatatgc atactgattt tattttctcg gtagtcggcg aagagcttgg
    37741 ttttatcggg gcggtggttt tgctatcact ttacctttat ctatttatca ggggaataaa
    37801 aatagctctg cgcgcgccgg atgttttcgg caatttgcta ggtatcggtc tatcgagttg
    37861 tttgtttatt tacgtaattg ttaatgtggg tgttacctgt ggacttttac cggtaaccgg
    37921 cttgccttta cccttcataa gctacggcgg ttcttcgatg ctattcaatt tcgcctgtgt
    37981 cgggatcatt ctaaacattt cacgctatgc ggtcccgcaa gcaaaaacaa attcactggt
    38041 gatgttcaat gactaagaaa gatgaatctc gacgtttaaa agtattaatc acgggcggcg
    38101 gtaccggtgg gcatcttttt ccggcgatcg ccctggccga agaacttcag atgcgtcaat
    38161 cgcagattgg cgaactcgaa atcattttta ttggaacgcg gcggggcttg gaatctcaaa
    38221 tagttccgag tctgggtttt gaattccaca aaatctggat tcgcggctgg caacgcggct
    38281 ggactctaaa tgatattttg gtcaatctgt tatttcccgt cagattattc gtcagtttga
    38341 tacaatcatt ttttatcatt aaaaattttg atcccgattt agccattgga accggcggct
    38401 atacggctgg accacctcta agaattgccg caatgctgaa aatcccgatt tttctccacg
    38461 agcagaatgt cattccaggg gctacgacta aaatgctggc aaacaaagcc aagcggcttt
    38521 atacgtcttt tgaagagtca aaaacttacc tggaaaacgc catctgcctc ggcactcctc
    38581 tccggcgttc tctgcaaatt gttccgcggg agcaggcgat ttactttttt gaattggagc
    38641 ccgatcgcaa gaccgttttt atttttggag gcagtcaggg ctcccaagct attaattctt
    38701 attggattga gaatatcacc acggtggtgg aagccgttaa atgtcaattc atctggcaga
    38761 ccggacagcg cgattttaaa atgattgata acatttttag taacaatccc tgggttcacg
    38821 tcacgccttt cattcatgaa atgggaattg cctattgtgc cgctgatgtt gtcattagcc
    38881 gggcaggcgc tctatcgctg gcagaacttt gtctctatgg caaaccttcc attctcgtgc
    38941 ctttaccaac tgcggctggc aatcaccagg aaatcaacgc ccgcaatatg gaacagcgtg
    39001 gcgcttccat tttggtactc cagaaagaat tgactaacga ccgattgact caggaactta
    39061 tcgctttact gaaagatgag ccgcgtcttc aagctatgtc tgccaacgct aaagccttag
    39121 ccagaccgga ttcggcaaaa ttaatcattg acgatatttt aggttttatc gaagtaaatg
    39181 cactctgaag ttaaaaaata tcactttgtt ggaatcggcg gcataggtat gtgcggtctg
    39241 gctgaatatt tattgcacaa cggtcaaatt gtaaccggct ctgacctggc gccttccgaa
    39301 aatacagaac ggttggaaaa tctgggcgct caaatcaaat acggacatcg ccgcgaaaac
    39361 gtcggcgatt gtgactatct ggtatattct tcggccgtca agccggacaa tccggagaga
    39421 atggtcgcta tcgaacgtca aattccaact ctgcggcgag cggaattgct cggccaaatc
    39481 tttgatacta aacccattcg gattgcagtc agcggtactc acggaaaaac gacgacaacg
    39541 gcgatgatcg ggcaagtctt gactacggct ggtatggacc cactcatttt aagtggtggc
    39601 gttttgaaga gtagcggtag cccggttcaa atcggcaaag gtgatatagt aatcgccgaa
    39661 gccgatgaat ttgatcgcag tttcctgcaa ttatctccaa cgcatgccat catcacaaat
    39721 attgagagtg aacatttgga ttgctatgca aacctcaccc aaatcaaaaa tgccttttat
    39781 gaatttaccg ctaagatacc ggatgctggc agagtcatcg cctgctcgga tgaaaaagaa
    39841 atacccgact ttttaaaaca tcatccgcgg ccatatgtta cctatggtct ttatagcgga
    39901 gaatttcaag ctcgtaatat ccaattaaaa gggaattcaa gtgagtgcga agtttatcat
    39961 aattcagatt taattggctc gctccaatta cacgtccccg gtctacataa tattaaaaac
    40021 ggactagcgg cgatcgcgat ggcttcttta ctaaacatac ccttcccggt tatccaggaa
    40081 ggtttggcag actttcgggg cgtaaaaagg cgattcgaaa ttctacaaga aaacgacaat
    40141 cttatgctgg tggatgacta tgcccatcac cccagtgaaa ttgcggcgac tctcaacgcc
    40201 gctcgcaaag gctggcaaag acgaatcatt gcggttttcc agccccattt attttctcgt
    40261 actcgggatt tctatcccga atttgcgcgg gtacttcggg tggctgatgt tatcgttctt
    40321 cttgaaattt atccagcgcg agaagcacct ttagaaggta taagttcccg cttgatttat
    40381 gatgaaattt gcaaagaccg gactcaggac gtttacctta ccaacacact cgctgaagcc
    40441 ctagcattat tgcaagaaat aaaattaacc ggcgatatga ttatcacact gggtgccggt
    40501 gacatcaata aaattctact gaaattaatg gaataaaaaa ttgaaactgg gattgataat
    40561 attaaaaaat aaatcgcaaa ggtgtttagg tgttcagaat ttatcttctt ttttcagaaa
    40621 gtactgggac ctaaatagac tttgtgatca aaacctttgg acaaaaattt tccagggttc
    40681 ccgcagatat ggggggcaaa acatcaaaac gccggctctt cagcaccgaa aaatttctgc
    40741 aatcggttat cccacgaaca aagatttcga tctgaagaca cagcaacgcc aggtaatttt
    40801 gctattattc tcgggaaccc tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40921 nnncagattc agaaccggct tgagatggaa ccatatatcg aggcggcagt cgtcagtcgc
    40981 gaatttccga atcacttgaa aataatgatc aaagagcgca tcccagtttg ttacttgaat
    41041 acgggcacgc tctggctaat tgaccaacaa ggaatcatat taccgcttcg caaggaaaaa
    41101 ttgaaagcca atttgccggt tattaccggt ttcgagaaag attccgcccg ttgtcggccg
    41161 ggacagcttg cacctaatcc cgaaatgttc aaagccttga atttcatcca gactgccgcc
    41221 ctgaaggcca atactctgta taccgaaatt tcagaaatct ttgcctggaa aaagggcgaa
    41281 tttattattt atacggtcaa cggaggaacg ccgatttata tcggacgtgg cgatttaacc
    41341 aaacaattat atattctggc ggcttttcaa aacttaatca aatccaagcg cacttttgcc
    41401 gattatcaat atcttgattt gcgctgggat aaacaaatca tagcaaaaga acgaaaatca
    41461 taaatgaaaa tgttaaaact aaaaaaccaa atcctgaccg gtctcgacgt tggctcatct
    41521 aaagtctgcg ccgtaattgc cgaagttact cccgattcac ccgaacctat tattaaaggg
    41581 tacggtatca gtccttctaa aggaattaag aaaggcttgg tcgttgatat tgaaaatgcg
    41641 actcagtcaa ttgttaatgc cgttagcgaa gctgaaaaaa tggctggttt ttcgattaat
    41701 tcctgcctgg taggagtttc gggcgatcat atccgcagtt tgaatacacg tggaatgatg
    41761 gcaatctcgc gcgattcacg taccggctta ggcgaagccc gagaaatcga gatcgaagat
    41821 atcgagcgtg taatcgaaca caccaaggct gtaccgcttc cgttagatcg gcagattcta
    41881 catgttcttc ctcaggaata catcattgat gaacagccgg gagtaaaaaa tccgttgaat
    41941 ctcagcggtc gacggctgga tgccaaggta catctgacca cctataatac caccgttctc
    42001 tccaatttaa gtaaatgcat caagaatgcc gatctggaaa ttgacggctt tgtattgagt
    42061 tctctggcgg ccgcctatgc ctgtctggaa gagagtgaaa aaacgatggg cagtgttctt
    42121 ctggacattg gcagtggagt cattgacata atcgtttttt acgatgacgg tgttcaccat
    42181 accggagtcg tcaatctggg agctaatagc gttaccaatg atatcgctta tatgctgaga
    42241 gtccccctcg aaaaagccga aagtattaaa cgtgaatatg gttttgccaa agtctctttg
    42301 accgatagag aagcattttt cagactggat agtcttgggg ggcggtcacc tcgagaaatt
    42361 tctattttta atctggctga atggattgaa cctcgtatgg aagaaatttt gcgcgaatca
    42421 tatctcgaag ccaaaaaagc cgatattcct ttgactaata cattatcagt cgtcctgacg
    42481 ggcggcggcg cccggttaaa aggcaccgat gaattagctg aaatgatttt caatacccca
    42541 gcaagagtgg cagctccccg tggtttccag gaatatgtca cggaattaag tgatccgatt
    42601 tattcggtgg caatcggctt aattaaatat gcaattgccg gacaaattag cggttcggct
    42661 ttaaataaaa caatcagcgg tccctttcac agaataaggg gctggattaa acacgtaacc
    42721 gaaaatgtaa tgtaattagg gaggcttaaa atgttatttg aatttgaaga attagccaat
    42781 caacaagcca gactgaaagt ggtcggcatc ggtggcggag gcggaaacgc cattaccaga
    42841 atgattgaga atggactggt cggggttgat ttcatcgctc taaatactga tgttcaggcg
    42901 ctcgaatgct gtaaagcccc aactaaattt cagatcggac gagctctgac gcgtggtctg
    42961 ggtgctggtg ccattgatga cattggaaga aaagcggctg aagccgatcg gcagacgata
    43021 gcttcaatga ttgaagatac cgatatggtt ttcattactg ctggaatggg aggtggcact
    43081 gggactggcg ccgcaccaat cgttgcccaa attgcacggg aacaaggttc attgaccgtc
    43141 ggaatagtca ctaaaccttt taatttcgaa ggggcgaagc ggatggcacg cgccgaagag
    43201 ggtattaatc aactcaggaa atgcgttgat actctaatcg taattccgaa ccaaaaatta
    43261 ttggcaattg ttgaccacga tacttccata ttggatgttt ttaaaactgc tgatagcgta
    43321 ttatatcaag cgattcgtgg catttcagac ctgattaaca aaaatggcgt tattaattta
    43381 gattttgctg atgtcagaac cattatgaaa gatatgggta aagccattat gggcactggc
    43441 ttggcaaccg gtgaggaacg cgctgttctt gccgctcagc aagccatttc cagtccatta
    43501 cttgatgaag ttaatatcaa agatgccaaa ggtctgctaa ttaacattac cggaccggct
    43561 aacctcgaca tccatgaagt tgacgaagcc tgttcaatta taaccgatga ggttggtgcc
    43621 aacgctgaaa ccatctttgg tatggtagtg gacgaaaatt acaccgacga aattatgatc
    43681 accgtaatcg cgactggttt tggtgaagcg ccgacgccga ttaatctgtc ccaaaataaa
    43741 aaggatttct cctccccaat taaagacgaa ccacgaaata ctgacgatat ttggaataga
    43801 attaatgaac cgggacagcc tgttagcctc ttcagtgatg accaggtcat taaaatcctg
    43861 aatcctgatg atatgggtat ggctgataat ccttcccata aaatacacca gaccaatatt
    43921 ccaacttata tacgtcaaaa aataaaatag ttcgatttat ttttctcccc ctattaaaat
    43981 ccaaaagccc ctgtttattc aggggctttt agtttgaatc cttagagtat taatatcaga
    44041 cggctttttg aattgtaccg ctcttgagac aggtcagaca aacttttaca gttttaattg
    44101 aaccactatt atccactatt ctaattttac gcagattggg ttcccatatc cgatgagttc
    44161 tattattagc atgactaact ttattgccgt gaatcggctt ttttccgcaa atatcacaaa
    44221 cttttgacat tttcattact ccttaaaaag cgtgcaaatt tgccgaaaat aattggaaat
    44281 atcaaataat ttctttattt cccgtaggac agcctttcca ggctgtcttt atccgactgt
    44341 cctacaaaaa gaaaaggctg tcagggataa ccttccgggc tgtgtcttta tccgacaggc
    44401 aagaatgcct gtcctacgaa aaggaaaagg ctgtcaaacg acagcctttc cctttacaga
    44461 gcctcaggaa ttgcctattt cattaatacc atcttatggt tcttacggga gtcgccagcc
    44521 tgcattacac agatataata gccggaactg acctgcattc catgattatt ccttgcatcc
    44581 cagatgactg acttataacc agcttgctgt ctctcattga ccagagtgcg aatttcccgt
    44641 cccataatat cataaatgac caatttgacg tgtacttctt caggtaggtc gtattttatc
    44701 gtggtaatcg ggttaaacgg attcgggtag ttctggtgca gggcaagaac ctttggaacc
    44761 aaatcacctt tgattccttc cggagtaaga ttaatggtaa aatcaaccgt atcggaactg
    44821 ctgaaagcct cccaggtatc ataagccaga acatacgcct gataattacc attatccttg
    44881 taagtcggcg catcaatcag aatggatgta tccggtgttt caaccgagat gaccaccgag
    44941 tctttatccg tattcaagat aaataattta taagtcagtg cctcattatc cgggtcaacg
    45001 gctctcgacc attttacctc aatcagtgaa tcctgaacaa tatcgctatt agtaacttcg
    45061 tagccttcag taggctttag catagtaaag ggtaaaggcg gggtgttcat cttaaggata
    45121 aaattgaccg tatcggaact ggtgaaattc tcccgagtat cataagccag aacatatgcc
    45181 tgataattac cattatcttt gtaattcggt gcatcaatca gaacagatgt atccgctgat
    45241 tcagtcgagg tagccaactc attccgatcc acattaaaaa tgaataattt ataagtcact
    45301 gcattaccat ctggatcaac ggctttcgtc cattttacct caatcagtga atcctgaaca
    45361 atatcgctgt tggtaacttc gtagccttca gtaggcttta gcatagtaaa gggtaaaggc
    45421 ggaatattcg agcctgctgt ccacattgaa aaggcatcaa tacctgtgag agtaatttta
    45481 ttattggcta catcccgtat tcctcctctc tcaacccagg ttttaccagt atcagctgaa
    45541 ctatatagta atagtcgctc ttccgacaag ccattcaatt cactctcatc ataattgaaa
    45601 actaaggtag cgtttaatcc ggtattagtc gttggagtaa tcatatacca acgacttaca
    45661 cttaatttac tattgactga atcagcagta aatccgcgct ttatcactgt cgaacctaaa
    45721 tcagcgcttg aagtaatcac tgctcctaag ttaccaacat taaccgcgga aggtgcggtc
    45781 aatacttgtg tttcagaaat cgttccgttt ccagtagctc cttcccaggt aatcgtcttg
    45841 ccattcaaag ccaaatcacc atttgttagt ctcagggtat cacatgcagc tgacccggac
    45901 aaggtcacac cagcagtgtt attaatgaca agatctttaa ccgtcgctgg caaatcggga
    45961 ccggttactt gagctacggt accattataa acgtaattgg cagcagtgct aaacgtccgg
    46021 gtagaagtac gaatcggtcc ggcaaccgga tcaagtccag ctggtacccc aatattcaac
    46081 gtcgcaccat cacctaaaat aaaactgcct gttccatcta caaaactatt ctcatttgca
    46141 aaagtagccg ttccgttcac tgtaattgaa ccatcaatag taactcgact aacaccttcg
    46201 gttgctccag tcgttaaaaa tctatcacca ataacaattg aaccggttga gccaactatt
    46261 aatgtcgctg tatctccagc agccgatagt aatgtaaacg aactattagg ctgagataaa
    46321 tccatagtgc catttacaat gaatttgacg ctctgtgacg aagcactgga tatactactg
    46381 ccaacgccta tattagcatt atcaacaaat gtaatcgttt taccggcatt gaaaattatt
    46441 acattgttat cggtttgcgg ataaatggct accccaccac taccactact accggtgtac
    46501 ataaatttcg tatcaatatc gaagattgtg gtatctccac tcacattgct atttactctg
    46561 aagcgagaag gtgcaaaagt tccagtacca gttatgacta tttttccacc actccttgca
    46621 ttctcaaggc aaatgttatt tcccggacta tttggatcac caaaagtgcc gcttactact
    46681 gctgaatcac cattgatccg tacatacctt tgatcacttg ttggatttgg tttgtcagct
    46741 ttgaaagtcg ctccggcttc aatgtacaga tttttacagt tcttcccgga agcatccaga
    46801 ataacgtcat aaccattcct gatggtgaca ttattatctt tacctggtgc cgcactggct
    46861 actacccagt tggtaccatc aaatttttcc caagtgcttg atacactcca tttcccacca
    46921 ttaacggccg aacgatagtc tccaacactt tgtgcccagg ctaccgaggc cgcaattaaa
    46981 caaaatgtaa ctaaaaataa caatgaacgt ttcataacct ctccttaaat tttaattgtc
    47041 atcatcattc tcaaaatata tcaaaattgg ttctttctca ttagcttggt tagtatatca
    47101 atttttagat ttttacaatt tagcatcaca tcaaagataa agtaaaatcg cgtaaattct
    47161 ctattgatat ctgaattaat tttcatattt atctttccac tcacattcta actttatgaa
    47221 ataagacatt aaaagtcaag agttgtatta caaattttga aatgtttaac atcttttgtc
    47281 gc
//
LOCUS       DAOOMU010000006        41693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_687_length_41693_cov_18.539075, whole genome shotgun sequence.
ACCESSION   DAOOMU010000006 DAOOMU010000000
VERSION     DAOOMU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 41693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41693
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_687_length_41693_cov_18.539075"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1185
                     /locus_tag="PLI55_01155"
     CDS             115..1185
                     /locus_tag="PLI55_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99479.1"
                     /translation="MARKIVHIIGTGTVGAPLIGVLLGHLEQLGIEEISFEPNLKAIE
                     NKAFLNGLVTRGAKLCVREEIADEFISVGCKIDYTSKEAIERAGVIIDCSPGDEPLKN
                     KAEIYSRLDGEPKFFIAQSRAAGFGLDYAYGINDEALVSSEVQFVRVVSCNAHNIAAM
                     IKTLAFDEKGENYLDWGRFVCIRRASDISENKPFIPAPQISLPQDDNYGTFQALDTAS
                     LFRTIGVNLDIFSSALKVNSQYLHIVNFDLKLKRPVTLPLVINKLESNSLIALTNKTQ
                     SSLVFAFARDMGFLGRILNQSVVSVPSLTVKEDREIIGFCFSPQDGNSILSSIAATVW
                     YFYPHSYRDKLQNLDDLIFAEV"
     gene            1347..1979
                     /locus_tag="PLI55_01160"
     CDS             1347..1979
                     /locus_tag="PLI55_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPA99480.1"
                     /translation="MLIEQIITQQFSENVWLVGDPQTRKGLIIDPGDHADEIIGKISA
                     MNIQPLAILNTHAHPDHLAAAAEVQNHYNIPFYLHAKEKAVLKSAIPFGLVLGIRITR
                     VPSQVIYLQDEETLKIEPFEIRVIATPGHTPGSVCYLIADRLFSGDTLFRNSVGRVDF
                     PGSSWQELKRSLEKLKNLPPTTEVYPGHGMNTTIGQELSHNPYLKNGEWR"
     gene            1976..3370
                     /gene="tilS"
                     /locus_tag="PLI55_01165"
     CDS             1976..3370
                     /gene="tilS"
                     /locus_tag="PLI55_01165"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPA99481.1"
                     /translation="MKPTTLQRKFVEYVEQNHLLNDVRTVVAAVSGGVDSMCLLDLLM
                     SEKKRWDIEIVVAHFNHQIRGPEADSDENLVRQFCDNHSLPFFSAAENVPKIARAKRI
                     SLEMAGRELRYAFFQKIAADFPNSIIATAHTLDDQAETILLRMLKGTGLQGLSGIAMR
                     RENIVRPLLFAAKAELYDYARARELPFAEDHTNLCNDYERNFIRNQILPTVQHTLNPN
                     IRATLANLAQNLGEAHQFIVEFAEKALESLIIKANSEQIVLDKSGLKTYFIAVEKEII
                     WQSLQRLDGTIKPLNFKQMNQLIKLVNEGETGRRMNLGGEITVLIDRERLILKRNRSS
                     NWSSSPVIPGEKIKNEFFTFSSELMDVSNFKKGKRRSGVEFIDFDLVGNNLGLRHWQR
                     GDRMIPLGMNQSKKISDILVDLKVPFTDKNSIPIFYSGDKIIWLCGLTLSDEFKVTSK
                     TKTVLKLIYKGKKI"
     gene            3367..3924
                     /gene="hpt"
                     /locus_tag="PLI55_01170"
     CDS             3367..3924
                     /gene="hpt"
                     /locus_tag="PLI55_01170"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPA99482.1"
                     /translation="MKKTITLDASYGPYAGQVIEELLTVAQINRRVTELAAEISAMYQ
                     GTVPIFIGVLNGSFIFFADLFRKLTIDGEVDFIKISSYADQTRSSGTILLRKDISCDI
                     TGREVIIVEDIIDSGLSIKFLKRRMEESNPCSVRFAALLMKEDARVDFPIDFVGFKIP
                     NKFVVGYGLDVAQRLRNLASVYAFK"
     gene            4095..6023
                     /gene="ftsH"
                     /locus_tag="PLI55_01175"
     CDS             4095..6023
                     /gene="ftsH"
                     /locus_tag="PLI55_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPA99483.1"
                     /translation="MWIIIILSSIVLLQILGRGAQEEVVITYSQYKELLDQKLIKSAV
                     IIENEFHGTLTQKVPLVVGGRTIEAEKILVNLRFVDSEMLKSWDEAGIEYDFKVKNTK
                     WTNFFINIFPLLFIILMTLFFLRRMQGQNNKGIFGFGKSKARLWTSDRPKVSFNDVAG
                     CAEAKEELQEIIEFLKSPSKFSRLGGKIPKGALLLGPPGTGKTLLAKAVAGEAGVPFF
                     SISGADFVEMFVGVGASRVRDLFEQGKHSAPCIIFIDEIDAVGRQRGAGLGGGHDERE
                     QTLNQLLVEMDGFDENTNVIILAATNRPDVLDTALLRPGRFDRQIVVDMPDLVGREGI
                     LKVHTKKIPLADDVDLSVLARGTPGLVGADLANLVNEAALLAARKNKKAVEMIDFEEA
                     KDKVMMGVERRSLHISDRERRLTAYHESGHVLVAVNIPEADPIHKVTIVPRGRALGIT
                     HQLPMDERHNYSRSYINSRLSILLGGRAAEKIIFNELTTGAGNDIETATELARKMVCE
                     WGMSDKLGPLTFGKKNEEIFLGREIATHRDFSEETARLIDQEIREIVENAEKQACEII
                     KKNIDLLHKLALALLEKEVLDGAEIAKILGNSEVNSANIFVNKSDDNMTKPATKAKTG
                     SRRRKSEKEKGANTDQAA"
     gene            6055..7200
                     /gene="folP"
                     /locus_tag="PLI55_01180"
     CDS             6055..7200
                     /gene="folP"
                     /locus_tag="PLI55_01180"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPA99484.1"
                     /translation="MESLDVDPAGIAIMVRKSEFLILKTDPIIAPAANILKQQILSVG
                     GDCAVAREVPTGQAPPTPVILMATRRQYERLIINLKQQCFGLDKLAVEIEEFLVRISA
                     PRKFHIGQQEYQTDQKTLIMGILNVTPDSFYDGGRHAEMSAAIETALEMEADGADLID
                     IGGESTRPGSDPIPAEVEIARLKPVLEQLQMRLKIPISIDTYKSAVAEWALNNGALII
                     NDISGLNFDSQMAGTIAKHNAGVILMHIKGTPKNMQADPHYTNLIDEIMKYLSDSVQK
                     ARTAGIDGSKIFIDPGIGFGKTAEDNYRIMRYLAEFQSLGLPILVGPSRKAFIGQVLN
                     LPADERLEGTLAAVTAAVLNGADLVRVHDVKAAVRAVKIADAIKGKS"
     gene            7201..7977
                     /gene="cdaA"
                     /locus_tag="PLI55_01185"
     CDS             7201..7977
                     /gene="cdaA"
                     /locus_tag="PLI55_01185"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPA99485.1"
                     /translation="MTLFTIKFLSVTIWDVIDIIVVYIIILKLYQFFKGSRASQMLIG
                     LAIILLTSFVARALNLQSLSWIMGNLQTIWAIAFVIIFQPELRRILIYLGQSRFVRRF
                     LSESKSKTIEAVIEASRELVRRKWGGLFVLVREVGLKPIKERATPINAEVSASLLVSI
                     FNPGSPLHDGAVLIQNDIIEAAGCILPLSDSPDLDPQMGTRHRAALGISEESDAVVVV
                     VSEETQKIAVAYQGMLYRNVDEDSLRALLSRLFFRGSNNS"
     gene            7999..>11837
                     /locus_tag="PLI55_01190"
     CDS             7999..>11837
                     /locus_tag="PLI55_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPA99486.1"
                     /translation="MSIKPVPFYLSHNIKHPLRILFCGFILLISQNLLWSAVSDIEIV
                     EKWLNATTWQWQAELPMPKFPVDEKNQTCAIEISGLENLARDGYPSIPVYQKIINALP
                     DEIAFTVQTGLEKVVPLPAVMSISQDIPIAGEGTNQEKIILPKSKNFSAYPMRLVEIE
                     FLGYLKGIPLSSVKIFPCQLIEQGKRLKYFGSVTVQVQVKPGTRLQKSVATDEQALLK
                     ALDIAPGSIRKIAKQSPTLAKPAVTFPAGKVLAKLIVDEDGIYRVSCRALSDSGIALK
                     KVDPRTFRLFNQHHEVPIYVYGESDGIFHNNDYIEFFGQRNRNSVADYEYDPFTDKNV
                     YWLTWGENYGLRYAEESVQVNSPIDETVVPIDYLYTAHIEVNAAFERLGQVDVDQPTH
                     VRDHWFFDSGIFGGTAREYIFHLSYPNPNQINNFNAQLRMQGLTYGDAGDHQADYFIN
                     NNKVASGAWNGQKAYTLENSASQVLHNNFLKHGENVLRVQVSGADPTDKYSKVDVDYL
                     KVKYFRLYKADEDELDFTRPESSIDGLYTFTLSNFSTSDISIYKVGKSKLRDFTTEYI
                     KSTNSYRVMLQDYVRDDTTLYWAACGEALKFPLSVKLDTIIGLSSIPSGAEVVVITRQ
                     EWVNNLSKLTDFYASIGLSSQVVGIEDIYNEFNAGIISPFAIKKFLQYVCYNWNPVPE
                     YILLVGDADIKEERSIPSYFFQSYSYGACASDFWYALIDDDDVPDYAIGRWPCSTIEE
                     LEALIDKRLNYTKHNLIGPWNNELLFIAGQENVFKNQSENMIQRQISKEFNINRIYVN
                     PASVGSRFYGGSDTLLHLFNNGITLGNFMGHGGGAVWADRSLFTLHHIPELENLDRLP
                     FLTSMTCFTGDFTNVTGLGEMLLLAENGGAIGLWGASSVGWIKNDYLLAKPFYDVIFE
                     PGMTVGKAIQTAKIRYQAEAFDYLRLSLIYSYNLIGDPTVSLPFPEQSTLLNLSQTNP
                     APGDTVILRGDLPFEAGKMYIQLFDSSKYAVYEKPAEVPFSSSNLHHEIILPTNINPG
                     NTYVNYYIESKTAKQDAHGATLFDIKGLTFYGFDYSPSTPGEKTPIILSIYTELSDVQ
                     SFFCEIDTVSAYEYLDGDGIEHIISFQNDSVLLSIRMGEVESVANKWQTTQVFQVGTP
                     GKLVAVRFVAIDSSGNRTVSPAYSIRIKSLPDLRPIGIAQGGERFPELIAEVSYNGND
                     TLSAVVKAERRVDEKFELFGSKEVVFLPNRATSVKLPGILGKDTVIFRITVDPENTVA
                     ESNEMNNSLSQSMVINT"
     assembly_gap    11838..11937
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11938..12752
                     /locus_tag="PLI55_01195"
     CDS             <11938..12752
                     /locus_tag="PLI55_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPA99487.1"
                     /translation="TVDLSNCPAASQIDDLNIGKWNPSLRIWYNEEGQKEGMKIKTTS
                     TIPGTFSLITNTDTKPPNLELNLEGQQFFQNSYVSDRPSISIIGEDDNGVRFDAEGIK
                     VIIDNNEVALENLHAPDTVSSGHYFSVQFRPTFTHGNHSMEVTLKDAAGNSTTESVDF
                     IVAEELKLIDYGNYPNPFRNRTTFIYELTQRVNSLKIKIYTTSGRLVRVLDSENIFES
                     GLDLNEGGYHEVVWDGLDADGNFVANGIYFYKISAKSGNKTVTKTGKLAKAR"
     gene            12749..13759
                     /locus_tag="PLI55_01200"
     CDS             12749..13759
                     /locus_tag="PLI55_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99488.1"
                     /translation="MKRILIIGIILISLGSGLVAGEYADAFLELGVSARASAMANAIG
                     ALDFSETAFITNPAGISYIRQARLGLMYTSQFGLADHNYIGLAVPAGDHYSLALSWIR
                     YGVDDIPIRPDIFTTITDKAERRDSIISIYNGKLPTFNDVENAVFVSLGRFNSTIVDL
                     GWRYSKFKLEFPIGINLKFIHKKYYELEGFGLGVDIGGRLRVDGEELLDVSNLGMLSV
                     GMALKDVTGTTIYWNTKSQDRIRLTPALSFALEQPIKPLKLIINLVSEKQYRYHDKTN
                     YGVEIIYANSLALRAGLKNSGISLGLGLNFKVMKKLINLDYSFYSHDLGASHRVGLGI
                     GI"
     gene            13765..14520
                     /locus_tag="PLI55_01205"
     CDS             13765..14520
                     /locus_tag="PLI55_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99489.1"
                     /translation="MTTSNQINLMVALILLLNTCGKNLEDPDPPAQPEWVAKSLPYDT
                     TETGIDAVPNGDFIQLEWYTGDEDDLSSYRIYRASGDIENKFDLIEEIQSDLMAGSVN
                     IYLDENVSINTPYFYFLRAVDQAGNLSLRSDTVEYKLVVKVNLRGLFDTTDVNPTFKW
                     SDAGSAASEYVIRVEQFNPNRVMCLSATSRQSYTDEVQSLKYGTGIIFYSAQDTLSRG
                     VDYRWRVDAVATFNREGDEVAGSESNWGYFKIQ"
     gene            14536..15054
                     /locus_tag="PLI55_01210"
     CDS             14536..15054
                     /locus_tag="PLI55_01210"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPA99490.1"
                     /translation="MKRVSLFMILTLLSVQLYSQESQTITTRSGRTADRLSASKYILS
                     TGSDAMLMTVKIWGEVTRPGLYDVPIGTDLIDLISAAGGPTTRANLSKVKLIHGSPDE
                     EENYVTIVNIKKFLETGDVDMIPEIKPSDTIVVPVKITQHFLTSLSWTQQLLSLFSIY
                     SMILYYTSIANR"
     gene            15072..15539
                     /locus_tag="PLI55_01215"
     CDS             15072..15539
                     /locus_tag="PLI55_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HPA99491.1"
                     /translation="MIDSIDVQILDILQKEGRIQRNKIAEQVNLTIPAVSERMRKLEK
                     KGIIEGYHAKINSAAIGKDVTAFVFVMTSPSKNYDEFVRRSREESDIIECHSITGEGS
                     HLLKVQTDNTSALEKLLSKIQSWPGVLQTRTYIVLSSYKDFSSLKPASSLVKK"
     gene            15609..16725
                     /gene="prfB"
                     /locus_tag="PLI55_01220"
     CDS             join(15609..15671,15673..16725)
                     /gene="prfB"
                     /locus_tag="PLI55_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPA99492.1"
                     /translation="MLSDIQVELDNIAPRIDELRRFFDVESKKNELNTERQKTTASDF
                     WSDPAQAQTTLQKIRGLESEIDNFENLVKYQKEITDFVDLAAEEQDDSVLAELEKMVN
                     ELISKVEQLELETMLDEPDDEKNAILTIHPGAGGTESQDWAHMLMRMYLRWMERSGME
                     ADVISILDGEEAGIKEVTIEVKGRYAYGYLKAEAGIHRLVRISPFDANHRRHTSFASV
                     FVYPAIEDDISVEINQDDLRIDTFRASGAGGQHVNKTSSAVRITHIPTGIVVQCQNER
                     SQFKNKDSAMKILRARLYQLKKEEKNKEKMEIEKNKKDISWGNQIRSYVFQPYTLVKD
                     HRTKYETGNIQAVMDGEIDEFLRQYLLFNVKNERAKK"
     gene            16722..18221
                     /gene="lysS"
                     /locus_tag="PLI55_01225"
     CDS             16722..18221
                     /gene="lysS"
                     /locus_tag="PLI55_01225"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPA99493.1"
                     /translation="MNKEEQATGKSLNELLAIRREKLQAIREMNFRAYEYEFQKTHSA
                     AEITQNFDELTEKVVVRVAGRLMAIRKMGRASFGHIQDESGRIQIYVQENSVGQQGYK
                     LFNLLDIGDIIGITGRVFRTRTGEITILVEELQLLAKNLRPIPIVKEKDGQIFDAFSD
                     KEQRYRQRYLDLMVNPEVREVFKTRSKIIQWTRDFLNEHGFLEVDTPILQPIYGGANA
                     RPFKTYYNALEREFYLRIAVELYLKRLIIGGFEKVYEIGKNFRNEGLDRSHNPEFTLL
                     EFYQTYVDYNYMMDFVEQYFHYLCSKLGESKFPFGEYEIDFSQPFSRRKMFDLIHEYV
                     GEDITQYSEAELKSLCLKKNLELKPGDNYGKYIELLFSNFVEPHLIQPTYVIDYPKAI
                     SPLAKNKRDGSPNLVERFELYIAGHEFVNAFSELNDPIDQRERLEQQNRLRESGDEEA
                     QTMDEDFVTALEYGMPPTGGVGIGIDRVVMLFTNQVSIKDVILFPQMRT"
     gene            18234..19454
                     /locus_tag="PLI55_01230"
     CDS             18234..19454
                     /locus_tag="PLI55_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA99494.1"
                     /translation="MSVPFFIAKRHLFSRHKIGYISFISIIATISLAVGVAALILTIS
                     ILNGFEQEIKTKLLGFDSHIRLRLLYQDSMDSTLAIRSELEKMPEIECFVPYIHGAAM
                     IRKGQETEGIIVEGIDEVHIRQTLAVDRFIKKGELRFNLPDGRDGIVIGAKLARQLGV
                     DIGDKVYLFVFQPEESYIKRPRIMTFTVTGLYDSGIADYDDVFIYTSLQAAQKLYDLG
                     EKFSGYQIRLKDQSKVEAVTEYINKTLGYPYNALSWNDLHSNLFEWLRIQRIPIMLIF
                     GLIAVVAVFNIVSSIMMIVIEKTRDIGILKSVGMNNRQVKRIFLYEGLFIGVVGTLAG
                     FGLSILLGYLQNRFKLISIPADVYFMSSLPVLLELRNFLIIGLIAIASALLATLYPSY
                     KAVKLTPSEATRYE"
     gene            19447..20670
                     /locus_tag="PLI55_01235"
     CDS             19447..20670
                     /locus_tag="PLI55_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPA99495.1"
                     /translation="MNKNFLNWIALRYFRSKKKTGLISFTSYISIIGIALGAFAMLVA
                     LSVLNGFEAEITNRVIDIESHLRISGRNLQSAQIERVKEALSGVPVREIYPFVMKKSI
                     LSSEQMEAVVRIKAIESATLEQQLSGPRTMMRGGANFLSATSNLPGIVVGYQLADKLG
                     VYPGDTLNVINPTSLGSGLNIPIIGQFAVTGVFQLDLFDYDENIAFIELKVGQEIFEM
                     GDNYSGIDIRLENYRYVEKAKQQLVAQLGNEYDVASWEDLHRTLFGAMKLEKYGSFLA
                     LSFIILVAILNLTSALVMLIMEKIQEIGMLQALGMNQTQVRQIFLRLGFLIGSIGLTV
                     GIGLALILCLAQQHFQFIPLPSVYYIPYLPVQVQWSDVLMIIGAGLIFIYCGTLYPSW
                     RVGKLMPLEAIQYEK"
     gene            20660..21361
                     /locus_tag="PLI55_01240"
     CDS             20660..21361
                     /locus_tag="PLI55_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPA99496.1"
                     /translation="MKNENDKTLLTVKNLSKSYWNDGLEIAVLRNINLTVKEGEVVAI
                     VGPSGIGKSTLLNLLGTLDRPDSGVIEYEGLNPFDLSDTEIARFRNTHIGFVFQFHHL
                     LPEFTALENVMIPAMIYNRDVRKAEEMAREMLARVGLAERATHRPAALSGGERQRVAV
                     ARALINNPLIILADEPTGNLDAMNSELLIELMLELNRIYRRTFIIATHNQTIAEKSHR
                     ILKFTKDSLLIEASI"
     gene            21509..23977
                     /locus_tag="PLI55_01245"
     CDS             21509..23977
                     /locus_tag="PLI55_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPA99497.1"
                     /translation="MKENFSKIIQIIVKAARYEAGRMNHSYVGSEHLLWAIINMERSV
                     TLEILMALDVDIKALQTMLTDSLKAIGGTATIGQIPFTKQVERILRNTYNEAKELGAE
                     VVGDEHITLSILKEKEGISYEILNSFGVDYYNFRAEISGNMDEPLPVTKSEKKSSRTP
                     ALDHFSRDITEKARTNQLDPVIGRDQEIERVAQILSRRKKNNPVLIGEPGVGKTAIAE
                     GLALRIVAKKVPHLLANKRIIDLDLAAIVAGTKYRGQFEERMKTIMQELEQARDVILF
                     IDELHTIVGAGGASGSLDASNMFKPALARGELQCIGATTLDEYRKFIEKDGALERRFQ
                     KVIINPPSKEESLEILKGLRERYEAHHHVKYTDEALWTAVELSDRYITDKFLPDKAID
                     VMDEAGSRVHLRDVIMPEEILEAEKKLEEVRNQKDNAVREQQFEKAADLRDSERRVKK
                     QLADLQQAWEKSGEKQTLLVTAEDIADVVAMMTGIPVNRIAESENQKLLTMEDELRKS
                     IIGQDDAIRVVSSAIRRARAGLKDPRRPIGSFLFLGPTGVGKTEFARVLARFLFPGTD
                     ALIKIDMSEYMEKFAVSRLIGAPPGYVGYEEGGELTERVRRQPYSLVLFDEIEKAHRD
                     VFNILLQIFDEGVLTDSFGRRVDFKNTIIIMTSNAGSRSLQSSAFGFSKADPLSEYEA
                     MKKRLLEEIQHVFNPEFLNRIDDPIVFKPLTREDVLAIIDLQLEDTRRNLGASGTTLE
                     ITQEAKELILTQGFKPEFGARPLRRAIQNMIEDPISELILAGTLRRGSKIVVSVTDNK
                     IVFTQPEIEFKISSTKITSEPVSN"
     gene            24118..26040
                     /gene="dnaK"
                     /locus_tag="PLI55_01250"
     CDS             24118..26040
                     /gene="dnaK"
                     /locus_tag="PLI55_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPA99498.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGKPRVLENSEGRPVTPSVVVFKK
                     DGEIVVGDTAKRQIVTNHDRAVYSIKRFMGRLYKEVQREREEVPFKVVEGPNGDARIR
                     IGDRDYSPPEISAMILQKMKQTAEEKLGEKITDAVITVPAYFNDSQRQATKDAGKIAG
                     LEVKRIINEPTAAALAYGLDKKKEEKIAVYDLGGGTFDISILEIGDGVFEVKSTNGDT
                     HLGGDDFDQRIIEWLISEFKKKEGIDLKKDPMAMQRLKEAAEKAKIELSSNLETEINL
                     PYITAGSAGPIHLMEKLSRAKFESLVDDLIERTRKPCQQALEDAGLKPEDINEVILVG
                     GSTRIPKVQDLVKDLFKKEPNKSVNPDEVVAIGAAIQGGVLAGEVHDILLLDVTPLSL
                     GIETLGGVTTKLIERNTTIPTRKSEIFSTAADNQTSVEINVLQGERAMAADNKSIGRF
                     HLDGIPPAPRGVPQIEVTFDIDANGILNVTAKDKATGKEQSVRIQTSSGLTEEEKEKM
                     VRDAQLHEEEDRRRKEEVDTRNQVDALIYQTEKNMKEYGDKLQPEEKGRLNNALEDLK
                     KAQNSGDVKTMKSAIEAMNQAWSEVSSKMYERVRTETPPPHTGQTYEQPHEETTGQKD
                     ENIQDADFEVVDDDKK"
     gene            26161..26233
                     /locus_tag="PLI55_01255"
     tRNA            26161..26233
                     /locus_tag="PLI55_01255"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26194..26196,aa:Cys,seq:gca)
     gene            26268..26352
                     /locus_tag="PLI55_01260"
     tRNA            26268..26352
                     /locus_tag="PLI55_01260"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26302..26304,aa:Leu,seq:taa)
     assembly_gap    26547..26646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            26718..29210
                     /locus_tag="PLI55_01265"
     CDS             26718..29210
                     /locus_tag="PLI55_01265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HPA99499.1"
                     /translation="MESSKKQSVINEKPTPIKGLTTEEALSRLKKYGPNTFPRSKPPS
                     AWSILISQFKSPLIYIILVAAVISLIMGELTDFIIIIAVVIVDVILGFFQEFQAQRTY
                     TALNKLLKPTTTVIRDGERQEIDVTKVVPGDIVILNAGERIPGDGEIIEGARLSVDEA
                     ILTGESEPIKKSVEAGSNQVFMGTTLLTGRGVMRVTQTGVRTELGKIAKSLQEHKEEE
                     TPLQVRLKSFSRTLTIIVVSFTTIIFLVGLLLKRDFLDMLRTSVILAISAIPEGLLIA
                     VTVILVLGMRKILKRQGLVKKLLAVETLGSVNVICTDKTGTLTEGRMRVTRTDFVDNE
                     RAIQIMVLCNNLEGPVEMALWEYAKSRLPNDPQTLVEESQRLDEEIFTSETKFMITGH
                     HLPDGDYHFLKGAPEIVLNMCQINDGERQRILAQIDQWAGEGLRLLGLAYRDYGILED
                     HTGYTWVGLIGMEDPIREGVHEAVLLAKHAGIQVKMITGDYRRTAERIASNIGLLQNG
                     EETLEGEELAQLNDEELAKRIYPTAVFARIRPHDKLRIVNALQKHGDITAMIGDGVND
                     APALKQANIGVVVGTASDVAKETADLILLDNNFRTIVAAIEEGRVIFENIRKVVAYVL
                     SNSFAEVLTIFISMILGWPAPLLVAQILWIHLICDGPADIVLGFEPKENGIMDEKPKS
                     LKEPILPPLGLFLIGVISIGSALAALLIFGHYFKIHGDADEGRSLVFASLAINSLIYI
                     FAYRSMRKSLFKMNSILKNKPLIFAVIFGMVLVFLPFVIPSLSQALGVVPLDLKQWLL
                     IFTIALMLLIAVEIGKAVSNWWHNKKNTLTKG"
     gene            29355..30800
                     /locus_tag="PLI55_01270"
     CDS             29355..30800
                     /locus_tag="PLI55_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA99500.1"
                     /translation="MAKIDPNSDYYSELSQFTIESDVDAILWIDAESTIYRVNQAACQ
                     LLQYSPEELVGKNLDDLIDIQNDWNKTDPHRFLDQIDIIKQNGVTVIERNFRTKDGRI
                     LTLEISAHLFVFKNNEYICVFARDITERRKAQEELQKTLKELEFLKNKLYEENIYLQN
                     EIKLTHNFEEIVGKSLSLRKVLEKVEQVAATDATVLIQGETGTGKELIARTIHHISSR
                     SSSPLVKVNCATLPAALIESELFGHEKGAFTGALYRKMGRFELANGGTIFLDEIGDLP
                     LEIQSKLLRVIQEGEFERIGSNKTLKTDVRIIAATNRNLEAAVQKGEFRKDLYYRLNV
                     FPIHVPPLRERPEDIPLLIHHFVQKYSKKIGRKIDIIPAKIMRTLTSYSWPGNIRELE
                     NIIERAVIITNGRTLQIGNWFTTDAPVMNKEKIQSLREAERDYILKVLSLTNWRVSGP
                     HGAAQLLDINPQTLVSKMKKLGIHRTKPINE"
     gene            30945..31298
                     /locus_tag="PLI55_01275"
     CDS             30945..31298
                     /locus_tag="PLI55_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPA99501.1"
                     /translation="MKVKKENKSIKKEDPKIIDMSTFDELIHSNKPVVVEFYDEKIST
                     SYIVDSILSRLANYFQNQIEFLRLHINQHRAIADKYHIFSSPATLIFLNGKLIEVILG
                     TFSQNQIIEILNRLL"
     gene            31320..32732
                     /locus_tag="PLI55_01280"
     CDS             31320..32732
                     /locus_tag="PLI55_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA99502.1"
                     /translation="MTTFKKYVNKIALDKLVTYCIKDPQKHIPKLIDFVDKSAWLPIH
                     KSYVQGAKSIVNNPDSNWYQLAMRAIRDIDPDIIHKIVRNLFINSGFEGQGKLVEERE
                     RLGFGIPWAVLMDPTSACNLKCKGCWASEYDKSLNLDTDLMDRIIREGKELGIYFYIY
                     SGGEPLIRKNDLINLAEKHSDCVFLSFTNGTLVDEHFADELRRVGNFSLALSIEGDEK
                     ATDFRRGKGSYQKLISAMKILKSRGIPFGFSTCYHSQNWNIVGEPEYLDQMIEAGCYF
                     GWYFTYIPLGKHADLSLLARPEHREHMYYRVREARQTKPIFLIDFWNDGEYVNGCIAG
                     GRRYLHINANGDVEPCAFIHYSDTNIKNVSLLEALQSPLFLQYQKNQPFNQNHLRPCP
                     LLDNPDALKSIVLETGAYSTQHMDREDVVELTAKCQDIAQAWKLTADRLWEETLKNRV
                     QKKSGVKQYERELVDAEIHN"
     gene            33470..34576
                     /locus_tag="PLI55_01285"
     CDS             33470..34576
                     /locus_tag="PLI55_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPA99503.1"
                     /translation="MTANSQAENLSHLTLKEAFKDDFKIGAALDLNQISGRDEQGLAI
                     VEKHFNSITPENILKWEEVHPEPDRYNFDAADQFVAFGEKHNMQIIGHCLVWFFQTPD
                     WVFEDGAGKPLSRAALLARMKEHIFTVMGRYKGHIHGWDVVNEALMIDGTLRKCKWLD
                     IIGEDYILKAFEYAHEADPEAELYYNEYDTEHKSKREALVRLIQDLQAKGARVDGIGT
                     QGHWFLDYPSREEIEKAILEISTTNVKQMITELDVGVLPFYPVDSQMVDISSFDPEMQ
                     KKYNPYPDGLPDSVQKELARRYAELFTIFLKHSDKIDRVTFWAVHDKQSWRSYLPIRG
                     RVEYPMLFDRQCRPKPALEAILKVAQNRAKLVEN"
     gene            34673..35890
                     /locus_tag="PLI55_01290"
     CDS             34673..35890
                     /locus_tag="PLI55_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPA99504.1"
                     /translation="MEKKKNLEIVAAGHTCLDLIPAFTIEGEVEKMTDVLVPGKMINM
                     GECVIAGGGPVTNAGVSIRRLGVKTELIGKVGNDDFGRLVLDWYEKNEGHFKGIKMVD
                     GESTSYTIAICIPGIDRFYLHHCGANDTFGYDDMDFNLVGRAKLMLFGYPPWMRKIYE
                     NTGAELTKILKKTKELGTTTALDLSLPDVDSYAGQVDWKAILTDWVPLSDIMVPSAEE
                     LFYFLYKDKFLEKRDKLRHKETVLDQMTVKEISTMGRDLIRMGAAIAMIKCGSRGLYI
                     YTAGQERLKKMGAAGCADLENWANRELWFPVYQEEKFVGALGSGDSAIAGFLSAFVWG
                     HSIESCLRYANAAGSMNVTVPDGLSWNKGFDDLTRRIKAGWKTKEMKINEPGWKKERE
                     FWVGPDNRGKWES"
     gene            35908..37725
                     /locus_tag="PLI55_01295"
     CDS             35908..37725
                     /locus_tag="PLI55_01295"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HPA99505.1"
                     /translation="MSKERDVFIQKKIAGMTLMEKIGQLLTFTRRGTLLTPSGIEQIT
                     RLHCGGLCLEPYAVETCKNLYWGNSQIDKNFQPPKDYFTIANNYFDGKTFGISITPED
                     YTKDLNRLQKIAMSRPSGIPLHMTIDFEGDFKNDYMSGGIRQFPGPMGMAAIGDEKLT
                     YEIGKTIARMLTAIGVTQMYSPVCDVNTNPRNPEIGVRSFSDDPEVCARHAVALIRGL
                     QDYGIAATAKHFPGRGDSGSDAHDVLEVLNLDKKRMHEVELVPFKAAINAGVKAIMTG
                     HSVYPAFDDKYPTTLSEKILTGLLREELGFEGVIVSDAIGMAAILKQWPLPQACAMAI
                     KAGCDTILLKADDESRSQCFFGIKMAVERGELSEERIDQSVARLLKMKYDQGLFESAG
                     EKDPEITREITTAKEVIDFSWDVAKKALIVMRDEQHLLPLDPQQRILVIEQRIPYEFL
                     GKDPYCHTHMFCEAMVNHSQNLILADTAFSAYEDEIKECLELAKQADLVVMTNYYARI
                     VKSGNNRLLVKKLKEAGHKVIVVTNNPYIEGTTVDADAVVCNFSGTPDSIRVSTYLLY
                     GKVIPCPTTKLPIKPDVGEKTPVKDLKAPKKHPLNLSYC"
     gene            37939..40416
                     /locus_tag="PLI55_01300"
     CDS             37939..40416
                     /locus_tag="PLI55_01300"
                     /inference="COORDINATES: protein motif:HMM:NF016563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HPA99506.1"
                     /translation="MTVHFQGMSKTLQFPQTIALEGAPTCSIGISPEGLYNRLNDAGR
                     LILLNQNAIQFGSAPFIKIAADPNAEDERDEYFMLTTRDPNIVEGPDDVPALKNYYHP
                     PVPEAKVTISLATPLAEIIYDLAKISVTRLITSPLFSGNDQTLIPISVEEFVVENTSD
                     KTQEVTLVIPRPSLVNLQEKELKPTDQDTVYICSASVKGQVHEAFNSAGIKGVVMGNQ
                     ECQEKMAIAVPEIEGVDIDTHPHFCLNHLKQDLLLNDDGSFYEKRNVIPHQDYGAAIS
                     LHFTLGPKASLKIPFAIALDFPQQRFIDGVEFERKYVKLFKNQASRTIEMIKVALENY
                     PEWLNRTLTIQNRIYELIRKNPAYRDDEEGALRLARLIFNEFSCPISNACVWVKDNEG
                     KERARFLECFDYAYINPSDVDWYSMVLLFLFPKIEEEICQGFINSILAEDPTPRFYHL
                     HASFVEARQHFEKYPEEYEDISLTQIWDATKVKGSVAHDLGALPKGHALRNVSDYTWY
                     NNNYWIDLFPKLATRVLRNVKFTGNTAFVKENWETLKFGFDHLQELDFDGDGIPEGYP
                     GEVKNTFDNLTLFGVDAYDATIFMAGCYAMIKMAEIVKDTAAKKQYEEIFDKVSKEFE
                     KLWRDTKNSQGKRLQYYVTCFDPKTGETNTDVWMNQLDAIWALIAMGEESFIPEERIK
                     QILKTVYDNNRAYMGWAMCRTEDGGKVESEQGQDVYTTSNYVFAQLLDYYGMTEESKE
                     VYKAMDKVIFGHGNTLISPDNLRAELEQEAGESAPGPHYIVAAYPRPGAILTQIVLQY
                     IKETQKQTKNMLVDSDKLKSFAISLMK"
     gene            40480..>41693
                     /locus_tag="PLI55_01305"
     CDS             40480..>41693
                     /locus_tag="PLI55_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA99507.1"
                     /translation="MKEKTGVLSLKEKIGYSLGDTASNLFFQTFIFFLLYFYTDVFGI
                     PASSVATIFLIGRIWDAVNDPLMGAIADRTNTKMGKFRPYLLYTALPFGILGFIMFIT
                     PDLSVQGKVIYAFITYNLMMMVYTVINVPYSALMGVISPHSEERTVVSTFRFVAAFVG
                     QFIVQFAVLRLVSVFGKGNEQIGWQWTMAVMAGLSVVLWLVTFATTKERVKPVKGQKN
                     PLKQDLADLLRNVPWLLIGSATIFQLLFNVIRGGSIMYYFKYFVQDQQLFLFGKPHSY
                     SYQTLTSTFMLTGTLITIVGSLLTSFFSKRIGKSRSYWGFLGISAICSALFFFLKPQD
                     VVWMFLLQIIASFCMGPVSVLQWAIYTDTADYSEWRTGRRATALIMAVSLFALKLGIA
                     LGGSILAWILDI"
BASE COUNT        12213 a         8027 c         9568 g        11685 t
ORIGIN      
        1 tcctacggct aatataattt tacttttaac aattttttat cgcaaaattt tgtgttaatt
       61 tataaggcta ttttggattt aaaatgacga taatttaaca ggagtaaaaa ataaatggcg
      121 cgcaaaatcg tgcatataat tggtacggga acagttggcg ccccgttaat cggcgtgtta
      181 ctcggtcacc ttgaacaatt gggaatcgaa gaaatctcct ttgaaccaaa tttaaaagcc
      241 atagaaaata aagcgttcct gaatggattg gtgactcgcg gcgcaaaatt atgcgttcgc
      301 gaggaaattg ccgatgaatt tataagcgtc ggatgcaaaa ttgattacac atcgaaagag
      361 gcaattgaac gggcgggagt gattatagat tgttcgcctg gtgatgagcc attgaaaaat
      421 aaagccgaaa tttattcgcg cctcgatggt gagccaaaat ttttcatcgc tcagtccaga
      481 gcggccggtt ttggtttgga ttatgcctac ggcatcaacg atgaggcttt ggtatcgagc
      541 gaagttcagt ttgtccgggt tgtttcctgc aatgcgcaca atattgccgc tatgattaaa
      601 actttggcat tcgatgaaaa aggcgaaaat tacttagatt gggggcgctt tgtgtgtatt
      661 cgccgggcga gtgatattag tgagaataaa ccttttattc ctgcaccgca aattagcctg
      721 ccccaggatg acaactatgg aacttttcaa gctttggata cggcttctct gtttcgcacc
      781 ataggagtta atctcgatat tttttcatcg gcactgaaag tcaattctca atatctgcat
      841 attgtcaatt tcgatctaaa gctgaaacga ccggttactt tgcctttagt gatcaacaag
      901 cttgaaagta attcgctcat cgctctgaca aacaagaccc agtccagtct ggtattcgcc
      961 ttcgcgcgtg atatgggatt cctcggtcgg attctcaatc aatcggtggt ttccgtgccg
     1021 agtcttaccg ttaaagagga tcgggaaata ataggattct gcttttcgcc ccaagatggc
     1081 aattctattc tcagttcgat tgcggctact gtctggtatt tttatccgca ttcttaccgg
     1141 gacaaattgc aaaatctgga tgatctaatt tttgccgaag tctgaatttg ttaaccgcag
     1201 aggatgctaa gaacgccgag taattattga acaaacgtaa taatagtttt gaattaaata
     1261 aggtcaaatt atacacctta agtattttgc tttacgtatg attatatgac tcgggtttcg
     1321 ttttttaaat ttcgaataaa ctcaatatgc tgatagaaca aatcattact caacaatttt
     1381 cagaaaatgt ctggctggta ggtgacccgc aaacgcggaa aggattgatt attgatcctg
     1441 gtgaccacgc cgatgaaatt atcggtaaaa ttagcgcgat gaacattcaa ccgctggcaa
     1501 ttttgaacac gcatgcgcat cctgatcatt tagccgccgc ggctgaagtg caaaatcatt
     1561 acaatatacc gttttatctt catgctaaag aaaaagccgt tttgaaatcc gccattcctt
     1621 ttggattggt gctgggaata agaattactc gagtccccag tcaggtgatt tatctacaag
     1681 atgaagaaac acttaaaatc gaaccttttg agattagagt gattgcaacg cccggtcata
     1741 ctcccggcag tgtttgttat ctgatcgccg atcggctatt ttccggtgat acgttgttcc
     1801 ggaattcggt cggtcgcgtt gatttcccgg gcagttcctg gcaggaattg aagcgcagtc
     1861 tggagaaatt aaagaatttg ccgccgacaa ccgaagtcta tcccggtcac ggtatgaaca
     1921 ctactattgg gcaggaatta tctcataatc cgtatctaaa aaatggcgaa tggcgatgaa
     1981 acctaccact cttcagcgga agttcgtaga atatgtcgag cagaaccatc ttttaaatga
     2041 tgtgcgaacc gtagtcgccg ccgtttcggg tggagttgat tctatgtgcc tgctggattt
     2101 attaatgtca gagaaaaagc gctgggacat cgagattgtc gttgctcatt ttaatcatca
     2161 gattcgcggt cctgaggcgg attctgacga aaaccttgtc cggcaatttt gcgataatca
     2221 ctcattgcca ttcttttccg cagctgaaaa tgtgccaaaa atcgctcgcg ctaaacggat
     2281 ctccctcgaa atggctggac gggaattgcg ctatgctttt tttcagaaaa ttgcggctga
     2341 ttttccgaac tcaatcatcg ccacggcgca tactctcgat gatcaggctg agacgattct
     2401 gctaagaatg ctgaaaggca ccggtttgca aggattatct ggaattgcaa tgcggcgcga
     2461 gaatattgtt cggccgttgt tatttgcggc caaggcggaa ttgtatgact atgcccgggc
     2521 gagagaattg ccatttgccg aagaccacac taatttatgt aacgactacg aacgtaattt
     2581 tattcgtaat cagattctgc cgacagtaca acatactcta aatcctaata ttagagcgac
     2641 tttagctaat ttggcgcaaa atttaggcga agcgcatcaa tttattgtcg aatttgccga
     2701 aaaggcgctg gagagtttaa ttattaaggc gaactccgaa caaatagtgc ttgataaatc
     2761 tggtcttaaa acctatttta tcgcagttga aaaagagata atctggcaat ctttacaaag
     2821 gttagatggc acaattaagc cattaaattt taagcagatg aaccaattga ttaaattagt
     2881 gaatgaaggt gaaaccggac ggcgtatgaa tcttggcggt gaaattacag ttctaatcga
     2941 tcgagagcgg ctgattttaa agcgcaatcg gtcgtcgaat tggtcatctt cacccgttat
     3001 ccccggtgaa aaaattaaga atgagttttt caccttttcc agtgaactga tggatgtaag
     3061 taatttcaag aaggggaaac ggcgctcagg agtagagttt attgactttg atctggtcgg
     3121 aaataatctt ggtttgcgac attggcagag aggcgatcgc atgataccac tcgggatgaa
     3181 ccaatccaag aaaatcagcg atattttggt ggatttgaaa gttcccttca ctgataaaaa
     3241 cagcattcca atcttttata gtggagataa aatcatctgg ctctgcggct taacactgtc
     3301 ggatgaattt aaagttacct cgaaaactaa aaccgttctg aaattgatct acaagggaaa
     3361 aaagatatga aaaagactat aactctggat gccagttatg gcccgtatgc cggccaggta
     3421 atcgaagaac ttctgaccgt cgcccaaatc aaccggcgag ttacagaatt agctgctgaa
     3481 atctctgcga tgtaccaagg aaccgtaccg atttttatcg gggtattaaa cggcagtttc
     3541 atcttttttg ccgatctatt ccggaaatta acaattgacg gtgaagtaga ttttatcaaa
     3601 atttcttcct atgccgatca gactcgatcc tccggaacga ttttactgcg gaaggatatt
     3661 agctgtgata taaccggtcg agaggtaata attgtcgagg atataattga ttccggatta
     3721 tcaatcaaat ttctaaaacg tcgtatggaa gaaagtaatc cgtgctcggt tcgcttcgcg
     3781 gcgttgttga tgaaagaaga cgcgcgggtg gatttcccga ttgattttgt aggatttaag
     3841 ataccaaata aatttgttgt cggatatggt ctggacgttg cccagagact acgcaactta
     3901 gcgtcagttt atgcttttaa gtaagaaaaa aaaggaattt taactatatg tctgaaaata
     3961 actaccctaa aaaccaaagt tcaaaaaatt taaatgaacc gaggaagccc cgtcagccga
     4021 ggcgaagacc tccagaaccg cctaaaccaa ataaagacga tttccgctgg aaaaaggctt
     4081 cacgcacttc cctgatgtgg attatcataa ttctcagttc gatcgttcta ttacaaattc
     4141 tcggacgagg cgcccaagaa gaagtggtca ttacctattc gcaatacaag gaattgctgg
     4201 atcaaaaatt gatcaaatcg gcagtgatta tcgagaatga atttcatggg acgttgactc
     4261 aaaaagttcc cttagtagtt ggcggcagga cgattgaagc cgaaaaaatt ttagtcaatt
     4321 tacgttttgt tgatagtgag atgctaaaaa gctgggatga agccgggatc gaatacgatt
     4381 ttaaggtaaa gaataccaag tggacaaatt ttttcatcaa tattttccct ttacttttca
     4441 ttattctgat gactctattt tttctgcgtc gaatgcaagg gcaaaataac aagggcattt
     4501 tcggttttgg gaaaagcaaa gctcgcctct ggaccagcga ccggcctaaa gtcagtttca
     4561 atgatgtggc gggatgtgcc gaagccaagg aggaattgca ggaaataatt gaattcctga
     4621 aatcgccgtc gaaattctcg cgtctgggcg gcaaaattcc caaaggtgcg ttattgctcg
     4681 gtcctcccgg aacgggaaaa actcttctgg ctaaagcggt agccggtgaa gccggagttc
     4741 cgtttttcag tattagtgga gctgattttg tggaaatgtt tgtgggcgtc ggcgcctccc
     4801 gagtgcgcga ccttttcgaa cagggtaaac atagtgcgcc atgtataatt tttattgatg
     4861 aaattgatgc cgttggacga caacgtggtg ccggattagg tggcggtcac gatgaacgcg
     4921 aacagacatt gaaccaattg ctcgtcgaga tggatggttt cgatgagaat accaatgtca
     4981 ttattttagc ggcgactaac cggcccgatg ttttggatac cgccttgttg cggccggggc
     5041 gcttcgatcg gcagattgtc gtggatatgc ccgacttagt tggacgcgaa gggattttga
     5101 aagtgcatac taagaaaatt cccctggcgg atgatgttga tttgtcggtt ttggcgaggg
     5161 gaacgcccgg tctggtgggc gctgatcttg ccaatctcgt taatgaagcc gcgcttttag
     5221 ccgcccgtaa aaataaaaaa gccgttgaga tgattgactt cgaagaagct aaagacaaag
     5281 taatgatggg cgtcgaaagg agaagtctac atatttcgga tcgggagcgc cggctgaccg
     5341 cgtatcatga atccgggcat gttttagttg ccgtcaatat tccggaagcc gacccgatcc
     5401 acaaagtaac cattgtgccg cgtggtcggg cgctgggaat cactcaccaa ttgccgatgg
     5461 acgagcggca taattattca cgctcatata tcaatagccg actcagtatt ctgctcggcg
     5521 gacgcgccgc agagaaaatt atcttcaatg aattgacaac tggagccgga aatgatattg
     5581 aaaccgcgac ggaactggca cgcaaaatgg tttgtgaatg gggaatgagc gacaaactcg
     5641 gtccgcttac tttcggtaag aaaaatgaag aaatcttcct cggtcgggaa attgctactc
     5701 atcgcgattt cagcgaagaa accgctcgtc tgattgatca agaaattcgt gaaatagtcg
     5761 aaaatgctga gaagcaagct tgtgaaatca tcaaaaagaa tattgattta ctacacaaat
     5821 tagccttagc tttactcgaa aaagaagttt tagatggcgc tgaaattgct aaaatcctgg
     5881 gtaattccga agttaattct gctaatatat tcgtaaataa aagtgatgat aatatgacta
     5941 agccagccac taaagcaaag accggttcac gacgaagaaa aagcgaaaaa gaaaaaggag
     6001 ccaatactga tcaggcggct taaaaacgac gcgctcaatc acctcgtcac tgaaatggaa
     6061 tcactggatg tcgatccggc gggaattgct attatggtta gaaaaagcga attccttatc
     6121 ctaaaaaccg acccgatcat cgccccagcc gccaatattc taaaacaaca aatattgtct
     6181 gtcggcggcg attgtgccgt agcgcgtgaa gtgcccaccg gacaagcacc accaacgccg
     6241 gtaattttaa tggcaactcg tcgtcaatat gaacgcttga taatcaatct caaacagcaa
     6301 tgcttcggac ttgataaact cgcggttgaa attgaagaat ttttggttcg aatttccgcg
     6361 ccaaggaaat ttcatatcgg tcagcaagaa taccagaccg accagaaaac cttaattatg
     6421 ggtattttaa atgtaacgcc agattcgttt tatgacggcg gtcggcatgc ggaaatgtcc
     6481 gccgctatag agaccgctct ggagatggaa gccgacggcg ccgatctgat tgatattgga
     6541 ggagaatcaa cccgtccggg ttccgatccg attccagccg aggttgagat tgcccgacta
     6601 aagccggttt tggagcagtt gcagatgcgc cttaaaattc cgatttcgat tgatacttat
     6661 aaatcggccg tggctgagtg ggctttgaat aatggcgcgc tgataatcaa tgacattagc
     6721 gggcttaatt ttgattccca gatggcgggt acaatcgcca agcacaacgc cggggttatc
     6781 ctgatgcaca tcaaaggaac tcccaaaaat atgcaagctg atccgcatta tactaatttg
     6841 attgatgaaa taatgaaata cttatcggat tcggttcaaa aggctcgcac tgccggtatt
     6901 gacggctcga aaatatttat agatccgggg attggattcg ggaaaacagc cgaggataat
     6961 taccggataa tgcgttattt ggcagaattc caatcactcg gacttcccat tctggtcggg
     7021 ccatcgcgga aagcctttat cggacaggta ctcaatttac cggctgatga gcgattggag
     7081 ggaactctgg cggctgtgac agccgcagtt ctaaatggcg ctgatttagt gcgtgttcac
     7141 gatgtaaaag ccgccgttcg cgcggtaaaa attgccgatg ctataaaggg aaaatcataa
     7201 atgactctgt ttaccatcaa atttttaagc gttaccatct gggacgtaat agatataatc
     7261 gtcgtttata taataatcct taaactctat caatttttca aaggctcacg tgcctcgcaa
     7321 atgctaatcg gtctggcgat tattttactg acctcttttg ttgccagagc gctgaatttg
     7381 cagagtctat cttggataat gggtaatctg caaaccatct gggcgattgc ctttgtgatt
     7441 atttttcagc cggaactgcg ccggatttta atttatttgg ggcagagtcg ctttgtcaga
     7501 cgatttttaa gtgaatcgaa gtccaaaacg attgaggcgg tgatcgaagc atcccgggaa
     7561 ttggtgcggc gtaaatgggg tggtctattt gtgctggttc gggaagtggg tttgaagccg
     7621 ataaaagagc gggcaactcc catcaacgct gaagtctcag ccagcctttt agtttcaatc
     7681 ttcaatcccg gatcaccatt acacgatggg gcggttttaa ttcagaatga cattattgag
     7741 gcggccggtt gtattctccc gctttcggat agccctgacc ttgatccgca aatgggtact
     7801 cgccaccgcg cggcgttggg tattagtgaa gaatcggatg cggtggtagt agttgtatcg
     7861 gaggaaaccc aaaaaattgc cgttgcctat cagggtatgt tatatcgtaa tgttgacgag
     7921 gattccttaa gagcattgtt aagccggctg ttcttcagag gcagtaataa tagctagatg
     7981 ggcaacaggt aagtaattat gtcgataaaa cctgtcccat tttatctatc tcataatatt
     8041 aaacacccac ttcggatttt attctgcgga tttattttac taatcagcca aaatttattg
     8101 tggtcggcgg tttctgatat tgaaattgtg gaaaaatggc tgaacgctac gacctggcaa
     8161 tggcaagccg aattaccaat gccaaaattt ccagtagatg agaaaaatca aacctgtgcc
     8221 attgaaattt ccggtctgga aaatttagcg cgtgatggct atccgtcaat ccctgtctat
     8281 caaaaaatta ttaatgcttt accggatgag attgctttta cagttcaaac tggtttggaa
     8341 aaagttgtgc cgttaccggc agtgatgagt atatcgcagg atattcctat tgccggagaa
     8401 ggaacgaatc aggaaaagat tattctcccc aagtcaaaaa attttagcgc ttatccgatg
     8461 cgtttagtag aaattgaatt tctcggttat ttgaagggca tcccactgag cagtgtcaaa
     8521 atttttccct gtcaattgat tgagcaaggc aagcgcttaa aatattttgg ttcagtgacg
     8581 gttcaggtgc aagtcaaacc agggacccgg ctccaaaaaa gtgttgcaac tgacgaacaa
     8641 gcacttctaa aagccctcga tattgcaccc ggttcaatca gaaaaattgc taaacagtcg
     8701 ccgactttag cgaaaccggc ggtgactttt ccggctggta aagttttagc taaattgatt
     8761 gttgatgagg atgggattta tcgcgtttct tgtcgggcgc taagcgacag cggcattgct
     8821 ttgaaaaaag ttgacccgcg tactttccgc ctatttaatc agcaccatga agtaccaatt
     8881 tacgtttatg gtgaatctga cggaatcttc cacaataatg actatatcga attttttggc
     8941 cagcgcaatc gcaacagtgt ggctgattat gaatacgatc catttaccga taaaaatgtt
     9001 tattggttga cctggggtga aaattatggt ctgcgttacg ccgaagaatc ggttcaggtg
     9061 aattctccga ttgatgagac ggttgtacca attgactatt tatatactgc ccatatcgaa
     9121 gtcaatgcgg ctttcgagcg ccttgggcaa gtggatgttg atcagccgac tcatgtccgc
     9181 gaccactggt ttttcgattc ggggattttt ggtggtacag caagggaata cattttccat
     9241 ctgtcttatc ccaaccccaa tcaaattaat aacttcaatg ctcaattaag aatgcaggga
     9301 cttacctatg gcgatgcagg cgatcatcaa gccgattatt ttataaataa taacaaagtt
     9361 gctagcggtg cctggaatgg tcaaaaggca tatactctcg aaaacagtgc tagccaggtt
     9421 ttgcataata attttctgaa acatggcgag aatgttctgc gagttcaagt ttccggtgct
     9481 gatccgaccg acaaatatag caaagtagac gtggattatt taaaggtaaa atacttccgg
     9541 ctatacaaag ccgacgagga cgaactcgat tttacccgac cggaaagctc gattgacgga
     9601 ctttatactt ttacactttc caatttcagc acttcggata tttcaattta taaagtagga
     9661 aagagcaaac tccgcgattt tacgacggaa tacatcaaaa gcactaactc ctaccgggta
     9721 atgcttcagg attatgtgcg agatgatacg accctctatt gggcggcttg cggggaggcg
     9781 ctgaaatttc cgctttcggt aaaattggat acgataatcg gtctaagttc gatcccttcc
     9841 ggtgctgaag tggtcgttat tacccgtcag gaatgggtca ataatctttc caaattgact
     9901 gatttttatg ccagcatcgg cttgtcatct caggttgttg gaattgaaga catatataac
     9961 gagtttaatg ctggaattat cagtcccttt gcgatcaaaa aattcctgca gtatgtttgt
    10021 tataactgga atcccgttcc ggaatatatt ttgctggtcg gcgatgccga tataaaggaa
    10081 gagcgctcca tcccttctta tttttttcaa tcttatagct acggagcctg tgccagcgat
    10141 ttctggtatg ctctaataga tgacgatgat gtgcctgatt acgctattgg tcgctggcca
    10201 tgttcaacca ttgaagaatt ggaagcacta attgacaagc gcctaaatta taccaaacac
    10261 aatctcattg gtccctggaa taatgaactg ctatttattg ctggacagga aaacgttttc
    10321 aaaaatcaat cggaaaatat gatccagcgt cagattagta aggaattcaa tatcaatcgc
    10381 atttatgtta atccggcttc cgtcggttcg cgtttctacg gtggctcgga tacgctccta
    10441 catttattca ataacggtat tactttaggt aattttatgg gacacggcgg cggtgccgta
    10501 tgggctgacc gctcgctttt cactttacat catattccgg aactcgagaa tctggatcgt
    10561 ctgccgtttc tgaccagtat gacctgtttt accggtgatt tcaccaatgt gactggatta
    10621 ggtgaaatgc tccttttggc tgaaaatggc ggagcaatcg gtttgtgggg agcatccagt
    10681 gttggctgga ttaaaaatga ttatctattg gcgaaacctt tctacgatgt catctttgaa
    10741 cccggaatga cggtaggcaa agcgatccag accgcgaaaa ttcgctatca ggctgaggct
    10801 tttgattatt taagactttc attgatatat tcttataatt taatcggtga tccgacggtt
    10861 agtctgcctt ttccggaaca atctaccctg cttaacctta gccagaccaa tccggcaccg
    10921 ggcgatacgg ttattctgcg tggcgacttg ccttttgaag cggggaaaat gtatattcaa
    10981 cttttcgatt ccagcaaata tgcggtatat gagaaaccgg ctgaggtgcc gtttagtagc
    11041 agtaatttac accacgaaat aattttgccc actaatatta atcccggtaa tacctatgtt
    11101 aattattata tcgaaagcaa gactgccaaa caagatgctc acggagcgac gctgtttgat
    11161 attaaaggct tgacctttta tggttttgat tattctccat ctacaccagg ggaaaaaaca
    11221 cctataattt tgtctattta taccgagctc tcggatgtcc agtcgttttt ttgcgagatt
    11281 gatacggtca gcgcctatga atatttggac ggagatggca ttgaacatat catcagtttt
    11341 cagaacgatt cagttttatt atcaattcgg atgggtgagg ttgaatcggt cgctaataaa
    11401 tggcaaacca cccaggtttt ccaagtaggc acgccgggga aattagtagc cgtccgcttt
    11461 gttgcaattg actccagtgg taatcggaca gtcagcccag cttattcaat taggataaaa
    11521 agtttaccgg atttacgtcc gatcgggatt gcccaaggcg gcgagcgttt tccggaattg
    11581 attgctgagg tctcatataa tggtaatgac actcttagtg ctgtcgtcaa ggcggagcgc
    11641 agagttgatg agaaatttga gttgtttggt tcaaaggaag tggtattttt accgaatcgg
    11701 gcgacctcgg taaaattacc aggtatattg ggcaaagata cagtcatttt tagaattaca
    11761 gttgacccgg aaaatacagt cgccgaatcc aatgaaatga ataatagtct gtcacaatct
    11821 atggtgatta atactttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntga
    11941 ccgttgattt gagtaattgc ccggcggcga gtcaaattga cgatttgaac attggcaaat
    12001 ggaatccttc cttgcgtatt tggtataatg aagaaggaca aaaggaaggt atgaaaatca
    12061 aaacaacctc gactattccg ggtacattta gtctaattac caatactgac acgaaaccgc
    12121 ctaatctgga attaaatctg gagggtcagc aatttttcca gaattcgtac gtttccgacc
    12181 gaccgagtat ttcgattatc ggcgaagatg ataatggcgt acgctttgac gccgaaggta
    12241 taaaagtgat aattgataat aatgaagttg cccttgagaa tttgcacgca ccggatacgg
    12301 tttcgagcgg gcattatttt tcggtgcaat tccgtccgac gtttactcat ggtaatcatt
    12361 cgatggaggt aaccttgaag gacgcggctg gtaatagcac aaccgaatcg gtggatttca
    12421 tcgttgctga ggaattaaaa ttgattgatt atggcaatta ccccaatccc tttaggaatc
    12481 gcacgacttt catttatgaa ctgacgcaga gagtaaattc actgaagata aaaatctata
    12541 ccacttccgg tcgtctggtg cgggttctgg attcggagaa tatatttgaa tcgggtctgg
    12601 atttaaacga aggtggttat catgaagttg tgtgggacgg actcgatgcc gacggaaatt
    12661 tcgttgccaa tggaatctat ttctacaaaa tttccgccaa atccggtaac aaaaccgtaa
    12721 ctaaaaccgg gaaattagcc aaagcgcgat gaaacgaatt ctgataattg ggataatact
    12781 gatcagcctg ggtagtggac tagttgctgg cgaatatgcc gatgcctttc tcgaattggg
    12841 agtaagtgcg cgagcttcgg ctatggctaa tgccatcgga gcgctggatt tttcggaaac
    12901 ggcttttata actaatccgg cagggatcag ttacatccgg caggcgcggc tggggctgat
    12961 gtacacttct caatttggat tggctgatca caactatatc ggtttagcag tgccggctgg
    13021 tgaccattat tcactggctt tgagctggat tcgctatgga gtggacgata ttccgattcg
    13081 gcctgatatt tttactacaa ttaccgataa agctgaacgg cgtgattcga ttattagtat
    13141 ttataacggc aaattgccaa ctttcaatga tgtggaaaat gcggtgtttg tctcgctggg
    13201 gcgatttaat tcgactatag ttgatttagg ttggcgctat tcgaaattca agctggaatt
    13261 tccgatcggg ataaatttaa aattcatcca taaaaaatat tacgaactcg aaggtttcgg
    13321 actgggagtt gatattggcg gacggttgcg ggttgatggc gaggagttgc tggatgtaag
    13381 taatttgggg atgttgagtg ttggaatggc tttgaaagac gtcactggta caaccattta
    13441 ctggaatact aagagtcagg atcgtattcg gttgaccccg gcgttgtcat ttgccttgga
    13501 acagccgatt aaaccattaa aattgattat aaatctagtt tctgaaaaac aatatcgtta
    13561 ccatgataaa accaattacg gcgttgaaat tatttacgct aatagtctgg cgctacgagc
    13621 tggcttaaaa aattctggca taagtctcgg attaggttta aattttaaag ttatgaagaa
    13681 actaattaac ttagattatt cattttatag tcatgatctc ggagcgagcc accgtgtcgg
    13741 gcttggaatt ggtatttagt aaaaatgacg acgtcgaacc aaattaattt aatggtggct
    13801 ttaattttac ttcttaatac ctgtggaaaa aacctggaag atccggatcc gccagctcaa
    13861 ccggaatggg tagcaaaatc cttaccgtat gatacgaccg agaccggcat tgatgccgtt
    13921 cctaatggtg attttatcca attagaatgg tataccggcg atgaggacga ccttagcagt
    13981 taccggattt atcgggctag cggagatatt gaaaataaat ttgatctgat agaagaaatc
    14041 caatctgact taatggcagg gagtgtcaat atctatctcg acgaaaatgt ttctattaat
    14101 accccatatt tttattttct gcgcgccgta gaccaggctg gaaatcttag tctgcgttcg
    14161 gatacggtag agtataagct agtggttaaa gtaaatttac gtggactatt tgatactact
    14221 gatgtgaacc caactttcaa atggtcggat gctgggtcgg ccgcttctga atatgtcatc
    14281 cgagtagaac agtttaatcc gaatcgggtg atgtgtttgt cagctacatc tcgacaaagt
    14341 tataccgatg aagttcagag tttaaaatat ggaaccggga ttattttcta ttcagctcaa
    14401 gatacgctaa gtcgaggagt tgattaccgc tggcgcgttg acgcagtggc gactttcaat
    14461 cgtgaaggcg atgaggtcgc cggttcggaa tccaattggg gatattttaa aattcaataa
    14521 taattaggag taattgtgaa aagagtttct ttatttatga ttttaacctt attaagcgtc
    14581 caactttatt ctcaggaaag tcagacgatt actactagat ccggtcgaac agccgatcgg
    14641 ttgtccgcat ccaagtatat tctgtcaact ggttcagacg ctatgttaat gactgtcaaa
    14701 atttggggcg aagtaaccag accaggcctt tacgacgtac caatcggcac cgatttgatt
    14761 gatttaattt cagccgctgg cggtccaact actcgggcaa atttatccaa agtcaaatta
    14821 atccacggtt caccggacga ggaagagaat tacgtaacta ttgttaatat caaaaaattt
    14881 ctggaaaccg gtgatgtgga tatgattcct gaaatcaaac cgtccgatac gattgtggtt
    14941 ccggtcaaga ttacgcaaca ttttctgacg tcattatcgt ggacgcaaca attattaagt
    15001 ttgttctcga tttatagtat gatactttat tacacaagta tagcaaatag gtaaaaccat
    15061 aggagtgata catgattgac agtattgacg tccaaatttt agatattttg cagaaggaag
    15121 gccgtattca gcgcaataaa attgcggagc aggtcaacct gaccattccc gctgttagcg
    15181 aaaggatgcg gaaattagaa aaaaaaggca ttatcgaagg ctatcacgct aaaattaata
    15241 gcgcggccat cggaaaggat gtgacggctt ttgtttttgt aatgacttcg ccatcgaaga
    15301 attatgatga atttgtacgt cggagtcgtg aagaaagtga tataatagaa tgtcatagta
    15361 tcaccggtga aggctcgcat ctcctgaaag tacagactga taatacctca gccctcgaaa
    15421 aattgctctc aaaaattcag tcctggccgg gtgtgttaca aactcgcacc tatatcgtac
    15481 tttcgagtta taaggacttt tcgtcgctga agccggcaag ctcgttagtt aagaagtaaa
    15541 ttttcctcgt gcaatttgcc gtttttatta ttaaggttaa aaatacttaa ttaaggtcga
    15601 atattaaaat gttgagtgat atccaagttg aattagataa tattgcgccc cgcattgatg
    15661 aattgcggag gtttctttga cgtcgagtcg aagaaaaatg aattgaatac cgagcgtcag
    15721 aaaacgaccg cctcggattt ttggtctgat ccagcccaag cgcaaacaac tcttcaaaaa
    15781 attcgggggc tggaaagcga aatcgataat tttgaaaatc tggtgaaata tcaaaaagaa
    15841 atcacagact tcgtggattt agccgctgag gaacaagatg actctgtctt ggctgaattg
    15901 gaaaagatgg taaacgaact tattagtaaa gtcgaacaat tagaattgga aacaatgctc
    15961 gatgaaccgg acgatgagaa gaatgctatt ttgacaatcc atccgggcgc cggcggaacc
    16021 gagtcacagg attgggcgca tatgctgatg cgtatgtatt tgcgctggat ggaacgcagt
    16081 gggatggagg cggacgtcat tagtattctc gatggtgaag aagctgggat caaggaagtt
    16141 actatcgaag tcaaaggtag atacgcctat ggctatctca aagccgaagc cggaattcat
    16201 cgcctggtgc gcatttcacc attcgacgcc aaccatcgtc gccatacttc cttcgcttcg
    16261 gtttttgtct atccggcaat cgaagacgat atatcggtcg aaatcaatca ggatgacctg
    16321 cggattgaca ccttccgcgc cagcggtgcc ggcggtcaac atgtcaataa aaccagttct
    16381 gcggttcgga ttactcatat tccgaccggt atcgttgtcc agtgtcaaaa tgagcgctct
    16441 cagtttaaaa ataaagatag cgcaatgaaa attctaagag cgcgcctcta tcaattaaaa
    16501 aaggaagaaa aaaataaaga aaagatggag atcgagaaga ataaaaaaga tatttcctgg
    16561 ggtaatcaga ttcgttccta cgtctttcag ccatacactt tagtcaagga tcatcgcacg
    16621 aagtacgaaa ccggcaatat tcaagccgtg atggacggcg agatagatga attcctgcgt
    16681 cagtatttat tattcaatgt caaaaatgaa agagctaaaa agtgaataaa gaagaacaag
    16741 caaccggaaa gtcattaaac gaactacttg cgattcgtcg cgaaaaactg caggcaatcc
    16801 gtgaaatgaa tttccgggcg tacgaatatg aatttcaaaa aacgcattcg gcggctgaaa
    16861 taacccagaa ttttgatgaa ttaactgaaa aagtagtagt aagagttgcc ggacggctta
    16921 tggcaatccg taaaatggga cgcgccagtt tcggccatat tcaagatgaa agcggacgaa
    16981 tccaaattta tgttcaggaa aacagcgtcg gtcagcaagg ttataagcta ttcaatttac
    17041 ttgacatcgg cgatattatc gggattaccg gtagagtctt tcggactcgc acgggcgaga
    17101 ttacaattct agttgaagag ttgcaattgt tggctaaaaa cctccgccca attccgattg
    17161 tcaaggaaaa agacggacaa attttcgatg cttttagcga caaagaacaa cgctaccgtc
    17221 agcgttatct cgatttgatg gtaaatccgg aagtgcggga ggtttttaaa actcgttcca
    17281 aaattattca atggacgcga gattttctca atgaacacgg ttttctggaa gtcgatacac
    17341 caatccttca gccgatatat ggcggtgcta atgcccgccc cttcaagact tattataatg
    17401 ctctcgagcg cgaattttac ttgcgaatag cggttgaact ctatcttaag cgcttaatca
    17461 ttggcggttt tgaaaaagtc tatgaaatcg gtaaaaattt ccggaacgag gggcttgacc
    17521 gcagtcataa tcccgaattt accttgcttg aattttatca aacttatgtt gattataatt
    17581 atatgatgga ttttgtcgaa cagtatttcc attatctttg ttcgaagctg ggggaatcta
    17641 aatttccttt cggtgaatac gaaattgact tcagtcaacc gtttagccgc cgaaaaatgt
    17701 tcgatttaat tcacgaatat gttggcgaag atattaccca atattcagaa gctgaattga
    17761 agagtttatg cctgaagaaa aacctcgagt tgaaaccagg cgacaattac ggtaaatata
    17821 ttgaattact gttctctaat ttcgttgaac cgcatttgat ccagccgact tatgtaattg
    17881 actatccgaa agccatttca ccactggcta aaaacaaacg cgacggttca ccaaatctcg
    17941 tcgaacgttt tgaactttat atcgccggtc acgaatttgt caatgctttc agtgaactga
    18001 atgatcccat tgatcagcgt gaacgtctgg aacagcagaa tcggctgagg gaatccggcg
    18061 acgaagaagc ccagacgatg gatgaagatt ttgttacggc gctggaatat ggaatgccgc
    18121 cgaccggtgg agtagggatc ggtattgacc gagtcgtaat gctatttact aatcaggttt
    18181 cgattaagga cgttattctt tttcctcaga tgcgtacctg agaaagtaaa gcgatgagcg
    18241 tacccttttt catcgccaag cgccatcttt tcagccgtca taaaatcggc tatatatctt
    18301 tcataagtat tatcgctacc attagcttgg cggtcggagt ggcggcttta attttgacga
    18361 tctcgatcct gaacggtttc gaacaggaaa tcaagaccaa attactcggg ttcgattccc
    18421 atatccgttt gcgtttactt tatcaggatt caatggactc gacgctggcg attcggtcag
    18481 agctggaaaa aatgccggag atcgagtgtt tcgtgcccta tattcacgga gccgcgatga
    18541 tccgcaaagg gcaggaaacc gaaggaataa ttgtcgaggg aattgacgaa gttcacattc
    18601 gacaaactct ggcggttgac cgctttatta aaaaaggcga actgcgtttt aatttgccgg
    18661 atggtcgcga tggtattgta attggtgcga aattagccag gcaactcggc gtagatattg
    18721 gtgataaggt ttacctcttc gtatttcaac cggaagagag ctacatcaaa cgaccgcgca
    18781 ttatgacttt tacggtaacc ggtctgtatg actcgggcat tgctgattac gatgacgttt
    18841 ttatctatac cagtttgcaa gcggcgcaga agctctatga tttgggcgag aaattttctg
    18901 gttatcaaat ccggctaaaa gatcagtcca aagtcgaagc cgtgaccgaa tatattaaca
    18961 aaactcttgg ctatccctat aatgcgctta gttggaatga tctacactcc aatcttttcg
    19021 aatggctgag gattcagcgc attccgataa tgctgatttt cggtctgatt gcggtcgtgg
    19081 ccgttttcaa tatcgtcagc tcaataatga tgattgtgat cgaaaaaact cgcgacatcg
    19141 gcatccttaa gagtgtggga atgaataatc gtcaggtgaa gcgcatattt ttatatgaag
    19201 ggctttttat cggagttgta ggaactttgg ctggatttgg gctgagtata ttattaggtt
    19261 atctccagaa ccggtttaaa ttaatttcca tcccggcgga tgtttatttt atgagttccc
    19321 tgccggtatt gttagagttg cggaattttt taataatcgg gttaattgcg atagcttcgg
    19381 cgctattagc cacgctttat ccgtcgtata aagccgttaa attgactccc agtgaggcga
    19441 cccgttatga ataagaattt tctgaactgg atcgctttac gttatttccg gtctaaaaag
    19501 aagaccggtt tgatttcttt tacgtcttat atctccatta tcggcatcgc actgggagcg
    19561 tttgcgatgc tggtggcttt gagtgtgtta aacggttttg aggcggaaat caccaataga
    19621 gtcatcgaca tcgaatcaca tctgcggatt agcggacgca atctccaatc agctcaaatt
    19681 gaacgggtta aagaagcgct atccggcgtg ccagtgcgcg agatttatcc atttgtaatg
    19741 aaaaagagta tattgtcttc ggaacaaatg gaagccgtgg tgcggattaa agccatcgag
    19801 tctgctacac tggaacaaca actttccggc ccgcggacta tgatgcgcgg tggggcaaat
    19861 ttcttatcgg caacttcgaa tctacccggc attgtggttg gttatcaatt agccgataaa
    19921 ctcggtgtct atccgggcga tactttaaat gtgattaatc caaccagcct gggaagcggc
    19981 ttgaatattc caattatcgg tcagtttgcc gttactggag tttttcaact ggacttattc
    20041 gattacgatg agaatattgc ttttatcgaa ttaaaagtcg gtcaggaaat attcgaaatg
    20101 ggagataatt acagcggaat tgacattcgt ttggaaaatt atcgctacgt cgagaaagcc
    20161 aagcaacaat tggttgctca gctcggtaac gaatatgatg tggcttcttg ggaagatttg
    20221 catcgtacct tgtttggagc gatgaaatta gaaaaatacg gcagttttct ggcgcttagt
    20281 ttcattattc tggtggcgat cctgaacttg accagtgcat tggtgatgct gattatggaa
    20341 aaaatccagg aaatcggtat gctccaagca ctgggtatga accagactca agttcgccag
    20401 atttttctta gactgggatt tttaatcggg agcatcggtt tgacggttgg aatagggctg
    20461 gcattaatcc tttgtctggc gcaacagcat tttcaattta taccgcttcc gtcggtatat
    20521 tatattcctt atctgccagt acaggtacaa tggagcgacg ttttgatgat catcggcgcc
    20581 ggtttgatct tcatctattg cggaacgctt tatcccagct ggcgagttgg taaacttatg
    20641 cctttggagg ctattcaata tgaaaaatga gaatgacaaa accttattga ccgtaaagaa
    20701 tctatcgaaa tcatactgga acgacggctt ggaaattgcc gtgctccgca atattaattt
    20761 aacggttaag gaaggtgaag ttgtggcaat cgttgggcca tccggcattg gtaaatcaac
    20821 tctgctgaat ctattaggga ctctcgaccg accggattcc ggcgtcatag aatatgaggg
    20881 gcttaatccg tttgatttat cagatacgga aattgcgcgc ttccgcaata ctcacattgg
    20941 atttgttttt caatttcatc accttttgcc ggaatttacg gctctcgaaa atgttatgat
    21001 tccggctatg atttataacc gagatgtcag aaaagcggaa gaaatggcac gtgaaatgct
    21061 tgcgcgagtc gggctcgctg aacgggctac ccatcggccg gcggcattgt caggtggtga
    21121 acgccagcga gtagccgtcg cccgggcgct aatcaataat cctttgatta ttttagcaga
    21181 tgaaccgact ggcaatctcg atgcgatgaa cagtgagcta ttgattgaat taatgttaga
    21241 attgaatcgg atatatcggc ggacttttat cattgctact cataatcaga caattgccga
    21301 aaaatcccac cgcattttga aattcaccaa ggattctctg cttatcgaag ccagcattta
    21361 actacccaaa taagttaggt ggttaattac tgaaaaatgc tacgccaaag tggcaaagat
    21421 tttgcacaac ataaaaaagg aaagaaaaga ttgacttagc agtcagagag ttttaacttc
    21481 tgatgttatt tgagaaagaa ttgaaataat gaaagagaat ttttccaaga taattcaaat
    21541 tattgtcaaa gccgcccgct atgaagcagg ccggatgaat catagttatg ttggctccga
    21601 acacttgctc tgggcgataa tcaatatgga gcgaagcgtt actcttgaga tattgatggc
    21661 gctcgatgtt gatatcaaag ccttacaaac tatgctgact gattcattaa aagcgatcgg
    21721 cggaacggcg acaatcggac agattccgtt tactaaacag gtggagcgga ttcttcgtaa
    21781 tacttataac gaagccaagg aactgggcgc cgaagtcgtt ggtgatgaac atattactct
    21841 atcaattttg aaagaaaagg agggaatttc ctacgaaatt ctcaatagtt ttggcgttga
    21901 ttattataat tttcgggctg aaatttcagg aaatatggat gagccacttc ccgttacaaa
    21961 aagtgagaaa aagagttcac gaacaccggc acttgaccac ttctctcggg atataacgga
    22021 aaaagcccgg actaaccaac ttgatccagt gattggtcgc gatcaagaaa ttgaacgagt
    22081 agctcagatt ttgtcacgtc gcaagaaaaa caatccagtg ctaatcggcg agcctggtgt
    22141 tggcaaaact gcaattgcgg agggattagc tctgcgaatt gtggctaaaa aagttccgca
    22201 tctacttgcg aacaagcgga tcattgattt ggatttggcc gcgatcgtcg ccggtaccaa
    22261 atatcgcggt caattcgaag aacgtatgaa aaccattatg caggaactcg agcaggcgcg
    22321 cgatgtgatt ctctttatag atgaactgca taccatagtt ggagcagggg gagcgtccgg
    22381 ttcgctggat gcctctaata tgttcaaacc ggctttagcg cgcggtgagt tacagtgtat
    22441 tggtgcgact acgctcgacg aatatcgcaa atttattgaa aaagatggcg cgttagaaag
    22501 acgtttccaa aaagtcataa ttaatcctcc aagtaaagaa gaatcgctgg aaatcctaaa
    22561 aggattacgg gagcgctatg aagcccatca tcacgtcaaa tataccgatg aagctttatg
    22621 gacggcggtc gaactcagtg accgttatat taccgataaa tttttacctg ataaagccat
    22681 tgatgttatg gacgaagccg gctcgcgggt tcatctacga gatgttatta tgccggagga
    22741 aatacttgag gctgaaaaga aactggaaga agtccgcaat cagaaagata acgccgttag
    22801 agaacagcag tttgaaaaag ccgctgacct gcgggacagc gaacggcggg ttaaaaaaca
    22861 attagccgat ttacagcaag cctgggaaaa aagtggcgaa aaacaaacct tattagttac
    22921 tgccgaggat attgctgatg tagtagcgat gatgaccggg attccggtta acagaattgc
    22981 cgaatcggaa aatcaaaaat tactgacgat ggaagatgaa ctccgcaagt caattatcgg
    23041 ccaggacgat gcaattcgag tcgtaagtag tgcgattcgc cgtgcccgtg ccggcttgaa
    23101 agatccgcgg cgcccaatcg gatctttctt atttttaggt ccaacaggcg ttggaaagac
    23161 cgaatttgcc agagtactgg ctcgcttctt atttcccggc acagatgctc tgattaaaat
    23221 tgatatgtcg gagtatatgg aaaaatttgc agtttcgcga ctaattgggg cgcctccggg
    23281 atatgtcggt tatgaagagg gtggtgaatt gactgaacgg gttcggcgtc aaccctattc
    23341 actggtgctt ttcgatgaga ttgaaaaagc ccatcgcgac gtttttaata ttttactgca
    23401 gattttcgat gaaggcgttt tgactgatag ttttggtcgt cgggttgatt ttaaaaatac
    23461 gatcataatt atgacttcca acgctggttc gcgcagtctc cagagctcgg cgtttggttt
    23521 ttccaaagcc gatccgctta gcgaatatga ggctatgaaa aaacgcttgc tggaagaaat
    23581 tcaacatgta ttcaatccgg aattcttaaa ccggattgat gatccgatcg tctttaaacc
    23641 cttaactcgt gaagacgttt tagcgattat tgatttgcaa ttggaggata ctcgccgcaa
    23701 tctaggagct tcagggacga ccttagaaat cactcaggaa gccaaagaac taattcttac
    23761 gcagggattt aagcccgaat ttggagcgcg tccactacgt cgggcaattc aaaatatgat
    23821 cgaggatccg atttcagaat tgattctggc tggcacttta agacgcggga gtaaaatcgt
    23881 ggttagtgtt actgataaca agatcgtctt tactcaacct gaaatagaat ttaaaatttc
    23941 ttccacgaaa attacgagcg aaccggtcag caattaagtc ataatttcag tcttttcaaa
    24001 ccaaatatag tatttagtaa gatagtctga cgttgtgtcc taataactat ttggcacgag
    24061 gtttgtcaat cttttagtga aatttgaaaa attgaagccc aaaaaaagga gataaatatg
    24121 ggaaaaatta taggtattga tttaggaaca accaattctt gtgtggctgt gatggaaggt
    24181 ggtaagccac gtgttcttga aaattccgaa ggacgtccgg tgacgccatc cgtggtagtt
    24241 tttaaaaaag atggcgaaat agtagttggt gatactgcca aacgtcaaat cgtaaccaac
    24301 cacgatcgcg ccgtttattc gattaaacgt tttatgggac gtttgtataa agaagttcaa
    24361 cgagaacgtg aagaagttcc gtttaaagta gttgaaggcc ctaatggaga tgctcgaata
    24421 cgaattggcg atcgggatta ttcaccacca gaaatcagtg caatgattct gcagaaaatg
    24481 aagcaaaccg ctgaagaaaa attaggcgaa aaaattaccg atgcggttat taccgtaccg
    24541 gcttacttca atgattctca gcggcaagct accaaagatg ccggtaaaat tgccggtttg
    24601 gaagtaaaac gtatcatcaa tgaaccgacg gcagccgctt tggcttatgg tcttgataag
    24661 aaaaaggaag aaaaaattgc cgtttatgat ttgggtggcg gtacatttga tatttccatc
    24721 ctagaaattg gagatggtgt ttttgaagta aaatccacta atggcgacac ccacttgggt
    24781 ggcgatgatt tcgatcagcg tattattgag tggttgatta gtgaatttaa aaagaaagaa
    24841 gggattgatc ttaaaaaaga tccgatggca atgcagagac ttaaagaagc cgctgagaaa
    24901 gctaaaattg aactttcttc aaatctggaa acggaaatca atttacccta cattacagct
    24961 ggttctgctg gtcctatcca tctaatggaa aaattgtcac gagcgaaatt tgaatctctc
    25021 gttgatgatt tgattgaacg cactcgtaag ccgtgtcagc aagcattgga agatgccgga
    25081 ttaaaaccgg aagatataaa tgaggtaatc ctagtaggcg gttccactcg catacccaaa
    25141 gtgcaggatc tagtcaaaga cctttttaag aaggaaccta ataagagcgt caatccggat
    25201 gaagtagttg caatcggagc ggcaattcag ggtggtgttt tagcaggaga agttcacgat
    25261 attctactgc tggacgtaac gcccttgtca ctgggaattg aaacactggg tggagttacg
    25321 actaaattga ttgagcgtaa tacgaccatt ccgacccgaa agagtgaaat tttctcgact
    25381 gccgcagata atcaaaccag cgtagaaatc aacgttctcc agggtgagcg ggcaatggcg
    25441 gccgataaca agagcattgg gcgcttccat ctcgatggta ttccaccagc accacgtggt
    25501 gttcctcaaa ttgaggtcac ttttgacatt gatgccaatg gtattctgaa cgttaccgct
    25561 aaagataaag ctaccggcaa agaacaaagc gtccgaattc aaacttccag tggtttaacc
    25621 gaggaagaga aagaaaaaat ggtacgcgat gctcaactgc atgaagaaga agatcgccgt
    25681 cgcaaagaag aagtcgatac tcgcaatcag gttgatgcct tgatttacca gacagaaaag
    25741 aatatgaaag aatacggcga taaactgcaa ccggaagaaa aaggcagact gaacaacgca
    25801 ctggaagact tgaaaaaagc ccagaatagt ggtgatgtaa aaacgatgaa gagtgcgatc
    25861 gaagccatga atcaagcctg gtcagaagta tcatctaaga tgtacgaaag agtacgcacg
    25921 gaaacaccac caccacatac tggtcagact tatgaacaac cacatgagga aacgaccggt
    25981 caaaaagatg aaaacattca agatgctgat tttgaagtag tggacgacga taagaagtaa
    26041 atgaattcgt aaatggtaaa aagggcggct ttccgccctt ttttattatc agcccttgaa
    26101 actggaaaag gttttgtttt tttgagtaat aaaatctaat tttcaccgct ttttttaatt
    26161 ggcgacgtac ccaagtggct aagggagcgg tctgcaaaac cgttatgcac cggttcaagt
    26221 ccggtcgtcg cctctgattt gagcagtttt ttcatatagt tcattttgcc gaagtggcgg
    26281 aaatggtaga cgcaagggac ttaaaatccc tcgatccttt ggatcgtgcc ggttcgattc
    26341 cggccttcgg cacaactttc ataataagag actaagagtc tccttttatt ttaaaagtta
    26401 gaatagaatg atatctcaaa cactaccatt tgtatctacg attaatcatc ctgaatttga
    26461 atttgaattt aattattgat tttacacata aagtaaagta aaatacatat tatttgaaat
    26521 ttaagagaat gaggatcaag cgatcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26641 nnnnnntaac acgatctcgc aggccttttt ttatgtttac tgctggatca taaccacagc
    26701 tcagaaggag ttaaaaaatg gaatcatcta aaaaacaatc ggtaatcaac gaaaaaccca
    26761 ctcctatcaa aggactaacg acagaggaag cgctttctcg gctaaaaaaa tatggtccta
    26821 atacgttccc aagaagtaaa ccaccctcag cttggtcaat cctaatttct caatttaaaa
    26881 gcccccttat ttatataatc ctggtagccg ctgtgatttc actcattatg ggcgaactga
    26941 ctgatttcat tataattata gccgtcgtta ttgttgatgt cattctcgga tttttccagg
    27001 aatttcaggc tcaacgtact tatacggcat tgaataaatt gctaaaaccg accactactg
    27061 ttatccgcga tggagaacgc caggagattg atgtaacaaa agtagtaccg ggagatatcg
    27121 ttatactaaa tgcaggagag cgtattcccg gtgacggtga aattattgag ggcgccaggc
    27181 tcagtgtaga cgaggcaatc ctgaccggcg agagtgagcc gattaaaaag tcagtcgaag
    27241 ccggctctaa ccaggttttc atgggtacaa cactccttac tggtagagga gtaatgcgag
    27301 tcacccaaac cggtgttcga accgagttag gcaaaattgc caaaagcctt caggaacata
    27361 aagaagaaga gactccgtta caagtgcgac ttaaatcttt cagcaggacg ttgacaatta
    27421 tcgtcgtttc gttcactact attatatttc tcgtcggact tttactgaag cgtgattttc
    27481 tggatatgct acggacttcg gttattttgg ctatttcggc aataccggag ggcttgttga
    27541 ttgccgttac tgttattttg gtattgggga tgcgtaaaat ccttaagcgc caaggcttag
    27601 ttaagaaact gttagccgtt gaaactttag gttctgtcaa tgtcatctgt accgataaga
    27661 cgggtactct taccgaaggc cgaatgcgtg tgactcgtac cgattttgtg gataacgagc
    27721 gagccatcca gattatggtt ctgtgtaata accttgaggg accggttgaa atggctttgt
    27781 gggagtatgc caaatctcga ctgccgaatg atccccagac tttagttgaa gagagccaac
    27841 gtctggatga agaaatattc accagtgaaa ccaaatttat gattaccggt caccacttac
    27901 ctgatggcga ttatcatttc ctgaagggtg cgcccgaaat tgtcctgaat atgtgtcaaa
    27961 tcaacgatgg tgaacgtcag cgaattttgg ctcaaattga tcaatgggct ggtgaaggat
    28021 tgcgtcttct cggtctggct taccgtgact atggaatcct ggaagatcac accggttata
    28081 cctgggtagg cttgatcggt atggaagacc cgattcgcga gggagtgcac gaggcggttc
    28141 tgctcgctaa acatgcaggt attcaggtta aaatgataac cggcgattat cgccgcactg
    28201 ccgaacgtat tgccagtaat atcggtctat tgcaaaacgg tgaggaaaca ctggaaggcg
    28261 aagaacttgc tcaattaaat gacgaggaac ttgctaaacg tatataccct accgccgttt
    28321 ttgcccggat taggcctcat gataaattac ggatagttaa cgctttgcag aaacacggcg
    28381 atattacggc tatgatcggg gatggcgtca atgacgcacc ggcacttaaa caggctaata
    28441 tcggcgtggt tgtcggcaca gccagcgatg tggctaaaga gaccgccgat ttgattcttc
    28501 tcgataacaa ttttcgcacc atcgttgccg caattgagga agggcgcgta atctttgaga
    28561 acatccgcaa agtcgtagcc tatgtcctct ccaattcttt cgccgaggta ttgactattt
    28621 ttatatctat gattttgggc tggccggcac cattactggt tgcgcagata ctctggattc
    28681 acttgatctg tgatggtccg gctgatatcg tgctcggttt tgagccgaag gagaatggca
    28741 ttatggacga aaagccaaaa tctcttaaag aaccaatttt gcctccttta ggcttgtttt
    28801 taattggtgt gattagtatc ggcagtgcgc tagcggctct cctgattttt ggccactatt
    28861 ttaaaataca cggtgatgct gacgaaggcc gtagtttagt cttcgctagc ctggcgataa
    28921 attcactaat ttatattttc gcttaccgaa gtatgcgcaa gtcacttttt aaaatgaact
    28981 cgatcctgaa aaacaaaccg ttaatctttg ccgtaatttt tggaatggtg ctggtttttc
    29041 tgccttttgt aatacccagt ttaagccaag ctttgggcgt tgtacccctg gacctgaaac
    29101 aatggttatt gatcttcacg atagcattaa tgcttcttat tgcggtggaa attggcaagg
    29161 cagtcagcaa ctggtggcac aataaaaaaa atactctgac taaaggataa aaatgtttat
    29221 cctaatagaa aatgcaacta aaactcgttt ttgggtctaa tctcagcaat attgtttata
    29281 tttgcttact taatttgata ttttaaatta ctttgtttat tttggaatta gtcaattgat
    29341 tgttgaaagc aactatggcc aaaattgatc caaactccga ttactattct gaactctccc
    29401 agtttacgat cgagagcgat gtggatgcta ttctttggat cgatgctgag agtacgattt
    29461 accgggtgaa tcaagccgct tgtcaattac tgcaatactc gccagaagaa ttggtcggta
    29521 aaaatttaga tgatctaatt gacattcaga atgattggaa taaaaccgac ccacaccgct
    29581 ttttagacca gattgatatc ataaaacaaa atggtgtcac agttatcgag cgcaatttcc
    29641 gtacgaaaga cggtcggatt ttgactttgg aaatctcagc gcatttattt gttttcaaaa
    29701 ataatgaata tatctgcgtg tttgcccggg atataactga acggaggaaa gcccaggaag
    29761 agctgcaaaa gacactgaag gaattggaat ttttaaaaaa taaattatat gaagaaaata
    29821 tttaccttca gaatgaaatt aaactaactc acaactttga agaaattgtc ggcaaaagtc
    29881 tgagtctcag aaaagttctg gaaaaagtgg aacaggtagc cgcaaccgac gctaccgttt
    29941 taatacaagg cgagacagga accgggaaag aacttattgc ccgcactatt catcatatta
    30001 gttctcgtag ttccagtcca ttagtcaagg taaattgtgc tactttacca gccgccttaa
    30061 tcgaaagcga attattcggg catgagaaag gagcttttac cggggcactt tatcgtaaaa
    30121 tgggacgttt tgaactggcg aacggcggta caatttttct ggatgaaatt ggtgatttac
    30181 cactggaaat tcaatctaaa cttttacgag taattcagga aggtgaattt gaacggattg
    30241 gaagtaacaa gaccctcaaa accgacgtac gtataatagc agccactaat cgtaatcttg
    30301 aagccgctgt ccaaaaaggt gagtttcgta aagaccttta ttatcggttg aatgtttttc
    30361 ccattcatgt gccgcctctg cgcgaaaggc cagaagatat tcctctctta atacatcatt
    30421 ttgtccaaaa atattcgaag aaaatcggtc gcaaaataga tattatacca gctaaaataa
    30481 tgcgtacttt aacaagctat agttggccag gaaatatccg ggaactagaa aatattatcg
    30541 aaagagccgt tatcattacc aacgggcgaa ctcttcagat tggtaattgg tttacaactg
    30601 atgcgccagt gatgaataag gagaaaattc aatccttacg agaagccgaa agggactata
    30661 tcttgaaagt actatctttg acaaactggc gagttagcgg gcctcacggt gcggctcaac
    30721 tcctggatat caatccccaa acgttagtct ccaagatgaa gaaattgggc attcatcgca
    30781 ctaaaccaat taatgaataa taataaattt tatgagatat cataaaataa tcaattgcta
    30841 aacccagcaa attttgatcg aattattaca ttattcttta attaatagac tgttatcaaa
    30901 caaagtcaag gatttggtat agattttgtc cttaataaaa ttgtatgaag gtaaaaaaag
    30961 aaaataagag tattaaaaaa gaagatccga aaatcatcga tatgtcaact tttgatgagt
    31021 tgattcatag caacaaaccg gtggtggtcg aattttacga tgaaaaaatt agcacgagtt
    31081 atatagttga ttcgatattg tcccggctcg ccaactattt tcagaaccag attgaatttt
    31141 tgaggcttca tataaatcaa catcgagcaa ttgctgataa atatcatatt ttttcatcac
    31201 cagccacttt aatcttttta aacggtaaac taatcgaggt tatcctcggg actttttcgc
    31261 agaaccagat aatcgagatt ctcaaccgtc ttctttagta aaaaaaggag gttaataata
    31321 tgacgacttt taagaaatat gtcaataaaa ttgcactcga caaattagta acctactgta
    31381 tcaaagaccc gcagaagcat attcctaaac tcattgattt tgttgataaa agtgcctggc
    31441 ttccaattca taaaagctat gtacaaggag caaaaagcat tgtcaataat cccgattcca
    31501 actggtatca actagctatg cgagcaatta gggatataga tccggatatt atccataaaa
    31561 tagtgagaaa tctgtttatt aattctggct tcgagggaca gggtaagtta gttgaagaga
    31621 gggagaggct cggttttggt attccctggg cggtgctaat ggacccaacc agtgcttgta
    31681 acttgaagtg taaaggttgc tgggctagtg agtatgacaa gagtctcaat cttgatactg
    31741 atctaatgga tcgaataatt cgggaaggta aagaacttgg tatttacttc tatatctaca
    31801 gtggtggtga accgctgata cgtaaaaacg atttgattaa tctagctgaa aagcactcgg
    31861 attgcgtctt tctatctttt acgaatggca cattagtgga cgaacacttt gcggatgaat
    31921 tacgaagagt aggaaatttt tcgctggcat taagcattga aggtgatgaa aaagcaaccg
    31981 atttccgaag aggcaagggc agttaccaga agctgatttc tgcaatgaaa attttgaaat
    32041 cgagaggaat tccttttggc ttttcgacct gctatcatag ccagaactgg aatatcgtgg
    32101 gggaaccgga atatcttgat cagatgattg aagccggatg ttattttggc tggtacttta
    32161 cttatattcc acttgggaaa cacgctgatc ttagtttgct tgcccgtccg gaacatcggg
    32221 aacatatgta ctaccgtgtg cgtgaggcgc gccagactaa accaattttc ttgatagatt
    32281 tctggaatga cggggaatat gtcaacggtt gtattgccgg tgggcgtcgt tatttgcata
    32341 ttaacgccaa tggcgatgtc gaaccttgtg cctttattca ttattcggat accaacatta
    32401 agaatgtatc tttattagag gctttacaat ctccactttt tctccaatat cagaagaatc
    32461 aaccgtttaa tcaaaatcac ctgagaccat gtccattgct ggataatcct gacgctttaa
    32521 aatcaattgt actggagact ggggcatatt cgactcaaca tatggatcgt gaggatgtgg
    32581 tagaattaac tgctaaatgt caggatatag cgcaagcctg gaaacttaca gccgatagat
    32641 tatgggaaga gactctaaaa aatcgagtcc aaaagaagtc cggagtgaaa caatatgagc
    32701 gggaactcgt tgatgcagaa atccataatt gatataaatg aaaagagaca ttctgtggca
    32761 agcgatttag gcatactggg aatatggctt ttcatcagtg aactaatcgg tggtacttta
    32821 gtaaatagat taattttaat aattggcgga attgattgtt atttcacaac tatatactca
    32881 taaataaaaa gtaagaattt tttcctcccg agactcaata gtctctcttc ctcccaaaaa
    32941 gcggtcggtt atctctccgg ccgctttttt tataacgact aattaagaaa aaccagaatg
    33001 agatgaagga tttaatattt tcttccgtct tcattattct ttctaaaagg tattaaacct
    33061 actgttatga tattgattat ttaacaattt aaaaatcaca gcgaatgtct tcttagaaaa
    33121 aaggttgaca ttattctcta tcctctttat ccgatgagta gaagagaatt tagtttgagt
    33181 acgccagtaa gaatttgaac tgaaaataca atcagaaatt gagatgtaac cacggctaaa
    33241 atttttattg gtaaattaaa aagtgttaaa tatatttgaa acggttcaat gaataattca
    33301 aatttcatat tcaacgttaa cattatgctc gaaaaattag aattattaaa agattgcata
    33361 gttaaaaatt cctctctgtt aatgataaca ttatgagagt atttccggtt atttctgaac
    33421 gtaatcatca gcaatatttc cgaatatcca attctaagga ataggactta tgacagctaa
    33481 tagccaagcg gaaaacctca gccatttaac cttaaaagaa gcttttaaag atgatttcaa
    33541 aatcggggcg gctcttgatt tgaatcaaat ttccggtcgt gatgagcaag ggcttgccat
    33601 tgtcgaaaag cattttaata gcattacgcc tgaaaatata ttaaagtggg aagaagtcca
    33661 tccggaaccg gaccgatata atttcgatgc ggcggatcaa ttcgttgcct ttggtgagaa
    33721 gcataatatg caaatcattg gtcactgttt agtctggttt ttccagacgc cggattgggt
    33781 ttttgaggat ggggcgggca agccattaag ccgtgcagca ttattagcac gtatgaaaga
    33841 acacattttt acggtaatgg ggcgttataa aggccacatt cacggctggg atgtggtcaa
    33901 tgaagcgctt atgatagatg ggactttgcg aaagtgtaaa tggctcgata ttattggtga
    33961 agactatatt ctcaaggcat ttgaatatgc ccatgaagct gatcctgagg ccgaacttta
    34021 ctacaatgaa tacgataccg aacataaatc caaacgtgag gcactcgttc gactaattca
    34081 ggatttgcag gcgaaaggcg cccgcgtgga tggaattggt actcagggtc attggtttct
    34141 ggattatcct tctcgagagg aaatcgagaa ggcgatctta gagatctcca caactaatgt
    34201 caaacaaatg ataacggagc tggatgtcgg tgtgcttcct ttttatccgg ttgattccca
    34261 gatggtggat atcagttcgt tcgacccgga aatgcagaaa aagtataatc cttatccgga
    34321 cggattacct gattctgttc aaaaagaatt agcaaggcgt tatgcggaac tctttaccat
    34381 ttttcttaag catagtgata aaattgaccg ggtgacattt tgggcggttc acgacaaaca
    34441 atcgtggcgt agttacttgc cgattagagg aagggtggaa tatccgatgt tgtttgaccg
    34501 acaatgccga cctaaaccgg ctctggaagc aattttaaaa gttgctcaga atcgagctaa
    34561 attagtggag aattaattgt tgaaagataa gagcgaaatg gagaatcggt tattctgtaa
    34621 tcaggatgcg atccgtcgct ataacgttta attatttcag gaagaattac aaatggaaaa
    34681 gaaaaaaaat cttgaaattg tcgccgccgg gcatacttgc cttgatttga tcccagcctt
    34741 tacaattgag ggagaagttg aaaagatgac agatgtcctt gtccccggca aaatgatcaa
    34801 tatgggtgaa tgtgtgattg ccggtggcgg gccggttact aatgccgggg tatcaatccg
    34861 gcgtctggga gtgaaaaccg aactaattgg aaaagttgga aatgacgatt tcggccgtct
    34921 tgttttagat tggtacgaga aaaatgaagg tcattttaaa ggtattaaaa tggttgatgg
    34981 cgaatcaact tcctatacca tcgcaatctg cattcccggc attgaccgtt tttatttgca
    35041 tcattgcggg gctaatgata cctttggtta tgacgatatg gattttaatt tggtgggacg
    35101 tgccaaatta atgcttttcg gctatccacc gtggatgcgt aaaatatacg aaaacaccgg
    35161 cgctgagcta actaaaatcc ttaaaaaaac caaagagttg ggaactacaa cggctctcga
    35221 cctttctctg ccagatgttg acagttatgc cggacaggtt gactggaaag ctattttaac
    35281 tgactgggta ccgttatcgg atattatggt gccgagtgcg gaggaattgt tctattttct
    35341 atataaagat aaattcttgg aaaaaaggga caaacttaga cataaggaaa ccgttctcga
    35401 ccaaatgacc gttaaggaaa tttctacgat gggcagggac ctgataagaa tgggagcggc
    35461 gatcgcaatg ataaagtgcg gtagtcgcgg cctttatatt tacactgccg gccaagaacg
    35521 cttgaagaag atgggcgcgg cgggatgtgc cgaccttgag aattgggcta atcgcgaact
    35581 ctggtttccg gtttatcagg aagaaaagtt cgttggcgcg ctgggctccg gtgattcggc
    35641 tatcgccgga tttctctcag cctttgtctg ggggcatagc atcgaatctt gtttgcgcta
    35701 tgctaatgcc gccggtagta tgaatgttac ggtgcctgac ggtttaagct ggaataaagg
    35761 atttgatgat ttgaccagac gcataaaagc cggatggaaa accaaagaga tgaaaataaa
    35821 cgaaccgggc tggaaaaaag aacgcgagtt ctgggttggt ccggataatc gtggtaaatg
    35881 ggaatcataa aataatagga gaataaaatg tcgaaagaga gagatgtatt tattcagaaa
    35941 aaaatagccg gaatgacttt aatggaaaaa atcgggcagt tgctgacttt cacgcgcaga
    36001 ggaacgcttc tgaccccgag tggaatcgag cagattactc gtttgcattg cggcggtctg
    36061 tgtctcgaac cgtatgcagt cgagacttgc aaaaacctgt attggggcaa ttcccagata
    36121 gataagaatt ttcaaccacc gaaagattat tttaccatcg ctaataatta ttttgatgga
    36181 aaaaccttcg gcatcagcat tacgccggaa gattatacca aagacttaaa ccgcttgcag
    36241 aaaattgcta tgagccggcc ttcgggtatt cctctccata tgaccattga ttttgaaggt
    36301 gattttaaaa atgattatat gtccggcggt attcgtcagt ttcccggtcc gatgggaatg
    36361 gcggctatcg gtgatgagaa attgacttat gaaatcggaa aaaccattgc cagaatgtta
    36421 acagcaattg gcgtaaccca gatgtactcg ccggtctgtg atgtcaatac aaatccaaga
    36481 aatccagaga ttggcgtgcg ctcttttagt gatgatcctg aagtttgcgc cagacatgcc
    36541 gtcgcgttaa taagaggatt gcaagattac ggtattgcgg caaccgccaa acattttcct
    36601 ggccgaggtg attcaggttc ggatgctcat gatgtgctgg aagtgctaaa tctcgacaag
    36661 aaaagaatgc acgaagttga gttagtgcct ttcaaagcgg ctattaatgc cggagtcaag
    36721 gcgataatga ccggccattc cgtctacccg gcctttgacg acaaatatcc tactaccttg
    36781 tcagaaaaaa tcctgactgg tctcctgcgg gaagagctgg gattcgaagg cgtgattgta
    36841 tccgatgcta tcggtatggc ggcaatttta aagcagtggc ctttaccaca agcctgcgca
    36901 atggcgatca aagccggctg tgataccatt ctcttaaaag ccgatgatga gtctcgctca
    36961 cagtgtttct tcgggattaa gatggccgtc gagcgaggcg aactgagtga agagagaatt
    37021 gatcagtcag tcgcgcgtct cctgaaaatg aaatatgatc aaggattgtt tgaatccgcc
    37081 ggagaaaaag accctgagat aactcgggaa attaccaccg ctaaagaagt aattgatttc
    37141 tcttgggatg tggctaaaaa agccttaatc gtaatgcgcg atgagcaaca ccttttgcca
    37201 ttggaccccc agcagagaat tttagtcatt gagcaacgca ttccgtatga gtttcttggc
    37261 aaagaccctt attgccatac gcatatgttt tgcgaagcaa tggtcaacca cagtcagaat
    37321 ttgattttgg cggatacagc cttttcggct tatgaagatg agattaagga gtgcctcgaa
    37381 ttagccaaac aggctgacct ggtcgttatg actaattact atgcccgcat tgtaaaatcc
    37441 ggtaataatc ggcttttagt caagaaattg aaggaagccg gacataaagt cattgtcgtt
    37501 actaataatc cttacatcga aggtactaca gtggatgccg atgccgtcgt ctgcaatttc
    37561 agcggaacgc cagattcgat tcgagtttcg acttacctgc tgtacggcaa agtcataccc
    37621 tgtccgacta ctaaattgcc cataaagcct gatgtcgggg agaaaacccc agttaaggat
    37681 ctcaaggcac ctaaaaaaca tccgctgaat ctttcttatt gttagatgaa gagatctgca
    37741 aggggatgga ctctatgaag tatggcaaat tatggaattt tttatatggt attgaataag
    37801 aaattactaa tgccacagac aggtaattat tgtatctttt aattataaat cgggaagaat
    37861 agcatggaaa aaattaagat tagggacgtc ttcactattt ccggaagaca aatatctaag
    37921 atttacaaag ataacaaaat gacagtacac tttcagggaa tgtccaagac tctgcaattt
    37981 ccgcagacca ttgctctgga aggtgctccg acttgttcaa taggtatttc tccggagggt
    38041 ttatataacc gccttaatga tgctgggcga ttaattttgc taaatcaaaa tgccatccaa
    38101 tttggttcag ccccgtttat taaaatcgcg gctgacccaa atgctgaaga cgaacgcgac
    38161 gaatatttca tgctaactac cagagatcct aatatcgtag aaggccccga tgatgtaccg
    38221 gcattaaaaa attactatca tccgccagtt cccgaagcaa aagtaaccat ttcactggct
    38281 actcccttgg cagagattat ttatgatttg gcgaaaataa gtgtaactcg ccttattacc
    38341 tcgcctttat tttccggaaa tgaccagacc cttattccta ttagcgtaga agaatttgtg
    38401 gtcgagaata cttccgataa aactcaagaa gtgaccctag taataccgcg tccctcactg
    38461 gtaaatcttc aggaaaaaga actaaagcca accgatcaag atacggtata tatttgctcg
    38521 gcttcagtca aagggcaggt gcatgaagcc tttaattcag ccggaatcaa aggtgtcgtc
    38581 atgggcaatc aagagtgcca ggaaaaaatg gcgatcgctg ttcctgaaat agagggtgtt
    38641 gatattgata ctcacccgca tttctgcctt aatcatttga aacaggatct gttgcttaac
    38701 gacgatggca gtttttatga aaaacgtaat gtaattccgc atcaggatta tggcgcggcg
    38761 attagtttac attttactct gggacctaaa gccagtctaa aaattccctt tgcaattgct
    38821 ctggattttc cccaacagcg ttttatcgat ggggtagaat ttgagcggaa gtatgtcaaa
    38881 ttattcaaga atcaagccag tcggacaata gagatgataa aagttgctct ggaaaactac
    38941 cctgagtggc tgaatcgaac attgaccatc caaaaccgga tttacgagct aattcgaaaa
    39001 aatccagcct atagagatga cgaagaaggt gctttgcgat tagcccgcct gatttttaat
    39061 gaatttagtt gcccgatatc gaacgcctgt gtttgggtta aagataatga aggcaaagag
    39121 agagctcgat tcctcgaatg ctttgattat gcttatatca atccttcgga cgtagactgg
    39181 tattcgatgg tgttgctatt tctcttccct aagattgagg aagaaatttg tcaaggcttc
    39241 atcaactcaa tcctggcgga agatccgacc ccgcgatttt accatttgca tgcttctttt
    39301 gttgaagcac gtcagcattt tgaaaaatac ccagaagagt acgaagacat ttcactaact
    39361 cagatttggg acgccaccaa agtaaaaggt agtgtagcgc acgatctggg tgctttgccg
    39421 aaagggcacg cgctccgcaa tgtcagtgat tacacctggt ataataataa ttactggata
    39481 gatcttttcc ctaaactggc aacgcgcgtc ctgcgcaatg tcaaattcac cggcaatact
    39541 gcttttgtaa aggagaattg ggaaactctt aaattcggtt ttgatcattt gcaagagctt
    39601 gatttcgatg gagacggtat cccggaaggg tatcccggcg aagtcaaaaa tacttttgat
    39661 aatttgacgt tatttggtgt agatgcctac gatgcgacca tttttatggc aggttgttat
    39721 gcaatgatca aaatggctga gattgtaaaa gataccgcgg caaaaaagca gtatgaagag
    39781 atttttgata aagtttctaa agaatttgaa aaattatgga gagacacgaa aaacagccaa
    39841 ggcaaaagac ttcaatacta tgtcacctgt tttgatccca agacgggaga aaccaatact
    39901 gatgtctgga tgaatcagtt ggatgccatc tgggcattga ttgctatggg ggaagagtct
    39961 tttatacccg aagagcgcat taagcaaatt cttaaaacag tctacgataa taatcgtgcc
    40021 tatatgggct gggcaatgtg tcgaacagaa gacggcggaa aagttgaatc ggaacaggga
    40081 caggatgtct atactacttc caattatgta tttgcacaac tgctagatta ttatggtatg
    40141 acggaggaaa gtaaggaagt atataaggcg atggacaagg tgatctttgg acatggaaat
    40201 accctaatat cacctgataa tttgcgtgcg gagctggaac aagaagcagg cgaatcagca
    40261 cctggtccgc attatatcgt tgccgcttat ccgcgtcctg gggcaattct gactcaaata
    40321 gtgctgcagt acattaaaga gacgcagaag caaacgaaaa atatgcttgt ggattctgac
    40381 aaattgaaga gttttgcgat ttccttaatg aaataaaagc tttgattaaa gcggaaaaat
    40441 cacgaacgtt attaaaattc taaaaactga gggtgcatta tgaaagaaaa aaccggagtg
    40501 ctttcactga aagaaaaaat cggctacagt ttaggtgata cggcttcaaa tctttttttc
    40561 cagactttta tcttcttttt gctttacttt tatactgatg tatttggtat tcctgcctca
    40621 tccgttgcga caatcttttt aattggtcga atttgggatg cggtcaatga tccactaatg
    40681 ggggcgattg ctgatcgaac taataccaaa atgggtaaat tccgaccgta tttgctttat
    40741 accgccttgc cctttggtat tttgggcttt attatgttca ttaccccgga tttaagcgtt
    40801 caaggaaaag tgatttacgc ttttattacc tataatttga tgatgatggt ctatacagta
    40861 atcaatgtac cttattcggc tctaatgggt gtaatatcac cccattcaga ggaacggacc
    40921 gttgtctcaa ctttccgttt tgtcgccgct tttgtaggcc agttcatcgt ccagtttgcc
    40981 gtcctaagat tagtgtctgt ttttggaaaa ggaaatgaac aaatcggttg gcaatggact
    41041 atggcggtga tggctggctt gtcggttgtt ctttggttag tgaccttcgc cacgaccaaa
    41101 gagcgtgtca aaccggtcaa ggggcaaaag aacccgctga agcaggattt ggctgacctt
    41161 ttgagaaacg tgccctggct actgattggc tcagcgacta tttttcaact actgttcaac
    41221 gtgattcgcg gcggcagtat tatgtattat ttcaagtact tcgtccagga tcagcagtta
    41281 tttttatttg gtaaaccaca tagctactcc taccagactt tgacctcaac ttttatgctt
    41341 actggtacct taatcacaat tgttgggtcc ttattgacca gttttttcag taaaaggatt
    41401 ggaaaatcga gaagttattg gggctttctt ggaatttcgg ctatctgctc agctcttttc
    41461 tttttcctta agccgcagga tgtggtctgg atgtttttat tgcaaataat tgcttcattt
    41521 tgtatgggtc cggtttcggt tctacaatgg gcgatttata ccgatacggc agattattcg
    41581 gaatggagaa cggggcgtcg cgctacggca cttattatgg cggtttcact atttgctctg
    41641 aaacttggca tagccctcgg tggctccatt ttagcctgga ttctggatat tta
//
LOCUS       DAOOMU010000007        36810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_892_length_36810_cov_18.416406, whole genome shotgun sequence.
ACCESSION   DAOOMU010000007 DAOOMU010000000
VERSION     DAOOMU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 36810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36810
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_892_length_36810_cov_18.416406"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            463..1374
                     /locus_tag="PLI55_01310"
     CDS             463..1374
                     /locus_tag="PLI55_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99508.1"
                     /translation="MKKKSKKYNPLRMEKELSDLIEYIENAGFESKEEIDRFLAKMGG
                     KKIGSVLNKKKTAADEAFELILEAQEVSPEEGIKLAKKAITLDPNCADAYTFIADHTE
                     DEDEALELYQKAMQVARESLGENAFIEYVGHFWGITKTRPYMRAKAGYASSLILKGDL
                     QSAIKEFQEMLVLNPSDNQGIRFSLSVLLLQLNRLREYEDLLKQYKDENSAGWLYNRV
                     LYLYLKYGGTTRTISALEEAIAENKYVMDLITGLEEPPKELPESYGIGSYEEAVLYYW
                     ESILTWMAHKKAILWLIRYWERRREIN"
     gene            complement(2638..3288)
                     /gene="phoU"
                     /locus_tag="PLI55_01315"
     CDS             complement(2638..3288)
                     /gene="phoU"
                     /locus_tag="PLI55_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPA99509.1"
                     /translation="MLTKKIQELKQAIVEYAALVESMITRSLQALSAKDVNTLKEIIN
                     VDESKANEFDLRMDEACIQTIAQYQPRARDLRTIIMILKMSNDLERLGDGAVHICRDA
                     LYLIDRPSFKPIPLLNHLAQESLGMLHDSIDAFINNDAESAQEICKRDDVVDDLRDKI
                     IAEMTQLMSKQPATVPIALRVMDISRKLERAADLSTNICEDIIFIVQGRVIKHGKN"
     gene            complement(3312..4079)
                     /gene="pstB"
                     /locus_tag="PLI55_01320"
     CDS             complement(3312..4079)
                     /gene="pstB"
                     /locus_tag="PLI55_01320"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPA99510.1"
                     /translation="MNLSKEIRIQAENVNFYYGDKQTLKNVNMSIFQNRVTAIIGPSG
                     CGKSTFIRLLNRMNDLIPNTRVEGRILLDNHNIYDPGVDVVTLRRKVGMVFQKPNPFP
                     KSIRDNLSYGLKINGIKSTDSIENTIRRSLEEAALWDEVKDRLNESALRLSGGQQQRL
                     CIARCLAVEPEVILFDEPCASLDPISTKRIEELIEQLKQRYTIVIVTHNMQQAARVSD
                     YTAFMYLGELIEFGPTEKIFTIPENKHTEDYISGKFG"
     gene            complement(4066..4920)
                     /gene="pstA"
                     /locus_tag="PLI55_01325"
     CDS             complement(4066..4920)
                     /gene="pstA"
                     /locus_tag="PLI55_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPA99511.1"
                     /translation="MKRNSRIQYFGFGFLYLAIFIAVLTLGAIIYFITVNGIKVINWE
                     FLTQVPRRAMTKGGIAPALVGTFYLTIGAIIFALPMGLACAIYLTEYSPKSVVINVIR
                     MSINNLAGVPSVVFGLFGLTVFVKFFGFGVSILSGSLTLGIMILPGIISAAQEALIAV
                     PQSLREASLALGATQWQTIRKIVLPSAMPGILTGVILSVGRAAGETAPIMFTAVTFYT
                     RGFPNSIMDEVMALPYHIYALMTEGTFPERQTMIAYGCALILLFLVLIVSGLAIYLRQ
                     KQRKYELV"
     gene            complement(4917..5801)
                     /gene="pstC"
                     /locus_tag="PLI55_01330"
     CDS             complement(4917..5801)
                     /gene="pstC"
                     /locus_tag="PLI55_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPA99512.1"
                     /translation="MKWNSEKVVKPVFGGLAFASLVFLLGIILILFQQGLPIFRIVNL
                     FEFIGGKAWYPTAEPAEFGILPLILGTLWITLGAMLFCIPVGIGSALFLHEIAGFRLR
                     SILKPIIEILAGIPSIVYGFFGMVIIAPFLQNLLDIPIGQCALTASLILGVMAVPTVC
                     SLAEDALNFVPKSFREASYALGANRWQTLTRVIIPAAGSGISTAIILGMSRVVGETMT
                     VLMVAGGAAVIPKSYLDPVRPMTATIAAEMGEAVVGSPHYHALFAIGLVLFLITMAFN
                     IIAEIISRNYRLKLGLGR"
     gene            complement(5883..>6397)
                     /locus_tag="PLI55_01335"
     CDS             complement(5883..>6397)
                     /locus_tag="PLI55_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPA99513.1"
                     /translation="IVAMDGIAVILHPSNKINNLTVEQIRKIYTGKISNWKEVGGDDK
                     KIVVLSRDTASGTFEAFSKLALNDEKVRQDALMNASNQAIATTVEKTPGAIGYVGIGY
                     ISSKVKAVTVENVTCTKKNILTKTYPLSRPLFMYTNGKPKGVVKDFIDFILSEEGQEL
                     VETAGYVGLK"
     assembly_gap    6398..6497
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6498..6850)
                     /locus_tag="PLI55_01340"
     CDS             complement(<6498..6850)
                     /locus_tag="PLI55_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPA99514.1"
                     /translation="MKKLFNLSILILAIASLLTAGEAVKIRMEGSTTVLPIAQLAAEE
                     FMIANPDVTISVQGGGSGVGIASLIDKTCDIATSSRAMKDEEIKNAVAKGVNPVAHIV
                     AMDGIAVILHPSNKI"
     gene            complement(6869..8107)
                     /locus_tag="PLI55_01345"
     CDS             complement(6869..8107)
                     /locus_tag="PLI55_01345"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HPA99515.1"
                     /translation="MKLRERKINIILALLGFCALTLNAGELDRLVNELVEKGVLDAGK
                     AQEILTLTEEEMRAEMNAGKSATLPKWIQNMGLKGDFRFRNQWEATEEDSVSRMRQRI
                     RFRLNGEAVVTSGFKVGFGLATGSSDPRSTNQTLDNSFETKPIMLDYAYGSYTFPKYA
                     NVLLGKFYSNLGFWTPTDYMWDSDITVEGVAAQFNHKMIFFNTGIYFLDEKGKVENVI
                     ADNPRMLILQPGFNMSKEGIYSLKAAATYYNFNKTKNMAFDHAKWSNSYITVNDSKYL
                     ALGYNNFNFATEVALYNKIFPYIGVCFEINQNLQAESDTAKNGIACGLTLGSEKIKDK
                     GQWQVKYVYRQLEKDAWLDFLPDSDTYGGKTDIKGSELIAQYGLNKNIVLALDYYKIE
                     NLHGAIKPETVLQTDIQFKF"
     assembly_gap    8293..8392
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8566..9108)
                     /locus_tag="PLI55_01350"
     CDS             complement(8566..9108)
                     /locus_tag="PLI55_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HPA99516.1"
                     /translation="MKVQLKKEIEQIVHLQDYAGKPTIEGVKVITLRRHNDDGGSFTE
                     LARITNGDFSGVENFKIAQINFSEMEPQSVKAFHVHRQQTDIWFVPPDSKILLILIDL
                     RENSATNGQRMRLVIGDNNSQLIVIPPGVAHGCKNLGLKKAHLIYFMNKQFSPEPSEC
                     DEWRLPWDYFGGEIWEVNKG"
     gene            9399..9770
                     /locus_tag="PLI55_01355"
     CDS             9399..9770
                     /locus_tag="PLI55_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99517.1"
                     /translation="MAITVFIISLFIGIFVLNLLILRYLKGSIHKSSLSVRLPKTVAA
                     FDRYTLKVVALVTLIIIIFMLIAIALQSGFVIPVIHPIALILFIFIAGVGSIYFFKSD
                     SNISKSDQKFSTGETDGEEQK"
     gene            9767..>10410
                     /locus_tag="PLI55_01360"
     CDS             9767..>10410
                     /locus_tag="PLI55_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99518.1"
                     /translation="MKSENVGNWQTLRFIFPDNAAISFDARIADSVIKELALDTGQPV
                     FKLRCSINELYQPNTQIFTSGFPYNDLWFRYAQILVIEKHLKLQAPVGIELIRVIFCE
                     FLRIAEYIFNLATLCLNGGLNDIFSGLMQQYESIQQYFEQILSGTNPMDFIIGHKKPK
                     DLTVGFTEMTLNLSHQLQIQLRKSLPHLLNNRLILSRFSKIADQTADNSAILND"
     assembly_gap    10411..10510
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10511..11516
                     /locus_tag="PLI55_01365"
     CDS             <10511..11516
                     /locus_tag="PLI55_01365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPA99519.1"
                     /translation="NANGYMNILFKNLIYGLFWVVIFVVIIPALSIIEQRYFTGHFRL
                     TEKKAKPIPSYLQYWGKFWSSLRGEKLLLSMTTQVFLWIETGYLMALWLVLPYQMQIQ
                     GNQTLWLFLAIITLPLNIINSIIASEEVKSEIGQAGIFKNGISLLSGFAPLLLSILIL
                     ITGNRYTSFWEISSQQSGYRLGFIPNWTIFSSPLFLLIGIIFLINLTMLMRLSDGSYK
                     PLEYEEPAEIYSGRHESFGILPQFNRSAMLFTLTTLAIFLFFGGWQTPFEPEGQNLAS
                     LTVGLIFFVKTILVFSACLAIKYHLPILTNWQIIKINLRIQIPVLTLIWLGLLIIRWL
                     "
     gene            11516..12004
                     /locus_tag="PLI55_01370"
     CDS             11516..12004
                     /locus_tag="PLI55_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99520.1"
                     /translation="MTNFQWMPVIFWLATFLTLLPAGLAITSTNPKNLILLNMFTQLG
                     IFLLIISLGVGFPAVIFLVLVFPLNIMMLYGHNLFPETNKQTARESRPAIILKTAVCF
                     LLFLLLIIAFYQNHLKNMLVIDNSGKILEGVIPDVSLLLILIGMFIMLILIAISHFLR
                     RK"
     gene            12001..12306
                     /locus_tag="PLI55_01375"
     CDS             12001..12306
                     /locus_tag="PLI55_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99521.1"
                     /translation="MSHLFLYQIVGIGLFIIGLYGLWRNRHCWAVITFLQLMSSGILL
                     SFLAITRTKGAYQSVSELIVIFIFAVLIIEILLVIVFTYKVLMSDSSSNSDDKSNRG"
     gene            12308..14197
                     /locus_tag="PLI55_01380"
     CDS             12308..14197
                     /locus_tag="PLI55_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99522.1"
                     /translation="MNILIILLLLLPICAYLLTRCLPDKCLLNRRGLSILFLLISGLI
                     GLGIFIVLLLINMPDYVQRISWTWANIAGHSLQISLLLNNFLGLLLALWALGSLIFAI
                     LTATDFFSPDHFRENITNLALGTLAVNGLILANDFLSLIFFWALIDLIIYRVVFRKIN
                     KASESYFPDIMVIGGHLLLILVTFSLFKISGTFRFDLIIEEVLSGKVDPQQLILPGSV
                     LILVGILRIYSPFFRYQFNSQTKMSISDEFILFCGLMPSGLFLVLKLFPIMPQIFLTG
                     LMIFSGISVLIFLIIHLAKTGFNQLLHLNTIFLISLLIITIGLGTFPISASLLFILFM
                     TNFMLIILAHDFQPMSDLANFSIALPFRLIAWLSLIGLPISLAFNTRLLLLQASLTLS
                     SNKPIHGTIISALLVLNYFLNVVVVFRIFLKSGWYEPKHRKIDQVAPLTHCIIAGISI
                     VCLYLFFTLPNFNPLTTELRFFPLFSIQGSQPLDYSQFSSNSLIIYSAILLIGLVVGF
                     ITAKYFKAEKFSRWLTEFSLDFRRYSMIPVANFSQVVARNIRHFDTVLFSRVSQSLMQ
                     TAQTAILFINRINAIGAQQITKLKEVPEKYLNRILDGIFNLKFQVLIGIVLSILIITV
                     LICIL"
     gene            14222..15700
                     /locus_tag="PLI55_01385"
     CDS             14222..15700
                     /locus_tag="PLI55_01385"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HPA99523.1"
                     /translation="MTEHLLSINLLIALIGLLGIALTKREKGNLIKTIAAIVTGLQFW
                     TIIEILFNFNPMDTALQFSERINWIPAFRIDYYLGVDGINLVFLIVTSLLIFLSVIIS
                     WNKREPLKEYFILLMILDIGLNGIFIAINLFLFLIFLGVTFFAIYLIIGFSNIRGSVN
                     SVNRFGIPFLISYIFILLGVLLLYKSNNLHTLNLTELTVGNAISHSKQMVISIILLIG
                     FAILIPLFPFHSWLIPLIKNGPKEVSLLILGIVTKVGLYGLVRIIAPVMPFATNSLTL
                     ALGIWGLVNIIYGAICALGSDDSNQILGYFTLQQLGFFLIGFATLGENSNISIAVTGL
                     SGSLLIVISHSCLATLLLIIFKNIRAENQNVARPSLPNIAIVTLTLLAGIGMPGFLDF
                     FARFLILLSAFQTQRLLILAIISLLGIFLNFAIYLKLFKKLVFSQQTRDSAKRLDSNE
                     VFASVSLLIVLILCGIFPSFLLNIIQPGITRLLEMFSTAIAL"
     gene            15708..17111
                     /locus_tag="PLI55_01390"
     CDS             15708..17111
                     /locus_tag="PLI55_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPA99524.1"
                     /translation="MSNFQSIIYFIPEIILIIAAIGFLLLTTFFKISDSKKYFLISVS
                     LIISLIATLLSHSADGHSIFNNLLVWDSYGQFIKIFTLIIACIILIIRRKENCNEASP
                     SPVTEFGIGLLLLISVAFVSINANHLVALFISLQILHLAGFFIVQSRNLSPRINTASW
                     HDLMFDIFTSLIMLMGIALIYDTTGTFFIAEIGQILSTVTRMNFPLILGIALLLVGFS
                     FTITGIYRLSWKLEILRTISASDRLLLLTIPQFIETTTLIRLLRVGLGLVGDSRPLTL
                     MIGIFAVLTLLFPIIWLIKLRSAKDIIIVTSIAQSGFGLLALCAGNISGDVAALFYEV
                     VLIFSLIAFTIGEILTINAKSSQLIALASLIGIPLTAGFPAKYLVFISLVQHSPFYYF
                     LIVLGIGSAALMLIGYVRIVRQIITAQGQFFNSPQKGSQLIFFIQILINIILISIGFY
                     WTPLTDYLQSIMTFCVP"
     gene            17200..18729
                     /locus_tag="PLI55_01395"
     CDS             17200..18729
                     /locus_tag="PLI55_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPA99525.1"
                     /translation="MSPQNKRFFFWFVIAFLIIFIILISISLIHNFNKSKIISLPAQE
                     MRGVWMSRFDYTEPFPSYNPDSMKTYISDSFRRLKDANFNVIFFQVRGNADAFYASRY
                     EPWSNLLTGKLGQNPGWDPLAYAIETAHQLGLELHAWLNVFPAWRGTAPPTTSQPLHP
                     YLAHPDWLICDGNGKKMLLSDGYVSFSPGIPAVHNHLIKVISDIVSRYDIDGIHFDYL
                     RYPEQSELKGYSHDPISWARFQSHEGNPLGLDWTDWQREQINEFVARAYNCVTDIKPE
                     IKVSAAVIGSYKTDHWNGYSAGFQDPRRWLEIGKIDLIIPMTYYHLNHKNFTFNKALK
                     EWQSVIDNPDQIFPALAAYNLSWEEIIEEIHLVRNSHFKGMVFFAASSLDSEKLQSLQ
                     TTEFQYPSIFPCLTWKDSVAPPSPINFAVEKLTSDSLEFIWQIPHQSNDIKKFVIYQS
                     KNLPIDLTKGENILAIVAGSDTSYQTKFNLPDQRRYYYTITSLDAAYNQSEPASVIKI
                     K"
     gene            18946..19896
                     /locus_tag="PLI55_01400"
     CDS             18946..19896
                     /locus_tag="PLI55_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPA99526.1"
                     /translation="MKVKFWGVRGSIPTPPTSEQIRHKFLRIIDNLPTNIRKNPTEIK
                     KYILSLSYLEAGLIGGNTSCVEIRADNKILIFDMGSGLRALGNYLVKNEQNKNGLDLH
                     IFISHTHWDHIMGFPYFTPAFFPNNKITFYSPHPDIKERLEIQQDSRFFPVSLEHMLA
                     KKEFIQLQPKTSITLGKVKISNYPLYHPGGSYGYRIDWDGKSVVYATDSEYKNLDRES
                     TKHYIDFFRGANLLIFDAQYTFEEAIHKEDWGHSSALIAVEFAVEAGVKKLALFHHEP
                     ERDDFEINDILKKSRDFKKINFPNEILDIFLAMEELEITL"
     gene            complement(20168..22129)
                     /locus_tag="PLI55_01405"
     CDS             complement(20168..22129)
                     /locus_tag="PLI55_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPA99527.1"
                     /translation="MRRNFFHNKTKLLFQAIILLLIIILVIIGCTDKAKKADFEAYCP
                     FGGLVSLGSKFHLGSMSCSMNETQVFMGIFLLIGVILIGKLFCGYICPIGTVTEWLNK
                     LFARFKFSIVLKGKADRILRLGKYVLLFFTAYFTATASELWCKKFDPYYAAVTGFGSD
                     TVLLVGILTIFTVVVLSVFIRFFWCKYICPLGALSNIFSNVLITVPILMIYFGLYIFG
                     IKLHILWLLLALCLSGALTEILRFKFFSISPFRININKNTCTTCGNCDDNCPQGIPVS
                     SYEKVTHPDCTLCLDCTKECSSSGSIRLNKGNYGWLPPVALVVLFILALLTARNFEFK
                     TLSERWGNYAELESVQKFEMEGLKSVKCWGSSKSLQNKLMKQPGIVGLDTYVKSHKVV
                     VYYDSTKIDEYGIKKAIFTPYQYKIQKFDEYQPEKIAVFEIPISGLWDVQDNADLIRM
                     LRVNPKIVGFETNFGEPVIARIYVELGKVTPDSIIYLINQKSYEKKLPNGKTEVVKVN
                     FRCEGRGAFVDTLDYVTFRRRLFAGYDQAFNSYEKYRPADLMIFEIGLPDAESIAVRR
                     ALKYLTSHISFFDGVVRVRTDYTDREALLIYFDPTQVNPKDIQTKLNEPMLKILTSDE
                     KIMEMENSFEFREPLRIYQVTPEMYALEK"
     gene            complement(22246..>23126)
                     /locus_tag="PLI55_01410"
     CDS             complement(22246..>23126)
                     /locus_tag="PLI55_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPA99528.1"
                     /translation="NNFIAMNTTNNGSATDNRVYGIAFGSANWSGEANIYHNTIDIAP
                     TNQTGVHAAIGTEIPFSAKLHIYNNILSNRHDAANSFGIHWHNTVSPNSGLASNYNNI
                     YIAGSSASVGFYGSTPCADLTAWRAASGQDANSINKPVNFVSATDLHVTGSSIGDNDL
                     ACHRPDWLSTDIDGDARPNVTYMGADEGATALSFVGVANGSSGLPTEFSLRQNYPNPF
                     NPTTVIAFALPKSTQVTLMVYNMLGQPVAVLQNGFLKAGYYHVNFDASNLPSGTYIYR
                     IQAGDYRNVKKMTIVK"
     assembly_gap    23127..23226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<23227..25967)
                     /locus_tag="PLI55_01415"
     CDS             complement(<23227..25967)
                     /locus_tag="PLI55_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99529.1"
                     /translation="MSVNKASFIDANTGWIVYSTTYPSKQMGKVQKTTDGGAKWTTVR
                     EPDVSANSWNDMEFIDANVGYACGGGGVIYKTTNGGSDWTMIGDTNTYKFDLYSLDVV
                     NANVVYCAGKSGTVLKTIDGSTFTKLSYNFDGDMTASDETRDDLDGGIAFFDENRGVV
                     AVDFNKEATIWWTEDGGTNWHPVKIAAAFPPGVTSYRVYDVAVGGDSTVALACYHRVC
                     LVSTNGGRTFANKGVVQFANVYTSYVEVFDNNTIIYGGGSDNFGAITTNGGTDWTNFS
                     TGTGQRLLSADFIDARTGYWFCEYQQWMKTADGGVNWIPINNWPGISWLGLAFPDEKK
                     IVLTADGGGEMTFSNDWGKTWSYPNNLRTGLDGDIFECEFIDANNGLIGGSGGYILKT
                     TDGGATFTQIDNPMYNNNKAVNAMHYLNSDTVFVGGGSGYICYSFDGGDTWTKATAGS
                     KTVYDIWPLSNNAVVATCASGEYYTSTDLMTFTKLGATSEAKNLRAVEFRNGIGIIPG
                     ASGYIYRSTAWDAMPTLVYTASNGAELYDVEFVDDNTVFVVGQYGTILKSEDAGLNWT
                     VETSPTDEVLQKVRYSDNGSVGILWAVGQNGTILKNVIYSNVLAGDYYIPQGDHEQGF
                     ATLGAAVTALNTYGAIAPVTFYIDADLIEPGSELIIDREDLTAETALTIKPASGKTPS
                     ITINDFPTTGNYSGQGFTIENTAYVTIDGSNTSGGTSRDLTIIGNDTDGKCVIGVIDN
                     SDFITVKNVNITFENLLASGSILGVDGYTGVPNSLLIKNCQIGTPEKTVANGVALRGN
                     SPETPCGAVVMGCDIYAAGTGIMADYISKNAYINNVISIINPLADQTFYSGMYLNGFA
                     AGDTCVVFNNRIVNVEMNTTAPQFAGGIVIGENGGVIDIFNNFIAMNTTNNGSATDNR
                     "
     assembly_gap    26027..26126
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26127..27430)
                     /locus_tag="PLI55_01420"
     CDS             complement(<26127..27430)
                     /locus_tag="PLI55_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99530.1"
                     /translation="MIGLVFITPLFAIDNPLGREIQPRNLMNQYNLTIYDLSGNPAFY
                     GISYPDNLDFYQMQATNDNNVFRRLYDPRRQEDYALSFHSAKHLSERNTLVTGIRFNR
                     TIQRDLYHSLEKNIYDEYIAFLDTTTGTTTYNSPQINFLYDYALTKRLHLGLEINYGV
                     EQSLKDVYTQCEIIARNTELRGGLSYISKNQKIIGGLLGTYYNAQRKYEAVKYYQDAF
                     VRTLFGYHIYKDETPKSINRKNDEREGGGLALQFCHRDFMLPGLALIITGEYNGRSDN
                     VTAGSPQKPTPRGHWVREIRRGILDLNYSPIESNNSLEFIYEIRSTDDWVKSGNYEVI
                     ILDNAELLNVFSLRWQTRLFKRLKMNIGASYASQACDYQEYTIPFQYHETDYHSSAFL
                     DLNYAINQILSVYFLGNLAIDDLYFYWNAERATRYGCEIGIER"
     gene            complement(27475..28593)
                     /locus_tag="PLI55_01425"
     CDS             complement(27475..28593)
                     /locus_tag="PLI55_01425"
                     /inference="COORDINATES: protein motif:HMM:NF027540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4876 domain-containing protein"
                     /protein_id="HPA99531.1"
                     /translation="MVKKAALLTIILLLNLTNCFHQKPTEFDSTYTLKLVLVYRDSTL
                     GYDERLSKVDVKIHTGDYNLDDYCATTNDSGVVVFENLPWAIFDPQVKAKVELPFIDS
                     FGNRRLDSLISYTLIDTIVAITPSETGIGTDTIYTITANLRPGLKINEIYYTGPENRN
                     NWFYDQFIELYNSSDEVQYLDGMIVCRIYHMLTSVTYAFRFPGTPLTGREYPVQPGEF
                     VVLAQDAVNWLELYSTSVDLSGADWEFVNAMDFGDIDNLNVPNLANIVPGRTVDFMIN
                     LSTNVIALADGSDSVMTDGLDIGTVVDCVEYNSKSPSEQTTKNIDKILDAGWAGVGNQ
                     KYSGQSVERILPGFDTNNSFNDFVIIKPPTPGYHHPSP"
     gene            complement(28587..31193)
                     /locus_tag="PLI55_01430"
     CDS             complement(28587..31193)
                     /locus_tag="PLI55_01430"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA99532.1"
                     /translation="MKKQVIIILILTGLAIRGIASTITGKVTCQMERKPIPDALVILQ
                     KRSDPDFSRTTVSDKTGQFTLTNVEPGVYNIEAVREGYFKNVLFDLKIEADRNYEINI
                     KLLKQAGKSSSDYCFMLGGIEVYSVQKELIPESPVTTRTINTGEIEHMQATSLGDILS
                     LVPGVDKSQNPGLSGRSEIGLRSVTAAGVSMSAVESFGSTIIINDNQISTDANTSGNV
                     YYGSATRGLDLRIIPADNIKSVEVITGIPSVEYGNFASGVIKVETKIGRIAPKFNAKI
                     NPDTKNASFSHGIKLGRSILDYHLNYGYSERDLRKVGDEFQRLHASGNLSGAYLDQKL
                     ETHIGGSFTKLLDNEKPTDVYQMASYDKGFLATGDFNFIYKDSEAKSWKGVFSVNLNN
                     KRNYKSKWVAENYILKYDTTVVDSVTGIAIDTTLIKILPGFIGKMKEIGEEWKIAGKI
                     QRKIALTTKHTKHNLLFGGEVNYEKNTGDGVVIDENFPYYGQYSSHRSYAFDDYPDLT
                     SLSFYAEDVLEGKMLARRYKLMAGLRYDAYSPIGFNFKNMFKEKAFLKSEHGDFLSPR
                     LNLQYSITEDWQMRLGAGRSVKDISLGHIFKAPDYFKYASGDTIIEEVHLQYNPDLQA
                     YSIDKYEASLDWKFSDLIGFSLTGYYQASDNMPVTVSYPYGYEINPDTITAAAWERFE
                     NRGWSRSNGIEFTMRTQRIHNFQYRLNVTYRFNKNGKTGLVYDANPENWEDIWYKPES
                     YWSEKIIIDYQINYISNRLGIWVTLDAQQVPLEHFKRIYRSNSTYKTENGVTYLWYQG
                     MTYYYERYLRDTGGRWLFNFRVTKSLSQSMEISLFVNNILDDRAVWKNFDGSLNEHNP
                     PIFYGLEVSAQW"
     gene            complement(31208..33988)
                     /locus_tag="PLI55_01435"
     CDS             complement(31208..33988)
                     /locus_tag="PLI55_01435"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPA99533.1"
                     /translation="MKWFRSLAYTIAIILIIFSSLLALDESRVKEFTLNNGLKVITYE
                     MHTAPVIYSQLTYNVGSKYEPFGKTGISHVVEHMMFKGTNRFPKGTISELISANGGIF
                     NAYTSTDITVYYELLPQNKIDLAFDIESERMYKCAFDPQEFESELKVIMEERKQRTEN
                     SAVGLRREEINTLIYKNHPYRNPIIGWMEDIRSINRDDAYKYYRTYYTPNNATLVLTG
                     DFNTDEIMQKVEAYFGKIPRGPEIPAYNFYRVPICGKRTLDFIHPDILSESIHYYFEA
                     PTRFEADGPALYVAGQILGARSSTSRLNKKLKREKQVCESAVAGLSFTKDPSTFNLIT
                     SLMPTAQISEVENLIWKEIDSLATTAVLDYDLQKIKNQIVFNELTGNQHNEKIGGQLA
                     TYDNFYNWRYINQWNQLVMAVTADDIIRVVNKYLKTGNYVACYSHPDTTQKKVGFLAA
                     ESDESEFDLEITEPETIEESEDTTGKYVNPEVRQIYFPKLDEVISPHPIAPLVTTAKL
                     KNGVPVYFLENHEYPTILLIGYIETGRLEEENLHPGIRQMTVGMLSRGTAKRTYEELI
                     EERSFIPYQIDISQSWNKIIFQGYGLSKDVDKILQANFEIVTQPTFPESEIEKVRPSL
                     IKTAKEYKETDRMKAFYAMFEKVFEGHQYALPYAGDPETYRILTSADLKKFHAKYYAP
                     DNLKLLVVGDFNRKEMLSRLNATYGSWKHKSGDKHLPFQTVKPIQGKTVYVFSNPGYK
                     QCRVDIAFCPVEGGIKTDNPDIEALKLLESILCGSSLTSRMGKELRDKQGLSYSIKSN
                     LWIRDHGGYWNIRTELDQANLSKMIHGIFAEIEKVQREGVTDEELEKAQARRIAMLTM
                     NTRTPDEIGAVIFDLIQEKKPLDYFDHSKERILAVTKADIQRVANKYLDTQNYIIAVS
                     GNMAPDALNEFK"
     gene            34536..34606
                     /locus_tag="PLI55_01440"
     tRNA            34536..34606
                     /locus_tag="PLI55_01440"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34568..34570,aa:Gln,seq:ttg)
     gene            34631..>35526
                     /locus_tag="PLI55_01445"
     CDS             34631..>35526
                     /locus_tag="PLI55_01445"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPA99534.1"
                     /translation="MKNNGIIFCGRSNPQLGYRINEILGIKPGAIKISNFSDGEITVK
                     LEDNIRGKDVFIIQSTNPPAENILELLLIIDAARRASARRITAVLPYYGYARQDRKDQ
                     PRVPISARLILDTIVAAGANRIMAMDLHSTQIQGFVNIPFDHLYAKPIFVEPLRKLIG
                     KRDFSIVTPDVGGIKVARSYAKILGLPITIIDKRRPKPNMSEVMNVIGEKDMKHALII
                     DDMIDTGGSITEAAALLKEYGTETITAVATHGLFSGDCVKRLSDSPIDRVIVTDSVAI
                     PEEKHFAKLQILSAAPMLAEAI"
     assembly_gap    35527..35626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <35627..36112
                     /locus_tag="PLI55_01450"
     CDS             <35627..36112
                     /locus_tag="PLI55_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPA99535.1"
                     /translation="DLNINKKSHKCIIRDVQFDPLTEEIIHADFMGVSLEEMVTVSIP
                     INVTGTAIGVKTFGGILEQHLWELEVKCKVSQIPDRVTIDVTNLNLGDSINAGSLNIE
                     GVEILIPATTSIVSVVKPTGTKAEEEVAKEEVTEEVGAEETAGTQEAKETKETKGKTE
                     K"
     gene            36138..36779
                     /gene="pth"
                     /locus_tag="PLI55_01455"
     CDS             36138..36779
                     /gene="pth"
                     /locus_tag="PLI55_01455"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPA99536.1"
                     /translation="MISAIIGLGNPGKKYQHNRHNFGQMCLNHIARRHNLAFKPGGGE
                     RAFTYATTVIADRTVYLCQNTTFMNNSGLAVRQILNQFDLQTDEILVVYDDIDLPLGK
                     IRLREKGSAGGHRGIRSIIETLATNQFARLRLGIGPQDEGVPSEDFVLTDFRPAEKEL
                     VEAVLEESYKCILTILNNGIRPAMEYFNRRALHLAPKKNEIQVNSIKKPIILT"
BASE COUNT        10451 a         7716 c         7203 g        10840 t
ORIGIN      
        1 aattaaaata attcacaata actcataatc aaatttttca tcgagaaatt ttacttaaaa
       61 tatgccttaa agtcaatcac ggatttgggg aatgagcgga atttaccaca aaggcacgaa
      121 gacacgaaga cattaatgca aaaatccgaa ggctttgagt ggtcttcttt atcccctaca
      181 gggaatagct ctgcgatttc cattgatcta caatacctta accgctatgt ggtatccatc
      241 actattgaat gactacgaat cgctatcgaa caaaatactt ctatgatttt ttcaccgcag
      301 agcggtattt ggtaatctaa tgaccatact tatttgacct ggatgggtta aaccttcatt
      361 aaattgggat aagtcacgat ttaaaatatc ttaccctcaa aggatttttt aaaataggaa
      421 aattaaatta agttatcgtt ggattttaga agaggttatc aaatgaaaaa gaaaagtaaa
      481 aaatacaacc ctttacggat ggaaaaggaa ttgtctgatc taattgagta tattgagaat
      541 gctgggtttg aatcgaagga agagatagac cgctttttag cgaaaatggg aggtaaaaaa
      601 attggttcag ttctaaataa aaagaaaaca gccgcagacg aagcatttga attgattctc
      661 gaagcccaag aagtatcgcc tgaggaagga attaagctgg caaaaaaggc tataactttg
      721 gatccgaatt gtgccgacgc ttatactttt atagccgatc atactgagga tgaagatgaa
      781 gccctagaat tatatcaaaa agcgatgcaa gttgcgcgtg aatccttagg agagaacgcc
      841 tttattgaat atgtcgggca tttttgggga attaccaaaa cccgtccata tatgcgggct
      901 aaagccggtt atgcgtcttc cctaatttta aaaggtgatc ttcaatcagc tatcaaggaa
      961 ttccaggaaa tgttagtctt gaatccctcc gacaatcagg ggattcgatt ttcactatca
     1021 gtgctgttgc tccaattgaa ccggcttcgt gagtatgaag atttattgaa acaatataaa
     1081 gatgaaaata gcgctggctg gctatataac cgcgtcctct atttatattt aaaatatgga
     1141 ggcactacca gaacgatctc agctctggaa gaggctatcg ccgagaataa atatgtaatg
     1201 gatttgatca ccggtctcga agaaccgccg aaagaattac cggaaagcta tggcatcggt
     1261 agttacgaag aagccgtctt atattactgg gaatctattt taacttggat ggcgcataaa
     1321 aaagccattt tatggcttat ccgttactgg gagcgacgcc gggaaatcaa ttgaattaag
     1381 tcctatcgaa tttctctaaa aatagcagaa taacgttaaa aaacattcag gaatattttt
     1441 cggatttggt gagtcgctga aggtttaaca ataaatcaaa aagtcaaaat cagcctttaa
     1501 atacttggtt gcgaaaccag attgatattc cccagaaaag ggcaattcca ccagcaagaa
     1561 ggacaaccaa gtctagaaca catagtattc ggagacagct tatattgaaa attttgggct
     1621 caccattgac ttgctgtacc aataagtaaa ggcaaatcag agaaaccgaa aggacaatta
     1681 accctccccc aactaaaacc tgcgaccggt gccgcaaccg ccttggtaat atttttgcca
     1741 tacactccct cgctaaaaac cttacctacc gttaattaaa actaatactt tatatcttaa
     1801 ggagaatctg gagggaaaac aatgaaaaat ttaatggatt aagataatcc taaaacaatt
     1861 agccggcttt aatccttagc cagagtaaaa gcccattcat cagcatgaaa aacccgacta
     1921 tacaccaaag aatcgccgcc agcgctacac ttcgtacaat ccaaagaccg aggtaggtaa
     1981 ttaatgcttg gtgtgccagc gaaataataa gtacgaaaac gattggtagg aaaaaattaa
     2041 ttaaaccact tctcagagat cttttcagct tttcccggtt atcgcggtgg tcggagtggt
     2101 gttttcttcg gtgtcgttta gtgtgctcca tactatctct tgttattgcc cattataaaa
     2161 taaaaaaaat gttctatttg gtcaagctaa agttttttat cctgcttaat ttataaatcc
     2221 ataaaaagct gttgataaaa tccagttttg ttattttata gatcggaata tgaaaatatc
     2281 ggtgaaattg cgaagtgagt tgggaaatat gatcaagaag cggtgttaaa aactttgagc
     2341 atccagagaa aatagaagat gacacaaatc aattggtttg tttcagttat taaataacac
     2401 atcataactt aatacattac agtgtattaa ataaatatct ttattcttat gaattattca
     2461 gggtagaaag taaatctttc attgccaagt tttctcttta tgccaatact acaatcagca
     2521 ttaaaatcat tttcgaatta tattaggaaa ccttcagaat gtttatttat ggtccttatc
     2581 tatttataga tctccgcctc aactttctaa aggtggaaag ggctacaact ttactcctca
     2641 attttttccg tgcttgatta ctctgccctg tactataaaa attatatcct cacagatatt
     2701 ggtcgaaagg tcggcggcgc gttccagttt gcggctgatg tccatcaccc gtaaggcaat
     2761 cggcacagtt gccggttgtt tgctcattaa ttgagtcatc tcggcaatta ttttatcccg
     2821 cagatcgtca accacatcat ctcgtttgca gatttcttgg gctgattcgg catcattatt
     2881 gataaaggca tcaatgctat catgaagcat cccgagcgat tcctgcgcca ggtgatttag
     2941 aagcgggatc ggcttaaagc tcggtctatc aatcaggtaa agagcatcac gacaaatatg
     3001 caccgcccca tcaccaagcc gttcaaggtc attactcatt tttaaaatca taataattgt
     3061 gcgcagatca cgcgctcgcg gctgatactg agcaatggtc tgaatacaag cttcatccat
     3121 tcgcaaatcg aactcatttg cttttgattc atcaacatta atgatttctt tcagcgtatt
     3181 aacgtcctta gcactcagcg cttgcagact tcttgtgatc atactctcga ctaatgccgc
     3241 atattcgact atggcttgtt ttaattcttg aattttcttc gttaacatat tttacctcat
     3301 catattttct attaaccaaa cttgccggaa atgtaatctt cggtgtgttt attttccggt
     3361 atggtaaaaa tcttctcagt aggcccaaat tcgatcaatt cgcccaaata cataaacgcc
     3421 gtataatcgg agacgcgcgc cgcctgttgc atattatgcg tgacaattac aatggtgtaa
     3481 cgctgtttca actgttcgat caattcctcg atacgcttag tcgaaatcgg atcgagactg
     3541 gcgcagggtt catcgaacag aataacctca ggctccactg ccagacagcg ggcaatgcac
     3601 agacgctgtt gctgaccgcc agaaagccgc aaagcgcttt cattcaggcg atctttgact
     3661 tcgtcccaga gtgcggcttc ctctagtgaa cggcgaatgg tattttcaat tgagtcggta
     3721 cttttaatgc cattaatctt caatccgtag ctgaggttgt cgcgaatgct tttcgggaag
     3781 ggatttggtt tttgaaaaac cataccgact ttacggcgca aagttacaac atccacaccc
     3841 gggtcgtaaa tattatgatt atcgagtaga atccgacctt ctacccgggt attgggaatc
     3901 agatcattca tccgattcag cagacggatg aacgttgatt tgccgcaacc ggatggccca
     3961 ataatcgccg ttacccgatt ttggaaaatc gacatattga catttttcaa agtctgttta
     4021 tcaccgtagt aaaaattaac attttccgcc tgaatacgga tttccttaga caagttcata
     4081 tttcctctgt ttctgccgta aataaatcgc cagccccgaa actattaaca ctagaaataa
     4141 aagtatcaaa gcacaaccat aggcaatcat cgtctggcgc tctggaaaag taccttcggt
     4201 catcagggca taaatatgat aaggcaaagc cataacttca tccataattg aattgggaaa
     4261 accacgggtg taaaaggtta ccgcagtgaa cataatcggc gccgtctcgc ccgccgctcg
     4321 accgacgctt aggataactc cggttaatat tcccggcatt gccgacggta gcacaatttt
     4381 gcgaattgtc tgccactgcg tcgcgcctaa tgccagtgat gcctctctaa gagattgcgg
     4441 cacggcgatt agcgcttcct gagcggcgga aataattccc ggtaatatca taatgcccag
     4501 cgtcaagcta ccggataaaa ttgaaactcc aaatccgaaa aactttacga agaccgtcaa
     4561 tccgaaaagt ccaaaaacta ccgacggcac ccccgctaag ttattgatgc tcatccgaat
     4621 aacgttgatc acaacgcttt tcggactgta ttctgtcaaa taaatggcgc atgccaaacc
     4681 catcggcaaa gcaaatatta tcgcgccgat tgtgagataa aaggtgccga ctaaagccgg
     4741 tgcaatgcca cccttggtca tcgcccgtcg tggcacttgc gtgagaaatt cccaattaat
     4801 gaccttaata ccattgaccg taataaaata aatgatcgcg ccgagggtaa gtactgcgat
     4861 aaaaatcgct aaatataaaa atccaaaacc gaaatactgt atgcgtgaat ttcttttcat
     4921 cgtcccagcc ccagtttcaa gcgataatta cggctaataa tttccgcaat gatattgaaa
     4981 gccattgtaa tcagaaataa tactaagccg atggcgaata gtgcgtgata atggggactg
     5041 ccgactacgg cttcgcccat ctcagccgca attgtagccg tcatcggtcg caccggatcg
     5101 agataacttt tcggaattac cgccgctccg cccgcaacca ttaaaaccgt catcgtttcg
     5161 ccgactactc gactcatccc gagtataatt gccgttgaaa ttcctgaacc ggcggcagga
     5221 attatcacgc gcgtcagagt ttgccagcga ttagcaccca acgcataaga ggcttcccgg
     5281 aaacttttcg gcacaaaatt caaggcgtct tccgccaaac tacaaaccgt cggcaccgcc
     5341 attactccga gaatcaagct ggcagtcaag gcgcattgtc caatcggaat atcgagtagg
     5401 ttttgcagaa agggcgcaat gattaccatc ccgaaaaagc catagacaat cgaaggaatc
     5461 ccagccaaaa tctcgataat cggcttcagg atcgaacgca ggcgaaatcc ggcaatctca
     5521 tgcagaaaaa gggcactgcc gattcccact ggaatgcaaa atagcatcgc tcccagcgta
     5581 atccataaag tacctaaaat taatggcaga atgccaaatt cagccggttc cgccgtcgga
     5641 taccacgcct taccaccaat aaattcaaaa aggttgacaa tacgaaaaat cggcaaaccc
     5701 tgttgaaata gaatcaaaat aattcccagt aaaaacacca acgaagcaaa tgccaatcca
     5761 ccaaatactg gttttactac cttttcgcta ttccatttca tatttagttc tcataatgac
     5821 ggcatggcag acatttgcct gccataccat aaattaatac tatacaaaaa aacttcccga
     5881 tactatttta atccaacata tccggctgtt tcgactaact cttgcccttc ctcactgagg
     5941 ataaagtcaa taaaatcctt tacgacgccc ttgggcttgc cattggtgta cataaagagt
     6001 ggccgcgaaa gcgggtaagt cttagtcaaa atgtttttct tggtgcaggt tacattctca
     6061 acggtaacag ccttgacttt gcttgatata taaccgatac cgacgtagcc aatcgcaccc
     6121 ggcgtctttt caaccgtggt tgcaatggct tgattagagg cattcatcag cgcatcttga
     6181 cggacttttt cgtcattaag agctagttta ctgaaagctt cgaaagtgcc gctggcggta
     6241 tcacgggaca gcactacgat tttcttatca tccccgccaa cttctttcca gttcgagatc
     6301 ttaccggtgt aaattttccg aatttgctcg acggtcagat tgttaatttt attcgaggga
     6361 tgcaaaataa ctgcaatgcc atccatagct acgatatnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6481 nnnnnnnnnn nnnnnnntta attttattcg agggatgcaa aataactgca atgccatcca
     6541 tagctacgat atgtgccact ggattgacgc ctttggcaac ggcattttta atctcctcat
     6601 ctttcatagc gcgcgaagaa gtggcgatgt cacaggtttt atcaattagc gaggcaatgc
     6661 caactccgga gccgcctccc tgcactgaga tcgtcacgtc cggattagca atcataaact
     6721 cctcagcggc taactgcgcg atcggcaaaa ccgtggtact accttccata cgtattttga
     6781 cagcttcacc agccgttaac agcgacgcaa ttgccagaat caaaattgat aggttaaata
     6841 atttcttcat ttttagttcc tttctttgtt agaatttaaa ttgaatgtca gtttggagaa
     6901 cggtttcagg tttaatcgct ccgtgtaggt tctctatttt atagtaatcg agcgccagga
     6961 cgatattttt attcaaaccg tactgggcta tcagttcgct ccccttaatg tcggtcttac
     7021 caccataagt atcggaatcc ggtagaaaat ctaaccaagc gtccttttca agctggcgat
     7081 aaacatattt cacttgccac tggcctttgt ctttgatctt ttcggaaccc aaagttaaac
     7141 cacaagctat gccattttta gcagtgtccg attccgcctg gaggttttga ttaatttcaa
     7201 aacagacacc gatataggga aagattttgt tgtatagggc aacttcggta gcaaaattga
     7261 agttattgta gcccaacgcc agatatttag aatcattgac ggtaatataa gaattagacc
     7321 atttggcatg atcgaaagcc atattctttg ttttattgaa attataatag gtcgcggcgg
     7381 cttttaaact gtatatacct tctttactca tattgaaacc gggttgtaaa atcagcatac
     7441 gcggattatc ggcaatgaca ttttctacct tgcccttttc atccagaaag taaattccag
     7501 tattaaagaa tatcatttta tgattgaatt gcgccgcaac gccttcgacg gtaatgtcgc
     7561 tatcccacat ataatcggtc ggcgtccaga agccgagatt gctgtaaaat ttgccgagca
     7621 gaacgttcgc atatttggga aaggtgtaac tgccataagc gtagtccagc ataatcggct
     7681 tggtttcgaa ggaattgtcc agcgtctgat tggtagaacg cggatcactg gaaccagttg
     7741 ccaatccgaa gccaactttg aatcccgacg tcactacggc ttcaccattc aagcggaagc
     7801 gaatccgctg gcgcatccgc gaaacggaat cttcttcggt ggcttcccac tggttgcgga
     7861 atcggaaatc gccttttaat cccatattct gaatccattt cggtaaagtg gcagattttc
     7921 cggcattcat ttccgctcgc atttcttctt cggtcaaagt caaaatctcc tgagctttac
     7981 cggcatccaa cacgcccttc tcgactaatt cattaactaa tcggtcaagc tcaccggcgt
     8041 tcagcgttaa ggcacaaaag ccgagcaacg ctaaaataat gttgattttc ctttctctca
     8101 acttcatttt tactcctttt tgttggttga ttcatttttc acgccgataa ttaaggaatt
     8161 cattttaaga cttggtaaac gaacagttaa tttttggtta agaattatta attacaactc
     8221 tggcacagtg aaaagaaagg aaaaaggctg agaatggcca ttagtcattg gtcattggtc
     8281 attagtaaag aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtcattgg
     8401 tcattagtaa agaaagttga ggcagagaaa agaattttaa tcttagcttt tcttttaggt
     8461 taagactgat aaaagtcatt acttagccaa aagaaagctc ccacgaggcg gactatttgt
     8521 aatcaaagag aaagggaaac tgattttcgc ttctgaattg attttttagc ctttattgac
     8581 ttcccagatt tcgccaccga agtaatccca gggcagacgc cattcgtcac actcggacgg
     8641 ttcgggagaa aattgcttat tcataaaata gattaggtgt gcttttttga gtcccagatt
     8701 tttacagccg tgcgccacgc ctggtggaat aacaattaat tgtgaattgt tatcgccgat
     8761 gaccaaacgc attctttgtc cgttagttgc cgaattctcc cgcaaatcaa ttaaaatgag
     8821 cagaatctta ctgtccggcg gaacaaacca gatgtcggtt tgctgacggt ggacgtgaaa
     8881 agctttcaca ctctgcggtt ccatctcgct gaaattgatc tgcgcaattt taaaattctc
     8941 gactccgcta aaatcaccat tggttatgcg cgccagttcg gtaaacgaac cgccatcgtc
     9001 attatggcgc cgcagagtaa tcaccttaac tccctcgatg gttggctttc cagcataatc
     9061 ctgcaagtgc acgatttgtt caatttcttt ttttaactgt actttcaatt tatgcaaccc
     9121 cttttcttca aaaattagat aactggtaga aattatcaca ttgttactta aaaaaccaga
     9181 taatccgctt caggcgcata ggaatcaaga ctttgctcaa tatgcttatc tgctatctaa
     9241 ttgataatcc gaatctgtat ccctacaggg aataaatttc cgacaaatct tttaccttgg
     9301 agcgattaag ctttctaata aattagtgaa agtcttgtat aggattgctt gacattcgcc
     9361 tattctgctg gtaaattttc gacagttttt ctaaatatat ggcgatcaca gttttcataa
     9421 tatctctatt tatcggcata ttcgtactca atttgctgat tttaaggtac ttaaaaggat
     9481 caatacataa atcatctctt tcagttcgtc tgcctaaaac ggtagcggct ttcgatcgct
     9541 atactttgaa agtcgtggcg ctcgttacct tgataattat catttttatg ttaattgcca
     9601 tagcactcca atctggattt gttataccgg tcatccatcc tatcgcgcta attttattca
     9661 ttttcatagc gggggttggc tctatatatt ttttcaaaag tgacagcaat atctcaaaat
     9721 ccgaccagaa attttctacc ggagagaccg acggtgagga gcaaaagtga aaagcgaaaa
     9781 tgtcgggaat tggcaaacct tacgttttat atttccggat aacgccgcca tatcctttga
     9841 cgcccgcatt gcggattcag taatcaaaga actagcgctg gatactggtc aaccggtttt
     9901 caaattgaga tgttcaataa atgaacttta tcagccgaat acccaaattt ttaccagcgg
     9961 tttcccttat aatgacttgt ggtttcgcta tgctcaaata ttagtcatcg aaaagcatct
    10021 taaacttcag gcgccggttg gtattgaatt aatccgcgta attttctgcg agtttcttag
    10081 aattgccgaa tatattttta atttagctac actttgctta aatgggggac taaacgacat
    10141 tttttccggt cttatgcaac agtacgaatc cattcagcaa tatttcgagc aaatattaag
    10201 tggaactaat ccgatggatt ttattattgg acataaaaaa ccaaaagacc tgacggtcgg
    10261 atttaccgaa atgactttga atctctcgca ccagctccag attcaactcc ggaaatcttt
    10321 gcctcattta ctcaacaacc gcctgattct ttcccgattt agcaaaatcg ccgaccagac
    10381 cgctgataat tcagccattt taaatgataa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn aaatgccaat ggttatatga atattctatt taaaaatttg atatatgggt
    10561 tattctgggt agtgattttc gtagtcataa tcccggcatt atcaattatt gaacaacggt
    10621 attttaccgg ccattttcga ttaacggaaa aaaaagcgaa accgattcca agttatttgc
    10681 aatattgggg caaattttgg tcaagtctcc gaggtgaaaa attacttttg tctatgacta
    10741 ctcaagtttt tttatggatc gaaaccggtt atttaatggc tctttggtta gttttgccct
    10801 atcaaatgca aattcagggt aatcagacgt tatggttatt cctggcaatt atcaccttac
    10861 cgctgaatat tatcaattca attatcgcta gtgaagaggt aaaatccgaa atcggccagg
    10921 ctggaatttt caaaaacgga attagccttt taagcggttt cgccccgttg cttctgtcaa
    10981 ttttaattct tatcacgggt aatcgttaca ccagtttttg ggaaatatca agccaacaga
    11041 gtgggtaccg cttgggtttt attccaaatt ggactatttt tagttcgcca ttattcttgc
    11101 ttattggaat catttttctg ataaatttga cgatgctaat gcgccttagc gacggcagtt
    11161 ataaaccgtt ggaatatgaa gaaccggcag agatttacag tgggcggcac gaatctttcg
    11221 gcatattgcc gcaatttaac cgttcggcga tgctttttac cctgactact ttggcgattt
    11281 ttctattttt cggcggttgg caaacgcctt ttgagcctga gggacaaaat ttagcttcgt
    11341 tgaccgttgg acttattttc ttcgttaaaa ccattctggt tttcagcgct tgtctggcaa
    11401 ttaagtacca cttgccaatt ctcactaact ggcaaattat taagattaac cttagaattc
    11461 aaattccagt tctgactttg atctggctgg gtttgttgat cattaggtgg ctctgatgac
    11521 aaattttcaa tggatgcctg tcattttttg gttggcgact tttttgacct tgctcccagc
    11581 gggcttggca attacctcga cgaatccgaa aaacctcatc ctgttgaata tgttcactca
    11641 attgggtatt ttcctactga taataagtct gggagtcggc ttcccggctg ttatcttcct
    11701 cgttctggtt ttcccattaa atataatgat gctttatgga cataatttat tccccgaaac
    11761 aaataaacaa accgccagag aatcacgccc ggcgattatt ttgaaaaccg ccgtatgctt
    11821 tttgcttttt ttacttttaa ttatagcatt ttatcaaaac catctgaaaa atatgttggt
    11881 cattgataat tcaggaaaga ttctggaagg cgttatccct gacgtttctt tgttattgat
    11941 tttgataggg atgtttataa tgttaatcct aatcgctatc agccattttt taaggagaaa
    12001 atgagccacc tttttttata tcaaattgtt ggtattggac ttttcattat cggtctatat
    12061 ggcttgtggc gcaatcgtca ttgctgggca gtaattactt ttttgcaatt aatgtcaagc
    12121 ggcatcttac ttagtttttt agcaattact cggacaaaag gcgcttatca atccgtgtca
    12181 gaattgattg taatatttat ttttgcggtt ctgattattg aaatactttt ggtaattgtt
    12241 tttacttata aagttcttat gtccgactca tcttcaaatt ccgacgacaa atccaatcga
    12301 ggttaaaatg aatattttga tcattctact tttattactt ccgatttgcg cctatttgct
    12361 tactcgatgt ctgccggata aatgtttatt gaatcggcgc gggctgtcaa tcctattttt
    12421 actgatttct ggattgatcg ggcttggaat atttatcgtt ctccttctga taaacatgcc
    12481 cgattacgtt caaaggattt cttggacttg ggcaaatatt gccggtcact cgctccagat
    12541 ttcattattg ctgaataatt ttttaggttt gctattagcg ctttgggcgc ttggcagttt
    12601 aatttttgcg attttgactg ccaccgattt ttttagcccc gatcattttc gtgaaaatat
    12661 taccaatctt gcgctgggaa ctttggcggt caacggtttg attttagcca atgacttttt
    12721 atcgttaatc ttcttctggg cgttaattga tttaattatt tatcgagttg ttttcagaaa
    12781 aattaataaa gcgtccgaat catattttcc agacattatg gtaatcggtg gtcatttatt
    12841 actgattctc gtcacctttt cactttttaa aatcagcggc acgtttcgat ttgatctgat
    12901 tatcgaagaa gtattatcgg gaaaagttga cccacagcaa ctaatattgc caggcagtgt
    12961 gctcattctg gttggaatcc tgcgcattta ttcgcctttc tttcgttatc agtttaatag
    13021 tcagacgaaa atgtcgatta gtgatgaatt catccttttt tgtggcttga tgccgtcggg
    13081 tttgttcctg gtgttaaaat tattcccgat tatgccccag atatttttaa ctggcttgat
    13141 gatattcagc gggattagcg tgttaatttt tcttataatt catttagcca agaccggatt
    13201 taatcaactt ttacatctca atacgatatt tctaatcagt ttgctcatca ttacaatcgg
    13261 tttgggaact tttccgataa gcgcttccct cctttttatt ctttttatga ccaattttat
    13321 gttaattatt ttggcacatg atttccaacc gatgtctgat ttagccaatt tttcaattgc
    13381 tttgcccttt cgtctgattg cctggttaag tctgattggg ttgccgattt cattggcttt
    13441 caacaccaga cttttgcttt tacaggcttc attaacatta tcatcaaaca agccgattca
    13501 cggaacgatt ataagcgccc tattggtttt gaattatttc ctaaatgttg tggtagtgtt
    13561 cagaattttt ctaaagtcag gctggtatga accgaaacat cgtaaaattg atcaggttgc
    13621 gccgctcacc cattgtataa tcgccggtat tagtattgtt tgcctttatc tattttttac
    13681 cttgcctaat tttaatccac tgacaactga actgaggttt tttccactat tctcgatcca
    13741 gggcagtcaa ccgctcgatt acagccagtt tagttcaaac tcactaatca tttactctgc
    13801 cattctgtta attggcttag ttgtcggatt tataacggca aaatatttca aagccgagaa
    13861 attctctcgc tggctaactg aatttagcct cgatttccga aggtatagta tgataccggt
    13921 agcgaatttc agtcaggtag ttgcgcgaaa tattaggcat ttcgatacgg ttttattctc
    13981 aagagtcagc caaagtttaa tgcaaacagc ccaaacggca atcctattta ttaatcgaat
    14041 taatgcaatc ggcgctcaac aaataaccaa attgaaagaa gttcctgaaa agtatttaaa
    14101 tagaatttta gatgggatct ttaacctgaa attccaggtt ttaatcggaa tcgttttaag
    14161 tatattaata attaccgttt taatctgtat tctttaagtt tttttccgga ggaagtgatt
    14221 tatgactgaa catctattat ctatcaattt attgattgcg ctgatcggct tgctaggaat
    14281 tgcactgaca aaacgcgaga aaggcaatct aattaaaact attgccgcca tcgttaccgg
    14341 tctgcaattc tggaccataa tagaaatttt attcaatttt aatccgatgg ataccgccct
    14401 gcagttttcc gaacggatta attggattcc agccttccga attgactatt atctcggcgt
    14461 tgacggcatt aatctggttt ttctaattgt tacttcccta ttgattttct taagtgtgat
    14521 aatttcctgg aataaaagag agcccctaaa agagtatttt attctgttaa tgattttgga
    14581 cattggttta aacggaatat ttatagcgat aaacttgttt ctatttttaa tttttcttgg
    14641 ggttactttt tttgctatct atttgataat agggtttagt aatatacgag gaagcgttaa
    14701 ttcggttaac cggttcggaa ttccctttct gataagttat attttcattt tgctcggtgt
    14761 actactcctt tacaagagca ataatctcca tactcttaac ctgaccgaat taactgtcgg
    14821 caacgcgatt tctcacagca aacagatggt gataagtata attttattaa ttggtttcgc
    14881 catcttgatt ccactttttc cattccattc ttggctgatt ccattaatta aaaatgggcc
    14941 taaggaagtt agcctattga tcttaggaat agtgaccaaa gtcggcttgt atggattggt
    15001 ccgtattatc gcgccggtaa tgccattcgc taccaactca ttgaccctgg cgcttggtat
    15061 ctggggattg gtaaatataa tttacggagc aatttgtgcc ctcgggagcg atgatagcaa
    15121 tcagattctt ggttatttta ctcttcaaca attaggattc tttttaatcg gttttgctac
    15181 gctcggcgaa aattcaaaca tttcgatagc agtgacgggt ttgagtggta gtttgctaat
    15241 cgttatcagt cattcctgtc tggcgacttt gctattaatc attttcaaaa atattcgagc
    15301 ggaaaaccag aacgttgccc ggccatcact cccaaatatt gcgatagtaa cgctcacgct
    15361 attggctggc attggaatgc caggtttttt agattttttt gctcgctttt taattctatt
    15421 gagtgctttt cagactcaga gattgttgat attggcgatt attagtttgc tcggcatatt
    15481 tttaaacttc gccatttatc tgaaactgtt caaaaaactg gtttttagcc agcaaactcg
    15541 cgatagcgca aaaaggttag attcgaatga ggtgtttgcg tcggtcagtt tgctgatcgt
    15601 tctaatattg tgcggcatat ttcccagttt tctactaaac atcatccaac ctggaattac
    15661 tcgcctgctg gaaatgttca gcacggcaat cgcattatag tctaaaaatg tcaaattttc
    15721 agtctatcat ctatttcata ccggaaatta ttcttattat tgcggcgatt ggatttttat
    15781 tgctgactac tttttttaaa atatctgatt ctaaaaaata ttttctgatc agtgttagtc
    15841 taataatttc attaattgcc acccttttat cgcattcagc tgacggacat agtattttca
    15901 ataatctgct ggtgtgggat tcatacggac aattcatcaa aatttttacg cttataatag
    15961 cctgcattat tctcataatc cgccgtaagg agaattgtaa tgaagccagc ccgagcccgg
    16021 tcaccgagtt tggaattgga cttttgctcc taattagtgt ggcgtttgtg agcattaatg
    16081 ccaatcatct cgtcgctttg ttcatatcac ttcaaattct gcacctcgcc ggttttttta
    16141 ttgttcagag tcggaatctc agtccccgga ttaataccgc cagttggcac gatttaatgt
    16201 ttgacatctt caccagtttg ataatgttaa tgggaatagc gctaatttat gataccacag
    16261 gtactttttt cattgctgaa atcggccaaa ttctcagcac tgtgactcgt atgaatttcc
    16321 ctttgatatt aggaatagcc ctgttattag ttggattttc ctttactatt actggaattt
    16381 atcgcttgag ctggaaatta gagatattgc ggacgatttc cgcttctgat aggctattgc
    16441 ttttaacgat tccgcaattc atcgaaacca ctaccttgat acgattactc agagtcgggc
    16501 tcggattggt cggtgattcg cgtcctctaa ctttaatgat aggaattttc gccgttctaa
    16561 cccttttgtt tccgattatt tggctaatca aattacgttc ggcgaaggat ataattatag
    16621 ttacttcaat agctcaaagc ggtttcggac tgttagcctt atgcgccggt aatatatcgg
    16681 gagatgtagc cgcattgttt tacgaggttg tcttgatttt cagtttgata gcattcacga
    16741 tcggagaaat tttaacgatc aatgcaaaat ccagccagtt aatcgcatta gcgagcctaa
    16801 tcggtattcc attaaccgcc ggttttccag ccaaatatct ggtttttatt agtctcgttc
    16861 agcacagtcc attctactat tttttaattg tactcgggat cggtagtgcc gcattgatgt
    16921 taataggtta tgtacgtata gtaaggcaaa ttatcaccgc acaagggcaa tttttcaatt
    16981 caccgcaaaa aggcagtcag ctgatatttt tcatccagat tctgattaac attatattaa
    17041 tttcaatcgg tttttactgg acgcctctga cggattacct ccaatccata atgactttct
    17101 gcgttcctta atttcgtttg accaacatta ttttactgat tatttctatt gatcgtatta
    17161 tattaaaatt gatgtattac tagttaaata aggattacta tgtcaccaca aaataaacgc
    17221 ttttttttct ggttcgttat tgcgtttcta atcattttca taattttaat atcaatttct
    17281 ttaatccata atttcaataa atccaagata atcagtttac cagcgcagga gatgcggggc
    17341 gtctggatga gtcgcttcga ttacactgaa ccgtttccat catacaatcc cgattcgatg
    17401 aaaacttata tcagtgattc ctttcgccgg ttaaaagatg ccaatttcaa tgtaattttt
    17461 ttccaggtac gcggcaacgc cgatgctttt tacgcttcac gttacgaacc ctggtcgaat
    17521 cttttgaccg gtaaattagg acagaatccc ggttgggatc cgctggctta tgcaattgaa
    17581 accgctcatc agctgggact cgaattgcac gcttggctta atgtttttcc agcttggcgg
    17641 ggaaccgctc ccccgactac ttctcaaccg ctccatcctt atttggctca tcctgactgg
    17701 ctgatatgtg atggaaatgg taaaaaaatg cttctctcag atggctatgt cagtttttct
    17761 ccgggaatcc cggccgttca taatcattta ataaaagtaa tttcggatat cgtcagtcgc
    17821 tatgatattg acggaattca cttcgattac ctacgctatc cggaacaatc ggaactcaaa
    17881 ggatattcac acgatccaat tagttgggct cgtttccaat cacatgaagg caatccactt
    17941 ggtctggact ggacagattg gcaaagggaa cagattaacg aatttgtagc ccgcgcctat
    18001 aactgcgtaa ccgacattaa accggagata aaagtctctg cggctgttat cggtagctac
    18061 aagaccgatc attggaatgg ttattcagcg ggatttcagg acccacggcg ttggcttgaa
    18121 attgggaaaa ttgatttaat aattccgatg acgtattatc accttaatca caaaaatttt
    18181 acttttaata aggctctcaa agaatggcaa tcagtgattg ataacccgga tcaaatcttc
    18241 cccgccttag ccgcatacaa tctcagttgg gaagaaataa ttgaggaaat ccatttagtt
    18301 cgtaatagcc atttcaaggg catggttttt ttcgccgcta gttcgctcga tagcgaaaag
    18361 ctccaatcac tgcaaacaac tgaattccaa tatccgtcta tcttcccatg tttgacctgg
    18421 aaagattcgg tcgcaccgcc gtccccaatt aatttcgcag tggaaaaatt aacatcagat
    18481 tcattagaat ttatctggca aatccctcat caatccaatg atattaaaaa atttgtaatc
    18541 taccagtcaa aaaacctgcc aattgattta acaaagggtg aaaatatttt ggctatcgta
    18601 gcgggtagcg atacttccta ccaaacgaag ttcaatctac cagatcagcg aaggtattat
    18661 tacaccataa ccagtctcga cgccgcttat aaccaaagcg aaccggcttc ggtcattaaa
    18721 atcaaataac ggattttatc agtcggagca tttttatcac atttccagat aaaatatgtg
    18781 acccagatca tagattgaca atggcttcga ctctggtaaa tcagattttt tattgcaata
    18841 ggacatatat tcctctaaat tttacgctaa gaacataaaa tatgaattac tattataaca
    18901 tacgactggg aatattttat agcctctatg ctgaaaggat tcttgatgaa agttaaattc
    18961 tggggcgtac gcggctcgat tccaactccc ccaacttccg aacaaatccg ccataaattt
    19021 ttaagaataa ttgacaattt accaaccaac attcggaaaa atccaactga aattaagaaa
    19081 tatattctca gcttgagcta tctagaagcc gggctaatcg gcggaaatac ctcctgtgtc
    19141 gaaatacgag ccgataacaa aatattaata tttgatatgg gaagtgggct gagagcactg
    19201 ggcaattatt tagtaaaaaa tgaacaaaat aaaaatggtt tggatttaca catttttatt
    19261 tctcacactc attgggatca tataatgggc tttccctatt tcacgcccgc ttttttcccc
    19321 aataataaaa ttacctttta ctcgccacat cccgatataa aagaacgcct cgaaatccaa
    19381 caagattccc gtttcttccc ggtctcgctc gaacatatgc tggcgaaaaa agaattcatt
    19441 caattgcaac ccaagacttc tattacatta ggaaaagtaa aaatttccaa ctacccgtta
    19501 tatcacccgg gcggctcgta tggctaccgg attgattggg atggaaaaag cgttgtctat
    19561 gccactgatt cagaatataa aaacctcgac cgtgaatcta ccaaacacta tattgatttt
    19621 tttcgcgggg caaatttatt aatcttcgat gctcaatata ctttcgaaga agccatccat
    19681 aaagaggatt ggggacacag ctccgccctt attgcagttg aatttgccgt cgaagccggg
    19741 gtaaaaaaat tggctctatt ccatcatgaa ccggaaagag atgattttga aattaatgat
    19801 attctcaaaa aatcacgcga tttcaagaaa ataaatttcc caaacgaaat actggacatt
    19861 ttcctggcta tggaagaatt ggaaattact ctataactgg aatcctatta ttttaatttt
    19921 tataaaatct tagagttatc ttaacctgct aatttataaa tccgtataaa gctgttgata
    19981 cagtccggtt ttgttatttt atagatcgga atatgaaaat attggtgaaa ttgcgaagta
    20041 agttggaaaa tatgatcaag aagcggcgtt aaaaactttg agcatccgga gaaaatagaa
    20101 gatgacacaa ttcaattggt ttgttttagt tattaggtag aatattaaaa tccaacaatc
    20161 cacctcatta tttttcaagc gcgtacattt ccggtgttac ttgataaatt cttagcggtt
    20221 cacggaattc aaaggaattc tccatctcca ttattttttc atcagaagtg agtattttca
    20281 gcatgggttc attaagtttg gtttgaatgt ctttcggatt tacctgggta gggtcgaagt
    20341 atatcaaaag cgcttcgcga tcagtgtagt cagttcggac tctaacaacc ccgtcgaaaa
    20401 aagaaatatg actggttaaa tattttaatg cccgccgcac ggcaatgctc tcggcatcag
    20461 gcaaaccaat ctcaaaaatc attaaatcag cgggtcggta cttctcgtaa ctattgaatg
    20521 cctgatcata tccagcgaaa agccgacgcc ggaaagtaac ataatcaagg gtatcaacaa
    20581 aagcgccccg cccttcacaa cggaaattga ccttcacgac ctcggttttt ccgttaggca
    20641 attttttctc gtaagatttt tggttgatca aataaataat tgagtcggga gttactttgc
    20701 caagttcgac atatatcctc gctatgactg gttcgccgaa attagtctcg aaaccgacga
    20761 ttttaggatt gacgcgcaac atacggatta ggtcggcatt atcctgaaca tcccataatc
    20821 cactaattgg aatttcaaaa acggcgattt tttcaggctg atattcgtcg aatttctgga
    20881 ttttgtactg ataaggcgta aaaatggctt ttttgatacc atattcgtca attttggtgc
    20941 tgtcatagta cacaactact ttatgacttt ttacgtaagt atccaagcca acaattcccg
    21001 gttgcttcat taatttattt tgaagtgatt tggaactgcc ccaacattta acggatttta
    21061 aaccttccat ttcaaatttc tgaactgatt cgagctcggc ataatttccc caccgctccg
    21121 agagtgtctt aaactcaaag ttacgggctg ttaataaagc aaggataaaa agtaccacta
    21181 aagctactgg aggaagccag ccataatttc ctttgttaag cctaatactt ccactactgg
    21241 agcattcttt ggtgcaatct aaacaaagtg tacaatccgg atgagtgact ttctcgtaac
    21301 tactgaccgg aataccttgc gggcagttat cgtcgcagtt accacaagtg gtgcaggtat
    21361 ttttattaat attaatccgg aaaggcgaaa ttgagaaaaa tttaaaacgt aggatttccg
    21421 ttaatgcgcc ggacaggcac aacgccaata ataaccacag gatatgcaat ttgataccaa
    21481 aaatatagag accaaaataa atcatcagga tgggaaccgt tattagaaca ttactaaaga
    21541 tattgctaag agctcccaga gggcaaatat atttgcacca gaaaaatcga ataaaaacgg
    21601 atagaactac aactgtaaaa atagtcaaaa taccaaccaa aagtaccgta tcactgccaa
    21661 acccagtaac tgcggcataa tagggatcga attttttgca ccacagttcg ctggcggtag
    21721 cggtaaaata ggctgtgaaa aagagcaaaa cgtatttacc gaggcgcaga atccgatccg
    21781 ctttcccttt cagaacaata gaaaatttaa atcgggcaaa aagtttattt aaccactcgg
    21841 ttactgtgcc aatcgggcaa atataaccac aaaataattt gccaattaga ataacaccaa
    21901 ttaatagaaa aattcccata aatacctggg tctcattcat actgcaggac atcgaaccga
    21961 gatgaaattt actcccgagc gaaactaagc caccgaaggg acaataagcc tcgaaatcgg
    22021 cttttttagc tttatcagtg catccgatga tcactaaaat aatgatcaat aacaggatta
    22081 tagcctgaaa caacagtttg gttttattat ggaaaaagtt cctgcgcatc taaatgaatt
    22141 ccttcattaa ttttctggaa atagtctggc aaaagtaagt agcccgcact ctggtaagaa
    22201 aggactgtcg agacagccct ttcttatcat aattgcccaa ttagtttatt tcacaatagt
    22261 catcttcttc acatttctgt aatctccagc ctgtatccgg taaatatacg taccacttgg
    22321 caagttgctg gcatcaaagt ttacgtgata ataaccagct ttcagaaacc cattttgtaa
    22381 aacggcaacc ggttgtccca gcatattgta aaccatcagc gtgacctggg tgctctttgg
    22441 taaggcaaaa gcgatcaccg tagtcggatt aaatgggttg ggataattct gccgaaggct
    22501 gaattctgtt ggtaagccgg aagaaccgtt cgcaacgcca acaaacgata gagcagtcgc
    22561 tccttcatcc gcacccatat aggtgacatt gggtcgcgca tcgccatcaa tgtcggttga
    22621 caaccaatcg ggacgatgac aggctaaatc gttgtcaccg atagaactgc cagtaacgtg
    22681 taaatcagtt gccgatacga aattgaccgg tttattgatt gaattggcat cctgaccgga
    22741 agcggctctc caggcggtca ggtctgcaca tggcgtcgaa ccatagaatc cgaccgaagc
    22801 actggaacca gcaatataaa tattgttata attggaagct aatccactat tgggcgaaac
    22861 cgtattgtgc caatgaatgc cgaatgaatt ggcggcgtcg tgacggttgg aaaggatatt
    22921 gttatagata tgtaacttag ccgaaaaggg aatttccgtt ccaattgcgg cgtgaacccc
    22981 ggtctgattg gtgggcgcaa tatcaatcgt attgtggtag atattagctt cgccactcca
    23041 atttgccgaa ccaaatgcta ttccataaac tcgattatca gtggcagagc cgttattagt
    23101 agtattcatc gcgataaaat tattgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23221 nnnnnncgat tatcagtggc agagccgtta ttagtagtat tcatcgcgat aaaattattg
    23281 aaaatatcta tgacaccacc gttttcacca ataactattc caccagcgaa ttgaggcgca
    23341 gttgtgttca tttcaacatt aactatgcga ttattaaaaa ccacacaggt gtcgccagcg
    23401 gcaaatccgt ttaagtacat accgctataa aatgtttgat cggcaagcgg attgataata
    23461 gaaataacat tgttgatata ggcgtttttt gagatgtagt cagccattat accggtacca
    23521 gcggcataga tatcacagcc cataacgaca gcgccgcagg gagtttcagg cgaattgccc
    23581 ctaagcgcca ctccgttagc gacggttttt tcaggagtcc cgatctggca atttttaatt
    23641 aaaagactat taggaacacc ggtatatcca tctacgccca agattgaacc gcttgccagc
    23701 agattttcaa aagtgatatt gacgttcttg acggtgatga aatcggaatt gtcgatcacg
    23761 cctattacac acttgccatc agtatcgtta ccaataattg tcaaatcgcg agaggttcca
    23821 ccgctggtat tactgccatc aatagtgaca tacgcggtat tttcaatagt aaaaccttgc
    23881 ccggagtaat taccggtagt tgggaaatca ttgattgtaa tggaaggcgt tttgccggaa
    23941 gccggtttaa tcgtcaacgc agtttcggct gtgagatctt cgcgatcgat gattaattca
    24001 gagccgggct caatcaagtc ggcgtcgata taaaaagtaa ctggcgctat ggcaccatag
    24061 gtattcagtg ccgtgacagc cgcgcccaga gtagcaaaac cctgttcgtg atcgccctga
    24121 gggatgtaat aatcacccgc cagaacatta gagtagatga cgtttttcag gatagttcca
    24181 ttttgaccaa ctgcccatag aatacccaca ctgccattat cactgtagcg gactttttgc
    24241 agaacctcat ctgttggact ggtttctacc gtccaattca gaccggcatc ttcagacttc
    24301 aaaatagttc cgtattgtcc gacaacaaat acggtattgt catcaacaaa ttccacatca
    24361 tatagttcag cgccattact ggcggtataa accaaagtag gcatagcatc ccaggcggtt
    24421 gagcggtaaa tatagccgga ggctcctgga attataccga tgccgttgcg aaattcgacc
    24481 gcccgcaagt ttttagcttc actggtcgcc ccaagtttgg taaaggtcat caaatcagtc
    24541 gaagtataat attctccact agcgcaggtt gctacgaccg cattgttgga caatggccag
    24601 atatcgtaaa ccgttttgga accagcggta gctttggtcc aagtgtcccc accatcgaag
    24661 ctgtaacaaa tatagccgct tccaccccct acaaaaaccg tatcggaatt taggtaatgc
    24721 atagcattaa cggctttatt gttattatac atcgggttat caatctgagt aaaagtagcg
    24781 ccaccgtcag tggttttcaa aatgtatcca ccactgccac ctataagtcc gttattagcg
    24841 tcaataaatt cacattcaaa aatatcgccg tcgagaccag tcctcagatt gttcggataa
    24901 gaccaggttt ttccccagtc gttgctaaag gtcatttcac caccaccgtc tgcagttaat
    24961 acgatttttt tctcgtccgg gaaagccaat cccaaccagc tgattccagg ccaattatta
    25021 ataggaatcc aattcacccc accatcagca gttttcatcc attgctggta ttcgcaaaac
    25081 cagtaaccgg tacgggcatc aataaaatca gccgagagaa gcctttgacc tgttccagta
    25141 ctaaaattag tccagtccgt tccaccgttg gtcgttatag caccaaaatt atcagagcca
    25201 ccgccataaa taatggtatt attatcgaat acctcaacat aagaggtata gacattggca
    25261 aattgaacaa cacctttgtt cgcaaatgtc cggccgccat ttgtagatac cagacagacc
    25321 cgatgataac aggctagagc tacggttgaa tcaccaccga ccgcgacatc atagacccga
    25381 taagaagtta caccaggcgg aaaagctgca gctattttca ccggatgcca gtttgtgccc
    25441 ccatcctcag tccaccagat agtcgcttct ttattaaaat ccacggcaac tactccgcgg
    25501 ttttcatcga aaaaggcaat accaccatca agatcatcgc gggtttcatc actggcggtc
    25561 atatcgccat cgaaattgta ggaaagcttg gtgaaagtgg aaccgtcaat agtttttaga
    25621 accgtaccgc ttttaccggc gcaatagacc acattggcat tgaccacatc gagactatac
    25681 agatcgaatt tataggtatt agtatcgccg atcatagtcc aatccgagcc gccattggtg
    25741 gtcttgtaga ttacaccacc accgccgcag gcatagccca cattggcatc aataaactcc
    25801 atatcattcc aggaattggc ggatacatcc ggctcacgca cagtcgtcca tttagcacca
    25861 ccatcagtag ttttttgcac cttgcccatt tgtttgcttg gataggttgt actatatact
    25921 atccagccgg tattagcatc aataaaagac gccttgttaa cggacatatc cgtcgaggca
    25981 atttttacgg tctgccaaga accgcctaag agtaggttcg caacagnnnn nnnnnnnnnn
    26041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26101 nnnnnnnnnn nnnnnnnnnn nnnnnncgtt caattccaat ttcgcaccca tagcgagttg
    26161 cccgctcagc attccaataa aaatacaaat catcaatcgc cagatttccc agaaaataga
    26221 ctgacaaaat ctgattgatg gcatagttta aatcgaggaa ggcagatgaa tgatagtcgg
    26281 tctcgtggta ttgaaatgga atagtgtatt cttgatagtc acaagcttga ctggcgtaag
    26341 aggcgccgat attcattttc agccgcttaa aaagccgagt ctgccagcgc aaagaaaaga
    26401 cattgagcaa ttcggcatta tccagaataa tgacctcata attgccagat ttcacccaat
    26461 cgtccgtaga gcggatttca tagataaatt ccagggaatt attcgattct ataggggaat
    26521 aattcaaatc aaggattccg cgccggattt cacgtaccca atgaccacgg ggcgtgggtt
    26581 tctgaggact tccagcggtg acattatccg aacgtccgtt atattctccg gtaattatca
    26641 aagccaggcc aggaagcata aaatcacggt ggcagaattg caaagccagc ccgccgccct
    26701 cacgttcatc attttttcta ttaatggatt tcggagtttc atccttataa atatgataac
    26761 caaacagagt gcgtacgaag gcatcttgat agtacttcac agcttcatat ttgcgctgag
    26821 cgttataata ggtccccaaa agccccccga ttattttctg attcttcgaa atataactca
    26881 agccgccgcg caattcagta ttccgggcga taatctcgca ttgggtatag acatccttca
    26941 aactttgttc aactccgtaa ttaatctcaa gcccgagatg caggcgtttg gttaaggcat
    27001 agtcataaag aaaattaatt tgcgggctat tataagttgt cgtgccagtg gtcgtatcca
    27061 gaaaagcaat atattcgtca taaatatttt tttccaaaga gtggtagaga tcacgctgaa
    27121 tagtccgatt aaacctgata ccggtaacca gggtgttgcg ctcactgaga tgtttggcgg
    27181 aatgaaaact aagggcataa tcttcctgac gccgcgggtc atatagccgt ctaaagacat
    27241 tattatcatt ggtagcttgc atctgataga aatcgagatt atccgggtaa gaaatgccgt
    27301 agaaggcggg attgccggat aggtcataaa tagtgagatt atactgattc attaaattac
    27361 gcggctgaat ctcacgccca agcggattgt caatagcgaa taatggagta atgaatacca
    27421 gtccaatcac tactcctaca aataggcatt tttttataaa atcactattc gttctcatgg
    27481 tgatgggtga tgatatcctg gggtaggcgg tttaataatt acgaagtcgt taaaggaatt
    27541 attggtatca aagccaggca gaatccgctc aaccgactgc ccgctatact tctgattgcc
    27601 aacgccagcc caaccggcgt ctaaaatttt atcaatgttt ttggttgtct gttcgctagg
    27661 gcttttactg ttgtattcca cgcaatccac tacggtaccg atatcgagtc cgtcggtcat
    27721 aaccgagtcg ctaccatcgg cgagtgcgat gacgtttgtt gatagattga tcataaaatc
    27781 tacggttcta cccggtacga tattagctaa atttggaaca ttaagattat ctatgtcgcc
    27841 gaagtccata gcattgacaa attcccaatc ggctccagaa agatcaacgc tggtgctgta
    27901 taactccagc caattaactg catcctgggc aaggacgaca aattcacctg gttgaaccgg
    27961 atattctcgg ccggtcagcg gtgtgccagg aaagcggaaa gcataggtta cagacgtcaa
    28021 catatgataa atccggcaga cgatcattcc gtccagatat tgaacctcat ccgaggaatt
    28081 atacagctcg ataaattgat cgtaaaacca attattccga ttttcggggc cagtgtaata
    28141 aatctcattg attttcaagc caggccggag gttagcggta atggtataaa ttgtatcggt
    28201 gcctattccg gtttcggagg gagtaatcgc cacaatcgtg tcaatcagag tatagcttat
    28261 gagcgaatcc agtcttctat tcccaaaact atcaatgaac ggcaactcga ctttggcttt
    28321 cacctgcgga tcaaaaatcg cccagggcaa attttcaaaa accactaccc ctgaatcatt
    28381 agtagttgcg caataatcat caaggttata gtcgccagta tgaattttga catcgacctt
    28441 tgatagtctc tcatcgtaac cgagggtaga gtcccgatat accaatacca gttttaaagt
    28501 ataggtgcta tcgaattccg ttggtttctg gtgaaaacag ttcgttaaat tcaagagtaa
    28561 gataatggtt aaaagtgcag ctttttttac cattgagcac tcacttccaa tccgtaaaaa
    28621 atcggcggat tgtgttcgtt taacgaacca tcgaaatttt tccatacagc ccggtcatcc
    28681 aatatattat tcacaaacaa ggaaatttcc atactttggg agagcgactt ggtaactcgg
    28741 aaattgaaga gccaccgacc gccggtatcc cgcagataac gctcgtaata ataggtcatc
    28801 ccctgatacc acaagtaagt cacgccattt tcagtcttat aagtcgaatt gctgcggtaa
    28861 atacgcttga aatgttcgag aggcacctgt tgagcatcga gtgtaaccca gatgccgagc
    28921 cgattgctga tataattaat ctgatagtct atgatgatct tttcactcca gtatgactcc
    28981 ggcttgtacc aaatatcttc ccaattttcc gggttagcat catagaccaa gccagttttg
    29041 ccgtttttat tgaaccgata ggtgacattt aaccggtatt ggaaattgtg aatgcgctgg
    29101 gtgcgcatcg taaattcgat cccattcgaa cgactccagc ctcgattttc gaaacgctcc
    29161 caagccgcgg cggtaatcgt atccggatta atttcatagc cataaggata actgaccgta
    29221 accggcatat tgtccgaggc ttgataatag ccggtcaggg aaaaacctat cagatcgctg
    29281 aatttccaat cgagagaagc ctcatattta tcaatcgaat aagcctgtaa atccggatta
    29341 tactgcaggt gcacttcttc tatgattgta tcacctgagg catatttgaa ataatctgga
    29401 gctttaaaaa tgtgccctaa ggaaatatcc ttaacgcttc ggccggcgcc caagcgcatc
    29461 tgccaatctt cagtgattga gtactgcaaa ttcagacggg gacttagaaa atctccgtgc
    29521 tctgatttga gaaaggcttt ttccttgaac atatttttga aattgaatcc aatcggagag
    29581 taagcgtcat agcgtaaacc agccatcagt ttatagcgcc gcgcgagcat tttgccctcg
    29641 aggacatcct cagcatagaa actcagggac gtcaaatcgg gataatcatc gaaagcatag
    29701 gagcgatgcg aggaatattg cccgtaatag gggaaatttt cgtcaattac tacgccgtcc
    29761 ccggtgtttt tttcataatt gacttccccg ccgaataaaa gattatgctt tgtatgcttc
    29821 gttgtcaagg cgattttacg ttggattttt ccggcaattt tccattcttc gccgatttct
    29881 ttcatcttgc cgataaatcc cggcaaaatt ttaatcaggg ttgtatcaat cgcgattccc
    29941 gtgaccgaat ccacgacagt tgtatcatat ttaagaatat aattttccgc cacccatttg
    30001 gatttatagt ttcttttatt attaagatta actgagaaga cgcctttcca acttttagcc
    30061 tcgctatctt tataaataaa attgaaatcg ccggttgcca gaaatccttt atcataagaa
    30121 gccatctgat aaacgtcagt gggtttttca ttatcgagca acttggtaaa acttcccccg
    30181 atatgggttt cgagtttttg atctaagtat gcaccggata ggttaccgct ggcgtgtaaa
    30241 cgctgaaatt catcgccaac tttgcgcaaa tcgcgttcgc tatacccgta attaaggtga
    30301 taatccagaa tagagcgacc gagcttaatg ccatgtgaga agctagcgtt ctttgtatcc
    30361 ggattgattt tagcattgaa tttaggagca attcggccga tcttggtttc aactttaata
    30421 acgccgctgg caaaattgcc gtattcaacc gaggggatgc cggtaattac ttcaaccgat
    30481 ttaatattat cagccggaat gatacgcaga tccaggcctc tggtggcact cccgtaatag
    30541 acgttgccgg aagtgttagc gtcggttgag atttgattat cattaataat tattgttgat
    30601 ccaaaagact cgacggcact cattgataca ccagcggcgg tcaccgaacg caagccgatt
    30661 tcactgcggc ctgagagacc cggattctgc gatttgtcca cgccgggaac aagtgagaga
    30721 atatcgccga ggctggtagc ttgcatatgt tcgatttcac cagtattgat agtgcgggtg
    30781 gtaaccggcg actcagggat taattctttt tgcactgaat agacttcgat accgcccagc
    30841 atgaaacaat aatcgctgga ggatttgccc gcctgcttga gcagtttgat attaatctcg
    30901 taatttcggt cggcttcgat ttttagatcg aataacacat ttttgaaata tccctcgcgc
    30961 acagcttcga tattataaac acctggttca acatttgtga gcgtgaattg accggtctta
    31021 tcggaaaccg ttgtacgtga aaaatccgga tcggaacgtt tttgcaggat tactaacgca
    31081 tccggaatcg gtttcctctc catctggcaa gtgactttcc ccgttatagt tgaggcaatt
    31141 ccccgaatcg ctaaaccagt caggattaga attattatca cctgcttttt catattcttt
    31201 tagcgattca tttgaattcg tttagggcgt cgggcgccat attcccagaa actgcgatga
    31261 tgtaattttg agtatcgaga tacttattgg ctacccgctg tatatcagct tttgtaacgg
    31321 caagaatgcg ctctttacta tgatcaaagt aatcaagcgg cttcttctcc tgaattaaat
    31381 cgaaaatgac cgcaccaatt tcgtccggtg tccgagtatt catcgttagc atagcaattc
    31441 ggcgggcttg ggctttttcc agttcttcat cagtaactcc ctccctttga accttctcaa
    31501 tttcagcgaa aatgccatga atcattttac taagattggc ttggtctaat tcagtacgaa
    31561 tattccaata accgccgtga tcgcgaatcc agagattgct tttgatacta taacttaagc
    31621 cttgtttatc gcgtaattct ttccccatgc gggaggttag cgaacttccg caaaggattg
    31681 attcgagtag tttaagagct tcaatatcgg gattatctgt tttgatgcca ccttctactg
    31741 gacaaaaggc gatatctact cgacattgtt tgtatcccgg attgctaaaa acataaacgg
    31801 ttttaccctg gatcggctta accgtctgga acggcaaatg cttatctccg gatttatgtt
    31861 tccaactgcc ataagtggca tttaagcgcg aaagcatttc tttgcgattg aaatcgccaa
    31921 ctaccaataa tttcagattg tcaggtgcat aatatttggc gtgaaatttt tttaaatcag
    31981 cactggtcaa aattctgtag gtttccggat ctccggcgta gggtaaagca tactggtgcc
    32041 cttcaaaaac tttttcgaac atagcgtaaa aggctttcat acgatcggtt tctttatatt
    32101 ccttcgcggt tttaattaac gacgggcgaa ctttttcaat ttccgattcc ggaaaagtag
    32161 gctgagttac aatctcaaaa ttagcctgta gaattttatc tacatctttt gagagcccat
    32221 aaccctgaaa aataatctta ttccagctct gactaatatc aatctggtag ggtatgaacg
    32281 agcgctcttc gatcaattct tcataagtgc gtttagcggt gccccgcgaa agcataccta
    32341 ccgtcatctg gcgaatgcca gggtgtaaat tttcctcttc gagccgccct gtttcgatat
    32401 atccgattag caaaatagtt ggatattcat gattttctaa aaaataaacc ggcacgccgt
    32461 tttttagctt agctgtggta accaatggtg cgatcggatg cggtgaaatc acttcatcta
    32521 acttaggaaa ataaatctga cgcacttccg gattgacata tttaccggta gtatcttcag
    32581 attcttctat cgtttccggt tcggttattt ccagatcaaa ttcactttcg tcactttctg
    32641 cggctaagaa tccgactttc ttttgggtag tatctgggtg ggagtaacag gcgacataat
    32701 tgccggtttt caaatattta ttaacgaccc gtataatgtc atcagcggtt accgccatga
    32761 cgagctgatt ccattgattt atataacgcc aattataaaa attatcataa gttgcaagtt
    32821 gcccaccgat tttttcatta tgctgattgc cagttagttc attaaagacg atttgatttt
    32881 taattttttg aagatcataa tctaagaccg ccgtcgtcgc caaactgtca atttccttcc
    32941 agattagatt ctcaacttcg ctgatttggg cagttggcat caagctggtg atcagattaa
    33001 aagtcgaggg gtcttttgta aatgaaagcc cggctaccgc cgattcacaa acctgcttct
    33061 cgcgtttcag ctttttatta agtcgcgaag tcgaactgcg ggcgcccaga atctgcccgg
    33121 ctacgtataa agctggtcca tcagcttcaa aacgggttgg cgcttcaaaa taataatgga
    33181 ttgattcgct taaaatatcg ggatgaatga aatcgagcgt tctcttaccg caaattggaa
    33241 cacgataaaa attatacgcg ggaatctccg gaccgcgcgg aattttacca aaataggctt
    33301 cgactttttg cataatctcg tccgtattga agtcaccggt gagaacaagc gtggcattgt
    33361 tgggagtata ataggtgcga tagtatttat aggcgtcatc acgattaata ctacgaatat
    33421 cttccatcca gccgataatc ggattgcgat aagggtgatt tttgtagatg agagtattaa
    33481 tctcctctcg acgcaatcca accgcgctat tctcggtgcg ttgtttgcgt tcttccatta
    33541 taaccttgag ctccgattcg aattcttgag ggtcaaaagc acatttatac atccgttcgg
    33601 actcgatgtc gaaggctaaa tcgattttat tttggggaag taattcatag taaaccgtga
    33661 tgtctgtgga ggtataagca ttgaatatac cgccattagc actaatcagt tcggaaatag
    33721 tacctttcgg aaaacgatta gtgcctttaa acatcatatg ctcgacgacg tgacttatcc
    33781 cggtcttgcc aaacggttca tatttcgatc cgacattgta agtcaattgg gaataaatga
    33841 ctggtgcggt gtgcatttca tacgtgatga ctttcaatcc gttatttaga gtaaattcct
    33901 tgacccgtga ttcatcgaga gccaggagac tcgagaaaat aattaatatt attgcaattg
    33961 tataggctaa actacgaaac catttcattc taaattgttc cttttttgat ttaaatgttc
    34021 actaatattc taaaaaactc aacgaatata aaataatttc tacataaaat caatgaagtc
    34081 aaggaagact cgggggatat gatttgctat atttcttaga gaaaaattga tagccagaat
    34141 tcagcaatag attgttataa gggcaaaata aaatcggaaa ttagtggata aagaattgat
    34201 tcaattctgg tatgaaacac taaaaaatat ggaaactatg agtgtccccc taaaatttta
    34261 attgtttcct ttgtcagata attatcttgg caaaaagata atatttatct aaattttcgt
    34321 ctcttttgaa attagatgtt ggctgtttga aataataagt gagtctgttg tttttccagc
    34381 atatccggga gctgaagcgt aatcggcttg tcgggaacgc tcgactgtgt atggaatctt
    34441 tgccagcaga gatagtggcc gccgcagtat aatgtcggta aattaccaca cacagatcat
    34501 catacccttc ccacttgaaa aacaacctaa acaattcggg gatcgtccaa tggcaggacc
    34561 acggcctttg gagtcgttaa tctaggttcg aatcctggtc cccgagcaag tttttaaaat
    34621 gagcctgaat atgaaaaata atggaattat tttttgtgga aggtccaatc cccagttggg
    34681 ataccggatc aatgaaattc tcgggataaa acccggagca attaaaatta gtaattttag
    34741 cgacggcgag atcaccgtca aattggaaga caatatccgc ggaaaagatg tcttcattat
    34801 tcaatctacc aatccgccag ctgaaaatat tctggaactt ctgttaatta ttgacgccgc
    34861 ccggcgcgcg tctgcccgta gaattaccgc cgttctcccc tattatggtt acgcccggca
    34921 ggatcgcaag gaccagccgc gggtgccgat ttccgcccgt ttaattttgg atacgatcgt
    34981 cgccgccgga gccaaccgaa ttatggctat ggatttacat tcgacccaga ttcaaggctt
    35041 cgttaatatt ccctttgatc acctgtatgc caagcccatt ttcgtcgagc ccttgcggaa
    35101 gctgatcggc aaacgcgatt tctcgatcgt tacccccgat gtgggcggaa tcaaagttgc
    35161 tcgttcttac gctaaaatcc tcggtttgcc tataaccatt attgataagc gccgcccgaa
    35221 gccgaatatg tcggaagtta tgaacgtgat cggtgaaaaa gatatgaaac atgcgttaat
    35281 aattgacgat atgattgata ctggaggcag tattactgaa gccgccgctt tgttaaaaga
    35341 gtatggtacg gaaacaatca ctgccgtcgc cactcatggt cttttttccg gcgattgtgt
    35401 taaacgcctg agcgactcgc ctattgatcg cgtcatcgtc accgattccg tagcgattcc
    35461 ggaagaaaag cattttgcga aattgcaaat tctctcagcc gcgccgatgt tggcggaggc
    35521 aatcaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngacc ttaatattaa
    35641 taaaaaaagt cacaaatgca ttatacgcga tgttcagttc gacccgctta ccgaagagat
    35701 tatccacgcc gactttatgg gcgtcagcct ggaagagatg gtgaccgtca gcatcccgat
    35761 taatgtcacc ggtaccgcca ttggcgtaaa aacttttggt ggaattctgg agcaacatct
    35821 ttgggaattg gaggtcaaat gtaaagtgtc ccagattccc gatcgcgtta cgattgacgt
    35881 gactaattta aacctcggcg attcaatcaa cgccggtagc cttaatatcg agggcgtaga
    35941 aattctaata cccgctacga cttcgattgt ctcagttgtt aaaccgacag gtaccaaagc
    36001 cgaagaagaa gtcgctaaag aagaagtcac cgaagaagtc ggcgccgaag aaacggctgg
    36061 cactcaggaa gcgaaagaaa ctaaagagac caaaggtaaa actgagaaat aatgaacagc
    36121 tcaatgaacg gctacaaatg atcagtgcaa tcatcggttt gggaaatccc gggaaaaaat
    36181 atcagcacaa tcggcataat tttggccaaa tgtgtctaaa ccacattgct aggcggcata
    36241 atctggcatt taaaccagga ggcggcgagc gtgcctttac ttacgccacg actgtaattg
    36301 ctgatcggac agtctatctt tgccagaaca ccacttttat gaataatagc ggattagctg
    36361 ttcgtcagat tctgaaccaa ttcgacctgc agactgatga aatactggta gtttacgacg
    36421 atattgactt gccactcggt aaaatccgtt tgcgcgagaa gggctccgcc ggagggcatc
    36481 gcggtatacg ctctataatc gagacgctcg ccaccaacca gtttgcccgc ttgcggctgg
    36541 gaattggtcc gcaagatgaa ggcgttccat cggaagattt tgtcctgacg gattttcgtc
    36601 cagcggaaaa agaactggtt gaagccgtac tggaagaaag ctataagtgt atcctgacca
    36661 ttctaaacaa tggcattcga ccggcgatgg aatatttcaa tcgccgcgct ctgcaccttg
    36721 ctcccaaaaa aaatgaaatt caggtaaatt caattaaaaa accaatcata ttaacataag
    36781 gagttcggac ttgaggtact atgaaaattt
//
LOCUS       DAOOMU010000008        35190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_976_length_35190_cov_20.118998, whole genome shotgun sequence.
ACCESSION   DAOOMU010000008 DAOOMU010000000
VERSION     DAOOMU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 35190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35190
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_976_length_35190_cov_20.118998"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="PLI55_01460"
     CDS             complement(<1..881)
                     /locus_tag="PLI55_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPA99537.1"
                     /translation="MENTSGIDIGYQFSDKLTDDQTLATEISNASPAVQHFIADLCDK
                     IDNLSKIGAALSAEHNLPVLLQMILEEARRFTNADGGTLYLMSDDRKWLNFAIVQTGS
                     LNIKMGGTSGNPINWKPVPLYLPDGKPNHAMVSAYVALTGEIINIPDVYNVKGFDFTG
                     TRAFDKNTGYRSKSMLVVPLRNYEDEIIGVLQILNAQDRRTGEVCPFSSTDQKLIISL
                     ASQAAIAITNARLIHDLENLFESFIQVIASAIDEKSPYTGGHIERVAQLTMLIAHKVN
                     DVNYGKYADFFMNEDNL"
     gene            complement(917..4279)
                     /locus_tag="PLI55_01465"
     CDS             complement(917..4279)
                     /locus_tag="PLI55_01465"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPA99538.1"
                     /translation="MNDNKLTHKDWNLLAICGALLLISIIYLSRNYRKAFPAYDLKFE
                     ITRADSRDLAEKFLSHQHIDLNDYRYAVVFDYDELPKTFIEKEVGFEEATRLLCQDFP
                     IWHWSNRWFKPLSPEEIKVNLALNGRITYFEHFLPEEAAAERISVEAARRICQDFLMT
                     TMQMNPAEWEFIEDKTDIKPNRVDHFFTYQKKGVEISSATYRLEIGIVGGKVGKYREY
                     WKLPEQWLSNYKHLRSLNSTTALAADVLFVLILIAALVVFINQLNRKNVHLKTALGFG
                     LITFILQFAGNINELPITIYAFDTNQSLGNYYGNYFIQTFVIALLYALLVAVITGAGE
                     VLYRRAYPQHLSMSKLFSPTGLRTKSFLINSTLGMILAVVFIAFQTAFYLIANRFGAW
                     APVEVNYSNAINTYFPWIFVLLIGFIPAVTEEFSFRLFAIPFFAKIFRSKPLAILIPA
                     LIWGFAHANYPNQPFWIRGAEVSLFGIFVGVLFWRYGILTVLVWHYTVDAIYTAVFLV
                     KTGQPYLIVTAILAAGVIVLPIIYNIVCYRRNRSFADSSGLLNGETVFIIKPEKTITN
                     EVPEISEVKEYQSYSPRKIRNGLIISALFLLILLIPVHRVGDFYRFPIPKKEIRQTAQ
                     EFLAEKEIVSSDFKITMGLNNNYNPLMGQYVIQHSSLNQLNFIMAQFLPDIITWQVRF
                     FQPNQREEYRIYVHPMKNTVAAFSHILDETAPGARLSKASAQRKAEEFLTFEKYELSN
                     FSLIESNSDVLPNRTDYHFIWESRDEHPASVADARLRLTVTLKGDEIAAFATDYKIPE
                     DWLLAQTRQTTFQSIMTGVQIALIILILYRIIKILLNRPKKVFSGWRTAIRLALYLAG
                     LAIIVSGLNYSAALLNYDTSWTLTNWNFLWILSILLKAIGVSLLTVLIFSTVGLLYPD
                     AIQTLQKAHRPRYTRDALIASILIICGLLACNRLSNYIISMIKPELLANHQITSGLED
                     SLFPFFGALQRIIIMSLLSVATIAFIGFILQNLKGQNFRKVMTLIMLTAVFLPDNYLT
                     LSGYLVVYLQFILPILWLYFCLKYFVGANPPAYLYAAVGYFMINQIFSLLRAGSPQGR
                     LAAILLILLGLILSSWLIIEKQNVNFKSTMSN"
     assembly_gap    4355..4454
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4604..5809)
                     /locus_tag="PLI55_01470"
     CDS             complement(4604..5809)
                     /locus_tag="PLI55_01470"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPA99539.1"
                     /translation="MKVLTLNSGSSSVKYQFIDTSTRTVLCKGLVDRIGISGTSLTHK
                     RQDGHQVVITDNIVDHQTAINSIMDILIDPTHGVAQSLKEIEAVGHRLVHAGASFKQS
                     VLINEAVREKLYECIEFAPLHNPHNIKGIEASLKKLPHTPNVGVFDTTFHINMPDYAY
                     LYAIPYSYNEKYGMRRYGFHGTSHYYVANRAAEIIGKPIEQLNIITCHLGNGCSIAAV
                     RGGISVDTSMGFTPLEGLVMGTRCGDIDPGLILTIIDRENFSIEEANTFFNKQCGMKG
                     LSGLSSDMREIENAAAAGHQRARQALDIFCYRVKKYISAYIGVLNGADLIVFTGGIGE
                     NSALVREKSLSNLENLGILLDPAHNKIAHGVEGIISSAESKINVLAVPTNEELVIALE
                     TEKVIITSQ"
     gene            6193..7473
                     /locus_tag="PLI55_01475"
     CDS             6193..7473
                     /locus_tag="PLI55_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99540.1"
                     /translation="MKEINFKIDLANEYHNFNQFEAKLIKGIREKVCEQIVQFLEEWD
                     QELGTRLRQHYPQYRHKGYIHRQVRCYFGVIGIKMRRYVHKGWPDIYPLLFILPEDGI
                     SGELMDLMLELVTDVPYQRTNRYIKKATGVAISSQTIWRKVKRLGQDERDRQEQIRCK
                     IFEEGGDDYCQDYRQLPACQSGGKESDLVSPVYIELDGTMVSSREAGEERFEIKSGIM
                     YSQVVQMGEKRRRLMDKVAYGSVSDSETFGENFYAFCRENGLDLRREILFLSDGAQWL
                     NSVAEDVFPEAQKRLDLYHLKKACGIVLDEQELNTLSQAVYYKTGEEIIRLVQNLCSR
                     KNLSGKSVDELMQYLGNNQKAIEYPPGERHGSGGIEKNIGILVGRRFKRQGMSLTAIR
                     QVWSHEGANNLLALRAKKLNQIWQQESQALNYTR"
     gene            7589..10795
                     /locus_tag="PLI55_01480"
     CDS             7589..10795
                     /locus_tag="PLI55_01480"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HPA99541.1"
                     /translation="MKFKITLVFIICLVLSGMMNFGLAQSDQPLIFSFKQNHTVLYKI
                     AKCSNCDNDENFDVIGIAGLVDSHGQLVPRSTYLVHLEESENSDFTVQWSYNLPQDIK
                     GDFTDIAVINWNNVPAIIAVLNITEAGTGPDPGWLLLFEYSDGFSKEPTVRMTTDTVF
                     PIRPRPRYLSVGDLDHDEQSDLIISSSSPNRSINIVTQAADTSALSLKLINYSSDLPV
                     LLGLQPFRALAANVDTEPGDEIVIFGGQQKLTVEVYRSDLKNPLSSYTFSNVNRNDIN
                     LDQLAAGDLDGDGLAEFILPLRSGGAQLLVKGVQSYKSGLLIPKKNKIAALNLVDING
                     NGLDEILWADAAGADIYRYEYNPGANLFDLDTYISFHYSNPVLANVQLLSIAPVVSYG
                     GKNTGAIITPFLHRSFNQHGLCYWLLEETSPLIDESLIETILGSVDSVLAHKEFDQKK
                     GASSLTEMDLLKQQLGSLVGTEIELSPLPEGEGKPSGISGDRQIYQPDILLHPGELMR
                     YNLNLPDLSLDDTKKLSVNIDAPSGMKFDLANKLFTWVPADSQLGLHKVTALISWDEK
                     KDVQTFTVYVNSIPKILTKIPIRDIIQIGETFSLQIEVEDANQDAAIFYKLLEFPAGA
                     SINQKGELLWKPSFDQADWYDFIVEISDGYDSEQKSFALFVNHPVSIQSTAPKTTTIG
                     KKYTYQPVISDNNKGFYLTGYSLPPRIENWSQTGVLETQILDKNIRNNISKYIENYRK
                     SFPLFITPAKMNDVPQSLFQDIFIDSEKVVFIYNLRTAKGMEAGQIIETFFSNLKMSI
                     PKYSTPVRRYLYIFNLKEPIAGLTMTGEGLITWVPAANQFDYQLISYTVTDGFFSAEE
                     QAQVYVNYPPKITSKPVTIASVNTLWQYEVKVTDLNTDSKLTYELLSAPEGMVISPQG
                     IISWQPTDLQINSYNFTIKVSDGFAQVVQKVHLFVNVKPKITSVPKPVALTNLKYEYK
                     LEAEDANNDKLTYKAVRLPKYASFDPTTGMLVWTPRKDQIGANDIVLKVTDSHGGSTL
                     HEFQVHVFANPARRHSFLRRTISLLTIAGVIYLVVIYL"
     gene            complement(10790..11845)
                     /gene="lpxK"
                     /locus_tag="PLI55_01485"
     CDS             complement(10790..11845)
                     /gene="lpxK"
                     /locus_tag="PLI55_01485"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPA99542.1"
                     /translation="MQLGALRWLLFPAAVIYGLITKLRNLFYDRHIFKAIKVPVPVVA
                     VGNITAGGTGKTPFVIALAKFLSEKGYRIAIISRGYRRKSRGTVIVSDGRQILTTPQQ
                     AGDEPFLMAGKLPGVVIVADADRIAAAHTAIENFACNLIIADDAFQHRRLARDINIVL
                     WDPKTPSWQKYLFPVGRLRESWHGLKRADYLVFSKTDKIIPEQRTFFEKFNPSITFFT
                     ASLKIVGLRTLSAENVSINTIAGKNVLAFCGLGNHIQFETTVRQLNCGKVTFLRFPDH
                     HHYTTTDLQKIIIKAEQMNCDYLVTTEKDAVNLPVNAGSLAIPLLVIEIELKLSDEIK
                     TAILQRLPPSSISGADL"
     gene            complement(11861..12985)
                     /gene="lpxB"
                     /locus_tag="PLI55_01490"
     CDS             complement(11861..12985)
                     /gene="lpxB"
                     /locus_tag="PLI55_01490"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPA99543.1"
                     /translation="MSAGKLFISAGELSGDMHAGKLISALKELNPDIEISAIGGDNMA
                     AAGAELLFHIRETSFMGFVEVIKHLPFIISMYRKTLQFLDEFKPDVVVLIDYPGFNLR
                     LAKALDRRDIPVIYYIVPQVWAWHQSRVKKIRRHVQEVICILPFEATWFSERGVRATF
                     VGHPLLDQNEISIPEPNQKIFKNGEPLVGLFPGSRQQEVDKHLPLMISSVKDLRQEFP
                     NLQAAVAVASDVNLEKYQKCYQFDWLSWHFNSTRSVIQSADVLIMASGTVTLEATLSN
                     KPFVVIYRVAPLTYWLGKKLIKVPFISITNLIAGKKGVTELIQHDANVENIKSEIMRI
                     LCDQSYANQIRDFLQYVCRLLGEPGASRRAAHIILNYLES"
     gene            complement(12972..13556)
                     /locus_tag="PLI55_01495"
     CDS             complement(12972..13556)
                     /locus_tag="PLI55_01495"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPA99544.1"
                     /translation="MDIRRLFFKIRGYTPIPLMLVLIYQSDMNFKLALIGAILALVGE
                     YIRLNGVRAAGGRTRTRNVGAKELCTWGIFAHTRNPLYIGNALIYIGMVFFAGGRWLL
                     PLMLLVFVYILFQYGLIISLEEETLTNIFGAEYAKYKANVPRLWPRLKPWNSGVQPKF
                     LSNRKLLRTERSTLSVFVIFALAVILKEWLNVRW"
     gene            complement(13556..14548)
                     /locus_tag="PLI55_01500"
     CDS             complement(13556..14548)
                     /locus_tag="PLI55_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPA99545.1"
                     /translation="MLKIGVVGAGNFGRWHIKNLQNLPNAELVGFFDNNPERASYISN
                     EFQVRAYPALEQLLDDCEAVSIVVPTHSHYSVAKAAIDCGRHLLCEKPFMETVAEAEE
                     IIRLARAKNLVLQVGHVERFNPALGALKQYQIDPLFIESHRIAPFRGRGTDVAVVLEL
                     MIHDIDLVLDLVTAPIVQVEASGAPILTDNIDIANARLCFANGCVANVTASRVSNKQM
                     RKLRIFQRDAYFSIDMLAGSTEIYSLSRDAGPVPDDTTQIMEVPDVNGAPRRIFYRVQ
                     QTEQGNALYNELESFVNAILTGQPPRVSGEDGIRALKVAIQIINSIQDNFRKIN"
     gene            14715..16193
                     /locus_tag="PLI55_01505"
     CDS             14715..16193
                     /locus_tag="PLI55_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPA99546.1"
                     /translation="MVINLWAELIAEVPQVVIVHWNDFHSTNLPYKSSQADYRVGGYA
                     NLAGYFDSLRHLHPQAIILNAGDDFQGSPVSNLTRGLSQILILNKVQPTAFTLGNHEF
                     DYGIDNLRNVMTYANFPILNSNLFDSTKNDLLVQPYTIIQSGNLKVAIIGALTGDLFT
                     LALPQNLKGIGILDPLTQIRQYVNEVEPQTDLIVLLSHCGFEEDSLLAMQLAGVDVII
                     GGHSHTYLHQPKKVNNILICQAGSNGHCLGYLKANIDRNQKIITSYNYELIETRLGRV
                     KPSPIVAGVVDSLEASISAEMDKIIGQLITDWHRNSHGESNLGNWICDVTREYFKTDI
                     AFMNSGGIRKDLKAGPIRVRDLWEISPFDNTIVIVALTGEQLKFIIQYHVENPRDLLQ
                     VSGLSYEFNSKQKKLVKLTVAGKPVDDQKIYTFATNNFVISQFERFFGLAPSAVKITP
                     TDIIGRDILIEAIKAQPVINNHTEGRIIDLANKKEEVNYDIQ"
     gene            16180..16611
                     /locus_tag="PLI55_01510"
     CDS             16180..16611
                     /locus_tag="PLI55_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99547.1"
                     /translation="MTSSSRILYSYIILGIALVAFGIATILGHFYIGLICLMLGMIIW
                     LIVTFAGNTEGQSGQIGVVMITAGFIGGLIIFIGIGLRPSIFGGHKLYIEGIVTALVV
                     LLIFMAIGLGFISLANLEKKMAFFNKELKKFYKLYMKEDNE"
     gene            16649..17287
                     /locus_tag="PLI55_01515"
     CDS             16649..17287
                     /locus_tag="PLI55_01515"
                     /inference="COORDINATES: protein motif:HMM:NF027727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HPA99548.1"
                     /translation="MALTQNLQLHYDFGRQHLTSTYELYKTDRLGATFLFVDFDYNNE
                     GSSGSRNASLAYGEIARYFQLPKMNDLSATLQYNDGLTNSGSFNSVWLGGLQYVFQIG
                     TQNFPIDFLLRKELNTDGLTFQLTYVWSYIWRKLELSGFIDIWNTGADAYPPRKIVIL
                     SEPQFWYRLTPALSVGGEIEISFNFSGAWHTNQVFSEDVIFILPTLGLKWLF"
     gene            complement(17416..17550)
                     /locus_tag="PLI55_01520"
     CDS             complement(17416..17550)
                     /locus_tag="PLI55_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99549.1"
                     /translation="MKYLKLIFKILAVITVIGLLILAVLLIIQKKSKSESVAEEPATE
                     "
     gene            complement(17566..18351)
                     /locus_tag="PLI55_01525"
     CDS             complement(17566..18351)
                     /locus_tag="PLI55_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99550.1"
                     /translation="MRKLFFSNATRVFFIVLGLCVLPIYLTPQSTKVKSILEQADKLH
                     DARDYQGTMTKLREAEKIEPNNPEVLWRLARAYFDIADQQPGVSEVQKANLYPGFEYA
                     KKCVELAPEVAGGHQYYAILIGQIGELEGTKQKITNSYAVREHTEKAIALDPKEDSNY
                     HVMGRWHFELADLSWAERQVASLIYATPPKASFEEAEQYFQKAHELKPKEIRHLLWLG
                     KTQLKLKKKDQAKKNLQLALEIPAQSDSDKVMQKEAADLLKKL"
     gene            complement(18433..19203)
                     /locus_tag="PLI55_01530"
     CDS             complement(18433..19203)
                     /locus_tag="PLI55_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99551.1"
                     /translation="MNRKQFFPFVFFMVLAGSLSAQGDLGIHQPVNLVTIPTAGILPR
                     GSYAVDCHLFPGGGLAGQISVGVFERFMFGVSYGGGGIIGNQKINWYNQPGVEIKYRF
                     IEESVKFPALLVGFSSQGHGNYIDSLKRYETKASGFYVVVSKNFNFLGNLGLHGGINY
                     NPIEKTDGDADPNFFLGIDKDLNSEIALIIEYDAALNDNHTELPLLGKGWGYLNAGVR
                     WRLVDKFTVELDFNNILLNRKDVDFMNRELKFTFTEIF"
     gene            19595..19771
                     /locus_tag="PLI55_01535"
     CDS             19595..19771
                     /locus_tag="PLI55_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99552.1"
                     /translation="MKGFGAVLVAISLILLVLEHLNLVDVVGIIGLLVGLYAIIFPDK
                     AEELYTKLKSGKLK"
     gene            19898..20113
                     /locus_tag="PLI55_01540"
     CDS             19898..20113
                     /locus_tag="PLI55_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPA99553.1"
                     /translation="MPIYEFECQNCGRVFEELVFSKNFSESEITCPHCGFKQAKKLIS
                     APALSKSSACSSCGAKSCPSSCGSRSH"
     gene            complement(20275..21099)
                     /gene="proC"
                     /locus_tag="PLI55_01545"
     CDS             complement(20275..21099)
                     /gene="proC"
                     /locus_tag="PLI55_01545"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPA99554.1"
                     /translation="MKNNQIAIIGAGNIGTAIANGLADSNRFKPAEIILTRRRIHLLD
                     DMQKRGFIIEKDNCQAVRQAKIIIIAVEPQQVNSVLIEIAPHLKPNHHIVISVVTGVN
                     TSQIVKKVGSGIAVVRAMPNTAIAVRESMTCLASDTASEAEIAITKSIFDTVGKTIVI
                     EEEKMTAATALGACGIAFFLRAIRAASQGGIEIGFHSQEAIDIAAQTARGAATLILTH
                     ANHPEYEIDRVTTPRGCTIAGLNQMEHEGFSSAMIKGITVSAEHAKSLYKNTNNSD"
     assembly_gap    21185..21284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(21568..22962)
                     /locus_tag="PLI55_01550"
     CDS             complement(21568..22962)
                     /locus_tag="PLI55_01550"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA99555.1"
                     /translation="MTGNHARKIEYLKKKASRISIREGAAYGLFDGFGLRYITPYALL
                     LGMSNTLIGILISLPQMLGTLIQLLTLKFLEKGIHRRTIAWVGTSLQTLMWFPIIFLG
                     WLHFNYNLNNMVISAGLMVFYSLMVIFGGIVGPAWSSWMGDIIPRQIGTYFGLRNRVV
                     GFTTILAMLSAGLILQHFNRLGQPFTGFAIIFLIAFTGRALSSALLKRQYEPEFHPQK
                     EYYFTIWQFIRKIPQSNFGKFVTYVALMQFAVYISSPFFAVFILEDLHFNYFQFTLIT
                     MTMPFVNMLLMPAWGRFADRFGNLRVVRLTGFCLPIIPALWVLAVFFIDRPVLAVLYL
                     ILVETLSGFAWAGFNLTTVNFIYDAVTRQRMAICVAYYNIFNGIGMFLGGLIGGLIAD
                     LPFQFHRFSALALVLMLSALIRLLVSAVMLPLIREVREVDHFDIKEARKRLAMLSPVD
                     ILRVLSLKLGNPRH"
     gene            23114..23189
                     /locus_tag="PLI55_01555"
     tRNA            23114..23189
                     /locus_tag="PLI55_01555"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23147..23149,aa:Lys,seq:ttt)
     gene            complement(23662..24306)
                     /locus_tag="PLI55_01560"
     CDS             complement(23662..24306)
                     /locus_tag="PLI55_01560"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPA99556.1"
                     /translation="MAIKAVIFDFDGVVINSEPLYMVAERRLFDQYGIKVPDEDWKYF
                     KGTTEGDFYQLIMAKYQINTDVHKLIEDGRRYIKEEFNKGVDYLPGFLDFWQSIDGHY
                     RTGLVTSTSASFLEWVFRNTPVRNIFDLIITADDVYQAKPHPEPYLKAFESLRVRPEE
                     CVIIEDSMTGIQAAIASGAVTIGFRCDRNDRDMPKLHFYADNYTEIANILSKIG"
     gene            complement(24457..26076)
                     /locus_tag="PLI55_01565"
     CDS             complement(24457..26076)
                     /locus_tag="PLI55_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPA99557.1"
                     /translation="MISKKDLIIKIALRFIDVPRVEIDSRINESLRDLGEFYQTEYCL
                     VSALLPDTKEALKIYGLEDKSEDSVKLLQNIALANSPWLKNQLSLKGTVIINDVNNLP
                     AKANREKQELQQLEIKSLMIAPLVYNDTNFGLLIVAVSKTSRRWLDSEIELLKIVASI
                     ITSALHRKKTEEKMAETMSLYHLFIDKINEGIAICHKERFIFVNNKLAEMLGYTVDEL
                     IGMEFHNILTNRGVEILKERERRQAIGKTVPKRYETSFKCRDGHTIEVEVSPVEIEYR
                     GKLTTFAVISDISEYKRLSLAKQALEVKWLKQQRLMSLTLMIGGIIHNIKNTLTVVMG
                     RAQMLKTRLPELKEPDIIVDNVRKIESLLTSFIDKMNLELENKKITINLSELLRNELL
                     FLETESFFKTQIEKEIVLRRDLPLITGHYSDFSQSLMNIIHCCVESMRDSPRKILKIE
                     TGISQDNIYIEIATTGQPIIPDNAEELFTPSFYHEIPENENASDQKALIKYNLYNAYM
                     LLSSYKAKFSAFNNPESGSTIRVELPIESNN"
     gene            complement(26179..26799)
                     /locus_tag="PLI55_01570"
     CDS             complement(26179..26799)
                     /locus_tag="PLI55_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPA99558.1"
                     /translation="MSRTNNLIKAVFFDLGNVLVKVNLESAIPQIAELFRIPEKAVMQ
                     APILALEKKFERGQLTIAEYLVEVKNIYHIDHSIGIDDLERIWQTTFELNTDVWQIAL
                     RLRRQVPLFLLSNTNALHIRAIRRKFNILENMDGLILSFEVGATKPESAIYQYALRKA
                     DVTAEQALFIDDLKENVAGAERLGLKAHQFISASDLENILSQYSFK"
     gene            complement(26834..27817)
                     /locus_tag="PLI55_01575"
     CDS             complement(26834..27817)
                     /locus_tag="PLI55_01575"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPA99559.1"
                     /translation="MLLVSKLVRGNAVDSFHIGYAVVVDENGEILFSAGDPEYPIFIG
                     AAANPFQAVAMLESGALEQFNFNDEELALMCGTHTGEHYHLDVLNSILKKMDLSIDNL
                     LCGVHPPLDRQTYEQLAIQGRRPTALHNANSGAHAGMLAIAKALKYDLSNYTEINHPA
                     QQKIFEKIKLYSGKERIPVATDNSNAPTYLMPIYNLAIMYKKLVEGSDDYLRRVFHSI
                     SLHPKLIGGRAQFDTDFGALMGGRGISKFGMEGVRGIGLRNNENNPIGIAIKVLSGNR
                     EACDSMAVAVLKHKHLIDDEIAKKLDSYLSSAVKSYTDVEIGKFFTEVVAE"
     gene            complement(27833..28531)
                     /locus_tag="PLI55_01580"
     CDS             complement(27833..28531)
                     /locus_tag="PLI55_01580"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HPA99560.1"
                     /translation="MHYLEINLDPFIELGRIVDIPLGNPLNLLGFIEAVGVAGISFTN
                     TPERKGMINITALRSMSRCRLNIRANIENQAIQRAMSAKPDSITLINLDESDLTVDPH
                     SQIVHNLIETVQSAEDFNLILRLKPEIRGLKAAYRLKVDEVELATDPLVTQVLQVSYT
                     DELNKIVAASRLAIRNHMRVSLGGRLHKRLILSLLELIDVEFISVGRALLGQALITGF
                     DSALRELLEIVENR"
     gene            complement(28536..29696)
                     /locus_tag="PLI55_01585"
     CDS             complement(28536..29696)
                     /locus_tag="PLI55_01585"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPA99561.1"
                     /translation="MTRTSNYLKKPSKIVEKRHRDEKRLNALFSTVIGMQIILLVYFL
                     PKMAPSRANLFSEGKSRLINVARPFLLERGCQEIKSSSVATDRVSLIFELPASRDCEL
                     LAQQVSAYLNEHGFMVKKIDNLVKGTGFTLFVDFQHIPIGTLAFIKTDRPYPARMPIS
                     GVKPKLAIIIDDFGYTNQEIVSNFLKLPTKLTISVIPGHPYSQWVARNAKLAGKEVII
                     HMPMEPEQSSNSYGEDKYMLLTGMTPEEISHRVEMACAELPEAVGMNNHMGSLFTSDP
                     ELMQIVINSLKRKGLYFIDSMTSAESVAYEVAIRNQIPTALRTVFLDNVRDKGEIQAQ
                     FERAIEIASRGGHAVAIGHICSETLSALTEILESGLLNEVELTFASEVTSLK"
     gene            complement(29805..31160)
                     /locus_tag="PLI55_01590"
     CDS             complement(29805..31160)
                     /locus_tag="PLI55_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA99562.1"
                     /translation="MDKIGTDVELKPPSARYRWLVFFFISMAMFGNYYIYDSISPLAD
                     LLKAQLGYSDANIGLLNAIYSFPNIIMVLIGGLIIDRIGTRKSVLIFTILVMLGAVIT
                     ALSGNLLLMATGRLIFGLGAESMIVAITTILARWFKGKELSFAFGLNLTFARLGSFLA
                     LNSPTWGRSLYTSWQKPLLVTAVAGMMALFFIGIYCLLDLLASRRLLLPQESKQDKIE
                     LREVFRFGAGFWYITALCVTFYSAMFPFQTFAIKYFQEAHGTSREVGGNLSSMLTLAA
                     MIFTPLFGLVADRTRRKAWLMALGSLLIIPVYLIMAYHIDLATPLGFHDIINIKFSFF
                     GINSSIPAYLIIPMAMMGIAFSLVPAVMWPMVAIIVDNNRLGTAYGLMTMIQNIGLFS
                     FNIIIGLANDFTGASADNPGGYNLGMWIFSILGFCGLLFAFRLGKYEKSMHHEQIKEN
                     G"
     gene            31331..31498
                     /locus_tag="PLI55_01595"
     CDS             31331..31498
                     /locus_tag="PLI55_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPA99563.1"
                     /translation="MAYVISEDCTACGTCIDECPAEAISEGDIYKIDPDLCTDCGACA
                     EVCPSEAIHPA"
     gene            31826..32512
                     /locus_tag="PLI55_01600"
     CDS             31826..32512
                     /locus_tag="PLI55_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99564.1"
                     /translation="MSSRRWRAGAGLTEIQRHIHWVADLPDIITVILSDGSLNELETL
                     FDNHKIILAPNNGTEIYAADFSWNLPNLNLIRQELTDIYCAVRESLGAKLQPEYLGFS
                     QSELWLKVSPGAEAVRKSLIEQFKLQNPAHLRLITQNNQTTIVPLIKWDRGQVIQKLM
                     DLIPHPLNQTPLVIYFGVEENDEAAFHYVNQVGFSVIIHNRIPRATEACYYLRNVAEL
                     SKFLFWVHSR"
     gene            complement(32577..32828)
                     /gene="rpmA"
                     /locus_tag="PLI55_01605"
     CDS             complement(32577..32828)
                     /gene="rpmA"
                     /locus_tag="PLI55_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPA99565.1"
                     /translation="MAHKKGVGSSRNGRDSRGQRLGVKRFGGEQVSAGTIIIRQHGTK
                     IHPGENVGIGSDDTLFALIDGKVKFSRKGRDKKIVSVLA"
     gene            complement(32834..33247)
                     /gene="rplU"
                     /locus_tag="PLI55_01610"
     CDS             complement(32834..33247)
                     /gene="rplU"
                     /locus_tag="PLI55_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPA99566.1"
                     /translation="MNNQNYAIAEIAGDQVILEPGKNVAVPKMDLEVGAKFLANKILF
                     IHTGNDIKVGKPYLEDFAIETTVLEHKRNSKVVIFKKKRRKGYRVKKGHRQPYTVLQV
                     SEFAKPEAAAPVKKSRAKTKTVSESAEPAEQEKKE"
     gene            complement(33440..>35190)
                     /locus_tag="PLI55_01615"
     CDS             complement(33440..>35190)
                     /locus_tag="PLI55_01615"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPA99567.1"
                     /translation="LLDDQSPTVRTGAAFALGQLASQTAESLLVQHLLSETDTEVRQQ
                     TVLALAQFGNRSTLNFLLETNSEYLGQALIYYFQRQIYNPASLNYAVSHLQSNRHSEA
                     YQSALALSRVQNPTVMQEYVDILKTALKTAGQATRVKIVTCLAPVNFSGKAEIWTQLL
                     TDSVPEVRLEAARNIRIVTHDPEILHQVFNDSFPQVVATAIENLPDSLELIPVLQDDI
                     RTLAHHPSRAVRSALVKFLSRRGGQETLANFGLWPVETNLLPAFAEGIAASGQISGLA
                     VLDSLSRHQFRAISTPAYLGLINLAEQFCRQDSIPCEQFCNYIAAGLKSDDPVKIAVA
                     AESMRDSTTKCENLVPLLYPPLKKFKNAEYADAVIEILKTIAVRQPPDAVPFIQPLLN
                     ARDFRIRDLAYQILQEIYEANVPEIINDYRGAGRPSNLKMLEKYGLRPTILLETECGN
                     IIIRLDGYYAPFTTDAFLSAIERGFYNGLNFHRVVPNFVVQGGDPRGDGWGGPGYTLL
                     TERSPIEFNCGAVGMVRTEYDTEGSQFFITLTDQPQLNYQYTRFGEVICGMDVVKRLE
                     AGDRILAISVVTMDER"
BASE COUNT        10301 a         7363 c         7268 g        10058 t
ORIGIN      
        1 tttaaattat cctcattcat aaagaaatcg gcatattttc cgtaattaac atcgttgact
       61 ttgtgagcta taagcatggt caattgggca acacgttcga tatgaccgcc ggtgtagggt
      121 gatttttcgt caatagcaga ggcaatgact tggatgaaag attcaaataa attttcaagg
      181 tcatggatta ggcgggcatt agtaatggct atcgcggctt gtgaagccag cgaaattatc
      241 agcttctgat cggtagatga gaaggggcaa acttcgccag tgcggcggtc ttgagcattg
      301 aggatttgca gaacgccgat gatttcatct tcgtagttgc gtaaagggac aaccagcatt
      361 gacttggagc ggtacccggt attcttatca aaggcgcgtg tcccggtaaa gtcaaaaccc
      421 ttaacgttgt aaacgtccgg gatattgata atttctccgg ttagtgcaac atacgccgaa
      481 accatagcgt ggttgggttt accatccggc agatatagag gaactggctt ccagttgatc
      541 gggttgccgg aagtgccgcc catcttgatg ttaagcgagc cggtctgcac aatcgcgaaa
      601 ttgagccatt tgcgatcatc actcatcaga taaagcgtgc caccgtcggc attggtaaag
      661 cggcgcgcct cttccagaat catctggagc agaaccggga gattatgctc ggcggatagt
      721 gccgcaccga ttttggacag attatcaatt ttatcacaca aatcggcgat aaaatgctga
      781 accgctggcg aggcattgct aatctcagtc gccaaagtct gatcatcagt taatttatcg
      841 gagaattgat aacctatgtc aattccagag gtgttttcca ttttataccc tcatactcat
      901 tgtgttttca taaagttcaa ttcgacattg ttgatttaaa atttacattc tgtttttcga
      961 taattagcca cgaactcaaa atcaatccca gcaggattaa caaaatagcc gccagacgac
     1021 cttgaggtga acctgccctt agcaggctga aaatttgatt aatcataaaa taaccaaccg
     1081 ccgcgtagag atatgccggt ggattggcac cgacgaaata cttcaggcaa aaatatagcc
     1141 ataggatcgg caaaatgaat tgtaggtaaa ccacaagata accggaaagt gtcaggtaat
     1201 tatctggtag aaaaacagca gtcagcataa taagagtcat tactttccgg aagttctggc
     1261 cttttaggtt ttgtaatatg aacccgataa aggcgatggt ggcaaccgac aggagtgaca
     1321 tgattattat tctctgtaaa gcaccgaaaa atgggaagag cgaatcttcc agaccggatg
     1381 taatttgatg attggcgaga agctcaggct ttatcattga gataatgtag tttgataagc
     1441 gattacaggc taaaagtcca caaatgatca aaatggaagc aatcagagcg tcgcgcgtgt
     1501 atcgcggccg atgcgctttc tgtaaagttt gaatagcgtc cggatataaa agcccaaccg
     1561 ttgaaaaaat gaggacggtc aataagctga ctccaatcgc tttcagcaaa attgataaaa
     1621 tccacagaaa attccagtta gtcagcgtcc agctggtgtc gtaatttagc aaagccgccg
     1681 agtaatttaa cccactgacg ataattgcca atccggcaag atagagcgcc aaccgaatag
     1741 cagtccgcca gccgctgaaa acctttttag gtctatttaa taggatcttg ataattctat
     1801 aaagtattag gataatcaga gcaatttgca caccggtcat tatggattga aaagtcgttt
     1861 ggcgcgtttg agccaaaagc caatcctcgg gaattttata atcggtggcg aaagctgcaa
     1921 tttcgtcacc tttgagggtt accgtgagtc gtaaacgggc atcggcaaca ctcgccggat
     1981 gctcatcccg actctcccag ataaaatgat aatcggtacg gttgggcaaa acatccgaat
     2041 tcgattcaat tagtgaaaaa ttactcagtt catatttttc gaaggttaaa aattcctcgg
     2101 ctttgcgctg ggcgctggct tttgacaatc tcgccccggg ggcggtctca tccaagatat
     2161 ggctaaatgc tgccaccgta tttttcattg ggtgaacgta aatgcgatat tcctctcgtt
     2221 ggttgggctg gaaaaaacgc acttgccagg taattatatc gggtaaaaat tgagccatta
     2281 tgaaattgag ctggttcaag gaagaatgct ggattacata ctgacccatc aatggattat
     2341 aattattatt cagccccatg gtgattttga aatcactcga cacaatctct ttttccgcca
     2401 gaaattcctg agcagtttgt ctaatttcct ttttcggtat cggaaagcga taaaaatcac
     2461 caactcgatg aacgggtatt aatagaatta gtaaaaataa agcgcttatt attaaaccgt
     2521 tgcggatttt acgcggagag tagctctggt actctttcac ttccgaaatt tcggggactt
     2581 cattagtaat ggttttttct ggcttgataa taaacactgt ttcaccgtta agcaagccgg
     2641 acgaatcagc gaaactacga ttgcgccggt agcaaacaat gttataaatg ataggtagca
     2701 ctatcacgcc cgccgctaaa atagcggtta cgatcagata cggctggcca gttttaacta
     2761 aaaatacagc ggtgtaaatg gcatcaaccg tataatgcca aactagaacg gtcaaaattc
     2821 catatcgcca aaataaaaca cccacaaaaa tgccaaaaag gctaacctcg gctcctcgaa
     2881 tccagaatgg ttggttggga taattggcat gagcaaatcc ccaaatgagc gccgggatta
     2941 ggatcgccag cggcttagag cgaaatattt tcgcgaaaaa cggtatagca aaaagacgaa
     3001 aggaaaattc ctccgttacg gcgggaataa atccgatcag taggacaaaa atccatggaa
     3061 aataggtatt aatagcattg gaataattca cctcgactgg cgcccaagcc ccgaaacgat
     3121 tggcaatgag atagaaagcc gtttgaaaag caatgaaaac aaccgccagg atcatcccca
     3181 gagttgaatt gattaaaaat gatttggtgc gtaaaccggt tggactaaat agcttactca
     3241 tagaaagatg ttggggataa gcacgacgat aaagcacttc accggctcca gtgattactg
     3301 ccaccaggag ggcataaagc agggcgatta caaaagtctg aatgaaataa tttccataat
     3361 aattcccgag tgactggttg gtatcgaagg cataaattgt aatcggcaat tcgttgatgt
     3421 taccagcaaa ttgtagtata aaagtaatca agccaaaccc gagcgctgtc ttgaggtgaa
     3481 cattttttcg gttaagttga tttataaaaa caaccaatgc cgctatcaga attaatacga
     3541 ataaaacatc ggcggctaaa gccgtagtcg agttcagcga acgcaggtgt ttataattgc
     3601 tcaaccattg ttcgggtaac ttccagtatt cacgatattt gccgaccttg cctcccacta
     3661 tgccgatctc aagccggtaa gtcgcactgg aaatttcaac ccccttcttt tggtaagtaa
     3721 aaaaatgatc gacgcgattg ggcttgatgt cggttttatc ctcaatgaac tcccactcgg
     3781 ccgggttcat ttgcatggtt gtcattagaa aatcctggca aatccggcgt gccgcttcaa
     3841 ctgaaatgcg ctctgccgcc gcttcttccg gcaaaaaatg ctcaaagtaa gttatcctgc
     3901 cgttcaatgc gagattgacc ttgatttctt ccggtgaaag cggcttaaac cagcgatttg
     3961 accagtgcca gatggggaag tcctgacaga gcaagcgcgt ggcttcttca aaaccaactt
     4021 ctttttcgat aaaagtcttt ggcaattcat cataatcgaa aaccaccgca tagcgatagt
     4081 cgttcaaatc aatatgttgg tgactaagga atttttcagc taaatcacgc gaatcagccc
     4141 gtgttatctc aaacttcagg tcataagccg gaaaagcttt gcggtaattg cggctgagat
     4201 aaataattga aatcaggagc aatgccccac atatcgccag caaattccag tctttgtgcg
     4261 tcagtttatt gtcgttcata gtgttctaaa aaacttgctt taaaataatt ccattttata
     4321 taaaacaaaa gactccgttg aaacggagtc ttttnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnactttt tctgccatag taatttcctc ctaaagtttg tttacaacat
     4501 ttatgggtaa atgttaaggg acaaaaagta aaatgtcaag aaaatcttcc atctaaatgc
     4561 atttttttcg gcatttaatg taaaaaaagt ggaaaaattg gcttcattga ctggtgatta
     4621 tgactttttc agtctctaaa gcaatcacca actcctcatt agtcgggact gccaggacat
     4681 taatttttga ttctgcactg ctgataatac cttcgacgcc atgggcaatc ttattatgtg
     4741 ccggatcaag caagatgccc agattttcaa ggttagatag agatttttct cgcacaaggg
     4801 cagaattttc acctatgccg ccggtgaaaa caatcaggtc ggccccatta aggactccga
     4861 tataagcgga gatatacttt ttgacgcggt aacaaaaaat atccagcgcc tggcgagcgc
     4921 gttgatgccc ggcggccgcc gcattttcta tttcacgcat atcactggaa agcccggaaa
     4981 gtcctttcat accacactgt ttattgaaaa aagtattagc ctcttctata gaaaaatttt
     5041 cacgatcgat aatagtcaag atcaatccag gatctatatc accacaacgt gtacccatta
     5101 ctaaaccttc cagcggagta aagcccatcg aagtatcaac cgaaataccg cctctgaccg
     5161 ccgcaatgct acaaccgttg cccaggtggc aggtgattat atttaattgt tcaataggtt
     5221 ttccaatgat ttcagccgct cggttagcaa cgtaataatg cgatgttccg tgaaatccat
     5281 agcgtcgcat cccatatttt tcattgtagc tataaggaat ggcgtacaga taagcatagt
     5341 ctggcatatt gatatggaag gtagtatcaa agactcctac attaggagtg tgcggcagtt
     5401 ttttcaaact tgcctcgata cctttaatgt tgtgtggatt atgcagaggg gcgaattcga
     5461 tacattcgta caacttttca cgcacagcct cattaattag aactgactgc ttaaaactag
     5521 ccccggcgtg cactaaacga tgaccaactg cttcaatttc tttaagactc tgggctacgc
     5581 cgtgggtagg atctattaaa atgtccataa tagaattaat cgccgtttga tgatcaacaa
     5641 tgttatcagt gataacgact tggtgaccat cctggcgttt atgagtaaga ctagtgccgc
     5701 ttatgccaat tcggtcaact aatcctttgc aaagtacggt tcgagtgctg gtatcaataa
     5761 attgatattt gacagacgaa ctacctgaat taagcgtcaa tactttcacg ttatattatt
     5821 tccactattt tgagttaaaa attgttggta gaatgagggt aaaattctta atagtgccac
     5881 atcagcttcg caatttatgg caaatattag taaatcttcg tctattttca aactattttt
     5941 agagagtgag gactggttta tgaccaaaga cacaagtgat taaaaagtat gattgtaata
     6001 gccaaaatat tttaaaagag cgcgaatatt tttttaaggg ttattatggg tgaaaccatt
     6061 tgatatctga ataacagaag catctcaaaa agtgtggcaa aagacaaaaa aatggctcac
     6121 cttcctgtct gacctgtcta cccgacaggt ggatagatgt tgtttaaaaa cctagaaaag
     6181 gaaaggaaag ccatgaaaga gattaatttc aagattgatt tagcgaatga gtatcacaat
     6241 ttcaatcagt ttgaggcgaa attgattaag ggaattcggg agaaggtatg tgagcaaata
     6301 gtacaatttt tagaagagtg ggatcaggag ttagggacac gtttgagaca gcattatcct
     6361 caatatcgtc ataagggcta tattcatcgt caggtacgtt gttattttgg ggtaattggg
     6421 attaagatga gacgttatgt tcataaaggt tggccggata tatatccatt attgtttatt
     6481 ttaccggagg atggaattag tggagagcta atggacttga tgttggagtt ggtcacagat
     6541 gtaccttacc agaggactaa tcgctatatc aagaaagcca caggggtagc gatcagttcg
     6601 caaacgatct ggcgtaaagt aaagcgctta ggtcaggatg aacgtgatcg acaggagcaa
     6661 atacggtgca agatatttga agagggtggt gatgattatt gtcaggatta tcgtcagtta
     6721 cctgcctgcc agtcaggcgg caaagagtca gatttagtta gtccggtcta catagaactg
     6781 gatgggacga tggttagtag tcgtgaggca ggagaagaac gttttgagat taagagcggc
     6841 ataatgtata gccaggtagt tcagatgggg gagaagcgtc gtcgtttaat ggataaagta
     6901 gcttatggta gtgttagtga tagtgagacc tttggtgaga atttttatgc cttttgtcgt
     6961 gagaatggac tggatttacg ccgggagata ttgtttctga gtgatggtgc tcaatggtta
     7021 aatagtgttg ctgaggatgt gtttccggaa gctcaaaagc ggctggattt ataccatcta
     7081 aagaaagcct gtggtattgt tttagatgag caggaattaa atactttgag ccaggcagtt
     7141 tattataaaa ctggcgagga aatcattagg ttagttcaga atttatgtag tcggaagaat
     7201 ctatctggga agtcagtaga tgaactgatg caatatttag ggaataatca aaaagccatt
     7261 gaatatccac caggggagcg tcatggctct ggtggaattg aaaagaatat tggtattttg
     7321 gttggacgac gctttaaaag acaaggtatg tccctgactg ccattaggca agtgtggtct
     7381 cacgaaggcg ctaacaatct tctcgcccta agagcaaaaa aattaaatca gatatggcaa
     7441 caagaatcac aagctttaaa ttataccagg tgagcgccac actatttgtt atgcttccct
     7501 gaataacttt tagcttgatt ttttctctat tggcaaatat attcaaatgg tgataaaata
     7561 ttttatttaa aatatatggg aaggaaagat gaaatttaaa attacgctcg tatttataat
     7621 atgtttagtg ctgtcaggaa tgatgaattt cggtttagcc cagtccgatc aaccgctaat
     7681 tttttctttt aaacaaaatc atacggttct ttataaaata gctaaatgct caaactgcga
     7741 taatgatgaa aactttgatg taattggaat tgcaggcttg gtagatagcc atggccaatt
     7801 agttccgcgc tctacttatc tggttcatct ggaagaatcg gaaaatagcg attttacagt
     7861 ccagtggagc tataacttgc cgcaggatat taaaggtgat ttcaccgaca tagccgtaat
     7921 taattggaac aatgtaccag ctattattgc ggtattaaat attaccgaag ccggcacggg
     7981 tcctgatcca ggttggttat tacttttcga atattcggat ggattcagta aagaacctac
     8041 agttcggatg accaccgata ctgtcttccc tatcagacct cgtccaagat atctcagtgt
     8101 cggcgatctc gatcatgatg agcagtctga cttaattatc tcatcctcca gccccaatcg
     8161 gagtattaat atagttactc aagccgccga tactagtgct ctttctctta agttaattaa
     8221 ttattccagt gatctgcctg ttttactcgg tcttcaacct tttcgggcat tggcggctaa
     8281 tgttgatact gaaccaggtg atgaaatcgt cattttcggg ggacagcaga aattaacagt
     8341 tgaggtttat cgctcagatt tgaagaatcc attatcttca tatacttttt ccaatgtgaa
     8401 tcgtaatgac attaatctcg accaacttgc ggccggggat ctggacggtg acggtttggc
     8461 tgagttcatt ctacctttac gtagcggggg agctcagctt ttagtaaaag gagtgcaaag
     8521 ctacaaatca ggtctattaa taccaaaaaa gaacaaaata gcggctctta atttggttga
     8581 tattaacggc aatggtctcg atgaaatttt atgggctgat gccgccggtg ccgatattta
     8641 tcgctatgaa tataatccag gagcgaattt attcgatcta gatacctata tttcttttca
     8701 ctactcaaat ccggttttag ctaatgtgca gttattatcc attgcgccgg tggtttctta
     8761 tggaggcaag aatacagggg caataattac cccgtttctt catcgcagtt ttaaccaaca
     8821 tggattgtgt tattggcttc tggaagagac aagtccgctg attgatgaga gcttaattga
     8881 aacaattctg ggatcggttg actcagtact ggctcataag gaatttgatc agaagaaggg
     8941 tgcatcttcg ttgactgaaa tggatttatt aaagcaacaa ttgggtagcc ttgttggtac
     9001 agaaattgaa ctttctccct taccggaagg cgaaggtaaa ccatcaggta tttcaggcga
     9061 ccggcaaatt tatcagcctg atatactatt gcatcccggc gagttaatgc gttataattt
     9121 aaatcttcca gatttaagcc tcgatgatac taaaaaatta agtgtaaata tcgatgcgcc
     9181 atcaggtatg aaattcgacc tagccaataa attgttcact tgggttccag ccgattccca
     9241 attagggctt cataaggtta ccgccttgat ttcatgggat gagaaaaaag atgtgcagac
     9301 ctttacggta tatgtaaaca gtatccccaa aatattaacg aagatcccta tccgtgatat
     9361 tatccagatt ggtgaaacat tcagtctgca aattgaggtg gaggatgcca accaagacgc
     9421 ggccattttt tataaattgc ttgagtttcc cgccggagcc agtatcaatc aaaagggaga
     9481 attattgtgg aaaccctctt tcgatcaagc tgattggtat gattttatag ttgaaattag
     9541 cgacggttac gattcagaac aaaaatcttt tgccttattt gttaatcacc cggttagcat
     9601 ccaatctacc gcccctaaga caacaactat tgggaaaaaa tatacctatc agccggtcat
     9661 tagcgataat aataagggat tctatttaac cggttattcg cttccacccc ggatagagaa
     9721 ctggagtcaa actggtgtcc tcgaaactca gattcttgac aaaaatattc gcaataatat
     9781 tagcaaatat attgaaaatt acagaaaatc ttttccatta tttatcacac cggcaaaaat
     9841 gaacgatgtc ccccagagtc ttttccagga tatttttatt gatagcgaaa aggttgtgtt
     9901 catttataat cttcgaaccg ctaaaggtat ggaagcaggg caaatcattg aaacgttttt
     9961 cagtaatttg aagatgagca ttccaaaata ttctactccg gttcgtcgtt atctctatat
    10021 ttttaatctt aaagaaccga tcgccgggtt gacgatgaca ggcgagggat taatcacctg
    10081 ggttccagcg gctaatcaat ttgattatca attaatttcc tatacggtga ccgacggctt
    10141 tttctcagca gaggagcagg ctcaggttta cgtaaattac ccgccgaaaa ttacttccaa
    10201 gcctgttact attgcctctg ttaataccct ctggcagtac gaagtcaagg ttacagacct
    10261 aaatacagat agcaaactaa cttatgaatt gctcagcgct cccgaaggta tggtaatttc
    10321 tcctcaggga ataatttcct ggcaaccaac cgatttacaa attaattctt ataactttac
    10381 gatcaaggtc agtgacggat tcgcccaggt tgttcaaaaa gtacacctat tcgtcaatgt
    10441 caagccgaaa atcacctctg tgcctaagcc ggtagcgcta actaatttaa aatatgaata
    10501 taagcttgag gcggaggacg ctaataatga taagctgact tataaggcag tccgattgcc
    10561 taaatatgcc agttttgatc cgaccaccgg tatgcttgtc tggacacccc gcaaagacca
    10621 aataggggct aacgatatcg tgctgaaagt cacagattca catggcggaa gtactctcca
    10681 tgaatttcag gtccatgtct ttgctaatcc ggcgcgacga cattcttttc tacgacggac
    10741 gatatcgctc ttgactattg ccggtgtgat ttacttagtc gtaatttatc tataaatccg
    10801 ctccggaaat agatgaaggc ggtaatcttt gcaagatagc cgtttttatt tcatcagata
    10861 gttttagttc aatttcgata accaaaagcg gaatagccaa tgatccagca tttaccggca
    10921 ggttgacagc gtctttttca gtcgtgacca gatagtcaca gttcatctgc tcggctttga
    10981 ttattatctt ctgcaaatcg gtggtcgtat aatgatggtg atcgggaaat cgtaggaatg
    11041 tcaccttacc acagtttaat tgtcggacgg ttgtctcgaa ctgaatatgg ttgccgagcc
    11101 cacaaaacgc cagtacattt ttaccagcga tagtattgat tgagacgttt tctgcggaaa
    11161 gggtgcgaag accaacgatt tttagagaag ccgtaaaaaa tgttatgctc ggattaaatt
    11221 tctcgaagaa ggttcgttgt tcggggatga ttttatccgt cttagaaaaa actaaatagt
    11281 cggcgcgttt taatccgtgc caggattctc taagccgacc gaccggaaaa aggtactttt
    11341 gccaggacgg tgttttcgga tcccaaagta cgatattaat gtcacgggcg agtcgccgat
    11401 gctggaatgc atcatcggca ataatcaaat tgcaagcaaa attttcgatt gcggtatggg
    11461 cggcggctat acgatcagca tcggcaacta tgacaactcc gggcaattta ccagccatca
    11521 aaaaaggttc atcgccagcc tgttgtggag tagttaaaat ttgtctgcca tcggaaacaa
    11581 taactgtacc cctggattta cgtcgatacc ctcgagagat gatggcaatt cggtaacctt
    11641 tttcacttag aaattttgcc agcgcaatta caaacggtgt tttgccagtt ccaccagcgg
    11701 taatattgcc cacggcgacc actggaacgg gcactttgat agctttaaag atatgacggt
    11761 cgtaaaacag attccgtaat tttgtgatca aaccgtatat aacagccgct ggaaataaaa
    11821 gccatcttaa ggcacctaat tgcattttct attaatgtat ttatgattct aaataattca
    11881 ggataatgtg agccgcacgt cgggaagcac ctggttcgcc taacaggcgg cagacatatt
    11941 gcaaaaaatc gcggatttgg ttcgcatatg attgatcgca cagaattctc atgatttcac
    12001 ttttgatgtt ttctacatta gcatcgtgct gaatcaattc cgttacaccc tttttcccag
    12061 cgatcaaatt ggtaatcgaa ataaagggca ccttgattag ttttttcccc agccagtaag
    12121 tcagcggtgc cacgcggtaa attacgacga aaggcttgtt actaagcgtg gcttcgaggg
    12181 taactgtacc ggaagccata attagtacgt cggcagattg aatcacagaa cgggtactat
    12241 tgaaatgcca gcttagccaa tcgaactgat agcatttttg gtatttttcc agattgacgt
    12301 cggaagcaac cgcaacagcc gcctgtaaat ttggaaattc ctggcgcaga tctttgaccg
    12361 atgaaatcat tagcggtaga tgcttatcaa cttcttgctg acgactgccc ggaaacaatc
    12421 cgaccagcgg ctcgccgttt ttaaaaattt tctggtttgg ttccggtata gaaatttcat
    12481 tctggtctaa taaaggatgt cccacaaatg ttgcccgcac tcctcgttcg ctaaaccagg
    12541 tggcttcaaa tggtaaaata cagataactt cctgaacgtg gcggcgaatc ttcttaaccc
    12601 gggattgatg ccaagcccaa acttgaggta cgatataata aatcactggt atatcacgcc
    12661 ggtcgagcgc cttagccaag cgtaagttaa aaccgggata gtcaattaaa acgacgacat
    12721 cgggtttaaa ttcatcaaga aactgcaacg tcttacgata catactgatg ataaatggta
    12781 gatgtttaat aacctcaaca aaacccataa aagacgtttc gcgaatatgg aaaagcagtt
    12841 cagcgccagc cgccgccata ttatctccac cgatggcgct gatttcgata tcaggattta
    12901 attctttcag ggcggaaatt agtttgccag catgcatatc gccagatagt tcaccggcag
    12961 agataaaaag tttaccagcg gacatttagc cactctttga ggattacagc taaagcaaaa
    13021 attacaaaaa ctgacagtgt cgatctttcg gtacgtagta atttccgatt gctcagaaat
    13081 tttggctgaa ctccactatt ccagggtttt agacgcggcc ataagcgcgg gacattggct
    13141 ttatattttg catattccgc accgaaaata ttagtcagcg tttcttcttc gagagatata
    13201 attaagccat actgaaataa aatgtaaacg aacactaata acatcaaagg cagaagccag
    13261 cgcccacccg cgaaaaacac catcccgata taaatcaaag cattaccgat ataaagcgga
    13321 ttgcgtgtat gggcaaaaat gccccaggtg caaagttctt tggcgcccac gttgcgagtg
    13381 cgggtgcgac cgccagccgc ccgtacccca ttcagtcgga tatattcacc gaccaaagcc
    13441 aaaattgctc cgattagagc taatttaaag ttcatatcgg actggtaaat cagaactaac
    13501 ataagtggaa taggagtgta accgcgtatt ttgaaaaaaa gacgacgaat atccattaat
    13561 tgattttcct aaaattatct tgaatagaat ttataatctg gatggcaact ttcagagcgc
    13621 gtattccatc ttcaccacta acgcgcggcg gttgaccagt tagaattgca ttgacgaagg
    13681 actccaattc attataaagc gcgttaccct gttcggtttg ttggacgcgg tagaaaatcc
    13741 gtcggggggc accgttcaca tccggcactt ccataatttg agtagtatca tcaggcaccg
    13801 gtccggcatc gcgagataaa gaatagattt cggtggaacc ggctaacata tcaatcgaga
    13861 aataagcatc ccgctggaaa attcgcaact tgcgcatttg cttgttcgat acgcggctgg
    13921 cagtaacgtt agccacacaa ccgttggcaa agcacaaacg agcattggca atatcaatat
    13981 tatcggtcag aataggcgcg ccggaggctt ccacctgaac tatcggtgcg gtgactaaat
    14041 ccagcaccag atcaatatcg tgaatcataa gttccagaac aaccgcgacg tccgtaccgc
    14101 gaccacgaaa aggtgcaatc cggtgtgact cgatgaacaa cggatctatt tgatactgct
    14161 tcaatgcgcc caaagccgga ttaaaacgct caacatgtcc aacctgtagg acgagatttt
    14221 tcgcgcgagc aagccggata atttcctcag cttccgcaac tgtctccata aaaggttttt
    14281 cgcaaagtaa gtgtcgtccg cagtcaatag cggcttttgc cacgctataa tgggaatgag
    14341 tcgggaccac gatcgaaacc gcctcgcaat cgtccagcaa ttgttccagc gctggataag
    14401 ctcgaacctg aaattcgttt gatatatagg atgcccgctc agggttgttg tcaaagaaac
    14461 cgactaattc ggcattgggc aaattttgta aatttttaat atgccagcgc ccaaagttgc
    14521 cagcacctac tacaccgatt ttaagcataa tatatagaca ttctcctaaa ttgttctgta
    14581 tcgaataaat tttaaaaaac ttgattttgc ccgtcaatat aatgaaattc ttgattatgg
    14641 aaaaggtatg tttcattcaa aaaaactggc taagccaaaa gttcgcaaat ggaatatctt
    14701 tattgattct aattatggtg atcaatttat gggcggagct gatcgccgaa gtcccccaag
    14761 ttgtcattgt ccattggaat gatttccaca gtactaacct tccttataaa tccagtcagg
    14821 cagactatcg agtaggtggt tacgctaatc tagctggata cttcgattca cttcgccatc
    14881 ttcatcccca ggcgatcatt ctgaacgctg gcgatgattt tcaaggctcg ccagtttcga
    14941 atttaactcg gggtctatcg caaattttga tcttgaataa agtccaacca actgctttca
    15001 cactcggcaa tcatgaattc gattatggta ttgataatct tcgaaatgtg atgacctatg
    15061 ccaacttccc gatcctgaat tctaatctat ttgactcaac gaaaaacgat ttattagtcc
    15121 aaccttatac tatcattcaa tctgggaatt taaaagtcgc tattattggt gctctaactg
    15181 gagatttgtt caccctggct ttgccgcaaa atctcaaagg catcggtatc cttgacccgt
    15241 tgacccaaat ccgccaatat gtgaacgaag tcgaacctca aacggattta atcgttttgc
    15301 tgagccattg cggttttgaa gaagattcgc ttttggctat gcaacttgcc ggtgtggatg
    15361 ttattattgg cggacattcc catacctact tgcaccagcc taaaaaggtc aacaatatct
    15421 tgatttgcca agccgggtcc aatggtcatt gtctgggtta tttgaaagcc aatatagatc
    15481 gaaatcagaa aatcatcacg tcctataatt acgaattaat agaaacccgt ctcgggagag
    15541 taaaaccgtc accaattgtc gccggagtag ttgactccct tgaggcttct atctctgcag
    15601 aaatggataa aatcattgga caattgataa ccgactggca ccgcaatagc cacggtgaat
    15661 cgaatctcgg caattggatt tgcgatgtaa ctcgtgaata ttttaagact gatatcgcct
    15721 ttatgaatag tggcggcatc cgtaaagact tgaaggcggg accaattcga gtgcgtgatc
    15781 tctgggaaat ttcaccattc gataacacga tcgtaatagt cgcattgacc ggagaacaat
    15841 tgaagttcat cattcagtat cacgtcgaaa acccgcgtga ccttttacag gtttctggac
    15901 tttcttatga atttaatagt aaacaaaaaa agttagtaaa attgaccgtt gcgggcaaac
    15961 cggtcgacga tcaaaaaatt tatactttcg ctacgaataa tttcgtgatt agccaattcg
    16021 aacgtttctt cggtctagcg ccttccgcgg taaaaattac tcctaccgat atcatcgggc
    16081 gtgacattct aatcgaagcc atcaaagctc aaccggttat taataatcac acggaaggac
    16141 ggattattga cttagcaaat aaaaaagaag aggtaaatta tgacatccag tagtcgcatt
    16201 ttgtatagtt acataatttt gggaatcgcc ctagtggctt ttggtatcgc aaccatactt
    16261 ggtcactttt atatcggctt aatttgctta atgctcggaa tgattatatg gcttatcgtg
    16321 acatttgctg gcaatactga ggggcagagc ggtcaaattg gcgtggtaat gattacagcc
    16381 ggatttatcg gtggattaat aatttttatt ggaattggat tgcgtccgag cattttcggc
    16441 ggacataaat tatatattga aggaattgta acggcattag tggtattact gatttttatg
    16501 gcaatcggat taggttttat cagtctggcg aatcttgaga aaaaaatggc ttttttcaat
    16561 aaagaattga agaaattcta caagttgtat atgaaagaag ataacgaata aaaaagcatc
    16621 cataatactg gtctttactc taccttgttt ggcactgacg caaaatctgc aactccatta
    16681 tgattttggt cgtcaacatt taacctcgac ttacgagcta tataaaaccg accgactggg
    16741 tgcgaccttt ctctttgttg atttcgatta taataatgaa ggttcgtccg gttcaagaaa
    16801 tgcttctctg gcttacggtg agatagctcg ctatttccaa ctgccgaaaa tgaatgatct
    16861 ttcggctacg ttacaatata atgatggctt gacaaatagc ggttctttca attcggtttg
    16921 gcttggcggg ttacaatacg tttttcaaat tggaacgcaa aattttccaa ttgactttct
    16981 tctgcgtaaa gaactaaata ccgacggatt aacctttcag ttgacttatg tttggagtta
    17041 tatctggcgc aaattggaac ttagtggatt catagacatc tggaataccg gagcagatgc
    17101 ctatccgcct cgaaaaattg tcattttatc cgaaccccag ttttggtaca ggctcacccc
    17161 ggcactttcc gttggaggcg agatcgaaat tagttttaac ttctcaggtg cctggcatac
    17221 taatcaggtt ttttccgagg atgtaatatt tattctaccg acacttggct tgaagtggct
    17281 gttctgagtc cgctttcccc aaaaatcatc agattaagaa aaaaatcgtt cggcgattta
    17341 aaataaagca cccaattttt agtcgctcgg tctgggatat ttacttaaca atatctgaaa
    17401 attgtcgagg aacaattatt cagttgccgg ttcttcagcg acggattccg atttactttt
    17461 tttctggata ataagtagaa cagccagaat taacagacct atgactgtga ttacagccag
    17521 gattttgaaa attagtttta agtatttcat atagcaccca tagaattata attttttaag
    17581 taaatcagcc gcttctttct gcataacttt atcagaatcg gattgcgccg gaatttctaa
    17641 agccaattgg agattctttt tagcctgatc tttcttcttg agttttagtt gagtcttacc
    17701 gagccagagt agatgacgaa tttcctttgg cttaagttca tgtgctttct gaaaatactg
    17761 ttcggcttct tcgaaggagg ctttgggcgg cgttgcataa atcaggctgg caacctggcg
    17821 ttcagcccaa cttaaatcgg ctaattcaaa atgccagcgt cccataacat ggtaattcga
    17881 atcctctttc ggatctaatg caatggcttt ttcagtatgt tcacgcacgg cataggagtt
    17941 ggtgatcttt tgtttggtgc cttccagttc gccgatttgt ccgattaaaa tggcgtagta
    18001 ctgatgtccg cctgctactt ccggcgccag ctctacgcat tttttggcat actcaaagcc
    18061 ggggtagaga ttggcttttt ggacttcgct tacgccgggc tgttgatcag cgatatcaaa
    18121 ataagctctg gcgagtctcc atagtacttc tgggttatta ggttcaattt tttcggcttc
    18181 gcgcaatttg gtcatcgtgc cctgataatc tctggcatcg tgcaatttat ctgcttgttc
    18241 gagtattgat tttactttag ttgactgagg agtcagataa atcggtaaga cacataatcc
    18301 cagaacaatg aaaaacactc tggttgcatt agagaaaaat aattttcgca taggaacctc
    18361 tgtttatttt cttgatgaga aagattatga ccgttgttta aaatgtatag atgaggcgat
    18421 atctccggga ggctaaaata tttcagtaaa agtgaatttc aattcacgat tcataaagtc
    18481 cacgtctttg cggttcagca ggatattatt gaaatctagt tcaacagtga acttatctac
    18541 caatctccag cgaacaccgg catttaaata gccccaccct ttaccgagca gcggtaattc
    18601 cgtgtgattg tcgtttagag ccgcatcata ttcgataatc aaagcaattt cggagttcag
    18661 gtctttatct atacccagga aaaaattggg gtcggcatcg ccatccgttt tctcgatcgg
    18721 attataatta attccgccat gaagacctaa attgcctaga aaattgaaat ttttgctgac
    18781 gacaacatag aatccagagg ctttggtttc atagcgcttg agtgaatcaa tataatttcc
    18841 atgaccttgg gacgaaaaac caaccagtaa ggccggaaat ttaacgctct cttcgataaa
    18901 gcgatatttg atctcaacgc ccggctgatt gtaccaatta atcttctggt taccaataat
    18961 cccaccaccg ccgtaagata ctccgaacat aaatctttcg aaaactccta cacttatttg
    19021 acctgctaat cctccgcctg ggaaaagatg acaatcaacc gcataggaac cacgcggcaa
    19081 tataccagca gtcggtatgg tgaccagatt aaccggctga tgaattccta aatcaccttg
    19141 agccgataga ctgccagcca gcaccataaa aaagacaaag gggaaaaatt gttttctatt
    19201 cataaccaac tctcattttt tgttgaatat acattcggca aagctgaaaa tcaaatgaga
    19261 tttatagcct cgtcgataaa attcagataa atgatatata tcaaacaaat taagtttgca
    19321 tttgatagtt tgggctaaac tttttatatt taaacgtttt taagtattct atgcagtttg
    19381 ggacaaaaaa agtgaaaagc atccagaaac taattcagaa agaaattgcc aatcgaggaa
    19441 gtcttattgt cccctttaat aacgaagcat tactgagcat tttatttgtg gcattaacat
    19501 acatattggt ttaaaaagtg attttcaatt taaaaacaca tagtatcact attgaaatta
    19561 tgacctttga atctcggcag gaaggggtat ttttatgaaa ggttttggcg ctgttcttgt
    19621 tgcgattagc ttaattttgt tagtgctcga gcatttgaac ttagttgatg tagtcggcat
    19681 tatcggtttg ctggtcggtc tttatgcgat aattttccct gataaagccg aagaattata
    19741 taccaagtta aagtcaggca aactgaagta accggcctca gatcgctatt ttttaaaaat
    19801 agtgtaattc ttaatctatg aagccaagtt ggtaagattt aattgcttgt ctatttatct
    19861 agattaatta aatgtttgat taataatttt agctaaaatg ccaatttacg agtttgaatg
    19921 tcagaattgt gggcgtgttt tcgaggaatt agtcttctcc aaaaactttt cagaaagcga
    19981 aattacatgt ccgcattgcg gatttaagca agcgaaaaaa ttgatctcgg ctccggcgct
    20041 aagtaaatca tccgcctgct cttcttgcgg tgctaaatcc tgtcccagta gttgtggttc
    20101 tcgaagccat taattcatta tggcaattgc cggaactcat cttttgccga aataaataag
    20161 atattacgga tcaaattgag ggtttatcca gcttgaaatg aaaagtagca aattgagtga
    20221 taaattgtct ttagcagttt aatttccccg tataagcatt aaaaactaac tcagttaatc
    20281 actattattt gtatttttat atagactttt agcgtgctcg gctgaaacag taataccttt
    20341 tatcattgct gaactaaaac cctcgtgttc catctgattc aagccggcaa tcgtacagcc
    20401 acgaggagtc gtgacccggt caatttcata ttcgggatgg ttggcatgag ttaggatcaa
    20461 agtcgccgct ccgcgggcag tctgggcggc aatatcaatt gcttcctggg agtgaaaacc
    20521 aatctcgatg ccgccttgag aagccgcccg aatagctcgt aggaaaaagg cgattccaca
    20581 agctcccaaa gcagttgcgg cagtcatttt ttcctcttcg atgactatag ttttgcccac
    20641 cgtatcgaaa atagatttgg taattgcgat ttcagcttcc gaagcggtat ctgatgccag
    20701 acaggtcatt gattcccgta cggctattgc agtattgggc attgcgcgca cgaccgcgat
    20761 gccagatccg acctttttaa caatttgaga agtattgact cccgtcacga ctgatatgac
    20821 gatatggtgg ttgggtttta aatggggagc gatttcaatc agaaccgaat tgacctgctg
    20881 tggttcaacg gcaatgataa taatttttgc ttgacgaacg gcctggcagt tatctttttc
    20941 gataataaaa ccacgttttt gcatatcatc cagcaagtga atgcgacgtc gagttaaaat
    21001 aatctccgca ggcttgaaac ggttagagtc ggccaaacca ttggctattg cagtcccaat
    21061 atttccagca ccaatgattg caatttgatt gtttttcata gtgaattctc ctttagcttc
    21121 aaacggatgc ccctgaccag tcgtatattt cacggttaat ccgccgatgg ataaacgctg
    21181 tctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccctg accagtcgta
    21301 tatttcacgg ttaatccgcc gatggataaa cgctgtctgc cttaatacct ttaacttggt
    21361 atttggtaat cggtaatccg taagtagttc ttgataaatc tgcatattta cagtataaat
    21421 tttcacaaca ctaaatttta tgataataaa aagtcattgt caagtagccg gaataaatca
    21481 gattctcaca ttgagtcctt ttctcatagt tacattatcg gtaaacaaca tcaggtttaa
    21541 ctctccattt tgactattaa atttcatcta atgacgcgga ttgccgagtt ttagcgagag
    21601 aactcggaga atatccaccg gtgataacat tgccagccgc ttgcgcgcct ctttaatatc
    21661 aaaatgatct acttcgcgca cttcacgaat caacggcaac ataacagctg agacaagcag
    21721 acggatcaaa gcactgagca ttaaaactag tgctaacgcc gaaaagcggt gaaattggaa
    21781 cggtaagtca gcaatcaacc cgccgatcaa accaccgaga aacatcccaa tgccattaaa
    21841 aatgttgtag taagccacac aaatcgccat ccgctggcga gtaaccgcat cgtaaatgaa
    21901 attgacggtt gtgagattga atccagccca ggcaaatcca ctcagggttt cgactaagat
    21961 caagtacagc accgctaaaa cgggacgatc gataaaaaat actgccaaaa cccataatgc
    22021 cggaatgatc gggaggcaaa aaccggtgag gcgcacgacc cgcaaattgc cgaagcgatc
    22081 ggcaaagcgt ccccaagcgg gcattagaag catattgacg aacggcatag tcatagtaat
    22141 aagagtgaac tgaaaataat tgaagtgcaa atcctccagt ataaatactg caaaaaacgg
    22201 cgaagaaata tatacggcaa attgcattag tgcaacgtaa gtaacaaatt taccgaaatt
    22261 gctctgggga atcttgcgaa tgaactgcca aatcgtgaag taatattcct tctgaggatg
    22321 aaattccggt tcgtattgac gttttaacaa agctgacgaa agagcgcgtc cggtaaaagc
    22381 aatcagaaaa atgatcgcaa agcccgtaaa gggttgcccc aacctgttga aatgttgcag
    22441 gattaagcca gcactaagca tagcgagaat agtggtaaat cctacaaccc gattgcgaag
    22501 gccaaaataa gtcccgattt ggcgcgggat gatgtcgccc atccaggaac tccaggctgg
    22561 gccaacaata ccgccgaaaa ttaccatcag actataaaaa accattagtc cagcagaaat
    22621 caccatattg tttagattat aattaaaatg caaccaaccc aggaaaataa tcgggaacca
    22681 cattaaggtt tgcaaagaag tacccaccca agcaatggtt ctacggtgaa tacctttctc
    22741 taaaaatttc aacgtcaaga gttggatcaa ggtccccagc atctgtggta aagaaatcaa
    22801 aattccaatt agagtattgc tcattccgag caaaagggca tacggagtaa tgtaacgcaa
    22861 gccaaatccg tcgaacaaac catacgcggc gccctcacgg atgctgatcc gactggcttt
    22921 tttcttaaga tattcaattt tacgagcgtg gtttccagtc atcgggcgca aatttattga
    22981 gtagccaaat tgtatccaag tcacttgtaa acaataaaaa tttatacgtc atatccctaa
    23041 aatggtctat tgatagacag atttttcctt tgataatcgt tggtatattg gtaattttgc
    23101 acgctttttg tcagggtgca tagctcagtc ggtagagcaa cggcctttta agccgtgggc
    23161 cgcaggttcg atccctgctg cactcaccaa tccaaattta tcattgatat ctaaatttac
    23221 gagcaagtcc cctgtttgcc ttattttcca agtggtaccc aaatccggca ccgagatgca
    23281 agcctcataa caggttgtca ataattcgaa ccattatggg catcttttcc tgacagttca
    23341 attcaacttt gggtggttgt gttaaaatag taaaaccaat ataatccgaa agggaaaggc
    23401 tttgacgtta ctgactttag catactcagg tgaaccgttt aaagctttta ccacttaaaa
    23461 tcaaatagaa ttggctctga attcaaggac gaacaatttt gcaggtcggt ctaaattcca
    23521 ggttttagcg accgataacc ctgttcggta ctcactccat cagattgttt aattcagacc
    23581 gtcactaaat tatataagat gagagccggg taaacgtctt cgccctgtct tgtatatttt
    23641 taatttccta ccgaaataca gttatccgat tttagagagt atgttagcga tttctgtata
    23701 attgtcagca taaaaatgta atttcggcat atcacgatcg ttccggtcgc accgaaaacc
    23761 gatagttacc gctcccgaag cgattgcggc ttgaatacca gtcattgaat cctcaattat
    23821 tacgcactct tccggtcgaa ctctcagact ttcgaacgct ttcagatatg gttcgggatg
    23881 aggcttagcc tgatatacat catcagccgt aataattaaa tcaaagatat tgcgcaccgg
    23941 tgtgttacga aatacccact ccaaaaaact ggctgaagtc gaagtcacca gcccagtgcg
    24001 ataatggccg tcaatgcttt gccagaagtc gaggaaaccg ggcaaataat caaccccctt
    24061 gttgaattct tccttgatat agcgccgccc gtcttcgatt aatttatgaa catcagtatt
    24121 aatctgatac ttagccatta tcaattgata aaaatcaccc tcggttgtcc ccttaaaata
    24181 tttccaatct tcgtccggaa cttttatccc atactggtca aataagcggc gttctgcgac
    24241 catatataaa ggttcggagt taataacgac cccatcaaaa tcaaatatta cggccttaat
    24301 tgccatcctg ttttaatcct aatcgtaaat tactacatta actatcattt tatattctgt
    24361 actgttaaaa tcacaagtaa tacgccaaaa cataaattat tatttctaac caattctcct
    24421 gattgtccca taattacaag ttactattgc taaaaattaa ttattgctct cgatcggaag
    24481 ttccacccgt atagttgaac cgctttccgg attattgaaa gcagaaaatt tggctttata
    24541 gctactcaat agcatataag cattgtataa attatactta atgagggctt tctggtcgga
    24601 agcgttttcg ttttcaggaa tctcatgata aaaactaggg gtaaaaagct cctcggcatt
    24661 atccggaata ataggctgtc ccgtggtagc aatttctata taaatattat cctgggatat
    24721 accagtttcg attttcagta tttttcgagg agaatcgcgc attgattcaa cacagcaatg
    24781 gatgatattc atcaaactct gcgaaaagtc actataatgg ccagtaatca gcggcagatc
    24841 gcgtcttaag acgatttcct tttcaatctg ggttttaaag aaagattcgg tctcaagaaa
    24901 taacagttca tttctaagca attcacttaa attgatcgtg attttcttgt tctccagttc
    24961 gaggttcatt ttatctatga aactggtcaa tagagattct atttttcgta cgttgtccac
    25021 aatgatgtcc ggctctttta actccggcag acgggttttg agcatctggg cccgtcccat
    25081 taccaccgtg agcgtatttt taatattatg gataatgcca ccgatcatta aggtcagcga
    25141 cattaaccgt tgttgcttaa gccattttac ttccagagct tgtttagcca ggctaaggcg
    25201 tttatattcg gaaatgtcac tgattacggc gaaagtagtt aatttacccc gatactcgat
    25261 ttccaccgga ctgacttcca cttcaatcgt atgaccatcc ctacatttaa aggaagtttc
    25321 gtatcgcttg ggaacggttt taccgattgc ctgccgacgt tcgcgctcct tgagtatttc
    25381 gactccgcgg ttcgtcaaaa tattatgaaa ctccataccg attagctcgt caaccgtata
    25441 accaagcatt tcggctagtt tattattaac aaatataaag cgctctttat ggcagatagc
    25501 aataccttca ttgattttgt caataaataa atgatagagg gacatggttt cagccatctt
    25561 ctcttcagtt ttctttcggt gaagggcaga ggtaatgata ctggcaacaa ttttcagcaa
    25621 ttctatttca ctatccagcc accgccggga agtttttgat accgcgacta ttagtaatcc
    25681 gaaattagta tcgttatata ccagcggtgc aatcattaaa gatttgattt ctaattgctg
    25741 taattcctgc ttttctcggt tggctttggc cggtagatta ttaacgtcat taattatgac
    25801 agtacctttt aaactcaact gatttttcaa ccagggggaa tttgctaatg caatattttg
    25861 caaaagttta acggaatcct ctgatttatc ttcgagccca tatattttca gcgcctcttt
    25921 tgtgtccggc agtaatgccg ataccaaaca atattcggtc tgataaaatt cgcccagatc
    25981 ccgtagggat tcatttattc tgctgtctat ttcaactctg ggtacgtcaa taaatcggag
    26041 tgcgatttta attattaaat ctttcttact aatcatattg tgctcacttt tcattctgca
    26101 gggaaggtat tcgattttat taaaatccgc aaataggata aatgttgaac agatttgctt
    26161 ctgatactat tgatgttcct atttaaaact atactggctt agaatatttt caagatcact
    26221 cgcagaaata aattggtggg ctttaaggcc gagtcgctcg gcgccagcga cattttcctt
    26281 aagatcatca ataaataggg cttgttccgc ggttacatcg gcttttcgca aagcatattg
    26341 ataaatcgcc gattccggtt ttgtcgcccc gacctcaaat gataaaatca atccatccat
    26401 attctccaaa atattgaatt tccggcggat cgcccgaata tggagggcat tggtattgga
    26461 taaaagaaag agcggtactt gccgacggag tcttaaagca atctgccaga cgtccgtatt
    26521 caactcgaaa gtagtctgcc aaatccgttc gaggtcatca ataccgatgc tgtgatctat
    26581 gtggtaaata tttttgactt ctactaaata ttctgcaatg gttaattgac cacgctcaaa
    26641 tttcttctca agtgcgagaa tcggcgcttg catcaccgct ttttccggaa tacggaatag
    26701 ttccgcaatt tggggaatag cagattcgag attgactttg accagaacat taccgagatc
    26761 aaaaaaaacc gctttaatta ggttgtttgt gcgtgacatt ggttatataa ttatcgtctt
    26821 aggtaatttc cggctattcg gcaactactt ccgtgaagaa tttgccgatt tcgacgtcgg
    26881 tataactttt tactgccgaa ctgaggtagg aatccagttt tttggcaatt tcatcatcta
    26941 tcaagtgctt atgctttagg actgcgaccg ccatcgaatc acaagcttcc cggttaccac
    27001 tcagcacctt tatagcaata ccgatcggat tattttcatt attccgcaga ccaattccac
    27061 gcacaccttc cataccgaac ttagaaatac cgcgccctcc cattaaagcc ccaaaatcgg
    27121 tatcaaattg ggcgcggcct ccgattagtt tcgggtggag ggaaatgcta tggaaaaccc
    27181 gtcgtaagta atcgtcgctc ccctcgacta attttttata cattattgcc agattataaa
    27241 ttggcattag ataagtcggg gcattactgt tatcagttgc gaccggaata cgctctttgc
    27301 cggagtatag ttttattttt tcaaagattt tttgctgggc gggatggttg atctcggtat
    27361 aattggacaa atcgtacttt aaagccttag ctatcgcgag cattccggcg tgagcccctg
    27421 aattggcgtt gtgaagcgca gtaggtcttc gtccttgaat agctaattgt tcataagttt
    27481 ggcgatcgag gggcggatgc actccacata gtaaattatc tatactgagg tccatttttt
    27541 tcaggatgga attgagcacg tcaagatgat aatgctcacc ggtgtgagtt ccgcacatta
    27601 aagccagttc ctcatcgtta aaattaaatt gctccagcgc tcccgattcc aacatagcga
    27661 ctgcttgaaa aggattagca gcggctccaa tgaaaattgg atattcagga tcccctgccg
    27721 aaaataggat ctcgccattt tcatctacca caactgcata accaatatga aagctgtcaa
    27781 cagcatttcc tcttactaac ttacttacca gtaacatacg aaaacatctc ctttatctat
    27841 tttcaacaat ttcaagtaat tctcttaaag ctgagtcaaa cccggtgatc aatgcctgac
    27901 ccagtaatgc ccgacccaca gaaataaact ccacatctat caattccaaa agacttaaaa
    27961 tcaggcgctt atgcaatcgg cctccgagtg aaactctcat atgattgcga attgccaaac
    28021 gcgaagccgc aacgatttta ttcagctcgt cggtgtagga tacctgtaat acctgggtga
    28081 ctaatggatc agtagctagt tccacttcgt caactttcaa acgataagcc gcttttaagc
    28141 cgcgtatctc cggtttcaac cgtaagatta aattgaaatc ttcggcactc tgcaccgttt
    28201 ctatgagatt gtgtactatt tgagaatgag ggtcaaccgt caaatccgat tcgtctaaat
    28261 taataagggt gatggaatct ggtttggcag acatagctcg ctgaatagct tgattctcaa
    28321 tatttgcccg gatattcagc cgacagcggc tcatagaacg cagtgctgta atgttaatca
    28381 ttccttttcg ttcaggtgta ttcgtgaaac tgattccggc cacaccgacg gcttcgatga
    28441 aacccaataa atttagcgga ttacccagcg gaatatccac tatccgacca agttctataa
    28501 aggggtcaag atttatttct aagtaatgca tatttttatt tcaaagaagt tacttccgaa
    28561 gcaaatgtaa gttcaacttc gtttaaaagg ccggattcga gaatctcggt cagggctgac
    28621 aaagtttcgg aacatatatg gccgatcgcc acagcgtgcc ctccgcgact ggcaatctca
    28681 atcgcccttt caaattgcgc ctgaatttcg cctttatccc taacattatc aagaaatacg
    28741 gtccggagtg ccgtcgggat ttgattgcgg atggcaactt cataagcgac actttcggcc
    28801 gaagtcatac tatcaataaa atacaatccc ttgcgtttca gactattaat cacgatttgc
    28861 atcagttctg gatcactcgt aaatagcgag cccatatgat tattcatacc cacagcttcc
    28921 ggtaattcgg cgcaagccat ttcaactcgg tgactgattt cctccggggt cataccggtt
    28981 aagagcatat acttatcttc gccgtaacta ttagaacttt gctctggctc cattggcatg
    29041 tgaataatca cttctttacc cgctagcttg gcgtttcgag ccacccattg tgaataaggg
    29101 tgaccgggaa tgacggaaat cgtcaattta gtcggcaact ttaaaaaatt acttactatt
    29161 tcttggttag tatagccaaa atcatcaata ataatcgcca atttgggttt tactcccgag
    29221 ataggcattc gggctggata aggtctatcc gtcttgatga acgccagagt tccgatcgga
    29281 atatgttgaa aatcaacgaa aagagtaaat ccggttccct taacgaggtt atctattttt
    29341 tttaccataa aaccgtgttc attaagataa gcactaactt gttgagcaag tagttcacaa
    29401 tccctactgg ctggtaattc aaatatgaga gacactctat cggtggcaac tgaggaactt
    29461 ttaatttcct gacaacctcg ttctagcaaa aaggggcgag caacatttat tagcctactc
    29521 ttaccttcag aaaaaaggtt agcccgagaa ggagccattt tcggtaaaaa atagacaagc
    29581 aaaattatct gcataccaat gacggtagaa aacagtgcat tcagtctctt ttcgtctcgg
    29641 tgacgttttt cgactatttt agatggtttt tttaaataat tgctcgttcg ggtcatagta
    29701 ttttaactgc gtaaaactac taaattccga ctgattctca aaattgtgct acaaaaaatt
    29761 atccacttcc tttagtggca aactacctaa cggttcaagg ctgttcaacc gttctccttt
    29821 atttgctcat gatgcatcga cttttcatat ttacccaacc gaaaagcaaa tagcaatccg
    29881 caaaatccaa ggatcgaaaa aatccacatt ccgagattat aaccacccgg attatctgcg
    29941 ctggcaccgg taaaatcatt tgctagaccg attataatat taaaactgaa cagtccgata
    30001 ttttgaatca ttgtcattag accataggca gtgcccagtc ggttattgtc aactatgatg
    30061 gcgaccattg gccacattac tgctggtacc agcgaaaagg caattcccat catcgccatg
    30121 ggaattatca aataggctgg aatactcgaa tttataccga agaaactaaa ttttatattt
    30181 attatatcat gaaaacccag aggagtcgcc agatcaatgt ggtaagccat tatcagatac
    30241 accggaatga tcaacagtga gcccaacgcc atcaaccagg cttttcgacg agtgcgatct
    30301 gccaccaatc caaataacgg cgtaaaaatc atcgcggcta aagtcaacat actggataaa
    30361 ttaccgccaa cttcgcgaga cgtaccatgc gcctcctgaa aatatttaat agcaaaagtc
    30421 tgaaaaggaa acatcgcaga gtaaaatgtt acgcacaagg cagttatgta ccagaaaccg
    30481 gctccaaatc gaaaaacctc gcgtaattca atcttgtcct gcttactttc ttgaggcaag
    30541 agcaaccggc gagatgccag taaatccagc aaacaataaa taccgataaa aaacaatgcc
    30601 atcatcccag ccacagccgt taccaggagc ggtttttgcc agctggtata aagactacga
    30661 ccccaagtcg gcgaattgag cgccagaaat gaacccaacc gggcaaaggt tagattcaat
    30721 ccgaaggcaa aggataactc cttgcctttg aaccagcggg ctaaaatcgt tgtaatagcc
    30781 acaatcattg actccgcacc taacccgaaa atcagtcgtc cggtcgccat taataataga
    30841 ttgccgctca atgcggtaat aacggcgccc aacataacca aaatcgtgaa aattagaacc
    30901 gatttacgag ttccgatccg gtcaataatc agtccgccga ttaaaaccat gataatgtta
    30961 ggaaagctat aaatagcatt taatagcccg atattggcat cactatagcc cagctgagcc
    31021 ttcagaagat cagctaatgg gctaatactg tcatagatat aataatttcc aaacatcgcc
    31081 atactgataa aaaagaaaac taaccagcga tatcgggcgc taggcggctt cagttcgaca
    31141 tccgttccta ttttgtccat ttccagtcct tacaaaaatt cacacaccat cataacaatt
    31201 tagcggcagt taatctaagc ataccctatt tcaatacaaa gaaatctgac gggatattgt
    31261 ctggattgct ggctttattt aaatttcggg cgatttttga ttcacgaaat aattacgagg
    31321 agttatagta atggcttatg taattagcga agactgcacc gcttgcggga cttgcattga
    31381 cgaatgccca gctgaagcaa ttagcgaggg tgatatctat aaaatcgatc ccgatctatg
    31441 cactgactgt ggggcttgtg ccgaggtttg cccctccgaa gctatacatc cggcataatt
    31501 aaatttgccc tcaaggcttt agccggatta aatatttttg aattagagtg aattcacgtt
    31561 tttgtttatt aattttgggc agaatttggg cttagggttt ttgcagacaa ataactgatt
    31621 tagaccactt acaaagtttt tccgattggc tttaaaccac agaatgctat ttaaaataag
    31681 actgattata ataagcctta taaatccttt atcaattcaa taaatgcaat cagcacctat
    31741 ttcacttttt gacaaaattc ccgagataag ccagtccctt gcaaatgggc gacctcttgg
    31801 tttagtattt aatctcgaat gccttatgag ttcacgccgt tggcgggcgg gcgcgggtct
    31861 caccgagatc cagcgtcaca ttcattgggt tgccgactta ccggatataa taaccgtcat
    31921 tctaagcgat ggttcgctta atgagctcga aactctattc gacaaccaca agataatttt
    31981 agcacccaat aacggcactg aaatttacgc ggctgatttt tcctggaatt tacctaatct
    32041 taacttgatc cggcaggaat tgaccgatat ttattgtgcg gttcgtgaat cccttggcgc
    32101 taagcttcag ccggaatatt taggtttttc tcaaagcgaa ctctggctca aggtttcgcc
    32161 gggggcggag gcagtccgaa aatcattgat tgaacagttc aaactgcaga acccggcgca
    32221 cctccgtctc ataacccaaa ataatcagac gactatcgtt cccctcataa aatgggatcg
    32281 cggtcaagtc attcaaaagc tgatggattt gataccccac ccccttaatc aaactccttt
    32341 agtaatttat ttcggagtag aggagaatga cgaagcggct tttcactatg taaatcaagt
    32401 cggcttttca gtgatcattc ataaccgtat tccccgtgct acggaagctt gctattatct
    32461 gcgtaatgtc gctgagctaa gtaaattttt gttctgggtt cactcccgct aaggttttcc
    32521 cagaaaataa aaatcctcca cgcaggagga ttaatgagca atttttgatt tgataatcag
    32581 gctaaaacgc tgactatttt cttatcccgt cctttacgcg agaatttcac tttgccgtct
    32641 attagagcaa aaagggtgtc atcactgccg attcctacat tctcgcccgg atgaattttc
    32701 gtaccatgtt gacggataat aattgtacca gccgatacct gttcgccacc aaaacgcttg
    32761 acacccagcc tttgaccgcg actgtcgcgt ccattgcgtg aacttccgac gcctttttta
    32821 tgagccatac ttactactcc tttttctctt gttcggcagg ttcagccgat tcgcttacag
    32881 ttttagtttt cgcacgactt tttttaaccg gcgcggcggc ttccggtttg gcaaattcgc
    32941 ttacttgcag aacggtatat ggttgacgat gccccttttt tacgcggtat cccttgcgtc
    33001 tttttttctt aaaaattaca accttactgt tacgcttatg ctcaagaaca gtggtttcaa
    33061 tagcaaaatc ctccagatag ggtttgccaa ctttaatatc attgccggta tgtataaata
    33121 agatcttatt cgccagaaat ttagcgccaa cttcaagatc catcttggga actgcgacgt
    33181 ttttccctgg ttctaaaatc acctgatcac cggcgatttc agcaattgca taattttggt
    33241 tattcataat tctctcgtga ttactatcaa aaaaggctgg gaaatttaga tataaagact
    33301 gaataatgca agatgccggt cggatatttt tgtctgatag ggaagactaa gacaggagtt
    33361 gaggctacgg gctttgtctt agtcattatt cttgcctcga ctttagcctt atatttcttt
    33421 actaatgaca aatgactttt caacgttcat ccattgtgac aacggagatt gccagaatac
    33481 ggtcgcctgc ttcaaggcgc ttgaccacat ccattccaca aatgacctct ccaaatcgcg
    33541 tgtattggta attcaattgc ggctgatcag tcagagtaat aaaaaactgt gaaccttcgg
    33601 tgtcatattc tgtccgaacc attcccacgg caccgcaatt aaattcaatc ggcgatcgtt
    33661 cagttaaaag cgtataaccc ggaccacccc aaccgtcacc acgtggatca ccaccctgta
    33721 caacgaaatt gggcacaacc cggtgaaaat tcaatccatt gtaaaaacct ctctcaatgg
    33781 cggatagaaa agcatcagta gtaaacggcg cataatatcc atctaagcgg atgatgatat
    33841 taccgcattc ggtttcgagc aagatcgtcg gacgcagtcc atatttttcc aacattttta
    33901 aatttgaagg gcgcccggca cctcggtaat cattgattat ttctggcaca ttagcttcat
    33961 aaatttcttg taaaatttga tatgccaaat ctctaatgcg aaaatcgcga gcgttcaata
    34021 acggctggat gaatggcaca gcatctggcg gctgtcgaac ggctatcgtt tttaatattt
    34081 cgataacagc gtcagcgtat tcggcatttt tgaatttttt cagaggagga tagagcaaag
    34141 gcactaaatt ttcgcatttt gtggtagagt cacgcataga ttcggcggcg acggcgatct
    34201 tcaccggatc gtcacttttt aaaccagccg caatataatt acagaattgt tcgcagggga
    34261 tgctatcctg tcggcaaaat tgctcagcca aattgatcaa accgagatag gcaggggtag
    34321 atattgctcg aaattgatgc cgggagaggc tgtcaagaac cgcaagccca gatatttggc
    34381 ctgaggcggc aatgccttcg gcaaatgccg gtagcaaatt ggtctcaact ggccagaggc
    34441 caaaattcgc cagtgtctct tgaccaccgc gtcgactcag aaattttacc agggcactcc
    34501 gtactgccct tgacggatga tgcgctagag tccgaatgtc gtcttggaga accgggatca
    34561 attccagtga atcgggcaag ttctcaatag ccgttgctac tacctgtgga aaggaatcat
    34621 tgaaaacttg atgtaaaatt tcaggatcgt gagtgacaat gcgaatgttc cgtgcggctt
    34681 caaggcgaac ttctggaaca ctatcggtca ataattgcgt ccagatttca gccttgccgg
    34741 agaaattcac aggagccaga caagtgacaa tttttacacg ggtggcttgg cccgcagttt
    34801 taagggcggt tttgaggatg tcaacgtatt cttgcatcac agttggattt tgtacccggc
    34861 tcaaggctaa agccgattga taagcttcgg aatgccgatt gctttgcaaa tgggaaacag
    34921 cataatttaa gctggctgga ttgtaaattt ggcgctgaaa ataataaatc agagcctgtc
    34981 cgagatattc tgaattagtc tcaagcagaa aattcaaggt cgagcgattg ccaaactggg
    35041 caagcgccaa gacagtttgc tgacgcactt cggtgtcagt ttcgctcaac aagtgctgaa
    35101 cgagcagact ttccgcagtt tggctggcaa gttggccaag ggcaaaagcc gctccagtcc
    35161 gcactgttgg cgactgatca tctaacaaag
//
LOCUS       DAOOMU010000009        34199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1037_length_34199_cov_19.607750, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000009 DAOOMU010000000
VERSION     DAOOMU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 34199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34199
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1037_length_34199_cov_19.607750"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..99)
                     /locus_tag="PLI55_01620"
     tRNA            complement(10..99)
                     /locus_tag="PLI55_01620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(63..65),aa:Ser,seq:gct)
     gene            179..1990
                     /locus_tag="PLI55_01625"
     CDS             179..1990
                     /locus_tag="PLI55_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPA99568.1"
                     /translation="MNPLVISHRFFENVKKYADKPALLSKVDGHYRPVLYRDLGEIVK
                     AVALALDSWGIRKGDRVAILSENREEWVMSDMANQSMGAISVPIYPTLISNQIAPLFK
                     HSEPRVLFVANGTLLKKAMVTGYQFDRIVIYDPDPELAQAVAFAEIVETGRKLDAKNP
                     EAFSALLNAVEPDDIACINYTSGTTGAPKGALLTNKNFVKDVDNSVGIMKMVPEDCFL
                     SFLPLSHVLERMGGYYTPMLMGNTIGFAENIDTVVQNMAEVQPTILVSVPRLFEKIYA
                     TVMSQIESGSAIKKKVFFWALKAGKNRMHSYIDKTPLTFWQKRKIKIADKLVFNKLQK
                     TTGGKIQRFVSGGAPLSKEIAEFLFSAGLPVLEGYGLTETSPVVTLNGPDKVRLGSVG
                     VAIPETEFKIAEDNEILVRGPQVMKGYYKDEAATREVLDEEGFLHTGDVGYFDEDGFL
                     FITDRKKNILITAGGKNVAPQPIEDAIKLSPYISEAVLFGDRRSYIVALVTLDQEAVR
                     KWAENQHLSIDNYADFVRRPELITLIANEIERQTAHFSRFEKVKKFRIIPEDFTIESG
                     ELTPSLKVKKKAIAERYADLIDEMYQEQSQEKAQDLT"
     gene            2168..>4146
                     /locus_tag="PLI55_01630"
     CDS             2168..>4146
                     /locus_tag="PLI55_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99569.1"
                     /translation="MKKIIKISIIVIVSLVLLIGLTGFLILKTDSWKTILVSSVNRQL
                     MQNYNLKIEPEGIGGNIFYHLQFDRLKLTNQNQTELVTMSGVNLSYNLKSLIGHHHIL
                     NKLSLDTIKLAYPANIDSLLASFPEKKDTLASTGNFKLANILMSKCLIDDSHHPGQLL
                     VSIDSLNGSLSLNADTFNLQLNSTDFYVTGINEYFRIKQTAIAFVNNSLEIKNGQISN
                     RGTTVELDGRISLNKPYASQLRINVTDINPAERLPALSQVFRQDDIFDLSGEIKTSDK
                     TIKLDLGFSGTWHNQLLTNGRITGLIDGKQVNLAEMTFTKGMEQVHGTVVGNLDSSLT
                     AELRFSGIDASSWGLSAINTDLQGNLHVQTFGSIPNIDQVKVKIDLQESKIAPLDFNL
                     IRGELVYKDGLLTVLDSLYCVLGETKLIVEGEADLNGNSIDARAHFSDLNAEMLAPML
                     KMDTLSGVLDGFVEATGILTAPDLRGWIHGQQFGLPNLKFEESIARFGIVDVRTRKFG
                     DIFVEATNCQTNLIPEKIPLTSLIIHLNGDTTIVQTLKAIGENLDVEAKGYIVQFTDF
                     NLNSITILRSGNVLQNIDPISFSLKQDTINVDAVRLRLNEGILTLTGQVVRGELQFGR
                     LEFANLSIDPINTYLKGSQGVGGKLEGMVAYKKNL"
     assembly_gap    4147..4246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4247..5844
                     /locus_tag="PLI55_01635"
     CDS             <4247..5844
                     /locus_tag="PLI55_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPA99570.1"
                     /translation="PKMNCDLIIDDPVFDKLAGEKLTIKGVYQNERLSITEAMLVDQY
                     GHTRGRGYLALAISIVPARFEVLKEAPLSFNFTTNSSSPSFLTAYLDFLQEVQGDISL
                     ALGISGTLENPILSGNISVKGTTIYLDLLENPITGINGSAVIKDNILEIVSLTGHMKK
                     TPSSGDVGGFGSRLKSVTWDVLFPPKSDIDQPNVAINGTIDFTRFSAPKYNINLKGDE
                     LYIRTLLAEQEGLLSGNITMTGQDTVIYAGEIEVEDFVIRNEFTGTKKAVVPKQPSKI
                     YSIMNFHLIIPGSLEIKNSQLEGELEGDIWITRNGNEPFRFSGTLDILGGQFFAYGWE
                     FEIVRGSINFDPVEFNPTLDIEAQVDLASYGMSDTTTSSSNESEVVTVYLTGYLDNPN
                     IEFQSTNYSQSDILLFLSRAQSIGSITNGQDQLSASAANVANMWFERQLERNISRISG
                     LDDFELRTNGNLFSSQQPDQWSVMLGRKIAPNLYIKYERSFSLIEPYQQFGLEYRINR
                     NISIAGTYDQDGYSINYRYKYRY"
     gene            5892..>6925
                     /locus_tag="PLI55_01640"
     CDS             5892..>6925
                     /locus_tag="PLI55_01640"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPA99571.1"
                     /translation="MILIWSESLFSKNEFKATEYFVNKIIITGNKQFSTRKLKKQINL
                     KEKTIWRKQTFTRRLLELDRLNLESFYIKQGYLHCTVKDSFSIHSNNLVDVFFKINEG
                     QMYILKDVIISGAESLSEKKILRLLDHKINAPYNPIQIHNGIKNILNEYANTGRPLTT
                     ITDSLEINNDIRLHLIIHESEPFYINQVTIVNNKLVKEKPIRREIALQPGDLYSLKKI
                     ELSKRHIYETGLFSSVNIRLTDIDTVRNKVNLVVDVRELDMRYLGLDFGLGQDRGLRE
                     GREPYTSLEATGQWLNRNIGGRGSRLSLKTEGALNIDTESGLSQPRLSGEVTWIEPWL
                     LGFRSSNSFT"
     assembly_gap    6926..7025
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7026..7541
                     /locus_tag="PLI55_01645"
     CDS             <7026..7541
                     /locus_tag="PLI55_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPA99572.1"
                     /translation="LSGKIALVGGKPADYYKLEPSFSQYFNILGPVVLAYRVKVGIMA
                     PLRTGQPTPEYEKFYLGGSTSLRAWPDNKFEPDHIDPDGYNFKGLTSVEVRFPLFWLF
                     GGEVFVDGGNLAGEPDFSEFFKTYRWNYGFGLTLATPLGPIRIDYAIKIPKENEKKQP
                     GRFQLGIPYAF"
     gene            7554..10166
                     /gene="clpB"
                     /locus_tag="PLI55_01650"
     CDS             7554..10166
                     /gene="clpB"
                     /locus_tag="PLI55_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPA99573.1"
                     /translation="MYSSEKFTIKAQEIVQNAINTAQRLSNQAIEPEHFLYAILTTPD
                     NTGQLVLNKLIGNVEPFTRSVENLLETFPKVSGGSQSYLSNASNKMLQVAEDQAKQLQ
                     DEYLSAEHLLLGIIEVAKGELKNLLKKNGISNETILSALKEIRGSQTITDQFPEDKYQ
                     ALKKYCRDLNELARRGKLDPVIGREEEIRRVLQVLARRTKNNPVLIGEPGVGKTAIAE
                     GLALRIVSQDVPESFRDKRILALDMGALIAGAKFRGEFEDRLKAVLREISEAQGKIIL
                     FIDEIHTIVGAGAAEGAVDASNLLKPALARGDLRCIAATTFSEYRKYIEKDKALERRF
                     QPVLIDEPSIEDSISILRGLKERYENHHGIRITDAALVAAVELSARYITDRFLPDKAI
                     DLIDEAASRLRLEIDSLPAELDEVERKISQLEIESRALAKEKDIEESRQRYEKIQQEL
                     AQLKDEAAVLRARWQNEKKHITAISELKKQIEAKKLEAEKAEREGNWERAAQLKHYEL
                     VELNKQLEAEKEALKNVSGKRALLREEVTEEDIAEIVSKWTHIPVSRLVEGEREKLLK
                     MEEKLHERVIGQDEAIKAVSNAVRRARAGLQDENRPLASFIFTGSTGVGKTELAKALA
                     EFLFNDENALIRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEAVRRKPYSVILLD
                     EIEKAHSDVFNILLQVLEDGRLTDNKGNVANFKNSIIIMTSNLGSDLIMRQAGDVDEN
                     NIDVIYANIQETVMSQLRQILKPEFLNRIDEIIVFKPLLPKEIREVVRLQFNRLIERV
                     KTRGIQVKLSDEACDLIAKAGWDPAFGARPIKRIIQKQILDPLALEILEEKIQPGDTV
                     NIEIRDGKIHFVKG"
     gene            10277..10789
                     /locus_tag="PLI55_01655"
     CDS             10277..10789
                     /locus_tag="PLI55_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99574.1"
                     /translation="MRRILFSLLTTGMAVLLITCSKPDNTAFGYWDNAVALFNQSDYQ
                     GCIKQYQKIIKYFPDDSLAVPAMFAMADVYKNNLNDADQAIKIYKEIIRKYGDSERAA
                     NAHFMIGYVYANEIRDLKKAEEYYKSFLKKYPTNILAPSAEWELKNLGKTLDEIPELQ
                     KITQEKQTAE"
     gene            10794..11786
                     /locus_tag="PLI55_01660"
     CDS             10794..11786
                     /locus_tag="PLI55_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPA99575.1"
                     /translation="MTFNLAEMNERIQRESAFVSKIKSEMAKVIVGQEQMIERMLIGL
                     LCNGHILLEGVPGLAKSLAVSTLAKIIQVNFRRIQFTPDLLPADLVGTMIYNQKTGTF
                     ETKRGPIFSNIILADEINRSPAKVQSALLEAMQERQVTIGETSHPLDDPFMVLATQNP
                     IEQEGTYPLPEAQVDRFMLKVIVDYPQKDEELDIVKRMARNAAIPAVNPVITAEDIRR
                     ARQTVDDIYIDEKIHKYIVDLVFATRDPKAVNLADIADLIQYGASPRASIFLAMAGKA
                     QAFIAGRGYVTPEDIRTIIWDVMRHRIILTFEAEAEEVTVEEIIRRIIETVAVP"
     gene            11813..12691
                     /locus_tag="PLI55_01665"
     CDS             11813..12691
                     /locus_tag="PLI55_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPA99576.1"
                     /translation="MLSKEILKQVRQIEIRTRHLVNDVFSGEYHSVFKGRGMEFSEVR
                     EYQYGDDFRSIDWNVTARYAHPYVKIQEEERELNVMLAVDASRSNAFGSQGKFKNEIA
                     AEICALLAFSAIKNNDKVGLLIFTDQVEKFIPPHKGRSHTLRVIREILYFQPQSPRTN
                     IKSGLEFLLKGLKRRAIVFLVSDFNDENFAKPLRVLNKKHDVIAIHLQDARDKILPPV
                     GLLNLKDAETGDEVWVDTSDRAFLEQYADLVEQQRQNLRNMARGMKLDLIEIDTAVSY
                     VDPLVKFFKMREQRFR"
     gene            12711..13667
                     /locus_tag="PLI55_01670"
     CDS             12711..13667
                     /locus_tag="PLI55_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99577.1"
                     /translation="MQLRRSKFNFLALFSFIFILFQLTSAQNSGIIIQTTIDTTIATI
                     GDHLHLRINLRYPIGTIFELPLVEETIGQLEVIDNQLSPPRKKGQINEQEWQLTLAVF
                     DTGQITIPALEIKAHPANDTASVLTFQTDSKTVQVYSVLPPDKRELKDIKPPFPLRRS
                     ISGKAILLILLLLLIVGGIWYYRRWEKAHPPLIIDEKFLEPPHIVTLNQLNALKEKMP
                     TTPDEMRAYYFRISEILREFLERRFFIRALEMTTAEIAEAFKYLDIEPALTREWIELF
                     SELDIVKYAGQTPAEKDIAAVLEKACHCVESTKYETFLRRTM"
     gene            13664..14611
                     /locus_tag="PLI55_01675"
     CDS             13664..14611
                     /locus_tag="PLI55_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPA99578.1"
                     /translation="MIEVQNLVKHYGSVKAVDNISFTVEKGEILGFLGPNAAGKTTTM
                     RIITGYMPASAGTVKVAGYDVFEQGLEVKKRIGYLPENPPLYSDMRVREYLTFVARIK
                     GVNPKTINAEVDRVAERLSVLEVMDNLISNLSKGYKQRVGLAQALLNNPPVLIFDEPT
                     NGLDPRQIIEVREIIKSLGSDHTVILSTHILPEVSMTCNRVAIINEGKLVKIDTPENL
                     TKQLRGSESIILQVEGPMNEIEIALSQHQSVTAVAVQPTGKENIATYRVETSIGSDIR
                     KELASLVVNRGWGLLELKAESLSLEDIFLKLTTKEEEVN"
     gene            14613..15380
                     /locus_tag="PLI55_01680"
     CDS             14613..15380
                     /locus_tag="PLI55_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPA99579.1"
                     /translation="MSNLLAIYKRDLKGYFFSPIAYVVIGLFLLVNGIFFYVLLSSFL
                     EYSYAVMIQRQGYPINVNLLMIRPFFQNMSVIVLFVIPMITMRSFSEEKKSGTMELLL
                     TSPVTNWQLILGKFLASFTLYFVMVMVTFLFMVFLLIWGNPSLVPILISYLGILLMGL
                     VLVALGNFISSLTENQIVAAVGTFGATMFLWVIGFAGDFAGKVFGEIFKYLSIVNHFD
                     DFSKGVFDTNHLVYYLSLTFLMLFFTYQSIESSKWRG"
     gene            15384..16901
                     /locus_tag="PLI55_01685"
     CDS             15384..16901
                     /locus_tag="PLI55_01685"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HPA99580.1"
                     /translation="MKIYKWVFSIGLILIACGLIYFSIVNIWSWPVIVLLILGFGASV
                     FALIKLDIRTLLKNRRLIYGGNMVLVIILIIAIVGLVDFFFTRHSWRVDTTATKIFSL
                     SDQTRKILKNLDKDIEIVVFAKKSNKSYINDRLNEYRHYSKRISWEVVDPDEKPALAK
                     QYNVKNYGNLVIICEDKAELVPNFSEEAIINAIIKVTREGKKKVAFLTGHGEGTIAST
                     ERDGFERAKQAIEQQNYAVEELMLVDKDSIPADISVLVIAGPQKPLFEKELDLLTKFI
                     ENGGSSLIMLDPRPAPGLSDYMKGWKIEVGDNLIIDASGFGRLFGAGPDIPLVTDYTD
                     HPIVKDMKNVMTFFPMARTVRPLESDNKENIIVEELIRTGQQSFAVANIEEVYRTGRV
                     SRGPNDLQGPLSIACAATIENPSGQREGRLVVVGDSDFASNAYFDNQANGDLFMNMIS
                     WLIADEDLIAIRPKNPEISTVNMTPAQTKTVMWLTVIILPAIAFGVAIMVYVRRK"
     gene            16905..18275
                     /locus_tag="PLI55_01690"
     CDS             16905..18275
                     /locus_tag="PLI55_01690"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPA99581.1"
                     /translation="MKKGRSTLILFGIFVVLFAAVYFFEIKGGEKRAKTSKLASQLFQ
                     IEKDNIKQLILSGDYGEITCEKDGAEWIITAPVLAQGDAQSIESNLDLILGTSIERKI
                     ADSTADLSLFGLLEPRGKVQVVTQSGKHTIVLIGDENPTGDLIFVKYPDDNAIYTTNK
                     SLWTYANKKLYDLRDKKIMHFNLDEVRKINITSRAKGKVSLEKVAGKWQIVDPVNLPA
                     NDSEVKSLLTRLSTGRAKEFIDEEPDDLKKYGLTNPAVMITLEIGESLARTSFLVGDT
                     ATNDGGGFYAKEETRRPVFTVEKWTVDGITKSAFDLQDKKLLGFDNQKCDRIVWQIAN
                     QEYIATKIDSVNWMIVSPETLQVDENLMRRWLEALRDFSVDELESYQPKSLSVYGLDK
                     PTLRLAFYHKDSKSDELLVGKEISEKYYVKIGNYPHVYRIKKNTFERIYKKPEDLMIK
                     PVSTES"
     gene            complement(18353..19267)
                     /locus_tag="PLI55_01695"
     CDS             complement(18353..19267)
                     /locus_tag="PLI55_01695"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010933874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPA99582.1"
                     /translation="MNYYNYNQSRYKSGYIRPRFSRGAVGNIIIANVVIYLIMVIFQA
                     QNFFAFNFGLVPYAVLKKGYVWQLFTYMFIHGGFGHIFWNMFVLWMFGMEIENYWGKR
                     EFYKFYFITGVGSGLITMLFSAHSDIPVVGASGAIYGVLVAFAMLFPERYIYFYFLIP
                     IKAKYFAIIMVVITFFSTLSPGTGNISHLTHLGGLVIGYLYLARYKLLSGWHRRTGSA
                     NWHWPRPKIKNPFRNLHLNLQSKKSKKSRDREQAPLSYETEETMREELDHILDKISTD
                     GYDHLSEQERSTLYLLSKYFAEKDRRKS"
     gene            complement(19271..19972)
                     /gene="recO"
                     /locus_tag="PLI55_01700"
     CDS             complement(19271..19972)
                     /gene="recO"
                     /locus_tag="PLI55_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPA99583.1"
                     /translation="MAIIRTRAFVIKTIPFKDTSLIARLFTESNGKVAVLAKGARNLK
                     SPFRGYLEPLSLLEVQFYYKPTRDIQTLSQAETIRSFLSNSTEIEPTIMATAILECIE
                     KFIREHHEDVPIFKLVLDTLNYIEAQNDNSQLAFVYFILNLTTLMGYEIGLNSSRNSD
                     WSTLPFSMEELEILKKIEKADIRTGLPLCNLNLPIMAIAKTLVNYLAMQTDLPVRLKS
                     FELLSQIIQSADRKD"
     gene            complement(20185..21630)
                     /gene="proS"
                     /locus_tag="PLI55_01705"
     CDS             complement(20185..21630)
                     /gene="proS"
                     /locus_tag="PLI55_01705"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPA99584.1"
                     /translation="MSISVTKRSEDYAKWYTDVVLKAEMADYGPVKGTMVIRPYGYAI
                     WENIQSIADRMFKETGHVNAYFPMFIPESYMKREAEHVEGFAPECAIVTIGGGKPLEE
                     PLYVRPTSETIIWASYKKWIRSYRDLPILINQWANIVRWEMRTRLFLRTTEFLWQEGH
                     TAHATPEEAEEEARRILEIYRQIAEDYLAMPVLVGRKTEAQKFAGAVHTYAIEAMMGD
                     KKALQAGTSHNLGQNFAKAFDVTFLNKSNQLEYVYATSWGISTRLIGGLIMTHGDDKG
                     LILPPRVAPYQVVIVPIWKSEEEKCAINEFVANLTTRLKAEQIRFRYDNSEQETPGWK
                     FNYYEMKGVPIRLEVGPKDVAKNQVVLVRRDPTEMEPIGRKQPIPVDNAPTVIKNLLE
                     EIQTNLFKRAQAFRENNTFQAENYDQFKELIEAGGFIRVFWAGNSADEARIQEETKAT
                     IRVIPFEQKEKGTCFYTGKTTDRIVIFARAY"
     gene            complement(21632..22318)
                     /locus_tag="PLI55_01710"
     CDS             complement(21632..22318)
                     /locus_tag="PLI55_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HPA99585.1"
                     /translation="MFDEFLKIWRKKSLTAETVDQALDMLTKTREMFVESMWSLRESD
                     TGELRLDIYQHDKTINKLVRDIRKKLMTHLMISDKSEVISGLTLAIVVNNIERIGDYT
                     KNIADLAILHKKTLHAAEFEETVVSIEKRVKDFFDWTIKAFPESDVELARQVVHQYKD
                     VSRDTTILINTLIKGECEFNISDAVTLALYIRFLKRVGAHLYNICTSIVNPFHRIGFK
                     EKNNEINQGN"
     gene            complement(22322..23416)
                     /locus_tag="PLI55_01715"
     CDS             complement(22322..23416)
                     /locus_tag="PLI55_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HPA99586.1"
                     /translation="MNFRDRKYWVGFAKALAIIMLIYLFLLSVTLIGDTFKMMGQGFA
                     QKIMNGVSNPFVGLFAGMLCTAIVQSSSLTTSLVVSMVAGNVLTINLAIPIIMGANIG
                     TTITNVLVALGHIGKKDEFERAFAVATMHDFFNILSVIIIFPFQYFFNILGIAAGAVA
                     TAFENIGALKFINPISKILEPVSQFIIDLFPNYPWINIVLALVILFFALTFLVKVIKS
                     LVLAKVELFFDQYIFKSANRSFLLGLLLTAIVQSSSITTSLTVPLAAAGVLSQKKVFP
                     YAVGANIGTTITAFLAAIATNSVDAITIAFAHTLFNVIGACIFLPLQKIPIELARLLA
                     SLTRKNRLYAIAYVILLFFIIPLIIIFITR"
     gene            23736..25082
                     /locus_tag="PLI55_01720"
     CDS             23736..25082
                     /locus_tag="PLI55_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPA99587.1"
                     /translation="MKSSNIFDVILIGAGPAGLFAAETLAGKSLSVLVLDRGDEPLKR
                     HDVNFGIGGAGAFSDGKLNLTSRIGGEPESFGRKADEVEELIRQIDDIFTSLGVIGGY
                     SGEDGEAYQALLRSAHAAGVEFIFAKQRHIGTDRLQDIIQRFYERLIEKGINFRPNTY
                     VDKISKEGDYFKLICKDEVLFARAVIAAPGRAGAYWLREQAHNLGIHTMYGPIDVGVR
                     VEFPDDIYRPIAAVMYDAKFRLYSKSYDDLVRTFCTNPSGYIVTEKYDDFVLVNGHAK
                     WNSKTDNTNFALLSRVVLTDPVEDTTKYGRDIARLATTIGGGKPIIQRLTDFVSGRRS
                     NWERISRSATVPTLKDVTPGDIAMAFPHRIVTNLIEGLTVLNKIISGLMSNNTLLYAP
                     EIKFYDTKYKVTPYLETTLENFFVAGDASGHSRGIVYSAVTGIFAAQGVLKNLGKD"
     gene            25141..25617
                     /locus_tag="PLI55_01725"
     CDS             25141..25617
                     /locus_tag="PLI55_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HPA99588.1"
                     /translation="MKSPENKDKFMREGLEYIKQIVNRRGSRDTKLLEICRFLNDKFI
                     GFDWVGFYFSDNLKKELILGPFIGDPTEHRNIPFGRGVCGRAAVSGTTVNVADVTAES
                     NYLACSLEVRSEIVIPIFKSGQFVAELDIDSHQSDRFGNSERQFLEQVGQIISVIF"
     gene            25682..27313
                     /locus_tag="PLI55_01730"
     CDS             25682..27313
                     /locus_tag="PLI55_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPA99589.1"
                     /translation="MIYKRINYVRLRQIMRKATIPVIIIIVVLGFLFISPFGKSIIAS
                     GSDLYQKLNIFQDIIRIINDSYVEEPDWNKVMEGAYRGMLEELDPHSVYIEPKQLDDL
                     NEQFSGKFEGIGIEFDILDGYITVISPVAGAPAERAGLEVGDKIVKIDGESAYRISRD
                     DVQKKLRGPKGSEVTVTVRRQGQTDFDVKIIRDEIPLYSVSAAFMIDDSTGYIFLNRF
                     SGTTSDEVEKALNRLAAEGMQRLVFDLRNNSGGYLEQAVEIADKFIAGRQKIVYTRGR
                     ISSANEEFYSSKTGPYEKIPLVILINRGSASASEIVAGAVQDLDRGLIVGETSFGKGL
                     VQRQYPLRDGSAIRVTVARYYTPSGRLIQRPYDGAIDDYYSTFNEENRDSLLAIEDSV
                     EKRPEFSTIGGRTVYGGGGITPDYSVKYDQALNNETYKLLRHPSRVIFEYASDFVNKN
                     RDLAADRKNFYKNFEVSNKDFEAFKKMAVEKIGDLNIKQVEKDAEYLKILIKAEIAHG
                     YWGYDEYYKIVRLSDNQVTSAVDFLNEARKMLQIQ"
     gene            27414..28037
                     /locus_tag="PLI55_01735"
     CDS             27414..28037
                     /locus_tag="PLI55_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPA99590.1"
                     /translation="MVELFLRGGIFMWPILGLAIFGIMVVIERAYTLSKASRITKRFL
                     PKVLNTLKEEDGVKKAIELCENTPGPIAEIFHAGLSAVGNGMEAVEKAIQSAGTVEMA
                     FLDKGMIWLATVITLAPLLGFMGTVSGMINAFDAIAKVNDMSPAIVAGGISEALLTTL
                     FGLIVAAPLQLCYNLFVARVDSIVVEMEESTMNLLQTLDVINKENCK"
     gene            28040..28438
                     /locus_tag="PLI55_01740"
     CDS             28040..28438
                     /locus_tag="PLI55_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPA99591.1"
                     /translation="MALLKKKKTRKVDIPSASQADIAFLLLLFFICTTTVDVDKGIGL
                     ILPPEGDSPVEISKKNITTIVIDPQGNVLIDNKEVEINQIRDIAERLQMENPKMIFSV
                     KAHPRCRYDKYVAVLDQLKMAKATRISIAE"
     gene            28450..28857
                     /locus_tag="PLI55_01745"
     CDS             28450..28857
                     /locus_tag="PLI55_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPA99592.1"
                     /translation="MAFQHKKNISTEVPTASMPDIIFTLTLFFMATTVMRQYSGLPVV
                     LPKAKQIEKLQSKIHVTELWVSKEELISIDGKLVNLQDIRNIMYNKRVRDPQLIVSIR
                     ADERVTMDLMSKIHKELRLADALKVNYSTKTAS"
     gene            28868..29539
                     /locus_tag="PLI55_01750"
     CDS             28868..29539
                     /locus_tag="PLI55_01750"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPA99593.1"
                     /translation="MILRKKPEASLKLRQPLMYRVGLVVALVLITGIMIAFPKFEERE
                     VVQRKVDVILEVTDIPQIEQKIEAAKPPSRPSIPIESENEDLSEDITIDQTTDFDSYT
                     AWEAPPPPPEQHSDEGEGPRVRFIPYDEPPEPIGGFAEIQKNIVYPEIAQEAGIEGTV
                     VVQAYVNEYGVVTECTILKGMPNTGLEEAAIAAIKKTRFKPAKQRDRNVGVYISIPVI
                     FKLKS"
     gene            29802..30470
                     /locus_tag="PLI55_01755"
     CDS             29802..30470
                     /locus_tag="PLI55_01755"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA99594.1"
                     /translation="MNLSNPTENRPIYAKTWQNVAAVERYALSRYRHYDQRLISWREK
                     RIVNHLLKQIKNGNHILLDIPSGYGRFTPLFRQHGFKVINGDLNLYALLYQRQRFPDS
                     NLMFVTDGNCLPLPDNSAGVVFNFRLLQHFQTSDQRIALLRELARVTNCYLIVSVYLE
                     SNFHRLAQLVSRRKRRMTMISRTQWDTELRSCGLKILRISRPLRFLHAQNIFLLEKSP
                     IIEK"
     gene            30498..32537
                     /locus_tag="PLI55_01760"
     CDS             30498..32537
                     /locus_tag="PLI55_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPA99595.1"
                     /translation="MKKLITIIFTVIVLATSAKPQDLLTVFTEPYLSGSVADNVTWSP
                     NDSLIAFLWNDQGGEFRNLWIANPQTGAKEQLTDFNPTTNVNLPTIGTLGWLNNRCLF
                     FEYQGDIWEASLLPGYPLKRIEGLTNCKPPIALAPNRRYLTYNRGGYLRLFDFITKEE
                     QTIIPDKAYQRPPARQVCAYQTTYCWSNDSKKVAVYLESDADENTVHFKVFDVLKGST
                     DDIVIKERPNDNSIVRNFIWSPDNQSIVIESVTSNLRERYILLVDLTKDRLDTLYREI
                     SNNWIADFGYQLYWIESEAKILFGMEYNSYNHLFLLDPTNGHYSALTRGKWDVKDYAV
                     DATGQLVYFTATKDNPEQLQLYSAQIKTGEITDISYRGGDYSFQLSNNSRWIAEVFSN
                     YSTPPDLYWIEASPQSKMNQITDRPDSPLEKPLRKIPKSGTIQNAITGKPVNYRVWLP
                     DDFRVNHKYPIIVVLNGADCLAPLTEAWRPECYFKQWLSDKGYIVVAVQYTDLRTAIE
                     PADSESFSDPLKIQLADIQAVVKEISKLQYVDLSRSGICGWGYGGYLATMAMFETDNI
                     FRAGVAILNDFSWSQSSSIYLNYMANLLANDQVFRKIDPHNFCYNLRGRLLVIQSASS
                     TLPQLVDAQRLIPELLSIRRPVDFYLYPWENNPIMRSANQYDMFIKTARFFQLNL"
     gene            complement(32561..32884)
                     /locus_tag="PLI55_01765"
     CDS             complement(32561..32884)
                     /locus_tag="PLI55_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPA99596.1"
                     /translation="MNSTKPVAQIKIELPESKAEGNYSNFILISHSNAEFVLDFARIM
                     PGSPKAVVQSRIILTPIHAKTLLFTLQDNLAKFEAKFGEIKLPDKESFEPFGFPNSEE
                     SKLPN"
     gene            complement(32914..33330)
                     /locus_tag="PLI55_01770"
     CDS             complement(32914..33330)
                     /locus_tag="PLI55_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99597.1"
                     /translation="MEDLVCPECNAPLKEEDLKQSLVCPNCKTNLRDSKYLDFLELLV
                     YYDIIDDIDFFDMSLYGKEMLEHEREDFDEEEIDPSKFEKKKEVWDEFEDDLELNENL
                     TEEIESSDDAWDLSDTDIPFEDEWDEDNEDREEDEY"
     gene            complement(33383..33952)
                     /locus_tag="PLI55_01775"
     CDS             complement(33383..33952)
                     /locus_tag="PLI55_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99598.1"
                     /translation="MNCTNYQQFNVLNHKAIHKIFSWPLDTSLQESESTKFARVLICL
                     PFEHAHFYSALKVFLPFIRQKPELVRLLVPQAFFTLIQEINPDLLIPLISSRINKEGF
                     PTDPEVLALARINLEFAIDLNVVPTVPTSFIVSRCRAPIKVGFQSELAEKLFNLIINY
                     KTDRLVEKSYNLIWDLLQPQIQKVNKNLT"
BASE COUNT         9927 a         6778 c         7486 g         9808 t
ORIGIN      
        1 aaaatcttgc ggagagacag ggattcgaac cctgggtacc gcttttggca gtacaactgc
       61 ttagcaggca gctgccttca gccgctcggc catctctcct ctaaaaatcg cgataataaa
      121 aactggctga atttattgaa ttaattcttt aaattcaaat tacattttgg aggtaaatat
      181 gaatccttta gtaatttcgc acaggttttt tgagaatgta aagaagtatg cggataagcc
      241 ggcgctttta tccaaagttg atggtcatta ccgaccggtt ttatatcggg atttaggaga
      301 aattgtcaaa gcggtggcgt tagctctcga ttcgtggggt attcgcaagg gcgatcgcgt
      361 ggcgatattg tccgagaacc gcgaagaatg ggttatgtcc gatatggcta atcaatccat
      421 gggagctatt tccgtaccaa tctacccaac cttgattagt aatcagatag caccgctatt
      481 caaacattcc gagccgcgag ttttgttcgt ggctaatgga actttactga aaaaagctat
      541 ggtcactggt tatcaatttg atcgtattgt gatttatgat cccgatcctg aattggcgca
      601 agcagtggct tttgcggaaa tagtagaaac aggacgtaag ttggatgcca agaatcccga
      661 ggcttttagc gctcttctca atgcggttga gcccgatgat atcgcttgta taaattacac
      721 atccggcact accggcgccc ccaaaggcgc acttttaacc aataaaaatt ttgtcaagga
      781 tgttgacaat agtgttggta taatgaaaat ggtgccggaa gactgcttcc tgtcattttt
      841 gccgcttagc cacgtattgg aaaggatggg aggatactac acgcctatgc ttatgggcaa
      901 cacaatcggc tttgccgaga atattgatac ggtagttcaa aatatggctg aagttcagcc
      961 gacaatcttg gtcagtgttc cccgtctgtt tgaaaagatt tacgcgacgg tgatgtcaca
     1021 aatagaatcc ggctcagcca taaagaaaaa ggtctttttt tgggcactga aagctggtaa
     1081 aaaccgaatg cattcctata tcgacaaaac accgttgact ttctggcaaa aaaggaaaat
     1141 caaaatcgcc gataagctgg tttttaacaa actgcagaaa acaaccggtg gcaaaatcca
     1201 gcggtttgtc tccggtggcg caccgctttc taaggaaatc gctgagtttt tattctcagc
     1261 cggtttaccg gttctggaag gttacggttt aaccgaaacc agcccggtgg taactctgaa
     1321 tggtcctgat aaagtccgtc tgggttcggt cggagtcgca attccggaaa ccgaatttaa
     1381 aattgcggaa gataatgaaa ttctcgttcg cggaccacaa gtaatgaagg gctattacaa
     1441 agacgaagcg gctactcggg aagttttaga cgaagaaggc tttttacaca ccggcgatgt
     1501 aggttatttc gatgaggatg gatttttatt catcaccgat cgtaaaaaga atatcttgat
     1561 cactgccggt gggaagaacg ttgcgcctca gccaattgag gatgcgatta aattaagtcc
     1621 ctacatttcc gaagcggttt tgtttggcga tcggcgcagt tatattgtcg ctttggtgac
     1681 cctcgatcaa gaagccgttc gaaaatgggc cgaaaatcaa catctttcaa ttgacaatta
     1741 tgctgatttt gttcgtcggc cggaattgat caccctgatt gctaacgaaa tagaaagaca
     1801 aacagctcac ttttcccgct ttgaaaaagt gaaaaagttt cgcattattc ctgaggattt
     1861 cacgatcgaa tccggtgaat taactccttc cttgaaagtc aagaaaaagg ccattgctga
     1921 aaggtatgct gatctgattg atgagatgta tcaggagcaa agtcaggaaa aagcccagga
     1981 tttgacataa ttttcggtta ggcaacttta ttcagctaat ttttccactt actgtcaatt
     2041 gccggtaaat cctattcttt taatggaaca accggcaatg tgactgatta aatagtcttg
     2101 tgtacggttc tgacgatgtt ggagaattaa gaacgcgtgg ataaatttgc ccggtgttga
     2161 ggtttttttg aagaaaataa tcaaaatatc aataatagta atagtttcgc tggtcttatt
     2221 gatcggcctg actgggtttt taattctaaa aaccgattcc tggaaaacaa ttttagtcag
     2281 ttctgtcaat cggcaattaa tgcagaacta taatctgaaa attgagcccg agggaatcgg
     2341 tggcaatatt ttctatcatc tacaatttga ccgattaaaa ctgaccaatc aaaatcaaac
     2401 cgagctggtt acgatgtcgg gtgtcaattt gtcatataat ttaaaatcgt taatcggtca
     2461 tcatcatata ttgaataaat tgagtcttga cactatcaag ttggcttatc ctgcaaacat
     2521 tgattcttta ttagccagct tcccagagaa aaaggatacc ctagcgtcaa ccggtaattt
     2581 taagctggca aatatcctga tgagtaaatg tttgattgac gacagtcacc atcccgggca
     2641 attattagta tccattgatt cccttaacgg ctcgctgtcc ctgaatgctg atacttttaa
     2701 tctccagctc aattcgactg atttttatgt gacaggaata aacgaatatt ttaggattaa
     2761 gcagaccgca atagcgtttg tcaacaattc tctggaaatt aaaaatggtc aaatcagcaa
     2821 tcgcggaacg actgtggaat tggacggtcg aatatcgttg aataaacctt acgccagcca
     2881 gttaagaatc aatgttactg acattaatcc ggctgaacga ttaccggctc tcagtcaggt
     2941 attccgccaa gatgatattt tcgacctgag cggagagatt aaaaccagtg ataaaacaat
     3001 caaactggat ttgggatttt ctggaacttg gcataatcaa ttattgacga acggccgaat
     3061 tactggtttg attgatggca aacaggtcaa tttggcggaa atgactttta ccaaagggat
     3121 ggagcaggtt cacggcaccg ttgttggtaa tctcgattca agcttaaccg ccgaattgcg
     3181 ttttagcgga atagacgcca gctcgtgggg gctatcggcg ataaatactg atttgcaagg
     3241 caatttacac gtacagactt ttggttctat tccgaatata gaccaggtca aagtaaaaat
     3301 agatttacaa gaaagtaaaa tcgcaccctt ggatttcaat ctgattcgag gagagttagt
     3361 ctataaagat ggattattga ccgttttaga ttcgttatac tgtgtgcttg gtgaaactaa
     3421 attgatagtc gaaggcgaag ctgatttgaa tggtaatagt attgatgctc gtgcccattt
     3481 ttcagattta aatgctgaaa tgttagctcc tatgctaaaa atggatacat tgagcggagt
     3541 tctcgatggt tttgtcgaag ctactggaat tttgactgca ccggacttac gaggttggat
     3601 tcacggtcag cagtttgggc tcccgaactt gaaatttgaa gaaagcatag cccggtttgg
     3661 cattgtagat gttcgtacca ggaaatttgg tgatattttt gttgaagcga ccaactgtca
     3721 gaccaattta atcccggaga aaataccttt aacttcgcta attattcatt taaatggtga
     3781 taccactata gttcagactt taaaagccat cggtgaaaat ctggatgtag aagctaaagg
     3841 ctatatcgtc caattcacgg atttcaattt aaatagtatt actatcttgc gtagtggcaa
     3901 tgttctccaa aatattgatc cgatttcgtt ttcgttaaaa caggatacga tcaacgtgga
     3961 tgcggtcaga ttgcgtttga atgaagggat tttgacactt actgggcagg tggtacgagg
     4021 cgaattacag ttcggccgac tggaatttgc taatctctcc attgatccga tcaataccta
     4081 tttaaaaggt tcacagggcg tcggtggtaa gctcgaaggc atggtggctt ataaaaagaa
     4141 cctggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaccc taaaatgaac
     4261 tgcgatttga ttattgacga tccggttttt gacaaactgg ccggggagaa gctcactatt
     4321 aaaggtgttt accagaatga gcgtctttca atcaccgaag ctatgctggt agatcaatat
     4381 ggtcatactc ggggcagagg gtatttagcg ctagcaatta gtattgtgcc tgcccgtttt
     4441 gaggttttaa aggaagcgcc gctgagtttt aatttcacta cgaacagttc atcaccatca
     4501 tttctaactg cctatcttga ttttctgcag gaagtccagg gtgatataag tctcgcgctg
     4561 ggcatttcgg ggacactgga aaatcccatc ctatccggaa atatttccgt taagggcacc
     4621 actatttatc tcgacctgct cgaaaatcct attactggaa ttaacggcag tgcggtgata
     4681 aaagataata ttcttgaaat tgtttctctg actggtcata tgaaaaaaac tccttcatcg
     4741 ggcgacgtag gtggttttgg aagccgccta aaatctgtaa cctgggatgt gctatttcca
     4801 cctaaaagtg atattgacca acccaatgtc gcaattaatg gtacaattga ctttacccgg
     4861 ttttctgcgc ccaagtataa tattaatcta aaaggcgatg aactatacat ccgtacttta
     4921 ctagcggagc aggaaggctt gcttagcgga aatattacta tgaccggtca ggacacggtt
     4981 atttacgcgg gagaaattga agttgaagat ttcgttatcc gcaacgaatt tactggtacg
     5041 aaaaaagcgg ttgtgcccaa acaaccctca aaaatatatt caattatgaa ttttcatttg
     5101 atcattcccg gcagtcttga aattaaaaac tcacaattgg aaggagagct cgagggcgac
     5161 atctggatta cacggaatgg aaatgaaccg ttccgttttt cagggacgct cgatattctc
     5221 ggcggacaat ttttcgctta tggctgggaa tttgagatag taagaggctc gatcaatttc
     5281 gatccggttg agtttaatcc aactctggac attgaagccc aagttgacct agcttcctat
     5341 ggtatgtcag acacaaccac atcctcttct aacgagagtg aagtggtaac agtatattta
     5401 accggctatt tagataatcc taacatcgaa tttcaatcaa ctaattattc ccaaagcgat
     5461 attctgctct ttttatctcg ggctcaaagt atcggctcaa taaccaatgg acaggatcaa
     5521 ctttcggcta gtgccgcaaa tgtcgccaat atgtggtttg agcgtcaatt ggaacgtaat
     5581 atcagccgga ttagtggact ggatgatttt gaattgcgta ccaatggcaa tctcttttcg
     5641 agtcagcaac ctgaccaatg gagcgtaatg ttgggacgta aaatcgctcc caatttgtat
     5701 ataaaatatg aacgctcttt ttctctaatt gaaccctatc aacaattcgg actggagtat
     5761 cgaattaacc ggaacatctc gattgctggg acttatgatc aggatggata tagtataaat
     5821 taccgttata agtatcgcta ttgaaaatca taaacagaaa ttttcggtca tacagtttaa
     5881 ttttaaccat tttgatttta atatggagtg agtcgctttt ctctaaaaat gaatttaaag
     5941 caaccgaata ttttgtcaac aaaattataa taacgggcaa taaacaattt tcaactcgca
     6001 aactaaaaaa acaaattaat ctcaaagaaa aaactatctg gcgcaagcaa acctttactc
     6061 ggcgactgct ggagcttgac cgccttaacc tcgaatcatt ttatattaaa caaggttatc
     6121 ttcactgcac ggttaaagat tcattttcaa tccactccaa taatctcgtt gacgtttttt
     6181 ttaagatcaa tgaaggtcag atgtatatcc tgaaagatgt aatcattagc ggagctgaat
     6241 cccttagcga gaaaaaaatt ctcagattac tcgatcacaa aattaatgca ccatataacc
     6301 caattcagat ccacaatggc ataaaaaaca tcctgaacga atacgccaat accggccggc
     6361 ctttgacgac gattacagat tcgttagaaa taaataatga tattcgacta catctgatta
     6421 ttcatgagtc cgaacctttt tatatcaacc aagtaactat tgtcaataat aaattagtca
     6481 aagaaaaacc tatccgccgg gaaatagcct tacaaccagg tgatctttac tccttgaaga
     6541 aaatagaact gtcaaaaagg catatttatg aaactgggct tttttcgagt gtcaatattc
     6601 gcctgactga tattgacacg gtccgcaaca aagttaatct tgtagttgat gtccgggagc
     6661 tggatatgcg ttatcttggt ttggattttg gtcttggcca ggatcgtggt ctgagggaag
     6721 gtcgcgaacc ttacacttct ctagaagcca ccggacaatg gttgaatcgt aatattggcg
     6781 ggcgcggtag tcgcctcagc ctaaagacgg agggagcgct taatatagat accgaatcag
     6841 gactttctca gcctcgcctt tcaggagaag ttacctggat tgaaccgtgg ctattaggat
     6901 tccgctcctc taactctttt actttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnttaag cggcaaaata gctctggttg gtggcaagcc cgccgattac tataaattgg
     7081 agccatcttt cagtcaatat ttcaatatct tgggaccggt cgttctggca tatcgggtaa
     7141 aagtcggtat tatggcgcct ttgagaaccg gacagcctac tcctgagtac gaaaaatttt
     7201 atcttggcgg ctctaccagt ttacgcgctt ggcctgataa taaatttgaa cctgaccata
     7261 ttgatcctga tggctataat tttaagggct taaccagcgt tgaagtccgc tttccgctgt
     7321 tttggctttt tggcggagaa gtttttgttg atggtggcaa cttagcaggc gaacctgact
     7381 tttctgaatt ttttaagaca taccgttgga attatggatt tggcctaact cttgctactc
     7441 ctttagggcc aataaggatt gattatgcaa tcaagatccc taaagagaat gagaaaaaac
     7501 aaccggggag attccaatta ggaattccct atgcattcta aggaggatgt aaaatgtatt
     7561 caagtgaaaa atttaccatc aaagctcaag aaatcgtcca aaatgccatc aatactgctc
     7621 agcgactttc caatcaagca attgaaccgg aacatttcct gtatgcaatt ctgactactc
     7681 cagacaacac tggtcaatta gttctgaaca aattgatcgg gaacgttgaa cctttcactc
     7741 gtagtgttga aaatcttctg gaaacttttc ctaaagtcag tggtggaagc caatcttatt
     7801 taagcaatgc ttccaataaa atgttgcagg tagctgaaga ccaggctaag caactccagg
     7861 atgaatatct tagcgccgaa catcttttat taggcataat agaagttgct aaaggtgagt
     7921 taaaaaattt attgaaaaaa aatggtattt cgaacgaaac cattctgtca gctttaaaag
     7981 aaatccgtgg ttctcaaact atcaccgatc aatttcctga ggataagtat caggcattga
     8041 aaaaatactg ccgggatctc aacgaattag cccggcgcgg gaaactcgat ccggttatcg
     8101 gacgtgagga agaaatccga cgagttttgc aggttttagc gcgccgcacg aaaaataatc
     8161 cggtgcttat cggcgaaccc ggcgtcggca aaacggctat cgccgaagga ttagccttgc
     8221 gcattgtttc tcaagatgtg ccggagagtt tccgagataa acgtatttta gcgctggata
     8281 tgggagcttt gattgccgga gccaaattcc gcggtgaatt cgaggatcgc ttaaaagcag
     8341 tcctgcgtga aatttccgaa gctcagggaa agatcattct gtttattgat gaaattcata
     8401 cgatcgtggg agccggtgcg gctgaagggg ctgttgatgc ctccaaccta ttgaaaccag
     8461 ctctggcgcg tggtgatctg cgttgtattg cggctaccac tttcagtgaa taccgtaaat
     8521 atatcgaaaa agataaggct cttgaacgcc gatttcagcc ggttttaatt gatgaacctt
     8581 ccattgagga ttctatttcg attctgcgtg gtcttaagga aagatacgag aatcaccacg
     8641 gtattaggat taccgatgcg gcacttgtgg cggcggtaga attatcagcc cgttatatta
     8701 ccgatcgttt tctaccggat aaagccattg acttgattga tgaagccgct agccgtctgc
     8761 gtcttgaaat agattccctg ccggctgaac tggatgaagt cgaacgcaag attagccagt
     8821 tggaaatcga aagtcgggcg ctcgccaaag aaaaagatat tgaggaatcc cggcagaggt
     8881 acgaaaaaat tcagcaggaa ttggcgcagt tgaaagacga agccgccgtt ctacgcgctc
     8941 gctggcaaaa tgaaaaaaag catattactg ccatcagcga gttaaagaag caaattgaag
     9001 cgaaaaaact cgaagccgag aaagcggaac gggaaggcaa ctgggaaaga gccgcccagc
     9061 ttaaacatta cgagctagtc gagctaaaca aacaattaga agccgaaaaa gaagccttga
     9121 aaaatgtttc cggtaaacgg gcgcttttgc gagaagaggt taccgaagaa gatatcgccg
     9181 aaattgtctc caaatggact catatcccgg tctctcgttt agtcgagggt gaacgtgaaa
     9241 aacttttaaa aatggaagaa aagctgcacg aacgagtaat cggacaagat gaagcgatta
     9301 aagctgtcag taatgccgtc cgacgagctc gagctggttt gcaggatgaa aatcgtccgt
     9361 tggcttcttt catctttaca ggctcaaccg gcgttggcaa aaccgagctg gctaaagcgc
     9421 tagccgaatt tcttttcaat gatgaaaacg ccttgatccg cattgatatg tcagaatata
     9481 tggaaaaaca ctccgtctcg cgtctcatcg gcgctccgcc cggctatgtt ggttatgaag
     9541 aaggtggcca acttacggaa gccgtgcgcc gtaaacccta ttcagttatc ctcctcgatg
     9601 aaatcgaaaa ggcgcatagt gacgttttca atattttgct acaagttttg gaagatggtc
     9661 gtctaactga taataaaggg aacgtcgcca atttcaaaaa tagcataatc attatgacct
     9721 ccaaccttgg ctccgacctg attatgagac aggctggtga cgttgatgag aacaacattg
     9781 atgtcattta tgccaatatc caggaaactg ttatgtcaca actgcgacaa attcttaagc
     9841 cagaattttt aaaccggatt gatgaaatca tcgtctttaa accgctttta cctaaagaaa
     9901 ttcgcgaagt ggtaagattg cagttcaatc gcttgataga gcgtgtcaaa acgcgtggaa
     9961 ttcaggtcaa attatcagac gaggcttgtg atttaattgc caaagccggc tgggatccgg
    10021 ctttcggggc gcggcctatt aaacgaatta tccagaaaca gattcttgat ccgctcgccc
    10081 ttgaaattct ggaagaaaaa atccagcccg gcgatacagt caatatagaa attcgtgatg
    10141 gcaaaatcca tttcgtcaaa ggctaaacga tagaggaaca tttcgaattg ccattccgtt
    10201 aaagccgaat tatcgtgcga aaatgcttgt gcatcaatat tagatattaa ctgatttatt
    10261 taggaggctt tggattatgc gccgaatatt gttttcgtta ttgacaacag gaatggctgt
    10321 gcttcttatt acctgtagta aaccggacaa cactgctttc ggatattggg ataatgctgt
    10381 ggctctcttt aaccagtctg attatcaggg atgtatcaaa cagtaccaaa aaatcatcaa
    10441 atatttccct gatgacagtc tggcggtgcc ggctatgttc gcaatggctg atgtatataa
    10501 aaataatctg aatgatgccg accaagcgat taaaatttat aaagaaataa ttcgtaagta
    10561 tggtgattcc gaacgagctg ccaatgctca ttttatgatc ggatatgtct atgccaatga
    10621 gattagagat ttgaagaaag ccgaagaata ttataaatcc ttcctaaaaa agtatcccac
    10681 caatattctg gcgccctcag ctgaatggga actgaaaaac ctcggcaaaa ccctcgatga
    10741 aattcccgaa ttacagaaaa taacccaaga aaaacagact gcggagtaaa gctatgacct
    10801 tcaatttagc tgaaatgaat gaacgcattc agcgcgaaag tgcttttgtt tctaaaataa
    10861 agagtgaaat ggctaaggta atcgtcgggc aggaacaaat gatcgaacgt atgctgattg
    10921 gtttgctctg caatggccac attctattag aaggcgtgcc tggccttgcc aagtcattag
    10981 ctgttagcac attggctaaa attatccagg tcaattttcg ccgcatccag ttcacaccgg
    11041 accttttgcc ggctgatttg gtcggaacga tgatttataa tcaaaaaacc ggaacttttg
    11101 agactaaacg cggtccgatt tttagcaata ttatattggc tgatgaaatc aaccgctcgc
    11161 cagcgaaggt gcagtcggct ttgctggaag ctatgcagga acgtcaggta acgatcggcg
    11221 aaaccagtca tccgttggac gacccgttta tggttttggc gacgcaaaat ccgattgaac
    11281 aagaagggac ttatcctctg cccgaggctc aggttgaccg ttttatgctc aaggtgattg
    11341 ttgattatcc gcagaaagat gaagaactcg acattgtcaa acgtatggcg cgcaacgccg
    11401 ctattccagc ggtcaatccg gttataaccg cggaggatat tcgacgtgct cgccaaacag
    11461 ttgatgatat ttacattgat gaaaaaattc ataaatatat tgtcgatctg gtttttgcca
    11521 cccgcgaccc aaaggctgtt aacctggccg acatcgccga tttgatccag tatggtgcct
    11581 cgccacgtgc ttcaatattt ctggcgatgg ctggtaaagc gcaggctttt atcgcaggcc
    11641 gcggttatgt gacaccggaa gacattcgta cgattatctg ggatgttatg cgccaccgca
    11701 tcatcctgac tttcgaggcg gaagccgaag aagtgacagt cgaagaaatt atcaggcgca
    11761 tcatcgaaac ggtcgcggta ccgtaatttt ttccacaaaa tattatctcg ttatgctgtc
    11821 taaggaaatt ctcaaacagg ttcgtcagat tgaaatacgc acccggcatc tcgtcaatga
    11881 tgtctttagt ggtgagtatc attcggtttt taaaggacgc ggtatggaat tctcggaagt
    11941 acgtgaatat cagtatggcg atgattttcg ttccattgat tggaatgtga ctgcccgtta
    12001 tgctcatccc tatgtgaaaa ttcaagaaga ggagcgtgaa ctgaacgtta tgctggcggt
    12061 agatgcctcg cgctcaaatg ctttcggttc gcagggtaaa ttcaagaatg aaatagcggc
    12121 tgaaatttgt gcgctactgg ctttctccgc cataaaaaat aacgataaag tcggcctact
    12181 gatcttcacc gatcaggttg aaaaatttat tccacctcat aaaggccggt cacatacttt
    12241 acgcgtgatt cgggaaatac tctactttca gccacaatcg ccgcgtacta atattaaatc
    12301 gggactggag tttctactga aaggcttaaa acggcgcgcg atcgtctttc tggtttctga
    12361 tttcaatgac gaaaatttcg ccaaaccgtt acgggtattg aataaaaaac acgatgttat
    12421 cgcaattcat ctccaggatg ctcgtgataa aatattaccg ccggtaggct tattaaacct
    12481 gaaagacgct gaaaccggag atgaagtctg ggtggacacc tccgaccgcg cattcctcga
    12541 acagtatgcc gatttagtcg agcaacagcg tcaaaatttg cgtaatatgg cgcggggtat
    12601 gaagctggat ttaatcgaaa ttgacacagc cgtttcttac gttgaccctc tggttaagtt
    12661 ctttaagatg cgggagcaaa ggttcaggtg atttgaactt aaggttttca atgcagttac
    12721 gtcgctcaaa atttaacttt ctcgccctct ttagttttat ttttatttta tttcaattaa
    12781 cctcagctca gaactccgga attattatcc aaaccaccat tgacaccacc atcgcaacga
    12841 tcggcgatca cctccatttg cgaatcaacc tgcgctatcc gatcgggacg atttttgagt
    12901 tgccgttagt cgaggaaacg atcggccagt tagaagtaat tgataaccaa ctttcgccac
    12961 cacgcaagaa ggggcaaatt aacgagcagg agtggcagtt gactctggcc gtttttgata
    13021 ctggacaaat cacaattccg gcgctggaaa tcaaagctca cccagcgaac gacactgcca
    13081 gtgtgctgac ttttcaaacg gattcgaaaa ccgtgcaagt ctattcggtt ctaccgccag
    13141 ataagcgtga actgaaagac attaaaccgc ctttcccttt acgtcggagt atctcaggga
    13201 aagctatttt gttaatactt ctgcttctgc tgattgtcgg cggaatttgg tattatagac
    13261 gctgggagaa agcccatcca ccgctaatta ttgatgaaaa attcctggag ccaccgcaca
    13321 tagttacttt gaatcagctg aatgctttga aagagaaaat gcccaccacg cccgacgaaa
    13381 tgcgagccta ctactttcgc atttcggaga ttctgcggga atttttagaa cgacgctttt
    13441 ttatccgagc actggaaatg accacggcgg aaattgctga ggcttttaaa tatctggaca
    13501 tcgagcccgc gctgaccagg gagtggatag agcttttctc ggaactcgat attgtcaaat
    13561 atgccggtca aaccccggct gaaaaagata tagcggcagt attggaaaaa gcctgccatt
    13621 gtgttgaaag caccaaatat gaaacatttt tacggaggac gatgtgatag aagtacaaaa
    13681 tttggtaaaa cattacggtt cggtcaaagc cgtggataat atttctttca ccgttgaaaa
    13741 aggcgaaatc ctcggcttcc tcggaccaaa tgccgctggc aaaacgacca cgatgcgcat
    13801 tatcacgggt tatatgccag cttcagctgg gacggtcaaa gtggccgggt acgacgtctt
    13861 tgaacaaggt ctggaggtta aaaagcgaat tggctattta ccagaaaacc caccgcttta
    13921 ttccgatatg cgcgtgcgtg aatatttgac cttcgtcgct cgcatcaagg gtgtcaatcc
    13981 caaaactatc aacgccgaag ttgatcgcgt cgccgaacgt ctgtcggttt tagaagttat
    14041 ggataatctt attagcaatc tctccaaggg ttacaaacag cgagtcggtc ttgcgcaagc
    14101 tctgctgaat aatccgccgg ttctaatttt cgatgaacca accaatggtc tcgatccgcg
    14161 tcaaataatt gaggtacgtg aaataatcaa gagtctcggt agtgatcata ctgtcatcct
    14221 ttcgactcat attttgccag aagtcagtat gacctgtaat cgagtcgcca ttatcaatga
    14281 aggtaagttg gtcaagattg atacaccgga gaacttgacg aagcaattac gcggctcgga
    14341 atctattatt ctgcaggtcg agggaccaat gaacgaaatc gaaatcgccc ttagtcagca
    14401 tcaatcggta accgccgtgg cggttcaacc aacaggcaag gaaaatatcg ctacttatag
    14461 ggtggagacc tcaatcggtt ctgatattcg taaagagcta gcatctctag tggtcaatcg
    14521 tggttggggt ttgctcgaac tgaaagcgga aagcctgagc ctcgaggata tcttcctgaa
    14581 actcaccacc aaagaagagg aggtcaattg aaatgagtaa tttactggca atatataaac
    14641 gcgacctgaa aggatatttt ttctctccaa ttgcttatgt tgtaatcgga ttgttccttt
    14701 tagtcaatgg tatttttttc tacgtattgc tttctagttt cctggaatac agctatgcgg
    14761 taatgatcca gagacaaggt tatccgatta atgttaatct tcttatgatc cgaccgtttt
    14821 tccaaaatat gagcgtcatc gtgttgtttg tcattccgat gatcactatg cgcagttttt
    14881 cggaggagaa aaaaagcggg actatggaac tcttgttgac ttcgcctgtg accaattggc
    14941 agttgattct cggaaaattt ctggcatctt ttacccttta tttcgtaatg gtgatggtta
    15001 cttttctttt tatggttttt ctgttaatct ggggcaatcc cagcctcgta ccgattttga
    15061 ttagttacct cggaattctg ctgatgggct tagttcttgt tgctctcggc aattttattt
    15121 cctcgctgac cgaaaaccag atcgtcgccg cggtcggtac ttttggagct acaatgttct
    15181 tatgggtaat cggtttcgct ggagattttg ctggtaaagt gtttggtgaa atatttaaat
    15241 acttatcaat agtcaatcat tttgatgatt tctccaaagg agtctttgac actaatcatc
    15301 tggtttatta cctctcgttg acttttctaa tgttgttttt tacttatcag tcaattgaat
    15361 cttccaagtg gaggggttaa gtcatgaaaa tttataaatg ggttttttcg attggattaa
    15421 ttttaattgc ctgcgggtta atttactttt ctatcgtcaa tatctggtcc tggccggtga
    15481 ttgttttatt gattctcggc tttggtgcca gcgtgttcgc tttgattaaa ttggatatcc
    15541 gaactttgct gaaaaatcgg aggctgatct acggtggcaa tatggtgctg gtaattattc
    15601 tgattattgc tattgtagga ttagttgatt ttttctttac ccgtcattca tggcgggtag
    15661 ataccaccgc caccaagatc tttagtcttt ccgatcagac tcgtaagatt ctgaaaaacc
    15721 tggataaaga cattgagatt gtcgtttttg ccaagaaatc caataagtct tacatcaatg
    15781 atcgtctcaa tgaatatcgc cattattcca aacgcattag ttgggaagtc gttgatccgg
    15841 atgaaaaacc agcccttgct aagcaatata atgttaagaa ttacggtaat cttgtgatta
    15901 tttgtgaaga taaagcggaa ctggttccca atttttctga agaagcaatt attaatgcca
    15961 ttattaaggt aactcgcgaa ggtaagaaga aagttgcctt cttgaccggg cacggcgagg
    16021 gcactatcgc cagcaccgaa cgcgatggct ttgaacgagc caaacaggct atcgaacagc
    16081 aaaattatgc cgtcgaggag ctgatgctgg ttgataagga ttcgattccg gcggatatct
    16141 cagttttggt aattgccggt ccccaaaaac cgctgttcga aaaagaactt gatctgctga
    16201 caaaattcat cgaaaacggt ggtagtagtt tgataatgtt ggatccgcgt cccgcacccg
    16261 gtttgagcga ttatatgaaa gggtggaaga ttgaggttgg cgataattta attattgatg
    16321 ccagtggatt tggccgcctt ttcggtgccg ggccagatat tccgctcgtt actgactata
    16381 ccgatcatcc aattgtaaag gatatgaaaa atgtaatgac cttcttccca atggctagaa
    16441 cagttagacc gctggagtct gataacaaag agaatataat agtcgaggaa ttaatccgaa
    16501 ccggtcagca gagttttgcg gttgccaata tcgaggaagt ttaccgcact ggtcgcgttt
    16561 ccagaggtcc gaatgatctg cagggtccac tttcaatcgc ctgtgcggct acaattgaga
    16621 acccttccgg ccagcgtgaa ggtcggctgg tagttgtcgg cgattccgat tttgcttcga
    16681 atgcctattt cgataatcaa gctaatggcg atttatttat gaatatgatc agctggctta
    16741 ttgccgacga agacctgatc gcgattcgtc ctaaaaatcc ggaaattagc acggttaata
    16801 tgactcccgc ccagaccaaa acggttatgt ggctgaccgt tataattttg ccagcaattg
    16861 ctttcggggt ggcgattatg gtttatgtca ggaggaaata aaccatgaaa aaaggccgat
    16921 cgactctgat tttattcggg atttttgttg tcttgtttgc ggctgtttat ttttttgaaa
    16981 ttaaaggcgg cgagaagcgg gcaaagacaa gcaaattagc tagccaactc ttccagatcg
    17041 aaaaagacaa catcaaacaa ttgattctct ccggagatta tggagaaatt acttgcgaga
    17101 aagacggagc ggagtggatt atcaccgcac cggtgttagc ccagggcgac gcccagagta
    17161 tcgaatccaa cctcgattta atcctaggga ctagtatcga acgaaagatt gccgactcaa
    17221 ccgccgattt aagtttgttt ggtttattag aaccgcgcgg aaaggtgcag gtcgttactc
    17281 aaagtggtaa acataccata gtattgatcg gtgacgagaa tccaaccggc gacttgattt
    17341 ttgtcaaata tccggatgac aatgcgattt atactaccaa taaatcactc tggacttatg
    17401 ctaataaaaa actatacgac ttgcgcgata aaaagataat gcatttcaat ttagatgaag
    17461 tgcgcaaaat caatatcact tcccgggcaa aaggaaaagt cagtctggaa aaagtcgcgg
    17521 gaaaatggca aatcgttgac ccagtaaatc taccggcaaa tgatagcgaa gtcaaatcgt
    17581 tactcactcg cctatcaacc ggtagagcga aggagtttat tgacgaagaa ccggatgatc
    17641 ttaaaaaata tgggttgacc aatcctgctg ttatgatcac acttgaaatt ggcgaaagcc
    17701 tggcgcgtac cagtttttta gttggtgata ctgcaaccaa tgatggtggc ggtttttacg
    17761 ctaaagaaga aacccggcga ccagtcttta ctgttgaaaa atggactgtg gatggtataa
    17821 ctaagagtgc tttcgatttg caggataaaa agttacttgg attcgataac cagaaatgtg
    17881 atcggatcgt ctggcagatt gctaatcagg aatacattgc tacaaaaatt gattcagtca
    17941 attggatgat tgtatcaccg gagactctac aagttgatga aaacttgatg cgccgctggc
    18001 tggaggcttt gcgggatttt agcgttgacg agctggaatc ttatcaacct aaatcgctat
    18061 ctgtgtatgg tctggataag cccactttgc gactggcttt ttaccacaaa gattccaaat
    18121 cggatgaact tttagtcggc aaggaaattt ccgaaaagta ttatgtaaaa attggtaatt
    18181 atccacacgt ttatcgaatt aagaaaaaca cattcgagcg gatttataaa aagccagaag
    18241 atttgatgat taaacccgtt tctacggaaa gttaatatca atcaatttac gaattccctt
    18301 cctttgcggg actactttag gagaattcat ccgcattttt tagagtaacc atttaacttt
    18361 tacgtcggtc cttttcggcg aaatatttgc tgagcaggta taaagtcgag cgctcctgct
    18421 cggaaaggtg atcatagccg tcagtgctga ttttatctaa aatgtgatca agttcctcgc
    18481 gcatagtttc ttcggtttca taagagagag gcgcttgttc tctgtccctg gacttttttg
    18541 attttttcga ctgcaaatta aggtgcagat tgcggaaggg atttttgatt ttggggcgtg
    18601 gccaatgcca gtttgccgaa cccgtccgac gatgccaacc ggagagtaat ttgtaacgag
    18661 ctaagtaaag ataaccaatt accaatccgc cgagatgggt tagatgactg atattgcctg
    18721 tccccgggga caaggtcgaa aagaaagtaa ttacgaccat aatgatagca aaatatttgg
    18781 ctttaatcgg aatcaggaag taaaagtaaa tatatcgttc cggaaagagc atcgcgaaag
    18841 ccacgagcac accataaatt gccccgctgg caccgaccac cggaatgtca gaatgagcgc
    18901 tgaatagcat cgttatcaag cccgaaccaa ctcccgttat aaagtaaaat ttataaaatt
    18961 cgcgtttgcc ccaataattc tcgatttcca tgccgaacat ccacaacaca aacatattcc
    19021 agaaaatgtg gccgaatcca ccatgaataa acatgtaagt aaataattgc caaacatagc
    19081 cctttttcag gacggcgtag ggtaccaatc caaaattaaa ggcaaaaaaa ttctgagcct
    19141 gaaaaatcac cataatcaaa taaatcacaa cattagcgat aatgatattg cccacggcgc
    19201 ccctcgaaaa acgcgggcga atataaccgg atttataacg cgattggttg taattgtaat
    19261 aattcattgg ctaatccttt ctatcggctg actggataat ttgcgagaga agttcgaagc
    19321 ttttcaatct gacgggtaaa tcggtttgca ttgctaaata attcaccaaa gttttggcaa
    19381 tcgccataat tggcaaattt aggttgcaga gcggcaaacc ggtgcggata tcagcttttt
    19441 caattttttt cagaatctca agttcctcca tagaaaaagg taaagttgac cagtccgaat
    19501 ttctactgga attgagaccg atctcgtatc ccatcagcgt cgtcaaattc aatataaaat
    19561 aaacaaaagc aagttggcta ttatcgttct gcgcctcgat ataattcaaa gtgtccaaaa
    19621 ccaatttaaa aatcggtaca tcctcatggt gctcgcgaat gaacttttca atacattcca
    19681 aaatggcggt tgccataatg gtcggctcaa tttcagtact gttggacaaa aaactccgga
    19741 tagtttcggc ttggctgagc gtctgaatat cgcgagtcgg cttgtaataa aattggactt
    19801 ccagtaaact tagtggctcc aagtacccgc ggaaagggct tttcaggttg cgagcgccct
    19861 tggcaagaac cgcaacttta ccgtttgatt cggtaaacag acgtgcaatc aagctagtat
    19921 ctttaaacgg aatagttttg attacgaagg cgcgcgttct aattattgcc attattttcc
    19981 tctggattaa tcctaaatca agccatctcg atcacatttt cttattgaaa ataaaagtat
    20041 tcaattttgg actttctgta ttagtcacaa taaatcataa taaatcacaa tgaatgacaa
    20101 cgaaacacga ttaatgacaa tttcctgact cccgaaatac taccaatgac gactgagctg
    20161 ctaattgatt gccgtcgtta aattttaata agctcgagca aagatgacga tccggtcagt
    20221 ggttttgccg gtataaaaac aagtgccttt ttctttttgc tcaaatggaa tgacccgaat
    20281 agtggctttg gtctcttctt gaatgcgagc ttcatcagcg ctattgcccg cccaaaaaac
    20341 ccggatgaat ccacccgcct cgattaactc tttgaattgg tcataattct cagcctgaaa
    20401 ggtgttattt tcgcggaagg cttgggcgcg tttgaagagg ttagtttgaa tttcttccaa
    20461 taagttttta ataaccgtcg gtgcattatc aaccggaata ggttgtttac gtccgatggg
    20521 ttccatctcg gtcggatcac gacgaacaag aacgacctgg tttttagcga cgtctttggg
    20581 accgacttcc agacgaatcg ggaccccttt catttcataa taattgaatt tccagccggg
    20641 agtttcctgt tcggagttat cataacgaaa ccgaatttgt tcggctttca atctggtggt
    20701 gagattagcg acaaattcat tgattgcgca tttttcttct tcgcttttcc agattggcac
    20761 aattacgact tgatacggcg caacgcgcgg cggcaaaatt aatcccttgt catcgccatg
    20821 cgtcataatc aatccgccga tcaagcgtgt gctgatgccc cagctggtag catagacata
    20881 ttcgagctgg ttggatttat ttaaaaaagt gacgtcgaaa gctttggcaa aattctggcc
    20941 gagattatgc gacgtcccag cttgtaaggc ttttttatcg cccatcattg cttcaatagc
    21001 gtaagtatga accgctccgg caaatttctg cgcttcggtt ttacgaccga ccagaaccgg
    21061 catagcgaga taatcctcgg caatctggcg gtaaatctcg agaatgcggc gagcttcctc
    21121 ttcagcttct tccggtgtag catgggcggt gtgtccttcc tgccagagaa attcggtcgt
    21181 tcgcaaaaaa aggcgggtgc gcatttccca gcggacaata tttgcccatt gattgatcaa
    21241 gattggtaag tcacgataac tgcgtatcca ttttttataa cttgcccaga tgatcgtttc
    21301 ggaagttggg cgcacataca gcggttcttc aagaggtttg ccgccaccga tcgttacaat
    21361 ggcgcattcc ggcgcaaagc cttcgacatg ttcggcttcg cgcttcatat aactttccgg
    21421 aatgaacatt ggaaaatagg cgttgacgtg accagtttct ttaaacatcc gatcagcgat
    21481 gctctgaata ttttcccaga tcgcataacc gtaggggcga ataaccatcg tgcctttgac
    21541 cggaccatag tccgccattt cggctttcaa aacgacatca gtataccatt tggcgtaatc
    21601 ttcggaacgc tttgtaacac taattgacac gttaatttcc ttgatttatt tcgttgtttt
    21661 tttctttgaa accgatccga tggaagggat tgacaatact ggtgcaaata ttatagaggt
    21721 gcgccccgac gcgtttcaaa aaacgaatgt atagcgccag agtcaccgca tcggaaatat
    21781 tgaattcgca ttcgcctttg ataagggtat tgattaaaat agtcgtatcg cgggaaacat
    21841 ctttatactg atggacgacc tggcgcgcca actcaacatc cgattccgga aaggctttga
    21901 ttgtccagtc aaaaaagtcc tttacccgtt tttcgatact gactacagtc tcctcaaact
    21961 cggcggcatg gagagtcttt ttatgcagaa ttgccaaatc agcgatgttt ttggtataat
    22021 caccaatacg ctcgatatta ttgacgacaa tggcaagcgt taatccggaa atgacctcgc
    22081 ttttatcgga aatcatcaga tgggtcatca attttttacg aatatcccgc accaatttat
    22141 taatagtttt atcatgttga taaatgtcga ggcgcaattc cccagtgtca ctttcgcgta
    22201 gagaccacat tgattctaca aacatctcgc gggtcttagt gagcatatca agggcttgat
    22261 ccacggtttc cgcagtcaaa ctcttttttc gccaaatttt taaaaattca tcaaacatat
    22321 cttacctcgt tataaatata ataattaatg gtatgataaa gaaaagcaag ataacatagg
    22381 caatggcata taaccggttt ttacgggtta aactggctaa taaccgggct agctcaatgg
    22441 ggattttttg gaggggtagg aaaatacatg cgccgattac attgaataaa gtatgagcaa
    22501 aggcaatcgt aatagcatca accgaattag tggctatagc ggcaagaaaa gccgtgattg
    22561 tcgtaccgat atttgcccca accgcatagg gaaaaacctt cttttgcgac aatactccgg
    22621 cggccgccaa cgggaccgtt aaactggtgg taatggaact actttgaact atcgcggtca
    22681 gcaacaagcc cagtagaaag ctgcgattag cgcttttaaa aatatactga tcaaaaaaca
    22741 gctcaacttt agcgagaacg agcgatttaa tcactttgac taaaaaggtc agagcaaaga
    22801 acagaataac taaagccaaa actatattta tccagggata attgggaaat aaatcaataa
    22861 taaattgcga taccggttcc aaaattttac taattggatt gataaacttc aaagcgccga
    22921 tattttcaaa agcagttgcc acagcaccgg cggcaattcc gagaatattg aagaaatatt
    22981 gaaatgggaa tatgatgatg acgcttaaga tattgaaaaa atcgtgcatc gtagcgaccg
    23041 caaatgctcg ctcgaactca tcctttttcc caatatgtcc cagtgccact aaaacattgg
    23101 tgatggttgt gccgatattc gcgcccataa tgattggaat tgccaaattg attgtcagaa
    23161 cattaccggc taccatactc actactaaag aagtcgtcaa tgaggaactt tgcacaatag
    23221 ccgtgcaaag catcccagca aaaagaccca cgaacggatt agatacgccg ttcataattt
    23281 tttgggcaaa gccctgaccc atcattttaa aggtatcgcc aatcagggtg acacttaaga
    23341 gaaaaagata aatcagcatt atgattgcca gcgccttggc aaatccaacc caatatttcc
    23401 tatccctaaa attcattcca gtccttttat atgattaaat aatttcaaaa atcgggcgga
    23461 atttagaaaa aaatcggcta ataaccgcat tttttggtta gtctcttctt aattattttt
    23521 acttgttttt aagttatatg ttaaatttta ataatgagtt gaagtaatac tcctaatcca
    23581 cccatccgtt aatctttctc aaatgaactt aaaatcactt aatcgcactt taacgtgcat
    23641 tattagtgcg tgttttgtac aaaaatcttt cctaactttg gatgtttttt ggatattttt
    23701 gtaaaaataa ccactttatt tataaaggga tcggtatgaa atcgtccaac atatttgatg
    23761 tcatattaat cggtgctgga ccggcaggtt tatttgcggc tgaaaccctg gctggcaaaa
    23821 gtttatcagt gctggtttta gatcgcggtg atgaaccgtt gaaacgtcat gatgtgaatt
    23881 tcggaattgg tggcgccggt gctttttcgg acggcaaatt gaatttgact tctcggattg
    23941 gtggcgaacc ggaaagtttt ggtcgcaaag ctgatgaagt ggaggaattg attcggcaga
    24001 tagatgatat ttttacttca cttggagtaa tcggtggtta ttcaggagaa gatggcgagg
    24061 cttatcaggc tttattgcgg tctgcccacg ccgccggggt agagtttata tttgccaaac
    24121 agcgtcacat tggaaccgac cgcctgcagg atattatcca acgcttttat gaacgattaa
    24181 ttgaaaaagg aattaatttt cgtcctaata cctatgttga taaaatttct aaagagggtg
    24241 attattttaa attaatctgc aaggatgagg ttctttttgc cagagcagtg attgcggctc
    24301 ccggcagagc gggcgcttat tggttgcgcg aacaagctca taatctcggg attcacacga
    24361 tgtatggacc gattgatgtt ggagtgcggg ttgaatttcc agatgatatt tatcgcccaa
    24421 ttgccgccgt tatgtatgac gctaagtttc gactttatag taaatcttat gatgatctgg
    24481 tacgcacctt ctgcacgaat ccgagtggct atatcgttac ggaaaaatat gacgattttg
    24541 tgttggttaa cggtcatgcc aaatggaatt ccaaaaccga taacaccaat tttgcattac
    24601 tttcgcgagt cgtcttgacc gacccggttg aggatacgac caagtacgga cgcgatattg
    24661 ctcgtctggc taccacaatc ggcggcggta agcccattat ccagcggctg accgactttg
    24721 tttcgggcag acgctcaaat tgggaacgca ttagccgctc cgctacggta ccgactttga
    24781 aggatgtcac gcccggtgat attgcgatgg cttttcctca ccgcattgtg accaatttga
    24841 tcgaagggct taccgtatta aacaaaatta tttccgggct aatgtccaat aacactttgc
    24901 tatatgcccc ggaaataaaa ttttacgata ccaaatataa agtaacgccc tatttagaaa
    24961 ctacacttga aaattttttc gtggctggtg atgccagcgg acatagtcgg ggcattgtct
    25021 attcggcggt gaccggtatt tttgcggcgc aaggagtcct aaaaaacctg ggcaaagatt
    25081 aagttcagcc tgtcattttt taattcgatc tagtgcgagc tgtataagga gagaaaaatc
    25141 ttgaaatcgc cggagaataa agataagttc atgcgagagg gactcgagta catcaagcaa
    25201 atagttaacc gtcgtggtag tcgcgacact aaactactgg aaatttgccg ctttctcaat
    25261 gataaattta tcggctttga ttgggttgga ttttacttca gtgataatct caaaaaggag
    25321 ctaattttgg gaccgtttat cggagatccc accgaacaca ggaatatccc atttggtcgc
    25381 ggagtttgcg ggcgtgcggc ggtttcgggc acaacggtca atgtggctga tgttacggct
    25441 gaatcgaatt atctggcttg tagtcttgag gttcggtcag aaatcgttat ccctattttt
    25501 aaatccggtc agttcgtagc tgaattagac attgattctc atcaatcgga tcggttcggt
    25561 aattccgaaa gacaatttct ggaacaagtg gggcaaatta tatcagttat tttttagata
    25621 taggcaacaa ttatgctaat aaaaggttaa attaaggttt gatttttaat ttgctattta
    25681 aatgatttat aaaagaatta actatgttag attgagacaa attatgcgca aggcaaccat
    25741 tccggttata ataattatcg ttgtgttagg atttttattt atttccccgt ttggcaagtc
    25801 aatcattgcc tcggggagtg acttatacca aaaattaaac atttttcagg atattattcg
    25861 aattataaat gacagctacg tggaagagcc ggattggaat aaagtaatgg aaggcgctta
    25921 ccggggaatg ctggaagaat tagatcccca ttcggtttac atcgagccca aacaattgga
    25981 tgatctaaat gagcaatttt ccggtaaatt tgagggaatc ggtatcgaat tcgatatttt
    26041 ggacggttat ataacggtca tatcaccggt tgccggagct ccggctgaaa gagccggact
    26101 ggaagtcggc gataaaatcg tcaaaattga tggtgaatcc gcctatcgta ttagtcgcga
    26161 tgatgtccag aaaaaactgc gtggtcccaa aggctcggaa gtaaccgtta cggttcgtcg
    26221 acaggggcaa accgattttg atgtcaagat tatccgggat gaaattccgc tttatagcgt
    26281 ttcggcggct tttatgatag atgatagtac gggatacatt ttcttaaatc ggttcagcgg
    26341 aacgaccagt gacgaagtcg aaaaggcttt aaaccgattg gctgcggaag gcatgcaacg
    26401 tctggttttt gatttacgta ataatagcgg cggttatctg gaacaggcgg tggaaatcgc
    26461 tgataaattt atcgccggtc gccaaaaaat cgtttatacc aggggacgaa tttcgagtgc
    26521 taatgaagaa ttttattcga gtaaaaccgg accctatgaa aagatacccc tggtaattct
    26581 gattaaccgg ggttcagcca gcgccagtga aattgttgcc ggtgcggtgc aggatctgga
    26641 tcgcggacta attgtaggcg aaaccagttt cggcaaaggc ctggtccaac gtcaatatcc
    26701 attgagagac ggctcggcaa ttagagtaac tgtcgccaga tactatactc ccagtgggcg
    26761 gctgatccag cggccttacg atggggcaat tgacgactat tatagcactt ttaatgagga
    26821 aaatcgtgat tccttgttag ctatcgagga ttcggttgag aaacggccgg aatttagtac
    26881 cattggtggc cggacggttt atggtggcgg cgggattacc cctgattatt cggtgaaata
    26941 tgatcaagcg ttgaataatg agacctacaa attactgaga catccctcgc gagttatttt
    27001 tgaatatgcc tccgattttg ttaataagaa tcgggattta gccgccgatc gtaaaaattt
    27061 ttataaaaat tttgaggtta gcaataaaga tttcgaggca tttaagaaaa tggcggtcga
    27121 aaaaattggc gatttgaata ttaagcaagt tgagaaagat gctgaatatt tgaaaatttt
    27181 aattaaagcc gaaatcgctc atggttattg gggctacgat gaatactata agattgtgcg
    27241 gctttcggat aatcaagtca cgtctgccgt tgattttttg aatgaagccc gaaaaatgct
    27301 tcagattcaa taattattaa ttgagaatat cattgacttt tttaaattat ggagttaaaa
    27361 ttgtcagcat taaaaattga aattagaatt gtaattatgg aggtaaataa ataatggttg
    27421 aactgtttct tagaggcgga attttcatgt ggcctattct gggattagca atatttggta
    27481 taatggtcgt gattgaaagg gcatatacac tgtctaaggc ttcgcggata accaaacgct
    27541 ttcttccaaa agttcttaat actttgaaag aagaagatgg agtcaagaaa gcgattgagt
    27601 tatgtgaaaa tactcctggt ccgatagctg agatcttcca tgctggttta tcggcagtag
    27661 gaaatgggat ggaagccgtt gaaaaggcga tccagagtgc tggaaccgtc gaaatggcat
    27721 ttcttgataa aggtatgatt tggctggcga ccgtcattac cctggctccg ttactgggct
    27781 ttatgggtac ggtttctggt atgatcaatg ctttcgacgc catcgcaaaa gtcaatgata
    27841 tgtcccctgc tattgtagct ggtggtattt ctgaagcttt attaacaacc ttatttggtt
    27901 taattgtagc agctccgcta cagttatgtt ataatctatt cgtagcacgc gtggacagta
    27961 ttgtagtcga gatggaagaa agtactatga atttattgca aactcttgat gttattaata
    28021 aggaaaattg caagtaaata tggctctgct gaaaaaaaag aaaactcgca aagttgatat
    28081 tccatctgcc tcgcaggcag acatagcctt tcttttactc cttttcttta tttgcacgac
    28141 cacggttgat gtagataaag gcattggact gatactccca cccgaaggtg acagcccggt
    28201 agagatttct aaaaagaaca tcactacaat cgtaattgat ccgcagggta acgtgttaat
    28261 tgataataaa gaagttgaaa ttaatcagat tcgagatatt gccgaaagat tacagatgga
    28321 aaatcccaaa atgatttttt cggttaaagc tcacccgcgg tgccgatacg ataaatacgt
    28381 ggctgttttg gatcaattaa aaatggctaa agctaccaga atttctattg ctgaataagg
    28441 agtactgaaa tggcttttca acacaaaaaa aatatcagta cggaggttcc aacagcctca
    28501 atgccagata ttattttcac gttaacctta ttcttcatgg ctaccacagt gatgcgtcaa
    28561 tattctggtt taccggtagt tttacccaaa gccaaacaaa ttgagaaact tcagtccaag
    28621 atccatgtaa ccgagttgtg ggtttctaaa gaggaattga tttcaataga tggtaagctg
    28681 gtaaatctcc aggacattag aaatattatg tataacaaac gtgttagaga tccgcagtta
    28741 attgtttcga ttcgggctga tgaaagggtt acgatggact taatgagcaa gattcataaa
    28801 gaactgcggt tagcggatgc gcttaaagtc aattattcca ctaaaacagc tagttaaggg
    28861 ttagactatg attttaagaa aaaaacccga agcaagtttg aaattacgcc aaccgctgat
    28921 gtatcgagtt ggactggttg tcgctctggt attaattaca gggattatga ttgctttccc
    28981 caaatttgaa gaacgcgaag tagtccagag aaaagttgat gttattttgg aagtaactga
    29041 tattccgcaa attgaacaaa aaatcgaagc cgccaagcca ccgtctcgtc catccatccc
    29101 gatcgagtcg gaaaacgaag acctttccga agacattaca attgatcaga cgaccgattt
    29161 tgattcttat acagcctggg aggcaccacc accgcctccg gaacaacata gcgacgaggg
    29221 tgaaggcccg cgcgtccgat ttattcccta tgatgaacct cccgagccga ttggcggatt
    29281 tgccgagatt cagaaaaaca tagtttatcc agaaattgcg caggaagccg gtatcgaagg
    29341 gacggttgta gtccaggctt atgtgaatga atacggagtc gttaccgaat gcaccattct
    29401 aaaaggaatg cccaatacgg gactcgaaga agccgctatc gccgcgatta aaaaaacccg
    29461 cttcaaaccg gctaaacaaa gagaccgaaa cgttggtgtc tatatttcga tcccggttat
    29521 tttcaaacta aaaagctaat caattagtcc taaatattta cctgaccggc ataatctcaa
    29581 ttatgccggt tttggttttt aattattagt cgaaattctg actggttaat ttgcttcatt
    29641 ggtgaagaca atctccttgg tttttatcta tttattaacg ataccaagag tctattgaat
    29701 gtttttcttc attactcgtt tttttcgttt aaatcatttg ccaaatttgg actttctgtg
    29761 taaaatgaat tgaagattta gatcaaagtc aacgggacta agtgaattta agtaacccta
    29821 cagagaaccg tcctatttat gccaagactt ggcagaatgt agccgctgtt gaacgctatg
    29881 ctcttagccg ctatcgtcat tatgatcagc gcttaatttc ctggcgggaa aaacggatcg
    29941 ttaatcatct tctaaaacaa ataaaaaatg gaaatcacat attgttagat attccttcgg
    30001 gttacggacg ctttacccct ctctttcgcc aacacggttt caaggttatc aatggcgacc
    30061 ttaatttgta tgctctactt tatcaacggc aacgttttcc cgatagtaac ctgatgttcg
    30121 taactgatgg caattgttta ccgttgcctg ataactcagc cggtgtcgtt tttaatttca
    30181 gacttcttca acatttccag accagtgacc agagaattgc actcttacga gagcttgcgc
    30241 gggtcacaaa ttgttacctg atcgtatcgg tttatcttga atcgaacttt catcgtctag
    30301 ctcaattagt ttcacggcgt aaacgtcgga tgactatgat ttctcgaact cagtgggata
    30361 ccgaattgcg ttcgtgcgga ttaaaaatac tccggattag tcgtccgctt cgttttctcc
    30421 acgctcaaaa cattttccta ttggagaaat cgccaataat tgaaaaataa ttaactgctt
    30481 ataaattgag gtgaaggatg aaaaagttaa taacgattat attcaccgtc atagtattag
    30541 caacctcagc caagccgcaa gatttgttga ctgtatttac tgagccttat ctttccggct
    30601 cggtagcgga taacgtaacg tggtcgccga atgattccct gattgctttt ctatggaatg
    30661 atcaaggcgg agaatttcgc aatctctgga ttgctaatcc gcaaacaggt gcgaaagaac
    30721 agctgaccga ttttaatccg actacaaatg taaatctgcc gactatagga acgctcggtt
    30781 ggctaaataa tcgttgtctt ttctttgagt atcaaggcga catttgggag gcaagtctgt
    30841 tgccaggtta tccattaaaa cggatcgaag gcttaacgaa ttgcaaacca ccaattgctc
    30901 ttgcgccgaa tagaagatat ttaacctaca acagaggtgg ctatcttaga ttgtttgatt
    30961 ttataactaa agaagaacag acgattattc cggataaagc ttatcagcgt ccacccgcca
    31021 gacaggtgtg cgcttaccag actacttatt gctggtcgaa tgatagtaaa aaggttgcgg
    31081 tctatttaga atcggacgcc gatgaaaata ctgttcattt taaagttttc gacgtactga
    31141 aaggttcaac cgatgatatt gtgattaagg agcgtccgaa tgacaattcc attgtgcgca
    31201 actttatctg gtcgccagat aatcagtcaa tagttattga atcggttact tctaatttac
    31261 gtgaaagata tatcctttta gttgatctga cgaaagaccg attggatact ttatatcgtg
    31321 aaattagcaa taattggatt gcggatttcg gataccaatt atattggata gagtcggaag
    31381 cgaagatttt gtttgggatg gaatataact cttataatca tttatttctg cttgatccga
    31441 ctaatgggca ctattccgcc ttaactcgtg gcaaatggga cgttaaggat tatgctgtgg
    31501 atgctactgg gcagttggta tattttacag cgacaaaaga caatcctgaa caattgcaat
    31561 tatattccgc ccaaatcaaa acgggtgaga ttacagatat ttcttatcga ggtggtgatt
    31621 actcattcca attatccaat aatagtcgtt ggatagcgga ggttttttcc aattactcta
    31681 ccccgcccga tttatattgg atcgaagcgt ctccccaaag taaaatgaac caaataactg
    31741 atcgtccaga ttcaccactc gaaaaaccgc tgaggaaaat tccgaagagt ggaacaattc
    31801 agaatgcaat aaccggtaaa ccagttaatt accgcgtatg gttacccgac gattttagag
    31861 taaatcataa gtacccgatt atcgttgtat taaatggtgc tgattgccta gcgccgctga
    31921 ccgaagcctg gcgaccggaa tgttatttta agcaatggct gagtgataaa ggttatattg
    31981 ttgttgctgt gcaatatacg gatttaagga cagctattga accagcggac tcggaatctt
    32041 tcagcgatcc tttgaaaatt caattagcag atattcaggc tgttgtcaaa gaaatcagta
    32101 aacttcaata tgttgatttg tcaagaagcg ggatttgcgg ttggggctat ggtggctact
    32161 tagccacgat ggctatgttc gaaactgata atatctttag agccggggtt gcgattttaa
    32221 atgacttttc ctggtcacaa tcatcgagta tctatttgaa ttatatggct aatctactag
    32281 ctaatgacca ggtttttaga aaaattgatc cacataattt ttgctataat ctaaggggac
    32341 gtttgcttgt tattcagagc gcatccagca ctttacctca attagtagat gcccagcggt
    32401 taattcctga attgttatct atccgtagac cggtagattt ttatctttat ccttgggaaa
    32461 ataatccgat tatgaggagc gccaatcaat atgatatgtt tattaaaacg gcgcgctttt
    32521 tccagttgaa tttatgactc tggctcagaa atgaggttgt tcaattaggc aatttgcttt
    32581 cttccgaatt gggaaatcca aaaggttcaa aagattcttt gtccgggagt ttgatctcac
    32641 caaatttggc ttcgaacttg gcgaggttat cctgcaaagt gaatagtaac gtcttggcgt
    32701 gaattggagt caggattatt cggctttgca caacggcttt gggtgagccg ggcatgatgc
    32761 gtgcgaaatc aagcacaaat tcggcgttcg agtgcgaaat gagtataaaa ttactgtagt
    32821 taccttctgc cttggattct ggcaattcaa ttttaatttg cgcgacaggt ttagttgagt
    32881 tcattaatct ttccttaaaa tgatacgagg gagttaatat tcatcttcct cgcgatcttc
    32941 attgtcttcg tcccattcgt cttcgaaggg aatgtcagta tctgagagat cccaggcgtc
    33001 atcactgctt tcaatttctt cagtgagatt ttcatttaac tcaaggtcgt cctcgaattc
    33061 atcccagact tctttctttt tctcaaattt ggatggatca atttcttctt cgtcgaaatc
    33121 ctcgcgttcg tgttcgagca tttctttgcc atacaggctc atatcgaaga aatcaatatc
    33181 atcaataatg tcataataga ccaacagttc cagaaaatcc agatatttgg aatcccgcag
    33241 gttagtcttg caattcgggc aaaccagaga ttgttttaaa tcttcctctt tgagaggcgc
    33301 attacattcg gggcaaacta aatcttccat ataatttcac cttatattac ggcgctaaat
    33361 atacacgccg aaggtatatg cgtcaagtta aatttttatt tactttttgg atttgcggtt
    33421 gtaataaatc ccaaataaga ttgtaggatt tttcaactaa tctatccgtt ttataattaa
    33481 tgattaggtt gaataatttt tccgctaatt cgctttgaaa accgaccttg atcggcgcgc
    33541 ggcagcggga aacgataaaa gacgtaggaa ccgtcgggac cacatttaaa tcaattgcga
    33601 actcgaggtt gattcgagct aatgccagta cttctggatc ggtgggaaag ccctctttgt
    33661 tgatccgact ggaaatcaag ggtatcaata aatcgggatt aatttcctga attaaggtaa
    33721 aaaatgcttg cggtacgagc aatctgacca actccggttt ttgtcgtatg aaaggtaaaa
    33781 aaactttcag tgcgctatag aaatgagcgt gctcaaaggg taagcaaatt agaactcgag
    33841 cgaatttagt tgattccgat tcctgtaaag acgtatccag aggccagcta aatattttat
    33901 gaatagcctt atgattcaag acattaaatt gttgatagtt agtacaattc atcaaattca
    33961 accttaaaaa ctgagccgaa aataaccttt aatttacaat tttccaataa tgggaaattg
    34021 ttaccgagtc atttaacatt caaatatatc aattatgcag taacacaata agtttcttat
    34081 aaatagtcga gataattagc ccgagtccct aatcaatctt cgggatttca cagttaatct
    34141 ctttctttta agcgcacaat cgtcgcaccc cagccgccac cggattcatc ggctaactg
//
LOCUS       DAOOMU010000010        34041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1045_length_34041_cov_20.107986, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000010 DAOOMU010000000
VERSION     DAOOMU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 34041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34041
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1045_length_34041_cov_20.107986"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1869
                     /gene="pgi"
                     /locus_tag="PLI55_01780"
     CDS             196..1869
                     /gene="pgi"
                     /locus_tag="PLI55_01780"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020530594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPA99599.1"
                     /translation="MLPSVNPTTTSAWQKLSAHYQEIKKISLRNLFNEDPERFQRFSI
                     NLVLDETSRDEILLDYSKNLITPETFRLLLKLARECGVAEAIELMFTGAAINVTENRA
                     VLHTALRNLDDNPIYVDGQNVMPPIKAVQEQMREFSAKVINGEWRGYDGQPIKDVVNI
                     GIGGSDLGPAMVTEALKPYHIPHLKLHFVSNVDGTDICETLKCLNPATTLFIIASKTF
                     TTQETMTNAQTARNWFLTTVGEPDFIKSHFVAVSTNRAKVIEFGIDPENMFVFWDWVG
                     GRYSLWSAIGLSITLAVGFDNFRELLAGAYAMDNHFRRTPFEKNIPVILALLGIWYNN
                     FFGAETLAILPYDQYLRRLPAYLQQADMESNGKRVDRNGKVVNYQTGPIVWGEPGTNG
                     QHAFYQLIHQGTKLIPCDFLAPAISHNPVGEHHNILLSNYFAQTEALMNGKSEEEVAR
                     ELKSKGLTESEVQRQLPYRVFPGNKPSNSILFKKLTPRTLGALIAMYEHKIFTQGIIW
                     NIYSFDQWGVELGKQLAGRILPELANDQPITSHDSSTNGLINAWKRLRK"
     gene            1890..2699
                     /locus_tag="PLI55_01785"
     CDS             1890..2699
                     /locus_tag="PLI55_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPA99600.1"
                     /translation="MQWFGELSPVFQAFLATCFTWLVTAGGAGLVIFFKEVSQRLLDA
                     ALGFAAGVMIAASFWSLLAPAIEMAGASGSPAWIPAAVGFLLGGAFLKSVDRLLPHLH
                     LGLPIDQAEGIKTNWQRTVLLVLAVTIHNVPEGLAVGVAFGAAGAGLPSASLAGAVAL
                     ALGIGIQNFPEGVAVTVPLRREGLSIGKSFFYGQLSGFVEVISGVIGAYLVLTIQALL
                     PYALSFAAGAMIYVVIEELIPESQKDVSTDVATLGAMLGFTIMMILDVALG"
     gene            complement(2902..3753)
                     /locus_tag="PLI55_01790"
     CDS             complement(2902..3753)
                     /locus_tag="PLI55_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPA99601.1"
                     /translation="MNGQIPVLMVNGESLAAAWEKSLVELYQKGCDIKTEYDKPSDPP
                     SKDCTMMIVVQNPLAEPMIHCDFPGGLTDLQEYVLEVLEGIKDHCIRSAENPADARWE
                     YTYHQRLFNYQVPGFAKVYDQIAIMVAKLIKVPHTRRAQAITWKVWEDNDCYDPPCLQ
                     SIWCRLLKDDNSIWTLNTNVRFRSNDAYKAAFMNMFALTQLQVEIANRIEQSIGEKIN
                     IGRYVHQADSYHLYGSYFDEFQNRFLKALETRSFEERTFRYADVKSIMTEAIPQIRAK
                     AARMGRE"
     gene            complement(3942..5075)
                     /locus_tag="PLI55_01795"
     CDS             complement(3942..5075)
                     /locus_tag="PLI55_01795"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99602.1"
                     /translation="MKIIKTLLITLLLGGFANLKSDTFEQQEERVQIALKAMYNREYK
                     RAASLFHQVLNSTPFHPMGPLGALAVEYYQNEGLIGYHQRNQQFLVDIEQALQIYSEQ
                     IKQNPSHSEYFFFYGTVMGLRARIQLAEKNYFGVLTSGYNAIRFIKKTEKLYPDQPDF
                     QLPVGLFNYYVSISAPYMKIASRLMRESGSRSEGLQAIKAAADYGNYGRYEARSLLAY
                     LYFYFESNYDGAYEYARKMAEDFPKNPYYAAQTADAAIQIGQLDQANYYLKRIEALLP
                     TQPPRTQKDYQGILRLLYGSLALAQGRLDEAESNLIEFIKSYDYELDYDLGNALVRLG
                     NVYDLQKRRSEAIACYRKAAQLNNRTAAVSLAEQYLKRPYIRS"
     gene            complement(5167..6099)
                     /locus_tag="PLI55_01800"
     CDS             complement(5167..6099)
                     /locus_tag="PLI55_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HPA99603.1"
                     /translation="MIDAKNLSGLDKATILFKALGPDLSVQLFKGLSRSQMQKIRENM
                     AKIANIPFDVKKAVLEEFYFSFVSEKFIPAEEETKKPFEFMLKLTDEQIAYLIASEPP
                     IIMALAIAQLPVERQVNVLQKLDPTVQPKVLTEMGHLTEIPLEGVVSIANNLKDKSAF
                     IPRASEFKRGGGQDVAGILSKIPPRDEKRFLEFLSKEAPELVDQIKRYYFSFEDLPKL
                     PPNILAEVLKSVEISEIAYALKGQPDEIRDLFKSLLPQRSQIILEDEMKLLEGPQPRR
                     KVEAAQKKIVEKLRELEKEGRFNLEDFMDTDFIE"
     gene            complement(6112..>7221)
                     /locus_tag="PLI55_01805"
     CDS             complement(6112..>7221)
                     /locus_tag="PLI55_01805"
                     /inference="COORDINATES: protein motif:HMM:NF019944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HPA99604.1"
                     /translation="FDIPTSVIQSQERIQSQTQLDALPQPATGTAKSTSTTEKKVATS
                     VPVIKRQEINVILEDGISPEIIENVRQVVAVAAHYDRARGDVISIMTANFKKSPDKDA
                     TETIILKNIAEKIDDIERRQTAAEYNARFEQQKQLERQAILRDSLKIDELKKQIAELQ
                     AQLQTPQITEDQRQVTEQQTTTREAELESLREQLRESNRRLQELELSSLETTPPSMLR
                     VRNYGMWYAIIAAIVLLIILLIVLLVNHRKQVKRQELEWGYGSQIPMGPRPTQPILTR
                     QPEETPKPQSPVPPITQPIATPAPPVESAQPAQPSAPAAPSAPPVDFEAQKEEIKSMR
                     QSVISMSVGQPETALRIINAWISQDIQPGKSETES"
     assembly_gap    7222..7321
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7322..7781)
                     /locus_tag="PLI55_01810"
     CDS             complement(<7322..7781)
                     /locus_tag="PLI55_01810"
                     /inference="COORDINATES: protein motif:HMM:NF025057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HPA99605.1"
                     /translation="MADEQNPRHKIFSHQDTGDEEPWLITYSDMMSLLLTFFILMFSI
                     STIDPVKLEQIGNSVGQALGGAKPKPTEHFSLKEIYDKVVDVIEQEQLKGVIEVETSS
                     RGVSIKIPSEISFASGKADLDPRIYPILNKLAPMIAQSKFPIAIEGHTDNV"
     gene            complement(7784..8578)
                     /locus_tag="PLI55_01815"
     CDS             complement(7784..8578)
                     /locus_tag="PLI55_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPA99606.1"
                     /translation="MDIATIFGIIAGTVLIIMGISGLTSDFKIFWDLSSVFIVVGGSF
                     ASTMISFSVKEVFSLIGSIIAVFKKEKIKMTDEVETIVELATVARKSVQDLEKALVDV
                     QNPFLRDGLQMVVDGYNPQEIRDILETRIEYRTLRERGEANVLRTMGKYSPAFGMIGT
                     LIGLVVMLYGMAQISSAGLDPMKVLGEGMGAAIITTFYGVILANLIFNPMAVKFETRI
                     QKQNILQMMLIEGILLLQARKHPLIIREKLNSYLRPRDWKNPEEIK"
     gene            complement(8587..9930)
                     /locus_tag="PLI55_01820"
     CDS             complement(8587..9930)
                     /locus_tag="PLI55_01820"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99607.1"
                     /translation="MTRPHLKISLLLLLTITFLQGESVTRLSFSHPGYMMRIPVSSVY
                     RTPYVFRTGFASDTYGFSDTLIAKGIFFEADLTNTFKVGLTTIQGISGTRPVEFGLHF
                     QKRVFTYGDISFTAGVHDMVLRQGEKNLNIDTETLSIFGLLSNEKQFTNNILHTYMGF
                     GTGGLASTFGTDSTANMGVFAGILVHTNIRKDQGGVSFMVEFDGGGVNLGTRIPLTQD
                     YNLSIGINDVTHLYNFGSKNYSTTPQNEHPSLSISLDFTIPRIKPENIKRRALEGMVD
                     PHTKAILDVEEQFAAKLDSTLKAADFEIAKLRDSLRVYETQIKYLTSQVAQLKQKSAV
                     LEDSVRSIKLAKYAMERNINLALKHLSNSLRYFYEGDYQQALKEVDAAIELNPNLALA
                     YARRGSIYYKMGDIERASINWNLALKIDPEYDDVRNILRAMSENRLRSVGEEQKE"
     gene            complement(9953..10678)
                     /locus_tag="PLI55_01825"
     CDS             complement(9953..10678)
                     /locus_tag="PLI55_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99608.1"
                     /translation="MKKFLIMITLPTLLSAQAFDSSRAVSTPNIMYDLGIFIEPARES
                     APLPKGVANPNLVPIQNQIDSLLSAFSIMNNQIRSLEQANDSLQKKVNELSKRIFLSP
                     FASLTVPAQIYSRHEGERFLQRGRAAYQANNYPVAIDYFLKVLASNMPDATIGDTYFW
                     IGYCYVQMQDEYLAIEYLKKVAEYPLSEKLDDALFLTGITYQKLGNPQMAEIFFSRLI
                     KRYPEGNLARLAALELKRLKGPD"
     gene            complement(10680..11459)
                     /locus_tag="PLI55_01830"
     CDS             complement(10680..11459)
                     /locus_tag="PLI55_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99609.1"
                     /translation="MPRSFNLSLLTVVITAVICFGQGTSSSSGTPIQDSNKTIDQKDI
                     EIYNLKEELEYLKKELRKLQEINTSLCDSLESKHTLLETTSEKNVDENYNLRIYHQTI
                     EDDRRLNQEIVKEIQSTAPIAVPFKSPEPSGDPEFRERYNEALNQYFDGNLSDSQKNF
                     KKLLELRQDHPLSDNCQYWLGECLYSQEKYEEAIAAFQTVKALGDGNKADAALFKIGL
                     CYLKLDKKAAASSAFNDLINFYPKSDLVPKAYQYLLSQDKF"
     gene            complement(11459..12406)
                     /locus_tag="PLI55_01835"
     CDS             complement(11459..12406)
                     /locus_tag="PLI55_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99610.1"
                     /translation="MKAKNFYVSIILAISLSACGTSRAKPEKTGEETTKAKSEKAENL
                     PESQSSKSTSSGPQPKIATAPVKATAADQSRLINELTFEVKKLSAEVKHLQSALKELQ
                     AKSQMWDNPLSIYDKEIIMANGTSIFGKIIYQDEKMIKVETWIGYLIIDKNQVVRVVT
                     NIVEKPDEQYVPPELASDIKDDKPITIQAPYISKTGEPAPIGEPTSRVPNCVLVGNIK
                     ERKDLSGNTILSGEIKNIGGRRADFVKINIVFRKNWSGDTETRTAFVEGSYVTFEGTG
                     ITSNNSLLPGATGTFEMIIPKDFGQFIGYTYNIDWEVYR"
     gene            complement(12627..13769)
                     /locus_tag="PLI55_01840"
     CDS             complement(12627..13769)
                     /locus_tag="PLI55_01840"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99611.1"
                     /translation="MIREQILKDLIHLPQNKFPVTSAYFTLDNIAGNRKSHIVEIKKQ
                     IRYKKEKTYFRQLSTEEQESVLSDFDRILHWFGKELNPAESISSICFSSSGANLWRTL
                     NFKKPLPQNILVIQPYPYIRPYTILFSRYRNYAVVLVDRSKAKIFESHLGEFREHFHI
                     EDNAPESVKVGGFKGRQERKVERNIHQGVIQHYKEVAQEIFDLHQKFDFHWIILGGRK
                     EVLAEFSKYLHPYISARIAGQIEVEPTAPLAEVLKKVEKTEEQARNNYEQALLKSLLD
                     RKSAGQTVDGIQAVLDKIQDNWVDKLIIQEHFFKKGLFCRNCGFLSLKPLASCPAGCG
                     KLERTNDLVEHLVHRALKQGVDLQFVSSNMDDYAGIAAILRFSIGG"
     gene            complement(13996..>14294)
                     /locus_tag="PLI55_01845"
     CDS             complement(13996..>14294)
                     /locus_tag="PLI55_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPA99612.1"
                     /translation="LLEKGINLCLGTESRVLVPDLDIRKEMVECVNTYGVSPELALKF
                     ATLNGAYAIGFHKEVGSLEKGKIARCLVVDCSGLTTTDPYEMILSTPHSPRWLI"
     assembly_gap    14295..14394
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14395..15365)
                     /locus_tag="PLI55_01850"
     CDS             complement(<14395..15365)
                     /locus_tag="PLI55_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPA99613.1"
                     /translation="MVNNQPENPEILKGYTAHWIVPVSQPPIEKGVIIVQGQQIIEVE
                     SADNLQTNIETPLVDLGDSVIFPGFINAHTHLEHPPLAETPVNFRKYMQFVCDFTRTD
                     EITKAEYAAANLAENINFGTVALADFTSKGASYSALMNNPIFARQFFEVTGFKNYQAS
                     EIIRTFRELIQDQTPEKKITKHLTPSYVWATSPQLLHEIAFTERHIAIHIDMIPEENE
                     FTLNGSGLLRQILLANEDFDYSWQVPGLTAIRYFFANHFYARHNILTHMNCVTGDEID
                     FIKEFGVKVNVCLCPRSSKILSQIKTPVDLLLEKGINLCLGTESRVLV"
     gene            complement(15460..17823)
                     /locus_tag="PLI55_01855"
     CDS             complement(15460..17823)
                     /locus_tag="PLI55_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPA99614.1"
                     /translation="MIKYILERMPLVGICVVYLSGIVTDHTLHLLPEVWFIFLLIDFL
                     IGIIFSSKARSVLLIALALFCLGGMNHALRTVTGPDHLSQFYFSNEPDSVIAMVEQAE
                     FSGDGKLKIRLKNLQIKHSDWQKWNGKLILTVKEPENNVIYGDWMAFYTRLNQPATAR
                     NPGEFDYRRYLANHHIYATAYLDAGQTPTIIGRSRSLRRAANYSRIKVEELIDKSMSG
                     EQNAILKALIVGVRGEISDETNQAFVDTGVVHVLSVSGMHVVYVTMVIMLILGFLRIP
                     KKPKMVLAILGLGFYALMVDLRPSVNRAVIMAILTLIAQGWEKRANVYNTIAAAALIQ
                     SLFDPLQIFDMGFQLSFMAVISIVYVYRQLEYLLPLPLKTSNIKNRRLRETWQLFLVS
                     LSALLGTIPITVFYFQRIPLISLVSNLAVVPLVGLIGGLGFGQVILGFLWSGFNLTFG
                     EVQTLLISLLTQIVKISGRVPIAYLTIPAISLAGVYFGYLILLILLNLDKKRVQKVAL
                     FGFLIGINFLIWTQNLSKPVLQVTFLDVGQGDAIFLEFPNRETMLIDTGDRTFRRDYG
                     ELTIAPFLKRKGFQRIDYLALSHPHNDHIGGAPYLMRNFQIGEIWEPDISASSQIYRE
                     IHTLADSLQIPIRHLYAGDCFPLAESLTVFVLHPSKAFLESHPPGFNDYSNVLKISYR
                     QIDFLFCGDVEKFSESYLNLWHATLQSEILKVPHHGSSTSSSQTFLEQITPDLAVISV
                     GKNNKFRHPSAGTIARYEALSTEIHRTDLSRALIINSDGHKYWIKPW"
     gene            complement(17858..18394)
                     /locus_tag="PLI55_01860"
     CDS             complement(17858..18394)
                     /locus_tag="PLI55_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99615.1"
                     /translation="MNDHTEFEEINLARHIYRYLITIGIALFGLFLISIEPNVLRQYR
                     SWIPSQTFNIYYLTWFAVCTISAILCFFWGVLCQFLRGNNCLGNVLWYVGIFTITLFF
                     ATVFFQILKTDRSVGLAVTFGTLIISAMVTMAISYIFYLLNFNSRYHFWLFTLAIISA
                     TSAYNWLYLSVIGKIPAF"
     gene            complement(18420..18884)
                     /locus_tag="PLI55_01865"
     CDS             complement(18420..18884)
                     /locus_tag="PLI55_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99616.1"
                     /translation="MRVVRYWLTLICIGIALYTASPLLAQNPTDIDGTIWRRMKYDNK
                     YYWLLGYLQGLEKSDEIIQLCLAEQEKREFAFTVPFYVDQMRARISAYFPPHNIQDIT
                     QLIELLNTFYKDKYNQKIKIEAALRIVLARQKGQIEQSDFWLNEARRALIQK"
     gene            complement(18875..20533)
                     /locus_tag="PLI55_01870"
     CDS             complement(18875..20533)
                     /locus_tag="PLI55_01870"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPA99617.1"
                     /translation="MKQIIKIGLAQINPVLGDLPGNAKKITNFIKEADQQGIDLLIFP
                     ELAITGYLPQDLLLNRSFIDCNLQCLNKITAACRSSMVVILGYVARENGKLYNAAAVI
                     KSGQLIATCYKTLLPNYDVFDEHRYFTPANENLPVPIEIGGHSVRLGVEICEDLWDSN
                     SAIKVTDQLVAQGAELLVNLSASPFEYDKRDRRRQLVLDKVTKLRRPFVLVNMVGGRD
                     ELVFDGNSFALDASGALIGWGGEFKENLAVFELDLVSGQGHRMPWPETQREASIYQAL
                     TLGVHDYFYKTGGRQAIIGLSGGIDSALVACIAVSALGAENVLGVFLPSQYTSAASRE
                     DSVVLAKNLNIRLIEIPIEKIFQSYLQELKPVFGQTSPDVTEENIQSRIRGNLLMALA
                     NKYQAYVLNTGNKTELALGYCTMYGDMCGALAVISDLSKLDVYKIAGYFNETAERLII
                     PERIFTKIPTAELAPGQVDPFDYTIVSPLVDLIINDQKTPAELIQMGYAADLVTDIFE
                     RIKFAEFKRRQAAPGLKITSKAFGLGRRYPIINHFKEKGGKQCE"
     gene            complement(20530..20805)
                     /locus_tag="PLI55_01875"
     CDS             complement(20530..20805)
                     /locus_tag="PLI55_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPA99618.1"
                     /translation="MAIRVAIIVQGVVQGVGFRWYVRRIAMRYRLTGYVKNLPDGDVY
                     CEVEGEEGMINDFISELKAGPPLSHVTNVIVNKLPDLVGYKSFEIRF"
     gene            complement(20810..21265)
                     /locus_tag="PLI55_01880"
     CDS             complement(20810..21265)
                     /locus_tag="PLI55_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HPA99619.1"
                     /translation="MTVSPKIIAVLGGRECDEQTYQLAFDVGRAIAERGAILLCGGRT
                     GVMEAACKGAVSAGGLTIGILPGADKNDANPWVKIALPTGIGVARNAIIARACDAAIA
                     IGGKYGTLSEIAYCLQLNKPICGLNSWPEISDLRQFNSASAAVEFAFGE"
     gene            complement(21265..>22188)
                     /gene="asnS"
                     /locus_tag="PLI55_01885"
     CDS             complement(21265..>22188)
                     /gene="asnS"
                     /locus_tag="PLI55_01885"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPA99620.1"
                     /translation="RKQWAILRLRHAVYYGITQYLNENGFIRFDSPILTPNACEGTTT
                     LFEVPYFDLGKAYLSQSGQLYLETGIMSLGRVYDFGPVFRAEKSKTRKHLTEFWMMDA
                     EAAFVEHEENMRIQEELIRFVIRKILAECQPELEILERNIEQLRKADAPFKKMTHREA
                     VEYLRSKGSSMTETEDLGAQDETLLTEGSDVPIFVEKWPKEIKAFYMKRDSENPDLVL
                     GDDLIAPEGYGEIIGGSQREDDYDLLLQRMIAENMPLDEYQWFLDLRKYGSVPHSGFG
                     VGLERLITWIAGVPHIRETIPFPRTIYRLRP"
     assembly_gap    22189..22288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22289..22715)
                     /locus_tag="PLI55_01890"
     CDS             complement(<22289..22715)
                     /locus_tag="PLI55_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPA99621.1"
                     /translation="MQSVFIKDIQNYNNQEVTLKGWVYNLRSSGKIWFLLLRDGTGLI
                     QGIVSRNDVSEKIFNYKEILTQESSVSVSGLIHEDKRAPGGFEIIVKDIQVYQIAKDY
                     PITLKEHGIEFLMDNRHLWLRSRKQWAILRLRHAVYYGIT"
     gene            complement(22773..23300)
                     /locus_tag="PLI55_01895"
     CDS             complement(22773..23300)
                     /locus_tag="PLI55_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPA99622.1"
                     /translation="MLFKRLAPIIKFTPAVLLCLIINSCWLYSFKGTLPPGIKSIAIP
                     LFEDRTAEFNIQQIVTDKIRLGFIKENLLKVVEEDNAHSVLYGTILSIDDKPLVYDED
                     ENVGESVTEYRVTIKIEIEWYDKVNDRTVFKKQFIGYSEYDPTGATSRTREVALSEAI
                     NQITEDIMNTILADW"
     gene            complement(23306..24529)
                     /locus_tag="PLI55_01900"
     CDS             complement(23306..24529)
                     /locus_tag="PLI55_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA99623.1"
                     /translation="MDNERINKLQQSFGILGESDYIKEIVETIDQVARTDITVLICGE
                     SGTGKEVVANAIYRHSLRKHQPFVKVNCGAIPAGILESELFGHVKGSFTGAIEDRKGY
                     FETANHGTIFLDEIGEMPPETQVKLLRVLELGEFLPVGGSEVRHVDIRVIAATNKELA
                     TEIERGNFRKDLYYRIKTITIYMPPLRKHPEDIPLLVEKFALDFANRNSIAFKGFSPT
                     ALNLLREYSWPGNIRELKNFVESMIVLEKGEVITSSMVEKHLLGEPRHIPSPHLPMKI
                     SKSVDQAERELILQQIMVLRQELHEIYEAVITNPLPLKYEALRELEIPPSLPRPKVEE
                     KEIGKLDNAEIINDEIINPEMVGKVTLAEVEKELIQRTLKKFNYRKRQAANALQISER
                     TLYRKINEYGLEESR"
     gene            complement(24545..25840)
                     /gene="purD"
                     /locus_tag="PLI55_01905"
     CDS             complement(24545..25840)
                     /gene="purD"
                     /locus_tag="PLI55_01905"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPA99624.1"
                     /translation="MKVLIVGSGGREHALAWKLSQSPYIAKIYCAPGNAGTALLAENI
                     PLKDNDLDGLLKFALENSIDLTVVGPEIPLVNGIVDLFRAQGLKIFGPTSAAARLEGS
                     KVFAKQFMSRHQIPTAAYQVCDSRAAMESATHFKTFPYVIKVDGLAAGKGALIIHNDD
                     DLREAFVAIWDAKRFGTAGNKVVVEDFLLGEELSVFVITDGEHYLILPPAQDHKRIGE
                     NDTGPNTGGMGAYAPAPLGTPQVMHNVEKQIIIPTLNGLKAEGIPYTGVLYCGLMING
                     TQPYVVEFNARFGDPETQVILPLIESDLGVILHSAVEGKLDATMMKLSRKTAVCVVMA
                     SAGYPGDYEKGKVIYGLQDEKPESDTIVFQAGTIRNNAHFLTNGGRVLAVTALADNLE
                     KAIARAYERVAEITFVGAYYRRDIGQKGLLRQKDLLEKI"
     gene            complement(26011..28023)
                     /gene="uvrB"
                     /locus_tag="PLI55_01910"
     CDS             complement(26011..28023)
                     /gene="uvrB"
                     /locus_tag="PLI55_01910"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPA99625.1"
                     /translation="MNKFKLTTNFTPTGDQPQAIRELTEGILRGDKFQTLLGVTGSGK
                     TFTMAQVIQNVQKPTLVISHNKTLAAQLYGEFKAFFPENAVEYFISFYDYYQPEAYLP
                     VTDTYIEKDSSINDEIDKLRLKATSSLISRRDVIVVASVSCIYGIGSPEEYTRFIVTL
                     KKGDRIGIRQLFGRLVDIYYARNDLVLERGKFRARGDIVEIRPAYEDYAYRIELFGDE
                     IEHIYICDPLTGEVTNEQEMVVIFPAKHFISSEETIQRAIVSIEEELEARLEELRSAG
                     KLLEAQRLGQRTRFDLEMIQEVGYCSGIENYSRHFSGRKPGQQPYTLLDFFPRDFLLI
                     IDESHVTLPQLRAMYNGDRSRKEVLVEYGFRLPSALDNRPLKFHEFEEMVNQAVFVSA
                     TPSDYELEKCNGVVVEQIIRPTGLLDPEIIVKPTKHQIDDLIDEIAAVTARKERVLVT
                     TLTKRMAEDLSEYLRGMGLRVRYLHSEIKALDRVSILRDLRLGNFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSEKSLMQVSGRAARNINGRVIFYADEITESMRKVIEEV
                     SRRRKVQQDYNIAHNIQPHTIYKSVAEVMQTTAVADVKPTPIQTDQPVGDRYRAKMET
                     SEIIELLRREMLAAAENLEFEKAAELRDEIARLTGQKKSLSRENLL"
     gene            complement(28162..28815)
                     /locus_tag="PLI55_01915"
     CDS             complement(28162..28815)
                     /locus_tag="PLI55_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99626.1"
                     /translation="MNKHFLIWAIGLWLSACATHPTISPVPLKPGETYTGVTFSVENV
                     APIFVYRRGVSERSDIGFRVGLPVYGSGIDFSYLLFHHDNIADVLNLSFSVNPNSNLD
                     MTYYSVRTFPSKPGNALYTGFRLMYIPYGITGGNSLRVGFLGGLSFSKKIGFEIGYFH
                     DFDKGQPIEHIFDWDSYNPERYPATTEYGFPTEYSRLTGISCQLSLSMQIFGKKSAK"
     assembly_gap    29046..29145
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <29146..30683
                     /locus_tag="PLI55_01920"
     CDS             <29146..30683
                     /locus_tag="PLI55_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007086659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPA99627.1"
                     /translation="EEQFSCFRRNIIGIDYQYNFPGGRRPILYADWAASGRLYRPIEE
                     YITNVLGPYVANTHSDATLTGTIITDAYHQAHKIIKQHVNAGPDDVLLLAGFGMTAVI
                     NKFQRLLGLRVPDKFKEKISIPPQERALVLLTHMEHHSNQTTWEECCCDTSLICKKSD
                     GLPNLEHLRNILELNRDRKLIIGSFTACSNVTGLITPVNELAEIMHQFGRLCFIDYSA
                     SAPYVEINMHPPKPEQKLDAIFFSPHKFLGGPGSSGVIVFDKKLYNNRVPDQPGGGTV
                     LWTNPWGEHRFFQDIEVREDGGTPGFLQAIRAALAILLKEEMGVENILAQEHYLTEIL
                     LNELAGIPDINILESQNHNRIGFVSFYSLKVHHNLIVRLLNDRFGIQTRGGCLCAGTY
                     GHVLLHIDDQRSKAIMSQIDQGNLSLKPGWVRISLHPTMTEAEVRYIASAVREIMKNY
                     VEWGKDYHLNPETGDFDHLKQPKASIKLRETFKAALRIFLPVVILNAYICFSFGNQIA
                     ISL"
     gene            30749..31000
                     /locus_tag="PLI55_01925"
     CDS             30749..31000
                     /locus_tag="PLI55_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HPA99628.1"
                     /translation="MDKKVKLYALSTCIYCQNTKAFLKSLGIEYDSIDVDLLDKEQKL
                     AVIREIHKLTPTVAFPVIVIGDNVIVGYRPEEIKKALGL"
     gene            30997..31332
                     /locus_tag="PLI55_01930"
     CDS             30997..31332
                     /locus_tag="PLI55_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011368558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HPA99629.1"
                     /translation="MNTEQLFESLRKLQEPKGYFFNKDRQHVDRLLNSLLINKERYGY
                     MACPCRLAVGDRESDRDIICPCVYREPDVKEYGRCYCGLYVSADWNAGKIPDRYVPER
                     RPIEKILGL"
     gene            31440..32393
                     /locus_tag="PLI55_01935"
     CDS             31440..32393
                     /locus_tag="PLI55_01935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPA99630.1"
                     /translation="MEVKETLFNYVNPAIEYQILNGDSCYILQNLPDGKFDLIITSPP
                     YNIGKEYETKTCIEKYLETQEEIIRQLIRVLSKQGSLCWQVGNFVNKGEIFPLDIYYY
                     PIFKKYQLKLRNRIIWHFEHGLHASKRFSGRYETILWFTKTDNYIFNLDAVRIPAKYP
                     GKLHFKGPNKGKPSGNPLGKNPSDIWKIVVEDWDNEIWNVPNVKSNHPEKTEHPCQFP
                     VELVERCVLALTNKSSWVLDPFAGVGSTIIASIKNERNCIGIEKEAHYCKIAQNRIEA
                     LKAGNLKVRPINKPIHKPSKNDKVANVPNEWLKLKFNGYSK"
     gene            32390..33004
                     /locus_tag="PLI55_01940"
     CDS             32390..33004
                     /locus_tag="PLI55_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015106973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HPA99631.1"
                     /translation="MKIIKKYSHLNGEEYLIVHHKDLYNEIKSVIKNINAEGLKTKVS
                     KENRKAGTQLYSSIELKKAFDREFSKRNWNESRYNYYITLNRELMEKSLLMPAGEQKK
                     FLIEHGEKNPIFSYNQTDFVKNKVAVEVQFGKYAFVAYDLFVKHMLFYSGGMINLGIE
                     ILPTKNMQSQMSSGIAYYEGEVYNVMRQGRNSPPVPLLILGIEP"
     gene            33145..33588
                     /locus_tag="PLI55_01945"
     CDS             33145..33588
                     /locus_tag="PLI55_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99632.1"
                     /translation="MDLNFLERFPLLYDWLKAHTTLLKYAGLISLITFFGTILLMGVL
                     ILMIPTDYFSNPKRRKSFLITKRPLLGVILLIVKNLIGLIFLIVGFLLLFIPGQGILT
                     ILVGIILMNFPGKYRLERKLVQRPGVLQGINKIRKKFNRPLLGFE"
     gene            33653..>34041
                     /locus_tag="PLI55_01950"
     CDS             33653..>34041
                     /locus_tag="PLI55_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99633.1"
                     /translation="MLKQFKVLLIWGILIVGFMACNDNGSEPPTAATLSLLRTEYGGC
                     NEYKGGDILPKAAIQNDSLSWEISDDTLKIFTGITYICCAPFDVESEQSGDSLKIMIR
                     DVCGISSEHCYCRCICHYTFLTIFTGYS"
BASE COUNT         9278 a         6966 c         7589 g         9808 t
ORIGIN      
        1 aacgaatttc cgcgaaccgt gcgctgtgtt ctcatcggcg ataactaccg gatttacgaa
       61 cgattgctga atcaaatgcg ttcgtcaact gtaaggtaat tccacgagta caattgttca
      121 atttttgggg tgaattattg gattcgggga tttttttttg taatctttgg cgtttaatga
      181 caggagaaaa ttaaaatgtt accttcagtc aatccgacta ccacttctgc ctggcaaaag
      241 ctgtcagctc attatcaaga aataaaaaaa atatcactgc gaaatttatt caatgaagac
      301 ccggagcggt ttcagcgttt ttcaattaat ttagttctcg atgaaacgag tcgagatgaa
      361 attctgctcg attatagtaa aaatctaatt acacctgaaa cttttcgact tttactcaaa
      421 ctggcgcgag aatgcggcgt cgccgaggcg atcgaattga tgttcaccgg ggcggcgatt
      481 aatgtcaccg aaaatcgtgc ggtcctccac acggcattgc gaaatctcga tgataatccg
      541 atttacgtcg atggtcagaa tgtaatgccg ccaataaagg ctgtccagga acaaatgcgt
      601 gaattcagtg ccaaagttat caatggcgaa tggcgtggtt acgatggtca gccgatcaaa
      661 gacgtggtta atattggaat tggcggcagt gatctgggac cggcgatggt gaccgaagcc
      721 ctaaaacctt atcacattcc gcatctaaaa ttgcattttg tttccaacgt agatggcacc
      781 gacatctgtg aaactttgaa atgtctaaac ccggcaacta cgctttttat cattgcctcc
      841 aagactttta cgacgcagga aacgatgact aatgcccaga cggcgcgcaa ttggtttcta
      901 accacggtcg gtgagcctga tttcatcaaa tcgcactttg tagcggtttc gactaatcgg
      961 gcaaaagtaa tcgaatttgg aattgacccg gaaaatatgt tcgtgttctg ggattgggtc
     1021 ggcggtcgct attcgctctg gtcagcaatc ggtttatcaa ttacgctggc ggtcgggttt
     1081 gataatttta gggaattgct ggctggcgcc tatgctatgg ataaccattt tcgccggacg
     1141 ccttttgaaa aaaatattcc ggtgattctg gcattgctgg gaatttggta taacaatttt
     1201 ttcggagcgg aaacgctggc tattttgcct tatgatcaat atttaaggcg tctgccggcc
     1261 tatctccaac aggcggatat ggaaagtaac ggcaagcgag ttgatcgcaa cggaaaagtc
     1321 gtcaactatc agaccgggcc aattgtctgg ggcgaaccag gtacgaacgg acagcacgct
     1381 ttttatcaac ttattcatca agggaccaag cttattccct gcgattttct agcgccagcc
     1441 atttcgcata atccggttgg agaacatcat aatattctat tatcgaatta tttcgctcag
     1501 accgaagcct tgatgaacgg caaaagcgag gaagaagttg cccgggaatt gaaatccaaa
     1561 ggtttgactg agtctgaagt tcagcgccag ttgccctatc gtgtttttcc tggaaataag
     1621 ccgagcaatt caatcctttt taaaaaatta actccgcgaa cgctgggtgc tctaattgct
     1681 atgtacgagc ataagatttt cactcaggga ataatctgga atatttatag tttcgatcaa
     1741 tggggtgtcg aacttggcaa gcaactggct ggacggattc tgccggaact tgcgaatgat
     1801 cagccgatta ccagccacga tagctcaact aatggattga ttaatgcctg gaagaggctc
     1861 cgtaagtaat cgccttaatg aggatattta tgcaatggtt tggtgaatta agtcccgttt
     1921 ttcaagcatt tttggcaacc tgttttactt ggttggtgac ggctggcggc gctggattag
     1981 tgattttttt caaggaagtc agtcaacgcc tattagatgc cgcactgggt tttgccgccg
     2041 gggtgatgat tgccgccagc ttttggtcgc ttttggctcc ggcaatagaa atggctggtg
     2101 catcgggtag tccggcttgg attccggcgg cggtcggatt tttattaggt ggcgcttttt
     2161 tgaaatcggt tgatcgtctg cttccccatt tgcatttagg tttgccgatt gatcaggcgg
     2221 aaggtattaa aactaactgg caaaggacgg tgcttttagt gctggcggtc acgatccaca
     2281 acgttccgga gggtttggca gtaggagtgg cttttggtgc ggcgggagcc ggcttgcctt
     2341 cggcttcact agctggtgca gttgccctgg ctctgggcat tggcattcaa aatttcccag
     2401 aaggtgtggc ggttacggtt ccactgcgtc gggagggact ttcgatcggc aagagttttt
     2461 tttacggtca actttccggt tttgtggaag tcatttccgg agttatcggt gcttatctgg
     2521 ttttgacgat tcaggctttg ttgccctatg ccctgtcgtt tgcggcgggg gcgatgattt
     2581 atgtcgttat cgaagaatta attcccgaat ctcagaaaga cgttagtacc gatgtcgcga
     2641 ctctgggagc gatgctgggt tttaccatta tgatgatttt agatgtagcc ttaggctaaa
     2701 atcttcaaga ctacaacaat taaaaaacct tatagaaatt aacaagttat cgtaagttag
     2761 ttcatctata aatttgttgc attcataact ttataaaagt tatatgattg caaaatagag
     2821 aatcatccaa ttaattggga tttggactta gaatgtcgca aaggttgcga tagtctaatg
     2881 tgatttgtaa acggtttagt tttattcacg tcccatacga gcggcttttg cgcgaatctg
     2941 ggggatggct tctgtcataa tgctttttac gtcggcataa cggaaagtcc gctcctcaaa
     3001 agagcgcgtc tcgagggctt tcaggaaacg attttggaat tcgtcaaaat aagaaccgta
     3061 gaggtgatag ctatccgctt gatggacata gcgaccgatg ttgatttttt caccgattga
     3121 ttgctcgatt cgattagcga tttcgacctg gagctgggtt agcgcaaaca tattcataaa
     3181 ggcggctttg taagcatcgt tagagcgaaa gcggacattc gtattgagag tccagatcga
     3241 attatcgtct ttcagcaaac ggcaccaaat gctttgcaaa caaggcgggt cgtagcaatc
     3301 gttgtcttcc catactttcc aggtgattgc ttgggctcga cgcgtatgag ggactttgat
     3361 taattttgcg accataatcg caatctggtc ataaactttc gcgaaaccag gcacctgata
     3421 attgaataaa cgttggtgat aagtatattc ccagcgggca tcggcgggat tctcagccga
     3481 ccggatgcaa tggtctttga ttccttccaa cacttccaga acatattctt gcaggtctgt
     3541 caatccacct ggaaaatcac aatgaatcat tggttcggct aaagggtttt ggacgacgat
     3601 catcatagtg caatctttgc taggcggatc agaaggtttg tcgtactcgg ttttgatatc
     3661 gcaacctttt tggtatagtt cgactaatga tttttcccag gcggcggcga gcgattcgcc
     3721 gttaaccata agcacgggaa tttgaccgtt cataagttct taacctcatt ttgattattt
     3781 tcataatgta gcgcgtttgg caagaaggga ttgacattgt tttaaaaatt aaaaagccga
     3841 aatgaatata aagaaaaaat cggagtaatc atatcacgaa tccattaggc ctcaactaca
     3901 gcgttgattt accaataaat tatttgttcg ggaaaccttg tttagcttct aatataaggc
     3961 cgtttcagat attgctcagc caggctgacg gctgccgtgc gattattaag ctgggcggct
     4021 ttgcgataac aggcgatagc ctcggatcgg cgtttttgta gatcgtaaac attacccaat
     4081 ctcacgagag cattgccgag gtcataatcc agttcataat cgtaggattt gatgaattcg
     4141 atcaaattcg attcggcttc atcgagccga ccttgggcaa gcgccaaact gccatagaga
     4201 aggcggagta ttccttgata atccttttga gttctgggag gttgagttgg aagcaaagcc
     4261 tcaatccgtt ttaggtaata attagcctga tcgagctggc caatctggat cgcggcgtcg
     4321 gcggtttggg cggcataata aggattttta gggaaatctt cagccatttt gcgggcatat
     4381 tcataagcgc cgtcataatt cgattcaaaa tagaaataga ggtaagccag aagcgatcgg
     4441 gcttcatagc gcccgtagtt gccgtaatcg gcggcggctt tgatggcttg gaggccttca
     4501 ctgcgcgagc cagattcgcg cattaggcga ctggcaattt tcatataagg ggctgagatg
     4561 ctgacataat agttaaatag accgaccggc aattggaaat ccggttggtc aggatagagt
     4621 ttttcggtct ttttgataaa tcggatagca ttataaccgc tagttagcac gccgaagtaa
     4681 tttttttctg ccaattgaat gcgggcgcgc aagcccataa cagttccata gaaaaagaag
     4741 tattccgaat gtgatgggtt ttgtttaatc tgttccgaat aaatttgcaa agcctgttca
     4801 atatccacaa gaaattgttg attacgctgg tgatagccga ttaatccttc gttttgataa
     4861 tattcgaccg ccaatgctcc cagtggaccc ataggatgaa agggagtgga gtttaatact
     4921 tgatggaaaa gactggcggc gcgtttgtat tcccgattgt acattgcttt aagagcaatt
     4981 tgaactctct cttcctgttg ttcaaaagtg tcagatttca ggttggcaaa accaccgagt
     5041 agcagggtga ttaataaggt cttaataatt ttcataaggt agttgtttta aaatagatgg
     5101 tatagactat caaattagca ttaaaaaagc cccagcgcgg ggcttttttg gtatttatta
     5161 aaactattat tcaataaagt cggtatccat aaaatcttcg agattgaagc gtccttcttt
     5221 ttcgagttcg cgtaattttt cgacaatttt tttctgagcg gcttcgactt tacgccgtgg
     5281 ttgtggacct tctaatagct tcatttcatc ttccagaatg atctgggaac gttggggtag
     5341 taagctttta aagaggtcgc gtatttcatc cggctgacct tttaaagcat aagctatttc
     5401 ggaaatttcg accgatttta acacttcggc caggatgttt ggtggtaatt tgggaaggtc
     5461 ttcgaaactg aagtaatagc gttttatctg atcaaccagt tcgggtgctt ctttagataa
     5521 aaactcgagg aagcgttttt catcacgggg cggtatttta cttaaaatgc cagctacgtc
     5581 ttggccgccg ccgcgtttaa attcggaagc tcgtggaata aaagccgatt tgtccttaag
     5641 attattggcg atactgacca cgccttccag cggaatttcc gtgagatgcc ccatttcagt
     5701 taatactttg ggttggacgg tgggatcgag tttttgcaaa acgttgacct ggcgttcgac
     5761 cggcagctgg gcgattgcca gagccataat gattggtggc tcgctggcaa tcaaataggc
     5821 aatttgttcg tcggtcagtt tcaacataaa ctcaaatggc tttttggtct cctcctcggc
     5881 ggggataaat ttttcggaaa cgaagctgaa atagaattcc tcgaggacgg cttttttgac
     5941 atcgaacggg atattggcaa ttttggccat attttcacgg attttttgca tttggctacg
     6001 acttagtcct ttgaaaagct ggactgataa gtcgggacca agggctttaa agagaatagt
     6061 ggctttgtcc agtccactca agtttttggc gtctatcatt ttcttactcc tttaactctc
     6121 agtttcggat ttgcctggct gtatatcctg acttatccag gcattgatga ttcgtaaagc
     6181 tgtttcaggt tgaccaactg acattgaaat taccgattgc cgcatacttt ttatttcttc
     6241 tttctgggct tcaaagtcaa cgggtggtgc agatggtgcc gccggtgcag agggttgggc
     6301 gggttgagct gattctactg gtggagcagg tgttgcgatg ggttgagtaa tcgggggtac
     6361 tggcgattgg ggcttgggag tttcctctgg ctggcgggtt aaaattggct gagtcggacg
     6421 cggtcccatt gggatttggg aaccataacc ccactccagt tcctgtcttt tgacctgctt
     6481 gcgatgattg actaataaga cgattaggag aattatcagt agcactatcg ccgcgataat
     6541 cgcgtaccac ataccataat tgcgaactct taacatactg ggtggggtgg tttcgagaga
     6601 acttaattcc agttcttgca ggcggcgatt gctttctcgt aattgctcgc gcaggctttc
     6661 cagttcggct tcgcgggtag tagtttgttg ttcggtaacc tgacgctggt cttcggttat
     6721 ctggggagtt tgtaattgtg cctgtagctc agctatttgc tttttgagtt catcaatttt
     6781 taatgaatct cgcaaaatgg cttggcgttc gagttgcttt tgctgttcga agcgagcatt
     6841 atattcggcg gctgtttgac gacgttcgat gtcgtcaatt ttttcggcta tgtttttgag
     6901 gataatggtt tcggtcgcat ctttatcggg gctttttttg aaattggcgg tcataatact
     6961 gatgacatcg cctcgcgccc gatcataatg agcggcgacc gccacgacct gtctgacgtt
     7021 ttctatgatt tcaggagaga taccatcttc gagaatgaca ttgatttcct gacgttttat
     7081 aaccgggacg gatgtagcga cttttttttc ggtggtagaa gtcgatttag ccgtacctgt
     7141 agctggttgt ggtaaggcat cgagctgcgt ttgagactgt attcgttcct gggattgaat
     7201 tacagaagtt ggaatatcaa annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 ngaacattgt cagtatgtcc ttcgatagcg atgggaaatt tagactgcgc aatcattgga
     7381 gctaatttat tcaaaattgg gtaaatgcgt ggatctaagt ccgctttgcc agaggcgaag
     7441 gaaatttcac tggggatttt gattgaaacg ccgcgagaac tggtttccac ctcaatgact
     7501 ccttttaatt gttcttgttc gatgacatct acgaccttat catagatttc tttgagggaa
     7561 aaatgctcgg tgggtttagg tttggcgcct ccgagggctt gcccgaccga attaccgatc
     7621 tgttccagtt tgacagggtc aatagtggaa atagaaaaca ttaagatgaa aaaagtcagt
     7681 agtaatgaca tcatatctga ataggttatc agccacggct cttcatcgcc agtgtcctgg
     7741 tgagaaaata ttttgtgcct tggattctgc tcgtcagcca tcactattta atctcttcag
     7801 ggtttttcca atcacgcggt cgtaagtaag aattgagttt ttcacgaatg ataagcgggt
     7861 gtttgcgagc ctgcagtaat agtattcctt caattagcat catttgtagg atgttttgtt
     7921 tctgaatacg agtctcgaat ttgactgcca taggattaaa aattaagttg gctaatatta
     7981 caccataaaa agtagtaata atagcggcgc ccatgccttc gccgagcact ttcatcggat
     8041 ctaagccagc ggaactaatc tgagccattc catagagcat aactaccaac ccaatcagag
     8101 ttccgatcat tccaaaagcc ggtgaatatt ttcccatcgt gcgcaagacg ttggcttcgc
     8161 ctcgctcccg taaagtccga tattcgattc tggtttccag aatatcacgg atttcctggg
     8221 gattatagcc atctacaacc atttggagac catcgcgtaa aaacggattt tgaacatcaa
     8281 cgagtgcttt ttctaaatct tgtacacttt tgcgagccac agtagctaat tccacaatgg
     8341 tctcaacttc atcagtcatc ttgatttttt cttttttaaa tacggcgata attgaaccga
     8401 ttaaactaaa gacctccttt acagaaaagc tgatcatcgt cgaggcgaaa gagccgccta
     8461 ccacgataaa aacactggaa agatcccaga atattttgaa gtcactggtc aatccggaaa
     8521 tacccattat gattaatact gttccagcaa taattccaaa tatcgtggca atatccatac
     8581 tagtttttac tccttttgtt cttcaccgac actacgtaat ctattttcac tcatagcgcg
     8641 taatatatta cggacgtcat cgtattccgg atcaatttta agagcaagat tccagttaat
     8701 tgaagcccgc tcgatatcgc ccattttata gtagatcgaa ccacgtctgg cataagccag
     8761 agccagattg ggattcaatt cgatggcggc atcaacttcc tttaaggctt gctgataatc
     8821 gccttcgtaa aaatagcgga gagaattgga taagtgcttt aaagctagat taatattccg
     8881 ttccatagca tatttggcga gcttaatcga acgaaccgaa tcctctagaa cggcgctttt
     8941 ttgtttcaac tgggcaactt gactggtgag atatttgatt tgggtttcat atacccgaag
     9001 tgaatctcgt aatttggcga tttcgaagtc ggcggcttta agggtcgaat ccagcttggc
     9061 ggcgaattgt tcctcaacat cgagaattgc ctttgtgtga gggtcaacca ttccttcgag
     9121 agcacgccgc tttatatttt ccggtttgat tcgcggtatg gtaaaatcga ggctgatgct
     9181 aaggctggga tgctcatttt gaggtgtggt gctataattt ttactgccga aattatagag
     9241 atgagtaaca tcattgatgc cgatacttag gttataatct tgggttagcg gaatgcgtgt
     9301 gccaagatta actccaccgc catcaaattc caccataaaa gacacgccac cttgatcttt
     9361 tctgatattt gtatgaacaa gtattccggc gaaaacaccc atattggcgg tcgaatcagt
     9421 tccaaacgtg gacgccaaac cgcctgtacc aaatcccata taggtatgga gaatattatt
     9481 agtaaactgt ttttcattgc tcagcaggcc gaaaattgaa agtgtttcag tatcaatatt
     9541 gaggtttttt tcaccctggc ggagaaccat gtcatgcaca ccagccgtaa aactaatatc
     9601 gccataggta aaaacacgtt tctgaaaatg aagaccaaat tccaccggac gggtgccgga
     9661 aatgccctgg atagtagtaa gtccgacctt gaaggtattg gtgaggtctg cttcaaagaa
     9721 tattcctttg gcaattaatg tatccgagaa tccataggta tcgctggcga aaccggtacg
     9781 aaatacgtac ggtgttcgat aaacactact aaccggaatt ctcatcatat atccaggatg
     9841 ggaaaaagaa agacgtgtta ccgactcacc ttgtaagaat gttatggtta gcagtaaaag
     9901 tagtgaaatt tttagatgag gtctggtcat agagcgaagc tccgtttagt gttcaatcag
     9961 gaccctttag ccgttttaat tctaaggcag ctaatctggc gaggttgcct tcaggatatc
    10021 ttttgatgag tcggctgaaa aagatttcag ccatttgggg attgcctaac ttttgatagg
    10081 taattccggt caagaaaaga gcgtcgtcta gcttctctga taatgggtat tccgccactt
    10141 ttttcagata ttcgatagct agatattcat cttgcatctg aacgtaacaa tagccaatcc
    10201 agaagtaggt atcgccaata gttgcatcgg gcatgttgct ggctaatact tttagaaaat
    10261 aatcaattgc gaccgggtaa ttattcgcct gataggcggc tcgccctctt tgtaaaaaac
    10321 gttcaccctc gtgacgtgag tagatttgag cggggactgt caagctcgca aaaggcgaaa
    10381 ggaaaatgcg ctttgagagt tcattaactt ttttttgaag agaatcatta gcttgctcga
    10441 gcgatctgat ctgattattc attatcgaga aagccgaaag gagggagtct atttggttct
    10501 ggatgggaac caaatttgga ttagcaacgc cttttggaag gggggcagac tccctcgccg
    10561 gctctataaa tatccctaaa tcatacatta tattaggagt actaactgcg cgggatgaat
    10621 cgaacgcctg ggcagagagc agagttggta gagttatcat aattagaaat tttttcatgt
    10681 tagaatttat cttgagagag aagatattga taggctttgg gtaccaagtc actcttggga
    10741 taaaaattga tcagatcgtt aaaagccgag gaagctgcgg ctttcttgtc taattttaga
    10801 tagcataaac caatcttgaa gagagcggca tcggctttgt tgccgtcccc caatgctttt
    10861 accgtttgaa aagccgcaat tgcctcttcg tacttttcct gggaatagag gcactcgccc
    10921 agccaatact ggcagttatc ggataaggga tgatcctggc gcagttccag cagtttttta
    10981 aaatttttct gactgtcgct tagattaccg tcgaaatatt gattcaaggc ttcgttatat
    11041 cgttctcgga actccggatc accactcggc tcaggtgatt taaatggaac tgcgatgggg
    11101 gcggtggatt gaatttcttt aacaatctct tgattaagac gtcggtcatc ttcgatagtc
    11161 tggtgataaa tgcgcagatt gtaattttca tcaacatttt tttcagatgt ggtttcaaga
    11221 agagtatgtt tggattccag tgaatcgcat aaagaggtat tgatctcctg taattttctc
    11281 aattcttttt tgagatattc aagttcttcc tttagattgt aaatttcaat atctttctga
    11341 tcaatcgttt tattggaatc ctgaattggg gtaccggacg aagaactcgt accctgaccg
    11401 aagcaaatta cagccgtaat cacaaccgtt aaaagcgaaa ggttaaatga tcggggcatt
    11461 atctataaac ctcccaatca atattgtaag tataacctat aaattgtccg aaatccttag
    11521 ggatgatcat ctcgaaagtt ccggtggcac ccggcaacag ggaattatta ctcgtgattc
    11581 ccgtgccttc aaaagtaaca taagagcctt ccacaaaagc tgttcgggtt tcagtatcgc
    11641 cgctccagtt cttacgaaaa acgatattga ttttgacaaa atcagcccga cgacctccaa
    11701 tatttttaat ttctccggag agaatagtat tgccgcttag atccttgcgt tctttgatgt
    11761 tacccactaa tacacaatta ggaacacggc tggtcggttc accgattggc gctggttcac
    11821 cggtttttga tatataaggg gcttggatgg tgattggctt atcatcttta atatcgctgg
    11881 cgagttcggg cggcacatat tgttcgtctg gcttctcaac tatattagtc acaactctca
    11941 ccacctgatt tttatcgata atcagataac cgatccaagt ttcaacttta atcatttttt
    12001 cgtcttgata aatgattttg ccgaaaatcg aggtgccatt tgccatgata atttctttat
    12061 cgtaaatgga aagtggatta tcccacattt ggctcttagc ctgtaattct ttcagggctg
    12121 attggagatg ttttacctcg gcggatagtt tttttacttc gaaagtaagt tcattgatga
    12181 ggcggctttg gtcggcggcg gtagctttga ctggcgctgt ggctattttg ggctggggac
    12241 ctgaagacgt tgatttagaa ctttgcgact cgggtaaatt ttcagctttt tccgatttgg
    12301 ctttagttgt ttcttcgccg gtcttttctg gtttagctcg agaagtacca cacgcactaa
    12361 ggcttatcgc cagaatgata gatacataaa aatttttggc tttcataatc ccacaccctc
    12421 agataaacag tgcctatatt ttttaatttg acactatagc gttgcatcca gatagaatat
    12481 atattttaaa atggtaaaat gtcaagtaaa aaattgatgt aaataagcaa gttagctaaa
    12541 attacttaaa attttaattg aaatagccaa taattaagct ctctaaatta ttttttcaat
    12601 ctgagaatgg agatttatca tgaaatttaa ccaccgatag agaagcgtaa aatggccgca
    12661 atccccgcat aatcatccat attgctacta acgaactgga gatccactcc ctgtttcaaa
    12721 gctcggtgaa ccagatgttc aaccagatca ttggttcttt ctaattttcc gcatccagct
    12781 gggcaagagg caagtggttt taagctaaga aagccacaat tgcggcagaa taatcctttt
    12841 ttaaaaaaat gttcttggat gatgagttta tcaacccaat tgtcctggat tttatcgaga
    12901 acagcctgaa taccgtccac agtctggccg gctgactttc gatcgagtaa gctttttagg
    12961 agtgcttgtt cataattatt tcttgcttgt tcttcggttt tctctacttt tttcaggact
    13021 tctgccaagg gtgcggtcgg ttcgacttcg atctggccag ctattcgcgc actgatatat
    13081 gggtgcaaat attttgaaaa ttctgccagg acttcttttc tcccgccaag aattatccaa
    13141 tgaaaatcaa acttttgatg aaggtcgaaa atctcttgag ctacttcttt ataatgttga
    13201 atgacacctt gatgaatatt gcgctcgact tttcgttcct gacgtccttt aaagccgccg
    13261 actttgactg actcgggggc gttgtcttcg atgtgaaaat gttccctgaa ctctccaaga
    13321 tgcgattcaa atattttagc tttggaacga tcaaccaaaa ctacggcata attgcggtag
    13381 cgactgaaaa gtatagtata gggtcgaatg tagggatacg gttggatcac gagaatattt
    13441 tggggcaaag gttttttaaa attgagtgtg cgccataaat ttgcgccact ggaggagaaa
    13501 cagattgaac tgatactctc cgctggattc aattcttttc cgaaccagtg taaaatacga
    13561 tcaaaatcgg atagtaccga ttcctgctcc tcagtactta attgccgaaa gtacgttttt
    13621 tctttcttat agcgaatttg cttctttatt tcaacgatat gactcttccg attgccggca
    13681 atattatcga gtgtaaagta agcgctggtt acgggaaatt tgttctgcgg gaggtgaatc
    13741 aaatctttta gaatctgctc tctaatcatg ttgactcctt tggttaagaa ttatatttta
    13801 cgcgacaaaa agccgtatcc attaattaat tgcactccga ttgtcatcgt tggtgcttat
    13861 tttttcttct tcagttaagt aggtttcagt gaccaattca gcgagactta gaaatcttaa
    13921 tctcacccta attaataagg aaaatttttg acattagcaa caagtattta tttttatctt
    13981 aagaaaatta ttttttcaga tcaaccagcg aggagaatgc ggtgtactta gaatcatttc
    14041 atacgggtca gtagtcgtca gaccgctaca atcaacgacc aaacaccgag caattttacc
    14101 tttttccaag ctaccgactt ctttgtgaaa tccaatagcg taggcaccgt tcagtgtcgc
    14161 aaatttcagg gcaagctcgg gggatacacc gtaggtgttt acacattcga ccatttcttt
    14221 gcgaatatcg aggtctggga ctaaaacccg actttcggtg ccgaggcata ggttaatccc
    14281 tttttccagt agtannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnactaaa
    14401 acccgacttt cggtgccgag gcataggtta atcccttttt ccagtagtaa atcaactggt
    14461 gtcttgattt gcgataagat tttgctactg cgtgggcaaa ggcagacatt gacttttacc
    14521 ccaaattcct tgatgaagtc gatttcatcg ccggtgacgc aattcatatg ggttagtata
    14581 ttgtgccgag cgtagaaatg attagcaaaa aaatagcgaa tagccgttaa acccggtacc
    14641 tgccagctat aatcaaaatc ctcgttggcg agtaaaatct gccttaataa accgctaccg
    14701 ttgagagtaa actcgttttc ttctgggatc atatcaatgt ggatagcaat atgccgttcg
    14761 gtgaatgcga tctcatgtaa taattggggc gaagtcgccc aaacgtaaga tggggtgaga
    14821 tgtttggtaa tttttttctc aggggtttga tcctggatta gctcacggaa agttcgaatt
    14881 atctcggaag cctggtagtt tttgaaaccg gttacctcga agaattgacg agcaaagatg
    14941 ggattattca ttaaggctga gtaagaagcg cctttggagg taaaatcggc taatgcaacc
    15001 gttccgaagt tgatattttc tgccagattg gcggccgcat attccgcttt agtgatttcg
    15061 tcggtccgtg taaaatcaca gacgaattgc atatatttgc gaaaattaac cggcgtttcg
    15121 gccaggggtg gatgttcaag atgagtatgg gcgttgataa atcccggaaa tatgacgcta
    15181 tctccgagat cgactaaagg agtttcgata ttagtttgca gattgtctgc gctctcgacc
    15241 tcgattattt gttgaccttg aactattatg actccctttt caataggcgg ctgagaaacg
    15301 ggaacgatcc agtgcgccgt atagcctttc aggatttccg gattttccgg ttgattgttc
    15361 accatattta cggttactcc gtgatcaaat tttaataatt aaactaaaac aattttatcg
    15421 aatagcgtac taaaaaccaa acctaagacc gaaataaatt caccatggct tgatccagta
    15481 tttatgaccg tccgaattaa tgataagggc gcggctcagg tcagtgcggt gaatttctgt
    15541 gctgagtgct tcataacggg caatggtacc agccgacgga tgcctgaatt tgttattttt
    15601 tccgacggat attactgcca aatccggagt aatctgctcg agaaatgttt gacttgaaga
    15661 agtcgaactg ccgtgatgtg ggaccttcaa tatttctgat tgcaaggtcg catgccatag
    15721 atttagataa ctctcggaaa acttttcgac atcgccgcag aagaggaagt caatctggcg
    15781 atagctgatt tttagaacat ttgagtaatc gttgaatccg ggaggatgcg actcaaggaa
    15841 cgcttttgac gggtgcagaa cgaagacagt cagtgactcg gctaagggaa aacaatcccc
    15901 ggcgtagagg tgtctgattg gaatttgcaa ggaatcagcg agggtatgaa tttcgcgata
    15961 aatctgagaa ctggcggata tgtccggttc ccagatttcg ccgatttgaa aattgcgcat
    16021 aagataaggc gctccgccga tatggtcatt atgtgggtga ctcaaagcca gataatcaat
    16081 gcgctggaag cctttgcgct tgaggaatgg cgcgatcgtt aattctccgt aatcgcgtcg
    16141 gaaagtccga tcgccagtat caattaacat agtttcgcga ttggggaatt ccaaaaaaat
    16201 cgcgtcgccc tgaccgacgt ccagaaacgt tacctgaaga accggtttgg ataaattttg
    16261 cgtccagatt agaaaattaa tgccgataag aaagccaaat aaagctactt tttgaactcg
    16321 ttttttatcg aggtttagca gaattaaaag aattaaatat ccaaaataaa ccccggcaag
    16381 gcttatcgcc ggtatcgtca gataagcaat tggtactcgc ccggagattt tgacaatctg
    16441 agtcaacaag ctgattaaaa gcgtctgaac ttcgccgaaa gtcaaattaa atccgctcca
    16501 caaaaatccg aggattacct ggccgaaacc caaaccacct atgagtccga ccaatggtac
    16561 aactgctaaa ttgctcacca gcgaaatgag aggaatccgt tggaaataaa aaaccgtaat
    16621 cggtatggtg cctaatagag ccgaaagcga aacgagaaag agttgccagg tttcacgcaa
    16681 acggcgattt ttgatgttgg aagttttaag aggcagaggc aataaatatt ccaattgtcg
    16741 gtaaacatag acgatcgaaa ttaccgccat aaatgagagc tgaaaaccca tatcgaaaat
    16801 ctgaagtggg tcgaacaggg actgaatcaa agcggccgcg gcaatagtat tatagacatt
    16861 ggctcgcttt tcccagcctt gggcgattaa tgttaaaatt gccataataa ctgcccggtt
    16921 gacggacggt cgtaggtcaa ccatcaaggc atagaatccc aagccaagaa tcgctagaac
    16981 cattttcggt tttttaggaa tacgcagaaa gccgagaatc agcataatca ccatggtcac
    17041 ataaactacg tgcattccgg aaaccgatag aacatggaca acgccagtat caacgaatgc
    17101 ctgattagtt tcatcgctga tttcaccgcg tactccgacg atcaaagctt ttaaaatggc
    17161 attttgttcg cctgacatag atttatcaat aagctcctcg actttaatgc gagagtaatt
    17221 ggcggctctg cgcagggatc ggcttcgtcc aatgattgtc ggagtctgcc cggcatccaa
    17281 ataagccgtg gcgtaaatat gatgattagc cagatagcgc cggtaatcaa attcgcctgg
    17341 attgcgcgcc gtagctggtt gattaagccg ggtatagaat gccatccaat cgccgtaaat
    17401 gacattgttt tcaggttctt tgacggttag aattaacttg ccgttccatt tctgccagtc
    17461 ggagtgtttt atttgcagat tcttcaagcg aatttttaac ttgccgtcgc cgctaaattc
    17521 tgcctgttca accatagcga ttacactgtc cggttcatta gagaaataaa attgacttaa
    17581 gtgatcaggg ccagttacag tacgcagggc atgattcatt ccgccgagac aaaagagcgc
    17641 taaagcaatt aatagaaccg atcgagcttt tgatgagaaa ataattccaa tgaggaagtc
    17701 aattagcaaa aaaatgaacc agacttctgg taataggtgc aaggtatggt cggtaacaat
    17761 tccgcttaga taaactacac aaataccaac gagtggcatt cgctcaagaa tgtacttgat
    17821 catttccttc ctattttatg acaaacgacg atttaagtta aaacgccgga atcttgccaa
    17881 taacgctcaa gtataaccaa ttataagcgc tggtagccga aataatagcc aatgtgaaaa
    17941 gccaaaaatg ataacggctg ttgaaattca aaaggtagaa aatataggat atcgccattg
    18001 taaccatcgc ggaaattatt aatgttccaa aagttaccgc caatccaact gatctatcgg
    18061 tttttaaaat ctggaaaaat acggtggcga aaaagagtgt tatagtaaaa atgccaacat
    18121 accagaggac atttcccaga caattattgc ctcgtaaaaa ctggcagaga actccccaga
    18181 aaaagcaaag tatcgccgaa atggtacaaa ctgcgaacca ggtcagatag taaatattaa
    18241 aagtttggga gggaatccaa ctgcgatact ggcgcaggac attcggttcg atagaaatta
    18301 agaataatcc gaataaagca atgccgattg taatcaaata acgatagata tgccgcgcca
    18361 gatttatttc ttcaaattcg gtatggtcgt tcatcgtacc ttctccggga ttattggaat
    18421 tatttttgga tgagcgcgcg tcgggcttca ttcagccaga aatcagactg ttcaatctgt
    18481 cctttttggc gagctaggac aatgcggagc gcggcttcga tcttgatctt ctggttgtat
    18541 ttgtctttat aaaaagtatt cagcagttca attagttgag tgatgtcttg aatattatga
    18601 ggcggaaaat aagcactaat ccgggcgcgc atctgatcta cataaaaagg aacggtgaag
    18661 gcaaattcgc gtttttcctg ctcagcgaga caaagttgaa tgatttcgtc cgatttttcc
    18721 agtccttgca gataacccaa tagccagtaa tatttattat catatttcat ccgccgccaa
    18781 attgtgccgt caatgtcggt tggattttgg gcaagaaggg gtgaggcggt atacaaggct
    18841 attcctatac aaattaatgt cagccaatag cgaactactc gcactgcttt cctccctttt
    18901 ctttaaaatg attaataatt ggataacgcc gccctaaacc aaaagcctta ctggtgattt
    18961 tcagcccggg cgcggcttgg cggcgtttaa actcggcaaa tttaatgcgc tcaaaaatat
    19021 cggttaccag gtcagctgcg taacccatct gaattaattc cgctggagtc ttttgatcat
    19081 tgatgattaa atctaccaac gggctgacga tcgtgtaatc gaatgggtcc acctgtccgg
    19141 gggctaattc agctgtcggt attttggtga aaatacgttc cggaatgatt agccgttccg
    19201 ccgtttcatt gaaataacca gcgatcttat aaacatctaa tttgcttaaa tcggaaatca
    19261 ctgccagcgc gccgcacata tcgccgtaca ttgtacaata accgagtgcc agttcggttt
    19321 tattgccagt gttcaaaacg taagcctggt atttattcgc cagcgccatc agtaaattgc
    19381 cccgaatgcg cgattggata ttttcttcgg tgacatctgg tgacgtttgg ccgaaaaccg
    19441 gtttcaattc ctggagataa gactgaaata ttttttcgat tgggatttcg attaatctga
    19501 tattcaaatt tttagctaaa acaacagaat cctctctgct tgcggccgaa gtgtattgcg
    19561 acggcagaaa tactcctaga acgttctcag ccccgagagc ggatacggcg atgcaggcaa
    19621 caagcgccga atcaattccg ccactcaatc cgataatcgc ttgtcgccca ccggttttat
    19681 agaaataatc gtgcacgccg agggtcaaag cctgataaat gctggcttcg cgttgagttt
    19741 cgggccaggg cattcggtgg ccttgtccgc taaccaaatc cagctcgaat acggctaagt
    19801 tttctttgaa ctctccgccc caaccgatta acgccccgga ggcgtccagt gcgaaagaat
    19861 tgccatcgaa aaccagttcg tcacgaccgc caaccatatt gaccagtaca aacggtcgcc
    19921 tgagtttggt tactttgtcg agtactaatt gccgacggcg gtcgcgctta tcatattcaa
    19981 atggcgaagc cgataaattg accaataact cagcgccctg agcaactaat tgatcggtta
    20041 ctttgatagc gctgttacta tcccacaagt cctcgcagat ttcaacaccg agccggacag
    20101 aatgaccacc aatttcgatt ggtaccggca ggttttcatt agcgggtgtg aaatagcggt
    20161 gttcatcgaa aacatcataa ttgggcagaa gtgtcttata gcacgttgca atcaattgac
    20221 cggatttaat gacggcggcg gcattgtaaa gtttgccatt ttcccgagcg acgtagccca
    20281 gaatgacgac catcgaacta cggcaagcgg cagttatttt attcaaacat tgcaagttgc
    20341 aatcaataaa cgagcggttc agcaataaat cttgcggcaa atagccggtg attgccaatt
    20401 ccggaaaaat caataagtca attccctgtt gatcagcctc tttaataaag ttggtaattt
    20461 ttttagcatt gccgggtaaa tcaccgagaa cgggattaat ctgcgccagg ccgattttta
    20521 ttatttgttt caaaatctaa tctcgaaact tttatagccg accaagtcgg gtaatttatt
    20581 gacaattaca ttagtaacgt gacttaatgg tggtccggct ttgagttcgc tgataaagtc
    20641 attgatcatt ccttcctcgc cttcgacttc gcaataaacg tctccgtcgg gtaaattttt
    20701 gacataaccg gtcaagcggt agcgcatggc aatccgacgg acataccagc ggaaaccaac
    20761 tccctgaact acgccttgaa cgataatcgc aacgcgtatc gccatatatt tattccccga
    20821 aagcaaattc cacggcggcg gaagcgctat taaactggcg cagatcgcta atttccggcc
    20881 aggaatttaa tccgcaaatc ggtttattca attgcaagca ataggcaatt tccgacaagg
    20941 tgccatattt accaccaata gcaatggcgg catcacaagc acgcgcaatg atagcattgc
    21001 gtgcaacacc aattccggtt ggtagggcaa ttttcaccca cggattggcg tcatttttat
    21061 ctgcgccggg caaaatgcca atggtcaacc caccggctga aactgctcct ttgcaggcgg
    21121 cttccataac gccagtgcgt ccaccgcaga gtaaaatcgc acctcgttcg gcgatggcgc
    21181 gaccgacatc aaaagccaat tgataggttt gttcgtcgca ttcgcggcca cccagaacgg
    21241 cgatgatttt tggtgaaacg gtcatcatgg tctcagccga taaatcgttc tgggaaatgg
    21301 tatagtttcc cggatgtgtg gcaccccggc aatccaagtg atcaatcttt ccagtccaac
    21361 gccaaagccg ctgtgtggta ctgagccgta tttacgcagg tcgagaaacc actgatattc
    21421 gtcgagcggc atattctcgg caatcatacg ctggagcagt aaatcgtagt cgtcttccct
    21481 ttgcgagccg ccaataattt cgccataccc ttccggagcg atgaggtcgt cgccgagtac
    21541 gaggtcggga ttttctgagt cgcgtttcat atagaaggct ttgatttctt ttggccattt
    21601 ttcgacgaaa atcgggacat ctgacccttc ggtcagtaag gtttcatcct gagcgcccaa
    21661 atcttccgtt tcggtcattg atgagccttt gctacgtaaa tattcgactg cttcgcggtg
    21721 ggtcattttt ttgaaggggg catcggcttt gcgcaattgt tcgatatttc tctccaatat
    21781 ctcgagttca ggttgacatt ctgccagaat tttacggatt acgaaacgaa tgagttcttc
    21841 ctgaatgcgc atattttctt cgtgttcaac gaaggcggct tcggcgtcca tcatccagaa
    21901 ttcggttaga tgtttgcggg ttttagattt ttcagcacga aagaccggtc cgaagtcata
    21961 aacccgaccc agactcatta tcccggtttc cagataaagc tggccggatt gcgaaaggta
    22021 agctttgccg agatcgaaat aaggcacttc gaaaagcgta gtagtacctt cacaggcatt
    22081 gggtgtcaga atcggggagt cgaaacggat aaatccgttt tcattcaaat attgagtaat
    22141 tccatagtaa accgcgtggc gaaggcgcag aatcgcccac tgtttgcgnn nnnnnnnnnn
    22201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga gtaattccat agtaaaccgc gtggcgaagg
    22321 cgcagaatcg cccactgttt gcgcgaacgg agccacaggt ggcggttatc catcaaaaat
    22381 tcaatgccgt gttccttaag ggtaatcgga taatcttttg caatttgata gacctgaata
    22441 tctttgacga tgatttcaaa accgcccgga gcacgtttat cttcgtggat gagtccacta
    22501 acgctgacgg acgattcctg agtcaggatt tccttgtaat tgaaaatttt ttcggaaaca
    22561 tcattgcgtg atacgatacc ctgaatcaag ccggtgccgt cgcgcaagag taaaaaccag
    22621 attttaccgc tggaacgcag attataaacc cagcctttca aggttacttc ttgattgttg
    22681 taattttgaa tgtctttaat gaaaaccgat tgcatttttg cccctttcga gtccgatcag
    22741 gttttattta atttttataa ccgataaaat actcaccaat ctgccagaat tgtgttcata
    22801 atatcttctg taatttgatt aattgcttcg cttagagcca cctcacgcgt tcgggatgtg
    22861 gcgccggtcg ggtcgtattc actatagccg atgaactgtt ttttgaaaac tgtgcggtca
    22921 ttgactttat cgtaccactc gatctcgatc ttgattgtta cccgatattc ggtaacggat
    22981 tcgccgacgt tttcatcttc gtcataaacc agaggtttgt catcaatgct caagatggtt
    23041 ccgtatagga ctgagtgagc gttatcctct tcgacaactt tcagtaaatt ttctttgata
    23101 aaaccaagtc ggatcttatc cgttactatc tgttgaatat taaactcagc cgtgcggtcc
    23161 tcaaaaagcg ggatggcgat gcttttgatg ccgggcggca aggtgccttt aaatgaataa
    23221 agccagcagg agttgattat caaacataat aaaaccgctg gagtaaattt tatgatggga
    23281 gccaaacgct tgaatagcat tgcgattacc ttgattcttc cagtccgtat tcgttgattt
    23341 tacggtagag agtgcgttcg ctaatttgga gagcattggc ggcttggcgt ttgcgatagt
    23401 tgaatttttt gagagtgcgc tggattaatt ccttttcgac ttcagcgagg gttacttttc
    23461 ccaccatttc gggattgata atctcgtcgt taatgatttc ggcgttatct agtttcccga
    23521 tttctttttc ttcgactttg ggtcggggca gactgggtgg gatttccaat tcgcgcaagg
    23581 cttcatattt taatggaaga gggttggtaa tcacagcttc atagatttca tgcagttcct
    23641 gccggagtac cataatttgt tggagaatta actcacgctc ggcttgatcg acacttttac
    23701 taattttcat cggcagatga ggcgaaggga tgtggcgtgg ctcgcccagc aaatgcttct
    23761 cgaccatact actagtgatt acctcgcctt tttccagaac gatcatactc tcgacaaaat
    23821 ttttcaattc ccgaatattg cccggccagg aatattcgcg caataaattc agagcggtgg
    23881 gcgaaaaacc cttaaaagcg atcgaattgc gattagcgaa gtccagggca aatttttcaa
    23941 ctaaaagcgg gatgtcttcc ggatgtttac gtaagggcgg catataaatg gtgatagtct
    24001 ttatgcggta ataaaggtct ttgcgaaaat ttccacgctc gatctcagtg gcgagttctt
    24061 tattggttgc ggcgatgacc ctaatgtcta catggcgtac ttcggagccg cccaccggta
    24121 ggaattcgcc gagttccaat actcgcagaa gtttgacctg agtttctggc ggcatttcgc
    24181 caatttcatc gaggaaaatt gtgccgtgat tggcagtttc aaaatagcct ttgcggtctt
    24241 cgattgcgcc ggtgaaagaa cccttgacgt gaccaaagag ttcgctttcc agaatcccgg
    24301 ctggaattgc cccgcaattg actttgacga agggctgatg cttgcgcaag ctgtggcgat
    24361 aaatagcatt agcgacgacc tccttgccgg tgccgctttc gccgcaaatc aggacggtaa
    24421 tatcggtgcg cgctacttga tcaatagttt cgacgatttc cttaatgtaa tcggattcgc
    24481 ccagaatgcc gaaactctgc tgtagtttat taatgcgttc gttgtccatt ttttgcttct
    24541 tttcttaaat tttttccaat aaatcttttt ggcggagtaa tcctttttga ccaatgtcac
    24601 ggcggtagta agcgcccaca aaggtaattt cggcgactcg ctcataggct ctggcaatgg
    24661 ctttttcgag attatctgcc agagcagtca ctgctaatac ccgaccgccg ttggttagaa
    24721 agtgggcatt atttcggatt gttcccgcct gaaatacaat cgtatctgat tctggctttt
    24781 catcttgtaa gccatagata accttgcctt tttcatagtc gcccggataa ccggccgaag
    24841 ccataacaac acaaactgcc gtttttcggg ataatttcat catcgtggca tctaactttc
    24901 cttcaactgc gctgtgtaaa atcactccca gatccgattc aattaggggt aatatcacct
    24961 gggtctcagg atcgccaaag cgagcgttga attcgaccac ataaggctgg gtcccgttaa
    25021 tcatcaagcc gcaataaagt acaccggtat aaggaattcc ctcggctttt aaaccattca
    25081 aagttggaat gataatttgt ttttcgacat tgtgcattac ttgcggagtg ccaagcggcg
    25141 ccggagcgta agcacccata ccgccggtat taggtccggt atcattttcg ccgatgcgtt
    25201 tatgatcctg agctggtggt aggataagat agtgttcacc gtcagtgata acgaaaaccg
    25261 agagctcttc gccgagcagg aaatcttcta ctacgacttt atttccagcg gtaccaaaac
    25321 gcttcgcatc ccagattgcc acaaaagctt ctcttaagtc atcgtcgttg tgaattatta
    25381 gagcgccttt accagccgct aatccgtcaa ctttgataac atagggaaaa gtcttgaaat
    25441 gagttgcaga ttccattgcg gcgcgtgaat cgcagacttg gtaagcggca gtcggaattt
    25501 gatgacgact cataaattgc ttggcaaata ctttactgcc ctccagtcgg gcggcggcag
    25561 atgttgggcc gaaaatcttt aaaccttgag cacgaaatag atctactatg ccatttacca
    25621 gtgggatttc cggaccaact accgttaaat ctatagaatt ctcgagtgcg aattttagta
    25681 gcccgtccag atcgttatct ttaagaggaa tattttctgc caaaagggca gtcccggcgt
    25741 taccgggcgc acagtagatt ttagctatat atggtgactg tgacagcttc caagctaagg
    25801 catgttctct accaccactt ccgacgatta gaactttcat ttaattcatc ttaaagttta
    25861 ttaaaataaa aatgttaatc attttagagt aagtcaaaat gtcgtagtaa tctaccgagt
    25921 tttgattatt gaaacaagga aaaggttgga ttacataagc aattcattta aaaattttgt
    25981 acttgggcaa atcggtttag tcggaaaaat ctaaagcaga ttttcgcgag ataggctttt
    26041 cttctggccg gtgaggcggg cgatttcgtc ccgcagttcg gcggcttttt cgaattctag
    26101 gttttcagcg gcggcgagca tctcgcgacg aagtagttcg atgatctcag acgtttccat
    26161 tttagcgcgg tagcggtcgc caaccggctg gtcggtctgg attggagtcg gtttaacgtc
    26221 agccacggcg gtggtctgca ttacttctgc gacggattta taaatggtgt gaggctgaat
    26281 attgtgggcg atgttataat cctgttgaac cttgcggcgg cggctaactt cttcgatgac
    26341 tttgcgcata gattcggtga tttcatcggc gtaaaagata acccgaccgt tgatattgcg
    26401 cgccgcccga cccgaaactt gcatcagaga tttctcggaa cgcagaaatc cttctttatc
    26461 ggcgtctaat atcgcgacta atgatacttc cggcaagtcg agaccttccc gcaggagatt
    26521 aatgccgacc agcacatcga aatttcccag ccgcaagtcg cgcaaaatgc tgacacggtc
    26581 gagggcttta atctcggaat gcaaatagcg gactctaagt cccataccgc gcagatactc
    26641 agataaatcc tctgccattc tcttggtcaa cgtagtgacc agcacgcgtt ctttgcgagc
    26701 ggtgacagcc gcaatttcgt caattagatc gtcaatctga tgtttggtag gttttacgat
    26761 aatctccgga tcgagcaaac ctgtcggacg aataatctgc tccacgacta cgccgttgca
    26821 tttttccaat tcgtaatcgg agggagtggc agagacaaaa actgcctggt tgaccatctc
    26881 ttcaaattcg tggaatttga ggggacgatt gtccagagcc gacgggaggc gaaaaccata
    26941 ctcgaccagc acttccttcc gggatcggtc gccgttatac atagcgcgca attgaggtaa
    27001 agtgacgtgc gattcgtcaa ttattagtaa aaaatccctc gggaaaaagt ccagcagagt
    27061 ataaggttgc tgaccgggct tacgaccgga gaaatgccgc gaataatttt cgataccact
    27121 gcagtaaccg acttcctgaa tcatttcgag gtcgaagcga gtacgctgtc ccaagcgctg
    27181 ggcttctaat aatttaccgg cagaacgtaa ttcttcaagg cgcgcctcta gctcttcctc
    27241 gatagaaaca atggcacgct gaatcgtttc ttcggaagaa ataaaatgct tagccggaaa
    27301 gattacgacc atttcttgct cgttggtgac ttcgccggtc aacggatcgc agatataaat
    27361 atgctcgatc tcgtcgccaa atagttcgat acggtaggcg taatcctcat aagccggacg
    27421 aatctcgacg atgtcgccgc gagcgcgaaa ttttccgcgc tccaacacta aatcgttacg
    27481 agcataataa atatctacca gtcggccgaa aagctgacgg atgccaatcc ggtcgccttt
    27541 tttaagagta acaataaagc gagtgtactc ttccggcgag ccgataccgt aaatgcagga
    27601 aacgctggcg actacaataa cgtcgcgacg actaattaac gagctggtag ctttgaggcg
    27661 cagtttatca atttcatcgt tgatcgagga gtctttttcg atgtaagtat ccgtaaccgg
    27721 cagataagcc tctggttgat aataatcata gaacgaaata aaatactcga ccgcattttc
    27781 ggggaaaaac gccttaaact ctccatataa ttgcgcggca agtgttttat tgtgggaaat
    27841 gaccaaggtt ggcttttgca cgttctgaat gacctgtgcc atggtaaaag tcttaccact
    27901 tccggttacc ccgagcagag tctggaactt gtcgcctcgt aatatccctt cggtcagttc
    27961 ccgtatcgcc tgcggttgat cgccggtcgg tgtgaaattg gtggtgagtt taaacttatt
    28021 catagcatat caatttagga taaggaagaa ccagagtcaa ttaattttca gcggaatata
    28081 tcataaattt aattcataga tacattagaa acgtgaatac tgacttcatt aacaatcgaa
    28141 gttaaaattt aattattcgg tttattttgc ggatttcttt ccgaaaattt gcatacttaa
    28201 tgataattgg caggagattc cagtcaagcg cgaatattcc gtcggaaatc cgtactcggt
    28261 cgtagccggg tagcgttccg gattgtacga atcccagtca aaaatgtgct caatcggctg
    28321 tcctttgtca aagtcgtgga aataaccgat ctcaaagccg atttttttag aaaatgataa
    28381 tccgccaaga aatcccactc ttaggctatt tcccccggtg atgccgtagg gaatatacat
    28441 taaccgaaat ccagtgtata aagcattgcc gggtttggag ggaaatgtcc gtaccgaata
    28501 atacgtcata tcgagattgg aattaggatt tacggaaaaa ctcaaattaa gtacatcggc
    28561 aatattatcg tggtggaaaa gtaaatatga gaagtcaatg ccactaccgt acaccggcaa
    28621 cccgactcgg aaaccaatat cactccgctc tgagacgccg cgccgataaa cgaaaatagg
    28681 tgcgacattt tcaacagaga aagtcacccc ggtataagtt tcgcccggct tgagtggcac
    28741 cggactaatc gtgggatgag tagcacaggc gcttaaccag agaccgatcg cccatatcag
    28801 aaagtgttta ttcattttga ctacccttag attaaccaaa ttcaagcatt ttgtaaagaa
    28861 atatttgcta ttggtcataa taaaatggtg agggctttct tctgatgaac atttttattt
    28921 ttatcaaatt ctaagttcat aaatttacgc attgattttt gggcgaagat gatgtcaaat
    28981 aaaactggta ttgaagaaca atttagctgt tttcgccgta atattatcgg cattgattac
    29041 cagtannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgaa gaacaattta
    29161 gctgttttcg ccgtaatatt atcggcattg attaccagta taatttcccg ggcggtcgcc
    29221 gaccgattct gtatgccgat tgggcggcca gcggaagact ctaccgaccg atcgaggaat
    29281 atataactaa tgttttgggc ccttatgttg ccaatactca tagtgatgcg accttgactg
    29341 ggacaatcat aaccgatgcc taccatcaag cgcataagat tatcaaacag catgtgaacg
    29401 ccggtccgga cgacgtccta ttactggctg ggttcgggat gacggcggtt atcaataaat
    29461 tccaacgtct tttagggtta cgtgtacctg ataaattcaa ggaaaaaata tcaataccgc
    29521 ctcaagaaag agcgctggta cttctaacgc atatggagca tcactccaac caaaccacct
    29581 gggaagaatg ttgctgtgac acctcactca tttgtaaaaa atcggacgga ttaccgaatc
    29641 ttgagcattt gcgtaatatc ttagaactaa accgtgaccg gaaattaatt atcggctcat
    29701 tcaccgcctg ctcgaacgtt accggcttaa tcacaccggt taacgaatta gcagagatta
    29761 tgcaccaatt cggacgcctg tgttttattg attattccgc ttcagctccc tatgtcgaaa
    29821 tcaatatgca tccgcccaaa ccggaacaga aattagatgc gattttcttt tcaccgcata
    29881 aatttctggg cggaccgggc tcatcaggag taattgtttt cgataaaaag ctttataata
    29941 atcgcgttcc agatcaaccg ggcggcggca ccgtactttg gactaatccc tggggcgaac
    30001 atcgtttttt ccaggacatt gaagtgcgcg aagatggtgg aacacccgga tttttacagg
    30061 caattcgcgc cgcgctggca attttactga aagaagaaat gggcgttgaa aatattctgg
    30121 cacaggaaca ttacttgacc gaaatcctgc taaatgaact ggctggaatc cctgatataa
    30181 acattcttga atctcaaaac cataaccgca tcggttttgt ttcgttttat agtctgaaag
    30241 ttcatcataa tctgatcgta cgcctactta atgatcgctt cggtatccaa acacgtggcg
    30301 gctgtctctg cgctggaact tacggtcatg ttttactaca tattgacgac caacgatcca
    30361 aagcaattat gagtcagatt gatcagggca acttgagtct gaagcccggc tgggtgcgca
    30421 tttcgttaca tccgactatg actgaggccg aagtgcgtta tatcgcatct gcggtacggg
    30481 aaattatgaa aaattatgtt gagtggggaa aagattacca ccttaaccct gagaccggcg
    30541 acttcgatca tctcaaacaa ccgaaagcca gtatcaaatt gagagaaact ttcaaagccg
    30601 ctttaagaat atttttaccg gttgtaattt taaacgctta tatttgtttt agttttggta
    30661 atcagattgc aatatcattg tgaaattatc tcaaaagcac cgaaataaaa tttatccgca
    30721 gaattagccc cgaagaaagg agtcgtttat ggataaaaag gtcaaacttt acgctctcag
    30781 cacctgcatt tattgccaga ataccaaagc cttcctcaag agtctgggaa tcgagtacga
    30841 tagtattgac gtagatttgt tagacaagga acaaaaactg gcagtcatta gggagattca
    30901 taaattaaca ccgacagttg cttttccagt aattgtcatc ggagacaacg ttattgtcgg
    30961 ctatcgtccg gaagaaatca aaaaggcact cggattatga acacagaaca gttatttgaa
    31021 tcgcttcgca aacttcagga gccgaaaggc tattttttca ataaagatcg tcagcacgtt
    31081 gaccgattgc tgaattcttt attgatcaac aaagaacgtt atggttatat ggcttgtcca
    31141 tgtcgtctgg cggtcggtga tcgcgaatcg gatcgtgata tcatttgccc atgcgtttac
    31201 cgcgaaccgg atgtgaagga atatgggcgt tgttactgcg gtttgtatgt ttccgccgat
    31261 tggaatgccg gtaaaattcc tgaccggtat gtaccggaac ggcggccgat cgaaaaaatt
    31321 ctgggattgt aaagcgttga tcattatcct ctgacctttt aaatttctga agattaatta
    31381 ttcgcctttt aggtaaattt tacgtaaaat aatcagagtt tttaaacaaa acgaataata
    31441 tggaagtaaa agaaactcta tttaattatg taaatcctgc gattgaatat caaattttaa
    31501 atggggattc ttgttatatc ctccaaaatt tgccagacgg gaaattcgat ttaataatca
    31561 cttctccccc ttataatatt ggtaaggaat atgaaaccaa aacctgtatc gagaaatatc
    31621 ttgaaacgca agaagaaatt attagacagc taatacgcgt tctttctaaa caggggagtc
    31681 tttgctggca agtaggaaat tttgtaaata agggagaaat atttccgctt gatatttact
    31741 attatccgat attcaagaaa tatcaattaa aacttcgcaa tcgtattatc tggcattttg
    31801 agcatggatt gcatgcttcc aaaagatttt caggaagata tgaaaccatt ctgtggttta
    31861 ccaaaaccga taattacatt tttaatcttg acgccgttag aattcctgca aaatatccgg
    31921 gcaagttaca ttttaaagga cctaacaaag gaaagccttc cggaaatcca cttgggaaaa
    31981 atccttcgga tatctggaaa attgttgtag aagattggga taatgaaata tggaatgtac
    32041 caaacgttaa atcgaaccat ccggagaaaa cagaacatcc ctgccaattt ccggttgaac
    32101 tagtcgagcg ctgtgtttta gccttaacta ataaaagtag ctgggttctt gatccatttg
    32161 ctggtgttgg ttcaacaatt atagcttcga ttaaaaacga gcgcaattgc atcggtattg
    32221 aaaaagaagc ccattattgt aaaatcgccc aaaatagaat cgaagctttg aaagcaggta
    32281 acttaaaagt gagaccgatt aataaaccta ttcacaaacc ttctaaaaat gataaagttg
    32341 cgaatgtgcc gaatgaatgg ctaaaattaa aatttaacgg atatagcaaa tgaaaataat
    32401 taaaaaatat tcgcatttga acggcgaaga atatttgatt gtgcatcata aagatcttta
    32461 taatgaaatc aaaagtgtta tcaagaacat aaatgccgag ggattaaaaa caaaagtcag
    32521 taaggaaaat agaaaagcgg ggacacagtt atatagttca atagaactaa aaaaagcatt
    32581 tgacagggaa ttttcaaaaa gaaattggaa cgagagtcgt tataattact atatcactct
    32641 aaatcgggaa ctgatggaaa aaagtttatt aatgcctgca ggtgaacaaa agaaatttct
    32701 catagaacac ggggagaaaa acccaatttt tagctataat cagacagatt tcgtcaaaaa
    32761 taaagttgct gttgaagtcc aatttggtaa atatgctttt gttgcatatg atttgtttgt
    32821 aaagcacatg ctattttatt caggcggcat gataaactta ggcatagaaa ttcttcctac
    32881 taagaatatg cagtcccaaa tgtcaagcgg aattgcatat tacgagggag aagtctataa
    32941 tgttatgaga caaggacgga acagccctcc tgtaccgctt cttattctcg gcattgaacc
    33001 ttagagatat aatcattaat aaaacaactc ggttaatgcg tatatctaat agggcaaact
    33061 gttcaataaa atttcatttt aaattatttt taacatgatg aaatctataa cgtcttttgt
    33121 tttccggtaa tccccgattg tcctatggac ctgaattttc tggaacgttt tccgctactt
    33181 tatgattggc ttaaagcaca taccactttg ttgaagtatg ccggtttgat ttctttgatc
    33241 acattttttg gtacaattct tttaatgggg gtactgattc tgatgattcc gacggattat
    33301 ttttccaatc ccaaacgccg taaaagcttc ttgattacca agcgaccgct cttgggtgta
    33361 atattactga tagtcaaaaa cctaatcgga cttattttcc tgatagtcgg atttttacta
    33421 ctgtttatcc cagggcaggg tatcttaacg attctggtcg gtataatttt aatgaatttt
    33481 cccggtaaat acaggctcga aagaaagtta gtccaaagac cgggcgtttt gcagggcatc
    33541 aataaaattc ggaaaaaatt taatcgccca ttactcggtt ttgaataaat aattgtttaa
    33601 atattttagg tttttgttta tctttgagca aagtcgcaat taggaggtga ctatgttaaa
    33661 acaattcaaa gtcctactaa tctggggaat attaattgtc ggatttatgg cttgtaatga
    33721 taatggcagt gaaccaccaa ctgccgccac tctttcgtta ttacgaaccg aatacggcgg
    33781 atgtaatgaa tacaaggggg gtgacatttt accaaaagcg gcgattcaaa atgattcact
    33841 aagctgggaa atcagcgacg atacgctaaa gatttttacc ggaattacct acatttgctg
    33901 tgcgccgttc gatgtcgaaa gtgagcagtc cggtgacagt ctcaagatta tgatcagaga
    33961 tgtctgtggt atttccagcg aacactgcta ttgcagatgt atttgtcatt atacctttct
    34021 gacaattttt actggttatt c
//
LOCUS       DAOOMU010000011        33421 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1089_length_33421_cov_19.564976, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000011 DAOOMU010000000
VERSION     DAOOMU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 33421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33421
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1089_length_33421_cov_19.564976"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..541
                     /locus_tag="PLI55_01955"
     CDS             224..541
                     /locus_tag="PLI55_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99634.1"
                     /translation="MEDKKVDFSNEESRKNFLITKFDDLFQGINNLYGKELMEELFHR
                     MEKTIAIFHQDVENLIKELKSSNSTGSTDQPIMGISDIPQTPAEPNPFSANEDNDWAK
                     HLG"
     gene            577..1080
                     /locus_tag="PLI55_01960"
     CDS             577..1080
                     /locus_tag="PLI55_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99635.1"
                     /translation="MKELIKTIALVLVVVAVVYFFFEFEKVNKRLDILNARDSTHSVT
                     INDFDHNLHDLSLEFVGRGKHIQQFQQSLKDMNSRLDSVDLALSDKIEETNTSIAELK
                     IDIDSRFNVMKTDQDGINERLNKFQRETQRTLTDLQSAVTRMSRELSDLDKRVKTLET
                     PPTPAKK"
     gene            1160..2533
                     /locus_tag="PLI55_01965"
     CDS             1160..2533
                     /locus_tag="PLI55_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99636.1"
                     /translation="MKKYLFIILIAFTTTLALPACSGGKETKKVERRTEQTKVTEKTE
                     PKKVQSPKTSTETTNLETVRKDTAGMVMQSVTAVKESQLDSVTPAKKDVQNEPDSTPL
                     TGNTSELAIQPSPVITDEIKIDADLLPTSNETNGVLESKVVTEKPGLCTKEAVTSAKV
                     LKILIFDDIFFDADETETPNLTFNSNYIVTLNKVVKALKSDPDIKIRLKGYAMDTGTK
                     SGNAIIKSQKRAITVGKLILDLFPEEEKDSVANRIEIVPVGADEMLIESDNQVKELLN
                     HRVSIELFVGEVVNPTIADFVFHGKAPEPEVTSTAPKPVQREKAKTPKSRPRTRDMLY
                     ETGNDLFQQKKYEDAISVYTEFINLDPQHPLADNAQWWIGECYYFLGDYVSALNAYRK
                     VFGLGDGNKSAYAQLRMGYCYNRMNQVDLAVSAWEKVIRDYPNAIEEVTKAKKAIQIY
                     SGDVNQD"
     gene            complement(2627..2710)
                     /locus_tag="PLI55_01970"
     tRNA            complement(2627..2710)
                     /locus_tag="PLI55_01970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2674..2676),aa:Leu,seq:caa)
     gene            2779..4590
                     /locus_tag="PLI55_01975"
     CDS             2779..4590
                     /locus_tag="PLI55_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPA99637.1"
                     /translation="MSDFSYSTISEMFVGITDKYTDRPMYKYKKDGKWLGLTFSEVRN
                     RVEQISGGLKALGMKAGEHVAIIAANNPLWAMTDYGVICAKGVVVTIYPTLTAKQVWW
                     IARHSDSRFIFCGDREQTEKVIPLLNELPEVYKVIIMDDTKIDHPKAIMMSELMQMGA
                     EYRGQNPSAFLEDIHNTKEDDLLTIIYTSGTTGEPKGAMLTHGNLTSNITGALQIVEA
                     NENDTFLSFLPLSHGFERMAGHFLATGSGACISYAESIDTVAENMLEVHPTLMVAVPR
                     FYEKVYARVIDSISTSSKISQKIFWWAINVGKKAVFLKSENRNTGAILDNQLKIAQKL
                     VFSKLYEKIGGNLRFFVSGGAPLSKEIAQFFQAAGIPILEGYGLTETSPVISVNPLSK
                     CKVGTVGPKLPNIEVKIASDGEILTRGPHVMLGYYKDEASTRDVLDADGWFHTGDIGM
                     IDGDGYITITDRKKNILVTSGGKNVAPQPVENALVTSKWIEQIVVIGDRRKFVSALVV
                     PAFVNLEDWAKENNLQFHSREELIALPAVKELYDRVIAESMEGFAQFEKIKKYVLLPS
                     EFTIESGELTPSLKVKRSVVEKHYADLIESMYQEAES"
     gene            4591..5583
                     /gene="purM"
                     /locus_tag="PLI55_01980"
     CDS             4591..5583
                     /gene="purM"
                     /locus_tag="PLI55_01980"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPA99638.1"
                     /translation="MSVSYKDAGVDIAAGEQAVKEIKNLVKQTFTPNVLTEIGLFGGC
                     FAFPVKNYRQPVLVASTDGVGTKLIVANLLDRHNTVGQCLVNHCVNDILTTGARPLFF
                     LDYIGTGQLKPAITTEIIRGISLACKANGCALIGGEMAEMPDIYQTKDYDLVGTIIGV
                     VEREQMLIGRVGKGDVLLGLPSTGLHTNGYSLARKVLLTKYSVTDYIPELNATIGDAL
                     LAIHRSYLNVIEPLLADPDLHGLSHITGGGIIGNTSRIVPENLTLKINWQAWEWPPLF
                     KFIQKTGEIDIEEMRRVFNLGIGMIIICAASAVDKFSQVLQKSGEKAVILGEVC"
     gene            5618..6628
                     /locus_tag="PLI55_01985"
     CDS             5618..6628
                     /locus_tag="PLI55_01985"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPA99639.1"
                     /translation="MSIAVLGAGSWGTTIAIHLYNKKLPVKLWEYFPENVAYMNKTRR
                     NPLLEGIPIPRAIPILNDLTEAVKNANFLVLAVPSQSMRSLLEKIKGQIEPDTIIVNL
                     TKGIEQNTLKRMSELITEILNHPPEKIVTLHGPSHAEEVARQVPTAVVAASINEETAQ
                     LVQEVFHSPYFRVYTNTDIIGVEIGGAVKNVIAIAAGICDGMRLGDNAKAALMTRGIM
                     EIVRLGLRVGGREVTFAGLSGIGDLIVTCNSQHSRNRYVGEEIGKGRKLDDILNGMSM
                     VAEGVTTCLTVHQMAEKYKVVMPISEEIYKVLFKNKDPQTALWDLMNRDPKQERHSLS
                     KY"
     gene            6625..9354
                     /locus_tag="PLI55_01990"
     CDS             6625..9354
                     /locus_tag="PLI55_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPA99640.1"
                     /translation="MISSLKRLLLLSGCFVTVTMAQELHTPAPQPISGTPFLWNKYPE
                     IVFHHRLAKPVAGDTTFFIRKNVEDNSAEFIEIAFLRLINTAEIEIYAEKTEFDAGRI
                     TQSDAERVRDIMLYDTPPGSINPAKGIYYNEIDIFGALPDKDGNGKLFVLLIDVRDNY
                     EAGKSDTYVAGYFDPLDQITGKGNYGEIIYIDTNPADAKDLGTLSIVAHELQHLIHNF
                     YDSDEDVWVTEGLSELAPRLLGLPARSFASFLNETNRSLMDFDGSIADYAKVGLWTYY
                     IYQRFGLDVIKKIFNNKNDSFNGFIGGLSSAGYDGSVAELLRDWFVANLLNDPTMGDG
                     QYGYGGAAISPISSNYFHSNFTEGEQISLALNPAAAVYIQFYSGQNIEFVMNYQSSYS
                     FGLAVIKHYETPEITFVTCNGTGTYNFSDETFGSDYAKLTIIPYWPAEIAATVQANIS
                     YSATGIGGYAESELGYDNDMLDYFIRLEDAEAAEKFDLNLSEATLSAIKILTYDSSPV
                     TIKIYSNLAEAPLLTMSGIIPDEQAWTKIILGNPIDLADLSSFAVSVTSSGNSLGYSS
                     AQDGFGHAYIKTDEIFHDLRNYIVTGGLEDVSLTGNWAIRAIVSGKIMIPPRIILMPD
                     SLWFWHDEYTQTFRISNSGTEALEWEISGELPNWVKIEPLFGTVNIGSAEIAVTIDRN
                     LLTSGFHDINIPIISNGGDESLYVSVLKRNTSTAQSALIPTEMVFSDDISRTTLPIFN
                     IGIGSSQFAFWSEEACLAFLPSSGFIPENDTINVEVFLDRSAASDSILAFYFFDGVDT
                     LAYECIYSDWQGETATHFTILNPFPNPFLPGENPLIYFPFRLTVEKPVTALIFNINGQ
                     EVYSEKINNPKIGLNVWTWDGHNQKGNPVASGVYFIVFRQGERRARQKLVLLR"
     gene            9666..10715
                     /locus_tag="PLI55_01995"
     CDS             9666..10715
                     /locus_tag="PLI55_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPA99641.1"
                     /translation="MLDITTKENIILYLSQWIEIVRIEDRDKYLVIEGRQRDTTSLRK
                     IARNSRNLISSIKKISTGSIDRFIVKPGKIEKRIPTVNLVLFLITILTTLLAGAMMEG
                     ANPFAHPLDLLKGVPFSVTLMVILGFHETGHFLMARKHNVDATLPYFIPAPTIIGTFG
                     AFIKMRSPVTRRQALLEIGAAGPICGFLVATPALFIGLKLSEVVSLSEAIGIQLGDSL
                     LMKIASALIFPNLEVGQDILLHPVGFAAWIGMLVTMLNLLPIGQLDGGHIAYALLGDK
                     YPYVAWGALIATLALSFVSLNWLVWGLLVFFLVRVKHPPIYDDNSPVTLREKLIGLAS
                     LVIFILTFIPVPFKQ"
     gene            10729..11790
                     /gene="mltG"
                     /locus_tag="PLI55_02000"
     CDS             10729..11790
                     /gene="mltG"
                     /locus_tag="PLI55_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPA99642.1"
                     /translation="MKRIKYNFGQSVKRPLVYIPLIIFFIVILYLAILVTPLSSSNTN
                     QVITIKPGTNVQEVSELLYEKGIIKYPKRFILATKMMGKERKLCAGRFDLYGAINYYE
                     LIKTLSDLESVTVRVTIPEGLRAREIARLLENQIGISADDFMQKVMDSRFTHKLGVEA
                     NNLEGYLFPDSYYFIAGDLPENVISRMVERFWEVVNDSVQAEIRRTGYTLHQIITLAS
                     IVEGECQVDFERPLVASLYYNRLRRRIRLEACPTIQYIIPDSPRRILRQDLKIDSPYN
                     TYLHYGLPPGPVCNPGFKSIKAAIYPAKTKYLYMVSNGDSTYTHTFTANYEEFLAAKR
                     KLQKIRTQDKLVASEDYIN"
     gene            11787..>13872
                     /locus_tag="PLI55_02005"
     CDS             11787..>13872
                     /locus_tag="PLI55_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPA99643.1"
                     /translation="MKSLEDLNPEQCRAVEYNAGPTLIFAGAGSGKTKVLAHKVAYLI
                     ENKIVAPQNILAVTFTNKAARELGERVEKYIGKKARQVNIGTFHSICARLLRREIECL
                     GYSSNFTIYDAEDQLSLLKKIISDRSIILESTSPKNVLSRISYLKNHMIEPENFSARP
                     GFMADRFVAVLYPLYQGALRKAMAVDFDDLLILPLKIFQLKPEVLEKYRALYQYILVD
                     EYQDTNRPQFLLIEALGKKHQHVCVVGDDDQSIYSWRGADIRNILSFNEIFPQCRIFK
                     LEQNYRSTNNILKAASAVVSHNKQRATKTLWSQKSDGDLLVKLDAETEYDEAALIASW
                     IQREILQNKRNFKDFAILYRTNAQTRVLEEILRRNNIVYVIVGGVRFYERKEIKDVLA
                     FFNVISNPHDDISLKRIINLPPRGISKTTVQHLEELALAKDISLFDAMSYADELNLAT
                     RAIESIKKFYDQINDLRSLRNTLSFEEWARVVIVSIGLRNYYKESEGEEATERLANLD
                     ELLNDISEFCQTNEKPSLESYLENVTLATSIDEWEDTKNAVTLMTLHGAKGLEFPVVF
                     ICGLNQGLLPLERDLTHEALEEERRLFYVGLTRAKEKVFLSSSRQRMRAGEVAYSSES
                     MFLNEIPPELITICDFREGIISSKRQPRTSNRSTARKIKDTFERVESNPIKIAGINSS
                     SSEKTSPTSRTIE"
     assembly_gap    13873..13972
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13973..14366)
                     /gene="rnhA"
                     /locus_tag="PLI55_02010"
     CDS             complement(<13973..14366)
                     /gene="rnhA"
                     /locus_tag="PLI55_02010"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020053466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HPA99644.1"
                     /translation="MKIIYSDGACSGNPGHGGWATLIFNENQLLKVLAGYQENTTNNR
                     MEIIAALNGLKQLQPGEKAIIYTDSQYLRNGITTWLPNWKKRNWKTADKKSVKNQELW
                     LELDRLNHPGITWQYVRGHSDNLYNEICD"
     gene            complement(14367..>16152)
                     /locus_tag="PLI55_02015"
     CDS             complement(14367..>16152)
                     /locus_tag="PLI55_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99645.1"
                     /translation="RYCYFLSVARFVKCILIIGVLTVSLSAQWYFKIVDAIHGPIEYR
                     TPFKLTPFEFKTGIQTFGFDGQPLLFDSPGISFNGYKTSWERTMNSLELDLLKFNWLV
                     YILPQNLLDFQTGIGAKYTYSLSEQKLPETWPQRLPGSVDQLFLSPRIYEFNLNQTVI
                     YQWAPNFYNYLTISYGQASGSAYKTHSNDYFLHQKGHTYSFALGFKFLGSINYKLKEG
                     YGVEIRYTVGDFGDLEDPHNISPIKKINFNTVGISLTFNSNLGGGRSTGDEAQALYQA
                     NDFIAAKATFEQFIADNPHHPSRFKARRMIKECDKRIPYQEVTLAELFIARQNYSIAT
                     EYLARAQVTKDKTLLARINSNYERMIKQFVDTMNVMISNNRIDEAEQFLNTIAKLNLP
                     NTNELIYCYYSEIYFHRGAVFTEYGYWEKAIDYFDRAIQYYPPIRQRIEPYLVQIADG
                     YIKDANLSVDKKSIALALESLKQATALRPDIHYLTAPHIKNLEAGIEYLKQQAAKQKA
                     QESISKTFNPPPDAPKPDIGMSADQIKSILGQPGSQTRLETSGGRIYELWIYLFPDGK
                     ELQIYFDNAKVVKMEALSPIDVISEMDE"
     assembly_gap    16153..16252
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16261..16908
                     /locus_tag="PLI55_02020"
     CDS             16261..16908
                     /locus_tag="PLI55_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HPA99646.1"
                     /translation="MVILLAVLNLAGQSKNLYGKFTIARLKYDGGGDWYSNPSSIPNL
                     LKFLRENLNMDVASDEVRVGIMDKDFFAYPYLYMNGHGNVRFSDEEVLRLRQHLQNGG
                     FLHADDNYGMDQSFRREIKRVFPDNDLVELPFDHPIYNILYKFPHGLPKIHEHDGKPP
                     QGLGIFYKGRLVVFYTYECDLGDGWEDPEVHNDPEEKHQAALQMGANIVLYSLMQ"
     gene            16909..20301
                     /locus_tag="PLI55_02025"
     CDS             16909..20301
                     /locus_tag="PLI55_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99647.1"
                     /translation="MGNNQSSYNRIERKLNDLRRQFARHVLQVGLLKVLLLIIIVTFL
                     IVVVEGFRYFKGSVRSDILFFTVGFSLIFLAIPIVYYFQIIRNRISAYDDYHLARRIG
                     ADQPEIKDDLLNALQLRELLNKETRGYSSELIEQALERIARKIEPLNFDRIIPSQEVR
                     KSLRNLAIAIFCLVICIASSPAYFKNSTERLLHPRIEYPVKLPFSIESTRASSGVLGG
                     DSVTISFQCQGRFPHYLTLDLQYPDYTRQEILTVDDSGRSQYLIENLRHDLVYSAYYQ
                     NHSPFKPWRRIGTPPDTIRVINRPEILYVKSRFIYPEYTGLPAQTQETKSAEFNLLAG
                     TKISLEIRINKEIKEGHLSFASGNVLNLATNGDRARGEFIVKNDDQFEIIVADEKQVT
                     NINPIKYRLSVIPDAYPTITMLSPKNDLDLNADMVIPLGIRISDDFGFTRAAIRYRVI
                     KKYADYQEEETEITFPLAKLNITWQEVFYPWQISELRLGPEDAVEFRVDIYDNDNISG
                     PKKASTDWLIARFPSLNELFSSVNEQQDEIYSSSEEVLKSLETTKKVLDEISRELLKK
                     PELKWEQKKQLEEEMKKTREAGEKITKLSEQLDDLIEKSRENQLFDAETLEKLAQVQE
                     AFQDIMTPEMREALDKLQAAIEKMDPKEVQKALNNFKTSQEQFSQELDRLLKIFRRIQ
                     IEQNIDELVRRFADLSERQRQVANELRKTAPNDQQKLNELAQAENAIKNDTEIAQDIM
                     KRTAADMAEFPMMPNKELQQLIDEMQAMDLPQEQSQASSAMRKGDKSQAEESAQNSQQ
                     NLQKLLERMEEFRTEFQRRNMADVMTDFRRILNKTMQLSYRQEELTEEIKNTPRQSDQ
                     IMDIAVKQSETRQNLSRLISDLIDLSGKTFGVSPRIGKNLGQSANSMNAAIAYMEERD
                     PGLAALSAENATASLNMTALELMAAMQELQKSGSASGFENYLQQLQELAGMQQGLNEE
                     TQMLGLGSPGDQGTLQQLAARQQQIRQSLEKLRDEMQGQGSTKQSGDIGGIAKDMDEV
                     IKDLENNQVLRRTLERQQRILSRLLDAQKSLRTQDFKEERQSKTGQEIVRESPDGLPS
                     NLGERRTLLQENLEKALRDGYSKEYENLIRRYFEELSKESVE"
     gene            20298..22238
                     /locus_tag="PLI55_02030"
     CDS             20298..22238
                     /locus_tag="PLI55_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99648.1"
                     /translation="MKLEWRLGLLILLIFWLTAFVAAQPVKVVSPEEKLMNLRFNQAQ
                     RLEQEGQLTQAAEIYRALVDAQPANYLYYSNYVNLLFRQKNLPELERVITRFMQLNPL
                     HENAAIDLGKLFYVRGDSAEAFREWQKAVVKFNYSVSFYRALFNEMVGLRLYDEAERL
                     IAAARAHYRQPDLLTMELANLQIARGKYSSAARELVLYARANPRYYNMIAGQILRFPT
                     DSSLFIQIDSLLKAELLLLPDQAALHRLRADFLFKNGHYNEALEEIFQVEALTGNHGD
                     QALAMAKNLVQIRRYELAQDFYTKILAKNELQGIAPQALLGLAESFEKAGLEQTTAEP
                     LHYFYPGNFFFNTDFVQQINQEQSSLQKAFAIYDSLVANQPNSIYSAQALYRLADLRF
                     RAVRDFDGALNLLRQAQNITRDRQLILNCALRIGEVQIARGDPDAALKYYQAEIVRWE
                     GTPGEKDLRVHLALAQFLAQEFDSLDIELKNLIPLLGPKHALFNDVVGFDGFFRANYS
                     ETDAAGKKAFSEFATAELLLRQNKLSEAEKIYAFILQNYPQTPIISAARFRLTQILLQ
                     FNRYAEAEATAESFFASENENADQIAFMLAEVADYREANYQAAAYYYELILEKYPNSF
                     LIDNVRKRLRELQRLSGVKKEI"
     gene            22244..23515
                     /locus_tag="PLI55_02035"
     CDS             22244..23515
                     /locus_tag="PLI55_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014796718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HPA99649.1"
                     /translation="MKRNRPLIVLIGLILILANLSFAQKILIPMDQTQSDHLKAYGIV
                     YWSLKQGVNVEWLLNYRGGSFLLDNYPGIVKELRLRGVAYEEVSAAQNLQIYATIEEN
                     NMDVVLLEKAAKIAVYSPPNKQPWDDAVTLVLTYAEIDYDVLWDEEVLTGKLPEYDWL
                     HLHHEDFTGQYGKFYASYHNQAWYQNEVAENEAIAAKLGFRKVSEEKKAVARTIKKYV
                     NEGGFLFAMCSATDALDIALAAENVDIVAEIYDGDPADPRANEKLDYSKCLAFENFKL
                     YLDPMLYEYSDIDYPPSFQAVAPGAEADYFTLFEFSAKWDPVPTMLTQNHVGVIKGFM
                     GQTTGFRRDRIKKSVVVMGEVVNGEQVRYIHGNHGKGTFTFLAGHDPEDYQHFVGDPP
                     TRLELHKNSPGYRLILNNILFPSARKKERKT"
     gene            23530..>23837
                     /locus_tag="PLI55_02040"
     CDS             23530..>23837
                     /locus_tag="PLI55_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPA99650.1"
                     /translation="MKEIILKTNRRIELIDITAQVAEAVRSSGIREGICVVYCPHTTA
                     GLTINEHADPNVASDIVNRLSKLVPHNENYAHIEGNADAHIKSTLTGNSVQLIVNNGN
                     "
     assembly_gap    23838..23937
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            24558..25100
                     /locus_tag="PLI55_02045"
     CDS             24558..25100
                     /locus_tag="PLI55_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPA99651.1"
                     /translation="MKLKNERSVLVIIDVQTKLLSVMYNYPLLLENLKKLIRGSQVLD
                     IPIIYTEQYPTGLGPTHPEIASLLSNTPPIIKMAFSCCSEENFMNEIHKINRDQIIVC
                     GIESHICVYQTCRDLIDKKYEVHLVTDCIASRKLENTQLTVSKLLAYGVHPTSVEMAL
                     FEMLKDASNDAFKKIAKIIK"
     gene            25113..26291
                     /locus_tag="PLI55_02050"
     CDS             25113..26291
                     /locus_tag="PLI55_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPA99652.1"
                     /translation="MNIEQIQTEIRKQKVDGWLFYDFHNRDKMAYHILGLNPNMFVTR
                     RWYYFIPAIGAPIKLVSAVEATKIDNLPGEKIIYRSWEQQHSSLRTMLKNSRRIAMQY
                     SPNNNIPYISVVDGGTIELIRSFGVEIVSSANLVQIFEAVIDEEGYRSHLRAGQKVQQ
                     IKNEAFALISSRIKEQKSVTEFEVQQFIVKRFGEEDLTDDNEHPIVGVNEHPADPHFE
                     PKAENAYTIKPGDTVLIDLWAREQSERAIYYDITWCGFVGQNPPAKYVEIFNIVVRAR
                     NAARDFIRRKMADNQPCYGYQVDDACRNVVKEAGYGDYFVHRTGHSIGHYVHGNGVNI
                     DNLETKDERELVPGICFSIEPGIYLPGEMAVRSEINVFITKDREVVVAGDEQEKLLLL
                     "
     gene            26330..26917
                     /locus_tag="PLI55_02055"
     CDS             26330..26917
                     /locus_tag="PLI55_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99653.1"
                     /translation="MKKTLVVFCVVVILILASLATAGTPTTQKGSAAMWFNFNGLSDM
                     SYQNSAIGLNYLFKDKTGAGIALNFSHDSEDDGIEEKEKTTALGMDLYLIHYPFQKGP
                     VALYLAPGVGFNFSSKKDKDFGDIHINSTYMWLGGSVGVEWWIFDQVSLSASNWVGLE
                     IDLLNDKENDDKMTDVHLGILGSSTASIVLAFYFK"
     gene            complement(26975..27970)
                     /gene="dusB"
                     /locus_tag="PLI55_02060"
     CDS             complement(26975..27970)
                     /gene="dusB"
                     /locus_tag="PLI55_02060"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPA99654.1"
                     /translation="MDSVQKITALTIGKVCIKPPLILAPMAGYTDQAFRQICKANGAG
                     LVCTEFVSSDGLVRSSEKTYDYLRFSDFERPISMQIFGNNPVVMAEAANLIENEFHPD
                     LIDINAGCSVRKVIKKNAGSALLKNLPLLEKIARAVVEAVSVPVTVKMRSGWDRQSII
                     AVEIAQRLEQTGIKAITIHPRTAVDGFRHPADWSLIKEIKKKISLPIIGNGDIFTPAA
                     ALRMFAETNCNAVMIGRGALENPWIFRQISDLLEDKPLISYDSPTERVKLCRRHLEME
                     IQYRGEVVAHKEMKKFYRWYLRGFGHVSEIREKLVRSKSVQEAFNILDELAHVTK"
     gene            complement(27982..28887)
                     /locus_tag="PLI55_02065"
     CDS             complement(27982..28887)
                     /locus_tag="PLI55_02065"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPA99655.1"
                     /translation="MKKLVITGASGFLGGHIANLAVSRFQTLGLYSTYPFNLKGITTQ
                     RIELSSPSSIIVVLKRFKPAVVIHNAALANPDQCENQPDFARQNNVIATEHIASWCHK
                     NNARLIYVSTDMVFDGQKGNYSETDRPNPLSVYGKSKYEAEKLAMANNPDTVVARLAL
                     MYGRGIFPRAYNSEWLLRELLRRKKEPKLPPLKLYNDQYRSMLMVTNAAQALIELALS
                     HFCGIIHIGGPERISRFAFGEKLCRKLNLPLTFIQAVPYDEHQLTAPRPRDLSLDISL
                     ARSFLKTNFLAVDDGLDEMLMSNSD"
     gene            complement(28973..29668)
                     /locus_tag="PLI55_02070"
     CDS             complement(28973..29668)
                     /locus_tag="PLI55_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99656.1"
                     /translation="MPSLEKSKKSQKKNQKIDKLANLTISVFFLNILIISSVLILVFF
                     IFGQTPDDKPSGWRTLIAEHLNRYPAMQPADVYKLVYQGTFGPAHLGNDSIQISTGLE
                     NELAIITPDSSAPLSESITPSGKYVRLNLKRCKAEQIQTAKIVIAILNSIPQSSADSS
                     RFIRRWETIEKLVVEGSLPIPRSDFFTFSDSIKKTGYPVIHHSSQYIKNYQPAYRVID
                     RRYLNLITDSLSQ"
     gene            complement(29616..29813)
                     /locus_tag="PLI55_02075"
     CDS             complement(29616..29813)
                     /locus_tag="PLI55_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99657.1"
                     /translation="MLRLLLLFLIFYLLWKMVKPRNLRQPKPNNPKPKKPRTTLKIDK
                     SQIEDAKFREIKEEPEKEPKD"
     gene            complement(29813..30457)
                     /locus_tag="PLI55_02080"
     CDS             complement(29813..30457)
                     /locus_tag="PLI55_02080"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPA99658.1"
                     /translation="MKNLYFLAIEGVIGAGKTSLARLMAEKLNARLVLEKFEENPFLE
                     DFYRDPERYAFQAQLYFLLSRYRQQIELKQIDLFTNLLISDYMFAKDKLFAILNLDEK
                     ELSLYNLVAQHLEKDLPKPDLVVYLQASTDRLISNIKKRGRSYEKSINRDYIDSLNQI
                     YNEFFFRYKDSPLIIVNTNEIDFVNNENDLNELIREIKQPISGTIYYNPAKKIL"
     gene            complement(30454..30993)
                     /gene="folK"
                     /locus_tag="PLI55_02085"
     CDS             complement(30454..30993)
                     /gene="folK"
                     /locus_tag="PLI55_02085"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPA99659.1"
                     /translation="MKSSGVFVALGSNLNLREKNLSAALELLSHNPAISICRMSAVYE
                     TAPKYNPGQPKFLNMVVEIATTLTPLQLLEFCKDIEVRLGRHFPHFKNSPREIDLDII
                     FFNRLIVESPTLTIPHPGLYERKFVLVPLAEIAPEFICPKTAKSVAELLRICPDIEQV
                     VRIGQLDELLTIPVTTGVL"
     gene            complement(30990..31367)
                     /gene="folB"
                     /locus_tag="PLI55_02090"
     CDS             complement(30990..31367)
                     /gene="folB"
                     /locus_tag="PLI55_02090"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPA99660.1"
                     /translation="MGIVRLNNMVFYGYHGVAEPEKILGGKIEVDVELEFDMTKVITT
                     DHLADTIDYEKVYQLVQGVVTQSKFFLIEALAGKILSEIFNHHLVESVRVRIRKPNAP
                     IKGVLDTVEVEIFRRRDQMGEKS"
     gene            31701..32132
                     /locus_tag="PLI55_02095"
     CDS             31701..32132
                     /locus_tag="PLI55_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013554948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPA99661.1"
                     /translation="MAITKATPKSVTRKSPYTIDRFFDPFFDWDTETSLVTFAPEVDI
                     EEKDTEYVVTVELPGVKKDDVKISLKDNVLTISGEKKRVKKINKKNFYQCERVFGSFE
                     RSFRLPELVDHEKIEAEFKDGILTIVVPKSPESKPKSIEIK"
     gene            32169..32612
                     /locus_tag="PLI55_02100"
     CDS             32169..32612
                     /locus_tag="PLI55_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPA99662.1"
                     /translation="MAITRWTPRSSWLDVRNEFDRLFESIFNPQYEEETSLLAFSPAV
                     DIIEEDKAYNIQVELPGMNKDDIKIAVKDNYLTISGEKKRESKVEDKNYQRTERIYGS
                     FQRNFRLDDSVEADKIEAEFKDGVLNIVIPKKKESLAKQIEVKVK"
     gene            32832..33200
                     /locus_tag="PLI55_02105"
     CDS             32832..33200
                     /locus_tag="PLI55_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPA99663.1"
                     /translation="MKVFIADDSEILRKHLTNLLTEIPGVEIVGYGEDTESAISGIDT
                     QKPDVVILDIRMPGAGGIYVLKTVRQNRPDLRIIVFTDYPYPQYRKKCLEEGTDYFFD
                     KSSESEKLIELVRKMAGGKA"
BASE COUNT         9719 a         6498 c         7405 g         9499 t
ORIGIN      
        1 gaattccgtc aagtcttaca acagttgact tctaatcaac cttcagtcga gattaaatta
       61 aatagtgtta ttcgaggggt ttgtcgtcaa ataatccgag aaattgatcg ctgggaagac
      121 ctaaagcaag aaaggtggta gtttgaacat agaaaaatat tgataattaa aatggtattt
      181 gtaaattaaa tattgatatt ttaaaaagaa tacaacatta attatggaag ataaaaaagt
      241 cgattttagc aatgaggaaa gccgcaaaaa ttttcttata actaaatttg acgacttatt
      301 tcaaggtatc aataacctct atggcaaaga gttaatggaa gagttatttc atagaatgga
      361 aaaaaccatt gctatttttc atcaagacgt cgagaatcta attaaagaac taaaatcgtc
      421 aaattctacg ggcagtactg atcaaccgat tatgggaata tctgatatac ctcaaacgcc
      481 agctgaacct aacccttttt cggcaaatga agataatgac tgggctaaac acctcggtta
      541 aaatattaac aataagtaaa ggtacaggag acatgaatga aagaactaat taaaaccata
      601 gcattggtgc ttgtagttgt agcggtcgtt tattttttct ttgaatttga aaaagtaaac
      661 aaacgcctgg acattttaaa tgcccgtgat tccacccata gtgtaaccat caacgatttt
      721 gatcataatt tacatgatct gagtctggaa tttgtcggga gaggcaaaca tattcaacag
      781 tttcagcaat cgctgaaaga tatgaattca cgattagaca gcgtggactt ggcgctaagt
      841 gacaaaatcg aagaaactaa caccagcata gctgaattaa aaattgatat cgattctcgt
      901 tttaatgtta tgaaaaccga ccaggatgga attaacgaaa gactcaataa atttcaacgt
      961 gaaactcagc ggacccttac tgatctacaa tccgcggtta cccggatgag tcgtgaattg
     1021 agtgatctcg acaaacgtgt aaagaccttg gaaactccac ctaccccagc aaaaaaataa
     1081 tctgatagat aaataatata tttttatcat ctggggataa ttggatttgt taagcattaa
     1141 aatcttagag gagggagagg tgaaaaaata tttatttatt attttaatag cttttaccac
     1201 cacattagca ttgcctgctt gttctggtgg aaaagaaact aagaaagtag aaagaaggac
     1261 tgaacaaacc aaagtaactg aaaaaacaga accaaaaaag gtacaaagtc caaaaacttc
     1321 aaccgagact actaaccttg aaacggttag aaaagataca gctggaatgg ttatgcaatc
     1381 agtaaccgcc gtgaaagagt ctcaattgga ttctgttact ccagccaaaa aggatgttca
     1441 aaatgagccg gattcaactc ctctgactgg taatacttcc gagctagcta tacaaccctc
     1501 tcccgttata acggatgaaa tcaaaataga tgctgatttg ctaccgactt ccaatgagac
     1561 aaatggagta ctcgaaagta aggttgttac ggaaaaaccg ggtttatgta caaaagaagc
     1621 cgtaacttcg gctaaggttt taaaaatcct tatttttgat gatatatttt ttgatgccga
     1681 tgaaacagaa actcctaacc ttacatttaa ctccaattat atagttactc ttaacaaagt
     1741 agtgaaagct ctgaagagtg atccggatat caaaattcgg ttgaaaggct atgcaatgga
     1801 tactggtacg aaatccggta atgctatcat aaaatctcag aaaagggcga ttacagtggg
     1861 gaaattaata cttgatttat ttccagaaga agagaaggat agcgttgcca accggattga
     1921 aatcgtgcct gttggtgctg atgaaatgct gatcgagagt gataaccaag taaaagaact
     1981 gctgaatcac cgggtgtcaa ttgaattatt cgttggtgaa gtggtcaacc caactatagc
     2041 ggattttgtg tttcatggaa aagcaccaga accagaggta accagtacag cgccaaaacc
     2101 tgttcaaaga gaaaaggcaa aaacaccaaa aagccgcccg agaactcggg atatgcttta
     2161 tgaaactggt aacgatctat ttcaacagaa aaaatacgaa gatgccatca gcgtttatac
     2221 tgaatttatc aatctagatc cgcaacatcc gctggctgat aatgcgcagt ggtggatcgg
     2281 tgaatgctat tattttcttg gcgattatgt cagcgcctta aatgcatatc gcaaagtatt
     2341 tgggctgggt gatgggaata aatctgctta tgctcaactg cgtatgggct attgctataa
     2401 ccgaatgaat caggtagatc tggctgtaag tgcctgggaa aaagttatac gagattatcc
     2461 gaatgcaatt gaggaagtaa ccaaagccaa aaaagcaatt cagatttatt ccggcgacgt
     2521 taaccaagat taaataaata tttttttatc agctattaaa gtgaaatcaa tgaatccaaa
     2581 tattacattg atgcggagtt gctttgatat atgattgtgg tttttgtgcc gagaggggga
     2641 atcgaacccc catggaccga agtccggtgg attttgagtc cactgcgtct acctatttca
     2701 ccacctcggc aaaaagcgcc ctaaaattaa ctctttacaa gttaaaaaca ataaaaaagt
     2761 aaatataggg agttgattat gtcggatttt agctatagta ctattagcga aatgttcgtg
     2821 ggcataacgg ataaatacac tgatcggcca atgtacaaat ataaaaagga tgggaaatgg
     2881 ctgggactta ctttttcgga agtgcgcaac cgtgtcgagc aaatttccgg cggactgaaa
     2941 gccttgggta tgaaagctgg cgaacatgtc gccattattg cggctaacaa tccattatgg
     3001 gcgatgactg attatggtgt gatctgtgcc aaaggggtag tcgtgacgat ttacccaact
     3061 cttacggcta aacaagtgtg gtggattgcc cggcattctg attctcgttt cattttttgt
     3121 ggtgaccgcg aacagaccga aaaagtcatt ccacttctta acgaacttcc cgaagtttat
     3181 aaagtcatta taatggatga tacgaaaatt gatcacccga aagctattat gatgagtgaa
     3241 ttgatgcaaa tgggtgccga atatcgaggc cagaatccga gtgctttcct tgaagatatt
     3301 cataatacca aggaagacga tttgctaact ataatttata cttctggtac gactggcgaa
     3361 cccaaaggtg ctatgctgac gcatggcaat ctcacttcga atattaccgg tgcattacaa
     3421 atcgttgagg ctaatgaaaa tgacactttt ttgtcgtttt taccactcag tcatggtttt
     3481 gaacgtatgg cgggacattt tttagccacc ggttccgggg catgtatcag ttatgctgag
     3541 agtattgaca cggttgccga gaatatgctg gaagtccatc ccacattgat ggtcgccgtt
     3601 ccgcgttttt acgagaaggt ttacgctaga gttattgaca gtatttcaac atcttccaaa
     3661 attagtcaga aaatcttctg gtgggcaata aatgtcggta aaaaagccgt ttttctaaag
     3721 agtgagaatc ggaacactgg cgcaattctc gataaccagc taaaaatcgc tcaaaaactg
     3781 gttttttcaa aattatatga gaaaatcggc ggcaatttgc ggttttttgt ttccggcgga
     3841 gcacctctct ctaaggaaat tgcccaattt ttccaagccg ctggaattcc gattctcgaa
     3901 ggctacggtt taacggaaac atcaccagtt atttccgtta atccgctgtc aaaatgtaag
     3961 gtcgggacag ttggtccgaa actccctaat atcgaggtga aaatcgcttc ggatggcgaa
     4021 atcctgacgc gcggcccaca cgttatgctg ggctattata aagatgaagc ctctacccgt
     4081 gatgtcctcg atgcggacgg ttggtttcat accggcgata ttggaatgat tgacggggat
     4141 ggctatatca ccattaccga tcgtaagaaa aacatattag tgacttccgg aggtaagaat
     4201 gttgctcccc aaccggtgga aaatgcgctc gttacctcaa agtggatcga acaaattgta
     4261 gtcatcggcg accgacggaa attcgtatcg gctttagtgg tgccggcttt tgttaatttg
     4321 gaagactggg ctaaagagaa caatctgcag tttcacagtc gggaggagtt aattgccctt
     4381 ccagctgtca aggaattgta tgaccgagtg atagccgaat caatggaagg cttcgcccaa
     4441 ttcgagaaaa ttaaaaaata tgttctatta ccatcagaat ttacaatcga gagcggggaa
     4501 ctgacaccgt ccctaaaagt aaaacgttcg gtagttgaaa aacattatgc ggatttaatt
     4561 gaatcaatgt atcaggaggc agaaagttaa gtgagtgtat catataaaga tgcgggcgtt
     4621 gacatcgccg ccggtgaaca agctgtcaaa gaaattaaga atctggtaaa acagactttt
     4681 acccccaatg tattgactga aattggctta ttcggcggct gttttgcttt tccggtaaaa
     4741 aattaccgtc aaccggtttt ggtagccagc accgacggag ttggaacaaa attaatagtt
     4801 gccaatctgc tcgatcggca taatacagtc gggcaatgtc tggtaaatca ttgtgttaat
     4861 gatattttga cgactggggc gcgccctttg ttttttctgg attatattgg aactggtcag
     4921 ctaaaaccag caataactac tgaaattatt aggggaatat ctctggcatg taaagccaat
     4981 ggttgcgcct taattggcgg tgaaatggcg gagatgcctg atatttatca aaccaaagat
     5041 tatgatttgg tgggaacgat catcggagtg gtggaaaggg agcaaatgtt aattggtcga
     5101 gttgggaaag gtgatgttct gctcggttta ccttccactg gtttgcacac caacggttat
     5161 tctttagctc gcaaggtgct cttgacaaaa tattctgtca ctgattacat tccggaatta
     5221 aatgcgacta tcggcgacgc cttgctggca attcaccgct cctatttaaa tgtgattgag
     5281 ccattactag ctgatccaga tttacatggg ttgagtcata ttactggtgg cggaatcatt
     5341 ggtaatactt cccgcattgt ccccgaaaat ttgactttga aaatcaactg gcaagcctgg
     5401 gaatggccac cgctttttaa attcattcaa aaaactggtg aaattgatat agaagaaatg
     5461 cgccgcgttt tcaaccttgg aatcgggatg attataattt gcgccgcttc agccgttgat
     5521 aaattttctc aggtattaca aaagtccgga gaaaaagcgg ttattctcgg tgaggtttgc
     5581 tgaatatatt ttcatgctaa gaaaaattcc taaatcgatc tcgatagccg tattgggcgc
     5641 cggtagttgg gggacaacga ttgccatcca tctttataat aaaaaattgc cggtcaaact
     5701 ctgggaatat tttccggaaa atgtagcgta tatgaacaag actcgtcgaa atccgctttt
     5761 ggagggcatt ccgataccgc gcgcgattcc gattttaaat gatctgactg aagccgtgaa
     5821 aaatgctaat ttcctggtgc tggcggtgcc gtcgcagtcg atgcgttccc tgctggagaa
     5881 aataaaaggg cagattgaac ctgatacaat tatcgttaat cttaccaaag gcatcgaaca
     5941 aaatacactc aagcgtatga gcgagttaat tactgaaatt ttaaatcacc caccggaaaa
     6001 gatagttact ctgcatggtc ctagccatgc cgaagaagtt gcccggcaag ttcccacggc
     6061 ggttgtagcc gcctcgataa atgaagagac tgcccagcta gttcaggaag tatttcattc
     6121 accatatttc cgcgtttata ccaataccga tatcatcgga gttgaaatcg gcggggcggt
     6181 caaaaacgtc attgcgattg cggctggaat ttgcgacgga atgagattag gtgacaacgc
     6241 caaagccgct ctgatgacgc gcggaattat ggaaattgtc cgtctcggat tgcgagtcgg
     6301 gggtcgagaa gtaacttttg ccggattaag tggtatcggc gatttgattg ttacttgtaa
     6361 cagtcagcat agccgtaacc gctacgtcgg tgaagaaatt ggcaaaggcc gcaaactgga
     6421 cgatattctg aatggtatgt cgatggtggc tgaaggagtc acgacctgtt taacggttca
     6481 ccagatggct gagaagtaca aagtcgtaat gccgatttcg gaggaaattt ataaagttct
     6541 ttttaaaaat aaagacccgc aaacggcttt gtgggattta atgaaccgcg atcctaaaca
     6601 ggagcggcac tcactctcta aatattgatt tcttcactaa aaagattact acttttatcc
     6661 ggctgttttg tgactgtcac aatggcgcag gaactccata ctcctgcacc ccaaccgatt
     6721 tcaggtacgc catttttatg gaataaatat ccggaaattg tttttcatca tagattagcc
     6781 aaaccagttg ccggagatac tactttcttt attcggaaaa atgttgagga taattcggct
     6841 gagtttattg aaattgcatt ccttcgtctg attaataccg cagaaattga aatatatgct
     6901 gagaaaacgg aattcgatgc cggacgaatt actcaaagcg acgccgaacg ggtgcgcgat
     6961 ataatgcttt acgatacgcc gcccggttcg attaatcctg ctaaaggtat ttattacaac
     7021 gagattgata tattcggtgc attaccggac aaagacggta acggtaaact ctttgtgtta
     7081 ctcattgacg tacgtgataa ttatgaggca ggaaaatccg atacctacgt tgcgggctat
     7141 ttcgacccgc tcgatcagat aaccggcaaa ggcaattacg gtgaaattat ttatattgac
     7201 actaatccgg cggatgccaa ggatctgggt acactctcaa tcgtagcaca cgaacttcag
     7261 catttgattc ataattttta tgattcagat gaggatgttt gggtaacaga agggctctcg
     7321 gaattggcgc cacgcttgct gggtttaccg gcacgtagct ttgcctcgtt tttaaacgag
     7381 accaatcgtt cgttaatgga ttttgatggt tcaatagccg attatgctaa agtcggttta
     7441 tggacttatt atatatatca gcgctttgga ttagatgtga ttaaaaagat atttaacaac
     7501 aaaaatgact catttaacgg ttttatcggt ggtttgtcat ctgccggtta cgatggctcg
     7561 gtcgcggagc tattacgcga ttggtttgtt gccaaccttc ttaacgatcc gacgatgggt
     7621 gatggacaat atggttatgg tggggcggcg atttcgccaa tatcatcgaa ctattttcat
     7681 tctaatttta ccgaaggcga gcagatttcg ttggcgctca atccggcggc ggcggtttat
     7741 attcagtttt attccggcca aaatatcgag tttgtgatga attaccaatc gtcttacagt
     7801 tttggattgg cagtgattaa acattatgag acaccggaaa taaccttcgt cacctgtaat
     7861 ggcaccggaa cttataattt ttccgatgag accttcggaa gtgattatgc taaactgact
     7921 attattccct attggccagc tgagattgcc gccactgttc aagccaatat atcttactca
     7981 gcaactggaa tcgggggcta tgccgaatcc gaattgggat acgataatga tatgctcgat
     8041 tattttattc gattagagga tgccgaagcc gctgagaagt tcgatttaaa tttatctgaa
     8101 gcgactctgt cagcgattaa aatattaacc tatgattcca gtccggttac aataaaaata
     8161 tacagcaatt tagcggaggc gccgctatta actatgagtg gcattattcc tgatgaacag
     8221 gcttggacaa agattattct cggcaatccg attgacttgg ccgatttgag ttcatttgcg
     8281 gtatcggtta ccagttcggg taactcgctc ggctatagtt ctgcgcaaga cggattcggt
     8341 catgcttata tcaaaactga tgaaatattt cacgacctcc gcaattatat tgtgaccggt
     8401 ggtttggaag atgtttctct taccggcaat tgggctatcc gtgccattgt ttcgggaaaa
     8461 atcatgatac ctccgcggat tattcttatg ccggatagtt tgtggttctg gcatgatgaa
     8521 tatactcaaa cgtttcggat tagcaatagc ggtaccgagg cgctggaatg ggaaatttcc
     8581 ggcgaactgc cgaattgggt gaaaattgaa ccacttttcg gtacagtgaa tattgggagt
     8641 gctgaaattg cggtaaccat tgaccgtaat ttattgacca gcggctttca cgacattaat
     8701 attccgatta tttcgaacgg cggcgatgaa tcgctctatg ttagtgtttt aaagcgaaat
     8761 acctcaacag cgcaatctgc tctcattccc acagaaatgg tattcagcga tgatattagc
     8821 cggacaacat taccaatttt caatattggc ataggttcca gccaatttgc tttctggtcg
     8881 gaagaagcct gtctggcttt tttgccatcg agtggattca ttccggaaaa tgatacaatc
     8941 aatgtggaag tatttttgga tcgatcagcc gcgagcgata gcattttagc cttttacttc
     9001 ttcgacggag tagatacttt agcatacgaa tgtatttatt ctgactggca aggagagaca
     9061 gcaacccact ttactatcct gaatcctttc ccgaatccat ttctgcccgg tgaaaatcca
     9121 ttgatttatt tcccttttcg attgactgtg gaaaaaccgg ttacggcatt gattttcaat
     9181 attaacggac aggaagttta ctccgaaaaa atcaataatc cgaaaatcgg cttgaatgtc
     9241 tggacttggg atggtcacaa tcaaaaaggc aatccggtcg ccagcggagt ttactttatt
     9301 gttttccgtc agggtgaacg acgggcacgc caaaaattgg tgttattgag ataatatgtg
     9361 gctactatgc atttcatgtt gagtaaacta aaatgaaatt cagtgcggtt tctggttcat
     9421 tttctttgat acctgaattc aaatgtgaac tttacccaag taggttattg taaccggttt
     9481 tagtctgccg gtgtctaaag ttcggtcgaa tttttcataa tacgtgaata tctttaatca
     9541 ttaagttcaa tcatttagaa gcagaaactg ataagttgcc agacaaaagt cgaagaatta
     9601 tttgattgat tttaaggctg tattttgtaa gttctctaaa aattgggtaa tttaaacgaa
     9661 atgttatgct ggatataact accaaagaaa acattatttt atacctgagt cagtggatcg
     9721 aaattgtccg aatagaagat cgagataagt atctggtgat cgaagggcgc cagcgggata
     9781 ccacttcttt gcgtaaaatc gcccgcaatt ctcgaaattt gattagttcg ataaaaaaaa
     9841 tatcgactgg ttccattgac cgatttatcg tcaagccggg gaaaattgaa aaacggattc
     9901 cgacggttaa tcttgtcttg tttttgataa caatattaac gactctttta gcgggagcga
     9961 tgatggaggg ggctaatcca ttcgctcatc cactggattt gctcaaaggt gttcctttta
    10021 gtgtgactct gatggtaatt ctcggttttc acgaaaccgg gcattttctc atggcgcgta
    10081 aacataatgt tgatgcgacc ttaccttact ttattccagc gccgactatt atcggaactt
    10141 tcggcgcttt cattaaaatg cgctctccgg tgacgcggcg gcaggcttta ttggaaattg
    10201 gagcggctgg gccaatctgc ggttttttgg tagcgacgcc agctttattc attggcttaa
    10261 agctttctga agtcgtatcg ttaagtgagg caatcggtat tcaactcggt gattcactat
    10321 tgatgaaaat tgcctcggct ttgatttttc caaacctgga agtcgggcag gacattttgc
    10381 tccatccggt tggttttgcg gcttggatcg gaatgctggt aaccatgttg aatctcttgc
    10441 ctatcgggca gctggatggc ggacatattg cgtatgccct tctgggcgat aaatatcctt
    10501 atgttgcttg gggagcgtta attgcgactt tagcgctctc atttgtgtca cttaattggt
    10561 tagtgtgggg cttactagtg tttttcttgg ttagagtaaa acatccgccg atttatgatg
    10621 ataattctcc ggtgacatta cgtgagaaac tgattggact ggcatcttta gtgattttta
    10681 tcctgacgtt cattccagtt ccatttaagc aatagcatta tcgatagtat gaagcgtata
    10741 aaatacaatt ttgggcaatc agttaagcga ccattggttt atataccact gataatcttt
    10801 tttatagtaa ttctgtactt agcaatttta gtgactccat tgagttcaag taataccaat
    10861 caggtaatca ctattaaacc cggaacaaat gttcaggaag tatctgagct tttatatgaa
    10921 aaaggtatta tcaagtatcc taaacgcttt attctggcga ctaaaatgat gggaaaggaa
    10981 cggaaattat gcgccggacg ttttgatcta tatggagcta tcaattatta tgagttgata
    11041 aaaactctga gtgatctgga atcggttacg gtgcgcgtca ctattcctga gggtttgcgt
    11101 gcccgtgaaa ttgctaggtt gcttgaaaat cagattggaa tttcggccga tgatttcatg
    11161 caaaaagtta tggattccag atttactcat aaattggggg ttgaagctaa taatctggag
    11221 gggtatctct ttcctgattc ctattatttt atcgcaggtg atttgccgga aaatgtcatt
    11281 agtcggatgg tagaacgctt ctgggaagtt gtcaatgatt cagtacaggc tgaaatccgt
    11341 cgtaccggtt atactctcca ccagattatt accttagcct cgatagtgga aggagaatgt
    11401 caggtagatt tcgaacgacc gctagttgcc tcactctatt acaatcgctt gcgtagaagg
    11461 attcgtctcg aagcctgtcc gacaattcaa tacataattc cggatagtcc gcgacgaata
    11521 ctgcgacaag atttaaaaat tgattcacct tataatactt acttacacta cggattaccg
    11581 ccagggccgg tctgtaatcc cggtttcaaa tccataaaag cggctattta tcccgccaaa
    11641 accaaatatc tatatatggt ttcaaacggt gatagtacct atacccacac ttttaccgcg
    11701 aattatgagg aatttttagc cgcaaaaaga aaattgcaaa aaatccgtac ccaggataaa
    11761 cttgttgcca gtgaggatta tatcaattga aatcacttga ggatttgaat cccgaacaat
    11821 gccgtgcggt cgaatataat gccggtccaa ccttaatttt tgctggtgcc gggagtggaa
    11881 aaaccaaggt tctggcgcat aaagttgcct acctgataga aaataaaata gtcgcccccc
    11941 aaaatattct agcggttacc tttaccaaca aagccgcgcg cgaattaggt gaacgggtag
    12001 aaaaatatat cggcaagaaa gccaggcaag tcaatatcgg cacttttcat tcgatttgtg
    12061 ctcgtcttct ccgacgtgaa atagaatgtc tcggttattc ttcgaatttt acaatctacg
    12121 atgctgagga ccagctcagt cttctcaaga aaattatcag tgatcgttct attatccttg
    12181 agtccaccag tcctaaaaat gtcttgagtc gaatttccta tctgaaaaat catatgattg
    12241 agccggaaaa tttcagcgct cggcccggat ttatggctga tcgttttgta gctgtgctat
    12301 atccccttta tcagggagct ttacgtaaag ctatggcggt ggatttcgac gatctgctaa
    12361 ttctaccgct gaaaatattc cagcttaaac ccgaagtttt ggaaaaatac cgggcacttt
    12421 atcaatatat tttagttgat gaatatcaag ataccaatcg tcctcagttt ctcttaatcg
    12481 aagcgctggg taaaaaacat cagcacgtct gtgtagttgg cgacgatgac cagagtattt
    12541 atagctggcg cggcgctgat attcgcaaca tactcagttt caacgaaata tttccccaat
    12601 gccggatttt taagctcgag caaaattatc gctcgaccaa taatatcctg aaagccgctt
    12661 cagcggttgt cagccacaat aaacagcgcg ctacgaaaac actctggtca cagaaaagcg
    12721 atggcgattt attagtgaag ctggatgccg agaccgaata cgacgaagcc gccctgatcg
    12781 ccagctggat tcaacgagaa attttacaga acaaacgtaa tttcaaagac tttgccatcc
    12841 tttaccggac aaatgctcaa acccgtgttc ttgaggaaat tttacggcgc aataacatcg
    12901 tgtatgtaat tgtaggtggt gtccgttttt atgagcgcaa agaaatcaaa gatgttttag
    12961 cgtttttcaa cgtcatatcc aatccccatg atgatatcag tttaaaacgg attatcaatc
    13021 taccgccacg cgggataagt aaaacgaccg ttcaacatct tgaggaactg gctttggcta
    13081 aagacatttc gctattcgat gctatgtcgt acgccgatga actcaatctt gctacgcggg
    13141 ctatcgaatc tatcaaaaag ttttacgatc agatcaatga tctccgttcg ttacgcaata
    13201 ctctatcttt tgaagaatgg gcacgggttg ttatcgtcag cattggcttg cgtaactatt
    13261 acaaagaaag cgagggcgaa gaagcaactg aaagattagc caaccttgac gagctactca
    13321 acgatatttc agaattttgc cagactaacg aaaagccatc gctagaatct tacctcgaaa
    13381 atgtgactct ggcgacttcg attgacgaat gggaagacac taaaaatgcg gtgacgctga
    13441 tgaccttaca cggtgccaag ggcttggaat ttcctgtggt cttcatttgc ggattaaatc
    13501 aaggcttatt gccgctcgaa cgcgacttga cccatgaagc gctcgaagag gagcgtcgtc
    13561 ttttttatgt cggtctgact cgcgccaagg aaaaggtttt tttatcatcg tcccgccagc
    13621 gtatgcgcgc cggtgaagtc gcctactcat cggaatcaat gtttctcaat gaaatcccgc
    13681 cagaattgat tacaatctgc gattttcggg aaggtattat ttccagtaag cgccaaccgc
    13741 ggaccagcaa tcgttccact gctcggaaaa taaaagatac ctttgagcgg gttgaatcta
    13801 acccgatcaa aatagcggga atcaattctt cttcaagcga aaaaacttct ccgaccagcc
    13861 ggacgattga acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaatcgcaa
    13981 atttcattat aaagattatc gctatgtcct cgaacgtatt gccaggtgat tccaggatga
    14041 ttcaacctgt caagttcgag ccacagctcc tgatttttga ctgatttctt gtcagcggtt
    14101 ttccaatttc tctttttcca gttgggaagc caggttgtaa ttccattacg taggtattga
    14161 ctgtcagtat aaataatggc tttttcaccg ggttgcaatt gtttcaagcc attgagcgcc
    14221 gcgatgattt ccatccgatt attagtcgta ttttcttgat aaccagccag gactttgagc
    14281 aattgatttt cattaaaaat cagagtcgcc caaccgccgt ggccgggatt tcctgaacag
    14341 gctccgtcgg agtaaatgat tttcatctat tcgtccattt ctgaaattac gtcaatcgga
    14401 gataaagctt ccatcttaac tacctttgca ttatcaaaat aaatctgtaa ttctttgcca
    14461 tctggaaata agtaaatcca cagttcgtaa attcttccac cgctggtttc taagcgggtt
    14521 tgggaacccg gttgcccgag aatacttttt atctggtcgg cactcatacc gatgtcgggt
    14581 ttaggagcat ccggcggcgg gttgaatgtt ttacttatgg attcctgtgc tttttgtttg
    14641 gcggcttgct gtttaaggta ttcgataccg gcttctaagt ttttaatatg aggagcagtt
    14701 aagtaatgaa tatccggtcg cagagcggtc gcttgcttga gcgactccag cgctaacgct
    14761 atacttttct tatccacaga aaggttggcg tccttgatgt aaccgtcagc gatttgcacc
    14821 aaatatggct cgatgcgttg ccgaataggt ggatagtatt gaatggcacg atcgaaatag
    14881 tcaatagctt tttcccaata accatattca gtaaaaactg ctccgcgatg gaaataaatt
    14941 tcactgtagt agcagtagat aagctcatta gtgtttggga gattcagctt tgcgattgtg
    15001 tttaaaaatt gttcggcttc atcgattcga ttgttgctaa tcatcacgtt cattgtatca
    15061 acaaactgtt taatcattct ttcgtaatta ctatttatcc gagctaatag ggttttgtct
    15121 ttagtcactt gggctcgcgc taaatattca gtagctatgg aatagttttg acgggcaata
    15181 aacaattccg ccagagttac ttcctgatag ggaattcgct tgtcgcattc tttaatcatc
    15241 cgacgggctt taaagcggct tgggtggtgg ggattatcgg cgatgaattg ttcaaaggtg
    15301 gctttggcgg ctataaaatc attagcctga taaagagctt gggcttcgtc accagtactc
    15361 cgacctccgc cgagattaga attgaatgtc agagaaatcc ccacagtatt aaaattaatc
    15421 ttcttaatcg ggcttatgtt atgagggtct tccaaatcac cgaaatcacc gactgtatat
    15481 ctgatttcta cgccgtaacc ttcttttaat ttataattga tactgccgag gaatttaaat
    15541 cccagcgcga aggagtaggt gtgccctttt tgatgtagaa aatagtcgtt agaatgagtt
    15601 ttataagctg agccggaggc ttgaccgtaa ctgatcgtta agtaattata aaagttcggc
    15661 gcccattgat atatcaccgt ttgattgagg ttgaattcat agattcgggg tgacaaaaaa
    15721 agttggtcaa cggagcccgg cagacgttgc ggccaggttt ccgggagttt ttgttcggat
    15781 aatgagtatg tgtatttagc gcctattccg gtctggaaat cgagcaaatt ttgcggcaaa
    15841 atatacacca gccagttgaa cttcaacaaa tccagttcaa ggctgttcat cgttcgttcc
    15901 caagaagttt tatagccgtt aaaggaaatt ccgggggaat caaatagaag tggctgaccg
    15961 tcaaatccaa atgtctgtat gccggtttta aattcgaatg gtgtcaattt aaatggtgtg
    16021 cgatattcga tcggtccgtg aatagcatcg acaatcttga aataccattg tgcggataag
    16081 ctaacggtta ataccccgat tattaagata cattttacga atcgagccac ggatagaaaa
    16141 taacaatacc tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggacattt
    16261 attgttattt tattggcggt cttaaatctc gccgggcaga gtaagaatct ctatggaaaa
    16321 tttacgattg ctcgtctgaa atatgacggc ggtggcgact ggtacagcaa tccgagttca
    16381 attccgaatc tgttgaaatt tctccgtgaa aatctcaata tggacgtcgc ctccgacgaa
    16441 gtgcgggttg gcataatgga caaagacttt ttcgcctatc cttatcttta tatgaatggt
    16501 catggcaacg tgcgtttcag tgatgaggag gttctgcgcc ttcgccagca tttgcaaaac
    16561 ggtggctttt tacatgccga cgataattat ggtatggatc aatcctttcg gcgtgaaatt
    16621 aaacgagttt ttcccgataa tgacctggtg gaattgccgt ttgatcaccc gatttataat
    16681 attttgtata aatttcccca cggtttgccc aaaatccacg aacacgatgg caagccaccg
    16741 cagggactgg gaatatttta caaaggtcgg ctggttgtgt tttatactta tgagtgtgat
    16801 ctcggcgacg gctgggaaga cccggaagtc cataatgatc ctgaggaaaa acaccaggcg
    16861 gcacttcaaa tgggcgccaa tatagtttta tattcgttga tgcaataaat gggaaataat
    16921 caatccagct ataaccggat cgaacgcaaa ttaaatgact tgcgacgcca atttgcgcgg
    16981 catgttttac aggtcggtct gctgaaagtt ttattgttga ttataatcgt gactttttta
    17041 attgttgtcg tcgaaggttt tcgctatttc aagggttcgg ttcgcagtga cattttattt
    17101 tttacggttg gattcagcct catttttctg gcgattccaa ttgtgtatta tttccaaata
    17161 atccggaatc gaattagtgc ttacgatgat tatcacctgg cacgccggat aggcgccgac
    17221 cagccggaaa tcaaggacga tttactgaat gccttgcagt taagggaatt gctaaataaa
    17281 gaaacgcgcg gttattcgag tgaactgatt gaacaagctc tggagcggat agcccgaaaa
    17341 atagagccgc ttaattttga tcggataata ccatctcagg aagtccggaa gagtttgcgt
    17401 aatctggcaa ttgcgatttt ttgcctcgta atttgtatcg cctccagtcc cgcatatttc
    17461 aaaaatagca cggaacggct tttgcatccc agaatcgaat acccggtcaa attgcccttt
    17521 tctatcgaat caacccgagc gtcatcgggg gtgctaggtg gcgattcggt aacaatcagc
    17581 tttcagtgtc agggacgatt tccgcactat ttaacgcttg accttcagta tccggattac
    17641 actcggcaag agattttaac ggtagatgat tccggacgga gccaatatct gatcgaaaat
    17701 ttgcggcatg atctggttta tagcgcttat tatcaaaatc attcgccgtt caagccctgg
    17761 cgccgaatcg gaactcctcc cgatacaatt cgagtcatca accgacctga aattctctat
    17821 gtcaaatccc gttttattta tccggaatat accggacttc cggctcagac tcaggaaacc
    17881 aaaagcgcgg aatttaactt gctggctggc acgaaaatat ctttagaaat tcgtataaat
    17941 aaagaaatta aagaaggcca tttatccttt gccagcggga atgtcttaaa tttggcgacg
    18001 aatggtgatc gcgctcgtgg agaattcatc gtaaaaaatg atgaccagtt cgaaattatt
    18061 gtagccgatg aaaagcaagt gacaaatatt aatcctatca agtaccgact gagcgtcatt
    18121 ccggatgcct atccgactat cactatgctc agcccgaaaa atgatcttga tttaaacgcc
    18181 gatatggtaa ttccactcgg tattcggatt agcgatgatt tcggattcac ccgcgccgct
    18241 attcgctacc gtgtgataaa aaaatatgcc gattatcagg aagaggaaac ggaaataaca
    18301 ttcccactcg caaaattgaa tatcacctgg caggaagtat tttatccctg gcaaatcagc
    18361 gaacttagat taggtccgga agatgcagtc gaattccggg ttgatattta tgacaatgat
    18421 aatattagcg gccccaaaaa agccagcacc gactggctaa tcgcccgctt tccatcactc
    18481 aatgaacttt ttagctcggt caacgaacaa caggatgaaa tttattccag tagcgaggaa
    18541 gttttaaaat ctctggaaac taccaaaaag gttctcgacg agatttcccg tgaattactc
    18601 aaaaaaccgg aactgaaatg ggaacagaaa aagcaattag aagaggagat gaaaaaaact
    18661 cgcgaagccg gtgaaaaaat caccaaacta agcgagcaac tcgatgactt gatagagaaa
    18721 agtcgtgaga atcaactctt cgatgcggaa acattggaaa aattagccca ggtgcaggaa
    18781 gcatttcagg atataatgac accggaaatg cgcgaagcac tcgataaatt gcaagccgct
    18841 atcgaaaaaa tggatccaaa ggaagttcaa aaagctctaa ataatttcaa aacttcccag
    18901 gagcaatttt cgcaggaact cgaccgcctc ctgaaaattt ttcgccggat tcaaattgaa
    18961 cagaatattg atgaattggt taggcgcttt gccgatctga gcgaaaggca gaggcaagtg
    19021 gctaacgagc tgaggaaaac agcgccgaat gatcagcaaa aattgaatga attagcgcag
    19081 gcagaaaatg ccattaaaaa tgatactgaa attgctcagg atattatgaa aagaactgcg
    19141 gctgatatgg cagaatttcc aatgatgccg aataaagagc ttcaacaatt gatagatgaa
    19201 atgcaagcga tggatctacc gcaagaacag tcgcaagcca gcagtgccat gcgcaaaggt
    19261 gataaatcgc aagctgagga gtcagcccag aattctcagc aaaacttgca gaaattgtta
    19321 gaaagaatgg aagaatttcg cactgaattt cagagacgaa atatggcgga cgttatgaca
    19381 gacttccgtc ggattttgaa taagacaatg caattatcct atcgtcaaga agaactcacc
    19441 gaggagatta aaaacactcc gcgtcaaagc gatcagatta tggatatagc cgttaagcaa
    19501 agtgaaactc gccagaattt gtcccgcctg atttccgatt tgattgattt gtcgggtaaa
    19561 acttttggcg tttccccgcg tatcggcaaa aacctcggtc aatcagctaa tagtatgaat
    19621 gcggctatcg cctatatgga agaacgagat ccgggactgg cggcactttc cgctgaaaat
    19681 gccaccgctt cgctcaatat gacggctctg gaactgatgg cggctatgca agaattacag
    19741 aagagcggtt cggcttccgg tttcgaaaat taccttcagc aattgcagga attggctggt
    19801 atgcaacagg gtttgaatga agaaacccag atgctgggat tgggttctcc cggtgatcag
    19861 ggaactctcc agcaattagc cgctcgccag caacaaattc gccaatcact cgaaaaatta
    19921 cgcgatgaga tgcaagggca aggtagcact aaacagtcgg gcgatatcgg tggcattgcc
    19981 aaagatatgg acgaagtcat caaagacctc gagaataacc aggttttacg ccgcacgctg
    20041 gaacgccagc agagaattct ctcccgactc ctggatgctc agaaatctct gcgcactcag
    20101 gattttaaag aggaacgcca gagtaaaacc gggcaggaaa tcgttcgcga atctcctgat
    20161 ggtctgccgt ctaaccttgg cgagcggcgc actcttttac aggaaaatct agaaaaagct
    20221 ctgcgcgacg ggtattccaa agaatacgaa aatctgattc gccggtattt tgaagaattg
    20281 agtaaggagt cggtagaatg aagctcgaat ggcgtttggg attattaatc ttattaattt
    20341 tttggcttac agctttcgtt gccgctcaac cggtaaaagt cgtttcaccc gaagaaaaac
    20401 tgatgaattt acgctttaat caggctcagc gcctcgagca ggaaggtcag ttgacgcaag
    20461 ccgctgaaat ttaccgggcg ttggttgatg cccaaccggc taattatctg tattatagca
    20521 attatgtcaa cttgcttttc cggcagaaaa atttaccgga gctcgaacgt gtcattaccc
    20581 gttttatgca attgaatccg cttcatgaaa atgctgctat tgatctcggt aaacttttct
    20641 atgtgcgcgg tgacagcgct gaggcgtttc gggaatggca gaaagccgtc gtgaaattta
    20701 actacagcgt ttcgttttat cgtgctctgt tcaatgaaat ggtcggcttg agattatatg
    20761 atgaagccga gcgcttgatt gccgccgccc gcgctcatta tagacaaccc gatttattga
    20821 cgatggaatt agccaatttg caaattgcac ggggaaaata tagctcggcg gcaagagaac
    20881 ttgtacttta tgctcgcgct aatccgcgtt attacaatat gattgccgga caaatcttgc
    20941 gtttcccgac tgactcgagc ttgtttattc agatagattc cttgctaaag gcggagttgt
    21001 tacttttgcc cgatcaagcc gctctgcatc gcctgcgggc ggattttctt tttaaaaacg
    21061 ggcattataa tgaggcactg gaggaaattt tccaggttga agcgctgact ggtaatcatg
    21121 gagatcaagc gctggcaatg gctaaaaatt tagtccagat tcggcgttac gaactcgccc
    21181 aggattttta tacgaaaatt ttggcaaaga acgaattgca gggtatcgct ccgcaagctt
    21241 tactcggatt agctgagtca ttcgagaaag ctgggctgga acagacaacg gctgagccgt
    21301 tgcattattt ttatcccggt aacttctttt tcaataccga ctttgtccag caaattaatc
    21361 aggaacaatc gagtttgcag aaagcctttg cgatttatga ttctttagtt gccaatcaac
    21421 ccaatagcat ctattcagct caggctttgt atcggctggc agatttgcgt tttcgggcag
    21481 tgcgtgattt tgatggagcg ctgaatttac tgcgtcaggc tcagaatatc acccgcgacc
    21541 gacaattaat tttaaactgt gctttacgaa taggcgaggt gcagattgcg cgcggcgatc
    21601 ccgacgcggc tcttaaatat tatcaagcag aaattgtacg atgggaggga acaccaggtg
    21661 aaaaagattt acgagttcat ctggcactag ctcaattttt ggcgcaggaa tttgattcat
    21721 tggacatcga actgaaaaat ttaataccat tgctgggacc aaagcatgcg ctttttaatg
    21781 acgtagtcgg ctttgatggt tttttccggg caaattatag cgagaccgat gccgccggga
    21841 aaaaagcttt tagtgaattc gctacggctg aacttctttt acgccaaaat aaattgagtg
    21901 aagccgaaaa gatttatgcc tttattttac aaaactatcc tcaaaccccg atcatttcag
    21961 ccgctcggtt tcgtttaacc cagattcttt tacagtttaa tcgttatgcc gaagccgagg
    22021 cgaccgccga atcctttttt gcttctgaaa atgaaaatgc cgatcaaatc gcttttatgc
    22081 tggctgaagt tgccgactat cgtgaggcaa actatcaagc ggcggcttac tattacgaat
    22141 taattcttga aaaatatcct aattcatttt taattgataa tgttcgcaag cgtctacgcg
    22201 aactgcaacg cttgtcaggc gtaaaaaaag aaatttgaat cgaatgaagc gcaaccggcc
    22261 tttaattgta ttaattggac tgattttaat cctggcgaat ctgagttttg cccaaaaaat
    22321 tctgattccg atggatcaaa ctcagagcga tcacctaaaa gcttacggca ttgtttactg
    22381 gagtttgaag caaggagtaa atgtcgagtg gttgttgaat tatcgaggcg gctcttttct
    22441 tctggataat tatccaggca ttgttaaaga actacgctta cgaggagttg cctatgaaga
    22501 agttagcgcc gctcaaaacc tgcaaattta tgcgacgatc gaagaaaata atatggacgt
    22561 tgtgctatta gaaaaagccg ctaagattgc ggtttattcg ccacccaata aacagccttg
    22621 ggacgatgcc gtaactctgg ttctgacgta tgccgaaatt gattacgatg ttctctggga
    22681 tgaagaagtg ctaactggga aattgccgga atatgactgg cttcatctgc atcacgaaga
    22741 tttcaccggg caatacggaa agttttatgc gagttatcat aatcaagctt ggtatcaaaa
    22801 cgaagtcgct gagaatgaag ctatcgccgc taagttaggc ttccgtaaag tttcagagga
    22861 aaagaaagcc gtagcgcgta ctataaagaa atatgtcaat gaaggcggtt ttttgttcgc
    22921 gatgtgttcg gctaccgatg ccctggatat cgcattggcg gctgagaatg tggatattgt
    22981 cgccgaaatt tacgacggtg atccggctga tccgcgcgcc aatgaaaaac tggattattc
    23041 caagtgcctg gcatttgaga attttaaatt gtatctggat ccgatgctct acgaatattc
    23101 cgatattgat tatcctccaa gctttcaagc cgtagcgccg ggtgccgaag ccgattattt
    23161 taccttattt gaattttcag cgaaatggga tccggtgcca acaatgttga ctcaaaatca
    23221 tgtcggagta ataaaaggat tcatgggaca gacaaccgga tttcgccgcg atcggattaa
    23281 aaaatcggtt gtcgttatgg gtgaagttgt taacggtgag caagtgcgct atatccatgg
    23341 taatcatggc aagggaactt ttacctttct tgccggacac gatccagaag attatcaaca
    23401 ttttgtcggt gatccgccta cccggttgga attacataaa aattcaccag gttatcggct
    23461 gatattgaat aatatacttt ttccatcagc ccgtaagaag gaacgtaaaa cttaagagag
    23521 gaataaaaat tgaaagaaat tattttaaaa accaatcgtc gtatcgagct aattgacatt
    23581 acagctcaag tggctgaagc tgtccgctcc agtggaatcc gggaaggaat atgtgtcgtc
    23641 tattgtccac acacaactgc cggtttaact atcaatgaac atgccgatcc aaatgtcgcc
    23701 agcgatatcg tcaaccgcct aagtaaattg gtgccccaca atgaaaatta cgcccatatc
    23761 gaaggcaatg ccgacgctca tatcaagtca actttaaccg gcaatagtgt ccagctgatc
    23821 gtgaataatg gaaatttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca
    23941 gcaaaggcaa tggcgaatag taacatcgtt tgagtcaggc tgttccaact cgcggcacag
    24001 aacaggatag agtgaccaac taattgggtg ataactccca taaggaaagt caatactccc
    24061 agaacgcctg aaatcaatac taaattcttc ataaaatcct ccgtatttaa tattgtaaaa
    24121 taccttgcta atattaatag tgttttatca gaaagtcaat tgtttattat taaaatagtt
    24181 gcaataatca gattgtagtc gtggtttttg ggtgttttct attttaatca attaaaaaaa
    24241 caagctccag atagcgccag cgcgacaccc tcaatagaaa gttcaaatga ttccgaatcg
    24301 ttgaatatga tgactaaatc agccaaaaat attcagaatg tttaagtgaa aatgacgata
    24361 tttttattgt ttattttttc taaatattcc atatttaatt aaagaaatat ggtagcgatg
    24421 agtcggagac ggaaatcaat aacaaactta attctgatct tccttctaat tggaatactt
    24481 ttttatttac attttcgcaa ccttaggact aaaataacta aatctaaaaa caatgttcca
    24541 atagaaaggg tcgagcaatg aagctaaaaa atgaacgatc agttttagtg attattgatg
    24601 tacaaacgaa gcttttatcg gttatgtaca attatccgct cttactcgag aatcttaaaa
    24661 aactaatccg cggttcgcag gttctggata taccgattat ctatactgaa caatatccaa
    24721 ccggattagg gccaactcat cctgaaattg cctcgttatt atcaaatact ccaccgatta
    24781 taaaaatggc attcagttgt tgcagtgaag aaaattttat gaatgaaatc cacaaaatta
    24841 accgcgatca aatcatcgtt tgcggaattg aatcccatat ttgcgtttac caaacctgcc
    24901 gggatttgat tgataaaaag tatgaagtgc atttggtaac cgattgcatc gcgagccgca
    24961 agctggaaaa tacgcaactg acggtaagca aattgctggc ttacggggtt caccccacga
    25021 gcgtcgaaat ggcactcttt gagatgctga aagatgcatc gaatgacgcc tttaagaaaa
    25081 ttgctaagat cattaaataa aggttctgcc aaatgaacat tgaacagatt caaaccgaaa
    25141 tccgtaagca gaaagttgac gggtggcttt tttatgattt tcataaccgt gacaaaatgg
    25201 cttaccatat tcttggctta aatccgaata tgttcgtcac ccggcgctgg tattatttca
    25261 ttccggccat aggcgccccc ataaaacttg tttcggccgt cgaagcgact aaaatagata
    25321 atctccccgg tgaaaaaatt atctatcgca gttgggaaca gcaacatagt agtcttcgca
    25381 ctatgctcaa aaacagtcgg cgaatcgcga tgcaatattc gccgaataat aacatcccct
    25441 acatttcggt tgttgacggt ggaacgattg aattaattcg ctcttttggg gtggaaattg
    25501 tttcttcagc taatctcgta caaatttttg aggctgttat tgatgaagaa ggctatcggt
    25561 cgcacctgcg agctggtcag aaagttcagc agataaaaaa tgaagccttt gccttaatca
    25621 gttcacgaat aaaagagcaa aaatctgtta ccgaatttga agttcagcaa tttatcgtca
    25681 aacgtttcgg cgaagaagat ttgacggacg acaacgagca tccgattgtg ggagtcaatg
    25741 aacatcctgc cgacccgcat tttgagccga aggcggagaa tgcttacaca ataaaaccgg
    25801 gcgatacggt attgatagac ctctgggcgc gcgaacaatc cgaacgggca atttattatg
    25861 atattacctg gtgtgggttt gttggccaaa atccgcctgc taaatacgtc gaaattttta
    25921 atatcgtggt gcgcgctcgc aatgcggctc gtgattttat ccgtcgcaaa atggctgaca
    25981 accagccttg ttacggttat caagttgacg acgcctgtcg gaatgtagtc aaggaagccg
    26041 gatatggcga ttattttgtt catcgcaccg gacattcgat cggacattat gtgcatggca
    26101 atggcgtcaa tattgacaat ttagaaacaa aggacgagcg cgaactggtg cccggtatct
    26161 gcttctcgat tgaaccggga atttacctgc cgggtgaaat ggcggtgcgg tcggagatta
    26221 atgttttcat aaccaaagac cgggaagtgg tcgtcgctgg tgatgaacaa gaaaaattgt
    26281 tattattata aatgacaatg aagtctttaa caaacaatgg aggttaaaaa tgaagaagac
    26341 gcttgttgtt ttttgtgtgg tggtgatttt gattttagcg agtctggcga ctgccggcac
    26401 gccgacgacc cagaaaggtt cggcggctat gtggtttaat tttaacggat tgagcgatat
    26461 gagttaccag aattccgcga tcgggctaaa ttacttgttt aaggacaaaa ctggtgccgg
    26521 gatagccttg aatttttccc atgatagtga ggatgatgga atagaagaga aagaaaaaac
    26581 tactgccctg ggtatggatc tatacttaat ccattaccca ttccagaaag gtccggtggc
    26641 gttatatctt gctccgggtg tcggatttaa tttttctagc aaaaaagata aagattttgg
    26701 tgacattcat atcaattcga cttatatgtg gttaggtggc tcggtgggtg tcgaatggtg
    26761 gattttcgat caggtgagct tatcggccag taattgggta ggtctggaga ttgacttatt
    26821 aaatgataag gaaaatgatg ataagatgac tgacgtccat ctgggaattc tcggcagttc
    26881 gacggcgagc atcgtgcttg ctttctattt taaataactc tgcagtcgtt aaaaatttag
    26941 attcaggaaa ggttaatgaa ttcattaacc tttcttattt tgtaacgtga gccaattcat
    27001 cgagaatatt aaatgcttcc tgtacggatt tgcttcgtac caatttttcc cggatttcgg
    27061 agacgtgtcc gaagccgcgc aaataccagc ggtagaattt tttcatttct ttatgagcta
    27121 cgacttctcc gcgatattgg atctccatct ctaaatgtcg gcgacagagt ttgactcgtt
    27181 cggtgggcga atcataggaa attagcggct tatcttccag taaatctgag atttggcgga
    27241 aaatccaggg attttcgagc gccccacgtc cgatcataac cgcattgcaa ttggtttcag
    27301 cgaacattct taaagcagcc gctggtgtaa agatatcgcc gttaccgata atcggtaaac
    27361 tgattttttt ctttatttct ttgatgagtg accaatcggc tggatggcga aaaccatcaa
    27421 cagcagtgcg tggatgaatt gttatagctt taatcccggt ctgttccaat cgctgggcaa
    27481 tttcaacagc gataatggat tggcgatccc atccgctccg catttttacc gtaaccggaa
    27541 ccgataccgc ttcgacaacc gcacgggcaa ttttttccag taaaggaaga tttttcagca
    27601 aggcggaacc ggcgtttttc ttaatgacct tgcggaccga gcatccggca ttgatgtcaa
    27661 taaggtcggg atgaaattca ttctctatca aattggcagc ttccgccata accacgggat
    27721 tgttgccgaa tatttgcata ctaatcggtc gctcgaagtc ggaaaaacgt agatagtcat
    27781 aagttttttc tgaactgcgt accagaccgt cactactgac gaattcggta caaaccaggc
    27841 cggctccatt cgctttacaa atctggcgaa aagcttggtc ggtatagcca gccatcggcg
    27901 ctaaaatcag cggcggcttg atgcaaacct tgccgatagt caaagcagta attttttgga
    27961 cagaatccat aattttccat tttaatcact attactcatt agcatttcat ccaaaccgtc
    28021 gtcaaccgct aaaaaatttg ttttcaaaaa tgaacgggct agcgaaatat ctaaacttaa
    28081 atcgcgtggt cgcggcgctg ttaattgatg ttcgtcgtat ggaacggctt ggataaaagt
    28141 caggggtaag tttagcttgc ggcaaagttt ctcgccgaag gcgaaccggc tgatgcgttc
    28201 cggaccgccg atatgtatta ttccacaaaa atgtgataaa gccagctcga ttaatgcttg
    28261 agcggcattc gtgaccatca acatactgcg gtattgatcg ttataaagtt tcaacggtgg
    28321 taatttcggt tcttttttgc gccgtagtag ctctcgtagt agccattctg aattgtaagc
    28381 acgcggaaaa attccgcgac cgtacatcaa ggccagacga gctacgaccg tatcgggatt
    28441 atttgccatc gcgagttttt cggcttcgta ttttgatttg ccataaacac ttaacggatt
    28501 aggtcggtcg gtttccgaat aattaccttt ctgaccatcg aataccatat cggttgaaac
    28561 ataaatcaaa cgggcattat ttttatgaca ccaactggcg atatgttcgg tagcgataac
    28621 attattttgt ctggcaaagt ccggttgatt ttcacattga tcggggtttg ccaaggcggc
    28681 gttatgaatt acaacggcag gtttaaatcg tttgaggaca acgatgattg aactcggcga
    28741 ggaaagttcg attcgctggg ttgtgattcc ttttaaattg aaaggataag tcgaataaag
    28801 tccgagtgtt tggaaacgag aaaccgctaa atttgcaata tgtccaccca ggaaaccact
    28861 ggcgccggtg atgactaatt tcttcatatc ttttgccaga tttcgagaat caaactgcca
    28921 atcagataat cggcgtcaat tagtccaaaa gaaggtaaaa ggaaatccat tttcattgtg
    28981 ataacgagtc agttattaga ttaagatagc gacggtcgat tacccgatat gctggttggt
    29041 aattttttat atattgactt gaatgatgaa tcaccgggta gccggtcttt ttgatactat
    29101 cggaaaaagt gaaaaaatct gaccgcggaa taggcaatga gccttccaca actaattttt
    29161 cgatagtttc ccagcggcga atgaatctgg aggaatcggc tgaagattgg gggattgagt
    29221 tcaagatggc tatgacgatt ttggcagttt ggatttgctc ggctttacag cgttttaaat
    29281 ttaggcgcac atatttaccg gaaggtgtga tgctttccga taacggggcg ctcgaatccg
    29341 gtgtaataat cgccagctcg ttttccagac cggttgatat ttggattgag tcgttaccta
    29401 agtgcgccgg tccgaaagtt ccttggtaaa ctaatttata tacatcggcc ggttgcattg
    29461 ccgggtagcg attcaaatgt tctgcgatca aagtgcgcca accggaaggt ttgtcatctg
    29521 gagtttggcc gaaaataaaa aagactaata tcaatacaga cgaaataatc aggatgttta
    29581 agaaaaaaac ggaaatagtc aaatttgcca gtttatcaat cttttggttc tttttctggc
    29641 tcttctttga tttctctaaa cttggcatct tcgatttggc ttttatcaat tttaagggtt
    29701 gtgcgtggtt ttttaggttt gggattattc ggtttaggct gtcgtagatt gcgcggcttg
    29761 accattttcc aaagcaggta aaagatcaga aaaagtagta atagtcggag cattataaaa
    29821 tcttcttcgc tggattataa taaatcgtcc cgctaattgg ttgcttgatt tcccggatca
    29881 gttcattcag atcattttca ttattgacga agtcaatttc attggtgtta acaataatta
    29941 gcggactgtc tttgtagcgg aagaagaatt cattgtaaat ctggttgagg gaatcaatat
    30001 agtcgcgatt gatagatttt tcataagaac ggccgcgttt tttgatattt gatatcaggc
    30061 ggtcagtgct ggcttgtaag taaactacta ggtccggttt gggcaagtct ttttccaaat
    30121 gttgagcgac caggttgtaa agggacaatt ctttttcatc gagattgagg attgcgaata
    30181 gtttatcttt ggcaaacata taatcgctga ttaaaagatt agtgaataga tcaatttgtt
    30241 tcaattcgat ttgttggcgg tagcgactca agagaaaata gagttgagct tgaaaagcgt
    30301 atctttccgg atcgcggtaa aaatcttcca gaaatggatt ttcctcgaat ttttcgagaa
    30361 ccagacgagc attgagtttt tcagccatta gacgtgccaa gctagtttta ccggcaccga
    30421 taacgccttc aatagctaaa aagtaaaggt tcttcataga actccagtag taacgggaat
    30481 agtcagtaat tcatccagtt ggccgattct aacaacttgc tcgatatccg gacaaatcct
    30541 tagcaactct gcgaccgatt tagcggtttt ggggcaaatg aattctgggg cgatttctgc
    30601 cagtggcaca agcacaaatt tacgttcgta gagacccgga tggggaattg tcagagtggg
    30661 gctttcaact ataagccgat taaaaaagat tatatcgagg tcaatttcac gaggcgaatt
    30721 cttaaagtgg gggaaatgcc ttcctaatcg aacttcaatg tctttacaaa attcgagtaa
    30781 ctgtaacgga gtcaaggtgg tggcaatttc tactaccata ttcaggaact tcggttggcc
    30841 gggattgtat tttggggcag tttcatagac cgctgacata cgacagatgg aaattgcagg
    30901 attatgcgac agtaattcca gagcggctga taaatttttt tcgcgcaagt tcaaattact
    30961 tcccagagcc acaaagacgc cagaactttt cacgattttt ctcccatttg gtcgcgtcgt
    31021 cgaaatattt caacttcgac cgtatccagc acgcccttga tgggcgcatt gggtttgcga
    31081 atgcgaacgc ggaccgattc taccagatga tgattaaaga tttcgcttaa aattttaccg
    31141 gctaatgctt caatcaggaa aaatttggac tgggttacga ctccctgaac taactgataa
    31201 accttttcgt agtcaatcgt gtctgctaga tgatctgtgg taatgacctt agtcatatca
    31261 aattccagct cgacatcaac ctcgattttg ccgccgagta ttttttctgg ttcggctact
    31321 ccgtggtaac cataaaagac catgttgttt aatctgacaa tacccataat ttttcaattc
    31381 tttagactaa tggaatttag agaatgagtg tcaaaaggac aagaaagtaa gtcatattta
    31441 tgattaaatc ctttgattga ggaaatatat gataggtcaa tttgcccctg gatggtttgc
    31501 ccttctggcg tgaatctgtc aggaaagaat gccaattcta ccaaacatcg ccacatcggc
    31561 aaagtgcttt gtcgtttttt gcaaatagtc tgacaatatc cggtgttttt tttgggtcgg
    31621 catactactt gagtaattat taagcggttt ttgaaaaatt gaaatgagaa agtaaaaaat
    31681 aataaaaaaa ggaggtagaa atggcaatta ctaaagcaac tccaaaaagc gtaacgagga
    31741 aaagtcctta taccattgat cggtttttcg atcctttctt cgattgggat acagaaacat
    31801 ctttagtgac ttttgctcca gaagttgata ttgaagaaaa agatacggaa tacgttgtta
    31861 ccgttgaact gcccggtgtc aaaaaagatg atgtcaaaat tagcctgaaa gataatgttc
    31921 tgaccatttc cggcgagaag aaacgggtta aaaaaatcaa caaaaagaat ttctatcaat
    31981 gtgaacgcgt atttggttcg tttgaacgtt ctttccgctt gccggagtta gttgaccatg
    32041 agaaaattga agctgagttc aaagatggta ttttgaccat cgttgttccg aaatcaccgg
    32101 aaagcaaacc gaaatcgatt gaaataaaat aaacaaacaa actaatctaa aaagaaagga
    32161 ggtgtgttat ggcaattact agatggactc caagaagctc gtggttggat gtaaggaatg
    32221 agtttgaccg tctgttcgaa tcaatcttca atccgcagta tgaagaggaa accagtttat
    32281 tagcattcag ccctgccgtt gatatcatcg aagaggataa ggcttataac atccaagttg
    32341 aactgcctgg tatgaacaaa gatgatatca aaatcgcagt gaaagataat tacctgacta
    32401 ttagcggcga gaaaaaacgc gaatctaaag tcgaggataa gaattatcaa cgcactgaac
    32461 gcatctatgg ttcattccaa cggaatttcc gactcgatga ttcggttgag gctgataaga
    32521 ttgaagccga atttaaagat ggtgtattga acatcgtcat tcctaaaaag aaagaatcac
    32581 tcgccaagca gattgaagtt aaggtcaaat aatttcctcc caatcaccct caatcgagag
    32641 aagtccggct cttagccggg cttttctttt ttatagactt gccagatttt gtttatcaat
    32701 ttaaatcaga ccttgtagga taaataccga caagaaaatc aggctttgtc tgatttacat
    32761 gtcggagtga ttaacctata tttaatcatg aattatatga atcccacacc ttcagataaa
    32821 atattttaag catgaaagtt tttatcgccg atgattccga aattctgcgt aagcatttaa
    32881 cgaatctgct aacagaaata ccaggggtgg aaattgtcgg ctatggtgag gataccgaat
    32941 ctgctattag cggaattgat acccaaaagc cggatgtagt tatcctcgat attcgtatgc
    33001 ctggagccgg cggcatttat gtgcttaaaa cggtgcgcca aaatcgtcct gatttgcgta
    33061 taattgtctt taccgattat ccttatcccc agtatcgcaa aaagtgtctc gaagaaggaa
    33121 ctgattattt tttcgataaa tccagcgaat cagaaaaatt aatcgaattg gtccgaaaaa
    33181 tggcaggggg taaagcctga atcagttttt aaaaaatact ttatggattg agtttttcag
    33241 atggaggtct attgatgata attgaaaaag ccgctttatt cgacttttta acgcgctgtc
    33301 agaaagaaca tctgggtgat ttcaagcaag tgattgcacc gcgtcgttcc gataaatccg
    33361 atgatgttct attttttgat aattggcggg aaggggcaga atttgtgatt gacagttatc
    33421 g
//
LOCUS       DAOOMU010000012        33369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1092_length_33369_cov_17.113526, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000012 DAOOMU010000000
VERSION     DAOOMU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 33369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33369
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1092_length_33369_cov_17.113526"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2534)
                     /gene="alaS"
                     /locus_tag="PLI55_02110"
     CDS             complement(<1..2534)
                     /gene="alaS"
                     /locus_tag="PLI55_02110"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPA99664.1"
                     /translation="MKTANDVRREFLEFFASHGHKIVRSAPVIPQSDPTLLFTNAGMN
                     QFKGIFLGQEKIESPRIANSQKCIRVSGKHNDLEEVGKDTYHHTFFEMLGNWSFGDYY
                     KAEAVQMAWELLTEVWKLPTERLWVTIYQDDEEALQAWQRIAGLDQSRILRFGEKDNF
                     WEMGETGPCGPCSEIHYYTGPHPEHQQAEMINSGNPEYIELWNLVFIQFDRDKSGELK
                     PLPLKHVDTGAGLERMVAVLQGKKSNYDTDLFKPIIDRIVSLTGKEYHTENGVPHRVI
                     ADHVRMLTFAIADGGLPSNDGRGYVIRRILRRAARFGRMLEMHKPFIYQLVDSVIEVL
                     GATYPEIAERRNHIQAVIKAEEKSFGLTLDRGLEVFAKLKENLQRNNITIIPGEEVFK
                     LYDTFGFPMDLTRLLAEEAGLSIDEKGFENLMNRQKERARASVVFQADDFLENEDWQT
                     LTTGDDSEFVGYENLETQSVIRKYLIEGQKVHLILDRTPFYAEAGGEVGDVGIIEGNN
                     FRIRIDNTRKIGSSIIHSGHFEKGNLIDNPMVKASVDNVANLQIRANHTATHLLQAAL
                     RQILGEHVHQSGSLVTPEKLRFDFTHFTKMTKSEIEAVEDWVNARIRENLPVNAEVKD
                     YQSARESGAMALFGEKYGDVVRVINVGDCSKELCGGKHVNRTGDIGCFKIISETAIAA
                     GVRRIEAITGEAVLQLMRENERLLEKITTILGVSREQLPQRIEKLLTDYKNLEKQFHK
                     NNKADLEHEIDTLLEKAEMIGELKVFTYQANVKSLAELKEIGDLVRAKLHNGVGVIAG
                     IIDEKPNITVVITDHAIKRYGLKAGDIVRELGKILGGGGGGQAHLAT"
     gene            complement(2680..3333)
                     /locus_tag="PLI55_02115"
     CDS             complement(2680..3333)
                     /locus_tag="PLI55_02115"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HPA99665.1"
                     /translation="MSSDFSSEAEILKVVTKIESQKKKSARINIYLDNEFAFGISAET
                     LNLFRICVGQQLTEKQINMIQQHELFEQAKEAAIRYLGLRIRSQRELELYLTRRQKYP
                     LTVASRVIKYCTERGYLDDKVFCETFIRDQLELNHNGIRKIRQALVAKGIDREVIDEA
                     IKNLVKDEDQLKAAWIIGQKKASTLKNDPKRRDKLYRYLIQKGYKPDVVIKVLQKIL"
     gene            complement(3361..4368)
                     /gene="recA"
                     /locus_tag="PLI55_02120"
     CDS             complement(3361..4368)
                     /gene="recA"
                     /locus_tag="PLI55_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPA99666.1"
                     /translation="MAEKNDDKIKALDLAITQIDRQFGKGSIMRLGDDSMKITVDVIP
                     TGCLSLDACIGVGGIPRGRITEIFGPESSGKTTLALHIIAQAQKAGGYAAFIDAEHAL
                     DPQYAAKLGVNTRDLLISQPDHGEQALEITETLVRSNAIDVIVIDSVAALVPRAELEG
                     EMGDSHVGLQARLMSQALRKLTGAVSKSNTAVIFINQIREKIGVTYGNPETTTGGRAL
                     KFYTSVRLEIRRISTIKEGNTNVGSRTRVKVVKNKMAPPFKETEFDLTFDQGISYVGD
                     ILDTAVEAGFVQKSGAWYSYGDERIGQGRESAKGYLTENKELLTKLEQQVKTYLGLIK
                     E"
     gene            complement(4384..4941)
                     /gene="thpR"
                     /locus_tag="PLI55_02125"
     CDS             complement(4384..4941)
                     /gene="thpR"
                     /locus_tag="PLI55_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPA99667.1"
                     /translation="MKRTFIAIPVSQETCLQVDTIQRTIPELRENTRLVPTNNLHLTL
                     KFLGDTDEKVIPTISQALDSSITTIDRFEFIWEGLGCFPNNHKPRVLWLGVGQGLEQI
                     QKLTLAIESVLENFGYQPEKREFRPHLTIGRVKNPFKKVAGLESFLNYKINPTINPVK
                     DVIFFESNLTMGGPIYTRLAVFNLK"
     gene            complement(4945..6174)
                     /locus_tag="PLI55_02130"
     CDS             complement(4945..6174)
                     /locus_tag="PLI55_02130"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPA99668.1"
                     /translation="MTNAAIISIGNELISGLVNNTNSAYIARTLNNFGIVVSLIVTVG
                     DEQKHIWTVLDNLPPEINLVMITGGLGPTNDDITVSALARYFNLPLVFNKETASNLVK
                     HFAKRGLKMPEINLKQAYLPKEAVILDNPVGSAPGLKIEHQNRVYYVLPGVPREVEAI
                     MTKIIAPQLAALGHKAIVKKYLHITGIPESQIYTLLKEWIEQHSHVKVAFLPQTARID
                     LELTTYEPQESAKIDEYVKEIAEILGDYVFGFDDDTIESVIAHLLLPKHLTVAVAESC
                     TGGLIASRLTDVPGSSNYFKTGIVAYSNATKINLLGVKPEIIEKYGAVSSEVANQMAT
                     RVRQISGCDIGLSSTGIAGPSGGSDAKPVGLVWIGVSDKNATRTFQYQFSGDRSTNKL
                     RFSQAALNQLRLYLIGK"
     gene            complement(6171..6650)
                     /locus_tag="PLI55_02135"
     CDS             complement(6171..6650)
                     /locus_tag="PLI55_02135"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HPA99669.1"
                     /translation="MNITGAKKLLSRIIASVCFIGYLPFAPGTWGTVAGLVVWFLLPW
                     HSFWFDLILVTVTFFIGIFTSEYIEKQIGEMDPAYIVIDEVVGVWLALAIIPTLTYPK
                     DLALIVLVFLIFRIFDIKKFKPIRKLELIGGGFGIMADDILAGIFTGIIVNLGILIL"
     gene            complement(6647..7246)
                     /gene="pgsA"
                     /locus_tag="PLI55_02140"
     CDS             complement(6647..7246)
                     /gene="pgsA"
                     /locus_tag="PLI55_02140"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HPA99670.1"
                     /translation="MKKLFNWPNLLTFFRILLTPLFLYFLFGNQPYFEIFALLIFIIA
                     SVTDIYDGYIARKYASVTKLGKFLDPLADKILMSAAFISFVILDLVPLWMVILVILRD
                     FIVTGIRILMNSRHLTMETRKSAKTKTGVQVGVVCFILAYLITQRWEIFLGISDYVRL
                     IEEYRIIYILMLITTIITIWTGVEYILVNKDKIQQALKK"
     gene            complement(7248..7841)
                     /gene="recR"
                     /locus_tag="PLI55_02145"
     CDS             complement(7248..7841)
                     /gene="recR"
                     /locus_tag="PLI55_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPA99671.1"
                     /translation="MSALPDSLQNLIERFARFPGIGRKSAQRLAFYLLKADREEAVAL
                     AKAIIDVKDRIYKCSQCYNISETDPCKICTDEKRDHTTICVVQEPMDVFSIEKTGHYR
                     GLYHVLGGVLSPLNGIGPDDLNIQGLLYRLANVKEVILAINLTRDGETTALYLSKLLK
                     SKNVKVSRIAQGLPAGIELEYADEITLTHALEGRTSF"
     gene            complement(7838..8176)
                     /locus_tag="PLI55_02150"
     CDS             complement(7838..8176)
                     /locus_tag="PLI55_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011866032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPA99672.1"
                     /translation="MIPKGGLNNLMKQFSKVQAEMERVQEELANLTVEGSAGGGMVKV
                     VANGKQEIQSIKIDPEILKDDVEMVEDMIVAAVNQALARAQELSQEKMADLTGGMLSN
                     LPGGMKIPGL"
     gene            complement(8186..9838)
                     /gene="dnaX"
                     /locus_tag="PLI55_02155"
     CDS             complement(8186..9838)
                     /gene="dnaX"
                     /locus_tag="PLI55_02155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPA99673.1"
                     /translation="MNYKVISRKYRPQNFNEIIGQDHICTTLQNALKSGRIAHAYLFT
                     GSRGIGKTSTARILAKALNCLNPQDYNPCNVCQNCVEITNSRSLDVIEIDGASNRGID
                     QVRELRENVKYPPSNSKYRIFIIDEVHMLTREAFNALLKTLEEPPPHVVFIFATTEPL
                     KVPPTIISRCQRYDFHRIPVREIVGQLKKIAESENLTVSDDILTLLAKKSEGAMRDAE
                     SLLEQVVSFSSENVNLNSIQKLLGLTDYEYYFTISNQVFEHDLNALIKSAEEVFNNGV
                     NISEFLIGLSEHFRNLLITAIVKSAADLDLPDSLKNHYQEEAQRWSVNDLMRLLKLVG
                     EAQAGLKAAMNQRTHLEFALLRMGAMEKTVTIQSILQNIDARQSGGIGQIKPVAQTMD
                     FFSTKPVVPKTVPHPDSNVIKEKVSDNYIPEKKSEPISKSFDSLVTFEAIQAQWSTII
                     LKLEESNLSLAEFLKVSKPMQLNGNVLDLGFTSEQDFAHKNVAKRAPTIEQILIGFFH
                     KPIKIHCIQIESAAESADTVKKPIDEMTEKVVDMFDGEIIIT"
     gene            complement(9850..10116)
                     /gene="ffs"
                     /locus_tag="PLI55_02160"
     ncRNA           complement(9850..10116)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLI55_02160"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(10146..10218)
                     /locus_tag="PLI55_02165"
     tRNA            complement(10146..10218)
                     /locus_tag="PLI55_02165"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10183..10185),aa:Ala,seq:cgc)
     gene            complement(10439..10909)
                     /locus_tag="PLI55_02170"
     CDS             complement(10439..10909)
                     /locus_tag="PLI55_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99674.1"
                     /translation="MANYYTLNKNLSTRRTTSPTEETPPSPAVKIDFSDTEQRYEYIR
                     SKLDDIMENIGQCYSERLTKELLHRLEKTILDFHTEVIALLDKLEKLEIERNQLPERT
                     PVSESVSTPVESQSGERAKRIEESEQRKAGIVIESELNKKTESEPKRRFFNRRK"
     gene            complement(10888..11793)
                     /locus_tag="PLI55_02175"
     CDS             complement(10888..11793)
                     /locus_tag="PLI55_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99675.1"
                     /translation="MKNESAKPESPHLIWGLPGRGNLAFIISLLISLFINFRAGISQE
                     ILVIHPNVGATIDLEEKIYYDLFPEFYNFSDAQFYQTSSDTVIAKIRLWIDNGYQEQL
                     LYFTPYQMYLIASRISVREPLTDENRQLIQKRYKPLYAEKFLAEIPMNSYCRLKLKDK
                     SQFDAVYYRLKGDSLLFWIDRQVVPINRNDLVKLKYWDNYRPNNWVKWGTMGSVAVIS
                     YFGSGLVADWLNITTKNAILTQFAAASVGCVIGYKISPIVNEYIMPSTVIKFPTGNIK
                     RLDTIARMCYNIRNIKDKIWQIITP"
     gene            complement(11807..13972)
                     /locus_tag="PLI55_02180"
     CDS             complement(11807..13972)
                     /locus_tag="PLI55_02180"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPA99676.1"
                     /translation="MKKYIVSLAFLLLCNLAIAQNQMATVLKVAGDVKLRTKGRSDFS
                     RKVTMGMGINTGDALKTGSDGFVAIIFTEDKSLLKIRNDSEIELIKDNLGDRTSKVTQ
                     GKVLAEITPGIKTSFRIETPTSVASVKGTKFWTITIPDFGDKFYGVEGQVGILNLITG
                     LEADLEPGKMVVSTIDGELFSLPVDPDEIPVDPEEMKPEEEPGEPSGMKMEEETEMPA
                     MGAIEATGQPPAESVAEAGKKEEEAQKAKPFGMGLGLGSVTIGGKIYNQIALRPEFKF
                     GKLGVSLDIAFYMDENGNIRKEEWDEFIDYIDKIYYVRWAQQGDPFFIKVGALDNVSM
                     GYGILLNGYSNTTEYPQVRKVGIHTGMQWSKIGWEAYIANLKEITGPGLMAGRLTYRP
                     ISKFPLVLGTTVVADVYPYKGLPDADEDDVADALDLYPEADDNVVLDSLKTGFTLGQR
                     EFLRNNGFNIPSETIIQNGITKLSDYDRLLNGAVSVDVGVPILNKKSFNLTVYGQAAS
                     FFPVEDSAVVYNSATQSYDRVAFTPGYGFAVPGVKMGIARIANLTLEYRYAAENFLFN
                     YWDRAYDYERVQIRNNQIYTKQQMSLMQNSMQGIYGAIDVNIMNYFILGGYYQHMFSE
                     GTEIKSFMASASIPKGIVPKLADATAFYQRNNDENPFDFKNPSENTILGYRIGFELGG
                     GAIIYYKFQRTYRDFDGNGIVDPDLEAVNLTTIETGFSF"
     gene            complement(13996..15231)
                     /locus_tag="PLI55_02185"
     CDS             complement(13996..15231)
                     /locus_tag="PLI55_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPA99677.1"
                     /translation="MDTAILFLPERMASNKANSIFNTIRGILHTQLNLNIVIQNLQNS
                     PPIYEQITSIKNALVFASIYDSIYLEKFENFQPLFKIKTKSSDNILVIPQKATVRHLK
                     ELAGGKVLITKGIDHNCVNPVILYLIKNCGIFFAPKSLSIYYEPKTLLDQLLKGDVSA
                     ILTNTFQFDLWEADEQQSLKILGKCPEESEIVALIGPKFNVRKLKKNFNDWLQEHGSD
                     YFKDGYKLQSIDKYENILLIEAIEGLGYKLKDFIENYPDLIIKNIKSKLAEEVETLNE
                     KYSGLQVFNEKLVDMYKEIRESHDRLYQEIDSAVENQILFLKDGTIIGISRGFMNWVQ
                     KSRPEIIGKNINDFINPNINKPFNELIQQVDYGLIKSFGIRLKNTNDNDTTAKMDFTI
                     LELRDSKVVMGIVSMITDR"
     gene            complement(15242..15610)
                     /locus_tag="PLI55_02190"
     CDS             complement(15242..15610)
                     /locus_tag="PLI55_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA99678.1"
                     /translation="MNQYKILIVDDEQNVCEFLGEFLQDKGYLVIKARSGSKALRYLE
                     KNNPDLVLLDILMPGMSGLEVLKRIRKLYPDLPVIILTGVKDKRVVDDIINIGPVDFI
                     PKPIDLDVLEKCISSNLTKK"
     gene            complement(15758..17020)
                     /locus_tag="PLI55_02195"
     CDS             complement(15758..17020)
                     /locus_tag="PLI55_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99679.1"
                     /translation="MKNGWQIAYFLMLSFLLFGLVGAQTPNSHPINLFILTFTNYEGA
                     PEIDWWKEGFVDFLMDYFSNNPDLNVHRINDPATIPNQIKNLSETEITRCYLLTGNYK
                     IIDQKFEVDLELIDINTWRLLQKKNISIQSADMAKIIESVNHGATEVLTPYLIKKAET
                     TVAQEISSPSQPTKNNYQKAVAASVAISSVIDKFSETNLESAQHEPSFGGTVTNELSL
                     KFGEAIAQTGSFQYTINQIIRNPYKLEISDPLFRRLSANDEIINVTFFINYVLSRDLI
                     QDMLTTFPYEIQTKNDEYTEYIFSGKRFAYSEDFIKAIARGDYRTHPVYAFITNTETV
                     ACVLIDMPIMYRKNLISSSKVQFTAKYQPLINVTANGWNVVIQLKNTDARAEYNFDIS
                     FEQLKNLSRIDVMFLSEADIQEYLKIMN"
     gene            complement(17030..18469)
                     /gene="gltX"
                     /locus_tag="PLI55_02200"
     CDS             complement(17030..18469)
                     /gene="gltX"
                     /locus_tag="PLI55_02200"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPA99680.1"
                     /translation="MTENTRVRFAPSPTGSLHLGGARTAIYNWLFARSVGGKFLLRIE
                     DTDVERSRKELVNQICDSLQWLGLNWDEEPVFQSQRLELYQQKVTELLKAGKAYRCFC
                     SSEELEAEKAATIKQNKAYRYSGKCRSLSLQQSEELMAQNKPYTVRIKLEPGVIEWDD
                     LVYGHIAVNNNELDDFIIQRSNGVPVYQMAVVVDDIDMRITHIIRGEDHIPNTPKQIN
                     LFRAFGQPIPQFAHLPLLLGPDNKRLSKRHGATGVDEYREQGFPAEAVVNYLALLGWA
                     PQNDQEVLSIDEIIQQFQLTAISRKPAVFDIKKLEWISGEHLNRMPVAKIRSVLVPKL
                     IAAGLINQQESENRTQYVDSVIELLRGRVRTYNQYLEWGCYFFQDPENYDEKAAAKYW
                     HTPETNEHLAKLSQELATLTDFSANTIEETLRRTAEHAGLKAADLIHPLRLALTGFGI
                     SPGIFQVAEILGKETVLRRIQKAIEYFKT"
     gene            18776..19702
                     /locus_tag="PLI55_02205"
     CDS             18776..19702
                     /locus_tag="PLI55_02205"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPA99681.1"
                     /translation="MLLFSLASFVFADEVWLLINPETRYASLAGAATAIVNDVAALYY
                     NPAGLANGDELSASANYGTWLGNSEIYYAGAVRNFKHRGGLGTSLYLYNLDAKITGDD
                     YTATDFSYNFIYVSIGYGCNFTRKITLGAATKFIYTNAPNTASDGIQSINGKGLACDL
                     GIQINNLLPFLTIGENKTGGLSLGAAFCNLGPAIKYRNDFTNYKSKLVPRLRLGGGWR
                     LLNKDLISATISYDFIEDYYDSNSSNHLKSHLIGGAFSFYKRLYIHCGQHIENNENYI
                     TWGVAYNLDKFIIGYCRYPSQKSNFFSVSYSL"
     gene            complement(19799..20242)
                     /locus_tag="PLI55_02210"
     CDS             complement(19799..20242)
                     /locus_tag="PLI55_02210"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HPA99682.1"
                     /translation="MKSKFWDWLELFIRLVIGFLFIYAAVGKIIHPEKFAVIVYNYRV
                     LPVELVNITAILVPWLEIALGVTLILGIWLETAAFLLSILIIVFIGLIISAIIRGLNI
                     ECGCFTLSKTGSLVSWKRVAEDLLILAGSLFIFFRHLPQEQSTTE"
     gene            complement(20239..20781)
                     /locus_tag="PLI55_02215"
     CDS             complement(20239..20781)
                     /locus_tag="PLI55_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPA99683.1"
                     /translation="MVKTILLRSIWLGLGVIVIGLIFNWLSPNGIPLVAKYQTVTVRG
                     KSQKIPLFMNRKAQTNQETVFEISPVSELDTEKAWQFFTNGGTIFIDTREYEDYLAGH
                     IAGAISIPLSEFQNDPTIVEELDRQVRIVTYCDGSDCQSSIDMAVRLNEMGFEQVWFY
                     FGGWKEWVEADLPIVKGSKP"
     gene            20870..21781
                     /locus_tag="PLI55_02220"
     CDS             20870..21781
                     /locus_tag="PLI55_02220"
                     /inference="COORDINATES: protein motif:HMM:NF028121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HPA99684.1"
                     /translation="MSSKNSIPAIKFLSFTLILCLGGLSCSPQRFLFNKVSNVFDSGI
                     EIIYQEGDLELAERFLSSNLETIVILVAQDPENPRLNLMAAQAFGAYAMAFVEDKDPQ
                     RAGLLYVRGLEYAFKSLPPQIRFSRNITPADLEKLLVESRKSDVPQLFWVGYNWGMYI
                     LQNLDQPRLLGDLAKVELLMRRVAELDAGYNFGGVDIFYGTFYSARPPMLGGNPELGR
                     SYYLKNIERNPNFLMTKLYYARYYAVQVQDKELFDNLLTEIETFDLESAPEIRLFNAL
                     AKQKATNLRQNAELFLDLNEILETNER"
     gene            21789..22799
                     /gene="dctP"
                     /locus_tag="PLI55_02225"
     CDS             21789..22799
                     /gene="dctP"
                     /locus_tag="PLI55_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPA99685.1"
                     /translation="MRMKYINAVILIILCTIPNLVAQKVHTIKVATVAPEGSSWMRQF
                     RQFEKEVDALTNGQVKFRVYASGVQGGEKDILRKMQLGQLDVGTFTGVGLGEIVPEVR
                     VFDLPFLFRNSAEVDYVCDALFDEFQTKFLEKGFFLAGWAEVGFVYLFTNTPVTRLAD
                     FKNVKMWLWKGDPLAKATFDEMKIAAIPLDITDVHTGLQTGLIDGVYISPYGALAMQW
                     HTKTKYMLNYPLTNSIGAILITTKKMNTIPEDLRNILIKKTKENTRQIVLAGRQENLE
                     SIQILKENGMQLTNLKSDAAAEFIEAGVRTRDRLAGVLYSREMLDRVLALLDEYRAEH
                     AE"
     gene            22789..23265
                     /locus_tag="PLI55_02230"
     CDS             22789..23265
                     /locus_tag="PLI55_02230"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HPA99686.1"
                     /translation="MLNKIRTIIEKFVTALAVVLMLFLVVMAFLQVILRNFFAIGLNV
                     VDELMRNGVLWIAFCGAALTTLRSKHISIDILPRIVKGKARLVLNWILNLTAAAICLC
                     LSWYAWQFLRLEISMNSMIAGVIPAWIIEIILPVGFFLLALTFLLRLMDKSQNLEN"
     gene            23265..24536
                     /locus_tag="PLI55_02235"
     CDS             23265..24536
                     /locus_tag="PLI55_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015403675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPA99687.1"
                     /translation="MALLFLVLIVVLILCGLPLFAAIGITGIIQFAISGIDPTVVIVE
                     MYRVASAPTLLAIPLFTLAGYVLAECSAPRRLLNLAEALLGWIPGGVAIGSLVTCALF
                     TCFTGASGITIIALGGLILPILLKNNYSEKFALGLITSSGSLGLLFPPSLPLILYGVV
                     ASVSIDQLFIAGLIPGILLIILLSIYAVRNDVLKKSERIPFSWQNLGRALKETVWELP
                     IPVIILGGIYGGFITAVEASAVTAFYVLIAEFILNRDLSFRRDLIRIMRDSVQLVGGI
                     LIILSCSMGATAYLIDAEIPQKALLWAQQVFSSKITFLLVLNIFLLITGMMLDIFSAI
                     IIVVPLIVPIALSYGINPVHLGIIFLTNLEIGYLTPPIGLNLFISSYRFDKSITELYR
                     ISLPFLFIMLIALVLITYIPDLSLFLVGLIQ"
     gene            complement(24630..>24889)
                     /locus_tag="PLI55_02240"
     CDS             complement(24630..>24889)
                     /locus_tag="PLI55_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA99688.1"
                     /translation="ISAIIRYVGEQYIDEANIGMNPAYWLTDVGLRYSFGRVEIGAKV
                     NNLFNRLYSTYGYGYEWGEYYAYYWPGATRNAFFSISYHLD"
     assembly_gap    24890..24989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24990..26920)
                     /locus_tag="PLI55_02245"
     CDS             complement(<24990..26920)
                     /locus_tag="PLI55_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA99689.1"
                     /translation="MHQKNLPVFFFILFILILTEANAFGEEHLIFGKLVSETTGQPVE
                     SANILLEDGSGTFSDKDGNFRIKTSFLPIKVTVTHIAFRDTSFSATSEDCGTIYLKPT
                     VITGEYVFVTATRAVKGKTPVAFSELSIEEIKSRYTVEDVPMILAIEPGVYSYSESGN
                     GTGYSYVQIRGFDQSRIAVMLNNVPLNDNESHQVYWVDHGDILSDAADVQIQRGIGNS
                     LYGSSAFGGSINVLTTIGTDRPTFSIDLGGGSYNTSKIRLGASSGQLHNGKIRLSARL
                     SQIESDGYREYHETFQRSGSAGIEYNGQNTSHQFRALVGYQKSNLMWDGVPAELINDR
                     IGRRSGYKSYIDNFLQQVYSLNSTWRISENLNFNNVAYLVKGDGYYESEKSAAYNLAF
                     DDTTARDEFRDFLRSFNLDQYYPTDADSLSLELTRRKWIVNSYYGIVPTLTFIKAKYR
                     LDIGGEFKYYNGNHFGEISRFSDQNLNAQIGKGWYRYYRYIGEKSIATGFVHFTLEPL
                     KKLKLITDLQYQHLQWNLDQKKIGYAIGYDISAKWDFLNPRFGAIYELSNDFSIFANY
                     GRSQKEPADEQIIEADDYWSEPQKAAAELIDDYELGANYRGRKLVADLNLYRINYKNE
                     QLKNIDIEQEGEYVYTQASA"
     regulatory      complement(26910..27012)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(27075..27167)
                     /locus_tag="PLI55_02250"
     tRNA            complement(27075..27167)
                     /locus_tag="PLI55_02250"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27130..27132),aa:Sec,seq:tca)
     gene            complement(27172..28014)
                     /locus_tag="PLI55_02255"
     CDS             complement(27172..28014)
                     /locus_tag="PLI55_02255"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPA99690.1"
                     /translation="MKRFVFLILIPAFIFAQNIGELKQKIRAGEVDSVRTLLPALIAE
                     YPDNPELLYISGMIETDGEAALLIYRDVLSRFPSSSVADDALLKIIEYLFTKGLYTKT
                     EKYASELIKTYPQSDLIDKAIYLRLCSFNALNKRDSVDYYYKYYSTQYPNLNFHFEDY
                     KIASKVTSIESKKQSQPTSSQISSNAKSAETQTYSLPKVEVPGQGNFTLQMGVFGDPA
                     NARALRSKLEKLGYSVTLRQIERSGRKLTAVLAGSFKSEEEARAVGNKLKKDHNLDFI
                     VVKQ"
     gene            complement(28011..29159)
                     /locus_tag="PLI55_02260"
     CDS             complement(28011..29159)
                     /locus_tag="PLI55_02260"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99691.1"
                     /translation="MSLLDILLTLVLIALIGFIVINLTGRRRLSRSKDSASLYAMALN
                     HMLHGEDDVAIRLFRDVIRADTDNIDAYINLGILMRKNGKPKNAIKIHESLLYRQEIS
                     NIQRLEILRNLVEDYIAAGDKIRALNYVENILSIDKKNIWALEKNHILNRDLGRWEKA
                     SEYLAMALELKKERNDRLLALYKVQEGLVKYQAGEYHEARLIFRKALKIDPNCEAAYY
                     YIGKSYVLDHREEDAVEEWVKFTDVAPHKAYLIFKPLQTILFNLGNFGNIESFYENLL
                     RRIPDDPKTLIALADFYEKKGNAKKAIAILEDLVEKNPGWSIAKINLAKLLVGQAELN
                     RATAILNDIIEKMVDQKEYICSNCGNRSSDILWICPVCGLADTYFTDV"
     gene            complement(29172..29498)
                     /locus_tag="PLI55_02265"
     CDS             complement(29172..29498)
                     /locus_tag="PLI55_02265"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPA99692.1"
                     /translation="MKFLRWVVTLLIMFLIVFFVIQNSNLNPPVKIQFFWKASPVEMD
                     ILILVFLTFILGLVIGFLIAGIKILTTKNELRALSNDYKKLKKEVDLLRNQGIDETEA
                     DNSKTE"
     gene            complement(29513..30811)
                     /gene="miaB"
                     /locus_tag="PLI55_02270"
     CDS             complement(29513..30811)
                     /gene="miaB"
                     /locus_tag="PLI55_02270"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPA99693.1"
                     /translation="MEARKFYIETYGCQMNEADSELVIGLLRGANYQLTAIPEEADLI
                     LINSCSVRENAEKRAIARLAQFKSLKKKKPELLLGLIGCVAQRAHDEILTENKFVDLV
                     LGPDSYRNLPEYLNQEQMPIVDVNLSRTEVYDELIPSRNSAVNAWVSIMRGCNKFCTF
                     CIVPYVRGRERSRSPQSIIDEIRQALDDGYREVTLLGQNVNSYHYEAYRFPELLEVVA
                     AVPDLRRIRFTSPHPGDVDEKMLAVMQSHSNICKQIHLPLQAGSSRILKLMNRSYDQV
                     HYLKVVNLIREFLPQVALTTDIIVGFPGETADDFEQTLQVMQTIKFDSAFMFKYSPRP
                     GTKAAKMNDDVPEEEKSRRLKAVIQLQKSHTLERNKALVGTVQEVLIDGTSKKNPDEM
                     IGRTESNKIVVIKEGHPKIGEFCNVQIEKAMGVSLFGKII"
     gene            complement(30892..31812)
                     /locus_tag="PLI55_02275"
     CDS             complement(30892..31812)
                     /locus_tag="PLI55_02275"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPA99694.1"
                     /translation="MKKAPKLLFVVNTRKRVHSISLSWGKLVAVLAGGVFLLIFVIIL
                     GANLFSNLVYQSRLTRLKKNNNRLVSTFYDLNNRIQKMESDISMLVEKDKALRTYGDL
                     PVIDQDIRRLGVGGRLMPKSDELDQLLPESDIKVSELVNNLDRLAREIKFERISYETI
                     YDAFKNRSDQIQSTPSIRPVYTGFINDGFGYRRDPFTGRREFHYGLDITTPTGTPVFV
                     TADGVVGKSGYQGGYGYVVEVNHGYGYSTLYAHLSKILVRSGEAVKRGQKIGEVGTTG
                     RTTGPHLHYEVKQFNINKNPLDFFFSGYIR"
     gene            32215..>33369
                     /locus_tag="PLI55_02280"
     CDS             32215..>33369
                     /locus_tag="PLI55_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99695.1"
                     /translation="MKKGGYVLIMIAVLSTMAEVGWGQVSITQFETAYNQDFNSLANT
                     KTDNAWTDNSTISGWYSNRTEYIADNGSSTTGGLHSYGSADASERALGGLSSSSASPA
                     FGIRLVNNTGNTITSFNISYKGEQWRQTANSHTLVFSYQVDATGIESGTWTSFSDLDF
                     TALKTGTSSALDGNAEGNYQNVSSNVIIEVPNGHEIWFRWLKTGTNSPGLAIDDLSIT
                     AYEIKVPTISVNPSSLSGFSYVVGSGPSAEQTFTVSGTDLTADISISAPEHFEISKTS
                     GSDFTSSLTFAQSDGSVNETIIYVRLKAGLAIGSYSSENITCSSEGADDRTVTCSGTV
                     TSPPMHYRSKITGNWGDNSTWESSTDQTTWSNASLSPTSNDYTITIQNGHT"
BASE COUNT         9584 a         7233 c         6396 g        10056 t
ORIGIN      
        1 gtcgccagat gcgcctgacc gccgccaccg ccacctaaaa ttttccccaa ttcccgaaca
       61 atatctccag cctttagtcc atatcgtttg atggcatggt cggtgattac cacagtaata
      121 ttcggttttt catcaattat accggctatt acaccgacgc cgttatgcaa tttagcgcgc
      181 accagatcac cgatttcctt taattcagcc agacttttga cattcgcttg ataagtaaaa
      241 acctttaatt ccccaatcat ctcggctttt tctaagagag tatcaatctc atgttcaaga
      301 tccgctttat tgtttttatg aaattgtttt tctaggtttt tgtaatcagt taataatttt
      361 tcaattcgtt gaggtaactg ctcacggcta acgccaagga tagtcgtaat cttttccagc
      421 aggcgttcat tttctcgcat taattgcaac accgcctcgc cggtaatggc ttcaatccga
      481 cggacaccag cggcaatcgc cgtctcgcta ataattttga aacagcctat atcaccggtt
      541 cgattgacgt gtttaccgcc gcacagctct tttgaacaat caccaacgtt gattactcgc
      601 acgacatcgc catacttttc gccaaacaac gccatcgcac ccgattcacg agcactctga
      661 taatccttca cttcagcatt caccggcaaa ttttctcgga tgcgggcatt aacccaatct
      721 tcaactgcct ctatttctga cttggtcatt ttagtaaaat gggtaaaatc aaaacgcaat
      781 ttttccggtg tcaccagcga accggattga tggacgtgct cacccagaat ctgacgtaaa
      841 gccgcctgta aaagatgcgt ggcagtgtga ttggcgcgaa tctgcaaatt agcgacgttg
      901 tccacgctgg ctttgaccat cggattatca attaaattcc ctttttcaaa atgaccgcta
      961 tggataatgg aagagccaat tttccgcgtg ttgtcaatcc tgattctgaa attattgccc
     1021 tcgataattc cgacatcgcc gacttcgcct ccagcctcag cgtaaaacgg agtccggtcg
     1081 agaatcaaat gaactttctg accttcgatc aggtacttgc gaatcaccga ttgcgtttcc
     1141 agattttcat agccgacaaa ctcagaatcg tcgccagtcg tcagcgtttg ccaatcttca
     1201 ttttccagaa aatcatcggc ttgaaacact accgaagccc gagcacgttc tttctggcgg
     1261 ttcattaagt tttcaaatcc cttttcatca atgctaagtc cggcttcctc agccaatagg
     1321 cgagtcaaat ccattgggaa accaaatgta tcgtagagtt taaaaacttc ttcaccagga
     1381 attattgtga tattatttcg ttgcaggttt tccttgagtt tagcaaagac ctccaatcca
     1441 cggtcaagag tcaaaccaaa tgacttctcc tcggctttaa taactgcttg aatatgatta
     1501 cgccgttctg caatttcagg gtaagtcgca ccgagcacct caatcacact atcaactaac
     1561 tgataaataa aaggcttatg catttccagc attcgaccaa aacgagccgc ccggcgcaaa
     1621 atgcggcgaa taacataacc gcggccatca ttggacggca aaccgccatc cgcaatcgca
     1681 aacgtcagca ttcgaacgtg gtcggcaata acgcgatggg gcacaccgtt ttccgtatga
     1741 tattctttgc cggttaaaga tacaatccgg tcaatgatcg gcttaaataa atcggtatca
     1801 taatttgact ttttcccttg caaaacagct accatccgct ctaatccggc tccggtatca
     1861 acgtgcttaa gtggcagagg cttcaactct cccgatttat cacgatcaaa ctggataaaa
     1921 accagattcc agagttcgat atattccggg ttgccggaat taatcatttc cgcttgctgg
     1981 tgctccggat ggggaccggt gtaataatga atttcagaac aaggaccgca gggaccggtt
     2041 tcgcccatct cccaaaaatt gtccttttcg ccaaagcgaa gaatcctact ctggtcgagt
     2101 ccggctattc tctgccaggc ttgaagcgcc tcttcatcat cttgataaat agtcacccac
     2161 aatctttccg tcggcaattt ccagacttcc gtcaacaatt cccacgccat ttgcacggct
     2221 tccgctttat aataatcacc aaatgaccaa ttacccaaca tctcgaaaaa agtgtggtga
     2281 taagtgtctt tccccacttc ttccagatcg ttatgtttac cgctaacccg gatacatttc
     2341 tgtgaattgg cgattctcgg gctttcgatt ttctcctggc ctaaaaatat gcctttgaat
     2401 tggttcattc cggcgttagt aaatagcaaa gtcgggtcag attgcggaat aaccggggca
     2461 ctgcgaacaa ttttatgacc gtggctagcg aaaaattcta aaaactcacg acggacgtcg
     2521 ttggcggttt tcaattatac tcctgtaaac tttataaatg caaaaaatca tatttcaaat
     2581 ttactcttca ttaagcataa atatgaaggt gaaagttatc tggaatcggg cttataatca
     2641 agcagatatt ggcgaaggca aactttaagc actttatttc tatagaattt tttgcagtac
     2701 tttgataacc acatcgggct tatatccttt ttgaattagg taacgatata acttatcgcg
     2761 acgcttcgga tcgtttttga gagttgaggc ttttttttgt ccaatgatcc aagccgcctt
     2821 caattgatct tcatctttca ctaagttttt aatcgcctca tcaatcacct cacgatctat
     2881 ccctttcgca accagagcct gcctaatttt ccgaattccg ttatgattca attccagttg
     2941 atcacgaata aaggtttcgc aaaaaacttt atcatccaga tagcctcttt ccgtacaata
     3001 cttgattacc cggctagcca ccgtcaaagg atatttctgg cgacgggtaa gatataattc
     3061 gagttccctt tgactgcgta ttctaagtcc cagatatcta attgccgctt ctttcgcctg
     3121 ttcgaatagt tcgtgttgct gaatcatatt aatctgcttt tccgtcaatt gctgaccaac
     3181 gcaaatccga aaaaggttaa gagtttcagc actaatgccg aaagcaaatt cattatcgag
     3241 ataaatatta atgcgcgccg acttcttctt ttgcgattcg atcttggtta caacttttaa
     3301 tatctcggct tcggacgaaa agtctgagga cattttattt ctttctggat taaaaggaat
     3361 ttattccttg attagcccta gataagtttt aacctgctgt tcgagttttg taagcaattc
     3421 tttattttcg gtcaggtaac ctttagcgct ttcgcgtccc tgaccaatac gttcatctcc
     3481 atacgaatac caggctccac ttttttggac aaacccagcc tcgacggccg tatccagaat
     3541 atccccaaca tacgaaatgc cctgatcgaa agtcaagtca aattcagttt ctttaaatgg
     3601 tggcgccatt ttatttttaa ccaccttgac ccgcgtgcga cttccaacat tagtgttacc
     3661 ttctttgatc gtggaaattc ttcgaatttc aagacggaca gaggtataaa atttcaaagc
     3721 ccgaccaccg gtggtcgtct caggattacc atacgtcacc ccaatctttt cccgaatttg
     3781 gttaatgaaa atcaccgccg tgttggattt gctgactgca ccggtcaatt tacgaagagc
     3841 ttgagacatc aagcgcgctt gtaatccgac atgcgaatcg cccatttccc cttccaattc
     3901 ggcgcgcggc accagagcgg ctaccgaatc aatcacaatc acatcaatag cattactacg
     3961 aaccagagtt tcagtgattt ccagcgcctg ttcaccgtgg tctggttgcg aaatcagtaa
     4021 atcgcgggta tttactccca gtttagcggc atattggggg tcgagcgcat gctcggcatc
     4081 aataaaagcc gcataaccac cggctttctg agcctgagcg atgatatgca aagccagcgt
     4141 cgttttcccg gacgattccg gtccaaatat ctcggtgatt cgaccacgcg gaataccgcc
     4201 aaccccgata caagcatcca aagataagca accggtcgga attacatcca cggtgatttt
     4261 catcgagtca tctccgagac gcataatcga acccttgcca aactgacggt caatctgggt
     4321 tatggctaaa tcgagagctt ttattttatc atcatttttt tccgccatct ttctctcctt
     4381 attttatttt aaattaaaca cggccaggcg tgtgtagatt ggtcctccca ttgtcagatt
     4441 actctcaaag aaaattacat cctttaccgg atttatggta ggatttattt tataatttaa
     4501 aaaactttcc agtccggcaa cctttttaaa aggattttta acccgcccga tggttaaatg
     4561 tggtctaaat tcgcgttttt ccggttgata accgaaattt tctaaaaccg attcaattgc
     4621 aagagtcaat ttttgaattt gctctaatcc ctgaccgact cccagccata atacgcgagg
     4681 cttgtggtta ttaggaaaac acccgagccc ttcccaaata aattcaaatc tatcaatggt
     4741 agtgatgcta ctatctaatg cctgagaaat agtcgggata actttttcat cggtatcgcc
     4801 cagaaatttc aacgtcaaat gcaaattatt ggtaggtacc aggcgcgtat tttcacgtaa
     4861 ttccggaata gtcctttgga ttgtatccac ctgcaagcaa gtttcctgac taaccggtat
     4921 ggcaatgaaa gttcttttca tcttttattt acctattaaa tataaacgca gttgatttaa
     4981 cgccgcctga gaaaatcgta acttattagt cgagcgatcg ccactaaatt ggtattgaaa
     5041 agtccgagta gcgtttttgt cggaaacccc aatccagacc aatccaaccg gtttggcatc
     5101 actaccacca gagggaccgg ctatcccggt ggaactcaaa ccaatatcac acccggaaat
     5161 ctgtcgcact cgggttgcca tttgattggc aacttccgaa ctcaccgcgc cgtatttttc
     5221 gataatttcc ggtttaactc caagtaaatt aattttcgtg gcattgctat aggcaacgat
     5281 tccagtttta aaataattcg aactaccggg aacatcagtt aaacgactgg caatcagacc
     5341 gccggtacag gattcggcaa ccgcgaccgt cagatgcttt ggtaataaaa gatgagcgat
     5401 aaccgattcg atagtatcat catcaaaacc aaagacgtaa tcgccgagaa tttctgcaat
     5461 ctccttgaca tactcatcta ttttagcact ttcctgaggt tcataagtcg tcagctcgag
     5521 gtcaatacgc gccgtctgcg gcaggaaagc tactttgacg tgagaatgtt gttcaatcca
     5581 ttcttttaat aatgtataaa tctgagattc aggtattccg gtaatgtgca ggtacttttt
     5641 tacaatagct ttatggccga gagcggctaa ttgcggagcg attattttgg tcattatcgc
     5701 ttctacttct ctagggacac ccggcaaaac ataataaaca cgattttgat gttcaatttt
     5761 caagccggga gctgaaccaa ccggattatc gagaataaca gcttcctttg gtaggtaggc
     5821 ttgttttaga tttatttctg gcatctttag tccccgtttt gcaaaatgct tcactaaatt
     5881 actggctgtt tctttattaa atacaagtgg cagattaaaa tagcgagcaa gggcagaaac
     5941 tgttatatca tcattcgtgg gacctaaacc gccggtgatc attaccaaat tgatttccgg
     6001 aggtaaatta tcgagaactg tccagatatg tttttgctca tcgcctacgg ttacaattaa
     6061 gctaaccaca ataccaaaat tatttaatgt ccgagcaata taggcagagt tggtgttatt
     6121 aacgagtccg gagattagtt cattcccgat cgaaatgata gccgcattgg tcacaggatc
     6181 agaattccca aattgacgat aataccggta aaaataccag ccaggatatc gtctgccatt
     6241 ataccaaagc caccaccgat caactccaat ttgcgaattg gtttgaattt tttaatatca
     6301 aatattcgaa aaataagaaa cactaaaacg atcaatgcca aatctttggg ataagttaaa
     6361 gtcggaataa ttgccaacgc cagccagact ccaacgactt catcaattac tatatacgct
     6421 gggtccatct cgccaatttg tttttcgata tactcagaag taaaaatgcc aataaaaaaa
     6481 gtgactgtca ctagaattaa gtcgaaccag aatgagtgcc atggcaataa gaaccagaca
     6541 actaagcctg ctactgtacc ccaagtgccc ggtgcaaatg gaagatagcc gatgaagcaa
     6601 acgctcgcta ttatccggga gagtagtttt ttagctccgg taatattcat tttttcagag
     6661 cttgctgaat tttatcttta tttactaaaa tatattccac cccagtccag attgtaataa
     6721 ttgtggttat cagcatcaag atataaataa ttctatattc ttcaattaac ctgacatagt
     6781 cggagatacc cagaaatatt tcccagcgct gagtaatcaa ataagctaga atgaaacaga
     6841 caacgccgac ttgcactccg gttttcgttt tcgctgattt gcgagtttcc attgtcaggt
     6901 ggcgcgaatt cattaatatt ctaataccgg ttacgataaa atcacgtaga atgaccagaa
     6961 taaccatcca gagaggaacc agatctaaaa taacaaatga aataaaggcg gcagacatca
     7021 gaattttatc cgctaaagga tcaagaaatt tgccgagctt ggtcacgctc gcatatttcc
     7081 gagcaatata gccatcgtag atgtcggtta ccgatgctat aataaaaatt aacaaggcga
     7141 atatttcaaa ataaggttgg ttaccgaaca gaaaatagag aaagagaggc gttaggagaa
     7201 tccggaaaaa agtcaataga ttaggccagt taaatagttt tttcatatta gaaacttgtc
     7261 ctaccctcaa gagcgtgcgt tagggtaatt tcgtcggcat actccagttc tatgccggcc
     7321 ggaagtccct gagcaatgcg agaaactttt acatttttac ttttaagtaa ttttgataga
     7381 tagagcgccg tcgtctcacc gtcgcgggta aggttaatgg caagaattac ttccttcaca
     7441 ttggcaagtc ggtataacaa accttgaata ttcaggtcat caggcccaat cccgttcaac
     7501 ggcgacaata ctcctcctaa aacatgatag aggccgcgat aatgaccggt cttttcgatc
     7561 gagaacacat ccatcggctc ttgtacaaca cagatcgtcg tgtggtcgcg tttttcatca
     7621 gtgcaaattt tacaaggatc ggtttcggaa atattataac actgcgaaca tttataaatg
     7681 cgatctttga catctataat ggctttggct aatgcaacag cttcctcccg atcggctttc
     7741 agcaaataga aagccagacg ctgggccgac ttgcggccta ttccgggaaa tcgggcaaaa
     7801 cgttcaatca aattttgtaa cgaatcggga agagcactca taaaccgggt attttcattc
     7861 ctcctggtag attggataac attccgccgg ttaggtctgc cattttttct tgcgataatt
     7921 cctgggcacg agccaaagcc tggttgacag ccgcaacgat catatcttca accatttcta
     7981 catcgtcttt taatatttcc ggatcaattt taatagattg aatttcctgc ttaccattag
     8041 ctacgacctt aaccattccg cctccggcac tgccttcgac agttagattg gcaagttctt
     8101 cctgcactcg ttccatttcg gcttgaactt ttgaaaattg tttcatcaaa ttatttagtc
     8161 cacctttagg tatcatcaat actccttatg taataattat ttcgccatca aacatatcta
     8221 cgactttttc ggtcatttca tcgatcggct tttttacggt atcagccgat tccgcagctg
     8281 attctatttg gatacaatga attttgatcg gtttatggaa aaaaccaatc aaaatctgtt
     8341 cgatagtcgg tgctcttttg gcaacatttt tatgagcaaa atcttgttca ctggtaaaac
     8401 ctagatccag aacatttcca tttaattgca tcggctttga aactttgaga aattcagcca
     8461 acgaaagatt gctttcttcc aatttcaaaa taattgtcga ccattgtgcc tgaatcgctt
     8521 caaacgtgac taaactatca aacgactttg aaattggttc agattttttt tcaggtatat
     8581 aattatcaga tactttctct ttaataacgt tactatctgg atgagggact gttttgggaa
     8641 ccaccggctt ggttgaaaaa aaatccatcg tttgggcgac tggctttatc tgtcctattc
     8701 ctccagattg acgagcatca atattctgca ggattgattg aatcgtgacg gtcttttcca
     8761 ttgctcccat tcggagaagg gcaaattcga gatgagttcg ttgattcata gccgctttca
     8821 gaccagcctg cgcttcacca actaatttta ataaacgcat cagatcgtta accgaccacc
     8881 gttgtgcctc ttcttgataa tgatttttca atgaatccgg gagatccaga tcggcggcgg
     8941 atttaacaat cgctgtaatc aacaaattcc gaaaatgttc ggataagcca atcagaaact
     9001 cactgatatt aacgccatta ttgaatactt cttcagccga tttaatcaaa gcattaagat
     9061 cgtgctcgaa aacctggtta ctgattgtga aataatattc gtaatcagtc agaccaagca
     9121 atttttggat agaattcaaa ttcacattct cactagagaa tgaaacaacc tgctcgagga
     9181 gactttcggc atcgcgcatc gctccttcgg actttttggc taacaaagtc aagatgtcat
     9241 ccgaaaccgt taaattttcg gattccgcaa ttttctttaa ttgccccaca atttcacgaa
     9301 ctggaatgcg atgaaaatca tacctttgac agcgggaaat aatcgtaggc ggtactttca
     9361 atggttccgt agtagcaaag ataaaaacaa catgcggcgg tggttcctcg agtgttttca
     9421 aaagtgcgtt aaatgcttcg cgcgtgagca tatggacttc atcaatgatg aaaattcggt
     9481 atttcgaatt ggatggcgga tacttgacat tttcccgcag ttcgcgtacc tggtcaattc
     9541 cccgattgga agcgccgtca atttcaatca cgtccagact gcgactattg gttatttcaa
     9601 cacaattctg gcagacatta cagggattat agtcttgcgg attaaggcaa ttgagtgcct
     9661 tggctagaat tcgggcagtc gaagttttac caattccgcg cgaaccggta aagagatagg
     9721 catgagcaat gcggccactt ttgagggcat tttgcagagt cgtacaaatg tggtcttggc
     9781 caatgatttc attgaaattt tgagggcgat atttacgaga aattacttta taattcatat
     9841 ttacttaggc tatgcaccag gcattgaatc cgtccaggga tattcggtta aagtcgtcgg
     9901 ctggctttcg ctgattttgc ggcacataga aagggttatc taccgctgct tccttccgga
     9961 cctgacgggg tttgataatt ttccattgcg caaaactgaa agcgtcatcg cttccgattt
    10021 aacctattat ccccgaccaa atcctctgcc tggcattcca ccctgctaaa gcggatttca
    10081 ggttacaggg caccgctagc tccccgtgta gcatagccaa tcattttgtt cgtatcaaat
    10141 aacagtggag ctgatgggga tcgaacccac gacctcagag atgcgaaccc tgcgctctcc
    10201 cagctgagct acagccccgt gttcaacgta tgataatttt tagtggtcac tttttcaggt
    10261 acgattgaat ctcttccccg ctctagttga aatcagttag atagactata attccgttat
    10321 taccccgcta ttcagaaact atgtcctgac caccgttaaa aatcgtattc tattatcaaa
    10381 taacagatat ttttaaatta ctaaaaatca gttaatctat caaaagataa ttgagtactt
    10441 attttctacg attaaaaaac ctcctcttcg gttcactttc ggttttttta ttcaattctg
    10501 attctatgac tataccagct tttctttgtt cactttcctc tatccgtttt gcgcgttcac
    10561 ctgattggga ttcaaccggg gtgctaacgc tttcagaaac aggagtcctt tctggcaatt
    10621 gattgcgttc tatttccagc ttttcaagct tatctaaaag cgcaataacc tccgtatgga
    10681 aatcgagaat tgttttttcc aaacggtgaa gcaattcctt ggtcaggcgt tcgctataac
    10741 actgaccaat attttccata atgtcatcta atttactccg aatatattcg tagcgttgtt
    10801 cggtgtcgct aaagtcaatt ttgactgcgg gcgacggagg cgtttcttca gttggagaag
    10861 tcgttcgccg cgtggaaaga tttttgttta aggtgtaata atttgccata ttttatcctt
    10921 aatatttcta atattgtaac acattcttgc gatagtgtca agtcttttta tattgcccgt
    10981 cggaaatttt atcacagtgg acggcataat atattcattg actattggag aaattttata
    11041 tccgataaca caaccgacgg aggcggccgc gaactgagtt aaaatcgcat tttttgtggt
    11101 aatattaagc caatcggcta ccagcccact accaaaatat gatataaccg cgacacttcc
    11161 catagtaccc cacttgaccc agttattcgg acgataatta tcccagtatt ttaatttgac
    11221 caaatcgttc ctatttattg gaacaacctg gcgatcaatc caaaataaca agctatcacc
    11281 cttgagacga taataaaccg catcaaattg acttttatct ttcaatttta gacgacagta
    11341 ggaattcatc ggtatctcag ctaaaaattt ttcggcgtat aaaggcttgt atctcttttg
    11401 aatcaattga cggttctcgt cggtgagcgg ctcccggaca gagatacgtg aagcgattag
    11461 gtacatctga taaggagtaa aatataacaa ctgctcttga tatccgttgt caatccataa
    11521 cctaatttta gctattacgg tatcggaact agtttgatag aactgggcat cagaaaagtt
    11581 ataaaactcc ggaaagaggt cataataaat tttctcttct aaatcaatcg tagcgcctac
    11641 attcggatga attacgagaa tttcctggct tatcccggct ctgaaattga taaagagtga
    11701 aattaacaag cttatgataa acgctaaatt accccgtccg ggaagccccc aaattaaatg
    11761 agggctttcc ggtttggcac tctcattttt caatttataa aacgcgttaa aaactaaacc
    11821 cagtttcgat ggtagttaga ttaactgctt ccagatcggg gtcaactatt ccattaccgt
    11881 caaaatcacg gtaagttctc tggaatttat agtagataat cgctccgcca cccaattcaa
    11941 aacctatcct gtaaccgagt atcgtatttt cagaaggatt tttaaaatca aaaggatttt
    12001 catcattatt acgttgataa aaagctgtcg catcagccag ttttggaact attcccttcg
    12061 gtatagatgc cgacgccata aaactcttga tctcggtccc ttccgagaac atgtgttgat
    12121 aataaccacc aagaatgaaa taattcatga tattgacgtc aatagctccg taaattccct
    12181 gcatcgaatt ctgcatcagc gacatttgtt gtttggtgta tatttgatta ttacgaattt
    12241 gtactcgctc ataatcataa gctcggtccc agtaattgaa taggaaattt tcagcggcat
    12301 aacggtattc cagagtcagg tttgcgattc tggcgattcc cattttcaca cctgggactg
    12361 caaatccata tcccggagta aaagctacac ggtcatagct ctgagttgca ctgttataaa
    12421 ctacggctga atcctcaacg gggaaaaagg aggcggcttg gccataaacc gtcaaattga
    12481 aggatttctt attcagaata ggcactccta catcaaccga aaccgctcca tttaaaagcc
    12541 gatcataatc ggagagctta gttatcccat tctgtatgat ggtttcactt ggaatattaa
    12601 agccgttatt tctaagaaat tcgcgttgac ctaaagtaaa acctgtttta agtgaatcga
    12661 ggacaacatt atcatcagcc tccggatata aatccagcgc gtcggcgaca tcatcttcat
    12721 ccgcatccgg taaacctttg taaggataaa catcagcaac aacagtagtt cccaaaacca
    12781 atggaaactt gctaatcggt ctataagtca acctcccagc cattaatccc ggtccggtaa
    12841 tctcttttaa attagctatg taggcttccc agccaatttt gctccattgc attccggtat
    12901 ggataccgac tttgcggact tgcggatatt cggttgtatt ggaatagccg tttaatagga
    12961 tcccataccc catactaaca ttatccaacg ccccaacttt gatgaagaac ggatccccct
    13021 gctgagccca acgcacataa tatattttgt caatataatc gataaattca tcccattctt
    13081 ctttgcggat gttgccattt tcgtccatat aaaaggcaat atcaagggaa acgcctaatt
    13141 tcccaaattt aaattcggga cgcagggcaa tctgattata aattttcccg ccaatcgtaa
    13201 ctgagccaag tcccaatccc atcccaaagg gttttgcttt ttgggcttcc tcctcttttt
    13261 tacctgcttc ggctactgat tcggccggtg gttgaccagt cgcctcgatg gcacccattg
    13321 caggcatttc agtttcttct tccatcttca ttcccgatgg ttcacccggc tcttcttcgg
    13381 gtttcatctc ctcaggatca acaggaatct catctggatc taccggtaaa ctgaatagtt
    13441 ctccatcaat agtcgatact accatttttc ccggttccaa gtcagcctcc aaacccgtaa
    13501 ttaaatttaa aatacctact tgcccttcga cgccataaaa tttatctccg aaatcgggaa
    13561 tggtaattgt ccagaatttg gttcccttaa ccgacgccac cgaagtcgga gtctctatcc
    13621 ggaaggatgt cttaatgccc ggagtaattt cagccagaac tttaccttga gtaacttttg
    13681 aggttcgatc gcctaaatta tccttaatga gttcaatttc ggaatcattg cgaattttaa
    13741 gcagactttt atcctcggtg aagataatag ctacaaatcc gtcggaacca gtctttagag
    13801 catctccggt gttaataccc atacccatgg tcacctttct cgaaaaatca ctacgtcctt
    13861 tagtccgtaa tttgacgtct ccggctactt tcagaacagt cgccatctga ttttgagcga
    13921 tcgcaagatt gcacaacagt aaaaatgcta acgaaacgat atacttcttc attttttcta
    13981 ctcccattta aatttttatc tatctgttat catacttaca attcccatta cgactttaga
    14041 gtcccgcaat tcaagtatgg taaaatccat tttagctgta gtgtcattgt cgtttgtatt
    14101 ttttaaacgt attccaaatg atttgatcaa accataatca acctgctgaa tcaattcatt
    14161 aaaaggctta tttatatttg gattaataaa atcgttaata tttttaccga tgatctccgg
    14221 tcgggatttt tgtacccaat tcataaagcc tcgcgagatg ccgattattg tgccatcttt
    14281 gagaaataaa atctgatttt cgacggcaga atctatttct tgataaagac gatcatggct
    14341 ctcgcgaatc tctttataca tatcaactaa tttttcatta aaaacttgta acccgctata
    14401 tttttcattc agagtttcaa cctcttccgc taatttactt ttaatatttt tgataatcaa
    14461 atcagggtaa ttttcaatga aatcttttaa tttgtatcct aatccttcga tagcctcaat
    14521 tagcagtata ttttcatatt tatcaattga ttgtaattta taaccatctt tgaaataatc
    14581 tgaaccgtgt tcctgcaacc aatcattaaa attcttcttt aactttctta cattaaattt
    14641 aggcccgatt aaggcgacaa tttcagattc ttccggacat ttaccgagga tttttaaact
    14701 ttgttgttca tcggcttccc ataaatcaaa ttgaaaagta ttggtaagaa tcgccgaaac
    14761 gtcacctttt agcaattgat ctaataatgt ttttggctca tagtatattg ataatgattt
    14821 tggagcaaaa aatattccgc aatttttaat caagtataga atcaccggat tgacgcagtt
    14881 atgatctatt cccttagtaa tcagcacctt accacctgcc agttctttaa gatggcgaac
    14941 agtcgctttc tggggaataa ccagaatatt atcagatgat tttgttttaa ttttaaagag
    15001 cggctgaaaa ttttcaaatt tctctaaata aatagaatca tagatagaag caaatacgag
    15061 ggcatttttg atgctggtta tttgttcata aataggcggg ctgttttgga ggttttgaat
    15121 aacgatattc aagttcagtt gagtatgtaa tatgcctcgg atagtattaa agatactgtt
    15181 ggctttgtta gatgccattc gttcaggtaa aaataaaatg gcagtatcca ttgatattac
    15241 cttatttttt tgtgagatta cttgaaatac atttctcaag aacatccaga tcaattggtt
    15301 tgggaataaa atcaacgggt cctatattga tgatatcgtc aactacccgt ttatccttca
    15361 ccccagttaa gataataact ggtaaatcgg gatataattt tcgaatacgt ttaagtactt
    15421 caaggccgct cattccgggc atcagaatat ctaataaaac cagatccgga ttattttttt
    15481 ccaggtaacg gagcgccttg ctacctgatc tggctttgat aactaaatag cctttatcct
    15541 gcaaaaactc cccgagaaat tcacatacat tttgttcatc atccacaatt aatattttat
    15601 actgattcat tatttcccct ctaaatttaa aatctttaat gatagctaaa catttttcct
    15661 cttaattctg agacacggat taatttgtgt gatcagtttc atatatttat ttcttaatta
    15721 taatatattc aagaacaaat agataatcaa ataaatctta attcattatt tttaaatatt
    15781 cttgaatgtc ggcttctgaa agaaacatca catctatacg gctgaggttt ttcaattgtt
    15841 cgaagcttat atcaaagttg tattcggctc tcgcatcggt atttttaagt tggatgacga
    15901 cattccaacc gttagccgtg acattgatta aaggctgata tttggctgta aattgtactt
    15961 ttgaagaaga aataagattt ttgcggtaca ttattggcat atcaatcaga acgcaagcta
    16021 cggtttcggt gttagttata aatgcatata ccgggtgagt ccgataatcg ccacgcgcaa
    16081 tcgccttaat aaaatcttcg ctataggcaa accgcttacc actgaaaata tattcggtat
    16141 attcatcatt ttttgtctga atttcataag gaaaagttgt caacatatct tgaattagat
    16201 cacgactaag aacataatta ataaaaaaag taacgttaat tatctcatcg tttgcagaaa
    16261 ggcgccggaa aagtggatcg ctgatttcaa gtttataggg attgcggatg atctgattga
    16321 tagtatattg aaaactacca gtttgggcaa tcgcctctcc gaatttgagc gaaagttcat
    16381 tagtcacagt gccaccaaaa cttggttcgt gttgggctga ttccaggtta gtttccgaaa
    16441 acttatcaat caccgatgag attgctacac ttgcggcaac agccttctgg taattatttt
    16501 tcgtcggttg agatggtgag ctaatttcct gagcgacggt ggtctcggct ttttttatta
    16561 aatatggagt aagtacttca gtcgcgccgt gattgacaga ttcgataatc tttgccatat
    16621 ctgctgattg aatcgagatg ttctttttct ggagaagtcg ccaggtatta atgtcaatta
    16681 attccaaatc aacctcgaat ttttgatcaa ttattttata attgccggtt aaaaggtagc
    16741 atctggtaat ttccgtttct gagagattct taatttggtt tggtatggtt gccgggtcat
    16801 tgatgcgatg gacattaaga tctggattat tactgaaata gtccatcaag aaatcaacaa
    16861 agccttcttt ccaccaatca atttctggcg cgccctcata attagtgaag gtgagaatga
    16921 aaagattaat cggatgagaa ttgggcgtct gcgcaccgac caatccaaat aataaaaatg
    16981 ataacattag aaaatatgcg atttgccaac catttttcat tttaattcct caggttttga
    17041 aatattcaat ggctttttgt attcggcgca gaacggtttc tttccccaga atctctgcta
    17101 cttgaaaaat accgggacta ataccgaaac cggttaaagc cagacgcaga ggatgaatca
    17161 aatcagcggc ttttaatccg gcatgttctg cggttcgtct caatgtttct tcgatagtat
    17221 tagccgaaaa atctgtcagc gttgccaact cttgacttaa tttagccaga tgttcattgg
    17281 tctccggtgt gtgccagtat ttagcggcag ctttttcatc ataattttct gggtcttgga
    17341 aaaaatagca gccccattcg aggtactgat tataagttct gacgcgaccg cgtaataact
    17401 cgataacgga atcaacgtac tgagtccggt tttcagattc ttgctgattg ataagcccgg
    17461 ccgcaattaa tttgggaact aaaaccgatc taattttcgc gacgggcata cggttaagat
    17521 gttcaccgct aatccattcc aatttcttta tatcaaaaac agccggtttg cgggaaattg
    17581 cggtcaattg aaattgttgg atgatttcgt caatactcag cacttcctga tcattctgcg
    17641 gtgcccagcc cagtaaagct aaataattca cgacggcttc ggcgggaaat ccctgttcgc
    17701 gatattcgtc cacgccggtt gcgccatgcc ttttactgag gcgcttatta tctggaccaa
    17761 gcagaagcgg caagtgagca aattgcggaa tcggctgacc aaacgcacga aacagattaa
    17821 tctgtttcgg cgtattcgga atatggtctt caccccgaat tatatgagtt attcgcatat
    17881 caatgtcatc aacgacaacc gccatctgat agactggaac tccattcgaa cgttgaatga
    17941 taaaatcatc gagttcatta ttattcaccg caatatgtcc gtaaaccaga tcatcccatt
    18001 caatgactcc cggttcgagt ttgatacgga cagtgtaagg tttgttctgt gccatcagtt
    18061 cttctgactg ttggagcgat agactacggc atttaccact ataacgataa gctttattct
    18121 gtttaattgt agcggctttt tccgcttcca gttcctcact actgcagaaa cagcgataag
    18181 ccttgccggc tttcaacaat tcagtaactt tctgttggta aagttcgagt ctctgcgact
    18241 ggaatactgg ttcttcatcc caattcaatc ccagccattg taacgaatcg caaatttgat
    18301 tcaccaattc tttgcgtgaa cgctcgacgt cggtatcttc aatgcgcaag agaaatttgc
    18361 caccgaccga gcgagcaaat agccagttgt aaatagctgt tcgcgcacct cccagatgta
    18421 atgaaccagt tggactagga gcaaaacgaa cgcgggtatt ttcagtcata tattttctaa
    18481 catcatttat tttcaatcaa acttatttta aattcagcat aaagttctct aaaaaattat
    18541 acttaatcaa gagttttggc tcattgatgt atttcagagc ccattttttc aaggaaaagt
    18601 taatggttag ggttgacgga gtacagttaa taataacttt ctcaatgaaa attaataaaa
    18661 tacaagaatt ttaaaatatt gatttttgac cagcatttga ataaaataca caaccaaatg
    18721 gatatttaga attttcatgc aaaatatttc aaaatataga atccttcaaa atattttgct
    18781 cttattttct ctcgcatctt ttgttttcgc tgatgaagtc tggctattaa tcaatcccga
    18841 gacgcgttac gcatcgttag ccggagccgc cacggcgatc gtcaacgatg tcgcggcact
    18901 ttactataat ccagctggtt tagccaatgg cgatgaatta agtgcctcag ccaattatgg
    18961 tacctggctc ggcaacagtg aaatatatta tgccggagct gttagaaatt ttaaacaccg
    19021 tggcggtttg ggaaccagtt tatacctgta caatctcgat gcgaaaataa ccggagacga
    19081 ttacactgca accgattttt cctataactt tatctatgtt tccatcggat atggatgcaa
    19141 ttttaccaga aaaattactc taggagcggc aacaaaattc atatatacta acgccccgaa
    19201 cactgcgagc gatggcattc aatctattaa cggcaagggt ttagcctgcg acctgggtat
    19261 tcagatcaac aatctcctgc cattcctaac tattggagaa aataaaacag gtggattatc
    19321 cctgggcgcc gcattttgca atctcggacc ggctattaaa taccgcaatg attttaccaa
    19381 ttataagtcg aaactcgtgc ctcgcttacg actcggcggt ggttggcgac tgcttaataa
    19441 ggacttgatc agcgctacga tttcctacga tttcattgaa gattattatg attctaactc
    19501 ttcaaatcat ttaaaaagtc acttaatcgg cggcgctttc agcttctaca agcgacttta
    19561 catccactgc ggtcaacata ttgaaaacaa tgaaaattac ataacctggg gcgtggcata
    19621 caacctagat aaatttatca ttggctactg ccgttatcca tcgcaaaaaa gtaacttctt
    19681 cagcgtatct tattccttat aaaataagtt gttaaaatta ttggcaggat tgaactaaat
    19741 caatctggat taaaaacatt ttaccaaaaa gaaataaact ataatccgac cggtaagact
    19801 attcggtcgt agattgttct tgcggtagat gccggaagaa aatgaacaga ctgcccgcca
    19861 aaatcaggag gtcttccgcc actcgcttcc agctgaccag cgaaccggtt ttcgatagag
    19921 taaagcaacc gcattcgata ttcagacccc gtataatagc gctgataatc aagccaataa
    19981 agacaatgat caaaatactt aggagaaaag ccgctgtttc cagccagatt cccaatatta
    20041 aggttacacc aagcgcaatc tccagccatg gtaccaggat agccgtgatg ttaaccaatt
    20101 caacaggtag aacgcgatag ttatacacaa tcacggcaaa cttttcgggg tggatgatct
    20161 tgccgacagc ggcataaatg aacaagaaac caataaccaa tcggataaat aattccaacc
    20221 aatcccagaa tttggacttc atggcttgct cccttttaca atcggtaaat cagcctctac
    20281 ccactctttc cagccaccga aataaaacca aacctgctcg aaacccattt catttaagcg
    20341 tactgccata tcaatacttg actgacaatc actgccgtca caataggtca cgatccgcac
    20401 ctgccggtct aattcctcaa caatggttgg atcgttttga aattccgaca atggaatcga
    20461 tattgctccg gcaatatgcc cggcgagata gtcctcatat tctcgagtat cgataaagat
    20521 ggtaccacca ttagtaaaaa attgccaggc tttttcggta tcaagctcgg aaaccgggct
    20581 gatttcgaaa acagtttcct gattggtctg agccttgcga ttcataaaaa gcggaatttt
    20641 ttgactctta cctcggactg taaccgtctg atatttagcc acaagcggaa taccattcgg
    20701 tgataaccaa ttgaaaatta atccgattac aataacaccc agaccgagcc agatactacg
    20761 taaaagaata gttttaacca tttgtttcat cgtgatactt aataccatat ttaaaatatt
    20821 ctaaaaaatt tgtttttcaa aacaaagaat ataaattttt accgttataa tgtcgagtaa
    20881 aaattcaata ccagctatca aatttctaag ttttaccctg attttatgtc taggcgggct
    20941 ttcatgcagt cctcagcgct ttctattcaa taaagtttcc aacgtcttcg acagcggtat
    21001 cgaaataata tatcaagaag gcgatttaga actcgctgaa agattccttt cgagtaattt
    21061 ggagacaatc gtcattttag ttgcgcagga tccagagaat ccgcgtttaa acctgatggc
    21121 ggctcaggct tttggagcct atgcaatggc ttttgtcgaa gacaaagacc ctcagcgcgc
    21181 cggtctgctt tatgttcgcg gcttagaata tgctttcaaa tcactaccac cgcaaataag
    21241 attctcgcgc aatatcacgc ctgccgacct ggaaaagctc ctggtagaaa gccgtaaaag
    21301 cgacgtgccg caattgttct gggtcggata taattgggga atgtatattc tgcaaaatct
    21361 cgatcagccg cgcttattgg gggacttagc taaggttgaa ctcttgatgc gtcgagtagc
    21421 cgagttggac gctggctata atttcggcgg tgtggatata ttttacggaa cattttattc
    21481 cgctcgccca ccaatgctgg gcggcaatcc ggaattgggg cgcagttatt atctgaaaaa
    21541 tatcgaacgc aatccgaatt ttttaatgac gaaattgtat tacgctcgct attacgctgt
    21601 acaggttcag gataaagaac tatttgataa ccttttaacc gagatcgaga ctttcgacct
    21661 cgagagcgct cctgaaattc gactctttaa tgcgcttgcc aaacaaaaag ccaccaatct
    21721 gcgccagaat gccgagttgt ttctcgacct aaatgaaata ttagaaacca acgaaaggta
    21781 gattaaaaat gcgaatgaaa tatatcaatg cggtgattct tataatttta tgtacaattc
    21841 ctaacctcgt ggctcaaaaa gtccatacga taaaagttgc gaccgtcgct ccagaaggtt
    21901 ctagctggat gcgacaattc cgacagtttg agaaggaagt ggatgctcta actaacggtc
    21961 aggtcaaatt tcgggtttac gctagtggcg tacagggcgg ggaaaaagac attttacgta
    22021 aaatgcaatt agggcaattg gatgtcggga ctttcaccgg agtcggtttg ggtgaaattg
    22081 taccagaagt gcgcgttttc gatttgccgt ttctttttcg gaatagcgcc gaggttgatt
    22141 acgtttgtga cgctcttttc gacgaatttc aaactaaatt tctcgagaag ggattttttt
    22201 tggcaggttg ggctgaggta ggttttgttt atcttttcac caacacgcca gtcaccagat
    22261 tagcggattt taaaaatgtc aaaatgtggc tctggaaagg cgatccgctt gccaaagcca
    22321 cttttgacga aatgaaaata gcggcgattc cactcgatat caccgatgtt catactggct
    22381 tacaaaccgg gttaatcgac ggagtttata tatctcctta cggcgcgttg gcaatgcaat
    22441 ggcataccaa aactaaatat atgctgaatt atcccctaac taattctatt ggcgcaatcc
    22501 tgattacgac taaaaaaatg aacactattc cggaagattt acgaaatatt ctaatcaaaa
    22561 aaaccaagga aaatacacgc cagatcgtgc tggctggtag gcaggagaat ttagagtcta
    22621 tccagattct taaggaaaat ggtatgcaac tcacaaacct gaaaagcgac gcggccgccg
    22681 aattcatcga ggccggagta cgaacgagag accgattggc tggtgttttg tattctcgtg
    22741 aaatgctcga ccgggttctg gcgcttttag atgaatatcg cgctgagcat gctgaataaa
    22801 attagaacta taatcgaaaa atttgtcacc gccttagcgg ttgtcttgat gctattttta
    22861 gttgtaatgg ctttcctgca ggtaattttg cgcaattttt ttgcgattgg gttaaatgtt
    22921 gtggatgagt tgatgcgcaa cggagttttg tggatcgcct tctgtggggc ggcattaacg
    22981 accttacgta gtaaacacat ttcgattgac attctgccgc gtattgtaaa aggcaaagct
    23041 cgcttagtgt taaactggat cctcaatcta acagcggcgg cgatttgcct gtgtctcagc
    23101 tggtatgcct ggcaattctt gcgtttagaa ataagtatga attcgatgat tgccggagtt
    23161 attccagcct ggattatcga aattattctg ccggttggat ttttcctgct ggcattgact
    23221 tttcttctgc gtttaatgga taaatcccaa aatctcgaaa attaatggct ctgttgtttt
    23281 tagttctgat agttgttttg attttgtgcg gattgccgct cttcgcggca attggcataa
    23341 ccggcattat tcaatttgcc atttcgggaa ttgatccaac cgttgtcatc gtcgaaatgt
    23401 accgagtggc ttcggcgccg actcttctgg caattccctt attcacgtta gctggctacg
    23461 ttttagctga atgcagtgca ccgcgaaggc ttttgaactt agcggaggct ttgttaggtt
    23521 ggatacccgg cggcgttgca attggctcac ttgtaacttg tgctcttttt acctgcttca
    23581 ccggtgcgtc cgggattact atcatcgcct taggcggttt gattctacca attttgctta
    23641 aaaataacta ttcagagaaa tttgctctgg gactgattac cagttccggc tcgctcggat
    23701 tattatttcc ccccagtttg cctttgattt tatatggtgt tgtagcttcg gtttcgatcg
    23761 atcagctttt tattgcgggg cttattcctg gaattctatt gattatcctg ctctctattt
    23821 atgctgtccg taatgatgtt ttaaaaaaga gcgaacgaat acctttttcc tggcagaatt
    23881 tagggcgagc cctcaaagaa acggtctggg aattgccgat accggtcatt atcttgggtg
    23941 gaatttacgg cggatttata acggcggtgg aagcttcggc tgtaactgct ttttatgtat
    24001 tgatagcgga attcatcctg aaccgtgatt tatccttccg gcgggatttg atccgcatta
    24061 tgcgcgatag tgtgcaattg gtaggcggga ttttgattat tttaagttgt tcgatgggag
    24121 caacggctta tctaattgac gccgagattc cccagaaagc tctgctctgg gctcagcagg
    24181 ttttttcttc taaaataacc tttttgttag tattgaatat ttttctatta atcactggga
    24241 tgatgctcga tattttctcc gccattatta ttgtcgtgcc gctgattgtt ccgattgctc
    24301 tcagttatgg cattaacccg gtacatctcg gaattatttt tctcactaat cttgaaattg
    24361 gttacttgac tccgccaatt ggattgaatt tgttcatttc cagctatcgg tttgataaat
    24421 ccataacgga actttatcgg atttctctgc cgtttctatt tattatgcta attgctttag
    24481 ttctgattac ttatattccg gatttgagtt tatttttggt cggattgata caataaataa
    24541 ttcgtagaac agcctttcca ggctgtctaa taaacattac ctgtccttaa catagacagg
    24601 caagaatgcc tgtcctacaa ttatttttat caatctaaat ggtacgaaat cgaaaaaaat
    24661 gcattgcggg tagcaccagg ccaataataa gcatagtatt caccccattc atagccatag
    24721 ccataagtcg agtaaagccg gttgaataag ttattgactt tcgcgccgat ttcaacccga
    24781 ccgaatgaat atctcagtcc gacatcagtt agccaataag ccggattcat tccgatattg
    24841 gcttcgtcaa tatattgttc gccaacataa cggataatcg ccgaaatcgn nnnnnnnnnn
    24901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ccgacgcctg tgtatacaca tattcacctt
    25021 cctgctcgat atcaatattt tttaactgtt catttttata atttatccgg taaagattca
    25081 aatccgctac cagctttcga cctcgatagt tggcgcccag ttcgtaatca tcaatcaact
    25141 ccgcggcggc tttttgaggt tcgctccaat aatcgtcggc ttcaataatt tgttcatcgg
    25201 ctggttcttt ttgcgagcgt ccgtaattag caaaaattga aaagtcattt gatagttcat
    25261 atatagctcc aaatcgagga ttcaggaaat cccacttagc ggaaatatca tacccaatcg
    25321 cataaccgat ttttttctga tccagattcc attgcaagtg ttgatattgc aaatcagtga
    25381 tcaatttcaa tttctttagc ggttcaagcg taaagtgaac aaagccggtt gcaattgact
    25441 tttcaccgat ataacgataa tagcgatacc agcctttccc aatttgcgcg ttcaaattct
    25501 gatcggaaaa tcgggaaatt tcaccaaaat gattgccgtt atagtattta aattccccgc
    25561 cgatatcgag gcgatactta gctttgataa atgtcaaagt cggtacgatt ccgtaatacg
    25621 aattcacaat ccatttacgg cgggttaatt ccagtgacag gctgtcagca tcagtcgggt
    25681 aatattgatc aaggttaaaa gaacgcaaaa aatcccgaaa ttcatctcgg gcagttgtat
    25741 cgtcaaaagc taagttatat gccgctgatt tttctgactc gtaatagcca tcacctttca
    25801 ccagataagc aacattattg aaattaagat tctcgctaat ccgccaggtg ctattcaagg
    25861 aataaacctg ctgaagaaaa ttatctatat acgatttgta accggaacga cgtccgatcc
    25921 gatcattgat caattcggct ggaacgccat cccacatcaa gttacttttt tggtaaccaa
    25981 ccagcgcccg aaattgatga gaagtatttt gtccgttata ttctataccg gcagaaccgg
    26041 aacgctgaaa ggtttcatgg tattcacgat agccgtctga ttctatctgc gagagacggg
    26101 ctgaaaggcg tatctttcca ttgtgtaact gcccggagga agcacccaga cgtattttcg
    26161 aagtattata cgaaccgccg cctaaatcaa ttgaaaacgt cggtcggtcg gtcccaatcg
    26221 tggtcaagac attgatcgaa ccaccgaaag ccgacgatcc gtataagcta ttgccgatac
    26281 cgcgttgaat ctggacatca gccgcgtcag aaagaatatc accgtgatca acccaataaa
    26341 cctgatgact ttcattatca ttcagaggaa cgttatttaa catcacggca atccgactct
    26401 ggtcaaagcc acgaatctgg acgtaggaat agccggtacc attgccgctc tcactatagg
    26461 aataaacgcc gggttctata gccaaaatca tcggtacatc ttctacggtg tagcgacttt
    26521 tgatttcctc aatggataat tccgaaaaag ccaccggtgt cttgcctttg actgcccgcg
    26581 tggccgtaac gaaaacatac tcgccggtaa ttacagtcgg ctttaagtaa atcgtaccgc
    26641 aatcttcgga agtagctgaa aaagacgtat cccggaaagc aatgtgagta acagtcactt
    26701 ttatcggaag aaacgatgtt ttgatgcgaa aattgccgtc cttgtccgaa aatgtaccgg
    26761 agccatcttc cagcaaaata ttagccgatt cgactggctg accggttgtt tcggatacta
    26821 attttccaaa aattaaatgt tcttcaccga aagcgtttgc ctcggttaaa ataagaatga
    26881 aaagaatgaa gaaaaataca ggtaggtttt tttgatgcat aattactccc ttcgctggta
    26941 ttatccagat caggttcaaa gggtaaattc tcagccgccc cgtcaaacgg gttagcaccc
    27001 ccggaccatc acttgttaaa aatcactgga aattaataac tcgaaaaatc agacgcaact
    27061 aatttttgac tttgcggaaa tgcctgggag tcgaacccag cttcccgatt agatcgggaa
    27121 cgacggtttt gaagaccgcg gcccccaccg gggtagcatt cattcccata ttcattgttt
    27181 gacaacaata aaatctaagt tatgatcttt tttcaattta tttccaaccg cgcgcgcctc
    27241 ctcttccgac ttgaatgaac cagccagaac cgccgtcaac ttgcggccgg atctttctat
    27301 ttggcgcaaa gtaaccgaat accccagttt ctctaattta cttcgaagcg ccctagcgtt
    27361 tgctggatca ccaaaaacac ccatttgcaa agtaaagttg ccttgtcctg gcacttctac
    27421 ttttggcaga gaataggttt gagtctccgc cgatttagcg ttagaagata tttgactgct
    27481 ggtcggttga gattgtttct tagattcgat actagtaacc ttagaagcga ttttgtaatc
    27541 ctcaaaatga aaatttaggt tagggtactg agtcgaataa tacttataat aataatcaac
    27601 tgaatcccgt ttattaaggg cgttaaaact acataatcga agataaattg ctttgtcaat
    27661 caaatccgat tgcggatagg ttttgatcaa ttcactggcg tatttttccg ttttagtata
    27721 caagccttta gtaaataaat attcgataat tttcagtaaa gcatcgtcag caaccgagct
    27781 gctggggaaa cgactgagca catcacgata aattagcaaa gccgcttcac catcggtttc
    27841 aatcattcca ctaatataaa gcaactcggg attatcggga tattcagcta ttaatgcagg
    27901 caagagtgtt ctgacggaat caacttcgcc cgcccgtatt ttctgcttca attccccgat
    27961 attttgggca aatataaaag ccggaatcaa tatgagaaaa acgaatcgtt tcatacgtcc
    28021 gtaaaatagg tatcagccaa accgcaaacc ggacaaatcc acagaatatc agaactccga
    28081 ttaccgcaat tagagcagat atactctttc tgatcaacca ttttttcaat tatgtcattt
    28141 agaatggctg tagctcgatt taattcggcc tgtccaacaa gcagtttagc cagattaatt
    28201 ttggcgatgg accagccggg atttttttcg actaaatctt ccagaatagc gatagctttt
    28261 tttgcatttc ccttcttttc gtaaaaatca gcgagagcaa ttaaggtttt gggatcatcg
    28321 ggtattcttc gtaggagatt ttcataaaaa ctttcaatat tgccaaaatt cccgagatta
    28381 aataaaatcg tctgcagagg tttaaaaatc aaataggctt tgtgcggtgc tacgtcggta
    28441 aatttaaccc actcttcgac ggcatcttct tcccgatgat ccagaacata agatttacca
    28501 atataataat aagccgcctc acaatttgga tcaattttta gcgctttccg aaaaattaaa
    28561 cgggcttcat ggtattcacc cgcctggtat ttgactaaac cttcttgaac tttataaaga
    28621 gccagaagcc gatcgttacg ttcttttttt aattccaaag ccatcgccag atattcagat
    28681 gctttctccc agcgtcctaa gtcccggttc aaaatatgat tcttttctaa agcccagata
    28741 tttttcttat caatactcag aatgttctcg acataattta gcgcgcgaat tttatcacca
    28801 gcggcaatgt aatcttccac taaattacgc agtatttcca gacgttggat attactaatt
    28861 tcctgccgat agagaagcga ttcatgaatt tttatagcgt ttttaggctt accattcttg
    28921 cgcatcagaa tacccaaatt gatataagcg tcaatattat cggtgtctgc ccggatgaca
    28981 tcccgaaaca agcggattgc gacatcatcc tcgccgtgta acatatgatt gagcgccata
    29041 gcataaaggc tggcggaatc tttggaccgg cttagacgac gccgaccagt cagattaatt
    29101 acgataaatc caataagcgc aatgagaacc agcgttaaaa gtatatccaa taaagacata
    29161 ttaattcctg tttattcggt ttttgaatta tcggcttctg tttcgtcaat tccctgattt
    29221 cgcagtaaat caacttcctt cttcaacttt ttataatcgt tcgataaagc gcgtagttca
    29281 ttcttagtgg taagaatttt aataccagca attaaaaagc caattacaag tcccagtata
    29341 aaagtcaaaa ataccagaat tagaatatcc atctcaactg gagatgcttt ccagaaaaac
    29401 tggattttca ccggtggatt caaatttgag ttctgaataa caaaaaacac gattaaaaac
    29461 atgatcaaaa gcgttacaac ccagcgtaaa aatttcataa tcacctcaaa atttaaatga
    29521 ttttaccaaa taaagacact cccatagcct tttcgatttg gacattacaa aactcaccga
    29581 tttttggatg tccttctttt atcacgacga ttttattaga ttcggtacgg ccgatcattt
    29641 cgtccggatt tttcttactg gttccgtcaa ttaatacctc ttgaaccgta cccactaagg
    29701 ctttattacg ttccaatgta tgacttttct gaagttgaat aacggctttc agacgacgag
    29761 atttttcctc ctccgggaca tcgtcattca ttttagccgc tttagttccc gggcgcggag
    29821 aatatttgaa cataaaggct gagtcgaact tgattgtctg cattacctgc aaagtctgtt
    29881 caaaatcatc tgcggtttct cccggaaaac caacaataat atcggtcgta agcgcaacct
    29941 gagggagaaa ttctctaatt aagttaacta ctttcagata atgcacttga tcataggaac
    30001 ggttcattaa cttcagaatc cgggatgagc cggcttgaag aggcaaatga atctgtttac
    30061 aaatattaga atgagattgc ataactgcta acattttttc atcaacgtcg ccgggatggg
    30121 gcgaggtaaa acgaatccga cgcaaatccg gtacagcggc aactacctca agcaattccg
    30181 gaaagcgata tgcttcataa tggtaggaat tgacattttg ccccagcaag gttacttcgc
    30241 ggtaaccgtc atctaatgct tgtcgaattt catcgataat gctctgcggt gaacgactgc
    30301 gttccctccc gcgtacatac ggcacgatac aaaaagtgca aaatttgtta cagccgcgca
    30361 taatcgagac ccaggcattg accgcgctat tgcgagaagg aattagctca tcatatactt
    30421 ccgtgcgcga tagattcaca tccacgatag gcatctgttc ctgattcaaa tattccggta
    30481 aattccgata ggaatctggg cctaaaacta aatccacaaa tttgttttca gtcaggattt
    30541 cgtcatgagc tcgctgagcg acacatccga tcagtccgag caacagctcc ggtttctttt
    30601 tctttagact tttaaattgc gctaatctgg caatcgcccg cttttccgca ttttctcgga
    30661 cactacaaga attaatgaga attaaatcag cctcttcggg aatagccgtc agctgataat
    30721 tagctccgcg cagtaagcca ataaccaact cggaatcagc ttcattcatt tgacagccat
    30781 aggtttcgat ataaaatttt cgggcttcca tcgtaactgc tgaaatttaa aatataaaac
    30841 cggttttgga aatgaaaact aagaaattta tatgagggaa tgaatctacg cttaacgaat
    30901 atatccggag aagaaaaagt caagcggatt tttattgata ttaaactgct ttacttcgta
    30961 gtgcaaatgc ggtccggtcg tgcggccggt agtgccgacc tcgccaattt tttggccgcg
    31021 tttaacggct tcaccagatc gaactaatat tttcgaaaga tgagcataga gagttgaata
    31081 tccgtagcca tgattgactt cgaccacgta accatatcca ccttgataac cggatttacc
    31141 gacgactcca tcagccgtca caaaaactgg agtcccagtt ggcgtcgtaa tatctaaacc
    31201 ataatggaac tcacgccgac cggtaaacgg atctcttcgg taaccaaaac catcattgat
    31261 aaaaccggta taaaccggtc gaatggacgg agtggactga atctgatcgg aacgattttt
    31321 gaaagcatca taaatcgttt cataactgat gcgctcaaat ttaatttccc gcgccaggcg
    31381 atccagatta ttgactaatt ccgaaacttt tatatcactc tcaggcagga gctgatctaa
    31441 ttcgtccgat ttgggcatta aacgaccacc aacacccagc cggcggatgt cctgatcaat
    31501 tacggggaga tcaccgtagg ttcggagagc cttgtctttt tcaaccagca tgctaatatc
    31561 cgattccatt ttctggatgc gattattcaa gtcgtaaaag gttgaaacca accgattatt
    31621 gtttttctta agacgagtta acctggattg ataaaccaga ttagaaaaca aattggcgcc
    31681 taaaataatg acaaaaataa ggagaaaaac tccaccggcg agaacggcaa ccaactttcc
    31741 ccaactgagc gaaatcgaat gcacccgttt tcgtgtgttc actacaaaaa ggagtttggg
    31801 tgcttttttc attttgttag tttatttttc gcccatatta taaaattcat tgtgaatttt
    31861 aaaaaatagt ttttattaag tcaatatttt ttttagggaa ttattaatta agtaaatctc
    31921 acttcgggtt tgctcagatt atcaccatca cttaattgca attccggcaa gcaattatgc
    31981 cataaataac tttacgaacc gacttttaaa atacctataa aaatttggtt aaaaattgga
    32041 tagttagtct aaaaaattat tgctttttct taaaatcttt ctcatatttt ggccgttgga
    32101 tgtaactgtg aatatctgat acgaacgatt agagagcggt ttttaggaat aattctcgga
    32161 agatattcat tctatagtaa atatttttta aaattacaaa ttaaggagga aagtatgaaa
    32221 aaaggtggct atgttttaat aatgatagcg gtgctgagca cgatggcgga ggtggggtgg
    32281 ggacaggtaa gtattactca atttgaaact gcatacaatc aagatttcaa ttcattagct
    32341 aatactaaaa cggataatgc ctggaccgat aattccacta tcagcgggtg gtattcaaac
    32401 aggacagagt acatcgccga taatggtagt agtacaactg gcggtttaca tagctatgga
    32461 tcggcggatg ccagtgagag agcattaggt ggactttcat ctagttcggc ttcccccgct
    32521 tttggtattc gtttagtaaa taacactgga aacactatta cttcatttaa tatttcctat
    32581 aaaggggagc aatggaggca aacggcgaat tctcatacat tagttttttc ttatcaagta
    32641 gatgcaacag gaattgaatc tggaacatgg acatcttttt cagatttaga ttttacagct
    32701 ttgaaaactg gtacctctag cgctcttgat ggtaatgctg aaggtaatta ccaaaatgta
    32761 tcatcaaatg ttatcattga agtacctaac ggacatgaaa tatggtttcg ttggctaaaa
    32821 actggaacta atagtcctgg tttagctatt gacgatttat ctataacagc ttatgaaata
    32881 aaagtaccta ctatatcggt aaatcctagt agtctgtcag gtttttctta tgtcgttggt
    32941 tctggcccat ctgccgagca gacttttaca gttagcggta cggatctaac ggcagacata
    33001 tcaatttcgg caccagaaca ctttgaaatt tcaaaaacaa gtggttcgga tttcacctca
    33061 tcgcttactt ttgctcaatc agacggttcc gtgaacgaaa ccattatcta tgtgcgatta
    33121 aaagccggct tggcaattgg ctcatatagt tccgaaaata ttacctgctc ttctgagggg
    33181 gccgacgata gaactgtaac ctgcagtggc actgtgacta gtccaccgat gcattatcga
    33241 agtaaaatta ctgggaattg gggagataat tctacctggg aatcatcaac ggatcaaacg
    33301 acctggagca atgcatctct atctcctaca tcgaacgatt ataccataac aattcaaaac
    33361 ggacacacg
//
LOCUS       DAOOMU010000013        32165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1173_length_32165_cov_21.937434, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000013 DAOOMU010000000
VERSION     DAOOMU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 32165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32165
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1173_length_32165_cov_21.937434"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..749
                     /locus_tag="PLI55_02285"
     CDS             117..749
                     /locus_tag="PLI55_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPA99696.1"
                     /translation="MTVLMHAVTPPRFAAGSLHSKAIFQSKKMENEYKIYEHNPPHLF
                     CPGSKYIITGATYGKKPWLKSDEAKERLLNSIKKGFDHYHWTLEDWVILDNHYHLIGN
                     APEDASTLSLMINDIYRFIALWIKKNVPESKNDKIAMYNYWDTCITYEKSYLARLNYI
                     WYNPMKHGYVEDTSDWKFGSYYERIKVEKEEMEELRKKYKWDKVKVKDDF"
     gene            938..1072
                     /gene="rpmH"
                     /locus_tag="PLI55_02290"
     CDS             938..1072
                     /gene="rpmH"
                     /locus_tag="PLI55_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017471599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPA99697.1"
                     /translation="MKRTYQPSNRKRKNDHGFRKRMSTNSGRKVIARRRAKGRKVLSA
                     "
     gene            1014..1388
                     /locus_tag="PLI55_02295"
     CDS             1014..1388
                     /locus_tag="PLI55_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPA99698.1"
                     /translation="MAEKSSPEDELKDVRSYRPNLPRRERLAKWDQRAQIIHNGKPLK
                     YDCLYISILPQDDWKLSIVIKKECGNAVQRNLYKRKIREAFRLCKPYCKRPAAIAVTV
                     FKKPDNLQVNTLSEILINNLNL"
     gene            1394..1624
                     /gene="yidD"
                     /locus_tag="PLI55_02300"
     CDS             1394..1624
                     /gene="yidD"
                     /locus_tag="PLI55_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPA99699.1"
                     /translation="MKIFNILIILIIKTYRLLLSPLFPNTCRFTPTCSQYALEAFKKF
                     NFFKASWLSLTRILKCHPYHTGGYDPLPTPKE"
     gene            1627..3387
                     /gene="yidC"
                     /locus_tag="PLI55_02305"
     CDS             1627..3387
                     /gene="yidC"
                     /locus_tag="PLI55_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPA99700.1"
                     /translation="MDRKSLIGFVLIFVIILTMPYYFKIISKNSPEVTQEKVIEKTPE
                     SVISETIPAQLSESHPSSEVQRPASLGSEKPDTLTIENDLYRLKISSRGGGTITSVVL
                     KNYNKIIGRDTSLVEMIPPFEARRLFIKYIKSNGDSVTIDQNFRIFTEHQETSKNYYK
                     ISDGDSIIIQFQLPEGDNIIVSRTFVFYGDKYIINSYNDLGGILDEMASDKYDLVWEG
                     GIAYTEAMIKEDVSYSRASACGGGETENLDVKPGKTGSASLVGATYWTAIKTKYFVAA
                     LIPHQPAEGYRLTGLGVPLQGKDFQKIFNMYLTLNASSENAVKIYLGPLDYSIIKGLA
                     PNFEHLMNFGFSLIRPISKGILWLFTKMHLFIPNYGWVLIIFSVLLKIVLTPLTNSST
                     RSMKEMQNIQPQLNALQEKYASDPQRLNNERLKLMREHGVNPMGGCLPLLLQMPILWA
                     LFIVFRTTIELRQAPFILWITDLSAPDTIFTLPFSIPIYGSQVNVLPFIMAISQLVQQ
                     KISGASGGKQQKVMMYFMTIFFFFLFNQFPSGLNLYYTLYNILSIIQQKYFPPKPKPK
                     KPHKTNSQRLRQYQTRMRGR"
     gene            3407..4759
                     /gene="mnmE"
                     /locus_tag="PLI55_02310"
     CDS             3407..4759
                     /gene="mnmE"
                     /locus_tag="PLI55_02310"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPA99701.1"
                     /translation="MSTENTIVALSTPPGRGALAVVRLSGPKSPDIVHKLTKTTTQLK
                     PRVAQHLNLYKNGELLDDAVIIYYKAPMSYTGEDLVEISCHGSPFIVNQLIELCLRYG
                     ARLAQPGEFTRRAFLNGKFDLTQAEAVADLVFSQTQASHQAAIKSLEGTIGKEFAELR
                     SKIIELITILELELDFSDHEIDTIPPHELSKSIDNILKINSKLLKSYSCGKILKQGAL
                     VPIIGPPNSGKSSLLNAFLNEERAIVTPYPGTTRDSLEEAIQAGGYMIRLLDTAGIRK
                     TKNPIEKIGIDRTKKNIKTGDIILFVIDATQPIKREWLEYYNSKNTIVVINKIDIAPQ
                     KSLTKYEKHFSQYPHIFVSAKKRQGIHKLTEMIVDSISQQIGTADSLIITNQRHATGL
                     RTAKKYLLSAQKHIRQSLAPEMIIADLRIALNEYDKILGKTSNDEILNNIFQHFCIGK
                     "
     gene            4769..6649
                     /gene="mnmG"
                     /locus_tag="PLI55_02315"
     CDS             4769..6649
                     /gene="mnmG"
                     /locus_tag="PLI55_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPA99702.1"
                     /translation="MKHLDFDITVVGGGHAGIEAALAAARLGLSTLLVTQNISTIGRM
                     SCNPSIGGLAKGHLVAEIDALGGAMAKLIDRTGIQFKMLNKSKGRAVWSPRAQADKIH
                     YSLLARNTLSNQDRLTLLEDTASGLIVDKQIIQGIHLIRNGDIFCQAVILTCGTFLNG
                     LIHVGLQQFHGGRIDEEPVSGLTEDLARYNIYSGRLKTGTPPRVLAESIDFSRTMPQY
                     GDQDPIPFSFSTQNFSPPNIPCFLTRTNTVTHNFILNNLDRSPLYSGIIKGIGPRYCP
                     SIEDKCVRFKERTSHQIFLEPEWKNSNEYYVNGFSTSLPLDVQMKALRTVPGLEKAEF
                     IKPGYAVEYDFFPSYQIKDTLETKSLNGLYIAGQINGTSGYEEAAALGLMAGINAALS
                     IQGREPFVLQRHEAYIGVLIDDLITKSPNEPYRMFTSSAEHRLMLRFDNADRRLARKG
                     YELGLLPRETYESTQHKIEVIEEIISFLSSTNISPEAAKVILPNNDAHSIGCNLSLYK
                     LLCRPDVALASLVPFLSSEIKNKIAHFPKIIEEIECDVKYAGYIKHSQALIESMSKNE
                     NIKIPPDFDYSAVRSLTNEAREKLIRFRPQTLGQASRIPGVSPADVAALFFLLHKHSG
                     LT"
     gene            6646..9879
                     /gene="mfd"
                     /locus_tag="PLI55_02320"
     CDS             6646..9879
                     /gene="mfd"
                     /locus_tag="PLI55_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPA99703.1"
                     /translation="MKHLDDRIENLVSTFSESSAFLNAVKAFYRSNAGFTVAGLSGFA
                     TALFLYSFYKKIDQPILLITNDSNEAEYIRDDLELFNLDDSVCYLPEPPNSSFLFSDT
                     ESLNAYFTNDTLQKLSTGQNTIIVATLSSLKSKFPLSDDFNKLKRTYHVNEIVNRDAF
                     IEYLVHLGYTIAPAVESPFEYCIKGGIIDFYPPESLHPYRIEFFGDKIESIRTFNPDT
                     QLSICNLSSFTVQAPAHSSLDNQNSTNIFSYLKKDTVVFCLHAEKLMDTDEKNTTDIF
                     TYLSSYRVINHYDLLASDVDFRVNPQPQTNSIKAFREHLLTVLSAQPDARISLFCANS
                     MQVERLKELLGLEKLNYFDFSISAGAELPEAKLYFYTDHQIFKRERRINFFKKYSPDF
                     PVEQFSPHEISPGDLMVHINYGVGRFAGLDKIAAFGSIRECLCLEYSGGDKVFVPLEK
                     LNYVQKYTGNLQNPPPLNKLGTTEWERTKLKTQRAIDEISKDLVELYAKRISAPGYAF
                     PPDNELQYLMESEFLYEETPDQLTAIQAVKEDMEKPRPMDRLLCGDVGFGKTEVAIRA
                     SFKAVSASKQVAVLVPTTILADQHFAVFSQRLQKYPIRIALLSRFVSRKMQSQIIRDL
                     AAGKIDIVIGTHRLLSKDVSFKDLGLLVIDEEHRFGVKDKERIKFLRESVDILALSAT
                     PIPRSLNFSLIGVRDFSVINTPPISRLPVITEVITFDMNIIKKAILREINRGGQVFFV
                     HNDIKSIAALSHKLALALPEISVDYVHGQMNERELEKTMSAFINNEINLLVTTTIIES
                     GIDIPNANTIFINQAHHFGLAQLYQLRGRVGRSSRRAYAYLIVPEPSRIKPDALRKLQ
                     TIKKHTALGSGYSVALQDLEIRGAGNLLGTSQSGNNIAAIGYELYMKFLREALDEIKS
                     TQPAYPEKLKLETEVFCPLPAYFPDSYISDSSARLEYYRQLTLADELSTVDLISGQIR
                     DIYGKLPSEATTLFNISKIRLLASSLGIKKVNIAEKSTSFIWDESFIPPAETDLIQAI
                     QKAASDLGLSYKFNPYNTLYLTVYLKSNFDFDKITRFLNLLRNTLNL"
     gene            10026..10850
                     /locus_tag="PLI55_02325"
     CDS             10026..10850
                     /locus_tag="PLI55_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPA99704.1"
                     /translation="MRKDILLMIFIFVWAACGNHKEDYIARVGNTYLTNEDLTRMLPD
                     GGLSKDIDKNYLNSVVSNWINKEILYQKARQYHFDREEGVQARVADYYRDLTIDSYVR
                     FFLQTNVTISEDEIRDYYLKNKKSFIRDREEAKIVHVLVQDFNDALAIKSAITSRNKN
                     ALDAFYSKYKFETSVVKRGESLEEIDRNIFETSPRSIIGPIASNYGFHIVEVLARYPI
                     GSTRTIDEVRDEIYQILTQKKIQDQYNALVDSLVRNANYEIKDENISNFLNSIGSR"
     gene            10847..12106
                     /locus_tag="PLI55_02330"
     CDS             10847..12106
                     /locus_tag="PLI55_02330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPA99705.1"
                     /translation="MKIKLFFGIIFFATTLFAQTLIDGIAAIVGENIILISEVDQVAR
                     ITASQMNIDPNRDVANFNKIKRHVLNSLIDENVILEQARIETVEVKERDVENAINQRL
                     DMLVEQVGSVENLELLMGSPISKIKKDYRPIFKNQLIVEKMRSEKFSKVTVTRPEVEA
                     FYQTYKDSIPPIPPSLDFSAILFKVTPGPEEEMAAKKLIDSLYTLLKNGYDFEYLAKN
                     YSEDEASAPYGGDLGYIKRGAFIKSFEEVAFSLSPGEISDVIKTDFGYHIIQMVDRKG
                     ESIRVRHILIKIKVSDKNYEDRYRFADSIRTMIINNEISFDSAVVKYSDEPNAKSTLG
                     RIRRIPLNQIQNTDFINVLSNMKVGDISPVFESSPGYYILKLNNVYDDSWPTIERFAL
                     EFKKNQLYQEWLNKLKEDIFIDIKVNY"
     gene            12427..13542
                     /gene="dnaN"
                     /locus_tag="PLI55_02335"
     CDS             12427..13542
                     /gene="dnaN"
                     /locus_tag="PLI55_02335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPA99706.1"
                     /translation="MQFSVSQNLLLNNLNKVAKVTPARTTMPILNSILFILKDSNLTL
                     RASDIEITLSTTFPVNGQEDGSVAVPSRLIQEIVGEIREGELTFNATDEGEIKITAGK
                     GKYKIMGRPGEEFPALPSIINQNSILVNSKILSRIIQKTVFAVSRDELKPSLTGVLFE
                     VKADELRSVATDGHRLVYINRKDFSSPDYERQIIVPVKFLNLINTYLTDDNKVSLTIS
                     DNHIMMESDSTLIISRIIDERFPDYESVIPKDNDKELKADSDYLSAVLKRVSIFSNKS
                     THQISLSLNRELSKISTIDLETMSSADEILDVEYSGDDLTIGFNAEYLREIVRNIDTQ
                     KVIMKLKTPVSASLVIPENQEENEDLIMLLMPIRLSD"
     gene            13578..14693
                     /gene="recF"
                     /locus_tag="PLI55_02340"
     CDS             13578..14693
                     /gene="recF"
                     /locus_tag="PLI55_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPA99707.1"
                     /translation="MVLKNLRLVNFRKYTDSVFVFHEKTNFINGDNGSGKTSLLEAIH
                     YLSLTKSFRTNNDSEAVNYQSDYFNIFGEFLSDNNQTILSNMNYSKTEGRSFLMNKNR
                     VKRYTDVVGCAPVVILSPGLQKITEGGPAERRNFINRIISQTDRNYLVTLIEYRRRIS
                     QRNVVLNQYREKKRCRYDAYFEAQDEILARLGQKLFIGRQAFIADMDSIFKTVFARIT
                     HIGGTVGIDLKYNIKADPDNFISLFTKNLRERFSRDLLIGRTTCGPHLDEIIINYNGR
                     DIRQIGSQGEHKLILIALKLAEGAYIESHQNEKIIFLLDDLFAFLDGKHCRKIVEEIG
                     DKNQALITSTDVKMLRKYGFLDNPEKYKIFNLTTGEA"
     gene            14696..14971
                     /locus_tag="PLI55_02345"
     CDS             14696..14971
                     /locus_tag="PLI55_02345"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPA99708.1"
                     /translation="MESLGTAIARMLKIYGIEKPVRQNEVFFIWQDIVGEVIAKHAIP
                     EKVAYNKLFVKVDSPVWRNELNFRKEEILKRINSQIKNAQIKEIILR"
     gene            14968..16899
                     /gene="gyrB"
                     /locus_tag="PLI55_02350"
     CDS             14968..16899
                     /gene="gyrB"
                     /locus_tag="PLI55_02350"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPA99709.1"
                     /translation="MSEIKFTDAAIDYGAEKITVLKGLDAVRKRPAMYIGDTGKRGLH
                     HLVFEVIDNSIDEALAGFCNKIVVTVHEDNSVSVLDNGRGIPIDIHKEEKKSALEVVM
                     TILHAGGKFDKGSYKVSGGLHGVGVSVVNALSEWMRVEVYRDGKIYTQSYSRGVPTSK
                     VEPVGKTNKHGTLVRFKADVEVFKKIEYEWEVLEHRCRELAFLNRDLEIVLVDERDKN
                     ENRFHYKGGLAEFVNFLDETRPAIIDKPITAEKEEDGVFVEFAMQYNTSYNENIFTFV
                     NNINTIEGGTHLVGFKTALTRTLNNYAQKNNLFKKESFQLSGDDVREGLTAVISVKVP
                     EPQFEGQTKTKLGNSEVKGIVDSIVSESLAEYLERNPTQGRRIIEKSLNAARSREAAR
                     KARELTRRKSALDIGNLPGKLADCSTRDPSMCELFLVEGDSAGGSAKQGRDRRYQAIL
                     PLRGKIINVEKTRIDRVLSNEEIRTIITALGTGFGDDEFDIQKLRYNKVIIMTDADVD
                     GSHIRTLLLTFFFRYLPKLIEDGHIYIAQPPLYKVVCKKQEYYAYDDEELEVLTKRFS
                     KDSTAKIDIQRYKGLGEMNPEQLWNTTMNPETRTLLKITIEEAAAADRTFSILMGGDV
                     EPRRQFIQKNAKYVKNLDI"
     gene            16903..19452
                     /gene="gyrA"
                     /locus_tag="PLI55_02355"
     CDS             16903..19452
                     /gene="gyrA"
                     /locus_tag="PLI55_02355"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017258646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPA99710.1"
                     /translation="MASQVNIEFQRDKIVPVNIDEEMRSSYLDYSMSVIVARALPDVR
                     DGLKPVHRRILYGMSELGLTYNRPTKKCARIVGEVLGKYHPHGDMAVYDALVRMAQPF
                     SMRYPLVDGQGNFGSLDGDSAAAMRYTEARMSRIASELLRDIDKETVKFVPNFDESLE
                     EPSVLPTAVPVILVNGSSGIAVGMATNIPPHNLTEVCNAAIYVIDHPEATVDELMQFI
                     PGPDFPTGAFILGKSEIKRAYETGKGVIKMRAQAFVENSKTGKESIIITEIPYQVNKS
                     NLIEKIADLVREKVIDGITDIRDESDKEGIRIVIELRRDAYAEVILNQLYNHTQLQES
                     FGINMLALVNGVPRQLSLREILQHFIDFRHEIIIKRTQFDLRQAEDKAHILEGLKIAL
                     DNIDEVIAIIKASKSPEDAKQNLMIRFRFSERQAQAILDMRLQRLTGLEVNKVIEEYK
                     ETLKLIERLKFILSNVSEQMLIIKNELAEVKEKYGDARRTQIIADEGEFSVEDMIAEE
                     DMVITITHNGFIKRFPVDASRRQNRGGRGSLGAQAKDDDFVRNLFIANTHDYLLVFTN
                     KGRCYWLKVHEIPRAGKGSRGRAIVNLINTQPGEEVRAVLGVKEFDDQHFVLMVTRNG
                     IVKKTVLSEFGNPRKPGIRAINLNDNDDLLDVQITNGNQDIILGTAYGKSIRFHESDV
                     RPMGRTAAGVRGVRLSGKDDRVVGVVIVRNESGTILAVAENGYGKRSDIKEYPVIRRG
                     GKGVITLKTSVRNGRMVALREVVDTDDLMIITAKGVLIRLPVATLRTISRNTQGVRLI
                     KLDEGDTISAVTRVVESEDKNNGNGELEESDEITLLENDASDEVAETDEPE"
     gene            19477..20184
                     /locus_tag="PLI55_02360"
     CDS             19477..20184
                     /locus_tag="PLI55_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPA99711.1"
                     /translation="MDSQTIQNNLLIIKERIVQAAQKTGRRAEDIQIVAVTKTFPATL
                     VRQAYTAGLRIFGENRVQEAIPKIIALQDCPEISWHMIGHLQSNKVRKAIENFAMIQS
                     VDSLDLCNKIANISRALGRKVDILLEVNVSGEATKSGLPPGKVFSVVEQVAEMPELIL
                     HGLMTIGPLTNDRDWTRRAFANLKGIFDKIAESRINTGQFKLISMGMTDDYEIAIEEG
                     SNMIRLGRAIFGERRTS"
     gene            20181..20627
                     /locus_tag="PLI55_02365"
     CDS             20181..20627
                     /locus_tag="PLI55_02365"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPA99712.1"
                     /translation="MIKTDEIRDRRFRKVFRGYDPVEVKYFTEMLADEFAQMEEKLEK
                     LEAIVNEFDQTRKGRDKILQEAQDRAASIILDAESIASEVIANAETKKRQIQEAIDQL
                     NHEQNKLIQRLRSVLKNQAEILTWLEEAPDWSEEDNTLNESSEETT"
     gene            20667..21227
                     /locus_tag="PLI55_02370"
     CDS             20667..21227
                     /locus_tag="PLI55_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99713.1"
                     /translation="MNKAVCPQIPEGELNFKIILLDNGANVKTWREAVRSAIKDGAER
                     FLLVGRGVPTRRSEKSAGIVVVDQINVSGQNPLVGPNDEEFGTRFPDMTDLYSPKLTE
                     DVLIAGKQAGLVLQKGILLVPVSMTSSTSLEEKLIVQNEIAALSQDVFAGAITVRHAG
                     RQCTGIILFSDIDGKQLGLLLAHLDW"
     gene            21307..>21598
                     /locus_tag="PLI55_02375"
     CDS             21307..>21598
                     /locus_tag="PLI55_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99714.1"
                     /translation="MIQRIRNIYVHRFRVFGFLLLILPNIVWAGHPVTIDKFTNQPTI
                     RAWIYLSNEPAYNEYFTQLEYPEKTQRRLAKMGTLAKKTASDLVPGEELRAII"
     assembly_gap    21599..21698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21699..22898
                     /locus_tag="PLI55_02380"
     CDS             <21699..22898
                     /locus_tag="PLI55_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPA99715.1"
                     /translation="VRIGFIDTGFFKDHEVFSEILSSGRLIAEYDFINDDDNVSDEEA
                     ADTARNSRQSEHGTAVWSLVGGYNPPDYLGAAYGAEFLLAKTERIDTEIHREEDDFVA
                     AVEWCDYWGADIVSVSLGYRDGFADGSKYPYEYLDGQTAVTTKAVNWAFERGILCVCC
                     AGNDANNFEDGGLLTPGDAFGALTVGAVDAQGEIASFSSHGPTYDGRIKPDLCAMGVA
                     TYYASNSQGAYRTGSGTSLATPLIAGAAALLLESQPELTPAEIITELKRYADRTQNPD
                     NRYGWGIPDVYQSIVNYQPKSFPKGELSFDEIWVYPNPANSNINFYFIWHKATPATAP
                     AYLQVINLNGQIVFEKRLVSKFGGQKEVINWSLHNQSGNRVSSGIYFVCLQNPEYFKI
                     SKFIIMR"
     gene            22950..23504
                     /locus_tag="PLI55_02385"
     CDS             22950..23504
                     /locus_tag="PLI55_02385"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPA99716.1"
                     /translation="MEKEEVELIQNIQSGDKMAFARLMEQYGERVMSIAFRFTRNYQD
                     AEDLYQETFIKVYQNLGKFRFESEFFTWIYRILANQAFNLYNKRKRMTLADPGEDDYL
                     WETIPADAQDEADRVTFSNSIQDQIDAALQQLSAKQRVVFILKHFEGKKIKDIAILLD
                     CTEGTVKRYLFRATHKLQTLLTES"
     gene            23519..24550
                     /locus_tag="PLI55_02390"
     CDS             23519..24550
                     /locus_tag="PLI55_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99717.1"
                     /translation="MKHDKHFKELSILYFYNELSESERTDFKHHLDECQVCQAEIERL
                     QIIAKKIDYATEPVPSARRLEALNKQVISKISSESRRPKLVNVKTYFEEMFETITIGL
                     SRPRYQLIAASLILVIGIFIGRVGFRADLPKQPEILANFVNYNYKFTDPEKENIQKAF
                     TNYLLKSGGIEDADLLQSEYRTSQNGLVEVNVRVKEDFAIRGGLDDPTIQNMLMYAAR
                     HNQDAEKRLLALRLLNQIEPNPIVDATFVAAMLHDNNESVRLFAAQLLEKRQMTKQIQ
                     EAFQLVALRDSSASLRKIALENLVNKAEQEVVPVLALVAARDESEEIRQMALLALDKF
                     YDKNDREKR"
     gene            24563..25921
                     /locus_tag="PLI55_02395"
     CDS             24563..25921
                     /locus_tag="PLI55_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HPA99718.1"
                     /translation="MKPKKLFLILLMIGSVGASPDKFEFDGKQYVGNIVKTFAVGSKG
                     DLVMERVSGDVTIEGEARRDVMIVEKFKIDAYSEVNARQILNEEKARYQQRGNKVIVT
                     GKEHSRRYSSDFIVKVPLTFNTTVQTSGGDININKVSGLQNLNTSGGDIEISECSGEL
                     KSHTSGGDIKIQQVAGNLSVNTSGGDIEIELFDGELWANTSGGDINLDKIQGNGQVST
                     SGGDINIVNLRAKTFSAKTSGGDIGADKVEAELTLRTSGGDIIIGQIEGNLEIHTSGG
                     DIEVRQVNKNLKATTSGGNIDIGLIKGYCYARTSGGNIEVDRAYDLEAITSGGDILAK
                     GVVGYIYARTSGGDIEARKLFNSDVKNNAIDLETSGGEIEVILPANLNADIDAEITVT
                     SRRYADVNIHSDFPVQKSHEIKSSRIYIYARGKINQGGSLVRLRTINGNINILKADRE
                     SR"
     gene            25932..26804
                     /locus_tag="PLI55_02400"
     CDS             25932..26804
                     /locus_tag="PLI55_02400"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HPA99719.1"
                     /translation="MKRKLIILLIFMIAVLSAEDYFTEHYDIPYKGEEELDVNISFGL
                     GTLDLKSHDDPKYLVHSIMTYSQRIYKPIVDYKILGNRGRLKMSTEKYDESNKNDKRN
                     LKSSQVNKELNKWHLEFSQKIPIYFAVDLGLGEGRLDFTDLRVRDLKLHCGLSDVNVI
                     FRDKNQDNMRNMVIETGLGSVNVKGLSYANMERFNVECGLGTTELVFDGSLSQDIRGK
                     IEVGLGSVIIEVPDNVAVEVRSQSSFLSSLNLHGFDRISENLYRSGNWETANKRIYLE
                     IEVGLGSVDINWLD"
     gene            complement(26911..27276)
                     /locus_tag="PLI55_02405"
     CDS             complement(26911..27276)
                     /locus_tag="PLI55_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HPA99720.1"
                     /translation="MHSEHEIILIGDRVLIDPDEDQNKTPSGLFLPPGVKEKEKVQGG
                     FIVKTGPGFPIPHTDDIEDEPWEEGEDEPHYLPLQAQEGDYAIFLRKSAIEIELESKR
                     YLIVSHSNILLLIRENYLE"
     gene            27407..28342
                     /gene="trxB"
                     /locus_tag="PLI55_02410"
     CDS             27407..28342
                     /gene="trxB"
                     /locus_tag="PLI55_02410"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPA99721.1"
                     /translation="MAKDVVYDVVIIGGGPAGLTAAIYTAREMLSTLLLEKSMTGGIP
                     AIVDRIENYPGFPDGISGMELMQNIRAQADRFGTNISEFEDVSKIIPGRQCFEVISDN
                     RRFLAKAVIIATGGVPRNLGIASEQEFLGRGVSYCATCDGPLYRNAELIVVGGGNAAI
                     QEALYLTKFARRVTVIHRRDKLRAQPVLQERAFKNEKIRFIWDSVVSNIFGAEQVEGV
                     EIRNVKSGEKISLPAEGVFIFIGWVPNTEFVRGLVNLDDDGHIITDAEMHTNVPGIIA
                     VGDVRSKPFRQIANAVGDGAVGALSASKYLEGLHN"
     gene            28403..31255
                     /locus_tag="PLI55_02415"
     CDS             28403..31255
                     /locus_tag="PLI55_02415"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPA99722.1"
                     /translation="MKVHIPKSIVVAALFTTMIACSTSELTRKIPPTPPSPSQSLGLD
                     NEIALDPKITKGKFDNGLTYYIRKNEKPERRAELRLVVNAGSVLEEEDQRGLAHFLEH
                     MAFNGTAHFKKQEIINFMELAGMPFGSHLNAYTSFDETVYMLTVPTDRDSLLEKGFQI
                     LADWASQISLEDEEIDKERGVIREEWRLGRGAEMRIQEKEFQVIFWGSLYAERHPIGQ
                     IAVIDTFHYDTLRKFYRDWYRPDLMAVIAVGDFDEDRVKNLIEQNFIGLKSPADAPPR
                     PIPPVPDHTETLFSIQTDPELTRNQVDLLIKLPRTEQKYIRDYRQSIVENLYNSMLNE
                     RLEELTKTAEPPFLGAASQKGRLVRSKDCYSIGAVVKDNGIEEGFTAILTELKRVREF
                     GFTETELKRAKSTALRNMEQIYRERDKIYSRSFASEYIRNFLDNEPVPGIELEYEFYK
                     KYVPEIALPEVNVLAKEWVTDINRVVLVSAPEKPDLKIPTETELREIFEKVERQEVTA
                     YIDEVAESDLVRQLPPPGRILQETLIDSLDLTELILSNGVKVIIKPTDFKNDEILLSA
                     YSPGGTSLVPDSNYVAAISSVPIVTESGLGEFNSVMLSKKLAGKVVSVQPSLAELTEN
                     FSASASPEDLETMFQLIYLYFTAPRKDSTAYLSYLEKLKNSIINRAADPFAAFSDTIR
                     VTLSQYHFRARPWTIAMLDEIDLNKSYNIFRDRFGEAGDFTFFLIGNVELDSAKQMAM
                     RYLANLPTNNREENWRDVGIRPPNGVVQKAVYKGLEKQSYVQITFSGNFDWSRQNRFE
                     LATVTDVLRIKLREQIREEKGGTYGIYVGSSSKQYPYPEFRLTIQFGCDPERVTELTS
                     VIFQEIENLKSEPLGDEDLAKIRQIDLRSYETNLKENRFWLDNLHFYYFNNENPMAIL
                     HYPQLVEDLTKEMVQNAARRYLPLDNYVEVVLYPEQ"
     gene            31317..31841
                     /locus_tag="PLI55_02420"
     CDS             31317..31841
                     /locus_tag="PLI55_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPA99723.1"
                     /translation="MTNLELNILGIVSEGATHAYNIEKIIAERGIRERTNVGFSTIYA
                     VLNKMQKIGLLMSNLEPQKGLPSRRIYSITPKGRQIFNEEIFKALSQPQHQPSLFETG
                     LTYSGVLNKEALREALTLYDTELGRQIQNKVHELTALSNTNDQVQRALLTRPLVILQA
                     ERKWIRELMGLLLT"
     gene            31858..32163
                     /locus_tag="PLI55_02425"
     CDS             31858..32163
                     /locus_tag="PLI55_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99724.1"
                     /translation="MDVWISYLTSHPIIGVALTAALVLFIIMVIARLLKWAVISFIIL
                     ALAVGLTYKIAQPKKIIDRIQKDIESVTENDSEKVTDKLQKSTEEAIEKVKESFKKE"
BASE COUNT         9811 a         6411 c         6927 g         8916 t
ORIGIN      
        1 ggcagccgaa aaatctttct tctgggatat acatttatca gctttccatt gaatcagaag
       61 atttcaaaag aacattgaag aaaaaattgg taattatgga atgaatatgg agtgcagtga
      121 ccgtgttgat gcatgcagtg accccgccac gcttcgctgc ggggtcactg cactccaaag
      181 ctatattcca aagtaaaaaa atggagaatg aatataaaat atatgagcac aacccaccac
      241 atctgttttg tcctgggtcc aaatatatca taacgggtgc tacctacgga aagaaaccct
      301 ggctgaaatc agatgaggca aaggaacggc ttctaaattc tataaaaaaa ggatttgacc
      361 actatcattg gacgcttgag gattgggtaa ttcttgacaa tcattaccat ttaattggta
      421 acgcaccgga ggatgcctca acgctttctt taatgataaa cgatatatat agattcatcg
      481 ctttatggat taagaagaat gttcctgaat ctaaaaatga taaaatcgcc atgtataatt
      541 attgggacac ctgcattact tatgagaagt catatttggc gagattgaat tatatctggt
      601 ataatcctat gaaacacgga tatgttgaag ataccagtga ttggaaattt ggtagttatt
      661 acgaaaggat aaaagtcgaa aaggaagaga tggaagagtt acgaaaaaag tataaatggg
      721 ataaagttaa ggttaaagat gatttttaat atatggagcc cctatggagt gatatggagt
      781 gcatcaaccg tgttgatgca tgcaccgaca tggtcggtgc actccaaagg ggcactccat
      841 agttactcca aagctaaacc ccacatttta tttctaacat tgataataag gtcaaaaaag
      901 ttaaattagg cgggttttta attaacaatg aggaaatatg aaaagaactt atcagcccag
      961 caaccgtaaa agaaaaaacg atcacggttt tagaaaaagg atgagcacca atagtggcag
     1021 aaaagtcatc gccagaagac gagctaaagg acgtaaggtc ctatcggcct aatttgcccc
     1081 gtcgtgaacg gctcgccaaa tgggatcaac gtgcccaaat cattcataat ggaaaaccgc
     1141 taaaatatga ttgcctttac atttccatct taccccaaga tgattggaaa ttatcaatcg
     1201 tgataaaaaa agaatgcggg aatgccgttc aaaggaattt atacaaacgt aaaatcaggg
     1261 aggctttccg tctctgcaaa ccctattgta aacgaccggc ggcaatcgcc gttactgttt
     1321 ttaaaaaacc tgataatttg caggtaaaca cattatcaga aattctgata aacaacctaa
     1381 atttataaac ggaatgaaaa tatttaacat attgattatt ttaattataa aaacatatcg
     1441 cttgctcctc tcgcctcttt ttcccaacac ttgccgtttt acgccaactt gttctcaata
     1501 tgctttagaa gcctttaaga aattcaactt ttttaaggct tcttggcttt cgctcaccag
     1561 aatacttaaa tgccatcctt atcatacagg aggttatgat cccctcccaa ctccaaagga
     1621 ataaaaatgg atagaaaatc actaatcggc tttgttctca tatttgttat aattttgaca
     1681 atgccctact attttaaaat aatttcgaaa aattcacccg aggtcactca agagaaagtc
     1741 atagaaaaaa caccagaaag tgttatttct gaaacgattc cagcgcaatt atccgaatcc
     1801 catccctcaa gcgaagtgca acgcccggct tctttaggaa gtgaaaaacc tgacacgctc
     1861 acaatcgaaa atgatcttta tcgtcttaaa atatcctcgc gtggaggcgg cactatcacc
     1921 agcgtcgtct taaaaaacta caataaaatt atcggacgcg atacaagtct ggtcgaaatg
     1981 atcccgcctt ttgaggcaag gcggcttttt attaaataca taaaaagtaa tggggattcg
     2041 gtgaccattg atcaaaattt tagaattttt accgaacatc aagaaaccag taaaaactat
     2101 tataaaattt ccgatggcga tagtattatt attcagtttc aattgccaga gggcgataat
     2161 attatcgttt cgagaacctt tgtcttttac ggtgataaat acattataaa ctcgtacaat
     2221 gatctcggtg gaatcctcga tgaaatggct tctgataaat atgatctggt ctgggaaggc
     2281 ggaatcgcct atactgaagc tatgatcaaa gaagacgtct cctattcacg ggcttccgcc
     2341 tgcgggggtg gcgaaaccga aaatctcgat gtaaaaccag gtaaaactgg atcagctagc
     2401 ttggtgggtg caacctattg gacagctata aaaaccaagt attttgtcgc cgccctgata
     2461 cctcatcagc ccgctgaagg ttatcgccta actggcttag gggttccatt acaaggcaaa
     2521 gacttccaaa aaatattcaa tatgtatctg actttgaacg cttcctcaga aaatgcggta
     2581 aagatttatc tgggaccact tgactatagc ataattaaag gcttggctcc taatttcgaa
     2641 cacttgatga actttgggtt tagtttaatt aggcctatca gtaaaggaat cttatggctg
     2701 ttcaccaaga tgcatctttt tattcctaac tatggctggg tattaatcat tttttctgtt
     2761 ttgttaaaaa tcgtcctcac ccctttaaca aacagttcca ctcgctcaat gaaagaaatg
     2821 caaaatattc aaccgcagtt aaacgctctt caggaaaaat acgcttccga tcctcaacgc
     2881 cttaataatg aacgccttaa gttgatgcgc gagcatggtg tcaatcccat gggaggctgt
     2941 ttgccacttc tgcttcagat gccgatccta tgggcgcttt tcatagtttt tcgcaccacg
     3001 atagaattgc ggcaggcacc ttttattctt tggatcaccg atttatccgc accggacaca
     3061 attttcacct taccattttc aattccaatt tatggtagtc aggtaaatgt tttgcctttt
     3121 attatggcaa tttcgcagct ggttcagcaa aaaatatccg gtgcttctgg cggcaaacag
     3181 cagaaagtaa tgatgtactt tatgactatc ttctttttct ttttatttaa tcagtttccg
     3241 tccggtttaa atttgtacta tactctttat aatattcttt cgattattca gcaaaaatat
     3301 tttccaccca aaccaaaacc caagaaaccc cataaaacca attctcaaag actacgccaa
     3361 taccaaacaa ggatgcgcgg tcgctgaatg aattctactg agaataatgt caaccgaaaa
     3421 tacaatagtt gccctttcaa ctccccccgg gcgtggagcg ctggcagtgg tacgtctttc
     3481 cggtccaaaa tcaccggata ttgttcataa attaaccaaa accaccaccc aactaaaacc
     3541 acgagttgct cagcatctca atttatacaa aaacggcgag ttgcttgatg atgctgtgat
     3601 aatctattat aaagccccaa tgtcctatac cggcgaagat ttagtcgaaa tcagctgtca
     3661 cggaagccct ttcattgtca accaactgat tgaattatgc ctcaggtatg gtgccagatt
     3721 ggctcaaccc ggagaattca cccgacgcgc gtttctcaac ggtaagttcg atctgactca
     3781 agccgaagcc gtagccgatt tagttttctc tcaaacccaa gcgtctcacc aagccgcaat
     3841 taaatcactg gaagggacaa tcggaaagga attcgctgaa cttcggagta aaatcatcga
     3901 gctaataact attcttgaat tagaattaga cttttcagat catgaaatag atacgattcc
     3961 accgcacgaa ttgtcgaaat caattgataa catattgaaa attaacagca aactactaaa
     4021 atcctattct tgcggtaaaa tactaaagca aggcgccttg gtgccaatta ttgggccgcc
     4081 taatagcgga aagtcgagct tgttaaatgc ctttttaaac gaagaacgcg caatcgttac
     4141 gccttatccg ggaacgacta gagattcact cgaagaagca atccaagccg gtggctatat
     4201 gattagacta cttgacacgg ccggtatccg caaaaccaaa aacccaatag aaaaaatagg
     4261 cattgaccgc actaagaaaa atatcaagac aggcgatatt atcttattcg ttattgatgc
     4321 cactcaaccg attaaaagag aatggttaga atattacaat tctaaaaata ctatcgttgt
     4381 gatcaataaa attgatatcg caccacaaaa aagcctgaca aagtatgaaa agcatttctc
     4441 tcaatatccc catatatttg tatcggcaaa gaaacgccag ggaatacaca aattgaccga
     4501 gatgattgtt gattcaatct cgcaacaaat tggcaccgcc gacagcctca tcatcactaa
     4561 tcagcgccat gctaccggtc tgaggacggc aaaaaaatat ttactttctg cccagaaaca
     4621 cattaggcaa agtctagctc cggagatgat catcgccgat ctacggatag cgctcaacga
     4681 atatgacaaa attctgggaa aaacctctaa tgatgaaata ttaaacaata ttttccagca
     4741 tttttgcatc ggcaaatagt agtttcacat gaaacattta gactttgaca tcacggttgt
     4801 gggaggcgga cacgccggaa tagaagcggc gttggcggca gctcgtttgg gactttcaac
     4861 tttactggtt acccaaaata tttctaccat tggtagaatg tcgtgtaatc catcgatcgg
     4921 aggtttagca aaaggtcacc tcgtcgctga aattgatgct ctcggtggcg caatggcaaa
     4981 attgattgac cggactggaa tccaattcaa aatgctaaac aagtctaagg gaagagcggt
     5041 ttggtcgccg cgcgctcaag ccgataaaat tcactactcg ttgttggcga ggaacacttt
     5101 atcaaatcaa gaccggctca cccttctcga agatactgcc agtggcttga tagttgataa
     5161 gcagattatt caaggtattc atttaattcg taacggcgac atattttgcc aggccgtaat
     5221 tttaacttgc ggcactttcc tcaatggatt aatccacgtc ggactacaac aattccatgg
     5281 cggtcgaatt gatgaagaac cggtctccgg tcttacagaa gaccttgcca gatataatat
     5341 ctactctgga cgtctgaaga ccggcacgcc acccagagtc ttggccgaat ctatagattt
     5401 ttctaggaca atgccccaat atggcgatca agatccgatt ccgttttctt ttagtaccca
     5461 aaatttctcg ccacctaata ttccttgttt tttaacccgc acaaacaccg ttacccacaa
     5521 tttcattttg aacaatctgg atcgttcacc gctttattct ggcataatta aaggaattgg
     5581 tccgcgttat tgtcccagca tagaggacaa gtgtgttcgc tttaaagagc gcacatctca
     5641 ccaaatattt ttagaaccag agtggaaaaa tagcaacgaa tattacgtaa acggtttttc
     5701 gaccagcctg ccacttgacg ttcaaatgaa ggcattaaga acagtgccgg gtctcgaaaa
     5761 agcggagttt atcaagccgg gctatgccgt tgagtacgac tttttccctt cttaccaaat
     5821 taaagatact ctcgaaacca agtcactcaa cggactttat atagcgggcc aaattaatgg
     5881 cacttccggc tacgaagaag cggccgctct gggattaatg gccggtatca acgccgccct
     5941 ttccatccaa ggtcgcgaac catttgtttt gcaaaggcac gaagcctata tcggggttct
     6001 cattgatgat ttaattacca aaagtcccaa tgagccctac cgaatgttta cctcgagcgc
     6061 cgagcaccgc ttgatgctac gttttgataa tgccgatcgc cgacttgccc gaaaaggata
     6121 tgaactcggt ctgttgccgc gagagactta cgaatcaacc caacataaga tagaagttat
     6181 tgaggagatt atatcctttt tatcttcaac aaatatttct cccgaagcgg ctaaagtcat
     6241 attacctaat aatgacgccc attccattgg ctgtaatctc tctttatata aattgctttg
     6301 ccgtccggat gttgcgctcg cttcgctcgt tcctttttta tcatctgaaa taaaaaataa
     6361 gattgctcac tttcccaaga ttattgagga gattgagtgt gacgtcaaat atgctggtta
     6421 tattaaacac agtcaagcct taatcgaatc aatgtcaaaa aatgaaaata ttaagattcc
     6481 ccctgatttc gattattctg cggttcgatc tttaactaac gaggcgcgtg aaaagctgat
     6541 tcgcttccgc ccgcaaacac ttggacaggc ttctcgaatt ccgggtgtct ctccagcaga
     6601 cgtcgcggcg ctgtttttcc tccttcataa gcattctggt ttaacgtgaa acatttagac
     6661 gaccgtatag aaaatttagt ctctaccttt tccgaaagtt cagcttttct taatgccgtt
     6721 aaggcttttt atcgctctaa cgctgggttt accgttgcgg gattatccgg ttttgcaact
     6781 gcgctttttc tctattcttt ttataaaaaa attgaccagc caattttatt aattaccaat
     6841 gattccaatg aagcggaata cataagagat gatttggagc tgtttaatct cgacgactcg
     6901 gtatgctatc tgccggagcc gccgaattct tccttcctat tttctgatac ggaatcgctc
     6961 aatgcttact tcactaacga tacccttcaa aaactatcca ccggacaaaa tacgatcatt
     7021 gtagctactt tatcttcgct taaaagtaaa ttcccgcttt ctgatgattt caataagctt
     7081 aaacgcacct atcacgtcaa cgaaatagtc aatcgcgacg cttttatcga atatcttgtt
     7141 catctcggtt ataccatagc accagcagtg gaaagtccat ttgaatactg catcaagggt
     7201 ggaattattg atttttatcc tccagaatct ctgcatccct atcgaataga atttttcggg
     7261 gataagatcg aatctattcg cacttttaat cctgacaccc agctctcgat ttgcaatcta
     7321 tcatccttca cagtacaagc tccggcgcat tcatcgctag ataatcaaaa ctctactaat
     7381 atcttttctt accttaaaaa agacaccgtg gttttttgcc ttcatgctga aaaacttatg
     7441 gatacagatg aaaaaaacac gactgatata tttacttatc tatcatcata cagggtaatt
     7501 aatcattacg acttacttgc ttcagatgtt gatttccggg tgaatcctca accgcaaacc
     7561 aattcaatta aagcttttag agaacattta ttgacagtgc tctctgccca gccagatgcc
     7621 cgaatctcgt tgttttgtgc taattctatg caggtggaac gtcttaagga attactgggt
     7681 ttggaaaaat tgaactactt tgatttttct atctcggcgg gggcagaact acccgaagca
     7741 aagttgtatt tttataccga tcaccaaatt ttcaagcgcg aacgacggat aaattttttc
     7801 aaaaagtatt ccccagactt tcctgttgaa caattcagtc cgcacgaaat atctcccggc
     7861 gatttgatgg ttcatattaa ttatggggtt ggccgctttg ctggattgga taaaatcgcg
     7921 gcttttggtt cgatccgtga atgtctttgc ctggaatatt ctggcggaga taaagtattt
     7981 gtaccacttg agaagcttaa ctatgttcag aaatataccg gaaatcttca gaatccgcca
     8041 cctctcaaca aacttggtac gacggaatgg gaacgcacca aattaaaaac acaacgagct
     8101 attgatgaga tttcgaaaga tctagtcgaa ctttacgcca aaaggataag cgcgcccggc
     8161 tatgcttttc caccagacaa tgaactgcaa tacttaatgg aatcggaatt tttatatgaa
     8221 gaaactcccg accagctgac tgctattcaa gccgttaaag aggatatgga aaaacctcgt
     8281 ccaatggatc ggcttttgtg tggcgacgtg ggttttggaa aaaccgaagt agccatccgg
     8341 gcttctttca aggcggtatc cgcgtccaag caggtagcgg tattggtacc aaccactatt
     8401 ctggctgatc aacactttgc tgtttttagc cagcggcttc aaaaatatcc gataagaatt
     8461 gcacttttat cgcgattcgt atcccgcaaa atgcagtcac agattattcg tgatcttgcc
     8521 gcgggaaaga ttgatattgt cattggtact caccgactac tgtcaaaaga tgttagtttt
     8581 aaagatcttg gtttattagt tatagatgaa gaacatcgct ttggcgtcaa agacaaagaa
     8641 cgaattaaat ttttacgcga atcggtagat attctggctt tatcggctac cccgatcccg
     8701 cgttctttaa atttttcttt gattggagta cgcgactttt ctgttatcaa caccccgcca
     8761 atctctcgct tgccagtaat aaccgaagtc atcactttcg atatgaatat tattaaaaag
     8821 gctatcttgc gggagattaa tcgtggtggt caggtctttt ttgtgcataa cgacatcaaa
     8881 tctattgccg ctctcagtca taaacttgct ctggctctac cagaaatcag cgtagattat
     8941 gtccatggcc aaatgaatga aagagaactc gaaaagacta tgtcggcttt cattaacaat
     9001 gaaatcaatt tactggttac cactacaatt atagaatcag gaattgacat tcccaacgct
     9061 aatacaattt ttattaatca agcccatcat tttggtctgg ctcaattata tcaattacgc
     9121 ggtcgggttg gcaggagtag tcgtcgggct tacgcttact tgattgttcc cgaaccgtct
     9181 cgcattaaac cggacgccct tagaaaactc cagactatta aaaaacatac cgcactcggt
     9241 tccggttata gtgtggcgct tcaagatctc gaaatacgcg gtgcgggaaa ccttctgggt
     9301 acctcgcaaa gcggcaataa tatagcggcg atcgggtatg agctctatat gaaatttttg
     9361 cgcgaggcgc ttgatgagat caaatcaacc cagcccgctt acccagaaaa attaaaattg
     9421 gaaactgaag ttttttgccc acttccagcc tattttcctg acagttatat ttccgattct
     9481 tcggcacgtt tagaatatta tcgccaacta acactggcgg atgaattgtc tacagtagat
     9541 ttaatctcag gacaaatccg cgatatttat ggaaagttac cgtctgaagc aacgactctt
     9601 tttaatatca gcaagattcg gctcctcgct tcatcacttg gaataaaaaa agtcaatatt
     9661 gccgaaaaat caaccagctt tatctgggac gaatctttta taccgccagc cgaaaccgat
     9721 ctcatccaag ccatccaaaa ggccgcttct gatttgggtt tgtcttataa gtttaatcct
     9781 tacaatacct tatatttaac cgtttattta aaaagtaatt ttgattttga taaaattact
     9841 cgcttcttga atctgcttcg taacaccctt aacttatagt atctttttag gagtgtaaaa
     9901 agttatgaaa atgtattttt attctaaaaa tgtaggtttt gggctagaac taaatctccg
     9961 tttgattgtt aaagcataga ttttagtttg tttatgatag gtaacttagc ttgggaggta
    10021 taaaattgcg caaggacatt ttattaatga tattcatttt tgtctgggcg gcttgtggca
    10081 atcataaaga agattatatc gcgcgcgtcg gaaatactta tttaaccaat gaagacttaa
    10141 cccgaatgtt acccgacggt ggcttatcaa aagatattga taaaaattac ttaaactctg
    10201 ttgtttccaa ctggataaac aaggagatac tttatcaaaa agctcgccag tatcattttg
    10261 accgcgaaga gggcgtacaa gcccgggtag cggattatta tcgtgattta actatagatt
    10321 cctatgtacg ttttttctta caaactaatg taacgattag tgaagacgaa atacgtgatt
    10381 attaccttaa aaataaaaaa agttttattc gcgatcgtga ggaagctaag atcgttcatg
    10441 ttcttgtgca ggattttaac gatgcgctgg caataaaatc tgctataact agtcgcaata
    10501 agaatgcgct tgatgcgttt tatagtaagt ataaatttga gacctctgtc gttaagcgag
    10561 gcgaatcttt ggaagaaata gatcgtaata tttttgaaac ttctcctcgt tcgatcattg
    10621 gtcccattgc atcaaattat ggttttcata tcgttgaggt tctagcccgc tatcctatcg
    10681 gctcaacgcg aactatcgac gaggttcgtg atgaaatcta tcagatattg actcagaaaa
    10741 aaatccaaga tcagtataac gcactggttg acagtcttgt ccggaacgct aattatgaaa
    10801 taaaagatga aaatatttcc aattttttaa actctatagg ctctcgatga aaattaagtt
    10861 gttttttggt attatctttt ttgcgaccac tcttttcgct caaacgctca ttgacggtat
    10921 cgccgcaatt gtcggcgaga acattatcct cataagtgag gttgatcagg tggcgcgaat
    10981 cacggcttct cagatgaata ttgatcctaa ccgcgacgtt gcaaatttta ataaaatcaa
    11041 acgtcatgtt ttaaattctt taattgatga gaatgttatt ctggaacaag ccagaattga
    11101 aacggtggaa gttaaagagc gcgatgtgga aaatgcgatt aaccaacgtt tggatatgct
    11161 cgttgagcag gttggttcgg tagaaaatct cgaattgttg atgggcagtc ctatcagtaa
    11221 aataaaaaaa gattaccgtc cgatttttaa aaatcagctg atcgtcgaga aaatgcgttc
    11281 cgaaaaattt agtaaagtaa ccgtcacacg acctgaggtg gaggcttttt atcaaaccta
    11341 caaggacagt attcccccga ttcctccatc actggatttt agtgcaatat tattcaaagt
    11401 cactcctggt cccgaggaag aaatggcggc taaaaaatta attgattcgc tctatacttt
    11461 actaaaaaat gggtatgatt tcgagtatct ggctaaaaat tattcggagg atgaggcaag
    11521 tgctccttat ggaggagact tggggtatat taagcgtggc gcatttatta agagttttga
    11581 agaggtggct ttttcactct cgcccggtga gattagcgac gtaattaaaa ccgatttcgg
    11641 ctatcatatc atccaaatgg ttgaccgaaa aggtgaaagc attagggttc gtcatatctt
    11701 gatcaaaatc aaggtgtccg ataaaaacta tgaagaccga tatcgcttcg ctgatagtat
    11761 ccgtactatg attataaata atgaaatttc attcgattct gctgtagtaa aatatagcga
    11821 cgaacctaat gccaaatcca ccttagggcg tattagacgc attccgctaa atcagattca
    11881 aaatacagat tttattaatg tcttaagtaa tatgaaggtt ggagatatta gtccggtttt
    11941 tgagtcgtca cccggatatt atattttaaa acttaataac gtttatgatg atagttggcc
    12001 aacgatagaa agatttgcac tggaatttaa aaagaatcaa ctgtatcaag agtggctaaa
    12061 taagctaaaa gaagacatct ttattgacat caaggtaaat tattaacagg ttaacactat
    12121 tttaattatt tgttaacaag gtgcattagt ctttccttag atcacgacga caaatacttt
    12181 actcctgttt aggaacgtta acaaattatc aacagtgtat tttacaatta ataatcttct
    12241 caatcatcgt atttttaaat gttgttaaca aattaacatc tattatttaa ctactaatat
    12301 tttaataatt caaagttact taacatataa aaattacttt tgattatttg ttttaaatta
    12361 cttggaaaaa acagtattct ttgataaaat cttgttatga aaatattaaa agtaaggaga
    12421 tagaatatgc agttttctgt tagccaaaat ttactactta ataatctaaa taaggtagcc
    12481 aaagtgacac ccgccagaac aactatgccg atattgaata gtattctttt tatcttaaaa
    12541 gattctaatt taacactaag agcttccgat attgaaatta ccttaagtac gacttttcca
    12601 gtgaacgggc aagaagatgg aagtgttgca gttccatctc gactcataca agaaatagta
    12661 ggtgagatta gagagggtga actaaccttt aatgctaccg atgaaggcga gataaaaatt
    12721 actgcaggaa aaggtaaata taaaattatg ggaaggccag gtgaagaatt tccagctttg
    12781 ccaagtatta ttaatcaaaa tagtatttta gttaacagta aaattttatc gagaattatt
    12841 caaaaaacag tgtttgcggt tagtagagat gaacttaaac cgtctttgac cggcgtactt
    12901 tttgaagtca aagctgacga acttagatcg gtagcgacag atggtcaccg gctggtatat
    12961 attaatagaa aagatttcag ttcgccggat tatgaacgac agatcatagt cccggttaaa
    13021 tttctcaatc taattaatac ctaccttacc gatgataata aggttagttt aactataagc
    13081 gacaatcaca taatgatgga aagtgattca accctgataa tttccagaat aatagacgaa
    13141 cgttttccgg attatgaaag tgttataccc aaagataatg ataaggagct gaaagccgat
    13201 tcagattatc tttcagcggt tttgaaaaga gtgtctatat tctctaataa atcaacccat
    13261 caaatatcgc taagtttgaa tcgagaactg tctaaaataa gcacgataga tttagaaaca
    13321 atgtcgtcgg cagatgaaat cctcgatgtc gaatatagtg gcgacgatct tacaattgga
    13381 ttcaatgccg aatatcttcg agaaatcgtt aggaatattg atactcaaaa agttataatg
    13441 aaattaaaga ctccggtcag cgccagcctg gtaatcccgg agaatcaaga agaaaatgaa
    13501 gatttaataa tgcttttaat gccgatcagg ctaagtgatt aacacctctc tttattttcc
    13561 gtaaaagatg attattaatg gtattgaaaa atttgagact tgtaaatttt agaaaatata
    13621 ccgattccgt ttttgtattt cacgaaaaga caaattttat aaatggagat aatggtagtg
    13681 gaaaaacttc cctactcgaa gccatccatt atttatccct aaccaaaagt tttcgtacga
    13741 ataatgatag cgaagcggtt aattatcaaa gtgattactt caatattttt ggtgagtttt
    13801 taagcgataa caatcagaca attttgtcta atatgaatta ttctaaaact gaaggccgca
    13861 gttttttgat gaataaaaat agggtaaaaa gatacaccga cgtcgtcggt tgtgcgccag
    13921 tagtgatatt atcaccagga ttacagaaaa ttacggaagg cggcccagcc gaacgtcgta
    13981 atttcattaa tcggataatt tcgcaaaccg atcgcaatta cctggtaact ttgatagaat
    14041 atcgccgtag aatctcacaa agaaatgtcg tactcaacca gtatcgagag aaaaaacgct
    14101 gtcgttatga tgcttatttt gaagcccaag acgaaatctt agccagactg gggcaaaagt
    14161 tgtttattgg acgacaggca tttattgccg atatggactc gattttcaaa acagtctttg
    14221 cgcgaattac ccatattggc ggaacggtag gaattgatct gaaatataat attaaagccg
    14281 acccggacaa ttttatttct ttgtttacca aaaacttaag agaaaggttc agccgcgact
    14341 tacttatcgg gcgaacaacc tgtggacctc atttagatga aattattatc aattataacg
    14401 gcagagatat tcgtcagatc ggctctcagg gtgagcataa gttaattctt atagccttaa
    14461 aattggctga aggagcgtat attgagagcc accaaaacga aaaaattata tttttattag
    14521 atgatctctt tgccttttta gatggtaaac actgtcgtaa aatcgttgaa gaaatcggtg
    14581 ataaaaatca ggcgctgatt acttcaaccg acgtcaaaat gttgcgaaag tatggttttc
    14641 ttgataatcc tgaaaaatat aaaattttta atctgactac tggggaagca taaagatgga
    14701 gtctctcggt acggctattg ccagaatgct aaaaatttat ggcatagaaa agccggttcg
    14761 tcagaatgaa gtttttttta tctggcaaga cattgtgggt gaagtcattg ccaagcatgc
    14821 cataccagaa aaagttgcct ataataaatt atttgtcaaa gttgattcgc cggtttggcg
    14881 caatgaatta aactttcgca aggaagaaat attaaagcgg ataaacagtc aaataaaaaa
    14941 tgcacagatt aaggagatta tcttaaggtg agcgaaatta aatttaccga cgccgcaatt
    15001 gattacgggg ctgaaaaaat tacagttctt aaaggtttgg atgcagtccg aaagcgaccg
    15061 gctatgtaca ttggtgacac cggtaagcgc ggtttgcacc accttgtatt tgaggttatt
    15121 gataactcga ttgatgaggc attagccggt ttttgcaata agattgtggt aactgttcat
    15181 gaagataatt ccgttagcgt actggacaat gggcgcggca ttccgatcga tattcataaa
    15241 gaagaaaaga aatcagccct cgaggtcgtg atgaccatac tgcatgccgg aggtaaattt
    15301 gataaaggtt cctataaggt ttctggtgga cttcacggtg tcggcgtttc ggtggtcaac
    15361 gcgctttcag aatggatgag ggtcgaagta tatcgcgacg gcaagattta tactcagagt
    15421 tattcacgag gggtacccac cagtaaagtc gaaccagttg gcaagactaa taaacatggg
    15481 acgctcgtaa ggtttaaagc cgatgttgaa gtattcaaga aaatagagta tgaatgggaa
    15541 gtgctcgagc atcgttgtcg cgagcttgct tttctaaacc gggatttaga aatcgttttg
    15601 gttgatgagc gtgataaaaa cgagaataga ttccattata aaggcgggct agctgaattt
    15661 gtaaattttt tggatgaaac ccggccggct attatcgata aaccgataac cgcggaaaaa
    15721 gaagaggacg gcgttttcgt tgaatttgca atgcaataca acacttctta taatgagaat
    15781 atcttcactt ttgtaaataa tattaacaca attgaaggag gaactcattt agtaggtttt
    15841 aagacggctt tgactcgcac cctgaacaac tatgcccaga aaaacaatct tttcaaaaaa
    15901 gagtcatttc agttgtctgg cgatgacgta cgcgaaggtt tgacggctgt gattagcgtt
    15961 aaggtgcctg aaccccaatt cgaagggcaa accaagacta agctcggaaa ttcggaagtt
    16021 aagggcattg tggactcgat cgtctccgaa agtttggcgg aatatctgga acgtaatccc
    16081 actcaggggc gtcgtattat cgagaaaagt cttaatgcgg ctcgttcccg tgaagccgcc
    16141 cgtaaagccc gcgaattgac gcgccgtaag agtgcgctgg atatcggcaa tcttcctgga
    16201 aaactggctg attgttccac cagagatcca tcaatgtgcg aattatttct ggtcgaaggt
    16261 gattccgcgg ggggtagtgc caaacagggg cgcgatcggc gctatcaagc tattctgccg
    16321 ttgcgaggca aaattatcaa cgttgaaaaa acccgtattg acagagtttt gagcaatgag
    16381 gaaatccgta ctattattac agctcttgga accggttttg gtgacgatga attcgatatc
    16441 caaaagttac gatataacaa agtcatcatt atgaccgatg ccgatgttga tggtagccac
    16501 atacgcaccc tgctgttgac ctttttcttc cgttatctgc ccaaactaat agaggatgga
    16561 catatctata tcgctcaacc accgctttac aaagttgtat gtaaaaagca agaatattac
    16621 gcttacgatg atgaagagct tgaagtcttg accaagagat tttcgaagga ttcgactgcc
    16681 aagatagata ttcagcgtta taagggactt ggtgaaatga atccagaaca gttgtggaac
    16741 acaacgatga atccggagac tcgcactctg ttaaagatta cgatagaaga agccgccgcc
    16801 gccgatcgta ccttctcgat tctaatgggc ggtgatgtcg aaccacgtcg gcaattcatt
    16861 caaaaaaatg caaaatatgt taagaacctg gatatttaaa aaatggcatc acaagtaaat
    16921 atcgaatttc aaagagataa aatcgtaccg gtcaatattg atgaggaaat gcgcagttcg
    16981 tacctcgatt attccatgtc ggtaatcgta gcacgcgcac tgccggatgt acgagatgga
    17041 cttaaaccgg tacaccgccg aattttatat ggtatgtcgg agttgggcct gacatataac
    17101 cgtccgacca aaaaatgcgc tcgtatagtc ggagaagtgc tcggtaaata tcacccgcat
    17161 ggcgatatgg cagtttacga tgcgctggtc cgaatggcac aacctttctc gatgcgttat
    17221 ccattagtag atggccaagg gaactttggc tcgttagacg gagacagcgc ggcggctatg
    17281 cgttataccg aagctcgaat gagtcgtatt gcgtccgaat tattgcggga cattgataaa
    17341 gaaacagtca aatttgtacc caattttgac gagtctcttg aagaaccaag tgttctgcca
    17401 acagccgtcc cagtaattct ggtcaatggt tcgagcggta ttgcggttgg tatggcgact
    17461 aatataccgc cgcataattt aacggaagtc tgcaacgccg ctatatatgt tattgatcat
    17521 ccggaagcaa cggtagacga actgatgcaa tttatcccag gtccggattt tccaacaggt
    17581 gcttttatcc tcggtaaaag tgaaattaag cgtgcttatg aaaccggcaa aggcgtaatt
    17641 aaaatgcgcg cccaggcttt cgttgagaat agcaagaccg gtaaggaaag catcatcatt
    17701 acggagattc cttatcaggt taataagtct aatttgatag aaaagattgc cgatctagtc
    17761 agagaaaaag tcatagacgg tataaccgat attcgggacg agtccgataa ggaaggcatc
    17821 cggatcgtca tcgaattacg ccgagacgct tatgccgaag ttatcctcaa ccagctttat
    17881 aaccacactc aattacaaga aagcttcgga ataaatatgc tggcgctggt caacggcgtt
    17941 ccgcggcagt tgtctctgcg agaaattcta caacacttta tcgattttag gcatgaaatt
    18001 atcataaagc ggacacaatt cgaccttagg caagccgagg acaaggcaca tatcctcgag
    18061 ggtttgaaaa ttgctctcga taatatcgat gaagtaatag ctattattaa agcatcaaag
    18121 tctcccgaag atgccaagca aaatcttatg atccgctttc gattttcgga acgacaggct
    18181 caggctattc tggatatgcg tttacaaaga ctcacgggct tggaagtcaa taaagtcatt
    18241 gaggaataca aagagactct caaacttatc gaaagactaa agttcattct tagcaatgtc
    18301 agtgaacaga tgttgattat taagaatgaa ctggcagaag tcaaagaaaa atatggggat
    18361 gcgcgtcgca ctcagatcat tgcggacgaa ggcgaatttt cggtcgagga tatgattgct
    18421 gaagaagata tggtgattac tattactcat aatggtttta ttaagcgctt cccggttgat
    18481 gcctcgcggc gccagaatcg cggcggacgc ggcagtctgg gagcgcaggc taaggacgat
    18541 gatttcgtgc gcaacctatt cattgctaat acccatgatt atcttttagt ttttaccaat
    18601 aagggacgct gttattggct gaaagttcat gaaatcccgc gagccggaaa aggtagccgc
    18661 ggccgcgcca ttgtcaattt gatcaatact cagcccggcg aagaagttcg ggcggtgctg
    18721 ggagtgaaag aattcgacga tcaacatttt gtcctaatgg taacccgcaa cggtatagtt
    18781 aaaaaaacgg tcttgtccga atttggcaat ccgcgtaaac ccggcattcg agccattaac
    18841 ctcaatgata acgatgatct gctcgatgtc cagatcacaa atggtaatca ggatattatt
    18901 ttagggacgg cttatggcaa gtcaatccgt ttccatgaaa gcgacgtaag gcctatggga
    18961 cgcaccgcgg ccggtgtacg aggcgttcgt ttgagcggca aagatgaccg ggttgtaggc
    19021 gttgtaattg ttcgtaatga aagcggcaca attttagccg tggctgaaaa cggttacggc
    19081 aaacggagcg acatcaaaga atatccggtc atcagacgag gtggcaaagg agtcataaca
    19141 ctcaagacct ccgttcgcaa cggtcggatg gtagccttgc gcgaggtggt tgataccgac
    19201 gatttgatga ttataaccgc caaaggcgta ttgattcgcc tgccagttgc tactctcaga
    19261 accattagcc gcaatactca gggcgttcgg ctaataaaat tggacgaagg tgacactatt
    19321 tcagccgtga ctcgcgtggt ggaatcagaa gataaaaaca acggcaatgg cgagttggaa
    19381 gaatccgacg aaattaccct gctcgaaaat gacgcgtctg acgaggttgc cgaaactgat
    19441 gaaccagagt aatctaatct aaaaggatta gccttcttgg actcgcagac cattcaaaat
    19501 aaccttttga taatcaaaga acgcatagtg caagcggctc aaaaaacagg gcggcgagcc
    19561 gaagacatcc agattgtcgc cgtaaccaaa acatttccag caacattggt tcgtcaggct
    19621 tataccgccg gtttacggat tttcggcgag aatcgcgttc aagaagccat tccgaaaata
    19681 atagccttgc aagattgccc ggaaatcagc tggcatatga ttggacacct tcagagcaat
    19741 aaagtccgga aggctatcga gaattttgct atgattcaat cggttgactc gcttgatttg
    19801 tgtaataaaa ttgccaacat tagccgtgca ctggggcgga aagtggacat tttactcgaa
    19861 gtaaatgttt ccggcgaagc cactaaatca ggtttacctc ccggaaaagt attttccgtt
    19921 gtggaacaag tggcggagat gccagaactc attttacacg gattaatgac gattggccca
    19981 ttaacgaacg accgggattg gactcggcga gcatttgcca atcttaaagg catttttgat
    20041 aaaattgctg agagtcggat caataccggt caattcaaac tcatttctat gggaatgacc
    20101 gacgattatg aaattgctat agaggagggc tcaaatatga ttcgcctcgg gcgggctatt
    20161 tttggggagc gcagaacatc atgatcaaga ctgatgaaat ccgcgaccgc cgattccgca
    20221 aagtattccg tggctatgat ccggttgagg ttaaatattt taccgaaatg ttggcggatg
    20281 aatttgctca gatggaagaa aagctagaga aattggaagc catagtaaat gagtttgacc
    20341 agactcgcaa aggacgcgat aaaattttgc aagaggctca agatcgggcg gcaagtatta
    20401 tcttggatgc tgagtccatt gcttctgaag taattgccaa tgcagaaacc aagaagaggc
    20461 aaatacagga ggcaattgat cagctaaacc acgaacagaa taaattgata caacgattac
    20521 gaagtgtctt gaaaaatcag gcggaaatac ttacctggct cgaggaagca ccagattggt
    20581 cggaagaaga taacaccctt aacgagtcat ctgaggaaac aacctgaaaa attatgtccc
    20641 taaataaaat ttataacgca cttataatga ataaagccgt atgcccgcaa ataccagagg
    20701 gtgagttaaa ctttaaaata attcttctcg ataatggggc aaatgttaaa acctggcgtg
    20761 aagccgttcg ctcggcgatt aaagatggcg ctgaacgttt tttattggtt gggcgagggg
    20821 tgccaacgcg gcggtccgaa aagtccgcag gaatagtagt tgtagatcag attaatgttt
    20881 ccgggcagaa tcccttggtt ggtcctaatg atgaagaatt cgggacgcgt tttccagata
    20941 tgaccgattt gtacagcccg aaattaactg aggatgtcct tatcgctggt aaacaagccg
    21001 gtctcgtttt acagaaaggt attctgcttg tacctgtttc aatgaccagt tcaacatctc
    21061 tggaagaaaa attgatagta caaaacgaaa tcgccgcact ttctcaggat gttttcgccg
    21121 gagcgattac agtcagacat gccggacgtc aatgcactgg aataatttta ttttcagata
    21181 ttgatggtaa acaattgggt ttgttacttg ctcacctgga ttggtagctg attttaacgt
    21241 ctccactgcc ttcctcaaga ataacaattt tttattttct cgtattttaa tagattgcct
    21301 gctgaaatga ttcagcgcat ccggaacata tacgttcatc gttttcgggt gtttgggttt
    21361 ctactattaa tccttcctaa cattgtatgg gctggacatc cggttacaat agataaattc
    21421 accaatcaac cgactatacg agcctggatt tatttatcga acgaaccagc ttataatgaa
    21481 tattttacgc aactcgaata tccggaaaaa actcaacgac gccttgccaa aatgggaacg
    21541 ttagccaaga agaccgcttc tgaccttgtt cccggtgaag aattacgggc tattattgnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt acgaatcggt tttatagata
    21721 ccggattttt taaagaccat gaggttttta gtgaaatttt atcgtccggc cgcttgatag
    21781 ccgaatatga ttttatcaat gatgatgaca acgtcagcga tgaagaagcc gcggataccg
    21841 cccgaaacag ccggcagagt gaacatggaa ccgccgtctg gagcttagtc ggcggatata
    21901 atccacctga ttatcttgga gcggcttatg gcgctgaatt tttactagcc aaaaccgaaa
    21961 gaattgatac cgaaattcat cgtgaggaag atgattttgt cgccgcggtg gagtggtgtg
    22021 attattgggg ggctgacatc gtatcggtat ctctcgggta tcgtgatggt tttgcagatg
    22081 gttctaaata tccttatgaa taccttgatg gccaaaccgc cgtcaccact aaggctgtta
    22141 actgggcgtt cgagcggggc attctctgcg tctgttgtgc tggtaacgac gctaataatt
    22201 ttgaggatgg cggactgctc acacccggcg atgcttttgg agcattgacg gtcggtgcgg
    22261 ttgatgcgca gggtgaaata gcctccttca gttctcacgg tccaacctac gacggccgaa
    22321 tcaaaccgga tttatgtgct atgggcgttg cgacttatta cgccagtaat tcgcaaggag
    22381 cttatcgtac agggagcgga acttccttgg cgacaccgtt gattgctggt gccgcggcgc
    22441 tattgttgga gagtcagcca gagctgaccc ccgcagaaat tatcacggaa ctgaaacgtt
    22501 atgccgatcg cacccaaaac ccagataata gatacggctg gggcattcca gatgtctatc
    22561 aaagtatcgt caattaccaa cccaagagtt tcccgaaagg cgaactatct ttcgatgaga
    22621 tttgggttta ccccaacccg gctaatagca acataaattt ttattttatc tggcataaag
    22681 ccacgcccgc taccgctccg gcttacctac aagtcataaa tctcaacgga caaatcgttt
    22741 ttgaaaaaag gctggttagc aaatttggcg gccaaaaaga agtaataaat tggagtttac
    22801 ataatcaatc tggcaatcgt gtcagctctg gaatctattt tgtatgcctg caaaatccgg
    22861 agtattttaa aataagcaaa tttattatca tgcgctaatc ttagtttgac aacctttatc
    22921 gccgacgtga atctaaagcg ggtagatgta tggaaaaaga agaagtcgaa ttaatccaga
    22981 atattcaaag tggcgataaa atggcattcg cacgtttaat ggaacaatac ggtgagcggg
    23041 tgatgagtat agcctttcgc tttactcgca actatcagga tgccgaagac ctttatcaag
    23101 agacctttat taaagtctat cagaatctgg gaaaatttcg cttcgagagt gaatttttta
    23161 cctggattta tcgcatacta gctaatcaag cttttaatct atataataaa cgcaaacgta
    23221 tgactttagc tgatcccggt gaagacgact acctgtggga gaccattcct gccgacgctc
    23281 aagacgaagc cgatcgggta acattcagca attccattca ggaccaaata gacgccgctc
    23341 tccagcaact atctgcaaaa caaagagtag tattcattct taagcatttt gaagggaaaa
    23401 aaataaagga cattgcgatt ttgctcgatt gcaccgaggg aaccgtcaag cgctatttat
    23461 tccgcgcaac tcacaaatta caaacgcttt taactgaatc ataagaaagg atttagatat
    23521 gaagcacgat aaacatttta aagaactttc aatcctatac ttttacaatg aactatcgga
    23581 atccgaacga actgatttta aacatcatct ggacgagtgc caagtctgcc aggccgaaat
    23641 agaacgtcta caaattattg ccaagaaaat agactacgct actgaaccgg tgcccagcgc
    23701 ccgacgattg gaagcattga acaagcaagt aatatcaaaa atttcatccg aatcgcgccg
    23761 accgaaattg gtaaatgtca aaacatattt tgaggaaatg tttgagacga ttacaatcgg
    23821 cttgtcccgt ccgcgttatc aactaattgc cgccagttta attctagtaa tcggcatttt
    23881 tattggaaga gtaggattta gagccgactt acccaaacaa ccggaaatac ttgccaactt
    23941 tgttaattac aactataaat tcaccgatcc ggagaaagaa aatattcaaa aagcctttac
    24001 taactattta ctaaaatcag gggggataga agatgctgac ttacttcaaa gtgaatatcg
    24061 aaccagccag aatggattag tagaagttaa tgtcagagta aaggaagatt ttgccattag
    24121 aggtggatta gatgatccca ccattcaaaa catgttaatg tatgccgccc gtcataatca
    24181 agatgccgaa aaacgccttt tggcactgcg cctattgaac cagatcgaac ccaaccctat
    24241 cgttgacgca acttttgtgg cggctatgct ccatgataat aatgaatcgg tacgcttatt
    24301 tgccgcccaa ttgttggaga aacgacagat gaccaagcaa attcaggaag cattccaatt
    24361 agttgccctg cgagattcaa gcgcatctct tcgtaaaata gcgctggaaa atttggttaa
    24421 taaggcagag caggaagttg tgccagtatt agctttggtg gcggcgcgtg atgaatctga
    24481 agaaataaga cagatggctc ttttggcttt agacaaattt tatgataaaa acgatcggga
    24541 aaaaagatag agagcatatt cgatgaaacc gaaaaaatta ttcctaattc tattgatgat
    24601 tggctcagtc ggcgccagcc ccgacaaatt tgaatttgat ggcaagcaat atgtcggaaa
    24661 catcgttaaa acttttgcag tcggtagtaa gggcgatctt gttatggagc gtgtcagcgg
    24721 ggatgtcacg atcgagggtg aggctcgccg ggacgtgatg atcgtcgaga agtttaaaat
    24781 agacgcctat tctgaggtta atgcgcgcca aattttaaat gaggaaaaag cacgctatca
    24841 acaacgaggc aataaagtaa tagtcactgg caaggaacat tcgcgccgct actccagcga
    24901 ttttatagtg aaagtgccat tgacttttaa tacgacagtt cagacttcgg gtggcgacat
    24961 taatattaat aaggttagtg gacttcagaa tctaaatacc agtggcggcg acattgagat
    25021 tagcgaatgc agtggtgaat tgaaaagcca tacctcgggt ggtgatatta aaattcaaca
    25081 agttgcaggt aatttatccg tcaacacttc tggcggtgat atagagatcg aattatttga
    25141 tggcgaactc tgggctaaca cttccggtgg cgatataaac cttgataaaa tccagggtaa
    25201 tggccaggtc agcacttctg gtggagatat aaatattgtc aatctacggg cgaaaacctt
    25261 ttcagctaag accagcggtg gcgatatcgg cgccgataaa gttgaggccg aacttaccct
    25321 gcgtacctcc ggcggtgata taatcattgg ccagattgaa ggcaatttag aaattcacac
    25381 ctctggaggt gatatagaag tgcgtcaggt aaataagaat ttgaaggcta ctacctccgg
    25441 cggcaacatc gatattggac tcataaaagg ttattgttat gctcgtactt ctggtggcaa
    25501 tattgaggtt gaccgggcat atgatctgga agccattact tcaggcggtg atatcctagc
    25561 caaaggagtg gtcggataca tctatgcgcg taccagcgga ggtgatatag aagctcgtaa
    25621 attatttaat tctgacgtga agaataatgc cattgatttg gaaaccagcg gcggcgagat
    25681 agaagttatc ctaccggcta atcttaatgc cgatattgat gctgaaatta ccgttaccag
    25741 tcgtcgttat gccgatgtca atatccactc cgattttccg gttcagaaga gtcatgaaat
    25801 taaaagctcg cgtatataca tctatgccag aggcaaaatt aaccaaggtg gcagtctagt
    25861 caggcttagg acgattaacg gcaacataaa catattaaag gccgatcgcg aatcgaggtg
    25921 atgaaaataa tatgaaaaga aaattaatta tcctcttgat attcatgata gcggttttat
    25981 cagcggaaga ttacttcacg gaacattatg atattccgta taaaggtgaa gaagagctgg
    26041 acgttaatat cagctttggt ttggggactc ttgatctcaa aagtcacgat gatcctaaat
    26101 acctggtgca ttcaataatg acttattcac aacgtattta taagccgatt gtggattata
    26161 agatactggg caatcgtggt cgcctaaaaa tgtccaccga aaagtacgac gaatccaata
    26221 agaatgataa aagaaatctc aaatcttccc aagtaaataa agaattaaat aaatggcatt
    26281 tagaattttc tcaaaaaata ccgatttact ttgcagtgga tctcggcttg ggagagggtc
    26341 ggctggattt taccgattta cgggtgcggg atttgaaatt gcattgtggg ttgagtgacg
    26401 ttaatgtcat atttagagat aagaatcagg ataatatgcg gaatatggtt attgagactg
    26461 gactgggtag cgttaatgtt aagggtctta gttacgccaa tatggaacgc tttaacgtgg
    26521 aatgtggtct cggcactacc gaattggtgt ttgacggtag cctgagtcag gatattcgag
    26581 gtaagattga ggtcggactc ggttcggtca ttatcgaagt cccggataat gtggcggttg
    26641 aagtccgttc gcaaagttcc ttcctgtcat cgcttaattt gcatggtttt gatcgcatca
    26701 gcgaaaacct ataccgcagt ggtaactggg aaaccgccaa caaaagaatc tatctggaaa
    26761 ttgaggttgg acttggaagt gttgacatca actggttaga ttaaagctct ccgttcgttg
    26821 catatagatc acaatgcggc cattccgacc gcatttttat tttagcaata ccatgcccaa
    26881 gttgttaaac catgaaattt ggaagcagta ttattcgagg tagttttcgc ggatcaatag
    26941 gaggatatta gaatgggaaa ctatcagata tcttttgctt tcgagttcga tttcgatagc
    27001 ggacttacgc aagaaaatgg cgtaatcacc ttcttgagcc tgaagaggca aatagtgcgg
    27061 ctcatcttcc ccttcttccc agggttcatc ttcaatatcg tcggtatggg ggattgggaa
    27121 acccggacca gttttaacga tgaagccgcc ctgcaccttc tctttttctt tcactccggg
    27181 aggtaagaat aaaccgcttg gcgttttgtt ctgatcttca tcaggatcga ttaaaactcg
    27241 atcgccaatc aaaattattt catgttcgct atgcatttga taaaatttat aaatatatta
    27301 agcctaaacc aaatgctatc aaaatttaaa ccggtctatt aaaaattgta actcagcatg
    27361 gataatttta actaattttc agaatggtaa aagaaaggag taatctgtgg caaaagatgt
    27421 ggtttacgat gttgtaatca ttggcggagg accagccgga ctgacggcgg ctatctacac
    27481 cgcgcgcgaa atgctcagca cgttattgct ggaaaaatct atgacgggcg gcatcccggc
    27541 tatagttgat cggattgaaa attatccagg ctttccggat ggaataagtg gtatggaatt
    27601 aatgcaaaat atacgggctc aggcggatcg ttttggaacc aatatcagcg agtttgaaga
    27661 cgtgagcaaa attataccag gaaggcaatg ctttgaagta atttcggata atcgtcgttt
    27721 tcttgctaaa gcggtaatca ttgctaccgg aggggttccc cgcaatctcg gcatcgcctc
    27781 cgagcaagag tttttgggac ggggagtgtc ctattgtgcc acctgtgacg ggccattata
    27841 tcgcaatgcg gaattaattg tagtgggggg cggaaatgcc gctattcagg aggcgctata
    27901 tttgacaaaa tttgctcgaa gggtgacggt tattcaccgg cgcgacaaat tgcgggcgca
    27961 accggtattg caggagcgag cttttaagaa tgaaaagatc agatttatct gggattcggt
    28021 ggtttccaat atttttggag ctgagcaagt cgaaggcgtt gaaatccgca atgtcaagag
    28081 tggtgaaaaa atcagccttc cggctgaagg tgtattcatt tttatcggct gggtgccgaa
    28141 taccgagttc gtgcgtggct tggtgaatct cgatgatgac ggacatataa taaccgatgc
    28201 agaaatgcac acaaacgttc cgggaattat agcagttggc gacgtgcgct ccaagccatt
    28261 tcgtcaaatt gccaatgccg ttggcgacgg cgctgttgga gctctgtccg cttccaagta
    28321 tcttgaagga ttacataatt agcgttatcg gagatttgag ataaataatt aagcaaatcg
    28381 aatttttata aaaggaggca acgtgaaagt tcatatacca aaatcaatcg tcgtggcggc
    28441 tctttttact acgatgatag cctgtagcac aagcgagttg accagaaaga ttccgcctac
    28501 tccgccgagc ccatcccaat ctcttggatt agataatgaa attgcccttg atccgaaaat
    28561 taccaaaggg aaatttgata acggcttgac ttattacatt cgcaaaaatg aaaagccgga
    28621 aaggcgcgca gaactgcgct tagtggttaa cgccggttca gttctggaag aggaagatca
    28681 gcggggatta gctcattttc tggagcatat ggcttttaac ggtacagccc attttaagaa
    28741 gcaggaaatt atcaatttta tggaattagc cggtatgcca ttcggttcgc atctaaatgc
    28801 ttataccagt ttcgatgaaa cggtatatat gctgaccgtg cccactgacc gtgattcttt
    28861 attggaaaag ggttttcaaa ttttggctga ttgggcttct cagattagtc tggaagatga
    28921 agaaattgat aaagagcggg gtgtcattcg cgaagaatgg cggcttgggc gcggagccga
    28981 aatgcgtatc caggagaaag aatttcaggt aattttttgg ggctcattat atgccgaacg
    29041 ccatccaatt gggcaaatag ccgtaattga tacgtttcat tacgatacat tgcgaaaatt
    29101 ttatcgcgac tggtatcgcc ctgacttgat ggcagtcatt gccgtcggtg attttgatga
    29161 ggacagggtc aagaatctca tcgagcaaaa ttttattggc ttaaaatcac cagccgatgc
    29221 gccgcctcgt ccaattccgc ccgtgcctga tcataccgaa accttgtttt caattcagac
    29281 cgaccccgag ctaacccgca atcaagtgga tttgcttatt aaattgccgc gaacagaaca
    29341 aaaatatata cgtgactatc gccaatcgat tgttgaaaat ttatacaaca gtatgctgaa
    29401 tgaacgtctg gaggaattaa ccaaaactgc cgaaccacct tttctaggag cggcgtctca
    29461 aaaggggcga ttagtccgct ccaaggattg ttattccatc ggagccgttg taaaagataa
    29521 tggcattgaa gagggattta cggctattct tacggaattg aaacgcgtcc gggaatttgg
    29581 atttacagaa accgaactaa aacgggctaa aagcacagct ttacgtaata tggaacagat
    29641 ttaccgcgag cgggataaga tttactcacg cagttttgcc agtgaatata tacgtaattt
    29701 tcttgataat gaaccagtac cggggattga actcgaatat gaattttaca aaaaatacgt
    29761 tccggagata gcactgcccg aggtcaatgt tctggctaaa gaatgggtta ctgatattaa
    29821 ccgggtagtt ttagtatctg ctcccgagaa accggatttg aaaataccga ccgaaaccga
    29881 attacgcgaa attttcgaaa aggtggagcg gcaggaagtg acagcctata ttgatgaggt
    29941 ggctgaaagc gatctggtca ggcaattgcc gccgcccggt cgcatcctgc aggagactct
    30001 cattgattcc ctggatctta ctgaactaat attgagtaat ggggtaaaag ttatcattaa
    30061 gccgactgat tttaaaaatg acgagattct gctttcggct tatagtcctg gcggaacctc
    30121 actggttccc gattcaaatt atgttgccgc aattagcagt gttcccatcg tcactgaaag
    30181 cggcttggga gaattcaata gcgtcatgtt atctaaaaaa ttggcaggta aagtggtttc
    30241 ggtgcaaccg tctctggcgg aattgacgga aaatttcagt gcctcagcct cgcctgaaga
    30301 cctagaaacc atgttccaac tgatttatct ttattttacg gcgccgcgca aagacagcac
    30361 tgcctacctg tcttatctcg aaaaattgaa aaactcgatt atcaatcgcg ccgccgatcc
    30421 atttgcggct ttcagtgaca caatcagggt gacgctatct cagtatcatt tccgggcgcg
    30481 cccatggacg attgccatgc tggatgagat agatttgaat aaatcataca acattttccg
    30541 agatcgtttt ggagaagctg gggattttac ttttttccta attggcaatg tggaattaga
    30601 ttcggcgaaa cagatggcta tgcgctatct ggctaattta cctacaaata atcgcgagga
    30661 gaattggcgc gacgtaggta tccgtccacc gaacggcgtg gtgcagaagg cggtttataa
    30721 agggctcgag aagcaatcct acgtccagat taccttcagc ggtaattttg attggagtcg
    30781 tcagaatcgt tttgaattag caacggtaac cgatgttctg cgaatcaaat tgcgagaaca
    30841 aatcagagaa gaaaagggcg gcacatacgg tatttatgtt ggttcctctt ccaagcaata
    30901 cccatatcct gaatttcgtc tgactattca atttggttgc gacccggagc gagtgacaga
    30961 attaacctcc gtcatcttcc aagaaattga aaacctaaag agcgaaccac ttggcgatga
    31021 agatctggcc aaaattaggc aaattgattt acgctcctac gagactaatc tgaaagagaa
    31081 ccgcttttgg ctggataacc ttcatttcta ttatttcaac aatgaaaatc caatggcgat
    31141 cctgcattat cctcaattag tagaagatct gacgaaggaa atggtccaga atgccgcccg
    31201 acgctacctg ccgcttgata actacgtaga ggttgtactt tatccggagc agtaaaagtc
    31261 gggaactata tatgctaatc ttggttgtat ccgctcattt ttaaaggaga acaatgatga
    31321 ctaatctgga gctcaacata cttgggattg tctcagaggg cgcaactcac gcttataata
    31381 ttgagaagat tatcgccgaa cgcgggatac gcgaacgcac caacgttgga ttctcgacga
    31441 tttacgccgt tttaaacaaa atgcagaaaa ttggtcttct gatgagtaat ttggaaccac
    31501 aaaaaggact acccagccgc agaatttatt caattacacc caaggggcgt cagattttta
    31561 atgaggaaat cttcaaagcc ctttcccaac cacaacatca gccgtcactt tttgaaaccg
    31621 gtctaactta tagtggtgtc cttaataagg aggcgctccg agaggcgtta acgctctatg
    31681 ataccgagct aggtcggcag attcagaaca aagttcatga actgaccgct ctctcaaata
    31741 ccaatgacca ggttcagcgc gccctattaa cccgaccgct ggtcattttg caggcagagc
    31801 gcaagtggat tcgcgaatta atgggattac ttttaaccta aagtgggagg gattattatg
    31861 gatgtgtgga ttagctacct aacttctcat ccgattatcg gagtcgcgct gactgccgca
    31921 ttggtgttat ttatcataat ggttatagcg cgcctgctca agtgggcagt catctcattt
    31981 atcattctgg cgctagcagt tggcttgaca tacaaaatcg ctcagccgaa aaaaatcatt
    32041 gaccggattc aaaaagatat agagtccgta acagaaaatg actctgaaaa ggttaccgat
    32101 aaacttcaaa agagcactga agaagccatt gagaaggtca aggaaagttt caaaaaagag
    32161 taatt
//
LOCUS       DAOOMU010000014        31846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1189_length_31846_cov_23.461829, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000014 DAOOMU010000000
VERSION     DAOOMU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 31846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31846
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_31846_cov_23.461829"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="PLI55_02430"
     CDS             complement(<1..1449)
                     /locus_tag="PLI55_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPA99725.1"
                     /translation="MTQTGLDQETRTMILDTINSLKNELLTREKILELDDKNEFPLEI
                     VKELLSDRIGLQMVFIPEEFGGLGGGALDTYYVSVELAKICLGVATAFLAIHLGAEPI
                     LLFGTEEQKQKWLAKIVNEAAIVAYAVTEADAGSNLDAIKTKAEPMTDESGNIVSYRL
                     NGSKQFISNGGYADFLTLLAKAPEGATFFIVDGKSTGLKRGKPEHKHGIRAANTSPLT
                     LENVIVPAENIVGGVPGVGLKQANDVFSYTRLMVAAFGLGAGLSAMEKAIQFAKTRIQ
                     FGTPLIEKQGYTHKLLVPNVVRLTAAQAYIEEIAKRVDSGDTDILVEGSIAKYFATEA
                     GNIAAEAAIQALGGYGYMHDYEVEKIKRDVRITMIYEGTSEIQQNIIYLYRWRTTVKS
                     KGEFYQKMAQEMRTIAEQSNLESAKIVAQALELLNDLIMFAHNQKLTRQQHIQFLLSD
                     VITWSEVAAALVRKTAEHLRDALPDAIQLQSVL"
     gene            1635..4451
                     /gene="uvrA"
                     /locus_tag="PLI55_02435"
     CDS             1635..4451
                     /gene="uvrA"
                     /locus_tag="PLI55_02435"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPA99726.1"
                     /translation="MSTEHIVIKGAREHNLKNIDVKIPRDKFVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGLMEKPDVDFIEGLSPAISIEQKSASRNPRSTVGT
                     VTEIYDYLRLLYARIGIPYCYQCGKRIERQTTQQIVDNILSWPEDTRLMILAPLVRGR
                     KGEYREVFREVRREGFLRVRIDGKIYEVEHTPVLDKNKKHNIEVVIDRLSLNPTVKDR
                     LTESVELALKIGSGLLVVVINSENEQIFSEKLACTDCGISYEELEPRMFSFNSPYGAC
                     PTCDGLGTLMEIDPALVVPDETKTLSQGAIVPLGEQPRGGWYSAILKGLARKYNFDFV
                     TPFKKLPQEAKNALLYGSDGTKLSINLKSDRIDGEYHTPFEGVIPNLRRRYKQTTSTG
                     IRMWIQQFMSYRLCPQCQGARLKPESLAIKIGGFAINQLSAMSITKLREFFVNLELNP
                     TEQQIAHQILKEIHTRLNFLINVGVEYLTLDRMTHTLSGGEAQRIRLATQIGSQLMGV
                     LYILDEPSIGLHQRDNRRLIETLLHLRDNGNTVIVVEHDREMIESADYIIDLGPGAGA
                     TGGNVVFAGTPAEIKNEPRSLTGQYLSGAKTIPIPRNRRPGNAKQISLYGATGNNLKN
                     IDVCFPLNKFICITGVSGSGKSTLINETLFPILHRNFYGSQKVPLRYTDVQGLEYIDK
                     VIAIDQSPIGRTPRSNPATYTGTFTHIRDLFANLKESKIRGYTAGRFSFNVRGGRCEV
                     CGGDGIKKIEMHFLPDVYVLCEECKGLRYNRDTLEVKYKGKSIADVLNMSISEAFEFF
                     RNIPSINKKLQTLIDVGLGYIKLGQQATTLSGGEAQRVKLSTELSKVGTGKTLYILDE
                     PTTGLHFDDIKMLLNVLNRLVDQGNTVIVIEHNLDVIKTADYIIDLGPEGGDNGGYIV
                     CEGTPEEVSAHPASQTGKFLAKIL"
     assembly_gap    4723..4822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4897..6075
                     /locus_tag="PLI55_02440"
     CDS             4897..6075
                     /locus_tag="PLI55_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPA99727.1"
                     /translation="MFKEKIRQAIEILKEQGVDMWMTFVRETETLPDPCMDLIVGTNC
                     TWQSAFIITAKGQTIAIVGSLDKERIKQTGLYDDIRIYVDDFGAAFLNALNEINPNRI
                     AINTSINDYMADGLTHGMYELLMGYTKNTPYRERFISSEKIVAALRGRKSPTEIQRIK
                     RAIEITEKIYAEISPQIKSGRTEKEIADIILKMVAELGVQTAWESNSCPAVFTGPLSA
                     GAHAEPTERVIQPGHVLNIDFGVKKDGYCSDIQRTWYILRNNEETAPEPVQKGFAVLL
                     ESVRRAVDAIRPGKMGYEIDQIARDYITANGYPEFPHGLGHQVGRSTHDGAGMLGPRW
                     ERYGDTVYLPLEIGQVFTIEPRLPIEGFGVATVEEMIVVTADGCEYLSHPQNQLFYSR
                     "
     gene            6402..8477
                     /locus_tag="PLI55_02445"
     CDS             6402..8477
                     /locus_tag="PLI55_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPA99728.1"
                     /translation="MSAKVKSLTKRISSFFFSLRMQIANNPLNEYIRLLFFAFLIGVL
                     AGLVTLAFYKLIHLISYFLYDYLPARYNNLPKWTMALFPAVGGLIVGLMIKLWPRQPR
                     QRGVVEVIKASFIGRGVIRVTTIIFNFIATAIFLGTGGSLGPEAPVAFLGAGSGSLIG
                     QAFRVSENQRRVFLAAGTAGAIAAVFNAPIGGVFFAIEIVLFNNFQTLTFSALIISAI
                     TADYISRVFLGDQLLLTLPKVEMALSLNILYFAVLGIITGLFSLAFIRFSGKMHNFFD
                     AQKGRFAPLLRLTGLMLILGIVGLFFPQIIGIGYDAIQEIANNNYTWQILAIVFVLKF
                     LFSGLLSAAGGFGGLFAPSLLMGAALGAFLAEGGNQVLGLSLDVAGFAIAGMGGMLAA
                     VNSIPVTALLIIVEITGNYHLVLPLMLTVIMSYIIVHYVLKETVYSIELRQRGIQLFD
                     GKELNVLKNVKIDQIMSYEFHKVYSTMDVKTLVKVIAEGAANIYWVENEQHKIIGFIS
                     WSDLKPLITDYQNLNTLVLVSDVMNPNVVSVKTDDTLDYVMKLFGRYNVDELPVTDPA
                     TGMLIGTVNRNRVIQLYNQEIFKRETAGDLATHIRYLDQTRFVEIDEDFSILEMSAPE
                     SMVNSTLKALDLRNRYHAQIILIKRLDNKGRRMVITPRADTIILPNDILILAGPTKKL
                     YELKERYYL"
     assembly_gap    8493..8592
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8593..9128
                     /locus_tag="PLI55_02450"
     CDS             <8593..9128
                     /locus_tag="PLI55_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPA99729.1"
                     /translation="EIEQISALRNPEGVLAIAKIPSEVNLLAPLDYPALYLWQINDPG
                     NLGTILRTALWFDVKTILLSPDSVDVYSPKVVRGAMGALFRLKVRTEVDLSRIEMILG
                     RDNAQMWAADMSGTIEKPEITNDRFVIAFGSESHGLPPEILQRATGLIRINKYGYGES
                     LNVAVAVGIILNNIRQK"
     gene            9140..9685
                     /locus_tag="PLI55_02455"
     CDS             9140..9685
                     /locus_tag="PLI55_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPA99730.1"
                     /translation="MAFLGYVLAIILLSVTPASPQVTVDTLAIGTVAPSFYASVLDGS
                     DFFLSHKVGAKAHPNEKAPVILVFFTTFCIPCRQEIPFLDSLRIEYPQIAFYLVNIGE
                     EPQIVKEYIQRMNYKMPVLLDRYGQVAKRYQASITPTLVGINVNGQIAFLKRGFQGSD
                     KELIQTNIKKLTDANLFIEKN"
     gene            9697..10596
                     /locus_tag="PLI55_02460"
     CDS             9697..10596
                     /locus_tag="PLI55_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPA99731.1"
                     /translation="MINNHQYFEPELKPHPPIIVPKIKTAIGLLIMGLICATLVSLMI
                     MIFVPTSNLTKTTQLLLLIAELFLPVPIFFWAWRRHTNLGELLRIKRVSSPSIIASMI
                     VSVSLTVIIDELDRLIQIVLPLPESFASIGEIMLITDWQSALLIIGVVVFAAPLVEEM
                     MFRGFFQRILEYRQGDITKSVLLSAMVFALVHFNPWWVVQIYLMGLFLGYLAWRANSI
                     WPSFVLHAVNNGWSVWWSHQPAFAKFYEWHGHVHPVLIIASLVCFYLGMRFFIKVTPV
                     VEKRQDIVLIENLSDYYENEPPE"
     gene            10615..11751
                     /locus_tag="PLI55_02465"
     CDS             10615..11751
                     /locus_tag="PLI55_02465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA99732.1"
                     /translation="MIIIHLITFISIVIYLVSLIIFRWGLNHPRQQRTADLPTISIVI
                     AVKNEAVNLPRLIEQLTGQNYPDGKLEIIIVDNDSTDETWAILQNEAARISNLRIFTT
                     RTEGTPYRHKKAALAVGIREARGEVILTTDADCSMGKDWARTMASYFTDDVGIVVGFS
                     AIRYLNDWFSRLQALDYLQLMAAAQGSTNLGLAWACSGQNWAFRKVLFENAGGYMLVR
                     DRVGGDDSLFMQVMQKRTRTKVVFAADKAAWVETQAADSLTTFLRQRIRWASEANYMH
                     HFNCLFFVVILATFLANLMPIVYFVLAITGVVTFVPFLIVIAIKLIGEAMLAFKAIRV
                     YQRKDLMGVFIPWFIIQPFYIVLMGILSFFGNRLGWGERETRSA"
     gene            complement(11763..12857)
                     /locus_tag="PLI55_02470"
     CDS             complement(11763..12857)
                     /locus_tag="PLI55_02470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA99733.1"
                     /translation="MLNLIEFAILLTFFYLIIMGVIGIGLIFIRTQKRVFQTLAVSVI
                     VCARNEGQIISRLLQSLTNLRYPEDKYEIILVDDDSTDDTLSIMNDFASGKSNWKVLH
                     HDKTTDQLKGKKGAITLGVQNSCGEIILVTDADCVVPPNWIASMVSYFRKEVGMVLGH
                     SPVARRKGFMNIYERFDTLCEASVAAASTFYNKPTHANGRNLAYRKKAFEDVNGYYRS
                     ANTDTGDDFFLMQEIRTKTRWRFAYNIDPASYVTTEPTKFGKKYIHQQLRRNSKAFNL
                     TIPFFILGVIVFLFHVVLAIMLFSPSLWNWLGMMLFFKFMAEFIAVYHGAHLFHQTDL
                     LKYFPILWVIYPVCYLLSQVIGSLKIYRWK"
     gene            complement(12857..14536)
                     /locus_tag="PLI55_02475"
     CDS             complement(12857..14536)
                     /locus_tag="PLI55_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99734.1"
                     /translation="MRNIRFVNLTFIIIIAFPLVVSAIGIDVPATDPVYDFLKRLETR
                     GVIKGFNDLTLPLTRDEIVNFLQIASQKADELSAIEKKILHEFLADYRYETERQLTEP
                     DSISAPFYQKNYFRQATANLFSRQDAVEENHLLMYESGETFFWFDVGARGRMDWKENH
                     QKQMFSDRYLLRGALGKSFTFHTYFARFMKNGNPAFTEPYEEEIGNFSMVQPGGTVTF
                     DILQTSLVFHQKYFNIGLYRQPVAWGASGTNNLILSKSAPLFSYIGFDTKFKGIKLSM
                     IHGSLLNDSTHYRDIAFQQRNRSKYIAAHRLDIPFFRGTTTVGFSEMVIYGDREIDIA
                     YMTPFGFYWPIGHALEDRDNTLMAMDVKTTFIRNITFYGSVLIDEMRFSELGKQWWAN
                     KHAWQAGMRWSPNIATLPFDFQAEFTAVRPWTYSHKTLTTNYTHNSICLGFPYGANSQ
                     TWYFKIGTALSRRLTLTSEYLILKKGVDDSTHFWGGDATVSYELRDEQYDHATKWLMG
                     DIETTNQLKVAGRYEIFNDFFIKAGINLYNTKLNNDQESYRFGFIETEINF"
     gene            complement(14566..15339)
                     /locus_tag="PLI55_02480"
     CDS             complement(14566..15339)
                     /locus_tag="PLI55_02480"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020029636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HPA99735.1"
                     /translation="MKEKIEIARSWLPRYTGLPLEKYGDYILLTNFRNYVDIFCAMFK
                     CDLYGENRPMQAATNSAGLTIINSGIGSPNAATIMDLLVAVHPKGVLFLGKCGGLKKS
                     TEIGHFILPIAAIRGEGTSNDYFPPQVPALPSFKLHKFVSEIIVQRGLEYRTGVVYTT
                     NRRVWEHDQAFHQRLREMTCIAIDMETATLFIVGHYNEIARGALLLVSDLPTTPEGIK
                     TEELDKQVTAQWSQVHLQIGIDAMTNIGRMGEPIKHFRY"
     gene            complement(15353..15763)
                     /locus_tag="PLI55_02485"
     CDS             complement(15353..15763)
                     /locus_tag="PLI55_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPA99736.1"
                     /translation="MRSWTLPALIALILWGFWGFLPKVATRYIDPSSALVYQAIGGTI
                     VGLGVLLSVRSQIQWQAVGAFWAILTGLFGMLGSLFFLQAVSRGKVTTIVTMTALYPL
                     ISIVLALIFLKEPFSFRQGLGVFCALMALILFSI"
     gene            complement(15897..16235)
                     /locus_tag="PLI55_02490"
     CDS             complement(15897..16235)
                     /locus_tag="PLI55_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPA99737.1"
                     /translation="MKIAIPLANGKLTLHFGHCEQFALVDVEVQNKKIIKRTDLIPPP
                     HEPGLLPSWLAEHGVNLIIAGGMGQRALMLFAENNIQVLVGACSESPEKLIGDYFDGN
                     LQLTSNVCDH"
     gene            complement(16254..17162)
                     /locus_tag="PLI55_02495"
     CDS             complement(16254..17162)
                     /locus_tag="PLI55_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPA99738.1"
                     /translation="MSDISKEKTKNTCDSCGNSTCSAAKPREGENQQEFKERQQLLSR
                     LCHIRHKIIVLSGKGGVGKSTVAVNLAVALMLAGKRVGLLDVDIHGPSIPTMLGLEDE
                     VIQKDDDGMIPIELGDLKVMSIGFMLRNPDDAIIWRGPLKMGIIKQFLKDVSWGELDF
                     LIIDSPPGTGDEPLSVCQLIGDLDGAIVVTTPQKLAAVDVRKSITFCRHLNVKVLGIV
                     ENMSSFVCPKCGEVTPIFGTGGGKKIAESMQVPFLGSIPIDPKIGQTGDSGQVFIHQY
                     SKSPTAEIMRQIIKPILDMDSENVKS"
     gene            complement(17166..18047)
                     /locus_tag="PLI55_02500"
     CDS             complement(17166..18047)
                     /locus_tag="PLI55_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA99739.1"
                     /translation="MQELVIISGKGGTGKTSIAAALAVLAKSAVVADCDVDAADLHLV
                     LSPQLKERHDFFAGHEAVIRQADCIACGDCLAHCRFEAILTRQEASAGNKFFVDPIAC
                     EGCGVCVQFCPVKAIDFPDRLCGEWFVSATRVGPLVHAKLSIAAENSGKLVSTVRQSA
                     RRLAESEKRALVIVDGPPGIGCPVIASITGATQVLVVTEPTVTGEHDLERVLALTRHF
                     NIPTSVCVNKWDLNPGMTEQIEKKARKSSAKTVGRIRYDLSVTKAQLQALSVVETDSP
                     CAMDIKNVWNKLGLTGD"
     gene            complement(18059..18379)
                     /locus_tag="PLI55_02505"
     CDS             complement(18059..18379)
                     /locus_tag="PLI55_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HPA99740.1"
                     /translation="MKLGMIITQTDAETVFNGLRLALYSLEQGDEVRIFLSGKGVEID
                     QIEDSKFDVKTLAQKVIDKGGQFLACGACLKLRNSDGSEVCPLSTLKDQYQIVRDSDK
                     IVTI"
     gene            complement(18420..19133)
                     /locus_tag="PLI55_02510"
     CDS             complement(18420..19133)
                     /locus_tag="PLI55_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPA99741.1"
                     /translation="MQFLVLYDNSSLSPAIRCGWGFACLVDKRILFDTGDKYEPLCEN
                     LAQLKINPKTIEAVVISHDHHDHTGGLWGLLERQPGLKVYACPGFGNDFKQRVNALRG
                     KLIESIGFRKIDDHIGVTGEIPAQYKGSPKPEQALIARTARGIAVVTGCSHPGIITIL
                     TKVKTIFPAEKIALILGGFHLRDSACEAIEEIASQLVTVGVQKVSPAHCTGEPAQAIF
                     KRRFGANYVPAGAGNQIEI"
     gene            complement(19156..20133)
                     /locus_tag="PLI55_02515"
     CDS             complement(19156..20133)
                     /locus_tag="PLI55_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013468461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA99742.1"
                     /translation="MNLIDLQYAYGPVPSRRLGKSLGINNIPAKICTYACAYCQVGKT
                     DKMPVERQAFYKPEDIFRNVKYLLDNARHKGEQVDFLTFVPDGEPTLDINLGREISML
                     KTLGVPVGVITNSSLIWRTDVREEMNKADWVSLKIDTVDEAIWHRINRPNRSLDLQKI
                     LDGILQFARHYQGQLVTETMLIRDHNSSQAHIDRLVEFIAQLPDITAYLSIPIRPPSQ
                     KWVQAPTEEILIQAYYIFSSKIRNVKLIIEYEGNDFAFSGNVEADLLNITAVHPMREE
                     AIAKLLEKAKSDWSVVDSLIEKELLVKKEYRGKTFYTRRIKSLNDEIRK"
     gene            complement(20134..20742)
                     /locus_tag="PLI55_02520"
     CDS             complement(20134..20742)
                     /locus_tag="PLI55_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPA99743.1"
                     /translation="MLEKQKSFQSRTAQYLRLGYDRFAASDFVIATRGDLSSPALDIG
                     TGKGLTAMALAKKGLDVISVDIETDEQAFALFLAREAGLEHRIKFIGQNAAELPYPDN
                     YFRCVVLSNILHHLENAAPVLEEAARVLAPRGIMIIADFSAEGFEIVARLHREDGHLH
                     SVSGETLKTAERFLNTKGFKTTNCLSGHMQEIIVLTQNDGIE"
     gene            complement(20787..21623)
                     /locus_tag="PLI55_02525"
     CDS             complement(20787..21623)
                     /locus_tag="PLI55_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPA99744.1"
                     /translation="MIIAVASGKGGTGKTTIAVNLAYALDREVQLLDCDVEEPNAQLF
                     LNGALLSTEEVTIPIPQIDASLCNGCGECSRFCEYNAIVSYGTMPLVFADMCHGCGGC
                     MKVCPPQAIREVDYRIGVIETFSAGKITLIQGNLDIGAAMAPPLIRAVKRKTDNRIPI
                     LLDAPPGTSCPVITTLRGADFVTLVTEPTPFGLNDLILAVETVREMDLPFGVIINRSG
                     SGDARVNFYCADENIPILAEIPDDRKIAEAYSRGELIGKVFPEYRLIFEKIINDITTR
                     SD"
     gene            complement(21620..22495)
                     /locus_tag="PLI55_02530"
     CDS             complement(21620..22495)
                     /locus_tag="PLI55_02530"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HPA99745.1"
                     /translation="MDLDCIPCFVQQAIRAARHLSDDPDVQERIVREALCCIGAVDFR
                     EPPPRASQIFYRRLSEITGIPDLYQADKDRQNRLALKLLPALKEQIGISPDPLLLAAR
                     LAIAGNIIDLGANGNLTIADIHEAVDQALNEQLVGDWEKFKSAVSEARDILYLADNAG
                     EIVFDRLLIEQILPKPVTLVVRGAPTINDALQTDAKAAGLDKLVDIIDNGSDAPGTLL
                     GDCSREFLRCFATADLIIAKGQGNYETLCNEPRNIFFLFKIKCPVIARHIGHPVGTQV
                     LLKSNEIQPENRSIH"
     gene            complement(22501..22983)
                     /gene="tsaA"
                     /locus_tag="PLI55_02535"
     CDS             complement(22501..22983)
                     /gene="tsaA"
                     /locus_tag="PLI55_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HPA99746.1"
                     /translation="MRIASVSFRPIGIIHSQHQNAEQIPIQPVYAKDCPAKVEVYQKF
                     AEGLRDLDGFSHIYLIYHFHRAKPAKLRVKPFLQDVERGVFATRSPNRPNAIGLSIVE
                     LIRREGNILHIKGADILDGTPLLDIKPYIKRFDRIDNSRDGWQEEIDEQTAQIRGRRK
                     "
     gene            complement(22980..23342)
                     /locus_tag="PLI55_02540"
     CDS             complement(22980..23342)
                     /locus_tag="PLI55_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPA99747.1"
                     /translation="MKIAFTTSGNDLNAPLDNRFGRAEKFLIYDLENDTFEIIDNRQN
                     LNAAQGAGIQSAETIVRSGAKALVTAHCGPKAFRVLQSAGVKIYTTSAATVASALADY
                     RAGKLSESKSEDVAGHWI"
     gene            complement(23422..24135)
                     /locus_tag="PLI55_02545"
     CDS             complement(23422..24135)
                     /locus_tag="PLI55_02545"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPA99748.1"
                     /translation="MEPINETQSVSMPRIGDPAPAFKAITTQGEINFPQDYRGSWVIL
                     FSHPADFTPVCTSEFMTFATMEKQFAEVNCKLVGLSVDGLYSHIAWLRTIKEKIDYKG
                     MKNVEVKFPLIEDITMEVAKKYGMIQPGESNTKAVRAVFVIDPKGIIRTIIYYPLSLG
                     RNFDELLRVVIALQTADAFNVSTPADWRPGDDVIVPTAGSCGVAKERMESKEGIKCLD
                     WFFCLKEITKEDVWKAITK"
     gene            complement(24251..24754)
                     /locus_tag="PLI55_02550"
     CDS             complement(24251..24754)
                     /locus_tag="PLI55_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HPA99749.1"
                     /translation="MGTKAIEILNLDVKHLVDLLNRAYADEWLAYYQYWVGAKVVKGP
                     MKGAVIAELEEHAADELRHADMLVKRIIQLGGTPLIKPEDWYKHTNCGYDAPDDPHVK
                     EILEQNIKGEQCAITVYKKLFDLTAGKDPVTYNMVLEILEDEIEHEDDLQAELEDFEM
                     LVEKHSK"
     gene            complement(24853..25206)
                     /locus_tag="PLI55_02555"
     CDS             complement(24853..25206)
                     /locus_tag="PLI55_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HPA99750.1"
                     /translation="MPRGDGTGPTGMGPMTGRAAGFCAGNNVPGYANPYIGRGFGGGM
                     GRGRGRGFRHWFYATGLPRWARLGNINPAYAYPAPYQKLDPEVERQSLQTQAEYLEAE
                     LNEVKKRLDALKTDK"
     gene            complement(25239..25832)
                     /locus_tag="PLI55_02560"
     CDS             complement(25239..25832)
                     /locus_tag="PLI55_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPA99751.1"
                     /translation="MKVAFAIWNNRIAPVFDVARLLHIVEAENSVINRSVEEILPIDL
                     LPIKKAERLRELGVKTLICGAISRPLYDTIVANGIDVIPFIAGELDEVVQAWISGRPD
                     WHHFAMPGCCGRRGRWQGGFHTNYQEMFIMSGKRRGMGSGQGRGGQRFGRMGGGSAAG
                     PSGNCVCPECGHKEPHQRGMPCFEQKCPQCGAAMARE"
     gene            complement(26020..27381)
                     /locus_tag="PLI55_02565"
     CDS             complement(26020..27381)
                     /locus_tag="PLI55_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPA99752.1"
                     /translation="MTLQSALVENILESISDGVFTVDENWCITSFNRAAEEITGVKRK
                     EAIGQRCSEVFRSSLCGADCAMQKTLKTGKAVIGKSAYIIKADGARIPISISTALFKD
                     KNGKIIGGAETFRDLSEIETLRQELRNKMGFGDLVSRSPLMQKIFEVLPAIAASPSTV
                     LITGETGTGKELVARTIHQLSPRSRGPFIAVNCAALPNELLESELFGYKKGAFTGAVQ
                     DKPGRFALAKGGTLFFDEIGEVSPALQVKLLRVLQDRIYEPLGATRSETANVRIIVAT
                     NQDLTEMVKTGAFREDLYYRINVVRIDLPPLRRRKEDIPVIIEQLVERFNLLWRKSVS
                     GVSIEALSLLMAHDWPGNIRELENVIERAFVLCNDGEITLKHLPEELTARRVTDLPPE
                     SIRSAVQTLEAQTIQSALARHNFNRTAAAKDLGIHKTTLFRRMKKLGLSLPRKDGRSH
                     PKK"
     gene            complement(27550..27693)
                     /locus_tag="PLI55_02570"
                     /pseudo
     CDS             complement(27550..27693)
                     /locus_tag="PLI55_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006492658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
     gene            complement(27703..27843)
                     /locus_tag="PLI55_02575"
     CDS             complement(27703..27843)
                     /locus_tag="PLI55_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99753.1"
                     /translation="MSNKIPFKARAISSQTAYFLAELASVASLSGQEEKVIARRMKLT
                     AP"
     gene            complement(27840..28688)
                     /locus_tag="PLI55_02580"
     CDS             complement(27840..28688)
                     /locus_tag="PLI55_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008462394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HPA99754.1"
                     /translation="MKFNIKSKIPTIICEWTIVFLFGAGNFLSAQVDTLVIPSVAMSK
                     NPKAVVVLPDVYHKSNDRFPVVYLLHGWSGSYRDWPTKMDLKPLADKYGFIIVCPDGG
                     YAGWYVNSPIVKDSQYETYIAKEVVDFIDANFRCVADSTGRFLCGLSMGGHGAFTLLA
                     KFPERFAGAGCMSGAMTMSSGERRAGLADVLGDWETNQQRWEANSALFLAERLVGHDK
                     AILIDCGVDDFLHQTNFLLHKKLLDLKIPHVYIERPGAHTWDYWTNALEYHLLFFRNN
                     FYKDAK"
     gene            complement(28990..31740)
                     /gene="ppdK"
                     /locus_tag="PLI55_02585"
     CDS             complement(28990..31740)
                     /gene="ppdK"
                     /locus_tag="PLI55_02585"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012414518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPA99755.1"
                     /translation="MTTQKSKTPKYVYFFGGGKADGNESMKELLGGKGANLAEMAGHP
                     DLRLPVPPGFTITTEVCTYYYQHNRKYPAELKEQVEAALAKVEKIMGRKFGDPDNPLL
                     VSVRSGARKSMPGMMETVLNIGLTEKTIPGLIKQSGGNERFVYDAYRRLIMMYSDVVM
                     EKAAGIEPKDDEGIRRQLEKIMDDFKKAKNYTNDTDLTADDLKELCRVFKEQVKKVLG
                     KDFPDDPYEQLWGGIGAVFASWNGKRAVAYRRIEGIPDDWGTAVTVQAMVFGNMGDDC
                     ATGVAFSRNPGNGENQFYGEYLINAQGEDVVAGIRTPAPINKYSASEHNKDLVTLEEY
                     MPAIYKELVEYRNRLEKHYRDMQDIEFTIEKGRLFMLQCRIGKRNGPAAVRMALDMLN
                     EKLITKEEAVLRVSPSQLDELLHPIIDPKHEKKIEPLAKGLPAGPGAATGQIVFSSAD
                     AVEWAKAGKKVILVREETNPEDVDGMRASQAILTARGGMTSHAALVARGWGKCCIVGC
                     SAIHIDYQKKVMKIGDRVFKEGDFITLNGTKGYVYAGTLPMIDATENPDLVKFLKVCD
                     SVRRLGIRTNADTPADAKRARQFGAEGIGLFRTEHMFYGEGSDAPLFALRKMIVSNTL
                     DERQKALDELFPYVKKDIKATLEAMDGYPVVIRLLDPPLHEFVPREKEKQEKLARDLN
                     ISLDELEKRAAELHESNPMMGHRGVRLGVTYPEVSRMQIRAILEAAAELLKEGKKPYP
                     EIMIPVVSLTKELEHQKAIVDEVYQEVLKKHGLKEIKYLFGTMIEIPRACLVANEIAG
                     VTDFFSFGTNDLTQMGFGFSRDDIGSFVPDYIEKKILPEDPFQTIDQVGIGELIQIGI
                     ERGRKTKPNLEIGICGEHGGEPRSVKFCHRVGMDYVSCSPFRVPIARLAAAQAVAEEK
                     TK"
BASE COUNT         8496 a         7395 c         6862 g         8893 t
ORIGIN      
        1 cagaacggat tggagttgaa tagcatcagg caatgcatca cgtaaatgtt cggcggtttt
       61 gcgtaccaaa gccgcggcaa cttccgacca ggtgataaca tccgaaagca agaattgaat
      121 gtgttgctgg cgagttaatt tctgattatg ggcgaacatg atcaggtcat tcagaagctc
      181 caacgcttga gccacaattt tggcagattc caggttcgat tgttccgcaa tagttcgcat
      241 ctcttgagcc attttttgat aaaattcgcc tttggattta acggttgttc gccagcgata
      301 caagtagata atattttgct ggatttcact ggtaccttca taaatcatag tgatgcgcac
      361 gtcgcgtttg attttctcaa cttcataatc gtgcatataa ccatagccac ccagagcttg
      421 aatagcggct tccgcggcta tatttccggc ttccgtcgca aaatatttag caatcgaacc
      481 ttcgaccaga atatcagtat cgcccgaatc cacccgcttg gcaatttcct cgatatatgc
      541 ctgcgccgcg gtcaaacgga cgacattagg taccagcaat ttatgcgtat agccttgctt
      601 ttcaatcaat ggcgtcccaa actgaattcg cgttttggcg aactggatgg ctttttccat
      661 agccgaaagc ccagctccga gtccaaatgc cgccaccatc agccgcgtat aggaaaagac
      721 atcattagcc tgttttaatc caacgccggg taccccaccg acgatatttt cagccggcac
      781 tattacattt tccagggtga ggggcgaagt atttgccgcc cggatgccat gtttatgttc
      841 cggtttgccg cgtttcaatc ccgtggattt gccatcaact atgaaaaaag tcgcaccttc
      901 cggagccttt gccagtaaag ttaaaaaatc ggcataccca ccgttggaga tgaattgttt
      961 actaccattc agacgatagg atacgatatt cccagattca tctgtcatcg gttcggcttt
     1021 ggtcttgatt gcgtcgagat tgctaccagc atcagcctca gtcacagcat aagctacgat
     1081 tgcggcttcg ttgacaattt tagcgagcca cttctgcttc tgctcttcgg ttccaaacaa
     1141 aagaatcggt tcggcaccaa gatgaatcgc cagaaaagcc gtggcaacgc caaggcaaat
     1201 ttttgccagt tccaccgaaa cgtaataagt atcgagtgcg ccgccgccca acccgccgaa
     1261 ttcttcagga atgaagacca tctgcaaacc gatgcggtca ctcagcagtt ccttgacgat
     1321 ttccagcggg aattcgtttt tatcatcgag ttcgaggatc ttttcgcgag tcaacaattc
     1381 atttttcagt gaattaatag tgtccaagat catcgtccga gtttcctgat cgaggccagt
     1441 ctgggtcatt taacttctcc tacattgatt taaactaaca tagaaatata atcagtcatt
     1501 agtgattgaa gaaagaaaaa gtgtgaaact ttattactaa aacggaaaat aaatttgtag
     1561 aaaattcgtg aaaaatattt ttcattatgg taataaggtg ataatttaat cggataattt
     1621 aaatggaaag tctgatgagt acagagcata tcgtcatcaa gggcgcccgc gaacacaatc
     1681 tgaaaaatat tgacgtaaaa atcccacgtg acaaattcgt ggtcattacc ggtttatcag
     1741 ggtcgggcaa gtcttcactg gcttttgata caatatatgc cgaaggtcag cgacgttatg
     1801 tcgaatcgct atcagcctat gcccgccagt tcttgggatt aatggaaaaa ccggatgtgg
     1861 actttatcga agggctttcg ccagccattt ctattgaaca gaaatcggcg tcccgcaatc
     1921 cgcgttcaac cgtaggaacg gtgactgaaa tttatgatta cctgcgtctg ctgtatgccc
     1981 gcatcgggat tccgtattgc taccaatgcg gtaaacgtat tgaacgtcaa actactcagc
     2041 aaattgttga taatatactt agctggcccg aagacacccg cctgatgatt ttagcgccct
     2101 tggtgcgtgg gcgtaaaggt gaataccgag aagtgtttcg cgaagttcgt cgcgaaggct
     2161 tcctaagagt tcgaattgac gggaaaatct atgaagtcga gcacacgccc gttcttgata
     2221 agaacaaaaa acacaatatt gaagtggtca ttgatcgcct cagtcttaat cctactgtca
     2281 aagatcgtct gactgagtcg gtcgaactgg cgcttaaaat cggctccggt ttactagtag
     2341 ttgtgatcaa tagcgaaaat gaacagattt tcagtgaaaa attagcctgt accgactgcg
     2401 gaatcagcta cgaggaattg gaacctcgta tgttttcctt caatagtccg tatggagctt
     2461 gtcccacttg tgatggtctc ggaactttga tggaaataga cccggcatta gttgtaccgg
     2521 atgagaccaa aacactcagt cagggtgcca tcgtaccatt gggcgaacag ccccgcggcg
     2581 gctggtactc ggcaatacta aaaggactgg cgcgcaaata taacttcgac tttgttacac
     2641 catttaaaaa attacctcag gaagctaaaa atgctttgtt atatggttcc gatggtacca
     2701 aactttccat taatttgaaa tcggatcgaa ttgacggcga gtatcatacg cctttcgaag
     2761 gagtaattcc taatttacgc cgtcgctata agcagacgac ttccaccgga attcgcatgt
     2821 ggattcagca atttatgagt tatcgcttat gcccccaatg tcagggagcc cgtttaaaac
     2881 cggagagtct ggcaataaaa atcgggggat ttgcgatcaa tcagttgagc gctatgtcaa
     2941 ttaccaagtt gagagaattt ttcgttaatc tcgaactcaa cccgaccgaa caacaaattg
     3001 ctcatcaaat tttaaaagag attcacacta ggctcaattt tttaattaat gttggggtag
     3061 agtacctaac tctggatcgt atgacccaca ccctttccgg cggcgaagct caacgcattc
     3121 gattggcaac tcaaattggt tcgcaattaa tgggcgtgct gtatattctc gatgaaccca
     3181 gcatcggttt gcaccaaagg gataaccgcc gattaattga aactcttctt catctgcggg
     3241 ataatgggaa cacggtaatt gtggtggaac acgaccgaga aatgatcgaa tcggcggatt
     3301 atataattga tctcggaccg ggagccggcg ctactggcgg caatgtggta ttcgctggaa
     3361 ctccggctga gattaaaaat gagccgcgct cactgaccgg tcaatatctc tccggagcta
     3421 aaacaatccc aataccacga aatcgccgcc ctggcaacgc caagcaaatc agtttatatg
     3481 gcgccaccgg caataatttg aaaaacatcg atgtgtgttt ccctttaaat aaattcattt
     3541 gcattaccgg tgtatctggt tcaggcaaaa gtaccctaat caacgaaaca ttatttccaa
     3601 ttttgcaccg caacttctat ggttcccaga aagtaccact gagatatacc gatgttcaag
     3661 ggctggaata tattgacaaa gtaatcgcta tagatcaatc gccgatcgga cgaacaccgc
     3721 gctccaaccc ggcaacttat actggcactt ttactcatat ccgcgaccta tttgcaaacc
     3781 taaaggaatc aaaaatccgc ggctacaccg ccggtcgctt ttcattcaac gttcggggcg
     3841 gtcgttgtga agtttgcggc ggggacggaa ttaagaaaat tgaaatgcac tttttgccgg
     3901 acgtttatgt tctttgcgaa gaatgcaagg gtctgcgtta taaccgcgat accctcgaag
     3961 ttaaatacaa agggaaaagt attgcggatg ttttgaatat gtccataagt gaagcctttg
     4021 aattttttag aaatatccct tctatcaata aaaaacttca gactctaata gacgtcggct
     4081 tgggttatat caaacttggc caacaggcta cgacattatc aggcggcgaa gcccagcggg
     4141 taaaattatc aaccgaactg agcaaagtag gcaccggcaa aaccctctac attcttgatg
     4201 aacctactac cggcctgcat ttcgatgata ttaagatgct cctcaatgtt ttgaatcggt
     4261 tggtagatca aggcaatact gtcatcgtca tcgaacataa tctagacgta ataaaaactg
     4321 ccgattatat cattgattta ggcccagagg gcggtgataa tggcggatat atagtctgcg
     4381 aaggcactcc tgaagaagta tcagcccacc ctgcctcgca aaccgggaaa ttcttagcca
     4441 aaatcctata agctccctga aaagggctcg tttcgtggtg ggataaacac aattcagtgt
     4501 cctattcgcc acgattctag cagaccataa atatttataa ctacttgata atattaatat
     4561 aatcctattg gcacaattat tgtcctctta attagtgttc tttgaaacca tactacccca
     4621 gatggttagg cgtaccctca caatgatgtc gcccactcag gaaaatgccc aaaagtagct
     4681 ggaatgaact ctgcccagat gtttcattcc agcttttctt ttnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnttcaatta attttggaaa aaatctccga ttttatttaa
     4861 gttgccaagc aagacaaaaa ttcgattgga gaaataatgt ttaaagaaaa aatcagacag
     4921 gcaatcgaaa ttctcaaaga acagggagtt gatatgtgga tgacgtttgt ccgcgaaacc
     4981 gaaactttac ctgacccctg tatggatttg atcgttggaa ctaattgtac ctggcaatcg
     5041 gctttcatca ttacagccaa aggacaaacc atcgcaattg ttggtagtct ggataaggaa
     5101 cgcattaaac agactggatt atatgatgat attcggattt acgttgacga tttcggcgcg
     5161 gcttttctga acgcactgaa tgaaattaat cctaatcgaa tcgctatcaa tacctctatc
     5221 aacgattata tggcagatgg cttaacgcat ggtatgtatg aattactgat gggctatact
     5281 aaaaacacgc cttatcgaga gcgttttatt tcttccgaaa aaattgttgc ggcgctacgc
     5341 gggcgaaaat cgcccactga aattcagcga ataaagagag cgatagaaat tactgaaaaa
     5401 atatatgcag aaatcagtcc gcaaattaaa tcaggccgca ccgaaaagga gattgcggat
     5461 attatcttga aaatggtagc tgaacttggg gtacaaactg cctgggaatc gaattcctgt
     5521 ccggcggtat ttaccggacc gctctcggct ggggcgcatg ccgaaccaac cgagcgagtc
     5581 attcagcctg gtcacgtgct caacattgat ttcggtgtga aaaaagatgg ctactgttca
     5641 gatattcagc gcacttggta cattttaaga aataatgaag aaaccgcgcc tgaacctgtt
     5701 caaaaaggct ttgcggtact tctggagtct gtccgtcgag ctgtggatgc catccgaccg
     5761 gggaaaatgg gctatgagat cgatcagatt gcccgcgatt atataactgc caacggctat
     5821 cccgagtttc cacacggcct tggtcatcag gtaggtcgat caactcatga cggtgccggg
     5881 atgttgggac cacgctggga acgctatggc gatacggttt atttaccgct ggaaatcggt
     5941 caagtgttta ctatcgaacc gcgtttgccg atagaaggtt tcggagtggc aacggtcgag
     6001 gaaatgatcg tggttactgc tgacgggtgc gagtatttga gtcatccgca aaaccagtta
     6061 ttttactccc gctgagtgct atactataac ttactttttc ttctcctttt ataacgaaat
     6121 gaaaacttcg accatcccga gttttcggcg ggaattcttt ggaggctttt taaggttaaa
     6181 ttttggccgg tttgattctg aaataagcgg agaaatgata acagttaaat caacaccgga
     6241 cttgtttgat agccaccggc agactaattt tgccttgatt tttgatttta ttttatgata
     6301 gattcgcgaa gtgtctttga taactggtag ataaatataa caaatgacat tttataaaaa
     6361 atataattac ttgatattga taaacatagc ggagttttag tgtgagtgct aaggttaaat
     6421 ctttaaccaa gcggatatcc agctttttct tctccctgcg gatgcagatt gccaacaatc
     6481 cacttaacga atatatccgt ctgctgtttt ttgcctttct gattggggtt ttggctggat
     6541 tggtgactct ggctttttac aaactaatcc acttgataag ctattttcta tacgattatt
     6601 tacctgcaag gtataataat ttgccgaaat ggacgatggc tctttttccg gcggtcggag
     6661 ggctgattgt tggcttaatg attaaactat ggccgcgtca accgcgtcag cgcggagtag
     6721 tagaagtaat caaagccagt ttcatcggac ggggtgtaat ccgagttact actatcattt
     6781 ttaattttat tgcgacggcg atttttctcg gaacaggtgg aagtctggga ccggaagcgc
     6841 cggtcgcatt tctgggagcc ggtagtggtt cattaatcgg tcaagctttt cgagtgtcgg
     6901 aaaatcaaag gcgagttttc ttagccgcgg ggacagccgg tgcgatagcc gctgttttta
     6961 atgctcccat cggtggagtt ttttttgcca tagaaattgt tctatttaat aatttccaga
     7021 cgcttacctt ttcggcttta ataatctcag ccattacagc cgactacatc tcgagggttt
     7081 ttctgggtga tcaactgctt ttgactctgc caaaagttga aatggcttta tccctcaata
     7141 tattatattt tgctgtttta ggtattatca ccggtttatt ttcattggct tttattcgtt
     7201 tctcgggtaa aatgcacaat tttttcgatg ctcagaaagg tcgttttgca ccgttactca
     7261 ggcttaccgg tttaatgcta atattgggca ttgtcgggct tttcttcccc caaattattg
     7321 gcattggcta tgatgccatt caagaaattg ctaataataa ttatacttgg cagattctag
     7381 caattgtatt cgtacttaaa tttttatttt cgggattgtt gtcagccgcg ggtggttttg
     7441 gcggtctatt tgccccttca ctccttatgg gtgcggcgct tggcgcattc ctggctgagg
     7501 gcggcaatca ggtattgggt ctttcgctgg acgtagccgg tttcgccatt gcgggaatgg
     7561 gtggtatgct cgccgccgtc aacagcatcc cggtgacagc attattaatc atcgttgaaa
     7621 taaccggcaa ttatcatctc gttctaccct tgatgctcac cgtcattatg agttatataa
     7681 ttgttcatta tgtgctaaaa gagaccgtct attcgatcga attaagacaa agaggcattc
     7741 aattatttga cggcaaggag ctgaatgtct taaaaaatgt aaagattgat cagattatgt
     7801 cgtatgaatt ccataaagtc tattcaacta tggatgtaaa aactctggtg aaagtcattg
     7861 cggaaggcgc agccaatatc tattgggtag aaaatgaaca gcataagatt atcggcttta
     7921 tctcatggtc ggatttaaaa ccgctcatta ccgattatca aaatttgaat actttggtac
     7981 tcgtctcgga tgtgatgaat cctaatgtag tatcggtcaa aactgacgat actctcgatt
     8041 acgtaatgaa attgtttggg cgttataatg ttgatgaatt gccggtaact gatcccgcca
     8101 ccggaatgtt gatcggaacg gttaaccgta atcgcgtcat ccaattgtat aatcaagaaa
     8161 ttttcaagcg cgagacggcc ggcgatctgg cgactcatat tcgctatctc gatcagaccc
     8221 gttttgtaga aattgatgag gatttttcca ttctggaaat gagcgctccg gaatctatgg
     8281 ttaattccac cctaaaagcg ctcgatctgc gtaaccgtta tcatgctcag attattttaa
     8341 ttaaaaggtt ggataataaa ggccgaagaa tggtcatcac gccccgcgcc gacacgatta
     8401 tcctgccaaa cgatatcttg atactagctg gcccaaccaa aaaattatac gaattaaaag
     8461 agcgctatta tctttgaaaa tgatcctgac gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8581 nnnnnnnnnn nncagaaatc gagcagattt ctgcgctccg taatccggaa ggggttctcg
     8641 ccattgcaaa aattcccagc gaagtaaatt tgctcgcgcc tctcgattat ccggcgctct
     8701 atctctggca gattaatgac ccgggcaatc tgggtacaat cctgcggacg gcactttggt
     8761 tcgatgtaaa aactatcctg ctttctcctg attcagttga tgtatatagt cctaaagttg
     8821 ttcgcggcgc tatgggtgca cttttccggt tgaaggtgcg aacggaggtt gacctgtctc
     8881 ggatcgagat gattttaggg cgggacaatg cccaaatgtg ggcggcagat atgtccggca
     8941 ccatcgagaa acctgaaata acgaatgacc gttttgttat tgccttcgga agtgagtcgc
     9001 acggcttacc gcctgaaata ttacagcgcg ccacgggatt aattcgcatt aataaatacg
     9061 gttatggcga atctttgaat gttgccgtgg cagttggaat cattttaaat aatattcggc
     9121 aaaagtaaga ggatcgaact tggctttcct tggctatgtt ttagcaatta tattgttatc
     9181 ggtaacccca gcctcacccc aggtcaccgt cgatacctta gcaatcggta cggtagcgcc
     9241 gagcttctat gctagtgttc tcgacggttc agattttttt cttagccata aagtcggcgc
     9301 taaagctcat ccgaatgaga aagcaccggt tatcctcgtc ttctttacaa ctttctgtat
     9361 tccctgtcgg caggaaatac ctttcctcga cagcttaaga atcgaatatc cacaaattgc
     9421 cttttatctg gtcaatatcg gtgaagaacc ccagattgtt aaagaatata tacaaagaat
     9481 gaactataaa atgccggttc tgctcgatcg ttatggacag gtggcaaagc gctaccaggc
     9541 tagtatcact ccaactctgg taggcattaa tgtcaatgga caaatcgcct ttctcaagcg
     9601 cggttttcaa ggcagtgaca aagagctaat tcaaaccaat atcaaaaaac taaccgatgc
     9661 taatttgttt attgagaaaa attaggacgc aaattaatga taaataatca ccagtatttt
     9721 gagccggaat tgaaacccca tccaccaata atcgtcccca aaattaaaac agcaattgga
     9781 ctattaatta tggggctaat ctgcgcgaca ctggtttcct taatgataat gatttttgtg
     9841 ccgacgagta atctgacaaa aactactcag ttgttattat tgattgcgga attgttttta
     9901 ccagtcccga ttttcttttg ggcttggcgg aggcacacta atttgggtga actgctaaga
     9961 atcaaacgcg tatcctcacc ttctataatc gcctcgatga ttgtttcggt tagtttgaca
    10021 gtcattatag atgaattgga ccgactcatc cagatcgttt tgccattacc ggagagtttt
    10081 gcgtcaattg gtgagattat gctcataacc gactggcaat cggctctgct gattatcggt
    10141 gtggttgtat ttgccgctcc tttagtcgag gagatgatgt tccgtggttt cttccaacgc
    10201 attttagaat atcgtcaggg tgatataacc aaatcagtct tgctctcagc gatggtattc
    10261 gctctggttc attttaatcc ctggtgggtt gtccagattt acctaatggg actttttttg
    10321 gggtatttag cctggcgggc aaactcgatc tggccttcgt tcgttttaca tgctgttaat
    10381 aacggctggt cggtttggtg gagccaccaa ccggcattcg ctaagtttta cgaatggcat
    10441 ggtcatgtcc atccagtcct aattatcgcc agtttagtgt gtttctattt aggaatgcgg
    10501 ttttttatca aggttacgcc ggtggtagaa aaacgtcagg atatagtctt gatcgaaaac
    10561 ctaagcgatt actatgaaaa cgaacctcct gaataatatc caaattatgt tgcaatgata
    10621 ataattcacc tgattacatt tataagcatt gtaatctatt tagtcagcct catcattttt
    10681 cgttggggac taaatcaccc acgacaacag agaaccgctg atttacccac aatcagcatt
    10741 gtcatcgcgg tcaaaaatga agcagtcaac ctgccccgac tgatcgaaca attgaccgga
    10801 cagaattatc cagatggaaa acttgaaata atcatcgttg ataatgactc aactgatgag
    10861 acttgggcaa ttttacaaaa cgaagccgct agaattagta acctgcggat ctttactacc
    10921 aggaccgaag gaacaccgta tcgtcataaa aaagccgcat tagcggtagg aattcgcgaa
    10981 gcccgcggcg aggtaatcct gactacggat gcagactgtt ccatgggaaa agactgggcg
    11041 cgaacgatgg cgagttattt tactgatgat gtcggaatcg tcgtcggatt ttcggctatt
    11101 cggtatctca atgactggtt ttcgcgcctt caggctttgg attatctgca attgatggcc
    11161 gccgcccaag gctcgaccaa tctcggcttg gcttgggctt gtagtggtca aaactgggct
    11221 tttcgtaaag tactatttga gaatgctggc ggctatatgt tggtccgcga tcgagttggc
    11281 ggcgacgact cactttttat gcaggttatg cagaaacgca ctcgcacgaa agtagtattc
    11341 gccgcggata aagccgcctg ggtggaaacc caagccgctg atagccttac gacttttcta
    11401 cgtcaacgca ttcgctgggc aagcgaagct aattacatgc accatttcaa ttgcctattt
    11461 ttcgtcgtaa ttctagccac atttctggct aatctaatgc cgattgtcta ttttgttctt
    11521 gcaataacgg gagtagttac cttcgttccg ttcttaattg taattgcaat aaaattaatc
    11581 ggcgaagcaa tgttagcttt taaagcgatc cgggtttatc aaagaaaaga cttgatgggt
    11641 gttttcatac cctggtttat catccaacca ttttatatcg ttttaatggg tattttaagc
    11701 ttctttggaa accgtttggg ttggggcgag cgggaaactc ggtctgcctg aaaatcaaaa
    11761 atttatttcc agcgatagat tttcaatgag ccgatcacct gcgataataa atagcagacc
    11821 gggtaaatta cccatagaat cggaaaatat ttgagcaaat ccgtttggtg aaataagtgg
    11881 gcgccgtgat aaacggcgat aaattcagcc ataaatttga aaaacaacat cattcccaac
    11941 caattccaaa gtgacggcga aaaaagcatt atcgccagca cgacatgaaa caggaaaaca
    12001 attactccga gaataaaaaa gggaatagtc agattaaatg ctttactatt gcggcgcaac
    12061 tgttgatgga tatatttttt accaaacttc gttggctctg tggtaacata actggctggg
    12121 tcaatgttat aagcaaaccg ccagcgggtt ttcgtgcgga tttcctgcat tagaaaaaaa
    12181 tcatcgcctg tatcggtatt agcgcttcga taatagccat tgacatcttc aaaagccttt
    12241 ttccggtaag ccagattacg tccgttggca tgcgtcggtt tattataaaa agtcgaagcg
    12301 gcggcaacgg acgcttcaca aagggtatcg aaccgttcat aaatgttcat aaaccctttg
    12361 cgtcttgcga ccgggctatg accgagaacc atgccaactt ctttgcggaa atatgatacc
    12421 atcgaagcaa tccaattcgg cggaactacg caatcagcat cggttactaa aataatttcg
    12481 ccacaactat tctgaacacc cagcgtgata gcgccttttt tacctttgag ttgatcggta
    12541 gttttatcat gatggagaac cttccaattg gattttccgg aagcaaaatc gttcataatg
    12601 cttagagtat catcagtaga atcatcatca accagaatta tttcgtattt gtcttcggga
    12661 tagcgcaaat tggtcaggct ttgcaataac cggctaataa tttgtccttc attgcgggcg
    12721 cagacaatga ctgaaaccgc aagtgtctga aacactcgtt tctgggtgcg aataaatatc
    12781 aagccaattc cgattactcc cattataatt agataaaaaa atgtcagcag gatcgcaaat
    12841 tcaattagat taagcattag aaattgattt cagtttcaat aaatccaaag cggtaggatt
    12901 cttggtcgtt attaagttta gtattataca aattaattcc ggctttaatg aaaaagtcgt
    12961 taaatatctc atagcggccg gcgaccttga gctgattggt tgtctcaata tcacccatca
    13021 gccacttggt ggcgtgatca tattgctcgt cacgtagctc ataactgaca gtggcgtctc
    13081 cgccccaaaa atgagtggaa tcgtcaactc cctttttcag gatcaggtat tcgctggtca
    13141 aggttaagcg ccttgaaagc gctgtcccaa ttttaaaata ccaggtttgg gaattggcgc
    13201 cgtatggaaa tccaaggcaa atgctattat gagtataatt ggtagtcaga gtcttatggg
    13261 aataagtcca ggggcgcacg gcggtaaatt ctgcctgaaa atcaaaaggc aaggtggcaa
    13321 tgttaggcga ccagcgcatg cccgcctgcc aagcgtgctt attagcccac cattgtttac
    13381 caagttcact aaaacgcatt tcgtcaatta gaaccgagcc atagaacgtg atatttctga
    13441 taaaggtcgt tttaacatcc atcgccatta gagtattgtc gcggtcttcg agagcgtgcc
    13501 cgatcggcca ataaaatcca aaaggtgtca tataggctat atcaatttct cgatcaccat
    13561 aaatcaccat ctcgctaaac ccgacagtag tcgttccgcg gaaaaaagga atgtcgagac
    13621 gatgggcggc gatatacttg gaacgatttc tctgctgaaa tgcaatatcg cgataatggg
    13681 tgctgtcgtt caatagcgat ccatgaatca tagacagctt tatccctttg aatttggtat
    13741 cgaacccaat ataagaaaac agcggcgcac tttttgataa aatcaagttg ttagtcccac
    13801 ttgctcccca ggcaaccggc tgacggtata agccgatatt gaagtatttt tgatgaaaaa
    13861 caagagaagt ctgcaaaata tcaaaggtga ccgtcccgcc gggttgtacc atactgaagt
    13921 tgccgatttc ctcctcatag ggttccgtaa acgccggatt accatttttc ataaagcggg
    13981 caaagtaggt gtgaaaagtg aacgacttcc ccagcgcccc tcgtaataga tagcgatcgg
    14041 aaaacatttg cttctggtga ttttctttcc aatccatccg accacgcgct ccgacgtcaa
    14101 accagaaaaa ggtctcgcca ctttcataca tcaatagatg attttcctct accgcatctt
    14161 gtcgcgaaaa cagattggcc gttgcctgcc gaaaatagtt tttttgataa aatggggctg
    14221 agatcgaatc cggttcggtc aattggcgtt cggtttcata gcgataatcg gcaagaaatt
    14281 cgtgtaaaat tttcttttcg atggctgaaa gttcatcagc tttttgggag gcgatttgca
    14341 ggaaattaac gatttcatca cgggttaacg gtaaagtcag atcgttaaat cccttgatga
    14401 cgccccgcgt ttcgaggcgt ttcaaaaaat catagaccgg atcggtagct gggacgtcaa
    14461 taccaatagc agaaactacc agcggaaaag cgataatgat aataaaagtt aggtttacaa
    14521 accgaatgtt tctcatcatt ggtacttaaa tatacaatta ttttatcagt agcgaaaatg
    14581 cttaatgggt tcgcccatcc ttccaatatt agtcatagcg tctataccga tttgtaagtg
    14641 aacctgactc cattgcgccg taacttgctt atctaactct tcagttttaa taccttcggg
    14701 agtcgttggt aggtctgaaa cgaggagtaa agcgccgcgc gcgatctcgt tataatgtcc
    14761 gacgataaaa agggtggcgg tttccatatc aatagcgata caagtcattt cgcgtaagcg
    14821 ttggtggaac gcctgatcat gttcccaaac tcgacggttg gttgtataaa ctacaccggt
    14881 gcggtactca agcccgcgct ggactataat ttctgagaca aatttatgca atttgaacga
    14941 tggcaaagcc ggaacttggg gtggaaaata gtcgttgctg gtaccctctc cgcgaatagc
    15001 ggcgattggc aaaataaaat gtccaatttc agttgatttc ttcaagccgc cgcactttcc
    15061 taaaaacagc actcctttgg gatgaaccgc caccagcaaa tccataatag tggcggcatt
    15121 cggcgaaccg attccggaat tgataatagt caaaccagct gaattcgtgg cggcttgcat
    15181 tggacgattt tcaccgtaca aatcacattt gaacatcgca caaaaaatgt ccacataatt
    15241 gcggaaattg gtcagcagaa tataatcgcc gtatttttcg aggggcagac cagtataacg
    15301 tggcaaccag ctccgagcaa tttcgatttt ttccttcata actacacctt cttcaaattg
    15361 aaaataaaat cagagccatt aaagcacaga acacacctaa accctgtctg aaactaaacg
    15421 gttcttttaa aaagattaac gccagaacaa tactaatcaa tgggtagaga gccgtcatcg
    15481 tcacaatggt agtaaccttg ccacgactga cagcctggag aaaaaacagc gaacccagca
    15541 taccaaacag accggttaaa attgcccaga acgcgccaac tgcctgccat tgaatttggc
    15601 tcctaaccga tagtaaaacc cccaaaccaa cgattgtccc accaatggct tgatagacga
    15661 gtgcgctcga cggatcaatg tagcgagtgg ctaccttcgg taaaaagccc caaaatcccc
    15721 ataagattaa agcaattagt gccggtaaag tccaactcct caagtcaatt ctccttgtga
    15781 ataattcgat taaactatca aatttgctga gcaaagtaca aaaattcttc tggccagtca
    15841 aatttatcta atctaattat agatatgaag atatatgtgg atagtgatta aaaacatcaa
    15901 tgatcacaga cattgctggt cagttgcaga tttccatcga aataatcacc gattaacttt
    15961 tccggtgatt cagaacaggc gcccacgagc acctgaatat tattttcggc gaaaagcatc
    16021 agcgcgcgct gtcccatacc gccagcgata attagattta cgccgtgctc cgccagccag
    16081 ctaggtagta aacccggctc gtgcggcggc ggtataagat cggtgcgttt gattattttc
    16141 ttattctgaa cttcaacatc aaccaacgca aattgttcgc aatgtccgaa gtgcagggtt
    16201 agtttgccgt tggcgagagg aatggctatt ttcatttttt tattctcctg tgattaagac
    16261 ttaacatttt cggaatccat atccagaatt ggcttgatga tttgccgcat gatttccgca
    16321 gttgggcttt tactatattg gtgaataaag acctgaccgc tgtcgccggt ttggccaatt
    16381 ttcggatcaa tcggaataga acccagaaag ggaacctgca tactttcggc aatcttcttt
    16441 ccgccaccgg taccgaaaat cggcgtgact tcgccgcatt tcggacaaac gaaactgctc
    16501 atattttcca caataccgag tactttgaca ttcaaatgac gacaaaatgt aattgatttg
    16561 cgcacatcca ctgcggcgag tttttgcgga gtagtgacga caattgcgcc atccaggtcg
    16621 ccgattagtt gacaaaccga aagcggttcg tcgccggttc cgggcggcga gtcaataatc
    16681 agaaaatcaa gctcgcccca gctgacatcc tttaaaaatt gcttaataat acccattttc
    16741 aaaggtccgc gccagataat ggcatcatcc ggatttctaa gcatgaagcc aatcgacata
    16801 acttttaggt cgcccagctc aatcggtatc atcccgtcat catctttctg aattacttca
    16861 tcttccagcc ccagcatagt cggaatgctt ggtccgtgaa tatccacatc cagcaaaccg
    16921 actctcttac ctgccagcat cagagccacg gccagattaa ctgccaccgt gcttttaccg
    16981 acgccgcctt tgccggacaa cacgataatc ttgtgccgga tgtgacaaag ccgggaaagt
    17041 aattgctggc gctctttgaa ttcctgctga ttttcacctt cccgaggctt tgccgccgaa
    17101 caggtcgaat taccacagga atcgcaggta ttttttgttt tttctttact aatatcactc
    17161 atatttcaat ctcctgtaag acctaattta ttccagacat tctttatatc cattgcgcag
    17221 ggcgaatcag tttctacgac tgataaggct tgtaattgcg ctttcgtcac actcaaatca
    17281 taacgtatcc gaccgacagt tttggcggaa ctctttcggg cttttttttc aatttgctca
    17341 gtcattccag gattaagatc ccacttgttg acacatactg aagttggaat attgaagtgg
    17401 cgcgtcagag ccagcacgcg ttccagatcg tgttcgccgg taactgtcgg ttctgtcacc
    17461 acgagcacct gggtcgcgcc agtaatcgaa gcgatgaccg gacaaccgat tcctggtggt
    17521 ccgtcaacaa taactaaagc gcgcttttca gattcggcaa gtcggcgggc agattggcgt
    17581 accgttgaga ccaatttgcc ggagttttca gccgcgatac tgagcttggc gtgcactaac
    17641 ggaccaacgc gcgttgccga aacaaaccat tcgccgcaga gacgatccgg aaagtcgatc
    17701 gccttcaccg ggcaaaattg cacacagacg ccgcaacctt cgcaggcgat cggatcgacg
    17761 aagaatttat ttccggctga cgcttcttgg cgggtcagga ttgcttcaaa gcgacaatgc
    17821 gccaggcagt cgccacaggc aatacaatcg gcttgacgga taacggcttc atgtccggca
    17881 aaaaaatcat ggcgttcttt caactgcggc gaaagaacca ggtgtaagtc ggcggcgtcc
    17941 acatcgcaat cggctactac cgcacttttg gctaaaaccg ccagtgccgc cgcgatgctg
    18001 gttttacctg taccgccttt tccactgatg ataactaatt cctgcaatgc cggattcctc
    18061 agatagtaac aattttatcc gaatcgcgga cgatctgata ttgatctttt agagtggaaa
    18121 gcggacagac ttcggaacca tcggagttgc gtagttttag acaggctccg caagccagga
    18181 actgaccgcc cttgtcaatt accttttgag ccagcgtttt gacatcgaat tttgaatctt
    18241 caatttgatc gatttcgacg cctttaccgg aaaggaaaat gcgcacttcg tcaccctgtt
    18301 caaggctata gagtgccaga cgcaatccgt taaaaacggt ctcggcgtca gtctgagtaa
    18361 taatcattcc gagtttcatt ttgcatttca gcctttcatt tacacttttt tgctctcatt
    18421 tatatttcta tttgattgcc ggcgccggct ggaacataat tcgcaccgaa tcttctttta
    18481 aaaattgcct gtgcaggctc gccggtacaa tgggctggac taactttctg cactcctact
    18541 gtgaccaatt gagaagcaat ttcttcaatc gcctcgcatg cgctatctcg caaatgaaat
    18601 ccgcccagga ttaatgcaat tttttcagcg ggaaaaattg tcttcacttt cgtaagaatt
    18661 gtaatgattc ccggatgaga acagccggtt accactgcga tcccgcgcgc cgttcgggca
    18721 atcaaggctt gctccggctt cggtgaaccc ttatattggg cgggaatttc acccgttaca
    18781 ccaatgtgat cgtcaatttt cctgaaaccg atcgattcga tcagcttacc ccgtaaagcg
    18841 tttacccttt gcttaaaatc attgccaaat ccgggacagg catatacttt taagcccggc
    18901 tgtcgttcta ataatcccca taatccgccg gtatgatcgt ggtggtcatg tgaaatcacg
    18961 acagcttcta ttgtcttagg atttattttt aactgtgcca aattttcaca aagcggctcg
    19021 tacttatcgc cggtatcgaa gagaatacgt ttatctacca gacaggcaaa tccccagcca
    19081 cagcggattg ccgggctcaa cgatgaatta tcataaagca ctaagaattg cattttccgt
    19141 ctccggcaac cttgttcact tgcggatctc atcgtttaaa gattttatgc gtcgggtata
    19201 aaatgttttc ccccgatatt ctttttttac caaaagctct ttttcaatta aactatcaac
    19261 gaccgaccag tcagatttag ctttttccaa taatttggca atagcctctt cgcgcatcgg
    19321 atggacagcg gtaatattca acaaatccgc ttccacattg ccgctaaaag caaaatcgtt
    19381 tccttcatat tcaattatta atttcacatt ccggatttta gagctgaaaa tataataagc
    19441 ctgaatcagt atttcttcgg tcggagcttg gacccatttt tgggaaggtg ggcgaatagg
    19501 aatggaaaga taggcggtga tatccggcaa ctgggcgata aattccacca gccggtcgat
    19561 atgcgcctga ctgctgttat gatcgcggat cagcatcgtt tcggtaacaa gttgaccttg
    19621 ataatgcctg gcgaattgca gtatgccatc gagaattttc tgaagatcaa gcgaccggtt
    19681 cggacgatta atcctgtgcc agatcgcttc atcaaccgta tcaattttca gcgaaaccca
    19741 gtcagcttta ttcatttctt ctcttacgtc tgtacgccag atcagcgaac tgttggtaat
    19801 cacaccgacc ggaactccca aggttttcaa catactgatc tcccgaccaa ggttaatgtc
    19861 cagcgtaggt tcgccatccg gaacaaaagt caggaaatca acctgttcgc ctttgtgccg
    19921 ggcattgtcc aaaagatatt tcacgttcct gaaaatgtct tccggtttgt aaaaagcctg
    19981 acgttcaacc ggcattttgt cggtttttcc gacctgacag taagcgcagg cataagtgca
    20041 tattttggcg gggatattgt tgattccgag gcttttcccc agccgtcgcg atgggacggg
    20101 accgtaggca tattgtaaat cgataaggtt caattattct ataccatcat tttgagtcaa
    20161 aacgataatt tcttgcatgt gaccgcttag gcagttagtt gttttaaatc cttttgtatt
    20221 taagaatctt tccgccgttt tcagtgtctc tccgctgacc gaatgcagat gaccatcttc
    20281 gcgatgtaag cgcgcaacga tttcaaaacc ttctgcagaa aaatccgcta tgatcataat
    20341 ccctcgcggc gccagaactc gggcggcttc ttccaatacc ggcgcggcat tctccagatg
    20401 atgcagaata tttgaaagaa caacacatct aaaataatta tcgggataag gcaattcggc
    20461 ggcattttgg ccgataaatt tgattcggtg ctcaaggccg gcttctctgg ctaaaaacaa
    20521 cgcaaaggct tgttcatcgg tctcaatatc aactgaaatt acatccaatc cttttttcgc
    20581 cagcgccata gccgtcaacc ccttgccggt accgatatcc agcgccggac tggaaagatc
    20641 gccacgggtg gcgatcacga aatcggaagc cgcaaagcga tcatagccca aacgcaggta
    20701 ttgggcggtg cgggactgga aagatttctg tttttcaagc aatttgctct gtttatttct
    20761 attttctgaa aaagcgctca tatattttaa tcacttcggg ttgtaatgtc atttataatt
    20821 ttttcaaata ttaggcgata ttcgggaaaa acctttccta tcagttcgcc gcgcgaatag
    20881 gcttcggcaa tctttcgatc atccggaatc tccgccagaa tcgggatatt ttcatcggcg
    20941 cagtaaaaat ttacccgcgc atcgccactt ccggaacggt taataatgac cccgaagggt
    21001 agatccattt cgcggacggt ttcaaccgcc agaatcagat catttaatcc aaaaggtgtc
    21061 ggctcggtca caagcgtgac aaaatcggct ccccggagcg tcgtaattac cgggcaggaa
    21121 gttcccggcg gcgcatccaa taatatcgga atacgattgt ccgtttttcg tttcacagcc
    21181 cgaattaacg gcggagccat tgccgcgcca atatccagat taccctggat cagagtaatt
    21241 ttcccggcac taaatgtctc aatgacgcca atacgatagt cgacttcacg gatcgcttgg
    21301 ggcggacaga ctttcataca cccgccgcat ccatgacaca tatctgcaaa tacaagcggc
    21361 attgtaccat aggatacaat ggcattatat tcgcaaaaac gactacattc gccgcaacca
    21421 ttacaaagac ttgcatcgat ctgaggaatc ggaattgtca cttcttcagt agagagcaga
    21481 gcaccattca gaaacaattg ggcattcggt tcctcgacgt cacaatccag aagctgtact
    21541 tcacggtcca gggcataggc caggttgacg gcaatggtcg tcttgccggt tccaccctta
    21601 ccggacgcaa ccgcgattat caatgaatgc tcctgttttc tggctgaatt tcattggatt
    21661 ttagcaaaac ctgggttccg accggatgac caatatgcct ggcgattacc ggacatttta
    21721 ttttgaacag gaaaaaaata ttgcgcggtt cgttgcaaag agtctcataa ttgccttgac
    21781 ccttggcaat gatcagatcg gcagtcgcaa agcaccttag aaattcacga ctgcaatcac
    21841 caagcagagt tccaggcgca tctgagccgt tatcaataat atcgacaagt ttatccagac
    21901 cggcggcttt tgcgtcagtt tgcaaagcat cgttgatagt tggagcgcca cgcactacca
    21961 gtgtcaccgg tttaggtaaa atctgctcga tcagcagacg atcaaaaact atttcgccgg
    22021 cattgtctgc caagtataga atatcgcggg cttcgctaac cgccgattta aatttttccc
    22081 aatcgccgac gagctgttca ttcagagcct gatcgactgc ctcgtgaata tccgcgattg
    22141 tcagattgcc gtttgcgccg agatctataa tatttccggc aattgccagg cgcgccgcca
    22201 gcaaaagcgg atcgggcgaa atgccgatct gttcctttaa cgctggcagt aatttgagtg
    22261 caagccggtt ctgtctatcc ttatcagcct ggtataaatc cggaatgccg gtaatttcac
    22321 tcaggcgacg gtaaaatatt tgactggcgc gcggtggcgg ctcgcgaaaa tcgacggcac
    22381 cgatacagca gagagcttct cttacaattc gttcttgaac atctggatca tcagataaat
    22441 gccgcgcggc ccggatagcc tgctgtacaa aacaaggaat acagtcgaga tccatttgca
    22501 ttatttccgg cgccctctaa tctgagcggt ttgttcatcg atctcttcct gccagccgtc
    22561 acgactatta tcgatccggt caaagcgctt tatatacggt ttaatgtcga gcaaaggagt
    22621 accgtccaaa atatctgctc ctttgatatg caaaatattg ccctcgcgtc tgatcagctc
    22681 gacaatgctc aatccgatcg cgttaggtcg gttgggagac cgtgtcgcaa atacgccgcg
    22741 ctcgacgtct tgcagaaaag gcttcacccg cagtttagcc ggtttagccc gatgaaaatg
    22801 atatatcagg taaatatgcg agaagccatc cagatcacgc agtccttcgg caaatttctg
    22861 atagacttca acttttgccg ggcaatcttt ggcgtaaacc ggctgaattg gtatctgttc
    22921 cgcgttttga tgctggctgt gaattatccc gatcggtcgg aatgaaactg aggcaattct
    22981 catatccaat gtccggcgac atcttcgctc ttagattcgc taagcttgcc tgcccggtaa
    23041 tccgccaggg cggacgcgac cgttgccgca cttgtggtat aaatcttgac accggcggat
    23101 tgcaaaaccc gaaaagcctt tggcccacaa tgggcggtga ccagcgcttt ggcgccgctt
    23161 cggactatgg tttccgctga ttgaatccca gcaccctgag cggcattcag gttttgccgg
    23221 ttatcgatta tctcgaatgt atcattttca aggtcataaa tcaggaactt ctctgctcgc
    23281 ccgaaacgat tatcgagtgg tgcatttagg tcattgcctg aagtcgtaaa tgcaattttc
    23341 ataatacaca ccccttaatt caaggttgcg tgagcctgat aacccacgca acctcgcagt
    23401 tttaatttcc ggaatcgcca attacttggt aatggctttc cagacgtctt ccttggtgat
    23461 ctccttcaga cagaagaacc aatcgagaca cttaatgccc tctttgctct ccatacgttc
    23521 tttggcaacg ccgcaggagc cagcggtcgg aacgatgaca tcatcaccag gacgccaatc
    23581 agccggagtt gaaacgttga aagcatcagc tgtctgcaga gcaattacta cccgcagcaa
    23641 ttcgtcgaaa ttacgtccca gacttaacgg gtaataaatg attgtgcgga taatgccttt
    23701 aggatcgatt acgaagacgg cgcgcacggc tttagtattg ctttcacccg gttgaatcat
    23761 gccgtatttc ttggcaactt ccatcgttat gtcttcgatc aatggaaatt tgacctcgac
    23821 atttttcata cctttgtagt caattttttc cttaatggtg cggagccagg cgatgtggct
    23881 gtatagaccg tcaaccgaaa gtccaacgag tttgcagttc acttcggcga attgtttctc
    23941 catagtggca aaggtcataa attccgaagt gcagaccggc gtgaaatcag ccgggtggct
    24001 aaataatatt acccagcttc ccctataatc ctgcgggaaa ttaatttcac cttgagtagt
    24061 tatagctttg aaagccggag ccggatcgcc gatacggggc attgatacgc tctgagtctc
    24121 attaataggt tccatgtttt tttcctttta tggtttgttt atatcgcttt ttaaataagt
    24181 cggtctgaat accctaaaaa acataccgga tcaacatatt tgctaaacaa acggtatgtt
    24241 tctaactggc ttatttagaa tgcttttcta ctaacatttc aaaatcttcg agttcggctt
    24301 ggagatcgtc ttcatgttcg atctcatctt ccagaatctc cagaaccata ttataggtga
    24361 ccggatcttt accagcggta aggtcgaata attttttata aaccgttata gcgcactgtt
    24421 cgcccttgat attctgttca aggatttctt taacatgcgg atcatccggt gcatcgtaac
    24481 cacaattggt gtgtttgtac caatcctcgg gtttgatcaa tggagtccca cccagctgga
    24541 ttatacgttt cacgagcatg tcggcatggc gcaattcatc agccgcgtgc tcttccagct
    24601 cggcgataac ggcacccttc atcggtcctt tgacaacttt cgcgccgacc cagtactgat
    24661 aataagccaa ccattcatcg gcatatgccc gattcagcag gtcgacaaga tgcttcacat
    24721 ccaaattaag aatttcaatt gcttttgtac ccataatttt ctccttttat ttttattttc
    24781 attagagttt ccgaagccac attggttacc aaactgctgt gacttcggaa atattctctt
    24841 taacgcctct tgtcatttat ccgttttaag cgcatcgaga cgttttttta cttcatttaa
    24901 ctctgcttct aaatactcag cctgagtctg gagagattgc ctttccactt ccggatcaag
    24961 tttttgatac ggggctggat atgcgtaggc aggattaata ttccccaatc ttgcccacct
    25021 cggcaatccg gtcgcataaa accaatgtcg gaatccccga cctcggccgc gtcccattcc
    25081 gccgccgaaa ccgcggccga tatacggatt agcataaccc ggcacattgt ttcctgcaca
    25141 gaatccagcc gcgcgtccag tcattggtcc cattcctgtg ggaccagttc catcacctct
    25201 tggcatagtt tttttctcct tatttttatt gttgtcattt attctctcgc catagccgca
    25261 ccacactgtg gacacttttg ttcaaaacag ggcattccac gttgatgcgg ctctttgtgc
    25321 ccacattccg gacaaacaca attaccactt ggaccagcgg ctgaacctcc gcccatacga
    25381 ccaaagcgct gaccaccacg cccttgacca cttcccattc cacgtctttt cccactcata
    25441 ataaacatct cctggtaatt agtatgaaaa ccaccttgcc accgacctct tctaccacaa
    25501 caacccggca tggcaaaatg atgccaatca gggcgtccgc tgatccaggc ttgaacaact
    25561 tcatccagct cgccagctat aaatggaatc acatcaatac cgttagctac aatcgtatca
    25621 tataacggcc tggaaatggc accgcaaatc agtgtcttaa cgcccagctc tctcaagcgt
    25681 tctgcttttt taattggcaa tagatcaatc ggtaaaattt cttcaaccga tctatttatc
    25741 acactgtttt cggcttctac gatatgcagt aatcttgcca cgtcaaaaac gggtgcgatg
    25801 cgattattcc agatagcaaa ggcgactttc attattttct tttccaacta aacatttcac
    25861 taatgcgagg tgcatttttc gtgccacaat ccggatactg taattattcc actacaacaa
    25921 caataaatac aatatgtaaa gaatttcttg tgctgggcta actgtactcg cacagtagcc
    25981 atattgcaac tcaatcgtaa tagtacagta gcgtatacgc tactttttag ggtgcgatcg
    26041 accgtctttt cttggtaaag aaagaccgag cttcttcatg cggcggaaca gggtggtttt
    26101 atgaataccg agatccttag cagcggcggt gcgattaaaa ttatgacggg ctaacgcaga
    26161 ttggatggtt tgagcctcaa gggtctggac tgcggagcgg atactttcag gtggcaaatc
    26221 agtgacgcgg cgggcggtca gttcttccgg cagatgcttc agtgtaattt cgccatcatt
    26281 gcagagcaca aaagcgcgtt cgatgacgtt ttccagttca cgaatgtttc ctggccagtc
    26341 gtgcgccatc aataacgaca atgcttctat actaactcca cttacggatt tacgccaaag
    26401 cagattgaaa cgctcaacca gttgttctat aatcacgggg atatcctctt ttcggcgacg
    26461 caggggcggc aggtcaatcc gcacaacatt tatgcgatag tagaggtctt cgcggaaggc
    26521 gcctgtcttg accatttcgg ttaaatcctg attggtagct actatgatcc tgacattagc
    26581 agtttcagag cgggtagcgc cgagcggttc ataaattcga tcctgcagaa cgcgcagaag
    26641 tttgacctgt agcgccggac tgacttcccc gatttcatcg aagaacaacg tgccgccttt
    26701 ggctaaggca aagcgtccgg gtttatcttg aacagcgccg gtaaatgcac cttttttata
    26761 accaaataat tccgactcca acaactcgtt cggcagagcg gcacaattga cagctataaa
    26821 aggccctcgg gagcgtggac ttaattggtg aatagtgcgg gcaaccaatt ccttgccagt
    26881 accggtctca ccggttatta acacggtgct agggctggcg gcgatggcgg gcaatacctc
    26941 gaatattttt tgcattagtg gactacgact gaccaaatct ccgaaaccca ttttgttgcg
    27001 cagttcttgt cgtaaggttt cgatttcact aagatcccga aaggtttcgg cgccaccgat
    27061 aattttgccg ttcttatctt taaacagtgc cgtggaaatg ctgattggaa tgcgggctcc
    27121 gtccgccttg ataatatagg cggatttccc aatcacagct ttaccggttt tcagagtttt
    27181 ttgcatggcg caatcggcgc cgcacaaact tgatcgaaac acctccgaac agcgttgacc
    27241 gattgcctcc ttacgcttca cacccgtaat ttcttccgcc gcacgattga atgaggtaat
    27301 gcaccagttc tcatccaccg tgaaaacgcc atcagagatg ctttccagga tattttcgac
    27361 caatgcggat tgaagtgtca tttcatatca aagttaagaa atattttaac acagaacact
    27421 cagagaacgc gacggttatt taaacaaatc ctctcgcctt cagagcgttg cttttgttat
    27481 tttcacaaat tgttttcaat ttttattaaa actcgataag ctctgcactc tttgtgacga
    27541 gtttaaactt tacgctctgc cacccattgt caacgccatc acagctttag cggcgtgcag
    27601 gcggttttcg gcttcgtcat aaattatcgc cgccggtcca tcaataacct cgtcggtgac
    27661 cttcttgccg cggtcggctg gcagggcatg catttatttt acttatgggg cggttaattt
    27721 catgcggcgg gcgatgacct tttcttcttg gccgctcagc gaagctacgg aagccagttc
    27781 tgccagaaag taggcggttt gggaactgat tgctcgggct ttgaatggaa ttttattgct
    27841 cacttcgcat ccttataaaa attatttctg aagaatagca gatgatattc gagagcattt
    27901 gtccagtaat cccaagtatg agcgccgggg cgttcgatat atacatgtgg aatcttcagg
    27961 tctagtaatt ttttatgtag aagaaaatta gtttgatgca aaaaatcatc aactccgcaa
    28021 tcaattaaaa ttgccttatc gtgtccgacc aagcgctcgg ctaaaaataa agccgaattc
    28081 gcttcccacc tttgctggtt cgtttcccaa tcaccgagga catcggccag ccctgcccgg
    28141 cgctcgccgc tggacatcgt cattgcgccg ctcatacagc ctgccccggc aaaacgttcc
    28201 ggaaatttag ccagtaaggt gaaagcaccg tgtcctccca tactcagtcc acaaagaaaa
    28261 cgcccggtgg aatcagctac gcatcggaaa tttgcatcaa taaaatcaac tacctcttta
    28321 gcaatgtaag tctcatactg tgagtctttc acaatcgggc tattcacata ccaaccggca
    28381 tagccgccat ccgggcagac aataatgaaa ccgtacttgt cggctaacgg cttcaaatcc
    28441 attttagtcg gccaatcgcg atatgagcca ctccagccat gtaacaaata tacaactgga
    28501 aaacgatcat tcgatttgtg gtatacatct ggcaaaacga ccacggcttt agggttctta
    28561 gacatagcca cactcggtat taccaaagta tccacctggg cagataaaaa gttaccagcc
    28621 ccgaatagga agacaattgt ccattcgcag ataatcgttg gtattttgct ctttatgtta
    28681 aatttcatta ttacctctct gtttgattat caaatatctt tattatttcg gaagtctcca
    28741 atagttttca tcgaatttca ggattgagct cacgaccaat tggctgactt tcctggtcta
    28801 aaataacttc ggatggtcag ctgtaaaaga tatgttggtc aaaataataa aaattatttc
    28861 cctcttaatt acgattccgt cgcattcagc aatgagaaaa cgccaaaaat aagaagaacg
    28921 gctctactta aaaaaagaac cgcagaccaa aatctgcggc tcttcattta tctatattga
    28981 aaaaacgttt tacttcgttt tttcttcggc aacggcttgg gcggctgcca gtcgagcaat
    29041 cggcactctg aacggtgagc aggaaacata atccattcca actcgatgac aaaatttgac
    29101 ggatctcggt tcgccgccat gttcaccaca aataccgatt tccaggttcg gcttggtctt
    29161 gcgaccacgt tcgataccaa tttggattaa ttcgccaatg ccgacctgat ctatggtctg
    29221 aaaaggatct tccggtagga ttttcttctc gatataatcc ggcacaaaac tgccaatatc
    29281 atcacgcgaa aatccaaatc ccatctgagt aagatcgtta gtcccgaatg agaagaagtc
    29341 ggtgaccccg gcgatttcat tggcaactaa gcaggcacgt ggaatttcga tcattgtacc
    29401 gaataaatat ttgatttctt tcaggccgtg cttcttgagt acttcctgat aaacttcatc
    29461 tacaattgcc ttttggtgct cgagctcttt tgttaaactg accactggaa tcattatttc
    29521 cggataaggt ttcttgcctt ctttcaataa ttcggcggca gcttccaaga ttgctctaat
    29581 ctgcattcgg gaaacttctg gataggtcac tcccagtcga acaccgcgat gtcccatcat
    29641 tgggttagat tcatgcagtt cagcggcacg cttttccagt tcatctaagg aaatattcaa
    29701 atctctggca agtttttcct gcttttcttt ttctcggggg acaaattcat gaagcggcgg
    29761 atcgagcaaa cggataacca ccgggtagcc gtccatcgcc tccagagtcg cctttatatc
    29821 ctttttaaca tagggaaaga gttcatcaag agccttttgg cgctcatcta atgtattaga
    29881 tacaatcatt ttacgcaggg caaagagtgg cgcgtcgcta ccttcgccgt agaacatatg
    29941 ttccgtacga aatagcccga ttccttctgc cccgaattga cgcgcgcgtt tagcatcggc
    30001 tggagtatcg gcattcgtac ggataccgag acgacggaca gagtcacaga ctttaagaaa
    30061 ttttaccaga tcaggatttt cggtagcgtc aatcatcggt agagttccgg catatacata
    30121 acccttagtc ccattcagag taataaaatc gccttcctta aaaacgcgat caccgatttt
    30181 cattactttt ttctgataat ctatatgaat agcactgcaa ccgacgatac aacatttgcc
    30241 ccatccgcga gcaacaagcg ccgcatggct ggtcatacca ccgcgagcag ttaaaattgc
    30301 ctgtgaggcg cgcataccgt ccacatcttc aggattggtt tcctcgcgta ccaaaattac
    30361 ttttttgcct gctttggccc attcgacggc atcggctgaa gagaatacaa tttgaccagt
    30421 ggcggcaccg ggaccagccg gcaaaccttt ggcaagcggt tctatttttt tctcgtgttt
    30481 cggatcaata atagggtgca gtaattcatc gagttgagaa ggcgaaactc gcaagaccgc
    30541 ttcttctttg gtgatgagtt tttcattcaa catatctaat gccatccgta cggcggcggg
    30601 accattacgc ttaccgatgc ggcattggag cataaaaagg cgcccctttt caatggtaaa
    30661 ttcgatgtcc tgcatatcgc ggtagtgttt ttcgaggcga ttccggtatt ctacgagctc
    30721 tttataaata gccggcatgt actcttccag agtcacaagg tctttattat gttcagaagc
    30781 cgaatattta tttattggtg ctggggtgcg aatgccggct actacatctt cgccctgagc
    30841 atttattaaa tattctccat aaaattggtt ttctccattt ccggggttac ggctaaaagc
    30901 tacgcctgta gcacagtcgt cgcccatatt accaaagacc attgcctgga cagtcacagc
    30961 ggtgccccaa tcgtccggaa tgccttcaat acgacgatag gcaaccgccc gtttgccgtt
    31021 ccaggaagca aacacggctc cgataccacc ccataactgt tcatacggat cgtccggaaa
    31081 atctttacca agcactttct taacctgttc tttaaacacc cgacatagct cttttagatc
    31141 atcggcggta agatcagtgt cattagtgta attttttgcc tttttaaaat cgtccataat
    31201 tttttctagc tgacgacgaa ttccctcatc gtctttcggc tcgatcccgg cggctttttc
    31261 cataactaca tcggaataca tcataatcaa acggcgatag gcatcgtaaa cgaagcgttc
    31321 attgccgccg ctttgcttga ttaagccggg tatggttttt tcggttaaac caatgttcaa
    31381 aacggtttcc atcatgccgg gcatagattt gcgagcgccg gagcggactg ataccagtaa
    31441 gggattatct gggtcaccga atttacggcc catgattttt tccactttgg ctagagccgc
    31501 ttcgacttgc tccttcagtt ccgccggata cttgcgatta tgttgataat aataggtgca
    31561 gacttcggtt gtgattgtaa aaccgggcgg caccggcaag cgcagatcgg gatgaccagc
    31621 catttcagcc aagttagcgc cttttcctcc caacaattcc ttcatacttt catttccatc
    31681 ggctttacca ccgccgaaaa agtagacata tttaggggtt ttagattttt gagttgtcat
    31741 ttttttacct cttcatgtta ttctattaac tgtaaaaaac cagcgctaag tttacgccga
    31801 attgactttt ttttcaagtt ttagttatac cgagtagatt aatctt
//
LOCUS       DAOOMU010000015        30360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1309_length_30360_cov_19.010064, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000015 DAOOMU010000000
VERSION     DAOOMU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 30360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30360
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1309_length_30360_cov_19.010064"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(423..1010)
                     /locus_tag="PLI55_02590"
     CDS             complement(423..1010)
                     /locus_tag="PLI55_02590"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPA99756.1"
                     /translation="MIYQTVILAIVAYFLGSISFSYIFTKVRTGKDIRTVGVKNSGAL
                     NVFENVGKGIGLLAGIADALKAVVVVVIGKLIGLDTFPTIFAASFAVIGHCFPIYYKF
                     YGGRGASAALGILLCFIPLEVLISCIPAALIAYCIRRMGFTPVFILGFSPIVAAIFKK
                     PATIIWGVVYLVLLTGVLNLIINISKRDEKLIPEN"
     gene            complement(1023..1904)
                     /locus_tag="PLI55_02595"
     CDS             complement(1023..1904)
                     /locus_tag="PLI55_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPA99757.1"
                     /translation="MDQKILILTGDNLGLKNEQIDYPDVAVVKFPVVIDEKEYFEDET
                     HTAQYLIERFKKEKIVARSQALLRKDIVEVIEKNKDKYDLIVHVLMGSNMSSATVQMA
                     EGIRKDYEGIIPIINIDTKQASSGVGAVLLRVIELLKESDSVDEFVSRIDEVIKNTYS
                     YFCVPDLNYLYRGGRIGRAQSLLGSVLRIIPVVGLFGDETDSGFLPVGKGRTYQAANK
                     IIINHIKQKMVLKKVAKIELINILHFENSDADELKDLEKQINDNLTYRRLVHGHARFV
                     EAVHGGPGTWGGSFVLK"
     gene            complement(1916..>3640)
                     /locus_tag="PLI55_02600"
     CDS             complement(1916..>3640)
                     /locus_tag="PLI55_02600"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPA99758.1"
                     /translation="INDLNVFPVPDGDTGLNMVLTVQGGLAHAKNYASDQSTLSEYLT
                     ALAEGMTLNSRGCSGVILALFIQGVAKKLADWPPDQKIPNTEIAQALISGYKYAYAET
                     ANPKEGTMLTMMRVFAERFSELVMDGKDALSIIKDIVPDLEETLEKTPEMLPVLKKAG
                     VVDSGAMGFLVLIKGVATGLEYTGTRIRPIMNIATILSTSRYIRSIIKERKYESGEER
                     KKLRPLNISLNNINNISFANILTKFNNLLQRNNNHQLAEEILAQSNELDETWNPDIQY
                     RYCTEFILESDEIGKDSLKKKLESFGDSILIISSSNTYKCHIHTNKPKDLLKSLENLG
                     NISATKIDDMKVQHKNLISEDKEFYERDLAVLLIVNGEGFAKILKSLGATDILMYKQA
                     KPSVNQIGNALFKTRAKNIIVAADDSDILASLKSAITLCKSNIELIETQSVIQIISMM
                     YTFSPEMDIFSNASIMRENLDNIRFCQIAKATRQFKTDFDTIPKGNYFCLHNKSILAH
                     NSNLIDLIKLSIEKVRENESLITLYRGKMEKNNGSLIAELQQKFTQCEFEIYYGGQNR
                     YNYYITFE"
     assembly_gap    3641..3740
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4476..5750)
                     /locus_tag="PLI55_02605"
     CDS             complement(4476..5750)
                     /locus_tag="PLI55_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99759.1"
                     /translation="MKRAIFWLVILLIWFGNTAVAQITLRGFILNQDMFQTNESMDVM
                     LIRNRLRLNTETFGENVYGFASLDITNDIADSTTTLPNLREVYIDIYSNWLDLRIGKQ
                     QVVWGKTDGYFINDIVNPLDLSYFLLQDFDDIRMAMTMLNAKLHYSNHSLEVLVIPEF
                     KPMNLLFEGVWGYKRPQMMEYDSPLGKIVLPLHYQNEQIPDLSLKNAEYGLKLNTWFL
                     RTDLAFLYLRSREDKPVYFTELVNDSSGLPIRVDLTPTHPWQNFFGLNFSKPIGAFVF
                     RGEGGYYPERHFNTQDMNYLSKGMIIERPFLQFMLGGDYQLTSALDLSVQGINERILD
                     YKEGIQADENVTIGTLMLRGHFVNETVSPLILVMYNFEDYSSLTRLSIDWNYADSFTI
                     TAGVDLLAGKSDTIFGMFAKNDNVYLKFKYAF"
     gene            complement(5747..6544)
                     /locus_tag="PLI55_02610"
     CDS             complement(5747..6544)
                     /locus_tag="PLI55_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009216858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPA99760.1"
                     /translation="MKKLFSLSIICIALVIFYSSAIAQITGRDIMLKVEKQQDTKTRI
                     VKSKMILINKQNKTRERDVVQYVKSVNDPKGVDDKILIFFEYPADIRGTGLLMISYSD
                     VNKDDDRWLYLPALKKVRRIAGESKNEYFMGTDFTYDDMGGRSVDQDKHTLLGEETID
                     GVKCYKIESVPVDKNDIYSKKIGWVIPDKWVHLKVDFYDRKGQLMKTLKADDIRNVSG
                     VWLQFNLEMTNLAENHKTILKIIEAKINEPIDDQIFTITALERGGIR"
     gene            complement(6545..6901)
                     /locus_tag="PLI55_02615"
     CDS             complement(6545..6901)
                     /locus_tag="PLI55_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99761.1"
                     /translation="MTIEQKDKNIARGLTIISGGIVVYWLYQLILWLIRWFSTVRIET
                     SGIWSNFGGFCLENLSIILVVYVFICLQIAGVVEFRYRKNFTEAFFLGIIFTPIIMLI
                     YMKLKKGKFPDDSKEV"
     gene            complement(6898..9183)
                     /locus_tag="PLI55_02620"
     CDS             complement(6898..9183)
                     /locus_tag="PLI55_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPA99762.1"
                     /translation="MIHKIIEFDLKYPRTIIAFCILLTLLMGWNIPKIQLEPDIKALM
                     PQDFEIIENMKEMEETFGGNDLVVVSITADNIFSPATLEKIEAITSEIEMLENVDQVI
                     SITNVPDIVGTADGFEVRELIEEFPTTESEIDSLKKRIIGNRMIYGTLASSDFQKAGI
                     IAYLKVATDENSDEQVYQIFNAIKNKYAGPEQIHIAGLPLTRREVSATMAADVKKLFP
                     YGIILMIFLLVLTFHSWTGAFLPFTVVIMSIVFTVGLMVLFNIKMTIVEMMIPVMLIA
                     IANSYSIQVITQYFVEHLKNPDAEKDSLIRLILSYLTTPVLLSGFTTLVGFICLLSHV
                     LPPAKNLGILCACGTTIAFIYSLTFIPAALKLLGFPVTLKTASSRKRTSRFLPSWGQF
                     FTRYHKPFLGLCLVAIIIVATGIGRIIVDTNPIAYWKKSSEIRQSNEVIDRNFGGSST
                     ISVLATGDIKDPQFLKKIENLCTFLESQPTVTCANSIVDMLKRMNQAFHGDSAEYYVI
                     PETREEVAQYLFLYSLTGNTEDLDRFVDYDYTQAQILARVNDSSSKASYKLYKDTQNY
                     IAENLGTENFPKVSGMAPLIGVLSELVVDGQIRSLALSIIAVFLIIAVAFRSVKAGLI
                     AIVPLIVAVLCLFGIMGHIGITLNIATALISSIMIGIGIDYTIHFLYRFRLEARNGAD
                     AEEATIRTLTTSGKGIVYNAISVIIGFMVLMLSGFEPISFFGFLIVFSIAVCLFGALT
                     ILPALLVLTKPAFIFGKRSLQ"
     gene            complement(9180..9809)
                     /locus_tag="PLI55_02625"
     CDS             complement(9180..9809)
                     /locus_tag="PLI55_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPA99763.1"
                     /translation="MKQNSFPSGEEAKTKLSPAITYARRFILANGTRGLNIDQMAADL
                     HIAKKTIYKVFPTKEQFIEYVLLDIYHELFTETRAIQLNDNEPLKCFYGIMKIMFKHI
                     SQINPQTILDVKLFYPKVWQEVEEFEHEVINRILDSFQKADNLGLLRKKFDLKFTADL
                     IVRIVQNTFQPEIFISAPYSITEMIKIFVDIIMNGLMEKQFTFNFSDDE"
     gene            10049..10438
                     /locus_tag="PLI55_02630"
     CDS             10049..10438
                     /locus_tag="PLI55_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HPA99764.1"
                     /translation="MEKIKYGYGPHLMLDLNDCDSEVLDNLDACFKLLNELPPKIGMT
                     KITQPYVFRYEGRFPGDEGITGVVIIAESHISLHTYPKKKFVFVDLFSCKPFDVETAR
                     TYIIEYFKCQSPKWYLKERGENFPQHP"
     gene            10452..11318
                     /gene="speB"
                     /locus_tag="PLI55_02635"
     CDS             10452..11318
                     /gene="speB"
                     /locus_tag="PLI55_02635"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPA99765.1"
                     /translation="MPTPFSPGAFGALEPDYCRYEQSRIAILPIPYDGTATWQKGAAK
                     GPEALLAASANMELYDIETDSEIYRQGITTLPFLKCPKKPEDLVQKVNEKTRQLLADG
                     KFVVGIGGEHSISIGLVQAHAKHFSNLSVLQLDAHSDLRNEYERSKYNHACVMARIQE
                     ICPIMQVGIRSMDISELARLDKTRVVFAHEYRNSPEIFAKITANLTDNVYLTIDLDVF
                     DPAVFPSTGTPEPGGLGWYDVINVIAHIIQKKHIVGMDVNELLPNPSDKAPDFLAAKL
                     IYRTLSMICTKE"
     gene            11324..12343
                     /locus_tag="PLI55_02640"
     CDS             11324..12343
                     /locus_tag="PLI55_02640"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011316461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HPA99766.1"
                     /translation="MKKNDYLQKVVEHIDIKSFDARPILKAYTKMAFQARNLAVAADI
                     YDRMLKDKKCKVILTLAGSLFSAGLKNVVADMVENNMVDAIVSTGALIVDQDFFEALG
                     YKHYIGTPFSDDSELRELAIDRIYDTYIDEDELRVCDLTIAEIANSLPPRPFSSREFI
                     EEMGKYLDKVGIKTEKSVVYSAYKKGVPIFVPAFSDCSAGFGLIHHQWNRKGDMVTID
                     SARDFLELTKIKIAAKETGILMIGGGVPKNFTQDVVVATDVLGIEKPMHKYAVQLTVA
                     DERDGGLSGSTLKEASSWGKVDTKYEQMVFGEATVMMPLLVSYAYHQGNWKKRQEARF
                     SNLFV"
     gene            complement(12454..13137)
                     /locus_tag="PLI55_02645"
     CDS             complement(12454..13137)
                     /locus_tag="PLI55_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPA99767.1"
                     /translation="MENQKKAKRIINTWTGPWEKRNLPKMAAALPNWVSSDHLTILGI
                     VAALVISLGYVLTRYSNWWLMLANFGFVLHWWADSLDGTLARVRHKEREQYGYFVDHM
                     CDAFSTTIILVAFGLSPLMRLDISLFLTIGYLLLNIYAHVGAYTEGIFRLSYAKIGPT
                     EIRILVVIVNTILIFWNPTVLVLNNQPWKVVDVVGAILAGVFVIVFIIVGIKDAIKLD
                     RLDRAKWEK"
     gene            complement(13152..13568)
                     /locus_tag="PLI55_02650"
     CDS             complement(13152..13568)
                     /locus_tag="PLI55_02650"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HPA99768.1"
                     /translation="MNFKKIIKFQLVAWLGTIVNLGSLWILHGILKIPVTIAGACAIE
                     LAILHNFTWYYFRTWNGRVAHTPTDFFSRLWKYNVITAGVDFVFNLGTLSVLTHFFHL
                     HYILADILGMSIGPFIKIFINELVIFKKPKLHVKNN"
     gene            complement(13933..14005)
                     /locus_tag="PLI55_02655"
     tRNA            complement(13933..14005)
                     /locus_tag="PLI55_02655"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13970..13972),aa:Met,seq:cat)
     gene            complement(14141..15592)
                     /locus_tag="PLI55_02660"
     CDS             complement(14141..15592)
                     /locus_tag="PLI55_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002680394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPA99769.1"
                     /translation="MKQLKLDLSNLDNVFPENFTPEQIAQAKTLFFQKLSEGAHQFYG
                     GKIQIAPRAGFFGFNWFNVWYTPGVSRISTAIRDDNDLSFELTDRGNLVAIVSDSTRV
                     LGDGDVTPPGGLGVMEGKAFLMKYLGGVDAVPLCINSRDQNGLPDPDNIIQFVKMCEP
                     SFGAINLEDISQPNCYKVLDVLSEECEIPVWHDDAQGTACVTLAGLINALKLAKKEIG
                     EVKITMLGAGASNTTIARIIIAAGGDPQKIMLFDSRGGLHRNRTDIKDDSRFYRKWEL
                     CQLTNPKCVNDFAESCKGADVLIALSKPGPNVIKPEWIKSMADKAIVFACANPIPEIW
                     PHAAKEAGAFIVATGRGDFPNQVNNSLGFPGILKGALIVRARKITDSMAIAAAQALAD
                     FAEKRGITPDNIIPKMDETAVFPSEVAAVAKQAVQEGIARRIITAESAFQQAEVDIQT
                     SRAMFQTLIKESFIKELPESLLVSALEWAISQV"
     gene            complement(15614..16171)
                     /locus_tag="PLI55_02665"
     CDS             complement(15614..16171)
                     /locus_tag="PLI55_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HPA99770.1"
                     /translation="MSDICHLSTPLQNTDLIKLNIGDKVLISGKIYTARDVAHQRLVK
                     LMEIGQPLPFDIKGTVIYYAGPAPARPGNPIGSVGPTSSYRMDKFTPPLLSAGVKATI
                     GKGERSQNVIDAIIRYQAVYFVAVGGAAVVAAQAVKTARIVAFEELGPEAIYELTVVN
                     FPVFVAIDVNGNDLFRVGVARYRQE"
     gene            complement(16179..17027)
                     /locus_tag="PLI55_02670"
     CDS             complement(16179..17027)
                     /locus_tag="PLI55_02670"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008703031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPA99771.1"
                     /translation="MKNIDVAQIIPVVKKLCIDANYYVSADVLAKAQASLQSEKSPIG
                     REIFQILIQNYELAAQWQMPLCQDTGVAVVFVELGQDVHLIGGDFEQAINEGIRQGYS
                     EGYLRKAMVSDPVVKRENTGDNTPAIIYTEIVPGSSLKITVAPKGGGSENMSEVRMMN
                     ASDGLESVKDFIVDCVRRAGGRPCPPVVVGVGLGGNFEQSALLAKKALLRPLSEKNPE
                     PFWADVEQELVERINRLGIGPQGLGGSTTTWAVSIIYKPCHIASMPVAVNLDCHCHRH
                     ETAIIE"
     gene            complement(17178..17531)
                     /locus_tag="PLI55_02675"
     CDS             complement(17178..17531)
                     /locus_tag="PLI55_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPA99772.1"
                     /translation="MSMQLSVVLRTAVMLVFSNLFMTFAWYGHLRNLSDKAWYVAALV
                     SWGIALFEYLIQVPANRLGFTQMSLAQLKILQEAVTLTVFIPFAVFYMKQPLKTDYFY
                     AGLCILGAVYFIFRS"
     gene            complement(17562..18089)
                     /locus_tag="PLI55_02680"
     CDS             complement(17562..18089)
                     /locus_tag="PLI55_02680"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPA99773.1"
                     /translation="MLTIPFEKLIVKTHALWDEQNLVLASGDFKAGHFNFMTVGWGSL
                     GTMWSLPFAQVVVRPTRYTYEFMEKYETFTLCAFAAEYHKKLQIIGSTSGRRTDKVKA
                     SGITPCPARIVAAPVFQEAELQIECRKIYWSDMEPSHFLKDDIAKNYNGHDYHRIYFG
                     EIVAITGTEKYLARD"
     gene            complement(18207..19820)
                     /locus_tag="PLI55_02685"
     CDS             complement(18207..19820)
                     /locus_tag="PLI55_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HPA99774.1"
                     /translation="MAGRKYAQRNSPATALKSSFFTILIFLISLNSCSDLYQNQIPDP
                     ILPDYQGIRNMYWKIWQIMEQNIKRGNRENNFSYHYVNCESELLINQSSTIMLALFGV
                     YGSSHFPVMQSLDNFYRGQRSDGFISRIYNSMTGEYLHAPSAIEPMINPPLFTWPELK
                     YYYLTGDQSRLRKYFKIWEKYFEWLDNFCQAKGEAIGLYYNSQNGSLMINSPRTDNDF
                     GGWADMSCQMALFAEQLAETAEIIGEDTRVIYYQNRYQKITEVIREKLWHDEDGFYYD
                     LTSDGHPIRIKTTAGFWPLIAGIPSSEDAPELINHLKNDDEFARRHLFPSLSADEDGF
                     KPNGAYWRGGVWGNENYMIIAGLKRYGEYEFATTTAWNHITNMEKIFTGYFPDTSLID
                     EKYWANCNATVWELYSPENDQPGTRWDTYNLCRPNYIPTSGFGPVAMLIEDVLGFNVN
                     GPDDQLTWRLWLLNKHGIRNLKFGNNLVTLWCEKRETKESPVDIKGLTSSPFSLTIIL
                     EQDTVTIKQPRGRINISFKMEDIKKINKD"
     gene            complement(19772..21103)
                     /locus_tag="PLI55_02690"
     CDS             complement(19772..21103)
                     /locus_tag="PLI55_02690"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99775.1"
                     /translation="MEFARKFGIFSAVRKYVVSLLLLLFAGLAFSASDDFTKLPDGLH
                     YRQIKKNIRPETRPAFKAAEKYYEKKKYFQADQSYIETIQRGEGFYGNWGRACVARQE
                     KRVSDAEKFYATAFTLNDSLEGFLYDYAIFLQYNSQDWNKIEKVCTKLYTVTKDDEAL
                     SILIETAARLKQQSAALKSLEKIARRYPLRANAQVYYASILYDCGQKAEAVRVARDAV
                     QITYDPFQLKLLVTILAQEGYFVESAKVCEKMSRIAPRSAQTLEAWGFLEFQQGHYEN
                     AATYYKKALNRDYRIPTLLSLARIYTFYLNEPSKAMYYAKAITKLERNNCDALYIMAE
                     AKRRAGDIHGALKYSARLIELQPEHPQTYFYHGKLLFQNKDYKNAVKYLEKAVQYNPD
                     IKRYRLVLAKAYAGAGMSEKARATYANYLNEPLKNLWQEENMLRETPPPPR"
     assembly_gap    21490..21589
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21590..22054
                     /locus_tag="PLI55_02695"
     CDS             <21590..22054
                     /locus_tag="PLI55_02695"
                     /inference="COORDINATES: protein motif:HMM:NF021546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HPA99776.1"
                     /translation="GNKLGASFLGFFEISIWLLAISQVMQQGLNNVASFFAYGLGFAC
                     GNFIGITIEEKLALGLQTIRLISTETVDILTMTLRDAGYGVTVINASGAKGPVHILYV
                     IVQRKKVKDVIRIASEITPDIFISVEDIRSVKAGFFPETNRNFKWWFWNKNK"
     gene            22096..22617
                     /locus_tag="PLI55_02700"
     CDS             22096..22617
                     /locus_tag="PLI55_02700"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPA99777.1"
                     /translation="MKFYRKSLFIAYLIGFILLGIDFLTKSIANLRLPFQERVDTALP
                     FLSWFLTYNTGYHFIFGNISNQKIWAIFGIALVAVLGFSLSRSLIKEKESKANVIILT
                     IILSLIIGSAGNVLEVLIKGHATDFFLFHPFPWPSNLCDQYINAIIYIMLPITIIKSI
                     LDWRQSKKKMAQN"
     gene            complement(22720..23634)
                     /gene="argF"
                     /locus_tag="PLI55_02705"
     CDS             complement(22720..23634)
                     /gene="argF"
                     /locus_tag="PLI55_02705"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPA99778.1"
                     /translation="MKRDFLHITDFSTAEIWEVFELTKKLKSYLKEGWEYTPFKGKTM
                     AMIFAKPSARTRISFETGMTQLGGHAIYLGPNDIGLGQREAVKDIAQVVARYDDIIMA
                     RLFAHAHILELAEFSSVPVINGLTDYNHPCQVMADIFTVLEKRGNLDNLKVTFVGDGN
                     NVVNSWLELTLRLPMHFVLACPPGYEPDKELLTKAQNSGLSKVEISHEPFEAVQNADV
                     IYTDVWASMGRETEKEKRKCDFAGFQVNLQLVNAAKPNVLIMHCLPAHRGEEISDEVI
                     DGPNSIVFDEAENRMHVQKAIIHKLMVG"
     gene            complement(23660..25024)
                     /gene="hslU"
                     /locus_tag="PLI55_02710"
     CDS             complement(23660..25024)
                     /gene="hslU"
                     /locus_tag="PLI55_02710"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPA99779.1"
                     /translation="MKNRELTPREIVRELDAYIIGQEKAKKAVAIAMRNRWRRQHVRG
                     AIKEDILPNNIILIGPTGIGKTEIARRLSNLAGAPFIKVEASKFTEVGYVGRDVESII
                     RDLTGIAVTMVKNERMAEVNSQALFLANERILDILLPSGETKHHSEQEEIIPRGDEKF
                     NRTREKLRVMLENGEMEDRLIEITVNQGAMPMMQIFGPAGIEEMEMNLSEMLGSAFPQ
                     KKKIQKVTISEARKIFTQEEAQKLIDMETVVREAVQRVEETGIVFIDEIDKIASSGDQ
                     RGYGPDVSREGVQRDILPIVEGSNVQTKYGVVRTDHILFIAAGAFHATKPSDLIPELQ
                     GRFPIRVEMDSLGKDEFVKILKNPRNALLKQYTALLKTEKVDLIFDDDAVEEIAQIAA
                     EVNEKMENIGARRLHTILTTLLEEILYKVPDKDIREVHITREFVQARLQNIVRDRDLS
                     RYIL"
     gene            complement(25021..25569)
                     /gene="hslV"
                     /locus_tag="PLI55_02715"
     CDS             complement(25021..25569)
                     /gene="hslV"
                     /locus_tag="PLI55_02715"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HPA99780.1"
                     /translation="MQEKPIPRIIATTVIGVRRNGQAAMGSDGQVTIGETIMKHTTKK
                     VRRIFNDQVVVGFAGASADAMALFDKFENELEKYKGNVNRAAVELAKIWRTDKYLRQL
                     EALLTVMNKETMLIISGTGDVIEPDDDIIAIGSGGAFATAAARALLRHTEMNAREIVA
                     ESLRIASEICIYTNNNFTIIEL"
     gene            complement(25582..26202)
                     /locus_tag="PLI55_02720"
     CDS             complement(25582..26202)
                     /locus_tag="PLI55_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPA99781.1"
                     /translation="MRFKSFIWLLILALIIIGAERATVILHEDEQMIVARFTQPTVTP
                     VTTAGVHLIIPFLAKTYIYPNNLIEWQSNVIEMPTKDDKFIAIESFAYWKISDPLLFY
                     ERVRTIPEAENRLTDLLDGSLRDEVARYTLAELIRSSSRRMDITEIKDILKVPQSRTL
                     FNVKGRRPAIIAGILKNATATLDSLQLGIQLVDFDLKSVNFVRIIE"
     gene            complement(26267..26989)
                     /locus_tag="PLI55_02725"
     CDS             complement(26267..26989)
                     /locus_tag="PLI55_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HPA99782.1"
                     /translation="MAGKKVQFSNKEIEVSSVSYWLIILIALVLVVIVTALMSFYTIE
                     PGETGVVTRCGKVHQINGPGAHWKLPWGIDRLYRVKVNYPYTAEFGIRTTSDSLTALS
                     DNFYLPEARLLTGDLKMVDLQWVVQYSIQDPVAYLFGVENPENMLRLVGAAAMRSAVG
                     DFSFREVFQTERRAVAERAKNYMQTKIDLYKTGIVIQHVQLKEARPALPLSEPDTTIS
                     LKPFNQTDSTEADSVKKIGENW"
     gene            complement(27021..28412)
                     /gene="rpoN"
                     /locus_tag="PLI55_02730"
     CDS             complement(27021..28412)
                     /gene="rpoN"
                     /locus_tag="PLI55_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPA99783.1"
                     /translation="MMQLSQSQVQKLTQELRLTPQQILQSTILQLNTLALEARVLQEL
                     EQNPVLEEVNPEVSESSEEELQDNQETSQEDDIDWEEILPQNDDYTPKEERDSDQKKT
                     QFVQPAPRGLVEHIFDQLKLHNLDDEEERIAAEIVWNLDENGYLTLPIENIAFGLGLP
                     QKKVLKVLKEIQELDPPGLAARDLKECLLIQLNQIPNSDDAIKVISEHFEDFANHRYQ
                     NIMKAYGWDKERMQAVQEMITKLNPKPGATLNTLDPQYIVPDLIVEKVDGEYIVEVND
                     TRIPELQISSTYLQMISNGSKIDNETKNYLKRKIDNAKWFIQAIQQRRLTMIRVMKAI
                     IARQLDFFEDPSKPLKPMILKDIADDVNMDISTISRVTNNKYVQLPSGIYELKYFFNE
                     GIKDEDGEEISTRIVKEKLKEIIDNEDKHKPYSDEQLSNLLKQNGYLVARRTVAKYRE
                     QMKIPVARLRRVV"
     gene            complement(28409..29161)
                     /gene="lptB"
                     /locus_tag="PLI55_02735"
     CDS             complement(28409..29161)
                     /gene="lptB"
                     /locus_tag="PLI55_02735"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPA99784.1"
                     /translation="MELNNQPQKKTLRGENLVKIYGKRRVVDSVNIEVSKGEVVGLLG
                     PNGAGKTTTFHMFTGMIKPTSGKVFINEQEITRLPMYKRAQLGIGYLSQEPSVFRKLT
                     VEQNLMLVLQTLGISRKEQKERLEILLDQLELRKLRKRRADTLSGGERRRTEITRALI
                     TNPDFILLDEPFVGVDPIAVEDIQKIVVSLKDRDIGVLITDHNVHETLSITNRSYLMF
                     EGRILKSGTSEFLANDEDARRLYLGEAFKLAR"
     gene            complement(29165..29704)
                     /gene="lptC"
                     /locus_tag="PLI55_02740"
     CDS             complement(29165..29704)
                     /gene="lptC"
                     /locus_tag="PLI55_02740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPA99785.1"
                     /translation="MKGRFLISTLFVVLFCWGCTEDKSYEPTMIDRQYPDQEIWNSNI
                     TLTKDGQKRAVVHSGHLTKNNDQALIYMDTDVAVDFYDSEGHHLSHLTSQTATVNEHT
                     NDLIAKGNVVVVSDSGFTLFTEELRWDHRRERIISEVFVTFVTPEDSLTGIGFESESD
                     LKNWVIFKPSGVTSRELKY"
     gene            complement(29701..>30360)
                     /locus_tag="PLI55_02745"
     CDS             complement(29701..>30360)
                     /locus_tag="PLI55_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPA99786.1"
                     /translation="QLIPPLKKMRIPIIGLLGRNESTLAKKCDYVISTQINGEADPLQ
                     LVPTTSAIATLAMGDALAISLLAKRHFNRRDFAGLHPSGSLGRRLLTTVADLMHTGEE
                     IPFVKSSDKMPEVLYMMTKKSLGIVGVLDDSENLIGVVTDGDLRRGLERQKDFMEQTA
                     IKVMSHNPKWIKKSSLAIEALHLMEEHSITNLFVYNGEQNRKPIGIIHIHDILRYGIM
                     P"
BASE COUNT         8665 a         6678 c         5865 g         8952 t
ORIGIN      
        1 ggttttttaa aattattata aaggagagta aaatgaaaaa attgctggta attttgatgg
       61 ttgccagttt ggcgcttgtc acggtgagtt gccagaaaaa gaccaaagta gaagaggcac
      121 cggctccggt cgacacgact actgcggtta ctgatactgt taatgcgtaa tcatcgttaa
      181 cagaaatcac cgattaagtt tacacaaaat atcccgctcc ggcggggtat tttgttttta
      241 tagctaattg taagctgtat aaaattaagc caatactaga tattttattc attttgccaa
      301 tgtggtaaaa gtacatccct attagactat tttttggtag ataacaattc ttctaaacca
      361 ctcccggttc ataaaatcct ttttattaca cgtagcgaag caatctttat tgctatagaa
      421 atttaatttt ctggaattag tttctcatct ctttttgaga tattgatgat aaggttgaga
      481 accccggtta agagtacgag atatacgact ccccaaatga ttgtagctgg ttttttgaaa
      541 atagcagcta caataggaga aaagccgagg atgaacacag gagtgaagcc cattctacgg
      601 atacaataag caatgagagc cgcgggaata cagctgataa gtacttcgag cggaataaag
      661 cataaaagaa tcccaagagc ggcagaagcc cctctgccac cataaaattt gtagtagatc
      721 ggaaaacaat gcccaataac agcaaaagaa gccgcaaata tggttggaaa agtatcaagt
      781 ccaatgagtt tgccgatgac aactactacg actgctttta gcgcatccgc tattcccgcg
      841 agtaagccta ttcctttgcc cacattctca aatacattta acgctccgga atttttgacc
      901 ccaaccgttc taatgtcttt tccggtgcgt accttggtaa aaatatagct aaacgagata
      961 gatcccaaaa aataagcaac tatggcaaga attactgttt ggtaaatcat tattctaatt
     1021 tcttatttta agacaaaact cccaccccac gtacctgggc ctccatgaac agcctcaaca
     1081 aatctggcat gtccatgaac gagtcttcga taagtgaggt tatcatttat ttgcttttct
     1141 aagtccttga gctcatcggc atcactgttt tcaaaatgca gtatatttat aagctcaatt
     1201 ttagccacct tctttagaac cattttctgt ttaatatgat tgataattat cttattagcg
     1261 gcttggtatg ttcttccttt tcctacaggt aaaaatcctg aatcagtttc atcgccaaat
     1321 aaccccacta ctggtattat cctcaatact gagccgagca gagactgtgc ccttccaatt
     1381 cttccccctc tataaagata attcaagtcc ggcacacaaa aatacgagta ggtatttttt
     1441 attacttcgt ctatgcgcga gacaaattca tctacactgt ccgactcttt taggagttct
     1501 ataacccgca gaagtaccgc cccaactccg gaagaggctt gtttcgtatc aatattaata
     1561 attgggataa tgccttcata gtctttgcgg attccttctg ccatctggac tgttgccgaa
     1621 gacatattac ttcccattaa cacatgaaca atcagatcat atttatcctt gttcttttca
     1681 attacctcaa cgatgtcttt tctcagcaat gcctgagagc gagccacgat tttctctttc
     1741 ttgaaacgct caatgagata ttgagcagtg tgtgtttcgt cttcaaaata ttctttttca
     1801 tcaattacca caggaaattt tactaccgca acgtccgggt aatcaatttg ctcatttttt
     1861 aatccgagat tatcgcctgt cagtataagt attttctgat ccatttcgat tctccttatt
     1921 caaaggtaat ataatagttg tagcgatttt ggccaccata atatatttca aactcgcatt
     1981 gcgtgaattt ttgttgcaac tctgcaatca gcgatccgtt gtttttttcc attttgcctc
     2041 tgtataaggt aataagcgat tcattttccc tcactttttc tatggaaagt ttgataagat
     2101 caatcaagtt tgaattatgg gcaaggatgc ttttattatg taggcagaaa tagttgcctt
     2161 taggtattgt atcaaaatcg gttttaaact gccgcgttgc ttttgctatt tggcaaaacc
     2221 taatattatc gaggttttcc cgcatgatac tggcatttga aaaaatatcc atttcaggtg
     2281 aaaatgtgta catcatactg attatctgaa tcacactctg ggtttctata agttctatat
     2341 tacttttgca tagcgtaatg gcagatttga gactggcgag gatatctgag tcatcagccg
     2401 caacaatgat gttttttgcc ctggttttga ataaggcatt ccctatttgg ttaacggaag
     2461 gttttgcttg cttatacata agaatatcgg ttgctccgag gcttttcaag attttagcaa
     2521 atccctctcc attgacaata agtagcaccg ccagatcgcg ttcatagaat tctttatctt
     2581 ccgatataag gtttttatgc tgaaccttca tatcgtctat tttagtcgct gagatattcc
     2641 caaggttttc taaagattta agaaggtctt taggtttgtt agtatgaata tggcacttat
     2701 aagtattgct agaagaaata atgaggatgc tatctccgaa tgattccagt ttctttttta
     2761 gtgaatcttt tcctatttca tctgattcga ggatgaattc agtgcaatac cgatattgta
     2821 tatccgggtt ccaggtttca tccagttcat tactttgcgc taaaatctct tcggccagtt
     2881 gatggttatt attgcgctgg agcaaattat taaacttggt cagaatattt gcgaaggaaa
     2941 tattgttaat attatttagg gaaatattca atggtcgtaa ttttttccgt tcttctccag
     3001 actcatattt tcgctctttt ataattgaac gaatataacg gcttgtcgag agaatggtgg
     3061 caatattcat aatcggacga atccttgtac ccgtatattc cagtcccgta gccactcctt
     3121 tgataagcac cagaaatccc atagcaccgg aatccaccac acctgctttt ttaagaacgg
     3181 ggagcatttc aggagtcttc tccaacgttt cttccagatc ggggacaata tctttgataa
     3241 tagaaagggc atccttgcca tccattacca gttcagaaaa ccgctctgca aatactctca
     3301 tcatagtgag catcgtgcct tctttcgggt tggcagtctc ggcataggcg tatttatatc
     3361 ccgaaataag tgcttgcgca atctccgtgt taggtatttt ctgatctgga ggccagtctg
     3421 ctagtttttt cgccacccct tggataaata gagccagaat aacacccgaa cacccgcgac
     3481 tatttagtgt cattccttcg gcaagggcgg ttagatattc cgataaagtt gattggtcgg
     3541 aggcataatt tttggcatga gccaagccac cctgcaccgt aagaaccata ttaagacccg
     3601 tatcaccgtc gggaacggga aaaacattca ggtcgttaat nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn ccatattaag acccgtatca ccgtcgggaa cgggaaaaac
     3781 attcaggtcg ttaattcggt tcttttcaat agagagcact gtcagtgcgt cctttagaat
     3841 gctattgaga actgaagctg ttatttccat ttccactctc tcaaaaattt cccacacttc
     3901 aacatttagg aattcaaata agcaatattg tttattttac ataatagctt cgattgttgc
     3961 aaattcattt ttctatattg gtgttaattt tcaggatgtg gtattcactt tttagaaatc
     4021 cagattaatg gaaaatagct tttggtaagg cggaaagatg tttgagtgta accaaacacg
     4081 ccaaagttaa ttaaaagata ttgactataa ttctaaaaat caaaatgaac aatggattcg
     4141 gtctgtcctt cattgtcaac tgaaattgcg atcaatcaaa ataaaaacta aatgttgccc
     4201 ttgttccagt gcgattacca ttcaccgatt tatagctgtt agcttttgat aaatcgaggt
     4261 aagtaacgga cggaccggct gagtaaaata cttccgtgac agccctaact aacattcatt
     4321 aagacgagta aatttcttaa aaagattgag gttcgttgaa atcttttcaa ctatctaaaa
     4381 cttcttccca ctatattctg ggaaaaaata ctcctcctat tcctataagt gatatttttt
     4441 aatttataaa tattggctga aaagtatgct tttaactaaa aagcatactt aaacttcaga
     4501 tagacattat cgtttttagc aaacatccca aaaatcgtgt cagattttcc cgctagtaaa
     4561 tctactcccg ccgtgatcgt aaagctatcc gcataattcc aatcaatact caagcgcgtc
     4621 aatgaagagt aatcctcgaa attatacatc actaaaatta gcggtgaaac agtttcatta
     4681 acaaaatgac ctctcagcat caacgtacca atagtgacat tttcatcggc ttgaataccc
     4741 tctttataat ccagaattcg ttcgttaatt ccttgaacag ataaatccaa agccgatgtt
     4801 aactgataat cgccaccaag cataaattgt aaaaaaggcc tctcgattat cattcctttt
     4861 gatagatagt tcatatcttg agtgttgaaa tggcgctcgg gataatagcc gccttctcca
     4921 cggaacacaa atgccccgat tggcttggag aaattcaatc cgaagaaatt ctgccaggga
     4981 tgcgtcggtg tcaaatctac tcgaattggt aaacccgaag agtcattaac aagttcggta
     5041 aaatacacag gcttatcttc acgactacgc agatataaaa atgccaggtc ggttctcaaa
     5101 aaccaggtat ttaatttcaa accatattca gcatttttta acgacaggtc aggaatttgt
     5161 tcattctgat aatgcaaagg aagtacaatt ttgcccagcg gactgtcgta ctccatcatt
     5221 tgtggccttt tataacccca aacaccctca aacagcagat tcatcggctt gaattccggg
     5281 attacgagca cttccaacga gtgattgctg tagtgtaatt tggcattcag catagtcatt
     5341 gccatgcgaa tatcgtcaaa atcctgtagg agaaaatagg ataaatccag cggattgacg
     5401 atgtcgttga taaaatagcc gtcggtttta ccccagacca cctgctgttt gccgattcta
     5461 aggtcgagcc aattgctata aatgtcaatg tacacttccc gtaaattcgg caacgtcgtc
     5521 gtgctgtcgg ctatgtcgtt ggtaatatca agcgacgcaa aaccataaac attttcacca
     5581 aaagtttcgg tgttcaatct cagacgattg cgaatcaaca taacatccat tgattcatta
     5641 gtttgaaaca tatcctgatt gagaataaaa cctcgcagag taatttgcgc tacggctgta
     5701 ttgccaaacc agattaacaa aattaccagc cagaagattg cccgtttcat cggatgcctc
     5761 cgcgttcgag ggctgtaatc gtaaatattt ggtcatcaat aggttcgttg attttagcct
     5821 cgataatttt gagaatggtt ttgtgatttt cagccaaatt ggtcatttca aggttaaact
     5881 gcaaccagac gccggagaca tttcgaatat catcggcttt gagggttttc atcagttgac
     5941 ctttccggtc atagaaatct acctttaggt gcacccattt gtccggaatc acccagccaa
     6001 tttttttact gtaaatgtca tttttgtcta ccggaaccga ctcgattttg taacacttca
     6061 cgccatcaat cgtctcttcg ccgagtagag tgtgtttatc ctgatcaacg cttcgcccac
     6121 ccatatcatc ataagtaaaa tccgtaccca tgaaatattc attcttgctt tcaccagcga
     6181 tgcgccggac tttttttaat gcgggcaggt aaagccagcg gtcatcatct ttattgacat
     6241 ccgagtagga aatcatcagt aatcccgtgc cacgaatgtc ggctgggtat tcgaaaaata
     6301 ttagaatttt atcatcaacg cctttgggat cattgaccga tttaacgtat tggacgacat
     6361 cccgctcacg ggtcttattt tgcttattga tcaagatcat tttcgactta acaatccgcg
     6421 tcttagtatc ctgctgtttc tcgactttca gcataatgtc gcgcccggtt atttgggcta
     6481 tagcggaact ataaaaaatc acaagggcga tgcaaattat acttagagaa aaaagctttt
     6541 tcatctaaac ctcctttgaa tcatcaggaa atttcccttt ttttaatttc atataaatca
     6601 acataattat cggagtaaat attattccta ggaaaaaagc ctccgtaaaa tttttccgat
     6661 aacgaaattc aaccacacca gcaatttgca aacagataaa tacataaact actagaataa
     6721 tgctcagatt ttccaggcaa aacccgccga aattcgacca tatcccagac gtttcaatcc
     6781 taacagtact gaaccaccga attaaccaga gaatcagctg atatagccaa tagactacaa
     6841 tgccaccaga aattatcgtt agcccgcgcg ctatattttt atctttttgt tctattgtca
     6901 ctgtaaactc cttttaccga atataaaagc cggtttggtc aggactaata aagccggtaa
     6961 aatcgtcaag gcgccaaaaa gacaaacggc aattgaaaac acaatgagaa atccgaaaaa
     7021 gctgatcggc tcaaatccgg ataacatcag gaccataaaa ccaataataa ccgagatggc
     7081 gttataaaca attccctttc cactggttgt aagcgtgcga atagtagcct cttcagcgtc
     7141 agcgccgttg cgcgcttcca gtcgaaaacg atataaaaag tgaattgtgt aatcaatccc
     7201 aattccaatc ataatactgg aaattaaagc cgtcgcaata ttaagagtaa taccgatatg
     7261 tcccataatc ccgaacaaac acaaaaccgc cacaattagc ggcacgatag cgatcaatcc
     7321 cgcttttacg gagcgaaagg ctaccgcaat aattagaaaa acagcaataa tcgaaagtgc
     7381 caaacttcgg atttggccat caacaaccaa ctccgataag actccgataa gcggcgccat
     7441 tccagaaact ttagggaaat tttctgtccc taaattttca gcgatataat tctgcgtatc
     7501 tttatacaat ttatatgagg ctttcgaact ggagtcgttc actcgtgcca ggatttgcgc
     7561 ctgggtataa tcgtaatcca caaagcgatc cagatcctcg gtattgccag tcaatgaata
     7621 gagaaaaaga tactgcgcta cttcctctct ggtctccgga ataacataat actcggcact
     7681 gtcgccgtga aaagcctgat tcatccgctt aagcatatcc actatcgaat tggcgcaggt
     7741 gacagtcggc tgactctcca gaaaggtaca aaggttttct atcttcttta aaaattgagg
     7801 gtcttttata tcgccagtag caagtactga tatagttgaa ctgccaccga agtttcggtc
     7861 aattacttca ttgctttgac gaatttcaga agattttttc caataggcaa ttggattagt
     7921 atcaacgatt atacgtccga ttccggttgc gacgataata attgccacca agcaaagtcc
     7981 cagaaatggc ttatgataac gagtgaaaaa ttgcccccag ctcggcagaa accgactggt
     8041 tcgcttacga gaactggcgg ttttcaaggt taccggaaaa ccaagcaatt ttaacgccgc
     8101 cggaatgaaa gtcaggctat aaataaaggc aatagtcgtt ccgcaggcac ataatatccc
     8161 gagatttttt gccggtggta aaacgtgtga tagcaggcaa ataaatccaa ccagcgtcgt
     8221 aaatccggaa agcagaactg gcgtcgttaa atatgacagg atcaatcgga ttaaactgtc
     8281 tttttcagca tccggatttt tgaggtgctc gacaaaatat tgggtaataa cctgaatact
     8341 ataactatta gcaatggcga tgagcattac cggaatcatc atctcgacga ttgtcatttt
     8401 aatattgaac aaaaccatta aaccgacagt aaatacaata ctcataatta cgacggtaaa
     8461 cggtagaaat gctccagtcc aactgtgaaa agtcaacacc agcaggaaaa tcatcaaaat
     8521 aataccatac ggaaagagtt tcttgacgtc cgcggccatc gtcgccgata cctctcgacg
     8581 agtcaaaggc aatccagcaa tgtgaatctg ctccggtccg gcatatttat ttttaatagc
     8641 gttgaaaatc tgataaacct gctcgtcact attttcatca gtggcaacct tcaggtaggc
     8701 aatgattccc gccttctgaa aatcagacga agccaaagtc ccataaatca ttcgatttcc
     8761 aataatgcgc ttcttcagcg aatctatctc cgactcggtg gttggaaatt cttcaatcag
     8821 ttctcgcacc tcaaatccgt cggcagtgcc aactatatcc ggaacattag taatcgaaat
     8881 gacttgatca acgttttcca gcatttcaat ttccgaagtt atcgcctcaa ttttttccag
     8941 cgttgctgga gaaaagatat tatccgcagt aatcgaaacc acaactaaat cattgccgcc
     9001 gaaagtttct tccatctcct tcatattttc gataatctca aaatcctgcg gcataagggc
     9061 tttaatatcc ggttcgagct ggatcttggg aatattccag cccatcagta gagtcagtag
     9121 aatacagaaa gcaatgatcg ttcgcggata tttcaggtca aattcaatta ttttgtgaat
     9181 cattcatcat ccgaaaaatt aaaggtgaat tgtttctcca tcagtccgtt cataattata
     9241 tctacaaata tttttatcat ctcggtaatc gaatagggcg cactaatgaa tatctccggc
     9301 tgaaaagtgt tctgaacaat tcttacgatt aaatcggctg tgaatttgag gtcaaatttc
     9361 ttgcgcaata gtccgagatt atctgccttt tgaaaagaat ccagaattcg attgattacc
     9421 tcatgttcga attcttctac ttcctgccac actttgggat aaaacaattt tacatcgaga
     9481 attgtttgcg gattaatttg agagatatgc ttgaacatta ttttcattat tccataaaaa
     9541 cacttaagcg gctcattatc atttagctgg atagcacggg tctcggtaaa caattcatga
     9601 taaatgtcga gaagaacata ctcgatgaat tgttctttgg tagggaatac tttgtaaatg
     9661 gtctttttag cgatgtggag gtcagcagcc atttggtcaa tattcaagcc gcgggtgcca
     9721 ttggctagga tgaatcggcg agcgtaggta attgccggag agagtttagt tttggcttct
     9781 tcacccgatg gaaacgaatt ttgtttcata cgtttccaat ctaaaatatt ttaaaattaa
     9841 agtcaatagc taattttgca atgaatcaat ttaagattaa aaactttctc cgtcgtaaat
     9901 catctaattt tttacctaat aatttttatt tgaaatatta aaattttaat gtataatagc
     9961 gcgttttttt acagatttca aaacgatgtt gatcgtcgga ttgaaattcg ttgtcgtaag
    10021 taactcatta atggagggtt tatgcgaaat ggaaaaaatc aaatacggct acggacctca
    10081 cttaatgctg gatttgaatg attgcgattc agaagtatta gataatctcg atgcttgttt
    10141 caagctatta aacgaactcc cccctaaaat cggcatgacc aaaattactc aaccttacgt
    10201 tttccgttat gaagggcgtt ttcccggtga cgaaggtata acgggcgtcg tgatcatcgc
    10261 cgaaagccat atttcattac acacttaccc caagaaaaaa ttcgttttcg tcgatctgtt
    10321 ttcctgcaaa ccattcgatg ttgagacggc tcgcacctat ataatcgagt actttaaatg
    10381 ccagtcgccg aaatggtacc taaaggaacg cggcgaaaat ttcccccaac atccttagtt
    10441 taacctaaac tatgccgact cccttttcac ctggagcatt tggtgcgctc gagccggatt
    10501 actgccgata cgagcaatcc cggatagcca ttctaccaat tccttacgac ggaacagcca
    10561 cttggcaaaa aggggccgct aaaggtcctg aagcattatt ggccgcctcg gctaatatgg
    10621 aactttacga tattgagacc gattcggaga tttatcggca aggcatcact acgctaccct
    10681 tcctcaaatg ccccaaaaag ccggaggatt tagtccaaaa agtcaacgaa aaaaccaggc
    10741 agcttctcgc cgatggaaaa tttgtagtcg gtatcggcgg cgaacactcg atttcgatcg
    10801 gactggtaca ggctcacgcc aagcacttct ctaacttgtc agtcttgcag ctggatgccc
    10861 actccgattt gcggaacgaa tatgagcgta gtaaatacaa tcacgcttgc gtaatggcac
    10921 gtattcaaga aatctgccca attatgcagg tcggtattcg tagtatggat attagcgaat
    10981 tagcgcggtt agataaaaca cgagtggttt ttgcgcatga ataccgcaat tcaccggaga
    11041 ttttcgcgaa aatcactgcc aatttgaccg acaacgttta tctgaccatt gatctggatg
    11101 ttttcgatcc ggcggttttc ccctctaccg gtactcctga accgggtgga ctgggctggt
    11161 acgatgttat caacgtcatt gcgcatatta ttcaaaaaaa gcacatcgtc ggtatggacg
    11221 ttaatgagct tttgcctaac ccatctgaca aagcccctga ttttctagcg gctaaactca
    11281 tctatcgaac cttaagtatg atttgtacaa aggagtaaat gaaatgaaaa aaaacgacta
    11341 tctgcaaaag gtggttgaac atattgatat taaatcattc gacgcgcgtc cgattctgaa
    11401 agcctatact aaaatggctt ttcaggcacg caatttagct gtcgcggctg atatttatga
    11461 ccgaatgcta aaagacaaaa aatgtaaagt catcctgact ctagccgggt cattattcag
    11521 cgccggattg aaaaacgtcg tagctgatat ggtcgaaaat aatatggttg atgccatcgt
    11581 ttcaaccgga gctttgatcg ttgatcagga tttcttcgaa gcactcggtt ataaacatta
    11641 tatcggaact ccttttagtg acgatagcga attacgtgaa ttggctattg accgcattta
    11701 tgacacttat atcgatgaag atgaactgcg ggtctgcgat ctgaccatcg ccgaaatcgc
    11761 taactctctg ccaccgcggc ctttttcttc gcgcgagttt atcgaggaaa tgggaaagta
    11821 tctcgataaa gttggtatca aaaccgaaaa atcggtcgtc tattcagctt ataaaaaagg
    11881 tgttccgatc ttcgtccctg cgttttcgga ttgttcggct ggtttcgggc taattcacca
    11941 tcaatggaat cgcaaaggcg atatggtaac gattgattcc gctcgtgatt tcctcgaact
    12001 gactaaaatc aaaatcgccg ctaaagaaac cggcattcta atgattggcg gtggtgttcc
    12061 caaaaatttt actcaggacg ttgttgtagc cacagacgtg ctcggaatcg aaaaaccaat
    12121 gcataaatat gctgttcagt taaccgtggc tgacgaacgc gatggtgggc tatctggatc
    12181 aaccctcaaa gaagccagtt cctggggcaa agtcgacact aagtacgaac aaatggtttt
    12241 cggcgaggct acggtgatga tgccgctctt ggtcagttat gcttatcatc agggtaattg
    12301 gaaaaaacgc caggaagccc gcttcagtaa tctttttgtt tagtaatctt aacccaaaca
    12361 gtcgttatac tgaaattata gtaagggagt aaaaggttaa ttggtcatct ttcccctacc
    12421 aaaaataata gattaattac actagttgct tgattatttt tcccatttag cacgatcgag
    12481 gcggtctaat ttaattgcgt ctttgatgcc aacaattata aaaactataa cgaaaacccc
    12541 agccagaatc gcaccgacaa cgtcaactac tttccacggt tgattattta acaccagcac
    12601 agtaggattc cagaaaatca gtatcgtatt tacaattacc accagaatac gaatttcggt
    12661 cggaccgatc ttggcgtatg ataagcgaaa aataccttcg gtataagcgc cgacgtgggc
    12721 ataaatattc agcaacaaat aaccgatcgt gagaaacaga ctaatgtcta aacgcatcag
    12781 cggtgaaagt ccaaacgcaa ctagaataat cgtcgtcgaa aaagcgtcgc acatatgatc
    12841 aacgaaatac ccgtattgtt cccgctcttt atggcgcacc cgcgccaatg ttccatcgag
    12901 gctgtctgcc caccaatgta aaacaaagcc aaagtttgcc aacattaacc accaattcga
    12961 atatctggtt agcacatatc caagcgaaat tactaaagcc gccacgatac ctaaaattgt
    13021 aagatggtcg gaagaaaccc agttcggtag agcagccgcc atcttaggaa gattgcgttt
    13081 ttcccaggga ccagtccagg tattaatgat tcgtttggct ttcttttgat tttccattat
    13141 gtctttttcc cctaattatt tttcacatgc aatttaggct tcttaaaaat aaccagttca
    13201 ttaataaata ttttaataaa tggacctata gacatcccca aaatatcagc taaaatataa
    13261 tgaagatgaa aaaaatgggt aagcaccgat aatgtaccca aattgaaaac aaaatccact
    13321 ccggctgtaa taacattgta tttccagagt cttgagaaaa aatcagtggg agtgtgggca
    13381 actctaccat tccaggtgcg gaaataatac caggtaaaat tatgcaaaat agctaattca
    13441 attgcgcagg ctccggcaat cgtcaccggg atttttaata taccgtgcaa tatccaaagt
    13501 gaaccaagat taacaatcgt tcccagccag gctacgagtt gaaatttgat aattttcttg
    13561 aaattcattt aattagttac gttataaatc aaatagtcaa aatgctcata caggcgcaaa
    13621 tataaaatat ttattatagt tttcaaactc taatatcttt gctggaaatc cttaataaat
    13681 tcgaagtcca tattttctaa taatcaatac cttgcgcctt gcgccagcgt ttgattagac
    13741 tttgagtaac tttgtagcgg ggactggatt cgaaccagtg tcccgatccg tcgggatatg
    13801 agcccaacga gttccaacga gctactttac aggtaatttt acttaaggaa gaacattcaa
    13861 aaagattata cagttttaaa ataaaaactt tgcgctcttt gcgagagatt aaatggactt
    13921 taaattaatt tgtagcgggg gtaggattcg aacctacgac ctttgggtta tgagcccaac
    13981 gagctaccaa ctgctccacc ccgcgatcaa ttttaaaaat cgggcataat ttgcataaaa
    14041 tttggcacaa taacaataat caaaaattgt tttattcaaa caaaatcaca gaattccatt
    14101 ttgctaaaaa tcggattgct tttaatcggc tccctgccaa ttagacctgg ctaatcgccc
    14161 attcgagggc tgaaaccaac aaactctcag gaagctcttt gataaaactt tcttttatca
    14221 gggtttgaaa catcgcccga gaggtttgaa tatctacttc ggcttgttga aatgccgact
    14281 cagcagtaat tattcgccgg gcaattccct cttgaactgc ttgcttagct accgccgcga
    14341 cttcagatgg aaaaactgcg gtttcatcca ttttcggaat aatattatcc ggtgttatgc
    14401 cacgcttttc ggcaaaatca gccaaagctt gtgccgccgc aattgccatc gaatcggtta
    14461 ttttacgagc tcggactatc agagcccctt ttaaaatacc aggaaagccc agtgaattat
    14521 taacttgatt gggaaaatcg ccacgaccag tagccacaat gaaagcaccc gcttctttgg
    14581 cggcgtgcgg ccaaatttcc ggaatgggat tagcgcaagc gaaaacaatt gctttatcag
    14641 ccatagattt gatccattcc ggcttaatga cattcggtcc cggttttgag agtgcaatca
    14701 acacatccgc ccctttacat gattcggcaa aatcattgac gcattttgga ttggttaatt
    14761 ggcataattc ccatttacga taaaaccgcg aatcgtcttt aatatccgta cgattgcgat
    14821 ggagccctcc tcgggaatca aagagcataa tcttttgggg atcgcctcca gctgcaataa
    14881 taattctggc tatagttgta ttcgaagcac cagcccctaa cattgtgatt ttcacttcgc
    14941 cgatctcctt ctttgccaat ttcaaggcat taattagacc ggctaaggtc acacaagctg
    15001 tcccttgagc atcgtcatgc caaactggaa tttcacattc ctcgctgagc acatccaaaa
    15061 ccttataaca attcggctga gaaatatctt ctagattgat agctccaaaa ctcggttcac
    15121 acatcttgac aaattggatg atattatcgg gatcgggtaa gccgttctga tcgcgactat
    15181 taatacagag cggtacggca tctactccgc caagatattt cattaaaaag gctttgccct
    15241 ccataactcc cagacctccc ggtggagtca catctccatc accaagcacc cgagtcgaat
    15301 cgctgacaat cgctactaaa tttccgcgat cagtcaattc aaaagagaga tcattgtcat
    15361 ctcgaattgc agtcgaaatg cgcgagaccc ccggtgtgta ccagacatta aaccagttaa
    15421 atccaaaaaa tcccgcccgc ggcgctattt gaattttacc tccataaaat tgatgagcac
    15481 cttctgacaa cttctgaaaa aacaatgtct ttgcttgagc gatttgttct ggcgtaaaat
    15541 tctccggaaa aacgttatca agattgctga gatcgagttt caattgtttc ataaatatta
    15601 tctcctgtaa aatttattcc tgacgataac gagcaactcc gacgcggaaa agatcattac
    15661 cgtttacatc aatagccaca aaaactggaa aattaactac cgtaagttcg tagatagctt
    15721 ctggaccaag ttcctcaaaa gcaacgatac gagccgtttt tacggcttgt gccgccacga
    15781 ctgccgcgcc tcccacagcg acgaagtaaa ccgcctggta gcgaataatg gcatcaatca
    15841 cgttctgact gcgttcgcct ttaccaatcg tagccttcac tccggctgac agtagcggcg
    15901 gagtgaattt atccattcga tacgaactgg ttggaccgac cgatccaatc ggattaccgg
    15961 gccgagccgg cgccggtccg gcataataaa tcaccgtccc cttgatatca aacggcagag
    16021 gctggccaat ttccataagt ttcaccaaac gttgatgagc aacatcgcgg gcggtataaa
    16081 tttttcccga tattaaaact ttatcaccga tattcagttt tatcaaatct gtgttttgaa
    16141 ggggagtgga taaatggcaa atatctgaca tgttgatttt actcaattat agcggtttca
    16201 tgacgatgac aatgacaatc gagattgact gccactggca tagaagctat atgacagggt
    16261 ttataaatga tgctaaccgc ccaggtagtc gtacttccac ccaatccctg tggcccaatt
    16321 cccagccgat tgattctttc caccaattcc tgttcgacat cagcccaaaa tggctcagga
    16381 tttttctcgc ttaatggtcg taataaagct tttttggcta ataaagccga ctgctcaaaa
    16441 ttgccaccca gtccaactcc aacgacaact ggcggacatg gccgcccacc ggcgcgacgt
    16501 acacaatcca caataaaatc cttgacactt tccaaaccat cggaagcgtt catcatccgc
    16561 acttcggaca tattttcgga gccaccgcct ttgggcgcga cggtaatttt caaagaagaa
    16621 ccaggcacaa tttcggtata aataatcgcc ggagtgttat ccccggtatt ttctcgtttg
    16681 acaaccggat cgctaaccat cgccttgcgc aaataacctt ctgaatatcc ttgccgtatc
    16741 ccttcattaa tcgcctgctc gaaatcgcct ccaattagat gaacatcctg accaagttcg
    16801 acaaatacta cagccactcc ggtatcctga caaagcggca tctgccattg cgcggccagt
    16861 tcgtaatttt ggattaatat ctggaaaatt tcacgaccga tcggcgattt ctcactttgg
    16921 agcgaagcct gggctttcgc caatacatcg gcggaaacgt aataattggc gtcaatgcat
    16981 aactttttaa cgacggggat tatctgggcg acatcaatat ttttcacagg atatttccac
    17041 ttatccaacg gttggatata attttcaaag cgcaaaataa aagtaattta ttcatcagat
    17101 aaaacgtaaa taacatcctt catatatcta atatccaaat cacaatttac ataaaatcta
    17161 ggcaaaatct tttctcctta cgaacggaaa ataaaataca ccgcacccaa aatacagaga
    17221 ccagcataaa aatagtcggt tttcagtggc tgtttcatat aaaaaactgc aaaggggata
    17281 aagaccgtga gcgtcacggc ttcttgcaaa attttcaatt gcgccagcga catttgggta
    17341 aagcccaaac gattggcggg aacttgaatc aaatattcaa acaacgcaat tccccagctg
    17401 accaaagcgg ctacatacca ggctttgtca cttaagttac gaagatggcc ataccaggca
    17461 aaagtcataa acaaattcga aaataccagc atcacagcgg ttcgcaaaac gactgataac
    17521 tgcattgaca tacctcagtt ctattctttt ctttatttaa tttaatctct agctaagtac
    17581 ttctcagttc cggtgattgc gacgatttcg ccgaaataaa ttcgatggta atcgtgtcca
    17641 ttataatttt tagcaatatc gtccttgaga aaatggctgg gctccatatc actccagtat
    17701 attttgcgac actcgatttg caattcggct tcctgaaaaa ccggcgccgc cacgatcctc
    17761 gccggacaag gcgtaatacc gcttgctttg actttgtcag tccggcgacc agaagtcgaa
    17821 ccgattattt gtaatttttt gtggtactca gccgcaaaag cgcaaagtgt aaaggtttca
    17881 tacttttcca taaactcata agtataacgg gtcggacgga cgacaacctg cgcaaatggt
    17941 agagaccaca ttgttcccag actgccccaa cctaccgtca taaaattaaa gtgtccggct
    18001 ttgaaatcac cactggcgag tactagattt tgttcatccc agagggcatg agtttttaca
    18061 attaattttt caaacggaat cgttaacatt ttcttggtct cctttgagtt ttctgtcatt
    18121 attctaatgg tgaaagttta taaattctta aaaactgagg gctcggcatt ttcaattcat
    18181 cttattgtta tcgttaaata atggaattag tccttgttaa tcttcttaat atcttccatt
    18241 ttaaagctga tgttaattcg gccgcgcggt tgtttgatag taacagtatc ttgttcgaga
    18301 attattgtta gggaaaacgg tgaacttgtt aagcctttaa tatcaaccgg agactctttt
    18361 gtctcgcgtt tctcgcacca gagcgtaacc agattattcc cgaattttaa attgcgaatg
    18421 ccatgtttat ttaacagcca caagcgccag gtcagttggt cgtccggtcc atttacgtta
    18481 aatcccagta catcttcgat cagcatagcg accggtccaa atccggaagt cggaatataa
    18541 tttgggcgac agagattata agtatcccag cgcgtaccgg gttgatcgtt ttccggtgaa
    18601 tataattccc acactgtggc attacagttc gcccagtatt tttcatcaat caaacttgta
    18661 tcaggaaaat atccagtgaa aattttttcc atatttgtga tatgattcca ggcggtagta
    18721 gtggcaaatt catattcgcc ataccttttc aatcccgcga taatcatata attttcattg
    18781 ccccagacgc caccccgcca gtaagcacca ttaggtttga agccatcttc atcagcagat
    18841 aatgacggaa agagatgccg ccgggcaaat tcatcatcat ttttcaaatg attaattagt
    18901 tcaggagcgt cttcgctgga tggaatgccg gcaattagcg gccaaaatcc agcggtcgtc
    18961 tttattctaa ttgggtgacc gtcactggtc aaatcataat aaaatccatc ctcatcgtgc
    19021 cagagtttct cgcgaatgac ttcggtgatc ttttgatagc gattttgata ataaatcact
    19081 cgggtatctt ccccgatgat ttcagcagtt tcggctaatt gttctgcaaa aagagccatt
    19141 tggcacgaca tatctgccca tcccccaaaa tcattatcgg tgcgtggaga attgatcatc
    19201 agagaaccat tctggctgtt ataatatagc ccgatcgctt cgcccttagc ctgacagaaa
    19261 ttatccagcc attcaaaata tttttcccag atcttaaaat actttcgcaa ccgactttga
    19321 tcaccagtca ggtaataata tttcaattct ggccaggtga aaagaggcgg attaatcatc
    19381 ggttctatcg cactgggagc atgcaaatac tcaccggtca tcgagttgta tatccgcgaa
    19441 ataaatccat ctgagcgttg gccacggtaa aaattatcaa gggactgcat taccggaaaa
    19501 tgggaactac cataaacacc aaaaagagcc agcataatcg ttgaactttg attgattagc
    19561 aactcacttt cacaattgac ataatgataa ctgaagttat tttcacgatt gccacgtttt
    19621 atattttgct ccattatttg ccagattttc caatacatat tcctgatgcc ctggtaatcc
    19681 ggaaggattg gatccggtat ctggttttgg tatagatcgg aacaactatt tagactgatt
    19741 aaaaaaatca gaatagtaaa gaaactcgat ttcagcgcgg tggcggggga gtttctctga
    19801 gcatattttc ttcctgccat aagttcttta gtggttcatt taaataatta gcgtaggttg
    19861 cgcgggcttt ttcagacata ccggctccgg cataagcttt agctaacact aaacgataac
    19921 gtttaatgtc tggattatat tgaacagcct tttctaaata tttgacagca ttcttataat
    19981 ctttattttg aaataataat tttccatgat aaaaataagt ctgcggatgt tcaggctgaa
    20041 gttcaatcaa acgagctgaa tacttcaagg ctccgtgaat gtcacctgct cttcgtttgg
    20101 cttccgccat tatataaagc gcatcacaat tattgcgctc cagtttcgta atcgcttttg
    20161 cataatacat tgccttgctc ggttcattca ggtaaaaagt atatattcga gctagcgata
    20221 atagtgtggg aattcggtaa tcccgattta gtgctttttt ataataagta gccgcgtttt
    20281 cataatgccc ctgttgaaat tcaaggaatc cccaggcttc taatgtctga gctgaacgtg
    20341 gtgctattct cgacatcttt tcgcagactt tggcgctttc gacgaaatag ccttcctggg
    20401 ctaaaatcgt aactaatagt ttaagctgaa aagggtcata ggtaatctgg acggcatcgc
    20461 gcgccactcg gacagcttca gccttttgtc cacagtcata taatatcgaa gcgtaatata
    20521 cttgagcatt agctcgtaaa ggatagcgtc tggctatttt ctccaaactt ttcaaagcgg
    20581 ctgactgttg ttttaaccga gcggcggttt cgattaaaat ggacaaagct tcgtcgtctt
    20641 ttgtcacggt gtagagtttg gtgcaaacct tttcaatttt gttccaatcc tgactattgt
    20701 attgcagaaa aatggcgtaa tcgtatagaa agccttctaa ggaatcgttg agagtaaaag
    20761 ccgtggcata gaatttttcg gcatctgaga ctctcttttc ctggcgagca acacaagccc
    20821 gtccccaatt accgtagaat ccctcaccgc gctggattgt ttcaatataa gattgatcag
    20881 cttgaaaata tttctttttt tcataatatt tttcagccgc tttgaacgct ggccgcgtct
    20941 cgggtctgat attttttttg atctgccggt aatgcaatcc gtcaggaagt ttcgtaaaat
    21001 cgtctgacgc cgaaaaggca agccctgcga acaaaagtaa aagtaagctg acaacatact
    21061 ttctcacggc ggaaaatatg ccgaatttcc gagcaaattc caaattaaaa tatctgaacc
    21121 tttataattt cactaccttg aactgcattg atctttttca atagctggtt atttatttaa
    21181 acttttaaat taaataactc ggtccgctac gattaaactc attaatgaat tcaccgaacc
    21241 gattaccgat tgattcatat ccagaataac ttttgaatta acggtatatg tgattaattt
    21301 ccgattgttt tcgtcgttgg tttgagcggc gaaaagtcca agcgagtttt ttttaataag
    21361 cagttgaaaa acgaatgact atgcgccaga tcctctattc gtcaaaattt ttctctaatc
    21421 cctttcagaa atgcgataag ttttcagcca gttcaggaca caatttacaa ttaaaagcaa
    21481 caaaccaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gcaataaact
    21601 aggggcttca tttctcggct tctttgaaat ctcgatctgg ctcttagcca tttcacaggt
    21661 tatgcaacaa ggcttaaata acgtcgctag ctttttcgct tacggcttgg gtttcgcatg
    21721 cggtaacttt attggcatta cgattgagga aaaattagcc ctcggcttgc aaactattcg
    21781 cctaatcagt accgaaacgg ttgatatttt gacgatgaca ctacgagacg ccggatatgg
    21841 agtgactgtg attaatgcct caggcgctaa aggtccagtt cacattttat atgtaatcgt
    21901 tcagcgtaaa aaagtcaaag atgttattag gattgcaagc gaaattactc cggacatttt
    21961 tatttcggtg gaggatattc gttcggtaaa agccgggttc tttccggaaa ccaatcggaa
    22021 ttttaaatgg tggttttgga ataaaaataa ataacaaccg ataattacgc ttgtaacatt
    22081 ttgggagtat aatccatgaa attctaccgt aaatcgctgt ttatcgctta cttgatcggt
    22141 ttcattttat taggaattga tttcctgacc aaaagtatcg ccaatcttcg gttacccttt
    22201 caggaaaggg ttgataccgc gttgccgttt ctatcatggt ttctcactta caacaccggc
    22261 tatcatttta tatttggcaa tatttccaat caaaaaatct gggctatatt cggaattgca
    22321 ctcgtcgcgg ttttaggctt ttccctgtca cgtagtttaa ttaaggaaaa ggaatcgaaa
    22381 gccaatgtca tcattcttac gattatttta agcctgataa taggttcggc tggcaacgtc
    22441 ctcgaagtct tgattaaagg tcatgcgacc gatttcttcc tctttcatcc ttttccctgg
    22501 ccgagcaacc tttgtgatca atacatcaac gccattattt acattatgtt accaatcacg
    22561 attatcaaat caattctcga ctggcgtcaa agcaagaaga aaatggcaca gaattgatcg
    22621 gagtagctac aacacatcga gaaagttatt agttctcttc ggataatttt taatattgga
    22681 ttattatgaa gtttgggtta taaccgacga gcaatttcct cacccaacca tcaatttgtg
    22741 gataatggct ttctgaacat gcatgcgatt ttcggcttca tcgaagacga tagaattcgg
    22801 gccatcaata acttcatcgc tgatttcttc gccgcgatgg gctggcaggc aatgcattat
    22861 aagcacattc ggcttagcgg cattaactaa ctgcagattt acctggaatc cggcaaagtc
    22921 gcacttacgc ttttcctttt cggtttcgcg tcccatactt gcccagacat cagtatatat
    22981 cacatcggca ttttggaccg cttcaaacgg ctcgtgagat atttctacct tgcttagtcc
    23041 gctattttga gctttggtca gcaattcctt gtccggttcg taaccgggag ggcaagccag
    23101 aacaaaatgc atgggaagtc tcaaagtcag ttccagccag gaattgacaa cattattacc
    23161 gtcgccgacg aaagtgactt tgagattatc aagattgcca cgtttttcca gcacagtaaa
    23221 aatatcagcc attacctgac aggggtgatt gtaatcggtc aagccattga taaccggcac
    23281 agatgaaaat tcggctaatt cgagaatatg ggcatgcgca aaaagtcgcg ccataattat
    23341 atcatcatag cgagccacaa cttgggcaat atctttgacg gcttcccgtt ggccaaggcc
    23401 gatgtcattc ggccctaaat aaatggcgtg cccacctagc tgagtcatac cagtctcgaa
    23461 agaaatgcgc gtgcgagcgc tcggtttggc aaaaatcatc gccatagttt tgcccttgaa
    23521 cggagtgtac tcccaacctt ctttgaggta gcttttgagc tttttcgtca gttcaaacac
    23581 ttcccatatt tctgccgtcg aaaaatcagt aatatgtaaa aaatcccttt tcattaaatc
    23641 cctcaggtga atcaataaat taaagtatat aacgactcaa atcgcggtca cgcactatat
    23701 tttgtaatct tgcttggaca aactcgcgcg taatgtgtac ttcccgaata tctttatcag
    23761 gaaccttata gaggatttct tccagtaagg ttgtcagaat cgtatgcaga cggcgtgcgc
    23821 cgatattttc cattttctca ttgacttccg ccgcaatttg ggcaatttcc tccactgcat
    23881 cgtcgtcgaa aattaagtca accttctcag ttttcagtag agcggtatat tgtttgagca
    23941 aagcgttacg cggatttttc aaaattttga cgaattcatc tttgccgagg ctatccattt
    24001 caactcggat gggaaagcgc ccctgtaatt ctggaatcaa atcggaaggt ttggtagcat
    24061 ggaaagcccc agccgcgata aataaaatat gatcggtgcg aaccactccg tatttggtct
    24121 ggacattact gccttcgacg atgggtagaa tatcgcgctg gacaccttcg cgcgaaacat
    24181 ccggaccgta gccacgctga tcgccgctgg atgcgatttt atcaatttca tcaatgaaaa
    24241 cgattccggt ttcttcgact cgctgaaccg cttcccgaac taccgtttcc atatcaatta
    24301 atttttgtgc ttcttcctgg gtaaaaattt tacgggcttc ggaaatggtt actttctgga
    24361 tttttttctt ctgcgggaag gcactaccaa gcatttcgga gagattcatc tccatctcct
    24421 cgataccggc tggaccaaaa atctgcatca tcggcatcgc tccctgattc accgtgatct
    24481 cgatcaggcg gtcttccatt tcaccattct caagcatcac gcgcaatttt tcacgagtcc
    24541 gattgaattt ttcatcacct ctagggataa tttcttcttg ctccgaatgg tgctttgtct
    24601 cccctgaagg aagtagaata tcgagaatcc gttcgttagc cagaaaaaga gcttgactat
    24661 tgacttccgc catacgttca ttctttacca tagtcacggc gataccagtt agatcacgaa
    24721 tgattgactc gacatctcgt ccgacgtaac cgacttcggt aaatttggat gcctcgactt
    24781 tgatgaaagg tgctccggct aaattagaaa gtcgacgagc gatttcagtc ttgccgatgc
    24841 cggtcggacc aatcaggata atattgtttg gtaaaatatc ctcctttatg gcgcccctca
    24901 cgtgttggcg ccgccagcga tttcgcatgg cgatggcaac agcttttttg gctttttcct
    24961 gaccgattat gtaggcatcg agttcgcgaa cgatttcgcg cggtgtaagt tctctatttt
    25021 tcataattct ataattgtaa aattattgtt ggtgtaaata caaatctcgg aagcgattcg
    25081 gagtgattca gccacgattt cccgggcatt catctccgta tggcgtaaaa gggcgcgggc
    25141 ggcggcggta gcaaaggcgc cacccgaacc tattgcaata atatcatcgt cgggctcaat
    25201 cacatcaccc gttccggaaa taattaacat ggtttccttg ttcattacgg ttaaaagagc
    25261 ttctaactgc cgtagatatt tatcggtgcg ccagatttta gccaactcga cggccgcacg
    25321 gtttacatta cctttatatt tttctaattc attttcgaat ttatcgaaaa gggccatagc
    25381 gtccgccgat gctccggcaa aaccgaccac tacctgatca ttgaaaattc gacggacttt
    25441 cttggtcgta tgtttcataa tcgtctcgcc gatcgtcact tgaccgtcgc tccccatagc
    25501 ggcttggcca ttacgacgca ctccaataac ggtagttgct attattctgg gtattggttt
    25561 ttcctgcata ttatctccat tttactcaat tatccgaaca aaatttacac tttttagatc
    25621 aaaatctacc agctgaatac ccaattgcag agaatccagc gtagcagttg cattttttaa
    25681 aatcccggcg atgatggctg gtcgccgacc tttcacattg aacaatgtcc tggattgagg
    25741 gactttcaga atgtctttaa tctcggtaat gtccatccgg cggctactgc ttcgaataag
    25801 ctccgcaaga gtatagcggg cgacttcgtc ccgcagagaa ccatccaata aatcagttag
    25861 gcgattttcg gcttccggaa ttgttctgac tctttcataa aacaacagcg gatcggatat
    25921 tttccaatag gcaaaggact cgatggcgat gaatttgtca tctttagtag gcatctcaat
    25981 tacgttgctc tgccattcga tcaagttgtt ggggtaaata taggttttcg ctaaaaacgg
    26041 tattattaga tgaacaccgg ctgttgttac cggcgttaca gtcggttgag taaagcgagc
    26101 cacgatcatt tgctcgtcct cgtgcaaaat taccgtcgct ctctccgccc caataataat
    26161 cagcgctaga atcaataacc agataaatga cttaaacctc atccgagacc tccgtgagat
    26221 aatttcttgt agaaatcagg cgcattttgg ctgttttatt tcactcctac cagttttcac
    26281 caattttttt gactgaatcg gcttcagttg aatcggtttg attaaacggc tttagtgata
    26341 tagttgtatc cggttcggat agcggtaaag ccggtcgcgc ttctttcaac tgcacgtgct
    26401 gaatgacaat tccagtttta taaagatcaa ttttcgtctg catataattt ttagctcgct
    26461 cggcgactgc tcgtcgctca gtctggaata cttcgcgaaa ggaaaagtca ccaactgccg
    26521 aacgcatagc cgccgcgccg actaatcgta acatattctc cggattttca actccaaata
    26581 aataggctac cgggtcttga atgctgtact gaaccaccca ttgcaaatca accattttca
    26641 agtcaccggt tagcaaacgc gcttccggta gatagaaatt atccgacaga gcagtcagac
    26701 tatcggaggt agtccgaata ccaaattcgg cagtgtaagg gtaattaact tttacccgat
    26761 ataggcgatc aatgccccaa ggtaatttcc aatgagcacc aggtccattg atctggtgaa
    26821 ctttcccgca gcgagttact acaccggttt cgccgggctc aattgtataa aatgacatca
    26881 aagccgtaac aattactacc aacactaacg caatcagaat tatcaaccag taggaaacgc
    26941 tcgaaacctc gatttcttta ttactgaatt gaactttttt accagccatt tttcctcctg
    27001 ataatttcat aagaaaaaca tcaaaccacg cgtcgcaagc gagcaaccgg aattttcatt
    27061 tgctcacggt atttagcgac tgtgcgacgg gcaactaaat aaccgttttg cttgagtaaa
    27121 ttggataact gctcatcact ataaggttta tgtttgtctt cattatcaat aatttccttc
    27181 agcttttcct tgactatccg agtggaaatt tcctcaccgt cttcgtcttt aataccttcg
    27241 ttgaaaaagt attttaactc ataaatgcct gagggcaact ggacatattt attattagtg
    27301 actcggctga tagttgagat atccatattg acatcatcgg caatgtcttt tagaatcatt
    27361 ggcttcagcg gtttactggg atcttcgaag aaatccaatt gtcgggcaat gatggctttc
    27421 ataacccgaa tcatagtcag gcgtctctgc tgaattgctt gaataaacca cttggcgtta
    27481 tcaattttgc gcttcaaata attcttggtc tcattgtcaa ttttagaacc attgctaatc
    27541 atctgcaaat aggtggaact aatttgcaat tccgggatgc gcgtatcatt gacttcgaca
    27601 atatactctc catctacttt ttcgacgatc aaatcaggga cgatatattg cggatcgagc
    27661 gtgttcaaag tcgcacccgg tttgggattg agtttggtta tcatctcctg aacggcttgc
    27721 attcgttcct tgtcccagcc gtaggctttc ataatatttt gatagcgatg attggcaaaa
    27781 tcctcgaaat gctcactaat gactttgatg gcatcatctg aattcggtat ttgattaagc
    27841 tggatcagaa ggcactcttt caaatcgcga gcggctaaac cgggcggatc gagttcctgg
    27901 atttctttca gcactttcag cacttttttc tggggcaatc ccagtccgaa cgcgatattt
    27961 tctatcggca gggtaaggta accattctca tcaagattcc agacgatttc agccgcaatc
    28021 cgttcttctt catcatcaag attatgtaat ttcaactgat cgaatatatg ttcaaccaat
    28081 cctctcggtg ctggctgaac aaattgcgtt tttttctggt cggaatctct ttcttctttg
    28141 ggtgtataat cgtcgttttg cggaagaatt tcctcccaat caatatcatc ttcttgggaa
    28201 gtttcctgat tatcctgcaa ttcttcttcg gaactttcac taacctcagg atttacttct
    28261 tccaaaaccg gattctgttc aagttcctga agtacgcggg cttcgagagc taaggtattc
    28321 aattgcaaaa tcgtagattg cagaatttgc tggggcgtga gccgcaattc ctgagtaagt
    28381 ttctgaacct gggattgact taactgcatc atctggcgag cttgaatgct tctcctaaat
    28441 ataagcggcg ggcatcttca tcgttagcta aaaattcaga tgtccccgat ttaagaatac
    28501 gaccttcaaa cattaagtag gaacggttag taatcgaaag cgtctcgtgc acgttgtgat
    28561 cagttatcaa tacgccaatg tcccgatctt ttaagctgac aacgattttt tgaatatctt
    28621 ccactgcaat cggatcaacg ccgacaaatg gttcgtccag tagtataaaa tccgggttgg
    28681 taatcaaggc gcgggtaatt tccgtgcggc gtcgttcgcc accggagagg gtgtccgccc
    28741 gtcttttgcg taacttgcga agctcgagct ggtcgagcag gatttcgagt cgttccttct
    28801 gctcctttcg gctaatgccc agtgtttgaa gtaccagcat caaattttgc tcgacggtta
    28861 atttacggaa aaccgatggc tcttgtgaga gataaccaat tccaagttgg gcgcgtttgt
    28921 acatcggcaa acgcgtgatt tcctgttcat taatgaatac ttttcccgaa gtcggcttga
    28981 tcataccggt aaacatatga aatgtagtcg ttttcccggc accgttcggt ccgagcaagc
    29041 cgaccacttc gcccttggac acttcgatat tgacggaatc aacgacccgg cgtttgccgt
    29101 atatttttac caggttttca cctcgcagag tttttttttg cggttggtta tttaattcca
    29161 tattttagta tttcagctcc cggctggtta ctccagaagg tttgaaaatc acccagtttt
    29221 tcaaatccga ttcggattca aatccaattc cggttagcga atcttcggga gtcacaaagg
    29281 ttacaaaaac ttctgaaata attcgctctc ttcggtgatc ccagcgtaat tcctcggtga
    29341 aaagggtaaa gccactatca gaaacgacta caacattacc tttggcaatt agatcgttcg
    29401 tgtgttcatt aacagtcgct gtctgagaag tcaagtgcga taaatgatgt ccctcactat
    29461 catagaaatc tactgccacg tcggtatcca tatatatcaa cgcctgatca ttattcttcg
    29521 tcaaatggcc agaatgaaca accgctcgtt tttggccatc tttagtcagg gtaatattcg
    29581 aattccaaat ttcttgatcc gggtattgcc ggtcaatcat ggtaggctca taactcttat
    29641 cttctgtgca accccagcaa aataggacaa caaataaggt tgatattaaa aatcttccct
    29701 tcaaggcata atcccgtagc gcaaaatatc atgaatatga ataattccga tcggtttgcg
    29761 attttgctcg ccattatata cgaaaaggtt ggtgatcgaa tgttcttcca ttagatgcaa
    29821 ggcttcaatc gctaaactac tttttttaat ccatttcgga ttatgggaca taacttttat
    29881 ggcagtttgt tccataaaat ccttctgcct ttcgagaccg cggcgcaagt cgccgtcagt
    29941 gacaacacca atcaggtttt cggaatcgtc caggacgccg acaatgccga ggcttttttt
    30001 cgtcatcata taaagcacct caggcatttt gtcgctggac ttgacaaacg gtatttcttc
    30061 accggtgtgc atcaaatcgg ctactgtagt caagagtctg cgtcccagtg aaccgctggg
    30121 atgtaaaccg gcgaaatcgc gccgattaaa atgacgtttc gcgagcaaac taatcgccag
    30181 cgcatcaccc atagctaagg ttgcaatcgc actggtagtc ggtaccaatt gtaatggatc
    30241 tgcttcacca tttatttgag tactaatgac atagtcacat ttttttgcca gagttgattc
    30301 attacgcccc aataagccaa tgatgggaat gcgcattttt ttcagcggtg gaattaattg
//
LOCUS       DAOOMU010000016        29286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1389_length_29286_cov_22.996647, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000016 DAOOMU010000000
VERSION     DAOOMU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 29286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29286
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1389_length_29286_cov_22.996647"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1633)
                     /locus_tag="PLI55_02750"
     CDS             complement(<1..1633)
                     /locus_tag="PLI55_02750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HPA99787.1"
                     /translation="MKTSHTRAFLCILIAFLMVTVSLTAAGNIATYNGKEIRSNRIVV
                     VNNSILKKLPAKPDQTMRLESARQAYQGRIVRNLPTEGIAEWEIKGDMRKTLDALNAI
                     DGISAFPNFVFKRENLQPKPIQKDALVPNDYFISLQWALNNDGTFDSLYTSMGGMPVV
                     DAVAGADISAFDAWDITTGGTYTIGEVTSDVVVAVYDDGIDIYHEDLQDNIWVNPGED
                     LNGNGIVDDGEWNGIDDDGNGFVDDFWGWNVMNDDNSYLNSGSYHGTHVAGIIGAEGN
                     NELGIAGVNHHIKMLSVLIWDDYGSTDAITLMYGYYYLSTLLKSGVKIVAVNQSWGGG
                     AYLNDRDEQRFVDVMTKYAREHDSYGMIWVCSAGNDDVNTDKLEYYAYPRLIQSPNVI
                     SVGSTDYADNKSDFSNYGQATVDIGAPGTDIASCYPYNSYVYMSGTSMAAPHVTGVIA
                     LAKSVFPEDDAHDLIARTLATADPVTNLRGLWNSEGRLNAHLAVDPTVIGGFKRSVPT
                     AAYIQKNFTDDYGVATVGFVNGKDIDVSVTSATISG"
     gene            1814..2614
                     /locus_tag="PLI55_02755"
     CDS             1814..2614
                     /locus_tag="PLI55_02755"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA99788.1"
                     /translation="MSNIQEIKVRSILSQSKLPGVDFVINPYRGCQHACVYCYACFMQ
                     KFDGHSEPWGTYVDVKSNALEILTRQLAKCHNRTVYLSSVCDPYQPVEAEYQLTRQIL
                     KHLIIAQPQLTVQTKSALVIRDLDLLEKFKNCSVGMTIVTTDENLRREIEPGASPIAA
                     RIDALKQLKAAGLETYVFVGPILPYLTGWKQIVLQTREFIDYYMFDHTNPSGRIWSHI
                     KSWLIAKHPELLSSYEKILFGRSDYWWRVSRAIQDFCDKENINHQIFF"
     gene            complement(2748..5756)
                     /locus_tag="PLI55_02760"
     CDS             complement(2748..5756)
                     /locus_tag="PLI55_02760"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99789.1"
                     /translation="MKSAIHYSLTDLLNRASDLGNHHRKFIHNIEMNFDNLFALINNE
                     HLTDQFHLLRFSPHPAREMKLIADLVANRETLLEMLGCYFSIQLLLLNFKAIDVLKMK
                     LSTTTNHYAVYRSFLLRTGNDFRLLTAAYMNTLLDIFLPRAQRPDFVICGVGTRVDQD
                     DIDLGIVDTGPANRAILTNAFGAMSNEMLKYACSLHFHLSEHVGTKGYTASIEDYHNL
                     LDHQIQDFVLISEMVNAVPILGNRELFDRFKNEILDRYYFSADRDNKYHEGFLRGLLG
                     EIRDLMYKEPPENLLNPKRDGLRMLKALIFALKTWKRVEGNTSLEVLDILLVKERFNH
                     ENYYQVFQALTFLETFRFLYQLFVVQEEEIHLDSDEMQLSLQKVAYAMGYQDHSYALA
                     CTQMLTHYYDHHKIARLGTERLVEGLTEHLRQITIFYPITHFQSRAESKAPLPDNIPL
                     NFMQTARFFQGVRFWDDLLSSLDNPNQILLKRFLADFMKLSEARRAVLMKAYVKWGVK
                     TPYTMISLFSIIADHQPKIVETQFFQEFIRDFIYNLEPTLENVSRLCQILNLAPQMMN
                     NFLSILPVELLTRLIAVVEEPYWKPEITSLRDTLLMLCRIYRSSSYYFKRFVQRAFNT
                     YAEYITSLTNQPKLNQLAEGLFRNIDNFDSLNEKITRLGDYYDFEFMRLGIALLDGAP
                     FDKINREFTNFSDNYLQMLFELCQEEVAAADQKEIPEMHDLLAIFTAGGHARGQAFDD
                     DYDLIVLLNSDNPAILDFGNKVIQKMNKYILRRSIIPQFRFADRFRRFVTTFSDLRRL
                     FERPDENTFIDQSQLLGARLVIGSDYFLKVFQSEIITPFIFQRKAEFIEAMLNEMRSR
                     HNYYNSTKIEVPEIKEGPGGLRDFENFIFILKAYFEWAEPLTETLFSRIESAITNDDS
                     NFIALVRDYQLLKHIRDLYRLSVSNDDHLDKAYLDQLVRPFCLAHGLELENGAELAEF
                     IQRIMRKNVERINLLLRTLELPEIGALPGK"
     gene            complement(5781..6341)
                     /locus_tag="PLI55_02765"
     CDS             complement(5781..6341)
                     /locus_tag="PLI55_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPA99790.1"
                     /translation="MSFHYEIRLSGAGGQGLILVGKVLAEAAAIYDGLNATQSQSYGP
                     EARGGASRSEVIISDGDIDYPKAENIDLLLALTQEACDKYIADLKPDGILIVDSQMVT
                     KIPDGNFKVVALPIIQSAVERLGKFVVSNIIALGVIYKLAGIVSEDAIKNAIRARVPK
                     GTEELNLKAFQTGIELAEEWLKEHAR"
     gene            complement(6353..7168)
                     /locus_tag="PLI55_02770"
     CDS             complement(6353..7168)
                     /locus_tag="PLI55_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPA99791.1"
                     /translation="MDYLYYLRTKNLPHIWCDGCGNGIVLKSLLRVINKLGWDKNDIA
                     LVSGIGCSSRTPGYVDFNTLHTTHGRALAFATGLKIANPKLHVIVVSGDGDATAIGGN
                     HFIHAARRNIDITMIIFNNMIYGMTGGQVSPTTPAGKMATTAPYGNPDPPFDISRLAA
                     AAGASYVARSTVFHYARLDKYIEQALTRKGFAVVEVLTPCPTSYGRMNKEGNGAAMLL
                     YQKENTVSLAKASEMSPEELRNKLVVGVFVDRQQPDYQSHYEAVVTKARSEIK"
     gene            complement(7177..>7605)
                     /locus_tag="PLI55_02775"
     CDS             complement(7177..>7605)
                     /locus_tag="PLI55_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPA99792.1"
                     /translation="DETGSPTNDPAQIEQQLLRINRKIERYRDQIVEVEEFETQDMEI
                     LVIAYGATARSAKKAVSLAREAGLKAGLLKLKTLWPFADIEIEALAKSVNKIIVPELN
                     LGQIAHEVEWTVRRAKPVIRVNRINGEPITPAEILAAIKN"
     assembly_gap    7606..7705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7706..8019)
                     /locus_tag="PLI55_02780"
     CDS             complement(<7706..8019)
                     /locus_tag="PLI55_02780"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPA99793.1"
                     /translation="MNKSNASSSNSRIFMMQGNTACAEGAIAAGCRFFAGYPITPSTE
                     IAEVLARRLPQIGGNFIQMEDEIAAMGAVIGASLAGVKSMTATSGPGFSLKQENIGFA
                     IMA"
     gene            complement(8016..8231)
                     /locus_tag="PLI55_02785"
     CDS             complement(8016..8231)
                     /locus_tag="PLI55_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPA99794.1"
                     /translation="MTKPEVQINPEWCKSCEICVKFCPRNVLEMGRFTAQVVHPENCN
                     GCKICESLCPDFAIVVTVPEKAKEEAK"
     gene            complement(8363..9292)
                     /locus_tag="PLI55_02790"
     CDS             complement(8363..9292)
                     /locus_tag="PLI55_02790"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPA99795.1"
                     /translation="MPYLIPEPLKTGGTIGVIAPSSPAPRQTTALGLQYLQNLGYKVK
                     SASNLNRGKFFLAGSDAERLKYLEQFLCDPEIDALFCVRGGYGLIRIIDQLNYQRLAK
                     IKPKVLVGYSDITILQMALAAKLGWVTYSGPMVSTDLGQSLSSFSAEWLWRMITSNPY
                     PIELTNPLAQPLQAFQPGSAEGVLIGGCLSLITPLLGTAFMPDLKGAILIIEDVDEKT
                     YRLDKQLQILRLHGVFDQIAGLIVGQFVNCFPSNPKRSFTLEDYLSNALKDTHFPVIT
                     NCAYGHLKQRFTIPIGIRARISTNPPRLTLLGL"
     gene            9477..11093
                     /locus_tag="PLI55_02795"
     CDS             9477..11093
                     /locus_tag="PLI55_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPA99796.1"
                     /translation="MKLSQKSIINLLTFACLLLLNESGLTQDKELIDKVSGRMEYDFQ
                     QMTEESAVGDVNTLRQFIADSLKFVYGERYQQELDSLESIYMTTIKTLETECTRLNNL
                     NKILIDSLEKARSARPKSVIETKDLDPTFESRYFQYGKILRLEEPRHKTGFLKMPTVI
                     ENIAPFQIQELNEYYDRYFPIARSDSVLDFIIQINIREKDWGNAGCNIIKFLYLFPNS
                     ALGEEIKTVRAGIFRTEKYYQPYSAFLTDLLNKIPQLPLADQRYYHYVELLKGFPDQA
                     IRNNFIPEAYKFLELYPNSKYAAQANLWIAEALLANNQPHSAYLTLQKIMILYPSDEL
                     YCRALHTSGVIQQEQFNEYKNAVQTFTDLIKRFPQDTLAENAHYRIAKISDEYLNDWE
                     RAVEQYQIYADKYPQSPNAIPALMRKAKIQSTQMNLIEEAVRTYKSIDERYPSTTGAF
                     NALTEAGNLYMTKGRYDQAIEIYMSIFQKYPQSENAVAALEKVYNIYQTKIKDNEKSI
                     EILNLIINNYPDTKASARATKQLRKLEKAR"
     gene            11165..13579
                     /locus_tag="PLI55_02800"
     CDS             11165..13579
                     /locus_tag="PLI55_02800"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPA99797.1"
                     /translation="MINGIISIIVLSLTTTSLPAQTITRPKIGLALSGGGAMGFGHIG
                     TLKLLDSLSIPVDYIAGTSMGGIIAALYAVGYTGAEIEEIARNVDWADLFNDAPSRQA
                     LPYFQKQEADKYQFELGIRDLRPVDKGGVIAGQKITLYMTRLVLPFLTVSDFDSLIIP
                     YRCVAVDLTTGSEVVIDHGSLPIAMRATMAIPSIFTPVVWGDSLLVDGGLLNNLPVNT
                     VRKMGADIVIASTVRSPFKTRDELRTTLDILSQTSNILRDNKLEAEAKNADLLINCQL
                     EKLGAADFSTAKVCKIIEAGEAAAHANLDELLDLKKKYNLSRYGPENINDLNSPSGYR
                     VARLIIKGSQTIPVDVVRSTIGIKVGELYSPETLAAGVERLKSFDNYIRVRPTVEILS
                     DSTVEIKINLVEKVKAQINNIEVRGNEYLTDDFIRRSFGIIPGEVFSMERIESQINYL
                     YGLDYFNNISYTTEPAGEGKINLILNVVEHTPQKLRLGVRYDDFHYLVAALNFQTTSN
                     IVHGLRLDVEWQFIGLNQIKLKTLYPSRRFQLPFYPFVNAHYREIPTYVYSINGNRIA
                     SYIDRSALFGGGFGLLYKNYWNFEAELDYESINIKPDIAPDEPLRFYDWNDDLYKFQL
                     SSNIDLLDNAFMPRKGALVHCVYEKTISQIGRHTNYRRFELSADVYQSIKRNTLHFSG
                     FYGNAKMDGSTNRFIYRGGQETFVGVEYDQLVGSEMTILRLDYAYNLLHNLQAKAIVN
                     TAIGFRNIYHETIKESQNLLGYGIGLKYVSPLGPFDLVLGWGDKSVFQPGEIRAVIYF
                     RAGFLF"
     gene            complement(13647..14639)
                     /locus_tag="PLI55_02805"
     CDS             complement(13647..14639)
                     /locus_tag="PLI55_02805"
                     /inference="COORDINATES: protein motif:HMM:NF017832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HPA99798.1"
                     /translation="MDLTQIKTIAAGFYIADENGKKLPGTIKIPYAEKDGFIRHISQV
                     EKGLACGCSCPVCHQPVIARKGAHKRHHFAHYPDAVCNAETVLHYLAKRLLFEKLQTL
                     LKQKVALVLRWKCQLCSDQHSFNFFENVQTVVSEEAFDDCRPDLCLVDQHDAPTALIE
                     IVVAHPPDEKVLIFVSRKQIPLLIFRIRSGEEMEEMATCPTINPDLIIHCPRRRCPEC
                     GAPLFEKSLYILPAVCWRCGARMNLAFLDIDGQVAGPEEFSAKDRQIISRNNVILRDT
                     IRPGDRKRTVSHICPHCGVVTGNAYLRYLKRHEQRLLVQNSGKYCRQCHKHFDE"
     gene            complement(14702..>14923)
                     /locus_tag="PLI55_02810"
     CDS             complement(14702..>14923)
                     /locus_tag="PLI55_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99799.1"
                     /translation="AALQLSRKVMTRIRQNLFWAFAYNAALVPVAAGALYPLFGITFR
                     PELGGLAMALSSVTVISLSLNLRRFNPAR"
     assembly_gap    14924..15023
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<15024..16361)
                     /locus_tag="PLI55_02815"
     CDS             complement(<15024..16361)
                     /locus_tag="PLI55_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPA99800.1"
                     /translation="MFIKISLPFPIAYLMLALATPVFIYLSYPIFRAAGQALRNRSLN
                     MDVMYAMGIGVAFLASLLGTFEIVLTREFMFFETAIFLATFLTLGRYLEARAKGKTTA
                     AIKRLIGLQPQTAIVIRDGVESEIPLESVVIDDIVIVKPGGRVPVDGVVISGESYVDE
                     SMLTGESLPVFKQTGAKVVGGTLNQNGILHIRAERIGRDTVLAQIIRLVEQAQGSRPP
                     VQRLADVAVSYFIPVILAIAILSFGFWYFVAEQTLLFALTAFISVLVIACPCALGLAT
                     PTAVTVGIGRGAELGILIKNGTVLEVAPRVTTVVFDKTGTLTTGKPLVTDIFPVGIAT
                     AELLRFAAAVEKNSEHPLSTAIVNYAKELGIAIPVSAKFAAFGGRGVTAYVDNRVIFS
                     GTELFFQENKITISPDILVPAKKYTGEGKSIVFVAIDGQAVGVIAIADTLKDTA"
     assembly_gap    16416..16515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16614..19466)
                     /locus_tag="PLI55_02820"
     CDS             complement(16614..19466)
                     /locus_tag="PLI55_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99801.1"
                     /translation="MKFIFTTDRIFLRILLIAILSVFILVNSLLAQMLGSDQPPPDIH
                     WRQIETQHYRIIFPAEIESESQRMANTLEYVYLPLRKTLKANSPKWPLVLSNRSAVAN
                     GYVTLAPRKSEWFTTPDQSAMSGNIEWYNLLATHEGRHMVQFDRFNSGFTRLAGFLFG
                     EYAILGLSFWHAPMWFWEGDAVAMETALTESGRGCQPEFDLPIRTLLINQKLYSYDKA
                     YLGSYKDYYPNYYHLGFLMVTHGRRQYGANIWDHVLTSSTRRSWNPFGFSKACRINTG
                     RKTKELYYDTMTELDSLWCKPIEGLEITSAKKVNLKPKKVWTQYRWPQYATDGSIIAV
                     INGLGDINTLVKINPDGQETEIAKIASANRFSTAGNLIAWEELTPHPRWGAAIFSDIV
                     VYDLLVGQERKLTKNGKYFSPAVAPHGQMIAAVKFTPERKCNLVLLDSNDGQEIRTLS
                     NPDNGFIKNPAWSPDGKQLVYFKQSGSSKSLAIYDLENNTEEIIIAECHSFDEYPVFY
                     GDYLLYNSAWSGIDNIYAVEIDSGRRFQVTSRKYGAYYPAVSADGSMMLFSDYDFNGL
                     DVCAMHLEPARWQALEAVERCDIRYYETLVAQEQGGNIFEAGKIPTKSYPVTDYDPYR
                     HLFNFHSWYYFFSETSANLGLFSNDILNTTAANFALNFDVNEKAFSFGSEVCYAGFYP
                     ILSAGFNLGKRISYSGGLFDELTTETWNETSFSLGANLPLDYSSSVFNRKLNLGLSGA
                     YTYISDKTTVEDYEIGNGGFVPFSYEIAYQNYQTPAIRDVKPTAGYTVKANFKHTPLG
                     GDYQSQMYSFQGNYYLPGLAKHHSLMLNAKVELQNPDNYRFASLVPFAKGYDYIYNDR
                     LAGAGATYELPLAYPDWALGSLFYLKRLRGAFFGDFNVGKTSDFKQQYNSVGLDLNFD
                     FNLLQLAVELNAGVRLAYRLAEKDLHASLLIFNIEF"
     assembly_gap    19605..19704
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19705..20774)
                     /locus_tag="PLI55_02825"
     CDS             complement(<19705..20774)
                     /locus_tag="PLI55_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPA99802.1"
                     /translation="MNNILQKSAIFYDVFERKPGTDKLTTTYCPGCGHGNLHKLIAEA
                     LVDFGVQDRTIFISPVGCSVFAYYYFHTGNIQVAHGRAPAAATGLKRAHPEAIVISYQ
                     GDGDLAAIGTAEIIHAANRGEGITVFFVNNAIYGMTGGQMAPTTLIGQKTMTTPYGRN
                     ANNEGYPIGMSEIIATLKAPVYVERVMIGDTKSILHARQAVRKALKLQIEKNAFCFIE
                     VLSPCPSNLKMSAIRSREWVCEVLQKQFPLGVKKDISNEFPSRDLKRPTIDRSQLHSI
                     LNIEPITVDKEKIAPYKKTNFSAEIKVAGFGGQGILSLGLVLAEMGMRHEYEVSWLPS
                     YGPEIRGGTANCSVKISNRKIGS"
     gene            complement(20771..21823)
                     /locus_tag="PLI55_02830"
     CDS             complement(20771..21823)
                     /locus_tag="PLI55_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPA99803.1"
                     /translation="MAKQFLKGNEAVVKGAVLAGCQAYYGYPITPASEIAHAAALYLP
                     VSGGTFLQAESEIAAINMVYGAAAAGQRVMTASSGPGISLKQEGISYCAGSELPCVIV
                     DVMRGGPGLGNIAPEQGDYNQVVKGGGHGNYKCIVLAPNSVQEMCDFGMLAFDLADKY
                     RNPVYILSDGFVGQMMEPVEFPEPVKQFPEKSWAIKGTPETSDNLITSIELDPDDLER
                     HNLKLQQKYAIIEEQEVRFEDYSLSDAEIVLVGFGIISRILQTIVDNLRDVGVKVGLL
                     RPITLFPFPKKKISQLAEQAKFFLVVEMSNGQLVDDVRLAVAGRKPVYLYNRLGGNVP
                     SEKEINDELLKLMRSV"
     gene            complement(21985..22224)
                     /locus_tag="PLI55_02835"
                     /pseudo
     CDS             complement(21985..22224)
                     /locus_tag="PLI55_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
     gene            complement(22243..23427)
                     /locus_tag="PLI55_02840"
     CDS             complement(22243..23427)
                     /locus_tag="PLI55_02840"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPA99804.1"
                     /translation="MPISNYIAQSMTRSSWIRKMFETGIALKKQYGEDKIYDFSLGNP
                     DLPTPEIFNRVLVEESARTGAYVHGYMQNKGYPETCAAVANLLERESGLPFTEDQVVM
                     TCGAAGAINIILKSLLDPGDEVIVIAPFFSEYEFYVDNHRGQLVISEANAELLPDPAD
                     IAAKITPRTRVLLLNSPNNPTGRVYGEDIFREIGKLLTEKSAEFGRPIYLLMDEPYKR
                     IIFDGLQYRSPFRYYRHTILASSFSKDLSLAGERIGYLAISPLIEDYPALINAAAFCN
                     RVLGFINAPALMQRVIRRVLDAQVAIELYQKRRDMIYDALVKIGYEIIKPEGTFYLFP
                     KCPIDDDVKFVFALQDRLIITTPGIAFYRPGYFRISYAVPEAVIQAAIPIFKQVFNEM
                     KP"
     gene            complement(23435..24898)
                     /gene="gltA"
                     /locus_tag="PLI55_02845"
     CDS             complement(23435..24898)
                     /gene="gltA"
                     /locus_tag="PLI55_02845"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011358419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPA99805.1"
                     /translation="MSSSNLSKKERLAIPRQQMPEQPPEERIHNFNEVNLGFDAETAI
                     REAQRCLECPRPTCIDGCPVSIKIPDFIRLIVQRDFSGAARKIKEDNLLPAVCGRVCL
                     QEEQCEKTCVIRGKFGPVSIGRLERFVADYERQNGQPQVSLSTPRNGKKVAIVGSGPA
                     GLACASDLIKWGYDVTVFEALHEFGGVLIYGIPEFRLPKAILKYEIDNLRKLGVRFEA
                     NFIVGKTMTIDELFQVAGFDAVFLGVGAGLPWFMNIPGENLMGVYSANEFLTRVNLMR
                     AYEFPEADTPIKIGKQVVVVGGGNTAMDAVRTARRLGAEKATIVYRRSEVEMPARLEE
                     IKHAKEEGVIFRTLTNPIEFLPDENGWVRAARCLQMELGEPDSDGRRRPIPIPGSEFE
                     IPTDLVIMAIGNGSNPLISRTTPDLQCNKWGNIITSGEGGRTSKRGVFAGGDIVTGGA
                     TVILAMGAGRAAAHSIDEYLKTGIWVTEPDSANKNKT"
     gene            complement(24919..>25305)
                     /locus_tag="PLI55_02850"
     CDS             complement(24919..>25305)
                     /locus_tag="PLI55_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99806.1"
                     /translation="GLVTDVLKTIIAGKPQPNFVLAVGPLPMMRAVSEVTRPHAIKTM
                     VSLNSIMIDGTGMCGGCRASVGGKTVFVCVDGPEFDGHQVNFEELAHRQKQFAAQEKI
                     SADILNTEPDKVLHQCRLSKVISENS"
     assembly_gap    25306..25405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<25406..25647)
                     /locus_tag="PLI55_02855"
     CDS             complement(<25406..25647)
                     /locus_tag="PLI55_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPA99807.1"
                     /translation="MTNDNFKWEDLDKDGLTYLMENRKEVLRNYQKMINSLGNHLDEK
                     TRHLIMLAIQINSPSPEAIKIILPKAIRAGATRDEI"
     gene            complement(25665..26693)
                     /locus_tag="PLI55_02860"
     CDS             complement(25665..26693)
                     /locus_tag="PLI55_02860"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPA99808.1"
                     /translation="MDPKTIEWSKLEFKYSYTPFRFQARWRDGHWDDGQLVTDKKISI
                     EEGSTCLHYGQEVFEGMKAQRTRDGRVMLFRPRDNAARLRRSAARLLMAEVPEDLFMK
                     GIIETVKANLDYVPPYGTGASLYIRPFQIGVGDNLGVKPAPEYLFSVFVCPVGPYFKS
                     GFQTIKVKVEEYYDRVARHGVGQAKAGGNYSASLLPLKIAHDEGYNELVYLDPVEHKY
                     FEEISSSNIFFVFKDGSLATPKSESILESITRLSLVRVAREEFGLNMEERRISIDEIE
                     NFAEAGACGTAAVITPIGSLGHRGKLYKFYADGEEAGPITTKLYKYLTAYQVGDVPDR
                     FGWLEEIK"
     assembly_gap    27137..27236
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27237..27986
                     /locus_tag="PLI55_02865"
     CDS             <27237..27986
                     /locus_tag="PLI55_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPA99809.1"
                     /translation="PTLPEVVDQYLSTGKYDVFTITHNETSTGLMNPVAEIAEVKRKY
                     PDVYWLVDAVSSMGGIKIPVDELGIDVCITSTQKCIGLPPGMAICSVSQRALEHARTV
                     PFRGAYLDLVELYDYLIKKDFQYPSTPSLSHMFALDLQLDYILNVEGLEKRYARHREM
                     AEITRAWAKENFAVFPQPGYESVTLTTITNTRGISIADLNKRLGEAGYQISNGYGELK
                     EKTFRIAHMAERTVDELRTLLNLIDTITAKM"
     gene            28010..28909
                     /locus_tag="PLI55_02870"
     CDS             28010..28909
                     /locus_tag="PLI55_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HPA99810.1"
                     /translation="MKILIADGLDQKAQNILKEAGLELVVKHYEADDLIKVIQEFDGI
                     IVRSATKVPQVVIDAGKNLKFIARAGTGIDNIDHQYARAKGIAVLNAPGANSHSVAEL
                     VFAHLLALARFITPATISLREGRWEKKTFGNGIELAGKTIGIIGFGRIGKIVAHLAIA
                     WGMHVLVYDVATINTEPTVKVVDFETLLKESDFITLHTPKTDQPIINEKEIAMTKRGV
                     YIINCARGNAIDEKALLAGLNSGQVGGAGLDVFAAEPPQNMELLHHARVSCTPHIGAS
                     TLEAQERVGAEIAHRIVEYIGTL"
     gene            28918..>29286
                     /locus_tag="PLI55_02875"
     CDS             28918..>29286
                     /locus_tag="PLI55_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019908151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HPA99811.1"
                     /translation="MVKIKPFRGIRPVKELADKIAAPPYDVINFEEARSLAADNPYSF
                     LHVSRAEIDLPPETDPYDEKVYQKARENLEAMLAKGWLFQDATEMLYIYRQIMDKHSQ
                     YGLITTANVNDYLKGKIKIHE"
BASE COUNT         8095 a         6567 c         6292 g         7732 t
ORIGIN      
        1 ccccgctgat agttgccgat gttaccgaaa catcaatgtc cttgccgttc acaaagccaa
       61 ccgtcgcaac gccataatca tcagtgaaat tcttctgaat atatgctgct gtgggaacac
      121 ttctcttaaa cccgcctatt acagtaggat ctaccgctag gtgagcattg agtcgtcctt
      181 cggaattcca taaacctcta agattggtca ccggatcagc agtagccagc gtgcgagcga
      241 taagatcatg agcatcatct tccgggaaaa cgctctttgc tagagcgatt acgccagtga
      301 catgcggcgc cgccatagat gtgccgctca tatacacata actattataa ggataacaag
      361 acgcaatatc agtacctggt gcgccgatgt caactgtggc ttgaccgtaa ttcgaaaaat
      421 ccgatttatt gtcagcataa tcagtggaac cgacactgat aacattcggc gattggatta
      481 agcgcgggta agcataatat tccagcttat ctgtattgac gtcgtcattt ccggctgagc
      541 aaacccagat cataccataa gaatcgtgtt cccgggcata cttggtcata acgtcaacaa
      601 aacgctgttc atcgcgatca ttcagatagg cgccaccgcc ccaggactgg ttgacggcaa
      661 caatttttac cccagatttc agtaaagttg acagataata atagccatac atcagggtaa
      721 tcgcgtccgt agaaccataa tcgtcccaga tcaaaaccga cagcatctta atgtggtgat
      781 taacgccggc gatacccagt tcgttattac cttcagcgcc aatgattccg gctacatggg
      841 taccgtgata actaccgctg ttcaggtagg aattgtcatc gttcatcaca ttccaacccc
      901 agaagtcatc aacgaaacca ttgccgtcat cgtcaattcc attccattct ccatcatcca
      961 cgatgccgtt gccgtttagg tcttcaccag gattcaccca gatattgtct tgcagatctt
     1021 catgatagat gtcaataccg tcatcataaa ctgctacaac aacatcagag gtaacttctc
     1081 cgatggtata ggttccgcca gtagtgatgt cccaggcgtc aaaagcgcta atatcggcgc
     1141 ctgctacagc atccacaact ggcatacctc ccatactggt atataacgaa tcaaatgttc
     1201 catcgttgtt cagcgcccat tgcagggaaa tgaagtaatc gttcgggaca agagcatctt
     1261 tttgaatagg tttaggttgc aaattctccc gtttaaaaac aaaattcgga aaagcgctga
     1321 tgccgtcaat ggcattcaag gcatcgagag ttttccgcat atcgccttta atctcccact
     1381 cggcaattcc ttccgtgggc aggtttcgga cgatacgtcc ctgataagct tggcgggcgc
     1441 tttcgagccg catggtctga tcaggtttgg cgggcaattt tttcaaaatt gaattattga
     1501 ccaccacgat tcggtttgag cgaatttctt tgccgttata agtcgcaatg ttaccggcgg
     1561 ctgtcagaga aacagtcacc attaaaaacg cgattaaaat gcagagaaaa gctctcgtgt
     1621 gagatgtttt catgttatgt cctccatttt aaattaataa ccaactcaaa ataccttttg
     1681 tataagctta ggggaaaaat aacaataatc aaatggtatt ttatggtatt ttaaaaaatg
     1741 tgatttaccg catatctgta attgtagtat gccatacttg acttatttat taatttttca
     1801 gtaaagttaa agtatgtcga atattcagga aattaaggtg cgcagtattc tcagccaatc
     1861 caaattgcca ggggtggatt tcgtcatcaa tccctatcgc ggatgtcagc atgcttgcgt
     1921 ctattgttat gcctgtttta tgcagaagtt cgacgggcac agcgagccgt ggggaactta
     1981 cgttgacgta aaaagcaatg cgctggagat attgactagg caattggcga aatgtcacaa
     2041 ccggaccgtt tatctgtcct cagtctgcga tccttatcaa cccgtcgaag ccgaatatca
     2101 attaacccgc caaatcctaa agcacttgat aatagctcaa ccccaactaa cagttcagac
     2161 caaatcggct ctggttatac gagaccttga tttattggaa aaattcaaaa attgttcagt
     2221 aggaatgacg atcgtgacga ctgatgaaaa tttacgtcgt gagattgagc ccggcgcatc
     2281 gcccatcgcc gctcgtattg atgccttaaa acagctcaaa gccgccggat tagaaaccta
     2341 tgttttcgtt ggaccgattt taccttatct gaccggctgg aaacagattg ttcttcagac
     2401 ccgcgaattt attgattatt atatgttcga ccataccaat ccatctgggc gaatctggag
     2461 tcatataaaa agctggttaa ttgccaaaca tcccgaactg ctaagcagtt acgaaaaaat
     2521 cttgttcggt cgcagtgatt actggtggcg agtcagtcgg gcaattcaag acttttgcga
     2581 caaggaaaat atcaatcatc aaatattttt ctaaagtaga aaaccttacc agaaattgaa
     2641 aaccaaactc aaaaatctag cgacccattg aatcagcgtc aactgttcta aaaaagatac
     2701 tatgaactgc taaatagttc tgaactcaaa tttttcaaag tggcgaatta tttccctggt
     2761 aatgcaccaa tttccggcaa ttctaatgtc cgcagtaaaa ggttgattct ctctacgttt
     2821 tttcgcataa tcctttggat gaactccgcc aattcggcgc cattttctaa ttccagacca
     2881 tgggcaaggc aaaacggtcg aaccagctga tccaagtaag ccttatccag gtgatcatcg
     2941 ttagaaactg acaggcgata tagatcgcga atatgcttca gcaactgata atcgcgcacc
     3001 agagctatga agttactgtc gtcattggtt attgccgatt caatccgcga aaacaatgtt
     3061 tcggtcaaag gttcagccca ttcgaaatag gcttttagaa taaagataaa attctcgaaa
     3121 tcgcgcaagc cgcctggacc ttccttgatt tccggaactt caattttagt actattataa
     3181 taattgtggc gggatctcat ttcattcaac atcgcttcga taaattcagc cttacgctgg
     3241 aaaatgaatg gagtaataat ttcactctga aatactttca aaaaataatc acttccaatt
     3301 accagacgcg cacccagcaa ttgcgattga tcaatgaaag tattttcatc gggtcgctcg
     3361 aataatctac gcagatcgct gaaagtggtc acaaatcgcc ggaaacggtc agcaaagcgg
     3421 aattggggaa tgatggaacg tcgcaggatg tatttattca ttttttgtat gactttatta
     3481 ccgaagtcta aaatagccgg attatcagaa ttcaataaaa caatcaggtc ataatcatcg
     3541 tcaaaagcct gaccgcgggc atgcccgcct gccgtgaaaa tagccaaaag atcatgcatt
     3601 tccgggatct ctttctgatc agccgccgct acttcctcct gacagagttc aaaaagcatc
     3661 tgaagataat tatccgaaaa attcgtgaat tcccgattaa ttttatcgaa gggcgcgccg
     3721 tctaataaag caattcccaa acgcataaac tcgaaatcgt aataatcacc caaccgggta
     3781 attttttcat tgagcgaatc gaaattatca atattgcgga aaagaccttc agccaattga
     3841 ttgagcttag gttgattggt taacgaagtg atgtattcag cataagtatt gaacgcccgc
     3901 tgtacaaaac gcttgaaata ataactacta ctgcgataaa tccgacaaag cattaatagt
     3961 gtatcgcgca gagaggtaat ttctggtttc cagtaaggtt cttcgacaac tgctatcaac
     4021 cgcgtcaaga gctcgaccgg taaaattgac aggaagttat tcatcatctg cggagctaaa
     4081 ttcagaattt gacaaagtcg gctgacattt tccagagtcg gttccagatt ataaataaaa
     4141 tcgcgaatga attcctggaa aaactgcgtt tcgactattt tcggttgatg gtcagcgata
     4201 atgctgaaca gcgaaatcat agtataagga gtttttacac cccatttgac ataagctttc
     4261 atcaggacag cccgccttgc ttccgataat ttcataaaat ctgccagaaa gcgtttcagc
     4321 aaaatttggt tgggattatc gagactggag agtaaatcat cccagaaacg tacaccttga
     4381 aaaaagcggg cggtctgcat aaaattaagt ggaatattat ccggcagagg cgctttgctt
     4441 tcggcgcggg attggaaatg cgtgatcggg tagaagatcg taatctggcg aagatgttcg
     4501 gtcagccctt ctactagacg ctcagttcct aaccgggcga ttttatgatg atcataatag
     4561 tgagtcaaca tttgagtaca ggctaaagcg taactatgat cttggtaacc catcgcatag
     4621 gctacttttt gcaggctaag ttgcatttca tcgctgtcta aatgaatttc ttcctcctgt
     4681 acgacgaaca attgatacag aaagcggaaa gtttccagaa aagttagtgc ctgaaaaacc
     4741 tgataataat tttcatgatt gaaccgttct ttgactaata aaatgtccaa aacttccagc
     4801 gaggtgttgc cctcaacccg cttccaagtc ttcagtgcaa aaattagagc tttcaacatc
     4861 cgcaagccgt cgcgtttggg attgagtaaa ttttccggcg gctctttata catcaagtcc
     4921 cgaatttcac ccaataagcc gcgcaaaaaa ccttcgtggt atttattatc tcggtcagcc
     4981 gaaaaataat agcgatcgag aatttcattc ttaaatcggt caaataattc acgattacca
     5041 agaattggaa cagcgttgac catttcacta atcagcacaa aatcctgaat ttggtggtcg
     5101 agtagattgt ggtaatcctc aattgaagcc gtatagcctt tggtgccaac atgctcggaa
     5161 agatgaaagt gaagtgaaca ggcatatttg agcatttcgt tactcatcgc tccgaaagca
     5221 ttggttaaaa tcgctcgatt tgccggtcca gtatcaacaa ttcccagatc aatgtcatcc
     5281 tggtcaactc gcgtaccaac gccacaaatg acgaaatcag gcctttgagc gcgcggcagg
     5341 aaaatatcga gcaaggtgtt catatatgcc gcggtcagca aacggaaatc gttaccggtg
     5401 cgtaagagaa acgaccgata aacggcatag tgattagtag tagtacttag tttcatcttc
     5461 aagacgtcaa ttgccttgaa attcaataat aataattgga tagaaaagta gcaacccagc
     5521 atttccagta aggtttctcg attcgctact aaatcggcga tcagcttcat ttcgcgggcg
     5581 gggtgtggcg aaaaacgtaa cagatgaaat tggtcagtta ggtgttcgtt attaattaat
     5641 gcaaacaaat tatcgaaatt catctcaata ttgtggataa atttccggtg atgattcccc
     5701 agatcggaag cccgattgag aaggtctgtc agagaataat gaatcgctga tttcatcgtt
     5761 cgggacgaaa attgaatggc ttaacgagca tgttctttaa gccattcttc agccagctcg
     5821 atgccggttt gaaaggcttt caaattcaat tcctcagtgc ctttcggaac gcgggcgcgg
     5881 atagcatttt tgatagcgtc ttcggaaaca atacccgcta atttataaat cacaccgaga
     5941 gcgatgatat tggaaaccac aaatttaccg aggcgctcga cggccgattg aattatgggc
     6001 aaagccacaa ctttaaagtt gccatcggga attttggtta ccatttgcga atctacgata
     6061 agaattccat ccggtttcag atcggcaata tatttatcgc aggcttcctg ggtcaatgcc
     6121 agtaacaggt caatattctc ggctttcgga taatcaatat cgccgtctga gatgataact
     6181 tcactgcggc tggctccacc gcgtgcttcc ggaccatagg attgactttg agtagcattc
     6241 aaaccgtcat agatcgcggc ggcttcggct aagacttttc ccaccaaaat cagcccctga
     6301 ccgccagcac cactcaacct tatttcataa tgaaatgaca taattttgct ccttatttaa
     6361 tttctgatcg ggctttagtc acaactgcct cgtaatgcga ttgatagtca ggctgttgac
     6421 gatcaacgaa aactcccaca accagtttat tccgtaattc ttctgggctc atttcgctgg
     6481 cttttgcgag tgacacggta ttttcttttt gataaagcaa catagcggcg ccattgcctt
     6541 ctttattcat tcgtccgtaa gaagtcgggc agggcgttaa aacttccacg acggcaaagc
     6601 ctttccgggt aagggcttgc tcgatgtatt tatcgaggcg agcgtaatgg aaaaccgttg
     6661 aacgagccac ataacttgcg ccagccgccg ccgccaaacg actgatatca aaaggtggat
     6721 cgggattgcc gtaaggggcg gttgttgcca ttttaccggc aggtgtcgtc ggcgaaacct
     6781 gcccgccagt catcccgtaa atcatattat tgaaaattat catggtgata tcaatattgc
     6841 gacgggcggc atgaataaaa tgatttccgc cgatcgcagt cgcatcgccg tcgccggaaa
     6901 ccacgattac gtgtaatttt ggattagcga ttttcaagcc ggtggcaaaa gccaacgccc
     6961 gaccatgagt cgtatgtaaa gtattgaaat caacataacc gggcgttcgg ctggaacaac
     7021 cgatgcccga taccagcgca atgtcattct tatcccaacc gagtttattg attacccgca
     7081 ggagcgattt cagcacaatg ccgttaccac agccgtcgca ccaaatgtgg ggcaaatttt
     7141 ttgtgcgcaa ataatataaa tagtccatac ttccctctaa tttttaatcg ccgccagaat
     7201 ttccgctggt gtaattggtt cgccgttgat gcgattgacc cggatgactg gtttagcgcg
     7261 gcgaactgtc cattcgactt cgtgagcaat ttgacccaga ttcagttccg gtacaatgat
     7321 tttattcacg gatttggcga gcgcttcgat ctctatatca gcgaaaggcc agagcgtttt
     7381 tagctttaat agaccggctt taaggccagc ttcgcgagcg aggctgacgg cttttttagc
     7441 cgagcgggca gtcgctccat aggcaatgac aaggatctcc atatcttgcg tctcaaactc
     7501 ctcaacctcg acgatttgat cgcgataacg ttcgattttg cggttaatgc gtagaagctg
     7561 ttgttcgatc tgggctggat cattggttgg tgagccggtt tcgtcnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnngccat aattgcaaac ccgatatttt cctgctttaa
     7741 agaaaatccc gggccgctgg tagcggtcat agattttaca ccagccagag aagccccaat
     7801 cacagccccc atcgcggcga tttcgtcttc catctggata aaattgcccc caatttgcgg
     7861 cagacggcgc gccagcacct ccgcaatttc ggtagaaggc gtgatcggat atccggcaaa
     7921 aaaacgacag ccagcggcga tggcgccttc cgcacaagcg gtgttgccct gcatcataaa
     7981 aattcgagaa ttcgagctac ttgcattgga tttattcatt ttgcctcctc ctttgctttt
     8041 tcggggacgg tgacgacgat ggcaaaatcc ggacaaagac tttcgcaaat cttacacccg
     8101 ttgcaatttt ccggatggac aacctgggct gtaaaccgac ccatttcgag tacattgcgg
     8161 gggcaaaatt tgacgcaaat ttcacaggat ttgcaccact ccggatttat ttggacttca
     8221 ggtttggtca aggcaattct ccttttttac ctattgagaa aaacaacctc ataaacttaa
     8281 taaattaagt cgaattgtta cccagaaatc ttgagactgg cggtgctttg ataggcaatt
     8341 cggttgactg aatcaatttg cttcataaac cgagaagtgt tagacgcgga ggattggtgc
     8401 tgattcgggc tcggattcca atcggaattg taaaacgctg tttgagatgt ccgtaggcac
     8461 aattggtaat gaccgggaaa tgggtatctt tcaaggcgtt actaagataa tcctccaatg
     8521 taaacgaacg tttaggattc gacggaaaac aattgacaaa ctgcccaaca atcaatccgg
     8581 caatctgatc aaaaacgccg tgcaagcgta aaatttgcag ttgcttgtca agccgatagg
     8641 ttttttcgtc aacatcttcg atgatcagaa tcgcgccttt taaatccggc ataaatgccg
     8701 ttcccagcag aggcgttatt aaagaaagac atccgccgat caaaacccct tcggcggaac
     8761 caggttgaaa cgcctgcaat ggctgagcca atggattagt cagttcgatc ggataaggat
     8821 tactggtaat catacgccag agccattcgg cgctaaaaga actgagactt tgtcccaaat
     8881 cagtagaaac cattggcccg ctatacgtca cccaaccgag tttagccgcc agagccattt
     8941 gcaaaatggt aatgtcggaa taaccaacca atacttttgg tttgattttc gctaaacgct
     9001 gataattgag ttgatcaatt atccgaatta atccgtagcc gccacgcacg caaaagagag
     9061 catctatttc cggatcgcag agaaattgct cgagatattt taatcgctcg gcatccgaac
     9121 cggcgaggaa gaattttccc cgatttagat tcgatgcgct cttaacttta taacctagat
     9181 tttggagata ctgtaaaccg agcgccgtag tttggcgggg cgcggggctg gaaggcgcta
     9241 taacgccgat tgtccctccg gtttttagcg gttcaggaat taagtatggc ataaatctct
     9301 tccactattt cttcataaag ttgcatatct aaaatgaaaa gcaaaaatgt tttttgattt
     9361 ttaaatgggt agagataagt attgatgtag taaatttttt cagcattgga ttttttaacg
     9421 aaattattct aatttagtgc aagtaaattt gagatttata tcaacgtgag gataaaatga
     9481 aattatcaca gaaatctatt atcaatctgc tgacatttgc ttgtctttta cttctaaatg
     9541 aatccggctt gacccaggac aaagagttga ttgataaagt tagtgggcga atggaatatg
     9601 attttcaaca gatgactgag gaaagtgcgg taggcgacgt taacacgctt cggcagttta
     9661 ttgctgattc actcaagttt gtctatggag aaaggtacca gcaagaactt gactctctgg
     9721 aatcaatcta tatgaccacg attaaaactc tggagactga atgcactcgc ctgaataatt
     9781 tgaataaaat cttaatcgac agccttgaga aagcccgatc tgccagaccg aaatctgtca
     9841 tcgaaaccaa agacctcgat cccacttttg aatcccgata ctttcagtac ggaaaaattc
     9901 tcaggctgga agagcccaga cataaaactg gttttttaaa aatgccgacc gtgatcgaaa
     9961 acatagcacc ttttcaaatc caagaattga atgaatatta cgatagatat tttcctattg
    10021 ctcggagtga ttcagttctc gattttatca tccaaatcaa tattcgcgaa aaagattggg
    10081 gtaatgccgg atgcaatatt atcaagtttc tttatctttt ccccaattca gcgctcggtg
    10141 aggaaattaa aaccgttcgg gctggcatct ttcggacaga gaaatattac caaccttata
    10201 gcgctttttt aaccgacctg cttaataaga ttccccaatt accgctcgca gaccagcgct
    10261 attatcatta tgtggaatta ctcaaaggct ttcctgatca ggctatccga aacaacttca
    10321 taccggaagc ctataaattt ctggagctat atccaaattc caaatacgcg gcgcaagcca
    10381 atttgtggat agccgaagct cttttagcta acaaccagcc gcacagtgcc tatctgacat
    10441 tacagaaaat aatgatactt tacccaagtg atgaattata ttgccgcgct ctgcatacca
    10501 gcggagttat tcagcaggaa cagttcaacg aatataaaaa cgccgttcaa acttttactg
    10561 atttaattaa acgctttccg caagataccc tggctgagaa tgcccattat cgtatcgcta
    10621 aaatcagcga cgagtattta aatgattggg aacgagccgt cgaacaatat cagatttatg
    10681 ccgataaata tccgcaatcc ccgaacgcta ttccggcttt aatgcggaaa gccaagattc
    10741 aatctaccca gatgaattta attgaagaag ccgtgcggac ctataaatcc attgatgaac
    10801 gctatccttc cacgaccggc gcattcaatg ctctgacgga ggcgggcaat ctttatatga
    10861 ccaagggacg ctacgatcag gctattgaaa tttatatgag tatttttcag aaatacccgc
    10921 aatcggaaaa cgccgtcgcg gctctcgaaa aagtttacaa tatttatcaa acgaaaatca
    10981 aagataacga gaaaagcatc gaaatattaa acttaattat aaacaattat ccggatacca
    11041 aagcttccgc cagagcgact aagcaattga ggaagctcga gaaagcccga tagttcccta
    11101 tagactgcgt gtgaattttc cgatggcaag ttgctctaac ctcaatttta gtagataccg
    11161 atttatgata aatggaatta tatccattat tgttctatct ctaactacca ccagtttacc
    11221 ggctcaaacg ataactcgtc ccaaaatcgg actggctctg agcggtggcg gcgcgatggg
    11281 ttttggacat ataggcaccc taaaattgct cgattcattg agcattccgg ttgattatat
    11341 cgccggaacc agtatgggag gaattatcgc cgccttatac gccgtcggtt atactggtgc
    11401 agaaatcgaa gaaatcgctc gaaatgttga ttgggcggat ttgttcaatg atgcaccgtc
    11461 ccgacaggct ttaccttatt ttcagaagca ggaagccgat aagtatcaat ttgagttagg
    11521 tattcgcgat ttgcggccgg tagataaagg gggcgtgatc gctggccaaa aaattacgct
    11581 ctatatgacc cgcttagttc tgccattttt aaccgttagc gactttgata gtttgattat
    11641 cccatatcgg tgtgttgcag ttgatttgac cactggatcg gaagtggtta tagatcatgg
    11701 ttctctgccg attgcgatgc gggcgacgat ggcgattccc agtattttta ccccggttgt
    11761 gtggggcgat tcgcttctgg ttgatggcgg attgctcaat aatctgccgg tcaatactgt
    11821 cagaaaaatg ggcgctgaca tcgtaattgc ttcaaccgtc aggagcccct ttaaaaccag
    11881 ggatgaacta cggaccacct tggatatctt gtcccaaaca tccaatattt tgcgtgataa
    11941 taagcttgaa gccgaagcta aaaatgccga tttactaatc aactgtcagt tagaaaagtt
    12001 aggagcggct gatttttcga ccgctaaggt atgcaaaatt attgaggcag gcgaagccgc
    12061 agcgcatgct aatctcgatg aattattaga tttgaagaaa aaatataact tgtcaagata
    12121 tggtcctgag aatattaatg atttgaattc tccaagtggt taccgggttg ctcgactcat
    12181 catcaaaggt agtcaaacca ttccggttga cgtcgttcgt tcgactattg gtattaaagt
    12241 tggcgaatta tattctccgg aaactctagc ggcgggtgtt gagcgtttga aatcttttga
    12301 taattacatt cgtgtcagac cgacggtcga aatactctcc gattctacgg ttgaaatcaa
    12361 aattaattta gtcgagaagg tcaaagcaca gatcaataat atcgaagtac gcggcaatga
    12421 atatttaacg gatgatttta ttcggcgtag ttttggaatt atccctggtg aggttttttc
    12481 gatggaacgt atcgagtcgc agattaatta tctatatggt ctggattatt ttaataatat
    12541 cagctatact acggaaccag ccggggaagg taagatcaat ctaatactga atgtcgtcga
    12601 gcatacacct cagaaactgc gcctcggggt gcgatatgat gacttccatt atttggtagc
    12661 ggcgctgaat ttccagacaa cttcaaatat agttcatgga ttaaggctcg atgtcgaatg
    12721 gcagttcatt ggactgaatc agattaagct caaaacgctt tatccctcgc ggcgcttcca
    12781 actgcctttt tatccatttg tcaacgccca ttatcgcgaa attccaacct atgtctatag
    12841 cattaacggc aatagaatcg catcatatat tgaccgttca gcgctcttcg gtggcggttt
    12901 cggtttgctt tataaaaatt actggaattt cgaagcggag ctggattacg aatcaattaa
    12961 tatcaaaccg gatatcgcac cggatgaacc gttgcgattt tatgattgga atgatgacct
    13021 atataaattt cagttgtctt ctaacattga tcttctggat aatgcattta tgccgcgcaa
    13081 aggcgctttg gttcattgcg tttacgaaaa gacaatctcc caaattggtc ggcataccaa
    13141 ctatcggcgt tttgaacttt cagccgacgt atatcagtcc atcaaacgca acactttgca
    13201 tttttcaggt ttttacggga atgctaagat ggacggttct accaaccgtt ttatttatcg
    13261 cggcgggcaa gaaacttttg taggagtcga atatgatcaa ctggttggtt ctgaaatgac
    13321 tattctgcgt ttggattatg cctacaacct attgcataat ttgcaagcta aagcaattgt
    13381 caatacggcg attggcttcc gcaatattta ccacgaaacc ataaaagagt ctcagaatct
    13441 attgggttac ggtatcggtc tgaagtatgt ctcgcccctt ggaccattcg atttagtgct
    13501 gggttggggc gacaagagtg ttttccagcc tggagaaatc agggcggtga tctattttag
    13561 agccggattc ttattctgat agggaactgg atttcagttt ctcgaatctc gtaaaaaaat
    13621 aatcgccaaa tagctcagat agaaaactat tcgtcgaaat gcttgtgaca ttggcggcaa
    13681 tatttgccgg aattttggac tagaagcctt tgctcgtgac gttttaggta acgcaaataa
    13741 gcgttgccgg tgactactcc gcaatgggga cagatatgac taacggtgcg tttacggtca
    13801 ccaggacgga tagtgtctcg caggataacg ttatttcggc tgattatctg gcgatccttg
    13861 gctgaaaatt cctccggtcc ggcaacctgt ccgtcaatat caagaaaagc taaattcatc
    13921 ctagctccac aacgccaaca aaccgctggc agaatgtaga gcgatttctc aaaaagcgga
    13981 gcaccgcatt ccggacagcg cctacgtgga caatggatga tcaaatccgg attaatcgtt
    14041 gggcaagtag ccatttcttc catttcttca ccggagcgaa ttcggaaaat caatagcgga
    14101 atttgtttcc ggctaacgaa aattaaaact ttttcgtccg gtggatgcgc cacgacgatt
    14161 tcgatcaaag ccgtcggtgc atcgtgctgg tcaaccagac aaagatccgg gcggcagtca
    14221 tcaaatgctt cttcactaac taccgtctgc acattttcaa agaaattgaa ggaatgctga
    14281 tcactacaaa gttggcattt ccagcgcaaa acgagagcca ctttttgttt caataaagtc
    14341 tgaagttttt cgaacaatag gcgcttggca aggtaatgca gaacagtttc ggcattacaa
    14401 actgcgtcgg ggtaatgggc aaagtgatgt cgtttatgtg cccctttgcg cgcgataacc
    14461 ggctgatggc aaaccggaca ggaacagccg caagccagtc ctttttcgac ctgggagata
    14521 tgtcttataa aaccatcttt ttccgcatag ggaattttta tggttccggg aagtttttta
    14581 ccgttctcat ccgctatata aaatccagcg gcaatggttt ttatctgtgt gagatccatc
    14641 tatcgcaacc agttattttc attactaaat attaaaataa tagcgaatta aatgaaagag
    14701 tttatcgagc cggattaaaa cgccgcaggt ttagtgaaag actgatgacg gtcacggaac
    14761 taagtgccat agctagtcca ccaagttctg gtcgaaaagt aataccaaac agcggataaa
    14821 gcgcccccgc cgcaaccggc actaaagccg cattataggc aaaagcccag aaaagattct
    14881 ggcgaatgcg agtcataact ttgcgactga gttgcagagc cgcnnnnnnn nnnnnnnnnn
    14941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnggcggta tctttcaatg tatcggcaat agcgataacg
    15061 ccgactgctt gcccgtcaat tgctacaaaa actattgatt ttccctcgcc ggtgtatttc
    15121 ttagcaggca ctaaaatatc cggtgaaatt gtgattttat tttcctgaaa aaataattcg
    15181 gtgccgctga aaattacgcg attatcaaca taagctgtaa cgccgcgacc gccgaaagcc
    15241 gcaaattttg ccgatactgg aattgcaatt cccagttcct tggcataatt cacaatcgcc
    15301 gtgctgagcg gatgctcgga atttttctcg accgcggccg cgaagcgcag taattctgcg
    15361 gtagcaatcc cgacgggaaa aatgtcagtc accaacggtt tgccggttgt cagagtacca
    15421 gttttatcga aaacgactgt ggtgactcgc ggtgctactt ctagaacagt tccattttta
    15481 attaaaatac caagttcggc gccacgcccg atgccgactg ttacggcggt gggagtcgct
    15541 agtccgagtg cgcagggaca agcaattacc agcactgaaa taaacgctgt aagcgcgaaa
    15601 agcaatgtct gctcagcaac aaaataccag aacccaaaag acaatatggc aatggctaaa
    15661 atcacaggaa tgaagtaact gaccgccaca tccgccagtc gttgaaccgg cgggcgactg
    15721 ccctgagctt gttcaaccag acgaataatt tgcgccagaa ccgtatcacg cccgattcgt
    15781 tcggctcgaa tgtgtagaat gccgttctga ttcagcgttc caccaacgac cttggcgccg
    15841 gtctgcttga aaaccggcag ggattcaccg gtcaacatag attcatcaac atagctttca
    15901 ccgctaatta caactccatc cacgggtacg cgaccgccgg gcttgacaat cacgatgtcg
    15961 tcaattacaa ccgattccag cggaatttcg ctttcgacac catcacgtat aacgatggcg
    16021 gtctggggtt gaagcccgat caagcgtttg atcgcggcgg tcgttttacc tttggcgcgg
    16081 gcttccaggt agcgcccgag cgttagaaat gtcgccagga aaatagccgt ttcaaaaaac
    16141 ataaattcgc gtgtcaaaac aatttcgaaa gttcccagta ggctggctag aaaagcgacc
    16201 ccgataccca tcgcatacat cacatccata ttcaaactgc ggttgcgaag cgcctgccct
    16261 gccgcacgga aaatcggata actcaggtag atgaagaccg gagtagctag cgccagcatc
    16321 aaataggcaa ttggaaacgg caggctaatt ttgatgaaca ttggaatcat taaaattacg
    16381 ccggtaatga aaccaataat tatacgtcgc ttcctnnnnn nnnnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnataac ctgatttata ttgtcgtttg gtttcataat aaattaactc
    16561 tcagttttaa tcatcaaagt ctttgataaa cggagaagag attagttacc cgctcagaat
    16621 tcgatgttga aaatcagcaa gctggcgtgt aggtcttttt ccgccaaacg gtaggcgagc
    16681 cgtacgccgg catttaattc gaccgcgagc tgtaaaagat taaagtcaaa atttaggtcg
    16741 agacccactg aattatattg ctgtttgaag tcactggttt ttcccacgtt gaaatcgccg
    16801 aaaaaggcac cccgtaggcg cttgaggtaa aacagcgaac ccagtgccca gtccggatag
    16861 gcgagcggca attcgtaggt agcgccggca cccgccaacc ggtcgttata aatatagtcg
    16921 tagcctttgg caaacggcac cagactggca aagcggtaat tgtcaggatt ttgcagttcc
    16981 actttggcat tgagcatcag gctatgatgt ttagccaaac cgggcaagta ataattccct
    17041 tggaaggaat acatctgact ttgataatcg ccacccagtg gagtatgttt gaaattggct
    17101 ttaacggtat agccggcggt cggcttgaca tcgcgaatcg ctggggtttg gtaattctgg
    17161 taggcaattt cataggaaaa agggacaaag ccaccgttac caatctcgta atcttcgact
    17221 gtggttttat cactgatgta ggtataagca ccactcaagc cgaggttcaa cttccggtta
    17281 aatacactgc tagaataatc cagcggcaag tttgcaccca atgagaatga cgtttcgttc
    17341 caagtctcag tggttaattc gtcaaaaagt ccaccggaat agctgattct cttgccgagg
    17401 ttaaatccgg cgctcaggat aggataaaaa ccggcatagc aaacttccga tccgaaagaa
    17461 aaggctttct cattcacatc gaaattcaag gcaaaattgg cggcggtggt attgagaata
    17521 tcattagaga ataatccgag attggcggag gtctccgaga agaaatagta ccagctatgg
    17581 aaattgaata aatgccgata gggatcataa tccgtaaccg gatacgattt ggtcgggatt
    17641 tttcccgctt cgaaaatatt accaccttgt tcctgagcaa ccagcgtctc ataatagcga
    17701 atatcgcatc gttcgaccgc ttccagcgcc tgccaacggg ctggctcgag gtgcattgcg
    17761 catacatcca gaccattaaa atcataatcg ctaaaaagca tcatcgagcc gtcggctgaa
    17821 accgccgggt aataggcgcc gtatttgcgt gaagtcactt gaaaacgacg accgctgtca
    17881 atttctaccg cataaatatt gtcaattccc gaccaagctg agttataaag cagatagtcg
    17941 ccgtaaaaaa ccggatattc gtcgaacgag tgacattccg cgataataat ctcttcagta
    18001 ttgttctcca gatcgtaaat cgccagtgac ttggaggatc cagattgttt aaaatagact
    18061 aattgctttc catcgggcga ccaggctggg ttcttgataa aaccgttatc cggattggat
    18121 aaagtcctga tttcttgacc atcattagag tcaagcaaaa ccaggttgca ttttctttcc
    18181 ggggtaaatt ttacagccgc gatcatctgc ccatgcggcg caaccgctgg cgaaaagtat
    18241 ttaccgttct tggtaagttt acgctcctgt ccgactaata aatcatagac aactatatcg
    18301 gaaaaaatcg cggcgcccca gcgtggatga ggcgtcaatt cttcccaggc aatcaaattc
    18361 ccggcagtcg aaaaccgatt ggcgctggcg attttggcta tttcagtttc ctgcccatca
    18421 gggttgatct tcactaaagt attgatgtca cctaagccat taattacggc gatgattgag
    18481 ccatcagtcg catattgcgg ccagcggtat tgtgtccaga ccttttttgg ttttaagtta
    18541 acttttttgg cagaggtgat ttccagtcct tcgatcggtt tgcaccagag tgaatcgagt
    18601 tcagtcattg tatcataata aagctcttta gttttgcgac cggtattgat tcggcaagct
    18661 ttcgagaaac caaatggatt ccaggagcgc cgagtcgagc tggttaatac gtgatcccag
    18721 atatttgcgc catactgccg tcgtccatga gttaccatta aaaagcccag atgataataa
    18781 ttcggataat aatccttgta cgagccaaga taggctttat cataagaata tagtttttga
    18841 ttgattaaca aagtccgaat cggtagatcg aattccggtt ggcaaccgcg tccagattca
    18901 gtcaaagcag tttccatagc gacggcatcg ccttcccaga accacatcgg cgcatgccag
    18961 aacgagagcc ccagaatcgc atattcaccg aacaaaaatc cagccaagcg cgtaaatccg
    19021 gaattgaagc gatcaaactg gaccatatgg cgtccttcat gggtagccag gagattatac
    19081 cattcaatat tgccggacat agcggattga tcgggagtcg taaaccattc acttttacgc
    19141 ggggcaaggg ttacataacc gttagcgacg gcactgcggt tggaaaggac taatggccat
    19201 ttaggactgt tcgctttcag tgttttacgt aaaggcaagt aaacatactc gagggtatta
    19261 gccatccgct ggctttccga ttcgatttcg gcgggaaaaa ttatccgata atgctgtgtt
    19321 tctatctgac gccagtgaat atctggcggc ggttggtcgg aaccgagcat ttgcgccaat
    19381 aggctattta ctaaaataaa aacggataga atagcaatca acaaaatccg tagaaaaatt
    19441 ctatctgtag taaatataaa tttcattaaa ccgtactatt atcaaattaa atttacgggt
    19501 agaatcggca atctcaaaaa atgccccagc tttaattcaa ttacaaaaat ttgaaaacat
    19561 tattatcacc aattagaaag ccagaggaaa tttctcttat cgagnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19681 nnnnnnnnnn nnnnnnnnnn nnnngaaccg atttttcggt ttgagatttt aacactgcaa
    19741 ttggcggtgc caccacgtat ttccggacca taagatggta gccagctaac ttcgtattca
    19801 tgacgcatac ccatctcagc taggacaagc ccgagactta aaattccctg accgccaaag
    19861 ccggcgactt tgatttctgc tgaaaaatta gtctttttat agggtgcgat cttttcttta
    19921 tctacggtga tcggttcaat atttaatata gaatgaagct gggaacgatc gattgttgga
    19981 cgttttaaat ctctactcgg aaattcattg gaaatgtctt ttttcactcc gagtggaaat
    20041 tgtttttgaa gtacttcgca tacccattcg cgactacgga tggctgacat cttgaggttt
    20101 gagggacagg gtgaaagcac ctcgataaag caaaaggcat tcttctcgat ctgaagtttg
    20161 agggctttac gaaccgcctg acgggcatgg agaattgatt tggtgtcacc aatcataacg
    20221 cgctcgacgt aaacaggggc tttgagagta gcgattattt ccgacatacc aatcggatag
    20281 ccctcgttat tagcatttct tccataaggc gtcgtcatag ttttttgccc gattaatgtc
    20341 gttggcgcca tttgccctcc ggtcatgccg taaatcgcat tattgacgaa aaaaaccgtt
    20401 atgccctcgc ctcggttagc ggcgtgaatt atttcggcgg tgccaattgc ggctaaatca
    20461 ccatcaccct gataggaaat tacgatggct tcggggtgag cgcgcttaag gccagtagcg
    20521 gcggctggag cacgtccgtg agctacctga atattgccgg tatgaaaata gtaataggca
    20581 aaaacactgc aaccgaccgg actgataaaa attgtgcgat cctgaacgcc gaaatcaacc
    20641 agcgcttcgg caatcaattt gtgcagattt ccatgtccac aaccgggaca gtaagtagtg
    20701 gtaagtttgt cggtaccggg cttgcgctca aacacatcat aaaaaatggc tgatttctgc
    20761 aatatgttat tcataccgac ctcatcagtt taagcaattc gtcgttaatt tccttttcgg
    20821 aaggcacatt gccgcccagc cggttataaa ggtacaccgg cttacgaccg gcgaccgcta
    20881 aacgaacatc gtcaaccaac tggccattac tcatttcaac gactaaaaag aatttggcct
    20941 gttcggcaag ttggctgatt ttctttttag gaaagggaaa aagagtgatc ggtctaagta
    21001 aaccgacctt gactcccaca tctcgtaaat tgtcaacgat agtctgcaga atgcgcgata
    21061 taattccaaa tcccaccaga acaatttccg catcgctgag actataatct tcaaaacgaa
    21121 cttcttgctc ttctataatg gcatattttt gctggagctt caaattatgg cgctccaaat
    21181 cgtccggatc gagttcaatt gaagtgatta gattatcgga agtctcagga gtacctttga
    21241 tcgcccagga tttttcggga aactgcttta ccggctcggg aaactcgacc ggctccatca
    21301 tttgaccgac aaaaccgtct gagagaatat aaaccgggtt gcggtattta tcagccagat
    21361 cgaacgccag catgccgaaa tcacacattt cctgaacgga attgggcgct agcacgatgc
    21421 atttatagtt tccgtgcccg ccacctttga caacctggtt atagtcgccc tgttcaggag
    21481 cgatattacc cagcccgggg ccccctcgca taacatctac gatgacacag ggcaattccg
    21541 aaccggcaca ataggaaatg ccttcctgtt tcaggctaat gcccggaccg gaggaagcag
    21601 tcataacgcg ctgaccagcg gccgccgcac cgtaaaccat attaatcgcg gcaatttcac
    21661 tttcggcttg caggaatgtg ccgcctgaca ccggtagata taaagccgcc gcgtgggcaa
    21721 tttcactagc gggcgtgatg ggatagccgt aataagcttg acaaccggct agcaccgctc
    21781 ctttcaccac ggcttcgttt ccttttaaaa attgtttagc catagtttaa tcctccgcta
    21841 tttaatggtt atgacctttt cgggtgacac cgggcgcaga catcccgcgc agagttttac
    21901 cttttcattg ccctctctgg aaaaaacttt ctgttcgcta ccgcactccg gacagaaagc
    21961 cttctcagtc actaaatccc agcgtttgaa aactgtaata gcacccagtt cgggacaatt
    22021 atagaagcag attccacagc cggtacagcc ttctccctta taagcggaag gatgataacc
    22081 gtgagtatta aatcggtcag tcaaatacaa aacctttggt ggacaggacg cgatacataa
    22141 ttcacaaccc ttacattctt cagagttgat gataacatgt ccacgcgtga ctgttttttt
    22201 cggttttgat tgggtttcat tcatatctgc tccattgttt aattatggtt tcatttcatt
    22261 gaaaacttgt ttgaatatag gaattgcggc ttgaatcacg gcttcgggta cagcataaga
    22321 aatccgaaaa tatcccggtc ggtagaaagc aatccccggt gtcgtaatta tcagccgatc
    22381 ctgcaaggcg aaaacgaatt taacatcgtc atcaatcggg catttaggaa agaggtaaaa
    22441 agtgccttcc ggtttgataa tttcataccc gattttcact aaagcgtcat aaatcatatc
    22501 acgccgtttc tggtataatt cgatagcgac ttgagcatcc agtactcgtc gaataactct
    22561 ctgcatcaac gctggcgcgt taataaagcc cagcacccga ttgcagaacg ccgccgcatt
    22621 gattaaagcc ggataatcct caattaatgg gctgatagct agataaccga tgcgttctcc
    22681 ggctaacgaa aggtctttgg aaaacgagga tgccagaatc gtatgccgat aatagcgaaa
    22741 tggagaacga tattggagtc cgtcaaaaat gattctctta taaggctcgt ccatcaataa
    22801 ataaatcggg cgaccaaatt cggcactttt ttcggtcagc aatttcccaa tctcacgaaa
    22861 aatgtcttcg ccgtaaacac gtccggtggg attgttggga gaattcagca gtagaacccg
    22921 agtacgcggc gtaattttcg cggcaatgtc agccggatcg ggtaatagtt ccgcatttgc
    22981 ctcggaaatg accaattgcc cgcggtgatt atctacataa aattcatatt ccgaaaagaa
    23041 aggggcaatt acgatgactt cgtcgccggg atcaagcaga gatttgagaa tgatgttaat
    23101 tgctccagcc gcaccgcagg tcataacgac ctgatcttcg gtaaacggca aaccactttc
    23161 tcgctcgagc aaattcgcca ccgccgcaca ggtctcggga taacccttat tttgcatata
    23221 gccatgcaca taagccccgg tacgagctga ttcttcgacc agaacgcgat taaaaatctc
    23281 cggagtaggc aaatccgggt tgccgagaga gaaatcgtaa attttatctt caccatactg
    23341 ctttttcaaa gcaattccgg tctcgaacat tttccgaatc cacgatgagc gggtcataga
    23401 ttgagctata taattggaaa tcggcatatc acctctaagt tttattttta ttggctgaat
    23461 ccggctccgt aacccaaatt ccggttttga ggtattcgtc aattgaatgc gcggcggctc
    23521 ggccggcgcc catagccaga ataacggttg cgccacctgt aacgatgtcg ccgccggcga
    23581 aaacgccgcg cttggaagtt cgtccgccct cgccgctggt aatgatattg ccccatttat
    23641 tgcattgcaa atcgggcgtg gtacgggaaa tgagcggatt tgaaccattt ccaatagcca
    23701 taatgaccag atcagtcggg atttcaaact cactacctgg aatgggaata ggacgtcgtc
    23761 gcccatcgga atcaggttca ccgagttcca tttgcagaca gcgggcggct cttacccagc
    23821 cattttcgtc aggcagaaat tcaatcggat tggtcaaagt tcgaaaaatg acgccttctt
    23881 ctttggcatg tttaatctct tccaggcggg ctggcatttc tacctccgag cggcggtaaa
    23941 caatcgtggc tttctcagct ccgagacgtc tggcggttcg gacggcatcc atagcggtgt
    24001 tcccgccgcc gacaactacc acctgtttgc ctatttttat cggggtgtca gcttcaggaa
    24061 attcataagc gcgcatcaaa tttacgcgcg ttaaaaattc attagcagaa tagactccca
    24121 tcaaattctc accgggaata ttcataaacc agggtaaacc tgcaccgaca cccaggaaaa
    24181 ccgcatcaaa tccagcaact tgaaaaagtt catctatcgt catagttttc cctacgataa
    24241 aattagcttc aaagcgtacc cccaattttc gcagattgtc aatttcgtat ttgagaatgg
    24301 ctttcggtag acgaaattca ggaataccgt agatcaaaac accaccgaat tcgtgcagag
    24361 cttcgaatac ggtgacgtcg tagccccatt tgataagatc actggcacaa gccagaccag
    24421 ccggtccgga gccgacaatg gcgacttttt tcccattgcg cggcgtacta agagaaactt
    24481 gaggttgacc gttctgccgt tcataatcgg cgacgaagcg ctccagtctg ccaatcgaga
    24541 ccggaccgaa tttgccgcga atgacgcagg ttttttcgca ttgttcctcc tgcaggcaga
    24601 cgcgtccaca gaccgcgggc agaaggttat cctctttgat tttgcgcgcc gctccgctaa
    24661 aatctcgctg aacaatcaac cggataaaat cgggaatttt gatgctaacc ggacacccgt
    24721 caatacaagt cggacgggga cattcaagac aacgctgagc ctcccgtatg gcagtctcag
    24781 cgtcgaagcc gagattaact tcattgaaat tatggatacg ttcttcgggt ggttgttccg
    24841 gcatttgctg acggggaatt gctaatcgtt cttttttgct gagatttgac gatgacactg
    24901 actttttcct tttcagaact aactattttc gctaatgact tttgataatc ggcattgatg
    24961 aagaacttta tccggttcgg tattcaaaat atcggcggag attttttcct gggcggcaaa
    25021 ttgtttctga cggtgagcaa gttcttcaaa attaacctga tgaccgtcaa attccggacc
    25081 atctacacac acgaaaacag tcttgccgcc gactgacgcc cggcaaccgc cacacattcc
    25141 ggtaccgtca atcattatac tattgagaga taccattgtc ttgatggcgt ggggacgggt
    25201 tacttccgat acggctcgca tcattgggag cggtccgacc gccagcacaa aattcggttg
    25261 aggtttacca gctataatgg tttttaatac atccgtaact aaaccnnnnn nnnnnnnnnn
    25321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25381 nnnnnnnnnn nnnnnnnnnn nnnnnattat ttcatcgcgg gtagcgccgg ctcgaatcgc
    25441 ttttggcaaa atgattttaa tggcttctgg cgacgggctg ttgatctgga tcgccagcat
    25501 tatcagatga cgggtttttt catcgaggtg atttccgagt gaattaatca ttttctggta
    25561 atttcgtaaa acttccttac gattctccat caaataagtc agtccgtctt tatcaagatc
    25621 ttcccatttg aaattatcat tcgtcatttc aatcctctgc caatttattt aatttcttcc
    25681 agccagccga atctatcggg gacgtcacca acttgatagg ctgttagata tttatacagt
    25741 ttagtcgtga ttggaccggc ttcttcgccg tcggcataaa atttatagag cttgccgcga
    25801 tgcccgagac tgccgatcgg tgtgatgacc gccgccgttc cacaggcacc cgcctcggcg
    25861 aagttttcaa tttcatcaat actgatgcgg cgctcctcca tattcaggcc aaattcttcg
    25921 cgtgctaccc taactaatga cagacgggta atactttcca gaattgattc cgacttgggg
    25981 gttgctaaac tgccatcttt aaaaacgaag aaaatatttg acgaactgat ttcttcgaaa
    26041 tatttatgtt cgacgggatc gagataaacg agttcattgt agccttcatc gtgagcgatt
    26101 tttagtggca agagcgaagc cgaataatta ccaccggctt tagcctgacc gacaccgtgc
    26161 cgggctaccc gatcataata ctcttcgact ttcaccttaa tagtctggaa tccggatttg
    26221 aaatagggac caaccgggca aacgaaaact gaaaagagat attcaggcgc cggtttgact
    26281 ccgagattat cgccaacccc gatttgaaac ggacgaatat ataaagatgc gccagtgcca
    26341 taaggcggga cataatccag gttagccttg accgtttcga ttatgccttt cataaataaa
    26401 tcttccggca cctcagccat cagtaggcgg gcggctgacc gccggagacg cgcggcgtta
    26461 tcccgcgggc gaaaaagcat aacccgtcca tcacgggtcc gctgagcctt cataccttca
    26521 aaaacttcct gcccatagtg caaacaggta gaaccttcct caatgctgat ttttttatca
    26581 gttactaatt ggccatcatc ccaatgacca tctctccaac gcgcctgaaa acgaaagggc
    26641 gtgtaggaat atttgaactc aagtttactc cattcgattg ttttcgggtc catcattttc
    26701 ctctttttta atccatttta ttagcaaacc tgaacggctt gcttaaggta atttaacctt
    26761 caaatgacac taatccttaa tttttttatc aaatgtaatg ccaccagtca gtataaattc
    26821 gctaatctag caggtaaagg ctggtttcat ttgatttgga gaataaatag cataatccta
    26881 aagtctatat ttttcctaaa actggcaaca accagccggc gatttattag ttttaggaat
    26941 gaatatttac ggagattttc cgatttctac gtatattcac cagagatttt tatgaagtat
    27001 taaaatttgg ttatgaattt gcgaacctac tagctcaatg caaagcagtt ttaaagtttt
    27061 gaactaaatc accgtaggag gtcgtaatgt ataaaaaatt atttattcca ggcccagttg
    27121 atgtccggcc tgaagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccta
    27241 ctttgccaga agtagttgat cagtatctct cgaccggtaa atacgatgtt ttcacgatta
    27301 ctcacaacga aacctcgacc ggtttgatga atccggtagc tgaaatcgcc gaagtcaaac
    27361 gcaaatatcc cgatgtttac tggttagtgg atgcggtttc ttctatgggt ggcattaaaa
    27421 ttccggtaga tgaactcggt attgacgttt gcattacctc gacgcagaaa tgtattggtc
    27481 tgccgcccgg aatggcaatc tgttcggtta gtcagcgagc gttggaacac gcgcgtaccg
    27541 taccgtttcg cggcgcctat ctcgatcttg tcgagctcta tgattatttg atcaaaaaag
    27601 attttcaata tccatccact ccttctctgt cgcacatgtt cgctctggat ttacagttgg
    27661 actatattct gaatgtcgag ggtctggaaa agcgttatgc ccgtcatcga gaaatggctg
    27721 aaattacgcg cgcctgggct aaggaaaact ttgccgtctt tccgcaaccc ggctacgaat
    27781 cagttacctt gaccaccatt accaataccc gtggtatttc aatagccgat ttgaacaagc
    27841 ggcttggtga agccggttac cagatttcca atggctacgg cgaactgaaa gaaaagacct
    27901 tccgcatcgc ccatatggct gaacgcaccg ttgatgaatt aaggacttta ttgaacctca
    27961 ttgacacgat aaccgctaag atgtaaaatt cttggccaga aagaaaagta tgaaaatatt
    28021 aattgccgac ggtctggatc aaaaagccca aaatattcta aaagaagccg gactggaact
    28081 ggttgtcaaa cattacgaag ccgacgatct gatcaaagtt attcaagaat ttgatggtat
    28141 aattgtgcgc tcagctacca aagtacccca agttgtgatt gacgctggta aaaaccttaa
    28201 attcattgcc cgggccggca caggtataga caatattgat catcaatatg ctcgggctaa
    28261 aggaatcgcc gttctgaatg cacccggtgc taactcgcat tcagtagcag aattagtctt
    28321 tgcccacctg cttgctctgg cacgttttat cactcccgcc acgatttcct tacgcgaagg
    28381 tcgctgggaa aagaaaactt tcggtaatgg cattgaatta gccggcaaaa cgatcgggat
    28441 aattggtttc ggacgcatcg gtaaaatcgt agctcactta gcaattgcct ggggaatgca
    28501 cgttcttgta tatgacgtcg caacaatcaa taccgaacct accgtaaagg tagtcgattt
    28561 tgaaacatta ctaaaggaat cggatttcat tactctccat actcccaaaa ccgatcaacc
    28621 gatcataaac gaaaaagaga tcgctatgac gaagcgtggc gtctatatta ttaattgtgc
    28681 ccgcggcaat gctattgatg aaaaggcact gttagcaggt ttgaattctg gtcaagtcgg
    28741 cggtgccggc ctggatgttt ttgccgcaga accaccgcaa aacatggaat tacttcacca
    28801 tgcgcgggtc tcctgcactc cccatattgg cgcgagcact ctggaagctc aggaacgcgt
    28861 cggcgccgaa atcgctcacc gaatcgtgga gtacatcgga accctgtagg aatttatatg
    28921 gtaaaaatca aaccattccg tggcatccgg ccggttaaag aattagcgga caaaatcgcc
    28981 gcccctcctt atgatgtcat aaattttgaa gaagcccgaa gtcttgccgc cgacaatcca
    29041 tattcttttc tgcatgtcag cagagcggag atagatttac caccggaaac tgatccctat
    29101 gatgaaaaag tctatcagaa agctcgcgaa aatttagagg caatgcttgc caagggctgg
    29161 ctctttcagg atgcgaccga aatgctatat atctatcgcc aaataatgga taaacatagc
    29221 cagtatggat tgattaccac cgctaacgtc aatgactatc ttaagggaaa aatcaaaatc
    29281 cacgaa
//
LOCUS       DAOOMU010000017        27949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1503_length_27949_cov_17.149817, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000017 DAOOMU010000000
VERSION     DAOOMU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 27949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27949
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1503_length_27949_cov_17.149817"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLI55_02880"
     CDS             complement(<1..836)
                     /locus_tag="PLI55_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99812.1"
                     /translation="MKKILQRFFLICPKTGKVRGIKPLSGWSKLLFPITGLLALIWVV
                     VRVLPKPTRATYPCMRVAMPLASGFIVYLIGVIASATAFMKAKKMLAKSRYLLATGFV
                     ILGLLASLLIFHNSRETAYATYATIEHEANIPMGTAKGIFPGRVVWVYNPDATNENCV
                     PDSTVSSWWGSGYTTYRAGKAWFLPQNNNQAVVDSMVSSAIQSLTGATSDSAAWVAIF
                     KYHNTTREKGAVNYSPGEKIFIKINATSGWGGNYSTTDLSCAKNSSYGISETSPAVVM
                     TV"
     gene            complement(909..3302)
                     /locus_tag="PLI55_02885"
     CDS             complement(909..3302)
                     /locus_tag="PLI55_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N'-diacetylchitobiose phosphorylase"
                     /protein_id="HPA99813.1"
                     /translation="MQFGHFDDKNKEYVIDRPDTPRSWSNYLGSTEYGAIITNNAGGY
                     SFFQSAAQGRFMRLRFNAIPMDQPGRYFYIHDLDKHDYWSTSWQPVGKPLDKFKTVCR
                     HGTAYTIIESEYDNILTETTYFVPLNRHFECWLLKIHNRDNVTRRLRLFTFIEYTNNW
                     HVWQDTFNLQYTQFILRMNVVGNIIDHGINVNLTDSSGDLWLHDGNKHTFIGVVGAEV
                     SGFDTDREIFLGGYRSYHNPIVVEKGECLNSIAVGDNGVGVLQIDLELKPGESRELAV
                     LMGIGRAEAEGRKVLSEFQNLDNIHIEFEKLKQHWHQRLQGFLVQTPDSEFDSMTNVW
                     SPFNCLITYAWSRAASLIYSGERDGLGYRDTVQDLLGVLPLIPEEAGERLELMLTGQV
                     STGGAMSVVKPFDHHPGREIPPKEEEYRSDDCMWLFNTVPAYVKETGDLSFFDKVLPF
                     ADEGEATVLGHLRRAIEFNLERTGTHGLPCGLYADWNDCLELGQRGETIFVAFQLRYA
                     LKTYIEICELLERLSEKKWAVEQLQKLDENLRKYAWDGQWFLRAYRYDGLKYGSHTDE
                     EGSIWLNPQSWAVISGFADKDEAETAMQAVYERLATEHGLMILAPPYIKTDHRIVKAP
                     LFNAGMKENGSIFSHTQGWAIIAETMLGHGDRAYQYLRAYLPAAYNTRAEIRQIEPYV
                     YCQFTNSRYSPRAGASRLPWLTGAAAWSYYTATQYILGIQPDYFGLRIDPCLPSNWKE
                     IQITRRFRNKMFNILIKNPNGTQKGIRKLIINNQDLTGNLIPLDIIQNENKVVVEMQ"
     gene            complement(3327..4784)
                     /locus_tag="PLI55_02890"
     CDS             complement(3327..4784)
                     /locus_tag="PLI55_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA99814.1"
                     /translation="MAQEITANNSTTQVRDRLPVLTKICYGLGTGLDMWGFWLYPGVA
                     FAVFNMYLGVNPMLVGLALTLIRIYDAFTDPIAGWISDNFRSKHGRRRPFILGAGVIS
                     GLGLPLLFLVSPAWADQTFLGLSVVFWYMLLSNLIYIPIISLFTVPYNSLGNELTPDY
                     EERTSVMTYRSVMQKIFEVGNFYALRFTNLAWFLIPGTGRKNTLRGMQVYTSIIGVLM
                     AIFAIIIFFRVKERYYEKVVVKTKERIKLASSFYETLKCQPFRMMLLLGGSFTLGTSM
                     VGTLGYYTTVHYVCGGNNIVGDNWNFWMGIAFMIGGLLGGPILNRVAYYLEKRKAVIV
                     AAIIGIIGYGGSWFLYTPLVPWLQTIASGLMGLSSAGLWMLHGSIGADIIDYDELNTG
                     KRREGSFTGCSSYILKLGNSLGGFLAGSVLSFARFDPSLAVQAPETIFWIRAMLACIP
                     VVGLILVIFFITRVNLTRQKCAEIRSALEARRGTV"
     gene            complement(4832..5821)
                     /locus_tag="PLI55_02895"
     CDS             complement(4832..5821)
                     /locus_tag="PLI55_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HPA99815.1"
                     /translation="MKYRIVGNSIPNLPWEERPEGCSDIVWRFSKNPLIGWNPIPKAA
                     RIFNSAVISYKSEFVGVFRADQKNGRATLFSGKSHDGLRWEIGPDPINWVDEQGNPKP
                     LSYGYDPRLVKIDDIYYIVWCDDLHGASIGLGKTQDFKTFTRMPNPLMPFNRNGVLFP
                     RKVNGKYLLLSRPSDSGHTPFGDIFLSESPDLIYWGNHKFVMGKGGMGWWQGTKIGAG
                     PIPIETSEGWLLFYHGVSTTCNGFVYSFGAVILDIDEPSKVLYRTRDYLLTPEKPYET
                     TGFVPNVVFPCANLCDTATGRIAIYYGAADTYTALAFAQTDELIDYIKNNSEV"
     gene            complement(5850..7259)
                     /locus_tag="PLI55_02900"
     CDS             complement(5850..7259)
                     /locus_tag="PLI55_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA99816.1"
                     /translation="MAKYLTLREKIAYGFGDLASVLYWQTFMLYFTYFYTDVFLIPAG
                     VAATMFLVSRVWDAVNDPLMGIIADRTNTRWGKFRPYLLWFCVPLAVAGVLTFSVPNF
                     GMTGKIVYAYVTFILIMMLYTAINIPYTALLGVISPDSDERTSVSSIKFLFAFTAGVI
                     VSATLLPMVKALGKGNEARGWQLSFVIYGIAAIIFFLIAFKGTRERVHPPKSQKSSIK
                     KDLYELVTNSPWLILLFTTITFILFVAIRSSVTVHYFKYIVGTREVSLPFFKNKTWDF
                     ETLTSVFNTVGQISSFIGVLLVSWFAKKVGKKPAFMIIFVIAIFSTSIYYFLSPEQIG
                     LIFLFQIIGSLTGGPLSVLIWAMYADSADYAEWKNGRRATGLVFSASTMSQKFGWAFG
                     AFVALNLMSRLGFMPNQAQTPQSLRGLLLLFSLIPAAFGILALIIVSFYPLNNQRMAV
                     IGADLKERRAQSGETIAPA"
     gene            complement(7362..9800)
                     /locus_tag="PLI55_02905"
     CDS             complement(7362..9800)
                     /locus_tag="PLI55_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HPA99817.1"
                     /translation="MKFGHFNDQDREYIITTPETPYPWINYLGSQDFFSIISHTGGGY
                     SFHRDARLRRITRYRYNNSTLDDNGKFFYINDNGDVWNPGWKPVKKALDFYECRHGLG
                     YTRISGQRNDILAEVLAFVPMNFDGEVQQVTLKNTGNSVKEIKLFSYVEFCLWNAWED
                     MTNFQRTFSIGRAEAIGSTILHFTDYKDRRNHFAFYSVNAPIDGFDCDKESFMGLHNG
                     PHEPVAVFEGKNRNSVAFGWSPVAVHQIEVKLNPGESKSFNFILGYVEYPETEKFTAD
                     GKINLTKVRQMQSRFESPESVTKAMNDLREFWDGLLSVFQVQTGEEKIDRIVNIWNQY
                     QCMVTFNMSRSASYFESGIGRGMGFRDSSQDLLGFVHQIPERARERLLDIAATQLPNG
                     GAYHQYQPLSKRGNYEVGDNFNDDPLWLICGVVAYLRETGDFAILDESVPFDNDPNNQ
                     ASLFEHLKRSFYHVVNNLGPHKLPLIGRADWNDCLNLNIFNTDPDQSFQTGPLKTDGK
                     TAESIMIAGAFVLYGQEFVELCEFLGKMDEATEARQHIDEMKKSIDRFGWDGEWFLRA
                     YDAFGRKVGSQENEEGKIFIESQGFCCMAEIGKEDGRMQKALDSVKKYLDCEFGIVLQ
                     NPAYTKYYIELGEISTYIPGYKENAGIFCHNNPWIMIAETKLGNGEQAFEYWRKICPS
                     FLEDISDLHRTEPYVYAQMIAGKDAYKPGEAKNSWLTGTAAWNFLAVSQYILGIRPQF
                     DGLLIDPCIPSDWKSYTVRRLFRGKNLNIEVRNPAGVQKGVRRITLNREALSNNLIPC
                     SKLKNTNEVIVDMG"
     gene            complement(9867..12002)
                     /locus_tag="PLI55_02910"
     CDS             complement(9867..12002)
                     /locus_tag="PLI55_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPA99818.1"
                     /translation="MRKTYLGGFLFFLILLNLFATNNRLFSQEFNRDMETFQPLAYAE
                     ADKLAESILAQMTLDEKIALIGGDKYFFIRALPRLNLSEIYMSDATQGIHIRQSFNEI
                     DLSNYQPEKSTAFPCPISLAATWNRELAYQYARTIGEECKAANIGILLGPGMNIYRQS
                     QCGRNFEYFGEDPYLAARMIENYVQGVQSTGTVATLKHFLANNTEYYRKKSNSVVDER
                     TLHEIYMPAFKAGIEAGAKAVMTSYNLLNGEWCGESAYVINYLLRNQLGFQWLVMTDW
                     WSVYNGEKLAKSGQNLEMPFTIALENVHQLLEDNKVQVSDIDRMAQSILRTCFAMKLY
                     GRSRTAVEDSFYEAHEKVALQTAREGIVLLKNENNILPVKEQVKNILVTGDYVKKLAV
                     GGGSAYVEGYNNRQMISELQKVFGDRIKYVKNPTVKQIKSAQLVLCNVGTSDSEGWDR
                     PFELPEDQEQKVLKCVNNNPNTVVIVTSGSGIRMTGWNEKARAIIYAWYPGQIGNQAL
                     AEILIGKVNPSGKLPITIEKEFKDSPGYGYIPEGESLYFDWNGEGERAHPVYDVRYKE
                     GIFIGYRWYESQKIEPLYPFGHGLSFTSFSYSDLQVTPGKFSEVDTVSVTFSVKNVGK
                     YQGAEIVQLYVSDLESSLPRPAKELKGFQKIVLEPGEIKTVTLKLSQKDFAFWSPAIK
                     NWTTEKGKFVILIGSSSQDIRLSKEIELL"
     gene            complement(12046..13404)
                     /locus_tag="PLI55_02915"
     CDS             complement(12046..13404)
                     /locus_tag="PLI55_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPA99819.1"
                     /translation="MNSVQRHNRHKCKIITVFWLIVILPILIQCEQNNESDQIGGSDL
                     TFVEEHGNLTVSGNRIIDQNGDPIVLRGMSLFWSQWMGKYYNYNCIKWLRDDWKCTVV
                     RAAMGIESGGYLNNPASEKAKIVTVIEACIDLGIYVIVDWHDHNAHEHQAQAIAFFQE
                     IATDYGTYPNLIYEVFNEPEQDNWKTVVKPYLNAVVDSIRAIDPDNLIVVGTPNWSQD
                     VDIASQNPLAQNNIAYALHFYAAYPYHKQTLRNKASTALNNGIALFVTEFGTVMNTGD
                     GPIDTAETRLWMNFMESNQISWCNWSIADKNETSAALKPGASANGGWSVSEITESGLL
                     IRDYIIRGNTKQPNLVRKTRLIPAGFALAQNYPNPFNSSTQISFELPVSAGVNLGVYD
                     LRGNLICTLLNKVFPAGRYQTTWETNGAIDEIAGGVYILRFLADSEIGTTQISRKLSL
                     IK"
     gene            complement(13512..15248)
                     /locus_tag="PLI55_02920"
     CDS             complement(13512..15248)
                     /locus_tag="PLI55_02920"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPA99820.1"
                     /translation="MRFKTITSSLIVCIAITTVLTAQNLIGPNWKFQPGDDLAWADPQ
                     YDDASWNSIIAGRTWEVQGYENLDGFAWYRYSVIIPSTLKEKAVKHGGFILRLGRIDD
                     SDATYFNGELIGQMGGFPPNYIGAYGLDREYIIPVELVRWDQPNVIAIRVYDAGGGGG
                     LYYSAAELNVIGLSDQLKIEIALPQKDHVIKNCSEIVLPVIMRNESNESIEGILRLAI
                     NSDFGDKIYDKELKITMKNNSAKKYSYNLKKLQPGFYLGAVSFESLGWNKLHKFAIAV
                     DPEKVISPVDAQPDFDAFWEQAKAELATVDPQFKMIRQDELCTAERDYYLVEMRSLGN
                     ILIRGWYSVPKNPGKYPAFLQVQGYGTNMQPTNLITEDGFISFGLNIRGHGNSRDEID
                     PGFPGYLQYHLENKYEYIYRGAYMDCVRAVDFLCSRPEVDTSRIIVDGQSQGGALSFA
                     TAALDDRIRLCVPGVPFLSDFPDYFKVAIWPGNEFKDYVNNHPETTWEEVFRTLSYFD
                     IKNLAPKVKAPVFMLVGLMDETCPPHINFAAYNNLQCPKEYIIYPYSGHGLPAENYQA
                     RMNWVRQQLNLK"
     gene            complement(15417..16187)
                     /locus_tag="PLI55_02925"
     CDS             complement(15417..16187)
                     /locus_tag="PLI55_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPA99821.1"
                     /translation="MLLTLISWNVNGIRAVYKKGFLDWVKATQPDILGIQETKAEESQ
                     LPPDLAQISGYRSYFSSAIRKGYSGVALYSRLEPVSITQKFGIEKFDTEGRLLIADYD
                     QFVLFNVYFPNGQMSEERLRFKLEFYDAFLDYVDRVNVSGRNIIIGGDVNTAHTEIDL
                     AHPAANSNISGFLPEERAWLDKFLSHGYYDTFRLFNNEPGNYTWWSPFTNARERNVGW
                     RLDYFFVNKRFVANVRAAEILADVAGSDHCPVSLQIEI"
     gene            complement(16191..17486)
                     /locus_tag="PLI55_02930"
     CDS             complement(16191..17486)
                     /locus_tag="PLI55_02930"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPA99822.1"
                     /translation="MKKIKYIVVGALLVIVSQGYSQLYMNPRSISFADAYATQARGGD
                     VIGWNPANLGLNDNPKFQFNFGIIPIVPFPALQISNNTISTHLLNNHIFTGDYLDDDA
                     KEELLSHFPDGGLNFNPLVQMRFLNFSVNSWAFTLGAEVTGKVVAPKSLFRLALFGNE
                     FEEPIDLSDTDLDLQSVVTLGIAHGWQIDNVPYLSNYVDKFSVGIAVKLLAGAGYSGF
                     DDLTANVTTYKDRIILEGDLKGEYGLGGAGLAIDIGAASVINEKMTANLALNNVVGFL
                     NWGIGEAGKAEYSFYADIPSSDFGDIDSIMDESLRKDTTYTVSGIRTNYPAYMIMGFE
                     YRVLQNLRLFVNYRQSFSNDMASTTTPAFSFAAEFLPANWLPIRLGIGVGGNDKIKWG
                     VGSGMTFKHYRLEWGFAQTGGFFNHGKGLAFCLDQSLVF"
     gene            complement(17491..>20448)
                     /locus_tag="PLI55_02935"
     CDS             complement(17491..>20448)
                     /locus_tag="PLI55_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99823.1"
                     /translation="MQKFKIIIIAGVAVLFSFCDLKIPSESDYPTWSVNLNVPLLNET
                     VTVNDLLEDSLIVKLPYGTAGDSIFAYEDQIPIEEVRVGNKLNIDDITQSIQQGVDEV
                     NIAASDKKYSSGFDEVGVEPVSEQNSNLIGDIHLADTDVESTAPILFSEIVDMTGIAD
                     GASKTIAISTPFPTINRQIVFNNFNNANFIGGFLEINIRNNLVVELGAPVYVRLLDGS
                     GNPIVGTDGDSAKAIWTEGLASGQSSKKTINLTNKTLPGTIIVEVSGVICGSGSQIVT
                     KNSATLNSSFVVEVQAKDISVSSAEAIVPEQTIDTTGTIEFAEDEPNKVETAVIKDGH
                     LKISIQNDLPVSANLELTIPSLKTVSGGSDFYQVIPMPAGQIVIQEYLLTDHKLEMDI
                     DEQEVNYSYIVRTISTDPEMVDISSSGQVNVNIDMYGENSESDVTFSEIKGIVEPQNI
                     KDSGEINTASDARISTAQIASGGMTFSIENGINIGTTGIPHLVLTFPEILTTAGTPVT
                     IESDIPGGQTTINYDLSNCLIQPLSESVTADSVRQYITYKSLVTTSSGEIAEYSLIDS
                     IKFDINTSEMTFSSVTGYFDQEAIVANEVLELEDKTKLETAQISNGNLILTFVNNIGV
                     FADVHFTINEIVHRTDGTPLERIIHLPNSSQPVIETIPLDEYNIQLPFTDLTTNQEIH
                     YTSRVSIPSDREMTITFGQEINVDVLLTEVEFASVSGYIDTVQVDIEPSENEVNALPE
                     EFDGINLKNVEMAIEFNTDIGVPVELNLFISSFNDEGESVRREIHQVITENPRVVIPD
                     AEELINIKPKKIVTSGYALVYGTGQVDTAQYVSGNISISVPFEMEITEDAKMEFDPEL
                     VEEDIPEEIESAILYAEVENGFEIGGKLILLAAQDTLLFDVAPPYGPDTLAVINVHPD
                     SSFREVVELGEKQIALFQDSVYVKTQFNLVGRTDEMGNPIPSRFMKGDELKILLYGTI
                     NGLIDFADRKEED"
     assembly_gap    20449..20548
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20549..>21824)
                     /gene="dxs"
                     /locus_tag="PLI55_02940"
     CDS             complement(<20549..>21824)
                     /gene="dxs"
                     /locus_tag="PLI55_02940"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPA99824.1"
                     /translation="FTGGLAYEGLNNAYNINGQFLVILNDNEMSISRNVGAMSNYFTR
                     IVTSPSYLNFKNQVWNSLALLPRGTNFFRKAGRKLLESFKNFLAPGIIFEEFGFRYYG
                     PVDGHNIQQLVNTLNNIKDLKYPVILHIITQKGKGLASAEIDPIKYHGIGPRKDVNAC
                     VSTPTAPPFLNAFSKIACEIGEKYPKSIFITAAMAEGTGLVEFARRFPMRYFDVGIAE
                     GHAVTFAAGLAACGFRPVVAIYSTFLQRAYDNIIHDVALQNLPVVFVLDRGGIVGEDG
                     PTHHGAFDLSYLCTVPNMIVAAPRNGTELRSLMFTALEQNDAPFAIRYPKDSYVEFDD
                     QAPAVPYQIGKWEIIQNGQDVAILSVGVMTNNAIEAACLLKPKNIFPTIVHARFVKPL
                     DTQMLSEIASKHRIIITIEENSIVGGFGEQVAR"
     assembly_gap    21825..21924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21925..22492)
                     /gene="pdxT"
                     /locus_tag="PLI55_02945"
     CDS             complement(<21925..22492)
                     /gene="pdxT"
                     /locus_tag="PLI55_02945"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPA99825.1"
                     /translation="MNNKARRVGVLGLQGAFQKHLKIINSLGAEAIVIRYKEQIEKCD
                     GLIIPGGESTTMSRLIDEMDLRESLEKFPGMIFGTCAGAILLSTNGTDPLVKSLGRSS
                     VKVKRNAYGRQVESFVENVDLTFSDKPFKGIFIRAPKLDHLDSSVEILGHLNGDVVLA
                     RSGNNLMATFHPELTDDFRIHQYFLNHLQ"
     gene            complement(22526..23407)
                     /gene="pdxS"
                     /locus_tag="PLI55_02950"
     CDS             complement(22526..23407)
                     /gene="pdxS"
                     /locus_tag="PLI55_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPA99826.1"
                     /translation="MDKGSFEVKVGLAEMLKGGVIMDVTTPEQAKIAEEAGAVAVMAL
                     ERIPADIRAAGGIARMSNPKVIKAIQDTVSIPVMAKCRIGHFAEAQVLEALGIDFIDE
                     SEVLTPADEENHIWKHDFKVPFVCGCRDLGEALRRIGEGAALIRTKGEAGSGNIVEAV
                     RHMRRVQSEIRKLTTLSDDELMAEAKRLGAPFELVRQIAKTGKLPVPNFAAGGIATPA
                     DASLMMQLGAESVFVGSGIFKSADPAARARAIVIATTYYNNPEKIAEVSAGLGEAMRG
                     LDLAEIPENERLAKRGW"
     gene            complement(23451..24290)
                     /gene="ispH"
                     /locus_tag="PLI55_02955"
     CDS             complement(23451..24290)
                     /gene="ispH"
                     /locus_tag="PLI55_02955"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPA99827.1"
                     /translation="MALKVNISATVGFCAGVQNAVKKAQTAAQKFGRVAMLGDIVHNE
                     KVIRELTDLGVEVVQNLDQIEPTVPVLFRSHGTPRSIWDEAHQRGLRIIDATCPLVRK
                     IHNEVQMLAKEGRQVIIIGDKNHEEVSGIASQINDPIIVSSPEEAERLPKMKKIGVVA
                     QSTQMIENVSAIVAILAAKTDDLRFMNTICRPTRQRQEQIRELALANDVMLVVGSKNS
                     ANTRRLAEIAKKLNTNTHHIENAEQINPDWFINKTSVGVATGASTPVEVVEEVVKKIT
                     NFD"
     gene            complement(24290..24850)
                     /gene="dcd"
                     /locus_tag="PLI55_02960"
     CDS             complement(24290..24850)
                     /gene="dcd"
                     /locus_tag="PLI55_02960"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HPA99828.1"
                     /translation="MSVKSDRWIEEMALKHKMIEPFEKSQISSGVISFGVSSYGYDMR
                     VADEFRIFHGRKDTVLDPKRITMDLFIDYKGAVCTIPSNSFVLGRSLEYFRIPRNVIA
                     VTSGKSTYARCGIFVNVTPLEPEWEGFVTISIANLAPVPVKIYANEGVAQVIFLESDE
                     ACRISYADKKGKYQAQQNITNAKIKE"
     gene            complement(24852..>25853)
                     /locus_tag="PLI55_02965"
     CDS             complement(24852..>25853)
                     /locus_tag="PLI55_02965"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPA99829.1"
                     /translation="MEKYNWQKIVEKIKESDKILLTTHHNPDGDGLGAEAALYHCLKR
                     LGKSPFILNSSPLPPEYSFLNEGEIFHTFPNELDQVELTDFDLLMILDASHTERLGAL
                     GPVLKSLNIPTICIDHHPFNNCLPENISVIDDKAPATVALVFELLKIIAPQAIDQKVA
                     EALYVGLMTDTGSFRFENTTAETMLLAAELLRYNVQPGLIYHYVYENYRPERMKLLGM
                     VLQNVQYEFENRLAWFCVTRAQVAAAGAYLDEVDGFTDVVRSIRGVEIAAMFLEERDN
                     RTRMNFRSKGRYPINNIARQFGGGGHPYAAGLVMDVGLEEAKETVLPALRKVFLDEG"
     assembly_gap    25854..25953
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            25972..26352
                     /locus_tag="PLI55_02970"
     CDS             25972..26352
                     /locus_tag="PLI55_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPA99830.1"
                     /translation="MTKRSQIYKCEICGNIVEVLHEGAGELVCCGKPMKLIVENTTDA
                     AREKHVPVIEKIADGYKVSVGSVLHPMIDVHYIEWIELDTDGQVFRQYLKPGDTPVAI
                     FKVSAEKLTAREYCNIHGLWKGTL"
     gene            26352..26840
                     /locus_tag="PLI55_02975"
     CDS             26352..26840
                     /locus_tag="PLI55_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPA99831.1"
                     /translation="MLSPKLLTAFNDQIKHELESAYLYAAMNAWFKSENFDGMAHWME
                     VQTKEEINHARKFYDHIFERGDVVELQPINILAKKWASPLDAFKAAYEHEQFVTGKIN
                     DLMTMAIADKDYAAQEFLNWFVAEQVEEEDSTLKIVQLLKRIGDHGQALIMVDRELGK
                     RE"
     gene            26857..27339
                     /locus_tag="PLI55_02980"
     CDS             26857..27339
                     /locus_tag="PLI55_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPA99832.1"
                     /translation="MNFNIDEVFEIAEQIEVNGSRFYRQAAELSQLAEHKNMLLKLAE
                     MEDQHQKTFAGMREKIKQTKQNLSYDPNQEAALFLQAFASGYVFDPTADSSALIREQK
                     SIADILHLAIGLEKDSIVFYLGIEKILLNEDDIVHVRAIIKEEMGHITLLARKLDELN
                     "
     gene            27486..>27949
                     /locus_tag="PLI55_02985"
     CDS             27486..>27949
                     /locus_tag="PLI55_02985"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HPA99833.1"
                     /translation="MAIRIFVTGGTFDKEYNELTGQLYFKDTHLPEMLILGRSRVEVE
                     IRTLMMIDSLEMTDDDRLLIAHHCQKAAEDRIVITHGTDTMVETARLIAQNVSGKTIV
                     LTGAMIPYKFGSSDGLFNLGSALAFVQTLSPGVYIAMNGRFFSWNNVRKNKKT"
BASE COUNT         8061 a         6453 c         5462 g         7673 t
ORIGIN      
        1 aaaaccgtca tcactaccgc cggtgaagtc tcagaaattc cgtagctcga gtttttggca
       61 caggacaaat cagtagtgct gtagttacca ccccagccgc tggtggcatt tatcttgatg
      121 aaaattttct caccaggtga ataattaact gcgccttttt cccgagttgt attgtgatat
      181 ttgaatatcg caacccaggc ggcactatcg ctggtcgcgc cagttaaaga ctggattgct
      241 gaagaaacca tactgtcaac taccgcctga ttattattct ggggcaaaaa ccaggctttt
      301 ccagcacgat aagttgtata accactcccc caccaagaac taacggttga atccggtacg
      361 caattttcat tggtagcatc aggattataa acccagacaa cccgaccggg gaaaatgcct
      421 ttagccgttc ccatcggaat attggcttcg tgttcaatag ttgcataagt ggcgtaagca
      481 gtctctcggg aattatgaaa aatgagcaag gaagccaata atcccagaat aacgaatcca
      541 gtcgctagga ggtagcgcga tttggctaac atttttttgg ctttcataaa agctgtagcc
      601 gaagcaatta ccccgattaa ataaacgata aatcccgaag ccagcggcat agccacgcgc
      661 atacaaggat aagttgcacg agtcggttta ggaagcacac gaactaccac ccagattaat
      721 gctaacaaac cagttattgg aaataataac ttcgaccaac cactaagcgg tttaattcct
      781 ctgaccttgc cggtttttgg gcaaatcaaa aagaatcgtt gcaggatctt cttcatcagt
      841 ttctctcttt cagattgtta gtgattatca aataatttta ttctgcattt attagcaaaa
      901 taaaaatttt attgcatttc tacaacaact ttattttcgt tctgaattat atccagcggt
      961 attagatttc cggttaaatc ttgattgttt ataattagtt ttcgaatgcc tttttgagta
     1021 ccgttcgggt tttttattaa aatattaaac attttattcc gaaaacgcct cgtaatctga
     1081 atctctttcc agtttgaagg aagacatgga tcaatccgca aaccgaaata atccggctga
     1141 atgccgagaa tgtactgagt ggcagtataa tacgaccagg cggcggctcc ggtgagccac
     1201 ggcaaacgtg aagcccccgc gcgtggacta tagcgactat tcgtaaactg acaataaaca
     1261 taaggttcaa tctgccggat ttcagctctg gtattataag ccgccggtag ataagcccgt
     1321 aaatactggt aagctcgatc gccgtgtccc aacatagttt cagcgattat cgcccaaccc
     1381 tgagtatgcg agaaaatcga gccgttttct ttcataccgg cattgaaaag cggcgctttg
     1441 acgatgcgat ggtcggtttt gatatacggc ggggctaaaa tcatcaaacc atgttcggtt
     1501 gccaagcgct cataaacagc ttgcatcgcc gtctccgcct catctttatc ggcaaacccg
     1561 ctgattaccg cccaggactg tggattgagc cagatcgaac cctcttcatc agtatgcgaa
     1621 ccatatttca gaccatcata gcggtaagcc cggagaaacc actgtccgtc ccaggcatat
     1681 ttgcgcaaat tttcatccaa tttttgtaat tgctccactg cccacttttt ctcactgagt
     1741 cgctcgagca gttcacaaat ttcaatataa gtctttaggg cataacgcaa ttgaaaagcg
     1801 acgaaaatcg tctcgccacg ttgaccgagt tccagacaat cattccagtc ggcataaagt
     1861 ccacagggca aaccgtgagt accggttcgt tccaaattaa attcaatcgc tcgtcgtaaa
     1921 tgaccgagca cggtggcttc gccttcatca gcaaacggta aaactttgtc gaaaaaagac
     1981 aaatcgccgg tttctttaac ataagccgga acggtattga ataaccacat acagtcatcc
     2041 gaacggtatt cttcctcctt gggcggaatt tcccggccag gatgatgatc aaacggcttc
     2101 actactgaca tagcgccacc ggtggaaacc tgaccagtca gcattaattc gagacgttca
     2161 ccggcttctt caggaatcaa aggcaaaacc cccagtaaat cctgcaccgt atcacgatag
     2221 ccaagcccgt cgcgttcacc actataaatc aagctcgcgg ctcgcgacca ggcgtaagta
     2281 atcaaacaat taaatggact ccagacattg gtcatactat caaattcgga atcgggcgtt
     2341 tggactaaaa atccctgcaa tcgttggtgc cagtgttgct ttagtttttc aaattcaatg
     2401 tgaatattat cgaggttctg aaactcgctc aaaaccttcc gcccctcggc ctctgccctt
     2461 ccaataccca tcagaaccgc aagttcgcgc gattcacccg gcttgagttc aagatcaatc
     2521 tgcaaaacgc ctacgccgtt atcaccaacg gcaatggaat taagacattc gcccttttcc
     2581 accacaatgg gattatggta actgcgatag ccaccgagga atatttcccg gtcggtatcg
     2641 aaaccgctga cttctgcgcc cacgactcct ataaaagtat gtttattgcc gtcgtgaagc
     2701 cagagatcgc cagaactgtc cgttagatta acgttaatcc cgtggtcgat gatatttccg
     2761 actacattca tcctaagaat gaactgagta tattgcaaat taaaagtatc ctgccatacg
     2821 tgccaattat tggtgtattc aatgaaagta aagagacgca gacgtcttgt aacattatca
     2881 cggttgtgaa ttttcaataa ccaacattca aaatgccggt tcaaaggcac aaaataagtc
     2941 gtctccgtca aaatattatc atattccgac tcgataatgg tataagccgt cccgtgccga
     3001 cagacggttt taaatttgtc aagtggtttg ccaaccggtt gccaggaagt cgaccaataa
     3061 tcatgcttgt caaggtcgtg gatataaaaa taacgccccg gttgatccat cggaatcgcg
     3121 ttgaaacgca gacgcatgaa tcgcccttgc gccgccgact gaaaaaaact gtagccaccg
     3181 gcattgttag taattatcgc accgtactcg gtcgaaccaa gataattgct ccaacttcgc
     3241 ggagtatccg gccgatcaat tacatattct ttattcttat cgtcaaaatg accaaactgc
     3301 atatttagtt actccactat ttaaaattaa accgtaccgc gtcgcgcctc gagagctgac
     3361 cggatctctg cgcatttctg ccgagtgagg tttaccctgg ttatgaaaaa aatcactaaa
     3421 attaatccca caacgggaat gcaagccagc atcgcccgaa tccagaaaat ggtctccggc
     3481 gcttgaactg caagactggg gtcgaaacga gcaaaactaa ggacgcttcc tgctaaaaat
     3541 ccccctaatg aattgcccaa cttaagaata tatgaggaac aaccggtgaa tgaaccctcc
     3601 cgccttttgc cggtgttaag ctcatcataa tctataatat cggcgccgat tgagccgtga
     3661 agcatccaga gaccagctga ggaaagcccc atcagaccag acgcaattgt ctgaagccaa
     3721 ggaacaagtg gcgtataaag aaaccaggaa ccgccatagc cgattattcc tataattgcc
     3781 gccactatta cggctttgcg cttttccaga taataggcga ctcggttcag gatcgggcct
     3841 cccagcaatc ccccaatcat aaaagcaatg cccatccaga aattccaatt atcaccgacg
     3901 atgttattac caccacatac ataatgcacg gtagtgtaat atccaagcgt tccaaccata
     3961 ctcgtgccca gagtaaaaga accgcccagc aagagcatca tccgaaacgg ctggcatttg
     4021 agagtttcat aaaaggaact tgccagtttg atacgttctt tagtcttgac aactactttc
     4081 tcgtaatagc gctcctttac cctgaaaaaa ataatgatgg cgaatattgc catcaagacg
     4141 ccgatgatag aggtatagac ctgcattccc cgcagagtat tttttcgacc ggtaccggga
     4201 ataagaaacc atgcaagatt agtaaaacgc agagcataga aattcccgac ctcgaaaatt
     4261 ttttgcatca cactacggta tgtcatcact gaagtacgtt cttcataatc aggagtcaat
     4321 tcgtttccga ggctgttata aggaacggta aataggctga tgatcggaat atagatgagg
     4381 ttcgacagta acatgtacca aaaaacgacc gataaaccga gaaacgtttg atcagcccag
     4441 gcaggcgaca ctaaaaatag cagtggcaat ccgaggccgc taataacgcc tgctcctaga
     4501 atgaacggtc gcctgcggcc gtgctttgaa cgaaaattgt ccgaaatcca gcccgcaatt
     4561 ggatcagtaa aggcatcgta tattcgaatt agcgtcagag ccaatccaac caacattgga
     4621 ttaacgccta aatacatatt gaaaaccgcg aacgcgacgc cgggatagag ccaaaatccc
     4681 cacatatcta atccagttcc taaaccataa caaattttgg ttaaaaccgg aaggcggtcg
     4741 cgtacctgcg tagttgaatt atttgccgtt atttcttgcg ccaatttcgt tcctttagta
     4801 aaagaatttt tctggcaaat tcagggttct atcatacttc tgaattattc ttgatataat
     4861 caatcaattc atcagtctga gcaaatgcca gagccgtata ggtatccgcc gcaccgtaat
     4921 aaatcgcaat ccgaccggtg gcagtgtcac acaagttggc acacgggaag acaacgttcg
     4981 gcacgaaacc ggtggtttcg tatggttttt ccggcgtcaa taaatagtct cgtgtccgat
     5041 ataaaacttt actaggttca tcaatatcca gaatcacagc cccaaaacta taaacaaaac
     5101 cgttacaagt cgttgatact ccatgataaa aaagaagcca gccttccgaa gtttcgattg
     5161 gaattggacc ggcaccaatt ttcgttccct gccaccaacc catcccgcct ttgcccatca
     5221 cgaatttgtg attaccccag taaatcaggt cgggactctc gctcaggaaa atatcgccga
     5281 atggtgtatg tccactgtca ctagggcggc tgagaagtag atatttccca ttcactttgc
     5341 gaggaaaaag caccccgtta cgattaaaag gcatcagcgg attcggcatc ctggtgaatg
     5401 ttttaaaatc ctgcgtttta cctaaaccga tcgaagcccc gtgcagatca tcacaccaga
     5461 cgatgtaata tatatcgtca attttcacta agcggggatc ataaccataa ctgagaggct
     5521 tgggattacc ttgctcatcc acccagttaa tcggatccgg cccgatttcc catcttaatc
     5581 cgtcgtgact cttaccggaa aaaagtgtcg ctcgcccatt tttctgatcg gctcgaaaaa
     5641 cacccacaaa ctcactttta tatgaaataa ctgcactatt aaaaatgcgg gcggctttcg
     5701 gaatgggatt ccacccgatt agcggattct tactaaatcg ccagactata tccgaacaac
     5761 cttccggcct ctcttcccag ggcaaatttg gaatcgagtt accgactatt cgatatttca
     5821 ttgtgaaatt ccttttcttt tataatgctt taagctggag ctattgtttc tccactttgc
     5881 gcccgacgct ctttcaaatc tgctccgata acagccatac gctgattgtt caaaggataa
     5941 aatgaaacaa ttatcaaagc caaaatacca aatgccgccg gaatcagact aaagagcaaa
     6001 agtaaccctc gcaaactttg cggcgtctga gcctgatttg gcataaatcc caaccgcgac
     6061 attaaattaa gagcaacaaa agccccaaac gcccagccga atttctgcga catcgtcgag
     6121 gcagaaaaca ccaatccggt cgcccgccta ccatttttcc actcagcata atcagctgag
     6181 tcggcataca tcgcccagat caaaacactc agaggcccac cggttaatga accgataatc
     6241 tggaataaga aaatcaaacc gatttgctcc ggcgataaaa aataatagat actggtactg
     6301 aaaatcgcaa taacgaaaat gatcataaaa gccggctttt tccccacttt tttggcaaac
     6361 cagctaacta acaaaacccc aataaatgac gaaatctgac cgacggtatt aaaaaccgaa
     6421 gtaagtgttt cgaaatccca ggttttattt ttaaaaaacg gcaaagaaac ctcacgtgtt
     6481 ccgacaatat atttaaaata atgaactgtc acactgcttc taatggcaac gaatagaata
     6541 aatgttatag tcgtaaaaag caatatcaac cagggcgaat ttgttactaa ttcatataaa
     6601 tcctttttta ttgaggattt ttgtgacttt ggcggatgaa cacgctcgcg cgtgcctttg
     6661 aaagcaatca ggaaaaagat aatagccgca attccataaa tcacaaatga taactgccaa
     6721 ccgcgcgctt cgttgccttt accaagtgct ttcaccatcg gcagaagcgt cgctgacaca
     6781 ataaccccag ccgtaaacgc aaacaaaaat ttgatcgatg atacggatgt gcgttcgtcc
     6841 gaatctggcg aaattactcc cagcaatgct gtatatggaa tattgatcgc cgtataaagc
     6901 atcataatca ggataaaagt cacataagca taaacgattt taccggtcat cccaaagtta
     6961 ggcacgctga atgtcagaac accagccacc gcaagcggta cacaaaacca caataaatac
     7021 ggtctgaatt tcccccagcg ggtgttggta cgatcagcta taattcccat caacggatcg
     7081 ttaacggcat cccagacccg actgacaaga aacatcgtag ccgctactcc ggctggaatc
     7141 aagaaaacat ccgtgtaaaa ataagtgaaa tatagcataa aagtctgcca gtaaagcacc
     7201 gacgccaaat caccaaaacc atatgcgatt ttttcccgta aggtaagata cttagccatt
     7261 gtcctctcct ttttttaaaa ttttaatccg taatatctta atttcactcc attataaaga
     7321 gtctattatc cccctaaaca gacagtttta tctttaaatt tttaacccat atctacgatt
     7381 acttcattgg tatttttcag cttagaacaa ggaattagat tattactcaa tgcttcacgg
     7441 tttaaagtta tccgccgcac acctttctgc actccggcag gatttcttac ttcaatattt
     7501 aaatttttcc cccggaatag acggcgcact gtgtagcttt tccagtcgga tggtatgcag
     7561 ggatcaatca gcaaaccgtc aaactgtggt cgaattccca gaatatattg cgagaccgcc
     7621 agaaaattcc aagccgccgt gccggtcagc caggaattct tagcttcccc cggtttatag
     7681 gcatccttac cagcaatcat ctgagcatag acatacggct cagtgcgatg taaatcgctg
     7741 atatcctcga gaaacgacgg acagatttta cgccaatatt caaatgcctg ctcaccgtta
     7801 cctaatttag tttcagcgat cataatccag ggattgttat gacaaaatat tccggcgttt
     7861 tccttataac caggaatgta agtcgaaatc tcacctaatt cgatgtaata tttcgtgtaa
     7921 gccggatttt gcaaaacaat tccaaattca caatcgaggt atttttttac ggaatcgagt
     7981 gctttttgca tccggccgtc ttccttgccg atctctgcca tacaacagaa tccctgcgat
     8041 tcgatgaaaa ttttgccttc ctcattttcc tgactgccga ctttccttcc aaaagcatca
     8101 taagcgcgca aaaaccattc accatcccag ccgaaccggt caatgctttt tttcatttca
     8161 tcaatatgct ggcgggcttc agtggcttca tccatttttc ccagaaattc acaaagttca
     8221 acgaactcct gaccatatag aacaaatgcc ccggcaatca taatagattc agcagtttta
     8281 ccgtcggttt ttaatggacc ggtttgaaaa gattgatccg gatcagtgtt aaaaatatta
     8341 agattaaggc aatcgttcca gtcggcgcgc ccgattagcg gaagtttatg aggtccaaga
     8401 ttatttacca catgataaaa agaacgcttg agatgttcga acaggctggc ttgattattc
     8461 ggatcattat cgaatggtac cgattcgtcc aatatcgcaa aatcgccggt ttctctcaaa
     8521 taagccacaa ctccacagat tagccagagc gggtcgtcat taaaattgtc gcccacttca
     8581 taattgcctc gcttgcttag tggctgatat tgatgataag caccgccgtt cggtaactga
     8641 gttgccgcaa tatcgagcaa acgttcccgc gctcgctccg gaatctgatg aacgaagccg
     8701 agcaagtcct gactggaatc acgaaatccc attcctcgtc caattccgga ctcgaaataa
     8761 gatgcgctac gcgacatatt gaacgtaacc atacattgat attgattcca gatattgaca
     8821 atccggtcaa tcttttcctc gccggtttgg acttgaaaaa ccgataataa accatcccag
     8881 aattcgcgta aatcgttcat agcctttgtc acactttccg gcgattcgaa gcgagattgc
     8941 atctgacgca ctttggtcaa attgattttg ccgtcggcag taaacttttc agtttcgggg
     9001 tattctacat atccgaggat aaaattgaaa cttttcgatt cgccaggatt gagcttaacc
     9061 tcaatttgat ggacggctac cggcgaccag ccaaaagcca ccgaattgcg atttttgccc
     9121 tcgaacacgg ctaccggctc gtgtggccca ttatgcaatc ccataaagga ttctttgtca
     9181 cagtcaaaac catcaatcgg ggcgttgacc gaataaaatg caaaatgatt gcgacgatct
     9241 ttataatcgg taaaatgcaa aattgtgcta ccgatcgctt ccgctctacc gatcgagaag
     9301 gtgcgctgaa aattagtcat atcttcccag gcattccaca agcaaaattc tacataggaa
     9361 aaaagtttga tttccttgac ggaattaccg gtatttttca aggtaacttg ctgaacctca
     9421 ccgtcgaaat tcataggcac aaaagccaga acttcagcta aaatatcatt gcgctgaccg
     9481 gaaatgcggg tataacctaa tccatggcga cattcataaa aatcgagcgc tttcttaacc
     9541 ggtttccagc cgggattcca aacatcgcca ttatcgttga tgtaaaagaa tttaccgttg
     9601 tcatccaaag tggaattatt ataacggtag cgcgttatgc gccggaggcg agcgtcgcga
     9661 tgaaaactat agccaccgcc ggtatgcgaa atgatggaaa aaaaatcctg actgccgaga
     9721 taattgatcc agggataagg ggtttccggc gtcgtgataa tatattccct gtcttgatcg
     9781 ttaaaatgac cgaatttcaa aactaatctc ctaaaatatc cttacacgta atatcccaat
     9841 aatcgaaacg tttatacttt tagaatttat aatagttcga tctctttgga aagtctaata
     9901 tcttgtgacg aagaacctat caatataaca aattttccct tttcagtcgt ccaattttta
     9961 attgcgggac tccagaaggc aaaatccttt tggcttaatt tcaaggttac agttttaatt
    10021 tcacccggct ctaacactat tttctgaaaa cctttcaatt ccttcgctgg gcgaggtaag
    10081 gatgattcga gatcactaac atatagttgg acaatttcgg cgccctggta cttacccaca
    10141 tttttaaccg aaaaggtgac tgaaacggta tcaacctcgg aaaatttccc cggtgtcact
    10201 tgcaaatcac tataagaaaa cgatgtaaac gacagtccgt gtccaaaggg ataaagcggt
    10261 tcgattttct gagattcata ccaccggtag ccgataaata ttccctcttt atatcgcaca
    10321 tcatataccg gatgcgctct ctcgccttcc ccattccaat cgaaataaag cgattcgcct
    10381 tccggaatat aaccgtaacc cggtgaatct ttgaactcct tttcaatggt aattggcaat
    10441 tttcccgaag gattaacctt gccgattaaa atttcggcca aggcttgatt cccaatttgc
    10501 ccgggatacc aggcatagat tattgctctg gctttctcat tccagccagt catacgtatc
    10561 ccacttcccg aagtcacaat aacgaccgtg ttcggattat tattgacaca tttaagaact
    10621 ttctgctctt gatcttccgg caattcaaat gggcgatccc aaccttcgct atcactagtt
    10681 cctacattgc acagcaccaa ttgggcagat ttgatctgct ttacggttgg atttttgaca
    10741 tattttatcc gatcaccaaa aactttctgc agttcagaaa tcatttgacg gttattgtat
    10801 ccctcaacat acgccgaacc accaccaacg gctaattttt tgacataatc gccggtaact
    10861 aagatatttt ttacctgttc ttttaccggc aaaatgttgt tttcattttt tagtaacact
    10921 attccttctc gggcggtttg caaggcaact ttttcatggg cttcatagaa actgtcttcc
    10981 accgcagttc gcgaccgacc atataatttc atagcaaaac aggtgcgcaa gatactttgc
    11041 gccattcgat ctatgtcgct aacttgcact ttattatcct caagaagctg atgtacattt
    11101 tccagcgcaa ttgtgaacgg catctccaga ttctgaccgg atttagccag tttctcaccg
    11161 ttataaactg accaccaatc ggtcattacc agccattgaa atccgagctg atttcttagt
    11221 agataattga tgacataagc gctttctcca caccattcac catttaacaa attataagaa
    11281 gtcataaccg cctttgcgcc agcttcgatt ccggctttga aggcgggcat ataaatttca
    11341 tgcaaagttc gttcatcaac aaccgaattg cttttcttcc gatagtattc ggtattattg
    11401 gctaagaaat gttttagggt ggctacggtt ccagtactct gaactccctg aacataattc
    11461 tctatcatcc gcgccgctaa gtagggatct tcgccaaaat actcgaaatt gcgaccgcat
    11521 tgggattggc gataaatatt catccctggt ccaagtaaaa tgccaatatt ggcggcttta
    11581 cattcttcac cgatcgttcg agcgtattga taagccaatt cacgattcca ggtggcggct
    11641 aatgaaatcg gacaaggaaa ggctgttgat ttttccggtt ggtagttcga gaggtcaatt
    11701 tcgttaaagc tctgacggat atggatgcct tgagtcgcat ccgacatata aatttccgaa
    11761 agattcaaac gcggtagcgc tcgaataaag aagtatttat cgccgccaat caaagcgatt
    11821 ttctcatcta aggtcatttg agccaagatc gattcggcta atttatctgc ttctgcatag
    11881 gctaaaggct gaaacgtttc catatctcga ttaaattcct gactgaataa acgattattg
    11941 gtggcgaaca ggtttagtaa aattagaaaa aataaaaaac cacctaagta ggttttgcgc
    12001 attataactc ctgtaattaa tttggttcaa tttattctct tagtctcatt ttataagtga
    12061 taattttcgg gaaatttgag tcgttccaat ttcggaatcg gctaaaaatc gtaatatata
    12121 taccccaccg gcaatttcgt caatcgcacc gttcgtttcc caggtggttt ggtaacgtcc
    12181 ggcgggaaac actttattta gcaaagtgca gatcaaatta ccgcgtaaat cgtaaacacc
    12241 aagattaacc ccggcgctaa ctggtaattc gaagcttatc tgggtagaag aattaaaggg
    12301 atttggataa ttttgagcta aggcaaatcc ggctggaata agtcgtgttt ttctaaccag
    12361 attgggttgt ttggtattac ctctgatgat ataatccctg attaataatc ctgattcggt
    12421 aatttcggat accgaccagc cgccgttagc ggacgcgcct ggttttaagg cggctgaggt
    12481 ttcattttta tcggcaattg accaattgca ccagctgatt tgattacttt ccataaaatt
    12541 catccagagg cgggtttcgg cggtatcaat cggaccatcg ccggtattca taacagtacc
    12601 aaattccgtc acaaaaagag cgatcccatt atttaatgct gttgatgcct tattgcgtaa
    12661 ggtttgttta tgatacggat aagccgcata aaaatgcaaa gcataggcga tattgttctg
    12721 cgcgagcgga ttctgcgagg caatatctac atcctgcgac cagtttggtg ttccgaccac
    12781 gattaaattg tcgggatcaa tcgcccttat tgaatcaaca acggcattca aataaggctt
    12841 taccacggtc ttccaattgt cctgttcagg ttcgttaaat acttcataaa tcaggttggg
    12901 ataggtaccg taatcagtag caatttcttg aaagaaagca atcgcctgcg cctgatgttc
    12961 atgagcatta tgatcatgcc aatccacaat gacataaatt cctaaatcaa tacaagcctc
    13021 gatgacggtc acgatttttg ccttttcgct tgcgggattg ttcaaataac cacccgattc
    13081 gatacccatt gccgcccgga caacggtgca tttccagtca tctcgaagcc atttaataca
    13141 attgtaattg tagtattttc ccatccattg gctccagaag agactcattc cgcgcaacac
    13201 tatcggatca ccattttgat caataatgcg gttaccgctg acagtaaggt ttccatgttc
    13261 ttctacgaag gttaaatcgg aaccaccgat ttgatcggat tcattattct gctcacattg
    13321 gataagaatt ggtaatataa ctattaacca aaacactgtg ataattttac acttatgacg
    13381 attatgtctc tgaactgaat tcatttttaa aaattccttt tagcgattga gtttttcacc
    13441 catcaatcat ccctatttat aaacaatcgg aaaattacct ttatattgac aacgatagtg
    13501 ctatttattg attatttcag attcagttgc tggcgaaccc aattcatccg cgcctggtaa
    13561 ttttctgccg gcaaaccgtg tccggaatag ggataaatga tgtattcttt tgggcattgt
    13621 aaattattat aagccgcaaa gttaatatgc ggcgggcagg tttcatccat cagacccact
    13681 agcataaaaa ccggtgcctt gactttcggc gctaagtttt taatatcaaa gtaactcaat
    13741 gttcggaaaa cttcttccca agttgtttcg ggatgattgt taacatagtc tttgaactcg
    13801 ttaccgggcc aaatggcaac tttaaaatag tcgggaaagt ctgataaaaa cggcacaccc
    13861 ggtacgcaaa ggcgaatacg atcatctaag gcggcggtcg caaacgaaag agcgccgccc
    13921 tggctctgtc cgtcaacgat gattcggctt gtatcaacct ctggtcgcga acacagaaaa
    13981 tctaccgccc gcacacaatc catataagcg ccgcggtaaa tatattcata tttattctcc
    14041 agatgatatt gcaaatagcc aggaaatcca ggatcgatct catcccgact attgccatgc
    14101 ccccgaatat tcaaaccgaa actgataaaa ccatcttcag taatcaggtt agtcggctgc
    14161 atattcgtac catagccttg aacttgaaga aaagccgggt attttccagg gtttttcgga
    14221 accgagtacc agccgcgaat taaaatattt cccagcgaac gcatttcaac caggtaataa
    14281 tcgcgctcag cggtgcataa ttcatcctgg cgaatcatct taaactgtgg gtcaaccgta
    14341 gccagttcgg ctttggcttg ctcccaaaaa gcatcaaaat ccggctgagc atcaacgggc
    14401 gaaatcacct tctccggatc aactgctatc gcaaatttat gcaatttatt ccagcctaaa
    14461 ctttcgaaac taaccgcccc gaggtaaaag ccgggttgca gtttctttaa gttgtaggaa
    14521 tattttttag cagaattatt tttcatcgta atctttaatt ctttatcata gattttatcc
    14581 ccgaaatcac tattgatcgc cagtctgaga ataccttcaa ttgactcgtt actttcatta
    14641 cgcataatga ccggaagtac aatttcggag caattcttaa tgacgtgatc cttctgagga
    14701 agggcaattt cgattttcag ctgatcggaa agaccaatca cattcagctc agcggctgag
    14761 tagtacaatc caccaccgcc acctgcgtca taaactcgaa ttgcaatgac attcggctga
    14821 tcccagcgaa ccagttcgac cgggataata tattcgcgat ccagaccata agcaccgata
    14881 taatttggtg ggaaaccacc catctgcccg attaattcac cattgaaata ggtagcatcg
    14941 gaatcgtcaa tacgccccag acgcagaata aatccgccat gtttaacggc tttctccttc
    15001 agcgttgatg gaataattac actgtatcta taccaggcaa atccatctaa attttcatag
    15061 ccttgcactt cccaggttct accggcaatg atagagttcc aggaagcgtc atcatattgc
    15121 ggatcagccc aggctaaatc gtcgccgggt tgaaatttcc aatttggccc tatcaaattc
    15181 tgagccgtta aaaccgtcgt aattgcaatg cagacaatta atgacgatgt gattgtttta
    15241 aaacgcattt tatccccctt tttaaacgtt aaattttatc gaaaacatat aaacatcatt
    15301 ttgtaaataa accgtttcaa atataattaa taaaggctat aaaatccgag actaaatttt
    15361 aaaactctct taattaattt ttataacgct tgttaccaga atggtaaaaa gctatctcaa
    15421 atctcaatct ggagcgaaac cggacaatga tctgaacccg ctacatcggc taaaatttcg
    15481 gcggcacgaa cattcgcaac gaatcgtttg tttacgaaaa aatagtcgag gcgccatcca
    15541 acattacgct ctctagcgtt ggtgaaagga ctccaccagg tataattacc cggttcatta
    15601 ttaaataatc gaaaagtatc ataataaccg tggctgagaa atttatcgag ccaagctctt
    15661 tcttcaggca aaaaaccgga aatattggaa ttcgccgcag gatgagctag gtctatttcg
    15721 gtatgagccg tgttgacatc gccacctatg atgatattcc gaccgctgac gtttacccga
    15781 tcaacataat cgagaaaagc gtcatagaat tcgagtttaa aacgcagtcg ctcctccgac
    15841 atttgcccat taggaaaata aacattaaat aaaacaaatt gatcataatc ggcaattagg
    15901 agacgcccct ccgtatcgaa tttctcgatt ccaaactttt gcgtaatgga aaccggttct
    15961 aatcgagaat acaaagcgac tccgctgtaa ccttttctaa ttgccgacga aaaataagaa
    16021 cgatagccac tgatttgggc taaatccggc ggtaattgcg attcttcagc ttttgtttct
    16081 tgaatcccta aaatatccgg ctgagtagct ttgacccaat ctaaaaaacc ttttttatat
    16141 actgcccgaa ttccattgac attccatgaa attagggtaa gaagcatatc ttagaaaacc
    16201 agggattgat ccaaacagaa ggcaagacct tttccgtgat taaagaatcc gcccgtctgc
    16261 gcaaaacccc attccaaacg atagtgctta aaagtcattc cgctaccgac gccccattta
    16321 attttatcgt tgcctccaac cccgattccg agccttatgg gcaaccagtt tgccggtaaa
    16381 aattcagccg cgaaagaaaa agccggggtt gttgtggatg ccatatcatt gctgaaagac
    16441 tggcgataat tgacaaacag ccgcagattt tgcaatactc tatattcaaa tcccattatc
    16501 atataagccg ggtaatttgt cctaataccg gaaactgtgt aggtagtgtc ttttcttaaa
    16561 gattcatcca ttattgaatc tatatcgcca aaatccgaac taggaatatc ggcataaaaa
    16621 gaatattcgg ctttcccggc ttctcctatt ccccaattta aaaaaccgac tacattgttc
    16681 aaggcaagat tggcagtcat tttttcatta ataacagacg ccgctcctat atcaatcgcc
    16741 agaccggcac cgcccagtcc gtattcgcct ttgagatcgc cttccaggat aattcggtct
    16801 ttataggtcg taacattcgc ggttagatcg tcgaatccgg aataacccgc tccagctaaa
    16861 agtttaacgg caataccgac ggaaaattta tcaacataat tactgagata aggcacatta
    16921 tcaatctgcc atccgtgagc tattcccaga gtaactaccg attgtaaatc cagatcagta
    16981 tcgcttaaat caataggctc ttcgaattca ttaccaaata acgcaagacg gaaaagcgac
    17041 ttgggtgcga caactttgcc ggttacttcg gcgcccagcg taaatgccca actatttacc
    17101 gaaaaattga gaaaacgcat ctgcaccaag ggattaaaat tcaaaccgcc atccggaaaa
    17161 tgacttagca attcttcctt agcatcatca tccaggtagt caccggtgaa aatatgatta
    17221 tttaataaat gtgttgaaat agtattattg ctaatctgta aagccggaaa tggcacgatc
    17281 gggataattc caaaattgaa ttgaaatttg ggattatcgt tcaatccgag attagccgga
    17341 ttccagccga tcacgtcacc accccgcgct tgtgtggcgt aagcatcggc aaaactgata
    17401 ctgcgcggat tcatatagag ctggctataa ccttgtgaaa ctataactaa taaagcccca
    17461 acaacgatat attttatctt tttcattaga ctaatcctcc tctttccgat cagcaaaatc
    17521 aatcaaacca ttaatcgttc cgtaaagcaa tatctttaat tcatcgccct tcataaaacg
    17581 ggaaggaatc ggattcccca tttcgtcagt tcgtccgacc agattgaatt gggttttgac
    17641 gtaaactgag tcctgaaaca aagctatttg cttttcacct aattctacaa cttctcgaaa
    17701 tgatgaatcg gggtgaacat taatgactgc cagagtatcc ggaccataag gtggagcaac
    17761 atcgaataat agtgtatcct gagcggctaa taagatcagt ttaccgccaa tctcgaatcc
    17821 attctctact tcggcataaa gaatggcgct ttcaatttct tctggaatgt cttcttccac
    17881 caattccgga tcaaattcca tcttggcatc ttcagttatt tccatttcaa acggtacaga
    17941 aatggatata ttgccgctaa catattgggc tgtatcaact tgaccagtac cgtagaccag
    18001 ggcataaccg gatgtaacga ttttcttcgg tttgatgttg attaattctt ctgcatcggg
    18061 aataacaacc cggggattct cggtaatgac ttgatggatt tcccgacgaa ctgattcacc
    18121 ttcatcatta aaggacgaaa taaataaatt gagttcgacc ggcacaccaa tatcggtatt
    18181 gaactctatt gccatttcga cattctttaa gttgattccg tcgaattctt ccggtaatgc
    18241 attaacttca ttttcggagg gttcaatatc cacttgaacc gtatcaatat acccgctgac
    18301 acttgcaaat tctacctcag tcagtagaac gtcaacattg atttcctgcc caaaagtgat
    18361 tgtcatttct cgatcggaag gaatactgac tcgcgaggtg tagtggattt cttggttggt
    18421 ggtcaaatcc gtaaacggca attgaatatt atattcatcc agcggaatgg tctcaatcac
    18481 cggctgggaa ctgtttggca ggtgaataat acgttctaat ggcgtaccat ctgtgcgatg
    18541 cacaatttca ttaattgtaa aatgaacatc ggcaaacact ccgatgttat taacaaaagt
    18601 caagatgaga ttaccattcg agatctgggc tgtctctaat ttagtcttat cttccagctc
    18661 aagtacctca ttagcaacga tcgcttcctg gtcaaaatat ccggtaacac tcgaaaaagt
    18721 catttcagat gtgttaatat caaatttgat tgaatcaatc aaactatatt cggctatttc
    18781 tcctgaggaa gtcgtaacca gacttttata ggtgatatac tgccgaacac tgtcagcggt
    18841 tacagattcg gacaatggtt ggattagaca attacttaag tcataattaa tcgtagtctg
    18901 tccgcctgga atatctgatt cgattgttac tggtgttccc gcagtagtta atatctctgg
    18961 aaatgtaaga acaaggtgtg gaattccagt tgtaccaatg ttaatgccgt tttcgattga
    19021 aaaggtcatt ccgccggagg caatttgcgc cgtactgatt cgggcatcgg aagcggtatt
    19081 gatttcaccg ctatctttga tgttttgcgg ctctacaatg cctttgatct ccgaaaaagt
    19141 tacatcagat tctgaatttt ctccatacat atctatgttg acattgactt gaccagatga
    19201 agaaatatca accatttcgg gatcggttga aatggttcga actatataag aatagttgac
    19261 ttcttgctcg tcaatatcca tttctaactt atggtcagtt agcaaatatt cctgaattac
    19321 aatctggcca gccggcattg gaataacttg ataaaagtca ctgccaccag aaacagtttt
    19381 taaagagggg atggtgagtt cgagattagc gctgaccggc aaatcattct gaatagatat
    19441 tttcaggtga ccatctttaa ttaccgctgt ttcaacttta ttgggctcgt cttccgcaaa
    19501 ttcaatcgtg ccagtagtgt caatggtctg ttctggaaca atcgcctccg ccgatgatac
    19561 agatatgtcc ttagcttgaa cttccaccac gaatgaagaa ttcaaagtcg cagagttttt
    19621 agtgactatt tgcgaacctg agccacaaat gactccagaa acctccacga tgattgtccc
    19681 gggcaaagtt ttattggtta gatttattgt ttttttactg gattgcccgg atgccaaacc
    19741 ctcagtccag atggctttag ccgaatctcc gtccgttccg acaattggat ttccagaacc
    19801 atccagtaag cggacataaa ccggtgcacc aagttcaact accagattat tgcggatatt
    19861 aatttcgagg aatcctccaa taaaattagc attattaaaa ttattaaaaa caatttgtcg
    19921 atttatcgtc ggaaaaggtg tacttatggc gatggtcttg ctggctccgt ccgctatgcc
    19981 ggtcatatcc actatttccg aaaataaaat cggagccgtg ctttcgacat cggtatcagc
    20041 cagatgaatg tcaccgatta ggttgctatt ctgttcagaa accggctcaa ctccgacttc
    20101 atcaaaaccc gaagagtatt ttttatcact tgcggcaata ttgacttcat ctacaccttg
    20161 ctgaatggat tgagttatat catcaatatt taatttatta ccgacccgaa cttcttcaat
    20221 tgggatttga tcttcatagg caaaaattga atcgccagcg gtaccataag gaagcttgac
    20281 tatcagcgaa tcttctaata aatcattgac ggtaacagtc tcatttaata gcgggacgtt
    20341 cagatttacc gaccaggtgg gataatccga ttcactgggg attttaagat cgcaaaatga
    20401 aaatagaacc gccactccgg cgatgatgat gattttaaac ttttgcatnn nnnnnnnnnn
    20461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnat ctggcaacct gttccccgaa tccaccgaca
    20581 atgctgttct cttcaatcgt gataataatg cgatgcttac tggcaatctc cgacaacatt
    20641 tgagtatcaa gtggtttcac aaaacgagcg tggacaatag tcggaaatat attttttggt
    20701 ttaagtaggc aggccgcctc tatcgcgtta ttggtcatta cgccgaccga caagatggcg
    20761 acgtcttgac cattttgaat tatctcccac tttccaattt ggtagggcac ggctggtgct
    20821 tgatcgtcaa attcgacata agaatctttt ggatagcgaa tggcaaaggg cgcatcgttc
    20881 tgctcaagag ctgtgaacat taaactgcgc aattcagtgc cattccgtgg tgcggctacg
    20941 atcatattgg gcactgtgca taaatagctc aagtcaaatg cgccatgatg ggttggaccg
    21001 tcttcgccga cgattcctcc tcgatccagg acaaatacta ccggtaaatt ttgcaaagcc
    21061 acatcatgga taatattgtc gtaagcgcgt tgtaaaaagg tagaatagat tgccactacc
    21121 ggtcggaatc cacaagccgc taaaccggcg gcaaaggtta ctgcatgccc ttccgcgatc
    21181 ccgacatcga agtaacgcat tggaaagcgt cgtgcaaatt cgactaatcc tgtgccttcc
    21241 gccattgcgg cagtgataaa gatagacttg ggatattttt caccaatttc acaggcgatt
    21301 ttactgaaag catttaaaaa gggcggagcg gttggtgttg agacacaagc attaacatct
    21361 ttccgcggac caattccgtg atatttaatc ggatcaattt cagcgctggc cagccccttc
    21421 cctttttgag taataatgtg tagaatgaca gggtatttca agtctttgat attattcaaa
    21481 gtattaacga gttgctgaat attatgaccg tcaaccggcc cgtaatagcg aaatccgaat
    21541 tcctcaaaga ttatcccggg cgcaagaaaa tttttgaatg actccaataa tttccgccct
    21601 gccttccgaa aaaaattggt gccgcggggt aatagcgcca gtgaattcca gacttggttt
    21661 ttaaaattca ggtaactagg actggttaca atgcgggtaa aataattaga cattgccccg
    21721 acattacgtg aaatcgacat ctcattatca ttcagaatta ctaagaattg gccgttaata
    21781 ttataagcgt tattcagacc ttcataagcc aatccaccgg taaannnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21901 nnnnnnnnnn nnnnnnnnnn nnnnattgta agtgattgag aaaatattga tgaatacgaa
    21961 aatcgtcagt caattccgga tgaaaagttg ccatgagatt attgcctgac cgggcgagaa
    22021 ccacatcgcc atttaaatgg ccaagaattt caacagaaga atcgagatga tcgagtttcg
    22081 gagcgcgaat gaaaatgcct ttgaagggtt tgtcggaaaa cgtaagatca acattttcaa
    22141 caaatgattc tacctgccgt ccgtaggcat ttcgcttaac tttaaccgaa ctacgtccta
    22201 atgatttgac caagggatcc gtgccgtttg ttgatagcaa aattgctccg gcgcaggtac
    22261 caaaaatcat cccgggaaat ttctcaagac tttcgcgcaa atccatttcg tcaattagcc
    22321 ttgacatcgt ggttgattcg ccaccgggaa ttatcaaacc gtcacatttt tcgatttgtt
    22381 ctttatagcg aataacgatg gcttcggctc cgagggaatt gataattttg aggtgtttct
    22441 gaaaagcgcc ctgaagtcct aatacgccaa ctcttctggc tttattgttc ataagatagt
    22501 gagattcaat atactatttc tatttttacc aaccgcgttt tgcgagtcgt tcattttccg
    22561 gaatctcggc gagatcgagc ccacgcatag cttctcccaa tccggccgaa acttcagcga
    22621 ttttttcggg attattataa taagttgtcg ctataacgat agctcgagcc cgagcggctg
    22681 ggtctgcgga tttgaaaatt ccagaaccca cgaaaacact ttccgctccc aattgcatca
    22741 ttaaagaagc atctgctggt gtagcgattc ctccagccgc aaaattcggc actggtaatt
    22801 tgccggtttt agcaatctgc ctaaccaatt caaagggagc gcccagacgt ttggcttccg
    22861 ccattaattc atcatccgac aaagtcgtca gtttacgaat ttcggattgc acccgacgca
    22921 tatggcgcac cgcctcgacg atattgccac taccggcttc gcctttggtg cgaatcaaag
    22981 ccgctccttc gccaatgcgg cgcaatgctt cacctagatc gcgacaaccg caaacgaacg
    23041 gcaccttgaa atcgtgcttc caaatgtggt tttcttcgtc agccggagtc aaaacttcac
    23101 tctcatcaat aaaatcaatc cccagcgctt ccagtacctg cgcttccgca aaatggccaa
    23161 tgcgacattt cgccatcacc gggattgaga ctgtatcctg aatcgcttta attactttgg
    23221 gattcgacat acgggcaatt ccccccgccg ctcgaatatc ggctggaatc ctttcgagcg
    23281 ccatcacagc gacggctcca gcctcttcgg caatcttggc ttgctcgggc gttgttacat
    23341 ccattatgac gccgcctttt agcatttcag ctaagccaac ttttacttca aaagaacctt
    23401 tatccatttt gaataattac tccttttcat acgattaaat ttaagacatt tcagtcaaaa
    23461 ttggttattt ttttaaccac ctcttccacc acttcaacag gcgtcgaggc tccagtcgcc
    23521 acgccgactg atgtcttatt aataaaccag tccggattga tttgctcggc attttcaatg
    23581 tggtgagtat tggtattcaa ctttttggca atttctgcca gcctccgtgt gttggcacta
    23641 ttttttgaac ccacaaccag cataacgtca ttggctaaag cgagttcccg aatttgttcc
    23701 tgacgttgac gagttggacg gcagatagtg ttcataaagc gtaggtcgtc ggttttagcc
    23761 gccagaatcg cgacgattgc cgatacattt tcaatcatct gagtggattg cgctacaacc
    23821 ccaatttttt tcatctttgg caaacgttcg gcctcctccg gactcgaaac gatgatagga
    23881 tcgttgattt gactggcaat gccgctgact tcttcgtgat ttttatctcc gataattatc
    23941 acctgacgcc cttctttggc taacatttgg acttcgttgt gaattttacg aactagagga
    24001 caggtcgcat caataattct caagcctcgt tgatgcgctt catcccagat ggatcgaggc
    24061 gtaccgtgcg agcgaaataa aacgggtacg gttggttcga tctggtcaag gttttgtact
    24121 acttcgacac ccaaatcggt taattcacga atcacttttt cattgtgaac aatgtcacca
    24181 agcatcgcca cccgaccaaa tttttgagcc gcggtttgcg ccttctttac tgcgttttgc
    24241 actccggcac aaaaaccgac cgtagccgag atgttgactt ttaaagccat tattccttga
    24301 tcttcgcgtt cgtaatgttt tgctgggctt ggtattttcc ttttttatcg gcgtacgaaa
    24361 tccgacaagc ctcgtcgctt tccaaaaaaa taacctgcgc gacaccttcg ttggcataga
    24421 ttttcaccgg taccggtgcg agattggcaa tagaaatggt aacaaaaccc tcccattccg
    24481 gctcaagcgg ggttacatta acgaaaatcc cgcacctagc ataagttgac ttgccgctcg
    24541 taacggcaat gacattgcgc ggtatcctaa aatattccag actacgtccg agtacaaaac
    24601 tattcgacgg aatcgtgcaa actgcacctt tgtaatcaat aaataaatcc atcgttattc
    24661 gtttgggatc cagaactgta tcctttcgac cgtgaaaaat acgaaattca tccgccactc
    24721 gcatatcata gccgtaggaa gatacaccga aggaaattac tcctgaacta atctgacttt
    24781 tttcgaatgg ctcgatcatt ttatgtttca aagccatttc ctcgatccag cgatcggatt
    24841 tgacgctcat tttatccttc atccagaaaa actttgcgca aagccggtaa aactgtttct
    24901 ttggcttctt ccaatccgac atccatcacc aaacccgccg catacggatg cccaccaccg
    24961 ccaaattggc gcgcaatatt attaatagga taacgccctt tggaacggaa attcatccgc
    25021 gtgcggttat cacgctcttc cagaaacata gcggcaattt cgacaccccg gattgagcgg
    25081 acaacatccg taaaaccatc aacttcatct aaataggctc cggcggcggc tacctgagcc
    25141 cgcgttacgc aaaaccatgc caaccgattt tcaaattcat attgaacatt ctgcaatacc
    25201 atcccgagca atttcatcct ttccggacga tagttctcgt aaacatagtg ataaatcaat
    25261 cccggttgaa cgttataccg caaaagttca gccgctaata gcatcgtttc agcagtagta
    25321 ttttcaaatc gaaatgaacc agtatcggtc attaagccga cgtacaaagc ctcagccact
    25381 ttttgatcta ttgcttgcgg cgctatgatt tttaataatt caaaaaccag tgcgactgtt
    25441 gccggggctt tatcatcaat gacgctaata ttttccggca ggcagttatt aaacggatga
    25501 tgatcaatgc aaatagtcgg aatattaagc gatttcagaa ccggaccgag cgccccgagt
    25561 cgttccgtat ggctggcatc cagaatcatt aacaaatcga aatcagttag ttcaacttga
    25621 tctaactcgt tggggaaagt atgaaagatt tcaccctcat tcagaaatga atactccggc
    25681 ggcaaaggcg atgaattcaa gataaatggc gacttaccca gacgcttgag gcaatggtaa
    25741 agcgccgctt ccgcgcccag cccgtcgccg tctggattgt ggtgagtggt gagcaggatt
    25801 ttatccgact ccttgatttt ctctacgatt ttttgccagt tatatttctc catnnnnnnn
    25861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaaaaag gagaatcaac tatgacgaaa
    25981 cgatctcaaa tttacaaatg tgaaatctgc ggcaatatag tcgaggtttt acacgaaggc
    26041 gcgggcgaat tggtttgctg tggcaagccg atgaaactta tcgtagaaaa tacgactgac
    26101 gccgcccgag aaaaacatgt cccggtaatt gaaaaaatcg ccgacggcta caaggtcagt
    26161 gtcggtagcg ttctccaccc aatgattgac gttcattata ttgagtggat tgaattggat
    26221 accgacggtc aggtttttcg ccaatatctg aaaccgggtg atacaccggt ggctattttc
    26281 aaagtatcag ctgaaaaatt aaccgcacgt gagtattgca atattcacgg cttatggaaa
    26341 ggaacattat aatgcttagt cccaaattat taaccgcttt caacgatcaa atcaaacacg
    26401 aactggaatc cgcctatctt tatgcggcga tgaatgcctg gttcaaatcc gaaaacttcg
    26461 acggaatggc gcattggatg gaagtccaga ccaaggagga aataaaccat gccaggaaat
    26521 tttacgacca tattttcgag cgcggtgatg tcgtcgaact ccagccaata aatatattag
    26581 ccaagaaatg ggcttcgccg ctcgacgctt tcaaagccgc ttatgagcac gaacaatttg
    26641 ttaccggcaa aatcaatgat ctgatgacga tggctattgc cgataaagat tatgccgcac
    26701 aggaattttt aaattggttc gtcgctgaac aggtcgaaga agaagattca actttgaaaa
    26761 tagttcaact tttaaagaga atcggcgatc acggtcaggc tttgattatg gttgatcgag
    26821 aacttggtaa gcgcgaataa taggtaagag ccaattatga attttaacat agacgaagtt
    26881 ttcgaaattg ccgagcagat agaagtcaat ggcagtcgtt tttatcgtca agccgctgaa
    26941 ctcagccagc tggctgaaca taaaaatatg ttattaaagt tggctgagat ggaagatcag
    27001 caccaaaaaa ccttcgctgg catgcgtgaa aaaataaaac aaaccaagca aaatttgtct
    27061 tatgatccca atcaggaagc cgctctcttt ttacaagcct ttgcttctgg atacgtgttt
    27121 gatccaacgg ctgattcatc ggcgttaatc agagaacaaa aatcaattgc ggatattttg
    27181 cacttagcca ttggattgga aaaggattca atcgttttct atctcggcat cgaaaaaatc
    27241 ctgctcaacg aagacgatat cgttcacgta cgtgctatta ttaaagaaga gatgggacat
    27301 attactctgc ttgcccgaaa attagacgaa ctgaattaag ctaaacggca atttgttttg
    27361 atttaatacc cttacaggta tgattacttg tgagggtatt tttttataat attcttgatg
    27421 aaattaatgc aggctgttta aattgacgat aatgattaac actaaaataa ttaaaggaat
    27481 aaaaaatggc aattcgtatt tttgtaacgg gcgggacttt tgacaaagaa tataatgaac
    27541 tcaccggaca actctatttc aaagacaccc acttaccgga aatgctaatc ctcgggcgct
    27601 cgcgggtaga agtcgaaatc cgcactttga tgatgattga tagtctggaa atgaccgatg
    27661 atgaccggct attgattgct caccattgtc agaaagccgc tgaagatcgg attgtcatta
    27721 ctcatggcac ggatacaatg gtagaaaccg cccgtctcat tgcccaaaat gtcagcggca
    27781 aaactatcgt cttgaccggc gcgatgattc cttataaatt tggtagttcg gatggactct
    27841 tcaatctcgg tagtgcttta gcttttgttc agacactttc tcccggcgtt tacattgcga
    27901 tgaatggtcg cttttttagt tggaataacg ttcgtaagaa taaaaagac
//
LOCUS       DAOOMU010000018        26446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1679_length_26446_cov_12.182373, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000018 DAOOMU010000000
VERSION     DAOOMU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 26446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26446
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1679_length_26446_cov_12.182373"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLI55_02990"
     CDS             <1..363
                     /locus_tag="PLI55_02990"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPA99834.1"
                     /translation="GVAFVAEGDGFQSRLRILVGMDPEMTKIVQMKILSQAETPGLGT
                     KIENDPTNKTDAFWFLKQFADLPAVSPISVVKGQAADKSKSQIQAITGATISSRAVVD
                     IINAAIEKNRALYLEKVK"
     gene            365..979
                     /gene="rsxE"
                     /locus_tag="PLI55_02995"
     CDS             365..979
                     /gene="rsxE"
                     /locus_tag="PLI55_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HPA99835.1"
                     /translation="MAKSKISVTQEFLKGLWNENPILRMVLGTCPTLAVTTSAINGVT
                     MGLAVIFTLVMSSMMISLLRKLIPNEVRIATFIIIIATFVTVADRVLAAFYPEMSKAL
                     GPFVPLIVVNCIVFARQEAFASKNPVGRSVIDALGMSVGFTLVLLIMGSFRELIGNGT
                     IFSHQIMPLSFQPWMAIALQPGAFLTLGFMIGFANWLNTKGKKK"
     gene            976..1548
                     /gene="rsxA"
                     /locus_tag="PLI55_03000"
     CDS             976..1548
                     /gene="rsxA"
                     /locus_tag="PLI55_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HPA99836.1"
                     /translation="MNLILIFISAAVINNYVLVSFLGICPFIGVSKKLGSAVSMGMAT
                     TFVMTITAAVTWLLNKYILVGLHLEFLQIVSFILVIASLVQLVEMFIKKVSRPLYDAL
                     GIFLPLITTNCAILGLALFLVMKQYNFVESIVFGFASGAGFTLALVIMAGIREELELA
                     DVPEPFKGAPITLIVAGCLALAFMGFSGLI"
     gene            1567..2385
                     /locus_tag="PLI55_03005"
     CDS             1567..2385
                     /locus_tag="PLI55_03005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPA99837.1"
                     /translation="MNSTTFLYSLLSMGGIGLFFSILIAVANKKLHVEEDPRISLIAE
                     CLPGANCGSCGYAGCNNFAEALAGGKSQISNCPVCSPSAREEIARILGVSVEIGERQV
                     AVVFCQGGDDVATKKAEYRGIKTCSAANLVSGGERACSYGCIGLGDCVTVCPFDAIHM
                     NENNLPVVDRAKCTGCGNCVTACPRGIIELHPISHHVFVLCRNRDIGRIARTVCKRAC
                     TGCLLCEKAVNGQGFKVVDNLAVVDHDVYTKELVLPTEKCPTKAIVILGTKDEQ"
     gene            2375..2848
                     /locus_tag="PLI55_03010"
     CDS             2375..2848
                     /locus_tag="PLI55_03010"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HPA99838.1"
                     /translation="MNNNDQYFKTDDETATVIDADQHWVTVELVEKDTCHSCGAKMIC
                     RPNSTGKRILKIPNTINAKVGDTVLIEQIGANQLKLTIVQYGLPLFGFLFGVLIANRF
                     IKHSIFGIPADIVQMFCGIIVVILTGFIIYFWSKRKAKSGFNVFRLRQTLNGKFE"
     gene            2865..>3097
                     /locus_tag="PLI55_03015"
     CDS             2865..>3097
                     /locus_tag="PLI55_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99839.1"
                     /translation="MLNFKIPIPYFLFCVFLLTSCQPKSKLVKAQSTRFAMGTIVEIT
                     VLDTTEAEANNALEAGFAEINRISAQFWEGNPEG"
     assembly_gap    3098..3197
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3198..3722
                     /locus_tag="PLI55_03020"
     CDS             <3198..3722
                     /locus_tag="PLI55_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPA99840.1"
                     /translation="QLGAKNHQTKIGMGAVAKGYGIDQAVAVIAKHNVAGALVNAGGD
                     LRALRRVDGKKWRIGIQDPRQADNILLVIEFDSGAVATSGDYEKFFMYEGKRIHHILN
                     PRTGLPADSCQSTTVIAATAEQADAMATGLFVLGALAGQKALENFPNCEVLWICADGQ
                     KVQSAGFEKYLAQH"
     gene            3941..5314
                     /locus_tag="PLI55_03025"
     CDS             3941..5314
                     /locus_tag="PLI55_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA99841.1"
                     /translation="MEDNKPVQNPLREIVDPFVNLIKAPRALWGINLSYLLEGITYFG
                     ILSLLAMYFNDLGLNDIQAGQMVGIQTAGITLAMLFLGATVDWIGVRKALLISLSTML
                     VGRVLLALAPQIGAPGLWQKAHIVAMLGILGVVLGYGIYQPACYAAVKQFTDKKTSAM
                     GYAMLYAIMNLGGFLPGLISPPIRKNHGIVGVYWVYVALTVVGILVVAFILSKKTVEM
                     AIANAREGQDEPVSDPETTETQSTKEKIVYYLKNFPIKDGRFMFFIFILIPVQTLFAH
                     NWLTLPMYFERAFSGVVSNNFEFFVNFNPILIFILAPMVAALTAQKDTYKMMILGTTI
                     MALPTFLLAIGPNIYSVFGYLIIMTIGEAIWQPRFLQWVAEIAPKNMTGIYMGIGQFP
                     WFLTKVITSLYSGWFLTKYCPAGVTPEQTNTETMWLIYGFIAIISPIALLIARKWMGK
                     GLEKQKA"
     gene            5315..5686
                     /locus_tag="PLI55_03030"
     CDS             5315..5686
                     /locus_tag="PLI55_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPA99842.1"
                     /translation="MLIKPKILIVDDEAVIREIYGDFLEQNGFEVATALNVETALESI
                     KAKKPDLILLDIVMPFRNGFDLYDQVIVDYPGIKFVFMTGYDDDIKITDRLSKSGKKW
                     FTKPIKLEEMLEIIRNELSAS"
     assembly_gap    5853..5952
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5953..6320)
                     /locus_tag="PLI55_03035"
     CDS             complement(<5953..6320)
                     /locus_tag="PLI55_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPA99843.1"
                     /translation="MRKILAVDDDPNIIELITSYFSSRGFEVYATTEGAQVLDLAISK
                     EPDLIILDINLPDIDGLSVLKTLKNRPITAFIPVIILTAKTSADFQVDGLVTGADDYV
                     TKPFDLNVLYARVLNSLRRSL"
     gene            7199..9046
                     /locus_tag="PLI55_03040"
     CDS             7199..9046
                     /locus_tag="PLI55_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPA99844.1"
                     /translation="MKNATENQVPKGKFSLARGLLVVGIVAGICLSDNLSDRTYWTYD
                     AIWYQIFPERFRNGDPTNDPTIETLEGTWPYEPQTQWSIMPWTADWYKLQPWEKSNQR
                     DFYYNAQLRRYGGDIEGIIDKLDYLVDLGINAIYLNPVFESASSHKYGATMYHHIDNN
                     FGPDPAGDIAIWQTENPNDPLTWQWTSADKLFLKLIQEVHTRQMKIIIDGVFNHVGIP
                     FWAFQDVKKNGEASPYADWFVIKSFDDPSTIEDEFEYQGWNGIPDLPEIWEDENGPVQ
                     GFRAHIKAVVERWGDPNGDGDPSDGIDGWRLDVAEKVSKNFWRDFRKWVKSINPEAYL
                     TGEIWWEDFMNNKMFDASPWLQGDIFDGVMNYRFGDAMLKAFIDREMRIKPSELDQLL
                     GDIREKYPLPAQYQLQNIIGSHDNERFASMLLNPDRWIDHGGNLVYNKDFKINRPSDE
                     LRQIQKTILVFQFTYIGAPYIYYGDEVGMWGADDPDNRKPMVWDDMQYEPETTHPFGI
                     KRPVDSVKVDTDLFNFYKSVINLRKNHECLRRGRYKTILTDDKKGIFGFERKYGTETI
                     YIFANLNDKPIKMSEINFDVTKCEKIFTVNTDTNYLGSKGIVVYKIIEG"
     gene            9092..11890
                     /locus_tag="PLI55_03045"
     CDS             9092..11890
                     /locus_tag="PLI55_03045"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPA99845.1"
                     /translation="MRYKDWLITVVLILTAGYLFGGNTGKITGIVTDSQTGLPLIGCN
                     IMLEDTYLGAASDTDGRYMILNVPPGEYRLKAQMIGYQPKVITGVIVSADLTTTINIG
                     LSTQVLEGEEVIVTAERKMIVKDMTATASNVKAEELAALPVTEVSDVLEMQAGYVDGS
                     LRGGRRGEVAYLIDGIPVTDSYDRSAVIDVNKNMIQELQVISGAFNAEYGKVMSGIVN
                     ITTKQGSNKHGASLEMYIGDYLSTHDEIFDNIGSINPLNIHNAEISFFGPLIKDKLFY
                     NFNTRYIYFGGWLHGQNRYKPTSILAVSPDGSHFLLDSTTMLGDQKWVNMNWNEKLYF
                     QGQLIYRLSNRSSIYYNFFYDSKDYQDYDRMFKYNPAGMLKKNLTAETHLLKFQQQLS
                     SKTFFTLAGSYYYRKYTGRLSADTSYNDSTTYVHPAYLYQYPYQFYFGGTQNDHEYRD
                     SRTYLAKWDLTSQINGQHQIKGGLEFQQHDISLRTFNIRPATGETYIDFASGLNIPST
                     WNPYIHPTILDNSTIYASYYRHKPYEFAGYLQDKMEFSELIINIGVRFDYFEPDGVVL
                     ADPSDPEIYEPIKPENRYHDLNGNGVQDPGEPEVTLEERRQYWYKKATPKYQFSPRIG
                     LSFPISDRGVFHFSYGHFFQIPNFNYLYMNPDFDLGSGTGNQGVVGNADLKPEQTIVG
                     EIGVQQQLSDNFSVDITAYLKDIRDLTGTRAEEIIVYGGAASYSRLVNSDFGVVKGIT
                     IALNQQNPSGLYTSFDYTLQIAKGTASDPEAYRNAIAGGNQPEIQLNPLDWDQRHTIN
                     GTVGYNHKNYGINFIGRWGSGLPYTPQSSEDITSLLTNAGTKPPTLTIDARSYYRFTL
                     SGRYSVELFIRIQNLFDKLNEVNVYDETGRAGFTTDEARVEALNIKTPINSVHEWFND
                     PTYYSEPRRVEIGMRISI"
     gene            11887..15798
                     /locus_tag="PLI55_03050"
     CDS             11887..15798
                     /locus_tag="PLI55_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99846.1"
                     /translation="MKHSIKYLTIEILSILLMTIVTSQIIFANQSGKAYRRSSIHNGN
                     LVRTVFGNWGVIGQPREKGPRGAWLFDTNGYIGDVSPMVGAEVNYHSEELDSTIIFHS
                     VVVCPVDRPATAREESPTGDQWTFEPVSGYFNESSQGIAMSTDRYSWPPYWPDKMDDP
                     EDPGWPEQWNGFFGKGVTNADQESYFIMDDNNDVEFNSGHNLKGVYFKPDNRDANRNG
                     LGLEVKVRGMQWAQFLAQDVIFWLYEITNTSTTDYDKVVFGMIAGTYNGVTGTDDSPQ
                     EYDDDYSFFDVERDLTYTGDYPNNNSRNPKWQGPVGLVGYAFLESPGNPYDGIDNDGD
                     NKPEEHPGKNLIFTAPLFTESDFDSVVYNKGDQIVLIDKDYKRTFHTITSDTETVVTK
                     GMQITIIAGQTKLVEGNVLANGSINPNVYDGIDNDLDGLIDENYLLHYRQRRVDQNGV
                     VLFDTLNPVAYKDYRRGLGIDDPLIDEARNDGLDNDGDWNPETDDVGSDGKPGTNDYG
                     ENDGKPTLGEPNFDKTDVDESDQIGLTSFNYFVPSNAYPMADDELLWEWLKPGYFDVP
                     SSIQNGKPVAGEDGDFIYGSGYFPLRAGDTQRFSLALVYGYDLTDLLKNRETVQDIYN
                     SDYRFPQPPDKPTVTAVAGDGKVTLYWDRKAEESIDPVTKEKDFEGYKIYKATDPDFN
                     DVFVITDANGVLTGYKPLAQFDKVNGIKGIFEASSDLYQSAGGYSYYLGSDNGLVHSY
                     VDEDVQNGRIYYYAVVAYDHGDASNDIFPSENTKFISVQSDGTVITDINTVQVRPTAK
                     VAGYDEQNSELALKHLEGPATGVIQYKIIDETLLNGHTYKVNFTDTRTDGIDNDDDWN
                     YLTCDVGSDGKPGTNDYGEGDGIPTSGEPNFEWNDEDEFAQITSTYSVLDKTGVEEFI
                     NLDTSYVWLTHQNIQYDDILIASESDPNNYLSLSKFEIDTLRGKIKIKADSNLKSGSY
                     RIFYKYYPVFKSPNIQYSPYMKETKDSDIFDGVELVFSNPWDIKMIKSKSGWNSTDRI
                     IDFTFRIDQALLEGDTIVGVQYPGIYELRVGDSSEVLYETPKEFLKSIYPSFSDAIIR
                     TLVKKTNFYLFNVLDSVKIPFLYNGGIKNSYGIYEITPGGLIESYIPKPDSTYQYSWV
                     INFPPVSEKVTPPRFLKGDILHIETEIPFRNGDIYEFTTEIPNVDDEVAKSSLDDIQV
                     VPNPYVVANTMESPLPPAITSGRGERRIEFRKLPNDAKVHIFTSAGAHVITLNHNGNI
                     HNGTLAWNVKSKENLDVAFGVYYYIIESSVGKKSGKLAVIK"
     gene            15809..16822
                     /locus_tag="PLI55_03055"
     CDS             15809..16822
                     /locus_tag="PLI55_03055"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPA99847.1"
                     /translation="MNRKLPTIISTVLISTIFLNAQNKEGTAAGQFLQIPVGGKAIAM
                     GGAFTALADDASAIYYNPGAISRISKNKLYVSQTNWLVGGKHQFIAAQVMATSNDIIG
                     IGVDYLDYGEREKITTVAEPDGTGEYWEASDLAFSLGYARNLTDRFSIGGSVKFIQQN
                     IWHVSATGFAADLGLLFKTQFNGLQIGASMRNFGSELQLTGRDLITQVDLDEEASGNN
                     ETIVAYLKTDKWPIPLTFTIGVAMPVINSNQFVLTLAGDVVRPTDNSQTLNCGAEAII
                     MKMISLRAGYQALFREKTQQGLTAGIGLKLPIQYADFYFDYSYQEFGFFGDLSTMSIS
                     IGF"
     gene            16845..18896
                     /locus_tag="PLI55_03060"
     CDS             16845..18896
                     /locus_tag="PLI55_03060"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPA99848.1"
                     /translation="MKKATVLMLMLTLIAVSGLADVTVNYIVNTSTLQGVTDSTHQVQ
                     ICGAEVGPEGVDKWYNLFLTWGDDSPLAANIGGDYWKLTIAYPDSMIGWRMAYKVRYK
                     ANDDDAFTWENVTGGGNRMWVLPANDTTLAVAYVNNDFNPPFTPSDSVDILVRVNMEN
                     YEDSFSLLSVVGHWPFCDPEWTPGALPLTQEGTSDYWSRTIRIPQAVMDTVDRVQPLG
                     AEHPGVLMYRFAIGNDWNNSENLNGKYWPGNENRIIALHPGLEDTTLAWKYWNDAAPA
                     GFEAEDTVDITFTADLTNAIANKGFAVGDTILVRMGYFNSLSSTMDANLLRISGTYLY
                     STTFTDVPVVLNEPLYYQYYLVKDAVEQREVYFNFDYEGEDVTRAERRAYVITGDAEI
                     NDIEDSETDPRRMPKFRNNQKLSQAVTVTIECDLRPAYYQVWAGSTLDDIQGNIDITP
                     AMLEANPDTLAKLGLCINGPMSNNGEGTWATWGGTLASDATRRMYDDGTNGDETAGDT
                     IYTIVYNFTTEKTIGQEFKFGIGGGDNESSYGLNHIENIDDTNPTFTLEVQFGSINPN
                     FYSAWDFNARAPKVGIKNQNVSVAKEFSLSQNFPNPFNPTTEIRFTISEKSDVHLTIY
                     NLLGKVVNKVTYNNLDIGNYSYVWNSKDLNGNSVASGIYFYELRAGSKYHAIKKMVLM
                     K"
     gene            18971..20803
                     /locus_tag="PLI55_03065"
     CDS             18971..20803
                     /locus_tag="PLI55_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPA99849.1"
                     /translation="MHIVDYFIICIYLIGILIVGLWFQRKASEGINSYFLGNRSLPWW
                     ALGASGMASNLDISGTMIIVALVYALGARGLYIEFRGGVTLIMAFLMIFMGKWNRRSK
                     VMTLAEWMEFRFGKGTEGRLARMITALTSILVTIAMVTYFAVGGGKFLDKFLGIPNFL
                     GLSSQFWAAAVLIAIAMIYTVASGLYGVVWTDVFQGSLIFLMILYVVIQVFHFDIPSE
                     FSVVMPLKDAAGGGFQFFPTTYQEWTDVLPKFKLSIDSSSVYSMYNLFGIAVLFYLLK
                     VVIEGCGGTGNYMIQRYYAAKDDREAGLLSLFWTFLLSFRWLFVGAMAVWGIYLGTQI
                     TDPEMVLPIVVQTLPIGIKGFLVAGMMAAAMSTFDSTVNAGAAYWVKDIYLEYINKNA
                     SDHQQMRQSYGASIIIVVLGLVLMLIIKNINEIWGWITMSIGAGLIVPQLLRWYWWRL
                     NGYGYTIGMVAGMLASIIWKIIAPEATAEYWSFLFAAGISTVATVIGTLLTEPTDEKV
                     LDNFYRVTRPFGFWGPIRAKLPEKLISQINRENRRDIISIFFAVPWQVCLFLMWMVLV
                     MKRYDTFLSLLLIVVTLSTILYFTWFRHLATEVNIESDADSSSG"
     gene            20824..21657
                     /locus_tag="PLI55_03070"
     CDS             20824..21657
                     /locus_tag="PLI55_03070"
                     /inference="COORDINATES: protein motif:HMM:NF022932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPA99850.1"
                     /translation="MDIFQDNVLHKMMQLGFTNYEARTYLGLLKLSPATGYEISQLAH
                     VPRSVVYSVLKQLESRGIVISIHDKPRRYIPLAPDEMLKRMETDYASRVSDLKDDLAE
                     LSNKSGTEGFWNLRGYQSLMQTCETLIREAHKSIYISGWQREVSLLREHLLNACKRNV
                     FVVIFSFNEINPPIGVTLAYGIPEEKLEHYWDRKLILVTDSKNLIMGPANQKEDEQAI
                     WTQNKAALTIATNYIILDITLFGQRMNMDISNIIAGMLTDEFEKVDDLIQEAIEKHKK
                     A"
     gene            21726..23501
                     /locus_tag="PLI55_03075"
     CDS             21726..23501
                     /locus_tag="PLI55_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPA99851.1"
                     /translation="MILNNCEINQNLQSLNGKWLFKYDRNGIGDTQGYYRTEYDRKDW
                     STINVPAFWDDPHYDGIGWYATEFKLKKPLGNNRLALVFEAVDDDAVVYLNGEKVGEH
                     TGYGIQFYLDITHQIKPETSNLLVVRINDTGGAGGITGNVLLRYYRDETELLQSEFSQ
                     EEANPVPDWVKSACLYEVFVRAYSPEGDFQALTRDLQRLSLLGVDCIWLMPIFPIGAK
                     HRKGSMGSPYSIRDFKSINPDLGTESDLKNLITSAHQLGIKVILDIACNHSSWDNLLI
                     EEHPNWYTQDAQGKIIAPNENWSDVADFNYNQTGLREYMWEVLEYWVKEFDIDGYRLD
                     VAELVPDDFWKIAAHRLQKIKPDILMLAEGDHPRLYLNGFHLTYGWNTRRAFYQIIKN
                     DYPAEFLAQTLRKEYYRYPQHALKMRFTENHDEARTMALFSPEQTMVLTFLIFTLPGV
                     PMIYAGQEIGVSEKPSLFEKDVINWRQFDKSLNELITKMNELRRNISALSSDTYQVIN
                     TSDPESIFAFSRYNAQEVVLIVTNLKSKSQMATIDLAPISAKIPNTEPELLVGTGVFS
                     LENNTLYLPCYGYDYYLFRFETKND"
     gene            23548..26427
                     /locus_tag="PLI55_03080"
     CDS             23548..26427
                     /locus_tag="PLI55_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPA99852.1"
                     /translation="MFFSAQVLIAQVVYSNPPHPTERDSIIIYFDATKGDGGLKDFTG
                     DVYAHTGVITNLSATAKDWRYVITPWPGQGGTANTSKNKLERISTNLYKLTIGFPRTF
                     YISHENGATIPDGEKILKLAFVFRNADGSKSGRDVGGADIFQNLFEVGLTVIVSEPVI
                     DLSLSDPRRSPVFTNYGDTVNIKITTAALNTKVSVIKLLVNHELINQVADTAMSFDFV
                     AEEYNYGMNNLMAIGTDTSGFSDTASFAIMINPPLTSISRPEGIVDGINVLNNNSITF
                     SLQAPFKDFVYLIGDFNDWLVDTSYFMHCEDNGGTDVHWWLTIDDLDSQTEYAFQYFV
                     DGEIRLADPYSEKILDPWNDDSISASVYPNLKPYPAGKTAEIVGTFQIDQPKYSWQTA
                     DYTRPEKTNLIIYEMLIRDFIDAHDFKTLIDTLDYLQRLGINAIELMPVSEFEGNSSW
                     GYNPSFYFAVDKYYGPADDFKHFVDECHRRGIAVIQDIVLNHSYGSSPLVRLYWDKVN
                     SRPSSENPWYNQVSPNPVYSWGYDFNHESQDTKNFIDRVTSYWLTDFRVDGFRLDFTK
                     GFTNKPGEGFAYDASRIAILKRIADKIWEIDTSAYVILEHFADNSEEKILADYGMMLW
                     GNSNWNYAQSSMGYTNESDFSWGFYKSRSWNQPHLVTYMESHDEERLMYKNLTWGNSS
                     GSYNIKTLNIALERIKMTAAFFLTLPGPKMLWQFGELGYDYSIEYNGRLGEKPIRWDY
                     YQDPDRLRLYKTIQALIKLRSENDVFTSASTTPTMTLSGNVKSIKMSGSNLNVVIIGN
                     FGVVNAETAVSFQHGGVWYDYFSGESFNFSGFDSVNLAPGEFHIYTDQKLATPEEGLV
                     AIAEADANNLPKQFQVYPNYPNPFNSLTTFEYDLEKISDVTIKIFDLLGREIYTKQIG
                     SQAPGKYRFVWNGIDKNSREVHSGIYFVIVQRGGNRQILKVTLLK"
BASE COUNT         7771 a         4856 c         5785 g         7834 t
ORIGIN      
        1 ggcgtggcgt ttgtggcaga gggtgatgga tttcagagcc gtttacgcat tctggtcggt
       61 atggatccgg aaatgactaa aattgtacaa atgaaaatat tatcccaagc cgaaactccc
      121 ggactcggga caaaaattga aaacgatcca acgaacaaaa cggatgcgtt ctggttttta
      181 aagcaatttg ctgatttacc agcagtaagc ccgatatccg ttgtcaaggg acaagccgca
      241 gataaaagta aatcgcaaat tcaagccatt accggcgcaa ccatttcttc gagggcggtt
      301 gtagatatta tcaacgccgc tattgaaaaa aaccgcgccc tttatttaga gaaggtgaaa
      361 taatatggcg aaatcaaaaa tttcagtaac acaggaattt ctcaaaggac tctggaacga
      421 gaatcccatc ctgcgtatgg ttttgggaac ctgcccaact ttagccgtta caacatcagc
      481 tattaatggc gttacgatgg gattggcagt aatatttacg ctggttatgt ccagcatgat
      541 gatctctctt ctaagaaaat tgattcccaa cgaagtccgg attgcgacgt ttatcatcat
      601 aattgcgact tttgtgacgg tggcagaccg agttctggcg gctttttatc ccgaaatgag
      661 caaggcgctg ggaccttttg ttcccttaat tgtcgtgaat tgcattgtat ttgcacgtca
      721 ggaagccttt gcctcaaaaa atccagttgg tcgttctgtt attgatgcgc tcggaatgag
      781 tgtcgggttt actctggtat tactgataat gggttcattt cgcgaactaa ttggtaatgg
      841 tactattttt tcgcatcaaa ttatgccatt gtcatttcaa ccctggatgg ctatagcttt
      901 gcaaccaggt gctttcttga cgttgggttt tatgattggt tttgctaatt ggcttaatac
      961 aaaaggtaag aaaaaatgaa tctgatatta attttcatct cagccgcggt tatcaataat
     1021 tatgtcttgg tgtcattcct cggaatttgt cccttcatcg gagtctcgaa aaaactcggt
     1081 tctgcggtta gtatgggaat ggcaacgacc ttcgtgatga cgataacggc cgcagttacc
     1141 tggttattga ataagtatat tctggtcggt ttacacctgg aattcctcca aattgtatct
     1201 ttcatcttgg taattgcctc gctggtccaa ttagttgaaa tgtttattaa aaaagtcagc
     1261 cgtccgctct atgatgcgct cggtattttt ctgccgctga tcacgaccaa ctgtgccatt
     1321 ctggggctgg cacttttcct ggtgatgaag caatataatt ttgttgagag tatcgtattt
     1381 ggttttgctt ccggggcagg ctttaccttg gcgctggtaa ttatggctgg catccgggaa
     1441 gagttggaac tggcggatgt gcctgaacct tttaaaggag caccgattac tttaatcgtc
     1501 gccggttgtt tagccctggc atttatggga ttttctggat taatctaatc tcaaaaggta
     1561 tctgaaatga attccacaac gtttttatac agtttattga gtatgggcgg gattggtttg
     1621 ttcttttcca tattaattgc agtagctaat aaaaaactac acgttgaaga agacccgaga
     1681 atcagcttga ttgctgagtg tttaccgggt gccaattgcg gcagttgcgg ctatgccggc
     1741 tgtaataatt ttgctgaggc tttagccggt ggtaaatcgc aaatctcaaa ttgtccggtc
     1801 tgcagtccat cagcccggga ggagatagcc cggattttgg gagtgagtgt cgaaattggc
     1861 gagcggcaag tggcggtggt tttttgtcag ggcggcgacg atgtcgctac taaaaaagcc
     1921 gagtatcgcg gcatcaaaac ttgttctgcg gctaatttgg ttagtggcgg tgaacgtgcc
     1981 tgcagttacg gatgtattgg acttggagat tgcgtgacgg tttgtccgtt tgacgctatt
     2041 catatgaatg aaaataactt accggtcgta gaccgcgcca aatgcaccgg ttgtggcaat
     2101 tgcgttactg cctgtccacg gggcattatc gaattgcatc caatcagtca tcatgttttt
     2161 gtgctttgta ggaatcgtga catcggtaga attgcccgta cggtttgtaa gcgtgcttgt
     2221 accggttgtc ttttgtgtga aaaagccgtc aacggccaag gcttcaaagt cgtggataat
     2281 cttgccgtgg ttgatcacga cgtttatacc aaggaactgg ttctgccgac cgaaaaatgc
     2341 ccaacgaaag ctattgtaat ccttggaact aaggatgaac aataacgacc aatatttcaa
     2401 aactgatgat gaaacggcga cggtgattga tgctgaccag cactgggtaa cggtcgaact
     2461 ggtagaaaaa gatacctgcc attcttgcgg tgcgaaaatg atctgccgcc ccaattctac
     2521 aggcaagcgc atactcaaaa tacctaatac cattaatgct aaagtgggcg atacggtttt
     2581 aatcgagcaa atcggtgcta atcagctaaa attaaccata gttcaatatg gtttaccttt
     2641 atttggtttt ctattcggag tactaatcgc taaccgattt attaaacata gcatttttgg
     2701 aattccagcg gacattgtgc agatgttttg tggaattata gtagttatct tgaccgggtt
     2761 tatcatttat ttttggagta aacgtaaagc caaaagtgga tttaatgtct ttcgtttgcg
     2821 tcagacatta aatggcaagt ttgaatgatt ccgcagaaga agtaatgctt aatttcaaaa
     2881 tccctattcc ttacttttta ttttgtgttt ttctgttgac aagttgccaa cctaaaagca
     2941 agctggtcaa ggctcaatca acgcgtttcg ctatgggaac gatcgtcgaa ataacggtat
     3001 tggatacgac cgaagccgaa gctaataacg ctctcgaagc cgggtttgcc gaaatcaacc
     3061 gcatttccgc ccaattctgg gaaggcaatc ccgagggnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnncaa ttgggtgcta aaaatcacca aaccaaaatt ggaatgggtg
     3241 cggttgccaa aggctacggt attgatcaag ctgtcgcagt cattgcaaag cataatgtcg
     3301 ctggtgcatt ggtcaatgcc ggcggagatt tacgagcact gaggcgtgta gatgggaaaa
     3361 agtggagaat cgggattcag gatccgcgtc aagccgataa tattctactt gtaattgaat
     3421 tcgacagtgg ggcagtggct acttccggag attatgagaa attttttatg tatgaaggca
     3481 aacgcattca tcatatctta aatccacgta ccggtttacc ggctgactcc tgtcaatcca
     3541 caactgtaat tgccgcaact gctgagcaag ctgacgcaat ggcaactgga ttatttgttt
     3601 tgggcgcttt agccggacaa aaagctctgg aaaacttccc caattgcgaa gtgctctgga
     3661 tttgcgccga tggccagaag gttcaatctg ccggttttga gaaatattta gcacagcact
     3721 agtctaagtt ctaaaaattt aaataacaat atttgagcaa ttcataattg atttctgaat
     3781 attcatttac attccttaat gcaatattaa taataaataa tcactgacat acaaaattag
     3841 cgatagaagt tttgccatta aaaaatttta ttggaatgat aagtcaatat tttatagatt
     3901 cagttattgg acagagtcag taaaaataaa ggatgcttag atggaagata ataaaccggt
     3961 tcagaaccct ttacgggaaa tcgttgatcc gtttgttaat ttgattaagg cgcctcgcgc
     4021 tttatgggga attaatttat cgtatttgct ggagggaatc acctattttg gtattctcag
     4081 tcttttagca atgtatttca atgatctcgg actgaacgat atacaggctg gacaaatggt
     4141 cgggattcaa actgccggga taacattggc aatgttattt ttaggagcga cggtagattg
     4201 gatcggagtg cggaaggctt tattgatttc actttcaacg atgctggtcg ggcgtgtact
     4261 tctagcattg gcacctcaaa tcggtgctcc cggactttgg caaaaagctc atatcgttgc
     4321 tatgttgggg atattggggg tagtcttagg ttatggcatt tatcagcctg cctgttatgc
     4381 cgcggtcaag caattcactg acaaaaaaac ttccgctatg ggctatgcga tgctctatgc
     4441 aattatgaat ctggggggct tcctgccagg attaatttcg ccgccaatca ggaaaaatca
     4501 tggaattgtc ggtgtatatt gggtttatgt agctttaacg gtagttggga ttttagtagt
     4561 tgccttcatt ttatcgaaga agaccgtcga gatggcgatt gccaatgctc gggaaggaca
     4621 ggacgaacca gtttctgatc cagaaacgac tgaaacgcaa tcaaccaagg aaaagattgt
     4681 ttattactta aaaaatttcc ccattaaaga cggccgattt atgtttttca ttttcattct
     4741 aattccggtt cagactctat ttgctcataa ttggctgact ctaccgatgt atttcgaacg
     4801 agctttttcg ggcgtggttt ccaacaattt tgaattcttt gtcaatttta atcctatcct
     4861 gatttttatt ctggctccaa tggtagcggc tttaaccgcc caaaaagaca cttataaaat
     4921 gatgatttta ggcactacta taatggctct accgactttt ttattggcaa ttggtccaaa
     4981 tatctactcg gtgtttggct atctaatcat tatgacaatc ggagaggcta tctggcaacc
     5041 gcgttttctg cagtgggtag ctgaaatcgc ccctaagaat atgaccggca tttatatggg
     5101 gattggacag tttccctggt ttctgaccaa agtcattacc tcgctttatt ctggctggtt
     5161 cctgacgaaa tattgcccag ccggagttac accggaacaa actaatactg agacgatgtg
     5221 gctgatttat ggctttatcg caatcattag tccgattgct ttattaatag caaggaaatg
     5281 gatgggtaaa ggactcgaaa aacaaaaagc ctaaatgcta ataaaaccca aaatattaat
     5341 cgttgatgat gaagcggtaa ttcgtgaaat ctacggggat ttcttggaac aaaatggatt
     5401 tgaagtggcg acagctttaa atgtcgaaac ggctttggaa tccataaagg ctaaaaagcc
     5461 ggatttaatt ctattagata ttgtgatgcc ttttcgtaac ggctttgatc tttacgacca
     5521 agtaatagtg gattaccctg gtattaaatt tgtctttatg accggttatg atgatgatat
     5581 aaagattact gaccgtttaa gcaaatctgg gaagaaatgg tttactaaac caattaaatt
     5641 agaagaaatg ttagaaataa ttcggaatga gttatcagct tcttaaactg atagattccg
     5701 gcggatatgt tttttaaaat attttatctt tatcggtaaa cagtgctcgt ttactaattt
     5761 ctgcacgatt ttgtagttat ccatagtccg aacgataact gtagcctcga aatgagacgt
     5821 ggtattttta tggcattcta ccagagaatt cannnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnagcgaacg tcgtagtgaa ttgagaactc gagcatagag aacattgaga
     6001 tcaaaaggct tggtaacgta atcatctgcg ccggtaacca atccatcaac ctgaaaatcg
     6061 gccgacgttt tcgcagtcaa aataatcacc gggataaaag ccgtgattgg acgattcttt
     6121 aaagtcttca ggactgaaag gccatcaatg tccggcaaat taatatcaag aataattaaa
     6181 tcgggttctt tactaatagc tagatcgagt acttgtgctc cttctgtagt cgcgtaaact
     6241 tcaaatccac gagatgaaaa ataggatgtg atcaattcta taatgttagg atcatcatca
     6301 actgctaaaa tttttctcat tactctataa tttcactaaa caattaatca ttatcaatat
     6361 aaaaagttat atgaaaaaag aaaatataat tttaagatga gtaaaactct gagaaaccat
     6421 tcactagttg tagattttcc ataaatttta ttgtaaccag gcttaaaata ttttgtctaa
     6481 cgaattatat ttgctatttg aatattggca taaaatttgt aaattaagtc ctgttctttg
     6541 atctaatgat aatgtccaaa agaagataga tttcccctcc ccctccccgg atgtgccgta
     6601 atatccctca aaatccccct cttttacgtt cggggaggga aacctcaaaa tcaccgaaac
     6661 actatagata agccgcttac aagagcggct tttttatttt atcaaaaatt cattatatat
     6721 gatcaaaaat tgataaattt ttataatcct tcgtagcatc cttatatcca cattttagaa
     6781 atagattaga ttaacgcgta atagtttttt gtaattcaca ggattgttga gcacatttaa
     6841 gcaagcttta ggctaatcat gtcaacactc cctcttcgag attaggtgga gttaccggta
     6901 atattgttca accccggcgg ggtttggcgt agtctttaga tttttgactc aagattgaat
     6961 cccgggtaaa gcaggtttaa ttcctaacgg agtttaaggt tattcaagat tagatttatt
     7021 gaagttatag atacatctaa tccaattttg acagtggggg attgtaatct tcccgcctat
     7081 tatcaccaaa agtgaatacc atatttttag ctcggacaaa aactagtaaa aaaaactatt
     7141 gacttaaata ttttaatacc ctatattctt ttagtagtaa ctcagattag aactacaatt
     7201 gaaaaacgcg acagaaaatc aagtcccaaa aggcaaattt tcactggcaa gaggattgct
     7261 tgtcgtcgga atagtagctg ggatttgttt gagtgacaac ctctcagata gaacatactg
     7321 gacttacgat gctatctggt atcaaatatt cccagagcgc tttaggaacg gtgatccgac
     7381 taatgatcca acgattgaaa cgttagaagg cacctggcca tatgaacctc aaacgcaatg
     7441 gagcattatg ccatggactg ctgattggta taaactccag ccttgggaaa aatccaacca
     7501 acgcgacttt tactataatg ctcaattgcg tcgttatggc ggtgatatcg agggaataat
     7561 tgataaactc gattatcttg tagatttagg aatcaatgca atttatttga atccagtctt
     7621 tgaatcggct tcgtcgcata aatacggtgc tacaatgtat catcatattg ataataattt
     7681 tgggcctgat ccggctggtg atatagcaat ttggcaaact gaaaatccta atgatccttt
     7741 gacctggcag tggacttccg ctgataaatt atttctgaaa ctaatacagg aggtgcatac
     7801 ccgtcaaatg aaaataatca ttgatggagt attcaatcat gttggcatac ctttctgggc
     7861 ttttcaggac gtcaaaaaaa atggagaagc cagtccatac gccgattggt ttgtgataaa
     7921 gtcttttgat gatccgagta caatcgaaga tgaatttgaa tatcaaggct ggaatggaat
     7981 tccggattta ccggagatct gggaggacga gaatggtcct gtacaaggat ttcgggcgca
     8041 tataaaagcc gtggttgaaa gatggggtga tccaaatggt gatggcgatc cgtcggacgg
     8101 gattgacggc tggcgattgg atgtggctga gaaggtcagt aaaaatttct ggcgtgattt
     8161 tcgcaaatgg gtgaaaagca taaatcccga ggcatatttg actggtgaaa tatggtggga
     8221 agattttatg aataataaga tgttcgatgc ttcaccatgg ttgcagggcg atatttttga
     8281 cggcgtgatg aattatcgtt tcggcgatgc tatgcttaaa gcctttattg acagagaaat
     8341 gaggataaaa ccatccgagc ttgatcaatt attaggcgat attagagaaa aatatccgct
     8401 acccgctcaa tatcaactgc aaaatataat tggtagtcac gataatgagc gttttgccag
     8461 tatgttatta aatccagacc gctggatcga tcacggtggc aatctggttt acaataaaga
     8521 ttttaagata aaccgtcctt cggatgagct ccgacaaatc caaaagacaa ttcttgtttt
     8581 tcaatttact tatatcggag caccttatat ttattatggc gatgaagtgg ggatgtgggg
     8641 agcagatgat cccgacaatc gaaaaccaat ggtttgggat gatatgcaat atgaaccgga
     8701 gactactcat ccatttggaa taaagagacc tgtagattcc gttaaagttg atacagattt
     8761 gttcaatttc tataaatctg tgataaatct cagaaaaaat catgaatgtc taagaagagg
     8821 caggtataaa acaatattga ctgacgataa aaaagggatt ttcggatttg agcgtaaata
     8881 tggtactgag accatctaca tctttgcaaa tttgaatgat aaacctatca aaatgagcga
     8941 gataaacttt gatgtcacaa aatgcgaaaa gatttttaca gttaataccg atacaaatta
     9001 tctcgggtcg aaaggaatag tagtttataa aattattgaa gggtaattgt ctttgataaa
     9061 atcaataaat caaattcaat tgaggtaaag gatgagatat aaggattggt taataacagt
     9121 tgttctgatt ttaactgcag ggtatttatt cggaggtaat actggtaaaa ttactggtat
     9181 agttactgat agtcaaactg gtcttccttt aataggttgt aacataatgc ttgaggatac
     9241 ttatttgggt gcggcatccg atacagatgg acgatatatg attcttaatg taccgcccgg
     9301 cgaatatcgt ttaaaagctc aaatgattgg ttaccagcca aaagtgataa ccggcgtgat
     9361 tgtatcagct gatttgacaa ctactatcaa tattgggctt tcgactcagg ttttagaggg
     9421 tgaagaagtg atagttactg cagagcgcaa aatgattgtc aaagatatga ctgcgaccgc
     9481 ttcaaacgtt aaagctgaag aattagccgc tttaccagtg actgaagtta gcgacgttct
     9541 tgaaatgcaa gccggatatg tagatgggag tttacgggga ggcagaagag gtgaagttgc
     9601 ctatttaata gatggtattc cggtaaccga ttcttatgat cgtagtgcgg tcattgatgt
     9661 caataaaaat atgattcagg aattgcaggt aatttccgga gcgtttaatg cagaatacgg
     9721 aaaggtaatg tcaggcatcg tcaatatcac taccaagcaa ggtagcaaca agcacggtgc
     9781 gtcgctagaa atgtatattg gagattatct tagtacacat gacgaaatct ttgataatat
     9841 tgggtcaata aatcctctaa atatccataa tgctgaaatc agtttttttg gaccattaat
     9901 aaaggataag ttattttaca atttcaatac gcgctatatt tactttggtg gctggctaca
     9961 tggtcagaat cgttataaac cgacgagtat attagctgtg tctcccgatg gttcgcattt
    10021 cttgctggac tcaactacta tgttaggaga tcaaaagtgg gttaatatga actggaacga
    10081 aaagttatat tttcaaggac aattaatcta ccggctttct aaccgaagta gtatttatta
    10141 caatttcttt tacgatagca aagattacca ggattatgat cgtatgttca aatataatcc
    10201 ggcaggaatg ttgaagaaaa acctaaccgc tgaaacgcat ttactaaagt ttcagcaaca
    10261 attaagttct aaaacctttt tcactctggc tggctcatat tattatcgta aatataccgg
    10321 cagattgtct gccgatacca gttacaacga tagcaccacc tatgtccacc cggcttacct
    10381 ttatcaatat ccttatcaat tttattttgg tggaactcaa aatgatcacg aatatcgtga
    10441 cagtagaaca taccttgcca aatgggattt gacttcccag ataaatggac aacatcagat
    10501 aaagggcggt ttggaatttc aacagcatga tatttcatta agaacattta atattcgtcc
    10561 agcgactggt gagacctata tagacttcgc atccggattg aatataccat ctacctggaa
    10621 tccatatatt catccgacta ttttggataa tagtacgatt tatgctagct attaccgtca
    10681 taaaccttat gaatttgccg gatatttaca agataaaatg gagtttagcg agttgatcat
    10741 taatatcggg gtaagatttg attattttga accggatggt gttgtgcttg cggatcccag
    10801 cgatcctgaa atctacgagc caatcaaacc tgaaaatcgt tatcatgatc ttaatggaaa
    10861 cggcgtccag gatcccggtg aacctgaagt cactttggag gaacgaagac aatattggta
    10921 taaaaaagcc actcccaagt atcaatttag tcccagaatc ggattgtcat ttccgattag
    10981 tgatcgcggg gtatttcatt tttcttatgg tcattttttc caaatcccca attttaacta
    11041 tttatatatg aatcctgatt ttgatcttgg ttctggaacc ggtaatcaag gagtagttgg
    11101 aaatgcggat ttgaagccag aacaaactat cgtgggagaa atcggagttc agcaacaatt
    11161 aagtgataat ttttcagttg acattactgc ctatctgaaa gacataagag acttgaccgg
    11221 aacgagagca gaagagatta tcgtttatgg cggagcggcc tcttatagcc gattagttaa
    11281 tagtgatttt ggtgtagtaa aaggtattac tatagcactt aatcagcaga atccgagcgg
    11341 tttatatacc agttttgatt atacgctcca aattgctaaa ggcactgcat cagatccgga
    11401 agcataccgc aatgcgattg ctggtggaaa ccaaccagaa attcaactta atcccttaga
    11461 ctgggatcaa cgccatacaa ttaatggaac agtgggatat aatcacaaaa attatggaat
    11521 aaattttatc ggtcgctggg gaagtggttt gccctatact ccgcaatcaa gcgaagatat
    11581 tacatcactt ttaactaatg ctggtactaa accacctacc ctaacaattg atgctagatc
    11641 ttactatcgt ttcaccttat caggaagata ttcggttgaa ttatttatca gaattcagaa
    11701 tttatttgat aaattgaatg aagttaatgt ttatgacgag accggtcgag ctggttttac
    11761 gaccgatgaa gcccgagtag aagctttaaa tattaaaact ccgattaatt cggttcatga
    11821 gtggtttaac gacccaacgt attattccga accacgtcga gtcgaaatag gaatgaggat
    11881 aagtatatga aacattcgat aaaatatctt actattgaga ttctctcaat tttgttaatg
    11941 acgattgtaa cgtcacaaat aatttttgcc aatcaaagtg ggaaagctta ccggcgtagt
    12001 tccatccata acggcaatct ggttcggaca gtttttggca actggggagt catcggccaa
    12061 ccaagggaaa aaggtccgcg tggggcatgg ctttttgata ccaatggata tattggcgat
    12121 gtcagtccaa tggtaggcgc tgaagtaaat tatcattctg aagaacttga ctcgacaata
    12181 atattccata gcgttgtagt ttgtccggta gatcgtccgg caactgctcg cgaagaatcg
    12241 cctactggtg atcaatggac ttttgagccg gtttccggtt attttaatga gtcttctcaa
    12301 ggaatagcta tgagcaccga ccgatattcc tggccgcctt attggcctga taaaatggat
    12361 gatcctgagg atccgggttg gccagaacaa tggaacggat tttttggaaa aggggtaacc
    12421 aatgctgatc aagaaagtta ttttattatg gatgataata atgatgttga attcaacagt
    12481 ggtcacaatc taaagggtgt ctatttcaaa cctgataata gagatgcgaa tcgcaacgga
    12541 ctcggcttgg aagtgaaggt tcggggaatg caatgggctc agtttctggc acaggatgta
    12601 atattttggc tttatgaaat taccaataca agcactacgg attatgataa agtagtcttc
    12661 ggaatgatag ccggaactta caacggagtg actggtacag atgattcacc tcaggaatat
    12721 gatgacgatt attctttctt tgatgttgaa cgggatttga cttatactgg tgattaccct
    12781 aataataata gtcgtaatcc taaatggcag ggaccggttg gactggtcgg atatgctttt
    12841 ctggaaagtc ccggtaatcc ttatgacggt attgataatg acggtgataa taaacctgaa
    12901 gaacatcctg gtaaaaattt aatttttacg gcacccctat ttaccgaatc agatttcgac
    12961 tcagttgtat ataataaagg agaccagata gtattaatcg acaaagatta taagcgtact
    13021 tttcacacaa ttaccagtga tacagaaacg gttgtaacca aagggatgca aatcacaatc
    13081 attgcgggac aaacaaaatt agtagaagga aatgtcctgg caaatggttc aattaatcca
    13141 aatgtttatg acggcattga taatgatctg gacggattaa ttgacgagaa ttacctgttg
    13201 cattatagac aacgccgggt ggatcaaaac ggagtagttt tatttgacac cttaaatccg
    13261 gtagcctata aagattatcg aaggggattg ggaattgatg atcctttgat tgacgaagcc
    13321 cgcaatgatg gacttgataa tgacggtgat tggaatcctg aaacagatga tgtcggtagt
    13381 gatggtaaac caggtacaaa tgattatgga gagaatgacg gcaagcctac actcggtgaa
    13441 ccgaatttcg ataaaaccga tgttgatgaa tcggaccaaa tcggtttaac tagttttaat
    13501 tatttcgttc catctaatgc ttatccaatg gcggatgatg aattactctg ggaatggttg
    13561 aaaccgggat attttgatgt gccgtcgagt attcaaaatg gcaaaccagt tgccggggaa
    13621 gacggcgatt tcatttatgg ttcggggtat tttccactac gagccggtga tacccagcgt
    13681 ttttcattgg ctttagtata tggctatgat ttgaccgatc tactcaagaa tcgggaaacg
    13741 gttcaggata tttacaacag tgattatcgt tttccccaac cgccggataa gccgacggta
    13801 accgccgtag ccggtgacgg caaggtaacc ctttattggg atagaaaggc tgaagaaagc
    13861 atcgatccag taactaagga gaaagatttc gaaggttata aaatctataa agctactgat
    13921 cctgatttca atgatgtatt tgttattacc gatgccaatg gtgtgctgac tggttacaag
    13981 ccactggctc aatttgataa ggtcaatgga atcaaaggaa ttttcgaggc gagttcggat
    14041 ttgtatcaaa gtgctggcgg ttattcttac tatttaggct ctgacaatgg tttagtgcat
    14101 tcctacgttg atgaagatgt tcagaacgga cgaatctact attatgcggt agtcgcctat
    14161 gatcacggtg acgccagtaa tgatattttt ccatcggaaa ataccaagtt tatttcggtg
    14221 cagtcagatg ggacggtaat taccgatata aacaccgttc aagttagacc aactgcgaaa
    14281 gttgccggtt atgatgaaca aaatagtgaa ttagccctga aacacctgga aggtccggca
    14341 accggcgtta ttcagtataa aattattgat gaaacactat taaatgggca tacctataaa
    14401 gtgaatttca ccgacactcg aaccgacgga attgataatg atgacgattg gaattattta
    14461 acttgcgatg ttggctctga tggcaaacca ggtacaaatg attatggaga aggggatggc
    14521 atacctacga gcggtgagcc gaattttgaa tggaatgatg aagatgaatt tgctcaaatt
    14581 acatccactt actctgtttt agataaaaca ggcgtcgaag aatttatcaa tcttgacact
    14641 tcttatgttt ggcttaccca tcaaaatatt caatatgacg atattttaat tgccagtgag
    14701 tcagatccga ataactattt atcattaagt aaattcgaaa tagatacctt acgaggaaaa
    14761 attaaaataa aagcggatag taacctaaaa agtggtagtt atcgtatatt ttataagtat
    14821 tatcctgtct ttaaaagtcc aaacattcag tatagtcctt atatgaaaga aacaaaagac
    14881 tccgatattt tcgatggcgt tgagttagtg tttagtaatc cgtgggatat taaaatgatt
    14941 aaatctaagt cgggctggaa ttcgactgat agaattattg attttacctt tagaattgat
    15001 caggctttat tggaaggcga taccatagtt ggagtacaat atcccggaat atatgagctg
    15061 cgagtcggtg atagtagcga agtattatat gaaactccca aagaattctt aaagtccatc
    15121 tatcctagtt tctcagatgc aattataaga accctggtaa agaagactaa tttttatcta
    15181 tttaatgtgt tggattctgt aaagatacct tttctttata atggtggaat aaaaaacagt
    15241 tatggaattt atgaaataac gccaggtggc ttgattgaat cttatattcc taaaccggat
    15301 agcacttatc aatattcgtg ggtgataaac tttccgcctg tttcagagaa agttacaccg
    15361 ccccggttcc taaaaggtga cattctccat attgaaacgg aaatcccctt ccgtaatggt
    15421 gatatttatg aattcactac tgaaattcct aatgtagatg atgaagtggc taaatctagc
    15481 ttagatgata ttcaggttgt ccctaatcct tatgtggttg ccaatacgat ggaatcgcct
    15541 ttaccgcctg ccattaccag tggccgtggg gaaaggcgga ttgaatttcg caaattgcct
    15601 aacgatgcca aagtacatat ctttacatca gccggggcgc atgtaattac cctaaatcac
    15661 aacggcaata ttcataatgg tactttagcc tggaatgtta aatcgaaaga gaatctcgat
    15721 gtagcatttg gggtttatta ctatatcata gaatcatcgg tcggtaaaaa atccggcaaa
    15781 ttagcagtaa taaaatagcg gtgagaacat gaatagaaaa ttacctacta taatttcgac
    15841 agtactgatt agcactatat ttttaaatgc tcagaataaa gaaggaactg ccgccggtca
    15901 atttttgcaa attccagtcg gtgggaaggc aattgcaatg ggtggcgcat tcacggctct
    15961 ggccgatgat gcgagtgcaa tttattataa tccgggagca atttcccgaa ttagtaaaaa
    16021 taaattgtat gtttctcaaa ctaattggtt agtgggaggg aagcatcaat ttatagcggc
    16081 tcaggtaatg gcaaccagca atgatatcat tggaattggc gttgattact tagattacgg
    16141 tgagcgagaa aaaattacta cggtagcgga accggatgga accggcgaat attgggaagc
    16201 aagtgattta gcttttagct tgggctatgc tcgcaactta accgaccgct tttcaatcgg
    16261 aggcagtgtc aaattcattc aacaaaatat ctggcatgtc agcgcaacgg gatttgccgc
    16321 tgatctggga ttattattta aaactcaatt caatggactt cagattggtg ccagcatgcg
    16381 gaattttggt agcgagttac aattaacagg acgcgattta attactcagg ttgatcttga
    16441 cgaagaagcc tctggcaata acgagacaat cgtcgcttat cttaaaactg ataaatggcc
    16501 aattccttta acgttcacta taggagttgc gatgccggtg atcaatagta atcaatttgt
    16561 tttgacactg gcaggtgatg tcgtacgtcc tacggataac agccagacac ttaactgtgg
    16621 agctgaagca ataataatga aaatgattag tcttcgtgcc ggttatcaag ctttatttcg
    16681 cgaaaagaca cagcaaggat tgaccgctgg tattggcttg aaattgccaa tccaatatgc
    16741 cgatttttat tttgattatt cttatcaaga atttggattt tttggggatc tttcaacaat
    16801 gtctatatca attggttttt aacaataaaa gaaaggagta agctatgaaa aaagctactg
    16861 ttttgatgtt gatgttgact ctaattgctg tctcgggatt agccgatgtg acagttaatt
    16921 atattgtcaa tacatcaact ctgcaagggg tcaccgattc gacccatcag gtccagattt
    16981 gtggtgcgga agtcgggcca gaaggtgttg ataagtggta caacctcttt ttgacctggg
    17041 gcgatgattc accactagcc gcaaatattg gaggagatta ctggaagctg acgattgcct
    17101 atccggattc gatgatcggc tggcggatgg cttataaggt tcgttataag gctaatgatg
    17161 atgatgcctt tacctgggaa aatgtcactg gtggaggtaa tcgtatgtgg gttttaccag
    17221 ctaatgacac aaccctggcg gtggcgtatg ttaacaatga tttcaatccg ccatttacac
    17281 cgtctgattc cgttgatatt ttagttcgag taaatatgga gaattacgaa gacagtttct
    17341 cactattgtc ggttgttggc cattggcctt tctgtgatcc agaatggacg cctggggcac
    17401 tgcctttgac tcaagaaggc acctccgatt actggagcag aacgatccgg attccacagg
    17461 ctgtgatgga taccgttgat cgagtacaac ctctcggtgc agagcatccc ggggtactaa
    17521 tgtatcgttt tgcgatcggt aatgattgga acaattcaga aaacttgaat ggtaaatact
    17581 ggccaggtaa tgaaaaccgg atcattgctc tacatcctgg tctggaagac acaaccctag
    17641 cttggaagta ctggaatgat gcggcgccag ccggttttga agcggaggat accgttgata
    17701 ttacttttac cgccgatttg actaatgcca ttgccaataa aggttttgca gttggcgata
    17761 ccattttagt ccgaatgggt tattttaaca gtctgtcttc aacaatggat gctaatctgc
    17821 ttcgaatttc cggaacctac ctatattcga ccacttttac agatgttcca gtagttctta
    17881 acgaaccgct ttattaccag tattatcttg taaaagatgc cgtagaacaa cgtgaagtat
    17941 atttcaattt tgattatgaa ggcgaagacg ttacgcgagc tgaaagacgt gcatacgtca
    18001 taaccggcga tgctgaaatc aacgacatcg aggatagcga aaccgatcca cgtcgcatgc
    18061 caaaattcag gaataaccag aaacttagtc aagctgttac ggtaacaatt gaatgcgacc
    18121 tgcgtccggc ctattatcaa gtctgggctg gttcgacgct tgacgatatt caaggcaaca
    18181 ttgacattac cccagcgatg ctggaagcca atccagatac tctcgctaaa ttaggtttat
    18241 gtatcaatgg cccaatgtcc aacaatggcg aaggtacctg ggcaacttgg ggtggaacgt
    18301 tagccagtga cgctactcgc agaatgtatg acgatggaac taatggtgac gagactgctg
    18361 gtgataccat ttatacgatt gtatacaatt ttaccactga aaaaaccatc ggtcaggaat
    18421 tcaaattcgg gattggcggc ggagataatg aaagcagtta tggtcttaac catatagaaa
    18481 atattgatga cacgaatccg acttttacac tggaggttca gtttggctcg atcaatccga
    18541 atttctattc ggcttgggat tttaatgcca gagcaccaaa agttggtatt aagaatcaaa
    18601 atgttagtgt tgctaaggag ttttctttgt ctcagaattt cccaaatcca ttcaatccaa
    18661 ctacagaaat tcgttttact atctcggaaa aaagcgatgt tcatttgacg atatacaacc
    18721 tgctcggtaa ggttgtcaat aaggttactt ataataacct ggacatcggc aattacagct
    18781 acgtctggaa tagtaaagac ctaaacggca acagtgttgc ctccggtatt tatttctatg
    18841 aactacgggc tggtagtaaa tatcatgcta tcaaaaagat ggttttgatg aaataagttg
    18901 ctcttaagag gaaggacgga ttttccgtcc ttcctctttt tctttgattg ttcattaagg
    18961 agatcgccag atgcatatag tagattattt tattatctgt atttatctga taggaatcct
    19021 catagtgggt ttatggttcc agcgtaaagc ctctgagggt attaattcat attttctggg
    19081 taatcggtca ttgccttggt gggctttagg agcatccggc atggcatcca atcttgatat
    19141 aagcggaact atgattattg ttgccctggt ctatgcctta ggagcccgag gtttatatat
    19201 cgaatttcgg gggggtgtta ccttgattat ggcattttta atgatattta tgggtaaatg
    19261 gaaccggcgt tcaaaagtaa tgactttagc ggaatggatg gaattccgtt ttgggaaagg
    19321 tacagagggg cgcttagcgc ggatgattac ggcattgacc tcgattctgg tcacaattgc
    19381 gatggtaact tattttgctg tgggtggagg aaaattttta gacaaatttt taggtattcc
    19441 aaattttttg ggtttatcgt cacaattttg ggcggcggcg gttctgatcg ccattgcgat
    19501 gatttatacg gttgcttccg gattatatgg agtggtgtgg acagacgtat ttcaaggttc
    19561 actgatattt ttaatgattt tatacgtagt tatccaggtg tttcattttg atataccgtc
    19621 tgaattttct gtagttatgc ctttgaagga tgctgcagga ggtggattcc agtttttccc
    19681 cacaacttat caggaatgga ccgacgttct ccccaaattc aaattatcga tcgattccag
    19741 cagtgtctat agtatgtaca atttattcgg aatcgctgta ctgttttatt tgcttaaggt
    19801 agttatcgag ggttgtggag ggaccggcaa ctatatgatt caacgttact acgccgctaa
    19861 agatgatcgt gaagctggat tactctcgtt attttggaca tttttattat cattccgctg
    19921 gctttttgtg ggagctatgg cagtctgggg aatatatctc ggaactcaaa ttaccgatcc
    19981 tgagatggtt ttacccattg tggtccaaac tttaccaatt ggaataaaag gatttctggt
    20041 ggctggtatg atggcggcgg caatgtcaac tttcgactcg actgttaatg ctggtgcggc
    20101 ttattgggtc aaagatattt atcttgaata tataaataaa aatgcttctg atcatcagca
    20161 aatgcggcaa agctatggag cctcgataat tattgtagtt cttggactgg ttctaatgct
    20221 tattattaag aatattaatg aaatttgggg ctggattact atgagtatag gagccggctt
    20281 gattgtgccc caattactgc gctggtattg gtggcgacta aatggttatg gttatactat
    20341 cggtatggtt gctggaatgc tagcttcgat catctggaaa attatcgccc ctgaagcaac
    20401 cgccgaatac tggtcatttc tatttgccgc cggaattagt accgtcgcta cggttatcgg
    20461 caccctttta accgaaccaa ctgacgaaaa agtattagat aatttctacc gagttacccg
    20521 tccatttggt ttttggggac caatccgagc taaattaccc gaaaaactga tatctcaaat
    20581 caatcgtgaa aatcgacgcg atataatctc aattttcttt gccgtgcctt ggcaggtgtg
    20641 cctgtttttg atgtggatgg ttctagtgat gaaacgctat gatactttcc ttagcttatt
    20701 actgattgta gtaaccctat caacaatatt atactttacc tggtttcgtc atcttgcgac
    20761 agaagtaaac atcgaatccg atgccgattc atcttcgggg taacctaaaa tagaaagaaa
    20821 aaaatggata tttttcagga taatgttctt cataaaatga tgcaactggg ctttactaac
    20881 tatgaagccc gcacttacct cggactactg aaattgagtc ctgctaccgg ttatgaaatc
    20941 agtcaattgg ctcacgttcc tcgatccgta gtttactcgg tattaaagca gttagaatcg
    21001 cgtggtattg taatttcgat tcatgataaa ccgcgccgat atatcccttt ggcaccggac
    21061 gagatgttga agcggatgga gaccgattat gccagtcgag tatcggacct taaagacgac
    21121 ttagccgaac tcagtaataa atcaggcacc gagggattct ggaacttacg tgggtaccaa
    21181 tctttaatgc aaacctgtga gacgttaatc cgcgaagctc ataaatctat ctacatttcc
    21241 ggctggcaac gtgaggtttc gcttcttaga gagcacttac taaatgcctg caaacgcaat
    21301 gtttttgtcg taattttttc ctttaatgaa attaatccgc caatcggagt aaccttggct
    21361 tatggcattc cagaagaaaa actggaacat tattgggatc gaaaattaat tttggtaacc
    21421 gattcgaaaa atttaattat gggaccagct aatcagaaag aagatgagca agccatctgg
    21481 actcagaata aagcggctct gacaattgct accaattata ttattctgga tataaccctt
    21541 ttcggtcagc gaatgaatat ggatatttct aacattattg cgggtatgtt gactgacgaa
    21601 tttgaaaaag tcgatgatct cattcaagaa gccattgaaa aacacaaaaa ggcttaagac
    21661 tccaaccgat ttaacctgga atgaccaata tgcctcgagt gttaccttta atttttgcat
    21721 tacttatgat tttaaataat tgtgaaataa accagaattt gcagagtttg aacggcaaat
    21781 ggttgttcaa gtacgatcgt aacggcatcg gtgatactca aggttattac cgcaccgaat
    21841 atgatcggaa agactggagc actataaatg ttccagcctt ctgggatgat ccgcattatg
    21901 acggaatcgg ttggtacgcg actgaattta aacttaaaaa accgctgggt aataaccgac
    21961 tggcacttgt ttttgaggcg gttgacgatg acgccgttgt atatctaaac ggcgaaaaag
    22021 ttggcgaaca taccggatac ggaatccaat tttaccttga tataactcat caaatcaaac
    22081 cggaaacatc caatttatta gtcgtgcgaa tcaatgatac cggtggagca ggcggtatta
    22141 ccggtaatgt tcttttgcgg tactataggg atgagacaga acttctacaa tcagaatttt
    22201 ctcaagaaga agcaaatcca gttcctgatt gggtaaaatc agcttgcctt tatgaagtat
    22261 tcgtcagagc ctatagtccg gaaggcgact ttcaggcact aaccagagat ttacaacgtc
    22321 tgagtttgct cggagtagat tgtatctggt tgatgccgat ttttccaatc ggcgctaaac
    22381 accggaaagg ctcaatgggc agtccctatt caatccgtga cttcaagagt atcaatccgg
    22441 atttaggaac agaatcggat ttgaaaaatc tcatcaccag cgctcatcaa ctgggaataa
    22501 aggttattct cgatattgct tgcaatcaca gctcctggga taatctactt atagaagaac
    22561 accccaattg gtacactcaa gatgcgcaag gtaaaataat tgccccgaat gagaattgga
    22621 gtgacgtagc cgattttaat tataatcaaa ccggtttgcg tgaatatatg tgggaggtat
    22681 tagaatattg ggtcaaagag tttgatatag atggataccg gctcgatgtc gctgaactgg
    22741 taccggatga tttctggaaa atagccgcgc accgcctcca aaaaatcaaa ccggatattt
    22801 taatgctggc ggaaggcgat catcctcgtt tgtatcttaa tggttttcat ttgacctatg
    22861 gttggaatac aaggcgggct ttttatcaga ttattaagaa tgattatcca gccgaatttt
    22921 tagcccaaac tctaagaaaa gaatattatc ggtatcctca acatgcgctc aaaatgcgtt
    22981 ttaccgaaaa tcatgatgaa gcgcgaacta tggctttatt ttcacctgaa cagactatgg
    23041 tcttaacctt cctgatattt accttgccgg gtgtgccgat gatttatgct ggacaggaaa
    23101 ttggcgttag cgaaaagcca tctctattcg agaaagatgt tattaactgg cgtcagtttg
    23161 acaaaagttt aaatgaatta atcacgaaaa tgaatgaact gcggcgcaac atttctgctc
    23221 tatcatcgga cacctaccag gttataaata cttccgaccc ggagagcatc ttcgcatttt
    23281 ctcgatataa tgctcaagaa gttgtactta ttgttaccaa tttgaaaagt aaatctcaga
    23341 tggcgacgat tgatttggcg ccgatttcag cgaaaatccc caatactgaa ccagaattat
    23401 tggtcggaac tggagtattt tcgctggaaa ataatacatt atatttgccg tgttacggat
    23461 atgattatta tttattccgg tttgagacca aaaatgatta ataaccacct taacagaatt
    23521 ttttacaaaa taggcgtact atgcttgatg ttctttagtg ctcaagttct gattgctcag
    23581 gtggtttact ctaacccgcc tcatccgacc gaacgggatt cgatcataat ttacttcgat
    23641 gccactaaag gcgacggcgg tttgaaagat ttcacgggcg atgtttatgc gcataccggc
    23701 gttattacca atttaagcgc aaccgctaaa gactggcgct atgtcataac tccttggccc
    23761 gggcaaggtg gtacagctaa tacttcgaaa aataaactcg agagaatttc aaccaacttg
    23821 tacaaactga cgatcggctt cccgcgtact ttttacattt ctcatgaaaa tggcgcaact
    23881 attcccgatg gcgaaaaaat tctcaaatta gccttcgtct tccgcaatgc tgatggcagt
    23941 aagagtggga gggatgttgg tggagccgac attttccaaa acttatttga agtaggattg
    24001 acggtaattg tcagtgaacc cgtcattgat ttaagcctat ccgacccgcg tcgctcacct
    24061 gtttttacga attatggcga tacggtaaac ataaaaatta ccacagccgc gctcaatact
    24121 aaggttagtg tcataaaact attagttaat catgagttaa tcaatcaggt cgctgacact
    24181 gcgatgagtt ttgactttgt ggcagaggaa tataattatg gaatgaataa tctaatggcg
    24241 attggtactg acacgagtgg attcagcgat accgccagtt ttgcgattat gataaatcca
    24301 ccgctgacgt ctatttctcg acccgaaggt attgttgacg gtatcaatgt gctaaataac
    24361 aacagcatta ccttttcact ccaagcgcct tttaaggatt tcgtctatct gattggcgac
    24421 tttaatgatt ggctggttga cacgagctat tttatgcatt gcgaagacaa cggcggaacc
    24481 gacgtgcact ggtggctgac cattgacgat ctcgattcac agaccgaata tgctttccaa
    24541 tattttgttg atggtgaaat ccgcctggcg gatccctatt ccgaaaaaat tctggatcca
    24601 tggaatgatg actctatttc agcatccgtc tatcccaatc tgaaacctta tccagccgga
    24661 aaaacggcgg aaatcgtcgg cacttttcaa attgaccaac ccaaatatag ttggcaaacc
    24721 gcagattaca ctcgtccgga aaaaaccaac ctgatcattt atgagatgct aatccgggat
    24781 tttattgacg cgcatgattt taaaactttg attgatacgt tggactattt acagcgactg
    24841 ggtattaacg ccatagaatt aatgccggtt agtgaatttg aaggcaattc atcctggggc
    24901 tacaacccgt cattttattt cgctgtggat aagtattacg gaccggcaga tgactttaaa
    24961 cactttgtag atgaatgtca tcggcgcggc atagccgtta ttcaggacat tgtgctgaat
    25021 cattcctacg gctcgtcgcc gctggtacgg ctttattggg acaaggtaaa tagccgacca
    25081 tcatcagaaa atccctggta taatcaggtc tcgccgaatc ccgtttactc ctggggttat
    25141 gattttaacc atgagagtca agatactaag aattttattg accgagttac atcctactgg
    25201 ctgacggatt tcagagttga cggctttcgt ctggatttta cgaaaggttt caccaataaa
    25261 cccggcgaag gttttgctta tgacgccagc cggattgcca ttttgaaacg tatcgccgat
    25321 aaaatctggg aaattgatac cagcgcttat gttattctgg aacatttcgc tgataatagt
    25381 gaggagaaaa ttctggctga ttatggaatg atgctctggg gcaattcaaa ttggaattat
    25441 gcccaatcat caatgggtta tactaatgaa tctgatttct cgtggggttt ttataaaagt
    25501 cgttcttgga accaaccgca tctagttact tatatggaaa gtcatgacga agaacgcctg
    25561 atgtacaaaa acctaacctg gggcaattct tccggctctt acaatattaa aactttgaac
    25621 atagcgctgg agcggattaa aatgactgcc gcctttttcc tgactttgcc cggtccgaaa
    25681 atgctctggc aattcggcga actgggatat gattattcca tcgaatataa tggacgtctg
    25741 ggggagaaac cgattcgctg ggattattat caggatccgg atcgcttacg attgtataaa
    25801 actattcagg ctttgataaa actaaggtcg gaaaatgacg tctttaccag cgccagcacc
    25861 acgccaacta tgacactaag cggcaatgtc aagagcatta aaatgagcgg cagtaatctg
    25921 aatgtagtta tcatcggcaa ctttggagtt gttaatgctg aaactgcagt ttcttttcaa
    25981 catggcggcg tctggtacga ttatttctcc ggggaaagct tcaattttag cggatttgat
    26041 tcagtaaacc tggcgccggg agaatttcac atttataccg atcaaaagct ggcgacaccc
    26101 gaagaaggat tagttgctat agctgaagcc gacgcaaaca atttacctaa acagttccaa
    26161 gtctatccga actatcccaa tccattcaat tcattaacga cttttgaata tgatttggaa
    26221 aagatttcgg atgttaccat aaaaattttc gacctcctcg gcagagaaat ttatacgaaa
    26281 caaataggaa gccaagctcc tggaaaatat cgctttgtct ggaacggcat tgataaaaac
    26341 agtcgcgaag ttcattcggg aatttatttt gttatagttc agcgcggcgg caatcggcaa
    26401 attcttaaag tgactctgct gaagtaacca gcgttgacag ccggta
//
LOCUS       DAOOMU010000019        26205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1709_length_26205_cov_20.774914, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000019 DAOOMU010000000
VERSION     DAOOMU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 26205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26205
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1709_length_26205_cov_20.774914"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLI55_03085"
     CDS             complement(<1..626)
                     /locus_tag="PLI55_03085"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HPA99853.1"
                     /translation="MKELVSLAESAARAAGAILRSYFQQEYTITHKGLDDPVTNADTE
                     ADAFLKEKLLSACPDYGWLSEESADSAERLTKSRVWIVDPLDGTKEFIAGRPEFMVSI
                     GLAENGQPVLGVLYNPINDELFSAYKRQGAFLNGSRIFSTKTKTLSEARLIVSRSELT
                     ENLWQPYRHFFKTLQPCGSVAYKLALVASGKADIHISLKPKNEWDICAA"
     gene            complement(648..1925)
                     /locus_tag="PLI55_03090"
     CDS             complement(648..1925)
                     /locus_tag="PLI55_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="L-dopachrome tautomerase-related protein"
                     /protein_id="HPA99854.1"
                     /translation="MFKKGLILLILASAVFAQSNPPAIRPDSVGIYFNMSIQAYSEQQ
                     YDRYLEYTQKLIQVYPDDFTLQYNLASAYALNKDTVNAIKILNSLVDKGLGLVAEDDP
                     DFADLKNTPEFRALVKKIEKVKKPVKSSKKAFTVAEKDLIPRGITYDSQTKNLYISSL
                     YKSKIVQIDRNGKISDFVPERQDGLGPVTAIQADPQRRILWALSSFGIPNEKTPRELA
                     GTASVYKYNLNDGKLLKKYNLPRPNRHFLTDLTVDRAGNLYIADSGQRTIFRLDAQKD
                     TLEKFLELYKYPQLASLTITPDDKHLFVTYGGGILNIDVATGKITPLTSPDHILLVGC
                     DGLNFYNNSLIAIQTFLNRIVRFELSPDFSSVTDYKIIESNNPDFNIPSGGAIDGKYF
                     YYVANSQINSLDRSGNVMTPEQLKDILIFKAKL"
     gene            complement(1932..2549)
                     /locus_tag="PLI55_03095"
     CDS             complement(1932..2549)
                     /locus_tag="PLI55_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPA99855.1"
                     /translation="MNLLDNLTWLGHSSFRIAGKKIIYIDPFQVKAGIPADLILVTHD
                     HYDHFDRNSITNIKKNDTIFVAPASVCRQISGDTRPVRPGAQMNFDSVTIKAVPAYNI
                     GKKFHPRENQFVGYIIHADNIIYYHAGDTDLIPEMQDIRADVALLPIGGTYTMDAIEA
                     AQATKIIHPQVAVPMHWGTVSGSRADAEKFAQLANCQVVILNKGK"
     gene            complement(2568..3347)
                     /locus_tag="PLI55_03100"
     CDS             complement(2568..3347)
                     /locus_tag="PLI55_03100"
                     /inference="COORDINATES: protein motif:HMM:NF019003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HPA99856.1"
                     /translation="MRKTDFSPLPERKQLLYEQMARSYRTQERQKNTPWAPFEKRLIE
                     SKIAVISIAGAYLNGQKPFTNRETESDYQCREIPLNFNREDLRFFATDWEPSEALADY
                     NVVLPIDRLVLLQKEGMIGKIHETIISIAGFTKSIKMFGKFLTKTYDTLKKAEVDGVL
                     ILPCSAQTSAAACQVANYLEKKELPTVILTTFYEQALALAPPRCAFINFPFGRPFGKA
                     NHVTLHTAILRDTLRLFEKAKTPGEVICLSYIWSFGTVPNW"
     gene            complement(3398..4126)
                     /locus_tag="PLI55_03105"
     CDS             complement(3398..4126)
                     /locus_tag="PLI55_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPA99857.1"
                     /translation="MQNLIYVGRWLVGLVYFLLLGMTLRFGLIFWKPAEIYVLVQQIC
                     RAALRTIGIRIKVSGLDRFDHQQSYLIICNHESLLDAFICPAYIPMYFTTIELADHFK
                     WPLWGWMIRNWGNIPLQHGQLKSSLTSLDQAATLLKNGTSILIFPEGSRTTDGKLLSF
                     KKGAFYLAYRAKADILPLAINGLWRAKTRGDWRLRSANVSLNFGEPIRYSTYQHLSPD
                     ELRNLCFETIQQLKSPVASNVLSS"
     gene            4317..5765
                     /locus_tag="PLI55_03110"
     CDS             4317..5765
                     /locus_tag="PLI55_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPA99858.1"
                     /translation="MKIRNIIAVLGSLFTILGLFLILPLLISLFQHDAATPAFLFSIA
                     VATGVGLLMQIRKPRNFNIQRWEAMVIVVIGWIGASVIGALPFYFSGYFDGFINALFE
                     SVSGFTTTGASILNDIESLPISLLVWRTLTHWIGGMGIIILALVVLPAINISGRNLYS
                     FESSSLANEQITPKIKDTARLLWTVYFILTVSETVLLLVLGLPFFDAALTAMATIATG
                     GFSPKNASIAAYNNVYVEIVVVIFMILSGINFTLYLRMFQSRKNWRDYSETVYIYFAI
                     MAVASLVIVINLRSHTYNSWSEAIRYGVFQAVSINTTTGFVTTDWEIWQPVTHIVLLI
                     LMLIGACPGSTCGAIKNSRIILLIKSIHRELIQIIHPRVVTTIHFNKRPIDEKTIQNA
                     IVYIGMYLCFWLVASMIVALGGVAPESALIAVATTLGGVGPGIGLFGPIESFAALSVL
                     NKIVLMASMLFGRLEFYSILIVFLPTFWQRAH"
     gene            5908..6684
                     /locus_tag="PLI55_03115"
     CDS             5908..6684
                     /locus_tag="PLI55_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPA99859.1"
                     /translation="MAKIITVANQKGGVGKTTTAVNVAAGLAVSEIPTLLIDMDPQSN
                     ATIGCGIDYRNLENTVYEVLIEGFDINTAIMPSEIPYLDILPASTRLVGAEIEMVSLI
                     GRELVLKKSLELLTRDYKYVIIDCPPSLGLLTINSLAASDSVLIPIQCEYYAMEGLGQ
                     LLNTIRLVQRSLNRNLQIEGVLITMYDSRLRLSQEVSEQVRSHFKGKVYKTVIRRNVR
                     LGESPSFGKPIILYDATSAGAQDYMNLVGELLNDDKQTAR"
     gene            6659..7531
                     /locus_tag="PLI55_03120"
     CDS             6659..7531
                     /locus_tag="PLI55_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPA99860.1"
                     /translation="MTTSKRLGKGISALIPEFPEGIEQVYTMAEIEVAQIKFNPHQPR
                     QEFGAQAMAELIMSIREKGVIQPITVRQVDGGFELIAGERRLRACQELGKTTIPAYIM
                     PVATEAEMLELALIENVQREDLNPVDEARAYQILASTFNLSHEEIARKVGKERPTITN
                     SLRLLNLPDAILADLRSGELSAGHARPLLSIINPQQQINLWQKIKREGLSVREVEKLS
                     KKFQNIPLKEKPPLPVKTPTTKAIEDRLIHILGTKVKIKGDENKGQITIEFYSREDLA
                     RLLEIFKIIEEKTL"
     gene            7528..8337
                     /locus_tag="PLI55_03125"
     CDS             7528..8337
                     /locus_tag="PLI55_03125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPA99861.1"
                     /translation="MKRKYTLHLLSESRAKPIIWTFSHTTARLIIIGSLAFLALLIVT
                     ILITLPKAIKYRQVTLENAQLVQDRLKVSQILSDYNEIRQMDQYIRSVLGTDLVLTPS
                     DSVSGEAVNYLKNDPDQNRLVQISFLENIPIYPPVDGYITRGFIDDKVMTSENHYGVD
                     VAAAEGTPIKAAAAGVVVFSNWTYDLGYTVILYHSDGYFSIYGHNQRNSVVAHQYVKR
                     GEVIGYLGNTGKSQGPHLHFEIWREGVPINPQELIYSYKKADISLKYEGEY"
     gene            8339..8752
                     /locus_tag="PLI55_03130"
     CDS             8339..8752
                     /locus_tag="PLI55_03130"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPA99862.1"
                     /translation="MSAIIKKKESSINTIIGEKTIITGSLRLEGDIIIYGKISGDIET
                     DGTITIFPGAEIKGNLQSANLNIGGHVEGDVIATGKVTLGDRSFLRGDIKAAQIVIED
                     GAVFEGHCEMHFPYPLEAQSPNAAKPAADDANLPQ"
     gene            8829..9017
                     /locus_tag="PLI55_03135"
     CDS             8829..9017
                     /locus_tag="PLI55_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99863.1"
                     /translation="MNHKLLYEIFRSDQCQFNAYQVGRLGLSDDHQPAFSDDYVLDIF
                     SVITIKSYRFSRNHFSAG"
     gene            9077..10849
                     /gene="aspS"
                     /locus_tag="PLI55_03140"
     CDS             9077..10849
                     /gene="aspS"
                     /locus_tag="PLI55_03140"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013681444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPA99864.1"
                     /translation="MLKRTLTCGDPRVEHVEQIVILNGWVNRRRDHGGLIFVDLRDRY
                     GLTQVVFDPQSGEEIMTLARKLGLEDVIAVRGKIRRRPENAINREMATGAVELLAIEL
                     EILNEARPQPFLVTDRKTGLEDLRLKYRYLDLRTSELQRNIAIRHKTYQSVRNFYYEH
                     NFYEIETPFLTRSTPEGARDFLVPSRLCQGRFYALPQSPQQYKQILMVSGFDRYFQIV
                     RCFRDEDLRADRQPEFTQIDVEMSFVEEEDVLQSTEEMLIRVFREVAGINLESPFPRI
                     PYDEAMGQYGSDKPDLRFGYEIHTLDKIVERGEFEVFNKALASGGTVAGICFPQGASI
                     TRKLIDELTEQVKKMGVQGLAFTRVTANGLEGGIARFLTPVAREIIAEFDANDGDIIF
                     FSADSREKTYSALGVLRLEIAKRLQLIPANVFKPVWVTRFPLLEWSAEENRLVAMHHP
                     FTAAVPEEAELLATNPAQVHARAYDIVINGHEIGGGSIRNHTVEMQKRMFDVLQINPE
                     TAQEKFGFLLEALSYGAPPHGGIALGFDRLVMVLASAENLREVIAFPKTTSGASLMDG
                     SPAPVEEQQLKELGLKLIKGKSSF"
     gene            complement(10884..>11372)
                     /locus_tag="PLI55_03145"
     CDS             complement(10884..>11372)
                     /locus_tag="PLI55_03145"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HPA99865.1"
                     /translation="NKGVWLVKQNNIIEKYGYKDDRGRQALYNIVNKKTPNSQGFYLI
                     SDPERHLIILKNIYEDVNIAEWSTYVIAGKFMTKLDRLLLIIADNKIENGKEYFHFNE
                     AYLLENPTPERFLLAFEKNELMIDLRMHLKPSGGVRNHGTGFRISEKNLVDLYEKKKR
                     LI"
     assembly_gap    11373..11472
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11473..11688)
                     /locus_tag="PLI55_03150"
     CDS             complement(<11473..11688)
                     /locus_tag="PLI55_03150"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HPA99866.1"
                     /translation="MQLKELIKKLEALEQKGYLETSRQGPTGIGHLLEKEMGLFENNI
                     PIPDIGGRVELKATRRNVNSLITLFTFN"
     gene            complement(11690..13018)
                     /locus_tag="PLI55_03155"
     CDS             complement(11690..13018)
                     /locus_tag="PLI55_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPA99867.1"
                     /translation="MENINKNKQVVKIVNEPDLEYQIKIYSRNAKIVDDSWDFRNSNT
                     KDFTHCFHSYPAMMIPQVARRIIDNLGKKSKILFDPYCGTGTSLVEANLKGIDAIGTD
                     LNPLARLIATAKTTKIDIKILDLFLQDFKDYIFSIDFDIGKIYSEIIPDINNIDYWFS
                     KVTQQKLSLILKYIDNISEFSIKNFFKVAFSETVRESSYVKPGEFKLVRSKNYLDKSE
                     PDVFRMMLSKLLRNRIGLIEFIECCKNNAKTSVYDFNTINEIPNAILPTEIVDIVVTS
                     PPYGDSRTTVAYGQYSRLANEWLGFKDANKIDRILMGGEKKKHIPVFKSGILNDVIDT
                     IQKEDKERALDVISFYEDYEKSIKNVSATIKKGGFACYVVGNRTVKGLTIPNDEITAQ
                     LFEENNFSHIETIVRNIPNKRMPLKNSPSNVVGRTASTMKNEYIVIMAKN"
     gene            complement(13362..14471)
                     /locus_tag="PLI55_03160"
     CDS             complement(13362..14471)
                     /locus_tag="PLI55_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPA99868.1"
                     /translation="MNTIEKALMHGLFVHLHHNIDKVIDFKSFQELNRQVAKVWPGAL
                     LIGPEANDDAAVFQIPGMPGYVVGKMESHCSPCVPRPYDAAATGAGGAMRDVVAMGAR
                     PIFLMDFIGTRPLEQEVIVGPCGFSGRCTCGKCKVMTSQERVNIMVKGLGDMCRTMDV
                     FIIGGGFSTSFSDIVPAVVVSVIGKLVTEKPLTKPAKNVGDKVIIIGVTGNDGNDTLF
                     RAGLVEEMRPAIALFKEEKTAMEAALAAFATGKINACSDLGAAGIGAAICESARFGRR
                     GAKVDLAKVPVSAKDITPQEILICETQARYVVQVAPESVDEVLKAIRTKTEHVAVIGE
                     ITDTQQEVFEYKGEIIATIPNQPSPETLAILKSGK"
     gene            complement(14554..15009)
                     /locus_tag="PLI55_03165"
     CDS             complement(14554..15009)
                     /locus_tag="PLI55_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPA99869.1"
                     /translation="MKLSNLIKREAIVVPIQNTEKQLVIEELLEAAAATGQILDRAKA
                     LQALLDRESLGSTGLEKGLAIPHARTNAVDGLVISLGVAPDGIDFQAADGGLSYLFFL
                     LLASDALTSTYVQALALIARLYQQDDFRREILNATTPQEIIAIIRKAEE"
     gene            complement(15045..15548)
                     /locus_tag="PLI55_03170"
     CDS             complement(15045..15548)
                     /locus_tag="PLI55_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPA99870.1"
                     /translation="MPNTQSGRLISKILLLTDGSEGCISVAKYGIILSKITGAALKAI
                     YVVNTDLLKQLERARIFVKIEEVDYESDLEDDGQDYLQDITELAASKGVHLETELLKG
                     IVHKIAIQKIEEWNADLLIMGELEPGLPRQDISHDELGLLLRKARCSVLVAKEQRGLD
                     ELFETLT"
     gene            complement(15541..16176)
                     /locus_tag="PLI55_03175"
     CDS             complement(15541..16176)
                     /locus_tag="PLI55_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPA99871.1"
                     /translation="MATRIKLVPTKSNLLKLKRDLSFAQEGYELLEQKRQVLVSQLMA
                     MVNEAIVAHEKMDTALREAYSALQNAIISDGKSTVKSMARAVRIKSDIQIYNLRVMGV
                     TIPKVEVEIEDDAPYYSAGNSSFWVDESIFRFKQILEVMGRFAELRVSLLRLAQEVRK
                     TQRRVNALEKIAIPDYQETIKFISDSLEESERGTLTSMKYIKEHLEKRKDA"
     gene            complement(16189..17631)
                     /locus_tag="PLI55_03180"
     CDS             complement(16189..17631)
                     /locus_tag="PLI55_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPA99872.1"
                     /translation="MLQIKKINELNKYNQAELLKYREYIGIQEVAGPLICIENIHNVG
                     YNELVEIRDERGEPRIGQVLEANQSAAVVQVFEGTTGLSLAGTAVRFHGTPLRIPVTK
                     EMFGRIFDGLGRPIDGGPEPLTNEFWDANGESINPTARTYPTTFIQTGISAIDGMNTL
                     VNGQKLPLFSGPGLSHDRLTAQIVRQAKIVGREVSFAIVFAAMGIKHDIAQYFIDNFR
                     DAGVLENVVMFLSLADAPSVERLITPRSALTMAEYLAFHENMQVLVVMTDMTNYCEAL
                     REISIARGEIPSRKSYPGYLYSDLASLYERAGMVDNSTGSITQVPILSMPNDDISHPI
                     PDLTGYITEGQIVLDRELFQQGIYPPIALLPSLSRLMKDGIGEGLTRADHAHLASQLF
                     AAYARVKEVRALASVIGEEELSAVDHSYLKFGLAMENKFIAQKETEDRSIYETLDLGW
                     EMLSLLPREELQRITEEEAQQYHHYQNENK"
     gene            complement(17644..19410)
                     /locus_tag="PLI55_03185"
     CDS             complement(17644..19410)
                     /locus_tag="PLI55_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013701800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPA99873.1"
                     /translation="MAIVIGKVTRVLGPVVHAIATAKVQMLEFVEVGTERLIGEVVRL
                     HGNQIIIQVYEDTTGLTPGVEVYGSGMPLSVELGPGLIGTIYDGVQRPLTEIKKVSDQ
                     YIHRGIKAPALDRNKKWSFEPAVSPGTIVSSGQIIGSVKETVSITQKTLVPPNISGKV
                     ISIAPPGEYRIENEICVIENGQIRHTLSLFQRWPVRQPRPIKNRLSTSQPIITGQRVI
                     DTLFPIAKGGTVAIPGGFGTGKTMVEHQIAKWSDADVVVYIGCGERGNEMTDVLTEFP
                     QLIDPRTGRPLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYHVAVMADSTS
                     RWAEALRELSGRMEEMPAEEGFPAYLPTRLAEFYERAGYFETLNQETGSVTIVGAISP
                     PGGDFSEPVTQHTKRFVRNFLALDRDLANARHYPAISWLDSYSDYVEMVTAWWQEKAD
                     PDWAELRQEMLDLLQKEAKLQQVVKLVGADVLPESQRLILEVCTLFKNVFLQQSAYDQ
                     IDTYSTVQKQAKMLRIIVTFYRLSADVLRRGATLYKVKRTKTFRAIMNMKYEISNDEI
                     DKLDQILANLTQELASLSRPNF"
     gene            complement(19423..20046)
                     /locus_tag="PLI55_03190"
     CDS             complement(19423..20046)
                     /locus_tag="PLI55_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HPA99874.1"
                     /translation="MPDKNATLEAIVAEIRQMSDAEIEAIRQSASREQAQIAQRARQE
                     AEKIRAGIISKAQQNAEKIRQRSAAQTNLEIKKLNLQSRLLITAEIRRQFEERLFALR
                     QSPEYAQLLKELIVEGIVGLGVDHILISAGDREHTILTDEFLRSIEASFNPPVHLELE
                     PVVLNEPGVILYSADKRRRFDNRLSRYVQRVLEKYHWEIMQKFSENN"
     gene            complement(20053..20361)
                     /locus_tag="PLI55_03195"
     CDS             complement(20053..20361)
                     /locus_tag="PLI55_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013255667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPA99875.1"
                     /translation="MKYFVIGDEETVLGFSLAGADGIVVHDAASARRALAGTMEREDI
                     GIIIITERVATMIREEVNAYFYHSPYPLIVEIPDRLGPLEDRISVRDVVQTAVGVRLE
                     "
     gene            complement(20377..20778)
                     /locus_tag="PLI55_03200"
     CDS             complement(20377..20778)
                     /locus_tag="PLI55_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPA99876.1"
                     /translation="MLKIKSFKKLLAIVITLGFVAIGGTSLLVLKAIAQTPEETATSV
                     VESTETAVEVAPNTARDSDTIKFGFIAAALAVGIGSIGAAIAVGNIGTAAMGTIGEKP
                     ELSGSALIFVGLAEGIAIYGLIVAIMILGKL"
     gene            complement(20826..22790)
                     /locus_tag="PLI55_03205"
     CDS             complement(20826..22790)
                     /locus_tag="PLI55_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPA99877.1"
                     /translation="MFFPEKMLLVNALFPDKYTPKVVRMIIHQGDLQIVDAAQTNPWI
                     KDLARTYSTEESSQSQARREKVEGLVKTLKLTDQLHNFQPLSGDWMEIDQSLETAIAE
                     IESVSQRRDSLQKEFQRLNEIKTRYDRFPGLGKALQMPGDYSYLAIETGEIPDNNLPL
                     LEEQLSATLHVILPIGRQKGMTSLVAIVLKRDAEVLQAALKASGFQPTKIEKELPSLA
                     TESIEKLEKELNDLNEQLRDAEEKFQAVVNRHRALLIGVYLRIRQEQVQGRMLHFFRR
                     TEKTCLFSGWLPDSRKEVFVKELQKATQNRAIVDLIPAESHEAVKDGSVEVPVKLNNP
                     AFFKPFEMLTSVYGLPKYQTIDPTPVFGLSFLLMFGMMFGDIGHGLVLALLGLALLLK
                     SHKDTFKSAGYLLLYAGGSSIIFGFLFGSFFGIEDLFPTIWMKPMNDINQLFKVVIYV
                     GAGMITVSIIINLINGIRSGRILDYIFDKAGLLSGILYWCGIILVSRLVLTKTNGGVG
                     LFVIYLFVACALLLFFREPILNLIRKNRRLYSSGVVTGLMHSLVEMLEIVLGFLANTV
                     SFIRVGAFGLAHAGLFIAIFALSDMVSGKLSGVASILILILGNIFIIALEGLVVTIQA
                     IRLEFYEFFNRFFRPARVKYQPIRENPNLE"
     gene            complement(22799..23830)
                     /locus_tag="PLI55_03210"
     CDS             complement(22799..23830)
                     /locus_tag="PLI55_03210"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HPA99878.1"
                     /translation="MSAVSTYGLVNARVRTRRSALLSASSYKALAAATDLPEMLNLLT
                     RYKNRLSFENLPDYSIEALERLLIQEKARQILDLKYSSRGPARDIMALMLSRIDAENV
                     KTILRAWQKSDQNVSNIIECTGLAVLPITKMLSAEDLTQFAAYINQLPFYEAFLKAAK
                     GFDRSKSLFEIEVAIDKSLYQSLWDLTALLSKTDQHIVRRMVGIEIDLKNLDWIARIR
                     KYYQSQIKVTETSLIPNGYRLPYATLRKMITTGSIEDGLEKILPGLTPITAQERDQLT
                     ALERLEQFLNQALFHEANRLFLENPLSIASTIGYYYLVHIETRNLCTLINAKYYKLAP
                     QAVLNKLVY"
     gene            complement(23830..24153)
                     /locus_tag="PLI55_03215"
     CDS             complement(23830..24153)
                     /locus_tag="PLI55_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99879.1"
                     /translation="MKEILQVIIDEEKSARERIENARLQARRDTEAAQIEAQKTIETA
                     RQLALQEAEALITTAKSEAECQHAELLNAARAQQNDIFNKYHAEIAAAVDFLFQTLPI
                     GKKVA"
     gene            24646..26076
                     /locus_tag="PLI55_03220"
     CDS             24646..26076
                     /locus_tag="PLI55_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016518438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPA99880.1"
                     /translation="MYLLIAILLIAAIYSTKITSKFGVPVLLLFLGIGMLCGSDVLNL
                     IYFDNAVLAQKVANIFLIFILFEGGFHTRREIVQSVFGPALTLATFGIALTAGILGLL
                     IHFIMGLDLLYSLLIGSIISSTDAAAVFMIMRQRAIKKRVSLTLEVESATNDPMAIML
                     TIVFIQIITGHPQNIGLFILNLIWQFGGGIVVGWLISKVGAFLFNHLKVENRGYYHVL
                     SIAVCLLAYGSAEVVKANGIIAVFFAGYWLGNTDFAYRKGVGNFIEGISTFSNIAIFL
                     LLGVLVFPKSLLSVWQEGLLITAIMILVARPLTILICTLPFNFKFREKVFLMWGGIKG
                     AVPIVLATYPAVYGIDQNHFIFNIVFFAVFLSCLLQGVTLTWGASKLRLCIPSRPKPI
                     HSIELITTRKTDVDVFEIQLSDNSKIDGLQIRDLALPPDSLIIAIIRDDQIIPPKGHT
                     VLKKGDILFVIAAQDNVERISEILSK"
BASE COUNT         7150 a         5637 c         5731 g         7587 t
ORIGIN      
        1 gcggcgcaga tgtcccattc atttttcggt ttcaggctga tatggatatc ggctttcccg
       61 ctggcgacca gtgccaattt ataagcgaca ctaccacacg gttgcagggt tttgaaaaaa
      121 tgacgatagg gttgccagag attttcagtt agctcggagc gactaacgat taaacgcgct
      181 tcacttagag tttttgtctt agtactaaaa atccggctac cgttcaggaa agcgccttgt
      241 cttttataag ccgagaaaag ttcgtcgtta attggattat ataaaactcc caaaaccggt
      301 tgcccatttt cagctaatcc gattgaaacc ataaattccg gtcgcccggc aatgaattcc
      361 tttgtgccgt ccagcggatc aacgatccaa actcgcgatt tcgttaaacg ttcggcggaa
      421 tcagcgcttt cctcagacaa ccagccgtaa tccggacaag cgctaagtaa tttttctttc
      481 aggaaggcat cggcttcagt atcggcattt gtgaccggat cgtcaagacc tttgtgggta
      541 attgtatatt cctgctgaaa gtaactccgc agtatagcac cggcggctcg ggcggctgat
      601 tcagccaaac tgacaagttc cttcatagcg aataaaggat ttgccagcta cagtttggct
      661 ttgaaaatca ggatgtcctt caactgctcg ggtgtcatca cgttgcccga acggtcgagg
      721 ctattgattt ggctgttagc aacgtagtaa aaatacttgc cgtcaattgc tcctccggat
      781 ggaatgttga aatcgggatt attggattcg atgattttgt aatcggtgac actgctaaaa
      841 tccggactta attcgaaacg cacgatgcga ttcaagaagg tttgaatagc gattaaggag
      901 ttgttataaa aatttaggcc gtcacaacct accagcaaaa tatggtctgg actggttagc
      961 ggtgtgattt tgccggtggc gacatcaatg ttcagaatac cgccgccata cgttacgaat
     1021 agatgtttat cgtcgggcgt aatggttaag ctagccaatt gcggatattt atacaattcg
     1081 aggaattttt cgagagtatc cttttgggca tcgagacgga aaatagtccg ctggccggaa
     1141 tcggcaatat aaagattgcc agcccggtct actgtcaaat cagtcagaaa atgccgatta
     1201 ggcctgggca aattgtattt cttaaggagt ttgccgtcat ttaggttgta tttgtaaacg
     1261 ctggcggttc cagccaattc ccggggcgtt ttttcattgg ggattccaaa tgaacttagc
     1321 gcccacaaaa tccgacgctg gggatcagcc tgaatagcgg ttaccggacc aagtccatcc
     1381 tgccgttcag gaacaaaatc gctaatttta ccattgcggt caatttggac aattttcgat
     1441 ttataaagac tgcttatata taggtttttg gtttgtgagt cgtaggtgat gccgcgcgga
     1501 atgaggtctt tttcggcaac ggtaaaggct tttttactcg attttaccgg ctttttaacc
     1561 ttttcaattt tcttgaccag agcgcggaat tccggtgtat ttttcaaatc ggcaaaatcg
     1621 ggatcgtctt ctgctaccaa ccctaagcct ttatctacta atgaattcag gattttgata
     1681 gcattgacag tgtccttatt taaagcatag gcactggcga gattatactg tagggtaaaa
     1741 tcgtctggat aaacctgaat aagtttctga gtatattcaa gatagcgatc atattgctgt
     1801 tcactgtaag cttgaatact catattgaag taaatcccaa cgctatccgg tcgaatcgcg
     1861 ggcggattgg attgggcaaa aactgcggaa gccaggatta acaagattaa acctttttta
     1921 aacataatta actactttcc tttatttaaa ataactactt ggcaattggc aagttgggca
     1981 aatttttcgg catcagcccg actaccggag acggtacccc agtgcatcgg tacggcgacc
     2041 tgcgggtgga ttattttggt ggcttgggcg gcttcgatgg catccatcgt ataagtccca
     2101 ccaatcggca gaagagctac atcggcgcga atatcttgca tctcgggaat caagtcggta
     2161 tctcctgcat gataataaat aatgttgtcg gcgtgtatta tgtaaccgac gaactgattt
     2221 tcgcgggggt gaaatttttt accaatgtta taggcgggga cagcttttat agtaaccgaa
     2281 tcgaagttca tctgcgcacc tggtcggacc ggtcgggtgt cgccgctaat ttgtcggcaa
     2341 acactggcag gcgcaacaaa tatggtgtcg tttttcttaa tattcgtaat tgaatttcta
     2401 tcaaaatgat cgtagtgatc gtgcgtaacg aggataaggt cagccggtat cccggcttta
     2461 acctgaaagg gatcaatata gattatcttt tttccggcaa tccggaaaga tgaatgtcct
     2521 agccaagtta gattgtccag taaattcata aatcacctcc aaaagtatta ccaattcggt
     2581 actgttccga atgaccagat ataacttaga cagatcactt cgcctggagt tttggctttc
     2641 tcaaaaagac ggagggtatc tcgcagaatc gccgtatgca aggtaacatg attggctttg
     2701 ccaaacgggc gaccaaaagg aaaattgata aaagcacagc ggggaggcgc caaggctaaa
     2761 gcctgttcgt agaaggtggt cagtataact gtcggcagtt ctttcttttc caaataatta
     2821 gcaacctggc aagcggcggc cgatgtctga gctgaacaag gcagaattaa gacgccatca
     2881 acctcggctt ttttcagggt atcgtaagtt ttggtcagga atttcccgaa catttttatg
     2941 ctcttggtaa aaccggcgat actgatgatt gtctcatgta ttttgcctat cataccctct
     3001 ttttgtaaaa gcacgagacg gtcaatcggc aagacgacat tataatcagc cagagcttcg
     3061 ctgggttccc aatcagtggc gaaaaaacgc aggtcttcgc gattaaaatt gagaggaatc
     3121 tcgcggcatt ggtagtcgct ctcagtctcg cgattagtaa atggcttctg gccgttgaga
     3181 taagcgccag caatcgaaat tacggcaatt ttactttcga ttaatcgttt ctcaaaaggg
     3241 gcccacggcg tatttttctg ccgttcttga gttcgataac tacgcgccat ctgttcgtaa
     3301 agcagttgtt tacgctcagg taatggggaa aagtctgttt ttctcatagt ggtaatataa
     3361 gaaattttat gttaatctca agtcgtattt aagactttta agacgataaa acattcgatg
     3421 ctaccggact cttcaattgc tggattgttt caaaacagag attgcgcagt tcatcgggcg
     3481 aaaggtgttg gtaggtagag tagcgaatcg gctcaccgaa attcaggctg acattggcgg
     3541 aacgtaagcg ccaatcgcca cgagttttag cccgccagag cccgttaata gccagcggca
     3601 aaatgtctgc cttagcgcga tatgccagat aaaaagcacc ttttttaaag gaaagcaatt
     3661 taccatcggt agtgcggcta ccttcgggaa aaatcagaat cgaggtgcca ttcttaagca
     3721 aggtagcggc ttggtctaaa cttgttagtg aagatttgag ctgaccgtgt tggagcggga
     3781 tattccccca attacggatc atccagcccc agagtggcca cttgaaatgg tcggctaact
     3841 cgatagtagt aaaatacatc gggatataag ccgggcagat gaaagcatcc aggagactct
     3901 catgattaca aataatcaag taagattgct gatgatcaaa gcgatccaaa ccactgactt
     3961 ttatgcggat gccgatcgtt cgtagagcgg cgcggcagat ttgctgtacc agaacataga
     4021 tttcggcagg tttccaaaaa ataagcccga accggagagt cattcctaac aatagaaagt
     4081 aaaccaaccc gaccagccaa cgcccgacgt aaatcagatt ttgcatttct gagtgataaa
     4141 aacatcttga ttcatagacc aaatctggta attttcaata ctttttacaa cgttattttt
     4201 atctattcgc tgataaaata aattaggaaa tttgcaatct aatcacaaaa gaggtaaaat
     4261 atcgggcatt atgatctcta tgttgaaatg ctcataatta gggagcagtt acagttatga
     4321 aaattcgcaa cattatcgcg gttctgggta gtctttttac catattggga ttgttcctga
     4381 ttttgccgtt gctgattagc ctattccaac atgacgcggc cactccggca tttcttttca
     4441 gtattgcagt cgctacgggc gtcggattat taatgcagat tcgcaagccc cgcaatttta
     4501 atattcagcg ctgggaggca atggtaatcg tggttatcgg atggatcggg gcatctgtga
     4561 tcggcgcatt gccgttttat ttcagcggat atttcgatgg ttttataaac gctctgtttg
     4621 agtcggtttc gggattcacc accaccggcg cttcgattct aaatgatatt gaaagcctac
     4681 cgattagtct gcttgtctgg cgcactctga cgcactggat cggtggtatg ggaatcataa
     4741 tcctggcgtt agtagtgctt ccagctatta atattagcgg acgcaatttg tatagcttcg
     4801 aatcctcttc gcttgccaat gaacaaatca cgcctaaaat caaagatacc gcgcgtttgt
     4861 tatggacggt gtattttatc ctgacagtct ccgaaaccgt tcttttatta gttttaggtc
     4921 tgcccttttt cgatgcggca ttaacggcta tggcgactat cgccacgggc gggttttcac
     4981 ccaaaaacgc cagcatcgcc gcctataaca atgtatatgt tgaaatagtc gtcgttattt
     5041 ttatgatact ctccggcata aatttcactc tctacctacg aatgttccaa tcacgaaaga
     5101 actggcggga ttactcagaa acagtctata tctactttgc gattatggcg gtcgcatctt
     5161 tggtcatagt gattaatttg cgttcgcata cttacaattc ttggtcagaa gcaattcgct
     5221 atggagtatt tcaggcagtt tcaattaata cgacgactgg attcgttaca actgattggg
     5281 aaatctggca accggtgaca catattgttt tgttaatttt aatgctgatc ggggcttgcc
     5341 cgggatcgac gtgcggagcc ataaaaaatt cgcgaatcat tctgcttatc aaaagcatcc
     5401 atcgtgagct cattcagata atccatccac gggtagtcac gactattcat tttaataaac
     5461 gcccaattga tgaaaaaaca atccagaacg caattgtgta tattggaatg tatctgtgtt
     5521 tttggttggt cgcgagtatg atcgtggctt taggcggcgt tgccccggaa tcagcgctca
     5581 ttgccgtggc tacaaccctg ggaggcgttg gaccaggcat tggtttattt ggtccgatag
     5641 aaagttttgc cgctctgtcc gttctgaata aaatagtatt aatggctagt atgttatttg
     5701 ggcgattgga attttattca attttaattg tattccttcc tactttctgg caacgggctc
     5761 attaagtcag tgttgtggga agacttactc tctgctataa ggcttttaac ttgtttttta
     5821 gagtaggcag ggtttaaatt tgcttggttt tttaataata tttaatactt gaaatatatt
     5881 gaaattaaag aaattaatag aagctagatg gccaaaataa tcacggttgc taatcaaaaa
     5941 ggtggtgtcg gaaaaaccac aacggcggtt aacgttgccg ccggactggc ggtttccgaa
     6001 attcctactt tgctgattga tatggatccg caatcgaacg ccacgatcgg atgcggcata
     6061 gattatcgca atttggaaaa tacggtttat gaggtattaa ttgaaggctt tgatataaat
     6121 acggcgatta tgccttccga aattccttat cttgatattt tgccagcttc cacccgttta
     6181 gtaggcgctg aaattgagat ggtttcacta atcggccgag aattagtttt aaagaaatct
     6241 ctcgaattgc taacccgtga ttataagtat gtaataattg attgcccacc ttcattggga
     6301 ctcttgacga tcaatagtct agccgcttca gattcggtgc taattccaat tcagtgcgaa
     6361 tattatgcga tggaggggct cgggcaatta ctaaatacga tccgcctggt ccagcgtagt
     6421 ttaaaccgca acttacagat cgagggcgtg ctgattacga tgtatgatag tcgcttacgt
     6481 ctctcccagg aagtttcgga acaggtgcgc agtcatttca aaggaaaggt ttacaaaact
     6541 gtaatccggc gcaatgtccg cctcggtgaa tcccccagct ttggtaagcc gattatcctt
     6601 tatgatgcta cctcggctgg cgctcaggac tatatgaatc tggtaggaga actactcaat
     6661 gacgacaagc aaacggctag gtaaaggtat tagcgcacta atccctgaat ttccggaagg
     6721 aatagaacag gtttatacga tggctgaaat cgaagtggct caaatcaagt tcaatcccca
     6781 ccagccgcgc caggaatttg gggctcaggc gatggctgaa ctgattatgt caattcggga
     6841 aaaaggagtg attcagccga tcaccgtgcg ccaggtcgat ggcggctttg agttaatcgc
     6901 cggtgagcgt cgtctgcgag cttgtcaaga actcggtaag acgaccatac cggcttatat
     6961 tatgccggta gccactgagg ctgaaatgct ggaattggct ttaattgaaa atgtccagcg
     7021 tgaagacctt aatccggttg atgaagctcg cgcttaccaa attttagcca gcacattcaa
     7081 tctatcccac gaagaaatcg ctcgaaaagt tggcaaagaa cgaccgacga tcactaattc
     7141 cttacgcctg ctcaacctac cggatgccat tctggctgat ttaaggtctg gcgaactcag
     7201 cgccggccat gcccgaccgc ttctctccat tataaatcca caacaacaaa ttaatctctg
     7261 gcaaaaaata aagcgagaag gtttgagcgt tcgggaagtc gaaaaactat ctaagaaatt
     7321 tcaaaatatt cctctcaaag aaaagccacc gctaccagtc aaaaccccta ctaccaaagc
     7381 cattgaggat agattgattc acattctcgg taccaaggta aaaattaagg gagacgaaaa
     7441 caaaggccag atcacgattg aattttattc tcgcgaagac ctggcacgtc tgctggaaat
     7501 attcaaaatc atcgaggaaa aaacattatg aagagaaaat ataccttaca cttactttcg
     7561 gagagccgcg caaaacctat tatctggact tttagtcaca cgactgcccg gttgattatt
     7621 atcggttcgc tagcttttct agctctattg atagtcacta ttctgattac cttacccaaa
     7681 gcaatcaaat accggcaggt tactcttgaa aacgcccaac tggttcagga ccgtctcaaa
     7741 gtatctcaaa tactgagtga ttacaatgag attcgccaga tggaccaata tattcgttcg
     7801 gtattgggca ccgatctcgt cctgactccc agcgattctg tctccggtga agccgtcaat
     7861 tacctaaaga atgatcccga tcaaaaccgg ttagttcaga tcagtttttt agaaaacatt
     7921 ccgatttacc cgccagtgga tggttatatt acgcgcgggt tcattgatga taaagtaatg
     7981 accagcgaga atcactatgg tgtagatgtt gccgccgccg aaggaactcc cattaaagcc
     8041 gccgccgccg gggtagtggt tttttctaac tggacttatg atctcggcta tactgtcatc
     8101 ctctatcatt ccgatggata ttttagcatc tatggccata atcagcgtaa tagtgtagtc
     8161 gctcatcaat atgtgaaacg cggcgaggta atcggctatc ttggcaacac cggaaaaagt
     8221 cagggaccac acctgcattt tgaaatctgg cgcgaaggtg ttccgatcaa tccccaggaa
     8281 ttgatttatt cctataaaaa agccgatatt tcccttaagt atgaaggaga atattaatat
     8341 gtctgccata atcaagaaaa aagaatcttc catcaatact attatcgggg agaaaaccat
     8401 cattaccggg agcttacggc tggaaggcga tataatcatc tacgggaaaa ttagcggtga
     8461 tatcgaaacc gatggcacca tcacaatttt ccccggagct gaaatcaaag gcaaccttca
     8521 aagcgctaac cttaatatcg gcgggcatgt ggaaggggat gtcatagcta ccggaaaagt
     8581 aaccctcggc gaccgctctt ttttacgcgg cgatatcaaa gccgctcaga tcgtgatcga
     8641 agacggcgcg gttttcgaag gacactgtga aatgcatttc ccttatcccc tggaagccca
     8701 atcgccaaat gcggcaaagc cggcagcgga tgacgcgaat ctgccgcaat aaaaattacc
     8761 gcattaattg accccaattt gctattttgg gacaactgta tatttttccc tattaaaaac
     8821 gttaattatt gaaccacaaa ttactctatg aaattttccg gtctgatcaa tgccaattta
     8881 atgcctatca ggtgggacga ctgggattat ccgatgatca tcaaccagct ttttctgatg
     8941 attacgttct ggatattttt tcggtgatta ctattaagag ctaccggttt agtcgaaatc
     9001 atttttctgc tggttaaaac tatatggaat cattaatttt gcccgtttga aagattattt
     9061 aaataatgga taagcaatgt taaaacgtac tctgacctgc ggcgaccccc gcgtcgaaca
     9121 cgttgaacaa atcgtgattc tcaatggctg ggtcaaccgt cggcgcgatc atggcggatt
     9181 aatctttgtc gatttacgcg accgttatgg tctaacgcaa gtggtatttg atccccaatc
     9241 gggtgaagaa ataatgactt tggctcgtaa actcggtttg gaagatgtaa ttgccgtgcg
     9301 aggcaagatt agacgccgtc cggaaaatgc catcaaccgc gaaatggcta cgggtgccgt
     9361 cgaactactg gctatcgagc tggaaatttt aaatgaagca cgccctcagc ctttcctggt
     9421 aaccgatcgc aaaacagggc tggaagattt gcgcttgaaa tatcgctatc tcgatttacg
     9481 gacgagcgag ctccagcgca atattgccat ccggcataag acttatcaaa gcgtgcgaaa
     9541 tttttattat gaacataatt tctatgagat agaaactcct tttttgactc gtagtactcc
     9601 cgaaggagcg cgtgatttcc tcgtgcccag tcgcctatgt caaggtcggt tctatgctct
     9661 accgcagtcg cctcagcaat ataagcagat tctaatggtg tctggcttcg atcgctattt
     9721 tcagattgtc cgctgtttcc gggatgagga tttgcgcgcc gaccgccaac cggaatttac
     9781 tcagattgac gtcgagatgt catttgtgga ggaagaggac gtattacagt caaccgaaga
     9841 aatgctaatc cgcgttttcc gcgaagtggc aggaattaat cttgaatcgc ctttcccccg
     9901 cattccctat gatgaggcta tggggcaata cgggagcgat aaaccggacc tgcgtttcgg
     9961 ttatgaaatt catactttgg ataaaattgt cgagcgcggt gaattcgaag tttttaacaa
    10021 agcattggct tcgggaggaa ccgttgccgg aatttgtttc cctcaagggg cttcgattac
    10081 tcgcaaacta attgatgaat taaccgagca ggttaagaaa atgggagttc aaggactggc
    10141 ttttaccaga gtaacggcaa acggtttaga aggtggaatt gctcgtttct tgacgccggt
    10201 cgcccgcgaa attatagctg agttcgatgc taatgacggt gatataattt ttttcagtgc
    10261 cgattctcgg gaaaaaacct attcggcgct cggcgtcctg cggctggaaa ttgccaaacg
    10321 cttgcaattg attccggcga atgttttcaa gccggtttgg gtgacacgct tccccttact
    10381 ggaatggagc gcagaagaga atcgtctggt cgccatgcat catcccttca ctgccgctgt
    10441 gccggaagaa gccgaattat tagcgactaa tccggcgcaa gtacacgcac gggcatacga
    10501 cattgtgatc aatggccacg aaatcggcgg cggtagtatc cgcaatcata ctgttgaaat
    10561 gcagaagcgg atgttcgatg tcttgcagat caatcccgaa acagcgcagg aaaaattcgg
    10621 ttttttactg gaggcactga gctacggcgc gccgccgcac ggcggaattg ctctaggttt
    10681 tgatcgcctg gttatggtat tggcgagtgc cgaaaatttg cgggaagtga ttgcttttcc
    10741 gaaaactacc agcggcgctt ccctgatgga tggtagccca gctccggtag aagaacaaca
    10801 gttgaaagaa ctcggcttaa aactgattaa gggtaaatcc tctttttaaa tatacttttt
    10861 tagcgatgtt tttgtaaaaa aatctagatc aatctttttt tcttttcata caaatcaaca
    10921 agatttttct ctgaaattcg gaatccagtc ccatgatttc tgacgccacc ggatggcttc
    10981 aaatgcattc gtaaatcaat catcagttca tttttttcaa aagctaataa aaatctttct
    11041 ggagttggat tttccagcag ataagcctca ttaaagtgga agtattcttt tccattttct
    11101 attttattgt cggcaattat taaaagtaat ctatcaagct tggtcatgaa tttaccagca
    11161 attacatagg tgctccactc cgcaatattc acatcttcat agatgttttt taagatgatc
    11221 aagtgccttt caggatctga aatgaggtaa aatccctggg aattcggcgt ttttttatta
    11281 acaatgttat atagtgcttg tctacctcta tcatctttgt atccatattt ttcgataata
    11341 ttgttttgtt tgacaagcca aaccccctta ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnnn nnattaaaag taaaaagtgt tattagcgag tttacattcc gtctagtagc
    11521 ttttagttca actctacccc caatatctgg aataggaata ttgttttcaa ataatcccat
    11581 ttctttttct agtagatgcc caataccagt tggtccttgt cttgaagttt caagatatcc
    11641 tttctgctca agggcttcta atttttttat tagttctttt aactgcatat caattttttg
    11701 ccataataac tatatattca tttttcattg ttgaagcggt cctgccaacg acattagaag
    11761 ggctattttt taaaggcatc cttttattag gtatattcct aacaatagtt tcaatatgac
    11821 tgaaattatt ttcttcaaat aactgagctg ttatttcatc gttaggaata gtaagacctt
    11881 taactgttcg gttgcccaca acataacaag caaaaccacc cttttttatg gtagctgaaa
    11941 catttttaat tgatttctca taatcttcat aaaaggaaat tacatcaaga gctctttctt
    12001 tatcttcttt ctgaattgta tctattacat cgtttaatat tccactttta aatacgggaa
    12061 tatgcttctt cttctcccca cccattaaaa ttctatctat tttatttgca tctttaaaac
    12121 caagccattc atttgctaac ctagagtatt gcccataggc aacggtagtg cgtgaatcgc
    12181 cgtagggagg agatgtcaca acaatatcta caatctctgt tggaagaata gcatttggaa
    12241 tttcatttat tgtattgaaa tcatataccg acgtcttagc gttattttta caacactcta
    12301 taaattcgat aagtccaatt ctatttctta ataattttga aagcatcatt ctaaaaacgt
    12361 caggttcaga tttatctaaa taatttttgc tcctgactaa tttgaactct cccggtttta
    12421 cgtatgaaga ctcgcgaact gtttcagaga aagcaacttt aaaaaagttt ttaattgaaa
    12481 attcgctaat attatcaatg tattttagta tcaatgatag tttttgctga gtaacttttg
    12541 agaaccaata atctatattg tttatatctg gaataatttc cgaatatatt ttgccaatat
    12601 caaagtctat agaaaaaatg taatccttaa aatcctgaag aaataagtca agtattttga
    12661 tatcgatctt tgtagtttta gccgtggcga ttaacctcgc caaaggattc aaatcagtac
    12721 cgatagcatc tatacctttt agattagctt caactaaaga cgtccctgtc ccacaataag
    12781 gatcgaataa gatttttgat ttcttaccca aattatctat aatccttcta gcaacttggg
    12841 gaatcatcat tgctggatat gagtgaaaac aatgcgtaaa atctttggta tttgaatttc
    12901 taaaatccca actatcatcc acaatctttg catttcgaga atatattttt atttggtatt
    12961 ctaaatcagg ttcatttaca atttttacta cctgtttatt tttattaata ttttccattg
    13021 tattagagct agtgagacct catatttatt acaaactacc tattgcacaa aattttgatc
    13081 atttgctaaa aattaagtca tcaaaggtaa tttgccaacg acaattaaat atcaagctat
    13141 ttattatttt taggatattt agggtatctt gtggggggat ctgattttgt tttctgtaat
    13201 taccacatag ccatgtctaa tattcagaga tttttgtacc ttttaagata aactcataat
    13261 ccaaatattt atattgacat tccgagatga aaattcaaga ttgccactct caccttctta
    13321 aagccggtaa ctgtttatct aaatttctta tcgggtttta tttatttgcc acttttcaga
    13381 attgctaaag tttcgggcga tggttggttg ggaatcgtgg caataatctc acctttgtat
    13441 tcgaaaactt cctgctgggt atcggtgatt tctccgatta cggcgacgtg ctcggttttg
    13501 gtgcgaatgg cttttagcac ttcatctact gattccggcg caacctgaac gacatagcgt
    13561 gcctgggtct cgcaaattaa tatttcctgc ggagtaatat ctttggcaga aactgggact
    13621 ttagccagat caactttggc accgcgccga ccgaaacggg ctgattcgca aattgccgca
    13681 ccaataccag cggcaccgag atcggaacag gcattaatct tgccagtcgc aaaagccgcc
    13741 aaggcggctt ccatcgcggt tttttcttct ttaaataggg caatggccgg gcgcatttct
    13801 tctaccaatc ccgcccggaa aagggtgtcg ttgccgtcat tgccggtcac gccaatgatg
    13861 ataactttat caccgacatt tttggctggt ttggtaagtg gtttttcggt aaccagtttg
    13921 ccgataaccg aaacaacgac agccggtacg atatcggaga aagaagtaga gaaacctccg
    13981 ccaatgataa aaacatccat cgttcggcac atatccccca aacctttgac cataatattc
    14041 actctttcct gactggtcat aaccttacat ttgccacagg tgcaacgccc cgaaaaaccg
    14101 catggcccaa ctatgacttc ctgctccagt ggtcgcgtgc cgataaaatc catcaggaaa
    14161 ataggacgag cgcccattgc gaccacatca cgcatcgcac caccggcacc ggtggcggcg
    14221 gcatcatacg gccgtggcac acagggcgaa cagtggcttt ccattttacc aactacgtaa
    14281 cccggcatac ccgggatttg aaaaactgcg gcatcgtcgt tagcttccgg gccgatcaat
    14341 aatgctcctg gccaaacttt agcgacctgg cggtttaatt cctgaaagct tttaaaatca
    14401 attactttat caatattgtg atgtagatga acgaatagtc catgcatcaa ggctttttct
    14461 attgtgttca ttgtttctcc tttttaatgt tatctgttat cggtttttaa tagtgatgat
    14521 attgtcgctc aaattaaggt gcgaagccac acgttattct tcggctttac gaataatggc
    14581 gataatctct tgaggagtgg tagcattcaa tatttcacga cgaaagtcat cctgttgata
    14641 aaggcgtgcg atcaatgcaa gagcctgaac ataggttgag gtcaatgcat ccgaagccag
    14701 caatagaaag aaaaggtaag aaagaccgcc gtcagcggct tgaaaatcta tgccatccgg
    14761 ggcaacgccc agtgatatta ctagaccgtc aaccgcatta gtgcgggcat gcgggatcgc
    14821 cagtcctttt tcgaggccgg tactgcctaa gctttcacga tccaaaaggg cttgtaaagc
    14881 ttttgcgcgg tcaagaatct gacctgtggc cgcggcggct tctagtagtt cctcgattac
    14941 taattgtttt tcggtattct gaattggtac gacaatcgcc tcgcgcttaa tcagattgga
    15001 aagtttcatt ttaaaaaaag ctcctcgttt tactttgaca aaattcaggt taaagtttca
    15061 aataattcgt caagacccct ctgctcttta gctacgagca ccgaacagcg cgctttacgt
    15121 aaaagcaagc cgagttcgtc gtgagagatg tcctgacggg gcagacctgg ctcaagttca
    15181 cccattatca ggaggtcagc gttccattct tcgatcttct ggattgcgat tttatggacg
    15241 atgcctttca ataattcggt ttcaagatga acgcccttgg aagcggccaa ttctgtgatg
    15301 tcttgcagat aatcttgacc gtcatcttct agatcacttt cgtaatcgac ttcttcgatt
    15361 tttacaaaga tacgagcgcg ctcgagctgc ttgagtaaat cagtgttgac gacataaatt
    15421 gctttgagag ccgctccagt aatttttgaa aggatgatgc catacttggc aaccgaaata
    15481 catccctctg aaccatcagt cagcaggaga atttttgaaa tgagacgtcc cgattgcgta
    15541 ttaggcatcc tttcgttttt caaggtgttc tttgatgtat ttcatcgaag taagtgttcc
    15601 acgctcgctt tcttccaaag aatctgaaat aaatttaata gtttcttggt aatcggggat
    15661 agcaattttt tccagggcat tgactcgccg ctgggtcttg cggacttcct gagccaggcg
    15721 taaaagcgaa acacgcaact cagcaaagcg tcccatcacc tcgagaattt gtttaaatcg
    15781 gaaaatactt tcgtcaaccc agaatgacga attaccggca ctatagtaag gcgcatcatc
    15841 ctctatttca acctcgactt tcgggattgt cacacccatt acccgtagat tgtaaatctg
    15901 gatatccgat ttgatccgaa ctgctcgcgc catagacttg accgtgcttt ttccatcgga
    15961 aataatggca ttttgaagag cactatacgc ttcgcgcaaa gccgtatcca ttttttcgtg
    16021 cgccacaatc gcttcattaa ccattgccat cagttgtgaa accagcacct ggcgcttctg
    16081 ctctaaaagt tcatagcctt cctgggcaaa gctcaagtcg cgctttaatt tgagcagatt
    16141 acttttggtc gggacgagtt tgatgcgggt tgccatttta ctaattattt atttattttc
    16201 attttggtaa tggtgatact gctgggcttc ttcttcagta atgcgttgta attcttcccg
    16261 cggcaggagc gacaacatct cccaacctaa atcgagggtt tcataaatcg aacgatcttc
    16321 ggtttctttc tgagcaataa atttgttttc catagccaga ccgaatttta agtaactgtg
    16381 gtcaactgca ctgagctctt cctcaccaat gacggaagcc agagcacgca cctccttcac
    16441 ccgggcataa gccgcaaata gctggctggc taaatgcgca tgatcggcgc gggtcaaacc
    16501 ttccccgatt ccatctttca tcagacgcga aagagaaggt aaaagggcga tcggcggata
    16561 aattccctgc tggaaaagtt cgcgatccag aacgatttgt ccttcggtaa tatatcctgt
    16621 aagatcggga ataggatgcg aaatatcatc attcggcatc gaaagaatgg gaacttgagt
    16681 aatcgaaccg gttgagttat ctaccatacc ggcgcgttcg taaagcgaag ctaggtcgga
    16741 gtatagatag cctggataac ttttgcggga cgggatttca ccgcgggcaa tcgaaatttc
    16801 tcgtaatgcc tcacaatagt tggtcatatc ggtcataacc accagcactt gcatattttc
    16861 atgaaaggct aaatattcag ccatagtcaa agccgaacgc ggcgtgatca ggcgttcaac
    16921 cgacggagca tcagccaatg acaggaacat tacgacattc tccagaacgc cggcatcccg
    16981 gaaattatct atgaagtatt gagcaatgtc gtgcttgatg cccatcgcgg caaagacaat
    17041 ggcaaaggat acctcgcggc ctacaatttt agcttgacga acaatttgag ccgtcaaacg
    17101 atcgtgtgat aaacctgggc cagaaaatag cggcagtttc tgtccattga ccagagtgtt
    17161 catcccgtca atagctgaaa tccccgtctg aataaaagta gttggatatg tccgggcggt
    17221 tggatttata gattcaccgt tggcgtccca gaattcattg gtcaagggct ccggtccacc
    17281 gtcgattggg cgtcccaaac catcgaagat tcgcccgaac atttctttgg taacgggaat
    17341 tcgcagaggc gtgccatgaa accgcactgc tgttccggct aatgataaac cggtggtgcc
    17401 ttcgaaaacc tgaacaacgg cggcggattg gttggcttcc aggacctggc caatgcgtgg
    17461 ttcgccgcgt tcatcgcgga tttcgactaa ttcattatag ccaacattat ggatattttc
    17521 gatacaaatt agtggcccag caacttcttg aattccaata tattcccggt atttcaagag
    17581 ttcagcctgg ttatatttat tcaattcatt aatttttttg atttgcaaca ttttgctaaa
    17641 gccttaaaaa tttggacgac tgaggctagc caattcttgg gttagatttg ccaagatttg
    17701 atctaattta tcaatctcat cattggaaat ttcatatttc atattcataa tggcacgaaa
    17761 ggttttagtg cgtttaacct tataaagagt agctccacgt ctgaggacat cggccgataa
    17821 ccgataaaag gtaacgatga tgcggagcat tttcgcctgt ttttgaacag tggaataggt
    17881 atctatctgg tcgtaagccg attgttggag aaaaacgttt ttgaaaaggg tacagacttc
    17941 taggatgagg cgctgagatt ccggcagaac atccgcaccg actaatttga cgacctgttg
    18001 aagtttagct tctttctgga gaagatcgag catttcctgt cgcaactcag cccaatccgg
    18061 atcggctttc tcctgccacc aggcagtcac catttcgaca taatcagaat aagaatcgag
    18121 ccaggaaatc gccggataat ggcgcgcatt agctagatcg cgatcaagcg ccaggaaatt
    18181 gcggacgaag cgcttggtat gttgagtcac cggctccgag aaatcaccac cgggcggtga
    18241 aatagcgccc acgatggtaa ccgagccggt ttcctggttg agagtttcga aataaccggc
    18301 gcgctcgtaa aattcagcta agcgagttgg caaataagct gggaaacctt cttcggctgg
    18361 catttcttcc atacggccag acaattcgcg caaagcttcc gcccagcgcg aagttgagtc
    18421 agccataacg gcgacgtgat aacccatatc acgaaaatat tcggcaatgg tgatgccggt
    18481 gtaaatcgaa gcctcacgtg cggcaaccgg catattggaa gtattggcaa tcagtacggt
    18541 gcgttccatc agcggtcgcc cggtgcgcgg atcaattaat tgaggaaatt cagtcaagac
    18601 atcagtcatt tcgttgccgc gttcaccaca gccaatatag acgacgacat cggcatctga
    18661 ccacttggcg atctggtgtt caaccatagt tttaccggta ccaaatccac ccggaatagc
    18721 gactgtgcca cctttggcaa tcgggaaaag ggtatcaata acgcgctgtc cggttataat
    18781 cggttgactg gtggacaaac ggtttttaat tggtcgtggc tgacgcactg gccagcgctg
    18841 aaaaagggac aatgtatgtc ggatttgacc attttcgata acacaaattt cattttcgat
    18901 acgatattct cccggcggcg caatgctaat gaccttgccg gaaatgtttg gcggaactaa
    18961 tgttttttgg gtaatggaaa ccgtttcctt tactgacccg attatctgcc cactgctaac
    19021 gattgtaccg ggacttacag ctggttcgaa tgaccatttt ttattgcggt cgagagcagg
    19081 agctttgatg ccacgatgaa tatattgatc ggaaaccttc ttgatttccg tcagcgggcg
    19141 ttgaacaccg tcatatatag tgccaatcag accgggcccg agttccactg aaaggggcat
    19201 tccggaaccg tacacttcta ctccgggcgt taaaccggta gtatcttcat aaacttgaat
    19261 gatgatttga ttgccgtgaa gccgcacaac ttcgccgatc aggcgttcgg tcccaacctc
    19321 gacaaattcg agcatctgaa ccttagcagt tgcgattgca tgaacaactg gtcccaaaac
    19381 gcgggtgact tttccaataa ctattgccac cgtttacctc ttttaattat tttccgagaa
    19441 tttctgcata atttcccagt gatatttttc caggacacgc tgaacataac gggataagcg
    19501 gttatcgaat cgcctccgct tatctgccga ataaagaatg acccccggct cattgagaac
    19561 tacaggctcc agttcaaggt gcaccggtgg attaaaagat gcctcaattg atctaagaaa
    19621 ttcatcggtt aggatggtgt gctctcgatc gccagcgcta atgagaatgt gatcaactcc
    19681 caagccaaca attccttcca caattagctc tttcagcaat tgggcatatt caggagattg
    19741 gcgcaaagca aacaaacgtt cttcaaattg acgccggatt tcggccgtga ttaaaagtcg
    19801 tgattgcaaa ttcaattttt tgatttcaag attggtttga gccgctgatc gctggcgaat
    19861 tttctcggca ttttgctggg ctttactgat aattccggcg cgaatttttt cagcctcttg
    19921 gcgggcacgt tgagcaattt gggcttgctc gcggctggca gactgacgga tggcttcaat
    19981 ttcagcatcc gacatctgac gaatttcagc tacaatagct tccagagtgg cgtttttatc
    20041 aggcataatt cctcattcta accgaacacc aaccgcagtt tggacaacat ctcgcactga
    20101 aatgcggtct tccagtgggc caaggcgatc agggatttca acaatcaagg gataagggct
    20161 atgataaaaa taagcattga cctcttcccg aatcattgta gcaacgcgct cggtaattat
    20221 aatgatgccg atatcttcgc gctccatagt accagccaga gcgcgtcgag cggaagccgc
    20281 atcgtgaacc acaattccat ccgcacctgc taggctgaat cccagaacag tctcttcatc
    20341 accaatcacg aaatatttca caacatctcc ggcaaattac aattttccga gaatcataat
    20401 ggctacgatc aatccgtaaa tagcgatacc ttccgccaga ccgacgaaaa tcagagcgct
    20461 accggacaat tctggttttt cgccaattgt gcccatagcc gcggtaccaa tattgccaac
    20521 ggcaatggcg gctccgatcg aaccaatgcc aactgccaga gcggcggcaa taaaaccaaa
    20581 tttgatcgta tcggaatcgc gagccgtatt cggagcgacc tcgactgcgg tttcagtgct
    20641 ctctactaca ctcgtcgcgg tttcttccgg agtctgtgca atagccttca gcaccagtag
    20701 tgaagttccg ccaattgcca cgaaacccag cgtaatcaca attgccagca gttttttaaa
    20761 ggatttgatt tttaacatat ttgcattacc tccgcaaatt tatttttcat attattagag
    20821 aatatttact ccaggttggg attctctctg attggttgat acttaaccct cgccggacgg
    20881 aaaaagcgat tgaaaaattc ataaaattcc agacgaattg cctggatggt tactactagt
    20941 ccttccagag caatgatgaa aatattgcct aaaattagaa ttaagataga agctactcca
    21001 cttaatttgc cactcaccat atccgaaaga gcgaatatgg cgataaataa tccggcatgg
    21061 gctaacccaa acgctcccac acggatgaaa gaaaccgtat tagccaaaaa tcccagcaca
    21121 atctcaagca tttctacaag cgaatgcatt aatccggtga caactcccga actatataga
    21181 cgccggtttt tgcggattaa attaaggatt ggttcccgga agaaaagaag cagagcgcaa
    21241 gccacaaaga gatagatcac aaaaaggcca actccaccat tagttttcgt gagtaccaga
    21301 cgacttacca atatgatacc gcaccaatag agaatgccgc tcagtaaacc ggctttgtca
    21361 aaaatataat cgagaattct gccgctacgg atgccattta ttaaatttat aataatcgag
    21421 acggttatca tcccggcacc aacgtagatc acaactttga acaattgatt gatgtcattc
    21481 atcggtttca tccagatggt tggaaataaa tcttcgatcc cgaaaaaact accgaataga
    21541 aaaccgaaaa taatactgga accaccggcg tagagtagta aatagccggc cgatttgaat
    21601 gtatctttgt gactttttaa tagcaacgct aagccaagca atgccaagac cagaccgtgc
    21661 ccaatatctc caaacatcat tccgaacatc aaaagaaaac ttagaccaaa caccggtgtt
    21721 gggtcaatgg tctgatactt cggcagacca tagaccgaag tcagcatctc aaagggtttg
    21781 aaaaaggcgg ggttatttaa tttgaccggg acttccacac taccatcttt gactgcttca
    21841 tgcgattcag ccggtatcag gtcaacgata gcccgatttt gggtggcttt ctgcaattct
    21901 ttgacaaaaa cctccttgcg gctatccggc aaccagcccg aaaaaagaca ggtcttttca
    21961 gtacggcgga agaaatgtaa catccggcct tgaacttgtt cctgacgaat acgcaggtaa
    22021 acgccgatta ggagcgcgcg atggcgattg actaccgcct ggaatttttc ctcggcgtca
    22081 cgcagttgtt cattgagatc gttcagttct ttttcgagtt tttcaattga ttcggtggcg
    22141 agactgggca attctttttc aattttggtc ggctggaaac cgctggcttt gagcgccgcc
    22201 tgaagaactt cggcatcgcg tttgagaacg attgccacca gtgaggtcat tcctttctgt
    22261 ctcccgatcg gtagaattac atgtagagta gcggataatt gttcttccag tagcggtagg
    22321 ttattgtcgg ggatttcgcc tgtctcaatg gcaagatagg aatagtcacc cggcatttga
    22381 agggctttgc cgagaccggg aaagcgatcg tagcgggttt tgatttcatt gagacgctga
    22441 aattcctttt gcaggctgtc gcgcctctga ctaactgatt cgatttcagc aatggcagtc
    22501 tcgaggctct gatcaatttc catccaatca ccgcttagcg gttgaaagtt atggagttgg
    22561 tcggttaatt taagggtttt aactaaccct tccacttttt cccgacgggc ttgagattga
    22621 gaggattctt ccgttgaata tgttcgtgcg aggtctttga tccaaggatt agtttgggcg
    22681 gcatccacaa tttgcaaatc gccctgatgt ataatcatgc ggacaacctt gggcgtatat
    22741 ttgtcgggaa agagcgcatt gaccaaaagc attttttctg ggaaaaacat ctttatcctc
    22801 aatataccaa tttatttaaa accgcctgcg gtgctaattt ataatattta gcatttatta
    22861 atgtgcagag attccgggtt tcgatatgaa ctagatagta gtagccaatt gtggaggcga
    22921 tagaaagtgg attttccaga aaaaggcggt tagcttcatg aaaaagcgcc tgattgagaa
    22981 attgctctaa gcgttcaaga gcagtaagtt gatcacgttc ctgcgcagta atgggcgtca
    23041 atcccggtaa tattttttcc agtccgtctt cgattgagcc ggtagtaatc atttttcgga
    23101 gagtagcata aggcaaacga tagccgttgg gtattagact tgtctcggtg actttaattt
    23161 gagactggta atatttacgg atgcgggcga tccaatccag gtttttcagg tcaatttcga
    23221 tgccgaccat tcgtcgtact atatgttgat cggttttact caataaagca gtcaggtccc
    23281 aaagggactg atacaggctt ttatcaatgg caacttcaat ttcaaagagg ctcttgcttc
    23341 ggtcgaaacc tttagcggct ttcaggaaag cttcataaaa cggtagctga ttgatataag
    23401 cggcaaattg ggttaaatcc tccgcactca gcatttttgt aatcggcaga actgccaagc
    23461 cggtgcattc gataatgtta ctgacattct ggtcggattt ttgccaagcc ctgagaatag
    23521 ttttgacgtt ttcagcatca attcttgaga gcatcaaagc cataatgtcc cgagctggtc
    23581 cgcgactgga gtacttcaaa tcaagtatct gacgggcttt ctcttggatt aataaccgct
    23641 ccaaagcttc aatcgaataa tcaggaaggt tttcgaaact gagacgattt ttatagcggg
    23701 ttaagagatt aagcatttcc ggtaaatcag ttgcggcggc taaagcctta taagatgaag
    23761 cagaaagcaa ggcggagcgt cgtgtccgga cgcgagcatt taccagtccg taagtgctaa
    23821 cagcactcat taggcgacct ttttgccaat tggaagggtt tgaaaaagga aatccacagc
    23881 ggcggcgatt tcagcatggt atttattgaa aatgtcgttt tgttgcgctc gagcggcatt
    23941 taacagctcg gcgtgttggc attcagcttc ggatttggcg gtcgtaataa gcgcttcggc
    24001 ttcctgtaat gccaattggc gagcggtttc aatggttttt tgggcttcaa tctgagcggc
    24061 ttctgtatcg cggcgggctt gaagacgagc gttttctatt cgttcgcggg cagatttttc
    24121 ttcgtctatt attacctgaa ggatttcttt caagttaacc tcaagtttca gccggtttag
    24181 atatccaggc tatcattggt aagtttgtaa accggcttaa aataaatact attcaagctg
    24241 acattttcaa ataatttcac agaatataat ttagtcaaaa ttaagtttat tactaaacta
    24301 aatcttgtag gtaagtaggt gaataaatca cttttaaatt agaagagctt tatggcaaaa
    24361 taaaaaaact aaaaatcaag tactatatta ttttcagcac ccccgctacc catgaccaat
    24421 ttccaccttg ccgggtgctt aaaccgttca tagaatggca cataaaactt gaattgataa
    24481 tattcatttt gattttaatt gtatttttct tatatagtga agtgctttac atcatcaatt
    24541 aatgataaga tgtttaaata ttcgtatgcc ataaatttaa ttgcatacca gatctgcgga
    24601 gttaaggtta actcttccaa gtttactctt tgattaaggg aaatcatgta cttactgatt
    24661 gccatattgc taatcgccgc catttactcg acaaagataa cctcaaaatt tggggttccg
    24721 gtgcttctgc tatttctggg gatcgggatg ctatgcggca gtgatgtgct caatctgatt
    24781 tattttgaca atgctgtact ggctcaaaaa gtagccaata tctttttaat attcatttta
    24841 ttcgaaggtg gttttcatac ccgtcgcgaa atagtgcaat ctgttttcgg tccggctttg
    24901 acactcgcga cgttcggtat tgctctgacg gcgggaattt taggacttct gatacatttt
    24961 attatggggc tcgatttact ttattcgttg ttgattggct ccatcatttc ttcaaccgat
    25021 gccgcggcag tatttatgat tatgcgtcag cgtgccatta aaaagcgggt ttccttgaca
    25081 ctggaagtcg aatcggctac caatgacccg atggctataa tgttgacaat tgtttttatt
    25141 caaataatta cagggcatcc ccagaacatc ggacttttta tcctcaattt aatctggcaa
    25201 tttggtggtg gaatagtggt aggctggctt attagtaaag tcggcgcgtt tctgttcaat
    25261 cacctcaagg tggaaaatcg tggctattat catgtgctaa gcatagcggt ctgcttgctg
    25321 gcgtatggaa gtgcagaggt tgtcaaagct aatggcatta tcgcggtttt ttttgccgga
    25381 tattggttag gaaatacgga ttttgcttat cgcaaagggg tcggcaattt tattgaaggg
    25441 atttcgactt tctctaatat cgcgatcttt ttactgctgg gagttctggt ttttcccaaa
    25501 agcttattgt cggtttggca agaaggatta ttaattactg cgataatgat cttagttgct
    25561 cgtccactga ctattttgat ttgcactctg ccatttaatt ttaaatttcg agaaaaagtg
    25621 ttcctgatgt ggggcggcat caagggggct gttcccattg ttttggcaac ttatccagct
    25681 gtttatggca ttgaccagaa tcattttatc ttcaatattg ttttctttgc cgtttttttg
    25741 tcgtgtttat tacaaggggt cacacttacc tggggcgctt cgaaattacg actctgtatt
    25801 ccatctcgac cgaaaccaat ccattcgatt gaattgatca cgacccgaaa aaccgatgtg
    25861 gatgtctttg aaattcagtt atccgataat tccaaaattg acggtttaca aatccgcgac
    25921 ttagctttac cgcccgattc cttaattatt gccattattc gagatgatca aatcattccg
    25981 cctaaaggtc atactgtatt gaaaaaaggt gatattttat tcgtaattgc cgctcaggac
    26041 aatgtagaac ggataagcga aattctaagt aaataaccta aaataagtaa aagaggtcga
    26101 ggcagagaag ggtcattagt cattggtcat tagtcattag taaagaggtt gagtaaatag
    26161 taaagaggct tagtaaaaag gagaacccaa aatctgaccg ttcaa
//
LOCUS       DAOOMU010000020        26069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1734_length_26069_cov_24.078881, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000020 DAOOMU010000000
VERSION     DAOOMU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 26069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26069
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_26069_cov_24.078881"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLI55_03225"
     CDS             complement(<1..181)
                     /locus_tag="PLI55_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99881.1"
                     /translation="MKKQILYLIPMVCAVIAVTSFAQSTSPKTATTDKFFEVDQSIEE
                     LRQEIAEIRRQIRDIE"
     gene            complement(178..1272)
                     /locus_tag="PLI55_03230"
     CDS             complement(178..1272)
                     /locus_tag="PLI55_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99882.1"
                     /translation="MKAKNIIIIMLVLGGLMSCAPETATIDPRQVEKLRVDYNRGKIQ
                     KLDDLIAIYQDQAQPIETRIAAVNALAETQHPDAIKAMHDFMDQSVGLNYALLNATAT
                     ALTKHATPDNVDAMVEGIVSAQKKYINFRTGVMEKMEDLNVALQVEALLKLYQSEKEN
                     YVLMQESLTKLLGSIPDDRVIPILINIAKDESVNISTRSLAMEILGKKRHPLITETFI
                     EMLGDPEQQLALRDFAIKSIEDVKESRVILALLETLNQSREEHLVLLDALTRAMGDYS
                     DPAVIPALVEIAKNTDYPVATRRNALSALVKFQDIEAFKQLLPIMESPEGYVLYDEMA
                     QIATAIGQPEVLNQLRLAALKAQLKAREAQ"
     gene            complement(1326..2108)
                     /locus_tag="PLI55_03235"
     CDS             complement(1326..2108)
                     /locus_tag="PLI55_03235"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPA99883.1"
                     /translation="MNQNVYFIADIHLHLNDSQEEKVKRERLFAFLAEVRRHQGSLYI
                     LGDMFDFWFEYRYVIPGVFFPVLCELRETVLAGCEVHFLGGNHDYWAGQFFTDVLGIM
                     VHYKPIDTIIAGKSFHLTHADGILHNDRGYRLMRFFFRNKTIIRLFRYLHPDCAIRLA
                     THLSGRSRRLTLRSPAQEESDRQEIIAYGKKKLEAGAEYVITGHLHLPTEYHTERGVV
                     VNLGDWMKYYSFAFFDGKSLRLCYWPLSTSDSTGKKSEFLLD"
     gene            2350..3435
                     /gene="buk"
                     /locus_tag="PLI55_03240"
     CDS             2350..3435
                     /gene="buk"
                     /locus_tag="PLI55_03240"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPA99884.1"
                     /translation="MIETVLVVNPGSTSTKIALFDRSRRLQFENISHVGTPISRIHSI
                     AEQLPLRIEIVTNTIKPWLFDYCLKAVVGRGGLLKPLRAGVYKITPGMITDAISCKYG
                     EHASNLGAPIAEVIANQHHISSFVVDPVTVDEMIPEARISGIPEIERVSRQHTLNIRA
                     CVRKASAQLGRLPSELNFVVAHLGGGVSIAAVRGGRLIDCNNALLGMGPFTPERAGAL
                     PLEGILNLAYSGKYTKEEMKIKMTKKSGLVAYLGTNDAREVWQRAETGDQRAALIFRA
                     MIYQVCKEIGAMATVLKGNVDAIIVTGGQARGAETVEMIHAGVGFIAPLIVFPGENEM
                     EALAEGGWRALDGNEEILIYEEINVKE"
     gene            3439..4344
                     /locus_tag="PLI55_03245"
     CDS             3439..4344
                     /locus_tag="PLI55_03245"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPA99885.1"
                     /translation="MLKTYSEIFEKIKKNARRPTIAVALAEDKSVMEAIKVLSANDIA
                     RVILTGNPKKIKSYAEQVGYPIKEDEVIPAFSEEEGATRAVELVHLGKAEILMKGHIT
                     TPALMKAVLDKEKGLRSGDLLSHITVVAVSNYHKLLMVTDGGVNIAPDLEKKKAIINN
                     AIIFAKKLEITRPKIAVLAPIEKVNPKIPETVDAMTLQEMAERGEFGSAIVEGPVAAD
                     VALSARAAERKGLHSRIAGDTDIILVPGITCGNAIIKFLMLLAKAEVGGVVVGGRVPI
                     VLLSRSDEPAEKLNSTLLALLMSAL"
     gene            4450..5469
                     /locus_tag="PLI55_03250"
     CDS             4450..5469
                     /locus_tag="PLI55_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPA99886.1"
                     /translation="MSFEKLIKPGTEFLGVKYPIICGAMTWVSDYNLVKTVSETGAFG
                     VLAGGNLPPDLFEQEVDRCIAGVKKPFAVNLITIGPNFRIHYEILQSKNVPFVVFAGG
                     IPRRQDIEGMKTRGKRTLSFAPEESIARQQIEYGIDALILEGSEAGGHIGYVSLIVLL
                     QQVLFKVKGLPIFVGGGIADGRMIAHLLLMGAAGVQMGTRFVLTEECRVHPNFKAALL
                     KAKARQAIATPQYDSKLPVVAVRAIKNKGTDEFGKLQMRLLQEIAAGKITREKAQYEV
                     ENFWVGALRRAVVEGDVDYGSLMAGQSVGLINEIVPMQKMINDLIKEARAEIKVIRNR
                     LGQCG"
     gene            complement(5491..6321)
                     /gene="ybgF"
                     /locus_tag="PLI55_03255"
     CDS             complement(5491..6321)
                     /gene="ybgF"
                     /locus_tag="PLI55_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HPA99887.1"
                     /translation="MNFKPIRPIIYGLFVISLTACATQKDLLKIQEDLYFLRNQIQSV
                     KQTTEENKQLLSQVPTIAVQCNQILIELDSLEKSLAELQDANDRTRADINTRLSSLKQ
                     DAAIISSKLDDASYRSSQLIGKVESLTGKMTNISEKIESRAATPNSAEPAPTEIFNNA
                     YRDMTKGNIELAIQGFQTFLKLYPEKELADYCQYYLAEIAYQQEDYATAATEYKLLIN
                     NYPRSQKITNAHYKLGLCYLKLNNLEQARTQFETVIREYPNTEEALLARNQLQKLEQQ
                     "
     gene            complement(6353..6967)
                     /gene="pal"
                     /locus_tag="PLI55_03260"
     CDS             complement(6353..6967)
                     /gene="pal"
                     /locus_tag="PLI55_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HPA99888.1"
                     /translation="MKKLLVFLIPLLCIVLMFNTCSKKTAGVQEDKGETPAKVTPAPE
                     QPKPVQKPDTLAQAPAPVVTPPPAEPKPVIADTQKVAAIAEPSLKDELAQCNTVYFDY
                     DRYDLRPDAIATLEKAAKVLKQYPNAIITIEGHCDERGTVEYNLALGERRAHAVKTYL
                     INYGIKPDNLITISYGKERPIDPGHNEEAWAKNRRAVLVTSDSK"
     gene            complement(7028..8221)
                     /locus_tag="PLI55_03265"
     CDS             complement(7028..8221)
                     /locus_tag="PLI55_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HPA99889.1"
                     /translation="MFLKIQSDETRKINVAIAEFGTNQNPELAINLRQIIIDDLNYSG
                     FLNVVDPLISPAGDLIFDNSEVIITGDVTHKSSYFNFVFGLTESVTGRKLMTKSLRSY
                     PFALRVLAHTVSDEIVYQLCGEYGNACTKIAYVTEQKGGKELALMDWDGQRATLLTRN
                     RSINLLPCWSNDGESLIFTTYAFGGGPILARYIFKDQQIERFIARAGTFTSPEYSPNG
                     KKVAFVLTHEGNADIYTADRNGQNLQRLTSNPAIDTAPSWSPNSREIVFTSDRSGSPQ
                     IYIMDAEGGNVRRLTYENNYNSSPKWSPKGDQIVFVSNQSHGFQIYLIDVTGENLICL
                     TDNTGSYENPTWSPNGSHLVCASNRSGRWEIYSMLRDGSLLRQLTFTGGNTGPAWSPR
                     LKPFN"
     gene            complement(8298..8978)
                     /locus_tag="PLI55_03270"
     CDS             complement(8298..8978)
                     /locus_tag="PLI55_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPA99890.1"
                     /translation="MNYTFAISLILHLTFFAVVSVLAGRHQPRVMAIPDQNIRWVNIA
                     PPVVQTAEAPIQVKPATSVPVPKPKVEKAATVLAEKPQKPQETKPLPEETIASSTTSP
                     ELPVEPEATSQPASGGIPGATGLRVDDPDFNFIYYLNIIRNRIQEHWRPPYSSADSPY
                     SQQVMIAFKITRSGKISDVRIEQSSGNFLFDQAALRALYETATLPPLPSEYGGKELNV
                     HIEFETLK"
     gene            complement(8975..9379)
                     /locus_tag="PLI55_03275"
     CDS             complement(8975..9379)
                     /locus_tag="PLI55_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPA99891.1"
                     /translation="MVDRRYKPLTEINITNLVDVTLTVLIIFMITTPLMYSGIEVDLP
                     ATRREITTVQEGILVNYSLDGQIFIDGQPVIENEFEKTLVKRWVQVGKKPVLLSADRK
                     IEYGKIIKLIDRIKACGVTELGLIVETEQDKK"
     gene            complement(9360..10067)
                     /locus_tag="PLI55_03280"
     CDS             complement(9360..10067)
                     /locus_tag="PLI55_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPA99892.1"
                     /translation="MNTIFSSTYDIGIMGWVIILILIGLSIIAWGIIIEKMILFRRTE
                     KQNKKFLAIYQSYSDWSAVARETQHLKSGLLLNLFRKIYAEARKDAELNPGNVVTENI
                     DTDASRISSLELYKALVDSAISTELIPYEKRLIFLSSTVSASPFLGLFGTVWGVMQAF
                     LSIGVKGSAAITAIGPGIAEALITTVVGLAVAIPVLFAYNLIVDKIRKYENQLQILAN
                     DMIQKIIRENFYGGSQI"
     gene            complement(10209..12266)
                     /locus_tag="PLI55_03285"
     CDS             complement(10209..12266)
                     /locus_tag="PLI55_03285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPA99893.1"
                     /translation="MKSKAVGLIWLSLIIMVDIGFTAKRSMQIDDLLNCHRLAEPQYS
                     PDGQWIAFTVQTIDIEANKGQKTLWRIGPDGQNLQQIPTHSGNSWSPLWSPDSKSLAF
                     LSDSGGQVQVWKLALDNNHVQQITDFYGGVESFCWSHNGQKIAFSARVYPDLTDFDEM
                     KKRDATRQASPIKARVYEELMFRHWDEWWDYKRSHIFVLDLNNGSLTDVTPGDFDAPP
                     IALGEGYCFSPDDQTIVFNSNHDPVIAISTNNDIWSASIANPREPKLITTAQEYRDFR
                     GNDYAPRFSPDGKYLSFLSMARAGFEADKSDLFLYEMATGKFINLTEKNDIRIQAYEW
                     LPDSRGLLLLIEERGRLTLKTLNIKSGQIKTLVKEGYNSAVTLAPDGRSCAFLQSRTT
                     SPDELFVYSFTDNKIRQITDFNSPLRSQIEMNDVEDFEFKSSDGAMVHGFLITPPFFD
                     AREKYPAVFLVHGGPQSAWEDNWHYRWNPQLWSAQGYVIIMINPRGSIGYGQKFTDAI
                     SLDWGGQVFDDLVVGQKYVIDHFKFIDADKLAAAGASYGGYMMNWMEGHMEAFSYPFK
                     TLVNHDGSFNLYAMYLTTEELWFPEWEYGGVFWENRDQYEKFSCDNYIINFKTPMLII
                     HGEKDYRLDPSQGIMAFTALRRLNIPAKLVLFPDEGHFVLQPQNSRFWHQTIFSWLEK
                     YLR"
     gene            complement(12291..13220)
                     /locus_tag="PLI55_03290"
     CDS             complement(12291..13220)
                     /locus_tag="PLI55_03290"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPA99894.1"
                     /translation="MDLTPESPTPLINNYKKGVFPADCLVFGFTVLMLLIGITNYSRL
                     GKPYTWISTDCGIIIVLLMLIFKETHEERRFFYWLHFFWPIISLAFLYTQCTSWDNLI
                     FPITFDPVLIKWDLRLFGSALNKVMASSVDSLWLDEIMHFFYFSYYLVIFLTVVWMLG
                     RRQPQAYELIFSLTLMMYFHFIFFMIFPSDGPLADRNDLFNQGVVFIPLMDFIYGVSE
                     QNGGGAFPSTHVSAAVVVAIYTFKNSPKLRIPIGGLCLGIIISTVYCSYHYAIDAAAG
                     IITGTLFYFLGKYIYNHRFQPVNLSLFMPQKSI"
     gene            complement(13211..14197)
                     /locus_tag="PLI55_03295"
     CDS             complement(13211..14197)
                     /locus_tag="PLI55_03295"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPA99895.1"
                     /translation="MKALVTGATGFIGQHLIRRLLAENCEVRALCRSTSIKPDEFAQS
                     VEWQIGDITAPESLASAVSDCDLVFHLAGAIKAQDDATFTRINVNGTVNLLKALKQYG
                     KKEVRFIFVSSLSASGPSEPWSPKNESEPCYPVSAYGQSKLEAEIEVLKQKNRIWCTI
                     IRPAVVYGPGDKESLAFFKIAKSRFNPHLGFEKRYVGMIYVGDLVDLLWKAALSDRPS
                     GEIYFACDEQSDGYEWNQIIATAGKVLGYHTLPILVPQFILPMIVVPTQIIAKMTGKI
                     GSLNRDKYREMNQSHWTCTSAKAQKLLDFKPQVDLSEGFKRTAQWYREQNWI"
     gene            complement(14197..15036)
                     /locus_tag="PLI55_03300"
     CDS             complement(14197..15036)
                     /locus_tag="PLI55_03300"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPA99896.1"
                     /translation="MLKPLAIESWPDMFFYRPLAFGLVKTIIPLPITPNMISLSAIII
                     GLSGGYCLAIGTPRICQIAGLLFMANVILDCADGMLARYKHNGTPLGFIVDGLVDYCN
                     GAAIFIGMTVGFSRTDSQFGFSLWVLIPLSLFSWIAHSMAVDHARREFQRNALEQCPS
                     LAGGRQNFQNYREQLSRSKGHWSDKLLASLYLFYCNVQKPNAKFSRTYPADRYYRYNN
                     CVMRGWLLIDPSTHMTIAILSALLYNPLIFFWYTIVIANIIMLMMYLIQYFVNQKVGV
                     LPV"
     gene            complement(15121..15843)
                     /locus_tag="PLI55_03305"
     CDS             complement(15121..15843)
                     /locus_tag="PLI55_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99897.1"
                     /translation="MDNIFDIIILNGRPASGKSEVIDYLKKTPVEIRRRRFHIGELDE
                     IDDFPMLWTWFEEDAILEKILNKPRLHSDKDGYFFWEYLWHLLIERISMEYSKRLRDK
                     PNFHKNYTALIEFSRGSEHGGYRAAYPHLSDEILKRAAIFYIDVSFEESLRKNRQRFN
                     PAKPDSILEHGLPDAKLTRLYKDCDWEEFRGNDPEFIKIKGLKVPYVVLHNEPDITTA
                     RDENLGRVLEELLNKLWILRQK"
     gene            complement(15862..17241)
                     /locus_tag="PLI55_03310"
     CDS             complement(15862..17241)
                     /locus_tag="PLI55_03310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPA99898.1"
                     /translation="MNTSETENLLAMPTFRHRRLQNWIIIGLLYSFFYMSRYNFSAIA
                     PTLQAFFGWTKSNLGVFETLMPLVYGLAVVINGPLADKIGGRKAFLIGALGVVIMNGL
                     FGFFHILVQTPAVWADQEIVSEAVLKFGFSKSALLWTLAIIWAINGYFQSFGALSIVK
                     INAQWFHIRERGTFSAFFGVLIRFGLILAFSGTPLIVKFLPWYWAFWIPALVVALLFV
                     LTIFFVRDTPAEAGFPELDTGDGDVNLEEKVKFKEVLTKVFGSKVMWTIAIGSMMIGF
                     VRRGVVDAWWPIYFKEMQGVTGVDFAFQLTAWGIAILGIIGGFAFGISSDRVFGGRRA
                     PVIVIGFCGMIVILTLFFLADVWQWGPIAAAILLACLSFFVNGSHGMIAGAATMDFGG
                     RKAAATAVGLIDGMQYIAGAFVGALVGWVTTNWGWQAWKIWPIPFAILGALVMSSIWH
                     TTPTGKSTH"
     gene            17365..19020
                     /locus_tag="PLI55_03315"
     CDS             17365..19020
                     /locus_tag="PLI55_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPA99899.1"
                     /translation="MEMIEVNLGETRTITVQPGQKIIEIVKQALPHNRYKPYIVIRNN
                     TFTSFNEIVNEPTKISLLRHFHESSRRAYESAAILILNYVVEDLYPDQRLIVMHSMCD
                     GVFCRFVNEDFCTPQIAQEISAAFIKVVEADYAIEPLLISCNDARRYFKEIGQEDTEE
                     LLKYSEANFVTLYQINGRRFWLLSPPAPCTGLIKVFEIRPYQKGFVLRCPTEGDDRRL
                     RPFTDQARLYEIFQETEEWGRILEISTVADLNRTIVENQISDLIKIDEALHEKKIASI
                     ADQISNKGERRFVFVAGPSSSGKTTFAKRLAIQLRVLGFKPLLLSLDNYFRDRAELAK
                     LQGKRINYEVLDAIDLELLNDHLQRLLRGETITPPVYNFITGTKTPATKPMKIDSQTI
                     LIFEGIHGINPGLTPEVNERYKFKIYVSALTHLNFDHMNRVRTHDTRLLRRIVRDYKY
                     RGYSAAETIEMWKKVVEGENKYIFTFQGNADIMFNSSLVYEHGVLKTLAVTMLKNVPE
                     NHHSYPEAQRLLDMLSYFLPVDDTEIPPTSIMREFIGKSSFTY"
     gene            complement(19096..19515)
                     /locus_tag="PLI55_03320"
     CDS             complement(19096..19515)
                     /locus_tag="PLI55_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPA99900.1"
                     /translation="MALSKGSVNKVILVGRLGRDPEVRYTPNGDAVAEVSLATTESVK
                     NRDGKREDRTEWHNLVIWRNQAEFAKEWLKKGQLVYVEGRLQTRQWEDKEGQKRSKTD
                     IQVDQITMLGGSGRGREKEAAEPETPPEPSPDDEIPF"
     gene            complement(19617..20804)
                     /gene="kbl"
                     /locus_tag="PLI55_03325"
     CDS             complement(19617..20804)
                     /gene="kbl"
                     /locus_tag="PLI55_03325"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPA99901.1"
                     /translation="MYGKLQQDLQSQLESITKQGLYKSERVIASPQAPHLRLTDGREL
                     LNFCANNYLGLSNHPVIIQSAQLALERWGFGLSSVRFICGTQEIHKELERRLSDFLGT
                     EDTILFSSCFDANGGVFEPLFDEQCAIISDELNHASIIDGIRLSKAQRFRYSHMNIDE
                     LQKCLENSAQAKYRIIVSDGVFSMDGHIAPLPQICELAEKYDALVMIDDSHATGYLGR
                     TGRGTAEYYGLLGKIDIITTTFGKALGGASGGCISGRREIVEFMRQRSRPYLFSNSVA
                     PAVIGATLKALEIVQENPDLPRRTIENAHHFRQKIEAAGFDIIPGNTAIIPIMIYDEP
                     LAIKIADLLLEEGIYVIGFTYPVVPRGTARIRVQVSAAHTPEQIDRAVAAFTKVRKSL
                     NKG"
     gene            complement(21067..21759)
                     /locus_tag="PLI55_03330"
     CDS             complement(21067..21759)
                     /locus_tag="PLI55_03330"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HPA99902.1"
                     /translation="MNPSIQEMFTTVPPTYRILNQILTLGQDNVWRKRAARLATSAGG
                     RRWLDACGGTGEMAIELSRLANYGTSIFVADFSLPMMGKAREKSGAKHIIFTLANMKC
                     LPFQSDTFDVITISFATRNLSISQDNLHQCLGEFQRVLKPGGLFVNLETSQPRWTPIR
                     RLFHLYVKATVKPIGRLISGSENAYSYLSHSIRHFYSPEELAEIIRQAGLREIGFKRM
                     FLGAAAIHKAIK"
     gene            complement(21831..22106)
                     /locus_tag="PLI55_03335"
     CDS             complement(21831..22106)
                     /locus_tag="PLI55_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPA99903.1"
                     /translation="MMTPEKFKENVLTLANDVGVKPREIHIQPMKRKWASCSSKGRLT
                     FNKKLLKEPEEVRLKVILHELLHLKYPNHGKMFKLLMDTYLQKSLLK"
     gene            complement(22103..25054)
                     /locus_tag="PLI55_03340"
     CDS             complement(22103..25054)
                     /locus_tag="PLI55_03340"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HPA99904.1"
                     /translation="MSKLGGEKYSVQQPIIDYVREQSAEYVAQNGARVFLKLGWQYVT
                     PDEALRLRGGETGMVFKEVFSDQLQKLNPDFKDSSMAEEIVKKIESVPSNIEGNFIAW
                     EYLKGLKTVFVPGEKRERNIKLLDTENLDNNVFHVTDEFKFTNGIKTIRLDIVFLING
                     IPVLFIETKSSDKIEGMSEALEQVKRYHRECPELLAVLQVYALTHIVQYYYSGTWNTS
                     ERFLFNWKEESGGDFEHLVKTFFDRERVVKLLVDFMMFTRQDDALLKVILRPHQMRAV
                     DKIVERAKDPEKHRGLVWHTQGSGKTYTMIIAAQKIIANPLFENPTVIMLVDRNELES
                     QLFGNIAALGIGNVEVTESKEHLRNLLSSDRRGLIVTMIHKFEGIPANINTRKNIFVL
                     VDEAHRTTGGKLGNYLMGALPNAAYIGFTGTPIDKTQYGNGTFVTFGKDDPPKGYLDK
                     YSIAESIEDGTTVPLYYTLAPNELRVEKEALEKEFLNLAEAEGISDIEALNKVLEKAV
                     TLKNMLKNKERVEKVARYICDHYKNYIEPMGYKAFVVAVDREACALYKEELDRYLPPE
                     YSEVVYSSAFNDSPELAKYQLSEIEEKRIRKAFRKSNELPKILIVTEKLLTGFDAPIL
                     YCMYLDKPMRDHVLLQAISRVNRPYEDDEGRKKSSGFVLDFVGIFDNLEKALAFDSKD
                     IEGIVRDIQVLKNRFENEMKLGREKYLTLIEGKTQDKAVEAALEHFRDEEERQEFYKY
                     FKKLSDIYEIISPDEFLRSYLDDYETLLRMYKIVRENYERGTLIDKMFTRKTALLVQK
                     HTTSSDIRPTLEVCEINETTLRRIEESKASETEKVFNLIKSIERTVEEQSGNSPYLFS
                     IAEKAELLVKMYKMRQKNTQETLEELKRIIKEINDARKEQTEKNMSAENFTIYWMLKS
                     EGFKDAEAMANEMRNLFEKYPHWQKSEKYEREIRTGIYQTITRSGVKDVKEIPEIVKK
                     IMKTLKANREE"
     gene            complement(25051..25629)
                     /locus_tag="PLI55_03345"
     CDS             complement(25051..25629)
                     /locus_tag="PLI55_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99905.1"
                     /translation="MKKGNKKTESDFRNELYEKIKSEFSGYTVCNCGKETNLLYQIII
                     DGNGDVKPKPDELKNPLRGSYAFQTDMLIKKKNIPIVAIEIKFLGITTHDILTYSTKA
                     MKHKEIYPYLRYGLIAGNIEKIPNRFFIHNQGFDFAIALFYNENGTLKDNELESTIKL
                     LKRQIKDAESLLSLLNTESGAKLFNRNVEVTS"
     gene            complement(25633..>26069)
                     /locus_tag="PLI55_03350"
     CDS             complement(25633..>26069)
                     /locus_tag="PLI55_03350"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPA99906.1"
                     /translation="KSSAKYVENNTLLIAMYGATVGKVGLAKAKFTINQAICAVNLNE
                     KLWPEFYFFYFQMIRNKLLSMRFGGAQPNINQQIIKQISLPLPPLPIQQKIASILSAI
                     DAKIEAEENKKKALEELFKSLLHNLMTGKIRVNNLKLNEKDI"
BASE COUNT         7174 a         5678 c         5851 g         7366 t
ORIGIN      
        1 gttcgatgtc gcggatttgt cggcgaattt cggcgatttc ctggcgtagt tcctcaatac
       61 tttggtccac ctcgaagaat ttatcagtag tagcggtttt cggactggtt gactgagcga
      121 aggaagtgac cgcaattacg gcgcaaacca tcgggattaa atatagaatc tgttttttca
      181 ttgagcctcc cgagctttta gctgggcttt caaagcggcg agtctgagtt gattgagaac
      241 ttccggttga ccaattgcgg tggctatctg agccatttcg tcatagagca catacccttc
      301 tgggctttcc ataatcggca ggagttgctt aaaagcctcg atgtcttgga acttgaccag
      361 agctgatagt gcattgcggc gagtagccac cgggtaatcg gtgtttttcg ctatttcgac
      421 caaagctgga ataacggctg ggtcagaata atcgcccata gctcgggtaa gagcgtccaa
      481 cagcaccaag tgttcctccc gactctggtt tagagtttct aacaacgcta aaataacccg
      541 cgactctttg acgtcttcaa tggatttaat ggcaaaatcg cgcaatgcca attgctgttc
      601 cgggtcgcca agcatttcaa tgaaggtctc tgtgattagt ggatgacgtt tcttgccgag
      661 aatttccatc gccagtgagc gcgtcgaaat gttgacgctt tcatctttgg cgatattgat
      721 caggatggga atgacgcggt cgtcgggaat cgaacccagc aatttggtta acgattcttg
      781 cattaagaca tagttttcct tttcagattg atacagttta agtaatgctt ccacctgtag
      841 agcgacgttc agatcttcca ttttctccat tacgccagtt cggaaattga tgtatttttt
      901 ttgggcagaa acaatccctt ctaccattgc atccacatta tctggtgtgg cgtgcttcgt
      961 caaagcggtt gccgtggcat tcagtaaagc ataattgagg ccgaccgact gatccataaa
     1021 atcgtgcata gctttaatgg catccggatg ttgagtttca gccagggcat tcacggcggc
     1081 gatgcgagtc tcaataggtt gcgcttggtc ttgatatatt gcgattaagt catcaagctt
     1141 ctggattttt ccccgattgt aatcaacccg cagtttttcg acttggcggg gatcaatggt
     1201 ggcggtctca ggagcacagg acattaagcc gccgagaacc aacatgataa ttatgatatt
     1261 ttttgctttc atgtgtttct tccgttcttt atttatccca aatgatttta gaacatcttt
     1321 atttcttagt caagtaaaaa ttctgatttt ttaccggtgg aatcagaggt gctgagaggc
     1381 caataacata ggcgcagtga tttgccatca aagaatgcga aggaataata tttcatccag
     1441 tcacccaggt tcaccacaac tcctctctcg gtatgatact cagtcggcaa atgtaaatgg
     1501 ccggtgatga catattcagc gcctgcctcc aattttttct tgccataggc aatgatttct
     1561 tggcggtcgg attcttcctg agccggactg cgcagagtca gacggcggct tctcccagat
     1621 aaatgggtag ccaagcggat ggcgcaatct gggtgaagat agcgaaatag gcgaatgatg
     1681 gtcttattcc tgaaaaaaaa ccgcatcaac cggtatccgc ggtcattatg aaggataccg
     1741 tcagcatgag tcagatggaa gcttttcccg gcaattatcg tgtcaatcgg tttatagtga
     1801 accataatgc caagcacgtc tgtgaaaaat tgccccgccc aataatcatg attgccacca
     1861 agaaagtgca cctcgcatcc ggctaaaacc gtttcgcgta actcgcataa caccgggaag
     1921 aatacgcccg gaatcacgta acgatattca aaccaaaaat cgaacatatc accaagaata
     1981 tataatgaac cttggtgacg tcggacttct gccagaaacg caaaaaggcg ttcgcgcttt
     2041 accttttctt cctgactatc gtttaagtgc aggtgaatgt cggcgataaa atagacgttt
     2101 tgattcattg tgtttaaaat ttaaaagaat agtaagatat acaaaagact atttctctac
     2161 tcctgctcgc cgattaattt cattttatgg gaaaataaat tagataattc ctaaaattta
     2221 gaaataacag cggtcccttc tgtctctttt aaaatgtact tcacgatgat aaagtaatca
     2281 atggcatcat atttgtctgg caatgggtta gagatgtctg acaaatacaa ataagaagca
     2341 aagcccagta tgatagaaac agtcttggtg gtaaatcctg gttcgacgtc aaccaaaatc
     2401 gcgctattcg atcggagtag gcgactccaa ttcgaaaata tcagtcatgt cggtactccc
     2461 ataagtcgga ttcactcgat cgcggagcaa ttacccttac gcatcgagat tgttactaat
     2521 acaattaagc cctggttatt tgattattgc ctgaaagcag tggtcggtcg gggcggtctt
     2581 ctcaaacctc tgcgggcagg tgtgtataaa attaccccgg gaatgattac cgatgcaatc
     2641 agctgtaaat atggggagca tgccagtaat ttaggggctc cgattgccga agtgatcgct
     2701 aatcaacatc atatttccag ctttgtggtg gatccagtta cggttgacga aatgattccg
     2761 gaggcgcgca tttcaggtat tcctgaaatt gaaagagtga gccgacaaca cactttgaac
     2821 attcgggctt gtgtgcgcaa agccagcgcg caactgggtc gcctgcctag tgaattgaat
     2881 tttgtggtcg cccatctggg aggcggtgtc agcatagcgg cggttagagg tggtcgtctg
     2941 attgattgca acaatgcttt gcttgggatg gggcctttca cgccggaaag agccggagct
     3001 ttgccactgg aaggtattct gaatttggcg tattcaggga aatacactaa agaagaaatg
     3061 aaaataaaaa tgaccaaaaa aagtggtctg gtcgcttatc tcggtacaaa cgatgcccga
     3121 gaagtctggc aacgagcaga gacgggagat cagcgggcgg cattgatatt cagggcgatg
     3181 atttaccagg tttgcaaaga gattggcgcg atggcgactg ttttgaaggg aaatgtggac
     3241 gcaataatcg ttaccggcgg acaggcacgt ggtgctgaaa ccgtcgagat gatccacgct
     3301 ggggtgggat ttattgctcc gttgattgtt ttcccgggtg aaaatgaaat ggaagcactg
     3361 gctgaaggcg gctggcgagc cctcgatggg aatgaggaaa ttctgattta tgaagagatt
     3421 aatgtcaaag aataacttat gctaaagacc tattccgaaa tatttgaaaa aattaaaaaa
     3481 aatgctcgta gaccgacgat agcagttgca ttagctgaag ataaaagtgt catggaagct
     3541 attaaagtcc tatcggcaaa cgatatcgcc cgggttattt taaccggcaa tccgaagaaa
     3601 attaagtcgt atgctgaaca ggttggttat cccataaaag aagatgaagt aatcccggca
     3661 tttagcgagg aagaaggagc cacacgggcg gtcgaactgg tgcatttagg aaaagccgag
     3721 atcctgatga agggacatat tacgacgcca gctttaatga aagccgtatt ggataaagaa
     3781 aaaggcttgc gcagtgggga tcttttaagt catatcacgg tcgttgccgt ttccaactat
     3841 cataaattac tcatggtcac cgacggcgga gttaacatag cgccggatct cgagaagaaa
     3901 aaagctataa ttaacaatgc cattattttc gccaaaaagc tagaaatcac cagaccaaaa
     3961 atcgcagttt tagccccaat cgagaaggta aatcccaaaa ttccggaaac agtagatgca
     4021 atgactttac aagaaatggc tgagcggggt gaatttggtt cggcaattgt agaaggccca
     4081 gtagcggctg atgtcgcgct ctcagcgaga gccgccgaac gtaaaggttt acatagtcgc
     4141 attgccggtg atacagatat tatactggta ccgggtatta cctgcggcaa tgctatcatc
     4201 aagtttttaa tgttgctggc taaggctgaa gtcggcggag tggtagtagg tgggcgggtg
     4261 ccgatagttt tactttcgcg ttccgacgag ccagcggaaa aattgaactc gacactttta
     4321 gctcttctaa tgagcgccct ataatttatc agtcaattaa cgaaatttcc cgcggcgtag
     4381 aaactgaaat tgctcagatt ttgtaaattt tcctgcgttt gaaatttaat tttaacaaaa
     4441 ggtgaaataa tgagttttga aaaattaatc aaaccgggca ctgaattttt gggcgtcaaa
     4501 tatcctatca tttgcggcgc tatgacttgg gtgagcgatt ataatctggt aaaaacggtc
     4561 agtgaaaccg gggctttcgg agtactggca ggcggcaact tgcctcctga tttattcgag
     4621 caagaagttg accgctgtat tgctggtgta aagaagccgt ttgcggtcaa cctaatcacg
     4681 atcgggccta attttcgaat acattatgaa attttacaaa gcaagaacgt cccatttgta
     4741 gtttttgccg gtggtattcc tcgccgtcag gatattgagg ggatgaaaac ccgcggcaaa
     4801 cgaacgctat cgttcgctcc cgaagaaagc atagcgcgcc aacagattga atatggcata
     4861 gatgctctga ttttggaagg cagtgaagcc ggtggacata tcggttatgt ttcattgatt
     4921 gttttacttc agcaagtctt gtttaaagta aaaggtttgc cgattttcgt cggtggtggg
     4981 atcgctgatg ggaggatgat cgctcacttg cttctaatgg gtgcggctgg ggtccaaatg
     5041 ggaacccgct ttgtgttaac tgaagaatgt cgggttcatc cgaattttaa ggcggcatta
     5101 ctgaaagcca aagcgcgcca ggctattgct actccacaat atgattccaa attgccggtc
     5161 gttgccgtac gagctatcaa gaacaaagga acggatgagt tcgggaagct ccagatgcgt
     5221 ttactacagg aaatcgctgc cggtaagatt acgcgggaaa aagctcagta tgaagtggaa
     5281 aatttttggg ttggcgcttt gcgtcgggcg gtggtggagg gtgatgtaga ttatggctcg
     5341 ttaatggccg gtcagagtgt tgggctaatt aacgagattg tcccgatgca aaagatgatt
     5401 aatgatctga taaaagaagc gcgcgcagaa ataaaagtta tccgcaaccg tctgggacag
     5461 tgcggataac ttttggattt tccttccgga ttattgttgt tccagcttct gcagctgatt
     5521 acgggcaagt aaggcttctt cagtattggg gtattcccga ataactgttt cgaactgggt
     5581 gcgtgcctgc tccaagttgt ttagttttaa atagcataat cccagtttgt aatgggcgtt
     5641 agtgattttc tgactgcggg gatagttatt aatcaatagc ttgtattcgg tcgcagctgt
     5701 ggcgtagtct tcctgttgat aggcgatttc ggcaagataa tattggcaat aatcggctaa
     5761 ttccttttcc ggataaagtt tgaggaaagt ctggaagccc tgaatagcca gttcgatgtt
     5821 gcctttagtc atatctcgat aagcgttgtt gaaaatttcg gtaggagccg gctcagccga
     5881 gttgggagtg gcggcgcggc tctctatttt ttccgaaata ttggtcattt tgcccgtcaa
     5941 agattcgacc ttgccgatca gctgactgga gcggtagctt gcgtcgtcca atttactgct
     6001 gataatcgcc gcatcttgct taaggctgga tagtcgggta ttgatatcag ctcgggttcg
     6061 gtcattggca tcttgtaatt ccgcaaggga tttttccaga gaatctagct ctatcaatat
     6121 ttgattgcat tgaaccgcaa tagtaggaac ctgacttaac aattgtttat tttcttcagt
     6181 agtctgttta acggattgaa tctggttgcg taaaaaatat aaatcctctt gaattttcaa
     6241 aagatctttt tgagtggcgc aagccgtcaa gctgatcacg aacaaaccat aaatgattgg
     6301 acgaattggt ttaaagttca tagctacctt ggttgatgaa agcgccgaga atttattttg
     6361 agtccgaagt gaccaatacc gctcggcggt ttttagccca agcctcttca ttatggcccg
     6421 gatcgatcgg tctttctttg ccatagctta tcgtaattag attgtccggt tttattccat
     6481 aattgatgag ataagttttt acggcgtgag ctcgacgctc gccgagtgcc aggttatact
     6541 ctacggtgcc tctttcgtcg cagtgacctt cgatagtgat aatggcgttc ggatactgct
     6601 taagcacttt ggcggctttt tcgagagtgg cgatggcatc cggtcgcaag tcataacggt
     6661 cgtaatcgaa atagaccgta ttgcattgcg cgagttcatc ttttagggaa ggttcagcaa
     6721 tggcggccac cttttgtgtg tccgctatga caggtttggg ttcggcaggc ggtggggtga
     6781 ctacaggggc tggtgcttgg gcgagagtat ccggcttttg gacaggttta ggctgttccg
     6841 gggccggggt gactttggct ggagtctcac ccttgtcttc ttgcaccccc gcggtctttt
     6901 tactgcaagt attgaacatt aatacaatac agagtaaagg aattaaaaaa acgagaagtt
     6961 ttttcatttt ggttgctcct ttctttttgt taattagtgt tatactcata caccctgaaa
     7021 tgattttcta gttaaatggt ttcaagcgcg gcgaccaggc tggaccggta ttgccaccgg
     7081 tgaaagttaa ttggcgtaat agtgatccgt cgcgcaacat gctatagatt tcccagcgtc
     7141 cactccggtt ggaggcgcaa accagatggc taccattagg cgaccaggta ggattttcgt
     7201 aactaccggt attatcagtc aggcagatta gattttctcc ggtaacatca atgagataga
     7261 tttgaaaacc gtgggattga ttagagacga aaacaatttg atcgcctttc ggtgaccatt
     7321 taggtgagga attatagttg ttctcataag tcagccgacg gacattgcca ccttcggcat
     7381 ccataatata aatttgggga gaaccgctac ggtccgaagt gaatacaatt tcgcgactgt
     7441 taggtgacca cgacggggct gtatcaatag ccggattgct ggtcaggcgc tggagatttt
     7501 gaccgttacg gtcagcagtg taaatgtcgg cattgccctc gtgagtaagg acaaaggcaa
     7561 cttttttacc gttgggagaa tactccggcg aagtaaatgt gccggcgcgc gcgatgaatc
     7621 gttcaatttg ctggtctttg aatatataac gcgccagaat gggaccgcca ccaaaagcgt
     7681 atgtagtaaa aattaatgat tctccatcgt tcgaccagca tggcagtaaa ttaattgaac
     7741 gatttcgggt caggagagtc gcgcgctggc catcccaatc cataagggct aactccttgc
     7801 cccctttttg ttcggtaaca taagcaattt tagtgcaggc attgccatat tcaccgcata
     7861 attggtacac gatttcgtcc gaaacggtat gggcaagaac tcgcagggca aaggggtaag
     7921 agcgcaaaga cttggtcatt agtttccgac cggtgacaga ttcggtaagc ccgaatacaa
     7981 aattgaaata actactcttg tgagtaacat caccggtgat aattacctct gaattatcaa
     8041 agattaaatc tccggcaggg ctgatcagcg gatcaacaac attgagaaat cccgaataat
     8101 tcaaatcatc gataataatc tggcgtaaat taatagccaa ctctggattt tgattagtac
     8161 cgaattcagc gatagcgaca ttaattttgc gcgtctcgtc cgattgaatt ttaaggaaca
     8221 catctgtttg actacttcca gtgacgttca agaaggatat taggcaaatc aatgatagat
     8281 tttttttcat accacgttta tttcaaagtt tcaaattcga tatgaacatt taactctttt
     8341 ccgccatatt cactcggtaa gggtggtaaa gtagccgtct cgtaaagcgc acgcagagcc
     8401 gcctgatcaa agagaaaatt accggaagat tgttcgattc gtacatcgga aatttttccg
     8461 gaacgggtga ttttaaaggc aatcataact tgctgactgt agggagaatc tgctgaagaa
     8521 tagggaggcc gccaatgttc ctgaatccgg ttgcggataa tgttaagata atagataaaa
     8581 ttgaagtcgg gatcatctac ccttaaaccg gttgcaccgg gaataccgcc agatgcgggc
     8641 tgagatgtag cctccggctc gacgggtagc tccggtgagg ttgtggacga ggcgatcgtt
     8701 tcttcaggta acggtttggt ctcttggggc ttttgtggtt tttccgcaag aacagtagca
     8761 gctttttcta ctttgggttt aggaactgga acggaagtgg ctggttttac ctgaattgga
     8821 gcttcagcag tttgaacgac cggtggagcg atattgaccc agcgtatgtt ctgatccggg
     8881 atggccataa cgcgaggctg gtggcgacca gccaagacac ttacgacggc aaaaaacgtc
     8941 aagtgtaaaa ttaaggatat ggcaaaagtg taattcactt cttatcctgt tcggtttcga
     9001 ctattaatcc caattcggtc acgccgcaag cttttatccg gtcaatcagc ttgataattt
     9061 taccatattc aattttccga tcggcactta ataaaaccgg cttcttaccg acttgtaccc
     9121 agcgtttgac taaagttttt tcaaattcat tttcaatgac cggttgccca tcaataaaaa
     9181 tttgcccatc aagactataa ttaaccagga tgccttcttg gacagtagta atttcgcgtc
     9241 tggtagcagg caaatcaacc tctataccac tatacattaa gggtgtcgta atcataaaaa
     9301 tgattaaaac tgttaaagtg acatcaacta aattagtaat gttgatttcg gtcagcggct
     9361 tatatctgcg atccaccata aaaattttcc cggatgatct tttgaatcat atcattggcg
     9421 agaatttgta attgattctc gtattttcgg attttatcca cgattaagtt ataggcaaaa
     9481 agtaccggaa tagcgactgc caaacccaca acagtcgtaa ttaatgcctc agcaatcccg
     9541 ggacctatgg ctgtaatcgc cgctgagcct tttactccaa tgctcaagaa agcttgcatg
     9601 actccccata cggtaccgaa taatcccaga aacggactgg cactaaccgt agaagataaa
     9661 aatatcaatc ttttttcata aggtataagt tcggtgctaa ttgcggaatc caccagggct
     9721 ttataaagct cgagcgacga tatgcgactg gcatcggtat ctatgttctc agtaaccacg
     9781 ttgcctggat tgagctcggc atctttgcgg gcttcagcgt atatttttcg aaaaagattg
     9841 agaagtaaac cggacttgag atgctgagtt tcccttgcga ccgcgctcca gtcggaatac
     9901 gactgataaa tagccagaaa ttttttattt tgtttttcgg tgcgtctgaa gagaatcatt
     9961 ttctcaataa taatacccca ggcgatgata gaaagcccaa tcaagatcag aataattacc
    10021 catcccatta tgccgatatc ataagtagaa gagaaaatgg tattcatgtt tttaatcctg
    10081 tttttactgc taaaatgact ggtttaattg aatgttccat attaataata atgaatctgc
    10141 gttataagac aaattacgca ctaataagtt aaaatactat gatcggcgta tcccgaagaa
    10201 cttgtgattt agcgtaaata tttctcgagc cagctaaaaa ttgtctgatg ccagaaacgg
    10261 ctgttttgtg gctgtaacac gaaatgtcct tcatcgggaa aaagtaccag cttggcaggt
    10321 atattcaagc gccgcaaggc agtgaaagcc attattccct gacttggatc taagcgataa
    10381 tccttttcac cgtgaataat caacatagga gttttgaaat ttattatgta attgtcgcag
    10441 gagaattttt cgtattgatc tcgattttcc cagaaaactc caccatattc ccattcgggg
    10501 aaccagagtt cctcagttgt aagatacatc gcatataaat tgaatgaacc gtcgtgatta
    10561 acgagggttt tgaaaggata cgaaaacgcc tccatatgac cctccatcca gttcatcata
    10621 taaccgccgt aagaagctcc cgccgcggct aatttatcgg catctatgaa cttgaagtgg
    10681 tctattacat atttttgtcc aacgacgagg tcatcaaaaa cctggccacc ccaatccaga
    10741 ctaattgcgt cggtaaattt ttgaccatag ccaatactac cgcgcggatt aatcattata
    10801 attacataac cttgagccga ccatagttgc ggattccaac ggtaatgcca attatcctcc
    10861 caggcgctct gtggcccacc gtggaccaga aaaacagccg gatatttctc ccgggcatca
    10921 aagaaaggcg gcgtgatcaa aaagccatga accatcgctc cgtcactgct tttgaactca
    10981 aaatcctcaa cgtcattcat ttcgatttga gaccttagcg gagagttgaa gtcggtaatt
    11041 tgccggattt tgttatcggt gaaagaatat acgaaaagtt catcggggct ggtagtccgc
    11101 gactgtaaaa aggcacagga acgaccatca ggagccagcg ttactgcgct gttatagcct
    11161 tccttgacca gggtcttaat ctggcctgat ttaatattca gggttttcaa ggttagccga
    11221 ccacgttctt ctatcagtaa gagcaaacca cgactatcgg gcaaccattc gtaggcttga
    11281 atccggatat cgtttttttc ggtcagattg ataaatttgc cagtagccat ttcatacagg
    11341 aacaaatcgg atttatcggc ttcgaaaccg gcacgtgcca tcgaaaggaa gcttagatat
    11401 ttgccgtccg gtgaaaatct gggagcgtaa tcattgccgc ggaagtcacg gtactcctgg
    11461 gcggtggtaa ttaattttgg ttcgcgcgga ttagcaattg aggctgacca gatgtcgtta
    11521 ttggtgctaa tggcgatgac cggatcgtga ttactattaa aaacgattgt ttggtcatcg
    11581 ggagaaaaac agtagccttc acccagcgct atgggtgggg catcaaaatc gccaggcgta
    11641 acgtctgtta aactaccgtt attcaaatca agcacgaaaa tgtgactacg tttataatcc
    11701 caccactcgt cccaatgacg aaacattaat tcttcataga cgcgggcttt gattggactg
    11761 gcttgccggg tggcgtcgcg ttttttcatc tcatcgaagt cagtcaaatc cggatagaca
    11821 cgggccgaga aagcaatctt ttgaccattg tgcgaccagc agaaactttc taccccgcca
    11881 taaaagtcag taatttgttg aacgtgatta ttatccaacg ccagtttcca gacctgaacc
    11941 tgtccgccgc tatcggataa aaatgctaaa cttttactat ccggtgacca gagtggcgac
    12001 cagctattgc cagaatgggt aggaatctgt tgtaaatttt ggccatcggg gccgatacgc
    12061 cacagtgttt tttgcccttt attggcttcg atatctatgg tctgtacggt gaaagcgatc
    12121 cattgcccgt cgggtgaata ctggggttct gccagtcggt gacaattcag taagtcatca
    12181 atctgcatag agcgcttagc agtgaatcct atatccacca ttatgattag agatagccat
    12241 attaatccga cagcttttga tttcattggt aactccctta tatataggtt ttagatgctt
    12301 ttttggggca taaatagcga taaattgacg ggctgaaaac ggtgattata gatatattta
    12361 ccgagaaaat aaaaaagtgt gccggtgata attccggcgg ccgcatctat ggcataatgg
    12421 taactgcaat atacggtgga gattataatt ccgagacaca agcccccgat tggaatgcgc
    12481 agcttgggtg aatttttaaa agtgtaaatg gcaaccacaa ccgcggcgga aacatgcgtg
    12541 ctgggaaaag caccgccacc attttgttcg gacactccat aaataaaatc cattagggga
    12601 ataaaaacca caccctgatt aaagagatcg ttacggtccg ccagcggtcc atcgcttggg
    12661 aaaatcataa agaatataaa atggaaatac atcataagcg tcagactgaa aatcaattca
    12721 taagcctgag gctgacgccg cccaagcatc caaacaactg ttaaaaagat gactaagtaa
    12781 tatgaaaaat aaaagaaatg cattatttca tcgagccaca gactatcgac cgatgaagcc
    12841 attactttgt tcaaagccga accgaacagc ctgagatccc attttataag tacgggatcg
    12901 aaagtaatgg ggaaaatgag attatcccag gaggtacatt gcgtatatag aaatgccagg
    12961 gatataatcg gccagaaaaa atgaagccag taaaaaaatc gccgctcttc gtgcgtttcc
    13021 ttgaaaatca acattaatag cacaataatt attccacagt cggtactaat ccaggtatat
    13081 ggcttgccaa gccgagaata attggtaatt ccgattaata gcattaacac agtaaaaccg
    13141 aataccaggc aatcggctgg aaatactccc tttttgtagt tgttgattaa gggagtgggc
    13201 gattcgggtg ttagatccaa ttttgctctc ggtaccattg agctgtgcgt ttaaaacctt
    13261 ctgaaagatc aacctgaggc ttaaaatcaa gcaatttttg ggctttggcg gatgtacagg
    13321 tccaatggga ttgattcatt tcgcgatatt tgtcgcgatt aagactacca atttttccag
    13381 tcatttttgc aataatctgg gtgggaacta cgatcatcgg taagataaat tgcggcacta
    13441 aaattggtaa tgtatgatag ccaagaactt taccagccgt agcaataatc tgattccact
    13501 catagccgtc ggattgttca tcgcaggcga aatatatttc accggatgga cggtcgctca
    13561 aagccgcctt ccataacaaa tccaccagat cgccgacata gatcatacca acgtaacgtt
    13621 tttcgaagcc gagatgcgga ttaaaacgcg atttagcgat tttgaagaaa gccagagatt
    13681 ctttatcgcc gggtccataa accacagccg gacgaataat tgtgcaccaa attctgtttt
    13741 tctgctttag aacttcgatt tcggcttcga gtttgctctg tccgtaagct gaaactggat
    13801 aacagggctc agactcattt ttcggtgacc aaggctcaga cggtccactg gcggaaagtg
    13861 aactgacgaa aatgaaccgc acctctttct tgccgtattg cttcaaggct ttcaaaagat
    13921 tgacggtgcc gttgacgtta atccgggtga aagtggcatc atcttgggct ttgatagcac
    13981 cggcaagatg aaagactaag tcacaatctg agacggctga agccagcgat tccggagcag
    14041 taatgtcacc tatttgccac tcgacgcttt gcgcgaattc atccggcttg atactggtgg
    14101 agcggcaaag agcgcgcacc tcgcaatttt ctgcaagtaa acgtcggata agatgctgac
    14161 caataaatcc ggttgcgcca gtgacgagag ctttcatcag accggcaaga ctcctacttt
    14221 ttggtttaca aaatactgaa tcagatacat cattaacata atgatattag caattacgat
    14281 cgtgtaccag aaaaatatca acggattata taataatgcc gacagaattg caatggtcat
    14341 atgggtagaa ggatcgatca atagccagcc gcgcataaca caattgttgt agcgataata
    14401 tctgtcagcc gggtaggtgc ggctaaattt ggcattcggt ttctggacat tgcaatagaa
    14461 taggtaaagg ctagccagca atttatcaga ccagtgaccc ttcgagcggc ttagctgttc
    14521 acgataattc tgaaaatttt gccgacctcc ggccaatgac ggacactgtt cgagagcatt
    14581 gcgctgaaat tcccgccggg cgtgatcgac tgccatcgaa tgagcgatcc acgaaaagag
    14641 actcaatgga ataagcaccc agagcgagaa tccaaattgt gaatcagtac gactgaaacc
    14701 gacggtcata cctatgaaaa tcgcggctcc gttgcaatag tcaaccagac catcaactat
    14761 aaagcccagc ggcgttccat tgtgtttata acgcgccaac ataccgtccg cgcaatccag
    14821 aatgacattg gccataaaca gcaaaccggc gatttggcag atccgcggag tgccgatcgc
    14881 tagacagtag ccgccgctta aaccgatgat aatcgccgaa agcgaaatca tattcggcgt
    14941 aatcggcaag ggtataatgg tcttcaccaa accgaaagcc agcggtcgat agaaaaacat
    15001 atccggccaa ctttcgatcg cgagcggttt cagcattgaa cgatattctc tgtacaactt
    15061 atgccagatt ttcccaggcg atttggtcat aaattcattt taccgatata atccaggatt
    15121 ttatttctgt cgtagaatcc agagtttatt taatagttct tctaataccc gaccgagatt
    15181 ttcgtcgcgg gcagtcgtga tatccggttc attatgtaaa acaacataag gcactttaag
    15241 tcccttaatt ttaataaatt cagggtcatt accgcggaat tcttcccaat cgcaatcttt
    15301 atagagacgg gttaacttcg catccggcag tccatgttcc aaaatgctat ccggcttagc
    15361 gggattaaag cgctggcgat ttttacgcag tgattcttcg aacgatacat caatgtagaa
    15421 aatagccgcc cgctttaaaa tttcatcaga taaatgcgga taagccgctc gataaccgcc
    15481 gtgttcacta ccgcgtgaaa attcaataag agccgtgtag ttcttatgaa aattgggttt
    15541 gtcgcgcaga cgtttactgt attccattga gatacgctca atgagtaaat gccaaagata
    15601 ttcccagaaa aaataaccat ctttgtcgga atgcagtcgc ggtttattca atattttttc
    15661 cagaatggcg tcttcttcga accaggtcca gagcatcgga aaatcatcaa tttcatccaa
    15721 ctcgccaata tgaaaacggc gccggcgtat ttccaccgga gtttttttga gataatcaat
    15781 gacctcggac ttgccggatg ctggtcggcc attaagaatg ataatgtcaa aaatattatc
    15841 cataaaaatg ctccttgatt attaatgggt tgattttcca gtgggagtag tatgccagat
    15901 agaagacatt accagtgcgc ccagaatggc aaagggaatt ggccagattt tccaggcttg
    15961 ccagccccaa ttggtagtaa cccagccgac cagcgcgccg acgaatgctc ccgcgatata
    16021 ctgcataccg tcaattaatc cgacggcggt cgcggcggct ttgcgaccac caaagtccat
    16081 cgtagccgct ccggcaatca taccgtggga accgttaacg aaaaatgaca agcaagctaa
    16141 caaaatagcc gcggcaatcg gtccccattg ccatacgtca gctaaaaaga atagggtgag
    16201 aattacaatc ataccgcaga aaccaattac gatcacgggc gcacgccggc cgccaaagac
    16261 ccgatcggag gaaataccga aggcaaaacc accgattatt cccagaatag caatacccca
    16321 ggcggttaat tgaaaagcga aatctactcc ggtaacaccc tgcatttctt tgaaataaat
    16381 tggccaccag gcatctacca cgccgcgccg tacaaaacct atcatcatcg aaccaatggc
    16441 aatagtccac ataaccttac tgccgaacac tttggtaagc acttctttga acttgacttt
    16501 ttcctcaaga ttaacgtcac catcgcccgt atcgagttcc ggaaagccag cttcggcggg
    16561 tgtgtctcgt acaaagaaaa tggtcaggac gaaaagtagt gctactacta atgccggaat
    16621 ccaaaaagcc caataccatg gcagaaattt tacgatcaat ggtgttccgg aaaaagccag
    16681 tatcaaacca aatcggatta gcacgccaaa aaatgcagag aatgtgcccc gctctctgat
    16741 atggaaccat tgggcattga tttttacgat tgataatgcg ccgaaagatt gaaaatagcc
    16801 attaattgcc cagataatag ctagggtcca gagaagcgcc gatttggaga atccaaattt
    16861 taaaacggct tcggagacaa tctcttgatc agcccagacg gctggagttt ggaccaaaat
    16921 gtggaaaaat ccaaacagcc cgttcataat tacaacgccc agggcaccaa tcaggaaagc
    16981 cttgcggcca ccgattttat cggcgagcgg gccattgatg acgaccgcca aaccataaac
    17041 caagggcata agtgtttcga aaacccctaa attgctttta gtccagccga aaaaagcttg
    17101 gagagtcggc gcaattgctg aaaaattata acgggacata tagaaaaagg agtacaacaa
    17161 gccgatgata atccagtttt gaagacgtct gtgtcgaaaa gtcggcatag ccagtaaatt
    17221 ttcagtttcc gaagtattca ttttgattcc cctatgttat ttttggcaag tattgaacag
    17281 tcatttacca ctaatctgaa attattaatc tatcttcaaa aaccaaactt tatttttcag
    17341 tattttagtg ataatttcca atctatggaa atgatagaag tgaatctcgg ggaaacgcga
    17401 acgattactg ttcagcccgg ccaaaaaata attgaaatcg ttaaacaagc tctgccgcat
    17461 aaccgatata aaccgtatat cgttattcgc aacaatactt ttacgtcatt taacgaaatt
    17521 gtcaacgagc cgactaaaat cagccttctg cgacacttcc acgagtcatc ccggcgggct
    17581 tatgaaagcg cggctattct gattttaaat tatgtggtcg aggatttgta tcccgaccag
    17641 cgtctgatcg tgatgcatag tatgtgcgac ggcgtcttct gccgattcgt gaatgaagat
    17701 ttttgcacgc ctcaaatcgc tcaagaaatt tcagcggctt ttattaaagt ggtagaagcc
    17761 gattatgcga tcgaaccgct tttaatttcc tgtaacgacg cccgtcggta ttttaaagaa
    17821 atcgggcaag aagatactga agagttgctg aaatatagcg aagccaattt tgttacatta
    17881 taccaaatta acggtcgccg tttctggtta ctctcaccgc cggctccctg taccggactg
    17941 attaaagtat tcgaaattag accctaccaa aaaggattcg tcctgcgttg tccgaccgaa
    18001 ggagatgatc gtcgtctgcg acctttcacg gatcaggcgc gactgtatga aatttttcag
    18061 gagactgagg aatggggacg cattttagaa atcagtacgg tagccgatct caatcgtacg
    18121 atagtcgaaa atcagatttc ggatttaatc aaaattgatg aagcattaca cgagaaaaaa
    18181 atagccagca ttgccgacca gatttcaaat aaaggcgagc gccgttttgt atttgtagct
    18241 gggccatcgt cttccggcaa aactaccttt gccaagcgcc tcgctattca gttacgggtt
    18301 ctgggattca agcccctttt gttgtcactg gacaattact tccgtgaccg ggctgaactg
    18361 gccaaacttc agggcaagcg aattaattat gaagtgctgg atgcgattga cctcgagctc
    18421 ttgaacgatc atctgcaacg acttttgcgg ggcgagacga ttacaccgcc ggtatataat
    18481 tttattaccg ggaccaaaac tcccgccacc aagcccatga aaattgattc ccaaacgata
    18541 ttaattttcg aaggaattca cggcattaat ccgggcttga ctcctgaagt taatgaacgt
    18601 tataaattca aaatttacgt tagtgccctt actcacttga attttgatca tatgaaccgt
    18661 gttcgtactc atgacacccg actattacgc cggattgtac gcgattataa atatcgcggg
    18721 tatagtgcgg ctgaaactat cgaaatgtgg aagaaagttg tcgagggtga gaataaatat
    18781 attttcacct tccagggcaa cgccgatatc atgttcaatt catcgctggt ctatgaacat
    18841 ggcgtcttga aaaccttggc ggttaccatg ctgaaaaatg tgccggaaaa tcatcatagc
    18901 tatccggaag cacagcgctt attggatatg ctgtcgtatt ttctgccagt ggacgatact
    18961 gaaattccgc cgacctcgat tatgcgggaa tttatcggta aaagttcctt tacctattaa
    19021 tatgctgaga ccgaccggat cggtgcccaa cggcgtcaga tttatcctaa gatcacaatg
    19081 aattataaga taatcttaga agggaatttc atcatccgga gatggctcgg gcggcgtttc
    19141 tggttcagcg gcttcttttt cgcgtccgcg tccggaacca cctagcatag taatttggtc
    19201 tacctgaatg tcagttttac tacgtttctg accttcttta tcttcccatt gacgggtttg
    19261 gagacgacct tcgacgtaga ccagctgtcc ctttttgagc cattccttgg caaattccgc
    19321 ctgattgcgc caaatcacta gattgtgcca ctcggtgcgg tcttcacgtt ttccgtcacg
    19381 gttttttacc gattcagttg ttgctaagga aacctcggca actgcatcgc cgttcggggt
    19441 gtagcggact tccggatcgc ggccaagacg accaaccagt atgaccttgt taacacttcc
    19501 ctttgaaaga gccatcgtta atcctccttt ttgttaaaaa ttaaatcctc aaaattggga
    19561 ttgcaacttc cgatgctaat tctacaaaaa acttataact ttgtcaaaca ctatttttat
    19621 cccttattga gagattttct tactttcgta aaagccgcca cggctcgatc aatctgctcg
    19681 ggagtgtgag ccgccgaaac ctgaacgcga atgcgggcgg tgccgcgcgg taccaccgga
    19741 taggtgaacc caataacata aatgccttcc tcaagcagaa gatcggcgat tttaatggcg
    19801 agaggttcat cgtaaatcat aatcgggata atggcggtat tgccggggat aatgtcgaaa
    19861 ccggcggctt cgattttctg gcgaaaatgg tgtgcgtttt caatcgtgcg gcgtggcaag
    19921 tccggatttt cctgcacgat ttccaaagcc tttaaagtag ctccgataac ggccggcgcc
    19981 acggaattcg aaaaaaggta aggacgcgaa cgctgacgca taaattccac aatttcccgc
    20041 cgcccggaaa tgcaaccgcc agaagcgccg cccagcgctt tgccgaaagt agtcgttatt
    20101 atatcaattt ttcccaacaa gccgtagtat tccgctgtgc cgcgacccgt tcgcccgaga
    20161 tagccagtgg cgtgcgaatc gtctatcatt accagcgcat cgtatttttc agccaattca
    20221 cagatttgtg gcagaggcgc aatgtgaccg tccattgaga aaacgccgtc gctcacaata
    20281 atgcgatatt ttgcctgagc agaattttcc agacattttt gcagttcatc tatattcata
    20341 tgggagtaac gaaaacgctg agccttgctg agccgtattc cgtctatgat tgaagcgtga
    20401 ttcaattcgt cggagatgat cgcacattgc tcatcgaaca atggctcgaa aacaccgccg
    20461 ttagcatcaa aacaagacga gaacagaatc gtatcctcgg tgccgagaaa atccgataac
    20521 cgccgctcca attccttgtg tatttcctga gtgccgcaga taaaacgcac tgaggaaagc
    20581 ccaaaacccc agcgctcgag agctaactgg gcggattgaa taataaccgg atgatttgac
    20641 aatcccagat aattattggc gcagaaattt agcaattccc gaccatcagt cagccgcaag
    20701 tggggcgctt gcggactggc aatgacgcgc tcggatttat aaagcccctg tttagttatg
    20761 gattcaagct ggctctgcaa atcctgttgt aatttgccgt acatataagt ccttataatt
    20821 tagttgataa actgataatt gacctcgctg gcgagaagca ttttgtttaa ttgctatggt
    20881 aattcgacta tggcagactg gcgacacata taaatttagg tgataaaacc gataaagtga
    20941 aagctggtag agtgctgacc gcgatacttt gaatttagaa gggattttca tcatggaaat
    21001 ggagtttttt tgacattgag aagtttagtg tagcgatttt ttcaaatact ggattttaag
    21061 cctgatttat ttgatggctt tatgaatagc ggccgcgccc aaaaacatcc gcttgaaacc
    21121 gatctcgcgc aaaccagcct gacggatgat ttcagccaat tcttccggag agtaaaagtg
    21181 gcgaatagaa tgggaaagat aagaataagc gttttctgaa cccgaaatca agcgaccgat
    21241 tggtttcaca gtggctttga cataaaggtg aaatagtcgg cgaatcggcg tccatcgggg
    21301 ttggctggtc tcaagattca caaataagcc accgggtttt aaaactcgtt gaaactctcc
    21361 taagcattgg tgaagattat cctgacttat actgaggttg cgagtggcaa aggatattgt
    21421 aatgacatcg aaagtgtccg actgaaaagg taaacatttc atattcgcca aggtgaaaat
    21481 gatgtgcttc gccccggact tttccctggc tttgcccatc atcggaaggg aaaaatcagc
    21541 tacaaatata gacgtgccgt aatttgctag gcgggaaagt tcgatagcca tctcgcccgt
    21601 gccgccacag gcatccagcc atcgccttcc gcctgcggaa gtggccagtc gggcggctct
    21661 tttgcgccac acgttatctt gcccgagagt caaaatttgg ttcagaattc gataagttgg
    21721 cggtacggtg gtgaacattt cctgaatgga tggattcata aggaaattga tggttttatg
    21781 ccgtagaggt gcgttgcaac gtgcctctac ggcttgttaa aatttttcta ttatttcaat
    21841 aaagattttt gaagataggt atccatcaac aatttgaaca tcttgccgtg gttgggatat
    21901 tttaagtgca gcaattcatg aagaatcacc ttcagcctaa cctcttctgg ttctttcaat
    21961 aatttcttat tgaacgtaag tcttcctttg ctcgagcagc ttgcccattt gcgtttcatc
    22021 ggctgaatat ggatttctct tggcttcact cccacatcat tcgccagtgt cagcacattt
    22081 tccttgaatt tttccggcgt catcattctt ccctgtttgc tttcagcgtc ttcattatct
    22141 tcttcacaat ctcaggaatt tccttaacat cctttacgcc tgaacgcgtg atagtctgat
    22201 aaataccggt cctgatttct ctctcgtact tttcgctttt ctgccagtgc ggatatttct
    22261 cgaacagatt cctcatctca tttgccatcg cttcggcatc tttgaaacct tcgctcttca
    22321 gcatccagta aattgtgaaa ttctcagccg acatattctt ttcagtctgt tctttccttg
    22381 catcgttgat ttcttttatt atcctcttta actcttccag cgtctcctgc gtattctttt
    22441 gcctcatctt gtacatcttt acaagaagtt ccgctttctc ggcaatcgaa aaaagatatg
    22501 gcgagtttcc actttgctcc tcgactgttc tctcgatgct cttgatgaga ttgaatactt
    22561 tttccgtttc agaggctttg ctttcttcga ttcttcttaa agtcgtttcg ttgatttcac
    22621 aaacttccaa cgtcggtctg atatcgctgc tcgtggtatg cttctgtaca agaagagcgg
    22681 tttttctggt aaacatttta tcgatcagag tccctctctc ataattttcc cttacaattt
    22741 tatacattct taaaagcgtt tcgtaatcgt ccagataaga gcgcaaaaac tcatccggtg
    22801 aaatgatctc atagatatcc gacaactttt tgaaatactt atagaattcc tgtctctcct
    22861 cttcatcccg gaaatgctcc agcgccgcct ctaccgcttt atcctgcgtt ttcccttcta
    22921 tgagcgtgag atatttttct cttccgagtt tcatttcgtt ttcaaatcta tttttcaaaa
    22981 cctgaatatc gcgtacaatg ccttcgatgt ctttcgagtc gaacgcaagc gctttttcga
    23041 gattgtcgaa gatgccaaca aaatcgagta caaagccgga ggactttttt cttccctcat
    23101 catcttcata cggacgatta actcgagaga ttgcctgtag caggacatga tcccgcatcg
    23161 gtttatcgag atacatacaa tagagaatcg gcgcatcgaa accagtaaga agtttttcgg
    23221 ttacaatcag tattttgggc agttcgttcg atttgcgaaa ggcttttcgt atcctttttt
    23281 cttctatttc cgaaagttga tatttagcca gttcaggtga atcgttgaaa gcggagctgt
    23341 acactacctc tgaatattcc ggcggtaaat atctatcgag ctcttctttg taaagcgcac
    23401 aggcttcccg atccaccgca accacaaaag ctttgtagcc catcggttcg atgtaatttt
    23461 tgtaatggtc gcaaatatat ctcgcaacct tttccactcg ctctttgttc ttaagcatat
    23521 ttttgagcgt tactgctttt tccaacactt tattgagcgc ttcgatgtcg ctgatgcctt
    23581 cagcttctgc aagattcaaa aattccttct cgagcgcttc cttctccact cgcaattcgt
    23641 tcggagcaag agtataatag agcgggactg ttgtgccgtc ttcgatagat tctgcaatgc
    23701 tgtatttgtc gagatagccc ttcggcggat catccttgcc aaatgtaaca aatgtgccgt
    23761 tgccatattg cgttttgtca attggtgttc cggtaaaacc aatgtaggcg gcgttcggaa
    23821 gcgctcccat caaataatta ccgagctttc cgccggtggt tcggtgcgct tcatccacca
    23881 gtacaaaaat attcttgcgc gtgttgatgt ttgccggtat tccctcgaac ttatgaatca
    23941 tcgtaacaat caaaccgcgc cgatcgctgg aaagaagatt ccgcaaatgc tctttgcttt
    24001 ccgtaacctc gacgtttcca atgccaagcg ctgcgatgtt tccaaacagc tggctctcaa
    24061 gttcgttcct atccacaagc ataataaccg tcggattttc gaagagcgga tttgcaatga
    24121 tcttctgcgc tgcaataatc attgtgtaag ttttgccaga accctgcgtg tgccatacaa
    24181 gtccccgatg cttttctgga tcctttgcac gttcaacaat tttgtcaaca gctctcattt
    24241 gatgcggtcg aagaataact tttagaaggg catcgtcctg ccgcgtgaac atcataaaat
    24301 caacgagaag ttttacgacg cgttctctat cgaaaaaagt tttaacaaga tgttcgaagt
    24361 cgccgccgga ctcttccttc cagttaaaga gaaaacgttc ggatgtattc cacgtgccgc
    24421 tgtagtaata ttgtacaatg tgagtcaatg catagacttg cagaaccgca agaagctcgg
    24481 gacattctct gtgataccgc ttgacctgct ccagcgcctc gctcattcct tctattttat
    24541 cggatgattt tgtttcgatg aacaaaacag gaataccatt gatgagaaag acaatgtcca
    24601 acctgatggt tttgattccg ttcgtaaact taaactcatc ggtaacgtga aaaacattgt
    24661 tgtccagatt ttcggtatcg agtaatttga tgttacgttc ccgtttttcg ccagggacaa
    24721 acacggtttt caaacccttg agatattccc atgcgataaa attcccttcg atgttcgatg
    24781 gtacggattc gatttttttg acaatttctt cagccatcga ggaatcctta aaatctggat
    24841 tgagtttttg aagttgatcg ctgaagactt ccttgaacac cattccggtt tctccgcctc
    24901 gcaggcgaag tgcttcatcg ggagtaacgt actgccagcc gagctttaga aacactcttg
    24961 cgccattttg cgcaacatat tctgccgatt gttcccgcac ataatctatg atgggctgtt
    25021 gaacggaata tttttcacct cccaatttgc tcatgatgtt acctctacat tacggttaaa
    25081 aagttttgca ccgctttcag tgttcagtag actgagtagg ctttcagcgt ctttgatctg
    25141 gcgtttcagc aactttattg tagactctaa ttcattgtcc ttcagagtgc cattctcgtt
    25201 ataaaaaaga gcaatggcaa aatcgaagcc ctgattatga ataaaaaatc tgttgggtat
    25261 tttctctata ttacctgcaa tcaacccgta tcgtaaatag ggatatattt ccttgtgttt
    25321 cattgcctta gttgaatatg tgaggatatc gtgagtggtt ataccgagaa actttatctc
    25381 gatagctact atcggaatat ttttcttctt gataagcata tcggtctgga atgcatagct
    25441 tcctctcaaa gggtttttaa gttcatcggg cttaggctta acatcaccat taccgtctat
    25501 aataatctga tacagaagat ttgtttcctt tccacaatta caaaccgtgt atcctgaaaa
    25561 ttcgctcttg attttttcat agagttcgtt tcggaaatca ctttcagttt ttttattacc
    25621 ttttttcata gatcaaatat ccttttcatt caatttaagg ttattcactc ttattttccc
    25681 ggtcataagg ttatgtaaaa ggcttttgaa caattcctcc agcgccttct ttttattttc
    25741 ctcagcttcg attttagcat cgatggccga aagaatcgat gcaattttct gctggatagg
    25801 aagaggaggg agtgggagag aaatttgttt tattatttgt tgatttatat ttggttgagc
    25861 tccgccaaac cgcatagata aaagtttatt tcttatcatt tgaaaataaa aaaaataaaa
    25921 ttcaggccat aatttttcat taagattaac tgcgcatatt gcctgattaa ttgtaaattt
    25981 cgcttttgcc aatcctactt tgccaacagt agcaccatac attgcaatta aaagtgtatt
    26041 attttcaaca tattttgcag aagattttt
//
LOCUS       DAOOMU010000021        25751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1780_length_25751_cov_22.849821, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000021 DAOOMU010000000
VERSION     DAOOMU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 25751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25751
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1780_length_25751_cov_22.849821"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..296)
                     /locus_tag="PLI55_03355"
     CDS             complement(45..296)
                     /locus_tag="PLI55_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99907.1"
                     /translation="MKLDEELLKEICEYMGSDLNSAPCKIIRDHMEACPNCEVYIDKI
                     KRTIEIYRLADQCEELPEKVIRNLLTCLKLDDPSKTCKK"
     gene            complement(293..883)
                     /locus_tag="PLI55_03360"
     CDS             complement(293..883)
                     /locus_tag="PLI55_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPA99908.1"
                     /translation="MENNLPEEELVARAKKGDQAALSQLVLNYSSRIYHLGLRLTGSH
                     QDAEDILQDTFLIMVKKINQFQGKSSFYTWLYRIAMNVGLRKLKSKPRKYEHISLDDP
                     DFERVSSEAASEWPDFGYDIVNDPHFRQRLDRLIEQLPEKYRTVFILRDLHGLSTEDT
                     SRILQITPSNTKIRLMRARNFLKENLEKMLKTEMQL"
     gene            complement(941..>1558)
                     /locus_tag="PLI55_03365"
     CDS             complement(941..>1558)
                     /locus_tag="PLI55_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPA99909.1"
                     /translation="REVFSRLNNPVKIINFTQTLECQYCRETRELLEEITALSDKLSL
                     EVYNFQIDRSVAERYGVDKIPATILFAETAPHSRIKFYGIPAGYEFAALLEDIVDISN
                     NKTDLSPEILAGLKKVTWPVHIQVFSTPTCPYCPAAVRTAHRFALLNENIRADMIEAI
                     EFPHLAQKYNVRGVPRSVINENIIIDGAVSEKQFLEKILSSQPTT"
     assembly_gap    1559..1658
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1659..2350)
                     /locus_tag="PLI55_03370"
     CDS             complement(<1659..2350)
                     /locus_tag="PLI55_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPA99910.1"
                     /translation="MDIVALARKKGLLFDGAFGSMLIQHGLPTNANAEVWCLEHPEVV
                     TKIHAEYVAAGADVLTTNTFGASSLKLQKAGVEKRVAEINHRMVEMARAAAKPNTLIA
                     GNIGPTGEMLAPFGKISIEEATINYAEQAGFLDKAGVDLFIIETMFDLNEALAAIKGI
                     LQVSAKPIFATMTFSESPTGFVTIMGNPVEESMRQLVEAGATAVGANCSIGSEKMVSL
                     AQIIRAAVSVPVV"
     gene            2699..3271
                     /locus_tag="PLI55_03375"
     CDS             2699..3271
                     /locus_tag="PLI55_03375"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HPA99911.1"
                     /translation="MTKIIRCPWPANDELMIHYHDTEWGVPLHNDQKLFEFIVLDSFQ
                     AGLSWRTILHKRENFRRALAGFEVNQVANFGEIEINSLLANAGIIRNRAKIQATINNA
                     ARFLEVQQEFGSFDRYIWQFTAGKTIVNHWQKLSELPDKSLEAEAMSADLIRRGFRFV
                     GPKICYAFMQAAGMVNDHLESCFRWQELQG"
     gene            3536..4144
                     /gene="rdgB"
                     /locus_tag="PLI55_03380"
     CDS             3536..4144
                     /gene="rdgB"
                     /locus_tag="PLI55_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004092423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPA99912.1"
                     /translation="MDILIATHNRDKVREIKRIFSLPELIWHTLDEYPDCPKVVEDGN
                     TLFENAFKKARMIAEFTGLPTIADDTGLEVDALHGQPGVFSARFAGENATYAQNVAKL
                     LEVMRDIPDSERQAHFRCVAVYYSPDLTLVEEGKIDGIILRQPRGNGGFGYDPVFYLP
                     QLGRTFAEMDLSEKNSLSHRGQAFTRLANKLKSVDKFVTDNI"
     gene            4300..10575
                     /gene="sprA"
                     /locus_tag="PLI55_03385"
     CDS             4300..10575
                     /gene="sprA"
                     /locus_tag="PLI55_03385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPA99913.1"
                     /translation="MAADREPDVFPLKIDWLSNRYTPANIESKRGLSLISPDYATLNQ
                     NILKVKFPGMKVTNTLDKSGQNFIFYNDLNGAPVLTPIGIPVASYFDLRQRLNWHNKL
                     VEVVTAKPADTKAASDKLELIGADIAGQHVALRVSGNININGRLQTQKRSQVSSSYAE
                     GTSTSFIVDQKQQLNIEGKIGDRISILVDQDSERDFDFENAMKIIYTGKEDEIVQKVE
                     AGNVALSLPGTQFVTFSGKNNGLFGLKALMKFGGVDVTAIASVEKGKKEKLSIDGGAQ
                     TSNISIKEYEYRRNLYFFLDTLFRDNMYRGYQFDQLFHADPRGKVTDLQVYKSQTIET
                     AGCAYGTAYVDPRDTTLYKDYSENRIFKLLELNKDYYVDVTLGYIRMTTQVQDGEILA
                     VAYRLTDDNNVTVTGEEYGDYNRSDTSTAHIILKLIKPQEMLPTHPCWNLEFKNIYYL
                     GTTGINSDGFELDIVYTLGEGEQNYDLVTGKKFLEMFGLDRVDNNGNPTPDGVIDMNN
                     GAILNLYSGELWFPYLRPFQYDSTDNAGKRNPNISKIYNCSAMYDSNRTDNDIFSETK
                     FKIMVSYKNRSSILNLGAMVIENSETVTLNNRDLVRGVDYIIDYFSGTLTLLTSEATN
                     PDAKLEVKYEKNQFFQLDKKTILGARAQYDFGENSYIGATALYFGQSVSDEKVEVGYE
                     PMHNFVWDINGRYNRNLDFLTRAVNWLPLVSTNAASSFDIEGEVAQVLPNPNTLSNEE
                     TGDRHGVGFIDDFESSKRVTSPPIMRRYWSNAACPLGKDENERGFTFWFNPYGGVPTK
                     SIWPDKEVSTTAQNNITEIMVMSVDPNWSIAVVDGVSPVQSAWGGITYAFPQSYYDQS
                     TTKYVDIWVKGNSGTLHIDLGQISEDQVPNKKLDTEDIPDAGLTMGNGLLDPGEDTGI
                     DGLVDKNEKIVTTKYGELSYGDTRLTEYKRDISDPHSDNWKYTEGSKEYRYINGTENN
                     EKQTDGLFPNSEDLNNNYAIDLTNDYFTAAFDLSDPNNEYIAGKTYIDSRPTGWKLYQ
                     IPLTAFSKSATGKDISWESIRACRIWFDGLTGEDSVFIAKVELVGNEWEDLGIAKDDT
                     SIFVVNENSFSISVINTEDNPDIYEPPAGVQGEYDEINQIRTKEQALVLNLPEEGSGL
                     FPGEFCAAKKVLAEETSYITYKVMKLFVNGHELKADGYQFSEGEETPLQFFIRFGKIF
                     ENSPTTTNYYEYRQPIYPGWAEENEMELDLDFLTSLKNYESADDYIGDGEYRIYTNTS
                     GVTIRHYKEKFNDDYTGREIIIYGKPSLANVKHLNIGLRNRADNSGYVNKFKTGTIKG
                     QVWLDELRLSEVRKDKGIAYRAKASLRVADLASFDVSVNYRDADFHTVEQRPSLQTEN
                     LKTTEALTATGRISLDKFTPASWGLRLPVSGSYTQTTGTRKYIYGSDILMKENAPDSL
                     LDISHSYGVNTSIEKRASDFWLTKYTIDQIKISANATWTDASSVTVRESQSESYKASI
                     SYNFPFPKDKYINIFKWTAPVPLIGNKLSEIRFYYFPNALTVTADAGRSESYSTPRYG
                     TTITSSNSFGLNRNLSLGYKIFDNLSANYTLNLENDLIELWQNQWEILKSANPGQITN
                     MRESYSVNFNPKIAKWFNPTFNYTSNFNWNEPIVSNSASVDAISNQNRLTSTFSLDPS
                     AIFSSLFKPSGTAPAPSPTPTPPGQTTGGRRATQTGALNQPKTAEPAPITGDSTRTDQ
                     KSGEKRPRKPGSTIDKGEDLKPTSGQTLEQMPKPVDDVKKPEVNITQIVAKYLKKIQP
                     FNFNLSLNNSVTNRYRTGTPDWQYRVGLTRDPGIPFVMEEYIPTSAENTSNSFDFSVR
                     SGINLFSNVVIPLSFAQNVSNTQSQSTVSRTLTRDYIPSGINGKDGFPFPSWSLTWSQ
                     LEKYQIFSKYFKKLSLDHGFSGKESIITQNGKEMSSSYKRYFQPLIGLSMQFVNDISA
                     SARVTSGQSINNASGGTTIIDDAGASISLSWQKKGGLSIPLPFMKDKQLKNNITFTAN
                     FDYTNSTTRAKQGSATKFAVTAKSNSWKTTPRINYTFTDKVTGGLFFEYGESYNRLTG
                     KRVTRNYGFDVNIAIRG"
     assembly_gap    10721..10820
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10821..11318
                     /locus_tag="PLI55_03390"
     CDS             <10821..11318
                     /locus_tag="PLI55_03390"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPA99914.1"
                     /translation="AEILSRQQPPRTIYLVFFDGEDYGFPGTLDYYCLGSEYFAKNLP
                     IPKPEEAIIIDMIGDADLRIPVERNSVKSHPQLIKKLWAIADRHNFSAFVHQIDEEIY
                     DDHLMLIQHAGIPSVDLIDFQYPNRFINYWHTTLDTPDKCSAESLEIVGTVLYDYIFY
                     GSEER"
     gene            11324..12148
                     /locus_tag="PLI55_03395"
     CDS             11324..12148
                     /locus_tag="PLI55_03395"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPA99915.1"
                     /translation="MIATSQRWLKVLIPKSAGNEELIIARLIQWGMLGLQETDTDYEI
                     YFPLNMKLSLPELIAAEWPQLNITITEIVDNGWSTRWQEYFKPQTISPRIAVRPYWEK
                     QLKDVSVEIVLKPGMAFGTGTHPTTHLALLMLEKYLTPGDVLLDAGCGSGILTIAALK
                     LGAAQVSAWDIDSLVIDNFNENLELNGLSRKVAIQIGDVTALPNYNYDLIVSNIERQS
                     NLRLLEALVRQQNRPLVIFTGLLKEENEIFTNKVLNYGREILDQDTEDEWLALVIK"
     gene            12149..13216
                     /locus_tag="PLI55_03400"
     CDS             12149..13216
                     /locus_tag="PLI55_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99916.1"
                     /translation="MKRIIIAALFITILTLWTCENQSIVETVEPTPIIENLTLHYSEL
                     NHSLFIAAKVEDAQGWDNLDSVVFNLYRINTIGSMNGFLFKSGKLSDDGPPRDIITQD
                     GVFSYLIDSTELGSYEGYYRVFVQAYDADGNVSDSATATELVQANSPPEIFLLSAPTT
                     FEKWDTLKFYFSVTDKQGYDDIVGVYYSILRPDGQFFDPYFYLNDAGEYGDEKARDGI
                     FTVFQPSNPESDLQGLYTFYFFAEDYSGAMSDTITLKIPNPGIHITAPASALTLRFGQ
                     TLRIMWEYAYISQVKIEYTFNANETNPDWIVADTTAAALKYYDWTVPATQVTNYCKVR
                     VSDLTKPARYDVNDDYLVITQ"
     gene            13213..14784
                     /locus_tag="PLI55_03405"
     CDS             13213..14784
                     /locus_tag="PLI55_03405"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPA99917.1"
                     /translation="MKKYPAILFIIGFLFTSGFSQAFRLAKPTLDSDNTFLGLASNSV
                     TEIVSQGNSLMWFATGGGLSMSPDFGKTFVSFYPGLNNLPRGGISAIAVKGDTIWVAA
                     VFDSLIQSYGYQQTGGGLAFSIDKGKNWTFVPQPIDDPADNSAEWNNQSVSFLPVTTP
                     INNTTWDIAITDDYVYIVSWAGGIRRSADMGQTWQRIPLPSDDLNNLDCDDLIDFEIN
                     PRDPPDGNHNHKGFSILAYGDTIWIGTADGINLGIVESPECISWRKFNAQNSAISGNF
                     VVAIDRQLWKGKETIWAATLTVGESGEYRAVSKTSDGGLTWTTTLVNERPYNFAFQDS
                     IVYVCTERGLFKSIDGENWAIYTPIADIASGEQILTQYFYAAEVDRREGPTYLWVGTG
                     DGVAKTASDGLTWTVYHSAVSTKSPGQPAIYAYPNPFAPRYQNVIGDDGHVRIQYNID
                     QPASVRLEIYNFAMEQVYRSDWKTVMDAGDHHLIWNGRATNGEIVANGTYFCKLIQKS
                     NGNEVEHWTKLIVIN"
     gene            14787..15713
                     /locus_tag="PLI55_03410"
     CDS             14787..15713
                     /locus_tag="PLI55_03410"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPA99918.1"
                     /translation="MRKFLNLILSLIFSMALLQGETNDNAGYAGAFLRMGLGARATAM
                     GNSGVAFPSNGFGIYYNPAGLAYMDERNLALSYSFLSLDRQFHFIGLSVPLPPNAGVG
                     VSWLHAGVKDIEGRNSIGQVDETYQTGENVVLISFANAFHPRISLGINFKILHNQMLD
                     VSATGLGFDFGVLYQPLEWLTAGLQFKDIGAGYNWNTQDLFGEEGSSYQENFPQILKF
                     GLAIKPIQELTFVGDIGVSDQNVWQTHCGAEYVYRNLAFLRIGMNQLSPTFGAGLAYG
                     FIANMTTQLDYSFIIGAVGEGTTHIFSWQFQF"
     gene            15722..16627
                     /locus_tag="PLI55_03415"
     CDS             15722..16627
                     /locus_tag="PLI55_03415"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPA99919.1"
                     /translation="MRQKLRTGFIIIITGMFLQAESGESGMAFLKIPVSAQTASTMGV
                     FAQVSGTPFTIFDNPIGIINDDTKIGFSHNFWFADVSGDALAATLPLKKGNLGFGLNF
                     VKIPGIEIRDIPSDESLGEVEAQYMSAAVGYAYPIFSKLSIGCTVKYLYEHLYAENAG
                     GIAFDVATHWHIPSGMDLALSLNNIGQMQTLKDKATTLPTLFKIGIVRPEIFADSPFN
                     VNLGINLIANLVTEKTYFQVGGEVAFTNLIKIRGGYERVGTINRTALGAGLNFGRFEI
                     DYAFIFMPDGLGYPNLLTLSYRPEL"
     gene            16826..18214
                     /locus_tag="PLI55_03420"
     CDS             16826..18214
                     /locus_tag="PLI55_03420"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPA99920.1"
                     /translation="MSDRSLLLINPWIADFTAFDLWSKPLGLLYIAKFLQNYGYEIEL
                     LDLMDRQRWQDTSNDKFNDGRGKYRKTIIAKPPVLARYPRRFGLYGASREQFSDALSN
                     MALPNAILVTSLIAYWYPGVAATVEILRRYFPETPLILGGIYASLYPEHARNSIQPDF
                     LITGYGEKKTLQLVDQLFGIERDYNVIPDYNDNGILPWRLYQKLKSVAVLTSRGCPNR
                     CTYCATYLLNKKFVQRRPEDVISEIISTYNQFNVENFAFYDDALFAHRDRHIIPILKG
                     IISAGVKINLHSPNGLFAREINSELAELMFSAGFKTVRLSLESTSSQIQKSSSNKVNN
                     ANYTDAVNNLAKAGFARNSLETYLIMGLPGQTRQQIIDSINFVADQGVIVKLASFSPI
                     PGTTEWERARALGYVSDDQDPLMTDATIFPFLSRNLSYEDYLALRQVANERNAENRSN
                     NQISNEASDGTD"
     gene            18201..18755
                     /locus_tag="PLI55_03425"
     CDS             18201..18755
                     /locus_tag="PLI55_03425"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPA99921.1"
                     /translation="MGQIKSAPQGHLFLAIMSASEHQRQVALEEVRTRFGEIMGAGPL
                     FSVSDFTNYYESEFGRDLKKQFFIFKQPTSLENAHQIKVWTNQIESQFVDNTGSQSHR
                     QVNLDPGYLEPSKLVLFSTKNFSHRIYCGDGVFAEVTLIYEHGQFKILPWTYPDYYWT
                     KNLEFLIQMRNEIVRLSRKDSKQA"
     gene            complement(18914..19561)
                     /locus_tag="PLI55_03430"
     CDS             complement(18914..19561)
                     /locus_tag="PLI55_03430"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPA99922.1"
                     /translation="MNKAFTFLDLTHTLENGMPTFPGDRPKPRIITRSKTQFTISEIH
                     LGTHYGTHIDAPRHFLPDGKSIADFNVDRFTGAALGLHRDSAGTSALNLNDEEIELIQ
                     NLKPQWLILRTGFDRFWSQPSYFLEHPYLSVELAQQLIDLKIKGIGGDFPSMDAANAA
                     RQNFPIHHLLMEHEVLIIENLTNLGALPTQELFHLIALPLKIATDGAPARVVAWF"
     gene            complement(19592..20608)
                     /locus_tag="PLI55_03435"
     CDS             complement(19592..20608)
                     /locus_tag="PLI55_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HPA99923.1"
                     /translation="MLEVIKSTIMITGFVFTMMLVIEYLNVQTRGKWHKLLKGRKWRQ
                     YVLGALLGIIPGCLGSFAMVTLYTHRLVSIGAVVTTMIATSGDEAYVMFALFPGKALL
                     LNVILAIISIPVGVLADLLFRKKYDVVTAHDLPLHDEYCECFQRGKILEQLRSITLER
                     FLLILIMAAILLAAASGFIAGEEEGWVRITLIITSIVGLAIVATVPDHFLKEHLWEHV
                     TKRHVPRIFFWTLGALIVIQILTNYLNIENLLQLQQARVLVLLVACLVGIIPESGPHL
                     IFVTMFAEGLVPFSILLASSIVQDGHGMLPMLAHSRRDFVRIKAVNFGFGLLVGLIGL
                     WLGF"
     gene            complement(20619..22826)
                     /locus_tag="PLI55_03440"
     CDS             complement(20619..22826)
                     /locus_tag="PLI55_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPA99924.1"
                     /translation="MIQNHEKHFPKKFLGNIATLIIGLVVLVAAVVFGFFSQPKEFGN
                     VDIVLDCLSQDVEILTGVDGIAHISAGNDADLLRTIGYLQATRQLPNLDRLLRLATGQ
                     MSAAHGKNFIELDIAAHQLDFVALAEQTLAELSPEAQTLFEAYCAGINAFIATQRHSV
                     AQQFRLAGYEPHKWEPAECLAILQLFKWAYLSQWNEKIVIYKTGEVFGEERTRDGFPL
                     IEPSTVRESGYQNAFFASLNQLYRDGVNLRRTMNLFPNKDKSLAWILAGKNVPADQTR
                     LIFENNWFNSYDFIFDMAVLDRRIVGLFFPGVPICLAGCNQQVAFGTNWLPENEVNLL
                     VVRINPASEQYLAQDGWHNLRKRSTQIAVRSDHKQEVTFYHTDQGVILDYPIESVDTV
                     AYAIVLKWQAVTASEINQRVALMLIGNNDAARVMTEVSSNLPGLVYLDQKGNYGSSAL
                     PKPLTPNLSINCLTPFTIDDWRFSSQFTIDSLKSHFLPDWQDQPKALFSESTDEFFPI
                     LATDKYFARLMKDVRTALPDTIFTRKTELQAYQNLIEWDSRYAKTAVGPTIYQAFLKA
                     LMRNIYSDELNLVDSETFEQFANSGDFAMQNLLLLIEKGESNWFDNLRTSDFVEWLGE
                     IIRQSFREAVQLIEEKYSPNLSEWQWGRVNQDFVWGNNIKGKKFSNSKSVSLNSSGVV
                     QTIILKPAGSETTCCIAVNGTTNPVEPVNLLTDRTELLRNGAHIMTLKASPKK"
     gene            22932..23225
                     /locus_tag="PLI55_03445"
     CDS             22932..23225
                     /locus_tag="PLI55_03445"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99925.1"
                     /translation="MSELIKKILLASVGAVSLTKEKAENLLEDLVKRGEIAREDKTSL
                     LNEILKSIEQSQQETRKFIQKEMEKALKALDIPTRSEFEELKKQVKSRQAHKD"
     gene            23234..24913
                     /locus_tag="PLI55_03450"
     CDS             23234..24913
                     /locus_tag="PLI55_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HPA99926.1"
                     /translation="MRRFSVSRTWRHLKRYNQVLSVLIKYGFSDIVDAARKDLIARFG
                     EKFIPRGRRNQFSGLSHAERIRRALEELGPTFVKIGQVLSLRPDIIPAEIAAELAKLQ
                     DEVSPLPYDVIANVIQTELGSEPDTVFQDIQSEPLAAASLAQVHKGVLISGEPVAIKV
                     QRPEAESLINTDIEILYDLASIIQNYFLENFALDILAFLHEFDRSIHQELDFVLEGRN
                     LRRFRKFFAGDEAIFIPNYFAEYSTSKLLVMELVEGIKATEIEQLEAAGIDLQVIAQR
                     GVEASFRQVFEFGFFHADPHPGNIMILPGNIIAPIDFGMVGQIDEDTIDNLGHVVVGV
                     LHKDITRTLRSLEDLTGADLSAVAHDLRYDLMTYINNYSELPLKDLQLSLLMDDFFEL
                     IRKYHLRIPSHLALMLKALVTVEGLARILWPNFDVLEALKPYVRKITLRRYNPRRIMR
                     NGTLVLDDFADLMTDLPGRLQAIFKKAAEGNFAIQFQHRNLEHLIDEFRKAANQLSLA
                     LILAALVVGSSLVILTMAGPKLFGYSLIGLLGYVAALLIGLRMLWNHFRKK"
     gene            24934..25590
                     /locus_tag="PLI55_03455"
     CDS             24934..25590
                     /locus_tag="PLI55_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPA99927.1"
                     /translation="MRTKLTSSIMLILMGIAIMVNVGITQETSVIKLQPPQLDRGKPL
                     MQALNERQSNRQFSAKPLDLQTLSNLLWAADGVNRPESGKRTAPSAMNQQEIDIYVVM
                     KEGIYRYDPKEHALHLIAKGDLRNLCGRQEFVAQAPVNLIYVADYRKMARLNDEERKF
                     FSAADAGHISQNVYLFCASEGLACVVRGMVDRDALAKAMQLSPEQHIILAHTLGYPPD
                     "
BASE COUNT         7268 a         5469 c         5616 g         7198 t
ORIGIN      
        1 ttttgatttt ggctattgta atctattctg aagaaagggt gaaattattt tttacaggtc
       61 ttgcttggat cgtcaagttt caggcaagtg agaagattac gaatgacttt ttccggtaat
      121 tcctcgcatt ggtcggcaag gcggtaaatt tcaatcgttc ttttaatttt gtcaatgtaa
      181 acttcacaat tcgggcaggc ttccatatga tcacggataa ttttacaggg agcggagttt
      241 agatcactac ccatatactc gcagatttcc ttgagaagtt cttcgtctag cttcataact
      301 gcatttcggt ttttaacatt ttttccaaat tttctttcag gaaattacga gcccgcatca
      361 gccgaatttt ggtgttggaa ggtgtgatct gcaaaatgcg gctggtgtct tcagttgata
      421 atccatgtaa atcacgcaga atgaaaactg tgcgatactt ttcgggtaat tgttcaatca
      481 aacgatccaa cctttggcga aagtgaggat cattgacgat atcataaccg aaatcaggcc
      541 attcgctggc ggcttcggaa cttactcgtt caaaatcggg atcgtcaagc gaaatgtgtt
      601 catatttgcg tggtttggac tttaactttc gcaagccaac attcatcgcg atgcgatata
      661 gccaagtata gaaagatgat ttgccctgaa attggttgat ctttttcacc ataattagaa
      721 acgtatcttg taaaatgtcc tcagcatcct gatgactacc agttaaacgc aacccgagat
      781 gataaatgcg actgctgtaa ttgagaacca actgagacag agccgcttgg tcgccctttt
      841 tggcgcgagc aactaattct tcttccggta gattgttttc caacgatggt aaatttaatg
      901 aataacatcg ttaattccat tggtatttta attgcgctaa tcaagttgtc ggctggctgg
      961 ataaaatttt ttccagaaat tgtttttctg agacggcacc gtcaatgatt atattttcat
     1021 ttataacgga gcgcggtaca ccgcgaacgt tatacttttg ggcaagatgg ggaaattcga
     1081 tggcttcgat catatcagcc cgaatgtttt cattcaaaag cgcgaaccga tgagcagtgc
     1141 gcaccgccgc cggacaatat gggcaagtcg gggtgctaaa aacctgaata tgcactggcc
     1201 aggtaacttt ttttaggcct gctaagattt ccggagatag gtcggtttta ttattagaaa
     1261 tatcgacaat atcttccagc agggcggcaa actcgtatcc ggctggaatt ccataaaatt
     1321 taatccgaga atggggagca gtttcagcaa acaaaatggt agccggaatc ttgtccacgc
     1381 catatcgttc cgccacagaa cggtcaattt ggaaattgta aacttccaga ctcaatttat
     1441 cagaaagtgc cgtgatttct tccagtaatt cgcgagtctc gcggcagtat tgacattcga
     1501 gagtttgggt aaaatttatg attttgaccg gattattcag gcgactgaaa acttcgcgnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac cactggaacc gatacagccg
     1681 cgcggattat ttgggctaag ctgaccattt tttcactgcc gatcgagcaa ttggcaccga
     1741 ctgccgtagc gccggcttcg actagctggc gcatactttc ttccaccgga ttgcccataa
     1801 tagttacgaa accggtcggg ctttcgctaa aagtcatagt tgcaaaaatc ggtttggcag
     1861 acacttgcag aatgccttta attgccgcca gagcttcatt caggtcaaac attgtttcaa
     1921 ttatgaataa atcaaccccg gctttatcga gaaaaccagc ctgctcggca taattaatgg
     1981 tggcttcttc aatactgatt tttccaaaag gggctaacat ttcaccggtt ggcccaatgt
     2041 taccggcgat taaagtattc ggctttgcgg cggcgcgcgc catttctacc atgcgatggt
     2101 taatctcggc gacgcgtttc tcgacacctg ccttttgtaa ttttaacgat gatgccccaa
     2161 aagtattggt tgtgagaacg tctgccccag ccgcgacgta ttcggcgtga attttggtta
     2221 ccacttccgg atgctccagg caccagacct cggcgttcgc gttcgtaggt agaccatgct
     2281 gaatcagcat ggagccgaaa gctccatcaa acaataatcc cttttttcta gccaatgcta
     2341 ctatatccat ttatctttct ccaattgtga agtaatgtaa aaagccgcgc gatagaaagc
     2401 aaaagataca aaagcaaaat catagcattc ccaacattaa caggattgca cctaattttt
     2461 tgggaatgat aaaagttgct cgttattcta aacttattac tggacttatt cagcggtaaa
     2521 caagagttcg aaaacaagct tttgctaatc ttgtttatct ctaaaaggcg agggtaatgt
     2581 cctgtttttt ttgaatatct attttttggt gatctccata aaattgtgaa ttttgtctaa
     2641 atacctaaat aattttataa atttgaattt aacagtagag taagatggag atttcaattt
     2701 gactaagatt atacgttgcc cctggccagc taatgatgag ctgatgattc attatcacga
     2761 taccgaatgg ggggtaccgc ttcataatga ccaaaaatta tttgaattca tagtattgga
     2821 tagttttcag gctgggctaa gctggcgtac gattctgcac aagcgcgaaa atttccgccg
     2881 agctctggca ggcttcgagg taaatcaggt tgctaatttc ggtgaaatag aaatcaattc
     2941 gcttctggca aatgcgggaa ttattcgtaa tcgggctaaa atccaggcga caataaataa
     3001 tgccgcgcgc tttttagaag ttcaacagga gttcgggtca ttcgatcgtt atatctggca
     3061 gtttaccgct gggaaaacga ttgtaaacca ctggcaaaaa ttatcggaat taccggacaa
     3121 aagtttagaa gccgaagcta tgagcgctga tctgattcgg cgtggctttc ggttcgttgg
     3181 tccgaaaatt tgttatgcat ttatgcaagc ggcggggatg gtcaacgatc atctcgagag
     3241 ttgttttcgt tggcaggagt tacagggcta aagaatccaa tagtgaaata actagagccg
     3301 ctataactat ttgttaattt agtaattgta aaattatatg ctttaatgat aaaatttcgt
     3361 tcaatttaga agttttctac tccgaaacaa gcatctgagt aatttttcaa ttggaaaaca
     3421 agtttattat cttgaaagtg aatttattat ttagattttt gtttgtcaaa gcttgacatt
     3481 aacttaattt atgcataaca ttttccgctt aattaaaagt gcttagagaa cgtaaatgga
     3541 cattttgatt gctactcata atcgggataa agtgcgcgaa atcaagcgaa ttttttctct
     3601 accggagctg atatggcaca ctctggatga atatcccgac tgtcccaaag ttgttgaaga
     3661 cgggaacacg cttttcgaga atgcatttaa gaaagcacgt atgatcgctg aatttaccgg
     3721 attaccgacg attgccgatg acaccggctt ggaagtggat gcgttgcacg gacagcctgg
     3781 ggttttttcg gcgcgttttg cgggagagaa tgctacgtat gctcaaaatg tagccaaatt
     3841 gctggaagtt atgcgggata tacctgactc cgagcgacaa gcgcatttcc gctgtgtagc
     3901 ggtttactac agtccggatt tgactttagt ggaagaaggg aaaattgacg ggatcatatt
     3961 acgtcaaccg cgcggaaatg gcgggttcgg ttacgatccg gtattttatc taccacaact
     4021 cggtcgaacc tttgcggaaa tggacttaag cgaaaagaac tcccttagtc atcgcggtca
     4081 ggcgttcact cgattagcca ataaattaaa atccgttgat aaatttgtaa ccgacaatat
     4141 ttgaggtact ttttttagaa ttgctaccct gcgcgcaata agctttgcgt cggtttaacc
     4201 accataacca ccatattaaa aggttagtaa attaatatca atctgataaa aagatatact
     4261 ttacccatat ttattttaat ggggttcttt gtccaaagtt tggccgcgga cagagagccg
     4321 gacgtatttc ctttaaaaat tgactggctg tctaatcgct atacgccagc taatatcgaa
     4381 agcaaacgcg gtttgtcgtt gatttcaccc gattatgcga ctttgaacca aaatatcctc
     4441 aaagtcaaat ttcccgggat gaaggtgact aataccctcg ataaatccgg ccagaacttt
     4501 attttttaca atgatcttaa cggtgctcca gtccttactc ccattggaat tccagttgct
     4561 agctattttg atctcagaca gcgcctgaat tggcataata aattagtcga agttgttacc
     4621 gccaaaccgg ctgacactaa agccgccagt gataaactgg aactaatcgg tgccgatatt
     4681 gccggtcagc acgtggcttt gcgcgtttcc ggtaatatta atattaacgg acgtctgcaa
     4741 acccagaaac gttcacaggt gagcagtagt tatgcggaag gaacttcgac gagttttatc
     4801 gttgatcaga aacagcaatt gaacattgag ggtaagattg gcgaccgaat tagcatattg
     4861 gttgaccagg atagtgagcg cgactttgat tttgaaaatg ctatgaagat tatttatacc
     4921 ggcaaagagg atgaaattgt tcaaaaagtg gaagccggta acgtcgctct gtccttgccc
     4981 ggcactcaat tcgtaacttt ttcgggcaaa aacaatggct tgtttggcct taaagctttg
     5041 atgaaatttg gcggagttga tgttaccgca atcgcgagcg tcgagaaagg caaaaaagaa
     5101 aaactgagta tagatggtgg ggcgcagacc tcgaacatta gcatcaaaga atacgaatat
     5161 cgccgaaatc tttatttttt cctcgacact ctctttcgcg ataatatgta taggggttat
     5221 cagttcgatc aattatttca cgctgatcca agagggaaag taacggattt gcaggtctat
     5281 aaatctcaga cgatcgaaac tgccggttgt gcctatggta ccgcctatgt tgatccccgc
     5341 gatactactc tttataaaga ttatagcgaa aaccgcattt ttaaattact ggaattgaat
     5401 aaggattatt atgtcgatgt gactttggga tatattcgca tgacaactca agtacaggat
     5461 ggtgaaatac tggcagttgc ctaccgcttg actgacgata ataacgtaac tgtcaccggc
     5521 gaagaatatg gagattataa tcgctctgat acttctaccg ctcatataat tttgaaattg
     5581 ataaaacctc aggaaatgct tcccactcat ccctgctgga atctggaatt caaaaatatc
     5641 tattatttgg gtacaaccgg cataaatagt gacggatttg aactggatat cgtttatact
     5701 ttgggtgagg gtgagcaaaa ttacgattta gtgaccggta agaaattcct ggagatgttc
     5761 ggactagatc gggttgacaa taacggtaat cccactcccg atggtgttat tgatatgaat
     5821 aatggagcga tcctaaactt gtattccggt gaattatggt ttccctattt acgcccattt
     5881 cagtacgact cgacagataa cgccggcaaa cgtaatccta atattagcaa aatatataac
     5941 tgttcggcaa tgtacgatag caaccgaacc gataatgaca ttttcagcga aactaaattc
     6001 aagattatgg taagctataa aaatcgcagt tcgattctca atctaggagc tatggttatc
     6061 gaaaatagtg aaacagtaac gttgaataat agagatttgg ttcgaggtgt agattacata
     6121 atagattatt tttcagggac tttgactctt ctaacatcgg aggcgactaa tccagatgcg
     6181 aaattagaag taaaatatga aaagaatcag tttttccaat tggacaaaaa aacgatcctt
     6241 ggtgccagag cacaatacga ttttggtgaa aacagctaca tcggtgccac tgctctttat
     6301 ttcgggcaat cagtgagtga tgaaaaagtc gaagtcggtt acgaaccgat gcacaatttt
     6361 gtatgggata tcaatggacg ctataatcgt aatctggatt ttctaacgcg agcggttaac
     6421 tggttgccat tagtcagcac caatgcggct tcatcctttg atatcgaggg cgaggttgca
     6481 caggtcttgc ctaacccaaa tactctcagt aacgaagaaa ctggtgatcg ccacggcgtc
     6541 ggttttatag atgactttga aagttccaaa cgagtgacgt ctccgccgat tatgcgccgt
     6601 tattggtcaa acgcggcttg tccccttggc aaagatgaga atgaacgcgg tttcactttt
     6661 tggtttaatc cttatggagg cgtcccaacc aaaagtatct ggcctgataa agaagtaagt
     6721 acaaccgccc aaaataatat tactgaaatt atggtaatga gtgttgaccc gaattggtca
     6781 atagcggtag tggatggagt atctcccgtt caatctgcct ggggcggcat tacctacgcc
     6841 tttccccaga gttattatga tcaatctacg accaaatatg ttgacatttg ggtaaaaggc
     6901 aattccggca cacttcatat tgatttaggt caaatcagtg aagatcaagt ccccaataaa
     6961 aaacttgata ccgaagatat cccggatgcc ggattgacaa tgggcaacgg attactagac
     7021 ccaggggaag acactggtat tgacgggctg gttgataaaa acgaaaaaat tgtgaccacg
     7081 aagtacggtg aactctccta tggcgatacc aggctaaccg aatacaaacg tgatatatct
     7141 gatcctcatt ctgataactg gaaatatacg gaaggttcca aagaatatcg ctatatcaac
     7201 ggcaccgaaa acaatgaaaa acaaaccgac ggcctttttc ccaattcgga agacctgaac
     7261 aacaattatg ccatagacct gacgaatgat tattttaccg ccgcttttga tctgtccgac
     7321 cctaataatg agtatattgc cggaaagact tatatagatt ccagacccac cggctggaaa
     7381 ctctatcaaa ttccgctgac cgccttttcc aaaagcgcta cgggaaaaga tattagctgg
     7441 gaaagcatcc gagcctgtcg tatctggttt gatggcttaa ccggcgaaga ttctgtattc
     7501 atagctaaag tagaattggt gggtaatgaa tgggaagacc tcggaattgc taaagacgat
     7561 acgtccatct tcgttgtcaa tgaaaattcc ttttcgattt cagtcattaa cactgaagat
     7621 aatcctgaca tttatgagcc accagccggt gttcagggtg aatatgatga aatcaaccag
     7681 attcgtacca aagaacaagc tctggttttg aatttgcctg aagagggttc tggtttattc
     7741 cccggcgaat tttgcgccgc caaaaaagtg cttgccgaag aaactagcta catcacttat
     7801 aaagtaatga aattgttcgt aaacgggcat gaactaaaag ccgatggtta tcaattctca
     7861 gaaggcgaag aaacccctct tcaattcttt atccggtttg gtaaaatatt cgaaaattcg
     7921 ccgacgacaa cgaactatta tgaatatcgt caaccgatct atcccggctg ggctgaggaa
     7981 aatgagatgg aactggatct cgattttcta acctcactga aaaattacga gtcggcagat
     8041 gattatatcg gtgatggtga atatcggata tatactaaca cttcgggcgt tacaattcgc
     8101 cattataaag aaaaatttaa cgatgactat actggtcgag aaataataat ttatggcaaa
     8161 ccctcgctgg caaacgttaa acaccttaat atcggtttgc gcaaccgggc tgataatagc
     8221 ggatatgtca ataaatttaa aaccggaact atcaaaggtc aggtctggct ggatgagttg
     8281 cgcctatcgg aggttcgcaa ggacaagggc atagcctacc gcgccaaagc cagcctgcgc
     8341 gtagctgatt tagccagctt tgacgtttcg gttaactata gagacgcgga ttttcatacg
     8401 gttgaacagc gtcccagcct ccagacggaa aatttgaaaa ccaccgaagc cttgacagcc
     8461 accggacgta tttctctgga caaattcacg ccggctagct ggggcttacg cctcccggtt
     8521 agcggctcat atacccaaac caccggaacg cgcaaatata tctatggcag tgatattctg
     8581 atgaaagaaa atgctcccga tagcctgctg gacatctcgc atagttacgg agtaaacacc
     8641 tcgattgaaa agcgggcttc ggacttctgg ctaactaaat ataccataga ccagataaaa
     8701 atcagtgcta acgctacctg gaccgatgcc agctcggtaa ccgtccgcga atcgcaaagt
     8761 gagtcgtaca aagccagcat ttcctataat tttccctttc caaaagataa atatataaat
     8821 attttcaagt ggacagctcc ggtgccgctt atcggaaata aactcagcga aattcgcttt
     8881 tattatttcc cgaacgcctt gacagtcacg gcggatgccg gccgaagtga atcatatagt
     8941 actcctcgct atggcacaac tataacttcc agcaatagct ttggactcaa ccgcaattta
     9001 tcgttaggtt ataaaatatt tgataacctt agcgccaact atacattgaa tcttgaaaat
     9061 gacctgatcg aattatggca gaatcaatgg gaaattctaa aatctgccaa tccgggacag
     9121 attactaata tgagagagtc ttattcggtc aatttcaatc ccaaaatcgc caaatggttc
     9181 aatcctacct ttaactatac gtctaatttt aactggaatg agcctattgt cagtaattcc
     9241 gcaagtgttg atgccatctc taatcagaac cgtttgacta gtacctttag cctcgatcca
     9301 tcggcaatat tttcatcatt gtttaaacct agtggtactg ccccggctcc atctccaact
     9361 cctactcctc ccggtcaaac taccggtggg cgtcgagcaa cgcaaactgg tgccctgaat
     9421 cagcccaaaa cagccgaacc ggcgccgatt acaggcgact caacccggac tgatcaaaaa
     9481 tccggtgaaa aacgaccgcg aaaaccagga tcgaccattg ataaaggaga agatttaaaa
     9541 cctacttcag gacagacact ggaacaaatg cctaagccag ttgacgatgt caagaaacca
     9601 gaagtaaata taacccagat cgtcgccaag tatctgaaaa agatccagcc ttttaatttc
     9661 aatctttcgt taaataattc agttacgaat cggtatcgaa cgggtacacc cgattggcaa
     9721 taccgagtgg gtttaacgcg cgatcctggg ataccatttg tgatggaaga gtatattcct
     9781 accagtgcgg aaaataccag caacagcttc gatttctcgg ttcgctctgg tataaatttg
     9841 ttctcaaacg tagttattcc gctgagtttt gcgcaaaatg tctcgaatac ccagtctcaa
     9901 agtacggtaa gtcgcacgtt gacccgtgac tatattccgt ccggcatcaa tggaaaggat
     9961 ggcttcccat ttcccagctg gtcgttgacc tggagtcaac tggaaaaata ccaaattttt
    10021 tccaagtact ttaagaaact ttctctcgac cacggctttt ctggcaagga gtctatcatt
    10081 actcaaaatg gcaaagagat gagctcgtct tataaaaggt actttcagcc gctgattggg
    10141 ctttctatgc aatttgtgaa tgacatcagt gccagtgccc gggtcaccag cggtcaatct
    10201 atcaataatg cgtccggtgg tactactatc atagatgatg ctggcgcttc catcagcctg
    10261 agctggcaaa aaaaaggcgg cttatccatt cctttaccct ttatgaagga taaacagctt
    10321 aaaaacaaca tcactttcac agccaatttc gattatacca actcgacaac tcgtgccaaa
    10381 cagggctccg ccactaaatt tgccgtcact gccaaaagta atagttggaa aactactcca
    10441 cggatcaatt atacttttac cgacaaagtt actggcggtc tatttttcga gtatggcgaa
    10501 agttacaaca ggttaaccgg caagcgcgtc acccgcaatt acggtttcga cgtcaatata
    10561 gcaattcgag gttaatagtg aaaaaaagtt tttatttaat attaattttg atttcagcat
    10621 gctcatccgg cggtggcaaa tttgatggtg gaagagcctt tcagttttta aatcagcagt
    10681 gtgatttagg cccgcgaaat cccggatcgc ccgggcattc nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn gcggagattc tatctcgaca gcaaccgccc cgcactattt
    10861 atctggtgtt ttttgacggt gaagattacg gatttcctgg tacactggat tattactgcc
    10921 tcggctcaga atattttgcc aaaaacttgc cgattcccaa acccgaagaa gctattatca
    10981 ttgatatgat tggcgacgcc gatttgcgga tcccagtcga gcgaaattcg gtcaagagcc
    11041 atccccaatt aatcaaaaaa ctctgggcta ttgccgatcg gcataatttc agtgccttcg
    11101 ttcatcaaat tgatgaggaa atttacgatg accacctaat gttgattcaa cacgccggga
    11161 ttccgtcggt tgatttaatt gattttcagt atcctaaccg gtttatcaat tactggcaca
    11221 cgacgctcga cacacccgat aagtgtagtg cggaaagttt ggaaatcgtc ggcacagtcc
    11281 tttacgatta cattttttac ggttctgaag aaaggtagcg aagatgattg cgacctctca
    11341 acgctggctt aaagtgctta tccccaaaag cgccggaaat gaagaactca taattgcccg
    11401 gctgatccaa tggggtatgc tcggacttca ggaaaccgat acggactatg aaatctattt
    11461 tcccctaaat atgaaattgt cgttgccgga gctcattgcg gctgaatggc ctcaacttaa
    11521 tatcaccatc actgaaatcg ttgacaatgg ttggagcacc agatggcagg aatatttcaa
    11581 accgcagaca atcagtccac gcattgctgt acgtccttac tgggaaaaac agcttaaaga
    11641 cgtgtcggta gaaattgtac taaagcccgg aatggcattt ggcaccggaa ctcatccaac
    11701 aacgcactta gccctattaa tgctggaaaa atacttgaca ccgggcgacg tgctattgga
    11761 tgccggttgc ggtagcggca ttttaacaat tgcggcattg aagctgggag cggctcaggt
    11821 tagcgcttgg gacattgact cgctggtcat tgataatttc aatgaaaatt tggagctgaa
    11881 tgggctctct cgaaaggtcg cgattcagat cggcgatgta accgccctgc caaattataa
    11941 ctacgatctg attgtcagta atatcgaacg ccaatccaat ttgcgattat tggaggcgct
    12001 tgtgcgccag caaaatcgtc cgctggtgat ttttaccggc ctgcttaaag aagaaaatga
    12061 gatttttact aataaggtct taaattacgg ccgtgaaatt cttgatcaag atactgaaga
    12121 tgaatggctg gcattggtga ttaagtaaat gaaaagaata ataattgccg ccctgtttat
    12181 tacgatttta acgctttgga cttgcgaaaa tcaatcaatt gtggaaaccg ttgagcccac
    12241 gccgattatc gaaaatctga ctttacatta ttcggaattg aatcatagcc tgtttattgc
    12301 cgccaaagtc gaggacgctc aaggttggga taatctcgat tcggtagttt tcaatctata
    12361 tcgaatcaat acgataggct cgatgaacgg tttccttttt aaaagcggaa aattgtcaga
    12421 tgacggtccg ccgcgcgata ttattacgca agacggcgtt ttttcctatt taatagattc
    12481 gaccgaattg ggaagttatg aaggatatta ccgggtcttc gttcaggctt atgatgctga
    12541 cggtaatgtc agcgattcag ccaccgcaac tgaactcgtg caagccaata gtcctccgga
    12601 aatctttctg ttgtcagcac cgacgacttt tgaaaaatgg gacaccttga aattttattt
    12661 cagtgtaacc gataaacagg gctatgatga tatcgtaggt gtttactact cgatcctgcg
    12721 tcccgacggg caattttttg atccgtactt ttatttaaat gatgcaggag aatatggcga
    12781 tgaaaaagcc agggatggca ttttcacggt ttttcagcct tctaatcctg aaagtgatct
    12841 ccaaggtcta tatacgttct atttcttcgc tgaagattat tccggggcga tgagcgatac
    12901 tataacgtta aaaatcccca atccgggaat tcatattaca gccccagcca gtgctttgac
    12961 cctgcgcttc ggacaaactc taagaattat gtgggagtat gcctatatta gtcaggtaaa
    13021 aatcgagtac acttttaatg ccaatgagac caatccagac tggattgtcg ccgatacaac
    13081 cgcggccgct ttgaagtatt atgactggac tgtaccggct actcaggtta ccaactactg
    13141 taaagtgcgc gtttccgacc ttaccaaacc ggcgcgttat gatgtcaatg atgattatct
    13201 ggtgattacc caatgaaaaa atatccggcg attttattca taataggatt tctcttcacc
    13261 tccggtttta gtcaggcttt tcgtctggct aaaccaaccc tggatagtga caacaccttt
    13321 ttgggtctgg caagtaatag tgtaacagaa attgtcagtc agggcaattc cttgatgtgg
    13381 tttgcgacgg gcggcggttt gagtatgagc ccggatttcg gcaagacgtt cgtaagtttt
    13441 tatcccgggt tgaataattt gccacgcggc ggcatttccg caatagcggt caagggcgat
    13501 acgatctggg tggcggccgt ttttgattcg ctgatccaat cttacggtta ccaacaaacc
    13561 ggcggcggtc tggcattttc tattgataaa gggaaaaatt ggactttcgt cccgcaacca
    13621 attgacgatc cagccgataa tagtgctgaa tggaataatc agagtgtttc ctttttgccg
    13681 gtaacgacac cgatcaataa taccacttgg gatattgcta taactgatga ttacgtctat
    13741 atcgttagct gggctggtgg tattcggcgg agcgctgata tgggacagac ctggcaaaga
    13801 attcctttac cgtctgatga ccttaataat ttggactgtg atgatctgat agacttcgag
    13861 attaatccgc gcgatccacc ggatggcaat cataatcata aaggttttag catactggct
    13921 tatggcgata cgatctggat cgggacagct gatggaatta atctcggcat agttgaatct
    13981 ccagagtgca ttagctggcg caaatttaac gcccagaatt ccgcaatttc cggcaatttc
    14041 gttgtagcaa ttgaccgcca actttggaaa ggcaaagaga ccatctgggc cgccacgttg
    14101 acagtcggag aaagcggaga atatcgggct gttagcaaga ccagcgacgg tgggttgact
    14161 tggacgacaa ccttagtcaa tgaacgccct tataattttg cctttcagga ttcaatcgtc
    14221 tatgtctgta cggagagagg cttattcaag tcaattgatg gtgaaaattg ggctatttat
    14281 acaccgattg ccgacattgc ttccggtgaa caaattttaa cccaatattt ttacgcggca
    14341 gaagttgatc gacgcgaagg tccaacctat ttatgggtcg gcaccggaga cggcgtcgcc
    14401 aaaaccgcta gcgatggatt gacctggaca gtttatcatt cggctgtttc gaccaaaagc
    14461 cccggtcaac cagccattta tgcctatccc aatccgtttg cgccgcgtta tcaaaatgta
    14521 ataggtgatg atggccatgt tcgcattcaa tataatatag atcagcccgc atcggttcga
    14581 ctggaaatct ataattttgc gatggaacaa gtttaccgca gtgattggaa aacggttatg
    14641 gatgccggag atcatcattt gatctggaat ggtcgggcaa cgaatggcga gattgtagcc
    14701 aacggcactt atttctgcaa actgatccaa aaatcaaatg gaaatgaagt ggagcactgg
    14761 acaaaactga tcgtgatcaa ttaattatga gaaagtttct gaatcttata ttatctctta
    14821 ttttttcgat ggctctattg cagggcgaaa cgaatgacaa tgctggttat gccggggcat
    14881 ttctgcgaat gggtctcgga gctcgcgcaa cagcgatggg taattcggga gttgcctttc
    14941 catctaacgg ttttggaatt tactataatc cagctggttt ggcatatatg gatgaaagaa
    15001 acttggcgct ttcctattct tttttgagtc ttgatcgcca gtttcatttt atcggattat
    15061 cggttccgct tccgccaaat gccggtgtgg gagtatcctg gcttcatgct ggagtaaagg
    15121 acatcgaagg tcgcaattcc attggtcagg ttgatgaaac ttaccaaacc ggcgagaacg
    15181 tggtcttgat ttcctttgcc aacgcttttc atccccgaat ctctctcggt attaatttta
    15241 aaattttaca taatcaaatg ctcgacgtca gcgctaccgg actcggattc gattttggtg
    15301 ttctttatca gccattggaa tggcttacgg ctggcttgca attcaaagac atcggtgccg
    15361 gttataactg gaatacacag gatttatttg gtgaagaagg cagtagttat caagaaaatt
    15421 ttccccagat actcaaattt ggtctggcta ttaaacccat acaggagctg accttcgtcg
    15481 gagatattgg agtctccgat cagaatgtct ggcaaactca ttgcggtgct gaatatgttt
    15541 atcgcaacct ggcatttttg cggatcggga tgaaccaact aagtccgact tttggagcgg
    15601 gtctggcata tggttttatt gctaatatga ctactcaatt agattattct tttataatag
    15661 gtgccgttgg tgaaggcacg actcacattt tttcatggca atttcaattt tgatggttac
    15721 tatgcgacaa aaattaagaa caggatttat cataattatc accggcatgt ttttacaggc
    15781 cgaaagcggc gaatcaggaa tggcgttttt aaaaatccca gtttcagctc aaactgcttc
    15841 gacgatgggc gtttttgccc aagtcagcgg cacgccgttt accatttttg ataatccgat
    15901 tggaattata aatgacgaca ccaagatcgg cttcagccat aatttctggt ttgccgatgt
    15961 cagcggcgac gctctggcgg ccacgcttcc tctcaaaaaa ggcaatctcg gattcgggct
    16021 taatttcgtc aagattccag gtatcgaaat tcgagacatt ccttctgacg aatcgttagg
    16081 tgaggtcgaa gcgcaatata tgtcagccgc cgtcggatat gcttatccaa ttttttcgaa
    16141 attgtctatt ggttgtaccg tgaaatatct ctacgagcac ctctacgccg agaatgccgg
    16201 tgggatagca tttgacgttg ctactcactg gcacattccg agcgggatgg atttagcatt
    16261 atcgctaaat aacattggcc agatgcaaac gctgaaagat aaggctacga ctttgccgac
    16321 tcttttcaaa ataggaatcg taaggccgga aattttcgcc gacagccctt ttaatgttaa
    16381 tctcgggata aatttaatcg ctaatttggt aaccgaaaaa acctactttc aggtcggcgg
    16441 agaggtggcg ttcacgaact taatcaaaat tcgaggcggc tacgaacggg tcgggacaat
    16501 caaccggact gccctggggg ccggtctaaa tttcgggcgg ttcgaaattg attatgcctt
    16561 tatttttatg ccggatgggc tgggttatcc caatctcctt actcttagct accgaccaga
    16621 actataagct tactattctc gaaataagcc aaacagctat caaaatatat cagcttacta
    16681 acccggactc cactttggtt ttttgactat ttcttttcga ggtaagcatt catcttgtag
    16741 tgagttcact cgttaaaata tcctcagaac tgaccggatg tattaaacac tttttatcag
    16801 catggaaatt caatagattt taattatgtc tgatagaagt ttattactta ttaatccctg
    16861 gatcgccgat tttaccgctt ttgatttgtg gtcaaagccg ctgggcttgc tgtatattgc
    16921 caaatttcta caaaactacg gttatgaaat cgaattactc gatttaatgg accgtcaacg
    16981 ctggcaagac accagtaatg ataagttcaa tgacggacgg ggcaaatatc gtaaaaccat
    17041 cattgctaaa ccaccggtgc tggcgcgcta cccgcgacgt tttggccttt atggcgccag
    17101 ccgggaacag ttcagtgacg ctctctccaa tatggcgcta ccgaacgcca tcctagtaac
    17161 gtcgttgatt gcctactggt atcccggggt cgccgccacg gtcgaaatcc tgcgccgcta
    17221 tttccctgaa acgcctttga ttcttggtgg aatttatgct agtttatatc cagagcatgc
    17281 ccgaaattca atccagcccg acttcttgat aaccggttat ggtgaaaaga aaactctgca
    17341 gttggttgac cagctttttg gaattgaacg ggattataac gttataccag attacaatga
    17401 taacggcatc ttgccctggc gtttatatca aaagctaaag tcagtggcag ttctgacttc
    17461 acgcggttgc cctaatcgct gtacttactg tgcgacttac cttttaaata aaaagtttgt
    17521 tcagcgccgt ccggaggatg ttattagcga aattatttcg acttacaacc agtttaacgt
    17581 agagaatttc gctttttacg atgacgctct tttcgcccat cgcgatcgcc acattattcc
    17641 aatattaaag gggataattt ctgccggagt aaaaattaac ctgcattcac caaatggcct
    17701 ttttgcccgt gagatcaatt ctgaattagc cgaactaatg ttctctgccg gtttcaaaac
    17761 tgtgcgctta agtctggaat ctacttcgtc tcaaatccaa aaatcctcca gcaataaagt
    17821 caataatgcg aattatacgg acgctgtcaa taacctggct aaagccggat ttgcgcgcaa
    17881 ttcactggaa acttacttga ttatgggctt acccggtcaa actcgccagc agattattga
    17941 tagtattaat ttcgtcgccg atcagggtgt gattgtaaaa ttggcatctt tttcgccgat
    18001 tcccggcaca accgaatggg agcgtgctcg tgcgcttggc tatgtcagcg atgatcaaga
    18061 tccgctgatg accgacgcca cgatttttcc gttcctttcc cgcaatctca gctatgaaga
    18121 ttacttagct ctgcgccaag tagccaatga acgcaatgcc gaaaatcgaa gcaataacca
    18181 aatttctaat gaggcaagtg atgggacaga ttaaatccgc accgcaaggt catttatttt
    18241 tagcaataat gtctgcgagt gagcatcaac gccaagtcgc actggaggag gtgcggactc
    18301 gttttggtga aataatgggc gccggtccgc ttttttccgt cagcgacttc accaattatt
    18361 atgaatcgga attcggaagg gatttaaaga agcaattttt cattttcaaa caacctacca
    18421 gcctggaaaa tgctcaccaa attaaagtct ggaccaatca aatcgaatcc caatttgtag
    18481 ataataccgg cagtcaatcc catcggcaag ttaatcttga cccgggctac ctcgaaccgt
    18541 cgaaattagt tttattttct actaaaaatt tctcgcatcg aatctattgc ggtgacggtg
    18601 tcttcgcgga ggtaacgcta atttacgaac atggccaatt caaaattcta ccttggacct
    18661 accccgatta ttattggacg aaaaacctcg aatttctaat acaaatgcga aatgaaattg
    18721 tgcgtctgag tcggaaggat agtaaacagg cttgacagta tttttataca agattctgct
    18781 atcgatcaac aaagaaatcc aattaaatct ccaggatttc tccaatgata actacttctt
    18841 tcattgaaaa aatttcctta aatattttgt caggctaatt ctgctaaaaa aacctaaatc
    18901 cggtcagtcc cgtctaaaac catgctacta cccgggcggg ggcgccatcg gtagcaatct
    18961 tgagcggcag agcaatcaag tgaaaaagtt cctgcgttgg tagcgcaccg agattggtca
    19021 gattctcgat aattaatact tcatgttcca ttaagagatg gtgaattgga aaattttgcc
    19081 tggcggcatt agcggcatcc atactcggga aatcgccgcc gataccctta atttttagat
    19141 caattaattg ctgggcgagt tcaactgata gatagggatg ttctaaaaaa taactcggct
    19201 gactccaaaa ccggtcaaat ccagtgcgca gaatcagcca ctgcggtttt aagttttgaa
    19261 ttaattcaat ttcttcgtca tttagattca gagcggatgt gccagccgaa tcacgatgta
    19321 agccaagcgc cgcgccggta aagcggtcca cattaaaatc agcgatgctt tttccatctg
    19381 gcaaaaaatg gcgcggagcg tcaatatgcg ttccataatg agtgcctaaa tgaatttctg
    19441 aaatcgtgaa ttgcgtctta ctgcgcgtaa taattcgcgg cttaggacgg tcgcccggaa
    19501 aggtgggcat accgttttcc agggtatggg ttagatcgag gaaagtaaat gctttattca
    19561 atttcttatt cctgacgttt tgaataatgg attaaaaacc aagccacagc ccaatcaaac
    19621 cgactaatag accaaaccca aaattgacag ccttgatgcg aacaaaatca cgccgcgaat
    19681 gcgccagcat cggcaacata ccgtgaccgt cttggacaat cgaactggca agtaaaatac
    19741 taaatggcac cagcccttcg gcgaacattg tcacgaaaat taaatgcggt cccgattcgg
    19801 gaattattcc aaccaaacag gcaactaata aaaccagaac acgtgcctgt tgcaattgca
    19861 ataagttttc gatattcaaa taattggtta aaatctggat tactatcaaa gctccgagtg
    19921 tccagaaaaa aattctgggc acatgccgtt tagtaacgtg ttcccagaga tgttccttca
    19981 gaaagtggtc aggaaccgtc gcaacaatcg ccagcccgac tatcgaagta ataattaacg
    20041 taattcgcac ccagccctct tcctctccgg cgatgaaacc gctcgcggcg gctagcaaaa
    20101 tcgccgccat aattaaaatt aacagaaatc gttcgagagt aatagagcgt aattgttcta
    20161 aaatctttcc ccgctgaaag cactcacagt attcatcatg taacggtaag tcatgagcag
    20221 ttactacgtc atattttttg cgaaataaaa gatcggctaa cacgccaacc gggattgaaa
    20281 tgatggctaa aattacattc aataacaaag ctttgccggg gaagagggca aacattacat
    20341 aagcctcgtc gccgctggtt gcgatcatcg ttgttactac ggcgcctatg gatactaagc
    20401 ggtgagtgta aagcgtgacc atcgcaaatg agccgagaca gcctggaatt atgccaagca
    20461 acgctcccag aacatattgc cgccactttc gtcccttgag taatttgtgc cattttcccc
    20521 gagtctgaac gtttaaatat tcgattacca gcatcatagt gaaaacgaag ccggttatca
    20581 ttatggtaga tttgattacc tcaagcaaat tactctcctt acttttttgg gctggctttt
    20641 aaagtcataa tatgagcacc atttcgcagt agttccgttc gatcagtcaa caaattgact
    20701 ggttctactg gattcgttgt accgttgact gcaatgcaac aagtcgtttc gcttccggca
    20761 ggttttaaga ttatagtttg gaccacgccg gaagagttga gactaacact tttgctattt
    20821 gaaaattttt tcccctttat gttgttgccc catacgaaat cctgatttac tcgtccccat
    20881 tgccattcgg atagattcgg cgagtacttt tcctcgatca actgcactgc ttccctgaat
    20941 gattggcgaa taatttctcc taaccattca acaaaatcag atgttcgtag attatcaaac
    21001 caattgctct cgcctttctc gattagtaac agcaaatttt gcatagcaaa atcaccggaa
    21061 ttggcaaatt gctcaaacgt ttccgaatca actaaattta gttcgtcgct gtaaatattt
    21121 cgcattagcg ctttcagaaa agcctgataa atcgttggac cgacggcggt tttagcatag
    21181 cggctatccc actcaattag attctgatag gcttggagtt ctgtcttgcg cgtaaaaatc
    21241 gtgtcgggta gagcggtgcg tacatctttc atcaatcttg cgaaatattt atcggtagcg
    21301 aggattggaa aaaattcgtc agtggattcg gaaaataacg ctttaggttg gtcttgccaa
    21361 tccggtaaaa aatgactttt cagtgaatct atcgtgaatt gagatgaaaa acgccaatcg
    21421 tcaatcgtga agggcgtcag gcaattaatg ctaagattag gcgtcagcgg tttgggtaaa
    21481 gcggaggaac catagttacc tttttggtca agataaacaa gacccggcag attggaactg
    21541 acttcagtca taactcttgc ggcgtcgtta ttaccaatta acattaatgc tactcgttga
    21601 tttatttctg aggcagtaac tgcctgccat ttcaggacga tagcgtacgc aaccgtatca
    21661 accgactcga ttggataatc gagaattaca ccctgatcag tatgataaaa tgtaacctcc
    21721 tgcttatggt cactgcggac ggcaatctgc gtgctacgct ttcgaagatt gtgccagccg
    21781 tcttgcgcta aatattgttc actagccgga ttgatccgaa ctaccaacaa attgacctca
    21841 ttttcgggca accagttagt cccaaatgct acctgctgat tacagcctgc aaggcaaatc
    21901 gggacgcccg ggaaaaacaa cccgactatt cgccggtcga gaaccgccat atcgaaaatg
    21961 aaatcgtaag agttaaacca attattttca aaaattagtc gagtctggtc agcgggaaca
    22021 tttttaccag ccaatatcca ggcgagcgat ttatctttat tcgggaaaag gttcatagtc
    22081 cggcgcaaat taaccccatc ccgatacagc tgattaaggc tggcaaagaa ggcgttttgg
    22141 taacccgatt cgcggacagt acttggctca atcaggggaa atccgtcgcg agttcgttcc
    22201 tccccgaaca cttcgccggt tttgtaaatc acgatttttt cattccattg gcttaaataa
    22261 gcccatttaa ataactgtaa aatggcgaga cattcggctg gttcccattt gtgcggttca
    22321 taaccggcca ggcgaaattg ttgggcaact gaatggcgct gggtggcgat gaaagcattt
    22381 attcctgcgc aataagcctc aaaaagtgtc tgtgcttcgg gcgaaagttc tgccagtgtc
    22441 tgttcggcta aagcgacaaa atcaagttgg tgggcggcga tgtccaattc gataaaattt
    22501 ttaccatgtg cggcagacat ctggccagtg gcaaggcgca agagacgatc cagatttggc
    22561 aattgtcggg tggcttggag atagccaata gttcgcagaa gatcagcatc attaccggca
    22621 ctaatatggg caatgccgtc cactccggtg agaatttcca cgtcttgaga caaacaatcg
    22681 agaacgatgt caacatttcc aaattctttt ggttgactga agaatccgaa aacgaccgcc
    22741 gcgaccagaa caaccaatcc gataattaaa gtcgcgatat tacccagaaa ttttttagga
    22801 aaatgtttct cgtgattctg tatcattcta cggtaaatat aatttatttt aaggggcgtg
    22861 acaagaagat gtaaaattgc ttttacgctt gaagttgatt aaaataatat tgtaaaaaag
    22921 gagaatgcat tatgagtgag ctgattaaaa aaattttgct ggcaagtgtt ggtgctgttt
    22981 ccttgaccaa ggagaaagct gaaaacctgt tggaagacct ggttaaacgc ggtgaaattg
    23041 ctcgtgaaga caaaaccagc ctgctgaatg aaatcttgaa gtctattgag caaagtcaac
    23101 aggaaactcg caaatttatt caaaaagaaa tggagaaagc cctgaaagct cttgacattc
    23161 cgactcgttc ggaatttgaa gagctcaaaa aacaggttaa atcccgtcag gcgcacaaag
    23221 attaagtccc ctgatgaggc gtttttccgt ttcgcgcact tggcgtcatc tcaaacgcta
    23281 taatcaggtc ctttcggttt taatcaagta cggtttttct gatatagtgg atgccgcccg
    23341 taaagatctg attgcacgtt tcggtgaaaa atttatcccg cgcggtcgca ggaaccaatt
    23401 cagtggcctg tcccatgcgg agcgaatccg tcgagcgtta gaagaactcg gtccgacttt
    23461 tgtcaagatt gggcaggttt taagtctgcg tccagatatt attccggctg aaattgctgc
    23521 cgaacttgct aaacttcagg acgaagtttc accgctccct tacgacgtta ttgctaatgt
    23581 aattcagact gaattaggaa gcgaacccga taccgttttt caggatattc aatcagaacc
    23641 gttagcagcc gcatccctgg ctcaggttca taaaggcgtt ctaattagcg gtgaaccggt
    23701 tgctattaaa gttcagcgtc ctgaggctga gtcgctcatc aatactgata ttgaaatttt
    23761 atacgatttg gcatctatta tacaaaatta ttttctggaa aactttgctc ttgatatttt
    23821 ggctttcctg cacgaatttg atcgttcaat tcaccaggaa ctggattttg tactggaagg
    23881 tcgaaatctg aggcgttttc gcaaattttt tgccggtgat gaggctatct ttattccgaa
    23941 ttacttcgcg gagtatagta cttcaaagct attagtgatg gaactggtag aagggattaa
    24001 ggctacagaa atcgaacaac tggaagcggc tggtatagat cttcaagtaa tcgcccagcg
    24061 cggcgtcgaa gccagttttc gtcaggtatt tgaatttggc tttttccatg ctgatcctca
    24121 tcctggtaat ataatgatcc tgcctggcaa cattatcgca ccgatagact tcggtatggt
    24181 tggtcaaatt gatgaagaca ccatagataa cctcggccat gttgtcgtcg gcgttctgca
    24241 taaagacatt acccgaacct tgcgttcctt agaagacctg actggtgctg atttatcagc
    24301 agtagcccat gatttacggt atgacttgat gacctatatc aataattatt ctgaattgcc
    24361 cttaaaggat ttacagctga gccttctaat ggatgacttt tttgagttaa tacggaaata
    24421 ccatctgcgc attccttcgc atttagcttt gatgttgaaa gctctggtta cagtggaagg
    24481 attggcgcgt attctctggc cgaatttcga cgtattggaa gctttgaaac cttatgtaag
    24541 aaaaattacc ctgcgccgtt ataatccgcg ccggattatg cgaaacggaa ctctcgtatt
    24601 agacgacttt gccgatttga tgaccgatct gcctggcaga ctacaggcaa ttttcaaaaa
    24661 agccgccgaa ggtaactttg ccattcaatt tcaacaccgc aatcttgaac acctaattga
    24721 tgagttccgg aaagccgcca accaactttc gctggcatta attctggcgg cattggtcgt
    24781 aggttcctcg ttagtcattc tgacaatggc aggacccaaa ttattcgggt actcgttaat
    24841 tggcttactc ggttatgtag ccgccttgtt aataggtctg agaatgcttt ggaatcattt
    24901 tagaaaaaaa taaaatagga gtagaacaaa aaaatgagaa cgaaactcac tagctcaatt
    24961 atgctaattt taatggggat agcaattatg gttaatgtag gtattactca agaaacttcc
    25021 gtcatcaaac ttcaaccacc gcaattagat cgcggcaaac cgctaatgca agcactgaat
    25081 gagcgccaga gcaaccgcca attcagtgcg aaaccacttg atcttcagac tttgtcaaat
    25141 ttactctggg cggcagacgg agttaaccga cctgaatccg gcaaacgcac cgcacctagt
    25201 gctatgaatc aacaggaaat cgatatctat gtggttatga aggagggcat ttatcggtac
    25261 gatcctaagg agcatgctct tcatttgata gctaagggtg atctgcgaaa cctttgtggt
    25321 cggcaggaat tcgttgccca agctcctgtc aatttaattt acgtggcgga ctatcgtaaa
    25381 atggctcgac taaatgatga agagcgaaag tttttctctg cggctgatgc cggtcatatt
    25441 agtcagaatg tctatttatt ttgtgcatct gaggggttgg cttgtgttgt ccgtggaatg
    25501 gttgaccggg atgctttggc taaagccatg caattgagcc cggaacagca tattattctg
    25561 gcacatactc taggttatcc tccagattaa atgcgaattt tgaattacta aaaatttccg
    25621 acttttaaaa agcgaaggcg agcagggact catcgtatct gttcgccttc tagtgcccac
    25681 tcggccccca gtatatttag ggtttcctga aaaagtcacg actacgttaa aactggtata
    25741 atttcttgat t
//
LOCUS       DAOOMU010000022        25345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1833_length_25345_cov_24.243970, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000022 DAOOMU010000000
VERSION     DAOOMU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 25345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25345
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1833_length_25345_cov_24.243970"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..1678
                     /locus_tag="PLI55_03460"
     CDS             17..1678
                     /locus_tag="PLI55_03460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPA99928.1"
                     /translation="MLSEDGSIYVHLDWKKGHYVKVLMDEIFSETNFKNEIIVKRGQK
                     NVQAQFTTIDSLASGHDTLLFFSKQSGTRFSILKSSLDEMKPGAWNNHWRGTDRPAMR
                     YELFGILPDRGQWRWSKERTMKAVENYERYVNQYSKTMSIDEYYVHILETTGEKLNFV
                     RLSSTRKPEHYVPPTEHQILSDVWMDVTAYEARKDYPTQKSEELLSRIICGFSNPSDL
                     VLDCFAGSGTTLAVAEKLGRRWIGVDCGKLAIYTMQKRLLNIAESKDLEDSQKKKKYG
                     KPCKPFTLYNAGLYDYKMIKELPWEQYRDFALKLFQCRDERHEIARIELDGYLGADSV
                     MVFNYQKYPDAVLDRGFIDDLHEYLGDRIGKRFFIIAPAASVQFLEDYIEKGNTKYYI
                     LRIPYSIIEEIQNRGFSKIKQPASEMDVNDTVEAVGFDFIQTPMVECKYCLDNRKGQL
                     DFGKGSRDCVIRIEEFESRVISRKPLDFANLETLSMVMLDYDFDGEVFDLDEVFYAEE
                     LKKNNYELRFAEDKVKDKIMIIYIDIFGNEKREIKSLKDFKKGIS"
     gene            1680..2852
                     /locus_tag="PLI55_03465"
     CDS             1680..2852
                     /locus_tag="PLI55_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPA99929.1"
                     /translation="MEINTNTQNYSTLITDLTSLIEQGRQVAVRYVNTALVTTYWLIG
                     RRIVEFEQKGKERAEYGAALLKHLSDDLTKQFGKGWGEPNLRAIRQFYMIYGDIGKRY
                     TVCSESTKSDGTIIRHTSCSELVPSQEIPSELQTLSIETFHNLFPLSWSHYRLLMRLD
                     EPFKREFYEAECIRGNWSVRQLDRQIQSLLYERTALSRRKLAVIAKAHKNPIILKPED
                     EIKDPYILEFLGLKDEYSESQLEEALIRHLEHFLLELGVGFTFVARQKRIDLEGSHYR
                     LDLLLYHRILKCLVAIDLKIGEFGYTDAGQMNLYLNYLKDKEKFPEENDPVGLILCTD
                     KKKTVVEYALGGMSNRIFASKYKLQLPDPEVLEAEITREKQRLLEMKIAGEEIKIK"
     gene            2855..5353
                     /locus_tag="PLI55_03470"
     CDS             2855..5353
                     /locus_tag="PLI55_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPA99930.1"
                     /translation="MLEKQTFLNKDLVLRVSPSYDPRKFEPNKYEAFLDALCGNREYQ
                     KEAIRITLRYFLGGEYQSLRELAEENYHSNPKLQEKYSSFEDFSRVLQLPDQLSGSLD
                     HATATGKSYVMYGIARIMLAEGAVDQVLVLCPSNTIEAGLKEKFLSLSADKTLKDLLP
                     SDSIVANPRIINASNTIQRGDICIENIHATYINTKSAIEDSLVGKGERTLVLNDEAHH
                     LMNPSDTALKKWKEFLLDSKYGFKFIVNVSGTCYIGSEYFTDVIHRFSLKNSIEEKFV
                     KSIRYVAEDSSGSEDEKFQKIYENHIENKTVKYREVKPLTILVTKDIAACKRLTEKLT
                     TFLAEKENISKEQAAEKVLIVTSANEHKNNIPILRRVDDRDNPIEWITSVSMLSEGWD
                     VKNVFQIVPHEERAFNSKLLIAQVLGRGLRIPPVYKGEQPIVTVFNHDKWSSNIKHLV
                     DEVLEIEKRLYSYPVEKKGDYNFDLYNINYKRTEEKVEYPQENPYELLKKEYIAYSSQ
                     AEVVPEDTVYVTAINRAMETVTYSITHKLYSVEEIAQDVFNRLLIFDQEAETEYSQKF
                     TKQKIAKIIRKSLAEIQDESGKVNETNRIATLKAFGVIKRKGTKSLRFKIEAQKLLKI
                     STKEIRKNSLGVGSLRHDSTVFWDDYSITLGESADIKLLKEIEEDESLPRSAIIKVGN
                     KYNFKTPLNVAFASHEPERRFIRGLTSDKVSRTINAWIKSLDVGFYSIDYSWRKGEHP
                     KQGSFNPDFFIKIGQDILVIEIKSDDDISDENRAKLRYAREHFARVNDLQKEQRYYFK
                     FLSPASFELFFQMLSNGTYQDFKSELEAKLENRD"
     gene            5595..7412
                     /locus_tag="PLI55_03475"
     CDS             5595..7412
                     /locus_tag="PLI55_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HPA99931.1"
                     /translation="MFGKGIRRLFFGLSVLLVAMAYGDSNIVINPGFEDGKEGWFDRT
                     CAIEIVTSPVYEGRYSGKAFQRLANWQGIKQSVFGRMVDGKTYQVSGWVRLDNAPSDE
                     VAISFEQQDDSGTKYIGVARGIATDTAWTKLSGEFTLNVTGTLSVLDVYFEGPAPGVN
                     FYVDDVVVYGPEANPPVIIPPTPQSKSQVDAKIRHQIIEGFGASGAYYTMNLVNHNKK
                     TELYNLLFKELGLDIFRIRNNYDMEPHSMQETVEIIKNAKALRGDNLKILMSSWSPPA
                     YLKSNNNVVGGTLKKVAGKFVYEGFADWWYESLMAYANAGVTIDYISIQNELNYEAPW
                     ESCVFAPSESDDTTLAAHNVAYETVWQKLNAEMGKTMPKMLTPETSGLGDSEVYIKNL
                     IDLSHVYGYAHHLYDCSGCGADPDRFIPRMISYREMVKKYGAKPIFQTEFEDDPGAWP
                     DALNTALLLHNSLTVEQVSAYLYWDLFWAPGSGLISLNDFDTYTIKPTYYAFKQYSAF
                     IDANWQRVEVTNDNTGLRISAYVSPDNKKMTIVLLNITQDMDIKLKLAIKNFASAEGA
                     IYRTSETENCVYVGEFNKRKPLVLPANSITTLSLSTAEK"
     gene            7505..9532
                     /locus_tag="PLI55_03480"
     CDS             7505..9532
                     /locus_tag="PLI55_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPA99932.1"
                     /translation="MKSVRHTIANGGLNRQHLQYQTSALVLVILLIMGFLSFVSAEPT
                     KWVGTWSTAPQLVETNNNPPSPGLSNNTLRQIVRVSIGGDTLRLRFSNEFSTKPVTMN
                     SVHVAISTGGGAIDTATDKPLFFNGSPNVTMEAGKAVTSDPFQFTLPPRADIAITIYF
                     GATSPDVTGHPGSRTTSYLLTGNAVTRADCAGAVTTDHWYVINTIDVKAPDSAAAVVI
                     LGNSITDGRGSGTNKQNRWPDELARRFQENPATRFVAVLNQGIGGNCVLSGGLGPTAL
                     SRFERDVLNQSGVRWLIIFEGINDIGNGAPGVGNQLIDAYKQMIVNAHSRGIFVYGAT
                     LLPMKGSFYYSTAREAERQIVNDWIRTSGFFDGVIDLDRALRNPADTLSLLPEADSGD
                     HLHPNEVGHRLIAEAVDLNLFIGREPLDLTDESERIYFEPECAIVGENWDILTDSQAS
                     NGKYVTVKAGMQSLDSAPSGDTNAIHIPFSVDSAGSYYLYARLNCPSYDDDSYWVKMD
                     GGNFQMFNGLVTTGWEWKNLSSYTLTKGEHKLTITYREDGAKLDKICLSNSAYAPIGM
                     GDEAENICSPAGMGLDNSLKVPDRYALRSNYPNPFNPTTTIAYQLSETAFVRLAVYNM
                     NGQLVENLIAKEQSAGYYDTVWNATNYSSGIYLYKFQAGNFQQVGKCLLLK"
     gene            complement(10010..10555)
                     /gene="cobO"
                     /locus_tag="PLI55_03485"
     CDS             complement(10010..10555)
                     /gene="cobO"
                     /locus_tag="PLI55_03485"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPA99933.1"
                     /translation="MSRFQTKRFRMQGLIQVYTGDGKGKTTAALGLALRAAGAGLKVF
                     IGQFLKGAEYSEIKALRQLAPGIEIKQYGRRCFIKDKPDEEDIRLAQNGLVEMESVIQ
                     SGDYDLVILDEANIAVYYNLFSVDELLKVVQNRPKQVEIVITGRNADPRIIAAADLVT
                     EMREIKHYYQKGIAARLGIEM"
     gene            10593..12101
                     /locus_tag="PLI55_03490"
     CDS             10593..12101
                     /locus_tag="PLI55_03490"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPA99934.1"
                     /translation="MKSNAKVQIRLEPLGRVIEVDAGSALKDILFEQGVEFPCGGRGV
                     CRKCRIKILKGNLKVNEIQKKALTSEEISQGWRLACQCQVTEPLVIELAQLEIPILSD
                     VTRIKLVGESGLAVVVDLGTTTLVAQLLDLSSGIVLGVETALNPQARHGADIMSRIQF
                     GLTTAGRELLQNLIRHQIGQMITRLVEKCRCPERRLKQINIVGNSVMHHLFGGLSVEP
                     LALLPFEIEQPGELEWSAEEIGWQLPGNPKVKFLPCLGGFVGSDILAGILAVGMHRSP
                     ETVALIDLGTNGEIVIGNRDRILCASTAAGPAFEGAQITMGMRATTGAIWKVWLTNGQ
                     LQVEVIGNVPARGICGSGLVDAVAAALDLGWIDPTGRITLPKREIPLQKPVVITQSDI
                     RELQLAKGAIAAGIQILRKQMQLKPADIKQVFLAGAFGNYINVVSARRIGLFDFPTEL
                     IEQAGNTALHGAKLSLLNEQEYHSIPLITKHFPLATDAQFMEIFAENMNFPG"
     gene            12486..13832
                     /locus_tag="PLI55_03495"
     CDS             12486..13832
                     /locus_tag="PLI55_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014707689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HPA99935.1"
                     /translation="MLIKIKKGLNLPIAGAPEQVIYPAQSVSQVALLGLDYVGLKPQF
                     AVTIGDQVKLGQPLFSDKTYPQIRFTAPGAGQVIAINRGTKRAFLSLVIKLDGDDAVE
                     FKSFSEQQIGSLTQEEIINQLLESGLWPSFKARPFGKLANPESQPHSIFVTAMDTNPL
                     APDVAVILREQSNDFQRGLALIAKLTAGKVFVCQAPDTAIPVPEIENIEIVEFAGPHP
                     AGLAGTHIHLLDPVSLHKTVWSIGAQDVAAIGKLFTTGRLPTARIVALAGSAVKKPRL
                     ITTRVGANLSELTDGELAEGHNRIISGSVLAGHTAAGELNFLGRFHQIVSVIPEGGRR
                     KLFGWLSPGWRLFSVKNIALAAFLPNRKLVFDTDTHGSERAIVPIGSYEKVMPLDILP
                     TYLLRALAVDDLEEAEKLGCLELVEEDLALCTFVCPSKIDHGQNLRRVLNLIEKEG"
     gene            13832..15034
                     /locus_tag="PLI55_03500"
     CDS             13832..15034
                     /locus_tag="PLI55_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013887005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HPA99936.1"
                     /translation="MGGFRKFLDQAGKPFRPGGKLAGLYYLFEAIDAFFYTPGKVTKG
                     TVHVRDFMDLKRMMITVFVALLPAVFMAFYNTGLQANLVLQSLGSGANFTWQQRLMLA
                     LGLDFNPESFWSNFIHGGLYFLPVYIITLIVGGFWEVLFAVIRKHEVAEGFFVTSLLF
                     PLILPPTIPYWQVALGISFGVVLGKEVFGGVGFNVFNPALVARAFLFFAYPAQITGDK
                     VWIAVDGISRATPLAQFAETSSAVTTSWLDAFLGFIPGSMGETSTLACLIGAIILLVT
                     GVGSWRIMLSILIGMVTLSTTFNLMGSATNPMFAVTPLWHLVLGGFAFGTVFMATDPV
                     SGAVTKPGQWVYGLLIGVMVIMVRVLNPAFPEGMMLAILFGNMFAPLIDRIFINAHIK
                     RRATRDVR"
     gene            15024..15824
                     /locus_tag="PLI55_03505"
     CDS             15024..15824
                     /locus_tag="PLI55_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HPA99937.1"
                     /translation="MSDESARKTFLVALGVCLICAVMVATSVVLLRPRQQVNQELDKL
                     KNILQAANLYTDDAAVKKTFQEKIRPLLVELATGQIISEPPADKRLDPARFDSKAIAR
                     DPALSRTIPSADDLAHIGRQPKYMVVYEVVGNDQAGGVILPVYGKGLWSTLYGFIAFG
                     PDLQTITGFIIYEHGETPGLGGEVDNPLWRAKWVGKRAFDDSGQLKIEVIKGKVDPTS
                     PQSQYQIDGLSGATLTTRGIDQLVKFWLGENGYGPFLRNLQAKRMRNE"
     gene            15817..16434
                     /locus_tag="PLI55_03510"
     CDS             15817..16434
                     /locus_tag="PLI55_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007227783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HPA99938.1"
                     /translation="MNKSLTTLTTPIIKENPVIVQVLGICSALAVTSKLETAIVMALA
                     VTFVTAFANMAVSLIRRQVPGSIRIIVEMTIIASLVIVADQLIKAFAFGVSKQLSVYV
                     GLIITNCILLGRTEAFGLKNAPLASFLDGLGNGLGYSLILLIVASIRELFGAGKLLGF
                     TILRVNTEGGWYVPNGLLVLPASAFFLIGLIIWGLHTLKPETKQE"
     gene            16438..17046
                     /gene="nqrE"
                     /locus_tag="PLI55_03515"
     CDS             16438..17046
                     /gene="nqrE"
                     /locus_tag="PLI55_03515"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HPA99939.1"
                     /translation="MLEHYLSLFIKSVFIENMALAFFLGMCTFLAVSKRLETAVGLGI
                     AVVVVQTITVPINNLIYHNLLAEGALSWLGIDLDLTFLGLITYIGVIAAVVQILEMVL
                     DKFVPKLYHSLGIFLPLITVNCVILAGSLFMVERDYTFGESVVFGIGSGIGWALAIVA
                     LAGIREKLKYSNVPSGLRDLGITFITAGLMAMAFMLFSGIQL"
     gene            17060..18289
                     /gene="nqrF"
                     /locus_tag="PLI55_03520"
     CDS             17060..18289
                     /gene="nqrF"
                     /locus_tag="PLI55_03520"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HPA99940.1"
                     /translation="MSSLVIILIAVGVFIFIVLALVGLILLAKSYLVPVGTIKININD
                     DPDYSFDAPVGGKLLNTLAEKEIYLPSACGAAGTCGLCKVQVLKGGGTILPTELAHIN
                     RREARAGVRLACQLAVKEDLWLSIPTEIFAIRKWICTVRSNRNVATFIKELVLDLPPG
                     EQINFRAGGYIQVETGPHRVNFKDFVIEEPFKSEWDRCNMWRYVSIVDEEVVRAYSIA
                     NYPDEKGIIMLNVRIATPPPEKPDAPPGKMSSYLFSLKPGDRVTISGPYGEFFAKETD
                     REMVFIGGGAGMAPMRSHIFDQLKRLHTQRKISFWYGARSLKELFYQEDFDALQREFP
                     NFEWHVALSEPLPEDNWKGYCGFIHEVVLNNYLINHPAPEDCEYYLCGPPLMVSAVLK
                     MLDDLGVEPENILYDEF"
     gene            18343..18513
                     /locus_tag="PLI55_03525"
     CDS             18343..18513
                     /locus_tag="PLI55_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99941.1"
                     /translation="MLIYFLLILGCTTLAFALMMAALKFSRYKGEKHSGQCGAECHCS
                     YDKKRTCVKPNR"
     gene            18722..19744
                     /locus_tag="PLI55_03530"
     CDS             18722..19744
                     /locus_tag="PLI55_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99942.1"
                     /translation="MISLTLLLGLCFSNALAVKFSWSELISDTLYISTYADALTLTGF
                     PAIPVNDERAESPTILGIQQIKKWHYIPVDQYIALNEPLEIQMSKFFQKFQVTGNQTL
                     VIDHLSLWYDASPAFGAARTLNGYTRLVDSSGETVRDWQWELREKTAKNQKPADNIAI
                     LLNKWMIAQTESLQRRPASDLPVSPYRYRRQMIPWTDIISLGDGYIVIAHLALEYPAD
                     QMVKFIRGSPGIYYRKSSKHESIAIGGRDQQWYFRLNRSWLARLNTTVRVGFNNFNPR
                     EYDYVDFWNIMLINLGFNASLEYRPIYHKGFFVGAGLHQAVNLLPSVINTLETGLLFS
                     IGLVLP"
     gene            19741..>19942
                     /locus_tag="PLI55_03535"
     CDS             19741..>19942
                     /locus_tag="PLI55_03535"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPA99943.1"
                     /translation="MKKIFSLWLILVTGLQAANISGYIVDEKTGEAIIGVNVLIANTG
                     LGTASDLRGFFVVRQVPAGLQTL"
     assembly_gap    19943..20042
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20043..22208
                     /locus_tag="PLI55_03540"
     CDS             <20043..22208
                     /locus_tag="PLI55_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPA99944.1"
                     /translation="DLRGFFVVRQVPAGLQTLRFSHISYAERELRIEVSDKALFLGTI
                     KMTARALPTSAVEVIGQRRTLIEKEMDIASFQVDPIVLLEAPQLRDDVFQLLKFSPSV
                     SMSDPLSPQLHVRGSDPGENLVQLNGMTIYNPQHFLSSQAIFNPYSIKDIEMLVGGFD
                     AEYGGRNSSILNITTREGHQSEVHGEFWPSTSGISGAIEFPVKKNATALLSGRLISDP
                     AYRVLMNSPNLMSDFVGSYLSKIGRTQLRLTAFYARDYLDYSLDNLMLYFPYDFYENY
                     QEGLITNTENRAVGIQTHTLIRTNILLETHFFHSGSRADNDSYVNFVFPDTVNNLDRI
                     IKYHTSIRNSVQDYTAKASLSLYTFWNQTFKFGWEQSHLSFLNKIGGQTDTSARRIGA
                     DLQAVYIQDKIEIGNLALKFGGRSSRQMSSESWHFEPRAALALRLGITTVKAAWGHYY
                     QYLTTLDSRNREFIQFLDYYYNLENQKPVESIHYILGVEGFLTDKLEYSVTGYYKDLT
                     RLYRLEYDAAKKSLELSEILEAGRGKAYGIEWLLRGEIERLSGWVSYTFSHGTRSYPS
                     LQDGRWYYYEGDQPHNLKLLLMYKLTPAITASTTFQFSSGYPRTWETGRYNQYFYNPL
                     ENSVGGFPRDITPVKNNVRFPPRIVWDIGWKKKLREGFGQHLSEYLGLKEAYMTMTIR
                     NLLFLRRNPYYYFYFPGYGYYAFGLEYFPSVNFGYTLRF"
     gene            22211..23005
                     /locus_tag="PLI55_03545"
     CDS             22211..23005
                     /locus_tag="PLI55_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HPA99945.1"
                     /translation="MKIFKNNLLIILASVIAGLAGWQSCDLPWQPGPMPDAIIETEFV
                     PGLNILGIVRLEDTTASSFVYIERAYASNEYETWVDSLPIIKNANVWIAGTRDTTGYI
                     FRYYGEQSDHKYINTDFRPVAGETYQLTITAPDLPDLSASTSVPHLPTIDTGSVVISD
                     GIVNFNLRTTADAYLYDVYLLCENGQLVNRLSNDGAIVKELQFKLNNNLGAPLAIQVY
                     GYDENMATILNATSSIIPQSFLETLSTVEGGYGCFGAVSVLTYVLP"
     gene            complement(23073..23210)
                     /locus_tag="PLI55_03550"
     CDS             complement(23073..23210)
                     /locus_tag="PLI55_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99946.1"
                     /translation="MKIKKKILVLIIIFLLMVASLAISIRSNRQSDIPARESANLKIG
                     N"
     gene            23255..23788
                     /locus_tag="PLI55_03555"
     CDS             23255..23788
                     /locus_tag="PLI55_03555"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPA99947.1"
                     /translation="MKVNLCRATNLRYDLTFITFAIQPNHCYFSAMKILIIHGPNLPF
                     LGKISAVTGSRLTLDKVNTFLRRMAQESGIELKIFQFYSEEKILKAVARNRSDIAGLL
                     ISPGALAINCHTLRELLSILKIKTVEVHLSEMPDAKASFGQSALRDIAVARFLEPGLT
                     AYQKGLQYFIKTKDASQ"
     gene            23814..>25345
                     /locus_tag="PLI55_03560"
     CDS             23814..>25345
                     /locus_tag="PLI55_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPA99948.1"
                     /translation="MKKLSVLSLLFFLPVALAAFTGEIIKTYNLPSTCPTGLTYDGQN
                     LWLADRKTDQLYCLDKNDGKVVRQLPAPGYWPMGLAWDGKALWVSDLRDGKIYQIDPK
                     SGLVIKTIYAPGGSPRDLAWDGKYLWFADDGSDEIIQFSPDDGTTIKSFRAPSTEVRG
                     LAFDGHYLWADDRGKDEIYMIDPRSGCVLIVTTAPGPYPKGLAYDGQALWAVDYQTDK
                     LYQLKVQDDQKFIRKNERKAKVTYTHQTRNFGPGKIRNLDVYIALPQNRDSQKLNGNL
                     QFTPKYDKIVTDRWGQPSAYFNLKNVEAGKIINLEMVTNVSVFEVQYFIYPEKVGGLE
                     TIPADIKQKYLENNEKYQYDHPIIQNAVREAVGYEKNPYWIARRIYNYLIDKMYYELV
                     GGWNTAPTVLERGSGSCSEYTFVYIAMCRAAGLPARYVGSVVVRGDDASLDDVFHRWV
                     EVYLPNYGWIPVDPSGGDNESPRDQAAYFGHLANRFLITTESGGASETLGWYYNSNEF
                     FTT"
BASE COUNT         6794 a         5564 c         6029 g         6858 t
ORIGIN      
        1 ggtttttttg agggaattgc tgagcgagga tgggagtatt tatgtgcatt tggattggaa
       61 gaaggggcat tatgtaaagg ttttaatgga cgaaatattt agtgagacta actttaagaa
      121 tgagataata gttaagcgcg gacaaaaaaa tgttcaagct caatttacga ctatagattc
      181 attggcaagt ggtcatgata ctttactctt tttttcaaag caatcgggaa ctagattttc
      241 aatattaaaa tcatcacttg acgagatgaa gcctggagca tggaataatc actggcgtgg
      301 aactgataga cccgctatgc gatacgagct atttggaatt ttaccggata gaggccaatg
      361 gcgatggtca aaagaaagaa caatgaaagc agttgaaaat tatgaacggt atgtaaatca
      421 gtattctaaa acgatgtcta ttgatgaata ttacgtacat attttagaga caactggaga
      481 aaaactcaac tttgtccgtc tttcatcaac tagaaaacca gaacactatg taccccctac
      541 agagcatcaa attttaagtg atgtttggat ggatgtcact gcttatgaag ctagaaagga
      601 ctatcctaca caaaaaagtg aagaattgct gagtagaata atttgcggtt tttccaaccc
      661 cagcgacctt gtgctggatt gctttgccgg ttcaggtact accctggctg tggcggaaaa
      721 acttggcaga cgctggatcg gggtggattg cgggaagtta gccatttaca ctatgcagaa
      781 gcggctcttg aacatcgctg aaagcaaaga cctggaggat tcgcagaaga agaaaaaata
      841 cggcaagcct tgtaagcctt ttacgcttta caatgccggt ctctacgatt ataagatgat
      901 caaggaactc ccctgggagc agtatcggga ttttgccctt aagctctttc agtgtcgcga
      961 tgaacggcac gagattgccc gcatcgaact ggacggctac ctcggggcgg acagcgttat
     1021 ggtcttcaac tatcagaaat atcctgacgc cgtactggat cggggcttta ttgacgatct
     1081 tcacgaatac ctgggagacc ggattggcaa gcgtttcttt atcatcgctc cagccgcctc
     1141 ggtgcagttc ctcgaggatt atatcgaaaa gggtaatacg aaatattaca tcctgcgcat
     1201 tccctattcc attatcgagg aaatccaaaa ccgcggtttt agcaaaatca aacaaccggc
     1261 gagcgagatg gatgtcaatg ataccgtgga agcagtaggt tttgatttta tccagacacc
     1321 gatggttgaa tgtaaatatt gcctcgataa ccggaaaggg caactggact ttggaaaagg
     1381 atcgagggat tgtgttatcc gaattgagga gttcgagagc cgggtcattt cccgcaaacc
     1441 gctggacttc gctaatctgg agacgctttc gatggttatg ctggattatg atttcgatgg
     1501 tgaggtcttt gatctggatg aggttttcta cgccgaagaa ttgaagaaaa acaattatga
     1561 actgcgcttt gccgaagata aagtgaagga taagattatg attatctaca ttgacatctt
     1621 tggaaatgag aagcgagaga taaagagctt gaaggatttt aagaaaggaa tttcttaaaa
     1681 tggagatcaa tacaaatact caaaactatt ccactctcat taccgatctt acttctctca
     1741 tcgagcaggg acgccaggta gcagttcggt atgtgaatac tgccttggta actacttact
     1801 ggctcatcgg ccgcagaatt gtggagtttg aacagaaagg gaaagagcgg gcggaatatg
     1861 gggcggcttt gctcaagcat ctttctgacg atctaaccaa acaatttggc aaaggttggg
     1921 gcgaacctaa tttgagagca attcgacaat tttatatgat ttatggagat attggaaaac
     1981 gctacacagt gtgtagcgaa tctacaaagt ctgatggaac tattatccga cacacatcgt
     2041 gtagcgaatt ggttccttcc caagaaattc catcagaact tcagacattg tctatagaaa
     2101 ccttccataa cttgtttcct ctttcatggt ctcactatcg cctgcttatg cgtcttgatg
     2161 agccattcaa aagggaattc tacgaagccg aatgtattcg cggcaactgg tcggtcaggc
     2221 aattggaccg ccagattcag tccttgctct atgaacgaac ggcattgtcc agacgcaagc
     2281 tggccgtcat cgccaaagct cacaaaaacc ctatcattct caaacccgag gatgaaatca
     2341 aagatcccta tattctggaa ttcctcggtt taaaagacga gtattccgaa tcacaattgg
     2401 aagaggcttt aatcaggcat ctggagcatt ttcttcttga attgggagtg ggtttcacct
     2461 ttgtcgccag gcaaaaaagg atagacctcg aggggagtca ttatcgtctc gaccttcttc
     2521 tttatcaccg aatattaaaa tgtcttgtgg cgatagactt gaagatcggt gagttcggtt
     2581 ataccgatgc ggggcaaatg aacctttact tgaattatct gaaagacaaa gaaaaatttc
     2641 ctgaagaaaa cgacccagta ggcttgatcc tctgcaccga taaaaagaaa acggtagttg
     2701 agtacgccct gggtggaatg agcaacagga tatttgcctc gaagtataaa ttgcagttgc
     2761 ctgatcccga agttttggaa gctgaaataa cgcgcgaaaa acagagattg ctggaaatga
     2821 aaattgctgg tgaagaaata aagataaaat aaccatgctt gaaaaacaga catttctaaa
     2881 caaagattta gttcttcgag tatctcccag ctacgacccc cgaaagtttg agccaaataa
     2941 atacgaggct tttctggatg ccctctgcgg aaacagggag tatcagaaag aagcaatccg
     3001 gattactttg cgctattttc tgggcggaga ataccaaagc ctcagagagc tggcagagga
     3061 aaattatcac agtaatccca aacttcagga gaaatattcg tctttcgagg atttcagccg
     3121 cgtcttgcaa ctgccggatc aactctccgg ttctctggat catgccactg ccaccggcaa
     3181 gagttatgta atgtatggta ttgctcgcat tatgcttgcc gagggcgctg tggatcaggt
     3241 gctggttctt tgtccatcta ataccatcga agctggttta aaggagaagt ttctcagcct
     3301 ctccgccgat aaaacgctta aagaccttct tccttccgac tccatagttg ctaatccccg
     3361 cattatcaat gcctccaata ccattcagcg aggcgacatc tgtatcgaga acatccacgc
     3421 tacttatatc aatacgaaat ccgctataga agacagcctg gtcggtaaag gtgaacggac
     3481 tctggtactg aacgatgaag cccaccattt aatgaatcct tcggatacgg cgcttaagaa
     3541 atggaaagaa tttttactcg attccaaata cgggttcaag ttcattgtaa atgtttccgg
     3601 cacctgctac atagggtctg aatattttac cgatgtcata caccgttttt ccttgaagaa
     3661 ttccatcgag gaaaagttcg tcaaatctat ccgttatgtg gcggaggatt catccggaag
     3721 tgaggacgag aaatttcaga agatttacga aaatcatatt gagaacaaga cggttaaata
     3781 ccgtgaggtt aaaccgctta ccattctggt taccaaagac attgcggcct gcaaacgcct
     3841 caccgagaaa ctgactactt tcctggccga aaaagaaaat atcagcaaag agcaggcggc
     3901 agaaaaggtt ttgatcgtaa cctcagctaa cgagcataaa aataatattc ccatcctgcg
     3961 acgggtggat gacagggaca atccgatcga atggattacc tccgtcagca tgctttccga
     4021 aggttgggat gttaaaaatg tctttcagat cgtacctcac gaggagcgag catttaattc
     4081 caaacttctt attgctcagg ttttaggacg gggtcttaga attccgccag tctataaggg
     4141 cgagcaacct attgttacgg ttttcaacca tgataaatgg tcaagtaata ttaaacatct
     4201 cgtggatgaa gtcctggaga ttgaaaaacg cttatattcc tacccggtcg aaaagaaagg
     4261 ggattataat tttgatcttt ataacattaa ttacaagcga accgaggaga aagtggaata
     4321 tcctcaagaa aatccttatg aactcctgaa gaaagaatat atcgcctact caagtcaagc
     4381 agaagttgtg ccggaggata ctgtatatgt aactgccatt aatagagcaa tggaaaccgt
     4441 aacatactcc attacgcata aattgtatag cgtcgaagaa atcgcgcaag atgtcttcaa
     4501 tcgccttctg atttttgatc aagaggccga aactgagtac agccaaaagt tcactaagca
     4561 aaagattgcg aaaatcatca gaaaatctct tgcggaaatc caagatgaat ccggcaaggt
     4621 aaatgagaca aaccggattg ctaccttaaa ggcgttcggt gtgattaaaa gaaaagggac
     4681 aaagagccta agattcaaga tcgaggccca gaaactactc aaaataagca ctaaagagat
     4741 tagaaaaaat tccttaggag tggggtctct tcgccatgat agtacggtgt tttgggatga
     4801 ctactctata actcttggag aatcggctga tataaagttg ctgaaagaga ttgaggagga
     4861 tgaaagcctt ccaagatcag ctataatcaa agtcgggaat aaatacaatt tcaagactcc
     4921 gcttaatgtg gcttttgctt ctcatgagcc tgagcgcagg tttatccggg gattgacatc
     4981 cgataaggtc tctaggacaa taaatgcctg gatcaagtct ctcgatgtcg gtttttatag
     5041 tattgattac tcctggcgca agggcgagca tcccaagcag ggaagtttca atccggattt
     5101 ctttatcaag atcggacaag atattttggt aattgaaata aagtcggacg acgatatttc
     5161 cgacgaaaat agggctaaac tgcgctatgc cagagaacac tttgccagag ttaacgattt
     5221 acaaaaagag cagaggtatt acttcaagtt cctttcaccg gcaagtttcg aattattttt
     5281 ccagatgttg agcaacggca cttaccagga tttcaagtcc gagcttgagg caaagttaga
     5341 aaacagggat tagttgattt tcatcctttg cttttgcacc ctagccggga gcttaactct
     5401 ttaccagaat cacctttcta ttaagataaa cgaaggcttg tgatcacgag ataggcttta
     5461 gctttaacag gaatcccgac tactcggaga tttttatcca gttagttttg attactgaaa
     5521 ataagttttt aaaattggca ataaatcgta gatttttgtt cattatgaaa ttctaactaa
     5581 ggagtattag caaaatgttt gggaaaggaa ttcgcagatt atttttcgga ttgagtgttc
     5641 tgctggtagc aatggcttat ggggatagta acattgtaat taatcccgga tttgaagatg
     5701 gaaaagaggg ttggtttgat cgcacttgcg ccatagaaat tgtaacctcg ccggtatatg
     5761 aaggtcgcta tagcggaaaa gcatttcagc gattagctaa ctggcagggt atcaagcagt
     5821 cggtatttgg caggatggtt gacggcaaaa cctatcaagt ttccggctgg gtcagattag
     5881 ataatgcgcc cagtgacgaa gtcgccattt cttttgaaca acaagacgac agcggaacaa
     5941 aatacatcgg ggtcgctcgc gggatagcca ccgatactgc ctggactaaa ctttccggtg
     6001 aatttacatt aaatgtgact ggtactttgt cggttctgga tgtgtatttc gaaggaccgg
     6061 cgccgggcgt caatttctac gtggatgatg tagtcgtata tggtccggag gcgaatccac
     6121 cggtaatcat tccacccacg ccgcaaagca aaagccaggt ggatgccaaa atccgccacc
     6181 aaataataga aggcttcggc gcttctggag cttattatac aatgaatcta gtaaatcata
     6241 ataaaaagac ggaactgtat aacctgcttt tcaaagaact cggactggac attttccgca
     6301 ttcgcaataa ttatgatatg gaaccgcatt caatgcagga aaccgtcgaa attatcaaaa
     6361 acgctaaggc actgcgggga gacaatttga aaattttgat gtcttcgtgg tctccaccag
     6421 cctatttgaa gagtaataac aatgtggtag gtggaacgct caaaaaggtc gccggaaaat
     6481 tcgtttacga aggatttgcc gactggtggt atgaaagtct gatggcttat gccaatgccg
     6541 gagtaactat agattatatc agcatacaga atgaattgaa ttacgaagcg ccttgggaat
     6601 cgtgtgtatt tgctcccagc gaatccgacg ataccacgct tgccgctcat aatgtagcct
     6661 acgaaaccgt ctggcaaaag ttgaacgctg aaatgggcaa aaccatgccg aaaatgctta
     6721 cgcctgaaac ttctggcctg ggagattctg aagtatatat caaaaattta atagatttgt
     6781 cgcatgtcta tggctatgct catcatcttt acgattgtag tggatgtggg gcagatcccg
     6841 atcgcttcat tccgcggatg attagttatc gggaaatggt caagaaatat ggcgccaaac
     6901 ccattttcca gacggaattc gaggacgacc ccggcgcctg gccggatgcc ctcaataccg
     6961 cgctattgtt gcataattcc ctgacagtag agcaagtttc tgcctatctg tactgggatt
     7021 tattctgggc gcccggttcc ggtctgatca gtttaaatga tttcgatact tatacgatca
     7081 agcctaccta ttacgccttc aaacagtatt cagcttttat tgacgctaat tggcagagag
     7141 tcgaagtcac caatgataat accggcctta gaatttccgc gtatgtcagt ccggataata
     7201 aaaaaatgac gatcgtgctt ctcaatataa ctcaggatat ggacattaaa cttaaacttg
     7261 cgatcaaaaa ttttgccagt gccgaaggtg caatctatcg caccagtgaa accgaaaact
     7321 gcgtctacgt tggtgaattc aataaaagaa agcccttagt attgccagcc aactctatca
     7381 cgaccctctc gctttctacc gcggaaaagt aactgagatt aactgccaaa gagcggatat
     7441 ctctcaaatg tcgcttcaca gttgaaaaat ttgattttcc caatatgaaa ggagaaaatg
     7501 ctatatgaaa tcggtaagac acactatcgc taatggcgga cttaatcgcc agcatcttca
     7561 atatcaaact tctgcccttg tattggtaat tcttttaatt atgggatttc tttcctttgt
     7621 ttctgctgaa ccgaccaaat gggtgggcac ctggagcact gccccacaat tggtggaaac
     7681 aaacaataat ccgccaagcc ccggcttgag taataatacc ctgcgccaaa ttgtccgcgt
     7741 ttccatcggt ggtgacaccc tgcggctacg cttttccaat gaattcagca ccaaaccggt
     7801 cacgatgaat tcagttcacg tcgctatctc aaccggcggt ggtgcgattg ataccgccac
     7861 ggacaaaccg ctatttttca acggctctcc gaatgttacc atggaagcag gtaaagccgt
     7921 tacctcagat cccttccaat tcaccttgcc acccagggcg gacattgcaa taactattta
     7981 ttttggcgca acttctccgg acgtaaccgg tcaccccggc tcgagaacta catcatacct
     8041 actcaccggg aatgcagtta ccagagcaga ttgcgccggt gcagtcacaa ctgatcactg
     8101 gtatgttatt aataccattg atgtaaaagc gccggactca gccgcggcgg tggttatatt
     8161 gggtaattcc attacagatg ggcgcggctc cggcacaaac aaacaaaacc gctggccaga
     8221 tgaactggcg cgtcgctttc aggaaaatcc ggctacccgt ttcgtagcgg ttttgaatca
     8281 gggaattggc ggtaattgcg tgctcagcgg aggcttggga ccgaccgcgc ttagtcgctt
     8341 tgaacgcgat gtactcaatc aaagcggagt gaggtggctg atcattttcg agggtataaa
     8401 tgacattggc aacggggcgc ccggcgtagg caaccaacta attgacgctt acaagcagat
     8461 gatagtaaat gcgcattcca gaggtatctt tgtgtacgga gcaacgttat tacccatgaa
     8521 gggctcattt tattattcta cagctcgtga agccgagagg caaattgtca acgattggat
     8581 aagaaccagc ggtttcttcg atggagtcat agatttagat cgggcactgc gcaatccggc
     8641 agatacttta agtctattac ctgaagcgga ttctggagat cacctccacc ccaatgaagt
     8701 cggacatcgg ttgatagccg aagcggtgga tctaaacctg ttcataggca gagaacccct
     8761 ggatcttacc gatgaaagtg agagaattta tttcgagccc gaatgtgcta tcgtaggaga
     8821 aaattgggat atattgaccg actctcaggc ttccaatggt aaatatgtga ccgtcaaagc
     8881 cggaatgcaa agtctcgata gcgcgccctc cggtgacaca aacgccattc atattccttt
     8941 ttcggttgat agtgccggaa gttattatct gtatgcgaga ttaaactgcc cctcctatga
     9001 tgatgattct tattgggtaa aaatggatgg tggtaatttt caaatgttta acggattagt
     9061 gacgaccggg tgggaatgga agaatttaag ttcttacact ctgacgaaag gcgagcataa
     9121 acttacgatc acttaccgcg aagacggagc aaagctggat aaaatttgtc tttcaaattc
     9181 cgcctacgcg ccgattggta tgggagacga ggccgaaaac atttgtagtc ctgccggaat
     9241 gggactggat aattcactca aagtgcccga tcgctatgct ctcagatcaa attatcccaa
     9301 tccttttaat cccaccacga cgatcgctta tcaattgtcc gagacagcct ttgtcagact
     9361 tgctgtatat aatatgaatg gtcaattagt cgaaaacctg attgctaaag aacaatcggc
     9421 cgggtattac gatactgtct ggaacgccac gaattatagt tcgggaatat acttgtataa
     9481 atttcaggca ggcaattttc aacaggtcgg gaaatgttta ttattgaaat aagtcctcaa
     9541 aatacctgac tgtttacgat ctcaaattgt tggggctgta gaatatttgg ttttaccttt
     9601 cctcaaaaat taccgatggt caggcaactt aaaactgaat ggggaagggt ttggatacgt
     9661 gatgttttct ttcgagataa agcgctttag aagttcggat taacccaaat cgtcggacga
     9721 caacttaagt caagtgctac cttttttcct ctgcttatct tttctcaggc ttgacctttt
     9781 tttcgagtct ttgtggcata agattaaacc gctaagttcg ctaagagacg taaaggaaaa
     9841 tagaaaaggg ttgtgaatct ttacgttcat tgccttgatg ggacggaact tgctcgaaat
     9901 aatagctaaa aacaagtttt tcactcccgt accccgtaat ctattttctc aactacaggc
     9961 ttaacctcaa aatttcccca gcaggcttga gtcaagtcga attatagatt cacatctcaa
    10021 tgcctagtcg ggcggcaatg cccttttgat aataatgctt gatttcgcgc atttcagtta
    10081 caagatcggc ggccgctata attcgcggat cggcattccg tccggtaatc acgatctcga
    10141 cctgctttgg ccggttctgg acgaccttga gcaattcatc aacgctaaat aaattgtaat
    10201 agactgcgat attggcttcg tccagaatca ccaagtcata atccccggat tgaataaccg
    10261 attccatttc gactaagcca ttttgtgcca ggcgaatgtc ctcttcatcc ggtttatctt
    10321 tgataaaaca acgccgtcca tactgtttaa tttcgatgcc cggggctaat tggcgcaggg
    10381 ctttgatctc gctatactcg gcgcctttga ggaattgccc gatgaaaacc ttcagcccgg
    10441 caccggcggc tcgcagagcc aagcccaaag ccgcagtggt tttgcccttg ccatcaccag
    10501 tatagacttg gattaatcct tgcattctaa acctttttgt ttgaaatcta ctcaaaattt
    10561 aattacttat cggttaacaa atgactacta aaatgaaatc aaatgcaaaa gtgcaaattc
    10621 gcctcgaacc gctcgggcga gtgattgaag ttgacgccgg atcggctttg aaagacatcc
    10681 tttttgagca aggagtagaa tttccctgcg gtgggcgcgg cgtttgtcgc aaatgtcgca
    10741 taaaaatttt aaagggtaac ctgaaagtca atgagattca gaaaaaggcg ttgacttccg
    10801 aggaaattag ccagggctgg cgtttagcct gtcaatgtca ggtgacggaa ccattagtta
    10861 tcgagctcgc ccagctggaa atcccgatcc tttctgatgt cacccggata aaactcgtcg
    10921 gagaaagcgg cttggcggtg gtggtagatc tcggaacgac cactctggta gcccaattgc
    10981 ttgatttatc cagcggaata gttctgggag tggaaacggc actcaatccg caagcgcgcc
    11041 atggcgccga tataatgagt cgcatccagt tcggcttgac aaccgcaggc cgagaattat
    11101 tacaaaacct gattcgtcat caaatcggac agatgattac tcgcctggta gaaaaatgtc
    11161 gctgtccgga gcggcgactg aaacagatta atatcgtcgg taattccgtt atgcaccact
    11221 tgtttggcgg gttgtcggtt gaaccgctgg cgctattgcc gtttgaaatc gagcaacctg
    11281 gtgaattgga atggtcggca gaggaaatcg gctggcagtt gccgggcaat cccaaagtaa
    11341 aatttctacc ttgtctcggc ggattcgtgg ggagtgacat tctagctgga attctggcgg
    11401 ttggaatgca ccgcagtccc gaaaccgtcg ctctgattga tctcggtacc aacggcgaaa
    11461 ttgtcattgg taatcgtgac agaattctgt gtgcttcgac tgcggctgga ccagcgtttg
    11521 aaggtgctca gattacgatg ggtatgcgcg ccaccaccgg cgccatctgg aaagtttggc
    11581 ttaccaatgg ccagttgcaa gttgaagtaa tcggcaatgt cccggcgcgc gggatttgcg
    11641 ggagtggact agtggacgcg gttgccgccg cactcgatct tggctggatt gatccaaccg
    11701 gacgaattac cttgcccaaa cgagaaatcc cgctccagaa gccggtcgtc attacccagt
    11761 ccgatattcg tgaattgcaa ttggctaagg gcgccatcgc ggctggaatc cagattttac
    11821 ggaaacaaat gcaattgaaa ccggctgata taaagcaagt ttttctggca ggtgcattcg
    11881 gcaattacat caatgtcgtc agcgcgcggc gaatcggtct gtttgatttt cccacagaat
    11941 taattgaaca agccggaaat accgctctgc acggcgccaa gttgtcttta cttaatgagc
    12001 aagagtacca ctcgattccg cttattacaa aacactttcc actagctacc gacgctcaat
    12061 ttatggaaat atttgccgag aatatgaact ttccggggtg agccacgatt tccagttgcc
    12121 ggttacaatt caccgaattc ctgcgtcccc gcttccaact aagcaaaata atcaaccgcc
    12181 aggaaaacca agatattaag ctctcctaag tgcttataag agattaacgg cgatttgcca
    12241 tcccattaaa acaatagaac ttaacagcac ttctttgcca acaagcaccc agctcattct
    12301 ctataaaatt caagaaattc tgctaaagtt taaatttcac tacataaaag taatttttgt
    12361 gtttgttcaa taaagtaatt ttttttaatc tacgacttat gaaaattcga ctaagattta
    12421 gttcagtata aatacaacag acatttaatt ataccaatcg tatcgggaaa aaaagcatac
    12481 cgctaatgtt aattaaaatt aaaaagggat taaatctgcc aattgccggg gcaccggagc
    12541 aggttattta cccggctcaa tcggtctcgc aggtagcgct tttaggactg gattacgtcg
    12601 ggctgaagcc gcaattcgcg gtaacgattg gcgatcaggt gaaactcggg caaccccttt
    12661 ttagcgataa aacttacccg cagattcgat tcaccgcgcc gggtgccggg caagttattg
    12721 ccattaatcg tggcaccaag cgggcttttc tatcgctggt aataaaattg gatggcgatg
    12781 atgctgttga attcaaatct ttttcagagc aacaaattgg ttctctaacg caagaagaaa
    12841 ttataaatca attgctggag tccggtttat ggccttcgtt caaagccaga ccattcggta
    12901 aattagctaa tccggaaagt caaccgcatt cgattttcgt tacggctatg gacactaatc
    12961 cactggcgcc agacgttgcg gtcatactac gtgaacagtc gaatgatttc cagcgcggct
    13021 tagcactcat tgccaaactg actgctggaa aagtgttcgt ctgccaagca ccggatacag
    13081 cgattcccgt tcctgaaatt gaaaacatag aaatagtgga atttgccgga ccgcatcccg
    13141 ccggactggc aggaacgcat atccatcttc tcgacccggt ttcgctccac aaaacagttt
    13201 ggagcattgg tgctcaggat gtcgcggcta tcgggaaatt gtttactacc ggcagattgc
    13261 caaccgcgcg aatcgtcgct ttggcgggtt cagcggttaa aaagcctcga ctgattacca
    13321 cgcgagtcgg cgcaaaccta agcgaattaa ccgacggcga actggcagaa ggccataatc
    13381 gcataatttc cggttcggtt cttgccggac atacagcggc aggcgagttg aattttctcg
    13441 gacggtttca ccaaattgtg agtgtcatcc cggaaggtgg ccgtcgtaaa ttattcggct
    13501 ggcttagtcc cggttggcgg ctattttcgg tgaagaatat tgcgctggcg gcttttttac
    13561 caaaccgtaa gttggtcttc gacaccgata cgcacggtag tgaacgcgcg atcgttccca
    13621 tcggtagtta cgaaaaggta atgccattgg acattttgcc gacttacctt ctgcgagcgt
    13681 tggcggtaga tgacctcgaa gaagccgaaa aactcggttg tttagaactg gttgaggaag
    13741 atttggcgtt atgtacattt gtctgtccct cgaaaattga ccacgggcag aacctgcgac
    13801 gcgtgttgaa tttaatcgag aaggaaggat agtgggcggg ttccggaaat ttctcgatca
    13861 ggcaggcaag ccttttcgac ccggcgggaa gcttgctgga ttatattacc tgttcgaagc
    13921 gattgacgcc tttttctata cgccgggcaa agttaccaaa ggcacggtgc acgtacgtga
    13981 ttttatggat ttgaaacgga tgatgatcac ggttttcgtg gcgctgttgc cggcagtttt
    14041 catggctttt tataataccg gtctgcaggc taatctcgta ttgcagtcgc tcggctccgg
    14101 cgctaatttt acctggcaac aacgcctgat gctggcattg ggactggact tcaatccgga
    14161 aagtttctgg tccaatttca tccatggcgg tttatatttc ctgccggttt atatcataac
    14221 gctgatagtt ggcggattct gggaagtgct gttcgccgta attcgcaaac atgaagtcgc
    14281 ggaaggtttt tttgttacca gcctgctttt tccgctgatc ctgccgccga cgattcccta
    14341 ctggcaggta gcattgggaa tttcattcgg tgtagtgctc ggcaaggaag tattcggcgg
    14401 ggtcggcttc aatgttttca acccggcatt ggtggcgcgt gcttttctgt tttttgctta
    14461 tccggcgcag attaccggtg acaaggtttg gatcgcagtg gacggcatta gccgggcgac
    14521 gccgctggcg cagttcgcgg aaacgtcgtc ggcagttact accagctggc tggatgcttt
    14581 cctggggttt attcccggtt caatgggcga aacctcgacc ttagcctgtt taattggggc
    14641 gataatttta ttggttaccg gagtaggtag ttggcgcatt atgctgagta ttttaatcgg
    14701 aatggtgaca ctttctacta ctttcaattt aatgggcagt gccactaatc caatgttcgc
    14761 ggtgacgccg ctctggcatc tggtgctggg cggatttgct tttgggacag tttttatggc
    14821 gactgatccg gtttcgggcg cagttaccaa accgggccaa tgggtatatg gtttactgat
    14881 tggcgttatg gttattatgg tgcgcgtttt gaatccggct tttccggaag gtatgatgct
    14941 ggcgatttta ttcggaaata tgttcgcgcc gctaatagat cgcattttta ttaacgctca
    15001 tataaagagg agagcaaccc gcgatgtcag atgaaagcgc gcggaaaacg ttcttagtag
    15061 cactgggcgt ttgcctaatt tgcgcggtga tggtggcgac ttctgtggtt ctcctgcgtc
    15121 cgcgccagca agtaaaccaa gaattggaca agttgaaaaa tattctccaa gccgccaacc
    15181 tttacaccga tgacgccgcc gtgaaaaaga cttttcagga gaaaattcgc ccgctactgg
    15241 ttgagcttgc caccggtcaa attattagtg aacctccggc ggataagcgc ctcgatccgg
    15301 cacgctttga ttccaaagcc attgctcgtg acccggcctt aagtcggaca attccatctg
    15361 ccgatgattt ggcacacatt ggtcgtcagc caaaatatat ggtagtttat gaggttgttg
    15421 gaaatgatca agctggtggt gtgattctgc cggtttacgg caaaggtctc tggtccacgc
    15481 tctatggttt catagctttc ggtcctgatt tacagactat caccggtttt atcatatacg
    15541 aacatggtga gacacccggt ttgggcggtg aagttgacaa tcctctctgg cgcgccaagt
    15601 gggttggcaa acgcgctttc gatgattccg gccagttaaa aatcgaagtt atcaaaggta
    15661 aagttgaccc aaccagtccg cagtcccaat accagattga tggcttgtcc ggagcaacgc
    15721 tcaccacgcg cggcattgat caactcgtaa aattctggct cggagaaaat ggctatgggc
    15781 cgtttttgcg caatcttcaa gccaagagga tgagaaatga ataagtcact caccacttta
    15841 accacgccga taattaaaga aaacccggtg atcgttcagg tgctgggaat ttgttcggcg
    15901 ctggcggtta cttctaaatt ggagacggcg atcgtgatgg cattggcggt aactttcgtt
    15961 acggcttttg cgaatatggc ggtcagtttg atccggcgcc aggttcctgg aagtatccgc
    16021 attatcgtcg aaatgacgat catcgcctcg ttagtgattg tcgccgatca attgattaag
    16081 gctttcgctt tcggcgtcag taagcaactc tcggtttatg ttggcctgat catcactaac
    16141 tgcattctgc tggggcgcac ggaagccttc ggtttaaaaa atgcgccgtt agccagtttc
    16201 ctggatgggt taggaaatgg gttgggttat agtctgattc ttttgattgt ggcttcgatt
    16261 cgcgaactat tcggcgccgg aaaattgctc ggctttacga ttctgcgggt caataccgaa
    16321 ggcggctggt acgtgccaaa tggattattg gttttgcccg ccagcgcttt tttcctgata
    16381 gggttaatta tctggggttt acacactttg aaaccggaaa caaagcagga gtaaattatg
    16441 ctggaacatt atctgagcct gttcatcaag tccgttttca tcgagaatat ggcattggct
    16501 tttttcctcg gtatgtgcac ctttttggcg gtctccaaac gtttggaaac ggcagtcggg
    16561 ctgggtattg cggtcgtcgt ggttcagacg atcacggtcc ctattaataa cctaatctat
    16621 cacaatttgc tggctgaagg cgcgttgagc tggttgggca ttgacctaga tttgacgttt
    16681 ctcggtttga taacttatat cggcgtcatc gccgccgttg tccagattct ggaaatggtg
    16741 ctggataaat ttgtaccaaa gctttaccat tcactcggga tttttctgcc gctaattacc
    16801 gttaattgtg tgattctggc tggctcgctt tttatggtcg aacgcgatta taccttcggt
    16861 gaaagtgtgg ttttcgggat tggttcggga ataggctggg cgctggcgat cgttgcctta
    16921 gccggcattc gcgagaaatt gaaatatagt aacgttccat ccggtctgcg tgatctgggc
    16981 attactttta ttacagccgg attaatggcg atggctttta tgcttttttc cggaattcag
    17041 ctttagtaga tacgatttta tgagcagtct ggttataatt ttaattgcgg ttggggtttt
    17101 tatttttatt gtgctggcgc tggtcggctt gattctgttg gcgaagtcct acttagttcc
    17161 agtcggcacg attaaaatta atataaatga tgatccggat tattccttcg acgcgccagt
    17221 cggcggcaaa ctattaaata ccttggctga aaaggaaata tatttgccgt ccgcctgcgg
    17281 agccgctggc acctgcggac tctgtaaagt acaagtttta aaaggcggcg gcacgatact
    17341 gccgacggag ctggcgcata tcaatcgccg ggaagcccgc gcaggtgtgc ggctagcctg
    17401 tcaactggcg gtcaaggaag atttgtggct ctcgattcca acggaaattt tcgccattcg
    17461 caaatggatt tgcacggtgc gctcgaatcg caacgtggcg acttttatca aagaactcgt
    17521 tctagatttg ccaccgggcg agcagataaa tttccgggcg gggggttata ttcaggtgga
    17581 gactggacca catcgcgtaa acttcaagga ttttgtaatt gaggaacctt ttaaatcgga
    17641 atgggatcgc tgtaatatgt ggcgctacgt ttccattgtg gacgaagagg ttgtgcgagc
    17701 ttattcaatc gccaattatc cggacgaaaa aggcataatt atgctgaatg tgcgaatcgc
    17761 cacgccgccg ccagaaaagc cggatgcgcc tccaggtaaa atgtcgtcat atttattcag
    17821 tttgaagccg ggcgaccgag tgacaatttc tggaccctat ggagagttct tcgccaaaga
    17881 aaccgataga gaaatggtct tcatcggcgg cggagccgga atggcgccga tgcgttcgca
    17941 tattttcgat caattgaagc gcctgcacac ccagcgtaaa atctccttct ggtacggcgc
    18001 ccgtagtctg aaggaactgt tttatcagga ggacttcgac gctctgcaga gagaatttcc
    18061 gaattttgaa tggcacgtag cgctttccga acctttgccg gaggataatt ggaaaggcta
    18121 ttgtggattt atccacgaag tggttttgaa taattatttg atcaaccacc cggcgccgga
    18181 agactgcgaa tattaccttt gcgggccgcc cttaatggtc agcgccgtat tgaaaatgct
    18241 ggacgatctg ggcgttgaac ccgagaatat tttgtacgat gaattctgat gaactaaaat
    18301 tgtgaaaccg ggacgagatt caagcaaaaa caggtaaagt tgatgctgat ttattttcta
    18361 ttaatattgg gatgcacgac attagctttt gccctaatga tggcggcgct gaaatttagt
    18421 cgctataagg gcgaaaagca tagtggacaa tgcggtgcgg aatgccattg ttcgtatgat
    18481 aaaaagcgga cttgtgttaa gccgaatcgg taatcattaa tttcgcggca gaaacctctt
    18541 cctttcagct tcacacagtt caggcggaat aggcagtgtc agaaaaaagg ggaaaaatct
    18601 tttaatcgcg cctaaaaact ccatcgctct taaagttgca tatttttggc gttggtcgta
    18661 aaatttacac caaccttaat gaaccaatta cttgacgttg aataatcaaa atatcgcaaa
    18721 attgatctcc ttgactttat tgttggggct ttgtttttcg aatgccttag cagttaaatt
    18781 ttcctggtcg gagctaattt ccgatacgct ttatattagc acctatgccg atgcgcttac
    18841 gctgacggga tttccagcta tcccggtaaa cgatgaacga gccgaatcac cgacaatcct
    18901 cggcattcag caaatcaaga aatggcacta cattccggtt gaccagtaca tcgccttgaa
    18961 tgaaccactg gaaatacaaa tgtccaagtt tttccaaaaa tttcaggtta cgggtaatca
    19021 aactctggtt attgatcatc tcagtctctg gtacgacgcc agtccagcat tcggcgcggc
    19081 gcgcaccttg aacggctata cgcggctggt agattccagt ggagagaccg tgcgcgattg
    19141 gcaatgggaa ttgcgcgaga agacggctaa gaatcaaaag ccggcagata atatcgccat
    19201 tctactcaat aaatggatga ttgcccagac cgaatctttg caaagacgac cagcctccga
    19261 tttaccagta tcgccttatc gttaccggcg gcagatgatc ccttggactg atataattag
    19321 tttaggcgac ggctatatcg taattgctca tctggcgctg gaatatccag ccgatcagat
    19381 ggtaaagttc attcgcggtt cgccgggaat atattaccgc aaatcttcga aacacgaatc
    19441 catcgctatc ggcggcaggg atcagcagtg gtattttcgc ctgaatcgtt cctggttggc
    19501 gcgtctcaat accactgttc gggttggttt caacaatttc aatccccgcg aatatgacta
    19561 tgttgatttc tggaacatta tgttgatcaa tttagggttc aatgccagcc ttgaatatcg
    19621 gccgatttat cacaaaggat ttttcgtagg tgctggtctg catcaggcgg tcaatttatt
    19681 gccgtcagtg attaatactc tggaaaccgg cttgctgttc tccatagggt tggtgctacc
    19741 atgaagaaaa tattcagcct gtggttgatt ttggtcaccg gtctgcaagc cgccaacatc
    19801 tccggttata ttgtagatga aaaaaccggc gaagcgatta tcggtgtcaa tgttctaatt
    19861 gcgaacaccg gtttaggaac cgcctctgat ttgcgcggat ttttcgttgt ccgccaggta
    19921 ccggctggac ttcagacttt aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20041 nngatttgcg cggatttttc gttgtccgcc aggtaccggc tggacttcag actttaagat
    20101 tctcgcacat tagttatgcc gaacgtgaac tccgaatcga ggttagcgac aaggcgcttt
    20161 ttctgggaac gataaaaatg acggcgcggg ctttgccgac ttccgccgtc gaggtcatcg
    20221 gccagcggcg cactttaatc gaaaaggaaa tggatattgc cagtttccag gtggacccga
    20281 ttgtcctatt ggaggcaccg caattacgcg acgatgtttt tcaattatta aaattttcgc
    20341 cgagtgtgag tatgtcggac ccgctttcac cgcagttgca tgtgcgtggt agtgacccgg
    20401 gcgagaatct ggtgcaattg aatggtatga cgatctacaa ccctcagcat tttctgagtt
    20461 cgcaggctat tttcaatcct tattcgatca aagatatcga aatgttggtc ggtgggttcg
    20521 atgccgaata tggtggccgc aattcctcga ttctgaatat caccacccgc gaaggacatc
    20581 agagcgaagt gcatggtgag ttctggccat ccacctctgg gattagcggt gcgattgaat
    20641 ttccggtgaa gaaaaatgcc accgctctgc tttccgggcg tctgatttcc gacccggctt
    20701 atcgggtgct aatgaattca ccgaatttga tgagcgattt tgtcggctcg tacttgagta
    20761 aaatcggacg cacccaattg cgcctaacgg ctttttatgc gcgcgattat ctcgattaca
    20821 gtctggataa cttgatgttg tactttcctt acgattttta cgaaaattat caggaaggtc
    20881 taatcaccaa caccgagaat cgggcggtcg gaattcagac gcatacctta atccgcacaa
    20941 atattttact ggaaactcat ttcttccatt ccggctcgcg ggctgataat gattcctatg
    21001 ttaactttgt ttttccggat acagtcaaca atctagaccg gattatcaaa tatcacactt
    21061 caatccgcaa ttccgtacaa gattataccg ccaaagccag cctatcgctg tacactttct
    21121 ggaaccagac tttcaagttc ggttgggagc aaagccattt aagtttttta aataaaattg
    21181 gtgggcagac tgatacttcg gcacgtcgta ttggagccga tttacaagcg gtttacattc
    21241 aggataaaat cgaaatcggc aatctggcgc tgaagttcgg tgggcgcagt tcgcggcaaa
    21301 tgtcatcaga aagttggcat tttgaaccac gtgccgctct ggctctgcga ttgggaatca
    21361 caacagtcaa agccgcctgg ggacattatt atcagtacct tacgactctg gactcacgca
    21421 atcgagaatt cattcaattc ctggattatt actacaatct ggaaaaccag aagccggtcg
    21481 aatctatcca ttacattctc ggagtcgagg gctttttaac cgacaaactg gaatattcgg
    21541 ttacgggtta ttacaaagat ttaactcggc tttatcgcct ggaatatgac gccgctaaaa
    21601 aatcacttga actgtctgag attctcgaag ccgggcgggg caaggcttac ggaatcgaat
    21661 ggctattacg cggtgaaatc gagcgccttt ccggatgggt tagctatact ttttctcacg
    21721 gtactcgcag ttacccatca ttgcaagacg gacgatggta ttactatgaa ggcgatcagc
    21781 cgcataatct taaactcttg ttgatgtata aattgacccc ggcaatcacc gccagcacga
    21841 cttttcaatt cagttccggt tatccacgca cctgggaaac cgggcgttat aaccaatatt
    21901 tttataaccc gctggaaaat agcgtaggcg gtttcccgcg cgatataact ccggtgaaga
    21961 acaacgtccg ctttccgccg cgcattgtct gggatatagg ctggaagaaa aaactgcgcg
    22021 agggatttgg tcagcacctg tcggaatatc tcggattgaa agaggcttat atgactatga
    22081 ctattcgcaa tttgttattt ttgcggcgca atccttacta ctatttttat tttccgggat
    22141 acggctatta tgctttcgga ttggaatatt ttcccagtgt aaattttgga tatacgctcc
    22201 ggttttaatt atgaaaatat ttaaaaataa tttattgata attttggcgt cggtcatagc
    22261 cggtctggcg ggctggcaaa gttgtgattt gccttggcaa cccgggccaa tgccggacgc
    22321 gatcattgag acggaattcg tcccgggctt gaatattttg gggattgtgc ggctcgagga
    22381 tactacggcg agctcttttg tttatatcga gcgggcgtat gcctccaatg aatacgaaac
    22441 ctgggtggat tccttaccga ttatcaaaaa tgccaacgta tggatagcgg gtacgcgcga
    22501 taccaccggc tatattttcc gttattacgg cgaacaaagc gaccataaat acatcaacac
    22561 tgatttccgc ccagtcgccg gtgaaaccta tcaattgacc atcacggcgc cggatttgcc
    22621 cgacctatct gccagcacca gcgttcctca tttgccgaca attgatactg gctcggttgt
    22681 aatttccgat ggcattgtaa atttcaattt gcgcacgact gccgacgcct atctgtatga
    22741 tgtttacttg ctgtgcgaaa atggtcaact ggtcaataga ctctcgaatg acggagcaat
    22801 tgtcaaggaa ttgcaattta aactcaacaa taaccttggc gctccactgg cgatccaggt
    22861 ttacggctac gatgagaata tggcgacgat actgaatgcc acttcgagca taattccgca
    22921 atcttttctg gaaaccctta gcacagtcga aggcggctat ggctgtttcg gagcggtcag
    22981 cgtgctaact tacgttttgc cttaaaagtt ttagttatga caatccaaat aatagaatcg
    23041 gtgtaatctc accgactgta aaaatgcggt ttttaattgc cgattttgag attggcagat
    23101 tctctggcgg ggatgtccga ttgccgatta ctgcggatgc tgatagcaag agacgctacc
    23161 atcagcaaaa aaatgataat tagaaccaat atttttttct ttattttcat aaatctactg
    23221 tgtcaattca agatacttaa attttaatga accggtgaaa gttaatctgt gtcgagcgac
    23281 aaatctgcgt tacgatctga ctttcatcac atttgcaatt cagcccaatc actgttattt
    23341 ttcagctatg aaaatattaa taattcacgg acccaatctg ccctttctgg gtaaaatctc
    23401 agccgtaacc ggctcgcgct tgacattaga taaagtcaac actttcctga ggcgtatggc
    23461 acaggaatcc ggcattgaac tgaaaatatt tcaattttat tccgaagaaa aaatattgaa
    23521 agccgttgcc cgtaaccgtt ccgacatcgc cggattacta atcagtccgg gagctctggc
    23581 catcaattgt catactctcc gcgaactgct ttccattctg aaaatcaaga ccgtcgaggt
    23641 tcacctgagc gaaatgccag acgcgaaggc aagctttggt caatccgctt tgcgggacat
    23701 cgccgtcgcg cgtttccttg aacccggttt aactgcctat caaaaaggct tacaatattt
    23761 tattaaaacc aaagatgcgt cacaatgaaa cctgtcaata ctggaggttt tttatgaaga
    23821 agcttagcgt tcttagccta ttatttttct taccggttgc gttagcggct tttaccggtg
    23881 aaatcatcaa gacctataat ttaccgtcca cctgtcccac aggactgacc tacgatggcc
    23941 aaaacctctg gcttgctgat cgaaaaaccg accaacttta ttgccttgat aaaaacgacg
    24001 gtaaggtcgt gcgtcaattg cccgcgcccg gctactggcc aatgggctta gcctgggacg
    24061 gaaaagctct atgggtctcg gatttacgag acggcaagat ttaccaaatt gatccgaaga
    24121 gcggattggt tattaaaact atttatgcac cgggcgggag tccgcgcgac ttagcctggg
    24181 acggaaaata cctgtggttc gccgacgacg gttcggatga aattatccag ttcagtccgg
    24241 acgacggcac taccatcaaa tccttcaggg cgccttccac cgaagttcgc ggtctggctt
    24301 ttgacggtca ttatctttgg gctgatgacc gcggcaaaga tgaaatctat atgattgatc
    24361 cgcggagtgg ctgtgttctc attgttacga ctgctccagg accatacccg aaaggtctgg
    24421 cttacgacgg acaagcgctc tgggcggttg actaccaaac cgataaactc tatcagctga
    24481 aagtgcagga cgaccaaaaa ttcattcgta aaaatgaacg taaggctaaa gtaacttata
    24541 cccaccagac tcgcaatttc gggccgggca aaattcgtaa tcttgacgtc tatatcgctc
    24601 tgcctcaaaa tcgcgattcc cagaaactca acggcaatct gcaattcaca cccaaatacg
    24661 acaaaatcgt aaccgaccgc tggggtcagc cgtcagcgta tttcaacctg aaaaatgtcg
    24721 aagccggtaa aatcatcaat ttagagatgg tgactaatgt gagcgttttt gaagtacaat
    24781 attttatcta tcccgaaaaa gtcggcggac tggaaacaat tccggccgac attaaacaga
    24841 aatatctcga aaacaacgag aaatatcaat atgatcatcc catcatccaa aacgccgttc
    24901 gcgaggcggt aggttatgaa aaaaatcctt actggattgc ccgcagaatc tacaattact
    24961 tgattgataa aatgtactac gagctggtcg gcggctggaa tactgctcca acggttctgg
    25021 aacgtggcag tggcagttgt tccgagtaca ctttcgttta catcgctatg tgtcgggcgg
    25081 cgggattgcc ggcgcgttat gtcggctcgg tcgtcgtgcg cggcgacgat gctagtctgg
    25141 atgatgtctt ccatcgctgg gtcgaagttt acttaccgaa ttacggctgg attccggttg
    25201 atccgagcgg tggcgataac gaatccccgc gtgaccaggc ggcttacttc ggtcacctcg
    25261 ctaaccgctt tttaatcact accgaaagtg gcggcgcctc cgagacgctc ggctggtatt
    25321 acaattccaa cgaattcttt accac
//
LOCUS       DAOOMU010000023        24946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1875_length_24946_cov_16.729380, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000023 DAOOMU010000000
VERSION     DAOOMU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 24946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24946
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1875_length_24946_cov_16.729380"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1413)
                     /locus_tag="PLI55_03565"
     CDS             complement(7..1413)
                     /locus_tag="PLI55_03565"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPA99949.1"
                     /translation="MNSILIVEDSQDTRENIAELLTMNGYKVRTTDNGQTALRIIKTQ
                     TPDCVILDVRLPDTTGHEILKQIQNEIESGLVVIIITAYGEVSMAVNAMKMGAYDYLE
                     KPFENAVLLMVVKRGLENQQVKRELAHLRQMLGTDQDSEQVLGHSPAIKKVISQINSV
                     AQTDLTVLIQGETGTGKEVAAHLLHNKSKRAKQPFIAIDCGAIPENLIESEFFGFEKG
                     AFTGATQSRPGKFQLAHQGTLYLDEIGNLPLAQQRPFLRVIENKTFRPIGAINESKVD
                     IRLIIASNANLLQLVSENKFRKDLYYRISEYTITMPPLRERLEDIPHLTALFLSEANF
                     ELNSKITDISEEAMAKLLSYNWPGNVRQLRNVIRKAALLATDTITPSHISFPSEFDSE
                     EPEQSFFTVYKNDINRYPNYRTAYAEFTREFEKELIKNAYEKAHGNITKAARIYGIDR
                     RNFYHKLDKYAKDFPKKQ"
     gene            complement(1448..5869)
                     /locus_tag="PLI55_03570"
     CDS             complement(1448..5869)
                     /locus_tag="PLI55_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPA99950.1"
                     /translation="MRSIFKKPEINNEKPAVLRGGRFSRLLILNISTCLLITNAQISI
                     AKTVIKPGKRLHFEKINTEKGLPSNHITSIVQDSIGFMWFATRENGLCRYDGNEIVTY
                     HPEDSVATSLTCQNIKRLFIDSKGALWIIGSVGDIPHKGCIDKYDPLTNGFIHILSPN
                     DSIPSIIDIAEDRLGNFYFSVTDIRQQNNQILKAFNQNGSYQIKKISPLVFDDNSILP
                     ANYVIHRIINKGDGKLLLGLSQYGIVEYDINTGRINEDDFQNLNYYFNRFSFKIDDIL
                     LDCNQNLWIAAHKFLAKYSIADGSLQYYLLPLKPDGSEINPYQDPIKNYYYYTINQLY
                     EGSGGQIWVVGSQCNQNETFFRYDPETEIFWHYSTDPYDENQISVNNINCFFIDKSGS
                     AWLATQYSGVFRFSLPRPFMDLRSISDSINFNNDNVNDVLEDHENNLWFGLKGKVLRY
                     NPKTKNVKIFPLKLSNQESPPPGTLHIYEDSQNTIWVGTTLGLFKLNPSSQEFIYLNH
                     VTKNQPNKQNSIRANHIPAIYEDHEGNFWLGTAAGLCRYDRQTNQRKYYSISGPTKNV
                     PNKNIVYDILEDRNKNLWLATRNGLILFDRNSGTYQLISDSTLIFMPESDEGFYRLYE
                     AQDGQIWLTSSLPYLISYNPLTKKFVYRTENSGYIWKYPITGVIEDHNGIFWFRTTQG
                     IIKHNPILNTFTLFDRNDNVNYYYKDRSTSDIREPSYLKNNILRNYCRLRSGWIMIGG
                     KCGYTYFHPDSINNKYVPNIVITKFKNDYTEKIISNHSPNQITLDLPWNENYFTISFS
                     LLDYYIPAENKYYYILEGYDKDWKKCVKTNSVSYSKVPPGQYTFRVKGENSDGVLSRN
                     EATIRIKIVPPFWKTAWFKILIWVLGLGVVVLINYIRIAVIRHQKEELEKQVVDRTQA
                     LIEKSKALEAAHNQLEEKVQERTQELAEANVELNREINIRMKAEESLKRSEEVARVLL
                     NAPIDLAMLIDPDGNILAANNSVLKHFQASENEVINHNLSEFSPLNLFERRKIFLDKC
                     ISTQSPQEFEDENRGRIFHYHFYPIIGMDQKVNNIAIYIRDITDQREMENFLRNARSE
                     LEKQVNQRTAELRKTNRQLLKEINTRKKIENKLRASEEKYRDLFQNANDLIWTMDKTG
                     KFLSVNNHFKNLSGYSKKELFAINPLTLIHPEHQFRIIRCYLKVLTDGPTGTELNVMT
                     KSGEIRPIWLKMRPIMKNNQIIGIHGIGRDITEIRHAQQELRESEEQKRESMRQFILK
                     LAHEVKNPLASIKSSAQLVASMNNNHNPQVEKHMDIINRNVDTCNKVIRDLFSYAHAT
                     NYQFEEVNTAKFIKSINDFIEEKLNEYPYIKISVNYDRDLPPIYIDEFRLTQAFQNII
                     NNAFEAMTKKGRFTLNVKALKTEKQVQFLFTDTGSGIPAEDLPQIFQSFYSSKSKGFG
                     LGLALTKEIIEFHHGKISVNSILHHGTTFKVTIPAFQ"
     gene            6064..>6744
                     /locus_tag="PLI55_03575"
     CDS             6064..>6744
                     /locus_tag="PLI55_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPA99951.1"
                     /translation="MHQTIKIFLIALTLPTLILAGDLFFSEYIEGSSYNKAIEIYNPT
                     GAEVDLSDYTIKQSNGGSGWGCYTTDDGVITEDTRYILPLSGTLSPDDVYVIYHNDAA
                     SEIKSVGDIGFSYVQTPNGGNGDNVINFNGNDALGLFKDGVLIDVIGVPTENPGEDKG
                     WTVAGTSNATKDHTLVRKSTVSQGNTDWTSSAGTSADDSEWEVHPQNTFTYLGSHVWS
                     GGGNVSPMA"
     assembly_gap    6745..6844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6845..7991
                     /locus_tag="PLI55_03580"
     CDS             <6845..7991
                     /locus_tag="PLI55_03580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPA99952.1"
                     /translation="SPDFITSADEITVSATITPVVGSISSAKIYYGAPGSLINTAEMW
                     KDTGDTWMGNIPPQTGNSIIEFYIYCEDDQTPANSGKSATQTLIIANSTPETIANIHT
                     NIETLNGKMVTIRGIVTIGAGVLASGYTSAYIQDASGRGLNLYEYGKLYSDITRGDEL
                     MVVGYVEKYNTTIEVTNFIYKKISSGNELPPAQVVTVAGANSSDWEGTLIEFSGMVTK
                     QETSGEATNISVSAGTDTTIARIPDATGIDVASIALGNSYKFRGVGNKYKSTYQTLIG
                     YAEDIVPLTAVKESKTNALTFKLNPAYPNPFNPATTISWQLDKNGAYEVVVFNVLGQK
                     IDVIASGYATAGSYAKVWNAAKFPSGVYFVQLQAGNRVRTQKMLYLK"
     gene            7995..9989
                     /locus_tag="PLI55_03585"
     CDS             7995..9989
                     /locus_tag="PLI55_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPA99953.1"
                     /translation="MKKRIILPGLILLPVLLLTAQDHVVISEIVLQPSNAEYIKLYNP
                     TDNAVDLSNYYLTDGTDPSNQKYYHNLPTGTNFWSGSGSDFIVRFPPGYSIEAQSEII
                     IAATTTAAYESYYGSSPDLSVKDNMLNAIDGQNTKGIGTAYLDNAAETLVLFYWDGSS
                     ATVKDVDYVLWGSLICAVDKSGVAGYQNDTPVAEQTCAPTHTDGFKLQRISDEGSEPS
                     SGGNGITGHNETGENLDGTWQVVAVGNTKPKLSSINYSPSSPLVGQAITFSVNVTDDG
                     SIAAVRLIYSFQSETGTVEMSLTTGNTYSVTIGPFSIAGILYYQIEAEDESGLISTSN
                     QNMITIQAPPEMLTIKTIRENWDEYNGQTVTLRGVVTIGSNILITSRTSAYFQDVSGR
                     GLNLFASAITNLMQGDSIEVTGELTEYNGVKELTNWTNSYTVLAQNVPITSTAKVYIS
                     ELANNLTDWEGSYVEIHGTVAERSNPTATNQGCNVVIEDETARTTVRIWNTTGALYNS
                     LGEIVNEYTDSLLTLGNQVIVRGVVGIYNNAPQILLGYASDVEPYIVGEVGEGRTKIS
                     VAPYPFVPKLGEVIKYTYEYPANCRAIVRVYDLSGRYITTLVDNYFAVSWKREDTWNG
                     RNELNQLVPPGNYILHLQTTNRSTGKSQISIAPVVVGVKF"
     gene            9989..10906
                     /locus_tag="PLI55_03590"
     CDS             9989..10906
                     /locus_tag="PLI55_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99954.1"
                     /translation="MRTFKHLLIAFLFIIIPIALWAQVFTQVNEIAAPISATGSSNLA
                     NIGSAWAQLNNPAGLCRLTALELVGGLYRPYGLSYYNSAVAIIAMPFAKVGTMAIGYQ
                     GSSTSYSGNSLTSETAFSLSHGFYLQKDLFSTLSLGYTLNLYQLDYGHSAGVSGDGSD
                     GMDLGSGFGFGLDLGIQGSLHERSWIGLFVKNINSPEMGSALSAQRLPRSVAIGLGYE
                     PYYGLTTNFVIYQKIGTDKTQYRAGLEYRIVPWLVLRTGVNTEPNRISFGFGIEKWGI
                     LADYAYISHPLLPETHQFSLGYSFRKNEK"
     gene            10896..13391
                     /locus_tag="PLI55_03595"
     CDS             10896..13391
                     /locus_tag="PLI55_03595"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPA99955.1"
                     /translation="MKSKFMRHSKYCWLLILFIMALNIIVGAVELESKIDLNTADEAE
                     LARLPLTRQQLEDILYWREYIGPFNSIFDLKNIKSIDHETFLKLKELTQISPVFLEES
                     EKRIEDNYYKVEQWISDDGASENTVNNWIDMLSNPVNINKLSYFELRNLAGLAPSDAV
                     AIVNRQKQGEIKNRTDLRSTEGLTYYGFSNLEDFIRYTDDDNVHRFGGSFSSVVKNIT
                     LSQTPSDDADSYAAFVQLDQPLDTYYKMRLHWGTKFRLEGSYLRNLGEQPIYYQGTKI
                     PQFKAFFEINNVEIGAVKLNKIVIGDYLAGFGQGVTFEATDFFMPRKTGYGFRNRLNG
                     LSGNSSRITQYGLRGIGAEAQIGNLIGTGFFSINNRDAVVNDDGSFSTLITMYPRMSH
                     GLYGNIPHNMINAVKEMVIGGNAKYSFVPGTYLGLTIYQSLYDRELDLHVTESLLDPS
                     GYSKYLTQIGNSADAEINASYESSAPSSIWSSAKSIRRVYGLEFSTVISNLTLQGEYA
                     ELDKDLKLGDRSGDPSALVVSGYLQFDNFNFLMLYRDYDLDFDNPYQRSYSNYQRFKG
                     TIYEDVYYLKDPSLAYLYSATAQPQAERGLYLSTRYQLHRSLIATAEQDIWTRVADNA
                     QYYRTVLNLEYRPVFKYRFKVRQKWQHRDKTNTLTPVFYQSNETRLEATMRMSRYNQV
                     KLLYSISFTEFTPRSRLVINAETGETSYVGNAGSPSDALGLTVTHNVNERLKLIGSVM
                     TYSGFLWNFEDTDFRVFNTDTRALHGWITVFSRLSRDLSLRLKYSFDLHNQLDNVVGG
                     MIDIGADSSNPVVSEVYYDKFYSDFRIQLDYRF"
     gene            13392..14738
                     /locus_tag="PLI55_03600"
     CDS             13392..14738
                     /locus_tag="PLI55_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99956.1"
                     /translation="MKKQIFILTFVLIVSSTGLSQSFFSPLIGDLPSVADARSLAVGL
                     SGWGDGGSASALLTNSALLGYSKQKLSLIAGLNAFSIKEKRSFPVQDSYGDFLADNTY
                     VLNQNWFPDFQVGINAKPLARIRLAMLLNHTNLSDFQYEEEVRGSLYGEYNRDPLVGY
                     NRLSNRYSVFNTSLGMAVNPWKSLWLGAALNFITPDFSEQKYEIQVIKPSDYQSSDHT
                     ISYVVKPKLESAITGNFGMAFDVTKHLSLAISYRLPYNTVQKGGLLYLVNDTTQTLPV
                     LAIDSVFTIEKVTYHQPGELRFGLCLKPVNIIPTQLFFEIVYQNWKDASAKYQFRSDA
                     NPNDIPNNFSDVPIPYRNVTKIHLGIEHIFFSGMPLRFGFYHDPNPINAEMNRNWFTA
                     GTAYQFGNFNLEISGAFTTCDYKYHDLFPIAVEKRVEFDTVRESYLIGMATLRYNF"
     gene            14742..16379
                     /locus_tag="PLI55_03605"
     CDS             14742..16379
                     /locus_tag="PLI55_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPA99957.1"
                     /translation="MKYKRSSIILIVVLIAETLLAAEKFPAKDYLKIVTIFTNDMHGG
                     IDRSEASFINPDFPPMLGGGAVAGRYILQKRQEAAQNGWGFLLIDAGDFYQGTPLGTV
                     SEGEAVIEYMNTVGYDALTVGNHDFDNGWKNLKKLAEKANFPFLATNLYRKSTGELAE
                     FVKPYIIKEIQGIKIGIIGATLSATPSMSYPEHIEDLDFRPEIEGIKRYLPELKAQGV
                     NTVIVLTHAWLAYDPEEGYKQMMADLSDGELGESYNTSAQAIAHRIPGIDIMFSGHLH
                     RGFYEPWEDPLNHTLIFQNYANGSNLGHVNFYIHRQTGTLAGYDYESDDGAIFTLFED
                     DFLPDTSLTRLIDFWVQKAEAGFDEVIGLATGQIYRSSSGESPMGNLMADAMLASTKA
                     DIAFSNFGGVRADLPAGQITPRHVFKVMPFDNRIVIMRVTGKFLKELVEDRVSGSNQG
                     MLVGGAEIVIDRNQPDGSRVIKFNIGGKPIEYEREYTLAVTDYLAEGNSGYDLLTTIT
                     PDRTNYTGILLRQAMIDYIREHSPIVPKTDGRWREIR"
     assembly_gap    16450..16549
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16550..18171
                     /locus_tag="PLI55_03610"
     CDS             <16550..18171
                     /locus_tag="PLI55_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPA99958.1"
                     /translation="PLDSEFINYIEMVKKGEVKKFTPEGYALGYIPHPLTFETEIPIH
                     LQKQVSLPERYDLREEGKLTSVKDQKDCGCCWTFATYGSIESRWKALELGDYDLSEQN
                     LRNGHGFLLGSCEGGNAKMATAYFSRGNGPIAEVDDPYNVKDSIYVSGLSSQAYVPDA
                     RFLPDDINILKQIVYTYGAVYTDMFWDNTYYNSQNHTYYSYVDSSINHAVLLVGWDDS
                     KETAGGTGAWIIRNSWGSGFGENGFFYISYNDTKVNTSPAFWPNRMDYNPNATVHYYD
                     KLGCVTSFGWVDTSLAHPDYEDYGLVKFVISTDQKITKVGTWVHSSNSTVNFYIYDNF
                     NGSSLSTLLGSTGDKICDYAGYYTFDLPDSIEVNSGDDIYIKVKYNTPETQYSLPAET
                     FITDYADPVIETGKCWISSTGNNNTWLAIGDNTDYKYDLCVKAYGVSTNTAIVGGDDI
                     IPEQILLYQNQPNPFNPVTTIRYSLSTSQYVVLKVYDVLGNEVATLVNEYKIPGNYAV
                     NFDATDLTSGIYLYQMKAGKNIQTRKLMVLK"
     gene            18435..19457
                     /gene="hydE"
                     /locus_tag="PLI55_03615"
     CDS             18435..19457
                     /gene="hydE"
                     /locus_tag="PLI55_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPA99959.1"
                     /translation="MQKTEIIEFLQGKDDNWLFNEADRIRRAVFGDEIYLRGIIEFTN
                     YCRQNCYYCGLRRSNRAVQRYRLSREEIIAAVETAVSEKIPTIVLQGGEDPYYSAEWI
                     ADLIRKIIDKYDLAITLSLGEQTPEVLQLWRKAGATRYLLRIETLNRENYGFLRPGKR
                     WEDRLNCLKVLRELDYEVGSGIMVGLPGETLATLADAIIGLTELDLDMIGIGPFLAHP
                     QTPLKDKPNGSIQLTLRTLALVRILNPYANMPSTSAMECAEKGARLAALKIGMNVIMP
                     SITPERVKLLYNIYPGKNADSDASEHSIPAVLEIIREAGFHRSLSKGISPNLIRRHES
                     QNILSK"
     gene            19481..21004
                     /gene="hydG"
                     /locus_tag="PLI55_03620"
     CDS             19481..21004
                     /gene="hydG"
                     /locus_tag="PLI55_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPA99960.1"
                     /translation="MQNVKVSEWTKKVIKPDEIEKYLIEGKDFIDETVITSQLKSAAN
                     PDKSRVREILAKSLEIERLEPEETATLLNVTDDDLWEEMYLAGLAVKRKVYDNRIVFF
                     APLYCSNLCVNNCAYCGFRYENKEEKRRILTMGKIRKETEAVINEGHKRMIVVFGEHP
                     RSAVDYIAETIRTIYDVKVPVKTGFSAIRRVNVNAAPMSIANLKKLWEVGIGTFQVFQ
                     ETYHKPTYANLHPLNTIKGNYRWRLYSLHRAMEAGIDDVAIGALFGLYDWRFEVMGLL
                     YHTIDLERHFGIGPHTISFPRLTPASGSWFSRNSQWRVNDADFKKLVTVLRLSVPYTG
                     LIVTARESAEMRREVIGVGCTQTDASTRIDIGGYTSALQALAKKEKADQQIDEQQFTL
                     GDTRSLDEVVRELAEQGLITSFCTAGYRCGRTGGKIMNLLKGCVEGKFCKANAVLTFR
                     EYLDDYASPETKTVGERLIQKEIEEIKAMPFFQKGRMLETFEKLYQQVASGERDVYI"
     gene            20991..21305
                     /locus_tag="PLI55_03625"
     CDS             20991..21305
                     /locus_tag="PLI55_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99961.1"
                     /translation="MSISKEILQSAASQTNLPDKLNAIAGKLEEEYGLQVNFCKIYGK
                     RWSYVAGDRNLEFAPQRRQLTSRYGIFYKQNDQINPDKMNEIFTALTQILTEIENDCR
                     TQ"
     gene            21350..22555
                     /gene="hydF"
                     /locus_tag="PLI55_03630"
     CDS             21350..22555
                     /gene="hydF"
                     /locus_tag="PLI55_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPA99962.1"
                     /translation="MQSTPKSNRLQIGIFGRRNVGKSSLMNALTSQYVSIVSDVAGTT
                     TDPVEKPMELPPLGPVLFIDTAGVDDEGSLGGQRIARTRKILERVDLGIIVAEGKNWN
                     KYEDDLLKELQNRHIPTVVAFNKSDLSQPSENLQAYLKQQNIPFINTIATDGFGVLDL
                     HKILIDTTPADYFNSIGIIGDLVKPGEMAVLVVPIDQEAPKGRLILPQVQTIRDLLDN
                     NAGCIVIKENELTSVLNNLKQPPAIVVTDSQAFLKVAAETPADIPLTSFSILFARYKG
                     DLAEYIAGTRTIDKLKPGDEILIAEACTHHPITDDIGRVKIPRWLNQYVGGQLNFTHY
                     QGHDFPEDLSKYKLVIQCGSCMLNRREVLSRIWYCKQAGVPVTNYGLTIAYTLGIFER
                     ATALFNIEA"
     gene            22690..23187
                     /locus_tag="PLI55_03635"
     CDS             22690..23187
                     /locus_tag="PLI55_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HPA99963.1"
                     /translation="MKNKWMSVILILLLASSLFSAGKEWYSFNEGIALAKKENKHIVL
                     DFYTDWCGWCKVMDQKTFSVPTVNKYLFENFVPIRINAENDKEKLVFQGKTYTPRQLT
                     RAFQVTGFPSIAFITPEMEVIEVIPGYIEKDLFLNLLKFVHQECYKTGISFDEFVKNG
                     CHGSK"
     gene            23543..24163
                     /locus_tag="PLI55_03640"
     CDS             23543..24163
                     /locus_tag="PLI55_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99964.1"
                     /translation="MKDSIQKNIANQINNRVIPRWEWRTFGSEFPKAEKFLSSLPYDT
                     IKESEEIYILSKNSNENCKIRNSLMDIKIIEQTNPDGLELWKPVLKASFPLSFEIVRL
                     ILRTLHVRKPLAMRDHYTHHQFLNEIVASNPKLSLVDVKKKRRCYTLEGAEVELSELQ
                     VGNQSVKTIAIESEKPQNVQKVLKLLELDNLPNTNYIKALKKMNRH"
     gene            24165..24638
                     /locus_tag="PLI55_03645"
     CDS             24165..24638
                     /locus_tag="PLI55_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016474186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPA99965.1"
                     /translation="MGIEIERKFLVIANDYRTSGNGVRYRQGYLSTAKERVVRVRTVG
                     QQAFLTIKGINVGAVRPEFEYEIPRQDAEQILENICEKPILDKIRYRVKHSGFIWEVD
                     EFLGDNAGLVIAEIELADENQSFIKPDWVGAEVTGDPRYFNSNLIKNPYKSWNIK"
     gene            24755..24841
                     /locus_tag="PLI55_03650"
     tRNA            24755..24841
                     /locus_tag="PLI55_03650"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24789..24791,aa:Ser,seq:tga)
BASE COUNT         7043 a         4912 c         5513 g         7278 t
ORIGIN      
        1 attgggttac tgttttttgg gaaaatcttt agcatatttg tcgagtttat gatagaaatt
       61 acgacgatca atgccataaa tgcgggcggc tttggtaata ttgccatggg ctttctcgta
      121 tgcattttta attaattcct tttcaaattc ccgggtaaat tcagcgtaag ccgttcgata
      181 atttgggtag cgatttatat cgtttttata taccgtaaag aagctttgct ccggctcttc
      241 cgaatcaaat tcagagggaa atgaaatgtg ggagggcgtg atcgtatcgg tagcgagcag
      301 ggcggcttta cgaataacat tacgcagttg gcgaacattg ccaggccaat tatatgaaag
      361 taatttagcc atcgcctctt cggaaatatc agtgatttta ctgtttaatt caaaattagc
      421 ttctgataaa aataacgcag ttagatgggg gatatcctca agtctttcgc ggaggggagg
      481 catcgtgata gtatattccg agattcgata atataaatct ttacggaatt tattttcact
      541 gaccagctga agcagattgg cattgctggc gatgattagg cgaatatcaa ctttgctttc
      601 attgatggca ccgatcgggc gaaaggtctt gttttcgatg acacggagaa acggccgttg
      661 ttgtgcaagg ggtagattgc cgatttcatc gagataaaga gtaccttgat gagccagttg
      721 aaatttcccc gggcgacttt gggtggcacc ggtgaacgcc cctttctcga aaccgaaaaa
      781 ttcgctttcg attaaatttt ctggtatggc gccacagtca atcgcgataa atggttgttt
      841 ggcgcgcttg cttttattgt gtaatagatg ggcggcgacc tctttaccgg ttccggtttc
      901 accttgaatc aggacagtta agtcggtctg agcaacggaa ttgatctgac tgataacttt
      961 tttaattgcc ggactatgac cgagaacctg ttcgctgtct tgatcggttc ccaacatctg
     1021 gcgtaagtgg gcaagttctc gcttgacttg ttgattttcc aagccgcgtt ttacaaccat
     1081 taataaaacc gcgttctcga acggtttttc gagataatcg taagccccca ttttcatagc
     1141 attgacagcc attgagactt cgccgtaagc agtgatgata ataacgacca gaccactttc
     1201 gatttcattc tggatctgtt tcagaatttc gtgtccggtt gtatctggca ggcgtacgtc
     1261 caggattacg caatctggtg tttgggtttt aattatccga agagcggttt gaccattatc
     1321 ggtagttcga actttatagc cgttcatagt taaaagttcg gcaatgtttt cgcgagtgtc
     1381 ttgagaatct tcgactatta atatagaatt cataataaca tcctgatttt aagtgtctta
     1441 aaacatatta ctggaaggct ggaatagtta ctttaaaagt ggtgccgtgg tgtaaaatac
     1501 tatttacgga aattttacca tggtgaaatt cgataatttc cttggtgagt gccagaccca
     1561 gtccgaaacc tttggacttg gagctgtaaa atgactgaaa aatctgcggc aagtcttcag
     1621 ctggtattcc ggagccggta tcggtaaaca gaaattgaac ctgtttttcg gtttttaagg
     1681 ctttgacgtt gagagtgaat cgtcctttct tagtcattgc ttcgaacgca ttattgatta
     1741 tgttctgaaa agcctgagtt aatcggaatt cgtcaatata gatgggcggt aagtctcggt
     1801 cgtaattcac agaaattttt atatatgggt attcgtttaa tttttcttcg ataaaatcgt
     1861 ttatggactt gatgaattta gcggtattta cttcttcaaa ttggtaattt gtggcatgcg
     1921 cataggagaa caggtcccga atgactttat tacaagtatc aacgttgcga ttgataatat
     1981 ccatatgttt ttcaacctga ggattatgat tgttattcat actggcaact aactgagcag
     2041 aacttttgat gcttgccagc ggatttttga cttcatgagc tagtttcaag ataaattgac
     2101 gcatactttc gcgcttttgt tcttcggatt cacgaagttc ttgctgggcg tgtcgtattt
     2161 cggtaatatc ccgaccaatt ccatgaattc cgatgatctg gttatttttc attataggac
     2221 gcatttttaa ccagatgggg cgaatttcac cggatttggt cataacattt agttctgtac
     2281 cagttggacc atctgtaaga actttcaaat aacaccgaat tatgcggaat tgatgttcgg
     2341 gatggattaa agttaaggga ttgatggcaa acaattcttt tttagagtag ccggataagt
     2401 ttttaaagtg gttgttaacc gatagaaatt taccggtttt atccatagtc cagatgagat
     2461 cattggcgtt ttggaatagg tcgcggtatt tttcttcact ggcgcggagt ttgttttcga
     2521 tttttttacg cgtattgatt tctttaagga gttgtcggtt ggtttttctt aattccgcgg
     2581 tgcgctgatt gacttgtttt tcgagttcag atcgagcatt gcgcaggaaa ttttccattt
     2641 ccctctgatc ggtaatatcg cggatataga tggcgatgtt gttgaccttc tggtccattc
     2701 caataatagg ataaaaatga tagtgaaaaa ttcgtccccg gttttcgtcc tcaaattcct
     2761 gaggcgattg ggtactgata catttatcga ggaatatttt tcggcgctcg aataaattta
     2821 gaggtgagaa ctcacttaga ttatgattga tgacctcgtt ctccgaggct tgaaaatgtt
     2881 tcaaaaccga attgttagcg gctaagatat taccgtcagg gtcaattaac attgccagat
     2941 caataggagc attgagcaga acgcgagcga cttcttcact gcgttttaag gattcttcgg
     3001 ctttcatccg aatattgatt tcgcggttta attccacatt ggcttcggcg agttcctggg
     3061 ttctttcctg gactttttct tcaagttggt tatgagccgc ctcaagggct ttggattttt
     3121 caatcaaggc ttgagtgcga tcaaccacct gtttttctag ttcttccttc tgatggcgga
     3181 taaccgcgat gcgtatgtag tttatcaaaa caacaacacc taaaccaaga acccatatta
     3241 gtattttgaa ccacgctgtt ttccagaaag gtggaacaat tttaatcctg atggtggctt
     3301 cattgcgact taaaacgcca tccgaatttt cgcctttgac tcggaaagta tattgaccgg
     3361 gtgggacttt agaatagcta acgctattag ttttaacgca ttttttccaa tctttatcat
     3421 atccttctaa aatgtagtag tatttatttt cagcggggat atagtagtcc agtaaactaa
     3481 aactgatggt gaagtaattc tcattccaag gtaggtctaa tgtgatttgg ttaggagaat
     3541 gattggaaat tattttttca gtatagtcat ttttaaattt cgtaataacg atattgggca
     3601 catatttatt attaatacta tccggatgaa aataagtata gccacattta ccgccaatca
     3661 taatccagcc ggaacgcaat cggcagtaat tccttaagat attatttttt aggtagctgg
     3721 gttctctgat gtcagaggtt gatctgtctt tgtaatagta atttacattg tcattgcgat
     3781 cgaagagggt aaaagtattc aatattggat tgtgttttat gattccttga gtggttctaa
     3841 accagaaaat gccattatga tcttcaatta cgccggtaat tgggtatttc cagatatatc
     3901 ccgaattttc ggtacgatag acaaatttct ttgtcagcgg attgtatgaa atcaggtatg
     3961 gtaacgacga ggtcaaccaa atctgaccat cttgagcttc gtataaacgg taaaaacctt
     4021 cgtcactttc cggcataaaa atcaaagtac tgtcagaaat taattgataa gtaccgctat
     4081 ttcggtcaaa taaaatgagt ccgttgcgag tcgccaacca taaattcttg ttccgatctt
     4141 ccagaatgtc gtaaacgata tttttgttcg ggacgttctt agtcggtcca gaaattgagt
     4201 aatattttct ttgattggtt tggcgatcgt agcggcataa accagcggcg gtacccagcc
     4261 aaaaattacc ctcgtgatct tcatagatag ccggtatatg gttggctcta atcgaatttt
     4321 gcttattcgg ctgattttta gtgacgtgat ttaaatagat aaattcttga gaagatggat
     4381 tgagtttgaa taatccaaga gtagtaccca cccagattgt attttgagag tcttcataaa
     4441 tatgtaatgt gcccggtggt gggctttctt gattggagag ctttaggggg aaaattttga
     4501 cattcttagt tttcggattg tagcgcagaa cctttccttt tagaccaaac cagaggttat
     4561 tttcgtgatc ttcaagaaca tcgttcacat tgtcgttatt aaaattaatg ctgtccgata
     4621 ttgaacgtaa atccataaaa gggcgtggta aagaaaatct aaacacacca ctatattgag
     4681 tagcgagcca ggccgaaccg cttttatcaa tgaaaaaaca gttaatatta ttaaccgata
     4741 tttgattttc atcataaggg tcggtcgaat aatgccagaa tatttctgtt tccggatcat
     4801 atctaaagaa ggtctcgttc tgattgcatt ggcttccaac tacccatatt tgtccgccac
     4861 ttccctcata taattgattg atggtatagt aatagtaatt ttttatcgga tcctggtagg
     4921 gatttatttc actgccgtcc ggtttgagcg gcagtagata atattgcaat gagccgtcgg
     4981 caatggaata ttttgccaga aatttatggg cggctatcca caggttttga ttacaatcga
     5041 gtaatatatc atcaatttta aaagagaaac ggttgaagta ataattaaga ttctggaagt
     5101 cgtcctcgtt aatcctgcca gtatttatat cgtattcgac tatgccgtat tgacttaaac
     5161 ctaacagtaa ttttccatcg cctttattta ttattcgatg aattacataa ttggcaggca
     5221 atatagaatt atcgtcaaac actaaaggac tgattttttt tatctggtat gaaccatttt
     5281 gattaaaggc tttcaaaatt tggttatttt gttgtctgat atcggtgacc gagaaataga
     5341 aattacccaa acgatcttcg gctatatcaa taatgctggg aatcgaatcg ttcggcgata
     5401 ggatatgaat gaaaccgttg gtaagcggat cgtatttatc gatacaacct ttatgaggga
     5461 tgtcgcccac tgaaccgata atccagagcg caccttttga atcaataaat aatcgcttaa
     5521 tattttgaca ggtgagagaa gtggctactg aatcttcggg atgataagta acaatctcgt
     5581 tcccatcgta gcggcacaat ccattttctc tggtggcaaa ccacataaac ccaattgaat
     5641 cctgaacaat ggaggtgata tggttactgg gtagtccttt ttcagtgtta atcttctcga
     5701 aatgcaagcg tttgccgggt ttgatgaccg ttttagcaat tgatatttgg gcatttgtga
     5761 ttaataaaca agtagatata tttagaatca ataacctcga aaagcggccg cctcttagga
     5821 cggctggttt ctcgttatta atttccggtt ttttaaaaat acttctcatc gcgttaagcc
     5881 gaattatctt ccaaccagct taatttaaaa ataattttga ggatgtaaag atagaatgta
     5941 actttctatg tgatttttat tcaaattaga gtggctaaaa atcagaaaac caaccagcaa
     6001 aattatttaa aaactcttat tagccaacgt tcaaaaggta tttaaaaaat tagagaggtt
     6061 attatgcatc aaaccatcaa aattttcctg atcgctttga ctttgccaac cctgattttg
     6121 gctggtgatc tgttcttcag cgaatatatt gaaggatcta gctataataa agctattgag
     6181 atatacaatc ctacaggggc tgaagttgat cttagcgatt atactattaa acaatctaac
     6241 ggtggttcag gctggggatg ttatacgact gatgatggag taataacgga agatacgcgc
     6301 tatatacttc cattaagcgg tactctaagt cctgacgatg tttatgttat ttatcataat
     6361 gatgccgctt cagagattaa aagtgttggt gatattggat tttcatatgt tcaaactccg
     6421 aatggtggaa atggagacaa tgtaattaat ttcaatggta acgatgctct gggattattt
     6481 aaagatggtg tattgattga tgtcattggt gtacctacag aaaaccctgg tgaagataaa
     6541 ggctggacag tcgccggtac ttctaatgct actaaagacc acaccttggt tcgtaaatct
     6601 actgtatccc aaggtaatac cgattggacc tcttcagccg gtacttcggc cgatgattcg
     6661 gaatgggaag ttcatcctca gaatacattt acttatttag gttcgcacgt ctggagtggc
     6721 ggtggtaatg tttctcctat ggcannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnttcacc cgattttatc acttcggcgg acgagattac cgtcagcgca acgattacgc
     6901 cggtggtagg gagtatttct tcggcgaaga tctattacgg agcacccggt agtttgatca
     6961 acaccgcgga gatgtggaag gataccggtg atacttggat gggcaatata ccgccccaga
     7021 ccggcaatag cattatcgaa ttttatattt attgcgagga cgatcagact ccagccaatt
     7081 ccggtaaatc agctacccaa acgctgataa tcgccaatag cactcccgag acaatcgcca
     7141 atattcatac aaacattgag actttaaatg gcaaaatggt cacgattcga ggaattgtca
     7201 cgatcggcgc cggtgtttta gcaagtggtt atacttctgc ctatattcag gatgcgtctg
     7261 gtcgtgggtt gaatctttat gagtatggca aattatattc tgatattacg cgcggtgatg
     7321 aattgatggt agtcggttat gttgagaaat ataacacgac cattgaagtt actaatttta
     7381 tctataagaa aatctccagt ggcaacgaat tgccaccggc tcaggtcgta acggtcgccg
     7441 gtgctaatag cagtgactgg gaaggcacac tgatcgagtt ttccggaatg gtaaccaaac
     7501 aggaaacttc cggtgaggcg accaatatta gcgtttcggc tgggactgat acgactatag
     7561 cccggattcc ggatgctacc ggtattgatg ttgcatctat cgcactgggc aattcttata
     7621 aattcagagg agtcggcaat aaatataaaa gtacttatca aaccctgatt ggttacgccg
     7681 aagatattgt tccattaacg gcggttaaag aaagcaagac caatgctctg actttcaaac
     7741 tcaacccggc ttatcccaat ccgttcaatc cggcgaccac gattagttgg cagttggata
     7801 aaaatggcgc ttacgaagtt gtcgtgttca atgttcttgg ccagaaaatt gacgtaattg
     7861 ccagcggtta tgccaccgcc ggaagttatg cgaaagtctg gaatgccgcc aagtttccat
     7921 ccggcgtgta ttttgttcaa ctgcaagccg gaaaccgcgt aagaacgcaa aaaatgttgt
     7981 acttgaagta acctatgaaa aaacgaatta ttctacctgg gctgattcta ttgccggttt
     8041 tactattaac ggcgcaggat cacgtcgtca tttccgagat tgtcctccag ccgagtaatg
     8101 ccgaatacat caaactttac aatccgacgg ataatgcagt tgacctgagt aattattatc
     8161 ttaccgacgg cactgatcca tcaaaccaaa aatattacca taatctacca accggtacca
     8221 atttctggag tggaagcggt tcggatttca tagtacgttt tccgccgggt tatagcattg
     8281 aggcgcaatc agaaatcatt atcgcggcta cgaccaccgc ggcttatgaa agttattacg
     8341 gctcttcgcc cgacctgtcg gttaaagaca atatgctaaa tgcaatcgac gggcagaata
     8401 cgaaaggtat cggtacagct tatttagata atgcagcgga aacgctggta ctgttttatt
     8461 gggatggctc atcggcgacg gtcaaagatg ttgattatgt cctttggggc agtctaatct
     8521 gcgccgttga taaaagcgga gttgctggtt accagaacga tacgccggtt gccgaacaaa
     8581 cctgcgcgcc gacgcatacc gacggcttta agctccagcg cattagtgat gaaggctcag
     8641 agccatcatc aggcggcaac ggcattaccg gtcataacga aaccggcgaa aacctcgacg
     8701 gcacctggca ggtggttgcc gtcggtaata ccaaaccgaa attatccagt atcaattatt
     8761 cgccgagtag tccgcttgtc ggtcaggcta taactttttc ggtgaatgtt accgacgatg
     8821 gatcaatcgc ggcggttaga ttgatttatt cctttcaatc ggagactggt accgtggaaa
     8881 tgtcgctgac taccggaaat acttattctg ttaccattgg tccattttca attgccggta
     8941 ttctttatta tcagattgaa gccgaagatg aatctggtct aatcagcact tctaatcaga
     9001 atatgattac aattcaagcg ccaccggaaa tgctgactat caaaaccatc cgcgaaaatt
     9061 gggacgaata taacggtcaa accgtaactc tgcgaggtgt ggtgacaatc gggtcgaata
     9121 ttcttattac cagccgaacc tcggcttatt ttcaggatgt ttcgggaaga ggattgaacc
     9181 tctttgcttc cgcaattacc aacctgatgc agggcgattc catcgaagtg accggcgaac
     9241 tgaccgaata caatggagtc aaagaattaa ccaactggac taatagctat accgttctgg
     9301 ctcaaaacgt cccgataacg agtactgcca aagtatatat ttcggaattg gcgaataatt
     9361 tgactgattg ggaaggcagt tatgtcgaaa ttcacggcac tgtagcggaa cgcagtaatc
     9421 cgaccgccac taatcaggga tgtaacgttg ttattgaaga tgaaactgcc cgaacgactg
     9481 tgcgcatctg gaatacgact ggagcgcttt ataattcgct gggcgaaatc gtcaatgaat
     9541 ataccgattc attattgacc ctcggcaatc aggtaattgt gcgcggggtt gtcggtattt
     9601 ataataatgc accgcaaatc ctgctgggtt acgcctcgga tgttgagcct tacattgttg
     9661 gtgaagttgg agaaggacgc accaaaatca gtgtcgcacc ttatcctttt gtaccgaaat
     9721 taggcgaagt catcaagtac acctatgaat atccggcgaa ttgccgcgcc attgtgcgag
     9781 tttatgacct gtcaggtcgg tacatcacga cgctggtgga caactatttt gccgtttcct
     9841 ggaaaaggga agatacctgg aacgggcgca atgaattgaa tcaattggtg ccgcccggca
     9901 attatatttt gcatctacaa acgaccaatc gttcgacggg aaaatcccag atttcaattg
     9961 cgccggtagt ggtaggagtg aaattctgat gagaactttt aagcacctgt taattgcttt
    10021 tttatttatt ataattccga tcgcactctg ggctcaggtt ttcacccagg tgaacgaaat
    10081 cgccgcccca atcagtgcca ccggttcatc caatttagca aatatcggtt cagcctgggc
    10141 acaactcaac aatccagccg gtttgtgtcg tttaaccgct ctcgaattag tcggcggttt
    10201 atacagacct tatggacttt cttattataa tagtgccgtg gcaattatcg ccatgccatt
    10261 cgccaaagtc ggcacaatgg cgattggcta tcaaggcagt tcgacctctt attccggcaa
    10321 ttccttgacg agtgaaacgg cttttagcct gtcgcacggt ttttacctgc aaaaagacct
    10381 attttcgacg ctatcgctcg gctatactct caatctctac caacttgatt atggtcattc
    10441 agcgggcgtt tccggcgacg ggagcgacgg gatggatttg ggaagcgggt ttggttttgg
    10501 acttgatctg ggcatccagg gtagcctgca tgaaagaagc tggatcggat tatttgttaa
    10561 gaatatcaat tcgccggaaa tggggagtgc cttgagcgct cagcgccttc ccaggagtgt
    10621 ggcaattggt ttaggctatg aaccttatta cggattgacg accaattttg tgatttacca
    10681 gaaaatcgga acggataaga ctcagtatcg tgccggattg gaataccgta ttgtgccgtg
    10741 gttagtcctg cgaaccggcg tcaataccga accgaaccgc atcagctttg gcttcggaat
    10801 cgagaaatgg ggcattctgg cagattacgc ttatatcagt cacccgctat tacccgaaac
    10861 tcatcaattc agtctgggct attcattcag gaaaaatgaa aagtaaattt atgaggcatt
    10921 caaagtattg ttggctattg atcctattta taatggctct gaacattatt gttggtgctg
    10981 tcgaacttga atcgaaaatt gacctcaata cggctgatga agctgaactg gcgcggttac
    11041 cgttaactcg ccagcagttg gaagatattt tatactggcg ggaatatatt ggcccattta
    11101 atagtatatt cgacttaaag aatatcaaaa gtattgacca tgaaactttt ttgaaactga
    11161 aagaactaac ccaaatctcg ccggtttttc tggaagaatc ggaaaaacgc atcgaagata
    11221 attattacaa agtcgagcaa tggatttccg atgacggtgc cagtgagaat accgtcaata
    11281 actggattga tatgctctcc aacccagtta atatcaacaa acttagctat tttgaattgc
    11341 ggaatctggc gggactagct ccaagcgacg ccgtggcgat cgttaaccgc caaaaacagg
    11401 gcgagattaa aaaccgcacc gatttacgca gtaccgaggg gttgacctat tacggttttt
    11461 ccaatttgga agattttatt cgctatactg atgacgataa tgttcacagg tttggcggca
    11521 gtttttcgag cgtggtcaag aatattaccc tcagtcagac tcccagcgat gacgccgatt
    11581 cgtatgccgc tttcgtccag ctggatcagc cgctggacac ttattacaaa atgcgtttgc
    11641 actggggcac gaagttccgt ctcgaaggtt cttacttgcg caatttggga gagcaaccga
    11701 tttattatca gggtaccaaa attccgcaat tcaaagcctt tttcgaaata aataacgttg
    11761 aaatcggcgc ggtaaaattg aacaaaatcg taatcggtga ttatttggct ggttttgggc
    11821 aaggggtgac ttttgaagct accgactttt ttatgccgcg caaaaccgga tacggttttc
    11881 gcaatcggct gaacggttta agtggcaatt cttcgcgcat tacgcaatac ggcttgcgtg
    11941 gtattggtgc ggaagcccaa atcggcaatc tgattggcac cggatttttc tccatcaata
    12001 atcgagacgc agttgtcaat gatgatggtt cgttttcgac tctaattacg atgtatccgc
    12061 ggatgtcaca cggtttgtac ggcaatattc cgcataatat gatcaatgcc gtcaaagaaa
    12121 tggtgatcgg cggcaacgcc aaatattctt ttgtgccggg aacgtattta ggtttgacaa
    12181 tttaccagag tttgtatgac cgggaattag atttgcatgt taccgagtca ctgcttgacc
    12241 cttccggcta tagcaaatat ttaacccaaa tcggcaattc cgccgatgcg gaaattaatg
    12301 cctcttacga gtcatcggct ccatcttcaa tctggtcatc tgccaaatct atccggcgag
    12361 tttacggact ggaattttca acggtcatta gtaatttgac cctgcaaggt gaatatgccg
    12421 aactggataa agacttgaag ttaggcgatc gttccggcga tccgagtgcg ctggtggtgt
    12481 ctggttattt acagtttgat aattttaatt tccttatgct ctatcgggat tacgacctgg
    12541 acttcgataa tccttaccag cgcagttata gcaattatca gcgcttcaag ggtacgattt
    12601 acgaggatgt atattatctc aaagacccca gcctcgccta tctttattcg gctacggctc
    12661 agcctcaggc agaacgcgga ttgtatctat caactcgcta tcaattgcac cgcagcttaa
    12721 tcgccaccgc cgaacaggat atttggactc gtgtagccga taacgcccaa tattaccgca
    12781 ccgtgcttaa tctggaatat cgtccggttt tcaagtatcg gttcaaggtt cgccagaaat
    12841 ggcaacatcg ggataagacc aatactctca cgccggtttt ctatcagtct aatgaaactc
    12901 gcctcgaagc cactatgcgg atgtcgcgtt ataaccaggt aaaactgtta tattcaattt
    12961 cgtttaccga atttacccca cgctcacgac tggttattaa tgccgaaacc ggtgaaactt
    13021 cttatgtcgg caatgccggt tcgcccagtg atgcgcttgg tctgaccgtt actcataatg
    13081 tcaatgaacg actcaaactg attggttcgg tgatgaccta tagcggcttt ctctggaatt
    13141 ttgaagacac ggattttcga gtgttcaata ccgacacgcg cgcgcttcat ggctggatta
    13201 cagttttctc acggctgtcg cgcgatcttt cgcttcgcct gaaatacagt ttcgatctgc
    13261 ataaccagtt agataatgta gtgggtggaa tgattgatat tggcgcggac agttccaatc
    13321 cagtcgtcag cgaagtatat tatgataaat tttattcgga tttcagaatc caacttgatt
    13381 ataggtttta aatgaaaaag caaatattca ttctgacatt tgtgttaatc gttagctcaa
    13441 ctggcttaag ccagagtttc tttagcccgt tgattggcga tttaccgtcg gtagccgacg
    13501 cgcggtcgtt agcggtcggc ttatcaggct ggggtgatgg gggaagcgct tcggctttac
    13561 tcacaaattc ggctttgctg ggatacagca aacaaaaact gtcgctaatc gcgggattaa
    13621 atgcattttc cattaaagaa aaacgcagtt tcccggtgca ggatagttat ggcgattttt
    13681 tggctgacaa tacttacgtg ttgaaccaga actggtttcc agattttcag gttggaatta
    13741 acgccaaacc tctggcgcgc atccggttgg caatgctgtt aaatcatacc aatctgagtg
    13801 atttccagta tgaagaggaa gtcagaggct cgttgtacgg tgaatacaat cgcgatccgc
    13861 tggtgggtta taaccgacta tcgaaccgct attcagtttt caatacttcg ttgggaatgg
    13921 cagtaaatcc atggaaatca ctctggttag gagcggcgtt aaattttatt accccggatt
    13981 tttccgagca aaaatatgag attcaggtca tcaagcccag cgactaccaa tcttccgatc
    14041 acacgatttc ctacgtggtc aaaccgaaac tcgagtcggc gattaccggc aatttcggga
    14101 tggcatttga cgttaccaag catctcagcc ttgcgatttc ttaccgatta ccatacaaca
    14161 cagtccagaa aggcggttta ctttatttag taaatgacac tacccagact ctgccggtac
    14221 tggcgattga ttcggttttt actatcgaaa aagttaccta tcatcaaccg ggtgaattgc
    14281 ggtttggatt atgtttaaaa ccagtaaata taattccgac acaattgttt ttcgaaatcg
    14341 tctatcagaa ttggaaagat gcctcggcta aatatcagtt taggagtgac gccaatccga
    14401 atgatattcc gaataatttt tcggacgtgc cgattcctta tcgcaatgtg acgaaaatcc
    14461 atcttggcat cgaacatatt ttcttttccg gtatgccttt gcggttcgga ttttatcacg
    14521 atcctaatcc gatcaatgcc gagatgaatc gcaattggtt cacggcgggc accgcttatc
    14581 aatttggtaa tttcaatctc gaaatttccg gagcttttac aacttgcgat tacaagtatc
    14641 acgacctttt tccaattgcg gtagaaaagc gggtcgaatt tgataccgtt cgcgaaagtt
    14701 atctgattgg aatggcaacc ttgaggtaca atttttaatc tatgaaatac aaaagatcat
    14761 ctattatttt aattgtcgtt ctaatagcag agacgctttt ggcggctgaa aaatttcccg
    14821 ctaaagatta cttgaaaatc gtaacgattt ttaccaatga tatgcacggt ggaattgatc
    14881 ggagcgaagc cagttttatt aatcccgatt ttccgccgat gctgggtggc ggagcagtcg
    14941 cgggacgtta tattttacaa aagcgtcagg aagccgccca aaatggctgg ggattcttat
    15001 taattgatgc cggagatttt tatcagggga cgccgctggg aacggtttcg gagggcgaag
    15061 ccgttatcga atatatgaat accgttggat atgatgccct gactgtcggg aatcacgatt
    15121 tcgataatgg ctggaaaaat ctgaaaaaat tggcggagaa agccaatttt ccatttctag
    15181 cgactaacct ttaccgcaaa tcaaccggag aattggctga atttgttaag ccgtatatca
    15241 tcaaagaaat tcagggaata aaaatcggca tcatcggagc gacgctttct gcgacgccca
    15301 gtatgagtta tccggaacac attgaagacc tggatttccg ccctgagatt gaaggaatca
    15361 aacgctattt accggaatta aaggcgcaag gtgttaatac ggttatcgtt ctgacgcacg
    15421 cctggttagc ctacgatccg gaagaaggct ataagcaaat gatggctgat ctcagtgacg
    15481 gagaactggg tgaaagttat aataccagcg cgcaggctat tgctcatcgg attccgggga
    15541 ttgatattat gttttccggt catttgcatc gcggctttta cgagccgtgg gaagatccgc
    15601 ttaatcatac cctgattttc caaaattatg ccaatggcag taatctggga catgtgaatt
    15661 tttatattca tcgccagacc ggaactctgg cgggttacga ttatgaatct gacgacggcg
    15721 cgatattcac cttgtttgaa gatgactttt tgccggatac cagtctgaca cgactgattg
    15781 atttctgggt ccaaaaagcc gaagccggat tcgacgaggt catcggttta gcgaccggac
    15841 aaatctatcg ctcgagtagc ggcgaatcac cgatgggcaa cctgatggct gacgccatgc
    15901 ttgccagtac aaaagccgac attgcttttt ctaattttgg cggagtcaga gccgacctac
    15961 cggctgggca aattactcct cggcatgttt tcaaagtaat gccgtttgac aatcgcatcg
    16021 tcataatgcg agtaaccggc aaatttctga aagaattagt cgaagatcgc gtttcaggta
    16081 gcaatcaagg tatgcttgtg ggcggggctg aaatcgtaat agatcgcaat caacccgatg
    16141 gttcacgagt tataaaattc aacattggcg gaaaacctat cgagtatgaa cgcgaatata
    16201 ctctggctgt tacggattat ttggcggaag gtaattctgg ttatgaccta ttgacgacca
    16261 tcacgccaga tcgcaccaat tacaccggca ttcttctgcg gcaagcgatg attgattata
    16321 ttagagaaca ctctccaatc gttccgaaaa ctgatgggcg atggcgggag attaggtaag
    16381 attaaggtcg aggttaatgt ttaaaactac tataaaattt attttgactt tatcactgct
    16441 cgctttttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tccgcttgac
    16561 tcggagttta tcaattatat agaaatggta aaaaagggtg aggtaaagaa atttacgccc
    16621 gaaggatacg cccttggcta tattccgcat ccattgactt ttgaaacaga aattccgata
    16681 catcttcaga agcaagtttc gttaccggaa aggtacgatt tgcgagagga aggcaagcta
    16741 acctcggtaa aagatcagaa agattgcggt tgttgctgga catttgcgac ttacggttcg
    16801 atcgaatcaa gatggaaagc tttagaattg ggggattatg acctttctga acaaaacctt
    16861 agaaatggcc atggctttct tttaggttcc tgcgaaggcg gtaatgccaa aatggcaact
    16921 gcctatttca gtcgcggaaa tggcccaatt gccgaagttg atgaccctta caatgtaaaa
    16981 gatagtattt atgtttcggg tttaagttct caagcctatg ttcccgacgc tcgattttta
    17041 ccggatgaca taaatatcct taaacagatt gtatatactt atggcgctgt atataccgat
    17101 atgttctggg ataatactta ttataattca caaaatcata cctattatag ttatgtggat
    17161 agtagtatca accatgcagt cctgctggta ggatgggatg acagtaagga gactgctggt
    17221 ggcacaggag cctggattat aagaaatagc tggggttcag gtttcgggga aaatggcttt
    17281 ttctacattt cctataacga taccaaagta aatacgagcc cggctttctg gccaaatcgt
    17341 atggattaca atccaaacgc tactgttcat tattacgaca aattaggatg tgttacatcg
    17401 tttggttggg ttgatacttc tcttgcacat cccgattatg aggattatgg tctggtaaaa
    17461 tttgttatat ctactgacca gaaaattacc aaagtaggga cttgggtgca ttcttccaat
    17521 tcgacggtga atttttatat ctatgataat tttaatggga gctctctttc gactcttttg
    17581 ggttcaaccg gagataaaat ctgtgattac gccggttact atacctttga tttgcccgat
    17641 tcgatagaag taaacagtgg tgatgatatt tacataaaag taaaatacaa tactcctgag
    17701 acccaatatt ctcttccggc tgagacgttt attaccgatt atgctgaccc agttatcgaa
    17761 acaggtaaat gctggattag tagtacaggg aataataata cctggctcgc aataggagat
    17821 aataccgatt ataaatatga tctctgcgtt aaagcttatg gtgtttcaac taatacagcg
    17881 attgtgggtg gagatgatat aataccagag caaatccttc tttatcagaa ccaaccgaat
    17941 ccttttaatc cagtaacaac aatccgttat tcattatcaa cctcgcaata tgtcgtattg
    18001 aaagtttatg atgtccttgg caatgaagtg gctactttgg ttaatgaata taaaatacct
    18061 ggcaattatg cagtaaactt cgatgcaact gatttaacca gtggcattta tctttatcaa
    18121 atgaaagcgg gaaaaaatat ccaaactcgc aaacttatgg tacttaaata gtatcaagtg
    18181 acattaagat aggtagtatt ttctcttgtg aattaaaagc tggtttattt ttaccattag
    18241 attaacctga attattctaa ataatttttt ataggattaa cattattttt gttaaaattg
    18301 tgaactaaag gttatttgag tgatgatgta aaaagtaaga ttatgaaact attattgtgg
    18361 ataaagatat caaaagtgtg aaatctaaaa tttttatttg gctatttcta attgtcaatg
    18421 gttactaaat aactatgcaa aaaaccgaaa tcatagaatt tttgcaaggt aaggatgaca
    18481 attggttatt taatgaagcc gatcgcattc ggcgggcggt tttcggcgat gaaatttacc
    18541 tacgtggtat tatcgagttt actaattact gtcggcagaa ttgctattac tgcggattgc
    18601 gccgttccaa tcgcgccgtc cagcgctatc ggttatcgcg tgaggaaatt atagccgcag
    18661 tggaaactgc cgttagtgaa aaaattccga cgatcgttct gcagggtggg gaagacccct
    18721 actattcggc ggaatggatt gccgatttaa tccgcaaaat tattgataaa tatgatctgg
    18781 cgattaccct ttcactcggc gaacagactc cggaagtttt acaattatgg cgaaaggcgg
    18841 gcgcgacccg gtatttgttg cgcatcgaga ccttgaatcg ggaaaattat ggctttttac
    18901 ggccaggcaa acgatgggaa gaccgcctga attgcctgaa agttttacga gaactggatt
    18961 acgaagtcgg tagtggaatt atggtgggat tgcccggtga aactttggca acgctggctg
    19021 atgccattat cggattgact gaacttgatc ttgatatgat cgggattggt ccgtttctgg
    19081 ctcatcctca gacacctttg aaagataagc cgaatggttc aatccaattg actctgcgaa
    19141 cgctggcgct ggtgcgcatt ctgaacccct atgccaatat gccttcgacg agtgctatgg
    19201 aatgtgccga aaaaggcgct cgtcttgcgg ctctaaaaat tggaatgaat gtcattatgc
    19261 cgtcaataac tccggagaga gtcaaattgc tctacaacat ttatcccggc aaaaatgccg
    19321 atagcgatgc ttctgaacat tcgataccag ccgtattgga aattatccgt gaggcgggat
    19381 ttcaccgcag tcttagcaag gggatttcac cgaatcttat tcggcgtcac gaatcccaaa
    19441 atattttatc taaataaatg attttttcaa tggagtataa atgcaaaatg tcaaagtaag
    19501 tgaatggacg aaaaaagtga ttaagcccga tgagatcgaa aagtatctca tagaaggcaa
    19561 ggattttatt gatgaaaccg tcattacatc tcaacttaaa tccgcggcca atccggataa
    19621 atctcgcgtt cgtgagattt tagcgaaatc tttagagatt gaacggctcg aaccggaaga
    19681 gacggcaaca ttgctgaatg ttactgacga cgatctctgg gaagaaatgt acttagccgg
    19741 tctggcagtt aagcggaaag tttacgataa tcgtatcgta tttttcgctc cgctgtactg
    19801 cagtaatctt tgcgtcaata attgtgctta ttgcggtttc cgctacgaaa ataaagagga
    19861 gaaacgtcga attttaacta tgggaaaaat tcgcaaggaa accgaagccg tgattaacga
    19921 aggtcataag cgtatgatcg tagttttcgg cgagcacccg cgctcggctg tggactacat
    19981 tgccgaaacc attcgcacga tttacgacgt caaggtcccg gttaaaaccg gctttagtgc
    20041 gatccggcgc gtcaacgtca atgccgctcc gatgagtatc gccaatctga agaagctctg
    20101 ggaagtagga atcggtacat tccaggtttt tcaggaaacc taccacaaac cgacttatgc
    20161 gaatctgcat ccgcttaata ctatcaaagg taactatcgc tggcgtttgt attctttaca
    20221 tcgcgcaatg gaggctggaa tagacgacgt agccattggc gcgcttttcg gactgtatga
    20281 ttggcgcttc gaagttatgg gcttgctgta tcatacaatt gatctcgagc ggcatttcgg
    20341 catcggaccg catacgattt catttccgcg cttgacgcct gccagcggtt cctggttcag
    20401 tcgtaattca caatggcggg ttaatgacgc cgattttaag aaactggtta ccgttttacg
    20461 cttatccgtg ccttataccg gattaattgt caccgcgcgc gaaagtgctg aaatgcgtcg
    20521 cgaagttatc ggggtcggtt gtacccagac ggatgcttcc acgcgaattg acatcggtgg
    20581 ctatacttcc gctctgcaag ccctggcaaa aaaagaaaag gctgaccaac agattgatga
    20641 acaacaattt actctcggcg atacacgcag tctcgatgaa gtcgtccgtg aattggcgga
    20701 acaaggtctg atcacttctt tctgtaccgc tggctatcgt tgtggccgca ctggtggcaa
    20761 aattatgaat ttgttaaaag gttgtgtcga gggaaaattc tgtaaggcga atgccgtctt
    20821 gactttccgt gaataccttg acgattacgc ctcgcctgaa actaaaacgg ttggcgaacg
    20881 attgattcaa aaagaaatcg aggaaattaa agcgatgccg tttttccaga aagggcgaat
    20941 gctggaaact ttcgagaaac tctatcagca agttgccagc ggcgaacgtg atgtctatat
    21001 ctaaagaaat tttacaatca gccgcgagcc agactaacct accggataaa ctaaacgcaa
    21061 ttgccggaaa actggaagaa gaatatggtc tgcaagtcaa cttttgtaaa atttatggca
    21121 agcgctggtc gtatgtagcc ggtgaccgga accttgaatt tgctcctcag cgccgccaat
    21181 tgacttcgcg atatggaatt ttctataaac aaaacgacca aattaatcct gacaaaatga
    21241 acgagatttt tacagcacta acccaaatcc tgacggaaat cgaaaatgac tgcagaacac
    21301 agtagtccaa aatgttattt catttccaaa atctggcgga attgaaaata tgcaaagcac
    21361 ccccaaaagc aatcgtctcc aaatcggaat tttcggtcgt cgcaatgtcg gtaagtcgtc
    21421 tttgatgaat gcgctgacaa gtcaatatgt ctcaattgtc tccgatgtgg ctggaactac
    21481 caccgatcca gtcgaaaagc caatggaatt accgccgctt ggtccggtgc tttttattga
    21541 tactgctgga gtggacgatg aaggctccct cggcggacag cgtattgcgc gcacgcggaa
    21601 aatattggaa cgagtagatc tcgggattat cgttgccgaa gggaaaaatt ggaataaata
    21661 tgaagatgat ctgctgaagg aattgcaaaa tcggcacatt ccgaccgtag ttgctttcaa
    21721 taaatctgat ctatcacagc cttctgaaaa tctgcaggca tatttaaaac agcaaaatat
    21781 tccgtttatt aataccatcg caactgatgg tttcggcgtg ctggatttgc ataaaatttt
    21841 gattgatact actccggctg attactttaa ttccatcggg attattgggg atttggttaa
    21901 gcctggtgaa atggctgtgt tagttgtgcc gattgatcag gaagcgccca aagggcgttt
    21961 aattttacca caggttcaga cgattcgtga ccttttggat aataatgctg gttgcattgt
    22021 catcaaagaa aatgaattaa cctcagtatt aaataattta aagcaaccac cggcgattgt
    22081 ggtgaccgat tcgcaagctt ttctgaaagt tgccgccgaa accccagccg atataccatt
    22141 aacatctttt tcaattttat tcgcccgtta caaaggcgat ttggcagaat atattgctgg
    22201 aactcgaacg attgacaaac tcaaacccgg cgatgaaatt ttaattgctg aagcctgtac
    22261 gcatcatccc ataactgacg atattggtcg cgtaaaaatt ccccgctggt taaatcagta
    22321 tgtcggtgga caattgaact ttactcacta ccagggacac gatttcccag aggatttgag
    22381 taaatataag ctagtgattc aatgtggtag ttgtatgctg aataggaggg aagtcctatc
    22441 acgcatctgg tattgtaaac aagcgggggt tccggttacg aattatggat tgacgatcgc
    22501 ctatacgctc ggcatattcg aacgggcgac agcattattt aatattgagg cctaaaaacg
    22561 ctaaaagcgc gagttatacc aattttcttg gacaaatact tttcctatat tttttattaa
    22621 tttgatttta taaaatctat gcaaccaata ccgcaattgg ttatcaaaat aacaaaaaat
    22681 ggagataaaa tgaaaaataa atggatgtcg gtaattttga ttctactttt agcttccagc
    22741 ctattttcgg cagggaaaga atggtatagt tttaacgagg gtattgctct ggctaaaaaa
    22801 gagaataagc acatcgtact cgacttctac actgactggt gtggctggtg caaagttatg
    22861 gatcaaaaga ctttctcggt gccaaccgta aataaatatt tgtttgaaaa tttcgttcca
    22921 attcgaatca atgccgaaaa tgacaaggaa aagttggttt tccaaggtaa aacttatacc
    22981 ccccgtcaat tgaccagagc atttcaagtg accggttttc catcaattgc ttttattacc
    23041 ccagaaatgg aagtaatcga agtaattccc ggctacatcg aaaaagacct tttccttaat
    23101 ctattgaaat ttgtccatca ggaatgttat aaaactggaa tcagtttcga tgaattcgtc
    23161 aaaaacggat gccatggttc caaatagtcc ccatcaaact aaaatcgcaa aaatcttcca
    23221 aaatatctct ctgaaaaaac ttagatttac ctctaaaata tgtttatcag aactattttt
    23281 tggcaggtaa aatttgtaat atctaaattt gattgtttat gaatttggct gaaaaatagg
    23341 aagtataagt cggtgataaa gtcactacct ggaagagtaa aaacttcaaa atcgaaaaaa
    23401 gccaatttat atgccgatcg ccttcaagtg accagcaaaa tcataaaatc aggaataata
    23461 aatccaagtc tatctctttt tgattctcat ctatttaatt acattaatca aacaaattgt
    23521 aagccaataa ccaagaatac ctatgaaaga ttcaattcag aaaaatatcg ctaaccaaat
    23581 taataacaga gtaattccaa gatgggaatg gcgcactttc ggatcggaat tccccaaagc
    23641 ggaaaaattt ttgagttcac tcccatacga tactatcaag gagagtgaag aaatttacat
    23701 tttatcaaaa aattccaatg agaattgcaa aatccgcaat tcgttgatgg atattaagat
    23761 tatcgaacaa accaatcccg atggattgga attatggaaa ccagttctaa aggcttcatt
    23821 tccacttagt tttgaaatcg tacgtctcat actgcgcact ttacatgttc gtaagccgct
    23881 ggctatgcgc gatcattata ctcatcatca atttttaaat gaaatcgtcg catccaatcc
    23941 gaaactaagt ttggtagatg ttaaaaagaa gcgaaggtgt tacactctcg aaggggctga
    24001 agtcgaatta tccgaattgc aggttggcaa tcaatcagtt aagaccattg ccattgaaag
    24061 cgaaaaaccg caaaatgtgc aaaaagtact aaaattgctc gaattggata acctgcccaa
    24121 taccaattac atcaaagctt tgaaaaaaat gaacaggcac taatatggga atagaaatag
    24181 aaagaaaatt tctagtaatt gccaatgatt atcgtacttc tggcaacggt gtccgttatc
    24241 gtcagggtta tctcagcaca gccaaagagc gagtggtacg ggttcgaaca gtcggtcaac
    24301 aggcattttt gacaattaag ggcataaacg tcggagccgt gcgaccagag tttgaatacg
    24361 aaattcctag gcaagatgct gaacaaattc tcgaaaatat ctgcgaaaag cccattttag
    24421 acaaaatccg gtatcgagtt aagcatagcg gatttatctg ggaagtggat gaatttctgg
    24481 gagataatgc tggattagta atagccgaga ttgaactcgc cgacgaaaac caatcattta
    24541 ttaagccgga ctgggtggga gctgaggtga ccggtgatcc gcgttacttc aattccaatt
    24601 tgataaaaaa tccctacaag tcctggaata ttaaataatc ccaaaataaa gtatctttta
    24661 aattattgtt tggtttgttg gtaaaataag tggtacctat ctgaaaatat tttgaggaaa
    24721 cttcgctaaa aatattaaat taacgccgct atttggagag gtggcagagt ggttgaatgc
    24781 ggcggtcttg aaaaccgttt ctcgacttat cgggacgggg gttcaaatcc ctccctctcc
    24841 gcaaatgaac gtggttgaat gcggcattct tgagaaccgt ttaccgacat ctcgggacgg
    24901 gggttcttcc gtaggaactc cgctggagaa atccctccct ctccgc
//
LOCUS       DAOOMU010000024        24682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1900_length_24682_cov_20.639907, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000024 DAOOMU010000000
VERSION     DAOOMU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 24682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24682
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1900_length_24682_cov_20.639907"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1384
                     /locus_tag="PLI55_03655"
     CDS             98..1384
                     /locus_tag="PLI55_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HPA99966.1"
                     /translation="MVEIVGIKPNSLAEMNGIRRGDQIVSINRQPINDFLDLYFHQSD
                     ENCKVVVLRDGHKKTFQIAKNADEPLGIELSPPRLKACGNNCIFCFIKQNPPGMRPAI
                     YFCDEDYRYSFLLGNYITLTNLSEADLNRIVLQRLSPLYVSIHATDENVRRHIFQLKK
                     PDNLMQKLTFLAQNRIDLHTQIVLIPGVNDGAVLEKTLADLYIFRDSILSVAIVPVGL
                     TAHRQHLPVIPAVDSHLASQLLENIRTWNRRYVNRDGEHWVFLSDEFYLLVGQDLPGC
                     KFYGSFYQIENGVGTTRAFLDDFKRQSRKLPHAISKRKRVLFVTGMLAAPVLSRYVTA
                     RLNQINNFSVDILPVINNFFGPSVTVAGLLTGQDILAQAINVDEYDLIVLPPRIINED
                     GVLLDDFYPHQIAEALHRPVQVWDGDFGKLVDEDNG"
     gene            1377..2690
                     /gene="der"
                     /locus_tag="PLI55_03660"
     CDS             1377..2690
                     /gene="der"
                     /locus_tag="PLI55_03660"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPA99967.1"
                     /translation="MAEPIVAIVGRPNVGKSTLFNRLTQKRKSIVHSEAGITRDRVYE
                     TVNWAGRQFLLIDTGGFIPESTNQITKAIREQVRLAIEEANLVIFVVDASEPMTALDR
                     EIAEMIRRSQRDCLIVANKCDNERLEVNSLEFTELGLGDVFPISALNGRHIGDLLDAI
                     LQRIPRKIAANDDEKDHLQLAIVGMPNVGKSSILNVLAGSEKAIVTDVPGTTRDAIDT
                     KIKYFNEPVTLIDTAGMRKHRQIKDEVEYYSLLRAYRAIERCNVALVVVDAVKGFTRQ
                     DAAIVRQVIDLRKGLVIAINKWDLVQRETNTQVNFKRNLIEQFRELEHYPIIFISAKT
                     HQRVHKILETARMVYENRKIRIGTSELNQFLQDVIDRTPPPANLGKFIKIKYITQVKT
                     EPPVFVFFCNEPRLIKEEYRRFLENQLRRRFGFSGVPLIISFRNK"
     gene            2687..4366
                     /locus_tag="PLI55_03665"
     CDS             2687..4366
                     /locus_tag="PLI55_03665"
                     /inference="COORDINATES: protein motif:HMM:NF039970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6055 domain-containing protein"
                     /protein_id="HPA99968.1"
                     /translation="MKIVRQLTFTIVCLIVVEQALAIDQIELIATDESYWQALNAVKN
                     DLRPRRELLKQPVVGYKYSRPSLPLSYRSAGSHFLIHYTLEGYDAVDATITNADGVPD
                     FVYETARAAERSYSLLIDTLGFQSPPVDDIFSPETDIYIINLGGAAYAYTYDENPVQT
                     IPDRPYDYTAYTVIDNDFVGYLTTGLNGLRVTVAHEFFHVVQLGYNWWEGNGLANSGG
                     GRTSADDYFLEWCSTWFEERAYPEVNDYLFYVGAFFNSPLKSLWNYDYAYALGAFLRF
                     MLDRYGENLLVKVWEEIKTNYAFESLQAVLADDYHADLADLWNEFYLRCYFTGERYDP
                     DWALSSDAMQFPLLQIPATNKVVFTESAVFTPSIEPFACVPYQVVFDQNWLCGINVNS
                     SSPENFLGRYLFIKNRVGKIAETLSLNQNLLVGNAANNDSLLIFMTNSSKTTTPNLTV
                     TISEVGDEFRIASTIKNVYPNPYSPGKSGDLIVDLQVGQIVNSIKTNWFDLTGRNVFQ
                     KNFDNGLIEIGNYSLSFSSDELRQAQLASGVYIMQIAIGSKNLTRKILLLK"
     gene            4378..4989
                     /locus_tag="PLI55_03670"
     CDS             4378..4989
                     /locus_tag="PLI55_03670"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPA99969.1"
                     /translation="MAEMLPQELKTIADSAEIMLTVKDSKFIGYCFPVADKSEAVQKI
                     AEISQKHRKATHICWAYRIYENNQLLYYAHDAGEPRGSAGAPILKALTSRQLVKALVV
                     VVRYFGGTKLGIGGLIRAYGATAGKTLDLVEHKPFIQRQTLRIECPLAKYSQLAHFLG
                     RFSESSKPEFTENNVIVTVQIEVQKADEFRTELNSIPGLIITT"
     gene            complement(5291..6070)
                     /gene="mazG"
                     /locus_tag="PLI55_03675"
     CDS             complement(5291..6070)
                     /gene="mazG"
                     /locus_tag="PLI55_03675"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPA99970.1"
                     /translation="MNDELSRYFDELIEIVKTLRGPNGCEWDKAQTRDSLTPYLLEEA
                     HELIDSISENNPSKIKEELGDVLLHIIFQAQIAAEAGEFTIADALQQINAKLIRRHPH
                     VFGETKVENVNDIKKNWEEIKLKEGRQSLMDGLPKSLPALLQARRVQERAAQVGFDWS
                     TIEPIWAKITEETRELQEAIATRNKDEIVAEFGDLLFTMVNLSRFLDLDPEEALRQTV
                     TKFIRRFQAIENELAATGKNFKDTSLEELDKIWNKSKDKES"
     gene            complement(6063..6527)
                     /gene="ybeY"
                     /locus_tag="PLI55_03680"
     CDS             complement(6063..6527)
                     /gene="ybeY"
                     /locus_tag="PLI55_03680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPA99971.1"
                     /translation="MTIVKIFNNHSHYTLLKPAIEQVVSYIVNMEQQAVQSITFVASD
                     DKTLNEMKVKLFGIDQLTDTISINYNEIDQPVEGEIYLSLDRIEDNARTFKVTFEKEL
                     ALVIIHSVLHLLGYNDDNLNDKKQMQALQNFYIQQIKFPRLYRQRRIQPNYE"
     gene            complement(6505..>7600)
                     /locus_tag="PLI55_03685"
     CDS             complement(6505..>7600)
                     /locus_tag="PLI55_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPA99972.1"
                     /translation="ATICLIIGAQIGGNFNFIIVSVFVSTFAIYAVRQLRKRSQIFKS
                     IIYIVLGYCLAIFATEMLKYSPPKSILNNIFYATINGVFAPFLAYGIIGLLENLFGIT
                     TDLSLLELADFNQPLLKLLSHEATGTFTHSVTVGNLAEAAADAIGANALLARVGSYYH
                     DIGKLTKPEYFIENQAFDYNRHDKLAPNLSAIIILNHVKEGLRLANEYKLPQVIQDFI
                     STHHGTTRVEYFYDKALKKSAKPDEINDADFRYPGPKPHTKETGIVMICETIEAVCRS
                     LNKPTMSAIEKVVDSVIESRLKEGQLDECPLTLADLKKIKGDLKEGTGILPILRGIHH
                     LRVEYPGQEKFSNSSVPSNKKGKNDDSQNL"
     assembly_gap    7601..7700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7701..9047)
                     /locus_tag="PLI55_03690"
     CDS             complement(<7701..9047)
                     /locus_tag="PLI55_03690"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99973.1"
                     /translation="MANSNSSKIAGKERGVFQSRRQRTGVFLICGVIVVALALLFPKT
                     HYNITKYKINDIARSSIIAPFDFPILKTEQELEADRLEAIKKVPFVFIQDKDVEKTEL
                     MNLQKFFTEIDRLRLARIKYDESLRNISINRSEESLAILTADSITFFALQKKFLNDYK
                     LNKNSPVLKSLVYQYDPKNVNQLSAHVQNQVKSVLSELYVLQILDIPKSQIISEKIAI
                     LQGGEEVLENPNEVLELSEAWTKAKLALQARLNDYTPEVVNAAYDIVVAFLKPNLIYQ
                     KQITEKRQAEAIAKVPISKGVVLKNEKIVDANTRITPEIYQKIESMAKEQARLANVQR
                     SWRRALPVIGDPAIFICQVAVVAIIFTFFITFLFAYRTDIITSPKKVFLIGIIFVLQA
                     VFAAILVNHFKISEYTIPITLAAMSLTILFDSRVAFVGIATICLIIGAQIGGNFNFI"
     gene            complement(9076..10068)
                     /locus_tag="PLI55_03695"
     CDS             complement(9076..10068)
                     /locus_tag="PLI55_03695"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPA99974.1"
                     /translation="MYKSKIASLGKNVPENIVTNADLEKLMDTSDEWIVERTGIRERR
                     FVTPPCGASQLALPAVLEAITNAGLTKNDIDLIIVATTTPEHLTPGTSSYLQALLELP
                     NIPTLDIRVQCSGFIYGLAIADAFIKTGQYKNILLVGVEVQSVGLNLTTAGRDIAVLF
                     GDGAGAAVITRSTDESAILSTHLFGDGRYANELWVEAPDGSKRPRLSKEMLDEGKHYP
                     KMNGRQVFKHAVTKFPEVINIALEANNLNKSDITLIIPHQANLRITEAVAQRLEIPMS
                     QVYSNIHKYGNTTAASIPLAMYDALQEGRYKKGDYLILAAFGSGFTWASAALRW"
     gene            complement(10093..11799)
                     /gene="recJ"
                     /locus_tag="PLI55_03700"
     CDS             complement(10093..11799)
                     /gene="recJ"
                     /locus_tag="PLI55_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPA99975.1"
                     /translation="MKQKWLISNPNPNAVHQLAGELQIPPAIVRILLNRGLFDKDSIE
                     KFFSPSTQDTHDPFLMADMTKAVERLGQAILRREKVLIFGDYDVDGTTATSLLYLVFH
                     EIGLDTAFYIPDREREGYGLSQLGIEYARQIGATLIITCDCGINAFDTIDYANSNQID
                     VIVTDHHEPAEKLPAALAILDPKREDDHYPFKELSGVGVAFKLLQGFHIANNLPVTNL
                     YRHLDLVAIGTAADIVPILGENRVLVAKGLEYLNSSQKVGLQALLKTSGFDSKTMNVV
                     NIVFGLAPRLNAAGRLGEASRAVHLLTSFDRKEAFELANLLEQENRNRQAIERETIDS
                     AILQLNATHDLDADKILILSNTNWHHGVIGIVASKLKELYNRPVVMISFQNGIGKGSA
                     RSIPGFDLYSAFNSCADLLENYGGHKMAAGLTILIESLPEFTRRLKKIAAEQIDEQML
                     NSGLQIESEIDFNDINPQTLSFLHKMAPFGPGNMRPVFCTRNVTVSGMPRIISENHLK
                     FKAAQNRSVLSAIGWKMGCYYEMLISNRPLDIAFVIDENEWNGYKEIQLNIKDIVYSD
                     YH"
     gene            complement(11810..12197)
                     /gene="rnpB"
                     /locus_tag="PLI55_03705"
     ncRNA           complement(11810..12197)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLI55_03705"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(12196..12996)
                     /locus_tag="PLI55_03710"
     CDS             complement(12196..12996)
                     /locus_tag="PLI55_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPA99976.1"
                     /translation="MFKELERLVEVQKIDIKLLEINNLRGDLPQIVENLQAQIDTLSK
                     ELENERKREQEITREIKELESKIADDRILLNRYQDQLYLVTSNKEYDALTSEIDNLKQ
                     EIDNAEYNILKLSDEIERLKISIKSKELTLEEKTKELDQKQSLLDKTDITTKDAQEAL
                     LEQRKRIVDLIPIRYMREYERIAKAKNGIAIVPILQIFEEKVDKKGNVEYIPMQVSCG
                     GCSKIVPPQKILEIRSGDRINRCEFCGRILYWDEQSSEIRSNYEEELF"
     gene            complement(13105..13178)
                     /locus_tag="PLI55_03715"
     tRNA            complement(13105..13178)
                     /locus_tag="PLI55_03715"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13142..13144),aa:Val,seq:cac)
     gene            complement(13294..14682)
                     /locus_tag="PLI55_03720"
     CDS             complement(13294..14682)
                     /locus_tag="PLI55_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HPA99977.1"
                     /translation="MTSLRLAPIDLLIIAIYLVGIFLIGLKYSRNYRGNVDDYLLAGR
                     RLSLPAFVATLVSTWYGGILGVGEFTYRYGLLNWVTQGLPYYFFAILFGIFLAPRIQR
                     SGLYTIPDQLYQRYGKPAGFLGSVFVFLLTNPASHLLMSTLLLSAIFGISFWLAFLIG
                     VIFSLFYVFMGGMKSDVLTDFFQFGLMFLGFGVLAVKLITNYGGLNFLQANLPQNHLR
                     FTGGENWQYIMVWFFIAFWTFVDPGFYQKCYAARTPQIARRGVLISVIFWLLFDMLTT
                     TCGLYARALIPDTNPLMAFPKLGEMALGDFWRGIFFIGLLATIMSTLDSVALLSAMTF
                     GRDLLWRLKPNSNINRNTQIGFFVIIGLSILVILFLPSVVKIWYTFGTLFIPALLIPV
                     ICNFTKYNYKSSVMFTGMLGSFFATLIWFIIGMRNGSIMEPIYLFNIQPFFIGLLSWL
                     LFLPFLVDRFTT"
     gene            complement(14679..15347)
                     /locus_tag="PLI55_03725"
     CDS             complement(14679..15347)
                     /locus_tag="PLI55_03725"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HPA99978.1"
                     /translation="MTPNTPINFLSPTALVLGNGNYPNWFDIKQFQVEYKVLLVCADG
                     GAEKARQLNLMPDLIIGDMDSVTTDTINHFQSSEVKIIHQPNQNNNDLEKCLRWLLRH
                     GWRQFVLVGFTGRRDDQTIATLQIMRKYARRAQFLLYTETAQIYLLKKGIWLINAIPG
                     QTISLFGFPRVYHLTTRGLQYPTKDENLTGGSHGLSNTAIDEMVQIQFSSGYLLVVAI
                     QELN"
     gene            complement(15362..16282)
                     /locus_tag="PLI55_03730"
     CDS             complement(15362..16282)
                     /locus_tag="PLI55_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99979.1"
                     /translation="MTRFRKEYSKIARKLTSADLQSGIGNSELSLDNIVSDGRLAVDS
                     QFFMGVFSRQGLLNAIRAFGLLDILERKGLTNITIDIDTSDPYIHRLYAYSGKPLNEN
                     KICELVLKQGPLHFENKPLSSFPRRTANILYIEWLLLQNPREKFSPEKPPLPGQNYPG
                     LGIGDRLMEIMILMTRRLNLEGIINKPHYFHTAFMFSKEFVFINPYNQALMYAISKDL
                     LAKYSFWMVAWAAHFNCIINKNDGKPMDWKTGDLILPFTKDLIKYFRGREYQQEVSRL
                     IANFSFEIDLDKLHTALKSNDLEKYARFPK"
     gene            complement(16309..17433)
                     /gene="selD"
                     /locus_tag="PLI55_03735"
     CDS             complement(16309..17433)
                     /gene="selD"
                     /locus_tag="PLI55_03735"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPA99980.1"
                     /translation="MADAVSAISQTYHRLTSTEEISIPITSNEPIRLTQFTHGLGCAC
                     KIRPQTLEKILREIRPRMALNVLVDFSASDDAAIYKINDEQAIVATVDFFTPIVDDPY
                     DFGRISAANSLSDIYAMGAEPLFALNVVAFPEKRLPVEILTQILRGAQVIADQAGIPI
                     LGGHTVEDNEPKFGLVAIGIIHPEKIIRNIGAQPGDALILTKPIGVGILSTALKRGLL
                     DELTKNLIIANMTELNATAAEVMKSFPVHACTDITGFGLLGHLHEMTTGSRVRAQISA
                     SSVPILPSTNEMVLRNVIPGGTEANLSFISPMVIWDKMITDSDKIILCDAQTSGGLLI
                     AVADEFANDMLAQLHQAGVINAAKIGKIESGADGKIKVVP"
     gene            complement(17410..18507)
                     /locus_tag="PLI55_03740"
                     /pseudo
     CDS             complement(17410..18507)
                     /locus_tag="PLI55_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699653.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
     gene            complement(18616..19587)
                     /gene="nadC"
                     /locus_tag="PLI55_03745"
     CDS             complement(18616..19587)
                     /gene="nadC"
                     /locus_tag="PLI55_03745"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPA99981.1"
                     /translation="MNNSVKNFTDSRTKDVKQCFDISTELRLENKYYRKFALTFFDNL
                     LDFDISSKDASLQPAKYYRRQVTAVINAKAPAIIAGMAEIQAFLDHHNLLSTSDFKDG
                     DPVKGGETIMTIEGRADRILTVERTVLNIFQRLSGIATVTAEYSQALKGLPCFVTATR
                     KTLWGIVDKSALQYGGGLTHRLGLADAAILKENHLTVLKNSGDPQALMTALDAIVATD
                     PFLRFVEVEVTNDDEFWQVVEAYNRLRPETYCVIMFDHFAPENIAQLIKATKNNGVYP
                     TIFFEASGNITLETIRDYAASGVDVISVGALTHSVKSADFSLLINKF"
     gene            complement(19580..21091)
                     /locus_tag="PLI55_03750"
     CDS             complement(19580..21091)
                     /locus_tag="PLI55_03750"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPA99982.1"
                     /translation="MKTKLTLLLSWGIMASILLSNCGSKIDASRCVLLPVAEDPTVSY
                     RIYFKVGSQDDPAGKEGLAFLTAHMLSEAATQSNSYEEIIAKLFPLAASYDATVSAEM
                     TVISGRVHRDNLPAYYPLFTEAILKPAFQEDDLNRLKSNVLNYLENVLKYANDEELGK
                     AVLYNEIFAGTGYGHPTEGLINSINSITINDVKDFYQKMYNRNNVIIGVGGGYDNETL
                     AKLQKDLAQLPRGVENIATRAVPYDIQGLKVTIVEKDANATAISLGFPINITRENPDW
                     YALAVANSWFGEHRNSSSHLYQVIRETRGLNYGDYSYIEHFPNSGYYQFPPVNVARHQ
                     QIFEIWIRPVPNETRHFVLRATMRELHNFIENGLTAEQFNLTRNFLRKYILHYAPTTM
                     MRLGYAIDDKFYGIKGSHLENFCIALDNMTVNDVNSAIRKYLQYDNLQIAIVTNEAEK
                     LREDLLANTPSPIKYITPKTEEVLKEDLEIANYPLAIRPENIKIVNVRDLFYE"
     gene            complement(21088..22422)
                     /locus_tag="PLI55_03755"
     CDS             complement(21088..22422)
                     /locus_tag="PLI55_03755"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPA99983.1"
                     /translation="MLKEISLGIILMFSLWVTGQTAEPFFPYKYNVEVLPNGLKVITI
                     PMESNGIVAYYSITRTGSRDEWEPGHSGFAHLFEHLMFRGTEKYPGSLYDKTITELGA
                     DANAYTTDDYTCYHLNFAAEDLEKIIEMESDRFINLAYKEDEFKTETGAVYGEFLKGR
                     VSPWFVLYEKLYDVAFDKHTYKHTTIGFEADVKAMPTMYKYSKTFFQRYYRPENVVLL
                     IVGQIETEKTLELVRKYYGDWQPGYVSPQIEPEPPQTAERKAEVIYPGKTLPIVMVAY
                     KSDAYDPNNRLYVAGLLLEQLAFGENSDLYKRLVLKEQKVEFITADFSVSRDPKLNEI
                     YAMVKSPDDIDYVREQIFMTMEQFKTEAVPESRLEDLKKHLKYSFLMNLDTPDNVAGN
                     LANYVAITGGVETVDQLFNQIMNVTVTDIQQAAQYYGVPEKRTVVILKGVAQ"
     gene            complement(22556..24355)
                     /gene="glmS"
                     /locus_tag="PLI55_03760"
     CDS             complement(22556..24355)
                     /gene="glmS"
                     /locus_tag="PLI55_03760"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPA99984.1"
                     /translation="MCGIVAYIGNQSAVPLLLEGLKKLEYRGYDSAGICIAGDHRLEV
                     VKRSGKIAELEKTAALDSFSSHIGIAHTRWATHGAPNEINAHPHLSCHGEIAIVHNGI
                     IENFRSLRDMLIQEGHHFSSETDSEVLAHLIEKFYEGDLATAVVKALHNVEGTFGIAV
                     MHKDEEHLVIARRGSPIIIGLGNQETIVASDAVSIIPHTKKVIYLNDNEIAVIGYNNY
                     RLQNLDGQSIANEIQEIRWNISQIEKRGFKHFMLKEIFEQPEAVQNTLRGRITNGQIK
                     LSLNTDFNRVQRIIIAACGTSWHSALIGKNLIEKYTGIPTEVDYASEFRYRESIINGE
                     VLMIVISQSGETADTLAALRKAKSAGTQTLGIINVVGSTIAREVNSGIYLHAGPEIGV
                     ASTKAFICQVIALLLLALYIKQQKGELIDPALLAKIDMIPEQIRQVAEHSDNLREIAS
                     AYYLNRNFIYLGRGINYPVALEGALKLKEISYIHAEGYPAAEMKHGPIALIDENMPVV
                     FIATKGAVFDKVLSNMQEVKARGGQILTITDHNNPHLQELSNHLIIVPETSEDLSPLI
                     NVIPLQLLAYHIADLKGLNVDKPRNLAKSVTVE"
BASE COUNT         6777 a         5075 c         5601 g         7129 t
ORIGIN      
        1 attaattttg tttttccggt cgggattagc gaattttgga cagtttttat taatatgaag
       61 caatttttaa ttaattataa aatatggtgc tggaatcgtg gttgaaattg tcggaataaa
      121 gcccaacagc ctggctgaaa tgaacggtat ccgtcggggc gaccagattg tcagcatcaa
      181 tcgccagccg atcaacgatt tcctcgattt gtattttcat caatctgatg aaaattgtaa
      241 agttgttgtc cttcgggatg gtcataagaa aacattccag atcgctaaaa atgccgatga
      301 accactgggg attgaattat ctccgccgcg cctgaaagcc tgtggaaata actgcatttt
      361 ctgctttatc aagcaaaatc cgcccggaat gcgaccggca atttatttct gcgatgagga
      421 ttaccgctat tcgtttctac tcgggaatta tatcacccta accaatttaa gtgaagccga
      481 tcttaatcga attgtcttgc aacgcctttc gcccctgtac gtttcgattc atgcgaccga
      541 tgaaaatgtg cgacgccata tttttcaatt gaaaaagccg gataatttga tgcaaaagct
      601 cacttttcta gcccaaaacc ggattgatct acatactcaa atagtactaa ttccgggtgt
      661 taacgacggg gcagtactcg aaaaaacgct ggcggattta tatattttcc gggattcgat
      721 attatcggtg gcaatcgtac cagtcggtct gactgcgcat cgccaacacc ttcccgttat
      781 tccagcggtg gattcgcatc tggcatctca attgcttgaa aatattcgaa cttggaatcg
      841 ccggtacgta aatcgcgatg gagagcattg ggtttttctc tccgatgaat tttatttgtt
      901 agtcggtcaa gatttaccgg gatgcaaatt ttatgggagt ttttaccaaa ttgaaaatgg
      961 ggtcggaacg acgcgggcat ttcttgacga tttcaaacgg caaagtcgta aattaccaca
     1021 cgctattagt aagaggaaaa gagtgctttt cgttacaggt atgctggcgg caccggtttt
     1081 aagtagatat gtcacggcgc gattgaatca gatcaataac ttctcggtgg atattttacc
     1141 agtaattaat aatttctttg ggccatcggt aacggtggca ggattattga ccggtcaaga
     1201 tattcttgct caggcaatca atgttgacga gtatgactta attgtgttac caccgcggat
     1261 tattaatgaa gatggagttt tactcgatga tttttaccct caccagattg ctgaggcttt
     1321 gcacagaccg gtacaggttt gggatggcga tttcggtaaa ttagtggacg aagataatgg
     1381 ctgaaccgat tgtagcaatt gttggacgac cgaacgtcgg caaatcaact ttattcaatc
     1441 gcctaaccca gaagcgtaaa tctatcgtgc attcggaagc cggtattacg cgagatcggg
     1501 tttacgaaac tgtaaattgg gcgggacgac aatttttgct gattgacacc ggcggcttta
     1561 tcccagaaag caccaatcaa ataacgaaag caattcgtga acaggttaga ttagccattg
     1621 aagaagccaa tctggttatt tttgtagtag atgccagtga accgatgaca gccctcgatc
     1681 gtgaaattgc cgagatgatt cgacgctccc agcgggattg tctaatcgtt gccaataaat
     1741 gtgacaatga gcgactggaa gttaatagtc tggaatttac cgaattaggc ttgggcgatg
     1801 tttttccgat ttcggctttg aatggacgcc atatcggcga tctactggat gccattctgc
     1861 agagaattcc acgaaaaatc gccgctaatg atgatgagaa agaccatttg caattagcaa
     1921 ttgtcggtat gccgaatgtc ggcaaatctt cgattttaaa tgtcttagcc ggctcggaaa
     1981 aggctatcgt aaccgacgtt cccggcacga cccgcgatgc gattgataca aaaataaaat
     2041 attttaatga accggttact ttgatagata ccgccggaat gcgcaagcac cgccaaatta
     2101 aagatgaagt agagtattat agtcttctgc gtgcttatcg ggcaatcgag cgctgtaatg
     2161 tggcgctggt agttgttgac gcagttaaag gatttacccg tcaggatgcg gccatcgtcc
     2221 gccaggtcat tgacttgaga aaaggcttgg taattgcgat taataaatgg gatttggttc
     2281 agcgcgaaac taatacccag gttaatttca aacgtaattt gatcgaacaa tttcgggaac
     2341 tggaacatta tccgataatt tttatttcag ctaaaactca ccagcgcgtg cataaaattt
     2401 tagaaactgc ccggatggtc tatgagaatc gcaaaatacg cattggaaca agtgaattga
     2461 accaattcct gcaggatgtc attgaccgca caccgcctcc ggcaaatctg ggcaaattta
     2521 tcaagataaa atacatcacc caggtcaaaa ccgagccgcc ggtttttgtt tttttctgta
     2581 atgagccgcg cttaatcaaa gaagaatacc gacgcttttt agaaaatcaa ctgcgccgcc
     2641 ggttcggttt ttctggagtg ccactgataa tttccttccg gaataaatga aaatagtccg
     2701 tcaattaact tttacaatcg tatgtctgat tgtagtcgaa caagccctgg caattgatca
     2761 aattgaattg attgccactg atgaaagcta ctggcaagca cttaacgccg tcaaaaatga
     2821 tctgagacct cggcgcgaat tgctcaaaca gccggtggtc gggtataaat attcgaggcc
     2881 gtctttgcct ttaagttatc gatcagcagg aagccatttc ttgattcatt acacattgga
     2941 aggctacgat gccgttgatg cgacgattac aaatgctgac ggggtgcctg attttgttta
     3001 tgaaactgcc cgagccgccg agcggtctta tagtttgcta attgatacac ttggttttca
     3061 atcaccgccg gttgatgata tatttagtcc agaaaccgat atttacatta ttaatctggg
     3121 tggggccgcc tatgcttata cctatgatga aaatccggtt cagactattc ccgatcgccc
     3181 ctatgactat accgcctata cggtaattga taatgacttt gttggatatc tgacaacggg
     3241 cttaaatggt ctgcgagtca cagttgccca tgaatttttt cacgtggtac agctcggtta
     3301 caattggtgg gaaggtaacg gcttggcaaa ttcgggtggc gggcggacaa gcgccgatga
     3361 ttatttcctg gaatggtgct cgacctggtt cgaagaacgg gcttatccgg aagtgaacga
     3421 ttatctcttt tatgtcggcg cttttttcaa ttctccactt aaatcgcttt ggaattatga
     3481 ttatgcctat gcgctgggag cttttcttag atttatgtta gatcgttatg gcgagaatct
     3541 cttggtcaaa gtctgggagg agatcaagac taattatgct ttcgaatcgc ttcaggcggt
     3601 tctggcggat gattatcatg ccgatttagc cgatttgtgg aatgagtttt atctccgatg
     3661 ctattttacc ggagaacgct atgatcccga ttgggcgctt tccagcgatg ctatgcaatt
     3721 tccattgtta caaattccgg caactaacaa agttgtcttt acagaaagtg cggtttttac
     3781 tccatcaata gaaccctttg cgtgcgtgcc atatcaagta gtgttcgacc agaattggtt
     3841 atgtggaata aatgtcaatt catcgtcgcc ggagaatttt ctcggacgtt acttgttcat
     3901 taaaaatcgg gtcggaaaaa tagccgagac attgtcactt aatcaaaatt tattagtcgg
     3961 taatgccgcc aataatgatt ctctacttat ttttatgact aatagcagta aaactacaac
     4021 tcctaatctg actgtgacaa tttccgaggt gggtgatgaa tttcgaatag cgtcaactat
     4081 taaaaacgtc tatccaaatc cgtattctcc gggaaaatcg ggagatttga tagtagatct
     4141 acaggtgggt cagattgtta attcaatcaa gacgaactgg tttgacctta cgggacgaaa
     4201 tgtatttcag aagaattttg ataatggact gatcgagatt ggtaattatt cattatcatt
     4261 ttcgagtgat gaattaagac aggcgcaact tgcatccggt gtctatatta tgcaaattgc
     4321 gatcggaagc aagaatctaa cgcgcaaaat tttactactg aagtaaatat aaaatttatg
     4381 gctgaaatgt tacctcaaga gctaaaaaca attgctgatt cggctgaaat tatgcttaca
     4441 gtcaaagatt caaagtttat cgggtattgt tttccggttg ccgataagtc cgaagcagtc
     4501 caaaaaatag cggaaattag ccagaagcac cgaaaagcta ctcacatttg ctgggcgtat
     4561 cgaatttatg aaaataatca attgctatat tatgctcacg atgcgggcga gccacgcggt
     4621 tcagccggag cgccgatctt aaaagcgcta accagccggc agttggtcaa agcgcttgtc
     4681 gtagtcgtgc gatatttcgg cgggacgaag ctgggaatag gaggcttgat tagagcatac
     4741 ggcgctacgg ccgggaagac gctcgatctt gtcgaacaca aaccttttat ccagcgccaa
     4801 accctaagaa tcgaatgccc gctagcgaaa tactcacagc tggcacattt tctcgggcgt
     4861 ttttcggaat cgagcaagcc ggaatttacc gaaaataacg tcatagttac ggtgcaaatt
     4921 gaagttcaga aagcggatga gttccgaacg gaattgaact cgattcctgg tttaattatc
     4981 actacataaa aatatcctgc aacagcgctg gcggattaag ggaaccgtga aaatcacggt
     5041 gggtacctcc cgaacggatc aagtttcttc aacgaggaag cgtgctttag ccgttcggag
     5101 tccggctccc aagtttatgg tgttggttcc aaaaacactc gccacactat tgcaggataa
     5161 tttttcaata agcgtttcat acataaacaa tatagcatta attaagatat tcgtcaatag
     5221 gttcggcgaa aaatttcatt atattgtggg aatgattttt tactaaaccg agagttagtg
     5281 gccttaatat tcaggattct ttatctttcg atttattcca gattttatcc agctcttcca
     5341 gcgaggtatc tttgaaattt ttaccggtgg cggctaattc attttcgata gcctgaaaac
     5401 gccggataaa tttagtgact gtttgtcgca aggcttcttc cggatcaaga tcaaggaagc
     5461 gggaaagatt taccattgta aaaagtaaat cgccgaattc tgcgacgatc tcatctttat
     5521 tacgagtagc aatggcttct tgcaattcac gcgtttcttc ggtgatttta gcccagattg
     5581 gttcgatggt agaccagtcg aaacccactt gagccgccct ttcctggacg cggcgggctt
     5641 gtagtaaagc cggtagagat ttgggcaacc cgtccatcag ggattggcgc ccctctttca
     5701 gcttaatttc ttcccaattt ttcttgatgt cgttgacatt ttcaacttta gtttcgccga
     5761 aaacgtgcgg atgacgtcga attaacttgg cgttgatttg ttgcaaagcg tcggcaatgg
     5821 tgaattcacc ggcttcggcg gcgatctgag cctgaaaaat tatatggagc agtacatcgc
     5881 cgagttcttc ttttattttc gaggggttat tttcagaaat ggagtcaatg agctcgtggg
     5941 cttcttcgag caaataaggc gtaagagaat cacgagtctg ggctttgtcc cattcacaac
     6001 cattgggacc gcgcaaggtt ttcacgatct cgattagttc atcaaaatag cgggaaagtt
     6061 catcattcat aattaggctg gatacggcgc tgacggtaaa ggcgagggaa tttaatttgc
     6121 tggatataaa aattctgaag agcttgcatt tgttttttgt cgtttaggtt gtcatcatta
     6181 taaccgagta aatgtaaaac gctgtgaata attaccaggg cgagctcttt ttcaaaggtg
     6241 actttaaatg tgcgggcgtt gtcctcaatg cgatctagac tgagataaat ttcaccttct
     6301 accggttggt ctatctcatt gtaattgatg ctgattgtat cggtcagttg atctatcccg
     6361 aataatttga ctttcatttc attaagagtt ttatcatcgc tagctacgaa ggtgatgctc
     6421 tgaacggctt gttgctccat attgacaatg taagagacaa cctgttcgat tgccggtttt
     6481 aaaagggtgt aatgagagtg gttgttaaag attttgacta tcgtcatttt ttcccttttt
     6541 gttacttgga acggaagaat tggagaattt ttcctgccca ggatattcca cacgcaggtg
     6601 atgaatgccc cgtaaaattg gcagtattcc ggtaccttcc ttgagatcgc ctttgatttt
     6661 ctttagatcg gctaaagtca gcgggcattc atcgagttga ccttctttga gtcggctttc
     6721 aatcaccgag tccacgactt tttcgatagc cgacatagtt ggcttgttga gagagcgaca
     6781 taccgcttcg attgtttcac aaatcataac gatgccggtt tctttagtat gtggttttgg
     6841 gccgggatag cgaaaatcgg catcattgat ttcgtcgggc ttggcggatt ttttgagggc
     6901 tttatcgtaa aaatattcaa cgcgagtagt accatggtga gtagaaatga agtcctgaat
     6961 aacctggggt aatttatatt cgtttgccag ccgcagaccc tccttgacat ggttaaggat
     7021 gataatcgcg ctgagattgg gcgccagttt atcgtgccgg ttataatcaa aagcctggtt
     7081 ttcgataaaa tattcgggtt tagtcaactt gccaatgtca tggtagtagg aaccgacgcg
     7141 agccaatagg gcatttgctc caatagcatc agcggcggct tcagccagat tgccgaccgt
     7201 tacactatga gtaaaagttc cagttgcctc gtgcgaaagt aattttagga gcggttgatt
     7261 aaaatccgcc agttcgagta gtgataaatc agtggttatt ccaaaaaggt tttccaatag
     7321 cccgataatt ccatacgcta ggaacggtgc gaaaacgcca ttaatcgtgg cgtagaaaat
     7381 attatttagg atagatttcg ggggcgagta tttcaacatt tcggtggcga aaatagccag
     7441 gcaatacccg agtacgatat agatgataga tttgaaaatt tggctacgct tccgcaattg
     7501 ccggaccgca taaatcgcaa aagtgctcac gaaaaccgac acgattataa agttgaaatt
     7561 gccaccgatt tgggcaccga ttatcaagca gatggtagca nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn tataaagttg aaattgccac cgatttgggc accgattatc
     7741 aagcagatgg tagcaatgcc gacgaaagct acacgactgt caaagagaat tgtcaatgac
     7801 atagccgcca gagtaatggg aatcgtatat tcggaaattt taaaatgatt gaccagtata
     7861 gccgcaaaaa ccgcctgcaa tacgaaaata atcccaatta aaaacacttt tttaggactg
     7921 gtgataatat cggtgcgata agcaaataaa aaagttataa aaaaggtaaa aataatcgca
     7981 accaccgcga cctggcagat aaatattgcc ggatcgccaa ttaccggtaa agctcgccgc
     8041 caactgcgtt gcacattggc taagcgcgct tgttctttag ccattgattc aattttctga
     8101 tagatttccg gcgtaatgcg cgtgttagcg tcaacgattt tttcgttttt aagaacaact
     8161 cccttgctga tggggacttt cgcaatggct tccgcttgcc gtttctcggt aatttgtttc
     8221 tggtaaatga ggttaggttt caggaaagcg accacgatgt cgtaagcggc attaaccact
     8281 tctggggtgt agtcatttag gcgggcttgc aaagccagtt tggcttttgt ccaggcttcg
     8341 gataattcta aaacctcgtt tggattttct aagacctctt cgccgccctg aaggatggca
     8401 attttttccg aaattatctg ggatttggga atatcgagaa tctgtaaaac ataaagctcg
     8461 ctaagcaccg attttacctg attctggaca tgagccgaga gctggttgac gttcttggga
     8521 tcgtactgat ataccaggga cttcagaact ggtgaatttt tgttaagctt ataatcgttg
     8581 agaaattttt tctgtaaagc aaaaaaagta attgaatcgg ccgtcaaaat tgccagcgat
     8641 tcttcgctcc gattaatact aatatttcgc agagattcat catacttgat tcgggctaat
     8701 cgtaagcgat caatttccgt aaaaaatttc tggaggttca ttagttcggt tttttcgacg
     8761 tctttatcct gaataaaaac aaacggaact tttttgatgg cttctaaacg atcggcttct
     8821 aattcctgtt ctgttttcaa gattgggaag tcgaacggcg caattattga tgagcgtgcg
     8881 atgtcattta ttttatactt agtgatgttg taatgagttt tgggaaagag taaagctaag
     8941 gctacgacga ttactccgca gattaaaaat acaccggtgc gctggcgcct ggattggaaa
     9001 acgccacgct ctttgccggc tattttagat gaattagaat tcgccacgat ttatgctcat
     9061 aatttaaaat atttactacc agcgtagagc ggcggaagcc caggtaaatc cagaaccgaa
     9121 tgcggctagg attagataat cacctttttt atatctgcct tcttgaagtg cgtcgtacat
     9181 tgccagagga atcgaagccg cagttgtatt gccgtattta tggatattac tatagacttg
     9241 gctcattgga atttccaggc gctgggcaac ggcttcggta atacgcaaat tagcctgatg
     9301 aggaataatt aaggtgatat cacttttatt cagattgttg gcttcgaggg cgatgttgat
     9361 tacttcagga aatttagtaa cggcatgttt aaaaacttga cggccgttca ttttaggata
     9421 gtgtttccct tcgtccagca tctctttgga aagacgcggt ctttttgaac cgtcgggagc
     9481 ttctacccag agttcattcg catagcggcc atcgccgaat aaatgagtag agagaattgc
     9541 gctttcatcg gtggaacgcg taataacagc cgcgccggct ccatcaccaa agaggacggc
     9601 gatatcacgc ccggcggtgg tcagatttag cccgacggac tggacttcaa ctccgacgag
     9661 gaggatattt ttatattgtc cggttttgat aaaagcgtcg gcaatcgcaa gtccatatat
     9721 aaaaccactg cactgcaccc gaatgtcgag tgtcggaata ttgggtaatt caagtaaagc
     9781 ctgtaaataa ctgctggtac ccggagtcag atgttccgga gtagttgtcg caacgataat
     9841 taaatcaatg tcatttttag ttaatccggc attggtaatc gcttccagta ccgccggtag
     9901 agccagctgg ctggcgccgc agggtggggt tacaaaacgt cgttcgcgaa taccggttcg
     9961 ttcgacgatc cattcgtcac tggtatccat cagtttttca aggtcagcat tagttacaat
    10021 attttcggga acatttttcc cgaggctggc aattttactt ttgtacattg atacaacttc
    10081 tcctgtcatt ttttaatggt agtcagaata tacgatatct ttgatattaa gttgaatttc
    10141 tttatagcca ttccattcat tttcatcaat tacaaaggcg atatcaaggg ggcgattgct
    10201 gattaacatt tcgtaatagc aacccatttt ccagccgata gcacttaaaa ccgaacggtt
    10261 ttgagccgct ttgaatttta agtgattttc actaattatg cgcggcattc cggagacggt
    10321 tacgttacgg gtgcagaata ccggtcgcat atttccgggc ccgaaaggag ccattttatg
    10381 gagaaaggag agtgtttggg gattaatgtc attaaagtca atttcgcttt caatttgcaa
    10441 tccggaatta agcatctgtt cgtcaatctg ttctgcggcg atttttttca agcgtctggt
    10501 gaattccggt agtgattcga tcaaaatggt cagcccggcc gccattttat gtccgccgta
    10561 attttccagc aagtcagcgc aggagttgaa ggcgctatat agatcaaagc cgggaatact
    10621 gcgggcggag cccttgccga ttccattctg gaacgagatc atgacaaccg gtcggttgta
    10681 taattccttt aattttgagg cgacgatacc gatgacacca tgatgccaat ttgtgttgct
    10741 tagaatcaga atcttgtcgg catctaaatc atgagtggcg ttaagttgta aaatagcgct
    10801 gtcgatcgtc tcacgttcga tagcttggcg attgcggttt tcctgttcaa ggaggttggc
    10861 tagctcaaat gcttctttac ggtcgaagga tgtcagcaag tgtactgctc gagaggcttc
    10921 cccgagtcgt ccggcggcat taagtctggg ggcaagaccg aagacgatat tgacaacgtt
    10981 cattgttttg ctatcgaagc cggaggtttt gagaagagct tgtaagccaa ctttttggga
    11041 actgttgaga tattccaaac ctttggcgac taaaacacgg ttttcgccga ggattggaac
    11101 gatgtcggcg gcagttccga ttgccactaa atcaagatga cgataaaggt tggtgactgg
    11161 tagattattt gcaatatgaa atccctgtaa tagtttgaaa gcgacgccaa ctccggataa
    11221 ttctttaaat ggataatggt catcttcgcg ctttggatcg agaatagcca aggcggcggg
    11281 gagcttttca gccggttcgt gatgatcggt aacgatcaca tcaatctggt tagaattagc
    11341 ataatcaatt gtatcgaagg cgttgattcc gcaatcacag gtgataatca aagttgctcc
    11401 gatttgtctg gcatattcga tgccgagttg ggagagacca tatccttccc gttctctgtc
    11461 gggtatataa aatgcggtgt caagccctat ttcgtggaaa accaggtaaa gaagtgaagt
    11521 agctgtggtg ccatccacgt cgtaatcgcc gaaaatcaag actttttcgc gtcgtagaat
    11581 ggcttgaccg agtcgttcaa cggctttggt catatccgcc attaaaaagg gatcatgggt
    11641 atcttgagta gaaggactaa aaaatttttc aattgaatcc ttatcaaaca gaccgcgatt
    11701 aagtaaaatc cgcacaatag ccggtggtat ctggagctca cctgccagtt ggtggacagc
    11761 gttgggattc ggattgctga taagccattt ttgtttcatg atataaaatt tctgaagttg
    11821 gtctataagc cgaattctgt atcccgacga atcgagacga cagccattta tctatccacg
    11881 acttttcagt cgggattaag cgagctaccc gggaatcaaa cggaacgggc tattcctttt
    11941 ccctgcttgc tcttgctccg ggtggggttt gcctggctcc cgcctttgca gacgggacgg
    12001 tgcgctctta ctttaatccc gacctcagtc gggccgcacc ttttcaccct tatcccgccg
    12061 gggcgggacg gtatattttc tgtggcactt tcctgcattc cgatcattcg gaatgatccc
    12121 ggattacggg acaccctgcc ctgcggagtt cggactttcc tctccgataa aatcggagcg
    12181 gctgtctgac caacttcaga aaagctcctc ttcataattg gaacgaattt cactgctttg
    12241 ctcatcccag tataaaatgc ggccgcagaa ttcacagcga ttaattcggt caccacttcg
    12301 tatttcaagg atcttctgtg gcggcactat tttcgaacat ccgccacagg aaacctgcat
    12361 tggaatatat tcaacatttc cctttttatc tactttttct tcaaaaatct gcagaattgg
    12421 gacaatcgca attccgtttt tggctttagc aatacgttcg tattctcgca tatagcggat
    12481 tgggattaaa tctacaatcc gcttcctttg ttccagtaaa gcctcctgag cgtctttagt
    12541 agtaatatca gttttgtcca ataaggactg tttttgatcc aattccttgg ttttttcctc
    12601 aagggttagt tctttacttt taatcgaaat cttcaggcgt tcgatttcat cggaaagttt
    12661 caagatgttg tattcggcat tgtcaatctc ttgcttgagg ttgtcaattt cggaagtcag
    12721 agcatcatat tctttattag aagtgactaa gtaaagttga tcttgatatc tatttaaaag
    12781 aatgcgatca tcggcaattt tactttctaa ttcttttatc tcccgcgtaa tttcttgttc
    12841 gcgcttacgt tcgttttcca gctctttgga taaggtatct atttgggctt ggagattctc
    12901 gacaatttgc ggcaaatcgc ctctaagatt attgatttcg agcaatttga tatcgatttt
    12961 ttgaacttca accaatcttt cgagttcttt aaacatttaa cctccagttg tttttataaa
    13021 aaaaatgcac cgcaagcggt gcattttata tggtgtaatg tattgtatcg gacaattaat
    13081 tctttcaaaa cccaacctat tacgtgggcg atatccgact tgaacggatg gcctccccga
    13141 tgtgaacggg acgctctgac caactgagct aatcgcccag ttaaattaac cggacatttt
    13201 aaaaaagcaa caaaatttag ctaatctgac tgattaatac aacagatttg caccaatatt
    13261 ttttcagttt attgaattat tatgtcggtc agtttaggta gtaaacctat caactaaaaa
    13321 gggcaaaaag agtaaccagg acaacaatcc aataaaaaag ggctgtatgt tgaaaagata
    13381 aatgggttcc attattgaac catttctcat tccaattatg aaccagatta atgttgcgaa
    13441 aaacgatccc agcatgccag tgaacataac ggatgacttg taattatact tcgtaaaatt
    13501 acaaataaca gggattaaca gagctgggat aaataatgtc ccaaaagtgt accagatttt
    13561 tactaccgaa ggaaggaaaa gaataaccaa tatagataag ccaatgataa caaagaatcc
    13621 gatttgggtg ttgcggttaa tattgctatt gggtttcaga cgccacaaaa ggtcccgtcc
    13681 aaaagtcata gctgagagca gggcaacaga atccaaagtg gacataatcg ttgccaacaa
    13741 accaataaaa aatatacctc gccaaaaatc tcccagtgcc atttcgccta atttggggaa
    13801 agccattaag ggatttgtat caggaatgag agcccgcgca tataaaccgc aggttgtcgt
    13861 gagcatatca aaaagcaacc agaaaatcac tgaaatcaat actccgcggc gcgctatctg
    13921 cggcgtgcga gcggcataac acttctgata aaaacccggg tcaacaaaag tccaaaaggc
    13981 gataaaaaac caaaccataa tatactgcca gttttcgcca ccagtgaatc tgaggtgatt
    14041 ttgcggcaga tttgcctgca ggaaattcaa cccgccatag ttagttatga gttttactgc
    14101 caacactcca aatcctaaaa acattagccc aaattgaaag aaatccgtca aaacatcaga
    14161 tttcattccg cccataaaaa catagaataa ggaaaaaata actccaatca gaaaagccaa
    14221 ccaaaaagaa attccgaaaa tagcgctaag taaaagcgta ctcatcaata aatgcgaagc
    14281 gggattagtc aacaaaaaaa caaacacact acccagaaat ccagccggtt tgccataacg
    14341 ctggtaaagc tgatcgggaa tcgtgtaaag accactacgt tgaatccgag gcgctagaaa
    14401 aattccaaaa agaatagcaa agaagtaata cggcaatccc tgagtcaccc aattcaataa
    14461 tccgtaacgg taagtgaatt caccgacccc caggatgccg ccataccagg ttgataccag
    14521 agtggcgacg aacgccggta atgaaagcct gcgcccggct aataaatagt cgtcaacatt
    14581 tcctcgataa ttccgggagt attttaatcc aattagaaat atgccgacca gataaatggc
    14641 gataattaac agatcaatcg gagccaatct aagagacgtc aatttagctc ctgaatcgcg
    14701 acaactaata aataaccact ggaaaattga atttgaacca tttcatcaat ggcggtatta
    14761 cttagaccgt gcgaaccacc tgttaaattt tcgtccttgg ttggatattg aaggccccga
    14821 gtagtcagat gataaactct tggaaaaccg aacagagaaa tggtctgacc ggggatggcg
    14881 ttaatgagcc agatgccttt tttcaataaa taaatttgcg ctgtctcggt atataacaga
    14941 aactgcgctc gccgggcata ctttctcatt atctgtaaag ttgcgatagt ttgatcatcg
    15001 cgccttccgg taaatccaac taatacgaat tgccgccagc catgtcgcaa aagccagcgc
    15061 aggcattttt ccaaatcatt gttattctga tttggttggt gaatgatctt tacctcgctg
    15121 gattgaaaat gatttatggt atccgtggta acagaatcca tatcgccaat tatcaaatcg
    15181 ggcattaaat tcaattggcg tgctttttcc gctccgccgt cggcgcaaac caatagcact
    15241 ttatattcca cttgaaattg tttgatatca aaccagtttg ggtaatttcc atttcccagg
    15301 actaaagccg tcggtgatag aaaatttatt ggtgtgttgg gagtcaattt aattcagaaa
    15361 attatttagg aaatcgagcg tatttttcaa ggtcattgct tttaagggcg gtatgtaatt
    15421 tatcaagatc gatttcgaag gagaaattag ctataagtcg gctaacctcc tgctgatatt
    15481 cgcgcccgcg aaagtattta atcaaatctt tggtgaaggg caggataagg tctcctgtct
    15541 tccaatccat tggtttgccg tcatttttat taataataca gttgaaatgc gccgcccaag
    15601 ctaccatcca gaatgagtat ttagccaata agtctttact gattgcatac atcaaagcct
    15661 gattataggg attgatgaac acgaactctt tactgaacat aaacgctgta tgaaagtaat
    15721 gcggtttatt gataatacct tccaagttaa ggcgacgggt cataagtatc ataatttcca
    15781 ttaggcgatc gccaatacca aggccgggat aattctggcc gggcaagggc ggtttttcgg
    15841 gtgaaaattt ttcgcgtgga ttttggagta gaagccattc tatatataat atattggcag
    15901 ttctacgggg gaaagacgat agtggcttat tttcaaaatg aagcggacct tgttttaata
    15961 ctaattcaca gattttattt tcgtttagcg gctttccgct ataggcataa aggcgatgga
    16021 tatacggatc actggtgtca atgtctattg taatattcgt caatcctttt cgctcgagaa
    16081 tatccaaaag tccgaaagcc cgaatggcat ttagcaaacc ctgtcggctg aaaacgccca
    16141 tgaaaaattg cgagtccaca gctaatctac catcactaac aatattatcg agacttaatt
    16201 cactgtttcc tattccggat tgaaggtcgg cgcttgtcag cttccgggca atttttgagt
    16261 attctttacg aaaacgggtc ataactttgc taatctaaaa aatttttact aaggaacaac
    16321 cttaattttt ccatcagcac cggactcaat tttgccgatt ttagccgcat ttattacacc
    16381 tgcttgatgt aattgtgcca gcatatcatt ggcaaattca tccgcaacag caattagcaa
    16441 gccaccagaa gtctgagcat cgcacaaaat tattttatcg ctatccgtta tcattttatc
    16501 ccagatgacc atcggtgaaa taaacgataa gttcgcctcg gtgccgcccg gaataacatt
    16561 gcgtaaaacc atctcgttag tggacggtaa aatcggcaca gacgaggctg aaatctgggc
    16621 tcggacacga ctgccggtgg tcatttcatg taaatgtccc aaaagaccaa atccggtaat
    16681 gtcagtgcag gcgtgcaccg gaaatgactt catcacttct gcggcggtgg cgttcagttc
    16741 ggtcatattg gcgatgatca gatttttcgt cagttcatct aataaaccgc gcttgagagc
    16801 cgtcgaaaga atcccgacgc cgatcggttt ggtcagaatt aaagcatcac cgggttgcgc
    16861 gccgatattt cggataattt tttcaggatg aataatgcca attgccacca gaccgaattt
    16921 tggttcgtta tcttcgacgg tatgaccgcc gagaatcggg ataccagcct gatcagcgat
    16981 aacctgtgcg ccgcgcaaaa tctgagtcaa aatttctacc ggcaggcgtt tttcagggaa
    17041 ggctactaca tttaaggcaa acagtggctc agcgcccatc gcataaatgt cactcaacga
    17101 gttagccgct gaaattcgcc cgaaatcgta cggatcatcc acaatcggag taaaaaaatc
    17161 aacggttgct acgatcgctt gttcgtcgtt aattttataa atggcggcgt cgtcggaagc
    17221 tgagaagtca actaacacgt taagtgccat gcgtggtctg atttcacgta gaattttttc
    17281 gagagtttgg ggacgaattt tgcaggcgca accgagaccg tgcgtgaatt gcgttaaccg
    17341 gattggttcg ttagatgtga ttgggataga aatttcctca gtgctagtta gacgatggta
    17401 agtttgactg attgccgata cagcatccgc gatttcatct tcggtcgtaa aacgtccgag
    17461 cgagaagcgg acacttccga gaatccgttc attcggcaag ccaagcgctt ttaaaacatg
    17521 cgaaggcgta atgctactgg catggcaggc ggcgccagcc gaagccgcaa cttgcgtcaa
    17581 ttccgccagc atggtattgg catctattcc cggaaaataa acattgaaag tattggcgag
    17641 tttttgcgtt ggatgaccgt ttaagccaag ttcgggaatt gcagttcgta aatcgttgaa
    17701 gaatttttca ccaagcgcct tttgtcggga cacaatttca gccgatttcg ctcggaatat
    17761 ttcagccgct ttacccaagc cgacgatttc cagcacattt tcggtgccgg gtcgcaaacc
    17821 acgttcatgt ccggcgccat aaatcaatgg agcgatttga gtgccggttc gtatgaaaag
    17881 ggcaccgaca cctttgggag cgtacatttt atgtccagcg actgagagta aatccacatc
    17941 cagctcgcgc acatcaacga ctattttccc gaccgcctga gcggcatcgg tatggactaa
    18001 aattccgtgt ttatgggcga gagcggcgat ttcgcgaatc ggctgaatcg taccgatttc
    18061 gttattggca agcataatag taattaggaa agtgtcggga cgaatggcgg cttgaacggc
    18121 aggcaattcg accaagccat cactattgac cggcaagtaa gtaacggtat aatttttact
    18181 ttccaggtag cggcagactt ccaggactgc tgggtgttca atagtgctgg taatgatgtt
    18241 gccgccgggg tgcatttcgg ctattccgcg aatagcgaag ttattggatt cactgccgcc
    18301 gctggtgaag acgatttctt caggctggca attcaacatt tccgcgacct gacggcgagc
    18361 attttcgatt gcctgtttgc tttcctgtcc gtagaaatga ctgctggatg gattgccgaa
    18421 gacatttttc agaaatggta acatagcgtc caccacttcc ggtaggagag gggtgctagc
    18481 gttataatca agataaatcg gtttcatcgg agcaaaatta attatggttg atatagaaat
    18541 aaagactaaa acggattaaa atgggaaaaa tgataggcga accacaataa aatcgtcgta
    18601 ccgggctgtt ttgaattaga atttattaat cagcagactg aagtcggcac tttttactga
    18661 gtgggtcaat gccccaaccg agataacgtc cacgccgctg gcggcgtaat cacgaattgt
    18721 ctcgagtgtt atattgccgg atgcctcgaa gaaaattgtt ggataaaccc cattattttt
    18781 cgttgcctta atcagttgcg cgatattttc cggcgcaaaa tgatcgaaca ttatcacgca
    18841 gtaggtttct ggccttaagc ggttataagc ctcgacaact tgccagaatt catcgtcgtt
    18901 agtgacctcg acttcgacga atcgcaaaaa cgggtcagtg gctacaatag cgtctaaggc
    18961 agtcataagt gcttgcggat cgccggaatt ttttaaaact gttaagtgat tttcttttaa
    19021 gatggcggcg tctgccagtc cgagtcggtg agttaatccg ccgccgtatt gcagtgcgct
    19081 tttatctacg attccccaaa gagttttacg ggtggcagtt acaaaacagg gcagaccttt
    19141 gagcgcctgg ctatattcgg ctgtaacggt ggcaattccg gacaatcgtt ggaaaatgtt
    19201 taagacggtg cgctcgaccg tcagaatgcg atcagcccgg ccttcgatag tcataatcgt
    19261 ttcgccgcct ttgaccggat cgccatcttt aaaatctgag gtgcttagta gattatgatg
    19321 atcgagaaaa gcctgtattt ccgccattcc ggcaataatt gccggcgctt tggcattgat
    19381 aacggcggtt acctggcgcc gataatattt cgctggttgt aaagatgcgt ccttactgct
    19441 gatgtcaaaa tccagcagat tgtcgaaaaa agtaagtgcg aatttacgat agtatttatt
    19501 ttcgagtcgc aattcggtcg agatgtcgaa gcattgcttg acatctttag tgcgtgaatc
    19561 tgtgaaattt ttgacggaat tattcataga ataaatcccg aacgttgacg attttaatat
    19621 tttcgggtcg gatagcgagt gggtagtttg caatctcgag gtcttcttta agcacttcct
    19681 cagtcttcgg agtaatatat ttaatcgggc taggtgtatt agcgagcagg tcttcgcgca
    19741 atttttcggc ttcgttggtg acgattgcga tttgcagatt atcatattgc agatatttgc
    19801 ggatggcgct attgacgtcg ttaacggtca tattgtcgag tgcgatgcag aaattttcca
    19861 gatgacttcc ttttattcca tagaatttgt cgtcaattgc atagccgagg cgcatcattg
    19921 tcgtcggggc ataatgcaga atatacttgc gtaggaaatt gcgggttaga ttgaattgct
    19981 ccgccgttag tccgttttcg atgaaattat gtaattcacg catagtcgct cttaggacga
    20041 aatggcgggt ttcgttcggt accggccgaa tccaaatttc gaatatttgc tgatggcggg
    20101 cgacgttgac cggtggaaat tggtaatagc cgctattggg aaaatgttcg atatagctgt
    20161 aatcgccata attcaagccg cgggtttcgc ggatgacttg gtagagatga ctactggaat
    20221 tgcggtgctc gccgaaccag gaattggcga ctgctagggc ataccagtcc ggattttccc
    20281 gtgtgatatt gattggaaaa ccaagactaa tggcagtagc attagcgtcc ttttcgacga
    20341 tggtaacttt taagccctga atgtcgtagg gaacggctcg agttgcgata ttttccacgc
    20401 cgcgaggaag ttgcgccagg tctttctgca attttgccaa tgtttcgtta tcatatccgc
    20461 cacctacacc aataataaca ttgttacggt tatacatttt ttgatagaaa tctttgacgt
    20521 cgttgatcgt aatggaatta atgctgttga ttaatccctc ggtcggatgt ccgtagccgg
    20581 ttccggcgaa gatttcatta taaagcaccg ccttgccgag ttcttcgtca ttagcgtatt
    20641 tcaaaacatt ttcaagataa ttcaagacgt tgcttttcag acgattcagg tcatcttctt
    20701 ggaaagccgg tttgaggatc gcttcggtaa aaagcgggta ataagccggg agattgtctc
    20761 ggtgcacgcg gccggaaatc acggtcatct ctgccgaaac cgtagcgtcg taggacgccg
    20821 ctagtggaaa taatttcgca attatttctt cgtaagaatt actttgagta gcggcttcac
    20881 ttaacatatg agcagtcagg aacgccaatc cctctttacc ggcgggatca tcctgggagc
    20941 cgactttgaa ataaatgcgg taagagacag tcggatcttc ggctaccggt aggagcacac
    21001 aacgagaagc gtcgatttta gaaccacaat ttgaaagtaa aatgctagcc ataattcccc
    21061 aacttaatag tagagttaat ttggttttca ttgcgcgact cctttaagaa tgactactgt
    21121 tcttttttcg ggaacgccat aatactgagc ggcttgctga atatcggtga cggtgacatt
    21181 cattatttgg ttaaatagtt gatcaacggt ttcaactcca cctgtaatgg caacgtaatt
    21241 agctagattg cccgccacgt tatcaggagt gtccagattc atcagaaagc tgtatttcaa
    21301 atgttttttg agatcctcga ggcgagactc agggacggct tcagtcttga attgttccat
    21361 tgtcataaaa atctgttcgc ggacgtaatc aatatcgtcc ggcgatttta ccatagcgta
    21421 aatttcgttc aatttaggat cgcggctgac cgaaaagtcg gctgtgatga actcgacttt
    21481 ctgttctttt aaaaccaatc gcttgtaaag gtcgctattt tctccgaaag ctaattgctc
    21541 tagcagaaga ccggctacat atagacgatt attaggatca taggcatcac ttttgtaagc
    21601 gaccatgacg attggcaggg ttttcccggg ataaattact tccgccttgc gctcggcagt
    21661 ttgaggcggt tcgggttcga tttggggaga gacatagccg ggttgccaat cgccgtaata
    21721 tttacgaaca agttcgagag ttttctcggt ctcgatctga ccgacgatga gcagaacgac
    21781 attctccggt cggtagtaac gttggaaaaa agttttactg tatttataca ttgtaggcat
    21841 agctttcaca tcagcttcaa atccaatggt agtgtgtttg taggtgtgct tgtcgaaagc
    21901 gacatcgtaa agcttttcgt ataagacgaa ccacggactg actcgccctt tgagaaattc
    21961 accgtaaacg gctccggttt cggtcttaaa ttcgtcctcc ttataagcta aattgatgaa
    22021 gcgatcgctt tccatctcga tgattttttc caggtcttct gcggcaaagt tcagatgata
    22081 acaggtgtaa tcatcggtgg tataagcatt agcgtcggcg cctaattcag ttatagtttt
    22141 atcataaagc gagccgggat atttctcagt gccgcggaac atcagatgtt caaacaaatg
    22201 ggcgaatccg gaatgtcccg gttcccattc gtcccgactg ccggtgcggg tgatggaata
    22261 ataagccacg atcccgttgc tttccattgg aatagtaatg actttcaagc cgtttggtaa
    22321 aacttccaca ttatatttat aaggaaaaaa cggctcagcc gtctgaccgg ttacccacag
    22381 gctgaacatt aaaattattc ccagacttat ttcttttaac ataattttct ccgctatctt
    22441 aaaactcagt taatttaaaa aaagtataga aattaaaagg agaattgttg cagttttttt
    22501 cgagacgagg caaatgctaa cgctccttgt tagctaagtt ttatttctat ttcagctact
    22561 caactgtgac cgattttgcc agattacgag gtttatcaac gtttaagcct ttcagatcag
    22621 cgatgtggta ggctagtaat tggagcggta tgacattgat gagcggcgaa aggtcttcgc
    22681 tggtctccgg cacaattatc agatgattgg agagttcttg cagatgtgga ttattatggt
    22741 cggtgatggt taaaatttgc ccgccgcggg ctttgacttc ctgcatatta ctgagaactt
    22801 tatcgaaaac agcccccttc gtagcgataa aaacgactgg catattttcg tcaatcaaag
    22861 caattggtcc atgtttcatt tcggcggctg ggtaaccttc ggcatgaatg tatgaaattt
    22921 ctttgagttt caatgcgcct tccaaagcga cgggataatt gataccacgt ccaagataga
    22981 taaaattgcg attcagataa taagccgatg caatttcccg cagattgtca gaatgttcgg
    23041 ctacctggcg gatttgttcc ggaatcatat ctatcttggc taaaagagcc ggatcaatga
    23101 gttctccttt ttgttgcttg atatagagtg ccagtagaag taaggcaatc acctggcaaa
    23161 taaaagcttt ggtactggcg acgccgattt ccggtccggc gtgtaagtaa atgccggaat
    23221 ttacttcgcg ggcgatagta gaaccgacta cgttgataat gccaagtgtc tgcgtacctg
    23281 ctgatttggc ttttcgtaaa gccgccaaag tatcggcggt ttcgcccgat tgagaaatga
    23341 cgatcatcag aacttcgccg ttaatgatag actcgcggta acgaaactcg gatgcataat
    23401 ctacctcagt cggtatgccg gtatattttt cgattaaatt cttaccaatc aaggctgaat
    23461 gccagctagt gccgcaagcc gcaataatga tcctttgcac gcgattaaaa tcagtgttga
    23521 gagataattt gatttgtccg ttagtgatgc gaccgcgcaa agtgttctga acggcttccg
    23581 gttgctcaaa tatttctttc agcatgaaat gtttaaagcc gcgtttttcg atctgagaaa
    23641 tattccagcg gatttcttgg atctcgttgg ctatggactg accgtcgagg ttttgcaaac
    23701 gatagttgtt atatccaatc acggcgattt cattgtcatt caagtagatg acttttttag
    23761 tatgaggaat gatagaaacg gcgtcggaag ctacaatagt ttcctgatta cctaaaccga
    23821 taataatcgg tgagccacgg cgagcgatta ccaaatgttc ctcatcttta tgcatcactg
    23881 ctattccgaa agtaccctcg acattatgca aggctttaac gacggctgtt gctaggtcgc
    23941 cttcataaaa tttttcaatt aaatgagcca atacttctga atcagtttcg gacgagaaat
    24001 gatgcccttc ttggattaac atatctctta gactacgaaa attttcaatt ataccgttgt
    24061 ggactatggc aatttcacca tggcaggaga ggtggggatg agcgttgatt tcattaggag
    24121 cgccatgagt tgcccaacgg gtatgggcga taccgatatg ggaagaaaac gaatcgagag
    24181 cggccgtttt ttccagttcg gcaattttgc cgctcctttt aaccacttcc aggcgatggt
    24241 cgccagcgat gcaaattcca gcgctgtcgt acccgcgata ctcgagtttt ttaagtccct
    24301 ccaataataa cggaacggct gattgattac cgatataagc cacaatgcca cacataaatg
    24361 aatcctttcg aaaaagtcat aaaatcgagt tgtaaaatgg ataggtataa aagaaatgac
    24421 tatctaatta tggtcagagg ttcctcaatt ggaatatatt ttgacatagt cggatttggg
    24481 aaaattttaa tatccataaa ttctaattta ggcagatgat attattcact ttaaacggtt
    24541 cttttgcatt agtttataaa gatattttca cacagcgtta aaataggcaa tggagaagat
    24601 ttctgcaaaa aaaagaacaa ctttttaatg ataggaattc gaaaattcta aaatttttat
    24661 atactgctta tgccaattca aa
//
LOCUS       DAOOMU010000025        23986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_1989_length_23986_cov_17.751243, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000025 DAOOMU010000000
VERSION     DAOOMU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 23986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23986
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1989_length_23986_cov_17.751243"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="PLI55_03765"
     CDS             complement(<1..1062)
                     /locus_tag="PLI55_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPA99985.1"
                     /translation="MRTTIQLFALMLFIFSIGKICQADTTEVKQQIETIIAEIRKKYC
                     PDQRVALWKIEIDYQDNDYNLVGETDHPEAVRALTNQIKQTLPAIKIQNLIKILPDET
                     VNGKNFGIVINSVEPLRSGPSVFNDMVTQTLRGLTVEILKSAEGCFLVKTDDGYLGWM
                     EDDRLVVGGDSLKTAWENASKVVYDDFEGIIYSKADRKSMPVADVVLGNRFKMIDKQG
                     QWTRVEIPDGRRGFIPSQELVSESEYLARTPTAEGIITTAKNLLGRPYLWGANSPKAF
                     DCSGFTQTVYRRNGYLLARDANMQVNQGFAVDTTNLLENLMPGDLLFFGPNPEQINHV
                     AIYIGDYEYIHCSAWVRINS"
     gene            complement(1071..2717)
                     /locus_tag="PLI55_03770"
     CDS             complement(1071..2717)
                     /locus_tag="PLI55_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPA99986.1"
                     /translation="MKLSFKKGLKIWIAVLVSVSVLLAFTAKDIYKTISDSYKLYNAV
                     FQQILLNYADPLDAAALTESNIRNMTRELDPYTVYMTEEEKEPLEILSKGEYGGVGLR
                     ISLRNDTLTVISPIEGSPAKKANILPGDQILKIDTIWTTGLDLDKCTRLIRGNVGTKV
                     ELVIRRPGADEGKVYILERAKIQVYDVYYADYLEEGIGYIRLAEFSKGSTAEVRQAIE
                     NFKDHDRLEALILDLRGNPGGLLTEALTVAELFTQPGDTLLFTRGQNESSNRVDVARR
                     RPLLDESVKIAVLIDGGSASASEIVAGIIQDLDRGIVLGTPSFGKGLVQTVFRVDKEH
                     SLKITTAKYYLPSGRLIQKPDYIKNPKLIAASPSQDSIFYSRHKRQLKSKGGITPDII
                     IKSDEQSDFITELWRQNQFYSFAVKYKTEHNELPEEVNERIIKEFKNYIDHSGFQFIE
                     KTEKNIREMDKIISQDERLKNVKIDFQPVYEIFDSLKEAEFENNHLMIQRGIENQFAM
                     LRDGIAGQLKADLKYDKVVSEAILAVRDDGKYKTTLGLAR"
     gene            complement(2714..3346)
                     /gene="tmk"
                     /locus_tag="PLI55_03775"
     CDS             complement(2714..3346)
                     /gene="tmk"
                     /locus_tag="PLI55_03775"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPA99987.1"
                     /translation="MTKGLLITFEGIDGSGKTTQIKYFIKKCERENIPVALFREPGGT
                     PIGEKIREILLSKSNSEMFAITELLLYSASRHQLCRELIRPALESGKIVVCDRFYDST
                     TAYQGYGRGIDLNFINMLNRIAADNLVPDLTFIFDIPVAERLQRLGTRDLDRLEREEH
                     NFQEKIRQGFLKIAAAEPNRVCLIDGTKTEELVADEVWHKFNKIIRKMQL"
     gene            complement(3391..3801)
                     /locus_tag="PLI55_03780"
     CDS             complement(3391..3801)
                     /locus_tag="PLI55_03780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPA99988.1"
                     /translation="MLEHHYQLRVRYSSVDQLGVVYYSRYLEYFEAARDEIMRAIGLP
                     YSQFEQAGYAMPVVEAHCVYHRSAHFDDLLTIISRISAMPTARLRIDYEILRENESQP
                     LVSGYTIHAFVNQSGKAVRPPQFFLKLLAAQMSK"
     gene            complement(3823..4803)
                     /locus_tag="PLI55_03785"
     CDS             complement(3823..4803)
                     /locus_tag="PLI55_03785"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HPA99989.1"
                     /translation="MAEIVLDFEKPIIELEKKIEELRAQSAASGVDLNPEIEKAKLKL
                     NRLIQNTYSNLTRWQRVQLARHPERPYTLDYVKRICSEFIEIHGDRYYADDKAVVAGL
                     AQIEDYKVILIGQQKGRTTKENLYRNFGMPHPEGYRKARRAMKLAEKFKRPVISFIDT
                     QGAFPGVGAEERGQAEAIARNLFEMARLRTPIIVVVIGEGASGGALGIGVGDRILLLE
                     NTWYSVITPEGCASILYRDSSKAPLAAEAMKVTARDLAEFKIADEIIPEPLGGAHRNP
                     DQMAQTLKDVLLRYLAELTQIPIDELVDQRIEKYARMGSWKVVDVKSKSK"
     gene            complement(4811..6943)
                     /locus_tag="PLI55_03790"
     CDS             complement(4811..6943)
                     /locus_tag="PLI55_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPA99990.1"
                     /translation="MGSCIYGLDTEITADFQIFVNMQISVIIVSYNVKEFLQQCLLSL
                     KNALEGLSSEIIVVDNDSVDGTVNVIKNKFPEIILIENHFNKGFAAACNQGLAIARGE
                     YILLLNPDTMLQEDTIKVMIEFFRAHPEAGAAGCKILNADGSLQLACRRSFPTLTVAL
                     PKLLGLSRLFPKVKAFSRYNLTYLDPDQITEVDAVSGSFLMMRRAVYEQIGGLDESYF
                     LYGEDLDYCYRIKKSGWKIYYVPDTKIIHYKGESAKHAAFDNFIAFYRAMAIFASKHL
                     GKSSSIITDIVFKVGIFFRGIISLVAKFFRRRMVMIVDGIALVAAILVAHQLQPRPLP
                     DYPSLLSMLVFYLLVWLGIGYSIGLYDRRELSYSRAVVASLLSFVASLIFNLIFKDVI
                     YSPNMLLLSFIIVTLFLPGWRVLLLFLQRRRVIQPASSLSKALLSRRTIIVGAGKEGE
                     RIARKLKTHIEHGFEILGFVDKDYMNEEIAGFPFLGLVSDLPEIIRINHATEIIFTTD
                     RFNNDDILGIVDKIKKYRTNIKIVPRNLDYILGKSSVENIEDIPLVNIDYNLFHVGNR
                     VTKRIFDLLISSISIILLSPFVIPIAFLLGYRFQKANFIGAEQHQISALTLVKRNGNH
                     VHPFLNRFPLLFSVVKGDISLVGSELVPAENGERNLRFKPGLTSLFRVSKNHRTDEND
                     KQNYEHFYMQNHSLFLDVEIILKTVLKI"
     gene            complement(6883..7599)
                     /locus_tag="PLI55_03795"
     CDS             complement(6883..7599)
                     /locus_tag="PLI55_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPA99991.1"
                     /translation="MKSLVIIPTYNEKDNIRTVIERLQALPIELDILIIDDNSPDGTA
                     DIVRELQADDPHIFMINRPAKLGLGTAYITGFRWALERDYEYILEMDADLSHNPDDVP
                     RLIQECENGYDLVIGSRYCNGVNVINWPIKRLLLSYGANKYTRMITRMPIMDATAGFK
                     CYRREALAAINLDRVKSSGYSFQIEMHFRVWDAGFKIKEIPIIFIERSEGLSKMSKNI
                     VWEAVFMVWILKLRQIFRSL"
     gene            complement(7568..8479)
                     /gene="rfbD"
                     /locus_tag="PLI55_03800"
     CDS             complement(7568..8479)
                     /gene="rfbD"
                     /locus_tag="PLI55_03800"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPA99992.1"
                     /translation="MKILITGANGMLGEKCTRLLATKHRVLATDLHDKLIYKLETDYR
                     KMDITKPEEIENIIGEFQPDWVINCAAYTNVDGAETDRETAWQVNAVGPMNLVAALRK
                     SNTPIIQISTDYIFDGVNGPYRENDPVKPINYYGETKLAGERAVLESGLPATVFRTNV
                     LFGSSTNQEASFVYWVVNKLSHREPINVVNDQFGNPTWADGLAQVIEMAIERGINGLY
                     HYGGADYINRLEFALQIAAVFELDPTLIRAVSTRSLNQKAPRPFRAGLVCEKIQSEFG
                     IHLYSVKEALTNMKEKQFEVISNHTDI"
     gene            complement(8482..8943)
                     /gene="purE"
                     /locus_tag="PLI55_03805"
     CDS             complement(8482..8943)
                     /gene="purE"
                     /locus_tag="PLI55_03805"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPA99993.1"
                     /translation="MEKIALIVGSNSDLETVEQARKYLEYFELDCEIKVLSAHRQHDE
                     LVNFVKQFEKENGRLIIACAGMAAHLPGIIAALTTLPVIGVPLNASSLNGVDALYSIV
                     QMPGGIPVGTMAIGKPGIINAIIYAARILALSNAKIKAKLVEFAAAGFKLN"
     gene            complement(9019..10038)
                     /locus_tag="PLI55_03810"
     CDS             complement(9019..10038)
                     /locus_tag="PLI55_03810"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HPA99994.1"
                     /translation="MNKKIGLLVLIISGMLMNCNQLRESIGGVDDIYIFATDQTREVL
                     GKAIDTTFSYGIRTPEFQPYFSTKWLPVEEFPSYIKFKNIILVADLTQEDLAAEIARG
                     ILPKQSLIAAEKDSIHMFAIPDNWARDQMFVLIAGRNLKMVERSILEQKGWLYGKFEE
                     KFEEVQMDYIYGRYEQKKLTNYLWQKYRWTMRIPHDYIIIRELPENNFVWLGRGLPYR
                     WISVTWGDGIQTEWLTNVGLYEKRNEIGKLYGGIMTDKRFLGFQFFKFGEYDALKMYG
                     LWYHTEETQGGPFETYAFYDWRTDRTFIIDMLMYGPGEKLSVLFRGLELTAKTFTTDY
                     KGSWK"
     gene            complement(10109..12253)
                     /gene="rnr"
                     /locus_tag="PLI55_03815"
     CDS             complement(10109..12253)
                     /gene="rnr"
                     /locus_tag="PLI55_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPA99995.1"
                     /translation="MGKHKNNSSKPKSVKVRTDTQIVERIIQFLQRNKGNIYKQKKIA
                     REVGISSNKYVRFKQILKELAIEGEIENPRSGWYRIPDKARFVEGIISFSGRGFAFVN
                     TDAGEEIFVGADHTANALHHDRVLIEKLRYSNGIRPEGKVVKVLNRGQKSIIGTMKRK
                     NKHWVVIPEHPAAVEEIELVGSEKGLREGQLVEIQDIEWSNARLRPRATLKQILGFPE
                     NPQDDLIIVKKMYRLSDRFPTKAWQEAERLGNQKIKADNRLDLRDKFIFTIDPPDAKD
                     YDDAVSLEKSPDGNWLLGVHIADVSHYVTPGSVLDREARRRGTSIYLGENVIPMLPPP
                     ISDELCSLKPGEDRLAFSVILTLNGAGAVVRYSFAPSIIRSFRRFSYEEVQEILNTKS
                     GEYFDMFTNMRQISRTLFERRRAAGSIDFDIPEPVFKMGPAGIPIEINPSERLDSHRI
                     IEEFMLAANRAVAEYISIKRRNEKLPFLYRIHPQPPQQNITELYQILRNLGLDLHQPP
                     KLKPMDIQKILNGLTEMPYKNFIEQITLRSMAKAIYSHQPLSHFGLAFPHYTHFTAPI
                     RRYPDLVVHRLLKNYLGDYDSNDLAFYRRSLPHIASSASQREIEAMEAERTYQKIKQV
                     RFMADKIGQWYKGVISGVREFGFFVEISQFLVEGLVHIRTLSDDYYIYDPENHLLRGK
                     YSQRIFRLGDEVMVMVTDVSVRERRIDLEWGE"
     gene            complement(12253..13779)
                     /locus_tag="PLI55_03820"
     CDS             complement(12253..13779)
                     /locus_tag="PLI55_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPA99996.1"
                     /translation="MTDQRHRRKNLQIVIFLIIGFVGFLPLLGIAQDTSRAEVIFGEI
                     TVRALEEKLTDFTEIARDYTNYPVTADSYEELTNKILDAVLKDGYLYPVLTLSAVQPE
                     TEGNLTKLNPEFRLDRGERVVIDTVIYEGVKRTAPELLKRESAAILGKSPDSQKISAF
                     LNNFKSYSFLTVQSQPEIVKTREGQFGLVVQLTEHSTNTFSGIVGYIPATATTKGYFT
                     GEMNLNLNNISGRGRSLSVFWAKSNRYSQQIDLRYFEPWIRHTNFNAEGQFSQILRDT
                     LVVLRSFEAGIGRRLFQNGSGALKFNYTSTMPTSGGRQLLGLNDLTTRGLGLSFRYDG
                     RDYPLNPQKGIMVAINAFLSRRIEQNVGRLWQYQTEINGELDVKIRSQLVAACGWNLR
                     GKWLSEGVPVYSDYYWFGGSRSLRGYATELFGGSEVAWLNNEIRWLIGKNGRAHIFFD
                     QGYYRRSVEGLDKKYFPHSYGVGLRLDSRMGIIGLDYGFGEGDTFTTAKIHIFLESKF
                     "
     gene            complement(13779..17042)
                     /locus_tag="PLI55_03825"
     CDS             complement(13779..17042)
                     /locus_tag="PLI55_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99997.1"
                     /translation="MDTLKIAASDSVVYLSHRFVIPETIELNVTDSISLDPLTGAITL
                     HSTRTDSAFFIAKYQYLPLNLPLNRIVNLPPRIYTGNEIAEPSSTDYPAHPPAQYSTE
                     TTDFLKSGTLYRGVTLGSQSGMSLQSGLNLELQGKIAEDIAIVGTITDQNIPIEPEGN
                     TQTLDEIDKVFITVVLPHERLTFGDYEMAYSSGSLGNYNRKLQGVYLESERPPMRNVM
                     SGAVTKGQYYHNYFLGEESNQGPYQLIGRNGETDIIVLAGTEKVWLDGRLLIRGESND
                     YIIDYSTAEITFMTRQIITAESRISVDFQYSDLIYQKNIYMAGNTTNLFDEKLRVSAA
                     AITEKDDRENPIELVLSGADKKLLKSLGDNAQDAYISTITSDTNGSYILVDSILIYAG
                     PGQGTHTATFYNVGTAGSYKKVYRGEVTYFEWVDKNNPLTAASEIASALYLPVKPLKL
                     PTSRTLYHLASDWRPHQNFAIKAEVARSDFDQNLFSPLDDEDNAGLAVDLTSNLIIPL
                     SESSKIALAGHYKQENWGFEPIDRHQIVEYRRKWDLPSDSTNGEQYYEGQIQYALKNN
                     LVVKTEGGQYSRGDFNSNRFAASAGLSYKMLENVQISEEFIQRNQTGFNDINWTRRGF
                     RLITHLWKVKPFATLNYELREGDTIRTENFRFLEQIYGIGSASPGRLTWQVQTTLRRD
                     DLSDSNTWIKGTNARNIGLNGQLINWHSFSSQWNYIYRIKKFYNQNNSDIVVHLLDFS
                     LKQEPRKLPYRWETTLKIENEQTVKKEWRYYYVGDGMGDYIYDSTYADYVPDPQGDYI
                     LRILPSSIKVPITSIEDGLRFQFDGANVKMPIFGDWLKRISTLTDVRLRQEIHDPQGI
                     IKYLATDINQVDTSWANYSRIIQQDVNLRSRERKSDLRLRFYSSDLITQADVRGLEKT
                     FTNEWSLRYRNRYLGKTTIESESALKNYSRQSEINSLRGRDIYTVREKLTMSTFLDRI
                     HLIGTEITLNSDKAKGSDPIESLLAGVKLSYERKIVGKGRWKSFVELDKVSVTPKGKA
                     IPYEMSNGKKEGLTGGWGTTVEYRVGSNVSIRVNYEGWKEPERSVYHLGSGEVRLSF"
     gene            complement(17185..18087)
                     /locus_tag="PLI55_03830"
     CDS             complement(17185..18087)
                     /locus_tag="PLI55_03830"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPA99998.1"
                     /translation="MKRSSKISLLIFILIWNCTIKAQFRFEREIVPQSVREGVQFNAI
                     SVNEFDDIYLLESKFSEVYRLDQNGNILNRNGGFGWGLGQFDCPRDLCFSGLDVIVAD
                     QNNHRLVRYDRQLNYIATQDLRSDSRRLIYPMSLTASQINELFILSGETAEILRLYVE
                     KNEQTWFGGIEYGVYALVHPVSLRINRKGILTVLQEDGSLVQFDRFGTPLGLIPFHNS
                     KINKCKAYGLTAVNNDWLILIDQTPFLRMFYSKSKIWDEPTLADFDRLEPFIATTFSN
                     NRLYLLTSNCTILVFSMETSQSKP"
     gene            complement(18371..18835)
                     /gene="tnpA"
                     /locus_tag="PLI55_03835"
     CDS             complement(18371..18835)
                     /gene="tnpA"
                     /locus_tag="PLI55_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HPA99999.1"
                     /translation="MKSGVYTQIYLQLVFAVKNRDAVLNSDMLKRVFEYMSGINTKLG
                     HKSIIVNGHYDHAHLFIGLNPNISISDTMHDIKRNTSIFINKNKLCYGHFNWQEGYGA
                     FSYGRSQINDVFRYIQNQEAHHSKRTFREEYIEFLKKYGIRFDSQYLFDFFD"
     gene            complement(19134..20366)
                     /locus_tag="PLI55_03840"
     CDS             complement(19134..20366)
                     /locus_tag="PLI55_03840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPB00001.1"
                     /translation="MKRFILIILVVSVLIVTVFGEESVVIKRENTHFRQGPGSYYPLI
                     GMLQKGVQVAVVGTQSGWMNIQCAGQIGWISENAVIASDEPTGSVLTGSISGNGSMMI
                     SRASASGAVKGFAQKFVNFHDGDPAFIEQYDVELFTPAEFQRFRQETFRGRDPEKIRK
                     RYKRIKSENTDHSVSLQEEKIGLAAASRIAASGLVANQSQLKYINLVGNIVLENTDMY
                     DYPFKFYILKDSHPSAYAIPNGMIFVTSGLLELLQDEAELAVILAHEISHVVQKHGAE
                     EIAKRKTMIVADEGFNELDQEIVTEDATADELDLIALNCYETATKRRQIEYEIEADQL
                     GMIYAYRSGYDPSALGRVLTRIEANTSPDFWNPESNWSYDAVFDRAERVNTFIKKNLN
                     KNPEWNVTNPSRFRSVFK"
     assembly_gap    20432..20531
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<20532..22436)
                     /locus_tag="PLI55_03845"
     CDS             complement(<20532..22436)
                     /locus_tag="PLI55_03845"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB00002.1"
                     /translation="MKINTKKILIGLGIALIITLLSGLLYHNGLIQRWENQVNDILYR
                     DFQVEQHPSEAVIIAIDQNSLDYFQKNFQILWPWPRDIYAMATNYLNSCGAKVIVYDI
                     IFSTPDIDRLNIQAEYADSAFARAMLSSGKVILAMQMEDSTHLSVEKLPTQFNRQIVY
                     NVPQRIVYKYPNVTAPIERFQKAMAYPGAVNFFTDNDGVCRRVPLFFAKDHQIVPYMA
                     LAAVMIYDDISSIDYDSMRHTIFAGKRRVPLNNSGFFDIYWYGPGGAGNTFQYVSFAQ
                     LLNSYLQVQNGQKPDLDPAIFQDKAVFIGATAAGLLDLKTTPFSSVEPYPGVEIYATI
                     FSNIIRGDYISNLPFIIWLGFSLILIWLLAFLWQKLKIWQSAGISLIILFIPLIMSVM
                     IFQRLKMFMPVVTTEIAFVLSITVVLLVNYLTEGREKKMIKKVFNRYLHPTVVEVLTR
                     NPERLEMGGKEIVATVMFSDLQNFTGIAELFTPPEIVGFLNQYFTKVEQIIFKNDGML
                     DKYTGDGIMAIFGAPIPTNEHARMACNAALDFKNLSTFTIEVKNRTIPLITRLGINSG
                     NFVVGNIGSANRMDYTAIGDTVNLAARLEGVNKIYGTQNIISETTYSLVKENFICREL
                     DLIRVKGREEP"
     gene            complement(22458..23903)
                     /locus_tag="PLI55_03850"
     CDS             complement(22458..23903)
                     /locus_tag="PLI55_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPB00003.1"
                     /translation="MIKNIHRIIGLLCLIIWIDCEGSDCRALIENGEFKKAEEIIRRE
                     LFSNPSLSVSERRTLEFELERMKRIRLDFNKSQAEVIEYIKQYVPDVTPTDLVRWESE
                     KSLEYLVIDGEKMYFKDAAPNLFRLDRTLKAVKQKVDSQIPTDSRREPAFDLDSHCRK
                     IVQAVQTTQKSTVLPVRMKIKQSLTVHPNVVPEGKIIRCWIPFPREIPDRQVNIRLLK
                     TDPPIYILANTDQTLQRTIYFEKAAVKDSTTHFSVEYTYEIYGSYTPVKAEKVIPVEP
                     NPDLKPFLSEEPPHIVFTDEIRQLSQRIVGSETNPYRIAQKLFEWIDVNIVWASAREY
                     STIYNIPQYVINNRHGDCGMMVLTFITLCRLNGIPARWQSGWEFKPPQDSMHDWGEIY
                     LAPYGWVPMDVTYGRRATADESFRWFYLSGMDSYRLIFNDGMAADFYPLKIYPRSETI
                     DSQRGEVEWEGGNLYFNQWNWEHDWEIISEP"
BASE COUNT         6876 a         5496 c         4574 g         6940 t
ORIGIN      
        1 actattaatc cttacccaag ccgaacaatg aatatactca tagtccccaa tataaatggc
       61 aacgtgatta atttgctccg gatttggacc gaaaaacaac aaatcgccgg gcattaaatt
      121 ctccagtaaa ttggtcgtgt caaccgcaaa tccctgattg acctgcatgt tggcatccct
      181 ggctaataga taaccgttgc gacgataaac cgtttgggta aaaccactgc aatcaaaagc
      241 tttgggcgaa ttggcacccc agagataagg cctcccaagt aaattcttgg cagtcgttat
      301 aatcccctcc gcggtcggag ttcttgccag atattccgat tcgctaacca actcctggga
      361 cggaataaaa ccccggcgac cgtcaggaat ttcgacgcga gtccattgcc cttgcttgtc
      421 aatcatttta aatctattcc cgaggacaac atcggctacc ggcatagatt ttcggtcagc
      481 cttagagtaa atgatgccct cgaaatcatc gtaaacgact ttcgatgcat tttcccaggc
      541 ggtctttagt gagtcgccgc caactactaa tcggtcatcc tccatccatc ccaggtaacc
      601 atcatcggtc ttcaccaaaa aacagccttc ggcacttttc aatatttcga ccgtcaatcc
      661 ccgtaaggtt tgcgttacca tatcgttaaa taccgatggt ccactccgca aaggctcaac
      721 cgaattaatc acaatgccaa aattctttcc gttcaccgtt tcatcgggca atattttaat
      781 aagattttga attttaatag ctggcaaagt ttgttttatt tgattagtta aagcccggac
      841 tgcttcggga tggtcggttt ccccaactag attataatca ttatcctgat aatcaatttc
      901 aattttccat agcgcgactc gttgatccgg acaatatttc ttgcgaattt ctgcaatgat
      961 cgtttcaatt tgttgcttga cttctgttgt atctgcctgg caaattttac caatactaaa
     1021 aatgaagagc attaaggcaa acagttgaat ggtagttcgc aaaaaaacct ttacctggct
     1081 aaaccaagtg tcgttttata ttttccgtca tccctgaccg ctaaaattgc ctcggaaacg
     1141 accttgtcat actttaaatc ggctttaagt tgtccggcga ttccatccct gagcatcgcg
     1201 aactgattct caattccacg ctggatcatc aggtgattat tttcgaattc ggcttctttt
     1261 aatgaatcaa atatttcata taccggctgg aaatcaattt tgacgttctt taagcgttca
     1321 tcctggctaa ttatcttatc catctcacgg atgttctttt cggttttttc gataaattga
     1381 aatcccgaat gatcaatata atttttgaat tctttgatga tccgttcgtt aacctcttct
     1441 ggtaattcat tatgttcagt tttatactta acggcaaatg aataaaactg attttgacgc
     1501 cagagttcgg ttataaaatc gctctgctca tcgcttttaa taataatatc cggggtaatt
     1561 ccacccttgc tttttagctg acgcttatgg cgtgaataaa agatcgaatc ctgagacgga
     1621 ctggcggcga ttaactttgg atttttaata taatcaggct tttgaattaa tcgcccgctg
     1681 ggaagataat atttggcggt ggttatcttg agagaatgct ctttatcaac gcgaaaaact
     1741 gtctgtacta aacctttacc aaaactggga gtacccagaa cgattccgcg gtcgagatct
     1801 tgaattatac cggcaacaat ttcactagcg gaggcactgc cgccgtcaat taaaacggct
     1861 atttttacgg attcatccaa gagtggtcgg cgacgggcaa catcaactcg attggagctc
     1921 tcattttgac cacgcgtaaa taacaaagtg tctcccggct gagtaaacaa ctcggctacc
     1981 gttagtgctt ccgttaataa tccccccgga ttgccgcgta aatctaagat aagggcttca
     2041 agccggtcat gatctttaaa attttcaatt gcctgacgta cttccgccgt tgaccctttg
     2101 gaaaattcag ccaatcgaat ataaccgatg ccttcttcta aataatcggc ataataaaca
     2161 tcataaacct gaatttttgc ccgctctaaa atatatactt ttccttcatc agcccccgga
     2221 cgccgaatca ctaattcgac cttggtaccg acattgccgc gaatcaggcg ggtgcattta
     2281 tcgaggtcga gaccggttgt ccagattgta tcaattttta aaatttgatc accaggcaaa
     2341 atgttcgctt ttttagcggg actgccttcg atcggtgaaa ttacagtcag cgtgtcattg
     2401 cgcagactaa tccgtaagcc gactccgcca tattctcctt tcgataaaat ttccagcggc
     2461 tctttttcct cttcggtcat ataaactgta tagggatcga gctcacgggt catattccga
     2521 atattacttt cggttaaagc ggcggcatcg agtggatcgg cgtaattcag caatatttgc
     2581 tgaaagactg cattatacag cttgtaagaa tccgaaatcg ttttataaat atccttcgcc
     2641 gtaaaagcca ataagacact tactgaaacc agcacggcaa tccagatttt taaacctttt
     2701 ttaaatgata atttcatagt tgcatctttc taataatttt attgaattta tgccaaactt
     2761 catcagccac caactcttcc gttttagtgc catcaattaa acaaacacga ttgggttcag
     2821 cggcggcgat tttcaaaaat ccctgtcgga ttttttcctg aaaattatgt tcttcccttt
     2881 cgagacgatc aagatcacgc gtaccaaggc gctggagcct ttccgcaacc ggaatatcga
     2941 agataaaagt caaatcgggt accagattat cagcggcaat gcgattgagc atatttataa
     3001 aatttaggtc tattccccta ccatagccct gataagcagt cgtcgaatcg taaaaccgat
     3061 cgcagacaac gatttttccc gattccaaag ccgggcgaat caattcccga caaagctggt
     3121 gtcggctggc tgagtaaagt aaaagttcag taatggcaaa catttctgag ttggatttac
     3181 ttaaaagaat ttcgcggatt ttctcaccga tcggcgtacc gcccggttcc cgaaataacg
     3241 ccactggaat gttttctcgc tcacactttt ttatgaaata tttaatctgg gtcgtcttgc
     3301 ccgaaccgtc aatcccttcg aatgtgatta gcagaccttt agtcattaat gaatactttt
     3361 ctattgaatt ttactaatat actagaaact ctattttgac atttgcgcgg ctaataattt
     3421 taggaaaaat tggggcggac ggactgcttt tccggattga ttaacaaagg cgtgaatcgt
     3481 atagccgcta acgagaggct gagattcatt ttcacgtaga atttcgtaat caattcgaag
     3541 ccgggctgtt ggcattgcgg aaatgcgaga aataattgtt agcaaatcat caaaatgcgc
     3601 acttcgatga taaacgcaat gggcttccac taccggcatc gcataaccag cctgttcaaa
     3661 ttgactatac gggagtccaa tggctcgcat tatctcatcg cgggcggctt cgaagtattc
     3721 cagatagcgt gaataataaa caactcccaa ttgatcaaca ctcgagtaac gaactcgcaa
     3781 ctggtaatgg tgttctaaca tattaaaagc ctatcaaatt ttttatttgg atttggattt
     3841 aacgtcaacc actttccacg aacccatacg ggcatatttt tcaattcgct ggtcaactaa
     3901 ttcatctatc ggaatctgcg ttaattcagc caaatacctg agcaagacat ctttcagagt
     3961 ctgcgccatc tgatcgggat tacgatgggc tccgccaagt ggctccggaa taatttcatc
     4021 ggcgatttta aattcggcaa gatcacgggc ggttaccttc attgcctcgg cggctaacgg
     4081 cgccttggag ctatctcgat ataagatcga agcacaacct tccggagtaa tgaccgaata
     4141 ccaggtattt tccagtaaaa ggatgcgatc gcctaccccg atgccgagcg cgccaccgct
     4201 ggcaccttcg ccgattacta ccacaattat cggagtgcgc aagcgagcca tctcaaataa
     4261 gttacgggca atcgcttctg cctgtccacg ctcttctgca ccaactcccg gaaacgctcc
     4321 ttgcgtatca atgaaggaaa ttaccggacg tttaaatttt tcggcaagtt tcatagcccg
     4381 gcgggctttg cgataccctt ctggatgcgg cataccaaaa ttccggtaaa ggttttcctt
     4441 agtggttcgc cctttctgct gaccaatcaa aatgacttta taatcttcaa tttgcgccaa
     4501 tccggctaca acggctttat catcggcgta atatcgatcg ccatgaattt caatgaattc
     4561 ggaacaaatt cttttgacat aatccagggt atatggacgc tctggatgac gggcaagttg
     4621 aactctctgc cagcgagtta aattagagta ggtattttga atcaggcggt tgagtttgag
     4681 cttggctttt tcaatttccg gattgaggtc caccccgctg gccgcacttt gtgctcgcag
     4741 ttcctctatt tttttctcca actcgattat tggtttttca aaatcaagaa ctatttcagc
     4801 cattattttt tcaaattttt aaaaccgttt tcaaaataat ctcaacatcc agaaacaaag
     4861 aatggttttg catataaaaa tgttcgtaat tctgcttgtc gttttcgtcg gtgcgatgat
     4921 ttttacttac ccggaaaaga ctggtcagtc ccggtttaaa acgcaaattc cgttccccat
     4981 tttcggctgg taccaactca ctcccaacta aactgatatc gccttttaca acggaaaaca
     5041 gtaatgggaa acgatttagg aatggatgga catgattacc attccttttt accaaagtca
     5101 gcgcactgat ctgatgttgt tcagccccga taaaattggc tttctggaat ctataaccca
     5161 gcaaaaacgc tatcggaata acgaacggag atagcaaaat tatacttatt gaagaaatta
     5221 acaggtcaaa aatccgcttg gtaacccgat tgccgacatg aaacaagtta tagtcgatat
     5281 ttaccagtgg tatgtcttca atattttcaa cactcgattt ccctaaaatg taatccagat
     5341 tgcgcggaac gattttaata ttagttctgt actttttgat tttatctaca atccccagaa
     5401 tatcatcgtt attgaagcga tcggtagtaa agataatttc agtagcatga ttaatccgga
     5461 tgatttcagg taaatcgctt accaatccca gaaaaggaaa tccggcgatt tcctcattca
     5521 tataatcttt atcaacaaaa cccaaaattt caaaaccatg ttctatatga gtttttaact
     5581 tacgggcaat tcgttcacct tctttaccgg ctccgacgat aatagttcgc cgggacagca
     5641 gagcttttga caatgatgat gccggttgaa ttacacgacg tcgctgtaaa aataataata
     5701 acacccgcca gcctggtaaa aacagagtga caatgataaa actcaataac agcatattcg
     5761 gcgaataaat gacgtcctta aaaatcagat taaagatcag agaagcaacg aaactaagca
     5821 aggaagcaac aactgctcgt gaataggata attcccgacg atcatataag ccaatagaat
     5881 atcctattcc caaccaaacc agtaaataaa aaaccaacat tgatagtagc gaaggataat
     5941 cagggagagg cctgggttgt aattgatgag cgaccaaaat ggccgccacc aaagctatcc
     6001 cgtctacaat cattaccatt cggcgtcgga aaaatttcgc taccagtgat attatcccgc
     6061 ggaaaaaaat gccgactttg aaaacaatat cagtgattat cgaggaactt tttcccagat
     6121 gtttgctcgc aaaaatagcc attgctcggt aaaatgcgat aaaattatcg aaggcggcat
     6181 gtttggcact ttcgccttta taatgaataa ttttcgtatc cggcacataa taaattttcc
     6241 agccgctttt tttgattcga tagcagtagt ctaaatcctc accataaaga aaataggatt
     6301 catccaatcc gccaatctgt tcatatacgg ctctcctcat cattaaaaat gaaccgctaa
     6361 ccgcatcaac ttcggtgatt tgatcgggat ccagataggt aagattataa cgcgaaaaag
     6421 ccttcacttt tggaaataga cggctcaacc caagtaattt cggtaacgcc actgtcaaag
     6481 ttggaaaaga gcggcgacaa gccagttgga gtgaaccgtc agcgttcagt atcttacatc
     6541 cggcggctcc agcctctgga tgagccctaa aaaattcgat cattacttta attgtatctt
     6601 cctgaagcat cgtatccgga tttaataaca aaatatattc gccacgcgcg attgccaatc
     6661 cttgattgca tgccgccgca aatcctttat taaaatggtt ttctatcaat ataatttcgg
     6721 gaaatttatt ttttatgaca ttaacagtac cgtcaaccga atcattatca actacgatga
     6781 tttcacttga taaaccctca agggcattct tcaaagacaa aagacactgt tgaagaaatt
     6841 ctttaacgtt gtagctaaca atgatgaccg agatttgcat gtttacaaag atctgaaaat
     6901 ctgccgtaat ttcagtatcc agaccataaa tacagcttcc caaacaatat tttttgacat
     6961 tttagacaag ccttcggaac gctcgataaa aattatcgga atttccttga ttttaaaacc
     7021 cgcatcccaa acccggaagt gcatttcaat ttgaaaggaa tagccggagg attttacgcg
     7081 atctaaatta atcgcggcta aggcttcccg ccggtaacat ttaaaacccg cggttgcatc
     7141 cataatcggc attcgagtaa tcatacgagt atatttatta gccccataac tcaggagcaa
     7201 acgcttgata ggccaattaa tgacattcac tccattacaa tagcgcgatc cgattaccag
     7261 atcataaccg ttctcgcatt cttgaatgag tctgggcaca tcgtccggat tatgcgacaa
     7321 atcagcatcc atctccaaaa tatattcata atcacgttca agtgcccagc ggaaaccagt
     7381 gatataagcc gtccctaaac cgagtttagc gggtctattt atcataaaaa tatgaggatc
     7441 atcggcttgt aattccctta caatatcggc ggttccatca ggactattat cgtcaataat
     7501 tagaatatcc agctcgatcg gtaaagcctg taagcgttcg ataacggtgc gaatattgtc
     7561 tttttcgtta tatgtcggta tgattactaa tgacttcaaa ttgtttttct ttcatatttg
     7621 ttagagcttc tttaaccgaa tataaatgaa tcccaaattc agattgaatt ttttcacata
     7681 ctaaccccgc ccgaaaaggt cgaggggctt tctgattcag cgaacgggta gaaacagccc
     7741 gaatcaaagt cggatccagc tcaaaaaccg ccgcaatttg caaagcaaat tccagcctat
     7801 tgatataatc agccccgcca tagtgatata gcccattaat tccccgctca atcgccattt
     7861 caatcacttg cgccaagccg tcagcccagg taggattacc gaattgatca ttgaccacat
     7921 ttatcggctc ccggtgactg agtttattca ccacccaata aacaaagctg gcttcctgat
     7981 tagtcgagct accaaaaagg acatttgtcc ggaaaactgt tgctggtaac cctgattcta
     8041 aaacagcgcg ttcaccggct aatttggtct cgccataata attgatcggt tttaccggat
     8101 cattttctct atacggtcca ttgacgccat caaaaatata atcggttgaa atctgaataa
     8161 tcggagtatt agattttcgt aaagcggcta caagattcat tggaccgact gcattaactt
     8221 gccaggcggt ttcgcggtcg gtttcagcgc catctacatt tgtataagcc gcacaattaa
     8281 tgacccaatc cggttgaaat tcaccgatta tattttcgat ttcttccggc tttgtgatat
     8341 ccattttgcg gtagtcagtt tctaatttgt aaatcagttt atcatggagg tcagtcgcta
     8401 aaactctatg tttcgttgct agcaaacgag tgcatttttc acctaacatt ccgttagcgc
     8461 cggttattaa tattttcatt attagttgag tttgaagcct gccgccgcga attcgaccag
     8521 tttagctttt attttggcat tggagagcgc caaaattcga gccgcataga taatggcatt
     8581 aatgatccca ggtttgccga tcgccatcgt gcccaccgga ataccgcccg gcatttgcac
     8641 aatggaatag agggcatcta ccccgttcaa agacgaagca ttcaaaggga cgccaattac
     8701 cggcaaagta gtcagagccg ctataatacc cggcaaatgc gccgccatcc cagcacaggc
     8761 gataatcaaa cggccgtttt ctttttcaaa ctgctttaca aaatttacca attcgtcgtg
     8821 ctggcgatga gccgaaagga cttttatttc gcagtctaat tcaaaatatt ccagatactt
     8881 gcgcgcttgt tcgactgttt ctaaatcaga attgcttccg acaattaatg ctattttctc
     8941 cataaatcta cttgctccgc cgtaatttaa ttgaccaccc tataattgcc aaattccgac
     9001 taccgaccag ttctaactct atttccagga gcctttgtaa tcggtggtaa atgtcttagc
     9061 ggtaagttcg agcccgcgaa ataggactga caatttttca ccaggaccat acattagcat
     9121 gtcaataata aaggttcggt cggtgcgcca gtcataaaag gcatacgttt caaatggacc
     9181 accttgcgtt tcctcagtat ggtaccagag tccatacatt ttcaaagcat catattcacc
     9241 aaatttgaag aactgaaatc cgagaaagcg cttgtcagtc ataatcccgc catataattt
     9301 gccaatctca ttgcgttttt cgtatagtcc cacattcgtt aaccattccg tttgaatgcc
     9361 atctccccag gtaaccgaaa tccaacggta cggcaaacct cttccgagcc aaacgaaatt
     9421 attttcgggc aattctctga taatgatata atcgtgcgga atacgcatcg tccaacgata
     9481 tttctgccag agataattag ttagtttttt ttgctcatag cgtccatata tataatccat
     9541 ctggacttct tcgaatttct cttcaaactt tccataaagc cagccttttt gttccaagat
     9601 actgcgttca accatcttca aattgcggcc ggcaattaaa acgaacatct gatctcgagc
     9661 ccagttatcc ggaatggcaa acatatgaat gctatccttt tccgcggcta ttaaactttg
     9721 ctttggtaat ataccacggg caatttcagc cgccaggtct tcttgagtta aatcggctac
     9781 caaaatgatg tttttaaatt tgatatatga tggaaattct tctaccggca accattttgt
     9841 tgaaaaatag ggttgaaatt ccggcgtcct tattccgtag gaaaaagtcg tatcaattgc
     9901 cttacccagc acttctcgag tttgatcggt agcaaaaata taaatatcgt caacgccgcc
     9961 gatagattcc cgcagctggt tgcaattcat caacatcccg gaaataatca aaactaataa
    10021 tccaattttt ttattcattt ttatacccca tcaattattc aactttaaaa ttaaacattc
    10081 attggtatta agccaataaa tcttttaatc attcacccca ctccaaatct atccgtcgtt
    10141 ctctaactga aacatccgtc accattacca tcacttcatc gccaagccga aaaatgcgtt
    10201 gactatattt accacgtaga agatgatttt ccggatcgta aatataataa tcatcactca
    10261 gagtccgaat gtgtaccaat ccctcgacca gaaattggga aatttccaca aaaaatccga
    10321 attcacgcac gccactaatg acgcctttgt accattgacc gatcttgtcc gccataaaac
    10381 gcacctgctt gattttctgg taagtccgct cggcttccat cgcctcgatt tcacgttggc
    10441 tggcagaact ggcaatgtgc ggtaatgaac gccggtaaaa agccaggtcg ttagaatcat
    10501 aatcacctaa atagtttttc agcaaacgat gtaccaccag atcgggataa cgtcttatag
    10561 gcgcggtgaa atgggtataa tgaggaaacg ccaaaccgaa gtgacttaac ggttgatgag
    10621 aatagattgc tttagccatc gaacgcagtg taatttgttc aataaaattt ttatagggca
    10681 tttcagtcaa tccatttaga attttctgaa tatccatcgg tttgagcttg ggaggctggt
    10741 gcaaatcgag ccccaaattt ctcaaaatct gatacaattc agtgatattc tgttgcggcg
    10801 gttggggatg aattcgatag agaaaaggca gtttttcatt gcgccgttta atagatatat
    10861 attcagcgac tgcccgatta gccgccagca taaattcctc tattattcgg tgactgtcca
    10921 atctttcaga aggattgatt tcaatcggaa tcccagccgg tcccatctta aaaactggct
    10981 ccggaatgtc gaagtctata ctgcccgccg cccgtcggcg ctcaaaaaga gtgcgactaa
    11041 tctgacgcat attggtaaac atatcaaaat attcaccgct tttggtattc agaatctcct
    11101 gcacttcttc gtagctaaat cgcctaaacg agcgaattat actcggagca aatgaatacc
    11161 gcaccaccgc acctgctccg ttaagtgtca agataaccga aaatgccaat cgatcttctc
    11221 ccggtttcag actacacaac tcatcagaaa ttggcggcgg caacattgga ataacatttt
    11281 cgcccaggta aatactggta ccccgccgcc gagcctcgcg atcgagtaca ctgcccggcg
    11341 tcacataatg actaacatct gctatatgca cgcccagaag ccaattacca tctggacttt
    11401 tctccagcga tacagcatca tcatagtctt ttgcgtcagg aggatcgatc gtaaaaataa
    11461 acttatcacg caaatcgaga cgattgtctg cttttatttt ctgatttccg aggcgttcgg
    11521 cttcctgcca ggctttagtc ggaaatcggt cgctcagccg atacattttt tttacgatga
    11581 tcaaatcatc ctgcggattc tccggaaaac caagaatttg ttttaaagtg gctcgcggtc
    11641 gcaggcgagc gttgctccac tcgatgtcct ggatttcgac taattgacct tcccgcagac
    11701 ctttttccga acctactaat tcgatctcct caacagcggc cggatgttcc ggaatcacga
    11761 cccagtgctt atttttccgc ttcattgttc ctataattga tttttgacca cgattcaaaa
    11821 cttttaccac tttaccttcc ggtcgaattc cattgctata ccgcaatttt tcaattagca
    11881 cccggtcatg gtgtagcgca ttagcggtat gatcagcacc gacgaagatt tcttcgcccg
    11941 cgtcagtatt tacaaaagca aaaccgcgtc cggagaacga aattattcct tccacaaacc
    12001 gcgctttatc gggaattcga taccaaccgg accgcggatt ttcaatttct ccttctattg
    12061 ctaattcttt caatatttgt ttgaatctaa catacttatt ggaagagatg ccgacttctc
    12121 tagcaatttt tttctgttta taaatattgc ctttatttct ttggagaaat tgaataatcc
    12181 gctcgacaat ttgcgtatcc gttctaactt tcactgactt cggttttgaa ctgttattct
    12241 tgtgttttcc cattaaaatt tactctccaa aaatatatga attttggcgg ttgtaaatgt
    12301 atcaccttct ccgaatccgt aatcgagccc gataattccc atccgcgaat ccaaacgcaa
    12361 gccgacgcca taggaatgag gaaaatactt tttatctaag ccttcaaccg aacggcgata
    12421 atatccctga tcgaagaata tatgcgcccg accgttttta ccgataagcc agcgaatctc
    12481 attattgagc caggcaactt ccgagccacc aaacagctca gtagcatagc cacgcagact
    12541 gcgggaaccg ccaaaccagt agtagtcgga ataaaccggc acgccttccg atagccactt
    12601 tcctctgaga ttccagccac aggcggctac taattgggac cgaattttca catccaactc
    12661 accatttatt tcagtctgat attgccagag gcgaccgaca ttctgttcga tacgacgact
    12721 gagaaaggca ttaatcgcaa ccattatccc cttttgggga ttaagtggat aatcgcgacc
    12781 atcatagcgg aatgataatc ccaaaccgcg agtcgtgaga tcatttaatc cgagtaactg
    12841 gcgaccgcct gaggtcggca ttgtagatgt gtaattgaat ttcaaagccc cagagccgtt
    12901 ctggaataat ctgcggccga taccggcttc aaaagaacgt aaaaccacca gagtatctcg
    12961 caatatctga ctgaattggc cttccgcatt aaaattggtg tgccgaatcc acggctcgaa
    13021 atatctcaga tcaatttgtt gagaatagcg gttggatttc gcccaaaata cgctaagact
    13081 gcgaccgcgt ccgctaatgt tattcaaatt tagattcatc tcaccggtaa aatagccttt
    13141 cgtcgtagca gtggctggta tgtagccgac tattccactg aaggtattag tcgaatgttc
    13201 agtcagttga acaactaagc caaattgacc ttcgcgggtt tttactattt ccggttgaga
    13261 ttgcaccgtc agaaatgaat aactcttaaa attattcaga aaggctgaga ttttttgcga
    13321 atcgggtgat ttaccaagga ttgccgccga ttcgcgcttt aataattcag gagcggtgcg
    13381 tttgactccc tcatatatca ccgtatcaat gacaacccgc tcaccgcgat ccaatcgaaa
    13441 ttctgggttc agtttggtca gatttccttc agtttcaggc tgaaccgcac ttaaagtcaa
    13501 tactgggtaa aggtagccat cttttagcac ggcatctagt attttattcg tcaactcctc
    13561 atacgaatca gcggtaaccg gataattagt ataatccctg gcaatttcag taaaatcagt
    13621 caacttttcc tcaagcgccc taaccgtaat ttctccgaaa atcacttcag ctcgtgaagt
    13681 gtcctgagca attcccaaaa gcggtagaaa accgacaaat ccgattatca agaagatgac
    13741 aatctgtaaa ttttttcgcc tatgccgctg gtctgtcatt aaaacgaaag cctcacttcg
    13801 ccacttccta aatgatatac gcttcgctca ggctctttcc aaccttcgta attaactcga
    13861 atactaacat tcgaaccgac gcggtactca acagttgtac cccaaccacc ggtaagtcct
    13921 tccttcttgc cattgctcat ctcgtacgga attgctttcc ctttgggggt tacagatact
    13981 ttatccagtt caacaaatga tttccagcga cctttcccga caatttttcg ttcataactc
    14041 aattttacac cagctaggag cgactcaatt gggtcacttc ctttcgcttt gtcactgtta
    14101 agcgttattt ctgtaccgat tagatgaatc cgatccagaa aagttgacat tgtcaacttt
    14161 tcccgaacag tataaatatc acgtcctcta agcgaattta tctccgattg acgactataa
    14221 ttttttagcg ccgattcgga ctcgattgtg gtttttccta aataacgatt gcgataacgc
    14281 agagaccatt cgttggtgaa tgttttttct aatccccgaa catcagcttg agttatcaaa
    14341 tcggaggaat aaaaccgaag gcgcaaatcg ctttttcttt cccgcgaacg cagattaaca
    14401 tcttgttgaa ttatgcgtga ataatttgcc cagctggtat caacctgatt aatatccgtc
    14461 gcaaggtatt ttattattcc ctgcggatcg tggatttcct gccggagacg gacatcagtc
    14521 aaggtagaaa tacgttttag ccaatcaccg aaaatcggca ttttaacatt ggcaccgtca
    14581 aattgaaaac gcaatccatc ctcgatactg gtgatcggca ctttgatcga agaaggaaga
    14641 atccgtaaaa tgtagtcgcc ttgcggatcg ggaacgtaat cggcataagt cgaatcataa
    14701 atgtaatcac ccattccatc gccgacataa tagtagcgcc attctttttt gacagtctgc
    14761 tcgttttcaa ttttcagagt ggtttcccag cgataaggta atttgcgtgg ttcttgcttt
    14821 agggagaaat cgaggagatg aacaacaata tctgagttat tctgattata gaatttcttt
    14881 atacggtaga tataattcca ctgggatgag aatgaatgcc agtttatcaa ttggccgttc
    14941 agaccgatat tgcgagcgtt tgtccctttt atccaggtat ttgaatctga aaggtcatcc
    15001 cgacggagag tcgtctgaac ttgccaggtt agacgtcctg gactcgctga gccaatccca
    15061 tagatttgtt ccagaaagcg gaaattttca gttctaatag tgtcaccttc tcgtaattca
    15121 taatttagtg tagcaaacgg tttgactttc cataaatggg ttattaacct gaatcctcga
    15181 cgcgtccaat taatatcatt aaagccggtt tggttacgtt gaatgaattc ttcgctgatt
    15241 tggacatttt ccagcatttt gtagctcaaa ccggcgctgg ccgcaaatct attggaatta
    15301 aaatcaccac gcgaatactg accgccttcc gttttgacca ctaaattatt tttcaaagcg
    15361 tattgaattt gcccttcata atattgttca ccattggtcg aatcggaagg caagtcccat
    15421 ttccttcgat actcaactat ttgatgacga tcaatcggct cgaaacccca attttcctgt
    15481 ttataatgac cagcgagagc aatcttactg gattcggaaa gcggaatgat tagatttgac
    15541 gtaagatcaa ccgctaatcc ggcattatcc tcatcatcta gaggtgagaa taggttttga
    15601 tcgaaatctg aacgtgccac ttcagccttt atcgcaaagt tctgatgcgg acgccagtca
    15661 cttgccaaat gataaagcgt ccgactggtc ggcaatttta acggtttgac cggcaaataa
    15721 agtgctgagg caatctcgga agccgccgtc aagggattat ttttgtcaac ccattcaaaa
    15781 taagtcactt ctccgcggta aactttctta taactgccag ctgttcccac attataaaac
    15841 gtcgctgtat gagttccctg accgggaccg gcataaatca agattgaatc cactaaaata
    15901 taagagccgt ttgtatcgct cgtaatggta cttatgtaag cgtcttgagc gttgtcaccg
    15961 agcgatttca gcaatttttt atcggctccg ctaagcacta attcaatagg attttcgcgg
    16021 tcatcttttt cggtgatcgc ggcggcggaa acccttaatt tctcatcaaa taagttcgta
    16081 gtattgccag ccatgtaaat atttttctgg taaatcaaat cggaatattg aaaatcaaca
    16141 ctaatgcgcg attccgctgt aataatttga cgagtcataa aagttatctc ggcggtcgaa
    16201 taatcaatga tataatcatt gctttcgccg cggattaaaa ggcgaccgtc cagccagacc
    16261 ttctccgttc cagccagaac gattatatcg gtttcgccgt ttctgccgat cagctgatac
    16321 ggaccttgat tgctctcctc tccgagaaaa taattatgat aatattgacc tttggtaact
    16381 gctccactca taacattgcg catcggcggc cgctcgcttt ccagataaac gccctgcaat
    16441 ttgcgattgt aatttcccaa actgccggag ctataagcca tttcataatc accaaaagtc
    16501 agtcgttcat gcggcaagac aacggtaatg aacactttat caatttcatc aagggtttgc
    16561 gtattgcctt ctggttcaat aggaatattc tggtcggtaa ttgtgccgac gatcgcaata
    16621 tcttccgcaa ttttcccttg caactccagg ttcaagccgg actgaagcga cattccactc
    16681 tgactaccaa gggtcacacc gcggtaaagc gtaccggact tcaggaaatc ggtggtctcg
    16741 gtagaatact gagcgggcgg atgcgccgga taatcagttg aactgggttc ggcaatttca
    16801 ttacctgtat aaatgcgcgg cggcaggtta actattctat ttagcggcaa attgagcggc
    16861 aaatattgat atttggcaat aaaaaacgct gaatcagttc ttgtagaatg taaagtaata
    16921 gcaccggtca aaggatcgag actgattgaa tcagtaacgt tcaattcaat tgtttcaggt
    16981 atgacaaatc tatgagataa atagacaacc gaatcgctcg ccgcaatctt caaagtatcc
    17041 acctgccact gctgagcatt aagaagcctc gtgaatataa ataaaattcc taccagacga
    17101 gaggtaatcg tccatcgttt atatgctttt cgatataatg aactggcgtg attcaaaagt
    17161 tatattttaa ccaccagtta atcactaagg tttcgactga gaagtttcca tcgaaaaaac
    17221 taaaattgta caattactgg tcaacaaata aagccgatta ttggaaaaag tcgtggctat
    17281 aaaaggctcc agacgatcaa aatcagccaa agtcggctcg tcccagattt tcgatttcga
    17341 ataaaacatt cgcaaaaacg gagtctggtc aatcagaatc agccagtcgt tattgacggc
    17401 tgtcaatcca taagctttac atttgtttat tttcgaatta tggaatggaa ttaatccgag
    17461 cggtgtccca aaacgatcga attgcaccaa actgccatct tcctggagca cagttaaaat
    17521 acctttgcga tttatccgca gacttaccgg atggaccagc gcatagacac catattcgat
    17581 cccaccgaac caggtttgtt catttttttc gacataaagg cgcaaaatct cagccgtttc
    17641 accggataaa atgaagagtt cattaatctg cgaggctgtc aaagacatag gataaattaa
    17701 gcggcgagaa tcactacgta aatcctgagt cgcaatgtaa ttcaattgcc gatcataccg
    17761 caccaagcgg tgattattct ggtcggcaac aattacatct aatccggaaa aacacaaatc
    17821 gcgtgggcaa tcgaattgtc ccaatcccca tccaaaaccg ccgttgcggt tcagaatatt
    17881 gccattttgg tcaagacgat aaacttcact gaatttgctt tccagtagat aaatatcgtc
    17941 gaactcattg acgctgatgg cattaaattg cactccctct ctgactgatt gcggaacaat
    18001 ttctctctca aacctgaatt gcgctttgat cgtacaattc cagattaaaa taaaaattaa
    18061 aaggctgatt ttacttgatc ttttcatatt caatcggttt tcatagcgtt agttaaatat
    18121 acaatgtcgc ccgcaaggtt caaggctttt ttataaaagg atttgatgcg ttgaaaccaa
    18181 tttagcctct tcttaggagc tgcagaaatt ggtttggcta gtcacgccaa tacttagacc
    18241 gctacgcagt aagcacttgt ctaaaaatca tagattccta aatagcccgg agggcttcaa
    18301 gttttgtaga aattatctaa caagaaaatc tttctacctc ggagaggttt aacaaatttt
    18361 taaatcaatc ttaatcaaaa aaatcaaaca aatattggga atcaaatcta attccatatt
    18421 ttttcagaaa ttctatatat tcttcgcgaa aagtcctttt ggaatgatgc gcttcttgat
    18481 tttgaatata tctaaagacg tcatttattt gtgatctacc ataggaaaaa gcaccatatc
    18541 cttcctgcca attaaaatgc ccataacata acttattttt attaatgaaa attgaagtat
    18601 ttcgctttat atcatgcatt gtatcagaaa tggaaatatt aggattgagg ccaatgaaca
    18661 aatgagcatg gtcataatgt ccattgacta taattgattt atgccccaat ttggtgttaa
    18721 taccactcat gtattcaaaa actcgtttca gcatatctga attaaggacg gcatctctgt
    18781 tttttacagc aaaaactaac tgtaaataaa tttgggtgta tacccctgat ttcataaata
    18841 acctccttct taattttgct tcattcttga tattgatgac attgatttta acagtatctg
    18901 gtatatatca aaatagccaa tattctttat cctctccgag gaataacttt tctggctttt
    18961 ttattctaca aaacttcgac cgctacgcgg tataagaaaa aaggtaaaat atcttgtccc
    19021 gccgagcggg actccttctg agttaatctt atctaagaac atgcatctgc tcgattcgct
    19081 gaaaccaatt tagcctctcc ttaggagttg cagaaattgg tttggataat cacttactta
    19141 aaaacgctcc gaaaacggct tggattggtg acgttccact ccggattttt attcaaattc
    19201 tttttgataa aggtattgac gcgctcagcc cgatcaaaga ccgcgtcata gctccagttg
    19261 gattctggat tccagaaatc cggtgatgtg ttggcttcga tgcgcgtcag cacccgtccc
    19321 agcgcagacg gatcataacc ggaacgatag gcgtagatca ttccgagttg atcggcttcg
    19381 atctcatatt caatttggcg gcgttttgtt gccgtctcat agcaattcag ggcaattaga
    19441 tcgagttcat cggcggtagc gtcttcggtg acaatctctt ggtctaattc attaaatccc
    19501 tcgtcagcta caatcatcgt tttgcgtttg gcaatttcct cagcgccatg tttttgaaca
    19561 acgtgactaa tttcatgagc cagtatgacc gccagctcgg cttcatcctg taacagctcg
    19621 agcaaaccgg aagtgacaaa aatcataccg ttgggaatgg cataggctga cggatggctg
    19681 tcttttagaa tataaaattt aaagggataa tcgtacatat cggtattttc cagaacgata
    19741 ttgcctacca gattgatata tttcaattgc gattgattgg caaccaatcc gcttgctgca
    19801 attctggaag cggccgccaa tccaattttt tcttcttgca atgagactga gtgatcagtg
    19861 ttttctgatt tgattctctt atagcgtttg cggattttct ccgggtcgcg accgcgaaaa
    19921 gtctcttgtc taaaacgctg gaattcagcc ggagtaaaca attcaacatc atattgttcg
    19981 ataaaagccg gatcgccgtc gtgaaaattg acgaacttct gggcgaaacc tttgaccgct
    20041 ccactggcgg aagctctcga aatcatcatt gaaccatttc cactaatcga accggtcaaa
    20101 acgctaccgg ttggttcatc actcgcaata accgcatttt cggaaatcca gccgatttgt
    20161 cccgcacatt gaatattcat ccatcccgat tgagttccta caacagcaac ttggacgcct
    20221 ttctgcaaca tcccaatcag tggataatag gaaccggggc cttgtcggaa atgagtattc
    20281 tcgcgtttga ttaccacgga ttcttcacca aaaacggtaa caattaacac cgacactact
    20341 aaaattataa gaataaaacg tttcatattg cccctctcgc tttaatttat actttcgaaa
    20401 ttctatttca ttttgctcaa taatttaact tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20521 nnnnnnnnnn ncggctcttc acgacctttc acccgaatta aatcaagttc gcggcagata
    20581 aaattttctt ttaccaagga ataggtcgtc tcggaaataa tattttgcgt gccatagatt
    20641 ttgttgacgc cttccagacg cgccgcgaga ttaaccgtat cgccgatagc agtataatcc
    20701 atacggttcg ccgaaccgat attgccgacg acaaaattac cgctattaat ccccaatcgc
    20761 gtgattaatg ggatagtccg gttcttgact tcaatggtaa aagttgacag atttttaaaa
    20821 tccagcgccg cattacaagc catccgggcg tgttcgttag tcggaatcgg tgccccgaaa
    20881 attgccataa ttccgtcacc agtgtattta tcgagcattc cgtcgttctt gaaaattatt
    20941 tgttcgactt tggtaaaata ttggttcaaa aagccgacaa tttcaggtgg agtaaacagt
    21001 tccgcaattc cagtaaaatt ctgcaaatca gagaacatta ccgtagcgac gatttccttg
    21061 ccgcccatct caagacgctc gggattcctg gttaaaacct caactacggt gggatgcaaa
    21121 tagcgattga aaactttctt aatcattttc ttttcgcgcc cttcggtcaa ataattcact
    21181 aatagaacta ccgtaatact cagcacaaaa gcaatttcgg tggttacaac cggcataaac
    21241 attttcaagc gctggaaaat cattacactc attatcagcg gaataaataa aattatcaac
    21301 gaaatcccag ctgactgcca gattttcaac ttttgccaaa gaaacgccaa cagccagatt
    21361 agaattagag aaaatcccag ccagattata aatggtaaat tggaaatata atcgccccgg
    21421 ataatattcg agaaaatagt agcataaatc tcgactcccg gatatggctc gaccgacgaa
    21481 aatggtgtgg tcttcagatc gagtagtccc gccgcagtag cgccgataaa aaccgctttg
    21541 tcctggaaaa tcgccggatc aaggtctggc ttttgaccat tttgcacttg caaataggaa
    21601 ttcaacaact gggcaaaaga tacatattga aaagtatttc ctgctccgcc tggtccatac
    21661 caataaatat cgaaaaaacc actattatta agaggaacac gccgttttcc ggcgaagata
    21721 gtatgcctca tcgaatcata atcaatagag gaaatgtcat cgtaaatcat cacagccgcg
    21781 agcgccatat agggcacgat ttgatgatcc ttagcaaaga aaagcggaac ccggcgacag
    21841 actccatcat tatcggtgaa aaaattgacc gcgccgggat acgccatcgc tttttgaaat
    21901 cgctcaatcg gagccgtaac attcggatat ttgtaaacaa ttcgctgggg gacattgtaa
    21961 acgatttggc gattaaattg ggtcggcagt ttttcaacac taagatgcgt cgaatcttcc
    22021 atttgcattg ccagaatgac cttgccgctg gacagcattg cccgcgcaaa agctgaatca
    22081 gcatattcag cttgaatatt cagccggtca atatcaggcg tggaaaaaat aatatcatag
    22141 acaatgactt ttgcgccgca ggaattcaaa tagttagtcg ccatagcgta aatatcgcgg
    22201 ggccaaggcc aaagaatctg gaaatttttc tgaaaataat cgagactatt ttggtcaata
    22261 gcaataatta ccgcctccga cggatgttgt tcaacctgaa aatcgcgata caaaatatca
    22321 tttacttgat tttcccagcg ttgaatcaaa ccgttatgat aaagcaaacc gcttaataac
    22381 gttattatta gtgcaattcc cagcccaatt agtatttttt tagtattaat tttcaccgct
    22441 ggttttcatt agaaaattta cggttcagaa atgatctccc aatcatgttc ccaattccat
    22501 tgattgaagt ataaattgcc gccttcccat tcgacttctc cccgctgaga gtcaatggtt
    22561 tcactgcgcg gataaatctt cagtggataa aaatcagccg ccattccgtc attaaaaatc
    22621 aggcggtagc tgtccatccc gctcaaataa aaccatcgga aagattcatc ggccgtggcg
    22681 cgccgaccat aagttacatc catcggcacc caaccgtaag gcgcaaggta aatttcgccc
    22741 caatcgtgca tactatcctg aggaggcttg aattcccaac ccgactgcca gcgggccgga
    22801 attccattca ggcggcaaag ggtgataaaa gtcaacacca tcataccgca atcgccatgg
    22861 cgattattta taacgtattg aggaatatta taaatcgtcg agtattcgcg cgccgaagcc
    22921 caaactatat tgacgtcaat ccactcaaat aatttctgag caatacgata aggattggtc
    22981 tccgaaccga cgattctctg ggaaagttgc cggatttcat ccgtaaatac gatatgcggc
    23041 ggctcctcac tcaggaatgg cttcaggtcg ggattcggtt ctaccggaat tactttctcg
    23101 gcttttactg gagtatatga accataaatt tcgtaagtgt actcgaccga aaaatgggta
    23161 gtcgaatcct tgactgccgc tttttcgaaa taaatcgtcc gttgaagggt ttgatcggta
    23221 ttcgccagaa tataaatcgg cgggtcggtt tttagtaggc ggatattgac ctgccgatca
    23281 ggaatttcgc gcgggaaagg aatccagcaa cggatgattt ttccttccgg gacaacattc
    23341 ggatgaacag ttaggctttg ttttattttc attctaaccg gtaaaacggt agatttttgc
    23401 gtcgtttgga cggcttggac tattttgcga caatgtgaat ctagatcgaa cgccggttct
    23461 cttctagaat cggtaggtat ctgagaatca actttttgct taacggcttt taaagtgcga
    23521 tccaatcgga aaagattcgg cgcggcatcc ttgaaataca ttttttcgcc gtcaatcacg
    23581 aggtattcca acgacttttc cgattcccaa cgaaccaaat cagtgggcgt cacatcagga
    23641 acgtactgct tgatgtattc gataacttca gcctgactct tattaaaatc cagacgaatg
    23701 cgtttcattc tttccagttc aaattccagc gttcgtcttt cactaacgct gagagacgga
    23761 ttactaaata attcccggcg aattatttcc tcggcttttt taaactcgcc gttttcgatt
    23821 aaagcccgac agtcgcttcc ttcgcaatct atccaaatta ttaagcaaag caagccgatt
    23881 attcggtgga tatttttgat cattctttca attctctaca atttttaaaa ccagctgaaa
    23941 attagaacat agaaacttct ctgtccagaa atatttttac ctatca
//
LOCUS       DAOOMU010000026        23783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2017_length_23783_cov_18.505858, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000026 DAOOMU010000000
VERSION     DAOOMU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 23783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23783
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2017_length_23783_cov_18.505858"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..2717
                     /locus_tag="PLI55_03855"
     CDS             303..2717
                     /locus_tag="PLI55_03855"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPB00004.1"
                     /translation="MSESITIDFKNPNNESPEKNIPENSLSINPENIQVTPEKKPLFS
                     KNAMEVLTKRYLQHDKNGNAVETVEELFRRVAEAVAEAEANYSPRSSIPRTTEKFYQM
                     MIRKDFLPNSPTLMNAGRELGQLSACFVLPIDDSMESIFQTLKDTALIHKSGGGTGFS
                     FSRIRPKKSIVKTTSGVASGPVSFMKVYNAATEAVKQGGTRRGANMGILRVDHPDILE
                     FVDCKKNSNELTNFNISVAVTDAFMEKVRRREQYELVNPVDNTIVGTLNAAEVFKLIA
                     QNAWYNGDPGIIFIDRINQMNPTPKLGDIEATNPCGEQPLLPNESCNLGSINLANFIS
                     ENKIDYKRLGETIDLAVQFLDDVIDINKFPLKKIETMVKKTRKIGLGVMGFADVLFSL
                     KIPYDSDEALQLADELMKFIYNQTKEASTKLGESRGTFPAWNDSIYFPDGPKYRNSTC
                     ITIAPTGTISMIADCSSGIEPIFSLAFTKKVMDGTELLYVNPYFEQACKEAGIYSAEL
                     MHRVIAEGSIQHIAEIPEKIRRVFVTAHDISPEWHVRMQAAFQKWVDAAVSKTCNFPK
                     SATVQDVLDAYWLAYEAGCKGITIYRDGSRDEQVLYVGKQTDQAKSEQAVQPPIIDQT
                     LHPRIRPPETHGVTKRVRTGNGTLYITINEDDYGLCEVFTNIGKAGSQAQVESEAISR
                     LISLALRSGIDARDVVKQLKGIGGPSPIWENGTQILSTPDAIAKALEWYLDEREGKKK
                     TTGNQPSFSLLDVESTTTVKKPEPTSQHTAPHNKKSITTCPECGSTVYHESGCVTCPN
                     CGFSKC"
     gene            complement(3000..4079)
                     /locus_tag="PLI55_03860"
     CDS             complement(3000..4079)
                     /locus_tag="PLI55_03860"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HPB00005.1"
                     /translation="MALLEVCLVNDLPIIGYLSAILKKPDAIVWLRTPNQKKVVSLIE
                     TALSGQISSTFESREINPNDVPEFIEQCQKVLRDYPEHDVILNISGGSRLHAVLAAEV
                     FKNAGKEVILIDPDNSRIVDVKTGEVKTFQFNLTVNEYIALHGIKMESGTRFDPEIGK
                     RSALSFFIGNNLDRVMPFIDSHRDEWNNMGENKKTVHWKIDDQRLRFVVNYDAEQNVM
                     RFRFGTGENQKTVDIAGDGGNYLFNGGWLRELVFLRVHRQGDDVRLNVRIDRESVPEA
                     MRCESMIDICMMKGCNFYIFQCFSYPITRESFIELQAIHETVRILKAKGFIFMSHRPH
                     RGFIERASDIGLEVITGHRIANFSL"
     gene            complement(4244..5362)
                     /locus_tag="PLI55_03865"
     CDS             complement(4244..5362)
                     /locus_tag="PLI55_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPB00006.1"
                     /translation="MEKWRKLVQKSYTTVDQITKALNLPNDELAGLDKVFRIKVNPYY
                     FSLIKKKGDPIYRQVIPDKAELEDCGLEYDPLSEEKDSPVPSIVHRYPDRCLFLVSPE
                     CASYCRFCTRKRKVGDAQKISPRFIQQGIKYIAEHPEIRDVIISGGDPLMLSDQKIER
                     ILSSLRQIPHIQILRIGTRVPCFLPQRITTKLVRILKKYHPLYINVHFNHPDELTPEA
                     VKALSRLADAGIPLGNQTVLLKGVNDDPEVMKLLMQKLLQARVRPYYIYQADMVYGTE
                     HFRTRVEKGLEIIKALRGWTSGLAVPHFVIDAPGGGGKIPLLPEYVKSISDEEVVMCN
                     YEGKEFHYRQAGKPPAPPTCVRDIMAEALVAENDLALN"
     gene            complement(5366..5854)
                     /locus_tag="PLI55_03870"
     CDS             complement(5366..5854)
                     /locus_tag="PLI55_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB00007.1"
                     /translation="MNMIREIKSTDRAEILKILSDTQMFTTAEIRVAMELIDTALTKP
                     NQTDYLIFVAINGQKLAGYVCYGPTPATEGTFDLYWIAVAPQVQGKGIGTDLLTYVET
                     AVQAQHGNLIIIETSSQEKYLPTRNFYLKNGYAIAAQIKDFYRRGDDRVIFVKYFKQE
                     NK"
     gene            complement(5851..6867)
                     /locus_tag="PLI55_03875"
     CDS             complement(5851..6867)
                     /locus_tag="PLI55_03875"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00008.1"
                     /translation="MSSESQKIKILVVFNDLQSVDSNFDPDIISEIAVRDEAAAVYTA
                     LNNCGYVAEMLPILNIDEDLIKINSFQPDVIFNLCEGWQSRTDQEMNVAGLWEMLDLP
                     YTGNSPLTLGLAQNKVLSKNIFKAIGIPTPDFYVYESIPETCSLDFPLIAKPSREDGS
                     IGITQNSVVNDLIELKSIVGELLAKYKQPILVEKYIAGREFNVSILGNENPLVLPPSE
                     INFEQVSEEYFPITSYEAKWLSDHPVYQMTPPVCPAPISAELNEKLTSLSLAVYHALR
                     GRDYGRIDIRMDAAGNLFVLEYNPNPDISPSAGFVRALNAAGISYETFVVFLIKQALN
                     RRRR"
     gene            complement(6867..7919)
                     /locus_tag="PLI55_03880"
     CDS             complement(6867..7919)
                     /locus_tag="PLI55_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPB00009.1"
                     /translation="MKIALTYSTKEGLRKEYRKRFAVDPDDEDIPPDLFAEGDDPQTI
                     EAIVDALQLNNHQVVSFEGDDEVAHGLEQSRPDIVFNVTEGLFGDWRESYVPLICERL
                     GLPYTGSDPLTLAICLNKARCKEILSYYHIPNATFQVIYPGDDGFAENFKFPAIVKPL
                     AEGSSKGIFDNSVVYSKEQADDVIASCFAKYNNEPVIMEKYLAGPEFTVAILGNSPEM
                     EVLPIIGMDFSQLPTGAQPIYSYEAKWIWDRPEKPLNIFTCPAQIDDNLKNQIELLVR
                     RTCRILNIRDWCRLDVRLDENGVAHILEINPLPGILPNPEDNSCFPKAARTAGYTYPA
                     MLNQVVQVAARRYGLS"
     gene            complement(8069..9205)
                     /locus_tag="PLI55_03885"
     CDS             complement(8069..9205)
                     /locus_tag="PLI55_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HPB00010.1"
                     /translation="MTALLIYSLTVTARAQTNLGLWVIRDQLNSKEKIDAFVNFAVLN
                     DIKDLFVQVRGRGDAFYHSEIVVYSELLSNNNWDPLEYTLSQAHSYGLRVHAWVNVFL
                     LWSSDQKPKNENHLLYQHPEWCTVDANGIKDIQRRATDFNTLGTEGIYLSPLLPEVRK
                     YLIDIIQELATNYDLDGIHLDYVRYAKNCYDYNPIGRILFREKYGIDPLLLTLSDKSL
                     YKGLELAEIDSLMLKWEDFRRDAISDFVHNARKNLREIRPSLQLSAAVKPDPDDACKN
                     FFQDWQYWLRQDWLDFVVLMNYTKDTKQFQKILRKIDPNINKNNVWVGISVYNQSQYE
                     AMTKTILTLNNQFQNIVFFSYETFLKDPQYYPVVRKAFEIAHPR"
     gene            complement(9265..9858)
                     /gene="rplQ"
                     /locus_tag="PLI55_03890"
     CDS             complement(9265..9858)
                     /gene="rplQ"
                     /locus_tag="PLI55_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPB00011.1"
                     /translation="MRHQKKVAKLGRPTDQRKALIQSLTKAMLINGRIKTTDAKAKEL
                     RRVVERLITYGKRDTVHSRRLAFRFIQDRDLVNKLFEDIAPQYANRTGGYTRIIKLGY
                     RDNDNAAVSLIEFVDYKQPEEKKKKAGSEATKSVQGKKEEKSTEVEKEQTPAKTKPTK
                     KTQAKKEEKQSAQAEVKEDIPPENISGEKAEPASETE"
     gene            complement(9880..10860)
                     /locus_tag="PLI55_03895"
     CDS             complement(9880..10860)
                     /locus_tag="PLI55_03895"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPB00012.1"
                     /translation="MTVYDQIIARVVKDENSNDTHGIYYLQPLERGFGITIGNALRRV
                     LLTSIPGAAICSIKIDGVLHEFSTIPGVREDVAEIILNLKQVRLKLFDPKPDKVTLHF
                     KGAGEFTAGMIGENITQFKILNPDLHICTMNEDADFNIEIRIARGTGWVPAEKNKLPD
                     YPIGTIFIDSIFSPILNVSYKVENVPGTAREILEKLTLEITTDDSITPDESLDLASKI
                     LRDFFSLFSRVEAKPIEFQREEPSEDVLKIRNLLKKSVDEMELSVRSYNCLKANNIRT
                     IADLVSHEEADMLKFKNFGRKSLNELMTKLKSMGLEFGMDVDKYLVEKEE"
     gene            complement(10872..11504)
                     /gene="rpsD"
                     /locus_tag="PLI55_03900"
     CDS             complement(10872..11504)
                     /gene="rpsD"
                     /locus_tag="PLI55_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPB00013.1"
                     /translation="MARYTGPVCKLCRREGKKLFLKGERCNSTKCSFETKSYIPGQHG
                     SQTAHPRLSNYGIQLREKQKIKRIYGVLEKQFRQYFELANRQKGVTGTNLMKILERRL
                     DNVVYRLGFASSRRMARELVSHRHFTVNNRVVDVPSYQLKAGDVIQVREKSKKLQVIH
                     DSMKRIRGEHELPWLILDKAKMQGIFIQVPERDQMNVDVNEQLVVELYSK"
     gene            complement(11527..11919)
                     /gene="rpsK"
                     /locus_tag="PLI55_03905"
     CDS             complement(11527..11919)
                     /gene="rpsK"
                     /locus_tag="PLI55_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPB00014.1"
                     /translation="MPPKRSTVKKKKRTKVESEGIATIKATFNNTIISLTDNYGNVIS
                     WASAGTAGFKGSRKNTPYASQQAATKAAQEAINLGLQRVEVRVKGPGAGREAAIRALN
                     LAGLQISAIKDVTPIPHNGCRPPKRRRV"
     gene            complement(11937..12323)
                     /gene="rpsM"
                     /locus_tag="PLI55_03910"
     CDS             complement(11937..12323)
                     /gene="rpsM"
                     /locus_tag="PLI55_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPB00015.1"
                     /translation="MARIAGVDLPNEKKVKIALAYIYGIGRANAMDILQKAGVDPEIR
                     VKALTEEQVRDIRNVISNEYKVEGTLRTEVQMNIKRLMDIGCYRGLRHRRGLPVRGQR
                     TKTNARTRKGPRRTVSRKKVKVGKKG"
     gene            complement(12343..12456)
                     /gene="rpmJ"
                     /locus_tag="PLI55_03915"
     CDS             complement(12343..12456)
                     /gene="rpmJ"
                     /locus_tag="PLI55_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002344960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPB00016.1"
                     /translation="MKVRSSVKKICNKCKIIRRKGVVRVICTNPKHKQRQG"
     gene            complement(12473..12691)
                     /gene="infA"
                     /locus_tag="PLI55_03920"
     CDS             complement(12473..12691)
                     /gene="infA"
                     /locus_tag="PLI55_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPB00017.1"
                     /translation="MVKEKLIKVDGIIKENLPNASFRVVLENGHEVLAHVSGKMRMHF
                     IKILPGDKVTMELSPYDLTKGRITYRYK"
     gene            complement(12704..13453)
                     /gene="map"
                     /locus_tag="PLI55_03925"
     CDS             complement(12704..13453)
                     /gene="map"
                     /locus_tag="PLI55_03925"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007553999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPB00018.1"
                     /translation="MVHIRTAEEIEKIRESCRIVSETLDLLSREIKSGITTLYLDHLA
                     AEFIKSQGGHSAFKGYNGFPGNICTSIDEQVVHGIPSNRVLKEGEIISIDVGVKKNGY
                     YGDAAMTFAVGTVSDDRLQLMRVTEDSLYQGIAQARPGNHLSDIGHAIQTHVESYGYG
                     VVRELVGHGVGRELHEPPEIPNYGEPGRGPVLKPGMCLAIEPMITMGSYEVRLLKDGW
                     TVITEDHLPSAHYEHTIAIGPDGPQILSQRK"
     gene            complement(13463..14803)
                     /gene="secY"
                     /locus_tag="PLI55_03930"
     CDS             complement(13463..14803)
                     /gene="secY"
                     /locus_tag="PLI55_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPB00019.1"
                     /translation="MIQQFQSVFKIPELRKRIGYTLALLIVFRIGAHIPIPGIDTEVL
                     GAAMSSFANTLFGLYDMFSGGAFAQATLFALGIMPYITASIILQLLGAVIPYFQKLQK
                     EGEEGRKKLTQMSRYGTVIIAALQSFGVAQFLMSPQMSARVGNQMLPVVPRPGVGFVL
                     MTMLTITTGTVLLMWLGERMTERGIGNGTSMIIMVGILARLPNVFVSEITLIREGVRG
                     LFTEIILIVLMIVIVGFVVLLTQGTRRIPVQYAKRVVGRKVYGGQATHIPLRVNTAGV
                     MPIIFAQSIVFIPSTIATFFPNSNFVQGMMRIFSVQSIVYWLIYGVLIVFFTYFYTAI
                     AFNPVEVADNLKKYGGFIPGIRPGKKTAEFIDNILTKVTLPGSIFLAFIAVFPYILMK
                     IMDINYDLASFFGGTSLLIVVGVALDTLQQIESHLLMRHYDGFLKSGKLKGRRG"
     gene            complement(14800..15237)
                     /gene="rplO"
                     /locus_tag="PLI55_03935"
     CDS             complement(14800..15237)
                     /gene="rplO"
                     /locus_tag="PLI55_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPB00020.1"
                     /translation="MGLNTLKPAYGSVKNKKRLGRGPGSGHGGTAGRGNKGTGARGSN
                     KKPVYVEGGQMPIYRRLPKRGFKNLFKEEFQVVNLDHIARLQITEVNPEILQQKGLIK
                     SLKKPVKILGRGELSGAITVVAHAFSTRAKEKITASGGKVELL"
     gene            complement(15241..15444)
                     /gene="rpmD"
                     /locus_tag="PLI55_03940"
     CDS             complement(15241..15444)
                     /gene="rpmD"
                     /locus_tag="PLI55_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPB00021.1"
                     /translation="MTKKDKTAMLKITQVKSGIGYCEKTKRTLIALGITKMNQTVLLP
                     DNPAIRGMINSIPHLLKVEEIKE"
     gene            complement(15441..15950)
                     /gene="rpsE"
                     /locus_tag="PLI55_03945"
     CDS             complement(15441..15950)
                     /gene="rpsE"
                     /locus_tag="PLI55_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPB00022.1"
                     /translation="MRKSIDPTQLELLEEKVIKINRVAKVVTGGRRFRFNALVAIGDG
                     KGHVGIGLGKALEVSSAVAKGKENAKKNLVKIPVINGTIPHEVQAKYGAAKVILRPAT
                     PGTGVIAGGAIRAIMESLGVRDVLSKSLGSNNSHNVVKATMLALSKLKDPYMVAHRRE
                     KTLQEIFGS"
     gene            complement(15969..16343)
                     /gene="rplR"
                     /locus_tag="PLI55_03950"
     CDS             complement(15969..16343)
                     /gene="rplR"
                     /locus_tag="PLI55_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPB00023.1"
                     /translation="MNKVKNIKAFARQRRQKRNRHKIQGTSERPRLTVFRSLNHIYAQ
                     VVDDNLHKSLLAVSDLTPEVREQIKSNTKKSDRSALVGKTLAEKAVAKGITKVVFDRS
                     GYQYHGRVKALAEAARQAGLEF"
     gene            complement(16355..16897)
                     /gene="rplF"
                     /locus_tag="PLI55_03955"
     CDS             complement(16355..16897)
                     /gene="rplF"
                     /locus_tag="PLI55_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPB00024.1"
                     /translation="MSRIGKKPIEIPKEVNVTIQDCSITVKGPKGELKWDFPSDMKVQ
                     INDSQIIVTRPSDSRNHKALHGLTRVMIANMIQGVLQGFSKTLEIEGVGYSAEMKGKS
                     VQINVGYSHAILVTPPEGVSFEVPKALTIVVKGIDKQKVGQIAAKIRSIKPCEPYKGK
                     GIRYQNEHIRRKAGKKVGVK"
     gene            complement(16921..17319)
                     /gene="rpsH"
                     /locus_tag="PLI55_03960"
     CDS             complement(16921..17319)
                     /gene="rpsH"
                     /locus_tag="PLI55_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPB00025.1"
                     /translation="MAVTDPIADYLTRIRNAQAANHRWVDIPASNLKKRISLILKHEN
                     FIKDFILIKDGKQGLIRIYLRYSKTGEPMIEGLQRISKPGRRVYAASDNIPRVVGGMG
                     IAILSTPMGVLTDKVARKNNVGGEVLCYVW"
     gene            complement(17336..17521)
                     /locus_tag="PLI55_03965"
     CDS             complement(17336..17521)
                     /locus_tag="PLI55_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013740294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPB00026.1"
                     /translation="MAKKSLIAKARRNPKFSTRRVNRCSICGRPRGYYRKFGLCRLCF
                     RELALRGEIPGITKASW"
     gene            complement(17523..18107)
                     /gene="rplE"
                     /locus_tag="PLI55_03970"
     CDS             complement(17523..18107)
                     /gene="rplE"
                     /locus_tag="PLI55_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPB00027.1"
                     /translation="MTAYETEIVPAMVKKFGYKNIMQVPKLVKISINMGVGKTKEDPA
                     SLKKAVEDIRIITGQQPMVTKAKRAISNFKIKTGDPVGCAVTLRRFRMYEFMDRLIST
                     ALPRVRDFSGLSDKSFDGRGNYSIGLKEQIIFTEIDYDKVDHIRGMNITFTTTAKTDT
                     EAYELLSLLGLPFKKRAIKESTTGGEVKTAMEIN"
     gene            complement(18154..18468)
                     /gene="rplX"
                     /locus_tag="PLI55_03975"
     CDS             complement(18154..18468)
                     /gene="rplX"
                     /locus_tag="PLI55_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPB00028.1"
                     /translation="MLHIRKNDTVMVITGESKGKIGKVLKVFPDKERVIVEGVNFIKR
                     HTRPNQQNPQGGIIEKEAPIHISNVKLVHADQATKVGYRTLKDGKKVRVARETGEIID
                     AI"
     gene            complement(18481..18849)
                     /gene="rplN"
                     /locus_tag="PLI55_03980"
     CDS             complement(18481..18849)
                     /gene="rplN"
                     /locus_tag="PLI55_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPB00029.1"
                     /translation="MIQQESRLKVADNTGAKEVMVIRVMGGSHRKYGTVGDTIVVTIK
                     QATPGGMVKKGEKSKAVVVRTTKEVRRKDGSYIRFDDNAAVLIDNNLEPRGTRIFGPV
                     ARELREKNYMKIVSMAPEVL"
     gene            complement(18873..19124)
                     /gene="rpsQ"
                     /locus_tag="PLI55_03985"
     CDS             complement(18873..19124)
                     /gene="rpsQ"
                     /locus_tag="PLI55_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPB00030.1"
                     /translation="MISSDRRKILIGTVISHKQDKTAVVSVERTVKHSTYGKYIRRTS
                     KFYVHDAENKCHEGDKVKIVESRPLSRLKRWRLLEIIER"
     gene            complement(19143..19361)
                     /gene="rpmC"
                     /locus_tag="PLI55_03990"
     CDS             complement(19143..19361)
                     /gene="rpmC"
                     /locus_tag="PLI55_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPB00031.1"
                     /translation="MKKSTLDELTVNELETKLRDNLTALENLRFQKALQQLENPLKIR
                     HVKREIAQIRTVLHEYELGIRSKDKPNK"
     gene            complement(19363..19782)
                     /gene="rplP"
                     /locus_tag="PLI55_03995"
     CDS             complement(19363..19782)
                     /gene="rplP"
                     /locus_tag="PLI55_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPB00032.1"
                     /translation="MLAPKKIKWRKQQRGRMTGIATRGNAVSFGHYGLKAMEPGWIDS
                     RQIEAVRVAISRQIRKHGKMWIRIFPDKPITKKPAETRMGKGKGTPEFWVAVVKPGRI
                     LFEIEVASEQEAKDIFKTATHKLPIKTKVVARRELGA"
     assembly_gap    19872..19971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19972..20291)
                     /gene="rpsC"
                     /locus_tag="PLI55_04000"
     CDS             complement(<19972..20291)
                     /gene="rpsC"
                     /locus_tag="PLI55_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPB00033.1"
                     /translation="MGQKTHPIGFRLGFNKEWSSYWFDERNFTEKLTEDIKLRRYVLK
                     RLPNAGISRVDIYRTPKKITITINTSRPGIVIGKKGEEVELLRLELQKVTKTDVQINV
                     NEIKR"
     gene            complement(20303..20671)
                     /gene="rplV"
                     /locus_tag="PLI55_04005"
     CDS             complement(20303..20671)
                     /gene="rplV"
                     /locus_tag="PLI55_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPB00034.1"
                     /translation="MEAKAVHRYVRQSARKVRLVADLVRGKDVETALNLLHFNSKKAS
                     ETIEKVVRSAVSNLLNSEGGSKLNPEDLYIKTIYVDGGSIAKRFRAGSLGRASIIRRR
                     LCHITVVVAEKENKQKSKVQ"
     gene            complement(20680..20955)
                     /gene="rpsS"
                     /locus_tag="PLI55_04010"
     CDS             complement(20680..20955)
                     /gene="rpsS"
                     /locus_tag="PLI55_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPB00035.1"
                     /translation="MSRSLKKGPYIDVKLMKKIEQMNSSGKKRVIKTWARRTTISPEF
                     VGHTFAVHNGNKFIPVFVTENMVGHKLGEFAHTRIFRGHSGAEKKGA"
     gene            complement(20972..21799)
                     /gene="rplB"
                     /locus_tag="PLI55_04015"
     CDS             complement(20972..21799)
                     /gene="rplB"
                     /locus_tag="PLI55_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPB00036.1"
                     /translation="MGIKTIKPITPGQRFKTVDTFEDITKKRPEKSLLEPITQSAGRN
                     NYGRVTSRHRGGGQKRAYRNIDFKRNKYGIEATVKAIEYDPNRSARIALLFYKDGEKR
                     YIIAPDGLVTGSVVLSGEEAPIKVGNCLPLKNIPAGTFVHNIEMRPKKGGQIARGAGT
                     AAQIMAKEGKYVTLKMPSGEIRLINEECYATIGVVSNKDHENINIGKAGRSRWMGRRP
                     HVRGVAMNPIDHPMGGGEGKSSGGRHPCSPWGQLSKGYRTRKKRNPSNKFIIKRRTK"
     gene            complement(21804..22124)
                     /gene="rplW"
                     /locus_tag="PLI55_04020"
     CDS             complement(21804..22124)
                     /gene="rplW"
                     /locus_tag="PLI55_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009353943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPB00037.1"
                     /translation="MSSKSIIIKPILTEKINELQESQNKYGFKVHPSANKIEIKKAVE
                     DKFGVKVEKVATQNIMGKTKLMTVRSGGRVIRTTGRRSNWKKAIVTLKEGEKIDFYQG
                     ETAV"
     gene            complement(22127..22774)
                     /gene="rplD"
                     /locus_tag="PLI55_04025"
     CDS             complement(22127..22774)
                     /gene="rplD"
                     /locus_tag="PLI55_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPB00038.1"
                     /translation="MSIEFSVYKLDGSDSGQKLVIETPAFDIEPHDHAIYLAVKTEMT
                     NSRQGTHSTKTRGEVRGGGKKPWKQKGTGRARSGSRRSSVWVGGGRAFGPKPHPYKMK
                     INKKVKSLARQSALAYRYREGALKIIEELSFDEPKSRNIRELLKKFDLLDKKVTILTA
                     NLNTNLLLASRNFYNVLVLEAEKASTYDLLDCEVLLIDKSGFEKLNNALLGDSRN"
     gene            complement(22780..23400)
                     /gene="rplC"
                     /locus_tag="PLI55_04030"
     CDS             complement(22780..23400)
                     /gene="rplC"
                     /locus_tag="PLI55_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPB00039.1"
                     /translation="MMIGIIGKKIGMSQIFKENGELIPVTVIAAGPCKVVQVKTNEKD
                     GYASVQLGFMEAKERLMAKPNLGVFKKNNITPLKYLVEIRDYDPKFCQVGETVTVALF
                     NPGDKVKITGISKGKGFQGVVKRHNFGGGPKTHGQSDRLRAPGSIGASAYPSHVIKGM
                     RMAGRLGGKRCTVNNLTVVKIDPESNLLYIKGAVPGSKNSILTIRR"
     gene            complement(23409..23717)
                     /gene="rpsJ"
                     /locus_tag="PLI55_04035"
     CDS             complement(23409..23717)
                     /gene="rpsJ"
                     /locus_tag="PLI55_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPB00040.1"
                     /translation="MPGQRIRISLKAYDHNLLDKSTRKIVATAKSTGAMVSGPIPLPT
                     QRTVYTVLRSPHVDKKSREQFQTKIHKRLIDILNSTSKTVDALMKLDLPAGVDIEIKV
                     "
BASE COUNT         6270 a         5367 c         4773 g         7273 t
ORIGIN      
        1 tgaactacac ccgcaatgca aaaaagcgtt gataatttaa tagcactaaa taattttaaa
       61 aatgttttta attacttgat tggataaaca ttacccagtc tattttaaag tttttgtcaa
      121 ggtaattagc atttatcggc taatgcttat tatccaacaa tttgtgtttt tctcgtcgag
      181 aaatacaaaa tcttgtgttt ttccttgact acttggtatg aacttactaa ctttcaccca
      241 gtttaacttt tgaaaattaa aatgggctca atcaattaac tcaaaatagg agattttcgc
      301 gaatgtctga gtcgattacc atagatttta aaaatcccaa taacgaatcg cccgaaaaga
      361 atattccaga aaattctctg tcaattaatc ccgaaaatat tcaagttact cccgagaaga
      421 aacctttatt ttccaaaaat gcgatggagg tattaaccaa acgctacctt cagcacgata
      481 aaaatggcaa tgccgtcgaa acggtcgagg aattattccg cagagtggct gaagcggtag
      541 ccgaagccga ggctaattac tctccgcgct ccagcattcc ccgcacaacc gaaaaatttt
      601 accagatgat gattcgtaaa gattttctcc ctaattcacc aaccttgatg aatgctggcc
      661 gtgaacttgg gcagctttcg gcttgctttg tcctaccgat tgacgattcg atggaatcta
      721 tatttcagac tttaaaagat accgcactaa ttcataaaag tggcggtgga accggctttt
      781 ctttcagccg gattcgcccc aaaaaaagca ttgtaaaaac taccagtggg gttgccagcg
      841 gaccagtatc ctttatgaaa gtttacaacg ccgccacgga agccgtcaaa caaggtggca
      901 cccggcgggg cgctaatatg ggaattctgc gcgttgatca tcccgatatc ctcgaattcg
      961 tagattgcaa gaaaaatagc aatgagctaa cgaattttaa tatttcggtc gccgtaaccg
     1021 atgcctttat ggaaaaagtc cggcgacgcg aacagtatga actggtcaat ccggttgaca
     1081 atactatcgt cggaactctt aatgcggctg aagttttcaa actgattgcc cagaatgcct
     1141 ggtacaacgg cgatcccggt ataattttta ttgaccgtat taatcagatg aatcccactc
     1201 cgaaattagg cgatattgaa gccaccaatc cttgcggcga gcaaccgctc ctgcctaacg
     1261 aatcctgcaa tctcggttcg atcaacttag ccaattttat ttccgagaat aaaattgact
     1321 ataagcgcct tggcgagact attgatttag ccgttcaatt tctggacgat gtaattgaca
     1381 tcaataaatt ccctctcaag aaaatcgaaa caatggtaaa aaagacgcgc aagatcggtc
     1441 taggagttat gggatttgcc gacgttcttt tcagcttgaa aataccttac gattcggatg
     1501 aagcacttca gttagccgac gaattaatga aatttatcta caaccaaacc aaggaagcct
     1561 ctaccaaatt aggcgagagt cgtggtactt ttcctgcctg gaacgatagc atttacttcc
     1621 ccgatggtcc caaatatcgc aattcaacct gcatcacgat tgcaccaacc ggtacaattt
     1681 caatgattgc cgactgttcg agtggcattg aaccgatttt ttcattagca ttcactaaaa
     1741 aagttatgga tgggaccgaa ctgctttatg ttaatcctta tttcgaacag gcttgcaaag
     1801 aagccggtat atacagtgca gaattgatgc atcgggtcat cgccgaaggc tcaatccagc
     1861 atattgcaga aattcctgag aaaataaggc gggttttcgt tacggcacat gatatttcac
     1921 ccgaatggca cgttcggatg caagccgctt ttcagaaatg ggtagatgcg gcggtttcca
     1981 agacctgcaa cttccccaaa tcggctacgg tgcaggatgt cctggacgct tattggctgg
     2041 cttatgaagc cggttgtaaa ggcatcacaa tttatcgaga cggaagtcgg gacgaacagg
     2101 ttctgtacgt cggcaaacag accgatcagg ctaaatcgga gcaagccgtt cagcccccga
     2161 taattgacca aaccctgcat ccgcgaattc gtccacccga aacccatgga gtaaccaaac
     2221 gcgttcggac gggtaatggc actttgtata ttacgatcaa tgaagatgac tacgggctgt
     2281 gtgaagtctt tactaacatc ggcaaagccg gtagtcaggc gcaagtcgaa tccgaagcca
     2341 ttagtcgttt gatttcgctt gccctgcgtt ctggaattga cgcccgcgat gtggttaagc
     2401 agttaaaggg catcggtggg ccttcgccga tctgggaaaa cggcactcaa attctctcga
     2461 ctccggacgc tattgccaaa gccctcgaat ggtatctgga cgaacgcgaa ggcaagaaga
     2521 aaaccactgg caaccaaccg agtttttcgc tactcgacgt tgagagtaca acgacggtaa
     2581 agaagcccga acctacttca caacacaccg cgccccataa taaaaagtca attacgacct
     2641 gtccggaatg tggttctacc gtttaccatg aaagtggctg tgtgacctgc ccgaattgtg
     2701 gcttttcgaa atgctaaact gcgtctgggt ttagaatcta tactttgata catattttca
     2761 tatacctcca agctataccg aaagcctaag gtcaccgagc gattaaatat tttgcgtatc
     2821 tgccatgtga atttgaaaac tcgaaaattc tatctgcctt ggtcagtatt accgtttatc
     2881 gtattttatt tttcaatagc ttaatccgtc aacctagtaa tcttacagta tgtttctctc
     2941 cttgcccctc attaattcaa tgcaaggaat atcagtataa ttctttagct gataatggat
     3001 tacaaactaa aattagcgat ccgatggcca gtgataactt ccaagccaat atcgctggcg
     3061 cgttcaataa agccccgatg cgggcgatgg gacatgaaaa taaatccttt agctttcaat
     3121 atcctaacag tttcatgaat agcctgaagt tcaataaatg actcgcgggt gatgggatag
     3181 gagaaacatt gaaaaatata aaaattacac cctttcatca tacagatatc aatcattgat
     3241 tcacaacgca ttgcttcagg gacactctcc ctgtcaatcc gcacattcag acggacatca
     3301 tcgccctgac ggtgcaccct gagaaatacc aattcacgca accacccacc attgaaaagg
     3361 taattaccgc cgtcgccggc aatgtcaaca gttttctggt tttcgcccgt tccaaagcgg
     3421 aagcgcataa cattctgttc cgcatcataa ttaacaacga aacgcaatcg ctgatcatca
     3481 attttccaat gaaccgtctt tttattttcg cccatattat tccattcgtc tcgatgcgaa
     3541 tcaataaacg gcatgacccg atctagatta ttgccgataa aaaatgaaag ggcgctacgt
     3601 tttccgattt ccggatcgaa gcgcgtcccg gattccattt taattccgtg cagagcaata
     3661 tattcattta cagttagatt aaattgaaaa gtcttaacct cgccggtttt gacatccaca
     3721 attcgcgagt tgtcgggatc aatcaggatg acttccttac cggcattttt aaaaacttca
     3781 gccgccagaa cagcatgaag acgcgatccc ccactaatat tcagaataac atcatgttca
     3841 ggatagtctc tgagaacttt ctggcactgt tcgataaatt cgggaacatc attcggatta
     3901 atttcacggc tctcgaatgt cgagctaatt tgtccactta aagcagtttc gattagcgaa
     3961 acgacctttt tctgatttgg ggtgcggagc caaacaatag cgtccggctt ttttaaaatt
     4021 gcacttagat atccgattat cggtaaatcg tttaccaaac aaacttccaa cagcgccata
     4081 ttcatctccc atttctacta aaacgaatca tttattaaac atttttagaa tttaataatt
     4141 aatttgagtt tttcaataag tgattttaat aaaagaagat aggctggtaa agcgagtaat
     4201 tatccgaatt accttaccct gaaaaattaa aaggtgatgg aatttaattt agggctaaat
     4261 cattctccgc aaccagcgct tccgccataa tgtctcttac acaggtaggt ggtgcgggtg
     4321 gcttacctgc ttgtcggtaa tgaaattctt tgccctcgta attgcacata acgacttctt
     4381 cgtcagaaat ggacttgaca tattcgggta ggagagggat ttttccgcct ccaccgggag
     4441 cgtcaatcac gaaatgcgga accgccaaac cggaagtcca gccgcgcaga gctttgataa
     4501 tttctaaacc tttctcgact cgggtgcgga agtgttcggt accatagacc atatcagcct
     4561 gataaatata ataaggccgg acgcgcgctt ggagcaattt ctgcatcagt aatttcatca
     4621 cttcagggtc atcgtttacc cctttcaaga gtacggtctg gtttccgaga ggaataccag
     4681 catcagccag gcggctaaga gccttgaccg cttccggcgt caactcatcc ggatggttga
     4741 agtgaacgtt aatgtatagt ggatgatatt tcttcaggat ccgaactaat ttcgtagtaa
     4801 ttcgctgagg cagaaaacag ggtacgcggg tgccaatgcg tagaatttga atatgcggta
     4861 tttggcgtag gcttgataga atacgctcga ttttctggtc actcagcatc agcggatcac
     4921 cgcctgaaat gatgacatct cgaatttcag gatgttctgc aatgtattta attccttgtt
     4981 gaataaagcg cggactgatc ttctgtgcgt caccgacctt gcgtttacgg gtacaaaaac
     5041 ggcaatatga ggcacattcc ggagaaacaa gaaaaagaca gcgatcggga tagcgatgga
     5101 caatactggg taccggcgaa tctttttctt cgctgagcgg atcgtattcc aagccgcaat
     5161 cttccaattc agccttgtca ggtataacct gcctgtaaat cggatcgcct ttctttttaa
     5221 tcaatgaaaa ataatatgga ttgaccttga tccgaaagac tttgtcaagc ccagccaact
     5281 cgtcatttgg aaggtttaag gctttggtga tttgatccac agtagtgtaa cttttttgga
     5341 ccaatttgcg ccatttttcc atttcttatt tattctcctg cttaaagtat tttacaaaaa
     5401 ttactcggtc atcaccccgg cgataaaaat ctttaatttg ggcggcaatt gcataaccat
     5461 ttttcagata aaaattgcgg gttggtaaat atttctcctg ggaggatgtt tcaattataa
     5521 tcagattgcc atgctgggct tggacagcgg tttctacata agtcaacaag tctgtgccga
     5581 tgcctttgcc ctgaacctgg ggagccactg caatccagta aagatcaaaa gtaccctcag
     5641 tagcaggggt aggcccatag caaacataac cggcgagttt ctggccattg atggctacaa
     5701 atattaaata atcagtttgg ttaggttttg tcaaagcggt gtcaatcaat tccatagcca
     5761 ccctaatttc cgccgtagta aacatctggg tatcgcttaa tattttcaga atctcggctc
     5821 gatcggtgga ttttatctcg cgaatcatgt tcatcttctt cgcctgttca aagcctgttt
     5881 tataagaaaa acaacaaatg tttcgtaact gataccggcg gcatttaaag cccgaacaaa
     5941 accagcagaa ggcgaaatat caggattggg attatattcc agtacaaata aattaccggc
     6001 ggcatccata cgaatatcaa ttcgtccata atctcgacca cgcaaagcgt gataaaccgc
     6061 taaactaagg ctagtcaatt tttcgttaag ttcggcacta atcggagccg gacaaaccgg
     6121 tggggtcatt tgataaacgg ggtgatcact gagccactta gcttcgtagc tagtgatggg
     6181 aaaatactct tcgctgacct gttcgaaatt aatttcagaa ggtggcaaaa ccagtggatt
     6241 ttcattgccg agaatactga cattgaattc gcggccggct atatattttt caaccaaaat
     6301 tggttgctta tacttagcca gtaactctcc gacgatcgac tttaattcaa tcagatcatt
     6361 gacgactgaa ttctgagtaa taccgatact accatcttcc ctcgaaggct tagcaatcaa
     6421 tggaaaatcc agactgcagg tttccggaat agattcgtag acgtaaaaat caggcgtggg
     6481 aattcctatg gctttaaaaa tatttttcga taacacttta ttctgtgcca gaccaagcgt
     6541 tagaggagaa ttaccggtat aaggcaaatc tagcatctcc cagagtccgg cgacattcat
     6601 ttcctggtcg gtgcgacttt gccaaccttc acagagattg aaaattacat ccggctggaa
     6661 ggagttaatt tttattaaat cctcatcaat gtttaaaatc ggaagcattt cggctacgta
     6721 accgcaatta ttcagagccg tataaacggc ggcagcttca tctctaacgg cgatttccga
     6781 aataatgtcc ggatcaaaat ttgaatctac cgattgcaaa tcgttgaaga ccaccagaat
     6841 tttgatcttc tgagattcgg atgacattag gacaagccgt atctccgggc ggcgacctga
     6901 acgacttggt ttaacattgc aggataggta taaccggcag tgcgcgcggc tttgggaaaa
     6961 caggaattat cttctggatt aggcaagatc cccggcaaag gattgatctc taaaatatgg
     7021 gcgaccccat tttcatccag acggacatcc aatcggcacc agtctctaat atttaaaatc
     7081 cggcaagttc tacgaacaag tagttcgatt tgattcttca gattatcatc aatttgagcc
     7141 gggcaggtga aaatatttag cggtttttct ggccgatccc aaatccattt ggcttcatag
     7201 gagtagatcg gttgagcacc ggtcggcaat tgtgaaaaat ccataccgat aatcggcaga
     7261 acttccattt cggggctatt gcctaggatt gcgaccgtaa attccggccc agccaggtac
     7321 ttttccatga ttaccggttc attattatat ttagcaaaac aacttgcaat tacgtcatca
     7381 gcttgttctt ttgagtaaac caccgaatta tcaaaaatgc ctttgctact accttctgct
     7441 aacggtttaa cgatagccgg aaatttaaaa ttttccgcaa aaccatcgtc cccgggatat
     7501 atgacctgaa aggtagcatt cggaatgtga taatagctca aaatttcttt acaacgtgct
     7561 ttattcagac aaattgccag tgttaaagga tcggaaccgg tatatggtaa acccagcctt
     7621 tcgcagatta agggcacata ggattcgcgc caatcgccaa atagcccttc cgtaacatta
     7681 aaaacgatgt ccgggcggct ttgttctaaa ccgtgcgcga cctcgtcatc accttcaaag
     7741 ctgactacct gatgattatt taattggaga gcatctacga ttgcctcaat cgtttgaggg
     7801 tcatcaccct cggcaaaaag gtcgggagga atgtcttcgt cgtccggatc gacggcaaag
     7861 cgcttacgat actcctttct caaaccttct tttgtgctat acgttaatgc aattttcatt
     7921 ttttatggcg tgaaatttac atattatttt tgaaatgcaa gtaaaatatt tttataaatt
     7981 gaaatttctt taaaattcta aaaagcccaa tcctttgggg aatgctattt taggattaca
     8041 aattgttgta tagaaaaata tctccaacct aacgcgggtg ggcaatctca aaggctttac
     8101 ggacaacagg atagtattgc gggtctttca aaaaggtctc gtaagagaaa aatactatat
     8161 tttgaaattg attatttaaa gttaatatcg ttttagtcat agcctcgtat tgactctgat
     8221 tatagacact aataccgacc cagacattat ttttatttat attagggtca attttcctca
     8281 ggattttttg gaattgctta gtatctttgg tatagttcat caagaccacg aaatccagcc
     8341 agtcttgcct taaccaatat tgccagtctt gaaagaaatt tttacaggca tcgtccggat
     8401 cgggcttgac ggccgcactt aattgtagag acggtcgaat ttctcgtaaa ttcttgcggg
     8461 cattatgaac gaaatcagaa atggcatctc gccgaaagtc ttcccatttg agcattaaag
     8521 aatcaatttc cgccagttct aatcctttat aaagtgattt gtcggagagg gttaagagga
     8581 gaggatcaat tccatatttc tcacgaaata atatgcggcc aataggattg taatcgtagc
     8641 aattttttgc ataacgaacg taatcaagat gaatgccatc gaggtcataa ttggtggcta
     8701 attcctgaat aatatcaatg aggtatttac gtacttctgg taagaggggc gataaataga
     8761 ttccttcggt acccaaggtg ttgaaatcag tggcacggcg ttggatatct tttataccat
     8821 tggcatctac cgtacaccac tcgggatgtt ggtacaaaag gtgattttca tttttcggtt
     8881 tctgatcaga tgaccatagc aaaaatacat taacccaggc atggactctc aatccataac
     8941 tatgggcttg gctgagagtg tattccagtg gatcccagtt attgttgctt agcaactcac
     9001 tatagactac aatttcggag tgataaaagg catcaccgcg accacgaact tgaacaaaca
     9061 gatcttttat atcatttaga acggcgaagt taacgaaggc atctattttc tctttagaat
     9121 tgagctggtc gcgaattacc cataagccga ggttggtctg ggctcgagcg gtgaccgtta
     9181 gactataaat tagaagggca gtcatcgcga ctgccctgat ttttaaagat aattgatttt
     9241 tcaaaaacaa tccaacaagt ttatttactc agtctccgat gcgggttcgg ctttttcgcc
     9301 tgatatattt tctggtggga tatcttcttt tacttctgct tgggctgatt gtttttcttc
     9361 ttttttggcc tgagtttttt tggtaggctt ggtcttagct ggagtttgtt ctttttctac
     9421 ttctgttgat ttttcctctt tcttcccctg aactgattta gtcgcctctg aaccggcctt
     9481 cttctttttt tcttccggtt gtttataatc tacaaattcg attaaagaaa cagccgcatt
     9541 atcattatcg cgatagccta gtttaataat tcgagtataa cccccggtgc gatttgcata
     9601 ctgcggagca atgtcttcga ataatttatt tactaaatcg cgatcctgaa taaatcggaa
     9661 agcaaggcga cgggaatgga cagtatctct cttgccataa gtaatcaagc gttcaactac
     9721 gcgacgtaac tcttttgctt ttgcgtcagt agtttttatt ctaccattta tcagcattgc
     9781 ctttgttaga ctttgtatta gcgccttgcg ctggtcagtt ggtcggccta atttagctac
     9841 ctttttctgg tggcgcatat cagtggtact ccttttgggt tattcttctt tctcaacgag
     9901 gtatttgtca acatccatgc cgaattccaa gcccatgctt ttcaatttgg tcattaactc
     9961 atttagagat tttcgtccaa aatttttgaa ctttagcata tcggcttctt catgggaaac
    10021 cagatcggca atggtacgga tgttattggc ttttaagcaa ttgtatgatc taacggataa
    10081 ctccatttca tccacgctct ttttgagcag attcctgatt ttaaggacat cttcgctggg
    10141 ttcttcacgt tgaaattcga tcggtttagc ttcgacgcgt gaaaaaaggc taaagaaatc
    10201 gcgtagaatt ttagaggcta gatcgaggga ttcatcggga gtaatgctat catctgtcgt
    10261 aatttccagg gttagtttct cgagaatttc acgggcggta ccgggcacat tttcgacttt
    10321 ataggataca ttaagaatgg gtgaaaaaat cgaatcaatg aaaatcgttc caatcggata
    10381 atcgggcagc ttattcttct ccgctggtac ccaacctgtg cctcgggcga tcctaatctc
    10441 aatattgaaa tcggcatcct cattcatcgt acagatatgt aaatccggat tcaaaatctt
    10501 aaactgggta atattttcgc caatcattcc ggccgtaaat tcaccagcgc ctttaaaatg
    10561 gagagtgact ttatctggct tgggatcgaa tagtttgagg cgaacttgtt tcagattaag
    10621 aattatttcc gcaacatctt cacgtactcc aggaatagtg gaaaattcat gcaaaacgcc
    10681 atcaattttg atcgaacaga ttgccgcacc cggtatcgaa gtcaagagaa cccgtcgcag
    10741 ggcattaccg atggtgatgc caaaaccccg ttccagcggc tgtagataat aaatgccatg
    10801 agtgtcatta gaattttcgt ctttaacgac tctggcgatt atttgatcgt aaacagtcat
    10861 tatttccctc cttatttaga ataaagttcg acaaccaatt gttcgttgac atcaacgttc
    10921 atctgatctc tttccgggac ttggatgaaa attccctgca ttttggcttt gtcaagaatc
    10981 aaccagggta gttcgtgctc gccacggatt cgtttcatcg aatcatgaat tacctgcaat
    11041 ttcttgcttt tttctctaac ctgaatgaca tcaccagctt tgagttgata ggaagggaca
    11101 tcaacgacgc ggttgtttac ggtaaaatgg cggtggctga ctagctcgcg tgccatccgc
    11161 cgggatgaag caaagccaag ccgatagacg acattatcca gtcttctttc cagaatcttc
    11221 atcaggttag taccggtgac gcccttctgg cggttggcta attcgaaata ttggcggaac
    11281 tgtttttcta gaacgccata aattctcttt attttctgtt tttcacgtaa ttggattcca
    11341 taatttgaca aacgtggatg agcagtctga ctgccatgtt gaccggggat ataacttttc
    11401 gtttcaaaag agcatttggt gctgttacag cgttcgcctt tcagaaaaag ttttttaccc
    11461 tcccggcgac atagtttgca tactggacct gtatatcttg ccattaaata ttctccattt
    11521 tagaaattat actcttctcc gctttggagg ccggcaacca ttgtgcggta ttggggtaac
    11581 gtccttaata gctgaaattt gtagtccggc taaatttagc gctcggatag cggcttctct
    11641 gcccgcgcct gggcctttga ctcgtacttc gacgcgctgt aatccaagat taatggcttc
    11701 ttgagcggct tttgtggcgg cttgctggga agcgtagggc gtgttcttgc gcgagccttt
    11761 gaaaccggct gttccagctg aagcccagga aattacatta ccatagttat cggttagtga
    11821 tattatcgta ttgttaaagg ttgctttaat agtagcaatt ccttccgatt caacttttgt
    11881 gcgtttcttc ttttttacgg tagaacgttt gggtggcaaa tcaaatacct cctattttat
    11941 ccttttttcc cgacttttac tttcttacgg ctaactgtgc gccgtggtcc tttgcgcgtg
    12001 cgagcattgg ttttagtccg ttgaccgcga accggcaaac ctcgacgatg gcgcaagccg
    12061 cgataacaac caatatccat taggcgtttg atgttcatct gaacttctgt gcgcaaagta
    12121 ccttcaactt tatattcatt gctaatcaca ttgcggatat cgcgcacctg ttcttcggtt
    12181 agcgctttta cccggatttc aggatcaact cccgctttct gcaggatatc catagcatta
    12241 gcccggccaa ttccatagat gtatgccaag gctattttca ctttcttttc gttcggtaaa
    12301 tctacaccgg caattcgagc caaattttcc tcctaattgc ttttatccct gtcgctgttt
    12361 atgttttgga ttggtacaaa taactcgcac tactcctttg cgacggataa ttttacattt
    12421 gttacaaatt ttcttaactg atgatcttac tttcattttc tatatccaat cttcatttat
    12481 agcgataagt tatgcgaccc ttggtcaaat catagggtga tagctccatg gtaaccttat
    12541 cacccggtaa aatcttgata aagtgcatcc gcattttacc ggaaacgtgc gccagaactt
    12601 catgtccatt ctcgagcact acccgaaagg aagcgttcgg caaattttcc ttaattattc
    12661 cgtcaacttt tatcaatttt tcttttacca cttttttttc cttttatttt ctttgtgata
    12721 aaatttgcgg tccatccgga ccaatagcaa tcgtatgttc gtaatgggct gaaggtaaat
    12781 gatcctccgt gattaccgtc caaccatctt tcaacaaacg gacttcataa cttcccatcg
    12841 taatcatcgg ttcgattgcc agacacatac ccggtttcaa aactggtccg cgacccggtt
    12901 caccgtaatt gggaatctct ggcggctcat gcagttcgcg acccaccccg tgcccaacca
    12961 gttcacgcac aactccataa ccgtagctct cgacgtgggt ctggatggca tgtccgatgt
    13021 ccgataaatg atttccgggt cgagcctgag ctatgccttg atagagtgaa tcctccgtga
    13081 cgcgcattaa ttgtaatcgg tcatcagaga ctgttcccac ggcaaaggtc atcgccgcat
    13141 cgccatagta gccatttttc ttgactccga cgtcgatgct gataatttcg ccctctttga
    13201 gaactcggtt ggaaggaatc ccgtggacaa cttgctcatc aatgctggtg catatattgc
    13261 cgggaaatcc gttatagccc ttgaaagccg aatgcccgcc ttggctctta ataaattctg
    13321 ccgccagatg atcaagatag agcgtcgtta taccagactt tatctctcga cttaacaaat
    13381 cgagagtttc agagacaatc cgacaacttt cacgaatctt ctcaatttcc tcggctgtac
    13441 gaatatgaac cattatcgct aattatccgc ggcggccctt taattttccg cttttcaaaa
    13501 aaccatcata atgtctcatc aaaagatgag attctatctg ctgtaatgta tccaaagcta
    13561 caccgacgac gatcaggaga cttgttcccc caaagaaaga agccagatca taattaatgt
    13621 ccataatctt catcagtata tagggaaaaa cagcaatgaa tgctaaaaag atcgagccgg
    13681 gtaatgtgac tttggttaaa atattatcaa taaactccgc cgtcttttta ccaggtctaa
    13741 ttcccggaat gaaaccacca tactttttta gattatctgc gacttctacc ggattaaagg
    13801 caatcgctgt ataaaaataa gtaaaaaaga cgattaaaac tccataaatc agccaatata
    13861 caattgattg cacactaaat attcgcatca ttccctggac gaaattactg ttcggaaaga
    13921 aagtagcaat agtgctcgga atgaatacaa tcgactgcgc aaaaatgatc ggcattacac
    13981 cggcggtatt gacccgcaga ggaatgtgag tcgcctgccc tccgtaaact ttccggccaa
    14041 cgactctttt ggcatattgt actggaatac gacgtgtgcc ttgggtcaat agcaccacaa
    14101 atccgacaat tactatcatt aatacgatta aaattatttc cgtaaataat ccgcggacac
    14161 cttctcgaat cagagtaatt tcgctgacaa acacgtttgg taaacgcgcc aggattccga
    14221 ccattataat catagatgta ccgttaccaa tacccctttc tgtcatacgt tcaccaagcc
    14281 acattagtaa aaccgtaccg gttgttatag tcagcattgt cattaaaacg aaaccaactc
    14341 ccggtcgggg tacaactggt agcatttgat tgcccacgcg ggctgacatc tgaggactca
    14401 tcagaaattg agctactcca aatgattgca gggcggcaat aataaccgta ccataacgcg
    14461 acatctgggt caattttttt cgtccttctt caccctcttt ttgtaacttt tgaaaatatg
    14521 ggattactgc accgagcaat tgtaagataa ttgaagccgt aatataaggc attataccca
    14581 gcgcgaaaag agtcgcctga gcaaaagccc ctccggaaaa catatcatac agtccaaaga
    14641 gtgtattcgc gaaactactc attgccgcac ctagtacttc agtatcaata cctggaatcg
    14701 gtatatgggc tccaattctg aaaactatca gaagggcaag ggtataaccg atacgttttc
    14761 tcaattcggg aatcttaaat acactctgga attgctggat cataaaagtt ctacctttcc
    14821 tccggaagcc gttattttct ctttcgctcg ggtactgaag gcatgcgcaa cgacggtaat
    14881 agcaccagag agttcacctc gccctaatat ttttaccggt ttttttaggc ttttaatgag
    14941 gcctttttgt tgcagaattt ccggattcac ttcggttatt tgtaaacgag caatatgatc
    15001 caaattgact acttgaaatt cttctttgaa taaattttta aaacctcttt tgggtaaacg
    15061 gcgatagatt ggcatctgtc caccctcaac atagacgggt tttttattgc taccccgtgc
    15121 accagttcct ttattaccgc gaccggctgt accgccgtgt ccggaaccag gtccacgccc
    15181 gaggcgcttt ttatttttta ctgaaccgta agccggttta agtgtgttta agcccatatc
    15241 ctattcctta atctcttcta cttttagtaa atgcggaatg gagttgatca taccgcggat
    15301 tgccggattg tctggtaaaa gaaccgtctg attcatttta gtaattccta aggcaatcaa
    15361 ggtgcgtttg gttttttcac agtagccgat tccgcttttt acctgagtaa ttttcagcat
    15421 tgcagtttta tctttttttg tcatgaacca aatatctcct gaagcgtttt ttcgcgtcta
    15481 tgagccacca tgtaggggtc ttttaatttc gagagggcaa gcattgtcgc tttgactaca
    15541 ttatgcgaat tattagagcc gagcgatttg gataaaacat cgcgcacacc gaggctttcc
    15601 ataatagccc gaatcgcacc cccggcgata acgccagtac caggagtagc cggacgcaga
    15661 atgactttgg cggcgccgta tttagcctgg acttcgtggg gtatggtacc gttgatgacc
    15721 gggattttta ccaaattttt cttggcgttt tctttgcctt tagccaccgc tgaactcacc
    15781 tcgagggctt tgcccagacc aatcccgacg tgccctttgc cgtcaccgat tgctaccagg
    15841 gcattgaagc gaaaccttcg tccaccggta acaacctttg caactcgatt aatttttatg
    15901 actttttctt ccagtaattc cagttgagtt ggatctatag attttctcaa gttaccattc
    15961 ctccgctatt agaattctaa tccggcttga cgtgccgctt ctgctaaagc ttttacgcga
    16021 ccatgatatt gatacccgct ccggtcaaaa actactttag taataccttt tgccacagct
    16081 ttttcggcta aggttttacc caccagagca cttcggtcgg actttttagt attgcttttg
    16141 atctgctcgc ggacttcagg cgtaaggtct gaaacggcga gaagtgattt atgaaggtta
    16201 tcatcaacaa cctgggcata aatatgattc aggcttcgga aaaccgtcag acgcggccgc
    16261 tccgacgtac cttgtatttt gtgtcggtta cgtttctggc ggcgctgtcg agcgaatgct
    16321 tttatattct tgactttatt catgtataaa acctctattt cacaccaact tttttaccgg
    16381 cttttctgcg gatatgttca ttctggtaac gaattccttt acccttataa ggttcacacg
    16441 gtttgataga tcgaattttt gccgcaattt gtccgacttt ttgtttatca atacctttta
    16501 cgacaatagt aagggctttg ggaacctcaa agcttacccc ttcgggaggc gtaactaaga
    16561 ttgcatggga ataaccaaca ttaatttgaa cggatttgcc cttcatctca gccgaatagc
    16621 cgacaccttc gatttccaga gtcttgctga atccttgcaa tacaccctgg atcatattag
    16681 cgatcattac ccgcgtcaag ccgtgtaaag ctttatgatt gcgactatcg ctcgggcggg
    16741 taacaattat ctgactatcg tttatctgaa ctttcatatc ggacggaaaa tcccacttta
    16801 gttcaccttt gggacctttg actgtaatgc tacaatcttg gattgtaaca ttgacttctt
    16861 taggaatttc gattggtttt ttaccgattc gtgacactct atactccttg aatcaccttt
    16921 ttaccagaca tagcaaagaa cttcaccacc cacattattt tttcgggcga ctttatcggt
    16981 caggacgccc attggagttg ataaaatagc tattcccatt ccaccgacta cccgagggat
    17041 attatccgaa gccgcataaa cacgccgacc aggtttgctg attctttgca aaccttcgat
    17101 catcggttca ccagttttag aatagcgtaa gtagatacga atgagtccct gtttgccgtc
    17161 tttgattaaa ataaagtcct tgataaaatt ttcgtgcttt aaaataagcg atattctctt
    17221 ctttaaattg gaggcaggaa tatcaaccca gcgatggttc gcggcttggg cattacgaat
    17281 acgagttaaa taatctgcaa ttggatcggt taccgccata tcatatactc ctgtattacc
    17341 agcttgcctt tgtgataccc ggaatttcac cgcgcagggc taattcccga aaacaaagcc
    17401 ggcaaagacc aaatttacga taataaccgc gcggacgccc gcaaatcgaa caacgattga
    17461 ctctccgcgt actgaatttg ggatttcgcc tggctttggc aataagtgat tttttagcca
    17521 tcttagttta tctccattgc ggtcttgact tcaccgccgg ttgttgattc tttgattgct
    17581 cttttcttga acggcaaccc gagaagggat agcaattcat acgcttccgt atcggtttta
    17641 gccgtggtag taaaagtaat gttcattccc cgaatatgat caactttatc ataatctatt
    17701 tctgtaaaaa taatctgttc cttgagacct atcgaatagt taccgcgacc gtcgaaagat
    17761 ttatcagaaa gtcctgaaaa gtcacgtact cgtggtaaag ctgtcgaaat aagccgatcc
    17821 ataaattcat acattcgaaa acgacgcagg gtaacggcac aaccgaccgg atctccagtt
    17881 ttaattttaa aattggaaat ggcgcgtttt gctttagtca ccatcggttg ctgtccagta
    17941 ataatacgaa tgtcttcaac tgctttcttc aagctagctg gatcttcctt ggttttgccc
    18001 actcccatat ttatgcttat tttgacaagt ttgggaacct gcataatatt tttataaccg
    18061 aattttttca ccatagctgg aactatttcg gtctcgtatg cggtcattaa gcggggttta
    18121 tatgcagact tttttgccac ttatagctcc taattatata gcatcaataa tttcaccagt
    18181 ttcgcgtgct acgcggactt ttttcccatc tttcagagtc cgatagccga ctttagttgc
    18241 ctgatcggca tgtactaatt tgacattaga tatatggatc ggcgcttctt tttcaataat
    18301 accaccttga ggattttgct ggttggggcg agtatgcctt ttgataaaat ttactccttc
    18361 cacgattact cgttctttat ccgggaaaac tttcaaaacc tttccgattt tgcctttgga
    18421 ttctcctgtt ataaccatta ctgtatcatt tttacgtatg tgaagcattt ttatattcct
    18481 ttataatacc tcgggcgcca tagagacaat tttcatataa tttttctcgc gcagttctct
    18541 ggcaactggc ccaaagattc gggttcctct cggctcaagg ttattgtcaa tcagaacagc
    18601 ggcattgtca tcaaagcgga tatatgagcc atctttgcgg cgaacttcct tggtcgtgcg
    18661 cacgacgacg gctttggatt tttcgccttt tttaaccata ccgccgggcg tagcctgttt
    18721 tatggttaca acgatggtat caccgactgt tccgtattta cgatgggaac cgcccattac
    18781 tcggatgacc attacttctt ttgctcccgt attgtcggct acttttaatc ttgattcttg
    18841 ctgtatcata gaaatatcct ttgtggtttg tcctagcgtt caattatttc cagcagacgc
    18901 catctcttca accggcttaa gggacgcgat tcgacaattt taactttatc gccttcgtga
    18961 catttattct cggcatcatg gacataaaat ttggaagtcc gtcgaatata tttgccataa
    19021 gtgctgtgct ttacagttcg ctcgacggat acgacagcgg ttttatcttg cttatgactg
    19081 atgactgttc caattaaaat ttttcttctg tcgcttgaaa tcattcttga ttgtcctgtg
    19141 atttatttat tcggtttatc ttttgatctg attcccagtt cgtactcatg taaaacggtt
    19201 cgaatttgag caatttctct cttgacgtgc ctgatcttca gtggattttc taattgctga
    19261 agagcttttt gaaagcgtag gttttccaag gcggttaaat tgtcgcgtaa tttagtttcc
    19321 agttcattca ccgttaattc atcaagagta ctttttttca ctttatgctc ctaattctct
    19381 tctagctacg actttggttt taatgggcaa tttatgggta gcagtcttga atatgtcttt
    19441 agcttcttgt tcgctggcta cttcaatttc gaataaaata cgtcccggtt ttaccacggc
    19501 gacccagaac tcaggtgtcc ctttgccttt tcccatgcgg gtttcagctg gttttttagt
    19561 gataggttta tccgggaaga tccgaatcca cattttacca tgtttgcgaa tttggcgcga
    19621 aattgctact cgaacagctt cgatctgccg actatcaatc cagccgggtt ccatagcttt
    19681 taaaccataa tgaccgaaac tgacggcatt cccacgggtt gcgattcccg tcatccggcc
    19741 tctttgctgt tttctccact ttatcttttt cggtgccagc atgattaatt attccttttt
    19801 taacttttcc tgaatctctc gccgttacaa atccagactt tgattccgat gcaaccgtaa
    19861 gttgtatgcg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgcttgatt
    19981 tcattaacat tgatctggac atcggttttg gtgactttct gtaattcgag tcggagtaat
    20041 tcaacttctt cgcctttttt gccaataaca atgccgggtc gcgaggtatt aatggtaatt
    20101 gtgatttttt taggagtccg atagatatcc actcttgaaa tgccggcatt aggaagacgt
    20161 ttcaaaacat aacgccgtaa tttgatatcc tctgttaatt tttcagtgaa atttctttcg
    20221 tcaaaccagt aactgctcca ctccttgttg aatcccagac gaaaacctat tggatgcgtt
    20281 ttttgaccca agtcacctcc gattattgta ctttactttt ttgtttattt tctttttccg
    20341 ccacaactac agtaatatga cagagccgac gcctgatgat actggctcga cccagtgatc
    20401 cagcccgaaa gcgtttagca atcgagccgc catctacgta gatagtcttt atatagagat
    20461 cttccggatt taatttcgaa ccgccctcac tattcaagag gttggacact gcggaccgaa
    20521 cgaccttttc tatggtctcc gaggcttttt tactattaaa gtgtaggagg tttaaagccg
    20581 tttcaacgtc tttcccccgt actaaatcag caaccagacg gactttccgc gctgattgcc
    20641 ttacatatct atgaactgct ttggcttcca taattcgatt taggctccct tcttttccgc
    20701 accggaatgc ccgcggaaga ttctggtatg ggcaaattcg cccagcttat gacccaccat
    20761 attttcggta acgaagaccg ggatgaattt attgccgttg tgtacggcga aagtgtgacc
    20821 gacaaactcg ggagagatcg tagtgcgacg tgcccaggtt ttaatcactc gtttttttcc
    20881 agagctattc atttgctcga ttttcttcat caatttaaca tcaatgtatg gacctttttt
    20941 cagcgaacga ctcatccaat attctcccta attatttagt tcgtctcttg ataataaact
    21001 tattgctggg attacgtttt ttacgtgttc gatatccttt tgataactgc ccccaaggtg
    21061 aacatggatg ccttccgccc gagctcttac cttcgccacc acccattgga tgatcaatcg
    21121 gattcatagc cacacccctt acatgcggac ggcgacccat ccagcgactg cgtcctgctt
    21181 ttccaatatt gatattttca tggtctttgt tacttacgac gccgatcgtg gcatagcatt
    21241 cttcattgat gagccgtatt tcacccgaag gcatttttaa agtcacatac ttgccttctt
    21301 tagccataat ttgagcggct gtgccggctc cgcgagcgat ttgccctcct tttttcggac
    21361 gcatttcgat attgtgtaca aaagtacccg cgggaatatt ttttaacggc agacaattac
    21421 cgaccttaat gggggcttct tcacccgaga ggacaacgct accggtaacg agtccatctg
    21481 gtgcaattat atagcgtttc tcaccgtctt tatagaatag aagcgcaatg cgtgctgatc
    21541 gatttggatc atactctata gctttgacag tcgcttcgat tccatatttg ttacgcttga
    21601 agtcaatatt ccggtaagca cgcttttgac cgccaccccg atggcgcgaa gttacccgac
    21661 cgtaattatt gcgaccggca gattgcgtaa ttggttcgag cagacttttt tcgggtctct
    21721 ttttggtaat atcttcaaaa gtatcgaccg tcttaaagcg ctgtccgggt gtaatcggtt
    21781 tgatagtttt aatacccatg atactatacc gccgtttctc cttgataaaa atctattttt
    21841 tcaccctcct tgagggttac aatggctttt ttccagtttg accgacgtcc agtagtgcgt
    21901 ataactcgac cgccactccg gactgtcatc agttttgttt tacccataat attctgagta
    21961 gcgacttttt ctactttgac cccaaactta tcttcaacag cctttttgat ttcgattttg
    22021 ttggcagagg gatgaacttt aaagccatat ttattttggc tttcctgtaa ttcgttaatt
    22081 ttttcggtta gaattggttt gataatgatt gacttagaag acatatttag ttcctactat
    22141 cgcccaacaa ggcattattt aacttttcga agccgctttt atcgattaag agaacttcgc
    22201 agtccagcag atcgtaggtc gaagcttttt cagcttcgag gactaagaca ttgtaaaaat
    22261 tgcggctggc taacaataga ttagtattta aattagcggt tagaatagtc acttttttat
    22321 ccaagaggtc aaattttttc agtaattcgc ggatatttct ggattttggc tcgtcgaagg
    22381 acaattcctc gatgattttt agcgcacctt cgcggtagcg gtaagccaga gcgctttggc
    22441 gcgccagtga tttgactttt ttgttaattt tcatcttata gggatgcggt ttgggaccaa
    22501 aagcccgccc accgccgacc cagaccgatg accgacggga gccagagcga gcccgaccag
    22561 tacctttttg tttccatggc ttcttcccac caccccgtac ttcaccacgg gttttggtag
    22621 aatgagtacc ctgacgacta ttggtcatct cggttttaac ggctaaataa atagcgtggt
    22681 cgtgcggttc gatatcaaag gctggggtct cgattactaa tttttggcca gaatctgaac
    22741 catctaattt ataaacagaa aattcgatac tcaccgttcc tatctcctaa tagttagaat
    22801 actgtttttt gaaccaggta cggcgccttt gatataaagc aagttgcttt cggggtcaat
    22861 tttaacaact gttagattat tgaccgtgca acgcttgccg cccaaacgtc cagccatacg
    22921 cattcccttg ataacatgcg atggataggc ggaagcgcct atggaaccgg gagcacgtaa
    22981 acgatcactc tgtccgtggg tttttgggcc accgccaaaa ttgtggcgtt tgacaacacc
    23041 ttgaaaacct ttacccttgg aaatgccggt aattttaacc ttatctccag gattgaagag
    23101 cgcaacagtt actgtttcac ctacctgaca aaatttagga tcgtaatcac gaatttctac
    23161 cagatacttc aggggagtaa tattattctt cttgaaaacg cccagattag gcttagccat
    23221 tagacgttct ttggcttcca taaatcccag ttgaaccgag gcataaccgt ctttttcatt
    23281 cgttttcacc tgaaccactt tgcaaggccc agccgcaata actgtcaccg ggatcaattc
    23341 accattctcc ttgaatatct ggctcatgcc aatttttttt ccaattattc cgatcataat
    23401 gtatccattt ataccttaat ttcgatgtcc acaccagctg gtaaatccag tttcatcaga
    23461 gcatcaacag ttttggaagt ggagttcaga atatcaatca gacgcttatg aatcttggtc
    23521 tgaaattgtt ctctggattt tttatccaca tgtggagaac gtaaaaccgt atataccgtg
    23581 cgttgggttg gtaatgggat cggtcccgag accatcgctc cggtggattt agctgttgct
    23641 acaatctttc ttgtactttt gtcaagtaaa ttatgatcgt aggcttttag actgattctg
    23701 attctttgcc caggcacagt tttactcctt attcaatgat ttcggtaacg acgccagcac
    23761 caactgtccg tccaccttct cgg
//
LOCUS       DAOOMU010000027        23758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2021_length_23758_cov_22.871662, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000027 DAOOMU010000000
VERSION     DAOOMU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 23758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23758
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2021_length_23758_cov_22.871662"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1957)
                     /locus_tag="PLI55_04040"
     CDS             complement(44..1957)
                     /locus_tag="PLI55_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HPB00041.1"
                     /translation="MEVTQKIGLLDWAVVAIYFALIFGIAIYYYRRAGENTGEFFLSG
                     RNLPWWLAGTAMVATTFAADTPLAVTELVAKNGIAGNWLWWNLAIGAMLTVFFFARLW
                     RRAGIMTDVEFTEMRYSGKTAAALRGFRALYLGIFMNTIVMGWVHKAMEKIFHVTIPG
                     IDPFLLVAIVAGIVAIYASASGLLGTARTDSFQFVFAMTGCIILAVIVIRLPEVGGAV
                     KMKAQLAPQVLDFFPKVGRINGEGITGGVLSLSLGAFIAHIGLQWWSSWYPGAEPGGG
                     GYIAQRMMSAKNEKHSLLATLWFTIAHYTLRPWPWIIVALSALVILPRASSTDILQQE
                     NPVLYHQVVEAYKNPDLMKSNPLASTPEFQNFYEKYENTVDPGVMYPKMMLRYLPTGI
                     LGLLIATFLAAYMSTISSQLNWGTSYIINDFYRRFLRKKASEKHYVLVSRIAMLIMIV
                     FSLFVTKYFLTTISGAWEFIINASAGMGAVLILRWFWWRINAWSEISAMIAPLIIYPI
                     ARYKFGLQSPITLYPVVLGTTIVWLLVTFLTKPEKDSTLTSFYRKVHPGGSGWKKIAE
                     QLPDVKGDSGFGRAFICWIFGVLLVYASLFGIGKIIFHEYLLAGVYFLVAGISVWIII
                     HNLNAVGWKTLKE"
     gene            complement(2188..2925)
                     /gene="rnc"
                     /locus_tag="PLI55_04045"
     CDS             complement(2188..2925)
                     /gene="rnc"
                     /locus_tag="PLI55_04045"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPB00042.1"
                     /translation="MKVSVKNWFRKISPIEKEIGYVFRNKALLEQALTHRSRSVNGEK
                     SYERLEFLGDAVLAFIVAEILFEKYPDKSEGELTMHRSALVNGNALARVGSKLGIEKA
                     VIVEKGLNLKNPPTLHKLLASIVESLIGAIYLDRGLRPARKFVQRWIIPSTAEGKVVS
                     NFNYKGRLFEYCQKNGRNLPVFKVLRVSGPDHAKKYEVAVYINGQKMGYGVGRQKRNA
                     EQKAAHEALQKLMPEHTVAGNQILKDS"
     gene            complement(2922..4169)
                     /gene="fabF"
                     /locus_tag="PLI55_04050"
     CDS             complement(2922..4169)
                     /gene="fabF"
                     /locus_tag="PLI55_04050"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPB00043.1"
                     /translation="MPKRVVVTGMGVISPVGSDVPSFWSNLLNGVSGVDYIKSFDASG
                     YSTQIAAEVRDFDPLKYFTQKDINRMDRVTQFGSAAAKQAVYDSGLIESGFSAERVGV
                     IVGSGIGGISTLEAQHQILMTKGHRMVSPLFIPMMIPDIIPGQIAMDFGFCGPNYGVT
                     SACATGSHAIGVAYQHICNGTADVMITGGAEAAITPLAIAGFANMKALSRCNNAPQKA
                     SRPFDSKRDGFVVGEGSGIVVLEELEHALNRKAKIYAEIVGFGFSADAYHITAPHPEG
                     KGAIISMENAIRMSGLPKSAFGYINAHGTSTPLNDRNETIAIKAVFGKQAYQIHISST
                     KSMTGHLLGAAGGIEFITLALALQNQIIPPTINYEFPDPECDLNYTPNIAQKATLVAG
                     LSNNFGFGGHNAVLAAKVYKPEN"
     gene            complement(4178..4411)
                     /gene="acpP"
                     /locus_tag="PLI55_04055"
     CDS             complement(4178..4411)
                     /gene="acpP"
                     /locus_tag="PLI55_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012513553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPB00044.1"
                     /translation="MSNFEKVQEIVAEKLGIEKSKITMEASFIDDLGADSLDTVELIM
                     KMEEDFGIEIPDEDAEKLKTVGDVVNYLDSKLK"
     gene            complement(4431..5177)
                     /gene="fabG"
                     /locus_tag="PLI55_04060"
     CDS             complement(4431..5177)
                     /gene="fabG"
                     /locus_tag="PLI55_04060"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HPB00045.1"
                     /translation="MELTFKDKIVIVTGSGRGIGLTIAQTFAENGATVILSDYNQESL
                     ADAVALFKAKNFPFDVLPCDVTNSTEVQNLIESIIQKYTRIDVLINNAGVTRDAILLR
                     MKEEQWDAVINTNLKGVFLVCRAAAKYFLKQQSGKIINISSVVGLTGNIGQSNYAASK
                     AGVIGFSKSLAKEMASRNITVNVIAPGFIDTAMTAALSDEVRTQFLRNIPLNRMGTPQ
                     EVANAALFLASPLADYITGQVLKVDGGMVM"
     gene            complement(5168..6094)
                     /gene="fabD"
                     /locus_tag="PLI55_04065"
     CDS             complement(5168..6094)
                     /gene="fabD"
                     /locus_tag="PLI55_04065"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPB00046.1"
                     /translation="MTKVAFIFPGQASQFVGMGRDFYESSALARQYYDLAETRLGFPL
                     KKFSFEGPLSELTKTRITQPAIFVLSCIIFFELKARGYLPDMVAGHSLGEYSALVACG
                     ALTFEDGLDLVKIRAEQMQIASEQKPGTMAAIVGLDYDQVKKIISESNFSGICQIANC
                     NSPNQIVISGDVYKVQALMTDLKKAGAKMAVELSVGGAFHSDLMQPASEALKEALSKT
                     EFKTPDCPIYSNVTATATRDPQEIRNRLYQQLTSPVLWTDTIRNMIADGAEIFVEVGP
                     GKVLQGLVKRTSADVNVTGVSKFSELEQIAWN"
     gene            complement(6087..7094)
                     /locus_tag="PLI55_04070"
     CDS             complement(6087..7094)
                     /locus_tag="PLI55_04070"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPB00047.1"
                     /translation="MKKPKSTITAFGMYVPERILTNADLEKMVDTTDEWIRTRTGIRE
                     RHIAAEGEATSAMAIKAFQDLQRRFNVDPLTIDLIIVATISPDMFFPSTAALIQEGIG
                     AKNAYGFDLSAACSGFIYALANAAQFVENGTCRRVLVFGADTMSSITDYTNRDTCVLF
                     GDAAGVVLLEPTPPGDDSGIIDFILKMDGSGKDYLYMLGGGSLHPATAETVAKKMHYI
                     YQEGKTVFKFAVKSMADISLEVLERNGLTGKDIKLFIPHQANKRIIDASADRLKLSDE
                     QVMINIDRYANTTAATIPLALVEGFDQKRIKRGDLVLMAAFGGGFTWGSLLLRWGIPN
                     D"
     gene            complement(7196..7378)
                     /gene="rpmF"
                     /locus_tag="PLI55_04075"
     CDS             complement(7196..7378)
                     /gene="rpmF"
                     /locus_tag="PLI55_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006905007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPB00048.1"
                     /translation="MPQPKRRASKSRRDKRRTHQVLTEATVVKCPQCKEPKLPHRACP
                     NCGYYRGRPVIIPKEK"
     gene            complement(7380..7892)
                     /locus_tag="PLI55_04080"
     CDS             complement(7380..7892)
                     /locus_tag="PLI55_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPB00049.1"
                     /translation="MKVRINSLKYGKQMIRVALGGDELDVADLDLSAPVAVMLNIDRG
                     TTEIKIDGKVESIARFDCDRCLESFSMPISGTFAIIATYSESNVKIDDENVIQLSSMT
                     NEIDITTHIHDTLLLSIPMKILCEEDCQGLCPICGANHNKTECHCNVAEPDARWDSLK
                     KLTIKITEDQ"
     gene            8333..8893
                     /locus_tag="PLI55_04085"
     CDS             8333..8893
                     /locus_tag="PLI55_04085"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB00050.1"
                     /translation="MDFEEKCINSKRVFNGRLLQVYRDEVRLPNGKISNREWIKHPGA
                     AVVIPYLGNGQILLIKQFRYPVRQIMLELPAGKIDPDENPEQTIRRELAEETGYSAQK
                     LTKLSLIHTCVGYSNECLHLFWADELQKCDTRPDDDELIEIIPMSINAAMNLLYQGKI
                     TDAKTIIGLFWADNIINRRYLNLCSD"
     gene            9214..9855
                     /locus_tag="PLI55_04090"
     CDS             9214..9855
                     /locus_tag="PLI55_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008695721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPB00051.1"
                     /translation="MNFIWLGLMLIALIVGALNGRIELVMKAAFDNAAAAVEIALGLI
                     GIMALWLGIMKIAEKGGIIKILSRAIRPIAKFLFPSIPSDHPAIGSMLMNMIANWLGL
                     GNAATPLGLKAMEELQSLNQFKDTATNDMVTFLALNTGTICLIPMTVIAVRAQLGSAN
                     PFEIIGTTIISSTCATIAGVTAAKLFQRLPSIRKSDPNRLIKPNSDGEGVKHA"
     gene            9926..>10444
                     /locus_tag="PLI55_04095"
     CDS             9926..>10444
                     /locus_tag="PLI55_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HPB00052.1"
                     /translation="MFVRIMQIFSNWLIPVLIFAILILAWRKKVKVYEAFIDGAKEGF
                     SVAIKIIPYLVAVLVAIGMFRASGAMDIFVALFSPITNLIGMPAETLPVALMRPLSGS
                     GALGLVTELMKQHGPDSFIGRVASTMWGSTETTFYVVAVYFGSVGVRRVRHSIAAGLV
                     GDAVGLIMAVIIC"
     assembly_gap    10445..10544
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10560..11912
                     /locus_tag="PLI55_04100"
     CDS             10560..11912
                     /locus_tag="PLI55_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPB00053.1"
                     /translation="MNPYIFREYDIRGVVADDFSDEVVELLGKGIGTYFREKDAKTIS
                     LSGDIRLSTPHLKSVLTKGLLSTGLNIIDLGIVPTPVNYYSMYVLPVDGAIQITGSHN
                     PPDMNGFKISLDKKAVFGDQIQHIKNIIEKGVFAGGTGKVSEKSLLEEYKQMIISKIQ
                     LARPLKIAMDCGNGAASLTAPEIFKKIGCQVTELYCEVDGRFPNHHPDPTVPKNLKDL
                     IAEVQKGGYDFGVAFDGDADRIGVVDDLGQIIWADYLMILFLEEVARPGVPVIFDVKC
                     SQALEEMIISYGAKPIMWKTGHSLIKQKMKDENAPFAGEMSGHLFMGDEYFGYDDAVY
                     VAARLARLIARGNEKISAKTARLPRYFSTPEIRLECKDDPTKFKIAEQAAAYFKSHYD
                     CIDVDGVRIKFGDGWGLVRASNTQPVIVTRFEAKTAERLDEIRDLVLNKLREFGEFRL
                     "
     gene            11995..12966
                     /locus_tag="PLI55_04105"
     CDS             11995..12966
                     /locus_tag="PLI55_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPB00054.1"
                     /translation="MSVDFGTKILRLKEEIDRELSKIDLSGISSELVQPMRFSLNAKA
                     KRLRPILVLLVGENLGASRKNLMPAALAIEIMHTFTLVHDDIMDNDTIRRGQQTVHVK
                     WNVNTAILTGDALMALAFRVLMQTKSERLDQIGTHFSQAMLEICDGQAFDLAYEKYDE
                     IDLTDYLNMIARKTGRLLGLACQLAAIIAGASPKVVNNLYLFGLELGQAFQIQDDLLE
                     LTADQAEMGKSLGSDLTAGKKTFPIIMALNELPAGQRADLLNFLKANAGNRRAIVQQL
                     TKRGSIQQTEAMIARLFKNARERLHNMPDQLQSDLINLIELIAGRQS"
     gene            12968..>13103
                     /locus_tag="PLI55_04110"
     CDS             12968..>13103
                     /locus_tag="PLI55_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPB00055.1"
                     /translation="MVRQTVKILNRHGLHTRPATVLVTLCSRFKSDIFLIYKNVRVNA
                     K"
     assembly_gap    13104..13203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13204..14627
                     /gene="ptsP"
                     /locus_tag="PLI55_04115"
     CDS             <13204..14627
                     /gene="ptsP"
                     /locus_tag="PLI55_04115"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPB00056.1"
                     /translation="WAYDAAMEWYENALLNTMNQFQQERLIDLQDIKKRMFRHLSTEE
                     QLDLPQIPERVIYVGERISPSELIQLHNQKILGIITRIGGMDSHSGILARAFGIPYLS
                     NVNEIDLIGKSHQVILDADREIVIVDAKPSVLKKYAVKLRELEIRRQRLNETIPPAIT
                     RDGIKIEILLNAGFLSEVKNINLSLIDGIGLFRTEYLCLERDDLPNEEEQFRAYRQIT
                     MAMQGKPTTFRTFDFGREKMMAILNMEMFQKDAVFDTWGGIRFCLDNPRILITQLRAL
                     LRASQFGPLKIMFPLVSNSDEILQVLEIYQQVQSDLEKEGHAFNRNIPLGAMVETKSI
                     LDELEKLTELLNFFSVGTNDLALFLLGSKRADTISKNYYHPKILQAINQIINVTQKKN
                     FPVTVCGEMASDPYAILGLLALEVRSFSMSVSALIDINDLIRMVNIQKISSLKENLLT
                     ADNVQQVYKLLRSSYKRITKNKM"
     gene            14692..15273
                     /locus_tag="PLI55_04120"
     CDS             14692..15273
                     /locus_tag="PLI55_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPB00057.1"
                     /translation="MIKVALNKIIFYPPSKGYALLLKEVGGERQLPIIIGVFEAQSIA
                     LAIEEVKMPRPMTHDLFVNILHDLGAKILSVTVSDLIDGTFYAKIEISLANSKKNLYI
                     DARPSDAIALAIRMKAPIQVSEEVMQEAGQYLNSKRKDSPEKDDSLPEIQNLDHLFEL
                     QNNLQSAIEEENYELAAKLRDQINRLKQELNIN"
     assembly_gap    15586..15685
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15686..16909
                     /locus_tag="PLI55_04125"
     CDS             <15686..16909
                     /locus_tag="PLI55_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HPB00058.1"
                     /translation="KYPSELAGKLYPKGIPIFPEEELTYLIEKFDVDECVFSYSDVPY
                     QKVMALSALVNAAGANFTLLGPKATMLKSTKPVISVCATRTGCGKSQTARKVIEILMA
                     QGLKVVAVRHPMPYGDLVAQKVQRFATLDDLKKYHCTIEEMEEYEPHIVRGNVIYAGV
                     DYEAILRAAENDPDGCDLIIWDGGNNDFPFFKPDLAITVVDPHRPGNELSYYPGEVNL
                     RIADVIIINKIDTANPEGIQTVSQNIAIVNPQATVIYAASPVRVDHPEIIRDKRVLVI
                     EDGPTLTHGEMQIGAGIIAARQYGARECVDPRPFVVGKLAETFQKYPKIGPLLPAMGY
                     SEDQIRDLEDTIARTDCDSVVIGTPIDLTRILKIKQPSTRVYYELQEIGTPNLNEVLS
                     DFCQRIKLNIQTKQI"
     gene            16906..17847
                     /locus_tag="PLI55_04130"
     CDS             16906..17847
                     /locus_tag="PLI55_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPB00059.1"
                     /translation="MNRTAVVALGGNAISPNNEKDTIANQFKNTRKSLQSVMNLIRQN
                     YHLALTHGNGPQVGNALLRVELTIDKAPFLPLGICVADTEGGMGYMIEQSLQNALIKA
                     QIPREVVSIITQVIVAADDPSINNPTKFVGSWYSYNEIKELAERFGWQIKEDGQRGWR
                     RVVPSPIPRKIINSRAIKHLVDLGAIVIAAGGGGIPVYIMENGLYEGFDAVIDKDLAA
                     AVLAHDIAAQELYILTDIDSVKINFGKPNEESIDLIHIPELQCLLAEGQFPLGSMGPK
                     ISAAIAFIQSGGERVIITSLEKAIESLHGDSGTTIIA"
     gene            17859..18275
                     /gene="ndk"
                     /locus_tag="PLI55_04135"
     CDS             17859..18275
                     /gene="ndk"
                     /locus_tag="PLI55_04135"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPB00060.1"
                     /translation="MSMTLAILKPDCVKRKLIGEVISFIEKEGFRIVCLRMIHLQPQE
                     AAEFYSIHRGKDYFEGLMNFMTSGPSVLMVLERENAVEHFRKVIGATDPKKAAEGTLR
                     RKYAENVRYNVVHGSDSEENAKKEIAFFFSTMEIIK"
     gene            18299..19063
                     /locus_tag="PLI55_04140"
     CDS             18299..19063
                     /locus_tag="PLI55_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPB00061.1"
                     /translation="MKKLSLTLTILLMLLSNCSKKPVVVTGVVGNPINLVPEIKKSED
                     TTGCTFKWGFIAKPAESIMDVLSFIPDSRSFSVSFIPDVPGDYELQFLSQDSNGQEKF
                     KQIIKCSILPDTAQSPSTDTVAGQMPPRDLSAPPPQYAQSYPGGENETTYSAMTQSQP
                     IKTGKNIQKIPGKYTIQLGAYKNYDKAEAELKKLRAHNLDTYIQKAYFKETNETWYRI
                     RTGTFDSYAEAKKVLNQLKARFPKENLWIDYVREDQ"
     gene            19084..20268
                     /locus_tag="PLI55_04145"
     CDS             19084..20268
                     /locus_tag="PLI55_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00062.1"
                     /translation="MKKLKFDVRDIFLTPRLALSGKKIWVHMVGLAAGWVIYTILAYV
                     ALLVNGQSLAEIWRMYHFFPILCCTTCQLPWYSWLIYGVGVVAWIIIFLLASVAVARI
                     TYKQLKGDEFFSSGDSFKYVKKHGVAAVMGPVSILMIIAFFVILAIIAAWLAKWPVLD
                     IIFLGIPYLLYFVVATFVVYTAVVFVVSLVLAPAIVGTAEEDTMETVFQSYSTLWAQP
                     WRLVLYEGIVGGLTVVATFIYGWALILGYRFFNFVFSASWLMDGKMSRIIEQASSYLF
                     GINSPLTPWISQFFNVYTPASLGIVKPLYLGTTEIITVVLITIALFIIAGTVVAYALS
                     NLSVGQALIYIILRKKKDDEDLIERKDEDELKEEEKSEEPASEETKAPESETPSSDEK
                     TE"
     gene            complement(20449..20976)
                     /locus_tag="PLI55_04150"
     CDS             complement(20449..20976)
                     /locus_tag="PLI55_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB00063.1"
                     /translation="MNTKTKAQYVNISILSKYEIFNSLSEKNIEHFCEVIEVLEYESG
                     DTIIKEGEVGDSLLLLLDGTVEISQALTLKTDMSGIDTREKSLIKLSSTARPFFGEMS
                     LFSDDDKRTATVKASTKCLVGRISRVDFFQICNSNPTIGNIVMQNIARVLTERLKQAN
                     QNVLKLTTALSLLIE"
     gene            complement(20973..22058)
                     /locus_tag="PLI55_04155"
     CDS             complement(20973..22058)
                     /locus_tag="PLI55_04155"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HPB00064.1"
                     /translation="MVFLILIFSAAIFVFFFEKQNNPDEYHNLWDAVWWVFVTIFTVG
                     YGDIKPITPGGRLVAIFIMLVGVSMVSTITATISSIFVTRKIREAQGLEAINYENHLI
                     ICGWNNRAESLINTILQLSGKKDMKLVLINELAENEINTILEKYSQFKIKFVYGDYSH
                     PAPLERANIKRARTVLLLPNLHRYSPADADEKTILATLNIKSSFPKVRVIAFIMNPEN
                     EAHVKRAKADEIVVSDKYADYFLAINILQPELPKVYDQILNPRMQNKIVVEAIPASFM
                     NKPYRELFDYYHTQYKTLLLGLISEVESIGFSDFLSADTSQLDAFIERKLRESGKSFG
                     EENKVFVKLNPGDDYLIQKNEKAIVLR"
     gene            complement(22193..23743)
                     /gene="gpmI"
                     /locus_tag="PLI55_04160"
     CDS             complement(22193..23743)
                     /gene="gpmI"
                     /locus_tag="PLI55_04160"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPB00065.1"
                     /translation="MRPVAIIIRDGWGYNENPRGNAVLAAKTPNIDSYKKNYPWTLLK
                     CSGEAVGLPEGYQGSSEVGHLNMGAGRIVIQELKRIDDGLSDGSLFESPKWKNLVANW
                     KANNSAFHLFGLLQDEGVHAHQEHLFKIMRRARQEFPEGEIIVHPFLDGRDTPPRSSL
                     EYIAKLNRVLAEVGHAKIGTIMGRYYGMDRSKNWNLTDTAYHCIVLAEGRPAPTAEAA
                     IRESYANDKTPDNVEMFDEYIPPYVIGGYTGMRDGDVILHTNYRQDRAIQLTRAFVDP
                     DYPGKLKAKPKVTYVGFTQYYDEFTEYLLGSMSSGGGMKNLLGEVISNAGLRQLRIAE
                     TQKFRHVTSFFNGKSTTPYPLEDQVEIKGRFDPATFASHPEMEAYNVTDELLRRLENN
                     PYTLIVVNYANGDMVGHTGVFEAAKKAIEIVDDNVGKIVRRLLELDAHILITADHGNS
                     EQMIDYETGMVKTSHTLFPVELIYVARDLAGKSLKSGGKLADIAPTALKLLGLEIPKD
                     MTADCLIS"
BASE COUNT         6714 a         5139 c         5000 g         6605 t
ORIGIN      
        1 ccctacatag ataatttccg aaatgataat tattttcaga tttctattct ttcaacgttt
       61 tccaacccac tgcattcaaa ttatggatta taatccagac actgatccca gccactaaaa
      121 aatacactcc tgctaaaaga tattcgtgaa aaatgatttt tcctattcca aacaggctgg
      181 catagaccag caaaacccca aaaatccagc agataaatgc tcggccgaaa ccactatcgc
      241 ccttgacatc aggtaattgt tcagcaattt tcttccaacc gctaccacca ggatgcacct
      301 tacgataaaa gctggtaaga gtagagtcct tttccggttt ggttaaaaaa gtcaccagta
      361 gccaaacaat cgtagtccct aggactaccg gataaagcgt tatcggggac tgtagtccga
      421 atttataccg tgcaattgga tagataatta gtggtgcaat catagccgaa atttccgacc
      481 aggcattaat acgccaccaa aaccagcgta aaatgagaac tgcacccatc cccgcactag
      541 cgttgatgat aaattcccat gctcctgata tagtagtcag gaaatatttc gtgacgaata
      601 atgaaaatac aatcataatc agcattgcaa ttcgggagac caatacataa tgtttttcac
      661 tagccttttt acgcaggaat cttcggtaaa aatcatttat aatatatgat gtgccccagt
      721 ttaattggga agagatagtt gacatataag cggctaaaaa agtcgcaatc aatagaccta
      781 aaattcctgt aggtaagtaa cgtaacatca tcttaggata cataactccg ggatcgacgg
      841 tattttcata tttttcgtaa aaattttgaa attccggggt agaagcaagc ggattagatt
      901 tcataagatc aggattttta tacgcttcaa cgacctgatg gtaaagtacc ggattttctt
      961 gctgaagtat atcggtactt gaggctcgcg gtagaatcac caatgccgaa agagctacaa
     1021 taatccaagg ccatggacgt aaagtataat gcgctattgt gaaccagaga gttgccaaaa
     1081 ggctgtgttt ttcattctta gcggacatca tgcgttgcgc aatgtaaccg ccgccgcctg
     1141 gttcagcccc tggataccag gacgaccacc attgcagtcc gatatgggca ataaaggcac
     1201 ccaatgatag agatagtacc ccaccggtaa ttccctctcc gttaatccgc cccactttgg
     1261 gaaagaaatc aagaacctgc ggtgccaatt gagctttcat tttaacggct ccaccaacct
     1321 ctggcagtcg gatgactata acagccaata taatgcaacc agtcatagca aatacaaact
     1381 ggaaactgtc ggtacgagcc gtcccaagca ggccggaagc tgaagcgtaa attgcgacaa
     1441 taccagcgac aattgctact aaaagaaacg ggtcaatccc tgggatcgtg acatggaata
     1501 ttttctccat ggctttatgt acccatccca taacaattgt attcataaaa attcccagat
     1561 atagggctcg gaatccgcgc agagccgccg ccgttttacc ggaataccgc atctcagtaa
     1621 attctacatc agtcataatt cctgctcggc gccatagtcg agcaaagaaa aagaccgtca
     1681 gcatcgcacc gatagccaga ttccaccata gccagttacc ggcgatacca ttcttagcga
     1741 cgagctccgt aacagctaaa ggtgtatcag ccgcaaaggt tgtcgctacc atggcagttc
     1801 cggctaacca ccaaggtaaa ttccttccgg agagaaaaaa ttcaccagtg ttttccccag
     1861 cccgccggta ataataaatt gcaatgccaa aaattagcgc aaaatatatt gccactacgg
     1921 cccaatctaa caatccgatt ttctgtgtca cttccatttt tacctctcat taaccttatt
     1981 aataactaat aattaagtag ttaattcgat attactaaat tctttgaagg cttatcaatc
     2041 gaaggaaaca tcattggtac aaatcgaatt tgcttaaatc aaaaatgttt aggtctaaat
     2101 attgactagg cttactgtac catttctgaa taattaccaa atatttaaat gtcaatcgcg
     2161 aaattcaacc ggattttcaa aataatatta tgaatctttt aaaatctgat ttccggcaac
     2221 agtgtgttca ggcatcaatt tttgcagagc ttcatgagcg gctttctgtt cggcattgcg
     2281 tttctgacga ccaacgccat atcccatttt ttgaccattg atatagaccg caacttcata
     2341 tttcttggca tgatccggcc cagagactct tagtacttta aaaaccggta agttgcgtcc
     2401 atttttctgg caatattcga aaagccgtcc cttgtaatta aaattactga cgactttgcc
     2461 ttcggctgtt gatggtataa tccagcgttg aacgaacttg cgggctgggc gcagtccacg
     2521 gtcgagatag attgcaccga ttaaagattc gacaatactg gcaagtaatt tatgcagagt
     2581 cggcggattt ttgagattta aacccttttc gacgatgact gctttttcga ttcccaattt
     2641 actaccaacg cgtgccaggg catttccatt tacgagagct gagcgatgca tcgtcagttc
     2701 gccctcactt ttatccgggt atttttcaaa taaaatctcg gcgacgataa aagccagaac
     2761 ggcatcgccg agaaattcta atcgctcata ggatttttcg ccattcaccg aacgcgagcg
     2821 gtgagtaaga gcctgttcca acaaagcttt attgcggaaa acgtatccta tttccttctc
     2881 aattggcgaa atcttgcgaa accaattttt taccgatact ttcaattttc cggcttgtaa
     2941 actttggcgg ccaggacggc attgtgcccc ccaaaaccaa aattgttact taatccagct
     3001 actagggttg ctttctgagc aatgttgggc gtatagttta aatcgcactc cgggtcggga
     3061 aattcataat taatagtggg cggtataatc tggttctgga gtgcaagcgc cagagtgatg
     3121 aattcgattc cgccagccgc acctaaaaga tgcccggtca ttgatttggt agaactgata
     3181 tgaatctggt aagcctgctt gccgaaaaca gctttgatgg cgatcgtttc gttacgatca
     3241 tttaacgggg tggaggtacc gtgggcattg atgtagccga atgctgattt cggcaaacct
     3301 gacattcgga tggcgttttc catggaaata atcgctccct tgccttctgg atgaggagcg
     3361 gtgatatgat aggcatcggc tgagaaaccg aagccgacga tttcggcata aattttagcc
     3421 ttccggttca gggcatgttc caattcttcg aggactacga tgccggatcc ttcacctact
     3481 acgaagccat cacgtttgct atcgaatggc cggctggctt tttgtggtgc attattacat
     3541 cgtgagaggg ctttcatatt ggcgaaacca gcgattgcca gcggtgttat agcggcttct
     3601 gcaccaccgg ttatcattac gtcagcagtt ccattacaga tgtgttgata ggcgactcca
     3661 atagcatgtg aaccagtggc acaagcagaa gtcacaccat agttagggcc gcagaaacca
     3721 aagtccatcg caatttggcc cggaattata tccggaatca tcatcgggat aaagagtgga
     3781 cttaccatgc gatgaccctt agtcatcaga atttgatgct gggcttccag ggtacttatt
     3841 ccaccaatac ctgaaccgac gattactcct actcgttcgg ctgagaaacc agattcaatt
     3901 aatcctgagt catatacagc ctgtttggcg gccgccgatc cgaattgagt aacgcgatcc
     3961 attcgattga tatccttttg agtgaagtac ttcagaggat caaaatcgcg tacctcggcg
     4021 gcaatctgag tggaatagcc gctggcatca aaagacttaa tgtaatcgac gccgctgacg
     4081 ccatttaata aattcgacca gaaagatggg acgtcacttc caaccgggga aatgacgccc
     4141 attccggtca caactacacg tttgggcata gacggggtta ttttaatttg gagtcgagat
     4201 aattgacaac atcaccgact gtttttagtt tttcagcatc ttcgtcagga atttcaatgc
     4261 cgaaatcctc ttccatcttc ataatcagct ccaccgtgtc cagtgaatcg gctccaagat
     4321 cgtcaatgaa agaagcttcc atcgtaattt tggatttttc gatccctaat ttttcagcca
     4381 cgatttcttg aactttttcg aagttactca tcatatcctc ctaagttagt ttacattacc
     4441 ataccgccgt caactttaag cacctgtccg gtgatataat cagccagcgg cgaagctaaa
     4501 aatagcgccg cattggccac ttcttgcggc gtccccattc tattcaaagg aatattcctt
     4561 aaaaattgcg ttcgaacttc atccgacaaa gcggctgtca tagccgtgtc aatgaatcca
     4621 ggcgcgatga cattgaccgt tatattccga gaagccattt ctttagccag cgatttggaa
     4681 aagccgatca caccggcttt actggccgca taattagatt gaccgatgtt gccagtcaag
     4741 cccactaccg agctaatgtt gatgattttg cccgactgct gttttagaaa atactttgcg
     4801 gccgctcggc aaaccagaaa aacgcctttt aaattggtat tgataactgc gtcccattgc
     4861 tcttccttca ttcgcaaaag gatggcgtcg cgggttaccc cagcgttatt gattaaaacg
     4921 tcaatgcgag tatatttttg aataatgctt tcaattaaat tttgtacttc agtagagttg
     4981 gttacatcac aaggaaggac atcaaaagga aaattttttg ccttaaaaag agcaacagca
     5041 tcagccagtg attcctgatt gtaatcgctt aaaatcacgg tggcgccatt ctcggcaaaa
     5101 gtctgagcga tggtcaaacc tatccctctc cccgaaccag tgacaatcac tattttatct
     5161 ttgaaagtta attccatgcg atctgctcca attcgctgaa tttagaaacg cctgttacat
     5221 taacatcagc cgaggtccgt ttgactaatc cctgtagaac tttcccgggt ccaacctcca
     5281 cgaagatttc cgctccgtca gcaatcatat tcctgatcgt gtcagtccag agcactgggg
     5341 aagtcaattg ctggtagagt cggttacgaa tttcctgcgg atcgcgcgta gccgtagcgg
     5401 tgacattaga ataaattgga caatccggcg ttttgaactc ggttttagac agagcttcct
     5461 tcagggcttc actggctggt tgcattaaat cggaatgaaa ggctccgccg actgataact
     5521 ccacagccat tttagcaccg gcttttttta aatcagtcat taaagcctgt actttgtaaa
     5581 catcgccgct gattactatt tggttaggag aattacaatt agcaatctga caaatgccag
     5641 aaaaattaga ttcactgatt atcttcttaa cctggtcata atccagacca acaatagcgg
     5701 ccattgttcc gggtttttgc tccgaggcaa tctgcatctg ttcagcccgt atttttacca
     5761 gatcaagccc atcctcgaag gtcagagcgc cgcaggcaac cagggctgag tattcgccga
     5821 gactatgtcc ggcaaccata tcagggaggt agcctcgcgc tttcaattcg aaaaatatga
     5881 tacaagataa cacgaaaatc gccggttggg taatgcgggt tttggtcaat tcactcagcg
     5941 ggccttcgaa ggaaaatttt tttaacggaa aaccgagccg agtttcagct aggtcataat
     6001 actggcgcgc cagagcactg ctttcataaa aatcgcgacc catcccgaca aattgcgaag
     6061 cctgaccggg aaatataaag gcaactttag tcattcggaa tgccccagcg taaaagaaga
     6121 ctgccccagg taaaaccccc accgaaagcc gccatcaaaa ccaagtctcc gcgcttgatc
     6181 cgtttctgat cgaatccctc aaccagcgcc agtgggatag ttgccgccgt ggtattggca
     6241 taacgatcaa tattgatcat tacctgttca tctgataatt ttaaacgatc agccgaagca
     6301 tctataattc gtttattggc ttgatgggga atgaataatt tgatgtcttt accagtcaga
     6361 ccattgcgtt ccagtacctc aagagagata tcagccatac ttttaactgc gaatttgaat
     6421 acagttttac cttcttggta aatatagtgc attttcttag ctacggtttc ggctgtggct
     6481 ggatgcaaac taccaccacc cagcatatac aaataatctt tgcccgatcc atccattttt
     6541 aagataaaat caataatgcc tgagtcgtcc ccgggaggag ttggctcgag caaaaccaca
     6601 cccgccgcgt ctccgaaaag cacacaggta tcgcgattgg tataatcggt aatgctactc
     6661 attgtatcag caccaaacac caatactcgg cggcaagtac cattttccac gaattgggcg
     6721 gcattagcta gagcataaat gaatcccgaa caagccgccg agagatcgaa tccataggca
     6781 tttttagccc cgatgccttc ttgaatcaag gcggcagttg atggaaagaa catatcggga
     6841 gaaatggtag ccacaataat caaatcaatc gtcagaggat cgacattaaa gcgtcgttga
     6901 agatcctgaa aggctttgat agccatagcc gaagtcgcct caccttcggc agcaatgtga
     6961 cgttcgcgaa tcccggtccg agtacggatc cattcatcgg tcgtgtcaac cattttctcc
     7021 aggtcagcgt tggttaaaat acgttcgggt acatacatcc caaacgcagt aatagtagat
     7081 ttaggttttt tcattagaaa ttttccttaa cctctattct tgtctctgac ccggttaaat
     7141 cgcaggatat gccataccgt cggaccgctt gtgaaaattt caaccagcta aagccctatt
     7201 tctctttagg tataattact ggccgcccac ggtaatagcc acaattgggg caggctcggt
     7261 gcggtaattt aggttcttta cattgagggc acttaacgac cgtggcttcg gtcaggactt
     7321 gatgagtccg gcgtttatct cttcttgatt ttgatgctct acgtttcggt tgtggcaatt
     7381 tattggtcct ccgtaatttt tatggttagt ttttttaatg aatcccagcg ggcatcaggc
     7441 tcagcgacat tgcaatgaca ttcagtcttg ttatgattag caccgcatat cgggcaaagt
     7501 ccctgacaat cttcttcgca gaggattttc atcggtatac tcaataaaag agtgtcgtga
     7561 atatgggtag ttatgtcaat ttcatttgtc attgatgaaa gttggataac attttcatcg
     7621 tctattttca cattagattc ggaataggta gcaatgattg cgaaagttcc acttattggc
     7681 atcgaaaaac tttccaaaca acgatcgcag tcaaatcggg cgatggattc aactttacca
     7741 tctattttta tttctgtggt gcccctgtca atgttcagca ttaccgccac cggggcgctt
     7801 agatcaaggt cagcgacatc cagctcatcg cccccgagag ctactctaat catctgcttt
     7861 ccatatttga gcgaattaat acgaactttc ataataactt gcaaatttat tggaaacgaa
     7921 cacgataatc aagaatttta acctctttcc gaggagaaac cacctattga actttgatta
     7981 tttccccata tttcaataaa attttccggc attgataatc cttgctttgt tccaggtgat
     8041 taaataaatt tagccggaac ttaattgaaa aataattgtt aaagtttacg atatttgaaa
     8101 aaggattaaa gaggaggcgt tcactgagtg ggacatcact cccatttcag gatgagtttc
     8161 atccaacttc cctccttcct tgggaactcc tcccgttaat aatagaacgc ctcctctcct
     8221 tttatcttca cggctaaatt ttcatagaaa tactttaaaa aggttgacgt atttttatca
     8281 aaaatcgtca ttttttggta aaattataaa aaggattttt gggtataaaa atatggattt
     8341 cgaagaaaaa tgtatcaata gtaaacgtgt ttttaatggg cgcttattac aggtttatcg
     8401 cgatgaagtt cgcctgccta atggtaaaat ttccaaccgc gaatggatca agcatccggg
     8461 agccgcagta gtcattcctt atctgggaaa cggccagatt cttctaataa agcaatttcg
     8521 ctatcccgtc aggcaaataa tgctggaact accagctgga aaaattgatc cggacgaaaa
     8581 cccggaacag accatccggc gtgaattagc cgaagaaacc ggatattcag cgcaaaaact
     8641 tactaaactt agcctaattc atacctgcgt tggctacagc aatgaatgtc tgcacttatt
     8701 ttgggcagac gaactccaaa aatgtgacac acgcccggat gatgacgaat taatcgaaat
     8761 tattcctatg tcaatcaatg cggctatgaa tctcctttac cagggcaaaa ttaccgatgc
     8821 caaaactatt atcggtttat tctgggctga taatataatt aatcgccgct atcttaatct
     8881 atgttctgac taaaccgtaa aatattaaat tttccccaaa ctaaatcact tatgggaaaa
     8941 ctgtaattct ttcctacatt gactttcagc catttcgact ataaattaat ttccaatgag
     9001 gctgaatcca gctactttat ttttgacttc cttgagacgt atttattttt tgattggttt
     9061 tgaagcaaat ggtcaaacct tgaggacaat tcaagaatgg cttaatccat ccggcttgca
     9121 taatatttcc tatgaccaac gatgatgttt atttccacta tttgatagca ataaagtcat
     9181 tttattttta attagaaaaa tgaagaggct aatatgaatt ttatctggtt aggtttgatg
     9241 ttgattgctc tgattgtcgg cgccttaaat ggacgcatcg agctggttat gaaggcggca
     9301 ttcgataatg cggccgcggc ggttgaaatt gccctgggct taatcggaat tatggcgctc
     9361 tggcttggca ttatgaaaat tgccgaaaaa ggcggtataa taaaaatcct ttcacgggca
     9421 atccgtccaa tagccaaatt tctattccct tccattcctt ccgatcatcc ggctatcggt
     9481 tcaatgttga tgaatatgat agccaactgg ttgggattag gcaatgcggc caccccactg
     9541 ggcttgaaag ctatggaaga attacagtct ctaaaccaat tcaaagatac cgcaacgaat
     9601 gatatggtaa ccttcttggc gctgaatacc ggtacaatct gtctgattcc aatgactgtt
     9661 attgcggtgc gtgctcaact cggttcagct aatccattcg aaataattgg caccacaatt
     9721 atttcatcaa cttgcgccac gattgcgggt gttacagccg ccaagctttt ccagcgattg
     9781 ccatctattc gcaaatccga tcccaatcgg ctgataaaac ctaattcaga tggggaagga
     9841 gtaaaacatg cctgatttaa actcgcctgc ggtagctgat acactgcaaa cctttgtcca
     9901 gaacagctct gaggtgtcac ccggactgtt tgttaggata atgcaaattt tttctaactg
     9961 gcttattccg gttctgattt ttgccattct gatactggct tggcgtaaaa aagtaaaggt
    10021 ttatgaggct tttattgatg gcgccaaaga aggcttctca gtcgctatca aaattattcc
    10081 ctatcttgta gctgttttgg tagcaatcgg aatgtttcga gcttcgggtg caatggatat
    10141 attcgttgct ttattttcac cgataactaa cctcattggc atgccagctg agactctgcc
    10201 ggtggctttg atgcgacctt tgtccggaag cggcgcgctg ggattggtta cagaattaat
    10261 gaaacagcat ggaccggatt cattcatcgg acgcgtcgcc tcgactatgt ggggcagtac
    10321 tgaaacgact ttctatgtag tagcggttta tttcggttca gttggagtgc ggcgggtccg
    10381 tcactcgatt gcggcgggac tggtcggcga tgcggtggga ttgataatgg cagttattat
    10441 ttgcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcttag gaggactgaa
    10561 tgaatccgta tattttccgc gaatatgata ttcggggcgt cgtagctgac gatttcagtg
    10621 atgaagtagt tgaattgctg ggtaagggca tcggcactta ctttcgggaa aaagatgcca
    10681 aaacaatttc gcttagcggc gatatccggc tttcgacgcc gcatttaaaa tctgttttga
    10741 ccaaagggct tttatcaact ggtctaaata ttattgatct gggtatcgtg ccgacaccgg
    10801 tcaattatta cagtatgtac gtcttgccgg tagatggtgc tatccaaatt accggcagtc
    10861 ataatccacc tgatatgaat ggctttaaga taagtctgga taaaaaggcc gtattcgggg
    10921 atcaaattca acatattaaa aatatcattg agaaaggcgt ttttgccggt ggcactggaa
    10981 aagtgagcga aaaatctttg ctggaagaat acaaacaaat gatcatatcc aaaatccaat
    11041 tagctcgtcc tttgaaaatt gctatggatt gcggtaacgg cgccgcatct ctgactgctc
    11101 cggaaatctt taaaaaaatt gggtgtcagg ttacagaact ctactgtgag gttgatggta
    11161 gattcccaaa tcatcatcct gatccgacgg ttcccaaaaa tttgaaagac ttaatcgcag
    11221 aagtacagaa aggcggctac gatttcgggg tggcattcga cggtgatgcc gaccgcatcg
    11281 gagtggttga tgatctcgga caaatcattt gggctgatta ccttatgatt ctgttccttg
    11341 aagaagttgc ccgaccgggt gtgccggtta ttttcgatgt caaatgttca caggcactgg
    11401 aagaaatgat aatatcttac ggggcaaaac cgattatgtg gaaaactggc cattctctta
    11461 ttaaacagaa aatgaaggac gaaaatgcac ctttcgctgg tgaaatgagc ggacatttat
    11521 ttatgggcga cgaatacttc ggctatgacg atgctgtcta tgtggcggcg cgacttgccc
    11581 gtttgatcgc ccgcggcaat gagaaaattt ccgctaaaac tgcgcgctta cctcgttatt
    11641 tcagtacacc ggaaattcgt ttggagtgta aggacgaccc cacaaaattc aagatcgccg
    11701 agcaggcggc cgcctatttc aagtctcatt acgactgcat agatgttgac ggcgtgcgaa
    11761 tcaaattcgg cgatggctgg ggattggtac gggcttccaa cactcagccg gtgattgtga
    11821 ctcgctttga ggcgaaaacc gccgagcgct tggatgaaat ccgtgatttg gtgcttaata
    11881 aattgcgcga attcggcgaa tttagacttt gataaagttt tacacgaata gggtctaatt
    11941 ttttttcctt gaaaattatt gtaaacttgg aagtgtattt tcggatgagg atttatgtca
    12001 gttgacttcg gcactaaaat cttacgactc aaagaagaaa tcgatcgcga actaagtaaa
    12061 attgatttat cgggaatctc gagtgaactc gtccaaccga tgcgctttag cctgaatgcc
    12121 aaagccaaac gcttgcgacc gattctggtg ttattagtcg gtgaaaattt aggtgccagc
    12181 cggaaaaatc tcatgcccgc cgctctggcg attgaaatta tgcatacttt caccctagtg
    12241 catgacgata ttatggacaa tgataccatc cgacgcggtc agcagactgt tcatgtgaaa
    12301 tggaatgtca atactgctat cctcaccggc gatgccttga tggcacttgc ttttcgcgtt
    12361 ctgatgcaaa ccaaatccga gcgtctcgat cagattggca ctcacttcag tcaagctatg
    12421 ctggaaattt gcgatggcca ggcattcgac ctggcttatg aaaaatatga tgagattgat
    12481 ctcactgatt atctgaatat gattgctcgc aaaaccggcc gacttctggg attagcctgt
    12541 cagctggcgg cgatcatcgc cggggcaagc ccgaaggtag taaataatct ctatcttttt
    12601 ggcttggaat tgggacaagc ctttcagatt caggacgatt tattggagct gacagctgac
    12661 caagctgaaa tgggtaaaag tctcgggagc gatctcaccg cagggaaaaa aacttttcct
    12721 attattatgg cgctgaatga gttgccagcc ggtcagcgcg ctgatttact taatttcctc
    12781 aaagccaatg ccggtaatcg gcgggctatc gttcaacaat tgaccaaacg cggttcaatt
    12841 caacagacgg aagctatgat cgcccggctt tttaaaaatg cccgtgaaag attgcacaat
    12901 atgcctgatc aattacaaag cgacttgata aatttaatag aattaatagc gggacgtcaa
    12961 tcctaagatg gtcagacaaa ccgttaaaat tctaaatcgg catggtctgc atactcgacc
    13021 ggctaccgtt ctggttaccc tttgttcccg tttcaaatcc gatatatttc taatctataa
    13081 aaatgtgcgc gtcaatgcta agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13201 nnntctgggc ttatgatgcc gcaatggagt ggtatgagaa cgctttgtta aatactatga
    13261 accaatttca gcaagagcgt ctgattgatc tccaggatat caaaaaacgt atgtttcgtc
    13321 atctttcgac ggaggaacaa cttgacctcc ctcagatacc cgaaagagta atatacgtcg
    13381 gtgagcggat ttcaccatct gaactaattc aattacataa tcagaaaata ctgggaatca
    13441 tcactcgtat cggtgggatg gattcacatt cgggaattct agcccgagca tttggaattc
    13501 cctacttatc caatgtcaat gagattgacc taatcggcaa aagtcaccaa gttattctgg
    13561 atgctgaccg tgaaatagtt attgtcgatg ccaaaccgtc cgtgcttaag aaatacgcag
    13621 taaaacttcg ggaattagaa attcgtcgtc agcgtctaaa tgaaaccata cccccagcca
    13681 tcacccgtga cggaatcaaa atcgaaattc tgctgaacgc cggattcctt tctgaagtta
    13741 aaaatatcaa cctgagtctg attgacggga taggtctatt ccgaacggaa tatctatgtc
    13801 ttgaacgcga cgatctccct aatgaggaag aacaatttag ggcttatcgc cagataacaa
    13861 tggctatgca gggcaaacca actacctttc gcactttcga ttttggtcgc gagaaaatga
    13921 tggctattct caatatggaa atgtttcaaa aagatgcggt ctttgacacc tggggtggaa
    13981 ttcgtttctg tttagacaac ccgcgaattt taatcaccca actgcgggct ttgctccgtg
    14041 ccagccagtt cggcccgctg aaaattatgt ttccgctggt ttccaattcc gatgaaatct
    14101 tacaggtact tgaaatctac cagcaagttc aatccgattt agaaaaagaa ggacatgcct
    14161 ttaatagaaa cattcctctc ggtgctatgg tcgaaactaa aagtattttg gacgaactgg
    14221 agaaactaac tgaattgctg aacttcttta gcgtcggcac caatgacctg gcactttttc
    14281 tactcggttc aaaacgagcc gataccatct ccaagaacta ctatcacccc aaaattttac
    14341 aagctatcaa tcaaataata aatgttactc aaaaaaagaa ctttccagtt acagtctgtg
    14401 gtgaaatggc ttccgatccc tacgctatcc tcggtttact ggctttggag gtacgctctt
    14461 tcagtatgag cgtcagcgcc ttgattgaca ttaatgactt aatccgaatg gtaaatattc
    14521 agaagatcag ttcactaaag gaaaatctgc tcaccgctga taacgttcaa caagtttata
    14581 aacttttacg aagctcctat aagcgtatta caaaaaataa gatgtaatag gggaaactta
    14641 ttttaatact tgactctgct tgacagttat gatatattca gacataagaa tatgatcaaa
    14701 gtcgcactca ataagatcat tttctacccg ccgagcaaag gctatgccct ccttttaaaa
    14761 gaagtcggtg gggaacgtca gctcccaatt attataggtg tttttgaagc ccagtccata
    14821 gcactcgcta tcgaggaagt gaaaatgccg cgtccaatga ctcatgattt attcgttaat
    14881 attttacacg atttaggcgc taaaatatta tcagtgactg tcagtgattt gatagatgga
    14941 actttttacg ccaagattga aatcagcctg gctaatagca agaaaaattt atatatagac
    15001 gctcgaccga gcgacgctat tgccttggca attcgcatga aagcacctat tcaagtaagt
    15061 gaggaagtaa tgcaggaagc tggacaatat ctgaactcca aacgtaaaga ttctcctgaa
    15121 aaagacgatt cccttccaga aatacagaat ctggatcatc tcttcgaatt gcagaataat
    15181 ctccaaagcg ccatcgaaga agaaaattac gaattagccg ctaagctacg cgatcagatc
    15241 aatcgcctta aacaagaatt aaacatcaat taaaaatcaa taaaagaatg tgatttacta
    15301 cctttcttta taataaggaa gcagttgcag catttattta ctcaaaaatc cgttaaaaaa
    15361 tattagaaaa aattccaaat tcccatttta ttggaaattc tgagaattat tttttaagca
    15421 attttaatct ggcaggcctt taacttgacg aagcaagtcg ccgccccttg aaattcgaca
    15481 taaatttacc taactttatt aagtgaaaat cattttgctc cctataactc acgatgtatc
    15541 tgccgccggc tcggtcgaaa gaacagatag ttataaaaat gccatnnnnn nnnnnnnnnn
    15601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15661 nnnnnnnnnn nnnnnnnnnn nnnnnaaata tccgtccgaa ttggctggaa aactttatcc
    15721 aaagggcatt ccaattttcc ctgaggagga actgacctac ctaattgaaa aattcgacgt
    15781 tgacgaatgc gtcttttcgt atagtgatgt gccctatcag aaagttatgg cactttcagc
    15841 gctcgtcaat gcggcgggag cgaatttcac cttactcgga ccaaaagcaa ctatgttaaa
    15901 aagcaccaaa ccggtgattt cagtctgtgc tactcgcacc ggttgtggaa aaagtcagac
    15961 agcacgcaag gtgattgaaa ttttaatggc gcagggtttg aaggtggtag ccgtgcgtca
    16021 tcctatgcct tacggtgatc tggttgctca aaaagtccag cgctttgcca ctttagacga
    16081 cttaaaaaag taccattgca ccatcgaaga aatggaagaa tatgaacccc acatcgtgcg
    16141 gggtaatgtc atctatgccg gagttgatta cgaggcgatt ttgcgagcgg cggaaaacga
    16201 tcccgacgga tgcgacctga tcatctggga cggcggcaat aacgattttc ctttttttaa
    16261 acccgacctg gcaatcacgg tcgttgatcc tcaccgaccc ggtaatgaac ttagttatta
    16321 tcccggtgaa gtcaacttac gcatcgctga cgttatcatc atcaataaaa ttgataccgc
    16381 taatccggaa gggattcaaa ctgtgagcca aaatatagcc atagttaatc ctcaggcaac
    16441 ggttatttat gcggcttcgc ccgttagagt tgaccatccg gaaattatcc gcgataaacg
    16501 agtgttagta atcgaagacg gtccgacgtt aacccatggg gaaatgcaaa tcggcgctgg
    16561 cataatagcg gctcgtcaat acggcgcccg ggaatgtgtt gatccccgtc cgttcgttgt
    16621 tggaaaactg gctgagacat tccaaaaata tccgaaaatc ggtcctcttt taccggctat
    16681 gggttatagt gaagaccaaa ttcgcgatct ggaagatacg attgcgcgga ccgattgcga
    16741 ttcagtcgtt attggaacgc cgattgatct gactcgcatt cttaaaatca aacagccatc
    16801 tactcgggtt tactacgaat tacaggaaat cggtactccc aatttaaacg aagtattatc
    16861 cgatttctgt caaagaatta aacttaatat tcaaacaaag cagatatgaa tcggaccgcg
    16921 gttgtagcat taggcggcaa tgccatctca cctaataatg agaaagacac aattgctaac
    16981 cagtttaaaa acacgcgcaa aagtctgcaa tcggtgatga atctaattcg ccagaattat
    17041 catctggcat tgactcacgg caatgggcca caggtcggca atgcccttct gcgggtcgaa
    17101 ctaacgattg acaaagcccc attcttaccg ctgggaattt gcgtagcgga taccgaaggc
    17161 ggtatgggct atatgatcga acaatccctc cagaatgccc ttataaaggc gcagataccc
    17221 cgggaggtgg tttcaattat tacccaggtg atcgtcgcag cagatgatcc ttcaattaat
    17281 aacccgacta aattcgtcgg aagttggtat tcctataatg aaatcaaaga actggcggaa
    17341 cgcttcggct ggcaaattaa agaggacggg caacgcggat ggcgacgagt agtgccttcg
    17401 ccaatccccc gcaaaattat taacagtcgt gctattaaac atttagttga tttaggcgcc
    17461 atcgtgattg cggcaggagg aggtggcatt ccagtttata taatggaaaa tggattatac
    17521 gaaggttttg atgccgttat agataaagac ctggcggcgg ctgtcttggc tcacgatatc
    17581 gcggctcagg agctgtatat tctgaccgat attgatagtg tcaaaattaa ttttggcaaa
    17641 cccaatgaag aatcaattga tttgatccat atccccgaat tgcaatgctt gctggcagag
    17701 ggccaatttc cattaggctc gatgggacct aaaatcagcg ccgctatcgc ctttatccaa
    17761 tctggcggtg aaagagtaat catcacctcc ctcgaaaaag caattgaaag cctgcatggt
    17821 gatagcggta caaccatcat cgcatagaaa ggaaaaatat gtcaatgaca cttgctattc
    17881 ttaaaccgga ttgtgttaaa cgtaaattga tcggcgaggt gattagtttc atcgaaaaag
    17941 aaggctttag gattgtttgc ctgcggatga ttcatcttca accgcaagaa gcggctgaat
    18001 tctatagtat tcaccgcggg aaggactatt tcgaaggttt aatgaatttt atgacctccg
    18061 ggccatctgt tttaatggtt ctcgagcgcg aaaatgctgt ggaacatttt cgcaaagtta
    18121 ttggagcaac tgatccaaag aaagccgctg aaggaacctt acgtcgaaaa tatgccgaaa
    18181 atgtacgata taacgttgtc cacggctcag attctgaaga aaatgcaaag aaagaaattg
    18241 catttttctt ttctacgatg gaaattatca aatgattaac atgcggaggg ccagatttat
    18301 gaaaaaacta tctttgacct taacgattct actgatgctt ctgagcaatt gtagcaaaaa
    18361 accggtggtc gtaacaggtg tcgtcggcaa tcccatcaat ttagttccgg agatcaaaaa
    18421 gtcggaagac actactggat gcacttttaa atggggtttt attgccaaac cggctgagag
    18481 tattatggat gttctctcgt ttatcccgga tagtcgcagt ttttcagttt cttttatacc
    18541 ggatgttccc ggcgattatg aattgcagtt tctcagtcag gacagcaacg gccaagaaaa
    18601 attcaaacaa attataaagt gtagcatatt gcccgatact gctcaatccc ctagtacaga
    18661 caccgttgct ggtcagatgc caccgcgaga tttgtcagca cctccccctc aatacgctca
    18721 atcctatccg ggcggggaaa acgaaacgac ctattcagct atgacccaat cccaaccgat
    18781 caaaaccgga aaaaacattc agaaaattcc tgggaaatat acaatccagc tgggcgctta
    18841 taaaaactat gacaaggctg aggccgaatt aaaaaaatta agagcacata atctggatac
    18901 ttatatacaa aaggcctatt ttaaggaaac taacgaaact tggtatcgca tccgcaccgg
    18961 aacctttgat tcttacgctg aagccaaaaa agttttgaac cagcttaagg cgcgttttcc
    19021 caaagagaat ctctggatag attatgttcg tgaagatcaa taaacaattg taggaggtta
    19081 taaatgaaaa aactgaaatt tgatgtaagg gatattttct tgactcctcg tttggcactg
    19141 agtggcaaga aaatctgggt tcatatggtc ggattagctg ctggctgggt aatctacacc
    19201 atacttgcat atgtagcttt gctagtcaat ggccaaagcc tggcagaaat ctggaggatg
    19261 tatcactttt tcccaattct atgctgtacc acctgtcaat taccctggta tagttggtta
    19321 atttacggag tgggggtggt cgcctggata ataattttct tactggcatc tgtagctgta
    19381 gcacgaatta cttacaaaca attaaaaggt gatgagtttt tctcatcagg cgacagtttc
    19441 aagtatgtca aaaaacacgg tgtcgccgct gtaatgggtc cggtttctat tttaatgata
    19501 attgcttttt tcgtaattct ggcgataata gccgcctggc ttgccaaatg gcctgtcctt
    19561 gatattattt ttctggggat accgtatctg ctctattttg tcgttgcaac ttttgtcgta
    19621 tatactgcgg tggtctttgt tgtgtcgctg gttctggctc cggcgatcgt cggtacagct
    19681 gaagaagaca ctatggagac cgtttttcaa agttactcca cactttgggc acagccctgg
    19741 cgattggttt tatacgaagg gattgtcgga ggactaacgg tagtggctac ttttatttac
    19801 ggctgggctt taatccttgg ctatcgattt tttaacttcg tatttagcgc tagctggcta
    19861 atggatggta aaatgtcccg cataatcgaa caggcttcct cttacctgtt cggcatcaat
    19921 tctccattaa ctccctggat tagccaattt tttaatgttt atactccggc ttcgctaggt
    19981 atagtaaaac cgctttatct tggaacaacc gaaattataa ccgtggtttt gataacgatc
    20041 gccttgttta taattgccgg aacggtcgtt gcttatgccc tttctaacct cagtgttggg
    20101 caagccctta tttacattat actacgtaag aaaaaagatg atgaggatct catagaacgg
    20161 aaagatgaag acgagttaaa agaggaagaa aaatccgagg agcctgcgag tgaggaaacg
    20221 aaagctccgg aatctgaaac tccttcttca gacgaaaaga cagagtagca aagtcttttc
    20281 gttggttttg ataaaaaggg cgtccgaatt ctgacgccct ttttgtttgg tttatatagc
    20341 ctgatatcgg gatagatttt ctataaaagt ttcatcggaa ccccaaccga gcaaatagtg
    20401 gatactttgc caattttaaa aattttgtca agagaaaaaa tacattcact attcgattag
    20461 cagactgagc gccgtggtca gtttcagaac gttctgattg gcttgcttca agcgctcggt
    20521 caagacgcgg gcaatatttt gcattacaat attgccgatc gtcggatttg agttgcatat
    20581 ctggaagaaa tctactctgc taatgcgacc gaccaggcat ttagtgctgg ctttgacagt
    20641 ggctgtgcgt ttgtcgtcat cgctaaagag actcatctct ccgaaaaatg ggcgggcggt
    20701 gctggacaat tttatcaagc ttttttcacg agtatctatc ccggacatat cggttttcaa
    20761 agtcaaagct tgcgaaatct cgacagtacc atccagaagt agaagaagtg aatcgccgac
    20821 ctcgccttcc ttgatgatcg tatcgccgga ttcgtattcg agaacctcaa ttacctcgca
    20881 aaaatgctca atgttttttt cggaaagact attgaaaatc tcatattttg ataaaatgga
    20941 aatatttaca tattgggctt tagtcttggt attcatcgga gaacaatcgc cttttcattt
    21001 ttttggatta gataatcgtc tcctggattt agtttgacaa agaccttatt ttcttcgccg
    21061 aaactcttgc cggattcacg taatttacgc tcaatgaaag cgtcgagttg agaagtatcc
    21121 gccgaaagga aatcggaaaa tccgattgac tcgacttcag aaattaagcc aagcaagaga
    21181 gtcttatatt gagtatggta gtaatcgaat aattcccgat aaggtttatt cataaaactg
    21241 gctggaatag cttcgaccac aattttattc tgcatgcgcg gatttaaaat ctggtcgtaa
    21301 accttcggta attccggttg taaaatatta atcgccagaa aataatcagc gtatttatca
    21361 ctaactacga tctcgtcggc tttagcccgt ttgacatgcg cttcattttc gggattcata
    21421 atgaaagcaa tgactctgac cttcggaaac gacgacttta tattcaaggt tgccagaata
    21481 gttttttcat cggcatcggc tggcgaatag cgatggagat tgggtagaag caaaactgtc
    21541 ctggcgcgct tgatgttcgc ccgttcgagg ggtgccggat gggagtaatc tccatagaca
    21601 aatttgattt taaattggga atatttctcg aggatcgtgt taatttcatt ttcggccagt
    21661 tcattgatta atactaattt catatccttt ttgccggaca attgtaaaat agtattgatt
    21721 aatgattcgg cgcgattatt ccagccgcaa ataatgagat gattctcata attaatagct
    21781 tccaaacctt gtgcctccct aatttttcgt gttacaaaaa ttgaagagat cgtcgcggtg
    21841 atggtcgaaa ccatcgaaac gcctactagc attatgaaaa ttgcgacaag ccttcctcct
    21901 ggagtaattg gcttaatgtc gccgtaacca accgtgaaaa ttgttacaaa aacccaccaa
    21961 accgcgtccc agagattgtg atattcatca gggttatttt gtttttcgaa gaaaaatacg
    22021 aaaatcgccg ctgaaaaaat taagattaga aaaaccaaaa atacttttag ggttgatcgg
    22081 gtaacgacag atttgaaggt ttcagaaaat ttcacattat ctcagattaa caattctaac
    22141 tgccaaaatt tcgttcgcta tcctttagca tcagcaatct tgaactttgt agttagctaa
    22201 tcaaacaatc agccgtcata tctttcggaa tttccagacc gagtaatttc agagcagtag
    22261 gcgcaatgtc cgccaattta ccgccagact tgagtgattt tccggctaaa tcgcgggcaa
    22321 cgtaaatcaa ttcaaccgga aagagagtat ggctggtctt aaccataccg gtttcgtagt
    22381 ctatcatctg ttcggagtta ccgtgatcag cggtaattag aatatgcgcg tccagctcta
    22441 agagacgtcg gacaatttta ccgacattat catcaacaat ctcaatggct tttttagccg
    22501 cctcgaaaac tccagtatgt ccgaccatat caccattggc ataattaacg acaatcaaag
    22561 tatagggatt attttccaag cgtcgcagta attcgtcggt tacgttgtaa gcttccattt
    22621 cagggtggct ggcaaaagtt gccggatcga agcgaccttt gatttccacc tggtcttcca
    22681 gcggataggg tgtcgtggac ttcccgttga aaaagctggt gacgtgccga aatttttggg
    22741 tttcggcaat acgtaattga cgtaaaccgg cgttggaaat cacctctccc aagagatttt
    22801 tcattccgcc accggaactc attgacccga gcaggtactc ggtaaattca tcataatatt
    22861 gagtaaaacc gacataagtg acttttggct tggcttttaa tttaccggga tagtccggat
    22921 caacgaatgc tcgcgtaagc tgaatggcgc gatcttgccg gtaattagtg tgcagtataa
    22981 catcaccgtc tcgcattccg gtataaccgc cgataacata cggtggaata tattcatcga
    23041 acatttcgac attatcgggt gtcttgtcat tggcatagga ttccctgata gcggcttcgg
    23101 cagtcggagc cggtcgccct tccgcaagaa cgatgcagtg ataggcagtg tcggttaaat
    23161 tccaattttt ggaacggtcc ataccgtagt aacgtcccat aatcgtacca attttcgcat
    23221 gcccgacttc tgctaaaacg cgattaagct tggcaatata ttccagcgaa ctgcgcggcg
    23281 gtgtatcacg accatcaaga aaagggtgca cgataatttc gccttcagga aattcctgcc
    23341 gcgcccgacg cataattttg aaaagatgtt cctggtgagc gtgaacgcct tcatcctgaa
    23401 gaagcccaaa aagatgaaag gccgaattat tggctttcca gttggctacc agatttttcc
    23461 atttgggcga ttcaaataac gacccatcgg aaagtccgtc gtcaatgcgt ttaagttcct
    23521 gaattacaat ccgcccagcc cccatattca ggtgaccgac ttcgctactc ccttgataac
    23581 cctcgggcaa tccgactgct tcgcccgaac attttagcaa tgtccaggga taattttttt
    23641 tataggaatc aatattgggc gttttagccg ccaagacggc attgcctctc gggttttcat
    23701 tgtaacccca accatcacga ataataattg ctaccggtcg catttttacc tctgattt
//
LOCUS       DAOOMU010000028        23611 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2047_length_23611_cov_24.411233, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000028 DAOOMU010000000
VERSION     DAOOMU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 23611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23611
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2047_length_23611_cov_24.411233"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..651)
                     /locus_tag="PLI55_04165"
     CDS             complement(46..651)
                     /locus_tag="PLI55_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00066.1"
                     /translation="MIKPVNEGELGRKAIHYFSSLIPIIYQFFFTRNEAVVIIGILFI
                     GIVVAELMRMHLPALRRLYYRLFGWMIRPHEFHNHFTGATYVFMGAFLAIFLFPKEVA
                     VTSLLILTVGDPTACLVGLSIGRIKIYNNKTLEGALAFIFVSLIATVWISSIPFWIKL
                     VGAVVACCVEVFHRKIDDNVLIPLISGTTIFFLVELGNFLT"
     gene            complement(648..1313)
                     /gene="rpe"
                     /locus_tag="PLI55_04170"
     CDS             complement(648..1313)
                     /gene="rpe"
                     /locus_tag="PLI55_04170"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPB00067.1"
                     /translation="MNHNKPTPEVVPSLLSADFARLKEHIRLVEKAGARRLHLDIMDG
                     HFVPNITIGPLVVEAIRRVTNLHLETHLMIERPERFIQQFVEAGSNTVILHVESAQDL
                     HNDLRKIKEFGVKAGVVLNPPTSFEEVEPYLDEIDHLLVMTVHPGFGGQQMIPEALDK
                     VRRAQPWQARYGFLIEVDGGINQETITIAAAAGADLLVAGNAVFGSGRPGHAFRSLTW
                     QIK"
     gene            complement(1303..2037)
                     /locus_tag="PLI55_04175"
     CDS             complement(1303..2037)
                     /locus_tag="PLI55_04175"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPB00068.1"
                     /translation="MSTKIRNCLRFIAAFIAVGVVLVLVFDKILMPIYVRHGKEVAMI
                     DVLKLPADKAIQMLDANGFEVQVVDTIESGNLPIGIVIDQQPPPGRMVKKGRVVQLVV
                     TGGSRFVRMPNLVGRALKAAQIILENNKLVLDSVEYLYSSEIPEGVVSEQSILPGSMV
                     SINTKVTVVVSKGPPSRQLEVPMLIGLSLTEARASIRKAGFRVGVIRYVPTTDLNPYT
                     VIDQTPKPGGLYDNPINIDLQVTIEP"
     gene            complement(2059..3378)
                     /gene="rsmB"
                     /locus_tag="PLI55_04180"
     CDS             complement(2059..3378)
                     /gene="rsmB"
                     /locus_tag="PLI55_04180"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPB00069.1"
                     /translation="MNTRAAAVTILNTLPATGYNYESAIDAFLKINNCSSPDSDFIYL
                     LVKGTIQNQSLLDYLIEQTRNNSVKRLEKIARNLLRVGVLQIVVLKTPPHAAVNETVA
                     AAHQLQRSDLAALINGVLRHIPQESVWRERLGQLELAEALAIEFSHPRWLVEKWLNAF
                     GRENTIDLLAFNNKYQAIFFRHNPLRISWSKFEAKLQADNYRIEIYDTAPVVFFLVDR
                     PGDLLKSEYFECGYCSVQDYSQSLAVRLLDPQPGEKILDACAAPGGKATMIAQLTKNQ
                     ANILANDRTINKIESIKESACRLGIDSLNYSVNDAAAAKFPMFDKILLDAPCSGSGVL
                     ARRADLRWSRKPGDLIQAQALQLRLLENLAGFLRDKGVLVYSTCSIEPEENWETVQMF
                     LERHPHFSVDTAVKYVDQRYCDQKGAVMVLPFEHKLTGSFAVRLVKN"
     gene            complement(3375..4304)
                     /gene="fmt"
                     /locus_tag="PLI55_04185"
     CDS             complement(3375..4304)
                     /gene="fmt"
                     /locus_tag="PLI55_04185"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPB00070.1"
                     /translation="MKIIFMGTPEFAVPSLRIIADSRHEIKAVVTKPDMPCGRGCRLQ
                     EPPVKKVARQLNLPILQPDSLKSPDFLAEMAQFSAELYVVVAFRILPPELLVIPPRGA
                     INLHASLLPRYRGAAPINWALINGEKETGVTVFQIKPTVDTGNILLQERVAIQPTDTY
                     GTLAPRLAEIGARLILAAIDGLEAGTLKGTPQDDGLATPAPKIFPELGEIDWYKPASD
                     LKNLIHGLSPTPGAFTFFRGKRLKILRASVIDEKIPGEPGTIIQYNKNSLNVLTGSGV
                     LKLEELQFEGRKPLPAAEFLCGFGGRVGDKFAS"
     gene            complement(4311..4847)
                     /gene="def"
                     /locus_tag="PLI55_04190"
     CDS             complement(4311..4847)
                     /gene="def"
                     /locus_tag="PLI55_04190"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPB00071.1"
                     /translation="MAVLPIYIYGSPILHKKLAPIDDLPPDFDRILSDMWETMYDDEG
                     IGLSANQVGLDLRFFVADFSLREENLGREVIINPVIIKTEGEVIGEEGCLSLPNIREQ
                     IPRAEKIILRYENRERKIIEREFNDLVARVIQHETDHLDGLTMVDRISPLKRSFLRSK
                     LKRLATAAAEGKFAKDIE"
     gene            complement(4851..5180)
                     /gene="yajC"
                     /locus_tag="PLI55_04195"
     CDS             complement(4851..5180)
                     /gene="yajC"
                     /locus_tag="PLI55_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPB00072.1"
                     /translation="MNSLLYAGATGGGSGTQTANPILSLLPFLLIILLMYFLMIRPQA
                     KKQKEKAKMLQEIQPGDEIVTIGGIYGKVEGVKDKNILIVRVAKDVKINISRSAVASK
                     VEEKVVS"
     gene            complement(5209..6777)
                     /gene="nadB"
                     /locus_tag="PLI55_04200"
     CDS             complement(5209..6777)
                     /gene="nadB"
                     /locus_tag="PLI55_04200"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPB00073.1"
                     /translation="MKAAEPYRMTDVLVIGGGLAGCSAAYAAAQAGLRVVLLTINQDA
                     SVSSSYYAQGGIIYKNPADTLADYIADFQESGAQLVNERVVEAIYTNGPRLIEQILIG
                     ELGVPFDRTPQGDIALTREGVHSRPRIAFVKDYTGRAIQERFHKKIRQIENIEVLPNT
                     TCVDLITLAHHSLNPLHIYEPSTCAGAYVYFQDSGEIHPILAKQTILASGGLGRLYLH
                     TSNPYHARGDGYAMAYRAGARLMNMEYLQFHPTTLFSTEDERFLISEAVRGEGGILRN
                     KDGEDFMPRYHPLGSLAPRDVVARSVMQEISRTKAHFVYLDITHKSADWLKQRFPQIY
                     EKCLSVKIDMATEWIPVVPAAHFECGGVAVDLLGNTTIRRLKAVGEVACTGLHGANRL
                     ASSSLLECLVFGTLAGRDCAERIGSVEIITPQVAPWQKENEPLDPDLIHQDRLTIRHT
                     MWNYVGIVRNAKKLDRAGGILWRLQQDIEKFYAHSQLNDELIGLRNAATTTLLVLHAA
                     RLNKFSRGCHYRVD"
     gene            complement(6780..7865)
                     /gene="nadA"
                     /locus_tag="PLI55_04205"
     CDS             complement(6780..7865)
                     /gene="nadA"
                     /locus_tag="PLI55_04205"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPB00074.1"
                     /translation="MNLNVSYPPPDLARLSQEEIERRIGKIRAQFGPQLIILAHHYQC
                     DQIVAQADAVGDSFALSRRASETKAKFIVFCGVHFMAETADLLKSAAQKVFLPNVDAG
                     CPLAEMANIENVKTAWGKIQALTGDQLIPVTYINSRLELKAFCGKNGGIVCTSSNAQA
                     ALEWAWSKKPKVFFFPDQHLGRNVALKMGISPQEIVIYDPRRPIDDKFAWQIKQARLI
                     IWSGFCEVHQAFSRNDIERLRQAIPGIKIIVHPECPEEVALNADFVGSTEYIIKTVTA
                     GAPGSAWGVGTERNLVDRLARQLPDRQVILLQEKGAYCSTMALLTPIHLLYQLECLRD
                     GRLINQVVADENLRSDALLAVQRMMEL"
     gene            complement(7918..9165)
                     /gene="tgt"
                     /locus_tag="PLI55_04210"
     CDS             complement(7918..9165)
                     /gene="tgt"
                     /locus_tag="PLI55_04210"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPB00075.1"
                     /translation="MSNHGRKESKEKNKLPFDIIAQDTGTKARAGIFVTDHGTVETPV
                     FMPVGTSGVVKTLTPAELQECRVQIILGNTYHLYLRPGLDVIRAAGGLHRFNAWPGPI
                     LTDSGGYQVFSLAHLGKLTEDEIVFRSHIDGSEHRLSPAISMEIQRTLGADIVMAFDE
                     CTPYPCDREYARQALERTHRWERLSREHFLKLKPLYGHRQFQFGIVQGSVYPDLRAES
                     VKALTEVDFDGYAVGGLAVGEPKEQMFEILSLVAPQLPEKKPRYLMGVGKPEDIVRAI
                     GWGIDMFDCVLPSRNARNGTLYTWKGRIILKQSQYRMDFAPPDPDCNCYTCRNFSRAF
                     LRHLFMNGEMTGLRLNTLHNVHFFMELVSRARQAILSGDYQNWQTQFFRNYPEEADHS
                     LENEARRSERRRKHLEENDINSV"
     gene            complement(9181..10533)
                     /gene="radA"
                     /locus_tag="PLI55_04215"
     CDS             complement(9181..10533)
                     /gene="radA"
                     /locus_tag="PLI55_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPB00076.1"
                     /translation="MPVKVKNVFVCNECGSVQPKWLGKCPECGAWNSMVAEQIQKKPR
                     SSAVERTRRNLVPLNQASTTSTQRLVTGITEFDRVLGGGFVSGSVVLIGGEPGIGKST
                     LIMQVIARLPGDRLLYFSGEESEEQLALRARRLEINDDRILVACENNLENILDQIENL
                     KPTAVVIDSIQTVYSDQFENVPGSVTQVRECAGRLLRLSKELNITTVFVGHITKDGMI
                     AGPKVLEHLVDTVLYLEGNKNNFYRTLRAVKNRFGSTNEVGIFEMKERGLIAVDDPAG
                     FFLTERREYVAGSAVTVSMEGTRPFLIEAQALVTQSSYGTPQRSANGIDYRRFVMLVA
                     VLEKRAGLPLRTQDVFINLAGGFKIDETGIDLGVTVAVASSFKDVPLEPQAAFIGEVG
                     LVGEVRSVPFLENRVREALKFGFTTVYVPKSGIKILEKSLQSKVTGIESLNQLFGFIF
                     "
     gene            complement(10523..11431)
                     /locus_tag="PLI55_04220"
     CDS             complement(10523..11431)
                     /locus_tag="PLI55_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB00077.1"
                     /translation="MKYFKLILICFIWSTTWAVIKIGLDQTPLMVGLALRFSVATIAL
                     GIIIFLKRRKIAFDRVSVQNYLVVGLLTMALSFYCTYWAEKFIPSGLTSILWTAMPIV
                     VGIFAHFMLPDERLRLAQVMAILIAMAGVIVILSDRQLVFGRELLWGSVVALFGVVVS
                     SWPNVLLKTRPQPYDSLTMTAMAMLIAALVNFIGATVGGEWQKMVWDFKNIGAIVYLG
                     LFGSALAFYIYFDLLKQINVIKLTFINFISPIFATLIGALWLNEIVTWREIGGMLIIF
                     FGLFLYDKDKYLAILKIKNGRSTHAR"
     gene            complement(11519..>11751)
                     /locus_tag="PLI55_04225"
     CDS             complement(11519..>11751)
                     /locus_tag="PLI55_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPB00078.1"
                     /translation="QGVTFLDFGSTWYDNKFHPFKNDYYRGFMMDDLVAGYGMGARIY
                     LGVFVLKIDVAWRFDFDRTYSPRWYFCLDTDF"
     assembly_gap    11752..11851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11852..>13512)
                     /locus_tag="PLI55_04230"
     CDS             complement(<11852..>13512)
                     /locus_tag="PLI55_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00079.1"
                     /translation="TQLINDKYPDFEPSWSPDGQRIVFVSQRTPEIDSLPNLPEIMNV
                     KCFQTDLFCLDLDNGEITRLTDSPWNENYPCWAHTKDAIFYTSDYHGISNIFMLDIST
                     GESGAITNVLTGVFQPSISNDDLRLVLAGYADEGWDIYSISNPLQLWDERQEIKPTVY
                     AKALMESWENPEKYQRFNFPSKEEAKPRTRQNIIAAGDFSNYIFAPMYNYEISAEPEE
                     DFPDSTMASEDTTKQTTKSGRYPVFPYKTNFSLDVAYGEAGYSNLWGLQGTTVFYFSD
                     VLGNHQLALGTEMYIDLENSDYYLSYMYLAKRTNWGITGFHTSNFWNYGFFTVFRLRN
                     YGLDFSASRPSSHFSRTELGLTSYNVEQKYILTLTGEELYTYRINTILPRVGFVYDNS
                     LWGYLYPIDGWRARVDFIASPKYSAKSLEFYSVQADIRRYLKLNMDYSFGLRLSTGFS
                     NGKNAQRFFLGGEDNWINYYYRYDIFTADSTLAENIYFSDFITPLRGALYYERAGNRY
                     FLFNLEFRYPFIRQLVLGWPLPVTMGGIQGVTFLDFGSTWYDNKF"
     assembly_gap    13513..13612
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13613..14619)
                     /locus_tag="PLI55_04235"
     CDS             complement(<13613..14619)
                     /locus_tag="PLI55_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter Tol"
                     /protein_id="HPB00080.1"
                     /translation="MIKKLKSILLILIFATAGFSQYGKNKVQYDTFNWSYLQSENMDI
                     YFYEGGESLAEFAAPVGEQALKDICKVLNWRLRKRVTLIVYNSHSDFQQTNVTLSYLY
                     EGIGGFTELFKNRAVVPFEGSYFDLWHVIRHELVHVVINDMIYGGNVQSVVSGRVRLS
                     IPDWMSEGLAEYIAYGWTAQTDLIMRDIALNSNIPNIEELDYYSAYQGGNSVYRFIAE
                     KYGIEKIGQIWAQMKSKGNANNGLKAAIGMDMKELTDKWHRWMRTEYWPDVADRYDIE
                     EISMKLTDHKKLKNYFNTAPAISPLGDKIAIMTDRNGYADIYLISAVDGKVIKKLISG
                     QR"
     gene            complement(14775..15206)
                     /locus_tag="PLI55_04240"
     CDS             complement(14775..15206)
                     /locus_tag="PLI55_04240"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPB00081.1"
                     /translation="MVKKSVLFICTGNSVRSQMAEALLRQLGGDQFEVFSAGSTPVGI
                     HRFTKQVLAEIGISVDNQYSKHIDMYRDYNFDYVIVLCEVAALNCPTFPGQAERLEWL
                     IDDPIRVIGNEERRLMAFRITRNILKERIEKFIKEKGNPST"
     gene            15527..17047
                     /locus_tag="PLI55_04245"
     CDS             15527..17047
                     /locus_tag="PLI55_04245"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HPB00082.1"
                     /translation="MKRREFIKKSGVIAAGIVTAPAWFDMAGFSKTVSETQDLENLFN
                     ISAVEAEKLLRLALSRGAQSADLFFEYRPNSSLRIEDGIIKDIQYGISLGMGVRAVKG
                     DQIGYAYTDAIDFQSMKRAAETAANIANSTGSFKPARFQKVAAKNLYPVKSYSIDMDL
                     NPKITMLNEADATARQFNARVSSVIASFSEELRQIMYFDSNGKKFQDIQPLMSLRVQV
                     IAEDGVRRQGASASRSARAGLEFFEKPENTPSAVARQAAATAIINLDAKPAPAGPQVV
                     ILGAGESGVLIHEAVGHGLEADYSYKNLSNYSGKVGQKVAAENCTIIDSGLFPGKRGA
                     LNVDDEGNLPTTTVLIENGILKGYMHNSISARMMKSNPTGNGRRQSYAYPPLARMTTT
                     YLQKGNYEPEEIISSVKKGVYAKGFSGGQVDTIKGDFTFGVTEAYLIEDGKLTAPLKD
                     VTLIGNGPDTMNKILMVGNDLKISDGTWSCGKDGQNIPVSVGNPTVKISEITVGGS"
     gene            17062..18408
                     /locus_tag="PLI55_04250"
     CDS             17062..18408
                     /locus_tag="PLI55_04250"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HPB00083.1"
                     /translation="MQELVKIAENVISEARRGGADEVDVLVNNFTNTRLKIRLGKVEE
                     LRQSNPKALGIRVFKNKRKALTYTSDLRPESISKMVQKALEIVRVSSTDEYNGLPEKH
                     LFGKVQADLKLYDPEIAQIPTERKIKMATELEDLGLKQDPLITNSGGSFWNDSYGQSI
                     LINSQGFSGVQEFSSCSLSLQLVAEKDGVKQTDYWWSGQRAFNRLDSIESIAKTAAAR
                     TIRKIGARKPTTQSVPVVYDPQTGADLLAILASVVLGSAIYRKNSFLVDKLYQPIAIE
                     ELNIIDDATLPGGIDSRYFDDEGLPARRNIVIENGILKTYLCDCYSARKLKCTPTGSA
                     RRGTSTDPSPSTSNFFLPAGKYSPEEIIGSVSNGLYLTDLNWVGINYVTGDYSRGAEG
                     IWIENGKLAYPVQEFTVAGNVLEMLKSISMIGNDLEFRGSINSPTFKIERMMVSGQ"
     gene            18420..19172
                     /locus_tag="PLI55_04255"
     CDS             18420..19172
                     /locus_tag="PLI55_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPB00084.1"
                     /translation="MKIVVCVKVVPVSDSMITIAPEKTAIDLVNIKFDLNPYDEFAIE
                     AAVQLKEKNGGEVIAISLGPERDNISLKRVLALGADRMIHLVTADKALSDPLATAKAL
                     ATEIKSLSPDLIFCGKLSIDTGNGLVGPLVARLLGYPFLTGICELQVGTDQLTAAKDT
                     EEGRIIYESSLPCLLTTEKGLNKPRYPSLKDIMLSRKKEIITKNVTLTEKASEIIQLL
                     EPAPRPAGTILGQNVDAVPELIRRLREEAKVL"
     gene            19185..>19573
                     /locus_tag="PLI55_04260"
     CDS             19185..>19573
                     /locus_tag="PLI55_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00085.1"
                     /translation="MKIFTYIEPENGNISKNSAGCLTLGRQLADLANGSLTAIVPAQE
                     SSTMQNECGHYGADRVAFTKNFAKYIPEVTASDVANILLEENGDILILPDRPAIRDIA
                     AIACETVSGSIAVDCLQVNLDSENSPIV"
     assembly_gap    19574..19673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19674..20015
                     /locus_tag="PLI55_04265"
     CDS             <19674..20015
                     /locus_tag="PLI55_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPB00086.1"
                     /translation="GRGIGAPENFHLIEELADALGAAVGASRSVVDAGWVPQTFQVGQ
                     TGKTVSPDLYIACGISGAMQHIAGISSAKYIVAINNDPDAPIFKIANYGIVANLFAII
                     PEMIKHLKNIK"
     gene            20012..21112
                     /gene="plsX"
                     /locus_tag="PLI55_04270"
     CDS             20012..21112
                     /gene="plsX"
                     /locus_tag="PLI55_04270"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPB00087.1"
                     /translation="MNNLDNKQPSAVIAVDAMGGDFAPDEVVLGAARASVETPIKIIL
                     VGDQSKIEPILEQTEHNPQQLEIVHTDQYITNDDKPKFILSKKPRASMLIASQLCGAD
                     QAHGIVSAGNTGAYILSAIKYIPRIEGVHKTAIASAYPTRNEQGRSDPFALLLDVGAN
                     VHCTADDLVQFAIMGKIYASAVKGIENPTVGLLNIGKEPYKGGDTLTDAFNMLNSIQD
                     MKFIGNIEGNEILKGIADIIVAEGYVGNIVMKTIEGFGETISHIGRYAFKHRFIWMLG
                     LLALSSGIKQLKKVADYSEYGGAPLLGFRKIVIKAHGRSRAKAITNAIIVAAKTYHDN
                     VCEKMAREIAQYEKQFQRKNVMNCDWKLNFDQ"
     gene            21179..22723
                     /locus_tag="PLI55_04275"
     CDS             21179..22723
                     /locus_tag="PLI55_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPB00088.1"
                     /translation="MTKFSLVKSAAVLGIDAYIVDVECNLSNAQLPAFITVGLPEGAV
                     RESKERVWSAIKNLGYYVPSNKIVVNLAPADIKKEGSSFDLPIAIGILTAADLIEKHK
                     VSDYIFLGELSLDGMLREIKGALPISVSVRNSGIKGIILPEQNAREAAITGDLEIIGL
                     KSLQEVIDFLENRTHPEPTHVDVRALFEEHRQYQIDFSEVKGQENVKRALEVAAAGGH
                     NIIMIGPPGSGKTMLAKRIPTILPNLTLEEALETTKIHSVAGLVPSGQGIVATRPFRS
                     PHHTISDAALVGGGKFPRPGEVSLSHHGVLFLDELPEFKKNVLEVMRQPLENGYVTIS
                     RALLSLTYPASFMLVAAMNPCPCGYATDPHHQCTCTPQQIQKYMARISGPLLDRIDIH
                     IEVPAVKFSDLSAKAPGEPSEIIRERVQAARLKQTARFAGIKGVFANAHMESRLIRRF
                     CQIDQTCEDLLRNAINKLGLSARAYDRILKVSRTIADLSNSEKILPEHISEAIQYRSL
                     DRHLWL"
     gene            complement(22730..>23611)
                     /gene="dnaA"
                     /locus_tag="PLI55_04280"
     CDS             complement(22730..>23611)
                     /gene="dnaA"
                     /locus_tag="PLI55_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPB00089.1"
                     /translation="KTHLLQAIGNEALNNGRAKKVVYVSSDTFTLDFIAAIQNNKTTE
                     FSRSYRSIDMLLLDDIQFFQNKEQTQEQFFHIFNDLYQRHKQIVLTTDKPPMELRGLQ
                     ERLLSRFQSSLTVDIQPPDLETRIAILQKKANDDGLDIPYEVIEYLSANIRSNIRELE
                     GALIRLLAYSSLLQVDIDINLTQRVMREILGTRHSSVLSIESVQSTVSTVYKINLDSM
                     VGKGRTKEVAEARMIAMYLTREFTDLSLKTIGLYFGGRDHSTVVHACRWVEERLQSDS
                     VFARQINDLKAKLSQNI"
BASE COUNT         6677 a         5696 c         4831 g         6107 t
ORIGIN      
        1 ttacaatgta ttaaacaacc aaacatcttt atttttatga attattcagg ttagaaaatt
       61 acccaattca accaggaaaa aaatcgtcgt cccactgatg aggggaatca gtacattgtc
      121 atcaatcttg cgatgaaaaa cttccacaca acaagcgaca acagcgccaa ccaattttat
      181 ccagaagggg atcgagctaa tccataccgt cgctataagt gatacgaaaa taaatgctaa
      241 tgccccttca agggtcttgt tattataaat ttttatccgg ccaatcgaga gcccaaccag
      301 acaagcggtc ggatcgccaa ctgtcagaat taaaagactc gttacggcaa cctctttcgg
      361 gaatagaaat atcgccagga aagcgcccat aaatacatat gtcgctccag taaaatgatt
      421 atgaaattca tgtggtctga tcatccaccc aaacaaacga taatagagcc gacgcagtgc
      481 cggaaggtgc atacgcatca attccgcaac aactattcct ataaatagaa ttccgataat
      541 tactactgcc tcatttcttg tgaagaaaaa ttggtagatt attggtataa gggaagaaaa
      601 ataatgaatc gccttgcgac cgagttcccc ctcattgacc ggtttaatca ctttatttgc
      661 caggttaagg atcgaaaggc atgacccggg cgtccactac caaagacagc gttgcctgca
      721 actaataaat cggcaccagc cgccgcggcg atcgttattg tttcctggtt aatgccacca
      781 tccacttcga tcaaaaaacc atagcgcgcc tgccaaggct gggctcgccg tactttgtcg
      841 agcgcctcgg gaatcatctg ctgaccaccg aatcccggat gaaccgtcat aaccagcagg
      901 tgatcaattt catccaaata cggttcgact tcctcaaagc ttgtcggtgg attcagtact
      961 acaccggctt ttaccccaaa ttctttgatc ttacggagat cgttatgcaa gtcctgggct
     1021 gattcaacgt gtaaaattac cgtattacta ccggcttcaa cgaattgctg aataaagcgc
     1081 tctggtctct cgatcatcaa atgggtctcg agatgcagat tcgtcacacg cctaatggct
     1141 tccactacaa gaggtccaat ggtaatattg ggaacaaagt gcccatccat tatgtccagg
     1201 tgcagacgcc gcgctccagc tttctcaacc aggcgaatat gttctttcag tcgggcaaaa
     1261 tcagccgaca ggagagaggg aacgacttcc ggtgtcggct tgttatggtt caatggttac
     1321 ctgcaaatca atgttaatag gattgtcata taaaccgccc ggtttaggag tctgatcaat
     1381 cactgtatat ggattaagat cagtggtcgg tacatagcga ataacaccaa ctctgaaccc
     1441 ggctttccgg atactagctc gggcttcagt caggctgagc ccaattagca tcggcacttc
     1501 aagttggcgg cttggcgggc ctttactgac cacaactgtg actttagtgt taatgctaac
     1561 cattgaaccc ggcaaaatag attgttcgct gaccactcct tcaggaatct ctgaggaata
     1621 gagatattca accgaatcga gaacgagctt attattttcc aagatgattt gagcggcttt
     1681 cagagcacgg ccgaccaaat tgggcatacg cacaaaacgg ctaccaccag tgactactaa
     1741 ttgtacaact cgccctttct tgaccatccg tccaggaggc ggctgttgat caataacaat
     1801 ccctatggga aggttaccac tctcaatagt atctacgacc tggacttcaa aaccattggc
     1861 atctaacatc tggatagctt tatcggccgg tagcttgagc acatcaatca ttgcgacttc
     1921 ctttccatga cgaacataga taggcatcaa tattttatca aaaactagca ccagcaccac
     1981 gccgactgct ataaaagccg ctatgaatct caaacagttg cgaatctttg ttgacatttg
     2041 ctcctgacta cttctatttc aattttttac caaacggact gcaaagcttc cggtcagttt
     2101 atgttcaaat ggcaagacca taactgcccc cttttgatca cagtagcgtt gatcaacgta
     2161 ttttacagcc gtatccactg aaaaatgcgg atggcgctct agaaacattt gcaccgtttc
     2221 ccaattctct tctggctcaa tggaacaagt gctataaact aaaacgcctt tatcacgcag
     2281 aaatcctgct aaattttcga gaagccgtaa ttgtaaggct tgcgcctgaa tcaaatcccc
     2341 cggcttacga ctccagcgca agtcagcccg ccgcgccaga acgccgctac cactacacgg
     2401 tgcatctaat agaattttat caaacatagg aaatttcgct gcggcggcat cattcacact
     2461 gtaatttaag gaatcaatcc ctaatcggca ggcggattct ttgattgact caattttatt
     2521 aatcgttcga tcatttgcca ggatattggc ttggtttttc gtcaactgtg ctatcatagt
     2581 cgccttgccg ccgggtgccg cacatgcatc caatattttt tcgccaggtt gcggatccaa
     2641 aaggcgcacc gccagcgatt gactataatc ctgaaccgaa cagtaaccgc actcgaaata
     2701 ttcactttta agtaagtcac ccggtcggtc aactagaaaa aatactaccg gagcggtatc
     2761 gtatatctca atccggtagt tatcagcctg tagttttgcc tcaaatttgc tccaactaat
     2821 gcgcagtggg ttgtgccgaa aaaaaatcgc ttgatattta ttattaaaag caagcaaatc
     2881 aatcgtattc tcacgaccga aagcattcag ccatttctct actaaccagc gcggatggga
     2941 aaattcgatt gccagcgctt ccgccaattc taattgcccc agacgctcac gccagactga
     3001 ttcctggggt atgtgtcgta aaacaccatt aatcaaggcg gctaaatccg agcgctgaag
     3061 ctgatgggcg gccgctacgg tctcattcac cgccgcgtgg ggcggcgttt tcaaaaccac
     3121 aatctgcaga actccgactc gcaatagatt gcgagctatt ttctcaagac gtttgactga
     3181 attgttccgt gtttgctcga ttaaataatc cagcaagctt tgattttgga tagtgccttt
     3241 taccaacaga tagatgaaat cgctgtccgg tgagctgcag ttattaatct tgagaaaggc
     3301 gtcaatggct gactcgtagt tatagccggt ggctggcaaa gtattcaaaa tcgtaacagc
     3361 ggcggcacga gtattcacga ggcaaattta tctcccactc gtcctccaaa accgcataaa
     3421 aattcagcgg caggtaaagg tttgcgccct tcaaattgca actcttccag ttttaaaact
     3481 ccactaccgg taagtacgtt caggctgttt ttattatatt gaataatcgt tcccggttca
     3541 cccggaattt tttcatctat gacagaagcg cgcaaaattt tcaatctttt gccccggaaa
     3601 aaggtaaaag ctccgggagt aggcgataat ccgtgaatca ggtttttcaa atcgctagct
     3661 ggcttgtacc aatcaatttc tccaagttca gggaaaattt taggggcagg tgtagccaaa
     3721 ccatcatcct gaggtgtgcc ttttagagtt ccggcttcca gaccatctat agccgccagg
     3781 atcagacgcg cacctatttc cgctaagcgc ggcgccagcg ttccataagt atcagtaggc
     3841 tgaatcgcaa ctctttcctg caataagata ttaccggtat caacggtcgg ctttatttgg
     3901 aacaccgtca cgccggtttc tttttcaccg ttgatgagcg cccaattgat cggtgccgca
     3961 ccacgatagc gcggcaagag ggaagcatgt aaattaatcg cgccgcgtgg tggaattacc
     4021 agaagttcgg gtggcaaaat ccgaaaggca accactacat aaagttcagc cgagaattgt
     4081 gccatttcag ctaaaaaatc cggtgactta agcgagtccg gctgtaagat cggcaagttc
     4141 aattggcgtg caactttttt taccggcggt tcctgaaggc gacatccacg gccacaaggc
     4201 atatccggct tggtaactac cgccttaatc tcatgccgcg agtcggctat tattctcaag
     4261 gacggcacgg caaattccgg cgtgcccatg aaaatgattt tcatctgaac tcactcaata
     4321 tctttggcga acttgccttc agcggcggca gtcgcaaggc gcttcagctt tgacctcaaa
     4381 aaactgcgct taagcggact gatgcggtca accatagtta atccgtcaag atgatctgtc
     4441 tcgtgttgaa tgacgcgcgc taccaaatca ttaaattcgc gctcgataat cttgcgctct
     4501 cggttttcat agcggaggat gattttctca gcccgcggta tctgttctcg aatattaggc
     4561 agactcagac aaccttcctc gccaataacc tcgccttccg tcttgattat caccggatta
     4621 attatgacct cacgccctaa attctcttcc cgcaaagaaa aatccgctac gaaaaatcgt
     4681 agatcaagcc ccacctgatt agccgagagt ccgattcctt catcatcata catcgtctcc
     4741 cacatatcgg aaagaatgcg gtcgaaatct ggcggcaaat catcaatcgg agccaatttc
     4801 ttatgtagaa tcggactacc ataaatgtaa atcggaagta ccgccatctt ttacgaaacc
     4861 actttttcct ctaccttact ggcaactgcc gaacgagata tgttaatttt gacatcttta
     4921 gctactctca caatcaggat atttttatcc tttacgcctt cgactttgcc gtaaattccg
     4981 ccgatagtaa caatttcgtc acccggctga atttcctgca acattttggc tttttccttc
     5041 tgctttttag cctgcgggcg aatcatcaaa aaatacatta acaaaatgat cagcaaaaac
     5101 ggcaacaatg aaaggatggg attggctgtc tgggttccgc taccaccacc tgttgctcca
     5161 gcatataaca gactattcat ataacctcca tttttattac aattttaatt aatctacacg
     5221 ataatggcaa ccgcgactga atttattcag gcgagcggca tgtaaaacca gcaatgtcgt
     5281 cgtagcggca ttgcgcaagc cgatcaactc atcattcaat tgactatggg cgtaaaattt
     5341 ttcgatatct tgttgtaagc gccataaaat tccaccggct cggtctagct tcttggcatt
     5401 tcggacaata ccaacataat tccacatggt atgtctgata gtcagacgat cctggtgaat
     5461 aagatccggg tcgagcggct cgttttcttt ttgccaaggg gctacttgag gagtaatgat
     5521 ctccacactc ccgattcgtt cggcacaatc gcgtcccgcc agtgtcccaa aaactaaaca
     5581 ctccagaaga gagctgcttg ccagacgatt agcgccatgt aagccagtgc aagccacttc
     5641 accgacagct tttaaacgcc gaatagtagt gttgcccaga agatccaccg ccactccgcc
     5701 acattcaaag tgagcggcgg gcaccacggg aatccattcg gttgccatat caatcttcac
     5761 cgatagacat ttttcgtaaa tctgcggaaa acgctgcttc agccaatcgg cgctcttatg
     5821 agtaatgtcc agatagacaa aatgagcctt ggtgcgactg atttcctgca taacgcttcg
     5881 ggcaacgaca tcacgcggcg ctaatgaacc cagcggatgg tagcgcggca tgaaatcttc
     5941 gccatcttta ttccgtaaaa taccaccctc gccccgcaca gcctcgctaa ttaaaaagcg
     6001 ttcgtcttcc gttgaaaaca gcgtggtcgg atgaaattgg agatattcca tattcattag
     6061 acgtgcgcct gcccgatacg ccatagcata gccgtcaccg cgagcatgat agggattcga
     6121 tgtatgcaaa tacaaccgcc ccaatccacc cgaagccaat atggtttgct tggcaagaat
     6181 tggatgaatc tcaccactgt cttgaaaata tacataagcc ccggcgcagg tgcttggctc
     6241 ataaatatga agcggattta aagaatgatg cgccagggtt atcaaatcca cacaagtagt
     6301 attaggcaat acttcaatat tttcgatctg tctgattttc ttgtgaaaac gttcctggat
     6361 cgcccgaccg gtataatcct tgacaaaagc aattcttggc ctggaatgaa ctccctcgcg
     6421 agttagggca atatcaccct gcggcgttcg atcgaacggc actcccagtt caccaataag
     6481 aatttgctct atcagacgcg gtccattggt gtaaattgct tcgactactc tttcatttac
     6541 taattgcgct cccgattcct ggaaatctgc tatgtaatct gccagcgtgt cggctggatt
     6601 tttatagata ataccacctt gcgcataata agacgacgag acactggcat cctgattgat
     6661 agtcaataat acaactcgca atccggcttg agccgcggca taagccgccg aacaaccggc
     6721 taagcctcca ccgatcacca aaacatcagt catgcgataa ggttcagcgg ctttcaaagc
     6781 tacaactcca tcattctttg tacggcaaga agcgcatcgc ttctcaaatt ttcatctgct
     6841 accacttgat tgatcaggcg gccatctctc agacattcca attgataaag caagtggata
     6901 ggtgttaaca aagccattgt cgagcaatat gcaccttttt cttgcaataa aatcacctgg
     6961 cggtctggca actggcgcgc cagacgatca accaaatttc gctcggtacc aactccccag
     7021 gcactgcccg gcgcccctgc cgtaacagtc ttaataatat attcggtgct ccctacaaaa
     7081 tcagcattca acgctacctc ttccggacac tccggatgaa cgataatctt aatacccgga
     7141 atagcctggc gtagcctttc tatgtcattt cttgaaaaag cctgatgcac ctcgcaaaac
     7201 ccactccaga ttatcaggcg ggcttgttta atttgccatg caaatttgtc atcaattggt
     7261 cgtctcggat catatataac gatttcctgc ggcgagattc ccatttttaa agctacattg
     7321 cgaccgagat gctgatccgg aaagaagaac acctttggct tttttgacca agcccattcg
     7381 agagcggctt gcgcattgga ggaggtgcaa actataccgc catttttgcc acaaaaggct
     7441 ttcagctcca gccgcgagtt aatgtaggtt acgggaatca actgatcccc ggtaagcgcc
     7501 tgaatttttc cccaggcggt tttaacattt tctatattag ccatctctgc cagtggacaa
     7561 ccggcatcta catttggtaa aaacaccttt tgagcggcgc ttttcaataa atcagctgtc
     7621 tctgccataa aatgtacccc gcaaaatacg ataaactttg ccttcgtttc agatgctcga
     7681 cgcgacaggg caaacgaatc gccgacggca tcggcttggg caacaatctg gtcacattga
     7741 taatgatgcg ccagaatgat tagttgggga ccaaattgag cccggatttt gccaatgcgc
     7801 cgctcaattt cttcctgaga tagacgtgcc agatcgggcg ggggatagga aacattaagg
     7861 ttcatctata ttgcaaatac tttctgataa tgactttttc aaaaattgac gcataagtta
     7921 cacactattt atgtcatttt cttcaagatg tttacggcga cgttcggagc ggcgcgcctc
     7981 gttttcgagc gaatggtcgg cctcttcagg gtaattacga aaaaattggg tctgccaatt
     8041 ctgatagtca ccgcttaaaa tcgcctgacg ggcacggctg accagctcca taaaaaagtg
     8101 gacattgtga agagtattca agcgcaatcc ggtcatttcg ccattcataa aaagatggcg
     8161 gagaaaagct cgtgaaaaat tacggcaagt ataacaattg caatccggat caggcggtgc
     8221 aaagtccatt cgatattgac tctgtttcaa tattatgcgc cctttccagg tataaagtgt
     8281 cccgttgcgg gcattgcgcg acggcaaaac acagtcaaac atatcaattc cccagccaat
     8341 agcgcgtaca atatcttccg gtttacccac tcccatcaaa taacgcggct ttttttccgg
     8401 caattgcggt gcaaccaaag ataatatttc aaacatctgt tctttaggtt caccgaccgc
     8461 caacccacca actgcataac cgtcgaaatc cacttcggtc aaggccttta cgctctccgc
     8521 ccgcaaatcg ggatataccg agccctgtac aattccgaat tgaaactgac gatgaccata
     8581 gagcggtttt aatttcagaa aatgctcccg cgaaaggcgc tcccagcgat gggtacgctc
     8641 cagagcttgt ctggcatatt ctcggtcgca gggatagggt gtgcactcgt cgaatgccat
     8701 cacaatgtca gcgccgagcg ttcgttgaat ctccatagaa attgctggag atagacggtg
     8761 ctccgaaccg tcaatatggg agcggaatac aatctcatcc tcagtcaatt taccgagatg
     8821 agccaggctg aacacctgat aaccgccgct gtcagtcaat attggtcccg gccaggcatt
     8881 gaagcgatgc aaaccgccag ccgcccgaat tacatcgagt cccggtcgta agtaaaggtg
     8941 ataagtattg cctagtataa tttgaacgcg gcactcctgg agttcagccg gcgtcagagt
     9001 cttgactact ccgcttgttc caaccggcat aaaaaccggc gtttcaaccg tcccgtgatc
     9061 agtaacaaat atccctgccc gggctttggt accggtatct tgggcaataa tatcaaacgg
     9121 cagtttattt ttctctttag attcctttcg gccgtgattt gacatatcaa tcttccgaac
     9181 ttagaaaata aatccgaata gttgatttag cgattcgatg ccggttactt tggattgcag
     9241 gcttttttct aaaattttta tccccgactt gggtacatag accgtggtaa aaccaaactt
     9301 gagcgcctcg cgtaccctat tttctaaaaa tggaacactg cggacttcac caactaatcc
     9361 aacttcaccg atgaaagcgg cttgcggttc gagtgggaca tctttaaagc tcgacgctac
     9421 cgccaccgtc acacccaggt caattcccgt ctcatcaatt ttaaaaccac ccgccagatt
     9481 aatgaaaaca tcctgagtgc gcaaaggcaa accggcgcgt ttctccagaa cagctaccaa
     9541 catcacaaag cgtcggtaat caatcccgtt tgccgaacgc tgcggtgtgc cgtaagacga
     9601 ttgcgtcacc aatgcctgag cctctatcag aaaagggcgc gtgccttcca tactgaccgt
     9661 gaccgccgag ccagcaacgt attcacgacg ctccgttaaa aagaaaccgg ctgggtcgtc
     9721 aacggctatt agcccacgct ctttcatttc gaatattccg acttcattgg tcgaaccaaa
     9781 gcgatttttg accgcccgca aggtgcgata aaaattattc ttattgcctt ccagatataa
     9841 aaccgtatct accagatgct ccaaaacctt tggcccggca atcataccat ctttggtaat
     9901 atgaccgaca aaaacagtgg ttatatttaa ttccttggaa agacgcagaa gtcgcccggc
     9961 gcattcgcgc acctgagtta cactgcccgg gacattttca aattgatcgg agtaaacggt
    10021 ttgaatcgaa tcaattacca ccgcggtcgg cttcaggttc tctatttgat ctaaaatatt
    10081 ttccagatta ttctcacagg ctaccagaat acggtcatca ttaatttcca gtcgccgagc
    10141 gcgcaaagcc aattgctctt cggactcttc gccagagaaa tagagtaaac ggtcccccgg
    10201 cagtcgggcg atgacttgca taattagagt tgatttgcca atgccgggct caccgccgat
    10261 taatactacc gaaccactga cgaatccgcc gcccaacacg cgatcaaact cggttatgcc
    10321 ggtaaccaac cgctgggtcg aggtggtact tgcctgatta agaggcacca aattacgccg
    10381 agttctctcg acagcggagc tcctcggttt cttctgaatt tgttcagcaa ccatcgaatt
    10441 ccaggcgcca cattccggac atttgcccag ccatttaggc tgaactgaac cgcactcatt
    10501 gcatacaaag acatttttga ctttaacggg catgggtact ccttccgttt tttattttca
    10561 atatggctaa gtatttatcc ttgtcgtaca aaaatagtcc gaagaaaata atgagcatcc
    10621 caccaatttc acgccaggta acgatttcat tcaaccacaa tgctccgatc aaggtcgcaa
    10681 atatcggcga aatgaaatta ataaaagtca gcttgatcac attaatttgc ttcaaaagat
    10741 caaagtaaat ataaaatgcg agcgcggaac cgaacagacc aagataaacg atcgccccga
    10801 tatttttgaa gtcccagacc attttttgcc attcgccgcc gaccgttgct cctataaaat
    10861 tcacaagtgc ggcaatcaac attgccatag cagtcatcgt caaactatca taaggttgag
    10921 gacgcgtctt gagtaaaaca ttcggccagg aagatacgac taccccaaat aaggcaacca
    10981 cactgcccca taataattct cgaccaaaaa ccagctggcg atcggaaagg ataactatca
    11041 caccagccat cgctattaaa atcgccatca cctgggctaa gcgcaaacgt tcatctggta
    11101 acataaaatg agcaaatatc cccacaacaa tcggcatagc tgtccataag attgaagtaa
    11161 gcccactcgg gataaatttt tccgcccaat aagtgcagta aaaactaagc gccatagtca
    11221 aaagaccaac taccaagtaa ttctgtaccg atacacggtc aaaggcaatc ttacggcgtt
    11281 tcagaaaaat aataataccc agcgcgattg tcgccacgct gaaacgcaac gccagtccga
    11341 ccataagggg ggtttgatcg agaccaatct tgattaccgc ccaagtagtg ctccagataa
    11401 aacaaataag aatcaatttg aaatatttca tattcgttct gaataattaa taactcgcta
    11461 aactaattag cgtcagtatt aagaccaagc ctttttaaaa actagcttaa aaatttattt
    11521 aaaagtcagt atcgagacag aaataccacc tcggactata agtccggtcg aaatcaaacc
    11581 gccaggcgac gtcaatcttt aatacaaaaa ctcccaaata aatgcgcgct cccataccat
    11641 atccagctac caggtcatcc atcataaatc cacgatagta atcattcttg aagggatgaa
    11701 atttattatc ataccaggtc gaaccgaaat ctaaaaaagt tacgccttga annnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgaaattta ttatcatacc aggtcgaacc
    11881 gaaatctaaa aaagttacgc cttgaatccc acccatcgtt accggtagcg gccagcctaa
    11941 cacaagttgc ctaataaacg ggtagcggaa ctccagatta aacagaaaat agcgattacc
    12001 ggctctttca taatacagag cccctctcaa tggcgtaata aaatctgaaa aatagatgtt
    12061 ctcagcaagc gtcgagtcag cggtgaaaat atcataacgg taataataat ttatccaatt
    12121 atcctcgcca ccgagaaaga aacgctgagc atttttacca ttactgaaac cggtcgagag
    12181 gcgcaaaccg aaagaataat ccatgtttaa ttttaaataa cgacgaatat cagcttgaac
    12241 actataaaac tccagacttt tggcgctgta cttcgggctg gcaataaaat ccacccgcgc
    12301 ccgccaaccg tcaatcggat atagataacc ccatagtgaa ttatcataaa caaaaccgac
    12361 ccgtggcaaa attgtgttaa ttctatatgt gtataactct tctccggtta gagtcaggat
    12421 atatttctgc tcgacattat aggaagtcag ccctagctca gtccgcgaaa agtgactcga
    12481 aggacgcgat gcggaaaaat caagtccata atttcttaaa cgaaataccg taaaaaatcc
    12541 ataattccaa aagttcgacg tatgaaatcc ggttatgccc cagttggtac gcttggcaag
    12601 atacatatat gataaataat aatcactgtt ttccagatct atatacatct cggttcctaa
    12661 tgccagctga tgattaccta aaacatcact gaaataaaaa accgtagtgc cttgcaagcc
    12721 ccagaggtta ctatatccag cctcgccata agccacatcc agcgaaaagt tggttttgta
    12781 cggaaagacc gggtatctgc cacttttggt ggtttgtttg gtagtatcct cgctcgccat
    12841 agttgaatcc ggaaaatctt cttcaggctc tgcgctgatc tcataattat acatcggtgc
    12901 aaatatataa ttagagaaat ccccggcggc aattatattc tgtcgtgttc ggggtttcgc
    12961 ttcttcttta ctggggaaat tgaatctttg gtatttctcc ggattttccc aggattccat
    13021 taaagcttta gcgtaaactg ttggtttaat ttcctgacgt tcgtcccaaa gttgtaatgg
    13081 attacttatt gaataaatat cccagccttc atcggcatag ccagccaata ctaaacgcag
    13141 gtcatcattc gaaatactgg gctgaaaaac acccgtcaat acattggtaa tcgcaccgct
    13201 ctcgccggtg gatatatcca acataaaaat attggaaatc ccgtgataat cggaagtata
    13261 aaaaatagcg tccttggtat gcgcccagca cgggtaattt tcattccagg gcgaatcggt
    13321 cagacgcgta atttctccgt tatctaaatc aagacaaaat agatcggttt gaaaacattt
    13381 gacattcata atctctggta aattgggcag gctgtctatc tccggggttc gttgagaaac
    13441 gaatactatt ctctgaccgt ccggcgacca ggacggttca aagtcgggat atttatcatt
    13501 tatcagctgc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgctgacc
    13621 actgatcagc tttttaatta ccttgccgtc aaccgccgaa attaaataaa tatcggcgta
    13681 cccgttccga tcggtcatta tggcgatctt atcaccaagg ggtgaaatgg ctggtgcggt
    13741 attgaaataa tttttcaatt tcttatgatc ggtcaatttc atagaaatct cttcaatatc
    13801 ataccggtca gcaacgtccg gccaatattc agtgcgcatc caacgatgcc acttgtcagt
    13861 cagctctttc atatccatcc caatggcggc tttcaaaccg ttattggcat tacccttgct
    13921 tttcatttga gcccagattt gaccgatttt ctcgatacca tatttttcag caataaaacg
    13981 atatactgag ttgccgccct gataagccga gtaataatcg agttcctcga tattgggaat
    14041 attgctattc agagcaatat cacgcattat caaatcggtt tgagccgtcc agccgtaggc
    14101 aatatattca gccaagcctt cactcatcca atcaggaata ctcaatctca ctcgaccaga
    14161 gaccacactc tggacatttc cgccataaat catatcgttg atgaccacgt gcaccagttc
    14221 gtgccggatt acgtgccaga gatcgaagta cgagccttcg aaaggtacca ccgcacgatt
    14281 cttgaaaagt tcggtaaacc cgcctatccc ttcgtataaa tatgaaaggg ttacattggt
    14341 ctgttgaaaa tcgctgtgcg aattatatac gattaaagtc actcgcttgc gcaagcgcca
    14401 gttcagcact ttgcaaatgt ccttcagagc ttgttcccca accggagccg cgaactcggc
    14461 caaagattct ccgccttcat aaaaataaat atccatattc tcggattgca aatatgacca
    14521 attgaatgta tcgtactgaa ctttattttt accgtattgt gaaaatccgg ccgtcgcgaa
    14581 aatcagtatt aaaagaatgg atttaagttt ctttatcata gaattttcaa caaattatca
    14641 atagcgttgt tccaatctga atcaaaagta atcatttttt cgaccctcgt caagttaggc
    14701 tatcataaca aactttagaa gattaaatcg ggcaaaagtt aatcaactgg ctttcccaag
    14761 tttattaaca attattaagt agatggatta cctttctcct taatgaattt ctcaatccgt
    14821 tccttgagaa tattacgggt aatcctaaaa gccattaaac gccgctcttc atttccaatg
    14881 actcggatcg gatcatctat cagccattct aggcgctcag cctggccggg aaaagtcggg
    14941 caattcaaag cggcaacctc acataaaaca attacgtagt cgaagttata atcgcgatac
    15001 atgtcaatgt gtttggaata ctgattgtca accgagatac cgatttcggc taatacctgt
    15061 ttggtaaaac ggtgaattcc gaccggcgtg gaaccggcgc tgaaaacctc aaactgatca
    15121 ccgcctaact gacgcagaag agcttccgcc atttgactcc gcaccgaatt cccggtgcag
    15181 atgaagagaa ctgatttctt aaccatttaa ctgacttcca atagattcga cttactctta
    15241 aaattcaaat taacgaccga atatattaat tttttccgaa caataatact ctgtatgtgt
    15301 gttaatcggt aaatttttaa aaacttgaac aaataacaaa ttatttttcc ttcgttggac
    15361 aaaacaagcg ttatttttat tccagaattt taaaaccatt ctttttttcg tatattaacc
    15421 tcaaatatta tatttgaatc tggtaatgtg aattaataag ttgacattaa attgttccaa
    15481 agtaataact ataaacaaaa aaatattacc catctgagga ggtggcttga agagacgcga
    15541 atttatcaaa aaaagtggtg tgatagcggc tggaatcgtt acggctccag cgtggtttga
    15601 tatggcaggt ttttctaaaa ccgtatccga gacgcaagat ctggaaaacc tttttaacat
    15661 atcggcggtt gaagctgaga aacttctccg gctggcactt tcccgtggag cgcaaagcgc
    15721 cgatctattt tttgaatacc gtcccaattc cagtctgcgt attgaggatg gaatcattaa
    15781 agatattcaa tatggcattt ctctgggaat gggtgtccgc gctgttaaag gcgatcaaat
    15841 cggttacgcc tacaccgacg ctattgactt tcaaagtatg aaacgagcgg ctgaaaccgc
    15901 cgctaatatt gccaatagta cggggagttt taaacccgca cgtttccaga aagtagcggc
    15961 taaaaatttg tatccggtta aatcctatag tattgacatg gatctcaacc ccaaaataac
    16021 tatgctcaat gaagctgatg cgactgctcg acaattcaat gcgcgggtct ccagcgtaat
    16081 agcttcattt tctgaggaac tgcgccaaat tatgtacttt gactccaatg ggaaaaaatt
    16141 tcaagacatt caaccattaa tgagtctcag agtacaggtt atagctgaag atggcgttcg
    16201 gcgccagggt gcatctgctt ctcgctctgc ccgagcggga ctcgaatttt ttgaaaagcc
    16261 cgaaaatact ccatcggctg tcgctcgcca ggcggccgct actgccatta tcaatttaga
    16321 tgctaagcca gcaccagccg gaccgcaagt cgtaattctc ggcgccggtg aaagcggcgt
    16381 gctgatccac gaagccgtcg gccatggact ggaagccgat tatagctata aaaacctatc
    16441 taactacagc ggaaaagtcg gtcagaaagt agctgccgaa aattgcacta tcattgattc
    16501 aggcctcttc cctggcaaac gcggcgctct aaacgttgat gacgaaggca atttacctac
    16561 cactaccgtt ctgattgaaa acggtatcct caagggttat atgcataata gcatctcagc
    16621 ccggatgatg aagtctaacc ccaccggcaa tgggcggcga caatcttacg cttacccgcc
    16681 tctggctcgg atgaccacaa cttacctgca aaaaggcaac tacgaacccg aagaaatcat
    16741 cagctcggtt aaaaagggcg tttacgccaa gggcttcagc ggcgggcaag ttgatactat
    16801 caagggcgat tttacttttg gggtaacgga agcctatctg attgaagacg gcaaattaac
    16861 ggcaccgttg aaagacgtca ccttgattgg aaatggccca gatacaatga ataaaattct
    16921 gatggtcggc aatgacttga aaatttctga cggcacttgg tcttgcggca aagacggtca
    16981 aaatatccca gtcagcgtcg gtaatcccac agtcaaaatc agtgaaatca ccgtaggcgg
    17041 ctcttaatta cgaggaaaaa tatgcaagaa ttagtcaaaa tagccgaaaa tgttatctcc
    17101 gaggcgcgtc gcggtggcgc cgatgaggtt gacgtgctcg taaataattt caccaatacc
    17161 cggctcaaaa tccgcctcgg aaaagtcgag gaattgcgac agtccaatcc taaagcactc
    17221 ggcatccgtg ttttcaagaa taagcgtaaa gctctaacat acaccagtga cttgcgcccc
    17281 gaatcaattt ccaaaatggt acagaaagct ctcgaaattg tccgggtttc cagcactgac
    17341 gaatacaatg gattgccgga gaagcatttg tttggtaaag tgcaagctga tcttaagctt
    17401 tacgatcccg aaatcgctca aattcctacc gagcgcaaga tcaaaatggc taccgagctg
    17461 gaagatctcg gcctgaaaca agatccacta atcaccaact cgggcggctc attctggaac
    17521 gattcttatg gtcaatccat tctgatcaat tcacaaggtt tttccggcgt gcaggaattt
    17581 tctagctgtt cactttcttt acaactcgtc gccgaaaaag atggcgtcaa acaaaccgat
    17641 tactggtggt cgggtcaacg ggctttcaac cggctggatt cgattgaatc aatcgccaaa
    17701 acggcggccg cgcgcaccat ccgcaaaatc ggggctcgca aaccaaccac ccaatcagtc
    17761 ccggttgttt atgatcctca aaccggtgcc gatctacttg ctatacttgc tagtgttgta
    17821 ttaggcagtg ccatttatcg caaaaattcc ttcctggtag ataaactcta tcagcctatc
    17881 gccattgagg aattaaatat tattgatgac gctactttgc cgggcggtat cgattctcgc
    17941 tatttcgatg atgaaggtct gccagcccgc cgcaacattg tcatcgaaaa tggtattcta
    18001 aaaacttatc tatgcgactg ctactcagca cgtaaattaa aatgcactcc tacgggaagc
    18061 gcccgccgtg gcacaagcac tgacccctca ccgtcaacct cgaatttctt tctgccagct
    18121 ggaaaatatt caccagaaga aataatcggt tctgtcagca acggcttgta tctgaccgat
    18181 ttaaattggg ttggtattaa ttacgttacc ggagattatt cgcgcggcgc agaaggtatc
    18241 tggatcgaga atggcaaact tgcctatccg gttcaagaat tcaccgtagc tggcaatgta
    18301 ttagaaatgt taaaatcaat tagtatgata ggtaacgatc tggaattccg cggctcaatt
    18361 aattcaccga cattcaaaat tgagcggatg atggtcagcg gtcagtagga gtaaaagtaa
    18421 tgaaaatcgt agtttgcgtc aaagttgttc cggtatccga ttcgatgata accattgcac
    18481 cggaaaagac cgcaattgac ctggtcaaca ttaaattcga cctgaatcct tacgatgaat
    18541 ttgctattga agccgccgtt caattgaaag aaaaaaacgg cggcgaggta atcgccattt
    18601 cactcggacc cgagcgggat aatatcagct taaagcgcgt actggctctg ggcgccgacc
    18661 gtatgattca tctggtcacc gccgataaag cactcagtga ccccttagca actgctaagg
    18721 cattagccac agaaatcaag tcactctctc cagatttgat attctgcgga aagctttcca
    18781 ttgacaccgg caatggtctc gtcggacctt tggtagcccg tctgctcgga tatcctttcc
    18841 tgacaggaat atgcgaactg caggttggta cagaccaatt aacagccgct aaagacaccg
    18901 aagaaggccg aataatctac gagagttccc tgccttgttt attgactacc gaaaagggct
    18961 tgaataaacc gcgttatccc tcgctaaagg atattatgct ctctcgaaaa aaagagatta
    19021 ttaccaaaaa tgttacctta accgaaaagg cttccgaaat tattcaactg ttagaacccg
    19081 caccacggcc agctggtacg attctaggcc agaacgtgga tgcagtccca gaattgatcc
    19141 ggcgtctgcg cgaagaagcc aaagttttat aacggggaat cctgatgaaa atatttacct
    19201 atattgaacc agaaaacgga aatatctcca agaacagcgc gggctgtttg acacttgggc
    19261 gccaattggc tgatttagcc aatggaagcc tcactgctat tgtcccagct caggaaagta
    19321 gtactatgca aaatgaatgt ggtcactacg gagctgaccg agttgccttc actaaaaact
    19381 tcgctaaata cataccggaa gtaacggctt cagacgtagc caatatttta ctggaagaaa
    19441 atggggacat tctcattcta cctgaccgac cagctatccg tgacatcgcg gcaattgctt
    19501 gtgaaacggt tagcggttct atagccgttg attgtttaca agtcaatctc gactcagaaa
    19561 atagtcctat cgtnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggtcgtg
    19681 gcatcggtgc tccggaaaat tttcacttga tagaagaatt agccgacgct ctcggtgcgg
    19741 ccgtcggcgc cagccgttcc gttgttgatg ccggttgggt cccgcaaact tttcaggtcg
    19801 ggcaaaccgg taaaaccgtc tcaccagatc tatatattgc ttgtgggatt tccggcgcaa
    19861 tgcaacatat tgccggaata tcttccgcca aatacatcgt cgcgatcaac aatgatcccg
    19921 acgccccgat atttaaaatt gctaattatg gaattgtcgc taatcttttc gcaattatcc
    19981 cggaaatgat taagcaccta aaaaacataa aatgaataac ttagataata aacaaccttc
    20041 tgccgttatt gcagttgatg caatgggagg ggattttgcg ccggatgaag tagtgctcgg
    20101 tgccgctcgc gcttcggttg agactcccat aaaaatcatt ttagttggtg accaatctaa
    20161 aatagaacca attcttgaac aaactgaaca caatcctcaa cagctcgaaa ttgttcatac
    20221 cgaccaatat attaccaacg acgacaagcc gaaatttatc ctatccaaaa aaccgcgcgc
    20281 ttcaatgtta atcgcctcgc aactctgtgg tgcggatcaa gcacatggta tcgtctcggc
    20341 cggcaatacc ggtgcttata ttctttcagc gatcaagtac attccacgca tcgagggggt
    20401 tcacaaaact gccattgcct cagcctatcc tactcgcaac gaacagggaa ggtctgaccc
    20461 atttgccctc ttactcgacg tcggcgctaa cgtccattgc acagccgatg atctggtaca
    20521 gttcgctata atgggaaaaa tatatgcttc cgccgtcaaa ggtatagaaa atccgaccgt
    20581 tggactactt aatatcggca aagaacctta caaaggtggc gatactctca ccgacgcttt
    20641 taatatgctt aattcaatcc aagatatgaa attcataggt aatatcgaag gtaacgaaat
    20701 tttaaaaggt atagctgata tcatcgtcgc cgagggttat gtgggcaata tcgttatgaa
    20761 gactatcgaa ggttttggtg aaactatttc tcacatcgga cgctatgctt ttaaacaccg
    20821 ttttatctgg atgctcggtt tgcttgcgct ttccagtggc attaaacaat tgaaaaaggt
    20881 tgccgattat tcagaatacg gtggcgcacc tcttctcgga tttcgcaaaa tagtaatcaa
    20941 ggctcacggc cgtagtcgcg ccaaagccat taccaatgcc attatagttg ccgctaaaac
    21001 ttatcacgat aatgtttgtg aaaaaatggc tcgcgaaatt gctcaatatg aaaaacaatt
    21061 tcaacgtaaa aatgtaatga actgcgactg gaaattaaat ttcgaccaat aagatagcgc
    21121 ttggttagcc gttttatcaa gaccggttac caatttttta gtttggaagg agtaacttat
    21181 gaccaaattt tcactcgtca aaagtgcagc tgttttagga attgatgcct atatcgtgga
    21241 tgttgaatgt aatctcagta atgcgcaatt accggctttt attacggttg gattaccaga
    21301 gggcgccgtc cgtgaaagta aagaaagagt ttggtccgcg ataaaaaatc tcggctacta
    21361 tgtcccgagc aataaaattg tggtcaattt agccccagct gatataaaga aagaagggag
    21421 ctcctttgat ctaccaattg ctattggtat tctcactgcg gctgatttga tagagaaaca
    21481 taaggttagc gattacattt tcctgggcga actctccctt gatggaatgc ttcgcgaaat
    21541 taagggcgct ttgccaattt ccgtatcagt acgtaattct gggattaagg gcattatctt
    21601 gccggaacag aacgcccgcg aggcggctat aactggcgat ctggaaatta tcggcttaaa
    21661 atcccttcag gaagtgatag acttcctgga aaaccggact catccggaac caactcacgt
    21721 tgatgtccgg gcgctcttcg aagaacatcg ccaataccag attgactttt ctgaggtgaa
    21781 gggtcaggaa aatgtcaagc gcgccctcga agtcgcggcg gctgggggac acaatataat
    21841 tatgataggt ccgcctggtt cgggaaaaac gatgctcgcc aaacgtattc cgactatttt
    21901 gcccaattta actcttgaag aagcgctcga aactaccaaa atacattccg tagccggttt
    21961 ggttccctcc ggccagggca tcgtagccac tcggccgttc cggtcgcctc accacacgat
    22021 ttccgatgcc gcattggtcg gcggtggtaa attcccccgc cctggtgaag tcagtttgag
    22081 tcatcacggc gtacttttcc tcgacgaatt acccgaattt aaaaaaaacg tcctcgaagt
    22141 aatgcgtcag ccgcttgaaa atggctacgt aaccatctcg cgggcgctac tctctttaac
    22201 ctacccggct tcctttatgc tagttgccgc gatgaatccc tgcccttgcg gctatgccac
    22261 cgatcctcat catcaatgca cctgtacacc tcaacaaatc cagaaatata tggcgcgtat
    22321 ttccggtcca ttactagacc gaattgacat tcatatagaa gttcccgccg ttaaattttc
    22381 cgatctgtca gcgaaagcgc ccggcgaacc ttccgaaatt atccgggaac gtgtgcaagc
    22441 cgcccgccta aaacaaaccg cacgttttgc cgggatcaag ggtgtctttg ctaatgcgca
    22501 tatggaatca cgcttgatcc gaagattctg tcaaattgat caaacctgtg aagatctctt
    22561 gcgcaacgcc attaataagc tgggattgag tgccagagct tacgaccgga ttcttaaagt
    22621 atcccgaaca atcgccgacc taagcaattc cgaaaaaatt ttacccgaac acatcagcga
    22681 agctattcaa taccgcagtc tggatcgcca tctgtggtta taaaatttac taaatgtttt
    22741 gggacaattt ggctttcaga tcattaatct gacgagcaaa aaccgagtcg ctttgtaagc
    22801 gctcttccac ccagcggcaa gcgtgtacta ccgtcgaatg atcacgaccg ccaaaatata
    22861 aaccgatggt ttttagggat agatcggtga actctcgcgt taaatacatt gcaatcatcc
    22921 gcgcttcagc cacttctttg gtacgcccct tcccaaccat agaatccaga tttatcttat
    22981 aaacagtact caccgtactt tgaacactct ctattgataa caccgatgag tgacgagttc
    23041 ccagaatctc ccgcattact cgctgagtaa gattaatatc tatatccact tgcaataacg
    23101 aagaataagc taaaagccga atcaaagctc cttccagttc cctaatattc gaacgaatat
    23161 tggctgataa atattcaatt acttcatagg gaatgtctaa tccgtcatca ttagctttct
    23221 tctgcaaaat agcaatcctg gtttctaagt ctggcggctg aatatcaacg gttagactgg
    23281 attgaaagcg tgataagaga cgttcttgca aaccgcgcag ctccattggc ggcttgtcag
    23341 ttgttaatac aatctgctta tgcctttgat ataagtcatt aaaaatgtgg aaaaactgct
    23401 cctgcgtttg ttctttattt tgaaaaaatt ggatgtcgtc gaggagaagc atatcaatac
    23461 tgcgataaga acgagaaaat tcagtcgtct tgttgttctg aattgccgca atgaaatcca
    23521 aagtgaaggt atcgctggaa acatagacta ctttcttggc tctgccatta ttaagagctt
    23581 cattaccaat ggcttgaagt aaatgggttt t
//
LOCUS       DAOOMU010000029        22081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2287_length_22081_cov_22.290429, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000029 DAOOMU010000000
VERSION     DAOOMU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 22081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22081
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2287_length_22081_cov_22.290429"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PLI55_04285"
     rRNA            <1..252
                     /locus_tag="PLI55_04285"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            331..404
                     /locus_tag="PLI55_04290"
     tRNA            331..404
                     /locus_tag="PLI55_04290"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:365..367,aa:Ile,seq:gat)
     gene            442..514
                     /locus_tag="PLI55_04295"
     tRNA            442..514
                     /locus_tag="PLI55_04295"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:475..477,aa:Ala,seq:tgc)
     gene            609..3644
                     /locus_tag="PLI55_04300"
     rRNA            609..3644
                     /locus_tag="PLI55_04300"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3708..3824
                     /gene="rrf"
                     /locus_tag="PLI55_04305"
     rRNA            3708..3824
                     /gene="rrf"
                     /locus_tag="PLI55_04305"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            4009..5232
                     /locus_tag="PLI55_04310"
     CDS             4009..5232
                     /locus_tag="PLI55_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00090.1"
                     /translation="MKIISKILLFIILLISIVSAQDVNGVLDLRTNYFYNFEVYYLLD
                     FDFNNGRNNPDMFSYSLSYTPADGITPIQIKIEFEWIANIPAVNMYNHRILYVVTRPF
                     EFNGEVTLTTRDIDQNMHNIYYNDGRPCNISVDHFEQDLQRLQSIILSTGGKLPAGSY
                     VFTLNITDQYNTPLKSQTQVIEVSNPTTLELISPGGELADNFEIATTLPTFQWESIDF
                     MWSKTNCPQCGYAIRVAEYDPTRHNSVDQALKDRANLPFPDNNDYFPLPSSKISTVAG
                     LDLYTAGTIFQYPLTGAKPLEAGKIYVWQIRKTFPTTSGPESVLSPIFAFKIQSMGQT
                     SYTGIDVSQYLVLINQIPEINPEWFKEGGLLYGYVPTGTVILNGNQSITLEQLSVLVG
                     QLMTGQVSIKSITIE"
     gene            5244..5933
                     /locus_tag="PLI55_04315"
     CDS             5244..5933
                     /locus_tag="PLI55_04315"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPB00091.1"
                     /translation="MKKNILMKLLLALILFATSGFSQDKIALILKVRGETSLKRATDS
                     EFKKGVKIGDNLYSRDRIKTGKDGFTALVFLDDKSQIKVRENSEMEITGERSKGSISK
                     QVDMQFGTLKAEINPTKKGDFIIATPTSVASVKGTVFWVLSDPINGDVFYGISGSIEV
                     TNNESGGVIVVGANQTGISTPTGQVKVEDTPAGAQPVDEDAEESEENNELRIQLRNPS
                     GETKTIKIEYK"
     gene            5961..8573
                     /locus_tag="PLI55_04320"
     CDS             5961..8573
                     /locus_tag="PLI55_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00092.1"
                     /translation="MIELKNKSVRRIVINLSLILTLFSGLAYSQSKILHLPPREVYTD
                     SPVLIEAIIDQHTTEIREVKIYYRESGQEAFIEDYMTEIMGVFKYNIPAQFVTANGIE
                     YLIIAEFVDGSMAAFPEVDPFNVPMFVNARRMTSEPPIALDAVQTDLTGGNSSDVIIL
                     SPEEAQVVAASEVLIAVSLFNAKEIALHTVKIEVDGTNITPIAQIEEDLVLARPNNLP
                     PGNHTVTITLADPNGIYYDPLQWNFRVVGSVREAERVFNYNGRVLAEVNSEQVRGISQ
                     NIQQVTSGFNGSYDWIKFQANVFVTSEESPHKQPRNRYTAGFSSKFFEMNFGDINPQF
                     SEFGLNGKRVRGVEAHLKLNFFNLHVINGETERSIAGIISDTPQMLVSSGDTLYEYSR
                     TGYTYAQRLLAVRPYFGSGKHFQLGFNFIKAIDDTLSVHKEMNGIFAGGNQIIRMEGN
                     TPQDNIVIGSDLKLAFDNQRFVWKNAVALSMLNRNISGGALSKDDTLALGDFNIPIST
                     FGFDPSSIEKMFIINKYLYPISPIALDSVENVQNPIFKIQVDDSTQLAFGINEILNMP
                     SAAFRSTLNLNYFHNYITIKYQHIGSSFYSLGNPYMRKDIQGYLISDRIRLFQNKIFL
                     NINYEQNRDNLNNDKNATTTTSSFLAGISLFLGENLPRIDLNTVQYGRKNDVTNIDTT
                     WYGIPANSFSIKDNRESNITTRQDIRISHNINFLQIKNTLNFSWSSSDRSDRLAGSRP
                     EGYLFSSMKTSMITFSINSNFPFPLQTNLSISNNHSESGLNPEPYDFISIGAQGQYDL
                     FNKALGIYTGYGLTSGKGLADYTQNNIFGGFVFNFRKIHQLRSNVRYVYLDDRSSGEK
                     FHDLCYFVIYSLNF"
     gene            8589..>10790
                     /locus_tag="PLI55_04325"
     CDS             8589..>10790
                     /locus_tag="PLI55_04325"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPB00093.1"
                     /translation="MKINTLKQAIGGIIIALVLALLVSIFLVFGIFREMELKSLDARF
                     KNRGPKDISHSKLIIVAIDDQTFKSCRERYPYPREYYATLIENLEEVGIKTIMFDIQF
                     TEPDYKNPLGDSILADAIRKNGNVILAGKVTREFSVGGVNVYADKPLPILLETGSDWG
                     VVGEIQDPDGFTRRYPIFEQYQDKTIYTLGTKVFFKETGLPRVPEYKFENGYFIFTDS
                     TTGNTQQILAHRGSLGWEQTILINYYGPAGTFPTYSMSAIIDDSDFDLKDEEDTDYLE
                     MFKRNSTYPLEMRIALIADSTRQFEALAALADGDTASVLAILDEINPFKGKIALVGVS
                     IEELHDNKFTPFYNYGADRRLMPGVETHAHAIQTMLDQSFIRSLPITLNIILIFVISI
                     LTAVAVAFARPVLGGVLALILGFGALVLSYWTFRAHAVWVQLVPLLVATGTSYLSSVV
                     YQFLFEQKEKRKIRSMFQTYMSPKILKYLEDHPDAFSLSGEKREATMFFSDVANFTTI
                     SESLSAEELALVLNKYLSPMTEILMKYDGYVDKYEGDAIMCDFGVPMEDTDHAWKACF
                     AAIDQQAALEVLRPQIKKDHGVDIFVRMGINSGVVSAGNMGSNQRFQYTVMGDAVNQA
                     SRFEGANKQYGTYMMIGEETYRRAQDKIEVRILDRLVVKGKLKPITVYELLGRKGQVD
                     QEMLQICAYFTEGINLYWNRQWDAAIAAFEKALAVKGEDKPSRVFIERCHIY"
     assembly_gap    10791..10890
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10924..12492
                     /locus_tag="PLI55_04330"
     CDS             10924..12492
                     /locus_tag="PLI55_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HPB00094.1"
                     /translation="MSPKKGKILWVDDEIELLKAHILFLRSRGFEVVPASNGIDAINL
                     VSQENFDLVLLDEMMSGKDGLETLSDIKARKPGLPVIMITKNEEESLMEKAIGSKITD
                     YLTKPVNPSQVLMACKKILEQQRIASEHVSREYLEEFHVISQRLFNDLATNDWIEIYQ
                     KLARWEIEFDDHPDLGLNETLEDQKRDCNIEFAKFITKNYKKWVNDDPHFRPILSPDL
                     IPQKVLPLLQNGQKVLFLVIDCMRLDQWYTFAPLLYDFYNIDVDYYFSILPSATPFSR
                     NAIFSGLFPSEIQLLYPDIWMPDDEDETSMNRHEAVMLEKLLSEAEIDLRGKFNYMKI
                     LDSDSGWALEKRIASYLGNSLIALVLNFVDILAHRRVDSDFLKEILPNESSYRSVVRT
                     WFQHSWIYSVLRKFADSDFTIILTSDHGSIRVQNDVKVIADKTTSTNIRFKSGKNLNC
                     PPKYALNIKNPAEYKLPDLGINTNYLIAKENFYFVYPTNYHKFQTFFKDTFQHGGISM
                     EEMILPIVTMTPKK"
     gene            12496..12966
                     /gene="tsaE"
                     /locus_tag="PLI55_04335"
     CDS             12496..12966
                     /gene="tsaE"
                     /locus_tag="PLI55_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002668944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPB00095.1"
                     /translation="MTLQLVSHSAEDTIRLGERLATYLAPGTVVALIGELAAGKTTLI
                     KGICRGLGVTRPIESPTFTLVNEYPGSIYHIDCYREQRLMEWLELGINEYLYSDGITL
                     IEWADRIQQLLPETTLFILIRQDFNDDTVRYLEIQGSPELISQIANLQFVPNQL"
     gene            12998..13660
                     /gene="tsaB"
                     /locus_tag="PLI55_04340"
     CDS             12998..13660
                     /gene="tsaB"
                     /locus_tag="PLI55_04340"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPB00096.1"
                     /translation="MNSISPRLILGIETATLNCSVGLVHGNQIVGQKNILNRAVHSEK
                     LIELVDEVLKPDFTFADVNAIAVSIGPGSYTGLRIGLATAKGLAFYRQLPILPVPSLT
                     ALEATVGREEIGDSIFFIKSHRDLVYYTVVKKNEPLILKREIAYAPIGMVADLYPQHL
                     LIGDNAFDGQFGERLTVCFPMGEIVARLGADYFEDLLPLSRKAIEPDYHSNLEVKKWP
                     LK"
     gene            13645..14094
                     /gene="rimI"
                     /locus_tag="PLI55_04345"
     CDS             13645..14094
                     /gene="rimI"
                     /locus_tag="PLI55_04345"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPB00097.1"
                     /translation="MAAKIDARIRRANESDLTAILAIEEKSFTSAWTEDFFRHELYNP
                     VSRFYVLEKEKKIIGYIVIWLLADESHIANIAVHPEFRNAGLGGKLLNFAFEIARRNG
                     AKTITLEVNEQNVAARRLYEKFQFVLTGRRSHYYENKDDALILTRPI"
     gene            14105..14950
                     /gene="accD"
                     /locus_tag="PLI55_04350"
     CDS             14105..14950
                     /gene="accD"
                     /locus_tag="PLI55_04350"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HPB00098.1"
                     /translation="MADWFQRTKKGLLGTEKKVIPDGLWVKCPTCNEYLYKRELDKNY
                     WVCTHCSYHFRIRCEEYIKMLVGDKFEEFGLELQSDDPLKFKDSKKYTERLKQAMSQT
                     GSNEAVRTVTGQVDDVPVVLCVMDFNFIGGSMGSVVGEKIARAIDKAREMHLPLIIVS
                     QSGGARMQEGIFSLMQMAKTSAKLTRFHEDGGLFISILTDPTAGGTTASFAMLGDLII
                     SEPGAFIGFAGPRVIKQTIGQELPEGFQHAEFLLEKGFIDIISPRSELHQLVSKILKF
                     FYEPR"
     gene            14937..15719
                     /gene="surE"
                     /locus_tag="PLI55_04355"
     CDS             14937..15719
                     /gene="surE"
                     /locus_tag="PLI55_04355"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPB00099.1"
                     /translation="MSQDKPYILVTNDDGIAAPGINALALALKSIGEVIVIAPDSEKS
                     AVGHAITLSDPIRVTDFSKNGLWRGYSVSGTPADCVKIAVGALLERRPDLVVSGINLG
                     SNAGINVIYSGTVSAATEGTILGIPSIAISLATYSDPIFEPAAEFAIQISQLVLKKGL
                     PERTLLNINVPNVPKEQIRGIRITRQGMANFAELFDRRVDPRNRIYYWMDGKKAELEE
                     GEDVDDVAIRENLISITPVKYDLTNYEFIPELQNWLKEREEK"
     gene            15716..17257
                     /gene="guaA"
                     /locus_tag="PLI55_04360"
     CDS             15716..17257
                     /gene="guaA"
                     /locus_tag="PLI55_04360"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPB00100.1"
                     /translation="MSNISKVWIVDFGSQYTQLIARRVRDMSVYSEIVPSSVTALEAA
                     DQQVSALILSGGPASVNAVEAPQISPDFFEMDIPILGICYGLQLIARHFGARVHSDIH
                     REYGSSRIEIKKQHALFDGISTPTQVWMSHGDHVDSIPDDFEIIATSNNSVPAAMAHT
                     TRRIMGLQFHPEVEHTTEGKKILANFLFDIAGLRPDWTPEKFIEQTIADIRERTGTEQ
                     VLMAMSGGVDSSTMGVLINRAIGRRCIPVFINHGLLRQTEQIQVVRQLKDQMGLPIRS
                     YNYSHQFLSALKGVTDPERKRKIIGREFIRAFEEIARQFPNVKFLAQGTLYPDVIESR
                     SVNGPSQVIKSHHNVGGLPKRMNFQLIEPFSCLFKDEVRKIGRELGIPPEILGRHPFP
                     GPGLAVRILGEVTPRRLSILRRADAIYIEELRSSGYYDKVWQAFAILIPVKTVGVMGD
                     KRTYENALALRAVTSSDGMTADWARLPDTLLSRIANRIVNEVRGINRVVYDITSKPPA
                     TIEWE"
     gene            17273..>18588
                     /locus_tag="PLI55_04365"
     CDS             17273..>18588
                     /locus_tag="PLI55_04365"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HPB00101.1"
                     /translation="MKLSRTATFILVISLIWGSSLLADQTTDKLRSLLDEGIACYNAE
                     KYWDAQNIFKIINSIPKDENPYLTTSALMLIKTTYRLGEAEKSIALAYDFLQNYPESK
                     YVKEVKLTLAEVQLSLGNYQESLRIYLDLLKNYPDLEIQSQCEDYLNKIIDFYLEAET
                     VNALRDSTSDNFSRQYLTIKLAEKYHSEGHTDLAEAELKRLPARLENKYLRDQLQKVE
                     VQLKKPSVVRKYIGVILPLSGSNSASGREILNGVKYAIANYRKNNSEDIVALVFDNRS
                     DGVLSIKQAEFLARSPKVLAIFGPLMTENAVLVAVIANQYQIPMITPTASGSQLASLG
                     TYVFQANVDYENLGRFLGMYGAKVKDIKTVATIAPADEFGKEFTDAFCRAIDEFGGQV
                     ISQQWYQGEPTDLQYQINNLKMTAIEHATNHFEARFNLAKKQLVELV"
     assembly_gap    18589..18688
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18689..19071
                     /locus_tag="PLI55_04370"
     CDS             <18689..19071
                     /locus_tag="PLI55_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB00102.1"
                     /translation="RKNQAVLQNLYFISDYYIKTESDNYRRMAVDFKKNFGNEPGRME
                     LYGYDTMNALLTVIKSGAHTRESVRQGLLAMPVYHGLCRNISFQGNRPRINSCAFLLG
                     FKQNKIQPVAIIENGAIIEEKTKP"
     gene            19071..19847
                     /gene="pgeF"
                     /locus_tag="PLI55_04375"
     CDS             19071..19847
                     /gene="pgeF"
                     /locus_tag="PLI55_04375"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPB00103.1"
                     /translation="MFPLTVSKGIEIIQSPELMAFPEIVHAFSTRNGGISNGPFRSLN
                     LGIADGDTLKNILINRKRFYNTLNFTPSDVAQAEQVHSNKVAIVEDNGPIPGCDALIT
                     TRANLYLSIKTADCAAVLLYDPHHQVIAAVHAGWRGTAEGIIANTINTMQARFQTNPG
                     ELIAAIGPALHVCCYKVKNNVAKKFSDNEIIQREGNLFLDLILAITNRLNAAGVQLDR
                     IADSGYCTACQPEKFYSYRRDEGVTGRMLAVIGINQRNKC"
     gene            19861..20439
                     /locus_tag="PLI55_04380"
     CDS             19861..20439
                     /locus_tag="PLI55_04380"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPB00104.1"
                     /translation="MFNLIVAVIIGVCIFFGLRNGLIKSAFKLIGLVLAALCASRYYY
                     FGGKIIADLFNLSEGVQAVIGFVIVFIVVFLLFELLASMLRSLLRALKLIWVDRIGGM
                     LFGFLEGAIIMIGIVWVLNVYPELGFVDHLRQSSANFKFFERSGEKVIKICGLNSEMD
                     NLRKSMRKIVFLPETPQTNPENTNISPSENNP"
     assembly_gap    20612..20711
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20805..>22081
                     /locus_tag="PLI55_04385"
     CDS             20805..>22081
                     /locus_tag="PLI55_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HPB00105.1"
                     /translation="MNYARDQQWLHEDQATIRDGRLVLPLRVESKRKINGVIHGQSAT
                     GATVYVEPLEIVEINNALKELELAEKEEIERILRQTTDKMRPDFSPLQKNIQILAELD
                     LYNACARFGQKFKCTPPIIAENNDFVLRQARHPLLALHKEVVPLDFELANDVRAVVIS
                     GPNAGGKTVAMKTVGLLSQMAMSGLPIPAEEESRLPLITRFLADIGDQQSLENDLSTF
                     SSHINNMKYFLETADDRTLVLIDELGTGTEPTEGAALGQAILTELIAAGSLIIVTTHH
                     NALKEFAQKQPGAINAAMEFDMVTLAPTYRLQIGLPGSSYAFEISNRLGLPAKVVDNA
                     RQIVGEESVRLTNLLREVEQLRSEVEKDKKIISQNKKTLDKLVTEYNNKLNTLREKAE
                     RADTELARQLEELVNESRRRIENAVKEIREQAAT"
BASE COUNT         6144 a         4541 c         5118 g         5978 t
ORIGIN      
        1 agttcggatt ggagtctgca actcgactcc atgaagtcgg aatcgctagt aatcacggat
       61 cagaatgccg tggtgaatac gttcccgggc cttgtacaca ccgcccgtca cgccatggaa
      121 gtcggtaaca cccgaagtcg ctgtcctaac cccgcttcgg cggggaggga ggtgccgaag
      181 gtgagactga tgactggggc gaagtcgtaa caaggtagcc gtaccggaag gtgcggctgg
      241 atcacctcct ttctaaggag agtcccgatt cgtcgggata ggtcgaaaca ccagtctaaa
      301 atcaagcact ttctcttttt tgtcaattaa gggcttatag ctcaggtggt tagagcgcac
      361 gcctgataag cgtgaggtcg atagttcgag tctatctagg cccacgtcta aaaggctttt
      421 tctaatatcg aagctccgca aggggctata gctcaactgg gagagcgctg cccttgcacg
      481 gcagaggtta ccggttcaaa cccggttagc tccacaaatc ccgactagat cgggacatgc
      541 tttttgacat tgggataaag ggaaaagaga aaggatacaa gaagtccact tcataagaat
      601 agacttttgg tcaagatact aagggcaaat ggtggatgcc ttggcatata gaggcgatga
      661 aggacgtggc aagctgcgat aagctccggg aaggtgcaaa caactgtaga tccggagatg
      721 tccgaatggg gtaacccttc cgctttaata ggcggaaact gccgactgaa tccataggtc
      781 ggtaagagct aacggagaga attgaaacat cttagtaact ccaggaaaag aaaacaaacg
      841 tgattttcct agtagcggcg agcgaacggg aaagagccca aacttgttct atgttaaagc
      901 ctgccggcgt tgtagaacag gggtagcggg gcgttttcgg agagtgcggc agaacactcg
      961 acaagtcaaa aatctgtaaa ctattcaaac tactctggaa aggtagaccg tagaaggtga
     1021 tagtcctgta gaagaaagtt tgcagacttg ttggaagacg tacccaagta ccatggaaca
     1081 cgagaaactc tgtgggaatc tcccaggacc atctggaaag gctaaatact cctatatgac
     1141 cgatagcgaa gtagtaccgt gagggaaagg tgaaaagtac tcctgacgga gagtgaaata
     1201 gaacctgaaa ccatttgctt acaatctgtt ggagtcccgc ctcggcggga tgacagcgtg
     1261 ccttttgcat aatgagccag cgagttgctc gtaagttgca aggttaatcc tgattattcg
     1321 ggaggagccg tagcgaaagc gagtctgaat agggcgtata gtaacttgcg gcagacccga
     1381 agcggggtga tctatccatg gccaggatga agttccggta acacggaatg gaggtccgaa
     1441 cccaccagcg ttgaaaaact gggggatgag ctgtggatgg gggtgaaagg ccaatcaaac
     1501 tccgtgatag ctggttctcc ccgaaagatt tttaggaatc gccttgcata atagtgtatc
     1561 ggaggtagag ctctgaatgg gctagggacc ttaccaggtt accaaaccca accaaactcc
     1621 aaatgccgat tacatgtttt gcaggagtta ggctacgagg gataagctcc gtggccgaga
     1681 gggaaataac ccagatcaat agctaaggtc cctaaataca gggtaagtga ctaaggatgt
     1741 gaaaccgctt agacagctag gatgttggct tagaagcagc catcatttaa agagtgcgta
     1801 attgctcact agtcaagtga ttttgcgccg ataattagcg ggactattaa ccctgatacc
     1861 gaagctttga ttctctcgct tatggcgagg gaaggtaggg gagcgttcca ttaaccaatg
     1921 aaggtcaatc gcgaggttgg ctggaggaga tggaaatgag tatgctggca ttagtagcga
     1981 taattccggt gagattccgg aacaccgaaa gcctaaggtt tcctgagtaa agtaactctg
     2041 ctcagggtta gtcgggccct aagccgagcc ccgaactatc gggggtaggt gatggataac
     2101 aggttgaata ttcctgtacc atctgtttgc cgtttgaact atggggtgac gcagaagtga
     2161 aaggtcagcc gggtgttgaa tgtcccggtt taagcaggta gagggatctg attggcaaat
     2221 ccgtcagatc ataaccttga gatgcgaata ggagtccctc ggacacaaac tgaccctaaa
     2281 ccagctgaca agaaaagcct ctatgttagg tgaacggatg tccgtaccat aaaccgacac
     2341 aggtgggcga ggagagtatc ctaaggtgcg cgagatagct ctggttaagg aactaggcaa
     2401 attggccccg taacttcgga atatggggtg cctcgagtaa gtgaagctta ctatttcgat
     2461 agacaggcgg agctgaaaga ggtcgcagtg aagaggtcca agcgactgtt tactaaaaac
     2521 acatgtctct gctaagtcgt aagacgacgt atagggactg acacctgccc ggtgctggaa
     2581 ggttaagggg acgagtcatc ccgcttcggc gggggaagct ccgaactgaa gccccagtaa
     2641 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
     2701 cccgcacgaa tggtgtaacg atttggacac tgtctcgacc agagcctcgg cgaacttgta
     2761 atgccggtga agatgccggt ttcccgcagc tggacagaga gaccccggaa cctttactat
     2821 agcttggcat tgggttttgg gtttatatgt gtaggatagg tgggagacgt tgaagtcccg
     2881 acgccagtcg ggatggagtc aaccttgaaa taccaccctt ataaatctag aattctaatc
     2941 ccgacccctg aagcggggcg ggagacattg tcaggtgggt agtttaactg gggcggttgc
     3001 ctcctaaaat gtaacggagg ctcccaaagg ttccctcagc acgtttggta atcgtgcgcc
     3061 gagtgtaaag gcataaggga gcttaactgt gagacaaaca agtcgagcag atgcgaaagc
     3121 agggcttagt gatccggtgg ttgcttatgg aagcgccatc gctcaaagga taaaaggtac
     3181 tccggggata acaggctgat ctcccccaag agttcatatc gacggggagg tttggcacct
     3241 cgatgtcggc tcgtcacatc ctggggctgg agaaggtccc aagggttggg ctgttcgccc
     3301 attaaagtgg cacgtgagct gggtttagaa cgtcgtgaga cagttcggtc cttatccgct
     3361 gtgggcgcag gagatttgcg gggggctact cttagtacga gaggaccaga gtggacgaac
     3421 ctctggtgca ccagttgtgc cgtcaggtgc atcgctgggt agctatgttc ggtcaggata
     3481 aacgctgaaa gcatataagc gtgaaacctt ccccaagatg atatctccct tccgtttcgg
     3541 cggaactaaa ggcttcccgt agactacggg attgataggt cacaggtgta cgatcagtaa
     3601 tgattttagc cgagtgatac taataagccg tgcgtcttga ccaaaatttt tatgaaattg
     3661 acttctctaa tcgttctctt ttccttaacc caattaaaat ccttttttcc ggtgataata
     3721 gcggagggga aacacccgat cccatctcga actcggaagt taagccctcc agcgccgatg
     3781 atactgccct ggcaacgggg tggaaaagta ggtcatcacc gggaaaatct ttaatcccgc
     3841 ttcggcggga tttttattgt atctcgtctc ccctagtgtt atatttatcg cgtgttctca
     3901 ataattagaa gtagttttag atgaaagtga agaactaacc cttgttctat ctcacaatgt
     3961 cattaattca atatctttag attgaagcat gaataaaatg gaggatttat gaaaataata
     4021 tctaaaattc ttctgtttat aatcctgctg atttcaattg tatccgctca ggatgttaat
     4081 ggagtcctgg accttagaac caattatttc tataattttg aagtatatta tctacttgat
     4141 tttgatttca ataatggccg caataatcct gatatgttct cctattcgct ctcttatact
     4201 cctgcagatg gaattacgcc gattcaaatt aaaatcgaat tcgaatggat tgccaacatt
     4261 ccggcagtaa atatgtataa ccatcggata ttatacgtag tcacccgacc ctttgagttc
     4321 aatggagaag taactctgac gactcgggat attgaccaaa atatgcacaa tatttattac
     4381 aatgacggaa gaccatgtaa tataagtgta gatcatttcg aacaggattt gcaaaggttg
     4441 caatcaataa ttctttcaac tggtggaaaa ttgccggctg gaagttatgt gtttacatta
     4501 aatattaccg atcagtataa taccccccta aagagccaaa ctcaggtgat agaggtttcc
     4561 aatccgacaa cgctggagct gatttcgccg ggaggagagt tggcagataa tttcgaaatt
     4621 gctactacct taccgacttt tcagtgggaa tcaattgatt ttatgtggag taaaactaat
     4681 tgcccgcaat gtggctatgc cattagagtg gctgaatatg atccaaccag acataattcc
     4741 gttgatcagg ctttgaaaga tcgcgccaac ttgccatttc ccgataataa tgattatttt
     4801 ccattaccat catcgaaaat ctccactgtc gccggattgg atttatatac cgctggcacg
     4861 atcttccagt atccgctcac cggggcgaaa cccttagaag ccggtaaaat ctacgtctgg
     4921 cagattcgga aaacatttcc aaccacttcc ggacccgagt cggtcttgag cccgattttt
     4981 gcttttaaga ttcaatcaat ggggcaaacc agttacaccg gaatagatgt atcgcaatat
     5041 ttagtcctga taaatcaaat acctgagatt aatccagaat ggtttaaaga aggtggctta
     5101 ctatatggtt atgttccaac tgggacggta attttaaatg gtaatcaaag tattacgctc
     5161 gaacaattgt cagtcctggt tggccaatta atgaccggtc aagtttctat aaaatctata
     5221 acgattgaat aggatctggt aacatgaaaa aaaacatttt aatgaaatta ttgttggctt
     5281 taatattatt tgctacaagc ggattcagtc aagacaagat tgccctaatt ttaaaagttc
     5341 gcggcgaaac cagcctcaaa cgtgctaccg actccgaatt taaaaaagga gtaaaaatcg
     5401 gcgataatct ctattcacga gacaggatca aaaccggaaa agacggattt actgcactgg
     5461 tttttctcga tgataagagc cagataaaag ttcgagaaaa ttccgaaatg gaaataaccg
     5521 gtgaacgaag caaaggttcc atttcaaaac aggttgatat gcaattcggg actctgaaag
     5581 cggaaatcaa tcccacgaaa aagggtgatt ttatcatcgc taccccgacc tcggtggcat
     5641 cggtcaaagg aacagtcttt tgggtactat ctgatccgat caacggcgat gtcttttatg
     5701 gcatttcagg tagtattgaa gttaccaata atgagagtgg tggggtgatt gttgtgggtg
     5761 ctaaccagac cggtatttcg actccgacgg gccaagtcaa ggtagaagac accccagccg
     5821 gtgcccagcc tgttgatgaa gatgccgaag aatctgaaga gaataatgaa ctacgaattc
     5881 aattgcgcaa tccttctggt gagaccaaaa caattaaaat agaatataaa tagataatat
     5941 cggatcgagt ttttaaatac atgattgagc ttaagaataa atcggtacgc cgaattgtaa
     6001 ttaatttatc gttaatttta acgttgttta gtggattagc ctattctcaa tccaagattc
     6061 tgcatctccc gccccgggaa gtgtacactg attcaccggt tttgattgaa gcgattatag
     6121 atcaacacac tacggaaatt cgagaggtaa aaatctatta ccgagaatcg gggcaagaag
     6181 cttttattga agattatatg actgaaatca tgggggtttt caaatataat attccagcgc
     6241 aattcgtaac tgccaatggc atcgaatatc ttataatagc cgaatttgta gacggcagta
     6301 tggccgcttt tccggaagtt gatcctttta acgtgccgat gttcgtcaat gcccgccgca
     6361 tgacgtccga acctccaatt gctctggatg cggttcaaac cgaccttacg ggcggaaatt
     6421 ccagcgatgt aattatcctt agccctgaag aagctcaggt tgtagcggct tcagaagttc
     6481 tgatagcagt atctttattc aatgccaagg agattgctct ccataccgtt aaaatcgaag
     6541 tggatggaac taatatcacc ccaattgctc agatagagga agaccttgtt cttgcccgac
     6601 ccaataatct accacccggt aatcatacag tcacaattac cttggcggat ccgaatggga
     6661 tttattacga tccattgcaa tggaatttca gggtagttgg cagtgtacgt gaagccgaaa
     6721 gggtattcaa ttataatggc cgcgtgctgg ctgaagtgaa ttcggaacag gtgcggggta
     6781 tttcccagaa tattcagcag gtgacgagcg gttttaacgg ttcctatgat tggataaaat
     6841 ttcaagccaa cgtctttgta acttcggaag agagtccaca taaacaacct cgtaatcgtt
     6901 acacagccgg tttttcctcc aagttttttg aaatgaactt cggagatatc aacccccaat
     6961 tttctgaatt cggtttaaat ggaaaaagag tgcggggagt cgaagcccat ttgaaactca
     7021 acttcttcaa cttacatgtt attaatggag aaaccgagcg tagtatcgca ggaatcatca
     7081 gcgatacacc ccaaatgctc gtctcaagtg gtgatacttt gtatgaatac tcgcgtactg
     7141 gttatactta cgctcagcgc ctgctggcag tgcgacccta cttcggttcc ggtaaacatt
     7201 ttcagttagg ttttaatttt atcaaagcca ttgacgatac tctttcggtt cataaagaga
     7261 tgaatggcat ttttgcgggt ggtaatcaga ttataaggat ggaaggcaac acacctcagg
     7321 ataacatcgt catagggtct gacttgaagc tggcatttga taatcaaaga tttgtatgga
     7381 aaaatgcggt cgcgctctcg atgttaaatc gtaatatcag tggcggggct ttaagtaaag
     7441 acgatacgct ggcgctcggt gattttaata tcccgatctc aactttcggt ttcgatccca
     7501 gctcaatcga aaaaatgttt atcattaata agtatctata tccaatttca ccgatagctc
     7561 tggattctgt ggaaaatgtg caaaatccta tttttaaaat tcaagtggat gattccaccc
     7621 aattagcctt tggaataaat gaaattctta atatgccttc cgccgctttc cgaagtacat
     7681 tgaatcttaa ttatttccat aattacatca ctattaaata ccagcatatt ggtagctctt
     7741 tttattcatt gggaaatccc tatatgcgca aagatattca aggctaccta atctccgata
     7801 gaatccgcct cttccagaac aaaatatttt taaacatcaa ttacgagcaa aaccgcgata
     7861 atttgaataa cgacaagaat gccacaacta caacctcatc ctttttggcc ggcatttcat
     7921 tatttctggg cgagaactta cccagaattg atttgaatac agtgcaatat gggcggaaaa
     7981 acgatgttac caacattgat acgacctggt atggaatacc tgcaaactca tttagcatta
     8041 aggataaccg tgaatcaaat atcacgactc gtcaggatat tcgcatttcc cataatatca
     8101 atttcctcca aattaagaat accctaaact tctcttggtc cagttccgat cggagtgatc
     8161 ggctggcagg tagccgaccg gaaggctatc tgttcagttc gatgaaaact tctatgatta
     8221 cttttagtat caacagtaat tttccatttc ctctgcaaac caatcttagc atatccaata
     8281 accacagcga gagcggtttg aatcccgagc cttatgattt catttctatc ggagcccaag
     8341 ggcaatatga tctttttaat aaagctctgg gaatttatac tggctatggt ttgacaagtg
     8401 gtaagggatt ggcagactac acccaaaata acattttcgg cggatttgtc tttaatttca
     8461 ggaaaataca ccaactgcgt agtaatgtca gatacgttta ccttgatgac cgaagcagtg
     8521 gcgaaaaatt ccatgacctt tgctattttg tgatctattc cctgaatttt tagatactta
     8581 agcctctaat gaaaataaat acgctcaaac aggctatagg aggaataatt atagccctcg
     8641 ttctggcgtt actggtttcg atttttttag tgtttggtat atttcgtgaa atggaattga
     8701 aatcgctgga cgcgcggttt aaaaatcgcg gcccgaagga catttcacac tccaaattaa
     8761 tcattgtagc cattgacgat cagactttca aaagctgccg tgaacgctac ccttatccgc
     8821 gcgagtatta tgctaccctc attgaaaatc tggaggaagt cggtattaaa acgattatgt
     8881 ttgatattca atttactgag ccggattata aaaatccgtt gggtgattcg attctggcag
     8941 atgctattcg gaaaaacggt aatgtcattc tggctggaaa agtcacgcgg gaatttagcg
     9001 tcggcggcgt caatgtttat gccgataagc ctttgccgat actgctggaa accggtagtg
     9061 actggggagt tgtcggcgaa attcaggatc ctgatggatt tacccgccgt tacccgattt
     9121 tcgaacaata tcaggataaa acaatctata ctctcggtac caaagttttt tttaaagaaa
     9181 cgggtctgcc tcgagtaccg gagtataaat ttgaaaatgg ctatttcatt tttaccgatt
     9241 caacgaccgg aaatacccag cagattttag cgcatcgcgg cagtcttggt tgggaacaaa
     9301 ctatcctgat caattattat ggaccagccg gcacctttcc aacctattct atgtcggcca
     9361 taatagatga ttccgatttt gatttgaaag atgaggaaga taccgattac cttgagatgt
     9421 ttaagaggaa ttcgacctac ccactggaaa tgcgcattgc attgattgcg gattcgaccc
     9481 gtcaatttga ggctcttgcg gctctggcgg acggtgatac cgccagcgtt ttggcaatcc
     9541 ttgacgaaat caatccattt aaaggaaaaa tcgctttagt gggagtatct atcgaggaat
     9601 tgcatgacaa taaatttaca ccgttctata attacggtgc tgatcggcgt ctgatgccgg
     9661 gagtcgagac gcatgcccat gctattcaga ctatgctgga tcagagtttt attcgttccc
     9721 ttccgattac actgaatatc atcttaatct ttgtcattag catactcacc gccgttgcag
     9781 tggcttttgc cagaccggta ttgggtgggg ttttagcatt aatattgggt ttcggtgcgc
     9841 tcgtattatc atactggacg tttcgtgccc acgctgtctg ggtgcaattg gtgccgctct
     9901 tggtagctac cggtaccagt tatttatcga gtgtcgttta tcaatttctc ttcgaacaaa
     9961 aagagaaaag gaaaattcgg agtatgtttc agacttatat gagcccgaaa atcctgaaat
    10021 atcttgaaga tcatcccgac gcttttagcc tttccggtga aaagcgtgaa gccacgatgt
    10081 tcttttcgga tgtcgctaat tttactacca tatctgaaag cctctctgcc gaagaactgg
    10141 ctttagttct aaataagtat ttatctccga tgacagaaat tctgatgaaa tacgacggtt
    10201 acgtggataa atatgaaggt gatgcgatta tgtgtgattt cggtgttccg atggaagaca
    10261 ccgatcatgc ttggaaagcc tgttttgcgg ctattgatca acaagcggcg ctggaagttc
    10321 tccgccctca aattaagaaa gaccacggcg tggatatttt tgtacggatg gggattaatt
    10381 ccggagttgt cagtgccggt aatatgggtt ccaatcagcg ctttcagtac acggttatgg
    10441 gcgacgcggt caatcaggct tctcgttttg aaggtgctaa taaacagtat ggaacgtata
    10501 tgatgatcgg tgaagaaacc tatcgccggg cgcaggacaa aatcgaagtt cgtattctcg
    10561 accgtctcgt cgtaaaaggc aaattaaagc caattactgt gtatgaactt ctgggccgca
    10621 aaggccaggt tgatcaggaa atgttgcaga tttgtgctta ttttacggag gggattaacc
    10681 tctattggaa tcgccagtgg gacgccgcca ttgccgcatt tgaaaaagct cttgccgtta
    10741 aaggagaaga caaaccgtcg cgcgttttta ttgagcgctg tcatatttat nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tattaacaat aaaaagagtg attgagtaat
    10921 tctatgagtc ctaaaaaggg aaaaatatta tgggttgatg atgaaatcga attgttgaaa
    10981 gcccatattc tattcttgcg tagtcgcggt tttgaggtag ttccggcgtc gaatggcatt
    11041 gatgctatca atctagttag ccaggaaaat ttcgatttgg ttctactgga cgaaatgatg
    11101 tcaggtaaag atggactgga gactctatcc gacatcaaag cccgcaaacc cggtctgccg
    11161 gtaattatga ttaccaagaa tgaagaagag agcctgatgg aaaaagcaat cggcagtaag
    11221 attaccgact accttaccaa gccagtcaat cccagccagg tgctaatggc ttgtaaaaaa
    11281 atactcgagc aacaacggat tgcttccgaa cacgtttccc gcgaatatct tgaagaattt
    11341 catgttattt ctcaacgatt atttaatgac ctggcaacca atgattggat cgaaatttat
    11401 cagaaattag ccagatggga aatcgaattc gacgatcatc ctgatttagg cttgaatgaa
    11461 actctcgagg atcaaaaacg cgactgtaat attgagtttg ccaaattcat cactaaaaat
    11521 tacaagaaat gggttaatga cgatccccat ttccgtccga ttctttcgcc ggatttgatt
    11581 ccgcaaaaag tgttgcctct tttgcaaaat ggacagaagg tactgtttct tgttattgat
    11641 tgtatgcggt tggatcagtg gtacactttt gcgccgttat tgtatgattt ttataatatt
    11701 gatgtggatt attacttttc cattctacct tcggctaccc ctttttctcg caatgctatt
    11761 tttagcgggc tttttcccag tgaaattcag ttgctttatc ccgatatctg gatgcctgat
    11821 gatgaggatg agacttcgat gaatcgccac gaagccgtga tgcttgaaaa attgttatca
    11881 gaagccgaaa ttgatctgcg agggaaattt aattatatga aaattctcga ttcggacagt
    11941 ggctgggctc tcgagaaacg cattgcctct tatctaggta attcactcat cgcgctggtg
    12001 cttaattttg ttgacattct ggcacatcgt cgggtagatt ccgatttttt aaaggaaatt
    12061 ttgccgaacg aatccagcta ccgttccgtt gtgcgtacct ggtttcagca ttcctggatt
    12121 tattcggtct tacggaaatt tgctgactcc gattttacca ttattttaac cagcgatcat
    12181 ggcagtattc gcgttcaaaa cgatgttaag gtaattgccg ataaaactac ttctaccaat
    12241 attcgtttta aaagcggcaa gaacttgaat tgtccaccga aatatgccct gaatatcaaa
    12301 aatcccgccg aatataaatt accggatctg ggtattaata ccaattacct catcgctaag
    12361 gaaaatttct attttgtcta tccgactaat tatcacaaat ttcaaacctt ttttaaggac
    12421 accttccaac atggcggcat ctcgatggaa gaaatgatat taccgattgt tactatgacg
    12481 ccgaagaaat agactatgac tttgcaatta gttagtcaca gtgccgagga cactatccgg
    12541 ttgggcgaaa gattggcaac ctatttagct ccgggaacgg tggtcgcctt gattggtgaa
    12601 ttagccgctg gtaagaccac gctaatcaaa ggtatctgtc gtggtttggg agtaacccga
    12661 ccaatcgaga gtccgacttt taccttggtc aatgaatacc ccggctcaat ttaccatatt
    12721 gattgttatc gcgaacagcg tttgatggaa tggctggaac tcggcatcaa cgaatatttg
    12781 tatagcgacg gtataacctt gatcgaatgg gctgaccgaa tccaacagct tctgccggag
    12841 actactttat ttattctcat tcgacaggat ttcaacgatg acacagttcg ctatctggaa
    12901 atccagggtt cgcctgagct gatctcccaa attgccaatc ttcaattcgt tcctaatcaa
    12961 ttatgaattc ctactttaaa actttgaact ttgaactttg aactctattt ctcccagact
    13021 gatcctcggt attgaaacgg caacgctcaa ttgtagcgtt ggtctcgttc atggcaacca
    13081 aatcgtaggt caaaaaaata ttcttaaccg cgcagtccat tctgagaagc taattgagtt
    13141 ggttgatgag gtactaaagc ctgattttac ctttgcggat gtaaatgcca tcgcagttag
    13201 catcggaccg ggctcgtaca ccggattgag aattggtctg gcgacggcta aagggttagc
    13261 gttttatcgg caactaccga ttttaccggt gccatcttta accgctttgg aagctacggt
    13321 ggggcgggaa gaaattggag attcgatctt ttttataaaa tcccatcgcg acctggtata
    13381 ttatactgta gttaaaaaaa atgagccgtt aattctgaaa cgagaaatag cctacgctcc
    13441 gatcggtatg gttgccgatc tttatcccca acatttgctg attggcgata acgcatttga
    13501 cgggcaattt ggtgaacgac tgacggtatg ttttccgatg ggggaaatcg ttgcccgact
    13561 gggtgcagat tattttgaag atttattgcc gttaagtcgt aaggctatag agccggatta
    13621 tcattccaac ttggaggtca agaaatggcc gctaaaatag atgcccgaat tcgtcgggct
    13681 aacgagtcgg atttaacggc tatattggca atcgaagaaa aatcgttcac atctgcctgg
    13741 acggaagatt ttttccggca cgaattatac aatcctgtta gtcgttttta tgtactcgaa
    13801 aaagagaaga aaatcatcgg ctatattgtc atctggttgt tagccgatga atcgcacatc
    13861 gccaatattg ccgttcaccc cgaatttaga aatgccggtt taggtggaaa attattaaat
    13921 ttcgcttttg aaattgcgcg tcgtaatgga gcaaagacga taaccctgga agttaatgag
    13981 caaaatgttg ccgctcgtcg tttatatgaa aagtttcagt ttgttttgac cggacgtcgg
    14041 tcgcactatt atgaaaataa ggatgatgct ctgatactga ctcgcccaat ttgatgaggt
    14101 aaatatggct gactggtttc aaagaacaaa aaaaggtcta ctcggaaccg agaaaaaagt
    14161 tatccctgat ggactctggg taaaatgccc gacctgtaat gagtatcttt ataaacgtga
    14221 gctcgataaa aattactggg tttgcactca ctgtagctat catttccgga ttcgctgtga
    14281 agaatatatc aaaatgttag tcggagataa gttcgaagaa tttggattgg aactgcagtc
    14341 ggatgatcct ttaaaattca aagattctaa aaaatatacc gagcgtttga aacaggcaat
    14401 gagtcagacc ggctcgaacg aagcggttcg aaccgtaacc ggtcaggttg atgatgtgcc
    14461 ggtggtatta tgcgttatgg attttaactt tatcggcggg agtatgggct cggtcgtggg
    14521 tgaaaaaatt gcgcgcgcta ttgataaagc ccgcgaaatg catctaccat tgataatcgt
    14581 gtctcaatcc ggcggagcgc ggatgcaaga agggattttt tccttaatgc aaatggctaa
    14641 gactagcgcg aaattgacgc gttttcacga agacggtgga ttatttatat ctattttaac
    14701 cgatccgaca gccggtggta cgacggcgag ctttgcgatg ctcggcgatt tgataatttc
    14761 cgaaccgggt gctttcatcg gattcgctgg accacgagtc attaagcaaa ccatcggtca
    14821 ggaactgcca gaaggcttcc aacatgctga atttttactt gaaaagggtt ttattgatat
    14881 tatctcgcca cgttcggaac tccaccaatt agtcagtaaa attttgaagt ttttttatga
    14941 gccaagataa gccctatatt ctggttacta atgacgacgg catcgccgcg ccgggtatta
    15001 atgcgctggc actcgctctg aaatcaatcg gtgaagtaat tgtcattgcg cccgacagtg
    15061 aaaaaagcgc agttggtcat gccattactc tatctgaccc aatccgggtg acggatttta
    15121 gtaaaaatgg attatggcgt ggctattcag tctcggggac accagccgat tgcgtcaaaa
    15181 ttgcggttgg cgcgcttttg gaacgccgac cagacctcgt cgtttccgga atcaatctcg
    15241 gttccaatgc cggcatcaac gtaatttatt ccgggacggt ttcggcggct acggaaggta
    15301 cgattctggg aattccctca attgccatct cactcgccac ttactctgac ccaattttcg
    15361 aaccagccgc agaatttgcg atccaaatct ctcaactcgt tctaaaaaag ggcttaccgg
    15421 agcggacttt attgaacatt aatgtcccta atgtgccgaa ggagcaaatt cgcggcatcc
    15481 ggattacacg ccaggggatg gcaaacttcg ccgaactctt cgaccgaagg gtggatcccc
    15541 gcaaccggat ttactattgg atggatggta aaaaagccga actcgaagaa ggcgaggatg
    15601 ttgatgacgt ggctattcgt gagaatttga tttcgattac gcccgtcaaa tatgatttga
    15661 ccaactatga gtttataccg gaattgcaga attggctaaa agagagagag gaaaaatgag
    15721 taatattagt aaagtttgga ttgtagattt tggttcacaa tatacccaat taatcgcgcg
    15781 ccgggtgcgg gatatgtcgg tttattccga aattgtaccg tcgagtgtga ctgcccttga
    15841 agccgctgac cagcaagtaa gtgctttaat tctatccggt ggtcctgcca gcgttaatgc
    15901 ggtcgaagcg ccacagattt ctcctgattt tttcgagatg gatataccga tattggggat
    15961 ttgttatggc ttgcaactga ttgcgcggca tttcggagcg cgggttcatt ctgatattca
    16021 tcgagaatac ggttcatcta ggattgagat caaaaaacag catgctctat ttgatggtat
    16081 ttcgacgccg actcaggtct ggatgagtca tggcgatcac gtagattcaa taccagatga
    16141 ctttgaaatt attgcgactt caaataatag tgtacctgcc gcgatggcgc ataccactcg
    16201 tcggattatg ggacttcagt ttcatccgga agtcgaacat actacggaag ggaagaaaat
    16261 cttagctaat ttcctctttg acatcgccgg gcttcgtcca gattggactc ccgaaaaatt
    16321 tatcgagcag acgattgccg atatccgaga gcgaaccggc acggaacaag ttttaatggc
    16381 gatgagcggt ggagtggatt cctccacgat gggggttctg attaatcgag ccattggccg
    16441 ccgctgtatt ccggttttta tcaatcacgg gctactgcgc cagaccgaac aaattcaagt
    16501 cgtccggcaa ttaaaagatc aaatgggatt gccgattcgt agttataatt attcgcatca
    16561 atttcttagt gccttgaaag gagtaaccga tcccgagcgt aaacgcaaaa taatcgggcg
    16621 ggagtttatc cgggcttttg aagaaattgc ccggcaattt ccgaatgtaa aatttctggc
    16681 gcaaggaaca ctttatccgg atgtcatcga aagtcgctcg gtaaatggtc cctcgcaggt
    16741 aattaaaagt catcacaacg ttggtggtct gccgaaaaga atgaatttcc agctaattga
    16801 accttttagc tgtctcttca aggatgaggt tcgcaaaatc gggcgtgaac tcggaatccc
    16861 cccagaaatt ttaggacggc atccattccc aggtccggga ttggctgtcc gtattctggg
    16921 cgaagtcact ccccgccgac ttagcattct acgtcgagcg gatgccattt acatcgaaga
    16981 attacgttcc agcgggtact atgataaggt ctggcaggct ttcgcaatcc tgattccggt
    17041 taaaacagtc ggcgttatgg gcgacaaacg cacctatgaa aatgccctag ctttgcgtgc
    17101 ggtcacgagc agtgacggca tgactgccga ttgggcgcga ttgccggata cattactcag
    17161 tcgcattgct aaccgaatcg tcaatgaagt tcgcggcatc aatcgcgtcg tctatgatat
    17221 cacttccaaa ccaccagcta cgattgagtg ggaataaatt aagtttttga tgatgaaatt
    17281 atccagaacc gctaccttta ttctcgttat ttccctgatt tgggggtcga gcttgttagc
    17341 cgaccaaacg actgacaaac tgcgaagttt gctcgatgag ggaatagcct gttataacgc
    17401 cgaaaaatac tgggatgccc agaatatttt taaaatcatt aattccatac ccaaagacga
    17461 aaatccctac ttgacaactt ccgctttaat gctgattaaa acgacttacc gcctgggcga
    17521 agcagagaag agtatcgccc tggcttacga ttttctccag aattatccgg aatcgaaata
    17581 tgttaaggaa gtaaaactga ctttagccga agtgcaatta tcgctgggga attatcagga
    17641 atcactgcga atatatctgg atttattgaa aaattatccc gatctggaaa tccagtcgca
    17701 gtgtgaagac tatctcaata agataattga tttttatctc gaggctgaga cagtcaacgc
    17761 cctgcgtgac tcgacttccg ataatttttc gaggcaatat ttaacgataa agttagccga
    17821 gaaatatcac tccgaaggac acactgactt agctgaagcc gagctaaagc gattgcctgc
    17881 tcgtctcgaa aataaatatt tgcgcgacca attacagaaa gtcgaagttc agctgaagaa
    17941 gccgtccgtc gttcggaaat acattggagt aatcctgcca ttgtcgggct caaattcagc
    18001 ttccggtcga gaaattttaa atggtgtcaa atacgccatt gctaattatc gcaagaataa
    18061 ttccgaggat attgttgccc tcgtatttga taatcggagc gatggcgttc tgagtatcaa
    18121 gcaagccgaa tttttagcta gaagtcccaa agtacttgct attttcggcc cgctgatgac
    18181 tgaaaatgcc gttttggttg cggttattgc caatcagtat caaatcccaa tgatcacgcc
    18241 gacggcttcc ggtagtcaat tggcgtcttt gggaacttat gttttccagg caaatgtgga
    18301 ttatgaaaat ctcggacgtt ttctggggat gtatggagca aaagtaaagg atataaaaac
    18361 tgtggcgact atcgcgccgg cggatgaatt tggcaaggag ttcaccgacg ctttttgccg
    18421 tgcaattgat gagttcgggg gacaggtaat ttctcagcaa tggtatcaag gcgagccgac
    18481 cgaccttcag taccagatca acaatttgaa gatgacggcg attgagcatg ctactaatca
    18541 tttcgaggct cgcttcaatc tagcgaaaaa gcaattagtg gaattagtnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta aggaagaatc aggcggttct gcaaaatcta
    18721 tattttattt cggattacta tatcaagaca gaatcagata attatcgtcg gatggcggtt
    18781 gattttaaga aaaattttgg caatgaaccg gggcggatgg aattatatgg gtatgatacg
    18841 atgaatgcct tactcacagt aatcaagagc ggcgcgcata cgcgggaatc ggtgcggcaa
    18901 gggcttttag caatgccggt ctatcacgga ctatgtcgca atatttcctt ccaggggaac
    18961 cgcccacgca tcaattcctg tgccttccta cttggtttca agcaaaataa aatccaacca
    19021 gtggcgatca tcgaaaatgg tgccattatt gaagaaaaaa ctaagccata atgtttccac
    19081 taaccgtttc caaagggatt gaaatcatcc aatctccgga attaatggct tttccggaaa
    19141 ttgtccacgc tttcagtacc cggaatggtg gaataagtaa tggacctttc cgttcactta
    19201 atcttggaat cgccgacggc gatactctta agaatatcct gattaatcga aaaagatttt
    19261 ataataccct caattttact ccgtccgatg ttgctcaagc cgaacaagtt catagcaaca
    19321 aggtcgctat agtggaagac aatggtccta ttccaggttg cgatgctcta attaccactc
    19381 gagccaattt atatctgagt attaaaacgg ctgattgtgc ggctgtttta ttgtatgatc
    19441 cgcaccacca ggtcattgcg gcggtgcatg ccggttggcg gggcacagcc gaggggataa
    19501 tcgccaacac cattaatact atgcaggctc gatttcaaac taatccgggc gaattgatag
    19561 ccgccatcgg tccggctttg catgtctgtt gttataaagt gaaaaataat gtcgccaaaa
    19621 agtttagcga taatgaaata atccagcgag agggaaatct ttttctcgat ttaattttgg
    19681 caattaccaa tcgcttgaac gcggctggtg ttcaactcga taggattgcc gatagtggtt
    19741 actgtaccgc ttgtcaacct gaaaaatttt attcctaccg gcgggatgag ggcgttaccg
    19801 gacggatgct ggctgtaatt ggcattaatc agcgaaataa atgctaagtg atagattcgg
    19861 atgtttaatt taatagtagc cgtaattatt ggagtttgca tttttttcgg attacgcaat
    19921 ggcttgataa aatcggcttt taagcttatt ggcttagtat tggcggcgct ctgtgcttca
    19981 cgatattatt attttggtgg gaaaataatt gccgaccttt ttaatctatc cgaaggtgtg
    20041 caggcggtga ttgggtttgt tatcgtattc atcgtggtgt ttttactttt tgaattgctg
    20101 gcttcaatgt tgagaagcct tttacgagct ttgaaattga tatgggttga ccgaattggc
    20161 ggaatgctgt ttggattcct ggaaggtgcc attattatga tcggaatagt ctgggtatta
    20221 aatgtctatc cggaactcgg attcgtggac catctgcgtc aatcttcagc caattttaag
    20281 ttttttgaac gcagtggcga gaaagttatc aaaatttgcg gactgaattc ggagatggat
    20341 aatctccgta aaagtatgcg taaaatcgtt ttcttgccgg aaaccccgca gacaaatcca
    20401 gaaaacacga atatttctcc aagtgaaaat aatccctgaa ttaatgacaa tacctgaatc
    20461 tatgaaggaa accaacgata aactcggttt tgctaaaatc atcgagttga ttcaggaaat
    20521 ggctctctct actgccggca aatctctggc taatactatt aagccgctaa aagatattga
    20581 aattattcga tgccggcaaa gtcgcattaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20701 nnnnnnnnnn ntcgcttcgc gatagcgcca gtccgcagct taatcgaatt cgccatgaaa
    20761 ttcgcactca ccagcgtcgc ctcaatcatg agattgagcg tttgatgaac tacgcgcggg
    20821 atcagcaatg gcttcatgaa gatcaggcaa ctatccgcga cggacgtttg gttttgcctc
    20881 tacgagttga atcgaaacga aaaattaacg gagtaattca cggccaatca gccaccggtg
    20941 ccaccgttta tgttgaaccg ctcgagatcg tagaaattaa taatgctctt aaagagctgg
    21001 aactggctga gaaggaagaa atcgagcgta ttctacgcca aacaaccgat aaaatgcggc
    21061 cggacttctc gcctttacaa aaaaatatcc aaattctggc tgaactcgac ttgtataatg
    21121 cctgtgcacg ttttgggcaa aaattcaagt gcacgccgcc gattattgcc gagaataatg
    21181 attttgtttt acgtcaagcg cggcatcctc ttttagcgct ccataaggaa gtggttccgc
    21241 tggattttga gctggcgaat gatgtccggg cagttgttat ctctggccca aatgccggcg
    21301 gcaaaaccgt cgcgatgaaa accgttggtc tcctatcaca aatggcaatg agtggactac
    21361 caatcccagc cgaagaagaa agtcgccttc cgctcattac tcgattttta gctgacattg
    21421 gcgaccaaca atcgctcgaa aatgaccttt cgactttttc ctcgcatatt aacaatatga
    21481 aatattttct tgaaactgcc gatgaccgaa ctttagtcct gattgatgag ctcggtaccg
    21541 gtactgaacc gactgaaggc gcggctctcg ggcaagcgat tttaaccgaa ttgattgccg
    21601 ccggctcgct gataattgtt acgacccacc ataacgcctt gaaagaattc gcccaaaaac
    21661 aacccggcgc cattaacgcc gctatggagt ttgatatggt aacattggcg ccgacttatc
    21721 gtctgcaaat aggtttgccg ggtagcagtt atgcctttga aatttccaat cggcttggtc
    21781 tgccagcgaa agtggttgat aatgcgcgcc agatcgtcgg tgaagaatcg gtgcgtttga
    21841 ctaatctttt acgcgaagtt gaacaattgc gttcagaagt cgaaaaggat aaaaagatca
    21901 tctcccaaaa taaaaaaacg ctcgataaat tagtaaccga atacaataat aaactcaata
    21961 ccttgcggga aaaagccgaa cgggctgata ccgaactggc acgccaatta gaagaacttg
    22021 taaatgaatc gcgtcgccgg atcgaaaatg cagtcaaaga gattcgcgaa caagccgcca
    22081 c
//
LOCUS       DAOOMU010000030        21895 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2313_length_21895_cov_20.561905, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000030 DAOOMU010000000
VERSION     DAOOMU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 21895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21895
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2313_length_21895_cov_20.561905"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..570)
                     /locus_tag="PLI55_04390"
     CDS             complement(34..570)
                     /locus_tag="PLI55_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB00106.1"
                     /translation="MDFSELILKRRSVRKYLPKPVERELIDKCLDAARLAPSACNAQP
                     WYFIVIDNEEVKDRLCSAAFSGIYSSNKFVKNAPVIIVVITEQQSYQVKVGGFFRHLK
                     YALVDIGIAGEHLALQAAELGLGTCWLGWFSENGVRKALDLPKSTKIDIMFSLGYPAD
                     SDRPKKRRPLDEIRRYID"
     gene            complement(573..2273)
                     /gene="recN"
                     /locus_tag="PLI55_04395"
     CDS             complement(573..2273)
                     /gene="recN"
                     /locus_tag="PLI55_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPB00107.1"
                     /translation="MSLTKLKIDNFAIIDHIEVAFGPGLTILTGETGAGKSIIIDALN
                     LALGEKASPNVIRAGADVATVECTFDLTDLNPATKNILKSHNFYQENNPLTLKREVNI
                     NGRSRAWLNGETCLVSVLKEVGNLLVDMHGQHDHQSLLNPDTHIEFLDAFGDYAEWRE
                     QVARQFDELKLLFERQNLLQEQLRLNREKRELWEFQLQEIQKVAPQENEYETLLQEKT
                     ILENTAKIQQLSSDLTYQLLESGDSLYNQLQGVVKQLANLQKITGSFVEQLSRLEESQ
                     YLFQEIARQIAHFGDDLQFNPARLEIINQRLYSLQQLMKKYGPSLSDVVAYRQKLETD
                     LTESDDLEVQIDKNRELIKEHLEKFTRFAFELSEKRKQSAELFKIEIEKILERLGISG
                     ADFAVRLELMPDQNGWIIIDGQKYKAESTGIDKVTFEISTNRGEPRRPLAEIVSGGEV
                     SRIMLAIKTILAGRDHIPVLIFDEIDTGISGKIARKVGEELHELAKVHQVICITHLPQ
                     IAGLADEHFSVEKVTTNGRSQTQIRKLLPEERVAEIAKLIGGRSVSEITLKQARELME
                     "
     gene            complement(2270..2650)
                     /locus_tag="PLI55_04400"
     CDS             complement(2270..2650)
                     /locus_tag="PLI55_04400"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPB00108.1"
                     /translation="MNRFWLKVPAILYAVLIFSLSSLTKTPPIEIPIWNVDKLVHMLE
                     YTIFGILLVLAFRSDQIGKPMRRANFQSIILGVLYGLTDEIHQYFVPGRTSSLADLGA
                     DALGILLGVWIFNRLNVFQKFREA"
     gene            complement(2650..4206)
                     /locus_tag="PLI55_04405"
     CDS             complement(2650..4206)
                     /locus_tag="PLI55_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPB00109.1"
                     /translation="MPEFILSNAEASAIDRYAIEELQIPGTELMFKAGNFVALKAKKL
                     IQDIPNSRIDLFCGIGNNGGDGFVAALLLSEWGANCIIWLIGDPQNIKGDARHFYQKW
                     VESKQTIRCIKTAEDFEEYSDLPESDLIIDAMLGTGFRGEVQGIIADVIRRINNINLP
                     VIAVDIPSGVNGDTGEVRGDAVKADYTVTMGFLKRGLLFYPGKRFAGEIVVVDLGYPR
                     KAFDILKEKTFLIGKQDVKKVIPPIAEDTYKHRQGKVLIIAGSPGMTGAATLCALGAM
                     RSGAGLVVNAIPKSLNPIMEVKLTEALTAPLPESSQQTFCLESLTALQSRLEWSDVVV
                     FGPGVSDSFEVQEFGRQLITKCHKPLVIDADGLRIFHNNLELIGTIEDLTITPHRGEF
                     AMMTGIAVAEVNKNVIEVARDFVRKYPCTLVLKGAPSVVVSSTGVVAVNSTGNPALAT
                     GGTGDVLTGIIAAFRAQGLNSFDAAMAGTSIHGLAGDWGRKKLGVRAFIASDLLDYLP
                     LILRRLDRVI"
     gene            complement(4358..5647)
                     /locus_tag="PLI55_04410"
     CDS             complement(4358..5647)
                     /locus_tag="PLI55_04410"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00110.1"
                     /translation="MKYVKQISVIILLTLVVLSSLIGQDPLSEAYTLFQNGEYQKAFE
                     KCKPIVEKDPNYAGAVFLLGRIYFALGELDSSKVYLDKAIDLDRANTEFREIRTQIAA
                     FTTQLTEASRLVNNGDYEGAKNLYLEIIKANPNFADAYFNLAQVYIRLNDLTNASLYL
                     RKVIEMKPNEERYTKLLKNLVQQYLQEGENLRQRRNNEGALEKYKQALELDPNEFLAY
                     YFSGLAYYDMKNYDEAIKAINKGLEINPEHAKSYLLLGQIYEKTNKKAEALKAYEQAV
                     KIDPLFEAGWDRIGVLNYNMKNYEQSINAYKKLIEINPENPRYYEKLGQIYLDTNNPK
                     EAIINLEKVTQKDPKNVKAWLRLAQAYNNNGECAQAKTAAETALKIKAGDALALIELG
                     IAERCLGNTAAARQAFQLAAKDPQYRRYAEEHLKTVQ"
     gene            complement(5680..5765)
                     /locus_tag="PLI55_04415"
     tRNA            complement(5680..5765)
                     /locus_tag="PLI55_04415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5729..5731),aa:Leu,seq:gag)
     gene            complement(5813..6160)
                     /gene="secG"
                     /locus_tag="PLI55_04420"
     CDS             complement(5813..6160)
                     /gene="secG"
                     /locus_tag="PLI55_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPB00111.1"
                     /translation="MYTFLIIIHVIVSALLMLVILMQSSKGGGLSGTFGGAGGSSLFG
                     GRGAATFLSKLTTGLAIAFMVISLLIGLISAPRGEPTSIIRQEANRHTSPSAELPIPQ
                     GSSEQGTPVLPGN"
     gene            complement(6160..6939)
                     /gene="tpiA"
                     /locus_tag="PLI55_04425"
     CDS             complement(6160..6939)
                     /gene="tpiA"
                     /locus_tag="PLI55_04425"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPB00112.1"
                     /translation="MKQYIVAGNWKMHKDIQESRIFVSNILNKLLKHERATVILCPPF
                     TALFDVYNLIKNTSIALGGQNMHSEPKGAFTGEISGGMLISAGCQYVILGHSERRHIF
                     GETDDFIRDKVKQALQVGLKPIICVGEKLDERQSGETLEVVRRQCDSALSVVNEQEIK
                     RCVIAYEPVWAIGTGVVATPEQASESHSMIRQLLAAKYGQESAAGTSILYGGSVKSSN
                     AASLIQAQDIDGFLVGGASLIEDEFARIAEIVDEYVKHKES"
     gene            complement(6946..8142)
                     /locus_tag="PLI55_04430"
     CDS             complement(6946..8142)
                     /locus_tag="PLI55_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPB00113.1"
                     /translation="MSRLTVDDLKVKGKRVIMRVDFNVPLDENLNVRDDFRIRAALPT
                     IQKLLNDGARLILMSHLGRPKGKPVPELSLKPVAECLQKYLKNKVYFAPDCVGIEVKN
                     LANSLQDGEVLLLENLRFHPEEEKNDPEFSAKLAELGEIYVNDAFGTSHRAHASTAGI
                     TNYIPVRAAGYLLEKELHYLQDMLENPERPFTAIFGGAKVSDKIDIIERLFGKVDNIL
                     IGGGMAFTFLKAKNYEVGKSLLEADKIDFALATLKKAEQMGCKIVLPTDVVVASEISD
                     TAVPEIVSVDQIPPDKIGVDIGPETTMIFCEIIEKSKTVLWNGPMGVFEVKQFSTGTE
                     SIARKLAEVTTKGVITVVGGGDSASAMKKFGLMDKVSHVSTGGGASLELLSGKELIPI
                     NLISKK"
     gene            complement(8231..8908)
                     /locus_tag="PLI55_04435"
     CDS             complement(8231..8908)
                     /locus_tag="PLI55_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPB00114.1"
                     /translation="MHFFKTLVTGVTSLLFPPICKGCEKRMDSNVGLICADCWQKLQL
                     FPAEAIKNKIIPESLDQVWPVFLFDELFQKIIHALKYQGNVSIGYEIGRRMALHLPSE
                     IKDLQSACYVPIPLHPIKLRERGYNQSEAITRGLASNSGIRVETKLLKRIKNTATQTK
                     LNAEERQANMEMAFAINRKNTVPSDNAIILVDDVFTTGATLNSAAGVLRQSGFHKVIA
                     VTAAAPL"
     gene            complement(8930..9793)
                     /locus_tag="PLI55_04440"
     CDS             complement(8930..9793)
                     /locus_tag="PLI55_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPB00115.1"
                     /translation="MRIGIDLGGTKVSIGLVDQNRLIGQPVRFMIAECKDADDLVKHM
                     KGAIDQSLKANGIVWQNLEMIGIGSPGPLDYKTGIILHTPNLVMLRNYPLGPKLEALT
                     GLPVLVNNDANCFALGEQRAGGGRGLEYVLGVTLGTGFGLGFIYRSEIFNGATGTALE
                     FAMTPYLDGVYEDYISGRGLARIYKSVVGKEIKPAEVTALAKKGDADALETWRIFGHH
                     LAQAMIVLVMVLDPDIIVIGGSVSQGWDFFYQTLETELRAGIHAGPAGHLKITRSELG
                     ELAAIIGAASL"
     gene            complement(9810..11180)
                     /locus_tag="PLI55_04445"
     CDS             complement(9810..11180)
                     /locus_tag="PLI55_04445"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPB00116.1"
                     /translation="MRIKIFLMLMCFLIVNSFAVEFDVDYAVFKGNEEVSVVEVYLLI
                     PRTLFQFVPNENEYRSYALIRVALVEGDSVRDIQKWQITDRVADTLQLAENQKIPEIA
                     TLQVKPGNYQLIIVVADLINRHQYRYDKQIEISDYSDDQLRLSDIQISSQISKTSQEN
                     KFSKYFGYDVIPNASAIFSQRQNLIYSFVEVYNLKVDPGKPSNYLVKYDIADLNGKVV
                     VSSDWRSRKKPGTSAVEINNLSIAGLPGGLYDLIIEVNDEATQKTVKKTKRFYILESK
                     SQLASLEMNAEAIQLEGKSEKELDDIFGPLRYIATETEIRRYKKSDVEGKKQIILHFW
                     DNRDPNPQTPINENKAEFEQRLQYVNQQFSTNQVPGWKSDFGRVFMIYGMPSEIERFP
                     SALESKPYQIWYYNEIEGGVKFYFVDKNGFGTYELVHSTARNELQDINWQRWISPYST
                     SPDLTY"
     gene            complement(11184..14705)
                     /gene="smc"
                     /locus_tag="PLI55_04450"
     CDS             complement(11184..14705)
                     /gene="smc"
                     /locus_tag="PLI55_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPB00117.1"
                     /translation="MYISKLTLYGFKSFLKKSEIQFGRGITSIVGPNGCGKTNIVDAI
                     RWVIGEQKASILRAERVTDVIFNGTATRRPLNLAEVSLTINNVSGRVPIAYSDVEITR
                     RLYRNGESEYFINRNLCRLKDITDLFIDTGMGANAYSIIELKMIENILSETPEERKRL
                     FEEAAGVNKYRVQRKAALRKLESTRDDLLRLNDIINEVDQNVKNLKRQIARYDKYQET
                     TQKLIEAEVILASRRIFDLRAKQAPILTDINAKQNLLEKISTELNAKESQEREQQKIL
                     NTIEIELQNKRNELETLRANKAKLEATGLLLKEQLRNNTQNSARLTATINALQKSIES
                     AETRQKIIEQDLATNAQTLEEKRQAYKAAVEAQQEIENNYKKLSADLQALQDERFNAF
                     RQQAEQTAHLNNLKENINQREKELQKVIEQIATQTDSQARLAIALKELREKIDVLTVQ
                     RDELEKSLQNELSHQQSLTEQENRIRDELRHCESAFDKLNNQIQFYRSIIHSKEGFAP
                     ALQFVLDHPDEFPGIRGALSDLLSVDHQYYLAVEAVIKDIARLLVAENRSAALQTLEK
                     LNRLGKGRISIIPLDARWRTPALKSARNGLQPLLKFIKSDAQLENLKEMLFGNVYCCS
                     DDQFEELINNPELESYSIVTDRGRFRDANGWFSGGAEQSAANVLVGRQARLNELQEEI
                     KSVEKQRTALLEQLQSAQAEIRRHSEMREKLYRDRQQIETEIRQLTDVLRATESQQVQ
                     SAGALAALNDQKVMLTTAIANFKERLAKETPTQTNVDSQLAQMDQLISIKKTELDQVR
                     ANLDGHNQNLQNLRVELIGLENKARNLSDEKVSLERNLKNNLEQITKARQELAALELE
                     KSQLEKNLAENKLADAEAVTLVNQTESNYNQIGQEFQALRQTIEAINTEIMNLRHTKE
                     QNSEELKRLELASSQFIANINEISSVLWEKYNRTPLEEIPPDLPTETEARQVVERYRH
                     NLEQIGMVNMAVKEEYEQEYTRWKFLSDQRDDLVSSEKGLTEVITQIDQVAREQYLET
                     FNQIRSNFKTTFDIFFGGGEADLRLIGSDDPLEAEIDIWACPGGKKMRSLRMLSAGEK
                     ALTAIALLFAIYQVKPSPFCILDEVDAPLDDENTRRFTSVIRTFSENTQFIIVTHNKA
                     TMAIADALYGVTMAEKGVSQIVSVKLE"
     gene            complement(14718..15065)
                     /locus_tag="PLI55_04455"
     CDS             complement(14718..15065)
                     /locus_tag="PLI55_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018194996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPB00118.1"
                     /translation="METDCIFCKIAAGKLGTEFLYESDEIVAFRDIHPKAPTHILIIP
                     RRHIPKITDLQESDEKLVGRMVTVANILAEKEGLKESGFRLIFNCGRDSGQEVFHIHL
                     HLLGGRPFHWPPG"
     gene            complement(15055..15792)
                     /locus_tag="PLI55_04460"
     CDS             complement(15055..15792)
                     /locus_tag="PLI55_04460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPB00119.1"
                     /translation="MHAYQFYISMEKIQEGTFDLEGDEYRHCCRVLRKIRGDSIDVFA
                     GNGRRWSARIEKISNNKAECRIMEEYPPQSRLKPEIILGVGLLKNNLMDDVVVTATAL
                     GVAEIMPLYTTHSIKNKINHERLAKLALSALKQSGLAFLPIISDCLTLDKWFNLTEEI
                     PLKLIAEQHASANLNRIQQLQTVEQVALMIGPEGGFEENEIEEAQRFGFNKVNIFPYR
                     LRTELAVVTALAGIRTLTLKNEAYNGN"
     gene            complement(15792..16481)
                     /locus_tag="PLI55_04465"
     CDS             complement(15792..16481)
                     /locus_tag="PLI55_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00120.1"
                     /translation="MNTISKYKVGLILLGLVMTIPLSAAKKINFTEAKARYSGLTIFQ
                     TNEGSGIGGYFEYSLKNANRLAAQVMFTVATGDDYPLYYYDYYYDQYYYYESASKRRL
                     SFLHLLGGYKRILFADKIANDFRPYLECLGGAVMALDPPNIPDFSDRIKKMTTAWAPG
                     IQIGGGIDFLSGPKTLVSIFAGYDYLKFAQKIDPPQVYYDEDGNPLENRYSGQKDFSG
                     LVIKISFGKKL"
     gene            complement(16649..17686)
                     /gene="asnA"
                     /locus_tag="PLI55_04470"
     CDS             complement(16649..17686)
                     /gene="asnA"
                     /locus_tag="PLI55_04470"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010528228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HPB00121.1"
                     /translation="MNSGLFIPKNYRTILTTVETEKAILALKDFFQQNLAFELDLIRV
                     TAPLFVKAGTGINDDLNGIEQPVSFTVKEMANTQVEIVQSLAKWKRMMLTDLGIQPGK
                     GLYTDMNAIRPDDILDNLHSIYVDQWDWEKAITPEQRNLDFLKKTVSTIYDVILRTEK
                     FVCHEWNISPELPEKITFIHSEDLLDQYPDLPPRAREKEACKKFGVIFLIGIGGQLQN
                     GEPHDGRAPDYDDWSTPTVNGYHGLNGDIIVYNRVLDCAFELSSMGIRVDREALLRQL
                     KIRQVEERQNLYFHQRLLKGELPLSIGGGIGQSRLCMFYLRKAHIGEIQCSIWPEEMV
                     KLCQRNNIFLL"
     gene            17894..18973
                     /locus_tag="PLI55_04475"
     CDS             17894..18973
                     /locus_tag="PLI55_04475"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPB00122.1"
                     /translation="MPVNQIQLMRFKSDEPVDQTKNRLKKLFKSAGLDRVIQAGDITA
                     IKIHFGEKGNVTHVPAAYVEPIVRMIKSCGARTFLTDTTVLYKSVRQNAIDHLQLAYD
                     NGFTYEKVGAPVIIADGIIGRNETEIEVSAPLNKTVSLATELVAANSIIAITHVTGHL
                     STGLGATIKNLGMGMSSRRGKLAQHSVSKPIIKSNKCTACGICMKWCPANAIAIPDKF
                     AIIDQDICIGCGECLTVCRFDAVHFKWNNSSTRLQQQIAEHALGIVRQKSGRIGYLTF
                     LTNMTKDCDCLPGKQVPIAPDIGVLAGFDPVAIDQAVIDLTNQVNNQNIAQLAHPEID
                     YSIQLDYGEKIGLGCRKYQLIETEF"
     gene            19034..20011
                     /locus_tag="PLI55_04480"
     CDS             19034..20011
                     /locus_tag="PLI55_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPB00123.1"
                     /translation="MIDVFFYEAFQEEAELIERFLPENITAGFDWRTIQESEHPAPPA
                     KIISIRTQSIVPVSWLPELSAILSRSTGYDHLIPLAQKAGSRLQVGYLPLYCNRSVAE
                     QAILLALALLRKLPQQLQQFQKFHRDGLTGRECAGKTLLVVGVGNIGHEIVKIGIGLG
                     MQVLGVDIVKKHSDVNYISIEQGLPKADIIICAMNLTSENHNYFNADRLRQAKPGVVF
                     VNIARGEQSPSTELLKLIQEGHLGGVGLDVYNVESELAVALRSGSTSPNKEVQATLAL
                     AKLPNVILTPHNAFNTYESVERKAAQSITQIQHLLENGRFLWPIPADGK"
     gene            complement(20262..21869)
                     /locus_tag="PLI55_04485"
     CDS             complement(20262..21869)
                     /locus_tag="PLI55_04485"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPB00124.1"
                     /translation="MKQSVVCKILVIVVWISFIPLVGIAREKADWEGGYPDGCTSVMV
                     GKKASRDGSVMTSHTCDSHRTRSSFDIVPARKYSKGEMVTMLKRIDCDSLAMPAYQYL
                     AIGEIPQVVSTYGFINTAYPCMNEKQLAIGESTFGGRECLRSDNGLIDCQQLVRLMVE
                     RCSTAREAIRMAGELTKKYGWIDEGECLTIADKNEVWALEIVGPGKGNCGSIWVAQRV
                     PDDHVSVNANGSRIRQIDLKKPDYFMASENVFKVAQDSGWWNPKEGPFEFAYAYAPES
                     RQTFSARRREWRVLDLVAPSLKLHPDAENFPFSVKPDTLVDLAKMIRIFQDYFEGTDY
                     NFVKNITWTNSEGKTEISPFANPFMPYDMNHIFKINGGWGWRGERPIARWYTMYATIT
                     QSREWLPDEIGGVVWLALDNVATSIYIPLYCSIYDVAPSYKVDGRTKGFNRESAWWAF
                     NRLGTLTAQRWGDMRYDVAAVWGPMQSELLRRQNEIESQALRQYRHSPEAARHFLTKY
                     SIDWGAKVVNRAWKLGDELWTKYDEQF"
BASE COUNT         6057 a         5102 c         4363 g         6373 t
ORIGIN      
        1 acagcctttc caggctgttc agtgttttaa attttaatca atatatcgcc ggatttcatc
       61 cagcggtcgg cgctttttcg ggcggtcgga atccgccgga taaccgagac taaacattat
      121 atcaatttta gtagatttgg gaagatcaag agctttacgt acaccatttt cactgaacca
      181 acccagccag caagttccga gccccagttc agccgcttgc aaggcgagat gttcaccagc
      241 aataccaata tccaccaaag catatttgag atggcgaaaa aatccaccaa ctttaacctg
      301 ataagattgt tgttcggtaa taactacaat tatcaccggt gcatttttca caaatttatt
      361 cgaagaataa atcccgctaa aagcggcgct acacaaccgg tctttcactt cctcattatc
      421 aattacaata aagtaccagg gttgagcgtt gcaagccgaa ggtgctaaac gcgcggcatc
      481 caaacatttg tcaatcagtt cacgctctac cggtttcggc aaatatttac gaacactgcg
      541 cctctttaat attaattctg aaaaatccat cttcactcca ttaattcgcg cgcctgtttt
      601 aaagtaattt cgctgactga acgaccgcca atcaatttag cgatttcggc tactctttcc
      661 tcgggaagta atttacgaat ttgagtctga ctgcggccgt tcgtagtaac tttctccacg
      721 ctaaaatgtt cgtcagccag accggcaatc tgaggcaaat gggttataca aatgacctga
      781 tgaaccttgg caagttcatg taactcctca ccgacttttc gcgcaatttt ccccgagata
      841 ccggtatcta tttcatcgaa aatcaacacc ggaatatgat cgcgtccagc caaaatcgtc
      901 ttgatagcca gcataatgcg agaaacttcg ccgccggaaa caatttctgc cagcggtcgg
      961 cgcggttcac cgcgattagt acttatttca aaggttactt tatcaatgcc cgtcgattcc
     1021 gctttgtatt tctgaccatc aattataatc cagccgtttt ggtccggcat caattccagg
     1081 cgtacggcga agtcggctcc gctgattccg agacgttcca gaattttttc tatttcgatt
     1141 ttaaataatt cggcactttg tttacgtttt tcggataatt caaaggcaaa tcgagtgaat
     1201 ttttccagat gttctttaat aagttccctg tttttgtcaa tttgaacctc gagatcatcg
     1261 ctttcggtca gatcggtctc tagcttttgc cgataggcga cgacatcgct cagtgatggt
     1321 ccatactttt tcatcaattg ttgaagacta tacaaacgct gattgatgat ctctaaccgc
     1381 gcgggattaa attgcagatc atcgccaaaa tgggcgattt gccgggcgat ttcctggaat
     1441 agatattgac tctcctccaa tcgggaaagt tgctctacaa atgagccggt aattttttgc
     1501 aaattggcaa gttgcttcac aacgccctgt aattggttat aaagtgagtc gcctgactcc
     1561 agtaattgat aagtcagatc ggaactaagt tgctggattt tagcggtatt ttcgagtata
     1621 gttttttcct gaaggagggt ctcatattca ttttcctgag gagcaacctt ttggatttcc
     1681 tgcaattgaa attcccagag ttcgcgcttc tcgcgattga gtcgtaattg ttcctggagt
     1741 aaattttggc gctcaaaaag tagtttgagc tcatcaaatt gcctcgcaac ctgttcgcgc
     1801 cattcggcgt aatccccaaa tgcatccaaa aattctatat gggtatccgg attcaaaagc
     1861 gactggtgat catgctgacc gtgcatatca accagcaaat tgccaacctc tttaagaaca
     1921 ctcaccaggc aggtttcgcc attaagccag gcacgactgc gaccgttaat atttacctcg
     1981 cgtttcaagg tcaaaggatt attttcctgg taaaaattat gagatttcag aatattttta
     2041 gtggctggat tcaaatcggt gagatcaaag gtgcattcta ctgttgcgac gtctgcaccg
     2101 gctcgtataa cattcggact ggctttttcc cctaacgcta gattcaaggc atcaataata
     2161 attgacttac cggcaccggt ttctcctgtt agaatcgtca atcccggccc gaaagcaact
     2221 tcaatatgat caatgatcgc aaaattatca atttttaatt tagtcagact catgcttctc
     2281 taaatttctg aaagacattc aagcggttga aaatccatac gcccagtaaa attcccagcg
     2341 catcggctcc aagatcagct agtgaactgg tgcgaccggg aacaaagtac tggtgaattt
     2401 catcggttaa gccatataaa acacccagaa taatgctctg gaagttagcg cgccgcatcg
     2461 gtttaccaat ctgatcgctt ctaaaagcca acactagtaa aatcccaaat attgtatatt
     2521 ccagcatatg aaccagctta tcaacgttcc agattggaat ctcaatcggc ggagtttttg
     2581 ttaaagatga taaactaaaa ataaggacgg catataaaat tgccgggact ttcagccaga
     2641 agcgattcat taaatcaccc tatccagccg acgcaatatc agcggtaaat aatccagcaa
     2701 atcactggcg ataaatgccc tgactccgag cttcttacgt ccccaatcac cggctaatcc
     2761 atgaatggaa gttcctgcca tagccgcatc gaaagaattt aagccctgag cccgaaaagc
     2821 cgcaattatg ccggttagaa catcccccgt gccgccagtc gccaaagccg gattgccggt
     2881 cgaattgacc gccaccactc ccgtagaaga aacaaccaca gacggtgcgc cctttaacac
     2941 caaagtacaa ggatactttc gtacgaaatc gcgagcaact tcaatcacat ttttattcac
     3001 ttctgcgact gcaatccccg tcatcattgc aaattcgcca cgatgaggag ttatagttaa
     3061 atcctcaatc gtaccaatta attctaaatt attatggaaa atacgcagac catcagcatc
     3121 aattaccaat ggtttatgac attttgttat caactgtcgc ccgaattcct gaacttcgaa
     3181 actatcggaa actcccggac caaagacaac cacatcactc cattccagtc tggattggag
     3241 tgccgtcaaa gattccagac aaaaagtctg ctgagacgat tccggaagtg gtgcggttag
     3301 cgcctcggtc agtttgactt ccattatcgg attcagactc ttgggaatgg cattcaccac
     3361 cagtccggcg ccactacgca tagcgcctaa tgcgcaaaga gtcgccgctc cggtcattcc
     3421 gggcgaacca gcaattatca aaacttttcc ctgacgatgt ttgtaagtat cttcggcaat
     3481 cggtggaata accttcttga catcctgttt accaattagg aaagtttttt cttttaaaat
     3541 gtcaaaagct ttacgcggat aaccgagatc tactacgaca atttcaccgg cgaatctttt
     3601 gcccggataa aaaagcaaac cacgttttaa aaaacccatc gtcacggtat aatcggcttt
     3661 gacggcatct cctctgactt ctcccgtatc tccatttacc ccagagggaa tatccactgc
     3721 aattaccggt aaattgatat tattaatacg acgtatcaca tcggctataa tcccctgaac
     3781 ttcaccacgg aagccagttc cgagcatcgc atcaattatc aaatcggatt ccggcaagtc
     3841 ggaatattct tcaaagtctt ccgctgtttt tatacaacgg atagtctgct tgctctccac
     3901 ccatttctga taaaaatgac gtgcatcgcc tttgatattt tgcggatcac caatcagcca
     3961 aataatgcaa ttagcgcccc attcacttaa taacaaagcc gcgacaaacc cgtctccgcc
     4021 gttgtttcca attccgcaga aaagatcaat gcgactgttg ggtatgtcct gaatcaattt
     4081 cttggctttc agggcaacaa aattgccggc tttgaacatt aattcagtgc cgggaatttg
     4141 caactcctca attgcatagc gatcaatcgc tgaggcttca gcatttgaaa ggataaattc
     4201 aggcatagta gtcggttctt ttactaatga ttgttttcat atttcgcttt taaaattact
     4261 caaaaacctc tttaagccat agaatttttc ataaaagagt tatccgaata aaaaagggtg
     4321 agactgactc acccttaatt attctgacta gaaaatttta ttgaaccgtt ttcagatgtt
     4381 cttctgcgta gcgccgatat tggggatctt tggcggctaa ttgaaaagcc tgacgagccg
     4441 cggctgtatt tccgaggcaa cgttccgcaa ttcccagttc gattagagcc agagcatcac
     4501 cggctttaat tttcaaagcg gtttcagccg ccgtcttagc ctgagcacat tccccattat
     4561 tattataagc ttgggcaagt ctcaaccaag ccttcacgtt ctttggatct ttttgagtga
     4621 ccttttccaa attgataatc gcttccttgg gattattggt atccagataa atctgcccca
     4681 gtttttcata gtagcgtgga ttctcgggat tgatttcaat caatttttta taggcattaa
     4741 tggactgctc ataatttttc atattgtaat tcaatacccc gattcgatcc caaccggctt
     4801 caaaaagtgg atcaatttta actgcttgtt cgtaagcttt tagggcttca gcctttttgt
     4861 tggtcttctc gtagatttga ccaagcaata agtaggactt ggcatgttcg ggattgattt
     4921 ccaagccttt attgatagct ttgatggctt catcataatt cttcatgtca taataagcca
     4981 gcccactgaa ataataagcc aaaaactcgt tcggatcgag ttcgagtgct tgtttgtatt
     5041 tctccagcgc tccttcatta ttacgtctct ggcgcaggtt ttcaccttcc tgtaagtact
     5101 gctgaaccaa gttctttaat agttttgtat accgttcttc gttcggtttc atttcgataa
     5161 cttttctcaa gtacagtgag gcattcgtca aatcatttag acgaatatat acttgagcca
     5221 gattgaaata agcatccgca aaattgggat tagccttaat aatttcgagg tataaatttt
     5281 tagcgccttc gtaatcacca ttatttacta gtcgagaagc ttctgttagc tgagtagtaa
     5341 aggctgcaat ctgggttcta atctctcgaa actcggtatt ggcacgatct aaatcaatcg
     5401 ctttgtcaag gtaaaccttg gagctatcca gttctccaag tgcaaaataa atccgcccca
     5461 aaaggaaaac agccccagca taattgggat ctttctcaac aataggttta catttttcaa
     5521 atgctttctg atattctccg ttctgaaaga gagtataagc ttctgagaga ggatcttgtc
     5581 caatcaggct actcaacaca accaaagtca ataaaatgat tacactgatt tgttttacat
     5641 atttcatcat ttaccccatt ttattggttt tcattttcgt gccgaggacg ggacttgaac
     5701 ccgtacaagc aaatccgccc acatgcccct caaacatgcg tgtctaccag ttccaccacc
     5761 tcggcaatta ttaactattt caaaaagcta tctggataaa tttgcaatta atttaattac
     5821 caggcaggac gggagttccc tgttcgctac taccttgcgg aatcggcaat tcagctgaag
     5881 gcgaggtatg acgattagcc tcttgacgga taatactggt gggttcgccg cgcggagccg
     5941 aaattaatcc aattaaaaga gaaataacca taaaggcaat cgccaaaccg gtcgttaact
     6001 tcgaaagaaa agtagccgcg ccacgtcccc cgaataaaga actgcctccg gcgccaccaa
     6061 acgtaccgga caatccgcct cccttgctgg attgcattaa aattaccagc attaataagg
     6121 ctgacacaat tacatgaatt attattaaga aggtatacac tatgactcct tatgttttac
     6181 gtattcgtca acgatttcgg caatccgggc gaattcgtct tcaattagac tggcgccacc
     6241 aaccagaaat ccatcaatat cttgcgcttg aatcaaactc gcggcattcg aagatttaac
     6301 gctaccgccg tacagaatcg aagtcccagc cgcgctctcc tgcccatatt ttgcggctaa
     6361 aagttgccga atcatagaat gagattccga agcttgctcg ggtgtagcga caacaccggt
     6421 tccaatcgcc cagaccggtt cataagcaat cacacatcgc tttatttcct gttcattgac
     6481 gacgctcaag gctgaatcac attgtcggcg gacaacctcg agggtttcgc cggattgacg
     6541 ctcgtccaat ttctcgccaa cgcagataat cggtttcaaa ccaacctgca aagcttgttt
     6601 gactttatcc cgaataaagt catccgtttc gccgaaaata tgtcgccgct ccgaatgccc
     6661 taaaatgaca tattgacagc cggccgaaat cagcatccca ccggaaattt cgccagtaaa
     6721 tgcacccttg ggttcgctgt gcatattttg gccgcccagc gcgatggaag tgttttttat
     6781 taagttataa acatcaaaaa gcgccgtaaa aggaggacat aaaataaccg ttgctctttc
     6841 gtgtttcagc aacttattga gaatgttcga cacgaaaatg cgagactcct gaatgtcctt
     6901 atgcatcttc cagttaccgg ctacaatata ttgtttcata atcaactact tctttgatat
     6961 aagattaatc ggaattagtt cctttccgct caacaattcc agcgaagcac caccgcctgt
     7021 cgaaacgtgg gaaaccttgt ccattagtcc gaattttttc atcgcggaag ccgaatcgcc
     7081 accaccgacg acggtaataa cgcctttagt cgttacttcc gccaatttgc gcgcgattga
     7141 ttctgtaccg gtagaaaatt gtttgacttc gaagacgccc atcgggccat tccacaggac
     7201 tgttttgctt ttttcaatta tctcgcagaa aatcatcgtg gtctcaggac cgatatcgac
     7261 accaatttta tctggtggaa tttgatccac ggagacgatt tccggcaccg ccgtatcact
     7321 gatttcagat gcaacgacta catcggtcgg caaaactatt ttacatccca tctgttcggc
     7381 tttcttcaaa gtcgccaatg caaaatcgat tttatcagcc tccagcaatg atttaccgac
     7441 ttcataattt ttggctttta gaaaggtaaa tgccatcccg ccaccaatca aaatattatc
     7501 aactttacca aacaaacgct cgataatatc aattttatca ctgactttcg ctccgccgaa
     7561 aatagccgtg aacggtctct ccggattctc aagcatatcc tgcaaataat gcaactcctt
     7621 ttcaagcaaa taaccagccg cccgcactgg gatatagttc gtgataccgg cggtggaggc
     7681 atgagctcga tgacttgtac cgaaagcatc gttgacgtaa atctcgccca actcagctaa
     7741 cttggcagaa aattcgggat catttttctc ctcttccgga tgaaaacgca gattctccag
     7801 cagaagaact tcaccatcct gaagcgaatt ggctaagttt ttcacttcga tcccgacaca
     7861 atcaggcgca aaatatactt tgttcttcaa atatttctga agacattcgg cgaccggctt
     7921 aagactcaat tccggcacgg gtttaccttt tggacgaccg agatgcgaca tcaatatcaa
     7981 acgggcgccg tcattcagta acttttgaat agtcggtaaa gccgcccgaa tgcgaaaatc
     8041 atctcgaaca ttcaaatttt catcaagcgg gacattgaaa tcaacccgca taatgactct
     8101 tttacccttt acttttaaat catcaacagt caatcttgac atatttcacc tttagtttat
     8161 tcattgttta aaataacttt caattttaat ttcttagagc aattattcca aggaaaaata
     8221 ttgattcctg ctaaagtgga gcggcggcag ttaccgctat aactttatga aaaccggact
     8281 ggcgtagtac tcccgcggct gaatttagag tagcaccggt tgtgaaaacg tcatcaacca
     8341 gaataatagc attatcggaa ggaacagtat ttttccgatt aattgcgaag gccatttcca
     8401 tattcgcctg gcgttcctca gcatttaatt tggtttgagt tgccgtattt ttgattcgct
     8461 taagtaattt ggtttcaact ctaatgcctg agttcgacgc caatccgcgt gtaatcgctt
     8521 cggattgatt atatccgcgt tcccgcaatt ttatcgggtg caaaggaatc ggaacgtaac
     8581 aagccgattg gaggtctttt atttcagatg gcaaatgaag agccattcgt cgtccaatct
     8641 cgtaaccaat cgaaacattt ccctgatatt tcaaagcgtg aattattttc tgaaacaatt
     8701 catcaaatag aaaaaccggc cagacctgat caagactttc cggaataatt ttatttttta
     8761 tcgcttccgc cggaaataat tgtaattttt gccagcaatc agcacaaatc agccctacat
     8821 ttgaatccat ccttttttca caacctttgc agatcggtgg aaatagcaga gaagtaacac
     8881 cagtcacgag agttttgaaa aagtgcataa ttaaaacgat cagctttttt taaagactag
     8941 ccgcgccgat aatagccgcc agttcgccta attcactgcg ggtaattttc agatgaccgg
     9001 ctggaccggc atgaattcct gcccgcagtt cggtttccag cgtttgatag aaaaaatccc
     9061 aaccttggga cactgatccg ccaattacga taatatccgg atcgagcacc ataaccagaa
     9121 ctatcatcgc ttgggcaaga tgatggccga atattcgcca ggtttccaga gcatctgcat
     9181 cacctttttt agctaaagcg gtaacctcag ccggtttgat ttcctttcca accacagatt
     9241 tatagatgcg cgccaagcct cggccagaaa tataatcctc ataaacacca tcaagataag
     9301 gagtcatcgc aaactctaaa gccgttccgg tagctccatt gaaaatttcg ctacgataaa
     9361 tgaatcccaa cccaaatccg gtacccagag ttacaccgag aacatattcg agaccacggc
     9421 cacctccggc gcgttgttct ccgagcgcaa aacagttggc atcattattc accagcaccg
     9481 gtaaaccggt caaagcctcc agctttggtc cgagtggata attgcgcaac atcaccagat
     9541 taggagtgtg taaaattatc ccagttttat aatcgagcgg tccgggacta ccgattccga
     9601 tcatttccag attctgccag acgatcccgt tagctttaag actttggtca atagcgcctt
     9661 tcatatgttt aactaaatcg tcggcgtctt tacattcggc tatcataaaa cgaaccggtt
     9721 gaccaataag ccgattttga tctaccaatc caatcgaaac ttttgtgccg ccgaggtcta
     9781 ttccaattct catcctttta cctaccttcc taataagtta aatctgggct ggtggaataa
     9841 ggcgatatcc agcgttgcca gtttatatcc tgcaattcat tgcgagcagt cgaatgaacc
     9901 agttcgtatg taccaaatcc atttttatca acgaaataaa atttcacgcc gccttcgatt
     9961 tcattataat accagatctg atagggtttg ctctcaagcg cactgggaaa gcgttcaatt
    10021 tcggagggca ttccgtaaat cataaaaacc cgtccaaaat ccgatttcca accggggact
    10081 tgattggtag aaaattgctg attgacatat tggagacgct gttcgaattc ggctttattt
    10141 tcattaattg gtgtttgggg attaggatcg cgattatccc agaaatggag gataatttgc
    10201 tttttccctt caacatcgga ttttttgtag cgtctaattt ccgtttcggt ggctatgtat
    10261 cgtaaggggc caaaaatgtc atcaagttct ttttcagatt tgccctccag ctggatagcc
    10321 tcggcattca tttcgagact ggcaagctga gatttgcttt cgagaatgta aaaccgtttg
    10381 gtcttcttca ctgtcttttg agtcgcctca tcatttactt caataattaa atcatacaaa
    10441 ccacccggta agccagcaat actgagatta ttgatttcga ccgccgaagt gcccggcttc
    10501 tttcgagaac gccaatcact acttactact acttttccat ttaagtccgc aatgtcatac
    10561 tttactagat aattcgacgg tttaccggga tcaactttca agttataaac ttcgacaaaa
    10621 gaataaatca aattttgcct ttggctaaaa atggcactgg cattcggaat cacatcataa
    10681 ccgaaatatt tactgaattt gttttcttga ctggttttag aaatctggct actaatctga
    10741 atatcactga gccttaattg atcatccgaa taatctgaaa tctcgatttg cttatcataa
    10801 cgatactgat gtcggttaat cagatccgct acaactatga tcaactgata attgccgggt
    10861 ttgacctgca aagtagctat ttccggaatt ttctgatttt cagctagttg taatgtatca
    10921 gcaacgcgat cagtaatctg ccacttttga atatcgcgca cggaatctcc ctctaccaga
    10981 gcaactcgaa taagagcata ggaacgatat tcattttcat taggaacaaa ttgaaataac
    11041 gtccgcggaa tcaggagata gacttcgacc acactgactt cctcattgcc tttgaagacc
    11101 gcataatcta catcgaattc tacggcaaat gagttcacaa tcaagaaaca cattaacatt
    11161 aaaaatattt tgatacgcat aggttactcc aatttaaccg aaacgatttg tgagacacct
    11221 ttttcagcca tcgtgactcc gtataaagcg tcggcgatcg ccatcgtcgc cttattatga
    11281 gtaacgatta taaactgggt attttccgaa aaagttcgaa tgacgctggt aaagcgacgc
    11341 gtattttcat cgtcaagagg cgcatcaact tcatccagaa tacaaaaagg actgggtttg
    11401 acctgataaa ttgcaaatag taaagctata gccgtgaggg ctttctcacc ggcagaaagc
    11461 atccgcagtg agcgcatttt cttaccaccc gggcaagccc agatatcaat ctcggcttcg
    11521 aggggatcat ccgaaccaat tagacgcaga tcggcttcgc cgccgccgaa gaaaatgtcg
    11581 aaagtggttt taaaattgct ccggatttga ttaaaagttt ccaaatactg ttcacgggcg
    11641 acctggtcaa tctgcgtaat cacttctgtc aatccttttt ccgaagatac taggtcatca
    11701 cgctgatcgc ttagaaattt ccagcgtgtg tattcctgtt catactcttc cttgactgcc
    11761 atatttacca tgccgatttg ctccaaattg tgtcggtagc gttcgacaac ctggcgggct
    11821 tccgtttcag tgggcaaatc gggcggtatt tcttctaatg gcgtccgatt gtatttttcc
    11881 cagaggacgg agctgatttc attgatatta gcaataaact gagaactagc cagttccaga
    11941 cgtttcagct cctcactgtt ttgttctttg gtatgacgca aattcataat ttcggtatta
    12001 atcgcttcaa tcgtctgccg tagtgcctga aactcttgtc caatctggtt gtaattagat
    12061 tctgtttgat tcaccagagt tactgcctcg gcatcggcga gtttattttc tgccaaattc
    12121 ttttccagtt gactcttttc caattctaaa gcggcgagtt cctgccgggc ttttgtgatc
    12181 tgctcaagat tatttttcaa gtttcgttct aaagatacct tttcgtcaga taaattgcgg
    12241 gctttatttt cgagaccaat tagctcaact cgcaggtttt gcaaattttg attatgccca
    12301 tcgaggttag cccgtacctg gtcgagttcg gtctttttaa tagatataag ttgatccatc
    12361 tgggctaatt gactgtcaac attagtttgg gtgggagttt ctttagccag cctttctttg
    12421 aaattggcaa tcgcggtggt aagcatcact ttttggtcat taagagccgc cagagcccca
    12481 gccgactgca cttgttgtga ttccgtagcc cgcaagacat cggtcaattg gcgaatttca
    12541 gtttctatct gctgacgatc gcgatacagt ttttcacgca tttcactgtg gcgacgaatt
    12601 tcagcctgag ctgattggag ctgttcgagc agagcggttc tttgtttttc aaccgattta
    12661 atttcttctt gtaattcatt cagtcgggct tgacgaccga ccagcacatt ggcggctgat
    12721 tgttcggcgc cgccggagaa ccagccattc gcatcgcgaa aacgtccgcg gtcagtaaca
    12781 atcgaatatg actccagctc tgggttattg attagttctt caaattgatc atctgagcaa
    12841 caatagacat tgccaaaaag catctctttc agattctcca gctgagcatc cgatttaata
    12901 aattttagaa gtggctgtaa accatttctg gccgatttaa gggcaggcgt acgccatctg
    12961 gcatctaatg gaattattga tattcgtcct ttcccaagac gatttaattt ttcaagggtt
    13021 tgcaaagccg ctgaacggtt ttcggcaact agtaatcggg cgatatcctt gattacggct
    13081 tcaacggcta aataatattg atgatcaacg gataataaat cggataaagc gccacgaatt
    13141 cccggaaatt catcaggatg atcgagcacg aattgtaagg ctggcgcaaa cccttcttta
    13201 gagtggataa ttgagcggta aaattggatc tgattgttca atttatcgaa cgccgattca
    13261 caatgacgca attcatcccg aatgcgattt tcctgttctg ttaaagactg ctgatgagat
    13321 aactcgtttt ggagagattt ttccagctcg tcgcgttgaa cggtcagcac atctattttt
    13381 tcacgaagtt ccttaagtgc gattgccaaa cgagcctggc tgtcagtttg agtagcaatt
    13441 tgttcgataa ctttttgtaa ttctttttcg cgctgattga tattttcttt aagattattt
    13501 agatgggcag tttgctcggc ttgttgccgg aaggcattaa agcgctcgtc ctgaagtgcc
    13561 tgcaaatccg cacttaattt tttataattg ttttcgattt cctgctgggc ttcgactgcc
    13621 gccttgtaag cctgacgctt ttcttcaagg gtttgggcgt tggtagccaa atcctgttcg
    13681 attattttct ggcgggtttc cgctgattcg atacttttct gtaaagcatt aatcgtggcg
    13741 gttaggcgag cgctgttttg agtattgtta cgaagttgct ctttgagaag tagtccggtg
    13801 gcttccaatt tggctttatt agcccgcaaa gtctcgagtt cattacgctt gttctgtaat
    13861 tcgatttcga ttgtatttag aattttttgt tgttcgcgtt cttgtgattc tttggcattc
    13921 agttcggtgc taattttttc cagtaaattt tgttttgcgt taatatcggt taaaatcggc
    13981 gcctgttttg cgcgtaggtc gaagatgcgg cgactggcta agattacttc ggcttcgatt
    14041 aatttctggg tggtttcctg atatttatca tagcgcgcaa tttgcctttt caaattttta
    14101 acattttgat caacttcatt gataatatca ttcagacgga gtaagtcgtc gcgagttgat
    14161 tcgagtttac gcaaggcggc tttacgctgg acgcggtatt tattgacacc ggcggcttcc
    14221 tcgaagagac gcttgcgctc ttccggcgtt tcactgagaa tattctcgat cattttgagt
    14281 tcgattattg aataggcatt cgcccccata ccggtatcaa taaatagatc ggtaatgtct
    14341 ttcagacgac aaagattgcg attgataaaa tattcacttt caccgttccg gtataatcgc
    14401 cgcgtaattt cgacatcgga atatgcgatc ggtactcgtc cactaacatt attaattgtc
    14461 aaacttactt cggctaaatt cagcggtcgg cgggtagcgg ttccattgaa aattacatcc
    14521 gtgacgcgtt cggcgcgcaa aatgctggct ttctgttcac caataaccca gcggattgca
    14581 tccacgatat tggttttacc gcaaccgttc ggtccaacga ttgaagtaat ccctcgtccg
    14641 aattggattt cggatttttt gagaaatgat ttaaaaccgt aaagtgtgag tttagaaatg
    14701 tacaattacg cttttcctta acccggaggc cagtgaaatg gacgtccgcc cagtaaatgt
    14761 aagtggatat ggaaaacttc ctgaccacta tcacgtccgc aattaaatat cagacggaat
    14821 ccgctttctt tgagcccttc cttttccgcc agaatatttg cgaccgtgac cattcgtccg
    14881 actaactttt catcggattc ctgcaagtca gttatcttgg gaatatggcg gcgcggaatt
    14941 atcaatatgt gggtgggagc tttgggatga atgtctcgaa aagctactat ttcatccgat
    15001 tcatataaaa actccgtgcc gagtttccct gcggcaattt tacagaaaat gcaatcagtt
    15061 tccattataa gcctcatttt ttaacgtcag cgtccggatt ccggcgagag ccgttacgac
    15121 tgctaactcg gtgcgcagac gatacggaaa aatatttact ttattgaaac caaaccgctg
    15181 agcttcttct atttcgtttt cttcgaatcc accttccgga ccaatcatta acgctacttg
    15241 ctcaaccgtt tgtaattgct gaatacgatt caagttggcc gaagcgtgtt gttcggcaat
    15301 cagcttgaga gggatttctt ccgtcagatt aaaccattta tccagcgtca aacaatccga
    15361 aattatgggt aaaaaagcca gtccggattg tttcaaagcc gatagtgcta atttcgccag
    15421 ccgttcgtgg ttgattttat tttttatcga atgagtggta taaagtggca taatctcagc
    15481 gacgcccagc gccgtagcgg taacaaccac atcatccatt aaattatttt tcaataagcc
    15541 aactccgaga atgatttccg gcttcaaccg cgattgaggt ggatactctt ccattatgcg
    15601 acattcagct ttattattgc taatcttttc gattcgtgct gaccagcgac gaccgttgcc
    15661 tgcgaagacg tcaatagaat cgcccctaat tttgcggagg acgcgacaac aatgtcggta
    15721 ttcatcaccc tcgaggtcga atgttccttc ctgaattttc tccattgaaa tatagaactg
    15781 ataagcgtgc attataactt tttaccaaaa ctaattttga tcaccaatcc gctgaaatcc
    15841 ttttgaccgg agtatctgtt ttctaagggg ttaccgtcct cgtcataata gacctgcggt
    15901 ggatctattt tttgagcgaa cttaagataa tcgtaaccgg cgaagatcga aacgagcgtt
    15961 ttaggcccgg aaaggaaatc aatgccacct ccgatctgga tacccggtgc ccaggctgtg
    16021 gtcattttct taatcctatc actaaaatcg ggaatattcg gcggatcgag tgccataacc
    16081 gctccaccca gacattcaag ataaggacgg aaatcattag caattttgtc cgcaaataat
    16141 attcttttat atccgcctaa taaatgcaga aatgataaac ggcgcttgct ggcactttcg
    16201 tagtaataat attgatcata atagtaatca tagtaataga gcggataatc gtcaccggtg
    16261 gcaaccgtaa acatgacctg ggcggctaag cgattggcat ttttaagcga atattcaaaa
    16321 taaccaccga ttcccgaacc ttcgttggtc tggaatatcg tgagaccaga atagcgggct
    16381 ttagcttcgg taaaattaat ttttttggcg gcagagagcg gtatagtcat tactaatcca
    16441 agcaggatta atcccacttt atatttgctt attgtgttca taatgactcc atcatttgtc
    16501 cttcaataaa caactaagaa tttacacttt gagataggaa agcccaagag attgtttttg
    16561 acccgatgcc aataggcgaa aattgagaaa ttccagtatg aaccggttta attaatatca
    16621 aggggcttgg ttgaaaaaat acaaacagct ataacagaaa aatattgttc ctctgacata
    16681 atttgaccat ttcttcaggc cagatactgc attgtatttc cccgatatga gctttccgta
    16741 aataaaacat acataaccga gattgcccga tgccaccgcc gatcgacaaa ggtaattcgc
    16801 cttttaatag acgctgatga aaataaagat tctgacgttc ttcaacctgg cgaattttca
    16861 gctggcgcag aagggcttct ctgtcaaccc ggattcccat cgaagacaat tcaaaagcgc
    16921 aatccaaaac tcggttgtat acgataatat cgccgtttag cccatgataa ccattgacag
    16981 tcggggttga ccaatcatcg taatccggtg cgcgcccgtc atgaggctcg ccattctgta
    17041 actgtccgcc gataccaatc agaaaaatga caccgaattt tttgcaagct tccttttcac
    17101 gggcacgagg tggcaaatcc ggatattggt ccagtaaatc ctcagaatga ataaaggtta
    17161 tcttttccgg caattcaggt gagatattcc attcatgaca aacgaatttt tcggtacgta
    17221 gaatcacgtc gtagatggta ctgacagttt ttttaagaaa atccaagttg cgctgttccg
    17281 gagtgatggc tttttcccaa tcccattgat cgacataaat tgaatgcaaa ttgtctaaaa
    17341 tgtcatcggg acgaatagca ttcatgtcgg tataaagccc tttcccaggt tgaataccaa
    17401 ggtcagtcag catcatccgc ttccatttag ctaaagactg aacgatttcg acttgggtat
    17461 ttgccatctc cttaacggtg aaggatactg gttgctcgat cccattaaga tcatcgttta
    17521 taccggtgcc ggctttgacg aacaacggtg cagttacccg gatcaaatcc agctcaaaag
    17581 ccagattctg ctgaaaaaaa tctttcagcg ctaaaatcgc cttttcggtc tccactgttg
    17641 ttaggattgt ccgataattc ttgggaataa acaatccgga attcattgat tcttctcctg
    17701 aaaaaacgct caactttata aacaatttaa ctattatcca attctttata aaatatttct
    17761 catggcaatt aatccggtaa ttgtcaaata cacggctaca aataattcta cagagattta
    17821 tttcaatttt gagatttttc aaattgccaa ttgaccagcg ggctattaaa ttccgcccaa
    17881 atggagaaaa aatatgcctg ttaatcagat tcagcttatg cgtttcaaat ccgacgagcc
    17941 ggtagatcag acaaaaaatc gtctgaaaaa attgtttaaa agtgccggtc tggatagagt
    18001 aatccaagcg ggcgacatta ccgcgataaa aattcacttc ggtgagaaag gcaacgttac
    18061 tcatgttcca gccgcctatg tcgaaccaat tgtccgaatg ataaagagtt gcggcgctcg
    18121 gacatttttg accgatacca cggtgctgta taaaagtgtg cgtcagaatg ccattgatca
    18181 tcttcagctg gcttacgata acggatttac ctatgaaaaa gtcggggcac cggttattat
    18241 cgccgacgga attatcggcc ggaacgagac cgagatcgag gtcagtgcac ctctgaacaa
    18301 aaccgtgtcg ttagcgaccg aactggtagc ggcaaattct atcattgcca ttactcacgt
    18361 caccggtcat ttgagcaccg gtctgggcgc caccatcaaa aacctcggca tggggatgtc
    18421 cagtcgtaga ggcaaattag ctcaacattc cgtcagtaag cccataatta aatccaataa
    18481 atgcaccgct tgtggcattt gcatgaaatg gtgtccggca aatgctattg cgattcccga
    18541 caaattcgct ataattgatc aagatatttg tatcggttgc ggtgagtgtt tgaccgtctg
    18601 tcggtttgac gctgtccatt ttaaatggaa taatagtagc acgcgtttac aacaacagat
    18661 tgcggaacat gctcttggaa ttgttcggca aaaaagcggt cggattggct acctgacctt
    18721 tctaactaat atgaccaaag actgtgactg cctgcctggg aaacaagtcc cgattgcccc
    18781 cgatattggc gttctggctg gcttcgatcc cgtagcaatt gatcaagccg tcatagatct
    18841 gaccaaccaa gttaataacc agaatattgc ccaattagcc catcctgaaa ttgactatag
    18901 cattcagctc gattacggtg aaaaaatcgg gctgggctgt cgcaaatatc agctaatcga
    18961 aacggaattt tagattccac taaactagtg ttttaaacat cattaaattg attaattagg
    19021 aacagggatt tttatgattg acgtattttt ctacgaagca tttcaagaag aagccgagct
    19081 gatcgagcgc tttctgcctg aaaatataac cgctggattt gattggcgaa ccattcagga
    19141 aagtgagcat cctgcaccac ccgctaaaat aattagtatc cgaacgcaat caattgtgcc
    19201 agtctcctgg ttaccggaat tatcggcaat tttaagccgg agtaccggct acgatcacct
    19261 cataccgtta gctcaaaaag ccggttcaag actacaggtc ggatatttgc cgctttattg
    19321 taaccgttcc gtagcagaac aggctattct cctcgctctg gcgcttctgc gcaaacttcc
    19381 ccagcaactc cagcagtttc aaaaattcca ccgcgacgga ttaactggtc gagaatgcgc
    19441 cgggaaaaca cttctggtcg ttggtgtcgg aaatatcggc catgaaattg taaaaattgg
    19501 cattggatta ggaatgcagg ttctgggagt tgatattgtt aaaaaacact ccgatgttaa
    19561 ttatatcagt atcgagcagg gtttaccgaa agccgatata atcatttgtg cgatgaactt
    19621 gacctccgaa aatcataact atttcaatgc cgaccgactt cgtcaggcta aaccaggtgt
    19681 ggttttcgtc aatatcgctc gtggcgagca gtcaccttca accgaattat taaaacttat
    19741 acaggaaggc catctcggcg gcgtcggtct agatgtttat aatgttgaaa gtgaattggc
    19801 tgtcgctttg cgaagcggaa gtacaagccc taacaaagaa gtccaggcta ctcttgctct
    19861 tgctaaatta ccaaatgtca ttctaactcc tcataacgct ttcaatacct acgaatcagt
    19921 cgaacgcaaa gccgctcaga gtattactca gattcagcat ttgcttgaaa acggccgatt
    19981 tctatggcca atcccggctg atggcaagta gaagaattac cacgaaggca ctaagacact
    20041 aaaaatttac cgtatattta tcgggcttgg taaggagccg aaccgatttg tctattaccc
    20101 tggcaaggtc aagaaaaaag tagcaaacat ttggaaaatt cttttaaact agtcatcctt
    20161 ctggcggcat aacctaagat ttatttgttg acctatcaaa tttcattctt cttaaaataa
    20221 taaaaagaaa tcatagtgcc tttgagcctt tgtggtaaaa attaaaactg ctcatcatac
    20281 ttagtccaga gttcatcacc caatttccag gcgcggttca caactttagc cccccagtca
    20341 atgctatatt tagttagaaa atggcgggcg gcctccgggc tatgtctata ttgcctcaaa
    20401 gcctgacttt cgatttcgtt ctgccgtctt aataattcgg attgcattgg tccccacaca
    20461 gccgctacat catagcgcat atctccccaa cgctgagccg ttagagttcc cagccgatta
    20521 aaagcccacc aggcggattc ccgattaaag cctttcgttc gtccgtcaac cttataggat
    20581 ggcgccacat cataaatact acaatatagc ggaatatata tcgaggtagc gacattatcc
    20641 agagctagcc aaaccactcc gccaatctca tctggaagcc attcccgcga ttgagttatc
    20701 gtagcataca tcgtatacca acgagcgatc gggcgttcac cacgccaacc ccaaccgcca
    20761 ttgattttga aaatgtgatt catatcgtat ggcataaatg gatttgcaaa tggtgagatt
    20821 tcggtctttc cttctgagtt agtccaggtg atatttttaa cgaaattata atccgtaccc
    20881 tcgaagtaat cctggaatat tctaatcatt ttagccaagt ctaccagagt atccggttta
    20941 acagaaaatg gaaaattttc cgcgtctgga tgtagtttta gagatggcgc tactaagtct
    21001 aatacacgcc attcacgcct tcgcgccgaa aacgtttgac ggctttcggg cgcataggca
    21061 taagcaaatt cgaatggacc ttctttcgga ttccaccacc cgctatcttg tgccacctta
    21121 aagacatttt cggaagccat aaaataatcg ggctttttca ggtctatctg acggatgcga
    21181 ctaccattag cattcaccga aacgtgatca tccggaacgc gttgagctac ccagattgaa
    21241 ccacaattac cctttcctgg tccgacgatt tccagtgccc agacttcatt tttatcggca
    21301 attgtcaaac attctccctc atcaatccag ccgtatttct tagtcaattc acccgccata
    21361 cggatcgctt cgcgagctgt cgaacaacgt tctaccatca gacgcaccaa ttgctgacag
    21421 tcaattaagc cgttatctga acgtaaacac tcgcggccac caaaagtaga ttcgccgatt
    21481 gctagctgtt tttcattcat acaagggtag gccgtattga taaatccata agtactgaca
    21541 acttgcggga tttcaccgat cgccagatat tgataggcag gcatcgccaa agaatcacaa
    21601 tcaatccgct ttagcattgt aaccatttca cccttactat attttctggc tggaacaata
    21661 tcgaatgacg aacgtgtgcg atgactatcg caagtgtgac tggtcataac ggaaccatcg
    21721 cgggaggctt ttttcccaac cataactgaa gtacaaccgt ccggatagcc tccttcccag
    21781 tcggcttttt cgcgcgctat cccaactaat ggaataaagc tgatccaaac aactatcacc
    21841 aaaattttac aaacaacaga ttgcttcata ttaatctcct atataatttt gaaaa
//
LOCUS       DAOOMU010000031        21378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2403_length_21378_cov_22.603480, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000031 DAOOMU010000000
VERSION     DAOOMU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 21378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21378
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2403_length_21378_cov_22.603480"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..145
                     /locus_tag="PLI55_04490"
     CDS             <1..145
                     /locus_tag="PLI55_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HPB00125.1"
                     /translation="RKGVCCDCVRYHLRSRQLPACFFPDDVERTYDRSFEKFTELVQR
                     GKV"
     gene            158..823
                     /locus_tag="PLI55_04495"
     CDS             158..823
                     /locus_tag="PLI55_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPB00126.1"
                     /translation="MKRHFYWLKLIICEALMFNLNNLAAEPGEIRQLPKPDAVGRVPI
                     ETTLSQRRSVRTFQNEPLSEKEIAQLLYAAQGISDSSQGLRTAPSAGATYPLEIYLVT
                     AEGIFHYLPERHAIVLKKAGDCRDHLAEASLGQNSVRTAPAIIVFSAVYERTMRRYHK
                     RGERYVHIEVGHAAQNVHLQAVALGLASVPVGAFNDEAVARVIGVERNERVLYLVPVG
                     RKR"
     gene            993..2195
                     /gene="rocD"
                     /locus_tag="PLI55_04500"
     CDS             993..2195
                     /gene="rocD"
                     /locus_tag="PLI55_04500"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPB00127.1"
                     /translation="MLRSQDYIKIEDQYGAHNYQPLDVVLTRGQGIWLEDVEGKKYMD
                     FLAAYSAVNQGHCHPRLVKVMQEQCARLTLTSRAFRNDQWPLLAKELCELTGYEMVLP
                     MNSGAEAVETAIKAARKWGYLYKGVARDKAEIIVCTGNFHGRTTTIISFSTDEQYKDG
                     FGPLTPGFVAIEYGSIDELKKAINPNTVAFLVEPIQGESGVIVPPEGWLKASSEICRK
                     NKVLLIADEIQSGLGRTGKLFACEHENVRPDVVIIGKALSGGMYPVSAVVASQEILGV
                     FKPGDHGSTYGGNPLGSAIAREALKIIIDEKLPQKSAESGAYLMQKLNEIESPYIEQI
                     RGKGLWVGIVLKPSAGGARHFCVALQERGILAKETHENVIRLAPPLNITRSELDHAIA
                     ILQEVLTQ"
     gene            2246..3703
                     /locus_tag="PLI55_04505"
     CDS             2246..3703
                     /locus_tag="PLI55_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPB00128.1"
                     /translation="MKEAIEGLKPASLWRYFSEMSQIPRESKHEEAVIAWLESVAKKL
                     NLPYRKDDVGNIVISKPASPGYENRTPVVIQGHVDMVCEKNSGTVHDFRKDPIELVRD
                     GEYIRANGTTLGADNGIGVAAALAILEDKTLAHPPLEALFTIDEETGLTGANSLKPGF
                     IKGKILLNCDSEEDGALYVGCAGGKQTDLFFDINWEKAPQQMANVALKISGLKGGHSG
                     LDIDQGRANAIKQLNRIVWKAAEKYDFRLYYLEGGSKHNAIPREAVYKLLIKPADLAN
                     FETEIAKFEKILNNEYKDVERGIKLTVENLNEYPKQVFTKEFQARVLNCLYAIPHGVK
                     RMSPTIKGLVQTSNNAAIVSTGDKQIRVLTSQRSSVASEIEGLADKVKACGLLANAEV
                     VQSGGYPSWTPNMDSKILTLMKKVYADLFGVEPQVKAIHAGLECGIIGATSPGMDMIS
                     FGPTIEGAHSPDERVEIKTVEKFWRLLVETLKRIE"
     gene            4135..4944
                     /gene="uppP"
                     /locus_tag="PLI55_04510"
     CDS             4135..4944
                     /gene="uppP"
                     /locus_tag="PLI55_04510"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HPB00129.1"
                     /translation="MNYFESFILGIVQGLTEFLPISSSGHLVIVQHLFGLATDNIAFE
                     VMVHAGTLLSVLAVYYNDVWRMIVSFCRGIFRLRKAQPFQNDSYFRLSIFVIVATIPA
                     VIFGLTLDDFFEQIFHSVFLVGITLTITGLILLSTRWVKTEGRRLTFGKSLLIGCAQA
                     VAILPGISRSGSTISGGLWCGLDRMEATRFSFLLSVPAILGALVLHLKDLIGMALNPN
                     MIGILILGFLTSFIVGYIAIRFLLRVVQSDKFSWFGIYCIIVGLTVVFFLE"
     gene            4983..5954
                     /gene="holA"
                     /locus_tag="PLI55_04515"
     CDS             4983..5954
                     /gene="holA"
                     /locus_tag="PLI55_04515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPB00130.1"
                     /translation="MSKSDFLSELARIEQGHLDAVYLLRGGDLYLEDEAINTICQAFG
                     KQESERPEKLIYYGGVDSDLEFIDNLFSMGFFARRRIIIYKNIGNLEPNLRARLFNFI
                     ENPGQDTVLIMTADGESHSSLIEQLRKESRKVKTISTWTPKPSRFDEFIRRHLQKSQY
                     SIRPEALKLLIELTDDALSHTIGELEKLLIYIGERKMIEEDDVRLIVGGDKNYDMINF
                     LEAVAHRDITKAIKIGLALIQADASIPYFTTQLYDLFSGIWDYEGHGQKEKFWKKSNC
                     YELGIKNYKQADFGAIFTCIRNVDLQSKSLNLNKEDLLIQLLYEIIS"
     gene            5968..6549
                     /locus_tag="PLI55_04520"
     CDS             5968..6549
                     /locus_tag="PLI55_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB00131.1"
                     /translation="MNPEFQLTDEELISRFQAGDEGAFEEIVNRYADRLLNFAYRFVL
                     DREEAEDIVQDTFLKVYQNRHAYREIAKFSTWIYTITGNLAKTILRKRKNRKMFPFSR
                     TASEDKEREIEYPSTERPPDTKLAGEIEEQIIQRAIIKLPEHFRTVIILRDIQDLSYE
                     EISNIIDAPLGTVKSRINRARLRLQEELEHLRR"
     gene            6739..7200
                     /locus_tag="PLI55_04525"
     CDS             6739..7200
                     /locus_tag="PLI55_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00132.1"
                     /translation="MLSLPKISVSDDFKLNLRNRILSDRSAATQPNSHKGFNLSRIPS
                     FAYGFAVAVVAVVVGFFILQSQSQENSPDNMPPIVRQKMTQSPAQQPQDVTPNNPNQS
                     GLQSPQYTSKQNHPIPSDTATSNNSSSQPAEPTFEYEPNYEDQIKTVKDQR"
     gene            7280..9046
                     /locus_tag="PLI55_04530"
     CDS             7280..9046
                     /locus_tag="PLI55_04530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPB00133.1"
                     /translation="MKNKSIIIISIILFAVLFLAFIYPRLEYFSGIPVTVQTIFRILI
                     YIGVLSYLIFIIQELYHWQSRCDLAEKTLDSRRSSISRDDEFAGELSLKLKIDPDQNY
                     NEIIAQILKIAQSSLMARSAFLYLFNADEHTYILQAYQSEEGIEFCPKFKAEGTIFRE
                     LHLTLKPQIYSADGLKEGSLVYYTVPPKVGTIMVVPIMFNQEVFVGVLGLDSIDRNAW
                     GHEDVDLVKLFAELFSSSIWQIDAIDRLNTQIAFFKALCRLNTELPIGIESLELYKEA
                     SQVLHKFFNFEKLTFAVLPEEESPELYIEYVEGYDTDYSIGHRISWKGGLWEKIIKNG
                     EILLLNDYEQEKLSFRFQPGDLGILPFRSCLGVPLEVGRKRLGGILMESYQPNNYLRD
                     ESDTLVLFAKNLSEIINRMNTYQTVKDLAMIDGLTGLYNRRAFEERLQIEIERSRRYE
                     NSLTLMILDLDKFKRINDTYGHIFGDMVLKKTASIIRGSVRTVDTVARYGGEEFAVIL
                     INADKNNSYKSAERIRSNIQSFVFEKDGIREHMTISIGMAEYPIDGTDVPTMISHADL
                     AMYQSKRLGGNKVTLFKKEYAT"
     gene            9059..9262
                     /locus_tag="PLI55_04535"
     CDS             9059..9262
                     /locus_tag="PLI55_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00134.1"
                     /translation="MSKGVKGFADKVAKSSKDNKTYCPVCGESVEYVKHIETTSDPSN
                     KRSMRFKEQIVGVCKCNRDQIYQ"
     gene            9332..10849
                     /locus_tag="PLI55_04540"
     CDS             9332..10849
                     /locus_tag="PLI55_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00135.1"
                     /translation="MKKLYPSRATTILRKIVGLLVLAALVSCAATKQTSKSAEKPAES
                     SQNTQYNSQALTHFMNGENYALQGDYAMAVLEYQDALLYETKSAAIYSSLGKAYINLG
                     KLERGLAALEKACQLDPDDAETREILAQIYFLTGRFQQAEKQYQALMTLRPNEPEIMY
                     QLAAIYLKNRKIDQALQLYDEIVKVDSTQYQALQKAAEIAILNRDLNKATAYYDQLVK
                     LFPDSLLYLKTRADLAALGNDFETAIDIYKKLIILNPGDIELRERLGEILCWSDHFEE
                     AQTILEAIIDANPERKASYANLALLYVRNENYQQALDILDSARIRFPNDPSFLLAQGS
                     IYNTLRDYQSAEKVLLKTLQIEPNNVQAQHLLAAIWDMLKKYSQCDSLYEVIIRHNPD
                     DVIALNNYAYSLAVRGERLDESLKMVDLALAKEPNNASYLDTKGWILYRQKKYKEAIK
                     YIQKSLEIEQDNAEVLEHLGDVYQALRQPKKARIYYQRAAEIDKNNENLKRKLQP"
     gene            10818..11546
                     /locus_tag="PLI55_04545"
     CDS             10818..11546
                     /locus_tag="PLI55_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00136.1"
                     /translation="MKTLSVNCNRKFTGLLISLILSFSGWNCSRAPLKHEKVDEHWAP
                     ALRERSRQLKQLANLSGSGQASFSGTAGSGIIYFDFEYRTADTLNLWIKDPIGRKLAN
                     LELGGDTYHLHLIRDGRYFSGTQLETDQLPISCGPITPAIIRRILLAVPFESTTNETA
                     SQFNEASDETPVLYYKFKPGQGEPAELNWLYKNKQIIIVYKEYADVARLKIPAQIVIK
                     NKAEEIAIEIHFSHFNAEMLKFRT"
     gene            11572..12498
                     /locus_tag="PLI55_04550"
     CDS             11572..12498
                     /locus_tag="PLI55_04550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB00137.1"
                     /translation="MNSENEKKLVTVVIPVFNEVESLRELNEKIQAAFRSTNYNLEII
                     IVDDGSRDGSAQVAREIASTFPNVTVTGFQRNRGKAAALNEGFRLAKGDFVATIDADL
                     QDDPEAIPAMLDYLQESGYDLVSGWKQKRQDKFIKKTTSKIFNSVTRLLTGVKLHDIN
                     NGLKVYRAEVVKSIYLYGELHRFIPVLAKFEGFTSGEYKVKHFPRKYGKTKYGVSRFY
                     KGFLDLLTVIFITKFMRRPMHFFGLWGIVIILIGLLSELWVLILKFGFQEPFKDHVAL
                     LIFGVLAIVFGMQFFSLGLISEMITFQNRKEK"
     gene            12498..13532
                     /locus_tag="PLI55_04555"
     CDS             12498..13532
                     /locus_tag="PLI55_04555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB00138.1"
                     /translation="MPKYRFSVIIPSYNRADEVIDLLKSLQAQTCEHSRFEVLVIDDG
                     STDETEQKVNELASQVDFRLRFFRRNHEGPGPARNFGMSQAEGEYFLFIDSDCQADAN
                     WLKEIDEAITKEAPDAFGGPDSVLDSFTPLQKAIDYSMTSFLTTGGMRGHSQKRLAKF
                     YPRSFNMGIRREIYEKLGGMGNLRHGQDIEFSRRIVASGAKVIYIPNAIVYHKRRTSL
                     RKFFRQMFNWGVARINLSYLDAGNLEPLHFIPAIAAVGYTLLTIFTLLTGLAWKIWVA
                     ITVLALLICGYIFIDSSIKYKSVKVGWLAPIFAVCQIFAYGYGFTYNYVRRKIFRRGE
                     VTGFVRRYYQ"
     gene            13529..14311
                     /locus_tag="PLI55_04560"
     CDS             13529..14311
                     /locus_tag="PLI55_04560"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB00139.1"
                     /translation="MTNTPPRNLNFHKEQYWTAANFLEKYISISPVSGLRILEVGSAE
                     GGGLNYFATKNNQCYGLEYSRGRLEIARELNKSSKIIFIHGDITEASSYENQIQDKMD
                     LIICGDVIEHIANGKKAAALQNMRDLLQKGGRIYISFPPKYSPFAGHQQGLPGWLHRI
                     PYLFLLPDKFYSAILKSQGIRADIISNMLTTKHSRLSIKQFEKMVYENGLRILKKDFF
                     LIRPRFKYNYGIPYIGHRCDLPIVREIFNLGAVYLLQPNAKN"
     gene            14381..15613
                     /locus_tag="PLI55_04565"
     CDS             14381..15613
                     /locus_tag="PLI55_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00140.1"
                     /translation="METNNKSLEQVIKTVNDSLDAVLSYSQRNDYAGFNKYDALASPI
                     LLPLSFGNKNLRLFYSQAIMRSPLNLRPLFAIPRTRNPKGIALFAMTYLNLYLARKSE
                     KFLQKAEELLDWLVQNQSTGFPGNSWGYQYPWQDVGFFAPPNLPNRVVSYFVGTAFLN
                     AYEITGKNLYLNYAIGVKDFLIQAPKVLYEDDKMKCLSYVPDESINWVVMDVSALCGA
                     FCARLAHFTNDKSLIEEARKLISYVVDKQTDYGAWFYTHPPTANRLKMHDNYHTGYIL
                     DAILDYLDFSGDDSFLENYHRGLQYYLENLFLADGTPKWMNNKIYPIDVHGSAQGIIT
                     FLKAGRFNRQNEEFAYRIAEWAINNMQNKQEGFFYYQKGRFFKKPFTIMHWSNGWMAI
                     AMSEIIRKYYAENRATTN"
     gene            15585..16349
                     /locus_tag="PLI55_04570"
     CDS             15585..16349
                     /locus_tag="PLI55_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPB00141.1"
                     /translation="MPKIEQQPTKKDVEEFWNRNVCQTEFLKTGERGTEEFYQEAERV
                     RYKYHFYLPELFDWIAKEKPNGSLLEVGCSMGTDLLQLARRGMRVTGIDLTEEGINLA
                     RKRFELYQMPAELKVDDAENLSFPDNTFDVVYSFGVLHHTPDTQKAIDEVWRVLAPGG
                     LAVIMLYNRKSLNYYIHRLINQPFDGNRKDRCPIERTYTKAQIFAMFKNFKVVKIETE
                     YLLTTGYGIVWDLIPKAVHRALGHLWGWHLVIKAYK"
     gene            16349..17647
                     /locus_tag="PLI55_04575"
     CDS             16349..17647
                     /locus_tag="PLI55_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB00142.1"
                     /translation="MKKVLFISYYWPPAGGATTTRILKFYKYLPEFGWEPIVLTVEDG
                     DFPFIDPQTLKEVRPDTKIYRAKNISLHRIFSIVAKNSKEMFVPFAFTEKNNKGLKGK
                     LSRWVKYNAIPDTRFLWKRFAIKKGLQIIRENDIKLIFSSSPPQTNHCIAVELSRKTG
                     IPCVGDMRDPWTDVYWLQTNSQRWKWIHNLDQKIEKRTLNSMAAITTIGPSLVNLFQK
                     KVNKPVYLIYNGFEPLDVDFVPRKNAKWTITYAGSISIEQDIQAFCKAIEVLVQNENI
                     RENITIQFIGNFPEFVKQKLRQYSVFEMITFLPYMPADEVKRYLVSSDVLLLFIHVTP
                     EGGVINYKMYDYLATYKPILAYGPTQGDAAKILHRANAGRMFEYDNVADSANYLLSLY
                     EKWEKGEPAVEIDKEYVMTFTRRNLTRQLAEIFDKYYRKS"
     assembly_gap    17754..17853
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17870..18880
                     /locus_tag="PLI55_04580"
     CDS             17870..18880
                     /locus_tag="PLI55_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB00143.1"
                     /translation="MKTIKFALIGCGRIAQRHAEQIHRLGELSAVCDIKESAALALSE
                     QYNCRSYFDIEALLRNEPDVDVVSVCTPNYLHAEHSIKSLKAGKHVLCEKPMALSVRD
                     CENMIYTAEQTNRRLFIVKQNRYNPPVVELKKVIEENRLGKIFYVQLNCFWNRNEDYY
                     LQSDWKGRLDKDGGTLYTQFSHFIDLLVWLFGDFKNVKVLLQNYLHPNIEFEDTGAVL
                     IEFFNGAIGTINYTVNSYQKNMEGSITVFGSKGTVKIGGQYLNALEYQNIEDYSFQNV
                     TPVNKANDYGFYQGSMSNHDKVYENVVNVLNGKDTIATNAIEGLKVVRIIEDIYSQSK
                     LK"
     gene            18883..19347
                     /locus_tag="PLI55_04585"
     CDS             18883..19347
                     /locus_tag="PLI55_04585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB00144.1"
                     /translation="MAEPIIKKIGIRDIKCGTNVVIIEPCNLYECEIGDNTFIGPFVE
                     IQKGVTIGRRCKIQSHSFICELVTIGDDCFISHGAKFVNDTFKSGKPAGGNRELWKPT
                     IIGNHVAIGTNATILPVKITDRVVIGAGSVVTKDILHSGIYAGNPARKIRDL"
     gene            19363..20457
                     /locus_tag="PLI55_04590"
     CDS             19363..20457
                     /locus_tag="PLI55_04590"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB00145.1"
                     /translation="MIPFMDLETQYLNIREEIDSAIFNVIHSYKFINGPEVATFEKNF
                     ATAQNLKYMLGLASGTAALHLAYEILGITSGDEVIVPAMTFIATAETVRQVGAKPVFA
                     DIDPVSFNIDCNGLEKLITPKTKAIVVVHLHGNPCEMDRIVSFAKKYNLKIIEDCAQA
                     HLAEFQGQKVGTFGDVAAFSFYPGKNLGAYGDAGALGTNDETLYEKAKLLANHGRKEK
                     YVHQTEGYNYRLDTLQAAILDVKLRHLPEWTEQRINIANTYHSLLKNLPITLPEMNPA
                     KKHVYHIFAIATDRRQRIIEALKANQIAFGIHYPLPLHLQPAYSYLHYKPGDLPNSEL
                     LSTQFLSLPMYAELSRDNIEKICQVIASVY"
     gene            20551..>21378
                     /gene="asnB"
                     /locus_tag="PLI55_04595"
     CDS             20551..>21378
                     /gene="asnB"
                     /locus_tag="PLI55_04595"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPB00146.1"
                     /translation="MCGICGLVDPNENNHRLITEMTDLLKHRGPEKAGYFHDGNVSLG
                     HRRLAIIDLETGDQPIYNEDRTVVVIMNGEIYNFPEFKADLIQRGHHFYTNSDTEILA
                     HSYEEYGLEFLARLNGIFAFALYDIKDERLILARDHFGVKPLHYWFQNEVLLFSSEYK
                     AILVHPAVRRQVNYSALHCQVNLRYNQSDETLIKGIYRLPPASYLIYEKGQISIKKYY
                     QLPVNLNFKMTEAEAIDGIRFHLQQAVRRQLISDVPLGVYLSGGMDSSALVAMMAAEK
                     "
BASE COUNT         6307 a         4225 c         4680 g         6066 t
ORIGIN      
        1 tcgaaaaggt gtttgttgtg attgcgttcg ttatcatctg cggtcgaggc aactaccggc
       61 ttgttttttc cccgatgatg ttgagcgcac ttatgatcgc tcttttgaaa aattcactga
      121 attagtccaa agaggaaaag tatagccgat aaaagaaatg aaacgtcatt tttactggtt
      181 aaaattaatt atttgcgagg ctttgatgtt caatttaaac aatttagcgg ctgaacccgg
      241 agaaataagg caacttccca agcctgatgc agtcggtaga gtcccaattg agacgacttt
      301 gagccaacgc cgttcggtgc gaacattcca gaatgagccg ttgtccgaaa aggaaattgc
      361 ccagttgctt tatgcggcgc agggaatcag cgactcttca cagggattgc ggacggcgcc
      421 ctcggccggg gcaacttacc cgctggaaat ttatctcgtg acagcggagg gaatttttca
      481 ctatttgccg gaacgccatg ccatcgtatt aaaaaaagcc ggtgattgtc gcgatcattt
      541 agccgaagcc agtctgggac agaattccgt tcgtactgca ccagccatca tcgtttttag
      601 tgcagtttat gagcggacga tgcgccgtta tcataagcgt ggtgagcgct acgtccatat
      661 agaagttggt catgccgcac aaaacgttca tctacaggca gtggcgctcg gactggcttc
      721 tgtgccggtg ggagccttta atgacgaggc agtcgccaga gtcattggtg ttgaacggaa
      781 cgaacgagtg ctttatctag tgccggttgg ccgaaagcgc tgaacctccg tatattttat
      841 aaaaaaacat cacattccgc tggcgaatgc agaaaattta tcttatttat cgataagttt
      901 tcagtcagct ataagaaaac cgatgccttt ctactcttaa ttggctaaat ttcacttaat
      961 atttgatatt tcaaaaaatt ggagtctaaa gaatgttacg tagtcaggat tatattaaga
     1021 tcgaagatca gtatggtgct cataattacc agccgctgga cgtagttttg acccgcggtc
     1081 agggaatctg gctcgaggat gtcgaaggca aaaagtatat ggattttctg gcggcttact
     1141 cggcggtcaa tcaggggcat tgtcatccac gtctggtcaa agttatgcaa gagcaatgtg
     1201 cccgtctgac gctgacctcg cgcgcttttc gtaatgacca atggccgcta ctagccaaag
     1261 aactttgtga actaactggt tacgaaatgg tattaccgat gaactccggg gcggaagcag
     1321 ttgaaacggc gatcaaagcc gcccgcaaat ggggttattt atacaaaggc gttgccagag
     1381 acaaagctga aatcatcgtt tgtacgggca attttcacgg tcgcaccacg acgattattt
     1441 ctttcagcac cgatgaacaa tacaaagacg gatttggtcc gctaacgccc ggctttgtcg
     1501 ccatagaata tggttcgatt gatgagctaa aaaaggctat caatcccaat acggttgcct
     1561 ttctggtcga accgattcaa ggcgaaagcg gtgttatcgt gccaccggag ggatggctaa
     1621 aggcatcttc cgaaatttgt cgcaaaaata aggttttgtt aattgccgat gaaattcaga
     1681 gcggtctggg gcgcacgggt aaactctttg cctgcgaaca tgaaaatgtt cgaccggacg
     1741 tagtaattat tggcaaagcg ctttccggcg gaatgtatcc cgtctcagcc gtagttgctt
     1801 ctcaggaaat tctgggggtt ttcaaacccg gcgatcatgg ctcaacctat ggtggaaatc
     1861 ccttaggcag tgccattgct cgcgaagctc tcaaaatcat tattgacgag aaattgccgc
     1921 agaaatccgc cgaatccggt gcttatttaa tgcaaaaatt aaatgaaatc gagagtccct
     1981 acatcgaaca aattagaggc aaaggtttat gggttggcat cgttttgaaa ccatcagccg
     2041 gaggcgctcg ccacttctgt gtggcgttgc aggagcgtgg cattctggca aaagagactc
     2101 atgaaaacgt cattcgccta gcgccgccgc taaatattac ccgaagtgaa ctcgaccatg
     2161 cgatcgcaat attgcaagaa gttttgactc agtaatatct gaaaaaaata aactcataag
     2221 agttaaaaac tagcaggagg tttatttgaa agaagcaatc gaaggattaa aaccggccag
     2281 tctatggcgc tatttttctg aaatgagcca gattccgcgc gaatccaaac atgaggaagc
     2341 cgtaattgcc tggctcgaat cagttgctaa aaaattgaat cttccctatc gcaaagatga
     2401 tgtcggtaat attgtcattt ctaaaccggc ctctcccggt tatgaaaacc gcacgccggt
     2461 agttatacag gggcatgttg atatggtttg tgagaagaac agcggcactg tccatgattt
     2521 ccgaaaagac ccgatagaat tggttcgtga cggcgagtat ataagggcta atggtactac
     2581 tctcggtgcc gataatggca tcggtgtagc ggccgccctg gcgattcttg aggataaaac
     2641 tttagcacac ccgccgctcg aagctctttt taccattgac gaggaaaccg gcttaacggg
     2701 cgcaaactcc ttaaagcctg gatttattaa gggcaaaatc ctactgaatt gtgattcgga
     2761 agaagatggc gctttgtatg tcggttgtgc cggtggaaaa caaaccgatc tatttttcga
     2821 tattaactgg gaaaaagcgc ctcaacaaat ggctaatgtt gccctaaaaa tttccgggct
     2881 gaaaggcggt cattccggac tggatattga ccaggggcgt gccaatgcta tcaagcaatt
     2941 gaaccgcata gtttggaaag ccgctgagaa atatgacttt cggctttatt acttggaagg
     3001 cggcagtaaa cataatgcca ttccgcgcga agccgtttat aaattgctga taaaaccggc
     3061 tgatttagct aatttcgaga ccgagattgc caaatttgaa aaaatattaa ataacgagta
     3121 taaagacgtt gaacgtggca ttaaactgac ggttgaaaat ttaaatgaat acccgaagca
     3181 ggttttcacg aaggaatttc aagcgcgagt attgaactgc ctctatgcca tcccgcacgg
     3241 tgtcaagaga atgagcccca caatcaaggg attggtgcag acctccaata atgccgctat
     3301 agtcagtact ggtgataagc agatcagagt tcttaccagt cagagaagtt cggttgcctc
     3361 cgaaatcgag ggcttagctg ataaagtcaa agcttgtggt cttttagcca acgccgaagt
     3421 agttcaaagt ggaggttatc cgtcttggac tcctaatatg gattcgaaga ttctaacatt
     3481 gatgaaaaag gtttatgccg atctttttgg cgttgaaccc caggtcaagg ctattcacgc
     3541 cggactggaa tgtggtatta tcggggctac ttcaccgggt atggatatga tttcatttgg
     3601 tccaactatc gagggggcac attcgccaga cgaacgcgtc gaaatcaaga ccgtcgagaa
     3661 attctggcgg ctgttggtcg agacactcaa acgaatcgaa taattgctag attcgcatag
     3721 gttgaaaacc accacgaaag ctctaagaca taaagtattt aatttcctct tgaaagtaat
     3781 tcagaatgct ctgataatat cggagcgtct caggatagag cgaggtgaac agttcagcct
     3841 ttaggttgtg cgcataaatt gatactgtca atttaatccc tactatcagg cataacgaga
     3901 aagattgcaa ccttcagaag gtctgataac caatttacca aaccgacctt cttcaggtta
     3961 acaaatgcta cttccaccga tcagccttgg tgaaatgtta cattcatttg atttggctca
     4021 aattgattta aataattttt ggtcgagatt aaaagatatt tagtcattat ggattggtaa
     4081 aacaatcttg gtaaaccggc tagctcggaa taaatttact atatttggag gtttatgaat
     4141 tactttgaat cttttatttt gggaattgta caggggctga cggagttcct gccgattagc
     4201 agttcaggac atttggtaat cgttcagcat ctctttgggt tagcgaccga caatattgcc
     4261 tttgaagtta tggttcacgc cggcacttta ttgagtgtgc tggcggttta ttacaatgac
     4321 gtatggcgga tgatagtaag tttttgtcgt ggaattttcc ggctacgcaa agcgcaaccg
     4381 tttcagaatg attcttattt tcggctgagc atttttgtaa ttgtggctac tattccggcg
     4441 gtaatattcg gcttgacact ggacgacttc ttcgagcaaa ttttccatag cgtattttta
     4501 gtcggcatta cgttgacgat taccggtttg attcttttat caacgcggtg ggtaaaaacc
     4561 gaaggccgcc gattgacttt tggtaaaagt ctcttgattg gttgtgccca ggcggtagcg
     4621 attttgcccg gtatttcgcg tagcggttcg acgatcagcg gcggtttgtg gtgtggcttg
     4681 gatcggatgg aagccacccg tttttcgttt ctgctctcgg tgccggcaat tctgggcgca
     4741 ttggttttgc atctcaaaga tctgattggt atggctctca atcccaatat gattgggata
     4801 ttaatccttg gatttttaac ctcttttatt gttggatata ttgctattcg atttttacta
     4861 agagtagtgc aaagtgataa attcagctgg tttggtatct attgcataat agtcggactg
     4921 acagttgtat ttttcctcga ataaaagttt aactaataag aagcccaagg aatccaatgg
     4981 caatgtcgaa gagtgatttc ttaagtgaat tagcgagaat tgagcaaggt catctcgatg
     5041 cggtttattt attacggggt ggtgatcttt atctggagga cgaagccatc aataccattt
     5101 gtcaggcttt tggcaaacaa gaatctgaga ggccggaaaa actgatttac tacggcggtg
     5161 ttgatagcga tctcgaattc attgataatc ttttcagtat gggatttttt gccaggcgcc
     5221 ggattattat ctataaaaat attggtaatc ttgagccaaa tctgcgtgct cggcttttta
     5281 attttatcga aaaccccgga caggatactg ttttaattat gactgccgat ggcgaaagtc
     5341 attccagcct aattgaacaa ttgcgtaaag aatcgcggaa ggtcaaaacg atttcgactt
     5401 ggacgcccaa accctccagg tttgatgaat tcatccgacg ccacctccaa aaaagtcaat
     5461 attccatccg accggaagcg ctcaaattgc tgatagaatt gaccgatgat gcgctcagcc
     5521 atacgatcgg tgaattggaa aagttgctga tttacattgg cgagcggaaa atgatcgaag
     5581 aagatgatgt tcgtctaatt gttggcggcg ataagaatta cgatatgatt aatttccttg
     5641 aagctgtagc tcaccgcgac ataaccaaag caataaagat cggtttggct ctaatccaag
     5701 ctgatgcatc aatcccctat tttaccaccc aattgtatga cttattcagc ggcatttggg
     5761 attatgaggg acatggtcaa aaagaaaaat tctggaagaa aagtaattgt tatgagctgg
     5821 gaataaagaa ctataaacag gctgattttg gtgcaatttt tacctgtatt agaaatgtgg
     5881 atttacaatc aaaatcttta aatttgaata aagaagattt attaattcaa ttgttgtatg
     5941 aaatcatctc ctaagaaaaa aacagaaatg aaccccgaat ttcaactgac cgatgaagaa
     6001 ttgatcagcc gttttcaagc cggtgatgaa ggcgctttcg aagaaattgt caaccgttat
     6061 gccgaccgtt tgttgaattt tgcctatcgt ttcgtcctgg atcgggaaga agcggaggat
     6121 attgtccagg atactttcct taaagtttat caaaaccgcc atgcctatcg tgaaatagcc
     6181 aaattttcca cctggattta cactattact ggcaatttgg ctaaaaccat tctgcgcaag
     6241 cgcaagaatc gcaaaatgtt tcccttttcg cgtacagctt cagaagataa agagagagaa
     6301 atcgagtacc cctccaccga acgtccgccg gatactaaac tggcaggcga gatcgaggaa
     6361 caaataattc agcgggcaat tataaaatta ccggaacatt ttcgtaccgt aatcatttta
     6421 agggatattc aagatctttc atatgaggaa attagcaata tcattgacgc gccattaggg
     6481 acggtcaagt cacggatcaa ccgggcgcgg ctgagattgc aggaagaact tgaacattta
     6541 aggcgataga gaagatgatt agtaaaaaat gggggaaatc aaaaatttat gatgaattgc
     6601 taccaattta ggaattccat atcaaatttt attgacgaaa acatctcctt caaaaaccgt
     6661 caagctttcc aagagcacct gaatgaatgc ccagtttgcc agaaaattta tagctccatc
     6721 ttggcgaccc ggcaaaatat gttaagctta cctaaaattt ctgtaagtga cgatttcaaa
     6781 cttaacctac gcaatcggat tctttccgat cgcagtgcgg ctactcagcc taactcacat
     6841 aagggtttta acttaagccg aatcccgtca tttgcttacg gctttgccgt agcagtggtt
     6901 gccgtggtag tcgggttttt tattctgcaa tcccagagtc aggaaaattc gccggacaat
     6961 atgccgccga tcgtacgtca aaaaatgacc caatctccgg ctcaacagcc tcaagatgtg
     7021 acgccgaata atccgaacca atccggttta cagtctcctc aatatacctc taaacaaaat
     7081 cacccgattc cgagcgatac tgctacttcg aataattctt cgagccaacc agctgaaccc
     7141 acgtttgaat atgagccaaa ttatgaggat cagattaaaa cggttaagga tcaacggtga
     7201 tttgacgctc ctgacaatat tattttttgg ttaaacctaa tttttaaagt atttttacac
     7261 ccaatatggg tgttttttta tgaaaaataa aagtatcata ataatcagta taattctatt
     7321 cgcggtactg tttttggcgt ttatttatcc ccgtttggaa tatttcagcg gaataccggt
     7381 aacagttcaa actatctttc gaatactgat ttacatagga gttctgtctt atttaatttt
     7441 cattattcag gaattgtacc attggcagag tcgctgtgat ttagccgaga aaactctgga
     7501 ctcccgccga tcttccatca gccgggatga cgaatttgct ggtgagctta gtctgaagct
     7561 gaaaattgat ccggatcaga attacaacga aattatcgcc caaatattga agatagccca
     7621 atcttcttta atggcgcgta gtgctttcct ctatttattt aatgccgatg aacatactta
     7681 tattttgcag gcttatcagt ctgaagaagg aattgagttt tgtcccaagt tcaaggctga
     7741 aggcactatt ttccgggaat tgcatctgac tttaaaacca cagatatact cagccgatgg
     7801 tcttaaagaa ggtagtttgg tttattatac tgttccgccc aaagtcggca cgatcatggt
     7861 cgttcccata atgttcaatc aggaagtatt tgtcggtgtc cttggacttg atagcattga
     7921 ccgtaatgcc tgggggcacg aagatgttga tctggtgaaa ctctttgcgg aattattttc
     7981 ctcctcaatc tggcaaattg acgccattga ccgactcaac actcaaattg cctttttcaa
     8041 agccctttgc cgcttgaaca cggaattacc tattggaatt gagagtcttg aattatacaa
     8101 agaagccagc caggttttac acaaattttt taattttgaa aaattgactt ttgccgtttt
     8161 acctgaggag gaatcacccg aactctatat tgagtatgtc gaaggttacg atactgacta
     8221 tagtatcggt catcgtattt cctggaaagg cggcctctgg gagaaaatca tcaagaacgg
     8281 tgagatactc ctgctgaacg attatgaaca ggaaaaacta tcgtttcgtt ttcaaccggg
     8341 agatttaggt attctgccct ttcgttcctg tctgggagtg ccgttggaag tagggcgcaa
     8401 gcgactgggt ggcattttga tggaaagcta tcagcccaac aattatctac gggatgaaag
     8461 cgataccctg gttttatttg ctaaaaacct cagcgaaata atcaatcgga tgaatactta
     8521 tcagacggtg aaagatctgg cgatgattga tgggcttacc ggtctctata atcgtcgagc
     8581 tttcgaagaa cgtctccaga ttgaaatcga gcgcagtcgt cgttatgaaa attccctgac
     8641 cctgatgatt ctcgatttag ataagttcaa aagaatcaat gacacgtacg ggcatatctt
     8701 cggggatatg gttttgaaaa aaacagcctc tattattcgc ggaagtgtcc ggacagttga
     8761 tacagttgcg cgatatggtg gcgaagagtt cgctgtaatt ttgattaatg ccgataaaaa
     8821 caacagttat aaatcagctg aaagaattcg cagtaatatc cagagctttg tatttgagaa
     8881 agacggaatt cgcgaacata tgacaattag tatcggtatg gcggaatatc cgatagatgg
     8941 caccgatgtt ccgactatga tttcgcatgc cgatttagca atgtatcaat caaaacgtct
     9001 gggtggcaat aaagtaacct tatttaagaa agaatatgca acttaaaagg agtcaattat
     9061 gagtaaaggt gtcaaaggat ttgccgataa agtagccaaa tcctcaaaag ataataaaac
     9121 ttattgtccg gtttgtgggg aaagcgttga atatgtaaaa catattgaga ctacttccga
     9181 tccgtccaat aaaagatcaa tgcgttttaa ggagcagatt gtcggggttt gcaaatgtaa
     9241 ccgcgatcaa atttatcaat aaccataaga ccactctctt gtcaagcccc gtttttttgg
     9301 gctggcttag ttagagtggg aagtgaaaaa catgaaaaaa ctatatccaa gcagggcgac
     9361 aactattctc aggaaaatag tcggtttgct tgtattagcc gctctggtta gttgtgccgc
     9421 taccaaacaa accagtaaat ctgcggaaaa gccggctgaa tcatctcaaa atactcaata
     9481 taattctcag gctctgacgc attttatgaa tggtgagaat tatgctctac agggcgatta
     9541 tgctatggca gtactggaat accaggatgc acttctttac gaaactaaat cggcggctat
     9601 ctacagctct ctcggcaaag cctatattaa tctcggcaaa ctggaacgtg gcttggcggc
     9661 tctggaaaaa gcctgccaat tggatccgga tgacgccgaa actcgcgaaa tcttagccca
     9721 gatttatttt ctgaccgggc gttttcaaca ggctgaaaaa cagtatcagg ctcttatgac
     9781 tttacgaccc aatgaaccgg aaataatgta tcaattagcc gctatttatc tcaagaaccg
     9841 aaaaattgat caggctttgc aactctacga tgaaattgtg aaagttgatt cgactcaata
     9901 tcaagccctc cagaaggcgg cggagatcgc tattctcaat cgcgatctaa ataaagccac
     9961 cgcttattat gatcaattgg taaaactatt tcccgatagc ttattgtatt taaaaacccg
    10021 agcggattta gcagctttgg gcaatgactt tgaaacagcg attgacatct acaaaaaatt
    10081 gattatttta aatcccggcg atattgaatt aagagaacga ctcggagaaa ttttgtgctg
    10141 gagcgaccat tttgaggaag ctcaaacaat cctcgaagca attatcgatg ctaatcctga
    10201 gcgcaaagcc agttacgcca atctggcatt actttatgtt aggaatgaaa attatcaaca
    10261 ggctcttgat attctcgatt ctgccagaat ccgtttcccg aatgatccat cgtttttact
    10321 cgctcagggt tcgatttaca ataccctgcg cgattatcaa tcggcagaaa aggttctgct
    10381 gaagacttta cagattgaac caaataatgt ccaagctcaa catctgctgg cggcaatctg
    10441 ggatatgtta aaaaaatatt cgcagtgtga tagcctttat gaagtaatta tacgccataa
    10501 tcccgatgac gtcatcgcct tgaacaatta tgcttacagt ctggcagtgc gtggtgaacg
    10561 actcgacgaa tccttaaaaa tggtggattt ggctctggct aaagagccca ataatgcctc
    10621 ttatctggat accaaaggct ggattctcta ccggcagaaa aaatataaag aagcaataaa
    10681 gtatattcag aaatctctcg aaattgagca ggacaatgcc gaggtactgg aacatcttgg
    10741 cgacgtctat caagccttac ggcaacctaa aaaagcccga atttactacc aacgagccgc
    10801 cgaaattgat aaaaataatg aaaaccttaa gcgtaaactg caaccgtaag ttcacaggtt
    10861 tactaattag cctgatctta tcgtttagcg gctggaattg tagtcgagcc cccctcaaac
    10921 atgaaaaagt tgatgagcat tgggcgcccg ccttgagaga acgcagtcgg caattgaaac
    10981 agctggctaa tctttccggt tctggccagg cgagttttag tggaacggct ggtagcggaa
    11041 taatctattt tgattttgag taccgaaccg cggatacatt gaatctttgg atcaaagacc
    11101 cgatcggaag gaagttggct aatcttgaat taggcggtga tacttatcat ttacatttaa
    11161 tccgggatgg taggtatttt agcggaacgc aattagaaac tgaccaatta ccaatttctt
    11221 gcggtccaat cactcccgca ataatccgac gaattttact agccgtgcct tttgaatcta
    11281 ccaccaacga aaccgcttct cagttcaatg aggcaagtga tgaaacgcct gttctgtatt
    11341 ataaatttaa acccggtcag ggtgaaccag ctgaacttaa ttggctttac aaaaacaaac
    11401 agataattat tgtttataaa gaatatgctg atgttgctag gttaaaaatt cccgctcaaa
    11461 tagttatcaa aaacaaggcg gaagagatcg caatagaaat tcatttctcc catttcaatg
    11521 ccgagatgct taaatttcgg acgtaatata ttcgccatta tcgaggtaaa tgtgaattcg
    11581 gagaatgaaa aaaaacttgt tacggtagtg atcccggttt tcaatgaagt ggaatcactg
    11641 cgcgaactga acgaaaaaat tcaagccgct ttccgttcaa cgaactataa cctggaaata
    11701 atcattgttg acgacggcag tcgcgacggc tcggcacaag ttgccaggga aatcgccagc
    11761 acttttccca atgtaacggt taccggtttt caacgtaatc gtggtaaagc cgccgccctg
    11821 aatgagggtt tccgtctagc aaaaggtgac ttcgtggcta ctatagacgc cgatttgcag
    11881 gatgatccgg aagccattcc ggccatgctg gattatctcc aggaatcagg ctatgatttg
    11941 gtatctggct ggaaacaaaa gcgccaggac aaatttatta aaaaaacaac ctcgaaaatt
    12001 tttaattcag tcactcgctt gttaactggt gtgaaactgc acgatatcaa taatggtcta
    12061 aaagtttacc gtgccgaggt cgtcaaatcc atatatctgt atggcgaatt gcatcggttc
    12121 atcccggtat tggctaaatt tgagggattc accagcggcg agtataaagt caaacatttt
    12181 ccacgtaaat atggcaaaac aaaatacggt gtcagtcggt tttataaagg tttcctcgat
    12241 ttattaacgg tgatctttat tactaaattt atgcgccgac cgatgcactt cttcggatta
    12301 tggggaatag taattatatt gattggcttg ctatcggaat tatgggtttt gatcctaaaa
    12361 ttcggatttc aggagccatt caaagatcac gtagctttgc tgatttttgg ggtgctggcg
    12421 attgttttcg gtatgcagtt tttttcactt ggcttgatta gcgagatgat tactttccag
    12481 aacaggaaag aaaaataatg cccaaatata ggttttccgt cattattccg tcatataatc
    12541 gagcggacga agtaattgat ttactgaaat cgttacaagc ccagacttgc gagcatagtc
    12601 gtttcgaagt actggtgatt gacgatggtt cgacggacga aacggaacaa aaagttaatg
    12661 aattggcatc gcaagttgat tttcgcctga ggttttttcg acgcaatcat gaaggtcccg
    12721 gtccggcgcg gaactttggg atgagtcagg cggaaggcga gtatttcctc tttatagatt
    12781 cagactgcca ggcggatgcc aactggctga aagaaattga tgaggctatt acgaaagaag
    12841 cgccggacgc atttggtggt ccagactcgg tgctggattc ctttacgcca ttgcagaagg
    12901 caattgacta cagtatgaca tctttcctta ccaccggtgg aatgcgtggt cactcgcaaa
    12961 aacggctggc aaaattttac ccgcgcagtt tcaatatggg catccgacgt gagatttatg
    13021 aaaaattagg tggtatgggt aacctacggc acggtcaaga tattgaattc agtcgccgaa
    13081 ttgtagccag cggcgctaag gttatttata taccgaacgc catagtttat cataaacggc
    13141 gcactagcct gcgcaaattc tttcgtcaga tgtttaactg gggagtggcg cgtatcaatc
    13201 tgagttatct ggatgcgggc aatttagaac cgttgcattt tattccggcg atagccgcgg
    13261 tcggctatac attattgacg atatttacct tactgactgg tttagcctgg aaaatatggg
    13321 ttgcaattac cgttctggca ctgctaatct gtgggtacat tttcattgac agctcgatta
    13381 aatataaatc cgtgaaagtc ggctggctgg cgcctatttt tgcagtttgc cagatttttg
    13441 cttatggtta tggatttact tataattatg ttcgccggaa aatcttccgc cgcggcgaag
    13501 ttaccgggtt cgtgcggaga tattatcagt gacaaatact cccccccgaa atctcaattt
    13561 ccataaagaa caatactgga cagcggccaa ttttttagaa aaatatataa gtatttctcc
    13621 tgtttcagga ctcagaattc ttgaagttgg ttcggcggaa ggcggtgggt taaattattt
    13681 tgcgactaag aacaatcaat gttatggttt ggagtattcg cgcgggcgct tagaaatcgc
    13741 ccgggagtta aataagtctt ctaagattat ctttattcac ggtgatatta cggaagcaag
    13801 ttcctacgag aatcaaattc aggataaaat ggacttgatc atatgcggtg atgtgattga
    13861 acatattgcc aatggaaaaa aggcggcggc actccaaaat atgcgggatt tattgcaaaa
    13921 aggcggtcgc atttatattt cctttccacc gaagtattcg ccttttgcgg gacatcagca
    13981 ggggctaccg ggttggctac atcggattcc ctatctcttt ttattgccgg ataaatttta
    14041 ttccgcgatt cttaaatcgc agggaattcg cgctgacatt atatcgaata tgttgacaac
    14101 caaacattct cggctttcaa taaaacaatt tgaaaaaatg gtttatgaaa atggactacg
    14161 aattctgaaa aaggattttt tccttatacg tccccgtttt aaatataatt atggtattcc
    14221 ttacatagga catcgctgtg atttaccaat tgttcgagag atttttaacc tcggcgcagt
    14281 atatttactt caaccaaacg caaaaaatta atggtagtat gtcaatctca gccagatgaa
    14341 ataaatatca gaatcattaa agattaaaga tattatctga atggaaacaa ataataaaag
    14401 cttagaacaa gttataaaga cagtaaatga ctcattggac gccgtgcttt cctattcaca
    14461 gagaaatgat tatgccggat ttaataaata cgacgcttta gcgagcccga ttttattgcc
    14521 attatcattc ggcaataaaa atttgagatt attttatagc caagcgatta tgcgttcacc
    14581 gctaaattta cggcctttat ttgccattcc gcgcaccaga aatccgaaag gaatcgccct
    14641 ttttgcaatg acctatttaa atctatactt agcaagaaag tccgagaaat ttcttcaaaa
    14701 agcagaggaa ttactggatt ggttagtcca aaatcaatca accggatttc cgggcaattc
    14761 ttggggatac caatatccct ggcaagatgt cggctttttc gcaccaccta atttgcctaa
    14821 ccgcgtggtg tcgtattttg tcgggacggc tttcctaaat gcttatgaaa ttaccggaaa
    14881 gaatttatat ttaaattatg cgataggagt aaaagatttt ttaatccaag ctccgaaggt
    14941 cttatatgaa gacgacaaga tgaagtgttt aagctatgtg ccggatgaaa gcatcaattg
    15001 ggttgtaatg gatgtgagtg ctttatgtgg agcattttgt gctcggttag cccattttac
    15061 caatgataaa tccttgatcg aagaagcccg taaattaatc agttacgtcg ttgataagca
    15121 aaccgactat ggcgcctggt tttatactca tccaccgact gccaatcgtt taaagatgca
    15181 cgacaattac cataccggat atatattgga tgcaattctg gattacttag attttagtgg
    15241 agatgattcg tttcttgaaa attatcatcg cggtctgcaa tattatttag agaatttgtt
    15301 cttagccgat ggaaccccaa aatggatgaa taacaaaatt tatcccattg acgtccacgg
    15361 ttcggctcag gggatcatta cttttctaaa agctggccgg tttaatcgcc aaaatgaaga
    15421 atttgcttac cgaatcgccg agtgggctat caataatatg caaaataagc aagaaggttt
    15481 tttttattac caaaaaggaa gattttttaa aaaaccattt actattatgc attggagtaa
    15541 tggctggatg gcaatagcaa tgagtgaaat aatcaggaaa tattatgccg aaaatagagc
    15601 aacaaccaac taaaaaagac gttgaggaat tctggaatcg gaatgtttgt cagactgaat
    15661 ttctcaaaac tggtgagcgc gggacggaag aattttatca ggaagccgaa cgtgttcgat
    15721 ataaatatca cttctatcta cctgaattat tcgattggat cgctaaagag aaaccaaatg
    15781 gttcactcct tgaagttggc tgtagtatgg gaactgattt attgcaactt gccaggcgcg
    15841 gaatgcgagt aaccgggatt gacctgacgg aagaaggaat taatttagcc cgtaaacgct
    15901 ttgaactata tcagatgcca gctgagctga aagtagatga tgcggagaat ctctcctttc
    15961 cggataatac tttcgatgtt gtctattctt tcggagtttt acatcatact ccggataccc
    16021 aaaaagccat tgatgaggtc tggcgggtat tggctcccgg tggtttggcg gtgattatgc
    16081 tgtataatcg taaatcgctc aattattaca ttcatcgtct gattaatcag ccttttgacg
    16141 gtaatcgtaa agatcgctgt ccgattgaaa gaacctatac caaggctcaa atattcgcaa
    16201 tgtttaaaaa ttttaaagtt gtgaaaatcg agactgaata tttattaacc accggatatg
    16261 gaattgtttg ggatttgatt cccaaagcag tccatcgcgc acttggccat ctctggggct
    16321 ggcatctggt aataaaagca tataaataat gaaaaaggtt ctgttcattt cttattactg
    16381 gccaccagca ggaggcgcga ccacgacaag aatcctcaag ttttacaagt atctacctga
    16441 attcggttgg gagccgatcg ttttgactgt cgaggatggc gatttcccat ttattgatcc
    16501 tcaaacattg aaagaagtca gaccggatac aaagatctat agggctaaga atatctcttt
    16561 acatcggatt ttctcaatag tcgcgaagaa ttccaaagaa atgttcgttc catttgcttt
    16621 tacagagaaa aacaataaag gcctaaaagg aaagctatca cgctgggtaa aatataacgc
    16681 tattcccgat acgcgtttct tatggaaaag attcgcgatt aaaaaaggtt tacagatcat
    16741 tagagaaaat gatattaagc taatcttttc ttcatctcca cctcagacga atcactgcat
    16801 tgcagttgaa ttatcacgaa aaacaggtat cccgtgcgtc ggcgatatgc gagacccgtg
    16861 gacggatgtc tattggttac agaccaattc ccaacgctgg aaatggattc acaatctgga
    16921 tcaaaagatt gaaaaacgga ctctcaattc aatggccgct attaccacaa tcggtccatc
    16981 gttagtcaat ttatttcaaa agaaagttaa taaaccggtt tacctaatct ataatggttt
    17041 tgaaccactt gatgttgact ttgtcccgcg caaaaatgca aaatggacta tcacttatgc
    17101 cggaagcatc agtattgaac aggatattca ggcattttgt aaagccattg aagttttggt
    17161 tcaaaatgaa aatatcagag aaaatattac aattcagttt atcggcaatt tccccgaatt
    17221 cgtaaaacag aaactgcggc aatattccgt tttcgaaatg atcactttct tgccttatat
    17281 gccagccgac gaagtcaagc gataccttgt ttcttcagat gtacttttac tgtttattca
    17341 cgtgactccc gaaggcggag taatcaatta taaaatgtat gattatctcg ctacgtacaa
    17401 accgattctg gcttatggac cgactcaagg cgatgccgcg aaaatcctac acagagccaa
    17461 tgccggtaga atgtttgaat atgataacgt cgcggattct gctaattatt tattgagttt
    17521 atatgaaaaa tgggaaaaag gtgaaccggc tgttgaaata gataaggaat atgtaatgac
    17581 ttttacccgc cgcaacctta cgcggcaact ggctgaaatt tttgacaaat attacagaaa
    17641 atcctgaatc gaatcatttt atcttttcaa ttgattctca gccaattttc actaattttc
    17701 gatatatgcg tgatgtgctt tttgaaagag ttaattaagg ataaaaccaa tgcnnnnnnn
    17761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaaaggg atttgataat tgaagacaat
    17881 aaaatttgcg ttaataggat gtggtcgaat tgcgcaacgc cacgccgaac aaattcatcg
    17941 tctgggcgag ttaagcgccg tctgcgatat taaagaaagt gcggcattag cattatcgga
    18001 gcaatataat tgccgctctt attttgacat tgaagccctt ttaaggaatg aacccgatgt
    18061 ggatgtcgtt tcggtgtgta cgcccaacta tctgcatgcc gaacattcca taaaaagtct
    18121 gaaagccgga aaacatgttc tctgcgagaa acctatggcg ctttcagtgc gcgactgtga
    18181 aaatatgatt tatacagctg aacaaaccaa ccgacgtctc ttcattgtca aacaaaatcg
    18241 ctataatcct cccgttgtgg aattaaaaaa agtcatcgaa gaaaatcggt taggtaagat
    18301 tttttatgtc caattaaact gtttctggaa tagaaatgag gattattatt tacaatccga
    18361 ctggaaagga cggctcgata aagacggtgg caccctttat acccaattca gccactttat
    18421 tgatctattg gtctggttat ttggcgattt caaaaacgta aaagtgcttc ttcagaatta
    18481 tctccatccc aacattgaat ttgaagatac cggcgccgtt ttaatcgaat ttttcaatgg
    18541 agccatcggg actattaatt acactgtaaa tagctaccaa aaaaatatgg aaggttccat
    18601 aaccgtgttc ggtagtaagg ggacagtgaa aatcggcggt caatatttaa acgcacttga
    18661 atatcaaaat atcgaagatt attctttcca gaacgttacg ccggtcaata aagccaatga
    18721 ctatggattt taccaaggct cgatgtcgaa tcatgataaa gtttacgaaa atgtcgttaa
    18781 tgtattaaat ggcaaagata ccatcgctac taacgccatt gaaggactga aagtcgttcg
    18841 aatcattgaa gacatttatt cgcaaagtaa attgaagtaa atatggctga accaattata
    18901 aaaaaaatcg ggattcgtga tattaaatgt gggacgaatg tagttattat tgagccgtgt
    18961 aatctctatg aatgtgagat tggtgataac actttcattg gtcctttcgt tgaaatccag
    19021 aaaggtgtca caattggtcg gcgctgtaaa attcagtccc attcgtttat ttgcgaatta
    19081 gtaacgatcg gcgatgattg tttcatttct catggagcta aatttgttaa tgacacattt
    19141 aaatcaggta aaccagctgg tggtaatcgg gagttatgga aaccgaccat tatcggtaat
    19201 cacgtcgcca tcggtactaa tgccaccatt ttgccggtga aaattaccga ccgagtcgta
    19261 attggagccg gctcagtcgt aaccaaagat attttacact caggaattta tgctggcaat
    19321 cccgcccgca aaatcaggga tttataatca aagaggttct atatgattcc ttttatggat
    19381 ctagaaacgc aatatcttaa tattcgagag gaaatagatt cagccatttt caacgttatt
    19441 catagttaca aattcattaa tggtcctgag gtagccacct tcgaaaagaa ttttgctaca
    19501 gcccagaacc tgaaatatat gctaggctta gcgagtggaa cagcggctct gcatctggcg
    19561 tatgaaattc ttggcataac ttccggcgat gaagtgattg ttcctgctat gactttcatt
    19621 gctacggctg aaactgtgcg tcaggtgggc gctaagcctg tttttgccga tattgatccg
    19681 gtttcgttta acattgattg caacggtcta gaaaagctga taacgcctaa gactaaagcc
    19741 attgttgtgg ttcatctgca cggtaatccc tgcgaaatgg accgaattgt cagctttgcc
    19801 aagaaatata atttgaaaat catcgaagat tgcgctcaag cccatctggc agaatttcaa
    19861 ggtcagaaag tcggtacttt cggcgatgta gccgctttta gcttttaccc tggaaaaaat
    19921 ttaggtgctt atggtgatgc cggagcgttg ggtactaacg atgaaactct ttacgaaaag
    19981 gcgaagctcc tggctaatca tgggcgcaaa gaaaaatacg tccaccagac tgaaggatat
    20041 aactaccgac tcgatacttt gcaggcggct attctggatg tcaaactccg ccacctgcca
    20101 gaatggactg agcaacgaat caacattgcc aatacctacc attcgttatt aaaaaatctg
    20161 ccaatcacct taccggaaat gaacccggcc aaaaaacatg tctatcacat ttttgctatc
    20221 gccactgatc ggcgtcaaag aataattgag gctttaaaag ccaatcaaat agctttcggt
    20281 attcattatc cattaccgct gcatttacaa ccggcttata gttatttgca ttataaacca
    20341 ggcgatttac caaattccga actgctatcc acccagtttc tatccttgcc aatgtatgct
    20401 gaactgtcaa gggataatat cgagaaaata tgccaggtga tcgcgagtgt ttattaattt
    20461 tgaaagaatt gttttcgctc ggctatttcc ttattgccgg acggaatatg ataacaattt
    20521 tacgatgaac ttttagcaaa attataaatt atgtgcggca tttgcggatt agttgatccg
    20581 aatgaaaata accaccggtt gatcaccgag atgactgatt tattaaagca tcgcggtccg
    20641 gaaaaagccg ggtacttcca cgacggtaat gtcagtctcg gacaccgacg actggcaatc
    20701 attgatttag aaactggcga ccagccgatt tataatgaag accgcacggt agtcgtcata
    20761 atgaatggtg agatttacaa ttttccggaa ttcaaggctg atttaatcca gcgcggccat
    20821 catttttata ccaactcgga cacagagatt ctggcgcaca gttacgagga atatgggctg
    20881 gaatttctcg cgcgtctcaa cggcatcttt gcctttgcat tatatgatat taaagacgaa
    20941 cgactcattt tggcgcgtga tcattttgga gtcaaacccc tccactattg gtttcagaac
    21001 gaggtgctgt tgtttagttc ggaatacaaa gcgattctgg tgcatcccgc tgtgcgccgt
    21061 caggtgaatt attccgctct gcactgccag gtcaatctac gttacaatca aagtgatgaa
    21121 acgttgatta aagggattta tcgcctgccg cccgccagtt atttgattta cgaaaaaggg
    21181 cagataagta tcaagaaata ttatcaattg ccggtaaatc tgaatttcaa aatgactgaa
    21241 gccgaagcca ttgacggcat tcgttttcac ttacaacaag ccgtgagacg ccagctaatc
    21301 agcgatgtgc cgctgggtgt ttatctttct ggaggaatgg attcgtcagc cttggtggcg
    21361 atgatggcgg ccgaaaaa
//
LOCUS       DAOOMU010000032        21378 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2404_length_21378_cov_17.098954, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000032 DAOOMU010000000
VERSION     DAOOMU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 21378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21378
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2404_length_21378_cov_17.098954"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..2437)
                     /locus_tag="PLI55_04600"
     CDS             complement(296..2437)
                     /locus_tag="PLI55_04600"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPB00147.1"
                     /translation="MKRTLIVIMTIAIFLLAFLGCSDEDNDSVTGANTDALPAYLPKG
                     TIHGLVRDICTNVPIKGAVLSLSYNGEVTTVKSNSAGQFSFDNVPAGQFTVINGASMA
                     TGTYTITASLVDYNKSISDTTKRYRDYYYSTVTITFTSLVPGDSLGVSGLVGSVVLDL
                     SQLNTTIVGQVVDDKMKPVESAYVILQDLSITPGVIIKQTQTDANGYYRFNNVDNGIN
                     VSIMARSSDGKFEGSLPGGLSLLCNLTYDSLRSRVDAEKIQLAWVNDVAPYVINITPQ
                     NNSDVSPSELAVIYTFSVPIKQTPYTRTDLGLGHKTILDDITVSFSGLKKTAGLLNTE
                     ISWNNEMTQLIIQPKNVVGSAKYDIDVTSALPKLEDEAGLKVVDNPEIVGDFEKLYFT
                     TAGQSVVPTAPSLTRRIVPGAYEYLDYNGGTVNLEWNSDPSARSYNLYRQIGDGSFEL
                     IDSDIFSLIVSDATGMLVTPAVNDPLSAITVKYQVCAVSKDLVEGPPSNTITISDDIS
                     PQLIGTSVINLASQRYQFNFRFSEPLVISSAEDLNNYTIQDPDTVVFTKERAAYMGYN
                     STNNDYEVLLTVSISNGEVLPRGFSVILGNEIVDLAGRSINSSANTYVYSSPPTPVLF
                     SPPDNSTNISSSQVLTWKISNGALSYRLQISTNVNFTSTIFDSDKITSTSFSLDAVSG
                     LVSGAKYYWRVQAANSVGSSAFSTTWSFTLQ"
     gene            2872..>3281
                     /locus_tag="PLI55_04605"
     CDS             2872..>3281
                     /locus_tag="PLI55_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPB00148.1"
                     /translation="MKINSIKAIIFDLDGTLVDTAGDILAAVNSTMEIYGLKPITYEQ
                     CLRYLGDGVEDLVRRAVENSISEPIDPNYLAEIVAQYKDFYSSHLTDRSRLFPGVRET
                     LAELQDYTLVVVSNKSHDFCVQILRQLDIEKYFQL"
     assembly_gap    3282..3381
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3382..4484)
                     /gene="alr"
                     /locus_tag="PLI55_04610"
     CDS             complement(<3382..4484)
                     /gene="alr"
                     /locus_tag="PLI55_04610"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPB00149.1"
                     /translation="MHFPQNTTIAEVDLNNLRYNYQNIKQKVAPAQIMAVVKANAYGH
                     GAVPIAQCLRDQGVQYFAVARLCEAMELIQSGIGGEILIFGTLYDEEASFAIAHNIRI
                     TVTRAEDFVTYDRLAGALQKVAKVHVKIDTGMGRVGLPAIQAFDLIKASRKLKNIEIE
                     GICTHFATADSRDKTFAYEQLARFNQIIKQLRQTGITSPLIHAANSGAILDLPETYFD
                     MVRPGITLYGHYPSLETSESIPLRQVMSLKTRVAQIREVPGGTSISYGRLYFTKKVTR
                     IAVLNIGYADGMPRAFSNKGQVMIKGQLFPIIGAVTMDQLMVEVGDADVKPGDEVLIW
                     GNSPDGILSATQVAAKAGMLIYELCCAVAPRIPR"
     gene            complement(4536..4997)
                     /locus_tag="PLI55_04615"
     CDS             complement(4536..4997)
                     /locus_tag="PLI55_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00150.1"
                     /translation="MRAYLAGAIEYAPDHGKVWRTEMEKFLQEEFGHSCYNPLIEEHK
                     YLTPDESRNFRGYKQTDPLRFQQIVRKLIQGDLRALETKIDYVICNWDKYAVKGGGTY
                     GEVTYAFYHNIPVYLVSEFPVIEISGWIIGCATKIFSSFNELKEYLKITYK"
     gene            complement(4994..5740)
                     /locus_tag="PLI55_04620"
     CDS             complement(4994..5740)
                     /locus_tag="PLI55_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB00151.1"
                     /translation="MIFKKIMLKNELRLDLHKILLGLFFNIIGTCLVYFALVTSLGKI
                     LPRIDYLEVDKFILSAIIGFTSGLMALGLASSYVTHYFQDSRGDYGRLPGMPISQIYG
                     SFLIRLWIILILHLLVTTLTVLILSGFFPGLGRLLLFWIFAILPLGIFIQLGMLIGLA
                     EDRRNQFYWIFLILVPLFLMSGMIIPTHFHAGFLGKLLMALPSSVLIEGGRSILTRQP
                     FELLNILYLLALNILLFIGGIFFFKRKLQN"
     gene            complement(5743..6666)
                     /locus_tag="PLI55_04625"
     CDS             complement(5743..6666)
                     /locus_tag="PLI55_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB00152.1"
                     /translation="MEASITLRNVSKFFKKKYVLSNLTLGIEKNTFFAIIGRNGAGKS
                     ILLRVISTFMAPDAGEVFINGEDIAKSPQMAKNCIGYLPDHDIHNRWITGWENLKARA
                     ELLEIPQSRFKEICEPLIKDFNLQEVLDEYPVTYSRGAKRCLDLVLVLLGDPQILILD
                     EPTLGLDYQNRASLFKYLLSVKNHKTIIVASNEFIEVQTLAERWIVLDYGKIRFDGTL
                     EKMLTRVDVPFSGEIILRKKDETLLEKIKESDNFSKVYDLDLTIQLTFERLQDFYAVL
                     ATIPAENLVGVNIQSLSIAEFINRLLSDEEF"
     gene            complement(6666..9383)
                     /gene="polA"
                     /locus_tag="PLI55_04630"
     CDS             complement(6666..9383)
                     /gene="polA"
                     /locus_tag="PLI55_04630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPB00153.1"
                     /translation="MIKIFHIFMEKKETLFLIDGSALAYRSYFGTIRTHLATSRGKPT
                     GAIFAFVNSLKRILEEVKPDYLAIVFDAPEKTFRHQIYTDYKATREAMPEDLVAQLPV
                     MFEIVKAMNIPMLTVPGYEADDVIGTLAKAAKNKGLQVFVVTGDKDFMQLVDDDILVY
                     KPASGQKEVEILDKNAVVSKLGVQPDQITDYLGLVGDPSDNIPGIKGIGPAKAQPLLA
                     KFGSLEKVIEQAESIGNPRLTETIKNGAEIARLSKQLATIKTDVPLDINIESLKIKPV
                     NQAELERIYRELEFYSLIETKPEVSDAVKPDKHYQTIQTQAEVRELVKNLLNKELLSV
                     DLETTSINPMMAEIVGVALCWEAHSGVYLPIRHPAMADQLFAQTDNSAWLAELKPLLD
                     NEQIVKCGQNLKYDMLILRRNGIELRGKIFDTMVAAFLIQPDARSYKLDKLSQYYLNY
                     KMQSIEELIGNGKDQLNMEQVDIDKVTFYAVEDADVVLQLVPIFEEKLKADQLWQVFE
                     EIEMPLVPVLMQLELNGVYLDVKSLKEMSRNLEVQIDAIMRRIYDLAGVEFNINSPKQ
                     LGEILFEKLNLPKVRGNSTDVSVLEKLKHKHPLPSAVLEYRGLVKLKNTYLDTLPLLV
                     NQKTGRIHSSFNQTMAATGRLSSSDPNFQNIPIRTDIGREIRRAFIPQQAGWKILSAD
                     YSQIELRIMAHLSGDAELRRAFQEGVDVHARTAALVYGVPEKDVLPEMRRIAKVVNFG
                     IMYGAGPFRMSEELGISRSDANKLIEQYFRTYPGINDYITKTIAEARQNTYVKTLTGR
                     RRYVLDINSENKNVREAAERVAINMPIQGTAADMIKIAMIRIQRQLTKEKFKSMMILQ
                     IHDELLFEVHPDELERLRQIVTTEMENALPLDVPVKVDIGVGNSWYEAH"
     gene            complement(9586..10341)
                     /gene="lgt"
                     /locus_tag="PLI55_04635"
     CDS             complement(9586..10341)
                     /gene="lgt"
                     /locus_tag="PLI55_04635"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPB00154.1"
                     /translation="MYPELLHIGPVTIYTYGLMMAIAFIVCYLIFRAETKKLGDNPDF
                     ASNVVFWAAIGGIIGAKVFYLLENIPDLLADPKGMIFSGSGLVFHGGLIGGTIAVILV
                     LRRSQKSIGVYADIIGPLLLIGQGIGRIGCFFAGCCHGKACDLPWAVTFPNASPPANY
                     PVHPTQLYEAFLNFAFYFILVKVIRPKTTKVGQTMAIYLMFAGLERFLIEFIRVNPRV
                     WGNLTSAQFTSIGLFCAGLIILLFFAKTPKKSV"
     gene            complement(10345..10947)
                     /locus_tag="PLI55_04640"
     CDS             complement(10345..10947)
                     /locus_tag="PLI55_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB00155.1"
                     /translation="MASASDFINIIKTRRTIRQFRQEQVDQAVIIEWVDCARLAPSAR
                     NLQPLEYFLITEPKLRERVFPLLRWASYIAPLGDPKEGQHPTGYLVVLINKDLMQSHY
                     AKDAGAAIENFILAAWANGVGTCWLASVEREKLRALLAIPEQYEIDSVVAFGYPAESP
                     VIENRNEIRYWLDEKNTLHVPKRPLASLLHINSWNRHEVE"
     gene            complement(10966..11421)
                     /locus_tag="PLI55_04645"
     CDS             complement(10966..11421)
                     /locus_tag="PLI55_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB00156.1"
                     /translation="MNIITTLRVSDLTHGLTDAELEALAGLFRCRHIKRDEIIFREGE
                     PSEELYIIAKGRVSVLIYSVSLPGKCEKIATLRDNEIFGEFAFIDGSPRSATVVAEED
                     TDLIFVDNVALFRFLDENEHIGYLIMRNIAKILTGKLRQTDLEMRNAIL"
     gene            complement(11444..12325)
                     /locus_tag="PLI55_04650"
     CDS             complement(11444..12325)
                     /locus_tag="PLI55_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPB00157.1"
                     /translation="MSNPKLINGVEIAQKVKDDLRPRINRLKQKGITPGLAVILVGDN
                     PASQSYVNMKAKAFESMQLFSETIRLPGESSQEKILDLVNQLNHDSRFHGILVQLPVP
                     KHLDSNAIIQAISADKDADGIHPTNLGKMVLGIEAPLPCTPHGILMLLKYSDIDPAGK
                     HVVITGRSNIVGKPIANLLMQKRPLGNATVTVCHTHTRNLPEITRLADILIAALGQPE
                     FIDRSFVKPGVVVIDVGSNRIEDASSPKGYRWVGDVKFSEVAEIASAITPVPGGVGPM
                     TIAMLIANTVLLAEKTS"
     gene            complement(12322..>13359)
                     /gene="rny"
                     /locus_tag="PLI55_04655"
     CDS             complement(12322..>13359)
                     /gene="rny"
                     /locus_tag="PLI55_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPB00158.1"
                     /translation="KEIRENALAEANRDAKKIIVSAIQRSAADHTAETTVSVVNLPND
                     NMKGRIIGREGRNIRHFESLTGVEIIIDDTPEAVVLSGFDPIRREVARIALEKLIADG
                     RIHPARIEEMINKAEKEVDEEILRAAEEAIDEVGISQFPPELLKMLGKLKYRTSYGQN
                     VLKHSIEVAWLSGLMAAELGLNGELAKRAGLLHDIGKAIDRNVTGTHALIGADFVKKF
                     RENPIVINAIASHHEEVEATHPISAIVQAADQISGSRRGARGDTLESYIQRLEKLESI
                     ATSFAGVTKTYAIQAGREVRVIVENGVVSDAEADNLAEEIAAKIQQELNYPGQVKVVV
                     VREHRSVSYAK"
     assembly_gap    13360..13459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13666..13971)
                     /locus_tag="PLI55_04660"
     CDS             complement(13666..13971)
                     /locus_tag="PLI55_04660"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPB00159.1"
                     /translation="MEESNVNIVRVTIYGQEYSVKSRADRDYIISVANYVNEKMEEVE
                     STLRTIQPSIRVAILAAMNITDELFTANQEKDKIVSSVEEKAGFLIDIIDEQLKEEI"
     gene            complement(14021..14221)
                     /locus_tag="PLI55_04665"
     CDS             complement(14021..14221)
                     /locus_tag="PLI55_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00160.1"
                     /translation="MNIVNLNLLEEKIKSLLLLVTRLKEENEKLKSELKEGASSEVML
                     DLKKRQELRAKIEGMLELLKDY"
     gene            complement(14232..16619)
                     /gene="pheT"
                     /locus_tag="PLI55_04670"
     CDS             complement(14232..16619)
                     /gene="pheT"
                     /locus_tag="PLI55_04670"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPB00161.1"
                     /translation="MIVNTNWLKKYTDFPFSPAELAERLTAIGLEANLIEDKSTGLEN
                     IIVAEILAVRPHPNADRLTICTVSTGEKQLEVVCGAPNVKQGQKVPLALVETTLPNGM
                     TLKPTKIRGVISEGMLCAEDELGLSNDHSGIMILEQDATVGSRLSDYLATDGKSLEID
                     LTPNRPDCASHIGVAREITLMTGNELRKPAINLKEIQQPTSDSIEIEIADPVGCPRYA
                     ARLIRGVKIGPSPDWLVKSLESIGQRSINNVVDAANYVLMETGHPLHTFDYAHLKGQK
                     IVVRRAKPGEKVVTLDGVERELSGDVLLICDSEKPVAIAGIMGLANSEINDDTRDVLL
                     ESAYFNPGVIRKGARFLGMQTEASYRFERGTDYEGLLYALNRLAALIVEVAGGQICRG
                     IVDNYPNPIKARRVVLRYERTNRLIGMNFEPDWIKKVLTGLGCEIINSAESEITVTVP
                     TWRPDLEREVDLIEEIVRVYGMSKVPSAKYIQIVPMETINERYDLIEKLRFFVAGCGY
                     SELYCNSLVSARAAEFGAESGDKLKLRNPLGQDMTYLRTSLLPGMIAAVKHNFNHYRT
                     DLRLFELGFVQAFAPQAETLAEESLHLGVILTGYLDEPNWAYPRRASNIFFLKGLVEA
                     IAKQFGLQPINFQKAANPYYKHLIQILTGEAKLGYLGELAPGYLDKEWDIQLPVNSME
                     LNVEVILKSLNLTKTYHEPPAFPFTERDISIIVDRTIPVDKVIENIRQKGGNLLREIR
                     FYDLYLGKNIDKKEKSLTFNLLFQANDRTLTDAEVDQIMATIYANLSRELNARLR"
     gene            complement(16624..17640)
                     /gene="pheS"
                     /locus_tag="PLI55_04675"
     CDS             complement(16624..17640)
                     /gene="pheS"
                     /locus_tag="PLI55_04675"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPB00162.1"
                     /translation="MDILNRADEVRGQLQKDLQQVSADPQAVENLRIKYLGKKGLITA
                     LFTDFNSITAEQKRQVGKILNILKKEAEAQIQALNERLKEQVAQDEYFDYSLPGLAPA
                     VGHLHPITQTLNQVISIFREVGFSVEFGPEVETDYYNFQALNFAENHPARDMQDTFFI
                     DEKRLLRTHTSPVQIRVMLKQKPPIRILAPGRVYRNEAINPRSHCVFHQVEGLYVDEN
                     VTFSELKTTLEMFAKRFFGSEVKTRFRPSFFPFTEPSAEMDVSCFICAGKGCRLCKNT
                     GWLEILGCGMVDPNVFVNVGIDPEKYTGYAFGMGIDRTALLKYGINDIRLYFEGDMRF
                     LEQF"
     gene            complement(17645..17992)
                     /gene="rplT"
                     /locus_tag="PLI55_04680"
     CDS             complement(17645..17992)
                     /gene="rplT"
                     /locus_tag="PLI55_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPB00163.1"
                     /translation="MPRANSSVPHHRKHRKIVGMAKGYRGARSKNYVSAKDAVEKALQ
                     YAYRDRRNKKRDFRSLWIARINAAANQHDISYNKFIHGLKKAGITLDRKALAEIAMHD
                     ADGFAILVALVKG"
     gene            complement(18003..18197)
                     /gene="rpmI"
                     /locus_tag="PLI55_04685"
     CDS             complement(18003..18197)
                     /gene="rpmI"
                     /locus_tag="PLI55_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPB00164.1"
                     /translation="MPKMKTRRSAAKRFTLTGSGKVKRNKAYHSHILTKKSSKRKRNL
                     RQSEILKSADVKRVKKMLGV"
     gene            complement(18209..18715)
                     /gene="infC"
                     /locus_tag="PLI55_04690"
     CDS             complement(18209..18715)
                     /gene="infC"
                     /locus_tag="PLI55_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPB00165.1"
                     /translation="MEKEVRVNEQIRSQTVRLIDFDGKQIGIVSNKEALRISEDRGLD
                     LVEVSPNTNPPVCKIMDFGKYRYQQQMKERESKKKQHVVKIKEVRFRPHIGEHDFEMK
                     INAARRFLGEGYKVKITLMFRGREMAHQETGKELVQKIIEKLADVGTPEKNPVQEGRT
                     IVTYFTAK"
     gene            complement(18712..20655)
                     /gene="thrS"
                     /locus_tag="PLI55_04695"
     CDS             complement(18712..20655)
                     /gene="thrS"
                     /locus_tag="PLI55_04695"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPB00166.1"
                     /translation="MESNRKITIHLPDNSTMEVSAGVSAGQIAKEISPGLFQNALAAK
                     VNDRLVDLSTIISEDARLEILTYNSPESQEILLHSTSHVMAQAVKQLYPQAKVTIGPA
                     IENRFYYDFDIEPPLTDGDLPAIEARMNEIIAADLPIIRREVSREEAIELFSKMGETY
                     KVEIIREIEPDVPLSIYTQGDFTDLCRGPHIPSTGKIKAFKLLNVAGAYWRGDSRNKM
                     LTRIYGTSFATQKELKKYLNFIEEAKRRDHRKLGKSLELFEINEQVGPGLVLWYPRGA
                     LLLQNIKDYWIKEHLKRGYDLVQTPHIGKAQLWETSGHLDFYRESMFDGMTVEGETYY
                     IKPMNCPFHMQIYKSKTRSYRDLPIRYAELGTVYRYELSGVLHGLMRVRGFTQDDAHI
                     ICTPEQLNDEIQSLVLFSFEFVRSFGFKEMDVYVATRPKEKYVGSPENWAEATEALKA
                     ALSKLDIEYHIDEGGGAFYGPKIDIKIRDALNRSWQCTTIQFDFNEPERFNMDYIASD
                     GNKRRPYMIHRAIMGSLERFAGVLIEHTVGDFPLWIAPVQAVVIPVTNNQDQIATEFY
                     KLLIDNGIRAEINLQNDTIGAKIRDAELMKIPFMFIIGEREATGRQISIRRRKIGDTG
                     VMSWEEAIDLIVKEIQLKKENKS"
     gene            complement(20838..20914)
                     /locus_tag="PLI55_04700"
     tRNA            complement(20838..20914)
                     /locus_tag="PLI55_04700"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20878..20880),aa:Val,seq:gac)
BASE COUNT         6035 a         4688 c         4208 g         6247 t
ORIGIN      
        1 attctacaat acttcaaccg ctccgcggtg attgtttatc tgaaaaccat agattcccaa
       61 ctagcccgta gggctttaag ttttgtagaa aaacataatc gccagaaatt gctacctcag
      121 agaggtttaa caaatttcga atatcaattt taattataat tagccatcgt ttcttgctaa
      181 ataacggcaa ttcaaaatcg ggttagatct tcttgtaaac cttatttctc tactattatc
      241 acgaaaaagg gcggattata taaacccgcc cttttatctt gatcaaatag tgaatttact
      301 gtaaggtaaa actccaagta gtagaaaatg cactacttcc gaccgagtta gccgcttgta
      361 cccgccagta gtatttcgca ccgcttacca accctgatac tgcatctaag ctaaaagagg
      421 ttgacgtaat cttatcacta tcaaatatcg ttgaggtaaa attgacatta gtcgaaatct
      481 gaagacgata agataatgca ccattcgata ttttccacgt caatacctga gaggatgaaa
      541 tattagtcga gttatcagga ggtgaaaaca aaactggggt tggtggagat gaataaacat
      601 acgtattggc agagctatta atacttcttc cggcaagatc tactatctcg ttgccaagta
      661 ttactgagaa tccacgaggc aaaacctcac cgttggaaat tgaaaccgtt aataatactt
      721 cataatcatt attagtagaa ttataaccca tataggcggc tcgttccttg gtgaatacta
      781 ctgtgtcagg atcctgaatt gtataattat ttagatcttc ggcagagctg attaccagcg
      841 gttctgaaaa acgaaaattg aattgatatc gttgagaagc aagattgatc accgatgttc
      901 caattagttg cgggctaata tcatcactaa tcgtaatggt attactcgga ggtccttcta
      961 ctaaatcttt gcttaccgca caaacctgat atttaaccgt aatcgccgag agtggatcgt
     1021 ttaccgcagg agttacaagc atacccgtcg catcactgac tataagtgaa aaaatatcag
     1081 aatcaatcaa ctcaaacgaa ccatccccga tttggcgata taaattataa cttcgcgctg
     1141 atggatccga attccattcc aaattcacag tgccaccgtt ataatccaaa tattcataag
     1201 ctccgggaac tatgcgacgc gtgagactcg gtgcggttgg aactactgac tgaccagcag
     1261 tcgtaaaata caacttctca aaatcgccca cgatttctgg attatctacg acctttaaac
     1321 cagcctcatc ttccaacttt ggtaaagcag aagtaacatc aatatcgtat ttagccgaac
     1381 caacaacgtt tttcggttgg atgataagct gggtcatctc attattccaa gaaatttcag
     1441 tatttaacag cccggctgtc ttcttgagtc cggaaaacga aactgtaata tcatcaagaa
     1501 ttgttttatg accgagacca agatccgttc gtgtataggg cgtttgttta attggaactg
     1561 agaaggtgta aataaccgct aactcggaag gagaaacatc ggaattattt tgaggtgtaa
     1621 tattaataac ataaggggct acatcattta cccaagcgag ttgaattttc tcggcatcca
     1681 cgcgtgagcg caaagaatca taagtcaggt tacatagtag cgataatcca cctggcaaac
     1741 taccttcaaa cttcccatcc gaactacgcg ccataataga aacatttatc ccattatcaa
     1801 cattattaaa ccgatagtag ccgtttgcat cggtttgggt ttgctttatt attacccctg
     1861 gtgtaattga aagatcctgt aaaataacgt acgctgattc caccggtttc attttgtcat
     1921 ccaccacttg cccgacgatt gtggtattta actgggatag gtctaaaaca acactaccga
     1981 ccaaaccact gacacccaga gaatcacccg gaaccaggga agtaaaagta atcgtaacgg
     2041 tactatagta ataatctcga tagcgtttgg tcgtatcact tattgattta ttgtaatcaa
     2101 ctaatgatgc ggtgatagtg taggtgcccg ttgccatgga ggcaccgttt ataacggtga
     2161 attgcccagc agggacatta tcaaaggaaa actgcccggc gctattggat ttaaccgttg
     2221 ttacctcacc attatacgat aatgaaagaa ctgccccttt tatcggcaca ttcgtacata
     2281 tatctctgac caaaccgtgg atagtacctt ttggtaggta cgcgggaaga gcgtcagtat
     2341 tggctcctgt tacagaatcg ttatcttcat cgctacagcc aagaaaagcc aacagaaaaa
     2401 tcgcaattgt cataataaca attaatgttc gtttcataca tcctcctaca attggttaat
     2461 aattttatat tgaattatcc ctttatgtga aatgtcctgg ttcatttgct atattgatta
     2521 aaatttactc catccaaatg tcttagtatg gtaaaaataa atgcgcataa attcttttta
     2581 aagtaaggat ttatttcaaa aactaaaagc aaaatttcaa ataaggagct ttatcaaaat
     2641 catattgaaa catttgtgat tctatttctc tacaatacct tgctcactac gcggttagcg
     2701 ttgacatacc tatcatttat ttccgactag tctatagggc ttgaagtttt gtagtaatta
     2761 ttcgctcaaa atatttcccc acctcggaga ggtttaactt tctaaaattg agacaaattt
     2821 tgatactacg ggaggttttc gaaataccga cgttctgatt aaattccaat tatgaaaatt
     2881 aattcaatta aagcgattat ttttgatctt gatggtactc tggttgatac tgctggtgat
     2941 attttagccg cggtaaactc cacaatggaa atttatggct tgaaacccat cacttatgag
     3001 cagtgcctga ggtatttggg cgatggagtc gaagacctag tcagacgagc agtcgaaaat
     3061 tcaattagtg aaccgattga tccaaactat ctggctgaga ttgtcgccca atataaggat
     3121 ttttattcat ctcatttaac cgatcgctct cgtctatttc ccggtgtgcg cgagactctg
     3181 gctgaattac aggattatac attagtagta gtttccaata agagccatga cttttgtgtc
     3241 caaatcttaa gacaattgga catcgagaaa tacttccagc tnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn ncgcgggata cgcggagcaa cggcacaaca tagttcatag
     3421 atcagcatcc cagccttcgc ggctacttga gtagcggata atatgccatc gggtgaattg
     3481 ccccagatca aaacttcatc accaggcttg acatcggcgt cgccgacttc taccatcaat
     3541 tgatccattg tgacggctcc tattatcgga aaaagctgac ctttgatcat tacctgtcct
     3601 ttattggaaa aggctcgggg cattccatcg gcatagccga tattcaggac ggcaatacga
     3661 gttactttct ttgtaaaata aagacgtccg tagctaatgc tggttccccc tggcacttct
     3721 ctaatctggg caacccgagt cttcaaagac attacctggc ggagtgggat agattcgcta
     3781 gtttctaacg aaggataatg accataaagc gtaattccag gccgcaccat atcaaaatag
     3841 gtttccggca gatccagaat ggcgccgcta ttggcggcat gaatgagtgg ggaagttatc
     3901 ccagtttgac gtaattgctt tataatttga ttaaagcggg ctaattgttc atacgcaaag
     3961 gttttgtcac ggctatcagc agttgcaaaa tgggtacaaa taccttctat ttcaatgttt
     4021 ttcaattttc gagaggcttt gataagatca aaagcctgaa tagccggtaa tccgactcgt
     4081 cccatcccag tatcaatttt gacgtgcact ttcgcaactt tctgcaaagc gcccgccaag
     4141 cggtcatagg ttacaaaatc ttccgcacgg gttacggtta tccggatatt atgcgcgatc
     4201 gcaaaggatg cttcttcatc gtagagtgtt ccgaaaatca aaatttcccc gccgatgccc
     4261 gattggatta gttccattgc ttcgcacaga cgcgctaccg caaagtattg gacaccttga
     4321 tctcgcagac attgagcgat agggaccgcg ccgtggccat aggcattggc tttgacaacg
     4381 gccataattt gagcaggtgc aactttttgt tttatatttt gatagttata gcgaagatta
     4441 ttgaggtcta cttctgcgat ggttgtgttt tgcggaaaat gcatattcta tccaatcata
     4501 tttttttatc gagatggctt tcatcttttt aatttttatt tatatgttat ttttaaatat
     4561 tcttttaatt cattgaacga agagaaaatt ttagtggcac agccgataat ccagccactg
     4621 atttcgatta ccggaaactc tgaaactaaa taaaccggaa tattatggta aaaagcatag
     4681 gttacctcgc cataggtgcc tccacccttc acggcgtatt tatcccaatt gcaaataaca
     4741 taatctattt tggtttccaa tgcccgtaaa tcaccttgaa tcaatttccg gacaatttgc
     4801 tgaaaacgca aagggtcagt ttgtttatat ccgcggaaat tacgcgattc gtcaggtgtc
     4861 aaatatttat gttcctcaat caaaggatta taacaggagt gcccgaactc ttcctgtaaa
     4921 aacttttcca tttctgtccg ccagaccttc ccgtggtcgg gggcgtattc aatcgcaccg
     4981 gctaaatagg ctctcaattt tgcaatttcc ttttgaaaaa gaaaatccca ccaataaaaa
     5041 gcaagatatt tagcgccagc aaatataaaa tgtttaggag ctcaaatggt tgacgcgtta
     5101 aaatagatcg cccgccttcg atcaatactg aggatggcaa agccatcagc aatttaccta
     5161 aaaatcctgc gtgaaaatgg gttggaatga tcatcccgga cattaaaaat agaggtacga
     5221 gtattagaaa aatccagtaa aactgattac gccgatcttc cgctaaacca atcaacatac
     5281 ctaactggat aaatatacca agcggtaata ttgcaaatat ccagaaaagt aatagtctgc
     5341 ctaatccggg gaaaaacccc gaaagtatca gaacggttaa ggtcgtaaca agcagatgaa
     5401 gaattagaat tatccacagt cgaatcagga aactgccata aatttgtgag attggcattc
     5461 cgggaaggcg cccatagtca ccgcggctgt cctgaaaata gtgcgtcaca taggaactgg
     5521 ctaatcctaa cgccatcagt ccgctggtaa atcctatgat cgcagacaat ataaatttat
     5581 caacctcgag gtaatcaatt cgcggcagaa ttttccccaa actggttacc agagcaaaat
     5641 acactaaaca agtaccgata atattgaaaa ataagcccag caaaatttta tgcaaatcaa
     5701 ggcgtaactc attctttaac attattttct taaatatcat tactaaaact cttcatcaga
     5761 taataaacgg ttaataaatt cagcgatact tagagactgg atattcactc cgaccagatt
     5821 ttcagcggga atcgtagcca gtacggcata gaaatcttgg agtctttcaa aagtcagctg
     5881 gatggttaaa tccagatcat agactttaga aaaattatcc gattccttaa ttttttccag
     5941 taaagtctca tcttttttgc gcaagataat ttccccggaa aatggcacat ctactcgggt
     6001 taacattttt tcgagggtgc cgtcgaatct aattttccca taatccagaa caatccagcg
     6061 ttcagcaaga gtttgaactt ctataaattc atttgacgcg acgataatcg tcttgtggtt
     6121 tttaacggag agtaagtatt taaataacga tgcgcggttc tggtaatcca gacctaaagt
     6181 tggttcgtct aaaattaata tctgcgggtc tccgagcaaa accagaacaa ggtcaagaca
     6241 gcgcttagcg ccgcgtgaat aagtcaccgg atattcatcc agcacttctt gtaaattgaa
     6301 gtctttgata agaggctcac aaatctcttt gaaacgagac tgaggtattt ccagtagttc
     6361 agccctggct ttgagatttt cccagccggt aatccagcgg ttatgaatat cgtgatcggg
     6421 taagtaacct atacaatttt tagccatttg cggggatttg gcaatatctt cgccgttgat
     6481 aaaaacttct cccgcatccg gtgccataaa agttgatatt actctcaaaa gaatagactt
     6541 tccagctcca ttcctgccaa ttatagcgaa gaatgtattt ttttcgatgc cgagagtcag
     6601 attactcaga acatatttct tcttgaaaaa tttgcttaca tttctaagcg taatagaagc
     6661 ttccattaat gcgcctcata ccatgaatta ccaactccaa tatctacctt aaccggaaca
     6721 tccagaggta aagcgttttc catttcggta gtgactattt gacgaagacg ttccaattca
     6781 tccggatgaa cttcaaataa aagttcatcg tgaatctgga gtatcatcat tgatttgaat
     6841 ttttctttag ttaattgacg ctgaattcta atcatcgcga ttttgatcat atcggcggct
     6901 gtgccttgaa tcggcatatt gattgccacg cgttcagcgg cttcccgcac atttttattt
     6961 tcactattaa tatcgagaac atagcgtcgc cgaccggtta aggtttttac gtaagtattt
     7021 tgccgggctt cggcgattgt ttttgttata taatcattaa tgccgggata agtgcggaaa
     7081 tattgttcaa tgagcttatt cgcatcggaa cgtgaaatcc cgagctcttc cgacattcga
     7141 aatggaccgg cgccgtacat aatgccgaag ttaacgactt tggctatcct gcgcatttcc
     7201 ggcaagacat ctttttcggg aacgccataa accagggctg ccgtgcgggc atgcacatct
     7261 accccttcct gaaatgcgcg tctgagttcg gcatcaccgg ataaatgcgc cattatccgc
     7321 aattcaattt gagaataatc agccgataaa atcttccagc cagcttgctg aggtatgaat
     7381 gcccgacgaa tctcgcgacc aatgtcggtg cgaatcggaa tattctggaa attcggatca
     7441 ctgctactca accgtccggt tgcggccata gtttgattga atgaactatg gatccgccca
     7501 gttttttggt taaccagaag tggcaaagta tctaaataag tgttctttaa tttcaccaaa
     7561 ccgcgatatt caagcacggc agacggcaga ggatgtttat gctttaattt ctccagaacg
     7621 ctaacatcgg tcgaatttcc gcggactttg ggtaggttca gtttttcgaa taggatttcg
     7681 ccgagttgtt tgggagaatt gatattgaac tcgacacccg ccagatcgta tatccgccgc
     7741 ataatggcat caatttggac ttccagattg cgtgacattt ccttcaaact tttcacatct
     7801 agataaacgc cgtttaattc cagttgcatt aaaaccggaa cgagaggcat ttcgatctct
     7861 tcaaaaactt gccagagttg atcagccttt agcttctctt cgaatatcgg taccagttgt
     7921 aaaacaacat cggcgtcttc gacggcatag aatgtcactt tatcaatatc aacctgctcc
     7981 atattcaatt gatctttgcc gtttccgatc aattcttcga ttgactgcat tttataattc
     8041 aaataatact gactcaattt atcgagtttg taagagcgag catcgggttg aatcaggaaa
     8101 gcggctacca tagtatcgaa gatcttgccg cgcaattcaa tgccgttgcg gcgcaaaatc
     8161 agcatatcgt atttcagatt ctgcccacat ttaacgattt gctcattatc taaaagcggc
     8221 ttaagctccg ctaaccatgc tgaattatcg gtttgagcga aaagctggtc agccattgcc
     8281 ggatgtctga tcggcaaata aaccccggag tgggcttccc agcacagcgc aactccgacg
     8341 atttcagcca tcattggatt aatagaagtc gtttccagat caaccgataa taattctttg
     8401 ttaagtaagt tttttaccaa ctcgcgcact tcagcctgag tctggatggt ttggtaatgt
     8461 ttatccggtt tgacggcatc tgagacttcg ggtttcgttt cgattaacga gtagaattca
     8521 agttcgcgat aaatacgctc cagttccgcc tggttgaccg gtttaatttt taacgactca
     8581 atattaatat ccagcggaac atcagtttta atcgtggcta attgtttaga aagccgggca
     8641 atctccgccc cattttttat cgtttctgtt aggcgagggt tgccgattga ttcagcctgt
     8701 tcgatcactt tttcaaggga gccaaattta gccaaaagtg gttgtgcctt agcaggacca
     8761 atgcccttaa tccctggaat attgtcggaa ggatcgccga ccagaccgag gtaatcagta
     8821 atttggtcgg gctgaacacc taatttgctg actaccgcat ttttatcgag aatctcaacc
     8881 tctttctgtc cggaagccgg tttatagacc aaaatatcat catccaccaa ttgcataaaa
     8941 tctttatcac cagtaaccac gaaaacctgc aagcctttgt ttttggcggc tttggcaagc
     9001 gtaccaatca cgtcatcagc ttcataaccg ggtactgtga gcatcggaat attcatcgcc
     9061 ttaacgattt caaacataac cggcaattga gccactaagt cttccggcat cgcttcgcga
     9121 gtagctttat aatcggtata aatctgatgg cggaacgtct tctccggggc atcgaaaact
     9181 atagccagat aatcgggttt gacttcctcg aggatgcgtt tcaatgaatt aacgaaagcg
     9241 aatatcgcgc cagtcggttt ccctcgggaa gtcgccaaat gggtgcgaat ggtcccgaag
     9301 taagaacggt acgctaaagc cgacccatct attaaaaata atgtttcctt cttctccata
     9361 aaaatatgga aaattttaat cattaactgg caatttgcca aacgatttca gtgaaaagta
     9421 gagcaagtgt tgttaagtgc tgataagtgt ttttagtgtt attaagaaaa ttgttgtcaa
     9481 aaaaatagtt ttaaggcttg aaggtatatt taaaacggaa tattacggaa atatctcaat
     9541 attttaaaag cacttaagag aatttaagcg gacttaaaag tacttttaaa cacttttctt
     9601 aggagttttg gcaaagaata ggagaattat taatccggca cagaataatc caatagaagt
     9661 aaactgggcg ctggtgagat tcccccaaac gcgcggattg actcggataa attcaatcag
     9721 gaagcgttcc agtccggcga acatcaagta gattgccata gtttgcccga ctttggttgt
     9781 tttggggcga attactttta ccaagataaa ataaaaagca aagttcaaga aagcctcata
     9841 aagctgagtc ggatgcaccg gataattagc cggcggtgaa gcattaggaa aagttactgc
     9901 ccagggtaaa tcacaggctt taccatggca acaaccggca aaaaaacagc cgatacgccc
     9961 tataccctgc ccgattaaaa gcaatggacc aatgatgtca gcgtaaacgc caatactttt
    10021 ttgcgagcgg cgtaaaacca gaatcacggc aattgtccca ccgatcaatc caccgtgaaa
    10081 tacaagcccg gaaccggaaa agatcatccc tttcggatca gctaataaat ccggaatatt
    10141 ttccagtaga taaaatactt ttgccccgat tattccaccg attgccgccc aaaatacaac
    10201 attactggcg aaatcaggat tatcgccgag ttttttagtc tcggctcgga atatcaaata
    10261 gcaaacaata aaagcgatcg ccatcattag accataagta taaatagtca ccggaccgat
    10321 atgcagaagt tcaggataca tatttcattc cacctcgtgt ctattccaac tgtttatgtg
    10381 taataaactg gctaatggtc tcttgggaac atggagagta tttttctcat ccagccaata
    10441 gcgaatttca ttcctatttt ctatcacagg cgattcagcc ggatagccaa aggcaaccac
    10501 cgagtcaatt tcatattgct cgggaattgc gagcaaagcc cgcaattttt cgcgttcgac
    10561 tgaagcaagc cagcaagtgc caactccatt tgcccaagcc gccagaatga aattttctat
    10621 cgccgcgccg gcatctttag cataatgcga ttgcattaaa tctttattta tcaaaaccac
    10681 caaataaccg gtcggatgtt gtccctcttt gggatcaccc aatggtgcaa tgtaactcgc
    10741 ccagcgtaaa agtggaaaaa ctcgctccct taatttcggc tcggtaatca gaaaatactc
    10801 aagtggttgg agattgcgag cggacggtgc taagcgggcg caatccaccc attcgataat
    10861 aacggcttga tcaacttgtt cctgacgaaa ctggcggatt gtacggcgag ttttaataat
    10921 attgataaaa tcagaagctg aagccataaa cagacccttt cggatttaaa gaatagcgtt
    10981 ccgcatttcg agatcggttt gacgtaattt gccggtaaga attttagcga tgttgcgcat
    11041 aattaagtag cctatatgct cattttcatc taaaaaccga aataaagcga cattatctac
    11101 aaaaatgaga tcggtatctt cttcagccac aactgttgct gagcgtggtg agccgtcaat
    11161 aaacgcaaat tctccgaaaa tttcattatc acgcagggtg gcaatttttt cgcatttgcc
    11221 gggaagtgat accgaataaa tcaaaacact cacccgccct tttgctataa tatataattc
    11281 ttcactgggt tcaccttctc tgaaaattat ttcgtctcgt ttaatgtgac gacagcgaaa
    11341 caaaccggct aaagcctcaa gttcagcatc tgtcaaccca tgtgtcaaat ccgaaactcg
    11401 cagagtggta attatattca tcatccctcc tgtgttatta attttatgat gtcttttctg
    11461 ccaataaaac agtgttggca atcagcatag caatcgtcat cggaccgacc ccgcccggta
    11521 ccggcgtaat agcggaggca atttcggcta cttcactgaa tttgacatcg ccgacccagc
    11581 gatagccttt tggtgatgat gcatcttcaa tccgattgga tccgacatca atcaccacca
    11641 ctccgggttt tacaaatgaa cgatcaataa actccggctg gcccagcgcg gcaatcaaaa
    11701 tatcagccag acgcgtaatt tctggcagat tcctagtgtg cgtatgacaa accgtaactg
    11761 tggcattacc gagcggtcgt ttctgcatca ataaattagc aatcggcttg ccaacgatgt
    11821 tactccgacc agttataact acatgtttac cggctgggtc tatatcggaa tatttcaaaa
    11881 gcattaaaat tccatgcggc gtgcagggca acggcgcttc aatccccaaa accattttcc
    11941 ccaaattggt gggatgaatg ccatccgcgt ctttatcggc tgaaatagcc tgaataatgg
    12001 cattactgtc cagatgcttt ggcaccggta attgcaccag aatcccgtgg aatcgcgaat
    12061 cgtggtttaa ttgatttacc aagtccagaa ttttctcctg cgaactttcg cccggcagac
    12121 gaatagtttc agaaaaaagt tgcatactct cgaaggcttt ggctttcata ttgacataag
    12181 actgggaagc cggattatcg ccgactaata tgaccgctaa gcccggcgtg attcctttct
    12241 gtttaagacg atttatacgc ggtctgagat cgtctttaac tttttgggcg atttcaacgc
    12301 cgttaatcaa ttttggatta ctcatttggc ataactcaca ctacgatgtt cacgaacaac
    12361 gacaactttt acctgaccgg ggtaattgag ttcctgctgg attttggcgg cgatttcttc
    12421 tgccagatta tcggcttctg catcgctaac caccccattt tcgacgatga cacgtacttc
    12481 gcgtccggct tgaatagcgt aagtcttcgt aaccccggca aaggaagtcg cgatggattc
    12541 cagcttttcc aaacgctgaa tataactttc cagcgtatca ccacgtgctc cgcgccgtga
    12601 accgctaatc tggtcagcgg cttgtacaat ggcagaaatc ggatgagtcg cttcgacttc
    12661 ctcatgatga gaggcaattg cattaattac tatcggattt tctctgaatt ttttgacaaa
    12721 atccgcaccg atcaaggcgt gggtaccagt gacatttcga tcaatagctt tgccgatatc
    12781 atgcaataga ccggctcttt tggctaattc accatttaat cccaattcag ccgccattaa
    12841 gccggataac caggcgactt caatggaatg ttttaaaaca ttttgaccat aggaggtgcg
    12901 gtatttcagt ttgccgagca tttttagcaa ttccggcgga aattgagaaa tgccaacttc
    12961 atcaatagcc tcttccgccg cacgcaaaat ttcctcatca acctccttct ccgctttatt
    13021 aatcatttct tcgatgcgcg ccgggtgaat tcgcccatcg gcaattaatt tttccagagc
    13081 aatgcgggca acttcgcgac gtattggatc aaaaccggaa aggacaacgg cttccggcgt
    13141 atcgtcaata ataatttcaa ctccggtcaa tgactcgaaa tggcggatat tgcgtccctc
    13201 gcgtccaata atacgtccct tcatattatc attcggcaaa ttgaccactg agacagtcgt
    13261 ttcggccgta tggtcagccg ccgagcgctg gatagccgag acgataattt ttttagcatc
    13321 gcgattggct tcggctaagg cattttcgcg gatttccttn nnnnnnnnnn nnnnnnnnnn
    13381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnng tcgcctgtgc ccttttcaat ttttgggtat atatgtaaag
    13501 cgataaggca gtcccaacaa ctgctccgcc tattgcgaag ataatatagg tccaggtcat
    13561 tacacttctc cttatagaat cggttagaac tctaccgtta attttagata attccaaccg
    13621 taataaggat ggtagagtta atctgattta tttatttaag atggattaaa tttcttcttt
    13681 aagttgctcg tcaatgatat caatcaagaa acctgccttc tcctcgacgg aactgactat
    13741 tttatctttt tcttgattgg ctgtaaataa ttcatcggta atattcattg cggctaaaat
    13801 agctacacgg atagagggtt gaatagtacg gagcgtagat tcgacttcct ccatcttctc
    13861 attgacatag ttggcaacgc tgataatata gtcgcgatcg gctctggact ttacactgta
    13921 ttcctgacca tagatagtta cacggactat atttacgtta gattcttcca tcttttttaa
    13981 atcaattttt gtttcaaata acgttacggt tggataggga ttaataatcc tttaaaagtt
    14041 cgagcatacc ttcgatttta gctctaagtt cctgtctttt tttaaggtct aacattactt
    14101 ccgaagaggc tccttctttt aattccgact tcaatttctc attttcttct ttaagacgag
    14161 ttacgagcaa caacaaactt ttgatttttt cttctaataa attcaaattg acgatgttca
    14221 ttttgctctt ctcatcttaa acgggcattc aattcccgtg ataaattggc ataaatagtt
    14281 gccataattt gatcaacttc ggcatcggtt aaggttcgat cattagcttg aaataacagg
    14341 ttaaatgtaa gacttttttc ctttttgtca atattttttc caaggtatag atcatagaac
    14401 ctgatttcac ggagtaaatt tccacctttt tgtctaatat tttcaatgac tttatccacg
    14461 ggaatcgtgc gatctactat gattgaaata tcgcgttcgg taaacggaaa agctggcggc
    14521 tcgtgatacg ttttagtcaa attcaaactt ttcaggatga cttccacatt taattccata
    14581 ctgtttaccg gtaactgaat gtcccattcc ttatcaaggt aaccgggggc taattccccc
    14641 aaataaccca atttagcctc accagtcagg atttgaatta aatgcttgta ataaggattt
    14701 gcggcttttt gaaaattgat aggctgaaga ccaaattgtt tggcaatggc ttctaccagt
    14761 ccttttaaaa agaaaatatt actggcgcgg cgtggatatg cccaatttgg ttcatctaaa
    14821 tatcccgtta aaatcactcc cagatggagt gattcctcag ccagagtctc ggcttgcggt
    14881 gcaaaagcct gtacaaaacc cagttcgaaa agtcttagat cggtgcgata gtgattgaaa
    14941 ttatgtttaa ccgccgcaat cataccaggt aataatgagg ttcgcagata agtcatatcc
    15001 tgaccgagtg gattacgcag ttttaactta tcaccagatt cagctccaaa ttcagccgct
    15061 ctggcactaa ccaaagaatt gcaatacaat tcggaatagc cacaaccggc tacgaaaaac
    15121 cgcaactttt caatcaaatc gtagcgctcg ttgatagttt ccattggaac aatctggata
    15181 tatttagccg acggtacttt gctcattcca taaacgcgga caatttcctc gatcaggtca
    15241 acttctcgct cgagatcagg ccgccaggtc gggacagtaa ccgtgatttc actctcagcg
    15301 ctattaataa tttcgcaacc taatccagtc agtacttttt tgatccaatc cggttcaaaa
    15361 ttcatcccta tcaaacgatt agtacgttca taacgcaaaa caactctgcg agctttaatc
    15421 ggattgggat aattatccac gatacctcgg caaatctgac cgcccgctac ttctacaatt
    15481 agggccgcca gtcggttcag cgcatagagg agaccttcat aatcggtgcc gcgttcaaag
    15541 cgataagacg cttcggtttg catacccaga aaacgcgctc cttttcgaat cacgcctgga
    15601 ttgaaataag cactttccag caagacatcg cgagtatcat cattgatctc gctattcgcc
    15661 aaacccataa tcccggcaat tgctaccggc ttttcgctat cgcaaattaa gagaacatcg
    15721 cccgaaagtt cgcgttcaac tccatcaagc gtaacgactt tttcgcccgg tttagcccga
    15781 cgtacaacaa ttttctgccc tttgagatgg gcataatcga aagtatggag cgggtgtccg
    15841 gtttccatta agacataatt ggcggcatct accacattat tgatggaacg ctgtccaata
    15901 ctttctaaac ttttaactaa ccaatccggt gagggaccga tttttacacc gcgaattagg
    15961 cgggcggcat agcgcgggca accaaccgga tcggcgattt cgatttcgat ggaatcgcta
    16021 gttggttgct ggatttcttt gagatttatg gctggcttgc gtagttcatt gccggtcatc
    16081 aatgtgattt cacgagcgac tccaatatgc gatgcgcagt cagggcgatt gggagtcagg
    16141 tcaatttcca gactctttcc atcagtcgcc aaataatcag aaaggcgact accaaccgtc
    16201 gcatcttgtt ccaggatcat aatcccacta tgatcattgg aaagccccag ttcatcttcc
    16261 gcacaaagca taccttcgga gattacacct ctgattttag ttggtttaag cgtcatacca
    16321 ttcggtaatg tggtttccac cagtgccagc gggacttttt gcccttgttt gacattaggc
    16381 gcgccacaga ctacctcgag ttgtttttct ccggttgaaa cggtgcaaat cgtcagacgg
    16441 tcggcattag gatgcggtcg aacagctaat atttcagcga cgatgatatt ttctaaaccg
    16501 gtgcttttat cctcgattaa attagcttcc aagccgatgg cagttagacg ttccgctaat
    16561 tctgccgggc tgaagggaaa atcggtgtat ttttttagcc agtttgtatt gactatcata
    16621 aggttaaaac tgttccagaa agcgcatgtc accttcaaaa taaaggcgaa tgtcatttat
    16681 gccgtatttt aacaacgccg ttcggtcgat tcccatgccg aaagcataac cggtatattt
    16741 ctccgggtca atgccgacat tgacaaaaac gttcggatct accataccgc aaccgagaat
    16801 ttccaaccag ccagtatttt tacagagtcg gcaaccttta ccagcacaaa taaaacagga
    16861 aacatccatt tcagcacttg gttcagtgaa cgggaaaaaa ctgggacgaa aacgagtttt
    16921 tacctcgcta ccgaaaaagc gtttcgcaaa catttcgagt gttgttttca attcgctgaa
    16981 ggttacattt tcatcaacgt atagtccttc aacttggtga aaaacgcaat gactgcgcgg
    17041 attgatggct tcattccgat atactcttcc cggggctaaa atccggatcg gtggtttttg
    17101 ctttaacatc acgcgaattt gaaccggtga agtatgggtt ctcagcagtc ttttctcgtc
    17161 aatgaagaag gtgtcttgca tatcgcgcgc tggatgattt tctgcaaagt ttaaagcttg
    17221 gaaattataa taatcggttt cgacctccgg cccaaattcg acactaaaac cgacttctcg
    17281 aaaaatagaa atgacctggt tgagtgtttg ggtaatcggg tgtaaatggc caacggcagg
    17341 cgccagacct ggtaaactgt aatcgaaata ttcgtcctga gcgacctgct ccttcagtcg
    17401 ttcatttaaa gcctgaatct gagcttcagc ctctttcttc aaaatattga ggattttacc
    17461 aacctggcgt ttttgctcgg cggtaatcga attgaaatcg gtaaaaagcg ccgttatcag
    17521 acctttttta cctaaatatt taattctgag attttcaacc gcttgaggat cggcgctgac
    17581 ctgctgaaga tctttttgca gttgcccccg aacctcatca gcccgattca ggatgtccat
    17641 actctcagcc ttttaccagc gctaccaata tcgcaaatcc atcggcgtcg tgcatggcaa
    17701 tttcggccaa agctttacgg tcgagagtaa tcccagcctt cttcagaccg tggataaatt
    17761 tattatagga aatgtcatgt tgattagcgg cggcattaat gcgcgcaatc cagagagagc
    17821 gaaaatcgcg ttttttattg cgacgatcgc gataggcata ttgtaaggct ttttcgacgg
    17881 catctttggc actaacgtaa tttttactgc gtgcgccacg gtagccttta gccatcccga
    17941 caattttacg atgttttcta tgatggggaa cggaactatt tgctcttggc ataattttac
    18001 tcctatactc caagcatctt tttaacgcgt ttaacgtcag cgcttttaag aatttcagat
    18061 tgtcttaaat tgcgtttgcg tttgctggat tttttagtca aaatatgaga atgataggct
    18121 ttgttacgtt taacttttcc acttccggtc aaggtaaaac gtttagccgc tgatcttctg
    18181 gttttcattt taggcatatt tttttctcct atttagccgt gaagtaagta acgatggttc
    18241 gcccttcttg gacaggattt ttttcaggtg ttccgacgtc agctagtttc tcgataattt
    18301 tttgcaccag ttcttttccg gtttcctgat gagccatctc gcgaccgcgg aacattagag
    18361 tgatcttgac tttgtaacct tcgccgagga atcgtcgggc ggcgttgatt ttcatttcaa
    18421 aatcatgttc cccgatatgc ggtcggaaac gaacttcctt aatcttaaca acatgctgtt
    18481 ttttcttact ctcacgctct ttcatttgtt gttgataacg atatttacca aaatccatta
    18541 tcttacagac gggtggatta gtgttcggtg atacttccac caaatcaagc ccacgatctt
    18601 cggatattcg tagagcttct ttattactga caatgccaat ctgtttccca tcaaagtcta
    18661 ttagacggac agtttggctt cgaatttgtt cgtttacccg tacttctttc tctatgactt
    18721 gttctccttc tttaattgga tttctttaac aattaaatca atggcttctt cccagctcat
    18781 tacgccggta tcgccgattt ttcgacgtct aatcgaaatc tgtcgtccag tggcttctct
    18841 ttctccaatt ataaacataa atggtatttt catcagctca gcatcacgta ttttagcacc
    18901 aatggtatca ttttgcaaat tgatttcagc tcgaattcca ttgtcaatta ataatttata
    18961 aaattctgtt gcgatttgat cctgattatt tgtcaccgga atgacaactg cctgaaccgg
    19021 cgcgatccat agcggaaaat ctccgacggt atgttcaatc aaaacgccag caaatctttc
    19081 cagcgaaccc ataatcgcgc ggtgaatcat ataaggccga cgcttattgc cgtcactggc
    19141 gatataatcc atattgaaac gttccggctc gttaaaatcg aattgaatag tcgtacattg
    19201 ccaggaacga ttcaaagcgt cacggatttt aatgtcaatt ttgggaccgt aaaaagcgcc
    19261 gccaccttcg tctatgtgat attcaatatc gagtttactt agtgccgctt tcaaagcctc
    19321 ggtggcttcc gcccaatttt caggcgaacc gacatatttt tctttcgggc gggtagcgac
    19381 atatacatcc atttctttaa agccaaacga gcgaacgaat tcaaatgaga agagcaccaa
    19441 actctggatt tcgtcattga gttgctccgg cgtgcaaata atatgggcat catcttgagt
    19501 aaagccccgt acgcgcatta atccgtgtaa cacgccagat aattcatagc gataaaccgt
    19561 ccccagttcg gcataacgaa tcggcaggtc gcgataacta cgcgttttcg atttataaat
    19621 ctgcatatgg aacggacaat tcattggctt gatatagtaa gtctcgcctt cgactgtcat
    19681 cccatcgaac atagattcac gatagaagtc gaggtgccca ctcgtttccc aaagctgggc
    19741 tttcccgata tggggcgttt gtactagatc gtagccgcgt tttaaatgtt ctttaatcca
    19801 gtaatccttg atattttgca aaagcaacgc accacgcgga taccaaagca ccagtccggg
    19861 gccaacctgt tcgttgattt cgaaaagttc gagactcttg ccgagtttgc gatgatcgcg
    19921 ccgcttggct tcctcgataa aattcaagta cttcttaagt tccttttgtg tggcaaaaga
    19981 agtaccataa atacgcgtta gcattttatt gcgcgaatca ccgcgccaat atgcgccagc
    20041 aacattcaat aatttaaagg ctttgatttt gccagtcgaa ggaatgtgag gaccacggca
    20101 aagatcggta aaatcgcctt gagtataaat tgacaatggc acatccggtt caatctcacg
    20161 aatgatctca actttataag tctcacccat cttggaaaat aattcaattg cttcctcgcg
    20221 ggaaacctca cgtcttataa ttggcaaatc tgccgcaatg atttcattca tacgggcttc
    20281 gattgccggc aaatcgccat ccgttaaagg tggttcgata tcaaaatcat agtagaaacg
    20341 attttcgatc gccgggccga tggttacttt tgcctgtgga tataattgct taaccgcctg
    20401 tgccataacg tgcgaggtgc tgtgcagtaa tatttcttgt gactcgggag aattataggt
    20461 cagaatctca agtcgagcat cttccgaaat aatagtcgaa agatcaacca agcggtcatt
    20521 gactttagcg gcgagagcgt tctgaaaaag accgggactg atttctttag cgatttgccc
    20581 ggctgataca cctgccgaca cttccatagt tgaattgtcg ggtaaatgta tcgtaatctt
    20641 tcgatttgat tccaaaattt gcttcctttt cgcgcttcag agtcataatg actatattta
    20701 atttttcaat tgtactaggg cgatactgga cttttctgac ccatcggggt tcctaataaa
    20761 gaaccagtgt tcccggtttt tatcaggata gatatattaa ccatctaacc tgaattcgat
    20821 tgtaaaaaaa ctcactttgg tgggcgatac tggacttgaa ccagtgaccc cctgcatgtc
    20881 aagcaggtac tctagccacc tgagctaatc gcccggatat atcaacttaa aataatggtc
    20941 caaaagttga taataattat tccaattcat tatcgtcaaa aaacgaatgc aaatttaacc
    21001 aaagcatatt tatcacgcaa aaagaaaaat tgacattgag gtccgcttaa tacataattc
    21061 tctgtttaat aaaaatacta aacgatttta ggtaatgaat tcgtttttta ttaattgatt
    21121 caaaaaactg ctctacatta atcctattta tttctaaaag gtggatacca tgtcttaata
    21181 ctttacataa aaaaccgatt caccccatgg ggtgaatgta aacgatgtca tgctatgtga
    21241 ctattagctc ctttcttcat ttttttctaa ggtattttca gataaaagca ttgttatgtt
    21301 ttgttatgat tcacttggaa atcgaaaatt attaagtttg aaataagagt caaaaagaaa
    21361 ggaaatcaag atgaatca
//
LOCUS       DAOOMU010000033        20777 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2524_length_20777_cov_18.199112, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000033 DAOOMU010000000
VERSION     DAOOMU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 20777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20777
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2524_length_20777_cov_18.199112"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..>1205)
                     /locus_tag="PLI55_04705"
     CDS             complement(212..>1205)
                     /locus_tag="PLI55_04705"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPB00167.1"
                     /translation="MKNEFPTLDFENNLWEQGISHIAGVDEAGRGPLAGPVVAAAVVF
                     DKSSEIISGIQDSKQISPRKRERLFDEIMSQAKAIGIGLVESEEIDRINILRATHKAM
                     RMAIGRLKINVQHILVDGKPLPDKIYPQTAIIGGDRKCYSIAAASIVAKVYRDRLMQQ
                     YDSVFPGYGFAEHKGYGTKKHLESIVKLKPCPIHRISFRGVTEYIVDINKSANTRWLG
                     KYGEDLAALFLFRHGYQIIQRNFHLGAYGEIDIIAQKDNNLCFVEVKTQRHTKFGPPE
                     GWVDERKMRQIGRIASGYLSQNPVENVDCRFDVVTVNLNGSEVQIEHLPDAFRL"
     assembly_gap    1206..1305
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1370..3346
                     /locus_tag="PLI55_04710"
     CDS             1370..3346
                     /locus_tag="PLI55_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HPB00168.1"
                     /translation="MKEFTIRSLIIGLIMAVVLGAANAYLGLKAGMTIAATYPAAVIG
                     MAILRMMKGTILEENFARTVGSIGESVAAGAIFTIPAFYIAGIWTDFATLPNYLIASS
                     IMLVGGVLGVLFVALLRRVMVEDVELPFPESVAAAEIHKAGQKGGTGAKFLFGAMGLG
                     AGIQALISLKVFTDLKDWFITFKKAIIQLGSSGSATGQSGMVLTAPRVSPAYIGVGYI
                     IGPKLASLNFSGGLLAWGLFVPILTYFIAPSILPDTTAVTTDTWMALSSQVWRNLVRP
                     IAVGGMLMSAIYTLFKMRKSLINGLSRSISDVKKAAQGVQVNERTEQDLKFTTVMFGI
                     SLAAVGTFFIYLFFAKNVIAALVATVVMIIAGFFFAAVSGYLVGIIGSSNNPISGLTL
                     STLIVAALLMVALGMKGTPGVAAVLGVAGVVCVSAAVAGEMLQDLKVGHILGGTPWKM
                     QIGDLIGVALSAVVMFIPLIILHQGDINMGGTGLGGTNLAAPQASLMAMLSQGIVSGE
                     MAWPLIIVGILLGLGFILMQVKSPMLVCVGMYLGLETTFAIFVGGLIRGLLDHYVTKR
                     RCNDAQKSRVENTGVLLAAGLIAGEALVGLLVALLAILKVGYDKWLPSIFNFLPFRFE
                     VSLLIFGLIGWILVSIPLKNAGKADEPAPPSAVM"
     gene            3365..3706
                     /locus_tag="PLI55_04715"
     CDS             3365..3706
                     /locus_tag="PLI55_04715"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPB00169.1"
                     /translation="MKNKKGEVLNLYEIVPRPLVNSECQADGRIVLLKPKFKNPFLIK
                     LLQRNRRDNFYKLKLDEIGSACWIKFDGYKSAGIICEELESQFGAQIQPVAERVGKFL
                     LMLKKANLIAY"
     gene            complement(4025..4228)
                     /gene="rpsU"
                     /locus_tag="PLI55_04720"
     CDS             complement(4025..4228)
                     /gene="rpsU"
                     /locus_tag="PLI55_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPB00170.1"
                     /translation="MVEVTVYDNEPLERALRRFKKKFEKAGILNDVKKRTYYEKPSVE
                     KRMRRAKAIKRLHRTNAQKTAIM"
     gene            complement(4252..5109)
                     /locus_tag="PLI55_04725"
     CDS             complement(4252..5109)
                     /locus_tag="PLI55_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPB00171.1"
                     /translation="MSKKDKRVGSDSNRALSKYLEEIGKFEPLSPDKEIELAQRLRAG
                     DRDALRELTESNLRFVVSVAKDYQGQGLPLTDLINEGNLGLIKAAERFDETRGFKFIS
                     YAVWWIRQSILQALAEHSRIVRLPLNRVGTISKITKEAEILEQQYERAPMQDEIASGL
                     AMESEEVSDAIRISRKHQSLNDPFRDGESNALIDVIEDDMQLPPDHPLMDESLKQEIR
                     AALNTLKKREREVIKMYFGIDREYALTLNEIGEEFNLTRERVRQIKENAIRRLSHKSR
                     SKNLKAYLG"
     gene            complement(5270..7276)
                     /gene="tkt"
                     /locus_tag="PLI55_04730"
     CDS             complement(5270..7276)
                     /gene="tkt"
                     /locus_tag="PLI55_04730"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPB00172.1"
                     /translation="MARLCANTLRLLAVDGVQKANSGHPGMPMGMADVATILWSKYLE
                     HNPGDPQWVNRDRFILSAGHGSMLLYSLLHLSGYDVTLEDLQQFRQWDSRTPGHPEYK
                     CLPGVETTTGPLGQGFATGVGMAIAAKITASRFNTDQFQIFGTHKIYGIVSDGDLMEG
                     LSHEAASFAGHLKLGNIIYFYDDNRITIEGKTDLTFSESVAKRFEAYGWEVLQADGHD
                     YISIAKALDEALSKPDRPKLIITRTHIGCGSPSKQDTSECHGAPLGEEEVKITKRNLG
                     FDENVSFYVPEEVRTVYAERRAELEKKYAQWQIEYQKWREANPELAKKYDESQNQVLS
                     IQQEEEIIKAIPSDALATRALSGKIMQKIAELLPNFYGGSADLAPSTSTYLKNLGDIA
                     PDNFSGRNFHFGIREHAMGAILNGLALYGGLIPFGATFLVFSDYMRPPIRLAAMMKLR
                     VIYVFTHDSIFVGEDGPTHQPIEQVASLRIIPNMTVIRPADGLEVAQAWISAINNLGG
                     PTALILTRQKVPVLNRLANFDRHLIDRGGYIISPEQNNALDLVLVASGSEVSVAIEAQ
                     KILQAEGRATRVVSMPSIEKFVRQTATYRESVIPKGKVPVVVIEAGRSTGWGDLIRNP
                     LLTITIDRFGASAPAKVLAEKFGFTAQQVVDRIRDWQVLTNIIV"
     gene            complement(7296..8390)
                     /locus_tag="PLI55_04735"
     CDS             complement(7296..8390)
                     /locus_tag="PLI55_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HPB00173.1"
                     /translation="MVNVTELDNFVRQVLIAHGLNAEDARITAEVLIRADRRGIGSHG
                     VARLKRYVDGLDSGVIRPNPDVKIIRETPVSLVIDGDGGMGQPIAYRAMQKCIEKART
                     AYMCFAAIRNSNHYGIAGYYVLMALAEGMMGVSLTNTTPLVVPTFGRNVTIGTNPIAI
                     GFPGKKERPFLLDMATSTVPRGKLEVYARQGESIPDCWACDENGKPSTDAAALLDDMI
                     NRRVGGLLPLGGGTELTGGHKGYGLSAVVDILCGVLSAGMVGTEIARYSNAPAGVSHF
                     IGAINPDAFVGTDLISEGIDKYIQMLRNADKAAGQERIYVAGEKEFLAEERYRDFVPL
                     QDKVFQTLNDIGTKVGLSITELKKERTKCE"
     gene            complement(8424..9287)
                     /locus_tag="PLI55_04740"
     CDS             complement(8424..9287)
                     /locus_tag="PLI55_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPB00174.1"
                     /translation="MVHKQKIKNIIFEYCGLAVGSAVMAGSLVFFLIPAKIAPGGVSG
                     LSIVLHYLFNLPVGLVMLVFNIPLFFLGLKILGKEFGPRTFFSFVLVSIFTDFYDKVL
                     GVQAATEDPLLASIFGGILLGVGLGVVFRFKGTTGGSDIVGQIIHKYSNVSVGTGILI
                     ADFFVITFAGITFRDVNLALYGFISLYFSSKVLDLILDGMDYARSFYIISEKQDEIIN
                     LITKEMDRGGTELLGKGFYTRKTRNVLYTVVTRREVATLRQEILKIDPKAFVIIANVH
                     EVIGEGFRKRI"
     gene            9456..10643
                     /locus_tag="PLI55_04745"
     CDS             9456..10643
                     /locus_tag="PLI55_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB00175.1"
                     /translation="MQNNQNPIDNKENKRTVRIFAWASFLNDMGSDIIYPVWPLFVTE
                     ILKANMAVLGFVDGLGEAVVSLAKALSGYWSDRIRKRKVFIWSGYLMGAASRIGYAIS
                     TVWQHLIPFRILDRAGKIRGAPRDAIIADVSTNDNRGRNFGLMRMADNLGAVVGILIC
                     LALFKKIGYRALFILAAIPSLISVLLILIKIKEPGHTEKVIYKGMTLRDLDRNFVLFM
                     ILSGIFAIGNFTYSFLLIRAKQIGFPEAFVPVLYLVYTLSASLTAYPFGRLADRIGRK
                     TVMQMSFLLWGLVCLDVIFFQSKIMIFLMFVIYGLHKGALEPVQSTIVSELAPEKFRA
                     SSLGTYQMLIGLCAFPASLIAGFLWDKIGSLAPFYLSLGLTIVASALMIFVKESKIIT
                     KSN"
     gene            complement(10644..11108)
                     /locus_tag="PLI55_04750"
     CDS             complement(10644..11108)
                     /locus_tag="PLI55_04750"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HPB00176.1"
                     /translation="MEVNDTNEVKPKPKNIALVAHDHKKKDLLEWVKFNEGTLKQHKL
                     HATGTTGKLLEKELKVKIHKLQSGPLGGDLELGAAIAQRKIDVLIFFWDPLEAQPHDP
                     DIKALLRISVVWNIPVACNRATADYIISSPLMNSVYQPTLPNYSEYLNRLKK"
     gene            complement(11147..12019)
                     /locus_tag="PLI55_04755"
     CDS             complement(11147..12019)
                     /locus_tag="PLI55_04755"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPB00177.1"
                     /translation="MKIGLLLSPKIEANDPIVPAIIELLIENKQTVIVADWQSNSLVT
                     NLNGVERCNPEQMPEKVDALFSIGGDGTFLSASHLMAKSRKPVLGIHLGGLGFLADVS
                     VDNYRERLIDFLNGKYSVEERQGLTARTYFKDSIQESFAFNDFVIDKGRISTMIKIRT
                     YINEEYLNTYRADGLIIATPTGSTAYSLSAGGPIISPELNVLVICPICPHSLSVRPVV
                     ISADQVVRIDCTDLSDDVSLISDGHNRIALGAAQKIEIHKAEFPLRIVRFSGDSFFHT
                     LRTKLNWGRDVRGN"
     gene            complement(12032..12295)
                     /gene="xseB"
                     /locus_tag="PLI55_04760"
     CDS             complement(12032..12295)
                     /gene="xseB"
                     /locus_tag="PLI55_04760"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPB00178.1"
                     /translation="MAKKSFSLEKSLERLEEILQILESGEKDIEESIKLFEEGMRLSS
                     ECQAHLQALEKRVKLLIEKSDGEICEQDFPESGETPETNNLFD"
     gene            complement(12316..13524)
                     /gene="xseA"
                     /locus_tag="PLI55_04765"
     CDS             complement(12316..13524)
                     /gene="xseA"
                     /locus_tag="PLI55_04765"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPB00179.1"
                     /translation="MAERIYTVTEIARDIKRVVEQFIAPVWVEGEVSNFTISRAGHIY
                     FSLKDQTAVLNCTIWRSQSLSIPFQIVNGMRLLVFGNITTYAMQSQYQLNVQQVRAAG
                     LGSLFLAFEALKKKLSAEGLFDAERKKPIPLYPTRIGLITSESGAALRDFLQISRRRN
                     PSIAIYIYPALVQGAEAAATIIAGIKLFNRLQNVDFIVITRGGGSLEDLWAFNEEPLV
                     RAVAQSAIPIVSAVGHEVDFTLCDFAADLRAPTPSAAAEMTIPERSHILNFIDQTCNK
                     MAQNIRMRLDNFHKEIDNITKRLFLNRPWELLRQRIQRIDELEGRIVKAINSRILSAQ
                     VELDNIHKTLEVLNPRAIMERGFAIVYHWPDKKIIKSISEVAVGEQVSVELKDGEFQA
                     KTEIIQSHKN"
     gene            complement(13712..>13931)
                     /locus_tag="PLI55_04770"
     CDS             complement(13712..>13931)
                     /locus_tag="PLI55_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPB00180.1"
                     /translation="INGFIETIKDALLNGERVDFRGFGNFSLKVRKPKKARNPGTNEE
                     ISLPARIVPVFKPSKLFKGEISRNYNQH"
     assembly_gap    13932..14031
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14148..15086)
                     /gene="nadE"
                     /locus_tag="PLI55_04775"
     CDS             complement(14148..15086)
                     /gene="nadE"
                     /locus_tag="PLI55_04775"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPB00181.1"
                     /translation="MESKLKYLQNSMAIEPEELASSLVEFIKVYVKKLEREGVIIGLS
                     GGIDSAVIAALCKRAVGAENTLALILPEKDSRKEHQVDAQIFANELGIATKLIDLTPY
                     LEMLEVYKLSPVNKPFLPEKIKGTLIGKAYDYYQRKTGETPFSASLTGLRDKEFDSYL
                     KKGNAYYRVKHRLRMLLLYLYAELENRLVVGSTNKSEYEIGFFVKYGCDVANDILPLL
                     NLYKTQVRELARYLNIPAKIREKAPSPDILPGLTDEKALSISYEKLDLILIAFAKSWE
                     VTEIAGALEISEKQVIEIERLIQKSEHMRKIYAPSI"
     gene            complement(15428..>16302)
                     /gene="lepB"
                     /locus_tag="PLI55_04780"
     CDS             complement(15428..>16302)
                     /gene="lepB"
                     /locus_tag="PLI55_04780"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPB00182.1"
                     /translation="IKIMKEQKQEKKTSGSKFWQEVKSWGIVLLIALGLKATIIEAYQ
                     IPTGSMENTILTGDFILGIKFIYGTRTPDWIGIPWTKTGFHIPWFRLPSFKKPEQGEV
                     VIFKYPVDPSLNYVKRCIAGPGQSVEIHDKVVYVDGKIFPNPKHAKFISQYVAPSGWN
                     DPEIFPDGIGNKDNCGPIYVPAKGDTLRFGQIQTGIIKNAAELKGHTFRIYQGKMYID
                     GNPAEYYIAEQDHYFMMGDNRDNSWDSRFWGFVPFDNVTGRGLITYMSWDKERPLYQL
                     DKKIRWWRIGRLVR"
     assembly_gap    16303..16402
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16608..18101
                     /locus_tag="PLI55_04785"
     CDS             16608..18101
                     /locus_tag="PLI55_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPB00183.1"
                     /translation="MVKNVVDILIQNRNNRADWPAVIFNEHTFTYSQIYDRSIYLAEF
                     LQKQKIQAGQTIGLLFQNKPEYLIAYYACLLNGFLPALLPTYLSTNKLTYNFNKLKIE
                     AVLYDSEFHKVVDEIELKKGSPLIKVPSSTWQDTSIDKTNIPTLSTDSFPGINYTNEP
                     AVVSFTDGNSGFPKAVVHSQAGLLSNAQNCVKLFGGMRAVRLLCTVPVFHFISHSFIP
                     HSISLAGGTVILVENSKTEEIIRAINSNKVNTLIGTPVFFKEFINYAKTDELSSLKHC
                     IVSGGCLSQETRQNLIKNLGVFVSELYGTTETQMISVVYDEIDQRECSIGQPFPEIDI
                     RIINSSGAIVTPGSVGELQVRSSALMLNYFDDLDKTHFNQTTWFSTGDIVRETSDGSL
                     IFEGRISDSIHKFGYDINPALIEKVLRQHPAIHEAVACPIKNPNHEDQIKVWVSLRES
                     ATVSIDDLNDFCRKNLPGYLQPDAIEVVTYFERDLSGKILRNLHNKS"
     gene            18129..20207
                     /locus_tag="PLI55_04790"
     CDS             18129..20207
                     /locus_tag="PLI55_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB00184.1"
                     /translation="MKEYQTEEIRNIVLLGHGSSGKTSLSEAMLYSAGATTRLGRVDE
                     GTTVSDYHDDEIERKFSISSSLLNCEWNKIKFNILDSPGYLDFVGEAKALTRVADLAL
                     ININGQSGVEVGTELVFKFAKEDDLPVWFIINMLDKEHADFQKSLNSIRSSFTKKAVQ
                     MQIPVNSGPNFDTIADVITQKVIKYKNDQKGTYTELEPTDEIKERLIALKNELIESVA
                     ESDDVLLEKYLSEGTISEEEFLNGLRNAIIKRNLFPVFVAAGLNNIGVTKIMDHIVNF
                     APTPSAKISVSGSYNGTPITRKISDSEPTSLFIFKTISEMHVGELSIFKVISGSLKSG
                     VDLLNSNNGKIERIGQIYCVNGKEKQEVAHLHAGDIGAVVKLRFSKTADTLCDQRAPI
                     TYPGISFPEPVIQIAIEPKSRGDEEKISAGLQSLTSEDPTMSWHYDPELKQTILSGQG
                     ELHLAIILQRLKQKFGVDVNQLPPRIPYRETIKKRGDSKYRHKKQTGGAGQFAEVWMY
                     VEPGPPGSGVEFTNTLVGQNVDRVFVPSVEKGVRSACEEGILAGYRVTDVKAVFYDGK
                     MHPVDSKDIAFQIAGKGAFKECFMNGNPVLLEPIYNLEVIVPEEYMGDVMGDISVRRG
                     KIQGVDSEGPFQKITAKVPLAELYRYSTALRSMTQGRGMHRQSFSHYEQMPKEIQDKV
                     VAASRKEHEE"
     gene            20213..20698
                     /locus_tag="PLI55_04795"
     CDS             20213..20698
                     /locus_tag="PLI55_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPB00185.1"
                     /translation="MEFDKLWAPWRIGYITNPKCEGCFLCRYPSESDDKKNLILQRGK
                     SAFVIMNFYPYNNGHLMVAPYQHTNDFAALDDATHLELFHLVEKSVGILRETLKAEGF
                     NIGVNLGTVAGAGLKDHLHIHIVPRWTGDTNFMPVIGHTKVISEGLTETWQKLKSKFD
                     E"
BASE COUNT         5780 a         4533 c         4107 g         6057 t
ORIGIN      
        1 agaaaaagaa acagaacatc cggaattttc gttcggctat aaattgctga aaataaatga
       61 gcacaaaaaa ttataactcc gattaatatt actgttaatg ttacttccat aatttcaccc
      121 cattctttcc attaataaca taacgatctt gatctatacc gtaaatatac gattccaaac
      181 atccccatta caaaaataat aaaggcgcta tttataggcg aaaagcatcc ggtaaatgtt
      241 caatttgaac ctcgctaccg tttagattga cagttacgac atcgaaccga caatcaacat
      301 tttcaactgg attctgggat aaatagccag aggcgatccg tccaatttgg cgcatttttc
      361 gctcatctac ccagccttca ggcgggccaa attttgtatg acgctgtgtt ttaacttcca
      421 caaaacagag attattatcc ttctgtgcga ttatatcaat ttcgccataa gcgccaagat
      481 ggaaattacg ttgaattatt tgatagccgt gccgaaatag aaaaagcgcc gctaaatcct
      541 caccatattt cccgagccag cgggtattcg ccgacttgtt tatatccaca atatattctg
      601 taaccccgcg aaaggatata cgatgtattg gacaaggttt caacttcacg atagattcaa
      661 gatgtttctt agttccgtaa cctttatgtt cggcaaagcc ataacccgga aagacggaat
      721 cgtactgttg catcaggcga tcgcggtaaa ctttggcaac tatcgaagcc gccgcgattg
      781 aataacactt acgatcgcca ccgattatcg ccgtttgtgg gtatatttta tccggtagag
      841 gtttgccatc aaccaaaata tgctgaacgt tgatctttaa gcggccaatc gccatacgca
      901 tagctttgtg cgtcgctctc aggatattta tacgatcaat ctcttccgat tcaactaaac
      961 cgatgccaat cgcctttgct tgactcatta tttcatcgaa caatctttct cgttttcgtg
     1021 gagaaatttg cttggaatct tggattccac taataatttc actgctttta tcaaaaacta
     1081 ctgcggcggc aactaccggt ccagctaatg gtccgcgccc ggcttcatcc acaccggcta
     1141 tatggcttat tccctgttcc cagagattat tttcaaaatc cagagttgga aattcatttt
     1201 tcattnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttaaa cacaggaggc
     1321 acaattgact gagcataagt ttaagccgtt cgtaccggct aatacggata tgaaagaatt
     1381 cactatccgc tcattgatta tcggtttgat tatggcagtc gttttgggcg cggcgaacgc
     1441 ctatctcggc ttaaaagccg gaatgacgat tgccgctact tatcccgccg ccgttattgg
     1501 tatggcgatt ttgcgtatga tgaaaggcac gattctcgag gaaaattttg cccggacggt
     1561 cggctcgatt ggtgaatcgg ttgccgctgg tgcgattttc acgattccgg cattttatat
     1621 cgctggaatt tggacggatt ttgccacttt accgaattat ctgatcgcca gttccattat
     1681 gctggtcggc ggtgttctcg gtgtactgtt cgttgcactc cttcggcggg taatggttga
     1741 agatgtggaa ctgccatttc cggaatcggt tgccgcggct gaaattcaca aagccggtca
     1801 gaaaggtggt actggagcta agtttctgtt tggcgcaatg ggtttaggcg ctgggattca
     1861 ggctttgatt tcattaaaag tcttcactga tctcaaggat tggttcatca ccttcaaaaa
     1921 agccatcatt caacttggta gcagtggttc ggcaaccggt cagagcggga tggttctgac
     1981 cgcgccgcgc gttagtccgg cttatatcgg agtcggctac attattggtc cgaaattagc
     2041 ttcgctcaat ttcagtggcg gtttgctggc gtggggattg tttgtaccga ttttgactta
     2101 ctttattgcg ccttccatac tgccggatac gactgcagtt accactgaca cctggatggc
     2161 actttcatct caggtctggc gtaatttggt gcgaccaata gccgtcggtg gtatgttaat
     2221 gagcgcaatc tacacccttt ttaaaatgcg taaaagccta atcaatggac tttcccgcag
     2281 tatcagcgat gttaaaaaag ccgctcaggg ggttcaagtc aacgaacgca ccgagcagga
     2341 tttaaaattc acgactgtta tgttcggtat ttctctggcg gcggtgggaa ctttctttat
     2401 ttatttattt tttgcgaaaa atgtcatagc cgcattagtg gcaacggtcg taatgatcat
     2461 cgccggtttc ttttttgcgg ctgtttccgg ttatctggtt ggtattattg gttccagtaa
     2521 taatccaatt agtggactga cactttcaac tctcatcgta gccgctcttt tgatggttgc
     2581 tctcggtatg aaaggcacgc ccggagtagc ggccgtgctt ggcgttgcag gtgtagtctg
     2641 tgtttctgct gcagtagctg gtgaaatgtt acaagactta aaagttggtc acattctcgg
     2701 cggaacaccc tggaaaatgc aaatcggcga tttaattggt gtagctcttt ctgccgtagt
     2761 tatgttcatt ccgcttataa ttctccatca gggcgatatc aatatgggcg ggactggtct
     2821 gggcggtact aatcttgccg caccgcaagc cagtttaatg gcaatgttat cacaaggtat
     2881 cgtcagcggt gagatggcat ggccattgat aatcgtcgga attttgttgg gactgggatt
     2941 cattttaatg caggtgaaaa gcccgatgtt ggtctgtgtc ggtatgtatt tgggattgga
     3001 aacgaccttc gccattttcg tcggcggttt aataaggggt ttacttgacc attatgttac
     3061 caaacgtcgc tgtaatgatg cccaaaaatc acgcgtggaa aatacgggag tcttgttagc
     3121 cgctggattg atcgctggtg aagctttagt cggtttatta gtagcattac tcgctattct
     3181 gaaagtcgga tacgacaaat ggttaccgag catttttaat tttcttccgt tccgattcga
     3241 agtcagtttg ttaattttcg gtttaattgg ctggattttg gtcagcattc cattgaaaaa
     3301 tgcgggcaaa gccgacgaac cggcgccacc atcagccgtt atgtaatcgc aaggaagttt
     3361 tttgatgaaa aataaaaagg gcgaggtatt gaacttgtac gagatcgtgc ctcgcccttt
     3421 ggttaatagc gaatgccagg ctgatggcag aatcgtatta ctcaaaccta aattcaaaaa
     3481 tccatttttg attaagctgc tccagcgtaa ccgacgcgat aatttttata aattgaaact
     3541 ggatgaaatt ggttcggctt gttggataaa attcgacggc tataaaagcg ccgggattat
     3601 ctgtgaagaa cttgaaagtc agttcggtgc gcaaatccaa ccagttgcag aacgagttgg
     3661 aaaatttctc ctaatgctaa aaaaagccaa tctaatcgca tattgatttc tcaatatatt
     3721 ttacaattta gcgagaagcg aaaagcatat acctgtgtat ctcatttaac ttcgattgaa
     3781 ctgcctacga taaatttcct cggtcagtaa tagcttagga aacctaaaat atagatatat
     3841 taccgacttg ggaagtagtc tgtctaatta tttataaaat attctcaagc aagaaataac
     3901 caacaaaatt cggattaatc ttgctgacct aaatcactcg actaccgcca aattccaacg
     3961 aaaaaatgtt aaaacttttg aggtaataac caaaaaatat attacgtaat aacgcccggt
     4021 aattttacat tattgccgtt ttttgggcat tggtacggtg tagacgtttg atggcttttg
     4081 cccgacgcat tcttttttcg acactcggtt tttcgtagta tgtccgtttc ttgacatcat
     4141 tcaaaatacc agctttttcg aactttttct taaagcgtct taaagctctt tccagtggtt
     4201 cattgtcgta cacggtaact tcaaccatgc aatcagtatc ctttctactg tttagccgag
     4261 gtaagctttt aagttcttac ttcgtgattt atgacttaaa cggcgaatag cattttcctt
     4321 gatctgacgg actctctccc gcgtcagatt gaattcttcg ccaatttcat taagagttaa
     4381 agcatattcg cgatctattc cgaaatacat ctttatcact tcgcgttcac gctttttcag
     4441 cgtgttcaaa gccgcgcgga tttcctgttt cagcgactca tccatcaatg gatgatcggg
     4501 tggtaattgc atatcgtctt cgataacgtc aatgagagcg ttggactcgc catcccggaa
     4561 cggatcattt agcgactgat gtttacgtga aatgcgaatg gcgtcggaaa cttcctcgga
     4621 ttccattgct aaaccggagg caatttcatc ctgcatcggt gctcgttcat actgctgttc
     4681 cagaatttca gcctctttgg tgattttgct gattgtgccg acgcgattaa gcggcaggcg
     4741 tacgatccgc gagtgttcag ctaaagcttg cagaattgac tgtcgaatcc accatacggc
     4801 gtaagaaatg aacttaaaac cgcgggtttc atcgaaacgc tcggcggctt taatcagccc
     4861 taaattaccc tcattgatta aatcggtcaa aggcaaaccc tgtccctgat aatccttagc
     4921 cacactgaca acgaaccgta aattcgattc agttaattcg cggagtgcat cccgatcacc
     4981 ggcgcgcaaa cgttgggcaa gttcaatttc cttatcgggc gacaaaggtt caaatttccc
     5041 aatttcttcc aaatatttgc tcaatgcgcg gttggaatca ctgccaaccc ttttgtcttt
     5101 tttcgacata aactacgaac cttcgctttc actataaatt aaaagcctgc aaaaatacta
     5161 aaaattttag attctaccaa tatggagaag ataatttttg atgcataaat aattattatt
     5221 ttaatgcaaa atctgccaaa atccaaatat tgagatcttc aaatccctac tatacaataa
     5281 tatttgtcag tacttgccag tctctaatcc gatcaacaac ctgctgggcg gtaaaaccga
     5341 atttttcggc taacactttg gcgggcgcgg aagctccaaa gcgatctatg gtaatggtca
     5401 gcaatggatt acggattagg tcaccccaac cggtgcttcg tccagcttcg ataactacta
     5461 ctggcacttt acctttcgga atgacagatt cacgataggt tgcggtctgc cgtacaaatt
     5521 tttcgataga tggcatcgaa acgacccgtg tcgcacgtcc ttcagcctga agaattttct
     5581 gcgcttcgat agcaaccgac acctccgaac cgctggctac caagactaag tctaaagcat
     5641 tattctgttc tggagaaatt atatagccgc cacgatcaat caaatgacga tcaaaattgg
     5701 ctaggcgatt caacactggt actttttggc gagtcaaaat caaggcagtc ggtcccccta
     5761 gattattgat agcagatatc caggcttgag caacttccag cccatccgcc ggccgaatta
     5821 ccgtcatatt cggaataatg cgcaaagatg ctacctgctc gatcggctga tgagttggtc
     5881 cgtcttctcc cacaaaaata ctgtcgtggg taaaaacata aataactcgc aacttcatca
     5941 ttgccgccaa ccgaatagga ggacgcatat aatcggaaaa aaccaaaaaa gtcgctccaa
     6001 aaggaatcaa accgccatac agtgccaagc catttagaat tgcacccatc gcgtgttcac
     6061 ggataccaaa gtgaaagttg cggccggaaa aattatccgg agcaatatcg cctaaatttt
     6121 tcaaataggt actggtcgaa ggtgctaggt cagccgagcc accataaaaa ttcggcaaaa
     6181 gttcggcaat cttctgcata attttaccgg agagagcccg cgttgccagg gcatctgacg
     6241 gaatggcttt gataatttct tcttcttgtt gaatactgag gacttgattc tgcgattcat
     6301 catatttttt tgccagttcc ggattagcct cgcgccattt ttgatattcg atctgccact
     6361 gagcatattt tttctcaagt tcagcgcgcc gttcagcgta aaccgtgcgt acttcctccg
     6421 gaacgtagaa agagacattt tcatcaaagc cgagattgcg tttggtgatt ttgacttctt
     6481 cttctcccaa tggagcacca tgacattcgg aagtatcttg tttactggga ctgccacaac
     6541 caatgtgggt tcgagtaata ataagttttg ggcgatcagg cttggataaa gcttcatcta
     6601 aagccttagc gatactgatg taatcgtgac cgtcagcttg gagaacttcc cagccatagg
     6661 cttcaaatct tttggcaacc gattctgaaa aagtcaagtc ggttttgcct tcaatagtta
     6721 tccggttatc atcataaaag tatataatat tgccaagttt gagatgaccg gcgaatgaag
     6781 cggcttcgtg cgaaagcccc tccatcaaat caccgtcact tacaattcca tagattttat
     6841 gcgtcccaaa aatttgaaat tgatcagtat taaagcggct agcggtaatt ttggcggcga
     6901 ttgccattcc gacaccggtg gcaaaaccct gaccaagcgg accggtagtc gtttcgacac
     6961 ccggtaaaca tttatactcg ggatgacccg gcgtgcgcga atcccattgt cgaaattgct
     7021 gcaaatcttc cagagtgaca tcatacccac tcagatgtaa caatgagtac aatagcatcg
     7081 aaccgtgtcc ggctgaaagg ataaaccgat cgcggttaac ccattgcgga tcaccgggat
     7141 tgtgttcaag atacttgctc cacaaaatag tagcgacatc tgccattccc atcggcattc
     7201 cgggatgccc cgagttagct ttttggacac catcaaccgc caatagacgc agcgtattgg
     7261 cacacagacg agccatttcc agattaatgt tttgcttact cacactttgt cctttccttt
     7321 tttagttcag taattgataa acctacttta gttccaatat catttaaagt ctgaaaaact
     7381 ttatcttgta atggtacaaa atcgcgatac ctctcttcgg cgagaaattc cttttcaccg
     7441 gctacataaa tccgttcctg accggcggct ttgtcggcat ttcttaacat ttggatatat
     7501 ttatcaattc cttcgctaat taaatcagtt cccacaaatg cgtccggatt tatcgcgccg
     7561 ataaagtgag ataccccagc cggtgcattg gaatagcggg caatttcggt tccaaccatt
     7621 cccgcagata aaactccaca caaaatatcc actaccgcgc tcagaccata acctttatgt
     7681 ccgccggtca gttcagtacc gccacccagt ggcaataaac caccaacccg acggttaatc
     7741 atatcatcaa gtaaagccgc cgcgtcggta gaaggtttac cgttctcatc acaagcccaa
     7801 caatccggaa tcgattcacc ttggcgcgca taaacttcca atttgccgcg cggcacagtc
     7861 gaagtcgcca tatccagcaa aaacgggcgt tcctttttac cgggaaatcc gattgcaatc
     7921 ggattagtgc cgatcgtaac gtttcgtcca aaagtcggca caaccaacgg cgtggtattt
     7981 gttaacgaaa ctcccatcat tccttccgcc agtgccatta aaacgtaata accggcaatc
     8041 ccataatgat tcgaattccg aattgccgca aaacacatat aggcagtgcg cgctttctcg
     8101 atacatttct gcatcgcgcg ataagcaatt ggttgcccca taccgccatc gccatcaata
     8161 accagtgaaa ccggcgtctc gcgaattatt ttgacatccg gattaggtcg aatgaccccg
     8221 ctatctaaac cgtcaacata tcttttcaaa cgcgccacac cgtgtgagcc gattccgcgg
     8281 cgatcggcgc gaattaatac ctccgccgta atacgggcat cttcggcatt caagccatgg
     8341 gcaattaaaa cctgccggac aaaattatcc agttcggtta catttacaat gacttctttt
     8401 gaaataacgc ctcctatatc aaattaaatt cgtttgcgaa aaccctcacc aatgacttca
     8461 tgcacatttg cgataataac gaaagctttc gggtcaatct taagaatttc ctgtcgcaaa
     8521 gttgcaacct cccgacgagt caccaccgta taaagaacat tgcgagtttt acgggtataa
     8581 aaccccttcc cgagaagttc ggttccaccc cgatccatct ctttagtaat caaattgatg
     8641 atttcatcct gtttttcact aataatataa aaagagcggg cataatccat accatccagg
     8701 attaaatcca gcactttgct ggaaaaatat aaactgatga aaccatacaa agccaggttg
     8761 acgtctcgga aagttatccc ggcaaaagta ataacgaaaa aatccgcgat caatatccca
     8821 gttccgaccg aaacattact gtatttatgg ataatttgcc cgacgatgtc cgagccaccg
     8881 gttgtccctt taaagcgaaa aacgactccc agtccaactc ccaagagaat accgccaaaa
     8941 attgaagcca ataacggatc ctcagttgcc gcctgtactc ccaaaacttt atcataaaaa
     9001 tcggtaaaaa tcgaaaccaa cacaaatgag aaaaaagttc gtggcccaaa ctccttgccc
     9061 agtattttca atcctaaaaa aaacagaggg atattgaaaa ccaacatcac cagaccaacc
     9121 ggtaaattga acaagtaatg taaaacaatt gataaaccgc tcactccgcc gggagcaatt
     9181 ttggccggaa tcaagaaaaa aaccagactg ccagccataa cagccgaacc aaccgccaac
     9241 ccacaatatt cgaaaatgat gttttttatt ttttgtttat ggaccatata aaactatatt
     9301 atattttcaa ttttactact tattacggaa aaaatacgac catagtttaa taaattttta
     9361 acaaactttt ataataagct agctaaatta tttacagtta ttgcctattc aattatttca
     9421 gaataatttc atagggttac aattggagaa aaacagtgca gaataaccag aatcctattg
     9481 ataataaaga aaataaaaga actgtcagaa tttttgcctg ggcttcattc ctgaatgaca
     9541 tgggctcgga tataatttat ccggtttggc cattattcgt tactgagatt ctgaaggcta
     9601 atatggcggt gttgggattt gttgatggat tgggagaagc ggttgtttct ttagccaaag
     9661 cattgtccgg ctattggtca gatcgtatcc gtaaacgaaa agtttttatc tggagtggtt
     9721 atttgatggg agccgcatcg agaatcggct atgccatttc cacagtctgg caacatttga
     9781 tcccgtttcg aattctggac cgcgctggaa aaatcagagg tgcgccgcgc gatgctatta
     9841 tcgccgatgt ttcaaccaat gacaatcgcg gtagaaattt cggattgatg cggatggctg
     9901 ataatttagg tgccgtggtc ggtattttga tttgtttagc attattcaaa aaaatcggat
     9961 atcgcgccct tttcatactg gcggcaattc cgtcgcttat ttccgtgttg ttaattttaa
    10021 ttaaaatcaa agaacccggt catactgaaa aagtcattta taaaggaatg actttacgcg
    10081 atttagaccg taatttcgtt ttatttatga tcttaagcgg aatttttgcc atcggtaatt
    10141 tcacttattc atttttacta atacgcgcca aacagatcgg ttttccagaa gcgtttgtgc
    10201 cggtgttata cttggtttat accctttcgg catcactaac agcttatcct ttcggtcggt
    10261 tggcggaccg gattggtcgt aaaaccgtta tgcaaatgtc atttctgctc tggggattgg
    10321 tctgtcttga cgttattttc ttccaatcca aaattatgat atttttaatg tttgttattt
    10381 acggtttgca taagggcgca ctcgagccgg ttcagagtac aattgtttct gaattagctc
    10441 cggaaaagtt ccgcgccagc agtctgggca cttatcaaat gttaattgga ttatgcgctt
    10501 ttccggcgtc tttgattgcc ggatttctct gggataaaat tggttcgctg gcaccgttct
    10561 atttatcact gggattgaca atcgtagcca gcgctttgat gatctttgtc aaagagtcga
    10621 aaattattac taaatccaat tgattatttt ttcaaacgat ttaaatattc ggaataattg
    10681 ggtaaggtcg gctggtaaac cgaattcatt agcggcgacg aaatgatgta atccgcggtc
    10741 gcccgattgc aggctaccgg aatattccag acaaccgaaa ttcgcagaag cgctttaata
    10801 tccgggtcat ggggttgagc ttccagcgga tcccaaaaaa agatcagtac atctattttg
    10861 cgttgagcga tcgccgcgcc cagttccaga tcgccgccga gcggaccgct ctggagttta
    10921 tggattttga ctttcaattc cttttcgagt agttttccag tggttccagt cgcatgcaat
    10981 ttatgttgct tcagcgtacc ttcgttaaat tttacccatt ctaacaagtc tttcttttta
    11041 tggtcatgcg caactagcgc tatattcttt ggcttaggtt ttacttcatt ggtatcattc
    11101 acttccattg aatttctcct tatactttat cttttttaat tataaactaa ttccctctga
    11161 cgtctcttcc ccaattcagt ttggtgcgca aagtatgaaa aaaggaatcg ccgctaaagc
    11221 gcacaattcg aagcggaaat tcagctttat gaatctcgat tttttgagcc gcgccgaggg
    11281 caattctatt atggccatca ctaattagcg aaacgtcgtc gcttaaatcg gtacaatcaa
    11341 ttcgcaccac ctgatcggcc gagataacca ccggccggac tgatagacta tgcggacaaa
    11401 tcggacaaat taccagcaca tttaattccg gtgaaataat cggtccaccg gcagacagag
    11461 aatatgccgt agaaccggtg ggagtcgcga ttatcaatcc atcggcgcga taggtgttca
    11521 aatattcttc gttgatatag gtgcgaatct taatcatcgt ggagatccgc cctttgtcaa
    11581 tcacaaagtc gttaaaggca aaactctcct gtatggaatc tttgaagtaa gtgcgagccg
    11641 tcaatccttg ccgctcttca accgaatatt taccattaag aaaatctatc aaacgctcgc
    11701 gataattatc aacagataca tcagctaaaa atcccaatcc tcccagatga attccaagca
    11761 ctggtttacg actttttgcc attaaatgcg aagcacttaa gaaagtaccg tcgcctccaa
    11821 tgctgaaaag tgcatcaact ttctccggca tttgctccgg attacatctt tcgacaccat
    11881 tcaaattggt aacaagacta ttactttgcc aatcagccac gattacagtt tgcttatttt
    11941 caatcagtaa ttcgataatc gccgggacga ttgggtcgtt tgcttcaatt ttcggtgata
    12001 aaagtaaacc gattttcatt ctatatcatc cctaatcgaa taagttgtta gtttctggag
    12061 tttcgcccga ttccggaaaa tcctgttcgc agatttcgcc gtcgctcttt tcgatcaaca
    12121 gtttcactcg cttttccagt gcctgtaaat gcgcctggca ttcgctcgat aggcgcattc
    12181 cttcttcgaa aagtttgatt gattcttcga tatctttttc acctgattct aaaatctgta
    12241 aaatttcttc aagccgctcg agactttttt ccaatgaaaa actttttttc gccatttatt
    12301 tacctcgtta attaactaat ttttatgtga ttgaattatc tccgttttag cctggaattc
    12361 accatctttc agttccacgc ttacttgttc gccgactgct acttcactga ttgatttaat
    12421 gattttttta tccggccaat gatacacaat ggcaaaacca cgttccatta tggctcgtgg
    12481 attcaagact tccagtgttt tatgaatgtt atccaattca acttgggctg ataggatccg
    12541 agagttaatc gctttgacaa ttcgcccttc taattcgtca atccgttgaa tacgttgacg
    12601 taacaattcc caggggcggt ttaaaaaaag gcgtttggtt atgttgtcaa tctccttatg
    12661 aaaattatca agccgcattc tgatattttg agccatttta ttacaggtct gatctataaa
    12721 gtttaggata tgacttcttt caggaattgt catttcagcc gcggctgaag gagtcggcgc
    12781 ccgcaaatcc gccgcaaaat cgcagagggt gaaatcaact tcatgtccga ccgcactgac
    12841 gatcggaata gccgattgag ccacggcgcg gaccaacggt tcttcattga aagcccagag
    12901 gtcttctaat gaaccgccac cgcgggtgat tacgataaaa tctacatttt gtaaacgatt
    12961 gaaaagtttt ataccggcaa ttatagttgc ggctgcttcc gctccctgca ccaaagccgg
    13021 atagatatag attgcgatcg agggattccg tcgcctgctt atctgcagaa aatcacgtaa
    13081 agccgcaccg ctttcactgg taattaaacc gattcgggtg ggataaaggg gaataggctt
    13141 tttgcgttcc gcatcgaaca agccttccgc gctcaacttt ttcttcaaag cctcaaaagc
    13201 cagaaataaa ctgcccaaac cggccgcgcg cacctgctga acattcaact gatactgcga
    13261 ttgcatcgcg taggtcgtaa tatttccaaa aacaagcagg cgcattccgt tgacaatttg
    13321 aaaaggaata gacaatgatt gactacgcca gatggtgcag ttcagcactg cggtttgatc
    13381 ttttaaagaa aaatagatgt gaccggctcg actgatggtg aaattcgata cttcaccttc
    13441 aacccagacc ggcgcgataa attgctctac aacccgcttg atatctcggg caatttcagt
    13501 gacggtataa attcgctctg ccataaaaaa aagagtggtg cggacacgaa ggccatcacc
    13561 actctccttt tgtacaaatt aactacctaa tcttcttctt atgacggtct cttctaagcc
    13621 gtttcttacg cttatgggta gcaatttttc ttttttttct ttttcttcca cagggcataa
    13681 cccatttact cctattattt cactacaaac tctaatgctg gttatagttg cgactgatct
    13741 cacctttaaa cagtttagac ggtttaaaaa caggaactat acgcgcgggc aaactgattt
    13801 cttcattcgt ccctggatta cgggcttttt taggtttgcg aacttttaaa ctaaaattgc
    13861 caaaacctct gaaatccacg cgctcaccgt ttagcaaagc atccttgatg gtctcgataa
    13921 aaccattaat cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttatcaaat
    14041 attctcaatg ttaaaaaaac ttatatttta gattgtaaaa atacgtcagg cattgatgat
    14101 taacaagcct tttttcaaaa ttgaataaat gggtttccct gctaacatta tatagaaggg
    14161 gcgtagatct tccgcatatg ttccgatttc tgaattagac gttcgatttc tataacttgc
    14221 ttttctgaaa tctcaagtgc accggcaatt tctgtaacct cccaactctt tgcgaaagcg
    14281 atcaaaatta aatccaactt ctcataggag atggagagcg ctttttcgtc ggtaagtccc
    14341 ggaagaatat cgggcgaagg ggctttttcc ctaatctttg ccggaatatt caggtatcga
    14401 gctaattctc ttacctgagt cttatacaag ttcaacagcg gcaaaatatc attcgccaca
    14461 tcacagccat atttcacaaa aaagccgatt tcgtattcac ttttattagt cgagccaact
    14521 accaacctat tttcaagctc cgcatacaaa taaagtaaaa gcattcgcag tctgtgttta
    14581 acccgatagt aggcattgcc cttttttaag tatgaatcaa attccttatc ccttaacccc
    14641 gtgagactgg cggagaaggg agtttccccg gtttttctct ggtaataatc gtacgctttt
    14701 ccaattaaag ttccctttat tttttccggt aagaacggct tgtttacagg cgataattta
    14761 tatacttcaa gcatctcaag atacggcgtt aggtcaatta atttcgtcgc aatgcctaat
    14821 tcattggcaa aaatttgcgc atctacttga tgctcctttc tggaatcttt ttcgggtagg
    14881 attaaagcca acgtattttc cgcaccgacg gctcttttac ataaagcggc aatcacggct
    14941 gaatcaatgc caccgctcaa tccaattatt actccttccc tttccagctt tttcacatac
    15001 accttgataa attctactaa agaactggct aattcctccg gttcgattgc cattgaattt
    15061 tgtaaatatt ttaatttcga ttccacgttt tctctcaaat attcagcagg ctcagcctta
    15121 cttatcaaaa cttaacacag tttagcaata ctttggataa atttgctggg attttctctt
    15181 ttgcttctaa agagggaaaa tactgcggca acgccagctc ggactcctcc atctagtaaa
    15241 gcaggtaagt aattttttaa tcttttccag cactaataaa cattaaacaa tagaaaaatt
    15301 tattaacaat ccaaatagga taaattccag ttaaaccggt taaataaccg gaaatgaaat
    15361 ttccgatcca aacgtcagta acttctttta attaaagatg ataatattga aataaattta
    15421 gaagttttta gcggacgagg cgtccgattc tccaccagcg aattttctta tccagttgat
    15481 agagcggacg ttctttatcc caggacatat acgtaatcaa tccccgtccc gtaacgttat
    15541 cgaatggcac aaagccccag aaccggctgt cccagctatt atcgcggtta tcacccatca
    15601 taaagtagtg atcctgttcg gcaatgtaat actcggctgg attaccgtca atatacattt
    15661 tcccctgata aatgcggaag gtatgacctt ttagctcagc cgcatttttg atgataccgg
    15721 tttgaatttg gccaaaacga agcgtgtcgc ctttggcagg aacataaatc ggtccgcaat
    15781 tatctttatt gccgatgccg tcgggaaata tctcaggatc attccaacca ctgggcgcta
    15841 catactgcga aataaacttc gcatgtttag gattcgggaa aattttacca tccacatata
    15901 ccactttgtc atggatttcg accgattggc caggcccggc gatacagcgc ttgacataat
    15961 ttagcgacgg atcaactgga tatttgaaaa taacgacctc gccctgttcc ggctttttaa
    16021 atgacggcaa acgaaaccaa gggatatgaa aaccagtttt tgtccaggga attccgatcc
    16081 aatccggtgt ccgagtgcca taaatgaatt ttataccgag aataaaatca ccggtcaaga
    16141 tggtattttc catcgaaccg gtcggtattt gataggcttc gatgatggta gcttttaaac
    16201 ctaaagctat gagcaggaca attccccagg acttgacctc ctgccagaat ttgctcccac
    16261 ttgtcttctt ttcttgcttt tgttctttca taattttaat agnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16381 nnnnnnnnnn nnnnnnnnnn nnccgatttg accttaaatc attttattcc tctcaaattt
    16441 gatagcggat tagcaaataa taaaatcctc tctaatatct tttaatttat taaattagat
    16501 acgctttcca attgaacaat aacttccgga ttattcaatt tacttggaat tatttggaga
    16561 atttgattaa aattgcgcgc tttttatttt aattggcttt tatcgtgatg gttaaaaacg
    16621 tcgtcgacat actaatccaa aatcgaaaca atcgggcgga ctggccggct gtaattttta
    16681 atgaacacac cttcacctat tctcaaattt acgaccgatc tatttaccta gccgagtttt
    16741 tacaaaaaca gaaaatacaa gcagggcaaa caatcgggtt gttatttcaa aacaagccgg
    16801 agtatctgat cgcttattat gcttgccttt tgaacggttt tttaccagca cttttgccga
    16861 cttacctctc cacaaacaaa ctcacctata actttaataa actaaaaatt gaagcggttc
    16921 tttatgactc ggaatttcat aaggttgtgg atgaaatcga actgaaaaaa ggttcgcctc
    16981 tcataaaagt accatccagc acctggcaag atacttcgat tgataagact aatataccga
    17041 cgctatcaac cgattcgttt ccaggaatta attacacaaa tgaaccagct gtggtatcgt
    17101 tcaccgacgg caattccggt ttccctaaag ccgttgtaca tagtcaagcc ggtttacttt
    17161 ccaatgccca aaattgcgtg aaattgttcg gtggtatgcg cgctgtgcgt ttactttgta
    17221 ccgtaccggt ttttcatttt atttcccaca gttttatccc gcattctatt agtttagcgg
    17281 gcggaactgt aattttagtt gaaaactcaa aaacggaaga aatcattcgg gcaataaatt
    17341 caaataaagt caacactttg attggaactc cggttttttt caaagaattc attaactatg
    17401 ccaaaactga cgaacttagt tctttgaaac attgtatcgt ttctggtggc tgtttaagtc
    17461 aggaaactcg ccagaatttg ataaaaaatc tcggcgtttt cgtctcggaa ctttatggaa
    17521 caaccgaaac ccagatgatc tccgtcgtct atgatgaaat tgatcaacgt gaatgtagca
    17581 tcggtcagcc gtttccggag attgacattc gtatcatcaa ttcatccggt gcgattgtta
    17641 ctccgggttc agtcggtgaa ttgcaagttc gctcgtcagc cttgatgttg aattattttg
    17701 atgacttgga taaaacccat tttaatcaaa ctacctggtt tagtactggg gatattgtga
    17761 gagaaaccag cgacggctcg ttgattttcg aaggaagaat ttctgattct atccataaat
    17821 tcggttatga catcaatcct gctttgatcg aaaaagtttt acgacagcat ccggcaattc
    17881 acgaagcagt ggcttgccct attaaaaacc ctaatcacga agatcagatt aaagtgtggg
    17941 tatctttgcg ggaatcagca accgttagca ttgatgactt gaatgatttc tgtagaaaaa
    18001 atttaccggg gtatcttcaa ccagatgcaa tcgaagtcgt aacatatttt gaacgagacc
    18061 tatccggaaa aattcttcgt aatctgcata ataaaagtta aattttcgcg ataatatgga
    18121 ggctaatttt gaaagaatat caaactgaag aaataagaaa tatcgtgctt ttgggacatg
    18181 gttcgtctgg aaaaaccagt ctgagtgagg caatgcttta ctctgccggc gccacgacca
    18241 gattaggtcg ggttgatgaa ggaacaacag tttccgatta tcatgatgat gaaatcgaac
    18301 gtaagttttc gatttcctct tcattactga attgcgagtg gaataaaata aaattcaaca
    18361 tcttagattc gcctggttac ttagatttcg ttggtgaagc caaagctcta acgcgagtag
    18421 ctgacctggc acttatcaat atcaatggtc agtcgggagt cgaagttggc actgaattag
    18481 tttttaaatt tgccaaagag gatgatttac cggtttggtt tattattaat atgctcgaca
    18541 aagaacacgc ggatttccag aaatcgctga attcgattcg ttcttccttt accaaaaaag
    18601 ccgtgcaaat gcagattccg gtcaatagtg gtccgaattt cgatactatt gccgacgtta
    18661 taacccaaaa agtcatcaaa tataaaaatg accaaaaagg tacttacacc gagttagaac
    18721 ccaccgatga aatcaaggaa agattaatcg ctctgaaaaa tgaattaatc gaatcagtgg
    18781 ctgaatctga tgatgtttta cttgagaaat atttaagcga aggtactata tccgaagagg
    18841 aatttttaaa cggcttacgc aatgcgatta tcaagcgcaa tctgtttccg gtttttgtag
    18901 ccgccggcct caacaatatc ggtgtcacga aaattatgga tcatatcgtc aattttgctc
    18961 caacaccttc cgctaaaatt tcagtcagcg gcagttataa tggcactccg ataacccgta
    19021 aaattagcga tagtgaaccg acttctcttt tcatttttaa aactatcagc gaaatgcacg
    19081 ttggagaatt atccatattt aaggttattt caggttcgct aaaaagtgga gttgatctgc
    19141 tcaattccaa caatggcaaa atcgagcgca tcggtcaaat ttattgtgtc aacggtaaag
    19201 aaaagcaaga ggtcgctcat ttacatgccg gcgatatcgg agccgtcgtt aaacttagat
    19261 tttcgaaaac cgcggatacc ttgtgcgatc agcgcgcacc gatcacttac ccgggaattt
    19321 catttcccga accagtcatt caaatcgcca tagagccgaa atcgcgtggc gacgaggaaa
    19381 aaatcagcgc cggtttacag tccttaacga gtgaggatcc aacaatgtcc tggcattacg
    19441 atcccgagct taagcagacg attttgtccg gtcaaggcga attacattta gccattattt
    19501 tacaacgttt aaaacaaaaa ttcggagttg atgtaaatca actgcctccc cgcatcccct
    19561 atcgagaaac tataaaaaaa cggggtgact ccaaatatcg tcataaaaaa caaactggcg
    19621 gtgcaggaca atttgctgag gtttggatgt acgtcgagcc aggtccgccc ggaagcggcg
    19681 tagaattcac caatacactt gttgggcaga atgttgatag ggtgtttgta ccttcggtcg
    19741 aaaaaggcgt tcgctcagcc tgtgaagaag gaattttagc cggatatcgt gtcaccgatg
    19801 tcaaagccgt attttacgat ggaaaaatgc atcctgtcga ttctaaggat attgctttcc
    19861 agatcgctgg caaaggcgct tttaaagaat gctttatgaa tggcaacccg gttctgctgg
    19921 aaccgattta taatctcgaa gtcattgtgc cagaagaata tatgggtgac gtaatgggtg
    19981 atatttcagt aaggcgtgga aaaatccagg gagttgattc tgaaggtcca tttcaaaaaa
    20041 ttaccgccaa agtacctctg gctgaactct atcgctatag cacggctcta cgctcgatga
    20101 cacaaggacg cggtatgcat cgtcagagtt tttcccatta cgaacaaatg cccaaagaaa
    20161 ttcaggataa agtcgtagcg gcttccagaa aagaacacga ggagtaaatt cggtggaatt
    20221 tgataaactc tgggcgccct ggcgaattgg ttatattacc aatcccaaat gtgaaggatg
    20281 ctttctttgc cggtatccat ccgaatctga tgataaaaaa aatctaatcc ttcagcgtgg
    20341 caaatcggca ttcgtaataa tgaattttta tccctataat aatggtcatc taatggtcgc
    20401 tccctatcag catacgaatg attttgccgc tttagatgat gctactcacc tcgaattatt
    20461 tcatttagtc gaaaagtcgg ttggtattct tcgtgaaact ctcaaagcgg aaggattcaa
    20521 tatcggtgta aatttgggaa cggtcgccgg cgctggtcta aaagatcacc tgcatataca
    20581 tattgttccg cgctggactg gcgatactaa cttcatgccg gttatcggac ataccaaagt
    20641 tataagtgaa ggattaactg aaacctggca aaaacttaaa tctaaattcg atgaatgatt
    20701 tttgatagtt gaatatcgct gttaaatgat ttaaatttgt attgcttttt tagattatga
    20761 atctggagta gtcccgc
//
LOCUS       DAOOMU010000034        20144 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2650_length_20144_cov_16.822191, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000034 DAOOMU010000000
VERSION     DAOOMU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 20144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20144
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2650_length_20144_cov_16.822191"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLI55_04800"
     CDS             complement(<1..440)
                     /locus_tag="PLI55_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPB00186.1"
                     /translation="MAEQQNIVKDGAGKEPKVKPTPNPVDKSVEKRLFILSSSPHIKA
                     STDVSSIMWLVVATLLPTVIFSIYNFGWRCLTVIATSMITAMIAEELFNLIRRRKITI
                     YDGSAALTGLLLALTLPPGLPLSMVIIGAAFSIIIGKQIFGGLG"
     gene            complement(484..1833)
                     /gene="rsxC"
                     /locus_tag="PLI55_04805"
     CDS             complement(484..1833)
                     /gene="rsxC"
                     /locus_tag="PLI55_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPB00187.1"
                     /translation="MKLKTFKKGVYPDEFKSLSDYKSIEKSPLPTEVFIPLQQHVGAA
                     CVPVVEKGMEVKTGQLIGKSGGFISSNIHASISGKVKTIEKLPHPLGNNSLMINIIGD
                     GHDEWVELPDSISDWENLTPEQINKIILDAGIVGLGGAAFPTQVKLNPPKNKTIDTFI
                     LNGCECEPYLTADHRIMVEHSEEVVLGLRIMMKALGVSRAIIGIEANKPDAIAALTKA
                     TEKFWGIIVQPLKVKYPQGAEKMLIDAVLNRKVPAGGLPMDVGVVVNNVGTALAVAEA
                     VTKHKPLIERVVTVTGQGVREPKNILARIGTPFQNLIDFCGGLTDDTVKVVMGGPMMG
                     VAQASLSVPVIKATSGIVCLTNKIAVERKEYPCIKCGACLHVCPMNLIPTRLARLSQF
                     AKWDEAEQYGIANCIECGSCAFICPASIPLVHHIRTGKTRVAEIQRQKRTEKKAVSV"
     gene            1992..2345
                     /gene="ssrA"
                     /locus_tag="PLI55_04810"
     tmRNA           1992..2345
                     /gene="ssrA"
                     /locus_tag="PLI55_04810"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2324..3598)
                     /locus_tag="PLI55_04815"
     CDS             complement(2324..3598)
                     /locus_tag="PLI55_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB00188.1"
                     /translation="MKPNKQLLIAHYTLPPAIGGVESILKPFAEVFARNGYLVTILSG
                     AGSIEGQNIKTSLLPDLNPNSPHVREIQRVLKFGSLPEFYEYQMQNLQRRIEAEIGNI
                     DTIIIHNIMTMPFNLMATEAFWNYILKYPNKKYYIWTHDLAWLIDEYKPNLYDRRPWS
                     LLRTEIPNVTYVTVSEYRKRQIAELLNIPRRKILVVPNTIKYQDFFKFEPATNIIVEA
                     LKIFSCYPIILVPARIIPRKNIERSIKVIAALRNTWPNLVGIITGMPKRENGALTEYA
                     ASLYNLVQENNLSNNLFFLGDLFERLNIPAEKNRAVVRDLYFISNLVLYLSMDEGFGL
                     PILEAGIARTPLALSQIPVFREVAKEGALYLPLDESPEYNANRIVKYLTDNQPRSDVL
                     YKSIIQKNNWDDLWESHLNSIFNESMSGGGGN"
     assembly_gap    3770..3869
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3870..4276)
                     /locus_tag="PLI55_04820"
     CDS             complement(<3870..4276)
                     /locus_tag="PLI55_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00189.1"
                     /translation="MPMFIKYYPNKYNRHQLIEDLNVIRDKGYNGVFYCINKPYETGD
                     LTMKFFPVARMIELTKLAGLKAGIILDCFQNPFLWQMDIFSPPVNYLGTAYAPQKGYY
                     PVCPNNPLGHDFYHLLLQKTYRLGQIDYCMFENL"
     gene            complement(4324..4399)
                     /locus_tag="PLI55_04825"
     tRNA            complement(4324..4399)
                     /locus_tag="PLI55_04825"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4361..4363),aa:Pro,seq:ggg)
     gene            complement(4459..4845)
                     /locus_tag="PLI55_04830"
     CDS             complement(4459..4845)
                     /locus_tag="PLI55_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPB00190.1"
                     /translation="MEKPIKKLYYSIGEVSEITGLKQYVLRYWEKEFPSLTPTKNRAG
                     NRAYREKDIQLIQYIKHLLYDKRFTIEGAKQKLKEMKPEDWQKAIDTENRNELDETVG
                     ERTDTAMVLSEIKKGLNELLEMLNNW"
     gene            complement(4929..5594)
                     /locus_tag="PLI55_04835"
     CDS             complement(4929..5594)
                     /locus_tag="PLI55_04835"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB00191.1"
                     /translation="MLNRGYEFATLSNLQANKNKPNLIALTFDDGYQDIYLYAFPILK
                     SLGIPATIFIITDYLDRPNTWDINLGGIHFNHLTYDNIAELIQNGWEIGSHGLSHHLL
                     VGMPTLEMQAEIHDSKKLLEERFNCEVRFFCAPFGKLNPKIISEAKSAGYQGICGFFP
                     FRYYHQKPPDFFIPRLAVYSFDSLNAVARKLATNWQLRREIIKQNVVNFCANGTIIVQ
                     KLR"
     gene            complement(5727..6980)
                     /locus_tag="PLI55_04840"
     CDS             complement(5727..6980)
                     /locus_tag="PLI55_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB00192.1"
                     /translation="MNQKIIPARRTEKVTYAIRDIAVKAKHLEDQGRKIIYLNIGDPP
                     VYDFETPFELRQIVSEKILQSSANSQLNVSVYGDSMGLIEAREAVARYATEKKGIKGV
                     TANDVFMANGASEAITLAIAALINPGDNIMTPSPGYPLYSGQIPVYHGNLNPYLLNES
                     TGWQIDFDELEKRVNKRTKAIVVINPNNPTGANYNRESLLNVINFARRHNLVIFADEI
                     YDKLLLDGQTHTSIASLADDVPVVTFGGLSKNYIMPGWRVGWAIFSDPTAVMADYREA
                     INKLLRARLCAVHPQQYMVAPALNGNDSHLPQVIQKLTIRRDITDKMLNEIPGISCVK
                     PEAAFYAFPRIQLPPGVTDEQFVLELLNDTGVLVVYGSGFGQQRGTNHFRVVFLPDEN
                     ILRHSYTLIGEFTAKFYRKYNFKPE"
     gene            complement(7020..8261)
                     /locus_tag="PLI55_04845"
     CDS             complement(7020..8261)
                     /locus_tag="PLI55_04845"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPB00193.1"
                     /translation="MISENIQRTILPNGIRVLSEYVDSVYSVSLGFWIQAGSQDEEDN
                     ENGIAHLLEHMVFKGTHKRSAFAIAHEIESLGGIINAFTSRNVTCFNVQLLHENLAKG
                     VEVLSDLILHGRLDPHELEKEKRVICEEIREIEDTPHDLIHDLFIQQLFPNQAMGRPI
                     QGTVENVNALQVSDLKRFLKRHYTTGRLVVSAAGRVNHEQLVKLVEKYCQDMGNGADY
                     QEKDSGITQPETQKIYPKPIMQSHVILGRRIFGQGDSRRFQLGLLNMILSGGMTSRLF
                     HNIREKYGYVYEVYSFSDLFLKDGLFGVYAGSDKNRLDMVIDKINNEMTALYRKPIPV
                     REYKKAKQQVKGGLVLAMENMNARMSNLAKTEIYEKHIITIAELLEIIDKISIEDLQE
                     LARYLFDRNAFIETIIQPIEP"
     gene            complement(8265..10460)
                     /gene="pnp"
                     /locus_tag="PLI55_04850"
     CDS             complement(8265..10460)
                     /gene="pnp"
                     /locus_tag="PLI55_04850"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPB00194.1"
                     /translation="MYQKKMTLGGRELSIETGRMAKQASGAVVVRYADTMILVTAVTN
                     FDAVAEQDFFPLQVEYRERAYAAGKIPGGFFKREGRPSEKEILGSRLIDRPIRPLFPE
                     DFHCDTQIIANVISSDQENPADVLGIIGASTALMISEIPFSGPIAGVRVGRVNGEFIL
                     NPTIKQMEEGELDIVVAGTEDSVVMVEGECKIISEDLLIEAIEFGHSAVRELIQFQKD
                     IVAELKPVKRPIEPVEEPLELKNAVSAQTETMLADWRQLAQVSKKSWNDAIRTFVNKL
                     TEEYAEQYPEKGNYIAQVVDEIVKKDMRRLIIETGKRLDGRALDQIRPITCEIGILPR
                     AHGSALFTRGETQSLGSVTLGTKLDEQIIDDIDREEFTKKFMLHYNFPPFSVGEVKPM
                     RGTGRREIGHGNLAERALKTQIPSEEEFPYTIRIVSDILESNGSSSMATVCAGSLAMM
                     DAGIPVKETVAGIAMGLIKEDNKLIVLSDIIGAEDHYGDMDFKITGTRTGITAIQMDL
                     KIQGIAASTMRGALEQARVGRMQIIDIMEQTISKPREKISDFAPRMLTLKVDNDLIGE
                     IIGPGGKNIRALQTESNSKIEIDDNGMVFISAADFASAEKARQLIIDMIAKPEEGKIY
                     HDCPIKRLESYGAFVEFLPGKEGLLHVSEIAWRRTEDINSVLKVGDKVDVLLKKIAAP
                     GRFELSMKELVEKPEGYQETPRNSHQPRQSHSDRSKGDHHSSHRTHPRY"
     gene            complement(10479..10748)
                     /gene="rpsO"
                     /locus_tag="PLI55_04855"
     CDS             complement(10479..10748)
                     /gene="rpsO"
                     /locus_tag="PLI55_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPB00195.1"
                     /translation="MTLTKEIKSELVSKYGKNSHDTGAITAQIAMLTERIKDLSQHFA
                     AHPKDHSSRRGLMKMVSRRKHLLEYLRRKDNAAYKELIEKLELRK"
     gene            complement(10745..11713)
                     /locus_tag="PLI55_04860"
     CDS             complement(10745..11713)
                     /locus_tag="PLI55_04860"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPB00196.1"
                     /translation="MNIYYGIENIPADYKGACVTLGVFDGLHLAHRQIIQKVIELATE
                     QNSRAMLITFEPHPRRILSQSSANHLPILTTVDEKIELLEKIGIEEVLFIKADRTFLN
                     TEESVFVQEILVKKLKVSAVVVGYDYHYGRDRKGNPQTLTAAGKKFGFKVVVIPPFAK
                     NEILVRSSLIRELITEGKILTANQMLGWQYSIKGKVVRGTGRGHKLGFPTANLVVNPP
                     DKLIPGDGVYFVKACLSNFEYFGILNIGMRKTFNESERVIELFIINFKDEDLYHREIK
                     VIFIERLRDELKFETSEALKKQMQADLEICLAKINKLENKTEVCFK"
     gene            complement(11710..12441)
                     /gene="truB"
                     /locus_tag="PLI55_04865"
     CDS             complement(11710..12441)
                     /gene="truB"
                     /locus_tag="PLI55_04865"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPB00197.1"
                     /translation="MPRISKSYSKKYINRKRIIKTDWSNKILNVYKPAGISSYQVVRR
                     VKNLLKIKKVGHSGTLDPFAEGVLLILIGGATKKMNDLLKLPKSYEAILRLGEMTASG
                     DNTAPIIKTAAIQSITAENLIEITKNFIGVIEQIPPIYSAKKINGRPSYKYARKGQEI
                     KLQPTQVNIYELDLRLSDYNSIFIKVTCSSGTYIRKLGEDLAEALGTVGHLTNLKRTR
                     IGNYDWKEAVPYSELNGYLLENATV"
     gene            complement(12368..12742)
                     /gene="rbfA"
                     /locus_tag="PLI55_04870"
     CDS             complement(12368..12742)
                     /gene="rbfA"
                     /locus_tag="PLI55_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPB00198.1"
                     /translation="MTGYRIKRVEDEIKRIVAEIFIRDIRLDNVGLITVTKVVCSSDL
                     REAKIYMSIYNNQKYSIAEAMKIIKSHTSFVRGLLGNRIMMRFVPRITFYYDETQEYA
                     ENIEKLFQKIHQPEEDNQNGLE"
     gene            complement(12742..13023)
                     /locus_tag="PLI55_04875"
     CDS             complement(12742..13023)
                     /locus_tag="PLI55_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPB00199.1"
                     /translation="MTIGLLQIELRLKNTHSLKDKRSIINRIKNQVRAKFNVSIAEIE
                     PNDQYGHCLLGITTISNDQKIVHQTLNSVEYLVENFAEIQIIGREMEMF"
     gene            complement(13040..15568)
                     /gene="infB"
                     /locus_tag="PLI55_04880"
     CDS             complement(13040..15568)
                     /gene="infB"
                     /locus_tag="PLI55_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPB00200.1"
                     /translation="MSIDPKDKPKRIFEIAKELNISHNDIIKYLEKEGIPCKSIMTPV
                     DESSYMKILGEFAKEKDIVERLRLERARREAENRRKAEEDARRTAEMERRKLEEEVYK
                     SALLFFNSAIKDVFKYSLNVQSQLIESVWETAAVTDRLPEIPKEPEITEIAEPSIEKT
                     TTTTPPLESPKKKGKKKLRRIKIDELETRLDLKHGKHKPTETGDEVSHRRKKPGNERE
                     ANDRVVDAQIRKTMAKMDEKSTKKRHKKTETVAEESEVSKKIRISDFATVDSLASLIG
                     IDAADVIQKCIELGTFVTVNQRLDFDTISVLCDEFGFEVEKMEHYGEDQIKIEDTKED
                     LENATPRPPVVVIMGHVDHGKTSLLDYIRRSNVVAGESGGITQHIGAYKVELPNKKFI
                     TFLDTPGHEAFTAMRARGAQVTDIVVLVVAADDGVMPQTIEAINHAKAANVPIVVAIN
                     KIDKPEADVERVKRDLANYGVLVENWGGKVQTAEISAKTGKGIDHLLELILLEAEMLE
                     LKANYQTLAKGIVIESSIDKRRGPMATVIIKKGTLKIGDPFVCGAAFGKVRTILDERS
                     QKLTKAEPSDPVVISGFDFPPKPADIFAVVQDEREARKIATERQKIEHEHQKRQVKEW
                     SLDTISAQIAEGKIKQLNVILKGDFDGTVEAIGQTLQDLGNEEVAVNIIHKAAGNITE
                     SDVLLAKTSQAIVIGFNVSANPKVNDLAKKENVEIRHYSIIYELTDDIKAALEGLLTP
                     EKIEEHLGEAEVREIFKIPRVGEVAGSYVIEGKVTRNATARLKRDGEVIFEGAVESLK
                     RFKDDVREVAEGFECGISLQGAPEFKVGDRIEFFEVKSVKRTLA"
     gene            complement(15573..16964)
                     /gene="nusA"
                     /locus_tag="PLI55_04885"
     CDS             complement(15573..16964)
                     /gene="nusA"
                     /locus_tag="PLI55_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPB00201.1"
                     /translation="MVNREIIEAFSQIAREKNVDRNELGDIIEKVFLTLIEKKYGSTE
                     NFDVIVNMDKGEIEIYHQMTIVDNPTGSPHEITLENARKTEPDLQVGDPFIEVIDPMS
                     FGRRLISHAKQILNQQIVRVEKNQIYEEYAKKIGQIVIGDVHQIRRDAIFILIGKAEL
                     KMPREEQIPNERYRRGENIKAIIKEAIMGPSGPEIIVSRADKSFLVKLFENEVPEIYD
                     KTIEIKKVARHAGDRSKVIVESNDRRIDAVGACVGIKGSRIQAIVRELNNEKIDIINY
                     SSEPEILITRALSPAKPLELEIDEEKKTALAIFADDEISVAIGKMGQNIRLASEVTGY
                     TIDAIKKSDYELTESETLYLEDIEGITEHNWKLLYSYGIETAEEFLDTPREELLSIKG
                     LGEKTLDKLEPIIIEAQEKRKAEVATIHEAAKAQREAALQEAQENKEKQTVEEETDLS
                     DEDIIDDFGQEEK"
     gene            complement(16987..17445)
                     /locus_tag="PLI55_04890"
     CDS             complement(16987..17445)
                     /locus_tag="PLI55_04890"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00202.1"
                     /translation="MELETTKNAARAIVDKIVERFDAYVVGFKVNRQGYRTFVKVLIT
                     TDHGIALDQIAEISRAVRDDVAFNELFPDNFQLEVSSPGIDFPLRNFRDFHRNLGREM
                     NVFHHSENLKSPLKGKLESVDEDQLVIANAEGTHTLKMGEIDFAKVIIQW"
     gene            complement(17531..18226)
                     /locus_tag="PLI55_04895"
     CDS             complement(17531..18226)
                     /locus_tag="PLI55_04895"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00203.1"
                     /translation="MLKPKKKITRKELKKDPVLEKISQIENLVRKQRKLVSYILLGVV
                     VVVLLTILLVNSKKKANTAAMGELGLAEMSFARNDYDDAILRLENLIEKYSRTRSSGM
                     ATILLARCYLAKDDYENARVNFEKYIDDYKDDKFLTAGAYSGLAICAEQDSNFALAAE
                     YFEKGGKISPHKFHKHELLLNSARNYLKVNNLEKAEEIVNIILNDEPESVNKNSAELL
                     LAQITTRKGLALK"
     gene            complement(18255..18327)
                     /locus_tag="PLI55_04900"
     tRNA            complement(18255..18327)
                     /locus_tag="PLI55_04900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18291..18293),aa:Arg,seq:cct)
     gene            complement(18467..19621)
                     /locus_tag="PLI55_04905"
     CDS             complement(18467..19621)
                     /locus_tag="PLI55_04905"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HPB00204.1"
                     /translation="MNVTKRFLRDEELAVYLQQLDIKLEKVDDNYRAEALILHQNDPE
                     KGYPKLIKNMQIYSQVAITELIVKTIPVHVMLNKTRRPSLVSSSFGENTDTIKKTCEL
                     CKLDRGQRGIIIKVGNEQEFIILTNPGITYPGDLTIAALKHDKQLITGRFNDMIQISR
                     ALYHFSIYFNGILAGASSPHFHFQAGYKDKLIGEIQIQKLLAGQSVGQARLKRILKSG
                     DLEVFYIENFLRPVHIVVTKNAEALLKFFDHYLILLKDVSQNIKGIKNVPDYGSLIPA
                     LGMRENEPRLNIMLKYFPEYGGFILALFPKRYNRPSCYFRKGNSQILIGMGIKEALGN
                     IITGRETDYKRLRNNPALISQIYTETSISPESVEILNQNLKKINILPRNR"
BASE COUNT         5483 a         4293 c         3782 g         6486 t
ORIGIN      
        1 tgcccaaggc caccgaaaat ttgtttaccg attataatgg aaaaagcggc accgattatg
       61 accatactaa gcggtaaacc cggtggcaag gtcaaagcca gcaataagcc ggtcaaggcg
      121 gcactaccat cataaattgt aattttacga cgtctaatta aattaaataa ttcctcggca
      181 atcatagccg taatcataga agtggctata actgtcagac aacgccatcc gaaattgtaa
      241 atactgaaaa taaccgttgg cagtaaagta gccacaacca gccacataat tgaagaaaca
      301 tcagtagagg ctttaatatg gggcgaagat gaaagaataa aaagtcgttt ctcaactgac
      361 ttgtcaacag gatttggagt cggttttact ttcggctctt ttccggctcc atctttaact
      421 atattctgct gttctgccat tatgataaat ttccttatta aatattcgat attgaacgat
      481 atttcatacg cttacggctt ttttttcagt tctcttttga cgctgaattt cggctacccg
      541 cgttttccca gttcggatat gatgaactaa aggaattgat gctgggcaaa taaaagcgca
      601 agaaccacat tcgatacaat tagctatacc atattgttcg gcttcatccc acttggcaaa
      661 ctgcgataag cgcgccagcc tggttgggat tagattcatt ggacagacat ggaggcaagc
      721 cccacatttg atacagggat attctttcct ttcgacagcg attttattcg ttaaacagac
      781 aattccactg gtagctttta taacgggcac agataaagaa gcctgagcta cgcccatcat
      841 tggaccgccc atcactacct tgaccgtatc gtcggtcagt ccgccacaga agtcaattaa
      901 attctgaaat ggagtgccga ttctggctaa tatatttttt ggttcgcgca ctccctgacc
      961 ggtaaccgta acgactcttt caatcagtgg tttgtgtttt gtaacggctt cagccacagc
     1021 cagagccgtg ccgacattat taacaacaac gcccacgtcc attggtaatc cgccggcggg
     1081 tactttgcgg tttaaaacgg catctattaa catcttctcg gcaccttggg gatattttac
     1141 cttcagaggc tggacaataa taccccagaa tttttcggta gctttggtta gcgccgcaat
     1201 agcatccggt ttattggctt cgatacctat tattgcccga ctgactccga gcgctttcat
     1261 cataatgcga agtcccagga ctacctcttc cgaatgttcg accataatgc ggtgatcggc
     1321 tgtcaaataa ggttcacatt cacaaccgtt aaggataaaa gtatcaatgg ttttattttt
     1381 gggcggattt aatttcactt gcgttggaaa agccgctccg cctaagccaa cgattccggc
     1441 gtctaatata attttattga tttgttcggg agtaagattt tcccaatcgc taatcgaatc
     1501 aggtaattct acccattcgt cgtgaccatc gccgatgatg tttatcatta gcgaattatt
     1561 tcccaaaggg tgcggtaatt tttcaatcgt ttttactttt cccgaaattg aggcgtggat
     1621 attgcttgat ataaatccgc cgcttttgcc gatcaattga ccggttttaa cttccatacc
     1681 tttttcgact accggtacac atgcggctcc aacatgttgc tgaagcggaa taaacacttc
     1741 cgtcggtaaa ggcgattttt ctatgctttt ataatcactt aaacttttaa actcatccgg
     1801 ataaacgccc ttcttgaacg tcttcaattt cattttaatt ttgctcctac tctaatcaag
     1861 ctctaaaagt caaaaaatcg aggaaattta atcaaaatat tccaaatatt ccttaaataa
     1921 tttggaaaga tcctgacatt cgatagtttg tttataaagg agaaagtgtg taaatttcta
     1981 tgctttttga cgggggcgaa tggattcgac gggattgaag atctgccgaa tagcgtgtcg
     2041 tggttttcag gagggccacg ttaataaacc tgagaatcga aaatgccaat tcttttggca
     2101 ctgtcgctta cgcttaagta agtgaccgtt ctgggccggt ctcgcctgca ggctggttca
     2161 gagcgtcgtc aaagcaggct cgttccgttt atcggtttgc gtaaacggaa ttaaaaacag
     2221 caaactggct tttaccaagt ttgtccgctg gcacggtaaa agcgagattc agcaacggac
     2281 tacacacgta gcgattctgc agttctcttt ttcggacggg ggttcaattc cccccgcctc
     2341 cactcatact ttcgttaaag atggaattta gatggctctc ccagagatca tcccaattat
     2401 ttttttgaat gatagattta tataatacat cgctccgcgg ttgattatct gtcaaatatt
     2461 ttactatgcg gttagcatta tattccggac tctcatccag cggcaagtat agagcacctt
     2521 ctttcgctac ttctctaaat accggtattt gactgagtgc caatggagtt ctggcgatac
     2581 ctgcttcaag aattggtaaa ccgaaacctt catccatgct taaataaagc actaaattac
     2641 tgataaaata aaggtcacga accacagctc gattcttttc tgccgggata tttaatctct
     2701 cgaaaagatc gccgagaaaa aacaggttat tggacaggtt gttttcttga accaaattat
     2761 aaagactggc ggcgtattct gtcaaagcgc cattctccct cttaggcatt ccggtaatga
     2821 taccgacaag attaggccag gtattccgca gtgccgcaat tactttaata ctgcgttcaa
     2881 tattcttcct tggaattatt ctggctggga ccaaaatgat tggataacaa ctaaaaatct
     2941 tgagtgcttc aacgattatg ttagttgctg gttcaaattt gaagaaatcc tggtatttaa
     3001 tcgtattagg aactacaaga atttttcgac gtggaatatt taacaactcg gctatttgcc
     3061 gtttacggta ttcagaaaca gtaacgtaag taacattggg tatttcggtt cgaagtagtg
     3121 accacggccg tcggtcgtat aaattaggtt tgtactcatc aatcaaccat gccagatcat
     3181 gagtccagat gtaatacttt ttatttggat attttaagat gtaattccaa aaagcttcgg
     3241 tagccatcag attaaaaggc atagtcatta tattgtgaat aataatggta tctatattac
     3301 cgatttcggc ttcgatccgc cgctgaaggt tttgcatttg atattcgtaa aattcaggta
     3361 gtgaaccgaa ttttaaaacc cgctgaattt cacgaacatg cggactattc gggttcaaat
     3421 ccggcaatag actggtcttg atattctggc cttcgattga accggctcca cttaggattg
     3481 ttactaaata gccattgcgc gcaaatactt cggcgaacgg cttcaggata ctctcaaccc
     3541 ctcctatagc cggtggtaag gtataatggg caatcagtaa ttgtttatta ggcttcacaa
     3601 gaggtttact aaatttgctc cggatttcat tcgtaagaag aattaatttt cgtattttaa
     3661 tgattgccag ttatcaaaca gaagaccggc aaaattttca gtcgagtcaa tcgctaaata
     3721 ttgcttaaga tcactttcgc tttgaacttt aaaagtcggg aaaagtttan nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gattttcaaa catacagtaa tcaatttgcc
     3901 ccagacgata agtcttttga agcaataaat gataaaaatc atgtccaagg ggattgttgg
     3961 gacaaaccgg ataatatcct ttctgggggg cataggcagt cccgagataa ttaaccggtg
     4021 gagaaaaaat atccatttgc cacaaaaatg gattttggaa acaatctaaa ataattccag
     4081 cttttaagcc cgccagttta gtcagttcga tcatccttgc caccggaaaa aatttcatcg
     4141 tcaaatcacc ggtttcataa ggcttgttta tacaataaaa cacgccatta tatcctttat
     4201 cacggataac atttaagtct tcaatcagtt ggtgacggtt gtatttattg ggataatatt
     4261 tgataaacat tggcatttta atctgaattt cgtgaaaaat ttatttaatt caatcgaaat
     4321 ttgtcggagc gagtggattt gaaccaccga ccccctgaac cccattcagg tgcgctaatc
     4381 cggactgcgc tacgctccgc ttgtcaaaaa cgtcagtaat ttaattgata agaatttatt
     4441 tatctacgaa aaattggatt accagttatt aagcatttcc agtaattcgt tgagcccctt
     4501 tttaatttcc gataatacca tagcggtatc ggttctctcc cctactgttt catccaattc
     4561 gttcctattc tcagtatcaa tagctttttg ccaatcttct ggtttcattt ctttcagttt
     4621 ctgtttagcg ccttctatag taaaacgttt atcatacaat aggtgtttga tatattgaat
     4681 taattggatg tctttttcac gataagcgcg gttgcccgcc cggtttttag tcggtgtcaa
     4741 tgatggaaat tctttctccc agtatcggag aacatattgc ttgagcccgg taatttcact
     4801 gacttctcca attgagtaat ataatttttt aattggcttt tccatatcac acctgttatc
     4861 actattattt aaaccattac tttaatagta taatcagttt aaagggttat gtcaagttat
     4921 ttttttattt atcgcaattt ctggacgata atagtgccgt tggcgcagaa gtttacgacg
     4981 ttttgtttga taatttcacg gcgcaattgc caattggtag ccagtttcct tgccacagca
     5041 tttaatgaat caaatgaata aactgccaga cgtggaatga aaaaatcagg aggtttttga
     5101 tgatagtatc taaatgggaa gaatccgcaa ataccttgat aaccagcaga tttggcttca
     5161 gaaataattt ttgggttcaa tttcccgaaa ggagcacaaa agaatctaac ttcgcaattg
     5221 aatcgttctt caagtaactt ctttgagtcg tgtatttcgg cttgcatttc taaagtcggc
     5281 attccgacta atagatgatg tgataagcca tgcgagccga tttcccaccc attttggatt
     5341 aattctgcga tgttatcata tgtcagatgg ttgaaatgga ttccacctag attaatgtcc
     5401 caggtatttg gccgatctaa ataatcggtg atgataaaaa tagttgccgg gatacccaga
     5461 ctttttaata tcggaaaggc atataaataa atatcctgat aaccatcgtc gaaggtcaga
     5521 gcgatcagat taggtttatt tttatttgcc tgaagattgc taagagtggc aaattcataa
     5581 ccacgattaa gcaaaaactc gatatgtttt tgaaattttt ctggtttgat gcaactcgcg
     5641 ccgatttccg aacgccaatc aattttatga tagacaagaa ctcgattctt tatgggaaca
     5701 aactcgtcta tggctccacc ctttgactac tccggtttga aattatattt gcggtagaat
     5761 tttgccgtaa attcgccgat tagcgtataa gaatgacgca gaatattttc atctggtaga
     5821 aaaactaccc gaaaatgatt agtccctcgt tgctgaccga aaccgctacc gtaaactact
     5881 agtactccgg tatcattgag caattcgagg acaaattgtt catcagtaac gccgggcggc
     5941 aattgaatac ggggaaaagc gtaaaaagcc gcctcgggtt taacgcaaga aatgccagga
     6001 atttcattta acattttgtc tgtaatatca cggcgaatcg tcagtttttg aatgacttgg
     6061 ggtaaatgag agtcattacc attcagggct ggagccacca tatattgttg cggatgtacc
     6121 gcacaaagtc ttgcccgcaa gagtttatta atggcttcgc ggtaatctgc catcacagcg
     6181 gtaggatcag aaaatattgc ccaaccaacc cgccaaccag gcatgatata attcttactc
     6241 aatccaccaa aggtaactac gggtacatca tcagctaatg aggcaattga ggtgtgcgtt
     6301 tgaccgtcaa gaagcaattt atcataaatt tcgtcagcga aaatcactaa gttatggcga
     6361 cgggcaaaat tgatgacatt taataaactt tcgcgattat aattcgcacc agtcggattg
     6421 ttaggattaa tgacgacaat tgccttagtg cgcttgttga cccgcttttc taattcgtca
     6481 aaatctatct gccagccggt tgattcattt aataaatatg gatttaagtt accatgataa
     6541 accgggattt ggccgctata aagtggatag ccaggcgagg gcgtcatgat attgtcgccg
     6601 ggattaatta aagccgctat cgccagcgta atggcttccg aagctccatt agccataaag
     6661 acatcattgg ctgttacccc ttttattcct tttttttcag tagcatagcg agcaactgct
     6721 tctctggctt cgattaaacc catcgaatcg ccatagaccg agacattcaa ttgggaattg
     6781 gcactcgatt gtaaaatttt ttcacttaca atctggcgca gttcaaaggg agtttcaaag
     6841 tcataaactg gaggatcgcc aatatttaag tatataattt ttctgccctg gtcttctaag
     6901 tgcttggctt tcacagctat gtcacggatc gcataagtga ctttttcggt acgccttgct
     6961 ggaatgattt tctgattcat ataattttct cttttatgac tttttgccat ttaacgtaat
     7021 cagggttcta tcggctgaat aatggtttca ataaaggcgt tccggtcgaa taagtagcgt
     7081 gccaattcct gtaaatcctc aatgctgatt ttatcaataa tttccaataa ttcggcaatc
     7141 gtaataatat gtttttcgta aatttctgtc ttagctaaat tactcattcg agcgttcata
     7201 ttttccatcg ccagtaccag tccacccttt acttgctgtt tggctttttt atattcacga
     7261 acgggaattg gtttacgata aagcgcggtc atttcattat taattttatc aataaccata
     7321 tccagtcggt ttttatcaga accagcatat actccgaata atccatcttt caaaaaaagg
     7381 tctgaaaatg agtaaacctc ataaacgtaa ccatattttt cacgaatgtt atgaaataaa
     7441 cgcgatgtca ttccgccact taaaatcata ttgagtaaac cgagctggaa tcgtcgcgaa
     7501 tcgccttgtc cgaaaatccg gcgtcccaaa ataacatggc tttgcattat cggtttggga
     7561 taaattttct gggtttctgg ttgagtaata ccggaatctt tttcttgata atcggcacca
     7621 tttcccatat cttgacaata tttctcaacc agttttacta attgttcatg attgactctt
     7681 ccggcggctg atacaactaa acgaccggtc gtataatgac gcttaaggaa acgttttaaa
     7741 tcagacacct gaagggcgtt gacattttcg actgttccct ggatcggacg ccccattgct
     7801 tgatttggga aaagctgctg aataaataaa tcgtggatta agtcatgtgg agtatcctcg
     7861 atttcgcgga tctcttcgca aataactctt ttttccttct cgagttcgtg gggatcaagc
     7921 cgaccatgca aaataaggtc agataacacc tcaacacctt tagcaagatt ttcatgtaac
     7981 aactgcacat taaagcaagt cacattccgg ctggtaaagg cattgatgat tccacccagc
     8041 gattcaattt catgcgcaat agcaaaagct gaacgtttat gcgttccctt aaaaaccata
     8101 tgttcaagca aatgcgcaat accgttttca ttgtcttctt cgtcttgaga accagcttgt
     8161 atccagaatc ctaacgaaac tgaataaacc gaatcaacat attcagatag gactcggatt
     8221 ccgttaggta gaattgtacg ctggatattt tcggaaatca tcgcttaata gcgggggtgt
     8281 gtccgatggg atgaatgatg atcacctttt gaacgatcac tgtggctttg ccttggttgg
     8341 tgagaattac gtggcgtttc ttgataacct tccggctttt caaccaattc tttcatcgaa
     8401 agttcaaaac gtccgggggc ggcgattttt ttcaatagaa catctacctt atcgccgact
     8461 ttcaagacgc tattaatatc ctcagttcga cgccaggcaa tttcagaaac atgcaacaga
     8521 ccttctttac caggtaaaaa ttcaacgaag gcaccgtaac tttccagcct ttttatcgga
     8581 cagtcatgat aaatcttgcc ttcttccggt ttagcgatca tatcaataat caactggcgt
     8641 gctttttcag ccgatgcaaa atctgcggct gaaatgaaaa ccataccgtt atcatcaatc
     8701 tcaatttttg aattactttc agtttgcaaa gcccgaatat tcttaccgcc gggaccgatt
     8761 atttcgccta ttaaatcgtt atcaactttt aaggtcaaca tacgcggtgc gaaatcggat
     8821 attttttctc gcggtttgct gattgtctgt tccataatat caataatttg catgcgaccg
     8881 acacgagctt gttcgagggc gccacgcata gtcgaagccg caattccctg aattttcaga
     8941 tccatctgaa ttgcggtaat gccggtacga gtaccggtta ttttgaaatc catatcgccg
     9001 taatggtctt ccgcgccaat gatatccgat aatactatga gtttattatc ttctttaatt
     9061 aatcccatcg cgataccagc taccgtttct ttaaccggaa ttccggcatc catcatagct
     9121 agcgaaccgg cacaaaccgt tgccattgaa gacgagccat tcgattccag aatatcggaa
     9181 acgatccgga ttgtataggg aaattcctcc tcgcttggaa tttgagtttt tagagctctc
     9241 tcagccaaat taccgtgtcc aatttcccgt cggcctgtgc ctctcattgg tttgacttca
     9301 ccaacgctaa atggcggaaa attgtaatga agcataaatt tcttagtgaa ttcttcacgg
     9361 tcaatatcgt caatgatttg ctcatccaat ttcgttccga gagttaccga acccagactt
     9421 tgagtttcac cgcgagtaaa aagggctgag ccgtgggctc gtggcaaaat tcctatttca
     9481 caagtaatcg gtcgaatttg atccagtgcg cgaccgtcga gtcttttgcc ggtctcgatt
     9541 atcagccgcc gcatatcttt cttaacgatt tcatcaacta cctgggcgat gtaattgcct
     9601 ttctccggat attgttcggc atattcttct gtcaatttat tgacgaaagt tcgaatggca
     9661 tcattccagg attttttgga aacctgcgcc aattgacgcc agtcggctag cattgtttcc
     9721 gtttgagcgc taaccgcgtt ttttaattcc agcggttctt caaccggttc aatcggccgt
     9781 ttgactggct ttaattccgc gactatatct ttctgaaact gaataagttc ccgaacggca
     9841 gaatgtccga actcaatagc ttcaattaac aaatcttcgg aaattatttt acattcacct
     9901 tcgaccatta caaccgaatc ttcggtacca gccacaacga tatcgagttc accttcttcc
     9961 atctgcttga tagtgggatt gagaataaat tctccattca cccgaccaac tcgtactccg
    10021 gcaattggac cggaaaatgg aatttctgaa atcatcaaag ctgtcgaagc tccaataata
    10081 ccgagtacat cagcgggatt ttcctgatcg cttgagataa cgttcgcaat tatctgagta
    10141 tcacagtgaa aatcttccgg aaatagaggt cgaatcggtc ggtctatcaa ccgtgaaccc
    10201 agaatttctt tttccgatgg acgtccttcg cgtttgaaaa aaccgccagg aattttacca
    10261 gcggcatagg ctcgctcccg atattctacc tgtagaggga agaaatcttg ttcggcaacg
    10321 gcatcaaaat tggttaccgc tgtcactaaa atcatcgtat cggcataacg aacaaccacg
    10381 gctcccgaag cctgtttagc cattcggccg gtctcaatac ttaattcacg acctccaagg
    10441 gtcatctttt tttggtacaa ttatttactc ccaattattt attttcttaa ttcaagtttc
    10501 tcaattaatt ctttataagc cgcattgtct ttacgacgca aatattccaa aagatgcttc
    10561 cgacggctaa ccattttcat caggccgcgc cgcgaactat gatctttagg atgggcagca
    10621 aaatgctggc ttaaatcttt gatcctttcg gttagcatag caatctgggc tgtaatcgct
    10681 ccagtatcat ggctgttttt cccgtacttg gatactaatt cacttttgat ttcttttgtc
    10741 aatgtcattt aaaacatacc tccgttttat tttctaattt attaattttc gctaaacata
    10801 tttccaaatc agcttgcatc tgttttttta gtgcctcact cgtttcaaat tttaattcat
    10861 cgcggagtct ttcgataaaa ataaccttaa tttcacgatg atacaaatct tcatccttaa
    10921 aatttattat aaataattca attacccgct cagattcgtt gaaagtcttt cgcatcccaa
    10981 tattcaaaat tccaaaatat tcgaaatttg aaaggcaagc tttaacaaaa tagactccat
    11041 cacctgggat taatttatcc ggtggattta caacaagatt agcggttgga aaaccgagtt
    11101 tgtgaccacg acccgttcct ctaacaacct ttcctttaat actatattgc cagccgagca
    11161 tttgattggc agtaaggatt ttcccttcgg ttataagttc tcggatcaac gatgaacgta
    11221 ccaaaatctc gttctttgca aacgggggta taactaccac cttaaaaccg aactttttgc
    11281 ccgccgcagt taaagtctgg ggattaccct ttcgatcccg accataatga taatcatacc
    11341 ctacaacgac agcgctcacc tttaactttt taactaaaat ttcttgcacg aataccgatt
    11401 cttcagtatt tagaaaagtt cgatcggctt taataaaaag gacttcttct attccaattt
    11461 tttcaagtaa ttctattttt tcatccactg ttgtcaatat cggtagatga ttagccgacg
    11521 attgactaag tatccgacgc ggatgaggtt caaaggtaat taacatagct ctgctatttt
    11581 gctcggtggc aagttcgata accttctgaa taatctggcg atgcgccagg tgcaaaccgt
    11641 caaacactcc caacgttaca caagcgcctt tataatcggc aggaatattt tcaatgccgt
    11701 aataaatatt cataccgtag cgttctccag caaatatcca tttaattccg agtagggcac
    11761 ggcttctttc cagtcataat tgccgatccg ggtgcgcttc aggttggtca aatgacccac
    11821 cgtgccgagc gcctctgcaa gatcttcgcc tagtttacga atataggttc cactcgaaca
    11881 ggtaacttta ataaaaatcg agttataatc tgataatctc aaatccaatt cataaatatt
    11941 tacttgagtt ggttgcagtt taatttcctg tccttttcgg gcatatttat aagacggacg
    12001 gccattgatt ttcttggcgg aataaattgg tggaatttgt tcgattacgc cgataaaatt
    12061 tttggtaatt tcaatcaaat tttccgctgt tatagattga atcgcggcgg tcttaataat
    12121 cggagcagta ttgtcaccag aggcagtcat ttcacccaaa cgtaaaatcg cctcgtaaga
    12181 cttgggcaat ttcaataaat cattcatttt tttggttgcc ccgccaatta aaattaggag
    12241 cactccttcc gcgaacggat cgagtgtccc gctatgaccg acttttttta ttttaagaag
    12301 gtttttaaca cgacgtacta cctgatagga agaaatacca gccggtttat aaacgtttaa
    12361 aattttatta ctccagtccg ttttgattat cctcttccgg ttgatgtatt ttttggaata
    12421 acttttcgat attctcggca tattcttgag tctcgtcata atagaaggta atgcgtggta
    12481 cgaaacgcat cataattcga ttaccgagca gaccacgcac gaaggaagta tgactcttga
    12541 taattttcat tgcttctgca atcgagtatt tttggttatt gtaaatgctc atatagattt
    12601 tggcttctct tagatcagag gagcaaacaa ctttcgttac ggttattaag ccaacattat
    12661 cgagccgaat atcccgaatg aaaatctcag ccacgatgcg tttaatttca tcctcaactc
    12721 gttttatgcg ataaccagtc attagaacat ctccatttcc ctgccaataa tctggatttc
    12781 ggcaaaattc tcaaccagat attcgacaga gtttaaagtt tgatgcacta tcttttgatc
    12841 attactgatg gttgttattc cgagcagaca gtgaccatat tgatcatttg gttcaatttc
    12901 cgcaatggag acattgaact ttgctcttac ttggttttta atgcgattga taattgaacg
    12961 tttatccttt aaagaatggg tatttttcag gcgcagttct atttgtaaca atccaatcgt
    13021 cataacaatt tacgcccatt taagccagcg tccttttgac cgatttaact tcaaaaaatt
    13081 ctatgcgatc gccgacctta aattcgggtg ctccttgcag tgaaatgcca cattcgaaac
    13141 cttctgcgac ttctctgaca tcgtctttaa atcgtttcaa cgattcgaca gcaccttcga
    13201 aaataacctc tccgtcgcgt tttaaacggg ctgtggcgtt gcgggtaacc ttgccctcga
    13261 ttacataaga accggctacc tctccaaccc gcggtatttt aaatatttcg cgcacctcgg
    13321 cttcaccgag atgttcttca attttttcgg gagtcaataa gccttccagt gcggctttga
    13381 tatcatcagt caattcataa ataatcgaat aatggcgaat ttcgacattt tctttcttag
    13441 ctaaatcatt gacttttgga ttcgcgctta cattaaatcc aattacgata gcctgggaag
    13501 ttttggcaag cagtacatcc gattcagtaa tattaccggc ggctttatga ataatgttca
    13561 cggcaacctc ttcattccca agatcctgca aagtttggcc tatcgcctct actgtaccat
    13621 cgaaatcacc ttttagaatc acattgagct gttttatttt tccctcagca atctgagcgg
    13681 aaatagtatc taatgaccac tcttttacct ggcgtttctg gtgttcatgt tcaatttttt
    13741 gcctttcggt agcaattttt cgtgcttcac gttcatcctg aaccactgca aatatatcag
    13801 ccggtttagg gggaaagtcg aaaccagaaa tcacaaccgg atcggatggt tcggctttgg
    13861 tcaatttctg gctacgttca tctaagattg ttcgtacttt tccaaaggcg gctccacaaa
    13921 cgaatgggtc accgattttt aatgtcccct tttttattat tacagttgcc atggggccac
    13981 ggcgtttatc aatgctggat tcgattacaa tgcctttagc caatgtctgg taattggctt
    14041 tgagttccag catctcagct tctaacagaa ttaattctaa taagtggtct attccctttc
    14101 ccgttttagc tgatatttcg gctgtctgga ctttcccgcc ccagttttct accaaaacgc
    14161 cataattggc aagatcacgt ttgactcgtt cgacatcggc ttctggttta tcaattttat
    14221 tgatggctac tacgattggt acattggcgg cttttgcgtg attgattgcc tcgattgttt
    14281 gcggcataac gccgtcgtcg gcggcaacta ccagaactac tatatcggtg acctgagcac
    14341 cgcgggcgcg cattgcggta aaggcttcat gtcccggagt atcgagaaag gtaataaatt
    14401 tcttattagg caattcaact ttatacgcgc caatatgctg agtaattcca cccgattcac
    14461 ctgcgactac attcgaacgg cggatataat cgaggagaga ggtcttcccg tgatcaacat
    14521 gtcccattat tacgacaact ggcggacgcg gagtggcatt ttcgaggtct tccttagtat
    14581 cttcaatctt tatttgatct tcgccatagt gttccatttt ttcaacttca aaaccgaatt
    14641 cgtcacatag aaccgagatt gtatcgaaat caagacgttg gtttaccgtt acaaacgttc
    14701 ccagctcgat acatttttgg attacatcgg cggcatctat ccctattaag gaggcgaggg
    14761 aatcaactgt agcaaaatca cttatgcgaa tttttttgct aacctcagat tcttcagcaa
    14821 cagtttcagt ttttttatgt ctttttttcg ttgacttttc atccatttta gccattgttt
    14881 tgcgaatctg agcatctact acgcgatcgt tagcttcccg ctcattacct ggttttttgc
    14941 gacgatggct tacttcatct cctgtttccg tcggtttgtg tttgccatgt ttcagatcca
    15001 gacgagtttc gagttcatca atttttatcc gacgcagttt tttcttacct tttttcttag
    15061 gagattctaa cggaggagtg gtagtagtag ttttttcaat actgggttct gcgatttcag
    15121 tgatttccgg ttcttttggt atttcgggaa gtctatcggt aaccgcggct gtttcccaga
    15181 cagattcaat tagctgactt tgaacgttta gactatattt aaatacatcc ttaatggcac
    15241 tattaaaaaa cagcaaggct gatttataga cttcttcttc gagtttacgc ctctccatct
    15301 cagccgtacg acgcgcgtct tcttccgctt tacgtctatt ctcagcttca cggcgggctc
    15361 gctccaaacg taatctttcg acaatatctt tttctttagc aaattcgccc agaattttca
    15421 tataagaaga ttcgtcaacc ggcgtcatta tgcttttaca gggaattcct tctttctcaa
    15481 gatattttat gatatcatta tgagagatat ttaattcctt cgcaatctcg aaaatccgtt
    15541 tgggtttatc ttttgggtca atactcattt gtttatttct cttcctgacc gaaatcgtcg
    15601 atgatgtctt cgtcggataa gtctgtctct tcctcaacgg tttgtttttc tttattttct
    15661 tgtgcctctt gcagggcggc ttcgcgctga gccttggcgg cttcatggat tgtagcaact
    15721 tcggctttcc gcttttcctg agcttcgatg attatcggtt caagtttatc gagggttttc
    15781 tcgcctaatc ctttgatact caataactct tcacgaggag tgtctaaaaa ttcttcggca
    15841 gtttcgatac cataactata taacaatttc cagttatgct cagtaattcc ttcaatatct
    15901 tccagataaa gggtctcgga ttccgttaat tcgtagtcgg attttttaat agcgtcaatc
    15961 gtatagccgg taacttcgga tgccagtcgt atattttgtc ccattttacc gatagctacg
    16021 gaaatttcat catcggcaaa aatagccaaa gcggttttct tctcctcatc aatttccagt
    16081 tcgagcggtt tagctggtga aagtgcgcgc gtaatcagaa tttcaggttc actactgtaa
    16141 tttataatat ctattttttc attgtttaac tcacgaacaa tcgcctgaat gcgactacct
    16201 ttaataccaa cgcaggctcc cacagcatca attcgtcggt cattggattc aacgataacc
    16261 ttactgcgat caccggcatg gcgagcaact tttttgattt ctatcgtctt atcataaatt
    16321 tccgggactt cattctcgaa cagcttcacc agaaatgatt tgtcggcacg cgaaacaata
    16381 atttccggac cgcttggtcc cattatggct tccttgataa tggctttaat attctcgccg
    16441 cggcgatagc gttcattcgg tatttgttct tcacgcggca tttttagttc ggctttacca
    16501 attaagataa aaatcgcatc gcgtctaatt tgatgaacat cgccaattac gatttgtcca
    16561 atttttttgg cgtattcttc gtaaatctgg tttttctcca ctcggacaat ctgctgatta
    16621 agaatctgtt tagcgtgtga tattaagcgc cgtccaaagc tcatcgggtc tatcacctca
    16681 ataaacggat ctcctacctg cagatctggt tccgtttttc gggcattttc caaagtaatt
    16741 tcgtgcggcg aaccagtggg attatctacg atcgtcatct ggtgataaat ttcaatttca
    16801 cctttatcca tattgacgat gacatcaaaa ttttcggtcg agccgtattt cttttctatt
    16861 aaggtcagaa atactttttc aattatgtca ccgagttcat tccgatcaac gtttttttca
    16921 cgagcgatct gagaaaaagc ttcgatgatt tcacggttta ccaattttgg aagtctccta
    16981 tatgttttac cattgaatta ttactttggc gaaatcaatt tcgcccattt ttagggtatg
    17041 agtaccttca gcattcgcta taacaagttg gtcttcatca acggattcca gttttccttt
    17101 tagcggtgac tttagatttt cactatgatg gaatacgttc atttcgcgcc caagattacg
    17161 atgaaaatca cggaaatttc tcaatgggaa atcaattccc ggcgaactaa cttccagctg
    17221 aaaattatcg ggaaagagtt cattaaatgc tacatcgtct cgcaccgcgc ggcttatttc
    17281 ggcaatctga tcgagggcaa ttccgtgatc agtagtaatt aaaaccttaa caaaagtacg
    17341 atagccctga cgattaactt taaagccaac aacataagca tcgaatcgtt ctacgatctt
    17401 atctacaatt gctcttgccg cgttcttggt agtctctaat tccatgctac acttctttca
    17461 aactaaaagt gggcttttaa acccacttac tatgcggcaa atttaggtaa tttaggctta
    17521 ttattgcaac ttattttagt gccaacccct tgcgagtggt gatttgggct aataaaagct
    17581 cagctgaatt cttattaacg gattcgggtt cgtcgttaag aattatattt acgatttctt
    17641 ccgccttctc aagattgttt acttttaaat aattacgcgc cgaatttagg agtaattcgt
    17701 gcttatgaaa cttgtgtggc gagatttttc ctcctttttc aaaatactcg gcggctaatg
    17761 caaagttgga atcttgttcg gcacaaattg ccaaaccgga ataagctcca gctgttaaaa
    17821 atttgtcatc tttataatca tcaatgtact tttcaaaatt tactcgggca ttttcataat
    17881 catctttagc taaataacag cgtgctagga gaatagttgc catgccagaa ctacgtgtcc
    17941 gagaatactt ttcaatcaaa ttttcgagtc ggagaatggc gtcatcgtaa tcattgcggg
    18001 caaatgacat ttcggccagt cccagttctc ccattgccgc cgtgttggct tttttcttgg
    18061 aatttaccag taaaatagtc agcaagacga cgaccaccac tccgagtaat atgtacgata
    18121 ctaatttgcg ctgtttgcgg accagatttt caatttgtga gattttttct aagaccgggt
    18181 cttttttcaa ttctttccgg gttatcttct ttttaggttt taacatctat ttactcctgt
    18241 tgtaattttt cctgtaccct cgccgggaat tgaacccaga tcaaaggatt aggaatcctc
    18301 tattctatcc atttgaacta cgagggctca aaaagcgggt aaagttatat ttatagacga
    18361 tattttgcaa gctacaataa agtcaacggg gaaataataa tgctgtatct aatcaataat
    18421 agtttctgaa ttcgttataa ataattcggc gccactctct catgatttat ctatttcttg
    18481 gcaaaatatt aatttttttc aggttctggt tcaaaatttc aaccgattcc ggtgaaattg
    18541 aggtttcggt atagatttga gatatcagag ccggattatt tcttaaacgc ttataatccg
    18601 tttctcgtcc agttattata tttcccagtg cttccttaat gcccataccg attaaaattt
    18661 ggctgttacc tttccgaaaa taacacgatg gacgattata tcttttcggg aaaagcgcca
    18721 gaataaaacc gccatattcg gggaaatatt ttagcataat gttcaaacgc ggctcatttt
    18781 ccctcattcc gagcgccggt atcagactgc cgtaatctgg gacgttttta attcctttga
    18841 tgttttggct gacgtccttt aaaagtatta aataatggtc gaaaaattta agtaaagcct
    18901 cagcattttt cgtgactaca atatggaccg gtcgcagaaa gttttcaata taaaatacct
    18961 ccagatcacc agattttaga attcttttga gacgtgcttg accaaccgat tgcccggcta
    19021 atagtttctg gatctggatt tcgccaatta gtttatcttt ataaccggct tgaaaatgga
    19081 agtgggggct cgatgcaccg gctaatattc cattaaagta aatcgaaaaa tgatacagag
    19141 cgcgggaaat ttgaatcata tcattaaatc gccccgttat caactgctta tcatgcttta
    19201 gggcggctat ggttaaatca ccgggataag tgattccggg attggtcagg ataataaatt
    19261 cttgttcatt gccaacttta attattatcc cacgctgtcc ccgatcgagt ttacatagtt
    19321 cacaagtttt tttgattgta tctgtgtttt cgccaaatga actggagacc agactcggcc
    19381 gacgagtttt attcagcatc acatgtactg gaatcgtttt tacgattaat tccgttatcg
    19441 ctacctgact ataaatctgc atatttttaa tgagcttggg ataacctttt tcgggatcgt
    19501 tttgatggag gatgagagct tccgctctat aattgtcgtc aactttttct aacttgatat
    19561 ctaactgctg taaataaacc gccagttctt catcacgtaa aaatctttta gtaacattca
    19621 ttctttaaag accttgttct gttctatgaa ccattcctat tagcctattt tttaaataat
    19681 ctgatggtaa gctacgcatt tgtgatttca gaggcaatta ttagaaagtc ttagtgaaat
    19741 aatcaagaat ttttatgttt actaaactta aaataggtgt taaggaacat tcaagaatta
    19801 cagcggatta cggcgatttc aaattaaatg gtgatttagg ataaaatatc aataactgat
    19861 actcaagctt tccatcataa atagtctttt ggtattagtc aaaatatctt ggcagtattt
    19921 tttggtcgaa atccaaatat tttttgaaaa aaatccggaa gataactttt aacccgcctg
    19981 cattgcccat ttggcgggca agccgatgtt ttttagaaaa gaaaaaataa atacccttgc
    20041 cccgcccttc ccgtgcgtgt gcatttgaaa ttcaactccc aaaattctct ttacattatc
    20101 aaaataattt tgtatcataa ttatagattg aggttattct atag
//
LOCUS       DAOOMU010000035        20110 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2658_length_20110_cov_27.078484, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000035 DAOOMU010000000
VERSION     DAOOMU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 20110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20110
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2658_length_20110_cov_27.078484"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..776)
                     /locus_tag="PLI55_04910"
     CDS             complement(60..776)
                     /locus_tag="PLI55_04910"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HPB00205.1"
                     /translation="MHTRHLFLLIGQSNMAGRGEMDRVLPISDDRIQMFRNNRWQKAV
                     EPLHDDRADLAGVGLGMSFASSILKRNLVQNVGLLPCAVGSTAIEDWLPGTPHYQRAV
                     AVVKTALATDTYENMLGAILWHQGEDDAKDKILAEKYRDRIVSLVWSFRRDLNMPSLP
                     FISGELGDFINAATGYPYGPDISAVLKELESILPDYACVSAEGLASKDDGLHFNAPSL
                     REFGKRYALAYQRLLSYRLN"
     gene            complement(804..1433)
                     /locus_tag="PLI55_04915"
     CDS             complement(804..1433)
                     /locus_tag="PLI55_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HPB00206.1"
                     /translation="MARNEELYLAILKGDRAKVQAILQNAINLNQDVNELLEETMIPA
                     MREVGERFARNEIYVPEMLISARAMQAGLTLIEPILAKSNHKARGKVAIGTVKGDLHD
                     IGKNLVSIMLKGAGYEVLDLGVNCSVEKYAEAVNAGINILLCSALLTTTMMYMKEIVD
                     YFKSNNAVKIVVGGAPVTQSFADMIGAHAYGQNANDAVRIVANLTGAVV"
     gene            complement(1423..2364)
                     /locus_tag="PLI55_04920"
     CDS             complement(1423..2364)
                     /locus_tag="PLI55_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPB00207.1"
                     /translation="MIPIRLVTGFLGSGKTTLLKNLIRQNQSRRLVFLVNEFSPTDID
                     GSLIANLTPDYLSIPGGSIFCRCLAGKFIEYLQEIPQRFTQDGNEIDELVIEASGVAD
                     PRVIFKMLSETGLDKVYYIREVLNIVDPASLGKLFHTLTSIRAQIEAADIILINKVDI
                     ASPEAIAQTESLIRQVNPSAYVYRTTFSQLPKDLPENPQRLQVPEGEFAPCRDPRYAT
                     IILNSGDMNYEQLRQAITPVADDVYRLKGYLFDSHQYLYVDYSPAGWQIEPASSIEQE
                     PQIAIIINGVNQAKVIEHFKLCGLLENNVYFGAKNGT"
     gene            complement(2361..3767)
                     /locus_tag="PLI55_04925"
     CDS             complement(2361..3767)
                     /locus_tag="PLI55_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPB00208.1"
                     /translation="MNKMENNSIQGLQLVRQAFNRQKTTRVPWVPYVGCHGGKLIGQT
                     AENYLKSGKLIAEGLRKANELYRPDGLPVIFDLQLEAEVLGCELLWADDAPPSVITHP
                     LDSLNGKSLSDLPEFSLAKGRYPEVAQAMDIIRQDFAKDLAIYGLICGPFTLALHLMG
                     NEIFLEMYDHPEKVEELLAYCKEIAFQVSDFYLKNGADIIAVVDPMTSQISPEHFQRF
                     ITPALNPIFDYIRANGGLSSLFVCGDVDRNLECMCKTNCDNVSVDEQISLPKLRELAQ
                     KYNKSFGGNLKLTVALLMGSADDCRLEAIRNIDEGGQVGFILAPGCDIPYNVPPQNLQ
                     AVAEMVYDEYQREVARRTLTAREFEVDWIVLPDYANREEVILDIITLDSATCAPCKYM
                     VEAALKATADLSIKTTVREHKITTSEGIGMMKKLGVRSLPTICIDGEVKFSSIIPDQQ
                     TLKAAILDRWKFKTGQDK"
     gene            3861..4784
                     /locus_tag="PLI55_04930"
     CDS             3861..4784
                     /locus_tag="PLI55_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPB00209.1"
                     /translation="MKNIVLSQDKFIPSQSGANITFHRLAWDNSSQPESIIAGSDLRY
                     YSLASDNTALHSHEFAEFFIVISGKVRHIVNGETQDLQAGMLTFIRPNDVHSFEKLND
                     SVCEMVNVAFKLELLLDLSTYLGNDFFLRRYTGPVLPPTFTLSLVETEELALRLIRLI
                     TLQKTDLDQARLKLKMILAEIFTRFFLEWQTLDPSRPQWFDKLCSEMKRKENLIGGLK
                     TMLKLAPCTQQHLCKCFRQYLNTSPTEFINEQRIKLAAKQIIETDEKILAIALEYGFK
                     SLSRFYKIFHKYYGVSPARLRQISRQKVIPL"
     gene            complement(4969..5799)
                     /locus_tag="PLI55_04935"
     CDS             complement(4969..5799)
                     /locus_tag="PLI55_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00210.1"
                     /translation="MIEQILNWMKHVEIGEGDLTTESIALYPLHSSGRLVMSSDTLFS
                     GRAIMEKLLESAGEQLQVAWLCNEGDEIPAGATIFEFNGNGAEILKIRRLLEWLASSL
                     SGLATATREVVKFLGDHNKKLVAGNSVSPIFEEFEKIAFTTGGGEMSFSGISQRIYLT
                     QNHFIYAGKSPDEVITTLVDELGVARKKIKIEVEVNDPIQFEEINKLDCDVIHLVDFN
                     DEQLRTVFEKLNPRHKTVVHLATLADFRPEYDDYFFKYVAVEDLHRTRHYSPARLIFS
                     "
     gene            complement(5804..6382)
                     /locus_tag="PLI55_04940"
     CDS             complement(5804..6382)
                     /locus_tag="PLI55_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HPB00211.1"
                     /translation="MKKNFILGLILLTVPALILAQPEVKGKAEPCQQALKGSHEMHCQ
                     GQYKTPLADLLKLTDQQKVDHKKIMVKYQRLNIPLQADLKLARLDLKEAIDNLDQKKI
                     DESVKKINDIKAKLFKNKIDQRVEFLKTLTDEQRKILKEQPCGMHKVKKIKRLEGRFG
                     WDDMGKLGSEIDELLGLVMDIDVGDMEIEPED"
     gene            complement(6392..6898)
                     /locus_tag="PLI55_04945"
     CDS             complement(6392..6898)
                     /locus_tag="PLI55_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00212.1"
                     /translation="MKSNWLLLISLILLGFTGQLAAQPHSFDESKISMAERRAKMETL
                     RIYKMTEFLELTPEQSEKFFPRLKQYEKNVRKMQHKQMELVREIDQITQNPDTTITES
                     DVKKYLRAMAQIEKDIIAEKEKFIGGLSSILTPSQQLKYMIFDNRFRRRLLKTINPSP
                     NEMKNQQK"
     gene            complement(6918..7520)
                     /locus_tag="PLI55_04950"
     CDS             complement(6918..7520)
                     /locus_tag="PLI55_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HPB00213.1"
                     /translation="MKICKQIKGYLPLFFTGDLDKSAKRLVENHLKDCPACRKFSYEI
                     QHLIDDLQPDPVNIEPNYGAELVVKIQNRLHNERRPKKLKYYLVPVLGSILIFIIIGF
                     NLMRNQSTSSQWLSNDNIIDLYMDWTHSGYFSEMPEDVFDFVNFNENDLITSELRQKI
                     SSVVLSTNSNLLIDDYIESTAFLTDQDFESVIEEIEKSIL"
     gene            complement(7507..8043)
                     /locus_tag="PLI55_04955"
     CDS             complement(7507..8043)
                     /locus_tag="PLI55_04955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB00214.1"
                     /translation="MAKQNVNHPVYDEQAFNNLVRENHQRIYGLFRRMVGSQAEADDL
                     TQETFIKVYKNLPKFRQESSIQTWIYRIAINQGLNYLRRMKIRQTLGLELADSAAEPD
                     EFQSNQANRNLLRRAIAKLPPRQQMVIILRSFQELSFKEIAAVLNITENAAKVNFSHA
                     VKNLREIFLKMGVTYENL"
     gene            complement(8220..8489)
                     /locus_tag="PLI55_04960"
     CDS             complement(8220..8489)
                     /locus_tag="PLI55_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPB00215.1"
                     /translation="MESSKLYVGNLKYSVTDEQLAQLFAQFGEVKSANVIKGKGFGFV
                     EMGSIEEANKAHDALDGKEFEGRTIRIADAHPPRTEKKETHEKQE"
     gene            9027..10841
                     /locus_tag="PLI55_04965"
     CDS             9027..10841
                     /locus_tag="PLI55_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HPB00216.1"
                     /translation="MFKNHPKGLVVAFFTNMGERFGFYTMMAILVLFLQAKYGMNEST
                     AGNIYSWFYFSIYALALLGGILADSTKKYKTVILFGQIIMFAGYFLLAIPGLSLTISL
                     IGLFTIALGNGLFKGNLQAVVGQMYDNEQYSKIRDSAFMIFYMGINIGAFFAPFAATG
                     IRNWWLKVNHFLHDGSLPAICHQYINGTLKDVSELQTLANRVSLSGPVTDLSAFAHDY
                     LEVFSTGYNYAFGIAAVAMIISLLIYVVFNRHLPNKEKVSAEKNSAIALNIQPMTVLI
                     ALLAGAVVAFLVSLVIDIGTGAAIGLFVAFVTWILIVSKKEEKPRVTALLLVFFVVIF
                     FWMSFQQNGLTQTFFARDYTVKTVDRITYMFFTLENILTVIAFVAGLVLVFGRKRSRR
                     TRLIGGIILVVSGVIGYYLFQQGNDQNPISPEIFQSFNPLFIVSLTFVVMGVFDWLNK
                     KGKEPSTPRKIGIGMIVAAIGFSVLLIGSLNLVSPHELKFVDESGVIKYSPVPDYSRV
                     SPYWLIASYLILTIAELLLSPMGLSFVSKVAPERFQGLMQGGWLLATAVGSKLVFVGS
                     TFWGILDLWQLWLIFVVCCLLSAAFIFSIMKRLEKATS"
     gene            10987..14160
                     /locus_tag="PLI55_04970"
     CDS             10987..14160
                     /locus_tag="PLI55_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPB00217.1"
                     /translation="MTQIKYITGPLKSGKTAKVIQETVDVLRSGQVVGGILPSRDHID
                     YIKREILRSQPVLAPGQLFLGTFYAWAERILDECHYNFQTVPSAEEWLNLHDILKQSP
                     VSNYHSFSGYIQILQDIFKDFRESGLTSAELELLAKNSGERELIEWVRLFNKLRQNYR
                     RSSAGPSGESLTIALELLKNKSNDKLGELLVIDGFYEFTPIQDKILQALTPFFTKIIC
                     TSIFDPSHPIYEYCQNRPGWLGSGETVMLSERTISSDPLRFLNSKLFKELIKHDNFTP
                     PNAVSNWKNEWNTPVKIVQCPSRRSEIETAARTIKRWLSEGMNPAKIGITFRGSYDYQ
                     SLIKLIFPNFGIPVAMEVQNLFLSEPAQLITRIVNLNRENFSRPVFIDLLRLPVLQRF
                     YGADLLQELEIYSTEWGLPSAKSDWTARCRQQIEYLQFVLQIDREENDRERRDAQLQI
                     KHLQAIQPILTRLIEELSLPESAEWSEYHYLLIKILERYYPPDQPDEAQKILPIKSIL
                     ERLVRLRDFHTKTSLEELAGILPHFLDTGENEATLSSSGILCANLMDARAWDVDGLIL
                     LGLIDGEFPAIHPENPLFRKDLRSRLNQLANQPIFAEAAFDLAEEKFLLYLLTTRVNQ
                     RLLITYPESDNKGRALPVSPFIDEILACQPANTNQKIVEWEAIPAGQVLPDSINCSSD
                     SDLLQIVLAEKTAREDIEKLVSLIDRKIFDELVINLNCERRRLAGDGIRNGVLPESRF
                     YPEFLAKPLSTTKIQNYVDCPFAYLCAHIWNIEISREPQIGLDALTEGLLIHKVLEQF
                     VQDFLSGEPQSWSDFLEVADDSFLNKLIVQINALFRPKLYFVPDLLWEQVLNNLMMGL
                     RNFVEEERRYCPTGFAPILTEKMYALDDAFLIGNDPTLPVTITGKIDRIDHNASGERF
                     LVIDYKRSSSGIIDIVNGAQSGAQFQIPLYLLILLKHRPNEQIGGAFYYSFKDGKRTR
                     GFLVDVKFERTSELGSSDLNELLNKTQENATAILEQIYRGNFNLLRRNENRCRGNECD
                     FYDLCRIASGQKNSHQQPATHSHQKRT"
     gene            complement(14116..15624)
                     /locus_tag="PLI55_04975"
     CDS             complement(14116..15624)
                     /locus_tag="PLI55_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HPB00218.1"
                     /translation="MPIIIPFSSEWITFACFLGLILGIVGLSEVLRRSSRLTGEQTRR
                     FVHIGVGIFVIFSPLFFKSALQPALLALIFILLNAVALKAGQMKGMHTTERRSYGTVF
                     FPLTFLILIAMYWYSDPVILMLGMMVMTLADPLASIVGMKVKKPRQFTFWKDPKSVQG
                     SLVVFITTFIIITAFLPLFRWLDGTPIPNWQSLIVMALTVGVVATLAEAISSAGSDNL
                     SLPLSAALMIDVMQSLTFQGQIAVMGWVLFSFLLAYTAYRLKALTAGGAAGAMFLGSV
                     VFSIGGINWVIPMATFFVLSSILSKLGKAHKSQLAIMQEKGSNRDLIQVYANGGVALV
                     MAILYFYTEMDLFYLMFLGSLAAATADTWGTEIGTFSKSQPVHILTGKAVPAGTSGGV
                     TVLGTSGVFVGAGILTLSGIFPLEQSLMKIALIIIISGILGALIDSVLGGTIQAQYRC
                     PVCRKTTEKVKHCNNQATTLISGYRWINNDVVNLVCTLSGALLVGVGCWLLM"
     gene            15692..19096
                     /locus_tag="PLI55_04980"
     CDS             15692..19096
                     /locus_tag="PLI55_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPB00219.1"
                     /translation="MLKLVYMTDLTSEREQFIRNITENQFVCAAAGTGKTTVLVRRYL
                     EILLSGAADCDQIIAITFTDKAANEMKERVRSSLLKNEAGLEPAKIASLIERLNTAPI
                     STVHGFCLRILRDNIGELPFDPLFKICDETAEALLRDQFWQKFLERKIAERDPDLTIL
                     INSITLAELSKLLDLIYEKQAECAPILERYKNLNVTSHLADIEKACRDYNFQLLYEYF
                     SDTTISDIINQISTFKSSKSDDVLQRDFCIILTASAEVLGRRQIPVSVRDGSLRKALG
                     GGKKGTAAAWGNELETVREFHSQLAAKFRLIKDEFHTFDPEIEKLHADVMQSLARLAL
                     DFLQEYRAEMRRKALLDFTGIELETENLLARRSPEIVRYIRNFKHLLVDEFQDINPIQ
                     YRIIQLLQELNPDLITFFVGDEKQSIYRFRGAEVEIFNTLREQSDVQPLSINYRSVQA
                     LMNFYNFFFAQFLGSTPPAERYAAHYPLPIAAKDNSISTEPPVELLVIEEDEEFAARP
                     RQESPKDEVLEAQAIANRIWQLHKAEIIRESGIIREAEFGDMTILLRSRTHQSQFTEA
                     LRQAGIPFYVVTGIGFYDQPEIVDLINYLRVLLNWHDEVALVGTLRSPLVGLNDATLT
                     ELAREESLWKGINQYLKNGKEFALEADEAARLKNFYRNYTELNKQITQLSTAEIINQI
                     VELTNFTALLAGLPDGTQKIANVKKLIDLALEWEKSESLSPIDFIRRIRIYRDKEVRE
                     GEANLSAAKGDAVTIMTIHAAKGLDSQIVFLPLLADKINYRSDRFLFHSDIGAAVAIK
                     ADKNQPHPFHYNQLKHLERRRTLAEEKRLLYVAMTRARSYLVCSATVSGKSDEPDKSL
                     WDLSAEIFVRAHEKNLCRIVKLTKTEIEQTALPSGISLAQPVELNDETLRRIRTQIQP
                     LEFKPPIRNLTATALAEWVVAGEKIASTAEPEADQALSPQELGTLIHKAFSWWDFQSL
                     DSFQNMLADLLRPYRLTAPESQKISAELSDWVKQLLDSDNHLRQRLTSARNRRREVEV
                     VGLYGEMILEGKIDLLIENDDNTLTIIDFKSDRIAEKPDEVLLKKYSTQLDFYAFILE
                     RSSGRKVREQALYFIRNGLLITKTITESVLDEAEQNIRKYISELAK"
     gene            complement(19164..19853)
                     /locus_tag="PLI55_04985"
     CDS             complement(19164..19853)
                     /locus_tag="PLI55_04985"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HPB00220.1"
                     /translation="MEDNRNKIELMRPAITITIIAVIMMLIASAISWQKLPNDVRVPV
                     HWNIAGKADRYGSKFQGLMLMPLFTIFLAVCMVVVPLIDPHYRHIKSSMKAYNIILSA
                     IALLMTFLHFLILMATFGQPIDIMKNVCLAIGLLFILIGNYTPKIRSNYFLGIRTPWT
                     LSSELSWHKTHRFAGRLFILLGALIGLGGLTLPGIAVFVILLVGVIGLLLSVTIYSYV
                     IWQKDPEKRKV"
     gene            complement(19834..>20110)
                     /locus_tag="PLI55_04990"
     CDS             complement(19834..>20110)
                     /locus_tag="PLI55_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HPB00221.1"
                     /translation="YRALSDPTRRQILKLLREKDLTAGEIAEHFTMTKPSLSKHFNIL
                     RSAGLIQGEKSGVTITYHLNVSVLEEAIMALMEIFKLKGENNGRQSE"
BASE COUNT         5613 a         4554 c         4249 g         5694 t
ORIGIN      
        1 taataaccgg ctcaatgctt gagtcgcttt aaactgctta agattttaaa taattagttt
       61 taattcagcc gatatgaaag taatcgctga taagccagcg cgtaacgttt gccgaattct
      121 cgtaaagaag gtgcattaaa atgcaagccg tcgtctttgg acgccaatcc ctcagccgag
      181 acgcaggcat aatccggcaa aatgctttcc agttccttta gaactgccga aatatccggt
      241 ccgtaggggt aacctgttgc ggcattgatg aaatcaccca gttcaccgct gatgaaaggt
      301 aagctgggca tatttaaatc acgccggaaa gaccacacga gggaaactat tcggtcgcga
      361 tatttttcag ccaaaatttt atctttggcg tcatcctcgc cctgatgcca aagaatagcg
      421 ccgagcatat tttcgtaagt atccgtggct aatgcggttt ttactactgc aacagctcgc
      481 tgatagtgag gcgtgcccgg caaccaatct tcaatcgccg tagaaccgac tgcacagggc
      541 agaagtccga cattttgcac taaatttctt ttcagaatgg aggaggcgaa actcataccg
      601 agccctacac ccgccaggtc tgcccgatca tcatgcaatg gctcaactgc tttttgccag
      661 cgattattcc taaacatctg gattcggtca tcacttatcg gcagaacgcg atccatttcg
      721 ccccgtcccg ccatgttaga ttgtccaatc aataaaaata agtgtctggt atgcatcagg
      781 ttttttcctt aatagagcga cgttcagacg accgctccgg ttagattcgc tacaattcga
      841 acggcatcat tggcattctg gccgtaggcg tgcgcgccaa tcatatcggc gaaactctgg
      901 gtaaccggcg cacctcccac gacgattttt acggcattat tacttttaaa atagtcaaca
      961 atctccttca tatacatcat agtggtagtc aaaagcgcac tacataaaag aatattgatc
     1021 ccggcgttaa ctgcttcggc gtacttttct acactgcaat tcaccccgag gtcaagcacc
     1081 tcataaccgg cgccttttag cataatgcta accaaatttt taccgatgtc gtgcaggtcg
     1141 ccttttaccg tgccgatggc aaccttaccg cgcgctttgt gattggactt agccagaatc
     1201 ggttcgatta aggtcaaccc ggcttgcatt gcgcgtgctg aaatcagcat ttccggaacg
     1261 taaatttcgt tccgcgcaaa acgttcgccg acttcacgca tagctggaat catagtttct
     1321 tccagcaatt cattgacatc ttgattaaga ttgatggcat tttgcaagat cgcctgcact
     1381 ttggcgcgat cgcctttcaa aattgccaga taaagttctt cattacgtgc cattttttgc
     1441 tccgaaatat acattatttt ctaacaaacc acataattta aaatgttcga tcactttggc
     1501 ttgattaacg ccatttataa tgattgcaat ttgcggttct tgctcaattg aggacgccgg
     1561 ttcaatttgc cagccagctg gcgaataatc cacatataga tattgatgac tgtcaaagag
     1621 ataacctttc aatcggtaaa catcatcagc tacgggggta attgcctgac gtagttgttc
     1681 atagttcata tcgccggaat tcagaattat cgtagcatat cgtgggtcgc ggcatggggc
     1741 gaattcacct tctggcacct gtaaacgctg gggattttcc ggtaaatcct tcggcagttg
     1801 gctgaaagtt gtccgatata cataggcaga aggattgact tgccggatta gactttcagt
     1861 ctgagctatt gcctccggtg aagcaatatc aactttgtta atcaagataa tgtcagccgc
     1921 ctcgatctgc gcccgaatgc tcgtcaaggt gtggaataat ttgcccagac tagctgggtc
     1981 aacgatattt aagacttccc gaatataata aaccttatct agtccggttt cgcttaacat
     2041 tttaaatatc actcgcggat cggctacgcc gctggcttcg attactagct cgtcaatttc
     2101 attgccatcc tgagtaaagc gctgggggat ttcctgtaaa tattcgataa attttcccgc
     2161 taaacaacgg caaaaaatac taccgcccgg gatgctaaga taatcaggtg ttaaattggc
     2221 aatgagtgag ccgtcaatat cagtcggcga aaattcatta accaaaaaaa ccagacgtcg
     2281 cgactgattc tgacggataa gatttttcaa tagagtcgtc ttgccactac ccaaaaatcc
     2341 tgtcactaat ctgattggaa tcatttatct tgcccagttt tgaatttcca gcggtcgaga
     2401 atagcggctt ttagggtttg ctggtcggga atgatggaac tgaatttcac ctcgccgtca
     2461 atacaaatgg ttggtaaact tctcactccc agctttttca tcataccaat tccctcactg
     2521 gtagttattt tgtgctcgcg aactgttgtc tttatactta aatcagcggt ggctttgagc
     2581 gcggcttcga ccatatattt gcagggagca caggtagccg aatccagcgt aataatgtca
     2641 agaatcactt cctcccgatt ggcgtaatcc ggtaaaacta tccaatctac ttcaaattca
     2701 cgagcggtca atgttctgcg agcgacctcc cgctgatatt cgtcatacac catttcagca
     2761 acggcttgta aattctgcgg cggaacatta taaggtatat cacagccggg cgccagaatg
     2821 aaacctacct gcccgccttc atctatatta cgaatggctt ccaggcggca atcgtccgct
     2881 gaacccatca gcaaagcgac cgtcagtttt agattacctc cgaaagattt attatacttc
     2941 tgagcaagct cccttaattt aggaagtgaa atctgctcat caacggaaac attgtcacaa
     3001 tttgttttac acatacattc cagattgcga tccacatctc cgcatacaaa taacgatgaa
     3061 agtcctccat tagcccggat ataatcgaaa atcggattga gtgccggtgt tataaatcgc
     3121 tgaaaatgct ccggtgaaat ctgactggtc attgggtcaa caaccgcaat aatatcggca
     3181 ccgtttttca gataaaaatc actgacttga aaagcaattt ctttacaata agccaataac
     3241 tcctcaactt tttcaggatg atcatacatt tcgagaaaaa tttcattacc catcaggtga
     3301 agcgccagag taaaaggccc acaaatcaaa ccgtaaattg ccaggtcctt ggcaaaatct
     3361 tgacgaataa tgtccatagc ctgggcaacc tctggatagc ggcctttagc cagactaaat
     3421 tctggtaaat ctgataggct tttgccgtta agcgaatcga ggggatgcgt aatgaccgac
     3481 ggcggagcat cgtccgccca aagcagttca catcccagca cctcggcttc caattgcagg
     3541 tcgaaaatta ccggcagtcc atccggacga taaagttcat tggcttttcg caagccttcg
     3601 gcaattagtt tgccggactt aaggtaattt tccgcggtct gaccgatcaa ttttccgcca
     3661 tgacagccaa cgtaaggcac ccagggtacc cgagtggttt tttggcgatt aaaagcctgc
     3721 cgaaccaact gcagtccctg tattgaattg ttttccattt tattcatagg ttaaatttac
     3781 tttatattca catatcataa aatcacatcg tcgaggtaac ttgtctttat tttatcaatt
     3841 tattgctgta tattatcatt atgaaaaata tcgttctgtc gcaagataaa ttcattccca
     3901 gtcagagcgg cgccaatatc actttccatc gtttagcctg ggataatagc agtcaaccag
     3961 aaagcatcat tgccggatcc gatctgcgct actactctct tgccagcgat aatacagcgc
     4021 ttcattccca cgagtttgcc gaatttttca tcgtcatcag cggcaaagtc cggcatattg
     4081 tcaacggtga gacgcaggat ttgcaagctg ggatgttgac tttcattcgg ccaaacgacg
     4141 ttcatagctt cgagaaacta aacgattcgg tctgtgaaat ggtcaacgtc gcatttaaac
     4201 tcgaactgct cctcgatctc agcacttatc taggcaatga tttcttcctg cgacgttaca
     4261 ccggaccggt tttaccaccg acctttaccc tttcactcgt tgaaactgag gaattggctc
     4321 tccgattgat ccgtctcata acgctccaaa aaactgattt agatcaggcg cgtcttaagc
     4381 tcaaaatgat attggctgaa attttcacgc gcttttttct ggaatggcaa acgctcgatc
     4441 ccagccgtcc ccaatggttc gataaattat gttctgaaat gaagcggaaa gaaaacctga
     4501 tcggtgggct gaaaactatg ttaaaactgg ctccttgtac tcaacagcac ctatgcaaat
     4561 gctttcgcca atatctcaac acttcaccga ctgaattcat taatgaacag agaatcaaat
     4621 tagccgccaa acaaataatt gaaaccgatg agaaaatcct tgccattgca ctggaatacg
     4681 gtttcaagag cctaagtcgt ttctataaaa tattccataa atactatggc gtctcaccag
     4741 cgcgcctgcg tcaaattagt cgccagaaag taattcccct ctaaaaaata gcaacttcgc
     4801 caacaggcga gtataataac gattcgatcc ggttcgggta atcgcttttg ctatttgtaa
     4861 aattctcaaa ttattttaga gacttgcccg gggaaataaa atgaaatatt aatggaaatt
     4921 caacactttg aatttcaatc agttagtatg tattttatga tattaaattt atgaaaaaat
     4981 cagcctcgcc ggtgagtaat ggcgggtgcg atgcagatct tctacggcta catatttaaa
     5041 gaaataatca tcatattccg ggcggaagtc agcgagtgtt gcgagatgaa caactgtttt
     5101 gtggcgcggg ttgagttttt cgaacacggt gcgcagttgc tcatcattaa aatctactaa
     5161 atggatcaca tcgcagtcga gtttattgat ttcttcaaac tggatcggat cattgacctc
     5221 gacctcgatt tttatttttt tgcgggcaac acctaattca tcaaccagag tggtaataac
     5281 ttcatcaggg gattttccgg cataaataaa atgattctga gtcaggtaaa tccgctgaga
     5341 aatgccacta aatgacattt cgccaccacc ggtggtgaag gctattttct caaattcttc
     5401 aaaaatcggg gaaacgctgt ttccggcaac taatttttta ttatgatcgc cgaggaattt
     5461 aacgacttcc cgcgttgcgg tcgccagccc ggagagactg ctagccaacc attccaacaa
     5521 acgccgaatt ttaaggattt cagctccgtt gccattaaat tcaaaaatag tagcaccagc
     5581 cggtatttca tcaccctcat tacaaagcca tgcgacttgc agttgttccc cggcgctttc
     5641 taataatttc tccattatag cacgccctga gaataatgtg tcgcttgaca tcactaaccg
     5701 accggatgaa tgtaaaggat agagggcgat actctcagta gtgagatcgc cctctcctat
     5761 ttctacgtgc ttcatccagt tgaggatttg ctcaatcatc taattaatct tcaggctcga
     5821 tttccatatc gccaacatca atgtccatca ccaagcccag taactcgtca atttctgaac
     5881 cgagttttcc catatcatcc caaccgaagc gcccttccaa ccttttgatc ttttttactt
     5941 tgtgcatacc gcagggctgt tctttaagga tttttctctg ctcatcggtc agagttttta
     6001 gaaattcgac tctttgatca attttgtttt tgaacagttt ggctttaatg tcattgattt
     6061 ttttgacact ttcgtcaatt ttcttttgat cgagattatc aatagcctct tttaaatcca
     6121 gacgagctag ctttaaatcg gcttgcaaag ggatgttcaa gcgctgatat ttaaccataa
     6181 tttttttatg gtcaactttc tgctggtcag tcagtttgag tagatcagcc agcggcgttt
     6241 tatattgtcc ctggcaatgc atttcatgcg aacctttcag ggcttgctga cagggttcgg
     6301 ctttaccttt aacttccggt tgagctaaaa tcagagccgg aacagttaac aggatcaaac
     6361 ctaaaataaa gttctttttc atttatacct cctatttttg ttggtttttc atttcattag
     6421 gtgaaggatt aatggttttc agtaaacgtc gtcgaaaacg attatcgaaa atcatatatt
     6481 tcaactgttg gctaggggtt aaaatcgaac tcaacccgcc gatgaatttc tctttttcgg
     6541 ctataatgtc tttctcaatt tgggccatcg cccgtaaata tttcttaaca tcggattcag
     6601 taatagtagt atcgggattt tgagtaattt gatctatttc ccgaaccaat tccatctgtt
     6661 tatgttgcat tttccgaacg tttttttcgt actgtttcaa cctcgggaaa aatttttcgg
     6721 actgctccgg tgtcaattcc aaaaactcgg tcattttata gatacggaga gtttccattt
     6781 ttgcgcgtct ttccgccata ctaattttcg attcgtcgaa ggaatgcggc tgagccgcca
     6841 attgaccagt aaaacccagt agtattaatg atattaacaa taaccaattg cttttcattt
     6901 gatatcctcc cgcaaattta taatatggat ttttcaattt cttctatgac tgattcgaaa
     6961 tcctgatcgg tcaaaaaggc agtggattca atatagtcgt caattagtaa gttactattg
     7021 gtagatagaa ccacgctact tattttctgc cgtaattcgc tggtaatcag atcattctcg
     7081 ttaaagttca caaagtcaaa aacatcttcc ggcatttcgc taaaataccc ggaatgagtc
     7141 caatccatat aaaggtcaat gatgttgtca ttagataacc attgcgagga agtggattga
     7201 ttacgcatca agttaaatcc gataatgata aaaattaata ttgaaccgag gaccggtacc
     7261 agatagtact ttaacttttt aggacgccgt tcattatgga gtcggttttg gatttttact
     7321 actaattcag ctccataatt tggctcaata ttgacaggat caggctgtaa atcatctatc
     7381 aggtgttgta tttcatagct gaatttgcgg caagcaggac aatcttttag gtggttttcg
     7441 acgagtcttt tggctgattt atccaagtcg ccggtaaaaa atagcggcaa ataaccttta
     7501 atttgtttac aaattttcat aagtcactcc cattttgaga aatatctctc gcaggttttt
     7561 aacagcatgt gaaaaattca ccttggcggc gttttcggtt atgttcaaaa cagccgcaat
     7621 ttccttaaat gataattcct gaaaagagcg cagaatgata accatttgct ggcgcggtgg
     7681 cagtttggcg attgcccgac gtaaaagatt acgattggct tgattgcttt ggaattcatc
     7741 gggttcggcg gctgagtcag ccaactcgag ccccagagtt tgcctgattt tcatccgtct
     7801 caggtaattc agcccttgat tgatggcaat ccggtaaatc caggtttgaa tagaagattc
     7861 ctgcctgaat ttgggtaagt ttttataaac ttttatgaaa gtttcctgag tcaaatcatc
     7921 cgcctcggct tgactgccca ccatgcgccg gaatagtcca taaatgcgct ggtgattttc
     7981 acgtaccaaa ttgttaaacg cctgttcatc atagactggg tggttaacgt tttgtttagc
     8041 caaattgtat aattcctttt cacgttgcgt tagacaaaaa acgcaagtaa aaggttaaac
     8101 ataaattttt agataaaaaa agatatttat cgaacgatta tggcgtttga taaaaagatc
     8161 agcttttccg gaaaaatccg gaatctgaac cttattgaaa ttcgataaga agagctaaac
     8221 tattcttgtt tttcgtgcgt ttcttttttt tcagtccgcg gtggatgggc atcagcaata
     8281 cggatagtcc gaccttcgaa ctctttaccg tccaaagcgt cgtgcgcctt attggcttct
     8341 tcaatcgaac ccatttcgac aaaaccgaag cctttgcctt taattacgtt ggcacttttg
     8401 acttccccga attgggcaaa tagctgggct aattgctcgt cggtaacaga atatttcaga
     8461 ttgccaacat acagtttact gctttccatt caaacctcca taataaattc aagtgtcaga
     8521 gataatgtag attaacgtag aggtcgaagt gaattgcact gctaaccccg acaaggttac
     8581 attatttgac acctatgagt atctccctcg cgcaggatga agatctaatt tggtataaat
     8641 agctaattct gaatcaatct ttccaaataa cgcattattt taataacaaa atttctgcaa
     8701 ttcaaagaaa atctgcaatt aactggattt ttattagaaa ataaacaggt aaaattggta
     8761 ttctatctga aaatgatcgc cattttctaa atatcgcaaa cgatattgat tcaactttga
     8821 caaggttttc acttctacta gtaaaacata acccgttttt acaagattat tttctattgg
     8881 catatttttt tcgccattct tgttaaccta aggtgggaaa ttcgctatta aattttcatt
     8941 ttatttttcc gcctttacac ctatatttcc gtcgctatct tatgactaaa tatttaaaat
     9001 atttcatcta agaggaggtt ttcgcaatgt tcaagaatca tccgaaagga ctggttgtcg
     9061 ccttttttac caatatgggt gaaaggttcg gcttctatac gatgatggcg attctggttt
     9121 tattccttca ggctaaatac ggtatgaatg aaagtaccgc tggcaatatc tatagctggt
     9181 tttatttcag catttacgcg ctggcattat tgggcggcat actcgctgat tcgaccaaga
     9241 aatataaaac ggtgattctc ttcggacaaa ttattatgtt tgccggatat tttttactcg
     9301 ctattcccgg gctatcatta acgataagtc tcattgggct ttttactatt gctctgggga
     9361 atggtctttt caaaggaaat ctgcaagcgg tcgtcggtca gatgtacgac aacgagcaat
     9421 actcgaagat cagggattcg gcttttatga ttttctatat ggggattaat atcggggcgt
     9481 tctttgctcc ttttgcggcg actggaatac gcaactggtg gttgaaagtc aaccactttt
     9541 tgcacgacgg cagtttgcct gcgatttgtc atcaatacat caatggaacc ttaaaagatg
     9601 tgagtgaatt gcaaactctt gccaatcggg tgagtttgag tggtccggtg actgaccttt
     9661 ccgcttttgc tcatgattat ctggaagttt tttccacagg atacaattac gcttttggaa
     9721 ttgccgcagt ggctatgatt atttcgttgc tgatttatgt cgtttttaat cggcatttgc
     9781 ccaataaaga aaaggtgtcg gccgaaaaaa attcagcgat agcgttaaat attcagccaa
     9841 tgacggtttt aatcgcctta ttagcgggag ccgtagtggc atttctggta agcctggtaa
     9901 ttgacattgg caccggagcc gccatcgggc tatttgtggc gtttgtgact tggattctaa
     9961 ttgtttcgaa aaaagaagaa aagccgcgag ttactgccct gctcctagtt tttttcgtag
    10021 ttatattttt ctggatgtct tttcagcaaa acggcttgac gcagacattt tttgcccgcg
    10081 attatacggt caaaacagtc gatcggataa cttatatgtt cttcacattg gaaaatatac
    10141 tgacggtaat tgccttcgta gccggactgg ttttggtatt tggccgtaag cgcagtcgcc
    10201 ggactcgtct gataggtgga attattttgg ttgtcagcgg agttatcgga tattatcttt
    10261 tccagcaagg caatgaccag aatccgattt cgccggaaat tttccagtct ttcaacccgc
    10321 tttttattgt atcgttgact ttcgttgtaa tgggtgtttt cgattggctc aataaaaagg
    10381 gaaaagagcc ttccacaccg cgaaaaatcg gtatcggaat gatcgttgcc gcgatcggtt
    10441 tcagtgtact gctaattggc tcgctgaatt tggtttcacc gcacgaattg aaattcgttg
    10501 acgaatccgg tgttattaaa tacagcccgg tgccggatta ttcccgagtc tcgccctact
    10561 ggctgattgc atcttactta atccttacca ttgcggaatt attactgagt ccgatggggc
    10621 tatctttcgt gtccaaagtc gcaccggaac gttttcaggg actgatgcaa ggtggctggc
    10681 tactggcgac ggcggtcggc agtaaacttg ttttcgtcgg aagtactttc tggggcattc
    10741 tcgacctctg gcaactgtgg ttgattttcg tggtttgctg tctgctttca gccgctttta
    10801 tattctctat tatgaagcga ctggagaaag cgaccagcta aacttctatt tgtaaaaatt
    10861 tcagctgcaa cttcggccgc tttgcaacat tcccgcaaaa cggcagtaag ttggcgacaa
    10921 attctggcaa ataatcgcgt tttgaattac attcgttggg gctgaaaagg atttgagatt
    10981 aatcatatga cgcaaattaa atatatcact ggtccgctga aatccggcaa aactgccaag
    11041 gttattcagg aaacggttga tgttctccgc tccggacagg tagtgggtgg gattctacct
    11101 tcacgcgatc acattgatta tataaaaagg gaaattttgc gtagtcagcc ggttttagcg
    11161 cccggtcagc tatttttagg tactttctac gcctgggcag agcggattct cgacgagtgt
    11221 cattacaatt ttcaaactgt tccatctgct gaagaatggc tcaatctcca cgacatttta
    11281 aaacaatcac cagtgagtaa ttatcactca ttttccggct acattcagat tttgcaggac
    11341 attttcaaag attttcgtga aagcggattg acatcggcag aattggaatt attagccaaa
    11401 aattccggcg aacgagagtt gatcgaatgg gtccggttat ttaataaatt acggcaaaac
    11461 tatcgtcgga gtagtgccgg accttcaggc gaatcgctca cgattgccct tgaactactt
    11521 aaaaataagt cgaatgacaa gttaggtgag ttactggtaa ttgatggttt ttacgaattc
    11581 actccaattc aggacaaaat cctgcaagct ttgacgccct tttttactaa aataatctgc
    11641 accagcattt ttgatccctc tcatccgatt tatgaatatt gccagaatcg tcccggctgg
    11701 ttgggttcgg gcgaaacggt catgctttca gaacggacaa tttcgtctga tccgctaaga
    11761 ttcctgaatt caaaactttt caaggaatta ataaaacacg ataatttcac gccaccaaac
    11821 gccgtaagca attggaaaaa tgaatggaac acgccggtaa aaatcgttca gtgtcccagt
    11881 cggaggtcag aaatcgagac tgccgctcgc acgatcaaac gctggttgtc agaaggaatg
    11941 aatcccgcca aaatcggaat tacatttcgg ggtagttacg actaccaaag cttaatcaaa
    12001 ttaattttcc cgaattttgg aattccggtg gcaatggaag tccagaacct attcctcagc
    12061 gaaccggctc aattaataac tcgcatcgtc aatctcaatc gggaaaactt cagtcgcccg
    12121 gtctttattg acctactgcg cctgccggtc ctacaacgtt tttacggcgc ggatttattg
    12181 caggaattgg aaatttattc gaccgaatgg ggtttgccat ctgctaaatc cgactggaca
    12241 gctcgatgtc gccagcagat cgaatatctc cagttcgttc tgcaaattga tcgggaagag
    12301 aacgacagag agcgccgcga cgcccagtta cagatcaagc acttacaggc gattcagccc
    12361 atcctgactc gtttaattga ggaactgagt ttgccggaaa gcgccgagtg gagcgagtat
    12421 cattacctat taattaaaat attggagcgt tattacccgc cagaccagcc ggatgaggcg
    12481 cagaagattc taccgataaa gtcaattttg gaacgactcg tccggctcag ggattttcat
    12541 accaagacca gtctggagga attagccgga attttaccgc attttctcga tactggagaa
    12601 aatgaggcga cgctttcttc gtctggaatt ctttgtgcta atttgatgga tgcgcgggct
    12661 tgggatgtgg acgggctgat tttactggga ctaatagacg gcgagttccc ggctattcat
    12721 cctgagaacc cgttattccg caaggattta agatcgcgac tgaatcagtt agctaatcaa
    12781 ccaattttcg ctgaagccgc ctttgactta gctgaggaaa agtttctatt atacctcctt
    12841 accacgcgcg ttaatcagag gctattgatt acttatccgg aatcggacaa taagggacgt
    12901 gctttaccgg tttcgccatt cattgatgaa atattagcct gccaaccggc gaatacgaac
    12961 caaaaaatcg tagaatggga agcaattcca gctggacagg ttctcccgga ttctattaat
    13021 tgtagttcag acagtgatct tctccaaatt gtgctggctg aaaaaactgc ccgcgaagac
    13081 attgaaaaac tcgttagcct gattgacagg aaaattttcg atgagttagt aattaacctt
    13141 aattgtgaac gtcggcgatt ggcaggcgac ggtattcgga acggcgtttt gccggagtca
    13201 aggttttacc cggaatttct ggcaaagccg ctttcgacca ccaaaatcca gaactatgtt
    13261 gattgcccgt ttgcctattt atgcgctcac atttggaata tcgaaatttc gcgcgaaccc
    13321 cagatcgggc tggatgctct gaccgaagga cttttgattc ataaggttct ggagcagttc
    13381 gtccaggatt ttctgtctgg cgaaccgcaa agttggtcgg attttctcga ggtagccgac
    13441 gacagttttc taaataaatt aattgtccag ataaatgcac ttttccgacc gaagttatat
    13501 ttcgttcccg atttgttatg ggaacaggtt ttgaacaatt tgatgatggg attgcgcaat
    13561 ttcgttgaag aggagcggcg ttactgcccg accggctttg ccccgattct taccgaaaaa
    13621 atgtatgcgc tggatgacgc tttcttgatt ggaaatgacc cgacattacc cgtgacaata
    13681 accggaaaaa ttgaccggat tgaccacaat gcttctggcg aacgcttttt ggtgatcgac
    13741 tacaagcgct ccagcagtgg cattatagat attgtcaatg gtgcgcaaag cggagcccaa
    13801 ttccagattc cactttattt gctgatactt ttgaaacaca ggcctaacga gcaaatcggc
    13861 ggcgcttttt attactcctt taaagatggt aaaaggacgc gcgggtttct ggttgacgtc
    13921 aaatttgaac gtacgtcgga attggggtcg tccgatctga atgaattact aaataaaact
    13981 caagaaaatg ctaccgcgat cctggaacaa atttatcggg gaaatttcaa tctattgagg
    14041 cgaaatgaaa accgctgtcg cgggaatgag tgtgatttct atgatttgtg ccgaattgca
    14101 tccggtcaaa aaaattcaca tcagcaacca gcaacccact cccaccaaaa gcgcacctga
    14161 aagcgtacag actaaattga cgacatcgtt attaatccag cgataacctg agatcagagt
    14221 ggtcgcttga ttattacagt gtttgacttt ttcggtggtt ttccgacaga ccggacagcg
    14281 atactgcgcc tgaatcgtcc cgcccaatac ggaatcaatc agcgctccga gaattccact
    14341 tataatgata atcagggcaa tcttcatcag cgattgttct aaagggaaaa ttccgctcaa
    14401 tgtcaatata ccggcaccaa cgaatacgcc ggaagtgccc aacaccgtca caccaccaga
    14461 ggtaccagcc ggaaccgctt taccggtcaa tatatgaacc ggttggcttt tcgaaaaagt
    14521 gccgatttcg gtgccccagg tatcggcagt cgcggccgct aacgagccta aaaacattaa
    14581 ataaaataaa tccatttcag tataaaaata caagatcgcc ataaccagcg ccaccccgcc
    14641 attggcataa acttggatta agtcgcggtt actacctttt tcttgcataa tggctaattg
    14701 gctcttgtgg gctttgccga gtttggagag aatagacgat aacacgaaaa aagtcgccat
    14761 cgggataacc cagttaatcc cgccgattga aaaaacgacc gagccgagaa acatcgcccc
    14821 ggcggcgccg cctgcggtca aggcttttaa acgataggct gtataagcca gcaaaaatga
    14881 aaacagtacc cagcccatta ccgcaatctg cccctgaaaa gtcaaactct gcatcacatc
    14941 aatcatcaaa gccgcactca atggcaaact caaattatcg gaaccagcac ttgaaatcgc
    15001 ctcagccagc gtagcaacca caccaacggt taacgccatc acgatcaaac tttgccaatt
    15061 gggaatcggc gtgccatcca gccagcgaaa aagcggcaga aacgctgtta tgattataaa
    15121 tgtggtaata aaaacgacta aagaaccctg caccgatttc gggtctttcc agaaagtgaa
    15181 ttggcgtggt tttttgactt tcataccgac tatcgaagcc agcggatccg ccaaagtcat
    15241 taccatcatc ccgagcatta aaatcaccgg gtcagaatac cagtacatcg caatcaaaat
    15301 caaaaaggtc agcgggaaaa aaaccgtacc gtaagaacga cgctcagtcg tgtgcattcc
    15361 cttcatctgt ccggctttca gagcgacggc attcaataaa ataaatatca aagccagtaa
    15421 cgccggttgc aaagccgact tgaaaaataa gggactgaaa atcacaaaaa ttccgacacc
    15481 aatatgaacg aatcggcggg tctgttcgcc ggtcaggcga ctactccgac gcaggacttc
    15541 gcttaagccc acaattccca aaatcagacc cagaaagcag gcgaaagtaa tccattccga
    15601 agaaaacggt atgataatcg gcatttattc ttttctcccc ataaaaacgc tcgaatttta
    15661 tgctttttaa aatttatttc caaaagcttt gatgcttaaa ttagtctata tgaccgactt
    15721 aaccagcgaa cgcgaacagt tcatcagaaa cattacggag aatcaattcg tctgcgccgc
    15781 cgccggaacc ggcaagacca ccgttttggt cagacgctac ctggaaattt tactttcggg
    15841 agccgccgac tgcgaccaaa tcatcgctat tacctttacc gacaaagccg ctaatgaaat
    15901 gaaagagcgc gtgcgttcat ctctcctcaa gaacgaagcc ggtttggaac ctgcgaaaat
    15961 cgccagtttg atcgagcgcc tgaataccgc cccgatttct accgtgcatg gtttttgcct
    16021 ccgtattctg cgggataaca ttggcgaatt gcctttcgac ccactattca agatatgcga
    16081 cgagactgcg gaagcccttc tccgcgatca gttttggcaa aaattcctgg aacgtaaaat
    16141 tgccgagcgc gatcctgacc taaccatcct aatcaattca ataacactcg ccgaattatc
    16201 caaattgctg gacttgatct acgaaaagca ggccgaatgt gcgccgattc tggaacgtta
    16261 caaaaatctc aatgtaacta gtcatcttgc cgatattgaa aaagcctgcc gcgattacaa
    16321 tttccaattg ctatacgaat atttcagcga tacaacaatt agcgatatta taaatcaaat
    16381 ttctaccttt aagtcatcca aatctgatga cgttttgcaa agagacttct gtattatcct
    16441 cactgcttcc gctgaagttc tcggtcggcg acagataccg gtttcagtcc gggacggctc
    16501 tttacgtaaa gcgctcggcg gtgggaaaaa aggcacagcc gcggcttggg gcaatgaatt
    16561 agaaaccgtg cgtgaattcc atagccagtt agccgcaaaa ttcagactaa tcaaagacga
    16621 atttcatact ttcgatccgg aaattgaaaa acttcacgct gatgtaatgc aaagcctggc
    16681 gcgcctggca ttagattttc tgcaagaata tcgcgcggaa atgcgacgca aagctctgct
    16741 ggatttcacc ggcatcgagc tcgaaaccga aaacctactg gcgcggcggt cgccggagat
    16801 tgtgcgttac atcaggaatt tcaaacattt gctggttgat gaattccagg acattaatcc
    16861 gattcaatat cgcatcattc aacttctgca ggaactcaat cccgatttga ttactttttt
    16921 cgtcggtgat gaaaagcaat ccatttaccg ctttcgtggg gcggaagtcg agatattcaa
    16981 cactctgcgc gagcagtcag atgttcagcc cctttcgatt aattatcgga gcgttcaagc
    17041 tctgatgaat ttctacaatt tcttctttgc tcaatttctc ggtagcacac caccggcgga
    17101 acgttatgcc gcgcattacc cgctaccgat tgcggctaag gacaattcta tttctaccga
    17161 accgccggtt gaactgcttg taatcgaaga agatgaagaa ttcgccgcgc gtccgcgcca
    17221 agaaagtccg aaagacgaag tcctagaagc acaagctatc gccaatcgca tttggcaatt
    17281 acataaagct gaaataatca gggagagcgg cattatccgc gaggctgaat tcggcgatat
    17341 gacgattctc ctgcgttcgc gcacccacca atctcagttc acagaggctc tgcgccaggc
    17401 tggaatccct ttttacgtgg tgacgggaat cggattctac gaccagccgg aaatcgtgga
    17461 cttgattaat tatctgcggg tgttattgaa ttggcacgac gaggtcgctt tagtggggac
    17521 attgcggtcg ccgctggtcg gattgaatga cgccactctg actgaattag cccgtgaaga
    17581 aagtctatgg aaaggcatta accaatattt gaaaaacgga aaagagttcg cgctggaagc
    17641 cgatgaagcc gcacgcctga agaatttcta tcgtaattat actgaattaa acaaacagat
    17701 tacgcaacta tccaccgccg aaataattaa tcaaattgtg gagctgacta attttaccgc
    17761 gcttctggcc ggtttaccgg acggcactca gaaaattgcc aacgtaaaaa aattgattga
    17821 cttggcactg gaatgggaaa aatctgaaag cctctcgccg attgatttta tccggcgtat
    17881 tcgcatttat cgagataagg aagtacgcga aggcgaagcc aatctatccg ccgccaaagg
    17941 tgatgcggta acgattatga cgattcacgc ggcaaagggt cttgacagtc aaatcgtctt
    18001 tctgcctctg ttggctgata aaatcaatta ccggagcgat cgcttccttt ttcattctga
    18061 tatcggtgcg gcggtcgcca ttaaagccga taaaaaccaa cctcatccgt tccactataa
    18121 tcaattaaaa catctcgaac gccgccggac tttagccgaa gaaaaacgcc tgctgtacgt
    18181 cgctatgact cgcgcccgtt cgtatttggt ctgctcggca actgtatccg gcaaatccga
    18241 cgaaccggat aaatcgctct gggatttaag tgccgaaatt tttgttaggg cgcatgaaaa
    18301 aaatctgtgc cgcatcgtaa aattaaccaa aaccgaaatc gaacagacgg ctctccccag
    18361 tgggatttca ctcgctcagc cagtcgaatt aaacgatgaa acgttgcgga gaattcgcac
    18421 tcaaattcaa ccgctcgagt tcaaaccgcc gatccggaat ttaactgcta ccgctctggc
    18481 agaatgggta gtcgccggtg agaaaatcgc ctccaccgcc gagccggaag ccgaccaggc
    18541 gctttcacca caggagttag gcactttgat tcacaaagcc ttcagctggt gggattttca
    18601 gtcgctggat tcgtttcaaa atatgctggc tgatttactg cgtccctacc gactaaccgc
    18661 acctgagtcg cagaaaatta gcgctgaact ttccgactgg gttaagcaat tgctcgattc
    18721 tgataatcat ttacgccagc gattgacttc cgccagaaat cggcgacggg aagtcgaggt
    18781 ggtcgggctt tacggagaaa tgatcctcga gggtaaaatt gatctgctca tcgaaaatga
    18841 cgacaatact cttactatta tagacttcaa atccgaccgc attgcagaga aaccggacga
    18901 agttttattg aaaaagtact ccacccagct ggatttttac gctttcattc tcgaacgcag
    18961 ttctgggcgg aaagttagag aacaggctct ttatttcatt cgcaacggtt tattgattac
    19021 taaaacaata accgagtcgg tattagacga agccgaacag aatattcgca aatatatcag
    19081 cgagttggca aagtaagtat ttttaacgac tcaagttacc tccaagaaaa taaaaaggtg
    19141 atcttcttga tgcgttttgc ggtttatacc ttccgcttct cgggatcttt ttgccagatt
    19201 acataggaat aaatcgttac agatagaagc agtccgatta ctccaactag cagaataacg
    19261 aaaaccgcta tgccaggcaa agtaagtccg cctaagccaa tcagcgcccc cagtaaaata
    19321 aagagtcgcc ccgcaaaacg gtgagtcttg tgccaggaca attcactgga taaagtccat
    19381 ggagttcgta tgccgaggaa ataattacta cgaattttag gggtataatt accgattaga
    19441 ataaataata gtccaatagc taaacacacg tttttcataa tgtctatcgg ttgcccgaac
    19501 gtagccatta atatcaagaa atgcagaaat gtcattagca atgcaatggc tgacagaatt
    19561 atgttgtatg ccttcataga tgatttgata tgccgataat gcggatcaat caatggtact
    19621 acaaccatac aaactgccag aaaaatcgtg aaaagcggca tcaacatcag tccttgaaat
    19681 ttactgccat aacgatcggc tttaccggcg atattccaat ggacaggaac gcgaacatcg
    19741 ttaggtaatt tctgccaaga gatagcggag gcaattaaca tcataattac tgcgataatc
    19801 gtaatggtta tggccggtcg cataagctcg attttattcc gattgtcttc cattattttc
    19861 ccctttgagt ttaaatattt ccatcaatgc cataatggct tcttcaagta cactgacatt
    19921 caaatggtaa gtaattgtaa cccccgattt ttcaccttga atcagtccgg ctgaccgtaa
    19981 aatgttaaag tgctttgaca gagagggttt agtcatggta aaatgttcgg cgatttcacc
    20041 agcagtcagg tctttctcgc gcagtaattt cagaatttgt cgccgggttg gatcggatag
    20101 cgcccgatag
//
LOCUS       DAOOMU010000036        20097 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2663_length_20097_cov_21.990021, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000036 DAOOMU010000000
VERSION     DAOOMU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 20097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20097
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2663_length_20097_cov_21.990021"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..801
                     /locus_tag="PLI55_04995"
     CDS             196..801
                     /locus_tag="PLI55_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00222.1"
                     /translation="MRLTKNQQNALRVARLISVILHPLVIAPLTYALIIAHTSEINRT
                     QKLLYLGLVLIAIIFIPLISVLYLKKRGKTTSLDVPERDKRISPFLVCIAGYFIVWIL
                     LKVSGAPRPISLLMWCYGFNSIIATIITKYWKISVHGMALGGPIAALGFVISPTFYWG
                     ILIAPLIVYSRVKLKAHTIAQVITGFLLGFLLTLIQFGIFL"
     gene            804..2039
                     /locus_tag="PLI55_05000"
     CDS             804..2039
                     /locus_tag="PLI55_05000"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015176220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HPB00223.1"
                     /translation="MIDVLAIILGGGKGTRLYPLTKYRAKPAVPFGGKFRIIDVPISN
                     CLNSGINKIYILTQFNTHSLHRHIYSTYSLGPFSNGFVDILAAQQTMENTDWYQGTAD
                     AVRKNLPFINEIKAAYTMILSGDHLYRMDYRLFLEHHIKNKAEITVAVKPVSRQDAGH
                     FGILQTDEKCRIVQFVEKPNSELLDKLKSPGLPDETPFLASMGIYIFATETLHKILEQ
                     NPSDDFGKNIIPASIPTLNVCAYQFDDYWRDIGTIESFFTANLELARFQPPFSLYDEH
                     APIYTHARSLPASRLENCSLTQTLLADGCHIGESKISESVVGIRTIIQKGTTIEKCVI
                     MGADYFEQVQPEGSPQLGIGQNCVIKNAIIDKNARIGNNVQIVNRDQLKEYESEIFSI
                     RDEIVVIPKNTTIPEGMII"
     gene            2052..2870
                     /locus_tag="PLI55_05005"
     CDS             2052..2870
                     /locus_tag="PLI55_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00224.1"
                     /translation="MYKNYDPQQMPLFPDLFEDIYLPPITTLPEFNQEIDNFIKLADF
                     GALIDLTFQGLDKSYSLRLADFAIPAQMIRDDARPESPIFNLFPPEHHRRLQRFRYEI
                     KAFFNNQNSIKTPNGYFLLRSHFQQWENYRGQFYQKIVNYLQNDIGPRRYRNFFETCF
                     KSGLEWLNAQLRPIHPYQVQFTDLQEISRRRKSLQSTRTTLAQLAPEDPEYLLNCITL
                     KTLHIPLTLVQFIKGVAINSTFKTIHLNYLKDVKIETLRDVKHLLESISRQSDQ"
     gene            2909..3538
                     /locus_tag="PLI55_05010"
     CDS             2909..3538
                     /locus_tag="PLI55_05010"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPB00225.1"
                     /translation="MVAEQILARNIRDPELLEAFRQVPRHLFVDDTQRTLAYSDQPLP
                     IGYGQTISQPYVVAFMIAALQIDKSAWVLEIGTGSGYETAILARLAGNVFTIERIGEL
                     SQRAQKVLSQIGIQNVEFRIGNGCLGWPEKAPFSHIIVTAASPEIPPALVDQLALGGR
                     LVIPLGSSCGGQELTLIEKTPLGLNSQKILPVRFVPLIWDSSNTKPFIK"
     gene            3548..4363
                     /locus_tag="PLI55_05015"
     CDS             3548..4363
                     /locus_tag="PLI55_05015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPB00226.1"
                     /translation="MKDWDQNLFEEFVSQIEAVSRGEYNDESFKKFMRIGVPNSLRQL
                     ATILDNLLRRFETRELHLKEAIEELKSARSDLQDLNTLLDSRVRERTQALEEANRRLE
                     ALSTTDALTGIYNRRYFDQELKRETARSIRYNSPLSLLMLDIDLFKIVNDTHGHLFGD
                     EVLKTLGQILLTCLRKNDFCARYGGEEFAVLLPETGEKEAFLVAEKIRTIVAKRKIYF
                     EQAVVSITISIGIAQFNPAGMIDETQLVDAADKALYQAKRSGRNKSVIFDYSV"
     gene            4463..6706
                     /locus_tag="PLI55_05020"
     CDS             4463..6706
                     /locus_tag="PLI55_05020"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPB00227.1"
                     /translation="MRYFPLASVIVLLFQTTLSQNYLWPTNSSRLISSTFGEYRVNHL
                     HAGLDIKTNQRTGYPVFAIENGYIKTIRTSFTGYGKVIYHQLDDGNIAVYAHLDDFAE
                     PLSTIVKAEQERQKRYRIEVNLKQDDLRVTKGQIIGYTGETGTLHPHLHFELRDSLDN
                     PFNPLNTNLTVNDKTAPTISGIAVTPISLDARINGLPMTQTFKTIQKKINHHILKKPI
                     QVEGQFGIEIKVHDTIKGVPNVYPPYGIKLFIDDSLYFQVQYDRFSYEQTHFAIIDRN
                     YQLDHDLGEVYNRLWTIAPHKNMPMNTLPEATGIIDLETGLHSVRIIAYDKNQNQAIV
                     DFKILSAPVTSFESVEIQTGEEGYQLFLSRPAQPIVENFKARWVNKNGHYLRQANLAL
                     TDTTAEFYKLSLIDQPADNEYLKIEAVGFDGNQFQPLFINLNQAEKSSVISLNYTFIH
                     NPKNFLMLLTFSAVPDSNPTFDLQTSNGLGKVELIRISPTEYMTAPVPFAYWRDASAY
                     EIRFDNQLTNIFRGRLNLKCIVPSNENFCLSQDSVFKAVFPRDAVFDSLLVWLETKEA
                     TEIKGGKMVSARYGLHPTNQPLHDSIQVCFKNPVCAQDISQVGIYQYVDKNWQFRGNQ
                     LDVNQDFIWAYSRQTGEFALIKDYTPPAIRNIFPGNGGRFHANDVHYLKATVHDQLSG
                     ITDDQSIVVTLDGQPIIAEYNAPKNQIRYRLSRQLTAGKHALSITVTDRAGNVKTAVS
                     TFTIIPG"
     gene            6715..7407
                     /locus_tag="PLI55_05025"
     CDS             6715..7407
                     /locus_tag="PLI55_05025"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPB00228.1"
                     /translation="MFIPLRDSTMRLTPPYVTVGLITLNCLVYIYQLTLPPEILVAFL
                     NTYGLVSANLFSHPSLRDFLSFFTTLFLHAGFLHLFGNMLYLWIFGDNIESTLGHGRF
                     LLFYILCGVIAGLTQVLITPRSTIPMIGASGAISGVLGAYLLRYPHARVKVLFWFFIF
                     IRQIWIPAGYLLGFWFIVQLSNGLGSLGFQTQGGVAWFAHIGGFLAGIILLLILEPYE
                     RKRMWKKLNQNY"
     gene            7380..7787
                     /gene="cdd"
                     /locus_tag="PLI55_05030"
     CDS             7380..7787
                     /gene="cdd"
                     /locus_tag="PLI55_05030"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPB00229.1"
                     /translation="MEKIKPELLKVAARQAADNAYAPYSHFPVGAALLTNDGKIYTGC
                     NVEASSYGLTVCAERNAIAAAISAGERNFRAMGIFSLTRVSPCGACRQVIWDICGDID
                     IFLFDESEDYQHFRSSELFPIPFDNNQMKGIDK"
     gene            7788..8426
                     /gene="udk"
                     /locus_tag="PLI55_05035"
     CDS             7788..8426
                     /gene="udk"
                     /locus_tag="PLI55_05035"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPB00230.1"
                     /translation="MKKAILIGVAGGTGSGKTSMARNIVHDFDPRDVVIIEQDSYYYD
                     LSDIPLESRGHHNFDHPDAYDFPQLRADMSAILNGQQVKIPIYDYKTHTRSKTESQVV
                     NGQKIIVLEGIMVLYDPELRDMMDIKVYIETAPDIRFIRRLRRDIHQRGRTVDSVIAQ
                     YLETVRPMHEQFVEPTKHFADIIIPEGGQNSVAIDLFKTKVRSLLRHLNEPR"
     gene            8438..9058
                     /locus_tag="PLI55_05040"
     CDS             8438..9058
                     /locus_tag="PLI55_05040"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPB00231.1"
                     /translation="MSQPNLHRVPTDFEEVVAITEKSAGWIEVICGSMFSGKTEELIR
                     RLRRAQIARQRVAIFKPVIDVRYSEDYIVSHNQQKIPSIPIHTAQEILQKAGNAQVLG
                     IDEAQFLDNELVAVCRTLANSGRRVIVAGLDKDYRGEPFEPIPQLLCEAEYITKTLAI
                     CMKCGNPANFTQRIIKSSERVLLGATDIYEARCRACFEPPITEEKK"
     gene            9102..10736
                     /locus_tag="PLI55_05045"
     CDS             9102..10736
                     /locus_tag="PLI55_05045"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HPB00232.1"
                     /translation="MKYIGIIGLLVILGIAFLMSNNRRLISLRVVIWGLLLQLLFAGI
                     ILGKMLISYFTLFIFLLLIIIFIHKDQINTVGHRSKQWLYIGLISIATVLATAAFYWF
                     GKTGWLAELIGLITVAIVIKAIFKVRPWQKELTVAWLTAGLSWLILHGYTGQDIFAFL
                     SQKVEKFLGLATLGAEFLFGNLALDKYFFPNETTWPGFGMQFAFTVLPVIIFFASFMA
                     ILYHLGVMQAIIKAMARFMQWTMGTSGAETLSCTANIFVGQTEAPLLIRPYVDQLTLS
                     ELLTIMVGGFATIAGGVLAGYIKMGVPAGHLIAASVMSAPAALVIAKIIYPETDHPQT
                     AGDVDLPEDTVKSANLLDAAARGVADGLKLAATVGAMLIAFIALIGLVDSILGFLDRL
                     IDGNLLKGTYTAYTNSSGFSPVKGEFSGIFPGSLKSLFSFILRPLAWLMGVSWQYAGE
                     VGNLMGIKISLNEFVAYSALSELIHTGALDQKSIVIATYALCGFANFASIGIQIGGIS
                     SLAPGRRSDLSKVALKAMFGGALASWMTATIAGLLL"
     gene            10793..11437
                     /locus_tag="PLI55_05050"
     CDS             10793..11437
                     /locus_tag="PLI55_05050"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPB00233.1"
                     /translation="MKVILLGPPGVGKGTQAQKLQQERGLRQISTGDILRQAIKNQTE
                     LGKQAEDYIKRGELVPDDLMLGLIAETLFGDDAPQDYILDGFPRTIPQAEGLDRLFAQ
                     HNVDLDAVILLEAEPEAIIKRLSARRTCRQCNAVYNLITNPPKNPLRCDICGGELYQR
                     DDDRIETIQRRLKVYTKQTEPLAEYYRQRGKLKTIAADGTPDEVFQRINRVIGG"
     gene            11444..12031
                     /gene="coaE"
                     /locus_tag="PLI55_05055"
     CDS             11444..12031
                     /gene="coaE"
                     /locus_tag="PLI55_05055"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPB00234.1"
                     /translation="MVTLGVTGGLGSGKSTACKFLAEKGAYVLDADLIAKQLMENDPQ
                     VRDEIIAAFGNDVYKDGKLDTQKLAHRAFASEADQRILNDIVHPRVIDYFQQEVDKMR
                     DKYDLIVLDAPLIFESGFDSRFDHTLLIYTKYKYRLERALRRGNLTREDILRRIELQM
                     PEEDKRELAEFVIENNGTEEQLKSAIEKLYDKLTI"
     gene            12411..14018
                     /locus_tag="PLI55_05060"
     CDS             12411..14018
                     /locus_tag="PLI55_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HPB00235.1"
                     /translation="MKQVIFSWYDGLIFILFFLFVVIFSMYKSRKGKTGEDYFLAGRQ
                     LTWPLIGLSMIAANISTEQFVGMAGQAAGSAGLAIASYEWMAAITMVIVALFLLPNFL
                     RTGIYTFPEFLEYRYSPLARRLMAVYTILIYVFVLVASVIYTGGLTIVTLFSGMKFFG
                     IEVTLIRAIWLIGIITAIYTIWGGLKGIAWADLFFGSSLIFGGFLTLIIGLRHVGGLS
                     SFLEANQDKLHMILPRDNPVLPWTALVIGLWLPNFYYWSVNQFITQRTLAAKNLREGQ
                     LGVIFASFLKLITPFIIVFPGIMAWQLFKDQMTGPGGTDAAFPTIVRNLIPNGFLGFI
                     FAAIGGAVISSLASMLNSASTIFTMDIYQHAVKKELPQQKIVLVGRIATFIFLIIGCL
                     IAPILDNPSLKGIFTYLQEFQGFISPGILAAFAFGLIFKRAPKAAGLVALILNPIIYG
                     LLLIFCGSLPVFASIQIVPIAFLNRMAITFIIIVITMGIITWLKPLDQPVKMPERKDF
                     NMQSSPIVKWLGLTVIGLVIVLYIIFW"
     gene            14050..15099
                     /locus_tag="PLI55_05065"
     CDS             14050..15099
                     /locus_tag="PLI55_05065"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HPB00236.1"
                     /translation="MPVDFSQIPHRRFNPLTGEWILVSPHRTERPWHGSQEPVASEKR
                     PEYDPECYLCPGNRRASGTVNPLYTSTYVFTNDFPALFPDTPHRRRNVRKYIRDESVS
                     GTCRVICFSPRHDLTLAEMAVEEIRDVITVWAEQTKELGENFSWVQIFENKGAMMGCS
                     NPHPHGQIWAVNSLPNEPYKENRRQSAYFKQNKSVLLTDYLDYELESRERVVLENENW
                     LAVVPFWAIWPFEILLLPRRPILRLPDLTPAERNSLALILKSLLTKYDNLFQSSFPYT
                     MGWHGAPTMPGNFDYWQLHAHFYPPLLRSSSVKKFLVGYEMLAEAQRDLTPEVASRTL
                     RETSEIHYKSLIKNK"
     gene            15109..16407
                     /locus_tag="PLI55_05070"
     CDS             15109..16407
                     /locus_tag="PLI55_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018887199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HPB00237.1"
                     /translation="MPKNQITNLPQHQIDTTVFQKIYGHNPSIIAEQIKRYTYLADRH
                     NEIFAASPALFFSAPGRTEISGNHTDHNHGKVLAASVDLDSIAAVNAINGDQITIFSE
                     GYPQPFIVDINHCSPVEAERGTTTALVRGIVARFKELGLRVGGFNATVTSNVLVGSGL
                     SSSASIEVLLGTIMNYLFNDGQISAEDIALIGQFAENQYFGKPCGLMDQLAIAVGGVV
                     AIDFKNPSKPIIQKVNYDPEQQNYSWLIVNTGTSHVNLTEEYAAIPREMRAVARALGG
                     QVCRDITMQNLIEEIPNLRPKVGDRAILRAFHFLTENERVQQQISALEKGDFAAFLEL
                     ANESGTSSCRWLQNCYPTSNPTEQGVPLALALTQYFLKTVGKKGACRIQGGGFAGTIL
                     VFIPNEVLSDYLKLITKVFNEGSVSQLNIRPIGAVCLNKI"
     gene            16632..19319
                     /locus_tag="PLI55_05075"
     CDS             16632..19319
                     /locus_tag="PLI55_05075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPB00238.1"
                     /translation="MLYFKVSYHRLLIFSVGLLIAIICRAMAMLDTDSDVKNLTILSD
                     TNPRLLTDGTLYTDLQLSPPDEDYLELVSTKTINDSVGMTTKSNQLDSIVNNTGNLPP
                     YPPYDPSPTRQQILQPGGYLEWMMGEDPNGDEISFCEIIIRNELDQDQMITVELNDSI
                     LEKAREGSVEGFSYEYNGQVRFGLKRIQDKDFFRDGWVYSWSVRVSDKWGGTAESNWT
                     EATFRFDDGINQPPLPPVEGFNPDGIIVTSHYLELKWSPATDPDVADRLRYEIVLSRD
                     RSFGGRTYILLKSAYDQNQIVVQTPLLENCQYFWRVRSIDLAEERSVWSKSNTFWVNQ
                     INEAPQRPVRIIHPHNLNEITPNSYFWWLPSDDPDPGDQLNYQIECSESLNFTEPIFS
                     YRIPRTITAKEVETKLELPQRAIGVCLDEISAISLLKDNTLYYWRIRAFDQSEISGTS
                     ASRPVRVAFNYQNDPPLPVVKGFYPADDAIIKTLRPEIRWANSSDPDFSDFQASLTYQ
                     LEISRDPQFPENACSVYTTEPGQTVFTLPENLIENERWFYRVRACDQHGSYSLWSPTN
                     SFITNAISEAPYPVTDGFLPKDSAVVETAQPLISWLPSDDPDPDQDERDLYYLVRFYE
                     ADNPKKVSQVASKTGITSLHLPELKEDKYYFYQISAVDPDGNRSEWSRLVCFGVNAVD
                     LPPSPFALLAPFNGQDSVALDAGFVWHIAHDKDPGSQINYTLYYGTDSLFLTNFREIM
                     FEQPEEDTIMVYNPPERLNYATRYFWKVVATDNRGNQCWASQTNQRPFVFTTVGTQRS
                     IASIFKNFRLHQNYPNPFNIETLIRYDVPFYGPVDVSIYDLIGMKVTTLATGNHAAGS
                     YSVYWNGTDQNGTLVANGVYICRLTAPGYMSHIKVVLMR"
     gene            complement(19610..19741)
                     /locus_tag="PLI55_05080"
     CDS             complement(19610..19741)
                     /locus_tag="PLI55_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00239.1"
                     /translation="MTTESENGNPKEREAELIQKIRNRVAEVQELVDEMKSRLTDKK"
BASE COUNT         5477 a         4511 c         4309 g         5800 t
ORIGIN      
        1 attagtcggg cagggtggtt aggacaccac gctttcaata gtaataatca aatagtcaat
       61 taattgagcg cttttacaaa ctttatgttt tttcgcgtga tttatttgtt gaaattgata
      121 taaaaagaac gtataatttt acggaatgaa atataataga ttattgattc ataactatcc
      181 cagactatag tcattatgcg attgactaaa aatcaacaaa atgcacttcg agtagcccgt
      241 ttaatctcgg taattctgca tccactcgtc atagcgccgc tgacctatgc tttgattatt
      301 gcgcatactt ccgaaatcaa tcgaactcaa aaattgctat atttagggtt agtgcttatc
      361 gccatcattt ttatcccact catttctgtc ctatacttaa aaaaaagagg gaagacgacc
      421 agcttagacg ttcctgaaag agataaacgg attagtccct ttttagtctg tatcgcaggt
      481 tattttattg tatggatttt gttaaaagtg tctggggcac cgcgccctat ttctttacta
      541 atgtggtgct atggatttaa ttcaattatt gccactatta tcaccaaata ctggaaaata
      601 agtgtgcatg gtatggcatt gggcggtcct atagcggctt taggctttgt aatttcaccg
      661 actttttact ggggtatttt gatcgctcca ttgatcgtct attcgcgagt gaagttgaag
      721 gctcacacta tcgctcaagt aattacgggt tttctattag gatttttatt aactttaatc
      781 caattcggga tttttttatg aaaatcattg acgttttggc aattattctt ggcgggggca
      841 aaggcacacg gctttatcct ttaaccaaat atcgagccaa gccagctgtt cctttcggtg
      901 gcaaattccg cattattgat gtaccgatta gcaattgcct caatagcgga atcaataaaa
      961 tctacattct tacccaattc aatactcatt cgcttcatcg tcatatttac agcacttata
     1021 gcttaggtcc ctttagtaat ggttttgttg atattttggc ggctcaacag actatggaaa
     1081 acactgactg gtatcaggga actgctgatg ctgttcggaa aaaccttccg ttcatcaatg
     1141 aaatcaaagc cgcctatact atgattcttt ctggtgacca cctctaccgt atggattacc
     1201 gtttgtttct ggaacatcat atcaagaata aggcggaaat tacggtagcg gtcaagccgg
     1261 tttcacgaca agatgccgga catttcggca ttttgcagac cgatgaaaaa tgtcgtatag
     1321 ttcaatttgt ggaaaaaccc aattcggagc ttctggataa gttaaaatct cccggcttac
     1381 cagacgagac gccctttcta gcttcgatgg gcatctatat ttttgccacg gaaacccttc
     1441 ataaaattct cgaacaaaat ccgagcgacg atttcggtaa aaatatcata cccgcttcaa
     1501 ttcctacgct taatgtctgt gcctatcaat ttgatgacta ttggcgggat attggtacca
     1561 ttgaatcgtt ttttaccgcc aatcttgaac ttgctagatt ccaaccgcct ttttctttat
     1621 acgatgaaca cgctcctatt tatactcatg cccgctcctt accggcttcc cgattagaga
     1681 attgttcact gactcaaact ctgctggcag acggctgtca tatcggtgag agcaagatta
     1741 gcgaatctgt tgtcggtatt agaacgatta tccagaaagg taccaccatt gagaaatgcg
     1801 tcattatggg agctgattac ttcgaacagg ttcagccaga aggttcacct caattaggaa
     1861 ttggccaaaa ttgtgtaatt aaaaatgcta taattgacaa gaatgcccgc atcggtaaca
     1921 atgtccagat tgtaaatcga gatcaactaa aagaatatga gagtgagatc ttctcgatcc
     1981 gggatgagat tgtcgttatt cctaaaaaca ctacaatccc ggagggaatg ataatttaac
     2041 aggaatccgt aatgtataag aattacgatc cccaacagat gcctcttttc cccgatttat
     2101 tcgaagacat ttatctgccg ccgattacca ccctgcccga attcaaccag gaaattgaca
     2161 acttcattaa actagccgat tttggcgctt tgattgattt gacttttcaa ggattggaca
     2221 aatcatattc gctccgactc gctgacttcg cgattccagc tcaaatgatt cgcgatgatg
     2281 ctcgtccaga atcgccaatc tttaaccttt tccctcccga acatcatcgt cgacttcagc
     2341 gctttcgcta tgaaattaaa gcctttttca ataatcaaaa cagtatcaag acgcccaacg
     2401 gctacttttt acttcgcagt cattttcaac agtgggaaaa ttatcgaggt caattttatc
     2461 aaaagatagt gaattacctt cagaatgata ttgggccgcg ccgttaccgc aatttttttg
     2521 aaacctgctt caaatccggg ctggagtggt tgaatgctca actgcgtccg attcatcctt
     2581 accaggtcca attcacagac ctacaggaga tcagccgacg gcgtaaatca ttacagagca
     2641 ccagaactac tttagcgcag ttggcccctg aagatcctga ataccttcta aattgcatca
     2701 ccttgaaaac tttgcatatt ccgctgacgc tcgttcaatt tatcaaagga gttgccatca
     2761 actcgacttt taagacaatt catctaaatt atctgaaaga cgtcaaaatc gagactctac
     2821 gcgacgtaaa acatctcctg gaatctattt cgcgtcaatc ggaccaatga acaatttttc
     2881 accagataat tgccagacag ccgacaaaat ggtcgccgaa caaattttag cccgtaatat
     2941 tagagacccg gaattattgg aggcttttcg acaggtaccg cgtcacctct ttgttgatga
     3001 cactcaacga acgctggcct atagtgacca gcccctgcca attggatacg gtcagactat
     3061 ttctcagcct tatgtggtag cctttatgat tgccgctttg caaattgata aatcagcctg
     3121 ggtactggaa atcggcaccg gtagtggcta cgaaacagca attctagccc gattagccgg
     3181 caatgtattc accattgagc gcatcggtga actttctcag cgcgctcaaa aagtactgtc
     3241 ccagatcggc atccaaaacg tagaattccg aatcggaaat ggctgtcttg gctggcccga
     3301 gaaagctcca tttagtcata taatcgtcac agccgccagt cccgaaatac caccggcatt
     3361 agttgaccag ctggcattgg gcggaaggct ggtgattcct ctcggaagtt cctgcggcgg
     3421 tcaggaattg actttaatcg aaaaaacacc gctgggattg aacagccaga agattctgcc
     3481 ggtcagattt gttccgttga tatgggattc cagtaacaca aaaccattca taaaataact
     3541 gtcgaaaatg aaagattggg accagaattt gttcgaagaa ttcgtcagcc agattgaggc
     3601 agtctcacgt ggcgaatata acgatgaatc ctttaagaaa tttatgcgaa tcggcgtacc
     3661 gaattccttg cgccaattgg caacgatact cgacaatcta cttcggaggt ttgagactcg
     3721 cgagttacac ctaaaagagg ctatcgaaga gcttaaatcg gcccgctcgg atctgcagga
     3781 tttaaatact ctgctggata gccgcgttcg ggaacgcact caagccttag aagaagccaa
     3841 tcggcggcta gaagcgctct cgactaccga tgctctaacc ggtatttata accgccgcta
     3901 tttcgaccag gaactaaaac gagaaactgc tcgcagtatc cgctacaatt caccgttgag
     3961 cctactgatg cttgatattg atctcttcaa aattgtcaat gacactcacg gacatctctt
     4021 cggtgatgag gttttaaaaa cattgggtca gattttgttg acctgtttac gcaaaaatga
     4081 cttttgtgcc cgttatggcg gagaggaatt tgccgtccta ttgccggaga cgggagaaaa
     4141 ggaagctttc ttagtagctg aaaaaatccg tacgatcgtc gccaaaagga aaatttattt
     4201 tgaacaggct gttgtcagta ttacaatttc catcggtatc gctcagttta atcccgctgg
     4261 gatgattgat gaaacccaat tggttgatgc cgccgataaa gccctttatc aagccaaacg
     4321 gagtgggcgt aataaatcgg taatctttga ttattccgtt tgagcctaat tgaaatgcta
     4381 ccgattgctt actaaataaa atttgaaaat tggagctaat ttttcttata ttccaccccg
     4441 aaatgaaaat tagttttacg gcatgagata tttcccactc gcctcggtta ttgttctcct
     4501 ttttcaaacc acactttctc agaattacct ctggccaact aactccagcc ggcttattag
     4561 ttcgactttc ggcgaatatc gtgtcaatca tctacacgcc ggcctcgata ttaaaaccaa
     4621 ccaacgcacc ggctatccgg tttttgccat cgagaacggt tatatcaaaa ctattcgcac
     4681 ctcgtttacg ggctatggca aagtaattta ccaccaactg gatgacggca acatcgcggt
     4741 ttacgctcat ctggacgatt tcgcagagcc actctcaact attgtaaagg ctgaacagga
     4801 gcgtcagaag cgttaccgca ttgaagttaa tcttaagcaa gatgatttgc gcgttaccaa
     4861 gggacaaatc atcggatata ccggcgaaac tgggactctt catccgcacc tgcattttga
     4921 actccgcgat agcctggata atccttttaa tcctctgaac actaatctta ctgtgaacga
     4981 taaaactgcc ccaaccatca gcggcatcgc cgttacgccc ataagtctcg atgctcgtat
     5041 taacggattg ccgatgactc aaacctttaa aactattcaa aagaaaatta atcaccacat
     5101 tcttaaaaag ccgattcaag ttgaaggaca attcggaata gagattaaag ttcacgatac
     5161 gatcaagggt gtcccaaacg tttacccgcc ttacggtatt aaactattta ttgacgacag
     5221 cctctatttc caggttcagt atgaccgatt cagctacgag caaactcact ttgccatcat
     5281 agaccgcaat taccagctgg atcatgacct gggtgaagtg tacaatcgtt tatggacaat
     5341 tgcaccgcat aaaaatatgc caatgaatac cctgccggag gctaccggta ttattgatct
     5401 ggagaccgga ctacattcag tgcgcataat tgcttatgat aaaaatcaga atcaggctat
     5461 agtggacttc aaaatccttt ccgctcctgt tacttccttt gaatcggtag aaattcaaac
     5521 cggcgaagaa ggttatcaac tgtttctatc gcgtcccgcc cagccgattg tagaaaattt
     5581 taaagctcgc tgggttaata aaaacggcca ttatcttcgc caggctaatc ttgcattaac
     5641 tgatacgact gcggaatttt ataagttatc attgattgat caaccagcgg ataatgaata
     5701 tcttaaaatc gaggcggttg gctttgacgg caatcaattt caaccattat ttattaactt
     5761 gaatcaagcc gaaaaatcct cggtgatttc attgaattat acattcatcc acaatcccaa
     5821 aaatttcttg atgctcctga ctttcagcgc cgttcccgat agcaatccga ctttcgatct
     5881 tcagaccagt aacgggctag ggaaagtcga actaatccgg atttccccga ccgaatatat
     5941 gactgcgcca gtgccctttg cctattggcg tgacgcctct gcctatgaaa tccgctttga
     6001 caatcaatta actaatatct ttcgtgggcg attaaatctt aaatgcatag tacccagcaa
     6061 tgaaaatttc tgccttagcc aagactcggt cttcaaagca gtttttccca gagatgcggt
     6121 tttcgattca ctgcttgtct ggctggaaac taaagaggct accgagatca aaggcggcaa
     6181 aatggtatcg gcgcgttatg gacttcatcc cactaaccag cctttgcacg attcaattca
     6241 ggtatgcttt aaaaatcctg tatgtgctca ggacatcagt caagttggaa tttatcagta
     6301 cgttgataaa aactggcagt ttcggggaaa ccaactcgat gtcaaccagg attttatctg
     6361 ggcttatagc cgacagaccg gtgaatttgc tcttataaaa gactatacgc cgccagctat
     6421 tcgcaacatt ttcccgggca atggtggccg gtttcacgcc aatgatgtgc attatctcaa
     6481 ggctactgtt cacgatcaac tctcaggcat tactgatgat caatccatcg ttgttacgct
     6541 tgatgggcaa ccaataatcg ccgagtataa tgccccaaag aatcagatta gatatcgcct
     6601 gagccgacaa cttacggccg gcaaacacgc cttatccatt accgttaccg accgggctgg
     6661 caatgtaaaa actgcagtga gtacttttac gataatacca ggttaagaga aataatgttc
     6721 atcccacttc gcgattctac tatgcgcctg actcctcctt acgttactgt cggacttatt
     6781 actttgaatt gcctcgtata tatttaccag cttactttgc caccggagat tttagttgca
     6841 ttcctcaata cgtatggatt ggtatctgcc aatctattta gtcaccccag cctgagagat
     6901 tttttatcat ttttcacaac gcttttctta catgctggct ttctccatct ttttggtaat
     6961 atgctctacc tatggatttt tggtgacaat atagagtcaa ccctgggaca cggtaggttt
     7021 ttgttatttt atatactctg cggagtaatt gccggtttaa ctcaggtgtt aattactcct
     7081 agatctacga taccgatgat aggtgccagt ggtgctattt ccggagtttt gggagcttat
     7141 ctcctgcgtt atccgcacgc tcgggtaaag gttctcttct ggttttttat attcattcgt
     7201 cagatctgga ttcctgctgg ttatttgctg ggattctggt ttattgtcca gctcagcaat
     7261 ggtttaggct cattaggatt tcaaacgcag ggtggagtgg cgtggttcgc ccatattggt
     7321 ggttttttag ccggaataat tttattgcta attctggaac cctatgaaag gaaaagaatg
     7381 tggaaaaaat taaaccagaa ttactgaaag tcgcggctag gcaagccgcc gataacgctt
     7441 atgctcccta ctcgcacttc ccggtcggcg cggcactttt aactaatgac ggcaagattt
     7501 acaccggttg taatgtagaa gccagctcgt acggcttgac agtatgcgcc gaacgcaatg
     7561 cgattgcggc ggcaatttca gccggtgaaa gaaattttcg tgctatggga attttttcgc
     7621 taacccgggt atcaccctgc ggtgcctgtc ggcaggtaat ctgggatatt tgcggcgaca
     7681 ttgatatttt tctttttgat gaatcggagg actatcagca tttccgcagt tccgagctgt
     7741 tcccgatacc attcgataac aaccagatga agggaattga taaataaatg aaaaaagcaa
     7801 tattgatcgg agtggcggga ggtaccggct caggcaagac ttcgatggcg cgaaatatag
     7861 tacatgattt cgatcctcgc gatgtcgtca ttatcgagca ggattcctac tattatgatc
     7921 tcagtgatat tccgttggaa tcgcgtggtc atcacaattt tgaccacccg gacgcctacg
     7981 atttcccgca gttgcgggct gatatgagtg cgatattaaa cggtcaacag gtcaaaatcc
     8041 cgatttatga ctataaaact catactcgca gtaaaactga atctcaggtt gtaaatggtc
     8101 agaaaattat cgttctggaa ggaattatgg ttttgtatga tccggaattg cgcgatatga
     8161 tggacatcaa agtctatatc gaaaccgcgc ctgatattcg tttcattaga cggctgaggc
     8221 gtgacattca tcaacgcgga cgaacggttg attcagtaat cgcccaatat cttgaaaccg
     8281 tgcgcccgat gcacgaacaa tttgtcgagc cgactaaaca tttcgccgat attatcattc
     8341 cggaaggtgg tcagaactcg gtagccattg atctttttaa aactaaagta agaagccttt
     8401 taaggcacct aaacgaaccg cgataaaaag gagcagaatg agccagccta atctacaccg
     8461 cgtccccaca gacttcgaag aagttgtcgc cattaccgaa aaatcagctg gttggatcga
     8521 ggtcatctgc gggagcatgt tttcgggtaa aactgaagaa cttatccgtc gtctgcgccg
     8581 cgcgcaaatc gcccgccagc gagtggcaat cttcaagccg gtaattgatg tgcgctatag
     8641 cgaagattat attgtctctc ataaccagca gaaaattccc tccatcccga tccatactgc
     8701 tcaggaaatt ctccaaaaag ccggcaatgc ccaagtgctt ggaattgacg aagcccaatt
     8761 tttagataat gaattagtcg cggtctgccg aacactcgcc aactccggtc gacgcgtgat
     8821 cgttgctggt ctcgacaaag attaccgtgg cgaaccgttc gagccgattc cgcaactgct
     8881 ctgcgaagcc gagtatatta ccaagacgct ggcaatctgt atgaaatgcg gcaacccagc
     8941 caatttcacc cagcgcatca tcaagtccag cgagcgggtt ttgcttggcg caaccgatat
     9001 ttacgaagcc cggtgtcgag catgctttga accacctatt acggaagaga aaaaatgaat
     9061 tctctcggtt taacaactat attggctaat taggtgtaaa tatgaaatat ataggcataa
     9121 ttggactgct ggtcattctg ggaattgcat ttctgatgtc gaataaccga cggctaattt
     9181 ccttgcgagt cgttatctgg ggactcttac tccaactgct ttttgccgga ataattctgg
     9241 ggaaaatgct gatttcctac tttaccctgt ttatctttct cctattaata ataatattca
     9301 ttcataaaga ccaaattaat acggtcggtc accggtccaa acaatggctt tatatcggtt
     9361 tgataagtat agcgaccgtt ttagctacag ctgcttttta ctggtttggg aaaaccggct
     9421 ggttggcaga gttaatcggc ttaattaccg tagcgatcgt tatcaaggct atttttaaag
     9481 ttcgtccttg gcaaaaggaa ttaactgtcg cctggcttac ggctggatta agttggctga
     9541 ttttacacgg ctatactggg caggatattt ttgctttcct ttcacaaaaa gtcgaaaaat
     9601 ttctggggtt ggcgactctc ggcgcagagt ttttattcgg caacctggcg ctcgataaat
     9661 attttttccc gaatgaaact acttggcctg gattcggaat gcaatttgcc tttacggtgc
     9721 taccggtcat aattttcttc gcttcgttta tggcaattct ctatcatttg ggcgttatgc
     9781 aagccattat caaggcgatg gcgcgtttta tgcaatggac gatgggcacc agcggcgccg
     9841 aaacactgtc ctgcactgcc aatatcttcg tcggtcagac cgaagccccg ttattaatcc
     9901 gcccctatgt tgatcagtta accctttcag aactactgac tattatggtt ggcggatttg
     9961 ccacgattgc tggaggcgta ttggctggat atatcaaaat gggggtaccg gctggtcatt
    10021 taattgcggc cagcgttatg tcggctcccg ccgctttggt cattgcgaaa atcatctacc
    10081 ctgagactga ccatccccaa accgccggcg acgtagattt accggaagat accgtcaaat
    10141 cggccaatct tttggatgcg gcggcgcgtg gggtagctga cggactaaaa ttagcggcaa
    10201 cagtcggtgc aatgctgatt gcctttattg ccctgattgg tttggttgat tccattttag
    10261 gatttcttga tcgtctgata gatggtaatt tactgaaagg cacttacaca gcttacacca
    10321 actcttccgg attctccccg gtcaaaggtg agttcagcgg cattttccca ggcagtctga
    10381 agtcattatt ttcatttatt ctgcgtccgc tggcttggtt aatgggcgtt tcctggcaat
    10441 acgccggtga ggttggtaac ttgatgggca taaaaatttc cctgaacgaa ttcgtagcct
    10501 attctgctct ttccgaattg attcacacgg gtgctctcga tcagaaatcc attgtgattg
    10561 cgacttatgc cttgtgtggt ttcgccaact tcgcttccat tggcattcaa atcggtggca
    10621 ttagctcttt agcacctggc cgacgttcgg atttatccaa agttgctctg aaagccatgt
    10681 tcggcggggc tttggcatcc tggatgacgg ctacaattgc tggattgtta ttatagttga
    10741 tacattagga aaaatatttt aaaataaatc aaaattggat agaggaaatc aaatgaaagt
    10801 aatcttacta gggcctcccg gagtcggtaa gggaactcag gctcagaaac ttcagcagga
    10861 acgtggtctg cgtcagattt caactggcga tattttgcgc caagctatta aaaatcaaac
    10921 ggagctgggc aaacaagccg aagattatat aaagcgcggc gaactggttc ccgacgattt
    10981 gatgctcggc ttaattgccg aaacgctttt cggggatgat gctccgcagg actatatctt
    11041 ggacggattt ccccgtacta ttccccaggc ggaaggctta gaccggcttt tcgcgcaaca
    11101 taacgttgat ctcgacgctg taattttgtt ggaagccgag cccgaagcta ttatcaaacg
    11161 gctttcagct cgacgtacct gccgccaatg caacgcggtc tataatctca ttaccaatcc
    11221 gcctaaaaat cccctgcgat gcgatatctg cggtggtgaa ttatatcaac gagatgatga
    11281 ccgaattgaa accatccagc gacgtctaaa agtctatact aaacaaaccg aaccgctggc
    11341 agaatactac cgccagcgtg gaaaacttaa aacaatcgcg gctgacggta cgcccgatga
    11401 agtattccaa agaataaatc gtgtaatagg aggataaaaa aacgtggtta ccttaggggt
    11461 taccggtgga ctcggctccg gaaaatcaac cgcctgtaaa ttcctggctg agaaaggcgc
    11521 ttatgttctg gacgccgatt taattgccaa acaattgatg gaaaacgatc cccaagtccg
    11581 tgacgaaatc atcgcagctt ttggcaacga tgtttataaa gacggcaagt tagacaccca
    11641 aaaattagct catcgcgcct ttgccagcga agccgatcaa cgcattctaa atgacattgt
    11701 acacccgcgt gtaatagatt attttcagca agaagtggat aagatgcgcg ataagtacga
    11761 cttaattgta ttggatgcac cccttatttt cgagtccggt ttcgacagtc ggttcgatca
    11821 cactttgcta atctacacca aatacaagta tcgtttggaa cgggctttac gtcgcggtaa
    11881 ccttacccgc gaagatatcc tgcggcggat tgaactgcag atgccggaag aagacaaacg
    11941 cgaactcgcc gaattcgtca tcgaaaacaa cggcaccgaa gaacaactaa aaagcgccat
    12001 cgaaaaactc tacgataagc taaccatctg aaccgatttg aatggtggta agtagtatat
    12061 tttacagttt atttctaatc gctatttata catttttcga tattgaaact tttggggaaa
    12121 aaaccttttt ggggtagtga cagtattagc taaattgttt aatcgaaata aatgagagaa
    12181 tgaataacca attaaataga ttaaatagag gtaattttca actcaatcag aactacagaa
    12241 cggtaactaa tgaagttaat ttgcccgagt cggcaaggtg tgacaggaaa aaagattatt
    12301 gaattatcac aaaaataata ggcaaagccg atggcagctg gacaaagggg attttgtgac
    12361 ggcttcgaaa aagcacaatt aaaatttgat aaaaaggggg ataagtcatt atgaaacaag
    12421 taatttttag ctggtatgac ggcttaatat ttatactgtt tttcttgttc gtggtgatat
    12481 tcagtatgta caaaagccgt aagggaaaaa ctggggagga ttactttctg gctggcagac
    12541 aattgacttg gccattgatt ggcctttcaa tgattgcggc taatatttcc accgagcaat
    12601 ttgtcgggat ggctgggcag gcggctggtt cagcaggttt ggcgatcgct tcttatgaat
    12661 ggatggcggc tataacgatg gtgattgttg ccctatttct cctgcctaat tttttacgta
    12721 ccggaattta tacttttccg gaatttctgg agtatcgcta ttctccacta gcgcgccgtc
    12781 taatggcagt ttacactatt ctaatctacg tttttgtgct ggtagcatcg gttatttata
    12841 ccggcggttt gaccatcgtt actttatttt ccggaatgaa atttttcggg atcgaggtga
    12901 ctttgattcg agcaatctgg ttaatcggca taattaccgc catttatact atttggggcg
    12961 gtttaaaagg aattgcctgg gcggatctct tctttggatc gagtttgatt tttggcggat
    13021 ttctaaccct gataatcgga ctgagacatg tcggagggct tagtagcttt ttagaggcaa
    13081 atcaggataa attgcatatg attctgccgc gagataatcc cgtacttccc tggacggctc
    13141 tggttatcgg cctgtggtta ccaaatttct actactggag cgtcaatcaa tttattacgc
    13201 aacgtactct tgccgctaaa aacctccgtg aagggcaact gggagtgata ttcgcctcct
    13261 ttttgaagtt aattacgcca ttcatcatcg tttttcccgg aattatggct tggcagttat
    13321 ttaaagatca aatgaccggc ccgggcggca ccgatgcggc gtttccgaca attgtcagga
    13381 atttgattcc caacggattt ctgggcttta tttttgccgc tatcggagga gctgtaatca
    13441 gttccctggc atcaatgctg aattcggctt ccactatttt cacgatggat atttaccagc
    13501 atgccgttaa aaaggaactc cctcagcaaa agatcgtcct ggtgggacgg attgccactt
    13561 tcattttttt aattatcggt tgtttgattg cacctatcct tgataatccg agcctgaaag
    13621 gaattttcac ctaccttcag gaatttcagg gattcatttc gccaggaatt ctagccgcct
    13681 tcgcttttgg gctaattttt aagcgagcac ccaaagccgc cggcttagtc gcactaattc
    13741 ttaatcccat catctatggt ttactgctga ttttctgcgg atctctgccg gtctttgcta
    13801 gtatccaaat tgtaccgatt gccttcctta accgcatggc aattactttt attattatcg
    13861 ttattacaat gggaattatt acctggttaa aaccgttgga ccagccggta aagatgccag
    13921 aacgcaagga cttcaatatg caatcttcac cgatcgtaaa atggctcggg ttgacagtaa
    13981 ttggactggt catagtgctt tacatcattt tctggtaaat tatcttaaaa actgagaatt
    14041 aattgttaaa tgccagttga cttttcccaa attccgcata ggcgcttcaa tccgctcacc
    14101 ggcgaatgga ttttagtttc accccatcgt acggaacgcc cgtggcacgg cagtcaggaa
    14161 ccagtagctt cagaaaaaag gccagagtat gatccggaat gttacttgtg tcccggcaat
    14221 cgccgagcta gcggaaccgt aaatccactt tatacctcga cctatgtctt cactaacgac
    14281 tttccggctt tgtttcccga tacacctcat cgtcgtagaa atgttaggaa gtatatcagg
    14341 gatgagagtg tatccggcac ttgccgagta atttgcttct cgccgcggca cgatcttact
    14401 ttggcggaaa tggcggttga ggaaatcagg gatgtaatta ccgtttgggc tgaacaaaca
    14461 aaggaactgg gtgaaaattt ttcctgggtc caaatatttg aaaacaaagg tgctatgatg
    14521 ggttgttcca atccccatcc gcatggccaa atttgggcgg tcaatagcct tcccaacgaa
    14581 ccttataaag aaaatcgccg acaatcagcc tactttaagc agaataaatc agtgctatta
    14641 actgattatt tggactatga attagaatct cgggagcggg tggttctcga gaatgaaaac
    14701 tggttggcag tagtaccatt ttgggctatc tggccgtttg aaattctcct cctgccgcgg
    14761 cggcctattt tgcgtttgcc agacctcact ccagccgaga gaaatagtct cgccttaatt
    14821 ctcaaatctc ttctgactaa atatgataat ctttttcaat catcttttcc ctatacgatg
    14881 ggctggcacg gcgccccgac aatgcccggt aacttcgact actggcaatt gcatgctcac
    14941 ttttacccgc cacttttacg ttcatcatcg gtgaaaaagt ttctggtagg ctatgaaatg
    15001 ctggcagaag ctcagcgcga tctgacaccg gaagtagctt cccgaacact cagagaaaca
    15061 agtgaaattc actacaaatc actaattaag aacaaataag gttaaataat gcccaaaaat
    15121 cagataacca acttaccgca acaccaaata gataccaccg tatttcaaaa aatctacggc
    15181 cataatccaa gcataattgc tgagcaaatc aaacgttata cgtatcttgc cgatcggcat
    15241 aatgaaattt ttgccgcttc tcctgcgcta tttttcagcg cgcctggtcg taccgaaatt
    15301 agcggcaatc ataccgatca caatcatggt aaagtactgg cggctagcgt tgacctggat
    15361 tccattgcgg ccgtgaacgc tattaatggg gatcagatta ccattttttc agagggttat
    15421 ccgcaaccat tcatcgttga tatcaatcat tgctcgccgg tcgaagccga gcggggcacg
    15481 accaccgctc tggtacgcgg gatagttgcc cgctttaaag agcttggtct gcgggttggg
    15541 ggcttcaacg ccacagtaac cagcaatgtg ctggtcggat ctggcttgag ttcatcggca
    15601 tctatcgaag ttctgctcgg tacgataatg aattaccttt tcaacgatgg acaaatttcg
    15661 gctgaggata tcgctttgat cggtcagttt gccgaaaatc aatactttgg caaaccctgc
    15721 ggcttgatgg atcaattggc aattgccgtc ggcggcgtgg tggcaattga ttttaaaaat
    15781 ccgtccaagc ctatcattca gaaagttaat tatgatcccg aacagcagaa ttacagctgg
    15841 ctaatcgtta atacgggaac cagccacgtc aacctgactg aggaatacgc cgcaatccct
    15901 cgcgaaatgc gggcagttgc ccgagcgtta ggcgggcagg tttgtcgtga tataacaatg
    15961 caaaatttga ttgaggaaat tccgaatttg cgtcccaagg tcggcgaccg ggcaatcctg
    16021 cgggcttttc attttttgac tgaaaacgag cgggttcagc aacaaatatc tgctttagag
    16081 aagggtgatt tcgccgcctt tctggagctg gccaatgaat ccggcacttc atcttgccgg
    16141 tggcttcaaa attgttatcc cacctcgaat cctactgagc aaggcgttcc cttggcactg
    16201 gcgctgactc agtatttctt aaaaacggtt gggaaaaaag gcgcttgtcg gattcaggga
    16261 ggcggtttcg ccggtactat cctagttttt ataccgaatg aagttctgag tgattatctg
    16321 aaattaatca caaaagtctt taacgaaggc agtgtttctc aattgaatat ccgcccgatc
    16381 ggcgcggttt gccttaataa gatttaaatt ttttgaagcg atcagtttag aagtggacat
    16441 tggtcaacgg ttattagttg ttgggcattg gtgaggtagg tagggcagaa gttaactatt
    16501 cgatattata tcacccctaa ttcatattgt caaacgtaaa tattcgtccg agattttttt
    16561 taaaaatcat ggtaaattct ttcataaaat ctttatcacc aactccgcta tattattaaa
    16621 ttccagtcgc tgtgttgtat tttaaggtta gttatcacag attattaatc ttctcggtag
    16681 gtctattaat cgcgataatt tgccgggcaa tggctatgct tgataccgat tctgatgtta
    16741 aaaatttaac aattctcagc gacaccaatc cgagattact cacagacggc acactttaca
    16801 ccgacttaca actttctcct ccggatgaag attatttaga attggtttcc accaagacga
    16861 tcaatgattc agtcggtatg acgacaaaat ccaatcaatt ggacagtatt gtaaataata
    16921 caggcaatct acccccatat ccgccctatg atccttcacc gacgcgccaa cagatcctac
    16981 agccaggtgg ctatctggaa tggatgatgg gcgaagaccc taatggcgat gaaatctctt
    17041 tttgtgaaat aatcattcgc aacgaactgg atcaggatca gatgattaca gtagagttaa
    17101 acgattctat attagaaaaa gcacgcgaag gtagcgtcga gggcttttct tacgaataca
    17161 atggccaagt gcgttttggc ttaaagcgaa ttcaagataa agattttttt cgagacggct
    17221 gggtttatag ctggagtgtc cgggttagtg acaaatgggg cgggacagca gaatccaact
    17281 ggaccgaggc tactttccgt ttcgacgacg ggattaatca accccctctg ccaccagttg
    17341 agggttttaa tccggatggc attatcgtca cgagtcacta tctggaatta aagtggtctc
    17401 cggctactga tcctgatgta gctgatcgtt tacgctacga aattgtatta agtcgcgatc
    17461 gctcgttcgg cggccgaacc tatattttac taaaaagtgc ctatgaccag aatcaaatag
    17521 tggttcaaac tcctctgctg gagaattgtc aatatttctg gcgtgtgcgt tccatcgatc
    17581 tggctgagga acgctccgtc tggtccaaat ccaatacctt ttgggtcaat cagattaatg
    17641 aagctcctca gcgaccggta cgaattatcc atccacataa tctaaacgaa attacaccga
    17701 attcgtattt ctggtggtta ccttctgatg atcctgatcc cggcgatcag ttaaattatc
    17761 aaattgaatg tagtgaatct cttaacttca ctgaacccat attttcctac cggattccca
    17821 gaactattac tgcgaaggag gtagaaacta aacttgaact gccacaaagg gctattggag
    17881 tctgtcttga tgaaatctcg gcgatcagcc ttttgaaaga taatacgctg tattactggc
    17941 gcattcgggc ttttgatcaa tccgaaatta gtggaacatc agccagtcgt ccggtcagag
    18001 tggcattcaa ttatcaaaat gatccacctc tgccggttgt taaaggtttc tatcctgctg
    18061 atgacgctat catcaaaacc ctgcgcccgg aaatccgctg ggcaaatagt agtgatcctg
    18121 attttagtga ttttcaagcc agcctaactt accagttaga aatcagcaga gacccccagt
    18181 tccccgaaaa tgcatgttcg gtctatacaa ctgaaccggg gcagactgtt tttactcttc
    18241 ctgaaaattt aatagaaaac gaacgctggt tttatcgtgt tcgagcttgt gatcaacacg
    18301 gcagttactc gctctggtcg cctaccaact ccttcattac taatgccata agtgaggcgc
    18361 cctatcctgt tacggatggt tttttaccga aagattcagc ggtggtggaa actgctcagc
    18421 cgctaattag ctggttgccg tccgatgatc ccgacccgga tcaagatgaa cgcgatttat
    18481 attaccttgt tcgtttctac gaggctgata atcccaaaaa ggttagtcag gttgccagca
    18541 aaaccggcat aaccagtctc cacctcccag aattaaaaga ggataaatat tatttttacc
    18601 aaatctcagc ggttgatcca gatggcaatc gcagtgaatg gagtcggctg gtgtgttttg
    18661 gagtaaatgc tgttgatcta ccacctagcc catttgcctt actcgcacca tttaacgggc
    18721 aggattcggt tgcgctggat gccggattcg tctggcatat cgcccacgac aaagatccag
    18781 gctcgcagat caattataca ttatactacg gaaccgatag cctttttttg acgaacttcc
    18841 gtgaaattat gtttgagcaa ccggaagaag atacaattat ggtttataat ccacccgaac
    18901 gtcttaacta cgccactcgc tatttctgga aagttgtggc tactgacaac cgcggcaatc
    18961 aatgctgggc ttctcagact aaccaacgcc cgttcgtttt cacaaccgtt ggtacccaac
    19021 gctcgatcgc cagtatattt aagaatttcc gcttacatca gaattatccc aatcccttta
    19081 acatagagac tttgattaga tatgatgttc cattttatgg acctgttgat gtatcaattt
    19141 acgatttaat cgggatgaag gtcactacgc tagctaccgg caatcatgcg gctggcagtt
    19201 acagtgttta ttggaatggt accgaccaga acggtacgct ggtcgctaac ggtgtttata
    19261 tctgccgctt gactgcgccc ggctatatgt cccatatcaa agtcgtactt atgcgctaat
    19321 ctccagaaat tatctaatct ctttgctacc atattacatc ctggttttag tcataatctt
    19381 ggcttgtttt aggttaagtt tggaatatag ccgttttgac taatgatcaa tgaccagccc
    19441 caccctaacg cagatagtct gatgcaactt gtgttaaggg tattgggctt ttcattccta
    19501 accttagcct cgttcaatct tggtctattt tactaaaata tctgtgtgcc ttagagcctt
    19561 tgttgttaaa ggagcctttt tgcacccaaa tcctagaagg caataaatgc tattttttat
    19621 cagtcaatcg ggatttcatt tcgtcaacta attcttgcac ttccgccaca cgattgcgga
    19681 ttttttgaat aagttcggct tcccgttctt tgggattgcc attttcagat tcggtcgtca
    19741 ctatattaat tctccaaaat atttaaccgt aaataattac cattctgtaa ttacataagg
    19801 aattttaact atttgccaat tcacgagtaa taatttaaaa atccacaact aaaatgccgc
    19861 catttttttg tagctaaaag aagtaataca aaattaatcg aataattgcc caataaaatt
    19921 aataccttcc ttgtcactct tcgacaggct cagagtgaca gtatggctca gagtgacact
    19981 ccatctgtca tcctgagctt gtcgaaggat gagctcgtcg cttcttgtca tcctgagctt
    20041 gtcgaaggat gagtccctca agagacttta ggtcagcgat cttgtcactc ttcgaca
//
LOCUS       DAOOMU010000037        20038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2677_length_20038_cov_18.803333, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000037 DAOOMU010000000
VERSION     DAOOMU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 20038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20038
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2677_length_20038_cov_18.803333"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..352
                     /locus_tag="PLI55_05085"
     CDS             77..352
                     /locus_tag="PLI55_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HPB00240.1"
                     /translation="MDSTKRKLSILFVIGILLLGAMIGSLLGELLRSLIPDGVVKEVF
                     LRSIDIMLGPAVIDLIMFSITLGFTLKLNFIGIIGLAVAYYILRFWR"
     gene            367..594
                     /gene="tatA"
                     /locus_tag="PLI55_05090"
     CDS             367..594
                     /gene="tatA"
                     /locus_tag="PLI55_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPB00241.1"
                     /translation="MGNIGTGEIILILIVFLLLFGAKRLPELAKSIGKSLREFKKAAS
                     DIQDEIKDVDSKPKNNSGNSTNPTEQPTPKE"
     gene            600..1976
                     /gene="gatA"
                     /locus_tag="PLI55_05095"
     CDS             600..1976
                     /gene="gatA"
                     /locus_tag="PLI55_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007502908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPB00242.1"
                     /translation="MAGTVFLLGIEHLLERIKPNPYNAFISVAPDIAEKYDEINYKIQ
                     NGKAGSLAGYTIGIKDNICMVGLPTTCASRMLTNFMPPYEATVVTKIRQADGLIVGKM
                     NMDEFAMGSTTEFSAFGLVNNPHNEKRVPGGSSGGSAAAVAGNLVDIALGSDTGGSIR
                     QPASFCGVIGLKPTYGRVSRYGLVAFASSLDQIGTFSRNVADSARLLQVIAGRDENDS
                     TTADEPVPNYQNALGKDVRNLRIGVPTEYFSKGLDPEIANRIKQCIQFLEKSGAIVTD
                     ITLPHTPYAIATYYIIATAEASSNLARYDGIRYGLSQRGGDLDTLYRETRHAGFGAEV
                     TRRIMLGTFVLSAGYYEAYYDKAQRVRRLIKEDFVEAFKKVDAILTPTTPTTAYEIGS
                     KIDDPLAMYLGDIYTVPANLAGVPALNFPLGKSSEGLPIGAQLIGRYFDEETILRIAH
                     YIERNYEE"
     gene            1995..>2365
                     /locus_tag="PLI55_05100"
     CDS             1995..>2365
                     /locus_tag="PLI55_05100"
                     /inference="COORDINATES: protein motif:HMM:NF019220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HPB00243.1"
                     /translation="MFYFANWGFLVLLILVPVLAIWYWKRGHKNAGALRYSSIKNILQ
                     VKNRHAFYKVHGPFVAKLIGIALLIMALARPQSGNTRREFTTEGIDIMLVLDISSSMK
                     AIDFRPNRLEAAKSVAQKFIEG"
     assembly_gap    2366..2465
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2466..2849
                     /locus_tag="PLI55_05105"
     CDS             <2466..2849
                     /locus_tag="PLI55_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotolerance regulator BatA"
                     /protein_id="HPB00244.1"
                     /translation="EIDPRTAANLANAFDIKIYTIGAGKLGQAPYPIELPNGQIQYRL
                     VNAEIDEVLLQDIADKTGGKYFRATDEKSLAMIYEEISGMEKTEVKVHEFVNYNELYP
                     YFLVPGFLLVALSGLIGLTVWRRTP"
     gene            2849..3874
                     /locus_tag="PLI55_05110"
     CDS             2849..3874
                     /locus_tag="PLI55_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPB00245.1"
                     /translation="MVKFYHSEFLWFWSLIILAILALIYNRIQHRKSLQQYAGIELSQ
                     KLLEGYRPGLRRLKNNLWLVGLVFLGIALLGPAVGQKLTEVKRRGQDIIIALDTSISM
                     KAEDVKPNRLERAKFELNRFIDGLMGDRIGIVAFAGTSYLQCPLTLDYSAAKLFLDAC
                     DTGIIGTQGTAIADAISTSLKAFKSGEKKHKVIILISDGEDHEGDINQIAKQAAEEGV
                     VIYAVGIGSLTGAPIPLRNITTGEVEFKKDRAGHIVMTALEEETMQKIAALTGGKYYN
                     LSANKDAFQKIAHEISGMDKKNLQSHEYSDYQQRYQIFLLIGLVLLTTEILIPEKRNS
                     TSRDTNS"
     gene            3881..4600
                     /locus_tag="PLI55_05115"
     CDS             3881..4600
                     /locus_tag="PLI55_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00246.1"
                     /translation="MTRSKSISNCISRLSLFALGVVILCPLKISAADSGLRQYKKGNY
                     QEAIRAYDRILKDHDDWEEAHFGKGAALYKSQRIEEARREFEKAISVKDPMRKSAVYY
                     NLGNTLFQSGRIEESLAFYKKALEYNPRDFDAKYNYELARQILQQQKNQDKNQSEQKP
                     DQQNQPEEQPQPDQQSQPQNKEQQEQQAQQQPSQQNRQEKEKSQKEAAQILDALKNDE
                     KKLMQEKMRMKASGLAKEKDW"
     gene            4608..6437
                     /locus_tag="PLI55_05120"
     CDS             4608..6437
                     /locus_tag="PLI55_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPB00247.1"
                     /translation="MNRLKTFSLLFFLQLICLHALGQDLRISASVNRNNTTDKDVLIF
                     SVTLEGISDFPEVPPPESPDFVLISGPSQSSSIQIINGKMSASKTVSWQIAPTRAGKL
                     TINPISIKYRGKIYSTEPVIVNVTASAPAQPRSAPPSGTPDKQVTPSSGEEVFLKAVV
                     PKTTIYMGEELIVSFDLYFKNVRTFGRKKLPDAQGFWKEELDAPTQPVITNETVNGVA
                     YRKATIQQIALFATTTGELTIDPMVVDCEVIQPSQRRSIFDDFFGGSFFDDPMFSKTK
                     IVTVQSEPIKIRVKPLPESGQPASFIGAVGQFKIESKIDTLQVKQDQALTLRYKISGS
                     GNINSIKLPPPSLPNSVEVFEPKIEKKIDKSSGSIKGSVTYEYVLIPRRPGLLTVPAI
                     WFSYFDPQTETYRTISTSSFKVKVVGSEDMLSERSVGLNKTEVSLLGQDIRFIMRENP
                     SWHKSGQTVFSEFWFWLINGFSVLVIIGAITLRWWTEKMESNLTFARRRQAWSKWQNR
                     LRTLQKMADNVVGEDFYAQLSQAVNGYIADRLGLPLAGLGPKEIEQELRQYAIDSELI
                     TKTVKLLEKLDEIRFLPGMIIADDPKEMLSSSREIVSALSKVI"
     gene            6439..7203
                     /locus_tag="PLI55_05125"
     CDS             6439..7203
                     /locus_tag="PLI55_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00248.1"
                     /translation="MKYLRLLILILSLTVGLIAENPAELDSLFEQGNRAYQEENYPEA
                     IRLYESIIARGYESGPLYFNLANAYYRVNNIGRAILNYERAARLMPNDPNVAFNLQLA
                     NLSVKDRVEAPPPFFLFRWYQNILNLFSTRGWAVIFSILLLIAASSFAVWLNLDLRRL
                     RRGIKSLFIVSGALSIIVALILFQKYRIETASDFGIVLSGTVSSLSAPQAGSTELFII
                     HEGTKFKILDSDGNWLKIELVDGKQGWVPSADVAKI"
     gene            7278..7760
                     /locus_tag="PLI55_05130"
     CDS             7278..7760
                     /locus_tag="PLI55_05130"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPB00249.1"
                     /translation="MKNKAIVVLILLSFACLLAQENIAPQDESFDPAVLKEPPLPMLD
                     NTLIYEVVTDLKPLTKPPVNNAADSIVYIEKMGWKVQVFSTSDYFLADSIYHEIRSQF
                     GDSTVEKVFNLPYYKIRIGNCATREEAERLLVRAQELGYRDAWVIRTQVKLAVKEGLF
                     "
     gene            7791..8549
                     /locus_tag="PLI55_05135"
     CDS             7791..8549
                     /locus_tag="PLI55_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB00250.1"
                     /translation="MSGHSKWSTIKRKKGALDAKRGKVFTKIIKEIQVAAKMGGGDEN
                     ANPRLRTAVLAAKAANMPLANVERAIKKGTGEIESVNYEEITYEAYGPGGVAILMEAL
                     TDNKNRTVSELRHILTRHGGNLATTGSVAYNFEKKGIITVNKDTCTEDDLLVIALEAG
                     ADDISTEDDVYEVSVDPSHFEKVKKALESAKIAIASAAVTMIPKTTIKVDEKNAPKII
                     SLMDALEDHDDIQNVYANFDIDDKLLAELQQSAG"
     gene            8549..9040
                     /gene="ruvC"
                     /locus_tag="PLI55_05140"
     CDS             8549..9040
                     /gene="ruvC"
                     /locus_tag="PLI55_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPB00251.1"
                     /translation="MSEELRVIGVDHGINRTGYAIVSKNGSKLSCLTHGSIVTTPREA
                     FNLRLKHIYTELLQIIEAWKPTIMVVEEAIYAQNIKTALLMGHARGAVLLAGANAGLT
                     IAEYPPTKIKSAVVGNGLASKEQVQYMVSRILGLSQEPESFDAADAMAAAICHLNRPN
                     IRD"
     gene            9044..9637
                     /gene="ruvA"
                     /locus_tag="PLI55_05145"
     CDS             9044..9637
                     /gene="ruvA"
                     /locus_tag="PLI55_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPB00252.1"
                     /translation="MFAYISGKLFQKEPTSVIIDVNGIGYQINIPLSTYSALPATGQN
                     AKLLTYYHVREDIQALYGFATPEEKELFLLLISVSGIGPKMAMTILSGTSPEQFRRRI
                     LDGDVESLTLIPGIGLKTARRIIVELGEKIGQTSEVVSGELATVTLGATGEEALRALI
                     SLGYRRAEALNALRKAVKELGDSAPVEKYIKAALNQM"
     gene            9647..10753
                     /gene="gcvT"
                     /locus_tag="PLI55_05150"
     CDS             9647..10753
                     /gene="gcvT"
                     /locus_tag="PLI55_05150"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPB00253.1"
                     /translation="MESANNQVKKTPFYDQHLACNAKLVPFAGYWMPIQYRGIMEEHR
                     RVRTTVGVFDVSHMGEFIVRGSSAEDFLNYITINDVSKLAIGQVQYSAMCYPDGGIVD
                     DLLVYRFADHFMLVVNAANLEKDFNWIKQHLPAGVELTDISDETGLLAVQGPLALETL
                     QPLTPINLNEIEFYHFREGEIANVPAILSRTGYTGEKGLEIYHKPADSARLWEAIFQA
                     GAKYDIQPIGLGARDTLRLEMKYALYGNDIDQTTNPLEAGLGWITKLDKPDFIGKAAL
                     LKIKAEGLKRKSVGFEMLEPGIPRHGYKVFKNGQEIGLVTSGTQSPTLNKGIGIAYVA
                     SEYSAVGTPIEIEIRGKLLKAVIVKTPFIPARTQ"
     gene            10750..12966
                     /locus_tag="PLI55_05155"
     CDS             10750..12966
                     /locus_tag="PLI55_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPB00254.1"
                     /translation="MNNRQQEILGILLIALGILIMLSLISYNPIEEPTISKNVAIQNW
                     MGIVGVYISHYLIKYTLGVAVFIFPVLMLVWGWWLFADKERKILIRFSLYSIFMALLV
                     SVALGLPAVRENVDSNFGFRYSGLVGGVFAKVMCDWLGFTGTVIVLLVLALVTIRGYF
                     TWSFYSPIAKWRSHRHPKKERTSGEIPQPRIPERQFSVSQSEKDAAVVQSETEATRQV
                     KIKKSVEPQPPIIEPKVEPVTKSETSGGVYQLPPIDLLELPPESRDSVTKEEMAENAQ
                     LLENALETFGVKGKVVNVSPGPIITRYEIEPAVGVRVSKIAALADDLARILSAKRIRI
                     VAPIPGKSVVGVEIPNRNPEIVYLRSIIGSRQFASAKSKLTIALGKTTSGEIYTLDLA
                     RMPHLLIAGATGSGKSVCINTIIASLLYLARPDEVKFMLIDPKKLELSIFKALEGYHL
                     ITCEDLDEDVITRADNAIVALRSVELEMERRYEILASATVRNIDEYNNRVVSGELDKE
                     FLPYIIIVIDELADLMLTSAKEVEEPITRLAQMARAVGIHLIIATQRPSVDVITGVIK
                     ANFPARIAFQVASKIDSRTILDLNGAEKLLGRGDLLITTPQNPEPFRVHSAYISLEEI
                     ERILEHIENQPRPGQVELPSVKEESVEEFGLYDDSEKDPLYYEALRLVVTHQQGSISL
                     LQRRLKIGYSRAARLIDELEKASIVGAFTGSKAREVLVDEDYLEQLNKTQNDEDEV"
     gene            13020..13670
                     /locus_tag="PLI55_05160"
     CDS             13020..13670
                     /locus_tag="PLI55_05160"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPB00255.1"
                     /translation="MKLVTRKYIFNSIILSVLLTIPILLLAQETTQVILKKVAHCYQS
                     AVPFKIGFTVKLIMPNNANLSKYSGIFFLGSDNQFRVSQTEEEIVYDGQWLWTYDKIN
                     RQVIVEEFNPRSSLKLIWDILSGSFDGYQISKSECQNGLLAVVELKPSSKDGYIRSLQ
                     MKIDRERGRVVSAEYRDFQDNLVNIELDTLIALGRADTGLFKINLNEGDELIDLRP"
     gene            13667..14689
                     /locus_tag="PLI55_05165"
     CDS             13667..14689
                     /locus_tag="PLI55_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPB00256.1"
                     /translation="MKNNSSNIWKKLFSPKTLFAFAISILGLYLGFRKFDSSEFLSYL
                     RLTNLWIFGLSMAVMVFLIGIRGWRWKYLILPINDLPVKQVFEMEMIGYFGNHVFPFR
                     MGEILRGYALSKTQNIPVSAVFGTIVLERTLDVLTFIIVLISGAIFFPGMPKWVRTSG
                     LISGIVILILFILVYISNLKKVALKRFFKQKMLKIKNLDDSKIGKTVKNFWQGLTTLR
                     ETPHLGLIIFQSFLIWAVNIGNFWLMGASLQVYFDLFDLLLVFFVTSAIISVPSAPGY
                     VGTYHAATIGILVFLGLELSKAQAVAVIMHAVGFISLTTLGLFYFLKYQLNFKSTVEV
                     YVEKGA"
     gene            14699..17098
                     /locus_tag="PLI55_05170"
     CDS             14699..17098
                     /locus_tag="PLI55_05170"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPB00257.1"
                     /translation="METMNGNIKTGIPERSFTETEKEVSLQDYFRIIYRGRWIIGIVF
                     VVVMLLTIYITFTTPPEYQASTTLMLSGRAASTSTLFQNPLLPMSYMKVNNEIEVLKS
                     TALARNVIKALRNSPYADSLYLLETRKYHKKGVGLGDLMGGITSGLQHIFMPSQEEDL
                     MVVDTLDRAIVKNLMTSMKVEPIRETEAIRLTISSVDPIEAALIANTIASEYYKTDVE
                     YNLGEVIEMKNFLEEQLEKIEKDLKESEEALKNYQRQEGIFGLDETAKILIDQLSTFE
                     ATYYTTLADLKIAQQTLDNQQRMLTEREQMVIQESVNTTNPFVAQLRQSIAEMEAEKI
                     TVMTVQGYSADHPAIKELDKRITDLKQKLFEESEKITNLGLTPEEQSKISAELLNKVI
                     MNNIEVISLEAKCNEYKKLVDQYSARLDRLPASTLEYARLDRERQVNEKYYILMKTKL
                     QESRITEASKISNVRIIDPAIPPEKPVKPKKKMNLLLGLFLGLGAGVGIAFIRDYLDH
                     SIKSGEDLQRLGLTAIAIIPTIDVKSAVEKIGKVYKDKNNGEEVLVKARLVSHYDPKS
                     AVAEAYRTLRTNIQFMSPDRPIKSLLVTSAGPGDGKSTTVINIAITFANLGKKVLLID
                     ADLRKPIQHKVFEFQRAPGLTHLLIENLPDEEVIHQTEVPNLDLITCGDIPPNPSELL
                     ASQRLKNFMERAREKYDIILFDTPPVIAVTDATVLSQLTDAILLVVRAGSTDLRVLER
                     SLELLRQVKANLVGAVLNGVNMSTGYDSYYYYYRYYYYYSDTGAKKKRASRTRRHHHQ
                     A"
     gene            17160..17969
                     /locus_tag="PLI55_05175"
     CDS             17160..17969
                     /locus_tag="PLI55_05175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014963730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPB00258.1"
                     /translation="MSGRTKSEDFQLILAPDHIYNIDCVIGMQKMPQDFVDLIITDPP
                     FAINFKAQRNNYHRTPSRVLEGYTEIPADEYLNFTLKWMSQAYRILKPSGSMYVFSGW
                     NNLKEVLIALDQVGFVTVNHLIWQYQFGVVTRRRFVTSHYHCLYVCKNDRQRKFFPYS
                     RFDKDARDLSGSSLHYRDKEDVWYIKREYWTGKEKTPTKLPTEVIKKILQYSSSEDDL
                     VCDPFLGSGQVAVVSKMLKRHYIGFEINPVYYEFALKRLMPDNDLFNARAV"
     gene            18094..19236
                     /locus_tag="PLI55_05180"
     CDS             18094..19236
                     /locus_tag="PLI55_05180"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPB00259.1"
                     /translation="MNAESRFLRAVCRVALDETENQIAVDNVVFNEDKLYNLGLYHQI
                     LPLIADYRVTLSKIFPAISATFFEKCRINALESAGRLIVYEKFLANLDQLFQAASIEY
                     RLFKGPVLAYELYEFPYQRPFGDLDILVQPESLEKVHTILSELGCELDDDLYTNFPAE
                     IIKKYRFARHYISVKNPLVAIDVHTGLSSRLHPFHFDFADFWSHSRIIKIGDREYQTF
                     DLSHQALYLLYHAFKHYYFKLIWIIDCRQNTKLPDFDKADFQHLIKTYNLTRVWNIYS
                     RVSSDLWNDSPQLFEYPKVRCSGLSYKINTEVILQGGLFTSLARARILLPLIYLPRLS
                     QKLAYLWRQMFPPRAVIWDFYTSPNLKPTWRNYLRLRYKAVCEIFR"
     gene            19716..>20038
                     /locus_tag="PLI55_05185"
     CDS             19716..>20038
                     /locus_tag="PLI55_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB00260.1"
                     /translation="MKVPLLDLKAQLTTIRSEMLNAITAVLDSTQYIMGPPVADLEEK
                     IADYCGVQFAVGVSSGTDALLVALMALDVKPGDLVITTPYSFFATAGVIARLGARPVF
                     VDIDPR"
BASE COUNT         5710 a         4028 c         4512 g         5688 t
ORIGIN      
        1 gaacgggcgg cggaacaggt gctcggttca gtggacggat tggggatttt taaatctttg
       61 ctagggaaag attaagatgg attcgacaaa acgcaaactt tcgattttat tcgtgattgg
      121 tattctatta ctgggtgcaa tgatcggttc tctgctcggt gaattactgc gctcgttaat
      181 tcccgatgga gtagtcaaag aagtttttct acggtcaatt gatattatgc ttggaccagc
      241 tgtaattgat ctcataatgt tttcaattac gttgggcttt accttgaaat taaattttat
      301 cgggataatt gggctggctg tagcctatta tatcctgaga ttttggagat aactaaagga
      361 gtcaaaatgg gaaacatagg aactggcgaa ataattctta ttttaattgt tttcttgctt
      421 ttgtttggcg ctaaacgctt gcctgaatta gccaaaagta ttggcaagag cttacgcgaa
      481 ttcaaaaaag ccgccagcga tattcaagat gaaataaagg atgtagattc caaaccgaaa
      541 aataattcgg gaaattcaac caatccgact gaacaaccga cacccaagga ataaaacggg
      601 tggctggtac agtttttcta ttaggaatcg agcatttact cgagcggatt aagcctaatc
      661 cgtacaatgc ttttatctca gttgcgccgg atattgccga aaaatatgat gaaataaatt
      721 ataagattca aaatggcaaa gccggctcat tagccggata taccatcgga attaaagata
      781 acatctgtat ggttggtttg ccgacgacct gtgcttcgcg aatgctgact aattttatgc
      841 cgccttatga agccacggtc gttactaaaa ttcgacaggc ggatggattg attgtcggta
      901 aaatgaatat ggacgaattc gcgatgggtt ctacaaccga attctcggct ttcggcttgg
      961 tgaataatcc gcataatgag aagcgagttc cgggtgggtc cagtggtggt tcagccgcgg
     1021 cggtagctgg gaacctggtg gatatagctc tcggttctga cacgggcggt tcgattcgcc
     1081 aaccagcttc tttctgtggt gttattggtc ttaaaccaac ttatggccga gtgtcgcgct
     1141 atgggttggt agcttttgct tcgtctctgg atcagattgg gactttttcg cgcaatgtag
     1201 ccgatagcgc acgcctgtta caggtgatag ccggacgcga tgagaatgat tcgactaccg
     1261 ccgatgaacc agtgccaaat tatcagaatg cgctgggtaa ggatgtccgc aatctgcgca
     1321 tcggcgtgcc gaccgaatat tttagcaagg gactcgatcc cgaaattgcc aaccgcatca
     1381 agcagtgcat tcagtttttg gaaaaatcag gagcgatcgt caccgatatt actttgccgc
     1441 atacgcctta tgccatcgcc acttattata tcattgctac cgccgaagct tcttccaatc
     1501 tggcacgcta tgacggtatc cgatatggtt taagtcaacg cggtggcgat ctcgatactc
     1561 tatatcgcga gaccagacat gccggattcg gagcagaagt tactcgtcgc attatgcttg
     1621 gtacattcgt tttgtcagct ggatattatg aggcatatta cgacaaagct cagcgcgtca
     1681 ggcgcttaat aaaggaagat ttcgttgaag cttttaagaa agtagatgcg attctgacgc
     1741 cgactacacc gacaaccgct tacgaaatcg gttccaaaat tgacgatccg ctggctatgt
     1801 acctcggtga tatttatacc gtaccagcca atttagcggg cgtgccggct ttgaattttc
     1861 cactcggtaa atcgtcggaa ggattaccga ttggtgccca attgattggc cgctatttcg
     1921 atgaagaaac aattttacgg attgctcatt atatagagcg aaattatgag gagtaaccta
     1981 ccagattaag ctgtatgttc tatttcgcaa attggggttt tttagttcta ctaattcttg
     2041 tgccggtgtt ggctatctgg tattggaaaa gaggccacaa aaatgccggc gctctgcgtt
     2101 attcgtcaat taaaaacatt ttacaggtta aaaatcgcca cgccttttat aaagtgcatg
     2161 gtccgtttgt tgccaaacta atcggaattg ctctcctaat aatggcgttg gctcggcctc
     2221 aaagtggcaa taccaggcgg gaatttacca cggagggaat tgatataatg ttggtattgg
     2281 atatatccag ctcgatgaag gctattgatt tccgtcccaa tcggttggaa gccgctaaat
     2341 cggttgccca aaagtttatc gaaggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnngaaat tgacccgcgc accgccgcta atctggcaaa cgccttcgat atcaaaatct
     2521 atacaattgg agccggaaaa ttagggcaag cgccttatcc gattgaattg cccaatggtc
     2581 aaattcaata tcgtctggta aatgctgaaa ttgacgaagt tcttttgcag gatattgccg
     2641 ataaaaccgg cggtaagtat tttcgggcaa ctgacgaaaa aagtctggcg atgatttatg
     2701 aggaaatcag cggaatggaa aaaaccgaag tcaaggttca cgaattcgtc aattataacg
     2761 aactttatcc ttacttttta gtgccgggct ttttattggt tgcgttaagc gggttgatcg
     2821 gattaaccgt ctggcggagg acaccctgat ggtcaagttt tatcattcgg aattcctttg
     2881 gttctggagc ctgataattc tggcgatact ggcactcatt tataaccgaa ttcagcatcg
     2941 taagagcctc caacagtatg ccggaattga actttctcag aaattgttag aaggctatcg
     3001 cccgggatta aggcgtctaa aaaataatct ttggctcgtc ggactggttt tcttgggaat
     3061 tgcgttgctg ggaccggcgg tcggtcagaa actgactgag gtcaaacgac gcggacagga
     3121 cattattatt gcgctcgata cttccatcag tatgaaggct gaagatgtca aacccaaccg
     3181 tctggaacgc gcaaaatttg agctcaatcg ctttattgat ggtttgatgg gcgatcgaat
     3241 cggtatagtt gcctttgccg gcaccagtta cctgcagtgt cctctgactc tggattatag
     3301 tgcggctaag ttatttctcg atgcgtgtga tactggcata attggtactc agggaaccgc
     3361 catcgctgat gcaattagta ctagtttaaa agcctttaaa tcgggcgaaa aaaagcacaa
     3421 agtcattatc cttatttcgg atggagaaga tcatgaggga gacattaatc aaatcgccaa
     3481 gcaggcggct gaggaaggtg tagttattta tgccgttgga atcggttcgc tcaccggggc
     3541 accgatacct ctgcgcaata tcacgactgg tgaagtagaa ttcaagaaag accgtgccgg
     3601 acatatcgta atgactgccc tagaagagga aaccatgcaa aaaattgcag cgttgacggg
     3661 cggaaaatat tataacctga gtgccaataa agatgccttt cagaaaattg cccacgaaat
     3721 atctggtatg gataagaaga atctgcaatc gcatgaatat tctgattatc agcaacgcta
     3781 tcagatattt ttattaattg gtcttgtgct tttgactact gaaattttaa ttccggaaaa
     3841 gagaaattca acctcacgtg atactaattc ttgaacaaat atgacgagat ctaaatccat
     3901 ttcaaattgt ataagtcggt taagtttatt tgccctggga gtggttatct tatgtccgct
     3961 gaaaatatcg gcggctgatt ctggtctgcg tcagtataaa aaaggcaatt atcaggaagc
     4021 aatccgcgct tatgaccgta ttctgaaaga ccacgacgat tgggaagaag cccattttgg
     4081 caaaggcgcc gccctttata aaagccagcg tatcgaagaa gctaggcgtg aatttgaaaa
     4141 ggctatttcc gtcaaagatc caatgcgaaa atcggcagtc tattataatc tcggaaatac
     4201 cttattccag agtggccgta ttgaagaaag tctggctttt tataaaaaag ccctcgaata
     4261 caatccccgc gatttcgatg ctaaatataa ttatgaattg gctcggcaaa tattgcaaca
     4321 acagaaaaat caagataaaa atcagtcgga gcaaaagccg gatcaacaaa atcagccaga
     4381 agaacaacct caaccggatc aacaatctca gccgcaaaat aaagagcaac aagagcaaca
     4441 ggctcaacaa caaccatctc agcaaaatcg gcaagagaaa gaaaaaagtc aaaaggaagc
     4501 cgcgcaaatt ctggatgcct tgaagaatga tgagaaaaaa ctaatgcagg aaaaaatgcg
     4561 aatgaaggct tctggtctgg ctaaggaaaa agattggtaa ttcctggatg aatagattaa
     4621 aaaccttcag cttactgttt ttcttgcagt taatttgtct gcacgcccta ggtcaagact
     4681 tgcgcatatc cgcctcggtc aaccggaaca atactaccga caaagatgtg ctaattttct
     4741 cggttacgtt ggaaggaatt agtgattttc cggaagttcc gcctcctgaa agtccagact
     4801 ttgttctgat ttctggacca tcacagtcca gcagtattca aatcatcaat ggtaagatga
     4861 gtgcctcgaa aaccgtcagc tggcaaattg cacctacgcg tgccggcaaa ttaacaatta
     4921 atcctatttc gatcaaatat cgcggcaaaa tctattcaac tgaaccggtc attgttaacg
     4981 tcactgcttc cgcgcctgcc caaccccgtt ccgcaccacc ttcgggaact cccgataaac
     5041 aagtgactcc gagtagcggc gaggaagttt tcctaaaagc cgttgtccct aaaactacga
     5101 tatatatggg tgaagaatta atagtttcat ttgatctgta ctttaagaat gtgcgcacct
     5161 tcggccgtaa gaaactaccc gatgctcagg gattctggaa ggaagaactg gacgcgccta
     5221 ctcaaccggt tataactaat gaaaccgtca atggtgttgc ctaccgtaag gcgacaattc
     5281 agcaaatagc ccttttcgca actactaccg gggaattgac cattgatcca atggtggttg
     5341 attgtgaagt aatccagcct agtcaacggc gttcgatttt cgacgacttt tttggcggtt
     5401 cttttttcga tgatccaatg ttttcgaaaa caaagattgt aacagttcaa tcggagccga
     5461 ttaagattcg agtcaaaccg cttcccgaaa gcggccagcc cgcttcattc attggagcag
     5521 ttgggcaatt taaaatcgaa agcaaaattg atactctgca ggtaaaacag gatcaagctt
     5581 tgacacttcg atataaaatt agcggtagtg gtaatattaa ttctattaaa ttaccaccgc
     5641 ccagtctacc caattccgtc gaggtttttg aacctaaaat cgaaaagaaa atagacaagt
     5701 cgagcggaag tattaaaggc tcagtgactt atgaatatgt gctaattccc cgtcggcctg
     5761 gattactaac tgttccagcg atttggtttt cttactttga tccgcaaacc gaaacctacc
     5821 gaactatctc aaccagtagt ttcaaagtca aagtcgttgg aagcgaagat atgctttccg
     5881 agcgaagcgt tgggcttaac aagaccgaag tttcactcct cgggcaggat attcgtttca
     5941 ttatgcggga gaacccaagc tggcataagt ccgggcagac tgttttcagc gaattctggt
     6001 tttggctgat caatggcttt tcggtgctgg ttataattgg tgcaatcact ctgcgctggt
     6061 ggactgaaaa gatggaaagt aatctgactt ttgcgcgacg ccggcaagcc tggtcgaaat
     6121 ggcaaaaccg tctgcggact ttgcaaaaaa tggcggataa tgtcgtcggt gaagattttt
     6181 acgctcaatt gtctcaggcg gtgaacggtt atatcgccga tcggctcggt ttaccgctgg
     6241 ctggtttagg tcccaaggaa attgaacaag aattgagaca gtatgcgatt gattctgaac
     6301 tgataacaaa aaccgtaaaa ttgctcgaga agctggacga aattcgtttt ctaccaggga
     6361 tgataatcgc cgatgatcca aaagaaatgc tatcttccag tcgtgaaatt gtatcggctt
     6421 tgagtaaagt gatttaaaat gaaatacttg cgcctattga ttctgatttt aagtttaacc
     6481 gtcgggctaa tagctgaaaa cccagccgaa ctcgattcat tgtttgaaca aggtaatcga
     6541 gcttaccagg aggaaaacta tccggaagcc attcgcttgt atgagagcat cattgcgcgc
     6601 ggctatgaaa gcggtccact atattttaac ctggcaaatg cttattaccg cgtcaataat
     6661 atcgggcgcg cgattctgaa ttacgagcgc gccgcccgac ttatgcccaa tgatccgaat
     6721 gtggctttta acctgcaatt ggctaatttg agtgtcaaag accgagtcga ggcgccaccg
     6781 ccatttttcc ttttccgctg gtatcaaaat attttgaatc tatttagtac acgtggttgg
     6841 gcggtgatat tctcgatttt actcttaatt gcagctagta gttttgccgt ttggttgaat
     6901 ctggatttaa gacgtctgcg ccgcggaata aagtcacttt ttattgtttc aggagcgctc
     6961 agtattatag tggctttgat cctgttccaa aaatatcgaa tcgagactgc cagcgatttt
     7021 gggatcgttc taagtggaac ggtcagcagt ctatccgccc ctcaagccgg tagcactgag
     7081 ttatttatca ttcacgaagg aaccaagttt aaaattcttg attccgatgg aaattggcta
     7141 aaaatcgagc tagttgacgg gaaacaaggc tgggtaccga gcgctgatgt ggctaaaatc
     7201 taaacgccta agactgttta tctcttttaa aattgcccat aatctccgta tattttggct
     7261 gtgttaattt tacgaatatg aaaaataaag ccatagtagt tttaatttta ttatcatttg
     7321 cttgtttgct agctcaggaa aatatagcac ctcaggatga gagttttgat cccgccgttt
     7381 tgaaggagcc gcctctacca atgctggaca acacgctgat atatgaagtt gttactgatc
     7441 ttaaaccgtt gaccaagccg ccagttaata atgcggctga ttctatcgtt tatatcgaaa
     7501 aaatgggctg gaaagttcag gtgttttcca ctagtgatta tttcctggct gattcaattt
     7561 accatgaaat tcgaagccaa tttggtgatt cgacagttga aaaagtgttc aatttgcctt
     7621 attataaaat ccgaattgga aactgtgcaa cgcgggaaga agccgaacgc ctgttagtcc
     7681 gggctcaaga attgggctat cgggacgcct gggtaatccg cactcaggtt aagctggctg
     7741 ttaaagaagg cttattttaa caaaatatta aaaatgatga ggttttaaat atgtcaggcc
     7801 attcaaaatg gagcacaatt aagcgaaaaa agggggctct cgatgccaag cgcggcaagg
     7861 tttttactaa aattattaag gaaattcaag tcgccgctaa aatgggcggc ggcgatgaaa
     7921 atgctaatcc gcgcctccgc acagcggtct tagcggctaa agcggctaat atgcctttgg
     7981 ctaatgtcga acgagccatc aaaaaaggca ccggtgaaat tgaaagcgtc aattacgaag
     8041 aaatcactta tgaagcctac ggacccggcg gcgtggcaat tctaatggaa gccttaaccg
     8101 ataataaaaa tcgcaccgtt tctgaactgc gccacatttt aacccgccac ggcgggaatt
     8161 tagctaccac cggcagtgtc gcctataatt ttgaaaagaa aggtatcatt accgtgaata
     8221 aagatacttg cacggaagac gatcttctgg tgattgctct ggaggctggc gccgacgata
     8281 ttagcactga agacgacgtt tatgaagtca gcgttgatcc cagtcatttt gaaaaggtaa
     8341 aaaaagcgct ggaatcggct aagattgcca ttgccagcgc cgccgtaacc atgattccta
     8401 aaactactat caaagtagat gaaaaaaacg ctccgaaaat cataagtcta atggatgctc
     8461 tcgaggatca tgacgatatt caaaatgttt acgccaactt cgatattgat gataaattac
     8521 tggctgaatt gcaacagagt gccggataat gtctgaagaa cttagagtaa tcggcgttga
     8581 ccatggtatt aatcgcaccg ggtacgccat tgttagcaaa aacggctcta agctctcttg
     8641 tcttacccat ggctcgatcg tgactactcc tcgtgaagcc tttaatctgc gtttaaaaca
     8701 catttatacg gaattgctcc agattattga agcttggaaa ccgactatca tggtagtgga
     8761 ggaagctatc tatgcccaga acatcaaaac tgcgcttctg atgggacatg ctcggggggc
     8821 agtgttactg gcaggcgcca atgccggctt gacgattgcc gaatatccac ccaccaaaat
     8881 aaagtcggcc gtagttggta acggtttagc atctaaagaa caggtgcaat atatggttag
     8941 tcggatttta ggcctctcgc aagaaccgga atccttcgat gccgctgatg ctatggcggc
     9001 agcaatctgt caccttaacc gtccaaacat cagggattaa agtatgtttg cttatattag
     9061 tggaaaatta ttccagaaag agcctacttc ggtcataatt gatgtcaacg gtattggtta
     9121 tcagattaat attcctttgt caacgtattc cgctctacca gctaccggtc aaaatgccaa
     9181 gttactcact tactatcatg ttcgcgaaga tatccaggct ttgtatggct ttgctactcc
     9241 cgaggaaaaa gagctgtttt tactgctaat cagcgtgtcc ggcatcgggc caaagatggc
     9301 aatgacgatt ctctctggca ctagtcccga acaatttcgg cgtcggatat tagacggtga
     9361 tgtcgaatct ctgaccttaa ttccgggtat tggattaaaa acggcgcgac gaattatcgt
     9421 tgaattggga gagaaaattg gacagacatc tgaggttgtt tccggtgaac ttgcgactgt
     9481 tactttggga gcgactggcg aggaagcatt acgcgccctg atttcgctcg gttaccggcg
     9541 ggcagaagct ctgaatgctc tacgcaaagc tgtcaaggaa ctcggtgact ctgcgcccgt
     9601 tgaaaaatat atcaaagcgg ctcttaatca aatgtaaggc ggaaatatgg aaagcgctaa
     9661 caatcaggta aaaaagaccc cattttatga ccagcacttg gcttgtaacg ccaaacttgt
     9721 gccttttgcc ggttactgga tgccgattca atatcgcggt attatggaag aacaccgccg
     9781 cgtccgaaca acagtcggag ttttcgatgt ttcccatatg ggtgaattca tcgtcagggg
     9841 ttcctccgct gaggactttt tgaattacat tacgataaat gatgtgtcca aattagcaat
     9901 cggtcaagtg caatattctg caatgtgcta tcccgacggt ggaatcgttg atgatttact
     9961 tgtttatcgt tttgctgatc attttatgct ggtcgtcaat gccgccaatc tggagaaaga
    10021 ttttaactgg atcaagcaac atcttcctgc gggagtggaa ttgactgata tttccgatga
    10081 aaccggactt ctggctgttc aaggtccgtt agcccttgag accttacaac cattaacgcc
    10141 aatcaattta aatgaaattg aattttatca ttttcgggaa ggtgagatag caaacgttcc
    10201 ggctatcctg tcgcggactg gttataccgg cgaaaaaggg ctggaaattt atcataaacc
    10261 ggctgacagt gccagacttt gggaggctat ttttcaagcc ggggcaaaat acgatatcca
    10321 accaattgga ttaggggcac gtgatactct ccgcctcgaa atgaaatatg cactctatgg
    10381 caacgacatt gatcagacga ctaatccgct ggaagccgga ctgggctgga tcactaaact
    10441 tgacaaacca gatttcatcg gaaaagcagc gctactcaaa ataaaggcgg aaggcttgaa
    10501 acgtaagagt gtcggctttg aaatgctcga gccaggtatt ccgcgccatg gctataaggt
    10561 ttttaaaaat ggccaggaaa tcggtctagt aaccagtgga acccagtcgc cgactttaaa
    10621 caaaggaatc ggaattgcct atgtcgcttc cgaatatagt gctgtcggaa cgccgattga
    10681 aatcgaaatt cgcggcaaat tactaaaagc tgtaatcgta aaaaccccat tcatcccagc
    10741 gagaacgcag tgaataaccg acagcaggaa atactcggca ttttacttat tgcactcggt
    10801 attttaatta tgctgagctt gatttcctac aatccgattg aagaaccgac tatctcgaaa
    10861 aatgtcgcta tccaaaactg gatgggaatt gtgggagtat atatttccca ttatttgatt
    10921 aaatacaccc tcggagttgc ggttttcatt tttccggtat tgatgttggt ctggggctgg
    10981 tggttatttg ccgataagga acgcaagata ttaatccggt tcagtttata ttctattttt
    11041 atggctctat tagtatcggt agctctggga ctaccggctg ttagagaaaa tgtggacagc
    11101 aactttggtt tccgctatag tggcttggtt ggcggagtct ttgccaaggt aatgtgtgat
    11161 tggctggggt ttaccggcac ggtgattgta ctcttagtgc tggctttggt gacgatccgc
    11221 ggctatttta cttggagttt ttatagcccg atcgctaaat ggcgttcgca tcgtcaccca
    11281 aaaaaggaaa gaacatccgg cgaaatacct caacctcgta ttcctgaacg gcagttttct
    11341 gtatcgcaat cggaaaagga tgccgcagtt gttcaatctg aaaccgaagc tactcgacaa
    11401 gtaaaaatta aaaagtcggt tgaaccacaa ccgccaataa tcgaaccaaa agtcgaacca
    11461 gtaactaaat ctgagacctc aggcggagta tatcaactgc cgcctattga tttactcgaa
    11521 ttaccaccag agtcaagaga ttcggtgact aaggaagaga tggctgaaaa tgcccagttg
    11581 ctggaaaatg cactggaaac cttcggcgta aaaggtaagg tagtcaacgt ttcgccaggt
    11641 ccgattatta ctcgatatga aatcgaacca gccgtcggcg tgcgggtcag taaaatcgcc
    11701 gccttagccg atgatctggc gcggattctt tccgctaaac gcatccggat cgtagctccg
    11761 attccgggta aatcggtggt cggcgtcgag attcccaacc gtaatccgga aattgtctat
    11821 ctgcgcagta taatcggttc acggcaattc gctagcgcta agtcgaaact gaccatcgct
    11881 ctcggaaaaa ccacctctgg cgaaatttat actctggatt tggcgcgtat gcctcatctg
    11941 ctaattgccg gtgcaaccgg ctccggtaaa agtgtctgca ttaacactat tatcgccagc
    12001 cttctttatt tagcccgacc ggatgaagtc aaatttatgc tgattgatcc taaaaaattg
    12061 gaattgtcca ttttcaaagc cctggaaggt tatcatttga tcacatgtga agatctggat
    12121 gaagatgtta ttacccgagc ggataacgcg attgtagcct tgcgttccgt cgaacttgaa
    12181 atggaacggc gctatgaaat tctggcgagt gctaccgtac gcaatattga tgaatataat
    12241 aaccgggtgg tcagtggtga actggataaa gaattcctgc cgtatattat cattgtaatt
    12301 gatgaacttg ccgatttgat gctcacctca gccaaagaag tcgaagaacc gattacgcgc
    12361 ttagctcaga tggcacgggc ggtgggtatt catttgatta ttgccaccca gcgcccgtca
    12421 gttgatgtca tcaccggcgt tatcaaagct aatttccctg ctcgtatcgc ctttcaagtg
    12481 gcttcaaaaa ttgattcgcg aacgattctg gatttaaatg gtgcggaaaa attactgggc
    12541 cgcggcgatt tgttaattac cacacctcaa aatcccgagc ctttccgagt tcacagcgct
    12601 tatatttcac tggaagaaat tgaacgaatt ctggagcata ttgagaacca accccgaccg
    12661 ggtcaggtcg aattaccctc ggtaaaagaa gaaagcgtag aagaattcgg cttatacgat
    12721 gacagcgaaa aagacccgct ctattatgaa gctttgcgtt tagtcgtaac tcatcagcaa
    12781 ggttcgattt ctcttctcca acgccgacta aaaatcggtt attcccgagc cgctcggtta
    12841 attgatgaac tggaaaaagc cagcatcgtc ggggcgttta ccggtagtaa agctcgcgaa
    12901 gttttagtgg atgaagatta tctggagcaa ttgaataaaa ctcaaaatga cgaagacgag
    12961 gtctgaaatt ctgtgataaa tatcaaagct actaactgaa aacttcttaa attattgccg
    13021 tgaaattggt aactcgtaaa tatattttca attctataat cttgtctgtt ttactgacta
    13081 ttccaatatt attattagct caagaaacca ctcaggtgat tcttaaaaag gtcgcgcact
    13141 gttatcaatc agcggtgcca tttaaaatcg ggtttactgt taaactgata atgccgaaca
    13201 atgccaattt gagtaaatac agcggtatct tttttctagg ctctgataac cagtttcgcg
    13261 tgagtcaaac ggaagaggaa atcgtatatg atggtcaatg gctctggacc tacgataaaa
    13321 ttaatcggca agtaatagtt gaagaattca atccgcgttc cagtctaaaa ttgatttggg
    13381 atattttaag tggttccttc gacggttatc agattagtaa aagtgagtgc caaaatggtc
    13441 ttttagctgt tgtagaattg aaaccgtctt ctaaagatgg ctatatccgt tccttacaga
    13501 tgaaaattga tcgtgaacgc gggagggtag tatcggctga atatcgtgat tttcaagata
    13561 atttggttaa tattgaatta gatactctga tcgctttggg gcgagccgat accggattat
    13621 tcaaaattaa tttaaatgaa ggcgatgagt taatagattt acggccatga aaaataactc
    13681 cagtaatatt tggaaaaaac tctttagtcc gaagacgtta ttcgcctttg ccatcagtat
    13741 tttgggactt tatttgggct tccgcaaatt tgatagtagc gaatttttaa gttacttgcg
    13801 tctgaccaac ctctggattt ttggattgtc aatggcggtt atggtttttc taatcgggat
    13861 acgcggttgg cgctggaaat atttgatttt accgataaat gatttgcctg ttaaacaggt
    13921 ttttgagatg gagatgatcg gttatttcgg taaccatgtt tttccctttc gtatgggaga
    13981 aattctcagg ggttatgctc tgagtaaaac ccaaaatata ccggttagcg ccgtattcgg
    14041 gactattgta ttggagcgga ctctcgatgt tctgactttt attatagttc taatcagtgg
    14101 agctattttt ttcccgggta tgcctaaatg ggttcgaaca agcggtttaa ttagtggaat
    14161 agttatttta attttattta ttttggtata tatttcaaac cttaaaaaag ttgcactgaa
    14221 aaggttcttc aagcaaaaga tgctaaagat aaaaaattta gatgatagta aaataggcaa
    14281 gacggttaag aatttttggc aaggtttgac gaccttgcga gaaacgccgc atctgggatt
    14341 gattatcttt caatcgttct tgatctgggc ggttaatatc ggcaatttct ggttgatggg
    14401 cgcgtcctta caggtttact tcgatttgtt cgatttatta ctggtttttt ttgttacttc
    14461 tgccatcatt tcagttccgt cggctcccgg ctacgtggga acctatcatg ccgcgacgat
    14521 cggcattctg gtttttctag ggttagaatt gtcgaaagct caagctgtgg cggtaattat
    14581 gcacgcagtc ggttttatta gtctaaccac tttgggtttg ttttattttc ttaaatatca
    14641 attaaatttc aagagtacag tcgaagtgta tgttgagaaa ggagcatagg attactcaat
    14701 ggaaactatg aatggtaata tcaaaacagg aattccggaa agaagcttta ctgaaacaga
    14761 aaaggaagtt tcgcttcagg attattttcg gattatttat cgaggccgct ggatcatcgg
    14821 aatcgttttc gttgtggtta tgctcctcac gatttacatt acatttacca ctccgccgga
    14881 atatcaggca tctacgactc tgatgctctc cggccgagcg gcttctacca gcactttatt
    14941 ccagaatcca ttgttaccga tgagttatat gaaagtcaat aacgagatag aggtgttgaa
    15001 aagcactgct ctagcgcgaa atgtaatcaa agccttacgt aattcgcctt atgctgacag
    15061 tctatacctt ttagaaacaa gaaaatacca taaaaaaggc gttggcttag gagatctgat
    15121 gggcggcata accagcggat tacagcatat ttttatgccg tcccaagagg aagatttgat
    15181 ggtagttgat accctcgatc gagctattgt caaaaattta atgacatcta tgaaagtcga
    15241 accgattcgc gaaactgaag ctattcggtt gacaatttct tccgttgatc ccatagaagc
    15301 cgccctaatt gctaatacga tcgcaagtga atattataag accgatgtag aatacaacct
    15361 tggggaagtg atcgagatga aaaacttcct cgaggaacaa ctcgaaaaaa tagaaaagga
    15421 cctgaaagag tcggaagagg ctttgaaaaa ttaccaacgc caagaaggta ttttcggtct
    15481 ggatgaaact gctaaaattt taattgatca actcagcact tttgaggcta cttattatac
    15541 gactctagct gatctgaaaa tagctcagca aactcttgat aatcaacaga gaatgctaac
    15601 cgaacgcgag caaatggtta tccaggaatc ggtcaatacc actaatccct tcgtagctca
    15661 acttcgtcaa agtattgccg aaatggaagc cgaaaaaatt acggtaatga ccgttcaagg
    15721 ttatagtgct gaccacccgg caatcaaaga gctggataaa agaattacag atttaaagca
    15781 gaagctattt gaagaatctg aaaagattac caatttagga ttgacgccgg aagagcaatc
    15841 taaaatttcg gctgaactac tcaataaggt aataatgaac aatatcgagg tcatttccct
    15901 ggaagccaaa tgtaatgaat ataaaaaatt agttgatcag tattctgccc gacttgatag
    15961 actcccagcc agtactctcg aatatgcccg cctggatcgt gaacgtcagg tgaatgaaaa
    16021 gtactatatc ctgatgaaaa cgaaacttca ggaatcgcgg attaccgaag ccagtaagat
    16081 tagtaatgtt cgcattattg atccagccat tccacccgaa aaaccggtca agcccaaaaa
    16141 gaagatgaat ctgcttttag gtttatttct gggattaggt gcgggagtcg ggattgcatt
    16201 tatacgcgat tatctcgatc attcgattaa atctggtgag gatttacagc ggttaggatt
    16261 gacggcgata gcgatcattc cgaccattga tgtcaaaagc gctgtcgaga aaattggcaa
    16321 agtttataag gataaaaaca acggagagga agtactggtc aaagcgcggt tggtttctca
    16381 ttatgatccc aagtcggccg ttgccgaagc ctaccgcact ctacgcacta atatccagtt
    16441 tatgagtccc gaccgcccaa tcaagagtct gctcgttacc agtgctggcc ccggcgatgg
    16501 caagtccacc acggtcatta atattgctat tacttttgcc aatctgggta agaaagttct
    16561 gctaattgat gccgatttgc gcaagccgat tcaacataag gtattcgaat ttcaacgagc
    16621 tcccggctta acccaccttt taatcgagaa cttgccggat gaagaggtta ttcaccagac
    16681 tgaagttccc aatcttgact taataacttg cggcgatatt ccgcctaatc cttcggaact
    16741 cttagcctct caacgcctta aaaattttat ggagcgagcg agggaaaaat acgacataat
    16801 cctgtttgat actccgccag ttatcgctgt taccgatgcg accgtcctat cgcaacttac
    16861 tgatgcaatt ttactggtgg ttagagctgg ttcgaccgat ttgagagtac ttgagagatc
    16921 actcgaactg ttgaggcagg tcaaagcaaa cctagtcgga gccgtactga atggagtgaa
    16981 tatgtcaact ggctatgatt catactatta ctattatcgc tactattatt actatagcga
    17041 taccggcgcc aagaaaaaga gggcgtcacg taccagaaga catcatcacc aagcctgatt
    17101 atgtgaggct ggctagtcga ttgacaacca agttcacttg gcgattctga taatctgcta
    17161 tgtcgggaag aactaaatcg gaagatttcc aactaatact ggcacctgat catatttata
    17221 atattgattg cgttatcggg atgcaaaaga tgccgcagga ttttgttgat ttgataatta
    17281 ctgacccacc gtttgcgatt aatttcaaag ctcagcggaa taattatcat agaactccgt
    17341 cgcgagtgtt ggagggttat actgaaattc cagcggatga atatctgaat tttacgctca
    17401 aatggatgtc tcaagcttat cggattttga aaccgagcgg gagtatgtac gttttttccg
    17461 gttggaataa tttaaaagaa gtattgatag cgcttgatca agttggtttc gtgaccgtca
    17521 atcatctgat ctggcagtat cagtttggag tagttaccag acggcgcttt gtcacttccc
    17581 attatcattg tctttacgtc tgtaagaatg atcgccagcg caaatttttt ccctatagcc
    17641 gatttgataa agatgcccgc gatttatctg gctcaagttt gcattatcgc gataaagaag
    17701 atgtctggta tatcaagcgc gaatactgga ctggcaagga aaaaacccct accaaattgc
    17761 cgactgaagt catcaaaaaa atcttgcaat attccagttc ggaagacgat ttggtttgcg
    17821 atccattttt agggtcgggg caggtggcag tagtaagtaa aatgttgaaa cgtcactaca
    17881 ttggcttcga gattaatccg gtctattacg agtttgctct taaaagattg atgcctgaca
    17941 atgatctttt taatgctcgg gcggtctgaa tcggcagttt tccataggct tccgtttaac
    18001 taactttcaa taacctttta ttccttttcg tgattattag ctaaaataat ttttatgtgt
    18061 ttttctatct ggttatgtat atatttctaa gcaatgaatg ctgaaagtag gtttttaaga
    18121 gcagtttgta gggttgcttt agatgaaacc gaaaatcaaa ttgccgttga taatgtcgtt
    18181 ttcaatgagg ataaactcta taatctcgga ttataccatc agattctacc gctgattgcc
    18241 gattatcgtg tgactctatc caaaattttc ccagccatct cagccacatt tttcgaaaaa
    18301 tgccgaatta atgctctgga aagtgccggc cgacttatag tttatgaaaa atttctggct
    18361 aatctcgatc aactatttca ggccgcaagc atcgaatacc gtttgttcaa aggaccagtc
    18421 ttagcttatg aattgtatga attcccttat cagcgccctt ttggcgatct ggatatttta
    18481 gttcagcctg aatcactgga aaaagttcat acgatattaa gtgaattagg ttgtgagttg
    18541 gatgatgacc tttacactaa ttttcctgca gaaatcatca agaagtacag atttgcccgc
    18601 cattatattt cggtaaagaa tccattggtg gcgattgatg tgcatactgg cttaagcagt
    18661 cgcttgcacc cgtttcactt cgattttgct gacttttggt cgcattccag aataattaag
    18721 attggcgatc gtgaatatca aactttcgat ctatctcacc aagctcttta tttgctatat
    18781 catgcgttta agcattatta ttttaagttg atttggatta ttgactgccg acaaaatact
    18841 aaattgcctg atttcgataa agccgatttt caacatctaa tcaagacata taacttgaca
    18901 cgtgtatgga atatctattc tcgggtttca tctgatttgt ggaatgactc accgcaattg
    18961 tttgaatatc ctaaagtaag atgttcggga ttgtcttata aaataaatac agaggtaatc
    19021 ttacaggggg gactgtttac ctcgctggca cgagcccgaa tcttactgcc gctgatttat
    19081 ttaccaaggt tgtctcaaaa attagcatat ttatggcggc aaatgtttcc gccccgcgct
    19141 gtgatatggg atttttacac ttctccgaat ttgaagccga cctggcgtaa ctacttgcgg
    19201 ctacgataca aggcggtatg tgaaattttc agatagtaga tcggtggaaa tttcttaaaa
    19261 ttttaccgaa gatttaattg catgtaacaa attttaacga tctatatcaa ttaattcgtt
    19321 ggggactcaa ttttttatat tggaattgat gctatttttt ttgaaattac tttgtttgag
    19381 tgatattgag aaatacaggg aatattgcta actgaaaatg acatgaaatt gacataaaat
    19441 cctttccatt tattgatttt ggaagttggt aatcaccgga agcatatagc gaaatccgtg
    19501 cttttgtgag agaagtcacg acagaagttt agtaattagc tcaaaatatc ctgaaattcg
    19561 atgtatttat ttcttggcct cgtcgctgaa tgtagttgac ggggcggagc ctttggaaag
    19621 gaatcggtaa ctcaggttcg gcgccaaatt ctaaccgcca gtaaataaaa gactaatatc
    19681 tcattgaaat taattactct atttgggaag aaactatgaa agtgcccttg ctcgatttaa
    19741 aagcccaatt aactaccata cggtctgaaa tgctgaatgc cattacggcg gtattggatt
    19801 caacgcaata cattatgggt ccaccggtgg ctgacctcga ggagaagatt gccgattact
    19861 gtggtgtgca atttgccgtt ggcgttagtt ccggaactga cgctttgcta gttgctttaa
    19921 tggctttgga tgttaaaccc ggcgatctgg tcattacgac gccatattct ttttttgcga
    19981 cggctggagt tatcgctcgt ttgggagccc gcccagtttt tgtagatatt gatcctcg
//
LOCUS       DAOOMU010000038        19014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2960_length_19014_cov_13.510470, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000038 DAOOMU010000000
VERSION     DAOOMU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 19014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19014
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2960_length_19014_cov_13.510470"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..805
                     /locus_tag="PLI55_05190"
     CDS             101..805
                     /locus_tag="PLI55_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014772507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HPB00261.1"
                     /translation="MRYRIELLVLIVIFCLFATLCSKKQSEPTSEFNPQVGDLMLQDL
                     DGSPFYEAIEAVTKGYNGASLSHIGIVTKDSSGKYFVLEAVLAGVSLTPLVEFLARSY
                     DNQNHPKVLVGRLKPKYRHQILPALKEAQKLIGKPYDNFFDIENDAYYCSEVIYKAFL
                     KSTDNQSLFKLQPMTFVDPRTGGTFPIWQEYFAELGVAIPEGKLGINPGEISRSKAID
                     IIYNYGIPDGWKKLNK"
     gene            825..1808
                     /locus_tag="PLI55_05195"
     CDS             825..1808
                     /locus_tag="PLI55_05195"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HPB00262.1"
                     /translation="MKQIRIGIVGYGNLGRGVEQAVTQNPDIELVAIFTRRDPAKITT
                     ASKSTMVLSANELASMQSAIDVMILCGGSATDLPKQGPAFASLFNTVDSFDTHAKIPE
                     YFAKVDKAARDNGHLAIISVGWDPGLFSINRLYAEAILPNGKSYTFWGKGVSQGHSDA
                     IRRIKGVKNAIQYTIPIEESLVAVRSGANPQLTTRQMHRRECFVVLEENADKDYIENT
                     IKNMPNYFIENETVVHFISEDEFKKNHRKMNHGGFVIRSGHTGAGNKHIIEYSLKLDS
                     NPEFTGNVLVCYARAAYRLAQSGEIGARTAFDIPPALLSPKSGEQLRNELL"
     gene            1881..2918
                     /locus_tag="PLI55_05200"
     CDS             1881..2918
                     /locus_tag="PLI55_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPB00263.1"
                     /translation="MKAVLLTGIRQFTIQQVADPTIVNDTDVLIRVKMVGVCGSDIHY
                     YKNGKIGSQVIQFPFIIGHEATGIVEKVGNKVTRVKIGQRIAIDPTVYCANCDQCRSG
                     RENTCRNLKFLGTPKQLNGALCEYIVLPENCCYPISDKMTFEQAVLSEPLAIAIYSVA
                     RSRLPDNANVAILGVGPIGMSVFCILRIKNVGNIYVTDKIQERLDFAQQLNPEWSGNP
                     DLTNVVSEINKREPLQLDVVYECSGDTTAISQAVQLLKPGGILVLVGIPEMDEISFPI
                     HELRRKEITIINVRRQVRCTQKAIDLLESNRVNMNAMATHHFPLEKTAEAFELVANYR
                     EGVMKALISID"
     gene            3004..4353
                     /locus_tag="PLI55_05205"
     CDS             3004..4353
                     /locus_tag="PLI55_05205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPB00264.1"
                     /translation="MQKITRKNLTCSNLLKLILILLIIGQFSPILVARDTSRAAYYPD
                     PTQYPDHLILSWSDDPTSTQSVTWRTDTSIKSAYGEIALADASPNFVYQAKRIPAKTE
                     LLNFDGKSANYHSVTFTGLQSNTLYIYRVGSDKRWSEWFQFRTASKKSEPFRFIYLGD
                     GQEDILFLWSRAIRTAYKTAPDARFIIHGGDLVNNARDYPALNEWFTAAGWINATVPI
                     MPAIGNHEYYDGNWGEQFFMPYWRPQFTLPLNGIEELPETNYYFDFQGLRVIVLNSNF
                     ELEKQAQWLEEILKNNPNKWTVVAFHHPIYCGAEGRDYQELRDTWNPIFKKYKVDLIL
                     TGHDHTYARGNNPERLTIRPNDPTGPVYVVSVSGPKLYNINKIRWMDRAAENTQLYQI
                     ITINDNILDYKAYTVTEELYDAFQIIENSKGHKVFFDLQPKNFPERMYKNTLNAPNN"
     gene            4364..4603
                     /locus_tag="PLI55_05210"
     CDS             4364..4603
                     /locus_tag="PLI55_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6429 family protein"
                     /protein_id="HPB00265.1"
                     /translation="MKYDKDKVDNMILALLFLTSSSDQYGTKAWKGFDFEALDRLYQK
                     GLISDPKDKSATLSLSEKGANLSKELFFKYFVIKD"
     gene            4882..5961
                     /locus_tag="PLI55_05215"
     CDS             4882..5961
                     /locus_tag="PLI55_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00266.1"
                     /translation="MYKKIILLMLIFVINASGDDYEYYFYNPDLDYGSESIFNPISLF
                     INGSFDILRNGAHNKNIFDQPYKTGFNNVIDNVLHPLDNIRNYGVKNFLNREIFNLEV
                     NKNESQFLPNIGLHLIGNGMQYRKLAEWYDYHEYPTPNLLSIITTISYQLVNEIVENG
                     SYSGTNVDPISDMLIFNPLGILLFSTKAGQEFFSTKMPIYDWSMNPYFNPNNYYLENA
                     GQQYATKIKLKTTDKNALFGYWGVVSTFGISHTINETDAISFSAGAVVAKLTPNEEYD
                     SRFIIPNLDYAVAFFYDRNNSLLGSLTLFGPKFYNLRLEIFPGIIGFKNYKPGFFIGI
                     GEMDNFQFGISFPPIPIGIIGEISN"
     gene            5974..6987
                     /gene="galE"
                     /locus_tag="PLI55_05220"
     CDS             5974..6987
                     /gene="galE"
                     /locus_tag="PLI55_05220"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPB00267.1"
                     /translation="MKILLTGGAGYIGSHTLVELLNQNHEITVIDNLSNSKIESIKRV
                     KTITGKELIFNKIDLCNIIELEKVFSSNQFEAVIHFAGLKAVGESVQKPLLYYYNNIT
                     GTINLCQMMIKYDVKNIVFSSSATVYGNPTSVPITEDFPIKPANPYGNTKYIIETILK
                     DIQIANPDWNVIILRYFNPVGAHESGLIGEDPNGIPNNLMPYISQVAVGRLPYLKVYG
                     NDYPTKDGTGIRDYIHVVDLAQGHLKALDKLSTKPGYCIYNLGTGQGYSVLDVITTFE
                     KWSQRKIPYKIVGRRPGDIAECYADPSKANRELAWKAERNLDDMCRDTWNWQKKNPNG
                     YSD"
     gene            complement(7022..9256)
                     /gene="purL"
                     /locus_tag="PLI55_05225"
     CDS             complement(7022..9256)
                     /gene="purL"
                     /locus_tag="PLI55_05225"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HPB00268.1"
                     /translation="MASQIEPEVNLQTAIQHGLTEEEWNKILKILGRTPTYTELGIFS
                     VMWSEHCSYKNSIALIKTLPRSGKHLLVNAGEENAGLVDIGDGLAICFKIESHNHPSA
                     VEPFQGAATGVGGILRDIFTMGARPIASLDSLRFGPLEAPRNRYLFDNVVKGIAHYGN
                     CVGVPTVAGEIYFEDCYTENPLVNVMTIGLVKNKNVVSAVAKGEGNTVMIVGSSTGRD
                     GIHGATFASVELTEESENRRSSVQVGDPFTEKLLLEATLELAKQDFLIGIQDLGAAGI
                     ACSTSEMAVRGKSGMIINLDSVPLREPGMSPYEIMLSESQERMLVVIRKGFEEETKAI
                     FEKWDLNCVKIGKVVNSGNLDIFMNNKKVASLPAWHLVLGGGAPVYERETKEPAYLKK
                     VQGRNLKKIPEPKDYNKVLLQILSSPNIADKSWVYRQYDYSVRSNTVVKPGGSAAIIR
                     IKGTRKALAIKTDGNGRYTYLNPYKGGAIAVAESARNVVCSGARPIAITNCLNFGNPY
                     DPEIYYQFKMAVQGIGDACRVFDTPVTGGNVSFYNESPKGAIFPTPVIGMVGLIEDLK
                     NVTTPWFKNDGDFIILLGALKGELGGSEYLKVVHNIIAGDAPDIDLTFEKRIFNATLE
                     AIQKGIVNSAIDISDGGLAVALAEACISNPEKPLGASVYISRKLRNDELFFGESQSVI
                     LISISEKYLLDIERIASKNIIPSVTIGRVKDNGRLTINNIIDLPLTELQKAYTRTIPG
                     FMDI"
     gene            complement(9283..10521)
                     /gene="lysA"
                     /locus_tag="PLI55_05230"
     CDS             complement(9283..10521)
                     /gene="lysA"
                     /locus_tag="PLI55_05230"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPB00269.1"
                     /translation="MKINGLNVSDLADEFGTPLYVYDFDRIKDNAQRLRRAFDSDEIE
                     TAFFYAVKANSHPAIVQLLKDCGYGCDCVSPGEIEIALKTGVKPSDIIFAGNYESMED
                     FNVAVEAGVNIILDDITSLDRLLRIHCPEVISFRINPGKGRGKYEQITTGGEKAKFGV
                     PHEKAWLAYEKARQVGIKRYGAQMMTGSGILDEEHFPRMLELLLNILGQIHQKLGITF
                     EYVDMGGGFGIPYYGDEHELDIQWVGRECLKVLKAKSESLSIGKPALHLEPGRYLVGD
                     AGYLVTRVLGIKESYQKFAGLDAGFNTLIRSALYKAQHPVIVDGKEDEKELYPINLCG
                     QICENTDIFTVGRPLPKLEEGDLLIFTQAGAYGSTMSMTYNHRLRPAEVAIINGKAIE
                     ITRRETMSDYWSRITYPILL"
     gene            complement(10535..12364)
                     /gene="uvrC"
                     /locus_tag="PLI55_05235"
     CDS             complement(10535..12364)
                     /gene="uvrC"
                     /locus_tag="PLI55_05235"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPB00270.1"
                     /translation="MAERQFPEKLLEKIRTAPTDPGCYIYRNSAGKIIYIGKAKNIRN
                     RVRSYFVGGYKEPKTAALVNNIADVEYILTNSEIEALILENTLIKTHHPKYNIFFRDD
                     KTYPYVRITNEPFPQVFMTRSVVRDGSKYFGPYTDSRLLRDTLEIIKNIFPIRSCKHH
                     LNDKTIANGHIKVCLDYHIKKCEGPCQRLVSAEEYNRMINQVIAFLNGRTEESIEYFE
                     EKMKTAAASLQFETAAAYRDKISILMNYSNRQSVELNDGLDRDLIAVLKEAENCCAVI
                     FRVRQGKLIGRDKFLLEGTENYDYPAITHDLLLRYYDNATSIPSEVLLNYLPEEQEML
                     EKWLMSQKGARVSILQPQRGDKARLMKMAIRNAELQISELQMKQSQKTEFVPKSLVAL
                     QNELGLKELPRRIEAFDISNIGGKFAVGSMITFINGAAKSSEYRRFKIKTVTGSNDYA
                     MLAEVVRRRYTRLLREEKPLPDLILIDGGKGQLNAAVTVLQELNLSAIPVIGLAKRLE
                     EIFIPGNDEPILLARNSLALSLLQRVRNEAHRFAITYHRKIRGKGGISTKLDEIPGLS
                     QNTKKALLQHFKTIANIKSASMEDLMNVKGIGPKIAQKIKQYL"
     gene            complement(12444..12626)
                     /locus_tag="PLI55_05240"
     CDS             complement(12444..12626)
                     /locus_tag="PLI55_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00271.1"
                     /translation="MKYCDLSCEYAEFPDKLSDGSATCQTFIAIHCKLLDRLVDKNGI
                     CPAEQSATDKSVNKGR"
     gene            complement(12718..15864)
                     /locus_tag="PLI55_05245"
     CDS             complement(12718..15864)
                     /locus_tag="PLI55_05245"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPB00272.1"
                     /translation="MSLSNLAIKRGITFTMIYLIVIGFGIFGLTQLKLDLYPNITIPV
                     VGVMTQYTGVGPEDIENVITRPLEQTLVAVENVDKIISMSSVGNSLVILQFDWDADID
                     QSEIDVRKNIDLIRDYLPPDASNPITISFDPSMTPVMLISARSGQLGMAELRRVIDDE
                     VRPRIERLKGVASAASGGGLERQIKVLLDPQKVAAYGIPAQQIVQKLQMENVQIPAGV
                     VDDEYQEYSVRTYGEYRTVDQIANTVIGNVSGTPIYLKNVANVIDGYKEQNQIVRNNG
                     EDAVMLFIQKQSDANTVQTARAVAKELPKIMELVGKDVKLEIVFDSSEFITKSLNNLV
                     STGVQAFILVFLVLLFFLRYIRSSLITAISIPVSIIVTFFAMYLLNMTFNVITMAGLA
                     LAIGMLVDNSIVVLENIFRLKEKGLDIRQAADEGANEVSMAITASTLTTIAIFVPVLF
                     LPGISGILFKDLVIVIVSSLTISLLVALTLIPLLSTQWLKGEKKKSKSKVLRRMDSVI
                     DNFLQGLHDRYVRILDYFLGHKKVFLIGLIVIFVITILMFPFLGGEFLPEADRSNIRI
                     SVERETGASLASTDKTFREIEKIIREEVPEAENIQVNFGVGEGVTAIMASSNSGNITV
                     RLPDVDERERSSFEIQDILRERFQKIPGANITIAGGEEATSMLSGGITIKIFGYDRLI
                     ARDLGDQVAEMLKDIKGVVDVTKSYNLPKPEYQIIIDRDKASAFGLSTYQIANIVGTS
                     IRGTRVTRFREGGDEYDVIVQLDEQFRKSQADIENLYITTPTGAQFPLKNIARVLPGA
                     GEESINREDQERRVSVSCSVSGRDIQSVTRDITAGLRNINFPPEFRWEIGGAEEERRE
                     AFLYLYIALAAAVILVYMVMASQFESLLDPFIIIFTIPLAMIGAIWLLFITGTPMSVM
                     ALVGCILLVGIVVNNGIVLVDYINQLRYKHNYHLWIAILAGSKRRLRPVLMTALTTIL
                     GMLPLALGLGTGAELWAPMAIAVIGGLTFATVFTLILIPIIYLFFEQIALKRKIKKGK
                     VEAEPIGRPEGFDINLVK"
     gene            complement(15861..17003)
                     /locus_tag="PLI55_05250"
     CDS             complement(15861..17003)
                     /locus_tag="PLI55_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB00273.1"
                     /translation="MKLSKNILNLFLVCGIIYYVNSCSKTQTDEQMTENIVPVNVVTA
                     ERGTIEEKINFLGNIKGFREIKIYSLIPNKILKINVDVGDWVNKGDVLAVVESDKIEQ
                     AVKQANAGLEAARAQLRNIETEWNRIKKLYDDNAVSKSQYDAITTQRDAARANIKQLE
                     AAVSTAKKQLEDTQIKAPIAGIVSSRLLDVGDQAAPQIPVFTVVEMDDVKITIDVIES
                     QFNKVKIGQKAYINVDTYPDTVFVGEISKISPTLNLMTKTSPAEIIISNPEHKLRPGM
                     FARVDVITDVHKNALIIPRYSIIERTSLEYIGGDISSTKTIINKYVFVVESGIAREVK
                     IETGIENGRIVEVLQGLNDGDLIVSMGQHNLSDSTKVEIIEKGKAI"
     gene            complement(17018..18364)
                     /locus_tag="PLI55_05255"
     CDS             complement(17018..18364)
                     /locus_tag="PLI55_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB00274.1"
                     /translation="MKFKLKIGVVFLITIASLFAEELNLDIETAREIALQRNPSLKLA
                     REAVLKSRASVNEVRGSMLPSISAFSQFKHAWEVGGMPTPQEFKDLITLLGGTPQETM
                     SFERENTFVAGLNLSQPVFTGGLILNSYQMSKIGVRITESQLKATEQKILSDVTNAYY
                     GVLFANSASEVAEEALHSAEQNLSQVQKFFDVGKASRFDVLRAEVQVANFKPMVVSAN
                     NAKKLAESNLRLILGIGEDININYLEKLSYKESDLINMTLEELITIALAERSEVIMLE
                     DRKKLAEKQVNLAKAKYSPSLVFGTAYQYQAFRDDFDFTTDDLVKSFNSSISLSIPIF
                     SGFATKAKVQQAKIAVKEVDYQAESAIDGIKLEVKAAYFKMKEANENVQTQEKTIEQA
                     KEALRLAELMYSEGASTQLDVLNANVALNQAKMNYQKSLYEYNVALANLKKAINQL"
     gene            complement(18396..18983)
                     /locus_tag="PLI55_05260"
     CDS             complement(18396..18983)
                     /locus_tag="PLI55_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPB00275.1"
                     /translation="MKCENITRDKIIKTAAKLFADKGFEATSMREIAELCELTKPALY
                     YHFQDKNTLFAEIINAIANYSVQFLTEIYQSDKDPINKLYDIVIMQVTRLKQNPEITK
                     FLINAPIRNIPNETKVEFYKQMQKNKEMLIHIITEGKEKGYLKSDLDVNAFLYCYIGG
                     INQFIMDHIKNGTENLNEQNANVIVNTLLNGARNH"
BASE COUNT         5708 a         3873 c         3481 g         5952 t
ORIGIN      
        1 atttttcaaa tcagcttgcg gagatttaat attccgagaa attagggatt gtaattttct
       61 tgaaattcgt cgcattgttt cttaaaatag cggcgtgaaa atgagatata gaatagaatt
      121 acttgtattg atcgtgatat tttgtttgtt tgcaacttta tgttctaaaa aacaatcgga
      181 accaactagt gagtttaacc cgcaagttgg cgacctaatg ttacaagatt tagacggcag
      241 tccgttttac gaggcaattg aagcggttac gaaaggctac aacggtgcga gtctcagcca
      301 tataggcatc gtcaccaaag attcgagcgg taaatatttt gtgttggaag ccgtgcttgc
      361 cggagtcagt ttgacgccac tcgttgaatt tttagcgcga agttatgaca atcaaaatca
      421 tcccaaagtc ttagtcggcc gactcaagcc gaaatatcgc caccaaattc taccggcatt
      481 aaaggaagcc caaaaactta ttggtaaacc ttacgacaat tttttcgaca ttgaaaacga
      541 tgcctattat tgctctgaag taatttataa ggcttttctc aaatcgactg ataatcagtc
      601 actttttaaa ctgcagccaa tgacatttgt tgatccaaga acagggggaa cttttccaat
      661 atggcaagag tatttcgccg aactcggtgt agctatccca gaaggaaagt taggtatcaa
      721 tcctggtgaa atatcgcgct caaaagctat tgacatcata tataattatg gaattccaga
      781 cggctggaaa aagctcaaca aataaaaatg aaaggaatat tcctttgaaa caaattagaa
      841 tcggtatcgt cggctatgga aatcttggtc gaggagtgga acaggctgta actcagaatc
      901 cggatattga attagtagcc attttcacca gaagagaccc agctaaaatt acaacagcaa
      961 gtaaatctac tatggtgctg agcgccaatg aattagcaag tatgcaatca gcaattgatg
     1021 tgatgatatt atgtggtggt tcagccacag atttgccgaa gcaaggaccg gctttcgctt
     1081 cgttgtttaa cacagtagat agcttcgata cgcacgccaa gattccggaa tatttcgcta
     1141 aagttgacaa agccgcgcgc gataatggcc atttggctat tatttcggta ggttgggacc
     1201 ccgggttatt ttcaatcaat agattgtatg ccgaggcaat cttgcctaat ggcaaaagtt
     1261 acacattttg gggaaaagga gttagccagg gacattcaga tgcgattcgg cggattaaag
     1321 gtgtcaaaaa tgctatccaa tacaccatac caatcgagga atcacttgtg gcagttcgta
     1381 gtggtgccaa tccgcagttg accactcgtc aaatgcatcg ccgagaatgt tttgtcgtgc
     1441 ttgaagaaaa tgcggataaa gattatatcg aaaatactat aaaaaatatg cccaactatt
     1501 tcattgagaa tgaaacggtt gtccatttta tttccgaaga tgaatttaag aagaatcata
     1561 gaaaaatgaa tcacggtgga tttgtaattc ggagcggaca taccggagca gggaataagc
     1621 acattattga atattcgttg aagttagatt ctaatcctga atttaccgga aatgtattgg
     1681 tttgttatgc ccgtgcggct tatcgtttag cacagagcgg cgaaataggt gcgagaactg
     1741 cgtttgacat tccgccggct ttattgtcac ccaagagtgg cgaacaactg cggaacgagt
     1801 tattgtgatc attcttaaat cattaaaatc tggttggata atcatagata aattttattt
     1861 ttaagataaa attaattata atgaaagcag tattactcac cgggatcaga caattcacca
     1921 tccaacaggt tgccgatcca acaattgtaa atgacaccga tgtattaatt cgagttaaaa
     1981 tggtcggagt ttgtggttca gatatccatt attataaaaa tggcaaaatt ggatcgcagg
     2041 ttattcaatt tccatttatc atcgggcatg aagccactgg aatcgttgaa aaggttggga
     2101 ataaagttac tcgagtcaaa atcggtcagc ggattgccat tgatccgaca gtttattgtg
     2161 ctaattgcga ccagtgtcgc tcaggtcgtg aaaatacctg ccgcaacctg aaattcctgg
     2221 gtactccaaa acaactaaac ggtgctttat gcgagtatat cgtgctcccg gaaaattgtt
     2281 gctatccaat cagtgataaa atgaccttcg agcaggctgt tttatccgaa ccactggcga
     2341 ttgcgattta ttccgtcgca agatcccggc ttccggataa tgctaatgtt gctattctgg
     2401 gagtcggacc gattggtatg tcagtttttt gtatcttgcg aataaaaaat gtcggtaata
     2461 tctatgtaac tgataaaatt caggaacgcc tcgattttgc ccaacaatta aatcctgagt
     2521 ggagtggtaa tccagattta accaatgtag ttagcgaaat taataaacga gagcctctac
     2581 aactggacgt cgtttatgaa tgtagcggag atacaacagc aattagtcag gcggttcaat
     2641 tgcttaaacc aggtggaatt ctagtattgg ttggcatccc tgaaatggat gaaatttcat
     2701 ttccaattca tgaacttcga cgtaaagaaa tcactataat taatgtccgt cgccaagtcc
     2761 gctgcaccca aaaagctatc gatttattag aaagcaatcg ggtaaatatg aatgcaatgg
     2821 cgacgcatca ttttccactt gaaaaaactg ccgaagcatt cgaactggtt gctaattatc
     2881 gggaaggtgt aatgaaagca ctaatttcca tagattaatg ttttttgatt tatttagatg
     2941 atatttaagg ttatgtaata taattaaatg aaagggtaga ggcacaaaag gagcaatttt
     3001 atcatgcaga aaatcaccag aaaaaatttg acttgcagta atttattaaa attgatattg
     3061 attttattga taatcgggca attctctcca attttagttg ctcgggacac atcacgggcg
     3121 gcttattatc ccgatccgac tcaatatccc gaccatctaa ttctttcctg gagtgatgat
     3181 cccaccagca ctcaatcggt aacctggcgc acagacacca gcattaaatc tgcttatggt
     3241 gaaatagcgc tagccgatgc atcgcccaac tttgtttatc aagccaagcg aatacctgcc
     3301 aagaccgagc tacttaattt cgatggaaaa agcgccaatt atcattcggt aacttttacc
     3361 ggtttgcaat caaatacatt gtatatttac cgggttggtt cagacaaacg atggagtgag
     3421 tggtttcaat ttcgcactgc gagtaaaaaa tcagaaccat ttcgatttat ttacttaggc
     3481 gatgggcaag aagatatact ttttctatgg tcacgagcga tccgcaccgc atacaaaact
     3541 gcgccggatg cgcggtttat cattcatggc ggtgatctgg tcaataatgc tcgtgactat
     3601 ccggctttaa atgaatggtt cacagccgcc ggctggatca atgccacggt tccgattatg
     3661 ccagcaatcg gtaaccacga atattatgat ggaaattggg gcgagcaatt ttttatgccg
     3721 tactggcggc cgcaattcac tttaccttta aacggaatcg aggaacttcc tgagactaat
     3781 tactatttcg attttcaggg cttgcgtgtg attgtactta attccaattt tgagttagag
     3841 aaacaggcgc aatggttgga agaaattttg aaaaataacc ctaataaatg gacggttgta
     3901 gccttccatc atcccattta ttgcggagct gaaggtcgtg attatcagga acttcgtgat
     3961 acctggaatc caattttcaa aaaatataag gtagatttaa ttcttaccgg tcatgaccac
     4021 acttatgcac gcggtaataa tccagagcgt ttgactattc gtcctaatga tccaacagga
     4081 ccggtttatg ttgtttcagt cagcggccca aaattatata atattaataa aatccgctgg
     4141 atggatcgtg cggcagaaaa tactcaacta tatcaaatta taaccattaa tgataatatt
     4201 ctcgattata aagcctatac cgttactgaa gaactttatg atgcttttca aatcatagag
     4261 aattctaaag gtcacaaagt cttttttgat ttacaaccaa agaattttcc agagagaatg
     4321 tataaaaata cattaaatgc accaaataat taggattgta taaatgaaat acgacaaaga
     4381 caaagtcgat aatatgattt tagctctttt atttcttact agttcatctg atcaatatgg
     4441 tactaaggca tggaaaggat ttgactttga ggctttggat cgtttatatc aaaaaggttt
     4501 aattagtgac cctaaagata aatcagcaac tttatcatta agtgaaaaag gtgcaaattt
     4561 atctaaagaa ttgttcttca agtatttcgt tattaaagat tgaaatattt ttcaataatt
     4621 aattgatatt cagagaattg ttattattaa taatagcatt ttataagaac tcagtccgag
     4681 aatttaattc attaatagta taatagaagg taggaaggga aaatcaattt tatcctatat
     4741 ataacttctt taaatttatc tgcaactata cataatatat cttatgcgta tattaatttc
     4801 agggcatttt tagacatttg taagtatcat tcaattcatt ttgaattatg ccactgttga
     4861 ttaaattccc agttatatga tatgtataaa aaaattattt tgttaatgtt aatttttgta
     4921 atcaatgcca gcggtgatga ttatgaatat tatttctaca atcctgacct tgattacggc
     4981 tctgaatcaa tttttaaccc aattagtttg ttcatcaacg gttcgtttga tattttgcga
     5041 aatggtgctc acaataaaaa tatttttgac cagccatata aaaccggttt taataatgtt
     5101 attgataatg ttttgcatcc tttagataat attagaaatt atggtgttaa aaattttctg
     5161 aatcgtgaaa tatttaacct tgaagtaaat aaaaatgaat cccaattttt acccaatatt
     5221 ggtttacatt tgattggcaa tggtatgcaa tatcgcaaac tagctgaatg gtatgattac
     5281 catgaatatc caacacccaa tctgttatca ataataacta caatatcata tcaattggta
     5341 aatgaaatcg tagaaaatgg ttcatattca ggcactaacg tagatccgat ttcagatatg
     5401 ttaatattca atccattagg aatattactt ttcagtacaa aagctggaca agagtttttc
     5461 tcaacaaaaa tgccaattta cgattggtca atgaatccct attttaatcc aaataactat
     5521 tatttagaga atgccggcca gcaatatgcc acaaagatta agttaaaaac gactgataaa
     5581 aatgcgcttt ttggctattg gggtgttgtc tctacttttg gaattagcca taccataaat
     5641 gaaactgatg cgatatcatt ctctgccggt gctgttgtag ctaaattaac gcctaatgaa
     5701 gaatatgata gtcgttttat tattccaaac cttgattacg ctgtagcttt tttctatgat
     5761 agaaataatt cattactcgg atctttaact ttatttggtc ctaagtttta taatcttcgt
     5821 ctggaaatat tcccgggcat aattggattt aaaaattata aaccaggttt ttttatagga
     5881 attggcgaga tggataattt tcagtttgga atctcatttc cacccattcc tattggaata
     5941 attggagaaa ttagcaacta aaatggacat actatgaaaa tattattaac aggcggtgct
     6001 gggtatatcg gaagccatac attggtcgaa ttgttgaatc aaaaccatga gataacggtt
     6061 attgataatc tttccaatag caaaatagag tctataaaaa gagttaagac tataactggt
     6121 aaagaattga tattcaataa gattgaccta tgtaatatca ttgaacttga aaaagtattt
     6181 tcttctaatc aattcgaagc agtaatccat tttgccggtc tgaaagccgt tggtgaatcc
     6241 gtccaaaagc ctctgcttta ctattataat aacattacgg gaacgattaa cctctgccag
     6301 atgatgatta aatacgatgt caagaatatc gtgttcagtt cttccgctac cgtgtacggc
     6361 aatccgacat cagttccaat taccgaagat ttcccgatca agcccgcgaa tccttatggt
     6421 aataccaaat atattattga aacgattcta aaagacattc aaatcgctaa tccggattgg
     6481 aatgttatca tattacggta tttcaatcca gttggtgctc atgaatccgg tttaatcggc
     6541 gaagatccta atggcattcc taataattta atgccttata tttctcaagt cgcggtagga
     6601 cgtctcccct atttaaaggt ttatggaaat gactacccca ccaaagatgg caccggtatc
     6661 cgagattata ttcacgttgt tgatttagcc caaggacatt taaaagcatt ggataaatta
     6721 tcgacaaaac caggttattg catttacaat ttaggtactg gacagggata tagtgtgctc
     6781 gatgtaatta cgacttttga gaaatggtct caacgaaaaa ttccttataa aatcgttggc
     6841 agacgcccgg gagacattgc cgaatgttat gccgatccga gcaaagccaa tcgcgaactt
     6901 gcctggaaag cagaacgaaa cttagatgac atgtgtcgag atacctggaa ctggcagaag
     6961 aaaaatccga acggctattc agactaattt tctaatttta ctaaaatatt tgtggattct
     7021 attaaatatc cataaaacct ggaatggttc tggtataggc tttctgcagt tcagttaaag
     7081 gtaaatcaat aatattatta attgtcaaac gaccgttgtc tttaacgcgt ccgattgtca
     7141 cacttgggat gatattttta gaggcaattc gttctatatc aagtaaatat ttttcactga
     7201 tggatatgag gataactgat tgactttcac caaaaaacag ttcatcattt ctaagtttgc
     7261 gcgaaatata gactgaggcg ccaagcggtt tttcaggatt ggagatgcaa gcttctgcca
     7321 gagccacagc caaaccgccg tccgatatat ctattgctga attcacaatc cccttttgaa
     7381 ttgcttccaa ggtagcatta aagattcttt tttcaaacgt taaatctata tcaggagcat
     7441 caccagcgat aatgttatga accactttta gatattcact tccccccagt tcgcctttta
     7501 aggctcctaa taaaatgata aagtcgccgt cgtttttaaa ccagggggta gtcacgtttt
     7561 taagatcttc aatcagacca accataccaa ttactggcgt cgggaaaatg gctcctttag
     7621 gagattcgtt ataaaaactg acgttgccgc cagttaccgg cgtatcgaaa acacgacagg
     7681 catcaccgat gccctgcacc gccattttaa attgataata aatttctggg tcgtatggat
     7741 taccaaaatt tagacaattg gtaatcgcaa tcggtcgcgc accggaacag acaacatttc
     7801 gagccgattc ggcgacggca atcgcaccac ctttatacgg gttgagataa gtataacgac
     7861 cattcccatc agttttgata gccagtgctt tacgagttcc tttaatacga ataattgcgg
     7921 ctgaaccacc gggcttgaca actgtatttg atcttacgga ataatcatac tgccgataaa
     7981 cccaggattt atccgcgata ttcggcgacg ataatatttg cagaagcacc ttattgtagt
     8041 ctttaggctc gggtattttt ttcaaattac gcccctgcac ttttttcaaa taagctggtt
     8101 ctttggtttc tcgctcataa accggtgccc cgccacctaa taccagatgc cacgcgggca
     8161 gagaagcgac ctttttatta ttcataaaaa tatccaaatt accagaatta acaaccttac
     8221 cgattttgac acagtttaaa tcccattttt caaaaatcgc cttagtctct tcttcaaaac
     8281 cttttcgaat gacaactaac atccgttctt gcgattcaga caacataatt tcataggggc
     8341 tcatacccgg ttcacggaga ggaacagaat caaggttaat aatcattccg gatttgccgc
     8401 gcactgccat ttcggaagtc gagcaagcaa ttccagccgc acctaaatct tgaatgccga
     8461 ttaaaaaatc ctgcttggca agttcgaggg tagcctccag caatagtttt tcggtaaagg
     8521 gatctccaac ctgaactgaa gaacgccgat tctcagattc ttcagttaat tcaacggatg
     8581 cgaaagttgc cccatggatg ccatctcttc ccgtagaaga gccgacgatc attacggtat
     8641 ttccttctcc tttggcaaca gcactaacga catttttatt tttgaccaag ccaatagtca
     8701 taacattaac caacggattt tctgtatagc aatcctcgaa ataaatttcg ccagctacag
     8761 tcggcacacc gacgcaattg ccataatggg cgattccttt taccacgtta tcgaagagat
     8821 agcggttgcg tggtgcctcc aatggcccaa aacgtaacga atcgagtgaa gcgatcggtc
     8881 gagcgcccat ggtaaaaata tcccgtaaaa tccctcctac cccagtcgcc gctccctgaa
     8941 atggctcaac tgctgacgga tgattgtgac tctcaatttt aaaacaaatc gccagcccat
     9001 caccaatatc aactaaacca gcattttctt caccagcgtt aactaaaagg tgctttccac
     9061 ttctgggcag agttttaatc aaagctatcg aatttttata gctacaatgt tcactccaca
     9121 tcactgaaaa aatgcccagt tcagtgtagg taggcgtccg tcctaaaatc tttaatatct
     9181 tattccactc ttcctcggtt aaaccgtgtt gaatagcagt ttgcaggtta acttccggtt
     9241 caatctggct agccattgat tactccagta atgtgttaat tgtcaaagaa gtatcggata
     9301 tgtaattcga ctccaataat cactcattgt ctcgcgtcga gtaatttcaa tcgccttgcc
     9361 attaattata gctacttcgg ctggacgaag tcggtgatta taggtcattg acatagtact
     9421 tccgtaagca ccagcctgag taaaaattag caaatcgcct tcttctaact taggtaaagg
     9481 ccgccctacg gtgaaaatat ctgtattttc acaaatctga ccacaaagat taatcggata
     9541 gagttctttt tcatcttctt tgccatcgac aataaccgga tgctgggctt tataaagtgc
     9601 tgaacgaatc aaagtattga atccggcatc cagtccagca aatttctgat agctctcctt
     9661 aattcccaga actcgtgtca ccaagtaacc ggcgtcgccg acgaggtaac gaccgggttc
     9721 taaatgtaaa gccggtttgc caattgataa actttcagac ttggctttaa gcaccttgag
     9781 gcattctctt ccaacccatt gaatatcaag ttcatgctcg tctccataat aaggtattcc
     9841 gaatccgcca cccatatcaa cgtattcaaa agttatcccg agtttttggt ggatttggcc
     9901 taagatgttc agtaataatt ccagcattcg cggaaagtgt tcttcgtcga gaatgccgga
     9961 accggtcatc atctgagccc cataccgttt aatccctacc tgacgggctt tttcataagc
    10021 gagccacgct ttttcgtggg gcacaccgaa tttagccttc tcaccaccgg tggtaatttg
    10081 ttcgtactta cctctcccct tccctggatt aatccgaaaa cttattacct caggacaatg
    10141 aattcgtaac aaacgatcca gcgaggtaat atcatccaaa atgatattta ctcccgcttc
    10201 gacggcaaca ttgaaatctt ccatgctttc gtaattaccg gcaaagatta tgtccgatgg
    10261 ttttactcca gttttcaaag caatttcgat ttcacctggg ctaacgcagt cacagccata
    10321 accacaatct ttcaacagtt gtacaattgc gggatgcgaa ttcgctttca cagcataaaa
    10381 aaaggctgtt tcaatttcat cagaatcaaa agctctccgc aaacgctgag cattgtcttt
    10441 aatacgatca aaatcataaa cataaagcgg agtcccaaat tcatcagcta aatcgctgac
    10501 attcaatcca ttaatcttca tttaaattcc tattttatag atattgtttt attttttgag
    10561 cgatttttgg cccaatcccc ttaacattca tcaagtcttc catactggcg gattttatat
    10621 tggcgatcgt tttaaaatgt tgcagtaaag cctttttggt attctggctc aagcccggga
    10681 tttcatcgag tttggtagaa attccgcctt ttccacgtat ttttcgatga taggtgatgg
    10741 caaagcgatg cgcttcattc cttactcgct gtaaaaggct gagtgccagg ctgttgcgtg
    10801 ctaataatat tggttcatca ttccccggta taaatatctc ttccagtctt tttgccagac
    10861 caattacagg aattgcggat aaattcaatt cctgtaatac agtaacagcg gcattcagtt
    10921 gaccttttcc accatctatt agaatcagat ccggtagagg cttttcctca cgaagaagac
    10981 gtgtgtatcg gcgccgaaca acttccgcta acatagcata atcattactt ccggttacag
    11041 tttttatttt gaatcgccgg tactcactgc ttttcgcggc gccgttgata aatgtaatca
    11101 tagaaccgac ggcaaattta ccgccaatat tagatatatc aaaagcctca atccggcgcg
    11161 gcaactcttt taaacctaat tcattctgaa gagccacaag cgatttcggc acgaattcgg
    11221 ttttttggct ctgtttcatt tgcaactcgc tgatttgcaa ttcggcattg cggatagcca
    11281 ttttcatcaa ccgggcttta tcgcctcgtt gcggctggag gatactgact cgggcacctt
    11341 tttgagacat aagccatttt tcaagcattt cttgttcttc cggtagatag tttagcaata
    11401 cttccgatgg gatactggtt gcattatcat aatagcgcag gagcaagtcg tgagtaattg
    11461 ccgggtaatc atagttttcg gttccttcca gtaaaaattt atcgcgaccg atcaactttc
    11521 cctgcctgac ccgaaatatg acggcacagc aattttcagc ttctttcaga acggcgatta
    11581 agtcgcgatc gagtccatca ttcaactcga ctgattgacg attggaataa ttcataagaa
    11641 tggaaatctt atcacgataa gccgcggctg tttcaaattg cagactcgcc gcggcagttt
    11701 tcattttttc ctcaaaatat tcaatagact cttcggttcg cccgtttaaa aacgcgatca
    11761 cctggttgat catacgatta tattcctcgg ccgaaactag cctctggcaa gggccctcgc
    11821 attttttaat atgataatcc aggcaaactt tgatgtgccc gttagcaatg gttttatcat
    11881 tcaaatggtg tttgcagctt cgaataggaa aaatgttttt gatgatttcg agagtatcac
    11941 gcaggagacg actatcggta tatggaccga agtatttcga gccgtctcgc acaacgctac
    12001 gagtcataaa aacttgcgga aaaggttcat tggtaatgcg aacgtaggga taagttttat
    12061 cgtcccgaaa aaagatgtta tactttgggt gatgcgtctt aattagagta ttttcgagaa
    12121 tcagagcttc gatctccgaa ttagtcagaa tatactcgac atcagcaata ttattaacca
    12181 atgcggcggt tttaggttct ttataacctc cgacaaaata agatcttact cgattacgaa
    12241 tgtttttggc ttttccaata taaataattt taccggctga attacggtaa atataacaac
    12301 ctggatcagt cggggcagtt cggattttct cgagaagttt ttcggggaat tgtcgttcgg
    12361 ccatcagtta atatccacag ccgtaagcca aaatccactc cagctatgta aacttttagt
    12421 gaatagaaat aacaacatct gcgtcatcgg cctttattaa ctgatttgtc ggtcgcactt
    12481 tgttcagccg gacaaattcc atttttatca actaatctat caagaagttt gcaatgaatt
    12541 gcaataaaag tctgacaggt tgctgagcca tcagacagtt tatccggaaa ttccgcatat
    12601 tcacaagata aatcacaata cttcatttat taccctgcca attctcgccc aaatttaaaa
    12661 tgaaaaacgg gtagaatcaa atcatcgtat agctctaccc gtttttactt ggtattttta
    12721 ttttactaaa ttaatgtcaa aaccttcggg tcttccaatt ggctcggctt caactttccc
    12781 cttctttatc tttcgtttca aagcaatttg ttcaaagaaa agatatataa tgggtatcaa
    12841 aattaaggtg aataccgtgg caaaggttaa tccgcctatc actgcgatag ccatcggagc
    12901 ccataattct gcaccggtgc cgaggcctaa tgccagcggc aacattccca aaatagtggt
    12961 tagtgcggtc attaacacag gtcttaatcg tctcttactc cctgccagaa tagcgatcca
    13021 cagatgataa ttatgtttgt accttagttg atttatatag tcaactaata caattccatt
    13081 attgacgacg atacctacta atagtatgca tccaaccaat gccataacac tcatgggtgt
    13141 tccagttata aataacaacc agattgctcc aatcatcgcc agtggaatag taaaaatgat
    13201 gataaacgga tctagcaaag attcaaattg ggaagccatt accatataga cgagtatcac
    13261 agccgcagct agagcgatat agagatacaa aaaagcctcg cgcctttctt cctcggcgcc
    13321 gcctatttcc catctaaatt caggtgggaa atttatattt ctcaaaccag cagttatatc
    13381 tcttgtaaca ctttggatgt ctctaccgga aactgaacaa gatacactca ctcttctttc
    13441 ctgatcttct cggtttattg attcctcacc ggctccaggc aaaactctag caatattttt
    13501 taatggaaat tgagccccgg ttggagtagt aatatataga ttttcgatat cagcctgaga
    13561 ttttctgaac tgctcatcta attgaactat tacatcatat tcatctccac cttcacgaaa
    13621 tctggtgacc cgagttcctc tgatgctcgt accgactata ttagcaatct gataagtact
    13681 caaaccaaat gcagaggctt tatctctgtc aattattatc tgatactccg gtttaggaag
    13741 gttgtaactt ttagtaacat caacaactcc ttttatgtct ttcagcattt ccgctacctg
    13801 atcacctaaa tccctggcga ttaatctatc gtaaccgaaa atcttgatag tgataccacc
    13861 acttaacata gaggtagctt cttcaccacc agcaatggta atgttagcac ccggaatctt
    13921 ttgaaatctt tctcttaata tgtcttgaat ttcgaaggaa gatctctctc tttcgtctac
    13981 atcaggtaag cgaactgtaa tattaccaga attagaactt gccataattg cagtaacacc
    14041 ttcgccaact ccgaagttaa cctgaatatt ctcagcttcc ggcacctctt ctctgattat
    14101 cttctctatc tctctaaatg tcttgtccgt cgaggctaaa gatgccccag tttctctttc
    14161 gacacttatt ctaatattgg atctgtccgc ctctggtaaa aattcaccac ccagaaatgg
    14221 aaacattaaa atagttatca caaaaattac gatcaatcca attaaaaata ccttcttgtg
    14281 accgagaaaa taatctaata ttcttacata tctatcatga agtccttgaa gaaaattatc
    14341 aattacggaa tccattcttc taaggacctt tgattttgac ttctttttct cgccctttaa
    14401 ccattgcgta gataataatg ggattaaagt taaagcaact aacaaggaaa tagtcaaaga
    14461 tgatacaata acaataacta aatctttaaa aagaatacca gaaatccctg gtagaaaaag
    14521 aaccggaacg aaaattgcta tagttgttaa agttgaagcc gtgatcgcca ttgaaacttc
    14581 atttgctcct tcatcggctg cttgtctaat atctagcccc ttttccttaa gcctaaatat
    14641 attttcaaga acgactatcg aattatctac cagcataccg atagcaagag ccaagcccgc
    14701 catagttatg acgttaaagg tcatattgag caaatacatt gcaaaaaagg taactatgat
    14761 agaaaccgga attgagatcg cagttatcaa agaacttctg atatacctaa gaaagaataa
    14821 tagaaccaga aaaaccagaa taaatgcttg gactccggta ctcaccaaat tgtttagtga
    14881 tttcgtaata aattctgaag aatcaaagac aatctcaagt tttacatctt taccgaccaa
    14941 ttccattatt ttcggcaatt cttttgctac tgctcttgcc gtctgcaccg tattggcatc
    15001 tgattgtttt tggataaaaa gcatcactgc gtcttcgccg ttattcctca caatctggtt
    15061 ttgttcttta taaccatcaa tgacattcgc aacattcttt aaataaatag gtgtgccgct
    15121 aacatttccg attactgtat tagctatttg gtcaacagtt ctatattccc cataagtccg
    15181 gacagaatat tcttgatatt catcatcaac aacaccggcc ggaatctgga cgttttccat
    15241 ttgtagcttt tgaacaattt gttgggctgg tattccataa gccgcaactt tttgcggatc
    15301 aagcaggact ttaatttgtc gctctaaccc acctccagat gcggccgaag caacgccttt
    15361 tagtctctca attcgcggcc taacctcgtc atctatcact cttcttaatt ccgccatacc
    15421 cagttgaccc gatctggctg aaataagcat aacgggagtc attgacggat caaaactgat
    15481 ggttatggga ttcgatgcat ctggagggag ataatctcga attaaatcta tattttttcg
    15541 gacatcaatc tccgattgat caatatcggc atcccaatcg aattgcaata ttaccagtga
    15601 attaccaaca ctcgacatcg aaatgatttt atcaacgttt tcaactgcta cgagtgtttg
    15661 ctccagtgga cgagtgataa cattctcgat atcttcaggg cctacgccgg tatattgggt
    15721 cattaccccg actactggta tggtgatatt cggatataaa tctaatttta attgagtaag
    15781 tccgaaaata ccgaacccaa ttacaattag atagatcatt gtaaaggtaa tacctcgttt
    15841 aattgcaagg ttggataaac tcatatcgct ttccctttct caataatctc aacttttgtc
    15901 gaatctgata gattatgctg tcccattgaa acaattaaat ctccgtcatt taacccttgt
    15961 aatacttcta ctattcttcc gttttcaatg cctgtttcaa tttttacttc tcttgcaatt
    16021 cccgattcta caacaaaaac atatttgttt attatagttt tagttgaact aatgtcgcca
    16081 ccgatatatt ccagcgaagt tctttcaata atagaatacc tcgggataat taaggcattt
    16141 ttatgaacat cggtaatgac atcaactctg gcgaacattc ccggtctgag tttatgttca
    16201 gggttggaaa taattatttc cgctggtgag gttttcgtca taagattcaa cgtggggcta
    16261 attttagaaa tttccccgac aaaaactgta tccgggtagg tatcaacatt aatgtaagct
    16321 ttttgtccga ttttcacttt attgaattga gattcaatga cgtctatggt tatttttaca
    16381 tcatccattt caacaacggt gaaaaccggt atttgaggcg ccgcctgatc accaacatcc
    16441 aataagcgcg aggaaacaat tccggcgatc ggggctttga tttgggtatc ctctaattgt
    16501 tttttagctg tagacacagc ggcttccagt tgttttatgt tcgctcgggc ggcatctcgc
    16561 tgagtcgtga ttgcgtcgta ctgtgattta ctaacggcat tatcatcata tagtttttta
    16621 attctattcc actctgtttc gatatttctg agttgcgctc tggcggcttc cagaccagcg
    16681 ttcgcttgct ttactgcctg ttctatttta tcactttcaa ccacggctaa aacgtcgccc
    16741 ttattaaccc aatctcctac atctacattt atttttaata tcttattagg gattaaggaa
    16801 tagattttga tttccctgaa acctttaata tttcccagga aattgatttt ttcttcgata
    16861 gttcctcttt cagcggtaac gacgttgacc ggaacaatgt tctctgtcat ttgttcgtcg
    16921 gtttgggttt tactacagga attaacatag tagattatcc cacaaacaag aaaaagattc
    16981 agaatatttt ttgataactt catttttatc tccgtgatta aagttgattg atggcttttt
    17041 ttaaatttgc taaagctaca ttgtattcgt ataatgattt ttgatagttc atcttagcct
    17101 gattgagagc aacgttggca tttaatacat ccaattgcgt gctcgcgccc tcggaataca
    17161 ttaattctgc taatcgcaag gcttctttag cctgctcaat ggttttctct tgagtttgga
    17221 cattttcatt agcctctttc attttgaaat aagccgcctt aacttccagt tttattccat
    17281 caatagcaga ttcagcctga tagtcaactt ccttaacggc tattttcgcc tgttgaactt
    17341 tggctttggt cgcaaaaccg gaaaaaatcg gaatactcaa actgattgat gaattgaacg
    17401 atttcaccag atcatccgta gtaaaatcaa aatcatccct gaatgcctga tattgatacg
    17461 ctgtaccaaa aacaagcgat ggagaatatt tagccttagc taaattaacc tgcttttcgg
    17521 ctaatttttt tctatcttcc agcataatta cttcagaccg ttctgccagg gcgattgtga
    17581 tcaattcttc cagcgtcata ttgatcaaat ccgattcttt atatgacaat ttttctaagt
    17641 agtttatgtt gatatcttcg cctataccta ggataagcct caaatttgat tcggctaatt
    17701 tcttagcatt gttggccgac actaccatcg gtttaaaatt cgctacttga acttccgctc
    17761 ttaaaacatc aaaacgcgaa gctttgccga catcaaaaaa cttctgcacc tgcgatagat
    17821 tttgttctgc agaatgtaaa gcctcttccg ctacttcact ggctgagttg gcaaataaaa
    17881 caccataata ggcgttagtt acatcactca atattttctg ttcggtagct ttgagctgcg
    17941 actcggtgat tcttacgcca atcttcgaca tctgatatga attcagaatt aaaccaccgg
    18001 taaaaactgg ttgagataaa tttaaaccag ctacaaaagt attctctctt tcaaaagaca
    18061 ttgtttcttg aggtgtacca ccaagaagcg taatcaaatc tttgaactct tgcggtgttg
    18121 gcattccacc aacttcccag gcatgcttaa attgggagaa agcactaatg gaaggcaaca
    18181 tgctacccct gacttcgttt acactggctc tggattttaa cacagcctcg cgggctaatt
    18241 ttaaagaagg attacgctgt aaagctattt cccgggcagt ctcaatgtca aggttaagtt
    18301 cttccgcaaa tagactggcg atagtaatta aaaatactac gccaatcttt aatttaaatt
    18361 tcatcaaata ctcctttata tattaataaa ttagtttagt gattacgtgc accattgaga
    18421 agtgtattaa cgattacatt agcattttgt tcatttaaat tttcagtccc atttttaata
    18481 tgatccataa taaactggtt aattcctcct atataacaat ataaaaaggc attgacatcc
    18541 aaatcggatt tgagataacc tttttccttc ccttcggtga tgatatgaat aagcatttct
    18601 ttattctttt gcatttgctt ataaaattca actttggttt cattgggaat attacggatg
    18661 ggtgcattaa ttaagaattt ggttatttca ggattctgct ttaatctggt aacttgcata
    18721 ataacaatat catataattt attgatcggg tccttatcag attggtatat ttctgttaaa
    18781 aattgaactg aatagttggc aatagcattg atgatttcag caaatagagt atttttatcc
    18841 tggaaatgat aataaagggc aggtttggtt aattcgcata attcggcaat ttcgcgcatt
    18901 gaggttgctt cgaacccttt atcagcgaat aattttgcgg cggtttttat aattttatct
    18961 cttgtaatat tttcacattt cattttgtca atccttactt accggtaagt aagt
//
LOCUS       DAOOMU010000039        18918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_2985_length_18918_cov_20.870911, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000039 DAOOMU010000000
VERSION     DAOOMU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 18918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18918
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2985_length_18918_cov_20.870911"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLI55_05265"
     CDS             complement(<1..371)
                     /locus_tag="PLI55_05265"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HPB00276.1"
                     /translation="MKFGVVVFPGSNCDHDAYHVIKHVLGARVEYIWHKDTTLRLFDV
                     IILPGGFSYGDYLRAGAIARFSAIMPAIIQFAARGGKVIGICNGFQVLLEAGLLPGAL
                     IPNDHMRFRCQEVYLKVINKAT"
     gene            complement(371..625)
                     /gene="purS"
                     /locus_tag="PLI55_05270"
     CDS             complement(371..625)
                     /gene="purS"
                     /locus_tag="PLI55_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HPB00277.1"
                     /translation="MWLAKVVVSYKEGILDPEAKTIQKALKTLGYQNIEELSVGKYFE
                     IKLNSNLERSDVEKLVREICDRVLSNPVIQRYNYEIVETE"
     gene            complement(626..1336)
                     /gene="pssA"
                     /locus_tag="PLI55_05275"
     CDS             complement(626..1336)
                     /gene="pssA"
                     /locus_tag="PLI55_05275"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPB00278.1"
                     /translation="MLPSILTILNLFAGFLALLQISQGHYIAAVELMITALIFDGLDG
                     KIARFTHQTSDFGIEFDSLCDMVSFCLVPAVLIHELFVSELGFIGGLISFFPLLFGGV
                     RLARFNLQAGAGKKSYFIGMPTPTSALTIGSYIWFSYKVLGNYGNPKIILPLVVVLSF
                     MMVSNIRFSVNLKLGFRRGLPTAFKTGMIILMTIGMVIFNGYVIFPLVMVYIITHLLQ
                     WLIGYDEPRIHLARRKAH"
     gene            complement(1351..1986)
                     /locus_tag="PLI55_05280"
     CDS             complement(1351..1986)
                     /locus_tag="PLI55_05280"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPB00279.1"
                     /translation="MIAKESLRLIIPMLIVAVLAIFLAKYFDLGIMMVLFWVTLVSII
                     FTLYFFRDPERITPQNPAFLIAPADGKVVGISEVNDPFVGGGIKIAIFMSPFNVHINR
                     APYSGIIRQIEHKCGKFHSAFKPEAAIENEQCRITMEKDNLKLAVTQIAGFCARRIIS
                     RVKVSQNLEKGERFGLIMFGSRVELIIPKGLKINICPGAKVKAGETVIGEI"
     gene            complement(1983..2696)
                     /locus_tag="PLI55_05285"
     CDS             complement(1983..2696)
                     /locus_tag="PLI55_05285"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPB00280.1"
                     /translation="MIKNEKLYEGKAKIVYSTDDPELVIQYFKDDATAFDGIKKGQIS
                     GKGVANNLISCQLFSLLENNGIATHFIKKLSDNEMLVRRVKIIPVELVVRNVAAGSLC
                     KRYGVPEGQVFSEPIVECYYKNDAFHDPAMNDDHIIRFNLANADELSAIKATALRINQ
                     ILKEYFDSIGIILVDFKLEFGRYNDQILLADEISPDTCRFWEKGTNRKLDKDRFRFDL
                     GEVEEAYTEMLKRITGIQK"
     gene            complement(2683..3993)
                     /gene="purB"
                     /locus_tag="PLI55_05290"
     CDS             complement(2683..3993)
                     /gene="purB"
                     /locus_tag="PLI55_05290"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPB00281.1"
                     /translation="MIERYTRPAMGRIWSEENKLATWWRVELEVCRVQNERGIIPDEA
                     FQEIESRAKFSVKRIQEIEEEVKHDVIAFLTNISENVGPAARFIHKGMTSSDLLDTAL
                     ALQLSEAGQLILADLNQLEKILTEKSIEYKDCLMIGRTHGVHAEPITFGFKLIIFREE
                     IRRHIKRFELALDDIRVGKISGAVGTYQHIEPEVEERVCHNLRLEPAPVTNQIIQRDR
                     HAFFIATLALIGTTLEKIATEIRHLQRTEVLEVEEFFSEGQKGSSAMPHKRNPVLSER
                     ICGMARLLRGYAVTAMENIPLWHERDISHSSNERVILPDACICTDFILNEMSKVLTNL
                     LVYPENMRRNLEGMHGLIFSQEIMLAIIEKGASREEAYTIVQNNAMKVWQSGSDFKEL
                     LLKDENVLKWLNPAEIESYFNYEKVLTRINRIFERISEGNYDKE"
     gene            complement(3997..5097)
                     /locus_tag="PLI55_05295"
     CDS             complement(3997..5097)
                     /locus_tag="PLI55_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPB00282.1"
                     /translation="MKKINSVLISSLFIGVSLLLAVPQEQQEISNSRRNAITAAIEKV
                     SPAVAGINVTAIEEYVASPFFNDPLWSLLFPEQIYRRRVKSIGSGVVISSDGYIVTNT
                     HVVEGAEEIVVTLMGGKEYKAKLVGIDNISDIALLKIDGKSFTYAKLGSSEDIMIGEW
                     VIALGNPFGLFSVNYKPTATIGIVSGTDLDFGPQQSGRLYQDMIQTDAAINTGNSGGP
                     LVNALGEVIGINTFIFTGGQFSEGSIGIGFAIPVDRVKAVVANLKQYGKDSRTFKIGW
                     ELQNVDSFLARYLNLPSNTGVIVTRVEKGTPAERSGLKVGDVIISINGKKVARAEEIY
                     AYIQENLLRQGDKIKVRFLRGNREYETQLVLE"
     gene            complement(5094..5738)
                     /gene="fsa"
                     /locus_tag="PLI55_05300"
     CDS             complement(5094..5738)
                     /gene="fsa"
                     /locus_tag="PLI55_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HPB00283.1"
                     /translation="MKLFIDTANLEEIRKAQSWGLLDGVTTNPTLLSKEKGNFKEILL
                     KICETVNGPVSGEVVSLDAEGMVREAEDLAALHKNMVIKVPITLEGLKAIKILSEKGI
                     RTNTTLIFSPLQAILAAKAGATFISPFVGRLDDIHQVGMELVQQIKTIYDNYGIRTEI
                     IVASIRNPLHIVEAALIGADICTIPFNVMEKMVKHPLTDKGITQFLEDWKKVEK"
     gene            complement(5741..6487)
                     /gene="truA"
                     /locus_tag="PLI55_05305"
     CDS             complement(5741..6487)
                     /gene="truA"
                     /locus_tag="PLI55_05305"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPB00284.1"
                     /translation="MGRRFKIIMQYDGSNYCGWQRQRYQPSVQAKIENALQPLNQNQP
                     VTVIAAGRTDSGVHARRQTAHFDLVTDLSPETIRKALNATLPDDIYIDYCQEVGKEFH
                     ARFTAINRTYHYQVVVNPNVFLRKYVWLVNFRFDRKLLQECAELVKGEHNFTALCRAA
                     TETENKMATIYESRWEEQDELLVYIITANRFLYAMVRMLVGSMMEVARGKYSIGAFKN
                     LLGKNDTKMQVYTAPAQGLFLWNVKYQEDN"
     gene            complement(6487..7233)
                     /locus_tag="PLI55_05310"
     CDS             complement(6487..7233)
                     /locus_tag="PLI55_05310"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPB00285.1"
                     /translation="MKNLDPRVKILWLIIAVIGIFRLKVWYAQLGVAIILGTIITFRI
                     LYAKENFKVLRILLVFLPLTFLIHILFGTNLLATIFRSAPRDSWSVLLFTPLTFTLRI
                     GNLIALMALATHWLKALEVLDALYHLLKPLRKIGLPVDDIFQIIFIAVRFIPLIQEEY
                     QRLDDSWRIFMNRDSGGLFGQIERLRTILVPLMVVSFRRAEVLAEAMTVRGYNSQVKR
                     SYIGRLHWQTPDWIGLAAGCGSLVLMVINI"
     gene            complement(7235..7921)
                     /locus_tag="PLI55_05315"
     CDS             complement(7235..7921)
                     /locus_tag="PLI55_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00286.1"
                     /translation="MKHLFCPSLLGLLIIFILAGSGTAQIDSTLVAQPDSLPQLTQSQ
                     ARLLLLKSAVLPGWGERSLNYVERSHYFNASEIALWVTYFAFYWYGNAVAADMEAYAA
                     THAGVNPDGKDQYYFTDIGNYENIYAYNEQKLRYRAINLLYPVTPEYYWAWDSESSRR
                     HFDKLRVKSATALRNATFAIGGLVANRLFSMLDVIVLTRNRLEMPDIDIESNWQPYQD
                     GMSFMLSLKF"
     gene            complement(7918..9468)
                     /gene="lnt"
                     /locus_tag="PLI55_05320"
     CDS             complement(7918..9468)
                     /gene="lnt"
                     /locus_tag="PLI55_05320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPB00287.1"
                     /translation="MKVKINLWWLILISGVLSGLAFPPLPLGFLAFFALVPLIKAFCD
                     DEFHNGFEKGLVYGLVLNLAIMYWLALNKGTTWYWATLSMVAAVIFLAMNYAIIGLSV
                     GFIGRRLGRNVGIWSFPIIWTAIEYLRSFGSLGFTWNNLCYTQSHAIQLIQSAAVVGP
                     YGISGWIVVINVLIFRLLENLEDYRQVSRRMVIIIALFLIPAIYGMVVLQKPSDENDR
                     RLVNVGLIQPNIDPNAKWDQESFKKNMQLLHDLTDSVATQPLDLVVWPETATPTFLRR
                     NHYAVLTEIREHIERLEVNLLTGTPDLERAGEDYQYYNATFLLRPHSNRIEDYRKIHL
                     VPFGEYIPLSGYFPELSNLNLGQGNFQAGKEVKLFQIPLKVDQRPVSDTVLTFNSVIC
                     YESTFPDLVRQATRQGSELLVIVSNDAWFGNTSAPFLHAEIGRWRAIENRIPVVRSAN
                     TGISLILDQYGRNLQSLPFGKSGWLSAPVTQGNPQTIFVRYGNWPARLCVILALLLIG
                     YSIIRRKK"
     gene            complement(9520..9825)
                     /locus_tag="PLI55_05325"
     CDS             complement(9520..9825)
                     /locus_tag="PLI55_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPB00288.1"
                     /translation="MKVKTYTKKDVVRRTAEALDTKLVDTAEIVDGVFMVLREMLSES
                     DPRIRIEIRNFGVFEVKPTKAKPKARNPRTNEEVYVPARRKTHFKPGRLLKERMKKA"
     gene            complement(9844..12024)
                     /locus_tag="PLI55_05330"
     CDS             complement(9844..12024)
                     /locus_tag="PLI55_05330"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPB00289.1"
                     /translation="MATEILNKVLPRAKQRPQKFLTLISKLNQRAPLTKDEEDFLWEV
                     YSFARNSHRGQRRQSGDSFFEHPYHTALILADLHMDPITVAGGLLHDVIEDTEVTVKD
                     IAEKFGDDVAKLVEGVTKISGITFRNRQEEQADNFRKMLLSVAEDIRVLIIKLADRLH
                     NMQTLDSLPPIKQRRVAIETRDVYAPLAHRLGMFKLKSEMEDLVLKALDPDAYRFLEK
                     KVNEKRSEREKYIKVFTQPIIKALNEQGIKAKIVGRPKNFYSIYKKMKTQNKPFEEIF
                     DLLAVRIIVDTKENCYTVLGIVHDLFTPVMDRFKDYIANHKANFYQSIHTTVYGPSGR
                     MVEIQIRTTEMDEIAEEGIAAHWRYKEGRDKPDDIDKYVKWLRDLIEILQTESTGAKD
                     FMDTLKIDLFKDEIFVFTPMGDLIRLPKGATPVDFAYEVHTEIGTRCIGAKVDGKMVP
                     LNSELRSGQTIEIITSDSHRPSYAWLKFVTTAKAIGAIKRYLRKAQFEESINLGKEIL
                     EKENQRNKELRFLKTVQESFEELGFSELDKLYAAVGSGILTIPQIYAKLFPQKEEQLV
                     RQDLDELFIETARKSVKGIKVQGISNLMVSFAKCCNPIPGDQIIGFVSRGRGVIIHRS
                     DCSNIPSLLEDDERLLNVEWDIAGTQAFLVRLKIMAQERKKFLKDVTDIMASTDTNII
                     SVSGDVDESIIHLNLVIQVENLRHLNKILAKLQNVQGIISVERK"
     gene            complement(12024..13403)
                     /gene="dnaB"
                     /locus_tag="PLI55_05335"
     CDS             complement(12024..13403)
                     /gene="dnaB"
                     /locus_tag="PLI55_05335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPB00290.1"
                     /translation="MALNDGEIIRVPPHSDEAEVSVLGAMMLDKEAVSKALQYLDYTA
                     FYKEAHQHIFQAMTDLFNEGQPVDQVSVIDQLKRKKMLEQVGGAYYVTGLVEATPSAA
                     NVEYYANIVLDMAILRRLIVASNEIQTESYEARDSVQDILDTAEQKIFAISENRMKGG
                     FKNFVDVLNRTFEHIDSIHSRKGHTSGVPTGLLDLDELTSGFHPGELIILAGRPGMGK
                     TALALTIARNAAVEYDMPVAFFSLEMADYQLALRILCAEARLDSHLVRKGKLPKEQWQ
                     RLSMQTGRLANAKIYIDDSPVLTMMDIRAKARRAKAEHDIQLVIIDYLQLIKGHRAEN
                     RQQEITEISRGLKALSKEIGVPVIALSQLSRAVEGRADHRPQLSDLRESGSLEQDADV
                     VIFIYRKSFYDKREGSAASDEDERLAEIIVAKQRNGPTDTVEVIFIDKYTRFENKGAQ
                     REEIITTPF"
     gene            complement(13404..14081)
                     /locus_tag="PLI55_05340"
     CDS             complement(13404..14081)
                     /locus_tag="PLI55_05340"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPB00291.1"
                     /translation="MTRLYDDIKTFFKQQAELFPPEIYLISENGEESTPKTDAATQLA
                     ELEHICQNCRRCHLSATRTKVVFGTGNPHAKLLFIGEAPGVNEDLQGLPFVGRAGNLL
                     DKMLKAIGLTRAQVYIANVLKCRPPENRTPNVGEVEKCEPYLLKQIAIIQPELIICLG
                     LTSAKTLLRVEYTLEKMRGQIFQYHNVDLIVTYHPAALLRNENLKKYAWEDFKKIKNL
                     YLDKTGE"
     gene            complement(14082..15299)
                     /gene="coaBC"
                     /locus_tag="PLI55_05345"
     CDS             complement(14082..15299)
                     /gene="coaBC"
                     /locus_tag="PLI55_05345"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPB00292.1"
                     /translation="MNLVNRKIVLGLTGGISIYKSAGLLRRLVNDYKADVTVIMTDAA
                     QKFMSPLIFETFCHKPVLTDMFTGQKIETRHIDLATEADLILVSPATANIVAKTANGI
                     ADDLLSTIILVGGNKTVFALAMNDNMYANPITQENISRLRRLGYDIIEPEEGALACGS
                     TGRGRLADETTILTHIDRRLNGNKWLKDKKVIVTAGPTREYIDPIRFISNRSSGKMGY
                     ALAEIAFRESADVLLISGPTDLKNTVGVKTIRVETANEMRSAVLSEAAEADFLFMAAA
                     VEDLSPVQIATQKIKKESGISELKLKYSPDIIMDFRKHNRQACVVGFSVEIESAEHPG
                     AKARSLAKLNDKEMDFIVWNDPNVSGAAFGHETNSVTLLSKDGQEWVFPLASKRDIAD
                     SIISTVIKNYKKT"
     gene            complement(15283..15603)
                     /gene="rpoZ"
                     /locus_tag="PLI55_05350"
     CDS             complement(15283..15603)
                     /gene="rpoZ"
                     /locus_tag="PLI55_05350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPB00293.1"
                     /translation="MTETLPFSKLTRKSDDIFELVVVAAKRARQINALRMAQYPLPTM
                     VEEQEETFEETPPEITDEPAWDYLEKPTTLAINEILEGKINYRYMTPEKEKPLEEFDS
                     EFSE"
     gene            complement(15600..16169)
                     /gene="gmk"
                     /locus_tag="PLI55_05355"
     CDS             complement(15600..16169)
                     /gene="gmk"
                     /locus_tag="PLI55_05355"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPB00294.1"
                     /translation="MKPKGLLVAIAAPSGTGKTTVCHELMKRDDRFVFSVSCTTRPPR
                     PNERDGVDYHFISREQFLKNIHEGKMAEWQEIFGNYYGTLKDSIEQALRDGKILLLDL
                     DVKGTLNLKKNYPDQLISIFLLPPSDTELDRRLQKRGTDDSSSIAIRKARIPDELELS
                     EQFDFQIVNDNLEATVNKIINIIEEHLKK"
     gene            complement(16166..17110)
                     /locus_tag="PLI55_05360"
     CDS             complement(16166..17110)
                     /locus_tag="PLI55_05360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPB00295.1"
                     /translation="MAILNLTVWTGLTNFTIDLIGDLQLESMTGYSRTEYRSGGQVIS
                     VEIYSENNRFLDLTFNLPEPLEPYTEELKRLVASDLRRGRVRITIAITGLEKTPEVQL
                     NTAFLTKYYRALCDLARELQIEEPVTLQNLLTYPEIFITDSADTDVAELLTQVKDLLR
                     QAVAELIAMRRREGEFITRELHSHLQFILEKCSEIEKLSQQSRQEYFTKYQQSLAELC
                     QNCQLDENRLIQEAGILAKKLDITEECTRLRSHIQQFTLLLQSEEPVGKQMNFLAQEM
                     NREITTIGSKSESTAISHLVVSLKNELEKIREQIQNVI"
     gene            17133..18914
                     /locus_tag="PLI55_05365"
     CDS             17133..18914
                     /locus_tag="PLI55_05365"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HPB00296.1"
                     /translation="MQPIFIYEETKWNLSAPHELYSCNRLPPKQHPLGALINALLALL
                     FNMFNSWLHIERLAREYQQLIGGAICGMPFTYQKDHLHIPLEMAGEWHCLHFSVQAPL
                     PFFTLERTLPQPKHKVNLLRDVTAKAITDLVCRENDRQVLITLNSGIAYLLFHLYGIN
                     GNVYLLDSSGVSRDSFKKAKKPVDFELSNFRPANRLFPDYADFTSSLRQHSDQSLMTF
                     FTKSFQPILPKILVTEICYRTNLAPQYSIINLTDEQVERLAKTIAELSDEIVTSLPRI
                     YSGEPPIFSLIELQSLSNREFAEYDSVIAAQNHFIQDYLNLYRLKEKKKTLLRQTEFL
                     LSTTRRKLIKQQSELAALPTADNYREWADTLMATASTIPHHSNSVILPQLTDEARQIT
                     IPLEPKLNPIQNAERYYAKSRQIEKARAELIQKIAEAEQLIQRLENTQKQIAEADDFK
                     TLRSISSHWPAQPQSSIENIHQPFININLDGWEILIGKSARDNDELLQKFARPNDFWL
                     HAQGTSGSHIVVRNPGKVASLPRPILEKAAGLAAFHSKAKHSAVVPVIFTQCKYVTKP
                     RHSAPGAVTVKFEKTIMAEPLDPKKFL"
BASE COUNT         5312 a         4474 c         3804 g         5328 t
ORIGIN      
        1 gtggctttat tgattacttt aaggtaaacc tcctgacagc gaaatcgcat atgatcgtta
       61 ggaattaaag ccccgggcaa tagcccagct tccaacagta cttgaaaacc gttacatatc
      121 ccgatgactt ttccccctct ggcggcaaat tgaataatgg ctggcataat cgctgaaaaa
      181 cgggctatcg ctcccgcccg caaataatcc ccgtaagaaa aaccgcccgg caaaatgatt
      241 acatcgaaaa gtcgcaaagt agtatcttta tgccagatat actcaactct cgctcccaga
      301 acatgtttaa taacgtgata agcgtcatga tcgcaattcg agccggggaa caccactact
      361 ccgaatttta tcattccgtc tccactattt cgtaattata tctctgaatc actggattag
      421 aaagaacgcg gtcgcatatt tcccgaacca atttttcgac atcgcttcgc tcaaggttgg
      481 aattcagctt tatctcaaaa tatttaccga ctgacaattc ttcgatattc tgatatccga
      541 gagtttttag agccttttga atcgttttag cctccggatc gagaatgccc tctttgtaag
      601 acactaccac tttcgctaac cacatttaat gcgcctttct tctggctaaa tgaatacggg
      661 gttcatcata gccaattaac cattgcaaca gatgagtaat tatataaacc atcaccaacg
      721 gaaatataac atatccattg aaaattacca tcccgattgt cataagaatt atcatcccgg
      781 ttttaaacgc agttggcaag ccgcgacgaa aaccaagttt gagattaacg ctaaagcgaa
      841 tattacttac catcataaac gataatacta ccaccagtgg aaggattatc ttcggattac
      901 cataattgcc taatacttta taactaaacc agatataaga gcctatcgtc agagcgctgg
      961 tcggggttgg cattccaatg aaatagcttt tcttgcccgc accagcctgc agattaaaac
     1021 gcgccagccg gacgccgcca aataaaagcg gaaaaaagct gattaatccg ccaataaatc
     1081 ctaactcgga tacaaatagt tcgtggatta agactgctgg caccaaacaa aaagaaacca
     1141 tatcacaaag cgaatcgaat tcgatcccaa aatcagacgt ttgatgagtg aaacgggcaa
     1201 tttttccatc caaaccatca aaaatgagag cggtgatcat caattccacc gccgcaatat
     1261 aatgcccttg gcttatctgg agtaaagcca aaaatccggc aaacaggttc aaaatagtca
     1321 agatacttgg aagcaaattg cgtctcacct ttatatttct ccaatcacgg tttctccggc
     1381 tttgactttc gctcccggac aaatgttaat tttcagacct ttaggaataa ttaattctac
     1441 ccgcgaccca aacataatca aaccaaatcg ctcgcctttc tctaaattct ggctgacttt
     1501 cacccgcgaa ataatccgcc gcgcacaaaa accggctatc tgagtcacag caagtttgag
     1561 gttatctttc tccatcgtta tccggcattg ttcgttctca atcgccgctt ccggcttaaa
     1621 agccgagtga aatttaccac acttatgttc aatttgcctt ataatccctg aatagggtgc
     1681 gcggttaata tgcacattaa aaggcgacat aaaaatggca attttaatcc cgccgccgac
     1741 aaaagggtca tttacttcgc tgattcctac gacttttcca tccgcaggtg caattagaaa
     1801 tgctggattc tgaggagtga ttcgctccgg atcgcggaaa aaatagagcg taaagattat
     1861 actcactaat gtcacccaga aaagcaccat cataatcccc aaatcaaaat atttagccaa
     1921 aaaaatcgcc agaacagcaa caatcaacat cggtataatc aggcgcaagc tttctttggc
     1981 aatcattttt gaatccctgt aatccgcttt aacatctcgg tataggcttc ctccacctcg
     2041 cccaaatcaa accgaaagcg gtctttgtct agtttccggt tagtgccttt ttcccagaag
     2101 cggcaagtat ccggtgaaat ttcgtcagct agtagaatct ggtcattata acgaccaaat
     2161 tccagcttaa aatccaccag gattatcccg attgaatcaa aatattcttt cagaatttga
     2221 ttgattcgca gagcggtggc tttaatagcc gataattcat cggcattagc cagattaaaa
     2281 cggataatat gatcgtcatt cattgctgga tcatgaaagg catcgttttt ataataacat
     2341 tctacaattg gctcactgaa aacctgccct tccggtacac cataacgttt acaaagactg
     2401 ccagccgcca cgttacgtac tactaattcg acggggataa ttttaacccg ccgtactaac
     2461 atctcgttat ccgagagttt tttgataaaa tgagtagcaa ttccgttatt ttctaaaagg
     2521 ctaaatagct ggcaactgat tagattgttc gctactcctt taccgctaat ttgccctttt
     2581 ttaattccat cgaaagcggt ggcatcatcc ttgaaatact gaatgactaa ctccggatca
     2641 tcagtagaat agacgatctt ggctttccct tcgtataatt tttcattctt tatcataatt
     2701 accttcgctg atccgttcaa aaattctatt gattcgagtt aaaacttttt cgtaattaaa
     2761 ataagactca atttcggctg gatttaacca tttgagaaca ttctcatctt tcaaaagcaa
     2821 ttccttaaaa tcggagcccg attgccaaac cttcatcgca ttattctgga caatagtata
     2881 agcctcttca cgacttgcac ctttttcaat tattgccagc ataatctctt gtgaaaagat
     2941 taatccgtgc attccttcga gattgcggcg catattttca gggtaaacaa ggagattggt
     3001 cagtacttta ctcatttcat tgagaatgaa atcagtacaa atacaggcgt ctggcaggat
     3061 gactcgctca ttggaagaat gcgagatatc gcgttcatgc cagagcggaa tattttccat
     3121 agcggtcacg gcatagccgc gcaggagtcg agccattcca caaattcgct cggaaagcac
     3181 cggattacgc ttgtgcggca tagcagatga acccttctgc ccttctgaga aaaattcctc
     3241 aacctccaga acctctgtgc gttgtaagtg cctgatttcg gtagcaattt tttccagagt
     3301 cgttccgatc aatgccagag tagctatgaa aaaggcatgg cggtcacgct ggataatctg
     3361 gttagtgact ggagcgggtt ccagcctgag attgtggcag acgcgttctt caacttccgg
     3421 ctcaatgtgc tggtaggtac cgacggctcc ggaaattttc ccaactcgaa tatcatccag
     3481 tgccagctca aatcgcttaa tatggcgcct gatttcctcg cgaaagatta ttaatttaaa
     3541 cccaaaagta atcggctcag catggacacc gtgagtacgc ccgatcatta aacaatcttt
     3601 gtactcaata cttttttcag ttaaaatttt ctccaactgg ttcaggtctg cgagaatcaa
     3661 ttggcctgct tcgcttaatt gaagcgccag agcggtatcg agtaaatctg aagaagtcat
     3721 ccctttgtga atgaagcggg cggctggacc gacattttca ctgatattag tcaaaaaagc
     3781 gataacatcg tgtttgacct cttcctcaat ttcttgaata cgttttactg aaaattttgc
     3841 ccgcgattca atttcctgaa aggcttcgtc ggggataatc ccccgctcat tttgaacccg
     3901 acaaacttcc agttctactc tccaccaggt cgccaattta ttctcttccg accagattcg
     3961 tcccattgca ggtcgcgtat agcgttctat cattggctat tctaaaacta gctgggtttc
     4021 atattcacga ttaccacgta aaaatcggac tttaatctta tcgccttgcc ttagcaaatt
     4081 ttcctgaatg taagcatata tttcttccgc cctggcaacc tttttcccgt taatcgatat
     4141 aataacgtcg ccgactttca gcccagagcg ttcggctgga gtaccttttt ccactcgtgt
     4201 gacaattaca ccggtattcg acggtaaatt tagatagcgc gctaaaaaac tatccacatt
     4261 ctgaagctcc cagccaattt taaaagttcg gctgtcctta ccgtactgct taagattagc
     4321 tacaaccgcc tttacccggt caaccggaat agcaaagccg attccgatcg agccctcact
     4381 aaattgacca ccggtgaaaa taaatgtgtt gatgccaatg acttcgccca gcgcatttac
     4441 taatggtcca ccactgttgc cggtattaat cgccgcatcg gtttgaatca tgtcctggta
     4501 taggcgaccg ctctgttgag gcccaaaatc gaggtcggtt cccgaaacta tcccgatcgt
     4561 agctgtcggc ttataattaa cgctaaaaag accaaaggga ttgccgagtg caattaccca
     4621 ttcgccaatc attatgtctt ccgaagaacc cagtttggca tacgtaaaag acttgccatc
     4681 aatcttaagc aaagcgatat cggaaatatt atcaatcccg actaatttag ccttatattc
     4741 tttgccaccc atcaaagtca caacaatctc ttcggcacct tcgactacat gagtattggt
     4801 aacgatataa ccatcagaac tgattactac gcctgaaccg atgcttttta cccggcgacg
     4861 atatatctgc tcggggaaca gcaaactcca gagcgggtca ttgaaaaacg gagaggctac
     4921 atactcctca atcgccgtga cgttaatacc cgccacagcc gggctgactt tttcgatggc
     4981 cgcagttatg gcattgcggc gtgaattact gatttcttgt tgttcttgtg gaacagccag
     5041 taaaaggctt acgccgataa acaaactgct aattaacacc gaatttattt ttttcacttt
     5101 tcgacctttt tccaatcctc cagaaattgg gtgatgccct tatctgttag aggatgtttg
     5161 accatttttt ccataacatt gaaaggaatt gtacaaatat ccgccccgat tagagccgct
     5221 tccacaatat gcaacggatt gcgaatactg gctacaatga tttccgtcct gataccataa
     5281 ttgtcataaa tagtcttaat ctgctgaact aattccatcc cgacctgatg aatatcatcc
     5341 agacgcccga cgaagggact gataaaagtt gccccggctt tcgccgccag aattgcctgc
     5401 aaaggcgaaa aaatgagggt cgtattagta cgaattccct tctcggataa aattttgata
     5461 gcctttaatc cttccaaagt aatcggaacc ttgatgacca tattcttatg tagagcggct
     5521 aaatcctcag cttcgcgcac catgccttca gcatctaaac tgacaacttc gccactgacc
     5581 ggtccattga cggtctcgca tattttgaga agaatctctt taaaattgcc cttttccttg
     5641 gataacaaag tcggattagt ggtaacgccg tccaataatc cccaggactg agcttttcta
     5701 atttcttcga gattagcagt gtcaataaac agtttcattt ttaattgtcc tcctgatatt
     5761 taacattcca taaaaacagc ccctgcgccg gagctgtata aacttgcatt ttagtatcat
     5821 tttttccaag aagattcttg aaagcaccga tcgaatattt tcctctggca acttccatca
     5881 ttgaacccac cagcatcctg accattgcat ataaaaagcg attggcggtt atgatataaa
     5941 ctaatagttc gtcctgttct tcccagcgcg attcgtaaat cgtcgccatt ttattttccg
     6001 tctcggtcgc cgctcgacat agcgccgtga aattgtgttc gccttttacc aattcggcac
     6061 actcctgcaa caactttcga tcaaagcgaa aatttaccag ccagacatat ttgcgtaaaa
     6121 atacgttagg gttgacaacc acctggtagt gataggtacg attaatcgcg gtaaaacgag
     6181 cgtgaaattc cttccctacc tcctggcaat agtctatgta gatatcatcc ggcaaagttg
     6241 cattcaaggc ttttctgata gtttctggtg ataaatccgt caccaaatca aaatgggcgg
     6301 tctgacgcct ggcatgtaca ccgctatccg tacgaccagc ggcaatcacc gtcaccggct
     6361 gattctgatt cagaggttgc agggcatttt caattttcgc ctgcacggaa ggctgataac
     6421 gttgacgttg ccagccacaa taattgcttc cgtcatactg cataataatt ttaaagcgac
     6481 gtcccatcag atattaataa ccatcagaac caagctccca caaccagccg ccaatcctat
     6541 ccaatcaggt gtttgccaat gtaatcggcc gatgtaggag cgcttaactt gagaattata
     6601 accgcgtacc gtcattgcct cggctaatac ttccgcccgt cgaaagctaa ccaccattag
     6661 cggaaccaga atcgttcgca ggcgctcaat ttgaccaaat agaccacctg aatcgcggtt
     6721 cataaaaata cgccagctgt cgtcgagcct ttgatactct tcttgaatta gcggaataaa
     6781 gcgcactgcg ataaaaatta tctgaaaaat atcatctact ggcaaaccga tttttctcaa
     6841 gggctttagt aaatggtata gagcatccag tacctccagc gcctttaacc aatgagtcgc
     6901 gagagccatt aaagctatca aattaccgat cctcaatgta aaggtcagcg gcgtaaatag
     6961 taaaaccgac caggaatcac gaggagctga acggaaaata gtggctagca agttcgttcc
     7021 aaacaaaata tgaatcaaaa atgtcaacgg taagaatacc agcaaaattc tgaggacctt
     7081 gaaattttcc tttgcataga ggattctaaa agttatgata gttcccagta tgatagcgac
     7141 tccaagctgg gcataccaaa ctttgaggcg gaaaatacct attacggcta ttatcagcca
     7201 gagaattttt acccgaggat caaggttttt cattttagaa tttaagactc agcataaaag
     7261 acataccatc ctgatatggt tgccagttgg attcaatatc tatatccggc atttccaatc
     7321 ggttgcgcgt cagaactatt acatccagca tgctaaagag tcggttggct accaagccgc
     7381 cgatagcaaa agtcgcattc cgtaaagctg tagcgctttt cactcgtagt ttgtcaaagt
     7441 gacggcgaga agattcactg tcccacgccc agtaatattc gggagtcacc ggatagagca
     7501 aattaattgc ccggtagcgc aatttctgtt cattataggc gtagatattt tcataattgc
     7561 ctatgtcggt gaaataatat tgatctttgc cgtctggatt cactccggca tgcgtggcgg
     7621 cataggcttc catatcagcg gcaaccgcgt ttccatacca ataaaaagca aaataagtca
     7681 cccagagagc tatttcggag gcattaaaat aatgactacg ttcaacgtaa ttcaaactgc
     7741 gttcacccca gccgggaaga acagccgatt ttaataatag cagacgcgcc tgactttgag
     7801 tcaattgggg aagtgaatca ggttgagcga caagcgtaga atcaatctga gctgtaccgc
     7861 ttcctgcaag aataaaaatt attaataatc ccaagagact cgggcaaaat aaatgcttca
     7921 ttttttcctt cttattattg aatagccgat taacaacaat gccagtataa cacataagcg
     7981 cgctggccag ttcccatatc tcacaaaaat agtttgtgga tttccctgag taactggcgc
     8041 tgaaagccac cccgatttgc caaaaggtaa ggattgcaga ttcctgccat attggtctaa
     8101 aataagtgaa attccggtat tggcagaacg gacaaccgga atacgattct cgatagcccg
     8161 ccaacgccca atctcagcat ggagaaaggg tgccgaagta ttcccaaacc aggcatcgtt
     8221 ggaaacaatc accagcaatt ccgagccctg gcgggtagct tgacgaacca gatctgggaa
     8281 agttgattcg taacaaatca cggaattaaa agtcaaaacc gtatcggaga ccggcctttg
     8341 gtcaacttta aggggaatct ggaataactt aacctctttc ccagcctgaa aattgccttg
     8401 cccgagatta agattactca attccggaaa atagccagaa agcgggatat attcgccaaa
     8461 cgggacaaga tgaattttgc gataatcttc aatccggttg gaatgcggac ggagcagaaa
     8521 agtcgcattg taatactgat aatcttctcc cgcacgttcc agatccggag taccggttaa
     8581 tagattaact tccagtcgtt cgatatgctc tcttatttca gtcaaaacgg cataatgatt
     8641 ccgtcgcaaa aaagtcggcg tggcagtttc aggccaaacc accaggtcca gcggctgggt
     8701 cgccaccgaa tcggttaaat cgtgcaacaa ttgcatattt tttttgaaag attcctgatc
     8761 ccatttggcg ttgggatcaa tattaggttg aatcaagccg acattgacta aacgccggtc
     8821 gttttcgtct gatggttttt gcaagacgac cattccatag attgctggaa ttaaaaatag
     8881 ggcgataatt atcaccatgc gccgactcac ctgacggtaa tcttcgagat tttccagcaa
     8941 gcgaaaaata aggacattaa tgaccactat ccagcccgaa attccatagg gaccaaccac
     9001 agccgccgat tggattaact gaatggcatg cgactgagtg taacatagat tattccaggt
     9061 aaaacctaat gagccaaagg aacgcagata ttcgatagca gtccagataa tcggaaatga
     9121 ccagatgccg acgttccgtc ccagtcgccg gccgataaat ccgacgctca aaccgatgat
     9181 agcataattc atagccagaa aaataacagc cgctaccata gataaagtcg cccagtacca
     9241 ggtagttcct ttatttaacg ccaaccagta cataatcgcc aaattcagca ccagcccata
     9301 aaccaatcct ttttcaaaac cattatgaaa ttcatcatca caaaaagctt taatcaaagg
     9361 aaccaaggcg aaaaaagcca aaaatcccag cggcaagggc ggaaaagcca atccacttaa
     9421 tacaccagaa attaatatca accaccaaag gttgattttg actttcatca tctttggctc
     9481 agagtttttt ataaaggtga agtacttttt cggcgtcttt tacgctttct tcatcctttc
     9541 tttcagtagc cgaccgggtt tgaaatgagt tttgcggcga gcaggaacat atacttcttc
     9601 gttagtgcgc ggattgcggg ctttgggttt ggctttagtc ggtttgactt caaaaacacc
     9661 aaaattacga atttcaatcc ggattctcgg gtcagattca ctgagcattt cgcgcagaac
     9721 cataaagaca ccgtccacaa tctcggcagt gtcaaccaat ttggtatcaa gcgcttcagc
     9781 tgtgcgtctg acgacatctt ttttggtgta agtttttact ttcattttct aatctccttt
     9841 tgtttattta cgttctactg aaattatacc ctgcacattt tgcaatttcg ctaaaatttt
     9901 attgagatga cgcagatttt cgacctggat aaccagattc aaatgaataa ttgattcatc
     9961 aacgtcacca ctcacgctaa taatattcgt atcggtactt gccattatat cggtaacatc
    10021 cttcagaaat ttcttgcgtt cctgagccat tattttcaat ctgactaaaa aggcttgcgt
    10081 tcccgcgata tcccattcca cgttcaatag acgttcatca tcttctaata gagaaggaat
    10141 attcgaacag tcactccgat ggattatcac tccccgtccg cgactgacaa agccgatgat
    10201 ctggtcaccc ggaataggat tacaacattt ggcaaacgaa accatcaagt tacttatacc
    10261 ttgcaccttg atgcctttga ccgatttacg agccgtttcg ataaaaagtt catcgaggtc
    10321 ctgacgaacc agctgttctt ccttctgagg aaagagtttg gcataaatct gggggatagt
    10381 tagaatacca ctcccgaccg ccgcatacag tttatccagc tcgctgaatc cgagttcttc
    10441 gaacgattcc tgcacggttt tcaaaaatcg caattcttta ttgcgctggt tttctttctc
    10501 cagaatttcc ttcccgagat tgatactttc ttcgaattga gctttgcgta aataacgctt
    10561 gattgctcca attgctttgg cagtcgtgac aaatttcagc caggcataac tgggtcggtg
    10621 agaatcggaa gtgatgattt caatggtttg gccactgcgc aattcgctat ttagcggtac
    10681 catcttgccg tcaactttag ccccgataca gcgtgttcca atttcagtat ggacctcata
    10741 ggcaaaatca accggagttg cccccttagg taagcgaatc agatcgccca tcggcgtgaa
    10801 gacgaaaatc tcatctttaa aaagatcaat cttaagggta tccataaagt ctttggcgcc
    10861 ggtagattcg gtctgcagga tctcgattaa atcgcgtaac catttgacat atttatcaat
    10921 gtcgtccggt ttgtcgcgac cttccttata acgccaatga gcggcgatac cctcttcggc
    10981 gatctcatcc atttcggttg tccggatttg aatttccacc atacgaccac tgggaccgta
    11041 aaccgtagtg tgaatggatt ggtagaagtt ggctttgtgg ttggcgatat aatccttaaa
    11101 ccggtccata accggcgtaa aaagatcgtg aacaattccc agcaccgtat agcaattttc
    11161 tttagtatca actattatcc ggactgcaag caggtcaaag atttcctcaa aaggtttatt
    11221 ttgagttttc atcttcttat aaatcgaata aaaattcttc gggcgaccga caatcttggc
    11281 tttaatgccc tgttcgttca aagccttaat aattggctgg gtgaagactt taatatattt
    11341 ttcgcgttca ctgcgttttt cgttaacctt tttttcgaga aatcggtaag catccggatc
    11401 cagggctttc agaactaggt cttccatttc ggattttaac ttgaacatcc ccagacgatg
    11461 ggcaagcgga gcatatacat cgcgagtttc gatcgcaacc cgacgttgtt taatcggtgg
    11521 cagagaatcg agcgtttgca tattatgcag acgatcagcc aatttgatga ttagcacccg
    11581 gatgtcttca gcaacgctca aaagcatttt gcgaaaatta tcagcctgtt cttcttgccg
    11641 attgcgaaag gtaattccgc taattttggt aacaccctcg accaatttgg cgacatcatc
    11701 cccaaatttc tcagcaatat cttttactgt aacctcagta tcttcgatga catcgtgtaa
    11761 taatccaccg gcaaccgtta tcgggtccat atgcagatcc gccaaaatca aagcagtgtg
    11821 ataaggatgt tcaaaaaaac tgtccccgga ttgacgacgt tgaccgcggt gcgagttacg
    11881 tgcaaaacta tatacttccc aaagaaaatc ctcctcatct ttagttaaag gcgcgcgctg
    11941 attcaactta ctgatcagag taagaaattt ctggggacgc tgtttcgcgc gcggtaggac
    12001 tttattgagt atttcagttg ccattaaaat ggagtcgtta tgatttcttc tctttgggca
    12061 cccttatttt caaagcgagt atatttatca ataaagataa cctcgaccgt gtcggtcggg
    12121 ccattgcgtt gtttagcgac tatgatttcg gcgagacgtt catcttcgtc cgatgcggca
    12181 ctaccttcac gcttatcgta aaacgatttg cgataaatga atatcaccac atcggcatcc
    12241 tgttccagcg aaccgctttc ccgcagatcg gatagttgcg gtcggtgatc ggcgcggcct
    12301 tcgaccgctc gtgataattg cgaaagagcg atgactggta caccgatttc tttagataag
    12361 gctttcagtc cacgtgaaat ctcggtgatt tcctgctggc gattttccgc ccgatggccc
    12421 ttaatgagtt gcagataatc tataatcacc agctggatgt cgtgctcggc tttggcacgt
    12481 cgggctttgg cgcggatatc catcattgtc agcaccggcg aatcatcaat atagatttta
    12541 gcgttggcta aacgcccagt ctgcatactt agcctttgcc attgctcctt cggcaattta
    12601 cctttgcgta ctaaatggct atccagcctt gcttcggcac ataggattcg taaagccagt
    12661 tggtaatcag ccatttcaag actaaagaaa gcgactggca tatcatattc tacggcggca
    12721 ttgcgtgcaa ttgtcaaagc caaagcggtt ttgcccatac ccggtcggcc agccagaatt
    12781 atcaattctc ccggatgaaa gccggaggtc aattcatcta aatccaagag tccagtcggc
    12841 acgccactcg tatgcccttt gcgactgtga atcgaatcaa tgtgttcgaa tgtgcgattg
    12901 agaacgtcca cgaaattttt aaagccgcct ttcatccggt tttcggaaat cgcaaatatc
    12961 ttctgttcgg cggtatctaa aatatcctgc accgaatcgc gcgcttcata agattcggtt
    13021 tggatttcat tagaagccac gatcaggcga cgtaaaattg ccatatcgag gacgatattg
    13081 gcataatatt caacattagc ggctgaagga gtagcctcaa ccagtccggt gacataataa
    13141 gcgccaccga cctgctccag catttttttg cgtttgagct ggtctataac gctaacctga
    13201 tcaaccggct gtccttcatt aaagaggtca gtcatagctt gaaaaatatg ctggtgagct
    13261 tctttataaa aagcggtata atcgaggtac tggagagctt tactaaccgc ctctttatca
    13321 agcatcatcg ccccgagcac ggaaacttcg gcttcgtcgg aatgcggagg gactctgatt
    13381 atttcaccgt catttaaagc catttattct cctgttttat ccaagtataa gttcttgatt
    13441 ttcttgaaat cttcccaggc atattttttc aaattctcat tgcgcaaaag ggctgccgga
    13501 tggtaagtca cgattaaatc cacattatga tactgaaaaa tctgaccgcg cattttctcc
    13561 agagtatatt cgactcgtaa taaagttttg gctgaggtca aaccgagaca tattatcaat
    13621 tcaggctgaa ttattgcgat ttgtttcaaa agataaggct cacacttttc aacctcgccg
    13681 acattggggg tacgattttc tggtggacgg catttcaaaa cattggcaat ataaacctga
    13741 gcgcgggtca agccaatcgc ctttaacatt ttatcgagca aattaccagc tcttcccaca
    13801 aacggtaatc cttgcaaatc ttcattaact cccggtgctt caccaataaa tagcagttta
    13861 gcatgaggat taccggttcc aaaaaccacc ttagtgcgag ttgcggatag atgacatcgt
    13921 cggcagtttt gacaaatgtg ttccagttcc gctagctgag tggcggcgtc ggttttcggt
    13981 gtagactcct cgccattttc cgaaattaaa tagatttcgg ggggaaataa ttcagcctgt
    14041 tgtttgaaaa atgttttgat atcgtcgtat agtctggtca tttaagtttt cttataattt
    14101 ttaataacag tcgaaatgat tgaatcggcg atgtcacgtt tactggctaa aggaaaaacc
    14161 cattcttgac catcttttga taataaagta acactattgg tttcatgccc gaaagccgca
    14221 ccgctcacgt tgggatcatt ccagacgata aaatccatct ctttgtcgtt cagtttggct
    14281 aatgaacgcg ccttggcacc aggatgctcg gcggattcaa tctcgacgct gaatccaacc
    14341 acgcatgcct gacgattatg tttgcgaaaa tccatgataa tatcaggcga atatttaagc
    14401 tttagctcac taatgccact ctctttttta atcttctgag tggcaatttg cacgggtgat
    14461 aaatcttcga cagccgccgc cataaataaa aaatcagctt cagccgcttc acttaggacg
    14521 gcggaacgca tttcgttcgc agtctcaact cgaattgtct ttacgcccac cgtatttttt
    14581 aaatcagtag gcccactaat tagaagtacg tcggcgcttt cgcggaatgc aatttccgct
    14641 agggcatagc ccattttgcc actcgaccga ttggagatga agcgaatcgg atcaatatat
    14701 tcgcgagttg gtccagccgt cacgataact tttttatctt tcagccattt attgccatta
    14761 aggcggcgat caatatgcgt cagaattgtc gtctcgtcag ccaggcgtcc gcgcccggtg
    14821 ctaccacagg ctaaggcgcc ttcctccggt tcgatgatgt cgtaaccgag tcgccgcaga
    14881 cggctgatat tctcctgagt aatcggatta gcatacatat tatcgttcat tgccagagca
    14941 aagaccgttt tgttcccgcc aaccagaatg attgtactaa gcaaatcatc agcgatacca
    15001 ttagcagttt tagcgacaat attggcggta gccggagaca ccagaatcaa atcggcttca
    15061 gtcgccagat caatatggcg agtctcaatt ttctgaccgg taaacatatc ggttaaaacg
    15121 ggtttgtggc agaaggtttc aaaaatcagc gggctcataa acttttgggc ggcgtcggtc
    15181 ataatcacgg taacatccgc cttatagtca ttgaccagac ggcgcaaaag cccagccgat
    15241 ttataaatgg aaatgccgcc ggttaagccg agaacgattt ttctattcac taaattcact
    15301 atcgaattcc tcaagtggtt tttctttttc gggggtcata tagcgatagt tgattttgcc
    15361 ttctaaaatt tcgttgatgg ctaacgtggt cggcttttcg agatagtccc aagccggttc
    15421 gtcggtgatt tccggtggag tttcttcgaa agtctcctct tgctcctcaa ccatcgtcgg
    15481 caagggatat tgagccatac gcaaggcatt aatctgacgc gcgcgcttgg cggcaacgac
    15541 caccagttcg aaaatatcgt ctgatttccg agttagtttg ctaaaaggaa gggtttcagt
    15601 cattttttaa gatgctcctc tatgatatta ataattttat tgacggtggc ttccagattg
    15661 tcattgacaa tttgaaagtc gaactgttca cttagttcca attcatctgg gattcgggct
    15721 ttccgtatag cgatagaact cgaatcatcc gtcccacgtt tctggagacg ccggtcgagt
    15781 tctgtatcgc tgggtggtaa aaggaagatg ctaattagct ggtcggggta attttttttc
    15841 aaattgagtg tgccttttac atcaaggtct aaaagtaaaa ttttgccatc tctcaaggct
    15901 tgttcgattg aatcttttaa agttccgtag taattgccga aaatctcctg ccattcagcc
    15961 attttacctt cgtgaatatt tttcagaaat tgctcacgac taatgaaatg ataatctacg
    16021 ccgtcgcgtt cattcggacg gggtggacga gtagtacagg aaacggaaaa aacaaaacgg
    16081 tcatcccttt tcattagttc atggcaaact gtggtcttac ctgttcccga tggagcggca
    16141 atagctacta aaagtccttt gggtttcata ttacgttctg gatttgttca cgaatttttt
    16201 caagctcatt tttgagactg acgaccagat gagaaattgc ggtactttct gattttgacc
    16261 caatggtagt aatctctcga ttcatttcct gtgctaaaaa attcatctgc ttgccgaccg
    16321 gttcttcgct ttgtaatagt aaggtaaatt gctgaatgtg actgcgcaga cgggtgcatt
    16381 cctcagtaat gtccaatttt ttggctaaaa ttccggcttc ttgaatcaag cgattttcat
    16441 caagttgaca attttggcac agttccgcca gactctgctg gtatttcgtg aaatattcct
    16501 ggcgcgattg ctgtgagagt ttttcaatct ccgaacattt ttccaagata aattgcaaat
    16561 ggctatgtaa ttctcgggta ataaattcgc cttcccggcg acgcatagct attagctcag
    16621 ctaccgcctg acgcagaagg tcttttacct gagttaaaag ttcagcaaca tcagtatccg
    16681 cggaatcggt tataaaaatt tccggatagg taagcaaatt ttgaagagta accggctctt
    16741 cgatttgcaa ttcgcgcgcc agatcacata gcgctcggta atatttggtc aaaaaagcag
    16801 tgtttaattg cacctcgggg gttttttcta aaccggtaat tgcgatagtg atgcggactc
    16861 gaccgcgccg cagatcgcta gctaccaagc gcttcaactc ctcagtgtat ggttcgagcg
    16921 gttcgggaag attgaaagtc aggtctagaa agcggttatt ttcggagtaa atctcaacgc
    16981 tgattacttg accgccgcta cggtactccg ttctactata acctgtcata ctttccaatt
    17041 gcaaatctcc tatcaaatca atggtaaaat tagttaatcc agtccaaact gtcaaattta
    17101 aaatcgccat ttaatcttta ttttatatta aattgcaacc tatttttatt tatgaagaaa
    17161 ccaagtggaa tttatcagcg cctcacgaac tatatagctg taaccggttg ccacccaaac
    17221 aacatccgct tggcgctttg ataaatgctc ttttagctct actttttaac atgtttaata
    17281 gctggttaca tattgaaaga ttggcacgcg aataccaaca attgatcggc ggtgcaattt
    17341 gcggaatgcc ctttacctat caaaaggatc atttgcatat tccgttggaa atggcgggag
    17401 aatggcactg cctgcatttt agcgttcaag cgcccctacc attttttact ttagaaagga
    17461 ctttgccgca accaaaacac aaggtcaatc tgcttcgtga cgtaaccgca aaagcaatta
    17521 ccgatttggt ttgccgtgaa aacgaccgcc aggttttaat tactttaaat agtgggatcg
    17581 cctaccttct attccatctc tatggaatca atggaaatgt atatcttctc gattcatccg
    17641 gcgtgagtcg ggattcattt aaaaaagcaa aaaagccggt agactttgag ttgagcaatt
    17701 tccgacctgc caatcgcctt tttcccgatt atgccgattt taccagttca ttgcgtcagc
    17761 attccgatca gtcactgatg acttttttca ccaaatcatt tcaaccgatt ttaccgaaaa
    17821 ttctcgtgac cgaaatttgc tatcgaacta atctggcgcc gcagtattca attatcaatt
    17881 tgacggacga acaggttgaa agactcgcta aaactatagc cgaattatca gacgaaattg
    17941 taacttcttt gccgcgaatt tatagcggcg aaccgccaat ttttagttta attgaactgc
    18001 aatcgctttc aaatcgcgaa ttcgccgagt acgactcagt tatcgccgct caaaaccatt
    18061 ttattcagga ttacctgaac ctctatcgct tgaaggaaaa gaaaaaaacc ttgttgagac
    18121 aaaccgagtt tctgctaagc actacccgga gaaaattgat caagcaacaa agcgaactgg
    18181 cggctctgcc gacggcggat aattatcggg aatgggctga tacccttatg gcgacggcgt
    18241 ccaccatacc gcatcatagc aattcggtaa tcttgcctca attaaccgat gaagcccgcc
    18301 agattaccat tccgcttgaa ccaaaactca atccgattca aaatgccgaa cggtattacg
    18361 ccaaatcacg tcaaatcgaa aaagcaaggg ctgaattgat tcaaaaaatt gccgaagcgg
    18421 aacagttaat tcagcggctg gaaaataccc aaaaacaaat cgccgaggct gacgacttca
    18481 aaactctacg atctatatcc agccattggc cggctcagcc gcaaagctcg attgaaaata
    18541 tccatcagcc ctttattaat atcaatttgg acggctggga aattttaatc ggcaagagcg
    18601 cccgtgataa cgacgaactt ctccagaaat tcgctcgacc gaacgacttc tggcttcatg
    18661 ctcagggcac gagtggatcg catattgttg ttcgtaatcc cggcaaggtg gcgagcttgc
    18721 cgcgtcctat tctggaaaaa gccgccgggc tagcggcttt tcatagcaaa gccaaacatt
    18781 cagcggtcgt gccggtgatt tttacccaat gtaagtacgt taccaaacca cggcactcgg
    18841 cacccggcgc agtaaccgtc aaattcgaga agacaattat ggcggaaccg ctcgacccta
    18901 aaaaatttct ctaaccac
//
LOCUS       DAOOMU010000040        18608 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_3049_length_18608_cov_22.266264, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000040 DAOOMU010000000
VERSION     DAOOMU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 18608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18608
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3049_length_18608_cov_22.266264"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..167)
                     /locus_tag="PLI55_05370"
     CDS             complement(<1..167)
                     /locus_tag="PLI55_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPB00297.1"
                     /translation="MTDWIAIKKDEKHIAREKAKARELRQSNWWKQKIAAGICYYCGK
                     KFKPQELTMDH"
     gene            complement(160..855)
                     /locus_tag="PLI55_05375"
     CDS             complement(160..855)
                     /locus_tag="PLI55_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPB00298.1"
                     /translation="MTPDIKSHKKQSALSTSQTVIAFQKKVRKHFHGNYLLHLPPGYA
                     ESQNDWPTIFFLHGSGERGNDPEIVRRQGLPQVIDKKPDFPFIVISPQCPANRWWSLE
                     WLDALFDEVVKLYRIDQKRIYLTGLSMGGFATWDWAIEHPERFAAIAPICGGGNPLEA
                     DKIKDVPTWVFHGAKDEIVPLQKSQEMVAALKAGGGKVKFTVYPNTGHDAWTKTYNNL
                     RLYEWFLKQTKND"
     gene            complement(930..2222)
                     /locus_tag="PLI55_05380"
     CDS             complement(930..2222)
                     /locus_tag="PLI55_05380"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPB00299.1"
                     /translation="MQIKAINEELLLQNAVIVDPFKDNIYEADILIGKGKIKNIEQKI
                     DPKNVKICEDLKGSYVAPGFIDLHTHLREPGNEDEETIATGVKAALAGGFTKICCMPN
                     TDPPIDTQEAVRFIYRQAENLLAEVYPIAAITKGRQGAELTEMVELANAGAVAFSDDG
                     APLKNAQIMRYALEYSRLTGKPIINHAEDIDLKADGLMNEGVVSTHLGLPGNPAIAEE
                     IMINRDLQLAKFTSARLHVPHVSTAGSVELIRQAKAEGVHVTAEVTPHHFSLTDEYMR
                     TFDANGKVAPPLRTEADRQALIAGLKDGTIDAIATDHAPHTIDTKETSLDLASYGMIG
                     LETAFALAMTNLVHPGHLTLLQLIKLLTVNPAAIMNIALPKLENGADANLTIFDPNQW
                     WVFNRKNIYSRSQNTPFIGAELTGRIKAVVTKSHYISL"
     gene            complement(2245..3165)
                     /locus_tag="PLI55_05385"
     CDS             complement(2245..3165)
                     /locus_tag="PLI55_05385"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPB00300.1"
                     /translation="MNLSIRHLLGLEGVPRDDISTILDTAFSFREVLERPIKRVPTLQ
                     GKTIVNLFFENSTRTRISFELAEKRLSADTVNFSSSGSSLAKGESLKDTVRNLYAMKT
                     DAVVMRHGVPGSAKLLTQFIDAVVINAGDGTHEHPTQALLDMMSLQEKFGSLDGLNIA
                     IIGDIRHSRVALSNIYGLLTMGANVALCGPPTFMPVGIESLGVKVIYDLDEALAFADA
                     INILRIQRERQGKGMIPSLREYRAVFGITRERLDKLHKTITILHPGPINRGVEIDSDV
                     ADSEHSLILHQVLNGVAVRMAVLFLLLGGK"
     gene            complement(3162..3710)
                     /gene="pyrR"
                     /locus_tag="PLI55_05390"
     CDS             complement(3162..3710)
                     /gene="pyrR"
                     /locus_tag="PLI55_05390"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPB00301.1"
                     /translation="MEFRIKARLADEQGVNRTITRLAHEIIENCKGTENVGIIGIRTR
                     GDYLAKRLAHKIEEIENVKLPVGVLDVVMYRDDFRMKTKLPRVEVTDIPFDVDGKILI
                     LVDDVIYTGRTIRAALDALMDFGRPAAIQLAVLVDRGHRELPIKPDYVGKNIPTSIGE
                     EVRVMIKEIDGRDEILLVEEQK"
     gene            complement(3936..4865)
                     /locus_tag="PLI55_05395"
     CDS             complement(3936..4865)
                     /locus_tag="PLI55_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00302.1"
                     /translation="MRQVVDFGFAWNWEYDWDFANLLEANCQSEGLNFLQVTPEKIKS
                     VLTELGQGGIYLQALWDRAADTDSRFIELINWAKNNNILQINPHEKAVRAWNKATMHL
                     EFITAGVNTPYTIVLDPYNVRPILPEIDLSVLGNRFIIKPAHGGGGLGVILDATSLER
                     VLTARRENPDDFYLLQAVIEPLQVNGRPAWFRVIYGFGKCYFCWWDVKTHIYTPFSNN
                     DKESLPMPLLNDIIEKIADVTGLDVFSSEIALTAEGKFVVVDYINDPVDLRLQSRAAD
                     GVPDEIVRAISSDCVATVKRAVQAKATYEKLEN"
     gene            complement(5213..5611)
                     /locus_tag="PLI55_05400"
     CDS             complement(5213..5611)
                     /locus_tag="PLI55_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00303.1"
                     /translation="MTEEQIRQIKLISYANWLVTEKFGAEKGIRDIKALTAAVTLPGN
                     GTLSCVLGERLSNRKDLYAQLGWFVYYMIEGEPFNSKNRTTVLLILLWVLKYYGLEFN
                     AEELANYIKTLDPLKHGNSDISLWFSTHCR"
     gene            complement(5604..5846)
                     /locus_tag="PLI55_05405"
     CDS             complement(5604..5846)
                     /locus_tag="PLI55_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPB00304.1"
                     /translation="MKRKIIRVGSSKGVLLPREVTKAMDWGFGSEIELSVDEEKHEVY
                     LKSLKVSVPQDYEIDHLRQLEEVLEDYADLLEGIDD"
     gene            complement(5861..6118)
                     /locus_tag="PLI55_05410"
     CDS             complement(5861..6118)
                     /locus_tag="PLI55_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPB00305.1"
                     /translation="MRRKIVRVGASLCVLLPKEVVRAMGWDFEDEINLILDEENEVVI
                     LRNLQNQPIEKKTMMEHFDKFLENYGKELDTLVADPEPQIQ"
     gene            complement(6379..7713)
                     /locus_tag="PLI55_05415"
     CDS             complement(6379..7713)
                     /locus_tag="PLI55_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPB00306.1"
                     /translation="MDLFEANPQNTNLLPPLAERMRPRRLEDFKGQEEIVAQGKILRK
                     ALESRKIFSMILWGPPGSGKTTLAKIIANYVDADFYQISAVSAGVKDVRAIIDKAAYN
                     RSRNRSTLLFIDEIHRFNKAQQDALLHAIEDGSLILIGATTENPSFEVIAPLISRCRV
                     LKLEGLSDENLASIINTAIAKDIILLKYKIKFEPGAQDFLIKNVNGDARKLLNAIEIC
                     QQIAAPEEGAEFTITIDLIKEALQQRRQLYDKTGDYHYDTISAFIKSVRGSDPDAAVY
                     WMALMLEGGEDPLFIARRLIILASEDIGNADPSALPLATAGFQAVHAIGMPEAAIVLA
                     QVTTYLASTFKSNASYMALENARQALKEGSAPNVPLHLRNAPTKLMKAQGYHKGYRYP
                     HSYPDHFVKENYFPCDGLAPTFYQPTSIGREKFLRERLEQLWPERYRRTKNS"
     gene            complement(7746..9137)
                     /locus_tag="PLI55_05420"
     CDS             complement(7746..9137)
                     /locus_tag="PLI55_05420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB00307.1"
                     /translation="MPSLTFLGATGTVTGSRFLLKTAHKQYLIDCGLFQGLKELRLRN
                     WEPFPISPEDIDAVILTHAHIDHTGYLPRLVHYGFKGTIYATTATTDLCQILLPDSAH
                     LQEEDAEYANKKHFSKHSPALPLYTTEDALKALELFKPIAYGSRLQLDDGVSLTFQDA
                     GHILGSSFIDLRLPIDGEERRLLFSGDLGRPNQPILRDPHTVFGADYLIVESTYGDRL
                     HGNEDPKEVMARIINESIRRGGVLLIPSFAVGRTQELLYTIRELEEANRIPHLPIYMD
                     SPLAIKATKIFSKNKQDFDLKSKTLELKGKNVLETAQLHFAQTQEESKAINKIKSNAI
                     IISASGMATGGRILHHLFNRLPDPKNTVLFIGYQAVGTRGRTILDGAESVKIHGQYVP
                     IRAHIESTSCFSAHADYHEILAWLSNFNSVPRKVFIVHGEPEPARSLSDKINRHFGWS
                     TELPEYLSQYQIS"
     gene            complement(9494..9916)
                     /locus_tag="PLI55_05425"
     CDS             complement(9494..9916)
                     /locus_tag="PLI55_05425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014455682.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HPB00308.1"
                     /translation="MNIGLIGPAAALGLAALGSVLGCGIAAQTAVGGWKKCFANNKPA
                     PFMLVAFAGAPLTQTIYGFLLMLFINGSDQEPLVKLCAGVFGGLAIGFSAFFQGKVAA
                     RAADALAETGKGTANYFMVIGIVETIAVFVLVFMLLNL"
     gene            complement(9913..11751)
                     /locus_tag="PLI55_05430"
     CDS             complement(9913..11751)
                     /locus_tag="PLI55_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPB00309.1"
                     /translation="MKKVVLFVHTPERDSALKKLRKLGVVHIQHIKPPISEDTSKIEN
                     AIYAVEKSLGIIEGYNTPPSSENVDLEKCQKLVSDIIALETEKQNLKSKIDEKNIYLN
                     WYNRWGIISEKDVAELKEHRIYLHLYVIDRSILKNIPEEAAVEILKEEKNQLLIAFIA
                     RSEDVRLDLKEEQIPPEDFDTVTRELQNFTSRLAEIEQQLTELAKKAGLIRAYQQELE
                     KHLEFARVRSGMQDAEEITYLQGYCPSDDVSQLMQAASDEGWGILSADPDETDNPPTL
                     LKMNKIARLIQPVLDFLGTVPGYREYDISQYFLIFFALFVAMIIGDAGYGLIFFLVAI
                     FAHYKLVKQGKSLPLFLKLLYLLSICTIGWGTITGNWFGSIQIAGLPLLKALTIPQLA
                     TFPELFPDLTVNPQDQIMFICFTVGVTQLGLANIMNFINNLPHLKALGNLGWFGVTVG
                     AFLLVLNLVLGSPLPSFTVPLIGCSVVAIVLFMRQESGTSFLKGIARGLGDGFATFLN
                     VISSFSNIISYIRLFAVGMATVAIATSFNQIAASLLKGFTLPAGMLILIIGHGLNIVM
                     SILSVIVHGIRLNVLEFSSQLGMEWSGYKYEPFREKIKNLTEREIQ"
     gene            complement(11760..12371)
                     /locus_tag="PLI55_05435"
     CDS             complement(11760..12371)
                     /locus_tag="PLI55_05435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPB00310.1"
                     /translation="MAKIKHTKNELKAQREALARYERFLPTLELKKSQLVTEIRHLSL
                     AIEQVEEEIRKVTEQVFTWVDVMAEEVDLKAWLKIKEIHTDEGNIAGIEIRIFRQVEF
                     EDVPYDFFTTPLWVDRALSILKEQIARRIKIKILQEQIEVLQQELRVTIQRINLFEKV
                     KIPETRENIRIIQIFLGDAQTAEVVRGKIAKSKIQSKREALKV"
     gene            complement(12387..13697)
                     /locus_tag="PLI55_05440"
     CDS             complement(12387..13697)
                     /locus_tag="PLI55_05440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPB00311.1"
                     /translation="MQKVYNKILSVTGNVITVKASNVANEELAEISTQRGKSLAQVIK
                     LDGDIVSLQVFAGSQGIATNDEIRFLGHPMQVTFSDNMLGRIFNGSGVPRDKGPQLTE
                     NMIQIAGPSVNPAKRIIPRRMVRTNIPMIDVFNSLVISQKLPIFSVSGEPYNELLARI
                     ALQAEVDMIILGGIGLKYDQYMFFKNTIEEGGALSRSIFFIHTASDPIVEGLMVPDLS
                     LAVAERFALKGRDVLVLLTDMTNFADALKEIAITMEQVPSNRGYPGDLYSQLAARYEK
                     AVDFEGAGSVTILAVTTMPGDDVTHPVPDNTGYITEGQFYLRNGRIEPFGSLSRLKQQ
                     VNKSTREDHRAIMDGMIQLYAQFKETEEKRSMGFRMSDWDRKLLKYGERFENDLMDLK
                     VNIPLESALNLGWQILADCFEPQETGMKSELVQKFWPKKAEVSA"
     gene            complement(13681..15420)
                     /locus_tag="PLI55_05445"
     CDS             complement(13681..15420)
                     /locus_tag="PLI55_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPB00312.1"
                     /translation="MKKYGKITSINGNMITVACESDIIQNEVGYAVVGDENLKAEVIR
                     IQGNLAYMQVFESTKGLRVGDKVEFSGQMLSVRLGPGLLGMVYDGLQNPLHDLAAKFG
                     FFLKRGAVIEPLDSKKEWDFKPLVKSGDIVQAGISLGNVSEGIFEHQIFVPFNLRGEY
                     TVKTIVPEGNYKITETIAVITNEKGDEINLKMNFEWPVKIPITAYREKFVPTEPLVTQ
                     TRIIDTFFPISKGGTYCIPGPFGAGKTVLQQVTSRFAEADVVIIAACGERAGEVVETL
                     REFPELTDPRTGRSLMDRTIIICNTSSMPVAARDASVYTAATIGEYYRQMGLNVLLLA
                     DSTSRWAQAMRELSGRLEEIPGEEAFPAYLESRIAQFYERAGIVELLDGSRGSLTIGG
                     TVSPAGGNFEEPVTQSTLKVVGAFHGLSRDRSNARRYPAIHPLESWSKYTSFIPSNLI
                     EDARQILFKGNEVHQMMLVVGEEGTSLDDFEIYMKSEFLDAVYLQQNSFDQVDGASSP
                     ERQKYVFSKVHEVLMTKFNLTDKNDARDFFNKLRQVFIDWNYIDFKDDEFKIQEQKIN
                     QLIKEKARDAKSL"
     gene            complement(15417..15914)
                     /locus_tag="PLI55_05450"
     CDS             complement(15417..15914)
                     /locus_tag="PLI55_05450"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HPB00313.1"
                     /translation="MDKYYYLIAQLPVLYFEREPALTIEQFLTEAYKWLSAEDYAQLE
                     KVDLNATAPDKNDPDIIRRIKNFELDLRLELVEWRTAQKKNQDYKIIAFPANLVREGD
                     PLTVEKNLLLWRWQFLDSLEFGHYFDLEFLIMYYLRLQILKRLFTFNKKIGREKYQQY
                     LEMEL"
     gene            complement(15907..16506)
                     /locus_tag="PLI55_05455"
     CDS             complement(15907..16506)
                     /locus_tag="PLI55_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00314.1"
                     /translation="MDVKLDSLIEKIKQEGIEEAQRAADEIRQKAEAEAQEIINKANE
                     KARQIINEGQQNAEKLRANAEAAIRQAARDTILVTKENLLKLFDRVFKKEIAKTLTPE
                     FLKEMILALVKSTSSDNQYEFVVAEKDIEKLKTLVLAATRDSLKEPVTFRVDRGIAAG
                     FRVSRKGEEVYYDLTDQSIAEVLQEFLNPALRQLLDQNG"
     gene            complement(16717..18183)
                     /locus_tag="PLI55_05460"
     CDS             complement(16717..18183)
                     /locus_tag="PLI55_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00315.1"
                     /translation="MRLIHQFFVGKSHYLFPTFFLTVSLSNLAFASHWQASLYNLLAF
                     PKIPTVTLAELNSPSLPFIKARIGYDDIYRKKVDQQTTLTISEQTNTLQLNLPFRLRQ
                     TSHLIKAEAARNFSNFDNLSDQTSLVSHCQIDNTYFSTLWAMAYERIHFGGGLEYNRN
                     SAPFLINIISFPHSEDELTNKYFFDLLEPTFGEELTNTLHVNQIGYSLWTSLPLNSNY
                     HLGLAIRATDFDFHWRIRYINTGQEAALAGQRQIDLPINGLNHYYRLALNADQSFVKD
                     ISVMFLRNDFDYFIDNNPPNFTDLDSLGQGYIDREGYAIQFTLGEKLFEISGGLSYAN
                     YESDIFLSTPVLGFQLGILPIFHSAELNFSYGHSFSQQIGFGTNFNWQNINFHAKALY
                     SHTLFDFWLEGKAKLMMGIKSDPLDYPYKYSLHLFDLNAEAEWRRGPLGIAYTFQQLI
                     PSGRRMDDSPIRLSKKVPGVDYTYRGGQQHRINLAYYF"
     gene            complement(18192..>18608)
                     /locus_tag="PLI55_05465"
     CDS             complement(18192..>18608)
                     /locus_tag="PLI55_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB00316.1"
                     /translation="ERVNVSAWFDNPPTSFLVMMKNFFMGTDSTLGGMFPDRFKVGIA
                     QENQALPTEFKLYPVYPNPFNPVANITFDLPQAADVKLCIVDLQGRVIEQIVNRRISA
                     GRISYDWNASHYPSGIYFAMIEVNGHRSIRKMTLLK"
BASE COUNT         5153 a         4273 c         3854 g         5328 t
ORIGIN      
        1 atatgatcca tcgttaattc ctgcggctta aatttcttgc cgcagtaata gcatatcccg
       61 gccgctattt tctgtttcca ccagttggat tggcgtaatt ctctggcttt cgctttttcg
      121 cgagcaatat gcttctcgtc tttttttatt gcgatccagt cagtcatttt ttgtctgctt
      181 tagaaaccat tcgtagagcc taagattatt ataagtcttc gtccaggcgt cgtgaccggt
      241 gttcggataa acagtaaatt tcacttttcc gccgccagct ttcagcgccg caaccatctc
      301 ttgtgatttt tgaagcggca cgatttcatc tttggcacca tgaaaaaccc aggtcggaac
      361 atcttttatt ttgtcggctt cgagcggatt gccaccgccg caaatcggcg ctatcgccgc
      421 aaaacgttcc gggtgttcga tagcccagtc ccaggtagca aagccgccca tactcaatcc
      481 ggttaaataa atacgtttct gatcaattcg atagagcttg accacttcat cgaatagtgc
      541 gtccagccat tccagcgacc accagcgatt ggcgggacat tgaggcgaga ttacgatgaa
      601 cggaaaatct ggttttttat caataacttg ggggagtccc tggcgccgga cgatttcggg
      661 atcgttccct cgttcaccgg agccgtgtag gaagaagatc gttggccagt cattttgaga
      721 ttcggcataa ccgggcggca aatggagcag gtaatttccg tggaaatgct tccggacttt
      781 cttttggaag gctatcactg tttgagaggt tgatagcgct gattgttttt tatgcgattt
      841 gatgtctggc gtcattcgac aattaattta aataagatta agcgtatcct caactttcag
      901 gaatttcgct ttaggtttgg tgagagtact tataatgaaa tgtaatgcga tttggtgacg
      961 accgctttga tccgtccggt cagttccgcc ccgataaaag gggtattctg agagcgcgaa
     1021 taaatatttt tacgattgaa aacccaccac tggttgggat cgaaaatcgt cagattagcg
     1081 tccgcgccgt tttctaattt cggcagggcg atgttcatta tcgcggcggg attgaccgtc
     1141 agcaatttta tcaattgcaa aagagtcaaa tgccccggat gtaccaaatt ggtcatagcc
     1201 agcgcaaaag cggtttccaa tccgatcatc ccatacgatg cgaggtctaa cgaagtttct
     1261 ttggtgtcaa tcgtatgggg cgcatggtcg gtagcgatag cgtcaatcgt gccgtcctta
     1321 agcccggcaa tcaaagcctg acgatcggct tcggttcgca gaggtggagc gactttgcca
     1381 ttggcatcaa aagtgcgcat atattcgtct gtcaaggaaa aatgatgcgg agtaacttca
     1441 gcggtcacgt gtacaccttc ggctttagct tggcggatca attccacgga accggcggtc
     1501 gagacatgcg gaacgtgtaa acgcgctgag gtaaatttcg ccaattgcaa atcgcgatta
     1561 atcattattt cctcagcgat agcaggatta cccggtaatc ccagatgagt cgagacaacg
     1621 ccttcattca tcaaaccatc ggcttttaaa tcaatgtctt cggcatggtt gataattggt
     1681 ttgccggtca atcgggaata ttcgagagca tagcgcataa tctgggcgtt tttcaaggga
     1741 gcgccgtcat cggagaaagc caccgcacca gcattagcta attcgaccat ttcggtcagc
     1801 tcggcgccct gacgaccttt ggtgattgcc gcaatcggat aaacttccgc caacaggttt
     1861 tcggcttgcc ggtagataaa acgtacggct tcttgagtat caatgggcgg atcggtgttc
     1921 ggcatacagc aaattttagt gaatccgcca gccagcgcgg ctttgactcc ggttgcaatt
     1981 gtctcctcat cttcattgcc cggctcgcgt agatgagtat gcaggtcaat aaatcccggc
     2041 gcgacgtatg atcccttgag gtcttcacat atttttacat ttttcgggtc aatcttttgc
     2101 tcaatatttt ttattttacc ttttccaatc agaatgtccg cctcataaat gttatctttg
     2161 aatggatcaa cgatcaccgc attctgtaaa agtagttctt cgttaatggc tttaatttgc
     2221 atttttactc ctaaataatt ccatttattt gccacccaat aatagaaaaa gtactgccat
     2281 acgcacggcg acgccgttta gcacctgatg aaggataagt gagtgctcgc tatcggcgac
     2341 gtcagagtca atttccactc cgcgattaat cgggccggga tgtagtatcg taatcgtttt
     2401 atgcaattta tccaggcgtt cgcgcgtgat gccaaaaacc gctcgatatt cgcgtagtga
     2461 tgggatcata cctttacctt gccgttcccg ctgaatgcgc aggatgttga tagcatcggc
     2521 aaaggctaaa gcttcgtcca gatcatagat tacttttacg cccagagatt cgattccgac
     2581 cggcataaac gtcggtggtc cgcagagtgc gacattagct cccatagtta acaatccgta
     2641 aatattagag agcgctactc ggctgtggcg aatgtcaccg atgattgcga tattcaggcc
     2701 gtcgagtgaa ccgaattttt cctgcaatga catcatatcc agaagggctt gggtcggatg
     2761 ttcatgagtt ccgtcaccgg cattgattac aacagcatct atgaattgcg tcagtagttt
     2821 ggctgagccg ggcacgccgt gtcgcatcac gacagcatct gttttcatag catagaggtt
     2881 gcggacagta tctttaaggc tttcgccctt ggcgaggcta cttccgctgg atgaaaaatt
     2941 gaccgtatcg gctgaaagac gcttctctgc cagttcaaat gatatgcgcg tcctggtgga
     3001 attctcgaag aaaagattga caatggtctt gccctgcaga gtcggaacac gcttaatcgg
     3061 gcgctctaga acttcccgga atgaaaaagc ggtgtccaga atggtactga tgtcatcgcg
     3121 cggcacacct tccaggccga gtaaatgcct aatactcaaa ttcatttctg ttcctccact
     3181 aacaggattt catctctacc gtcgatttct ttaatcatta cgcgaacttc ttcaccaatc
     3241 gaagtaggta tgtttttccc aacataatcg ggtttgatcg gtagttcgcg atgaccgcgg
     3301 tccaccagca ctgccaattg aattgccgcc ggtcgcccga aatccatcag agcatccaga
     3361 gcggcgcgaa ttgttctgcc ggtataaatt acatcatcta ccaggattag gattttgccg
     3421 tccacatcga aaggaatatc ggtgacctca acgcgcggca atttggtttt cattctaaaa
     3481 tcatcccgat acataacgac atcaaggaca ccgactggta gtttgacatt ttcgatctct
     3541 tcgattttat gggctaaacg tttggctaaa taatcaccac gcgtgcgaat accaatgatt
     3601 ccgacatttt cggttccttt gcaattctcg ataatttcat gggctaatcg cgtaatcgtg
     3661 cgatttactc cctgctcgtc cgccaggcgg gctttaattc taaattccat agattgctcc
     3721 taaaatgaaa aaaccaccgc tctgggtgga tttctggttt ctcttttgcc tttcccaact
     3781 cacggtttgg ctttaaaggc tccttttttt aattacgtta atttagatga tagtcataat
     3841 aaagccaaag tatttctcgt aatcaaaaaa tctataatca tagataaaca gttagatttc
     3901 tctggattga ataaaataac aacttaccca atcctctaat tttccagttt ctcataagtt
     3961 gcttttgcct gaacagctct ttttacagtg gcaacgcaat ccgatgaaat cgctcggacg
     4021 atttcgtccg ggacaccatc tgccgctctg gattgcaaac gcaaatcaac tggatcgtta
     4081 atataatcta ccaccacaaa tttaccttct gccgtcagag caatttcgga ggagaaaaca
     4141 tccaatcccg taacatcggc aattttctcg attatatcgt ttagtaatgg cattggcagg
     4201 ctctctttat cattattcga aaaaggggta taaatatgcg tctttacatc ccaccagcaa
     4261 aaatagcatt ttccgaagcc ataaatgacc cgaaaccaag ccggccgtcc gttgacctgt
     4321 aacggttcga ttacagcctg taaaagataa aaatcatccg gattttctcg acgagcagtc
     4381 aatacgcgct ccagactggt cgcgtccaga attacaccca agccaccacc accatgcgcc
     4441 ggtttgatta taaagcgatt gcccagaacg ctcaagtcaa tctcgggcaa tatcggtcgg
     4501 acattataag ggtccaaaac tatcgtataa ggtgtattga ctccggctgt gataaattcc
     4561 aggtgcatcg tggctttgtt ccaggctcgg acggcttttt cgtgaggatt aatctggaga
     4621 atattgttat tcttagccca gtttatcagt tctataaatc ggctatcggt gtcagccgct
     4681 ctatcccata atgcttgtaa ataaattcca ccttgcccta attcagtcag taccgatttg
     4741 atcttttctg gggtcacttg aagaaaattt aatccttccg actggcaatt cgcttctaat
     4801 aaattggcaa aatcccaatc atattcccag ttccaggcaa agccgaaatc aactacctgc
     4861 cgcatctaaa attcacttct cggttatcaa atgcaggacg atattaggat tattcatcaa
     4921 gttgcacaaa ggataatatt ttaaataaaa ccaagtatta tcactaatca tttaacaaaa
     4981 gttaaggttt ttaaacgagc cgtcaaaagt attactcgat aaaattttac tatccggtaa
     5041 tgaaccaagg aatagaaaac taactcttcc agctttatga gcccaatttt ttaccgctat
     5101 tggtttctta acggtttcat ccatcgtgac ttttataaaa aaattaatta tatacccatt
     5161 acagaggaca agattatttg agcggaattt ttagcagaaa aaagtaagtg atttaacgac
     5221 aatgagtcga gaaccagagg gaaatgtcgg aattgccatg cttcagagga tcgagggttt
     5281 taatatagtt ggcgagttct tcggcattga attcgaggcc gtaatatttt aatacccata
     5341 ataaaattag aagcaccgtt gtgcgattct tgctattaaa cggctcgcct tcgatcatat
     5401 aataaacaaa ccaaccgagc tgggcataca aatccttgcg attggaaaga cgttcaccca
     5461 gaacgcagga aagggttcca ttccccggta aggttacagc cgcagttaga gccttgatgt
     5521 ctcggatgcc tttttcagcg ccaaatttct cggttaccaa ccagttagca taagatatca
     5581 acttaatttg cctgatttgt tcttcagtca tcgataccct ccaacaaatc agcataatct
     5641 tccaatactt cctcgagctg tcgcaggtgg tctatctcat aatcttgcgg cacgctgact
     5701 tttaaactct tcaaataaac ctcgtgcttc tcttcatcaa cactaagctc gatttcggaa
     5761 ccaaaacccc aatccatcgc cttagtcacc tcgcgaggta gtaagactcc tttactggaa
     5821 cccacccgaa ttattttcct tttcatcttt tttcccctaa ttattggatt tgaggctccg
     5881 gatcggcaac cagagtatcc agctcctttc cgtaattttc gagaaacttg tcgaaatgct
     5941 ccatcatcgt ttttttctcg attggctgat tttgcaggtt gcggagaatt accacttcat
     6001 tttcctcgtc taggatcaaa ttgatttcat cttcgaaatc ccatcccata gctctaacta
     6061 cctctttggg taatagcaca cataggctgg cgccaactct aactattttt cttctcatta
     6121 catcgcctct atagcgtttt gtaaattaca tctcaaaata aattaacaat tagaaataat
     6181 caagcttttt ttataaatat acttattagg atgcttcatt tggtattaaa tagcctattt
     6241 aaagtcaaaa taactatcac aatggttgtt atgttcatca gagatataaa ttattatttt
     6301 tagatccaaa tatctgatat tcactgataa ttatttaata atcccaagcg aaaaattgta
     6361 tagataaata aaattatttc aggaattttt cgtccttcgg taacgctcag gccagagctg
     6421 ctcaaggcgc tcgcgtagga atttttcacg accgatactg gtcggttgat aaaacgtcgg
     6481 cgccagtcca tcacagggga aataattctc tttcacaaaa tggtctggat aactatgggg
     6541 ataacgatat cccttatgat aaccttgggc tttcattaat ttggttggcg cattgcgcaa
     6601 atgcagggga acgttgggcg cactgccttc tttgagtgcc tggcgagcat tttctaatgc
     6661 catataggaa gcgttacttt tgaacgtgga tgccagatag gtcgtgactt gggcaagaac
     6721 aatagcggct tcgggcatac cgatagcatg tacagcctga aaaccagcgg tcgctaacgg
     6781 caaagccgat ggatcggcat tgcctatatc ttcacttgcc agtataatca aacggcgagc
     6841 gatgaatagc gggtcctcac caccttctaa catcaaagcc atccagtaaa cggcggcatc
     6901 cggatcactg ccgcgcacgc ttttgatgaa agccgaaata gtatcatagt gataatcacc
     6961 agtcttatcg tataactgcc ttctttgctg gagtgcttct ttaattaaat caatagtgat
     7021 agtaaattca gcgccttcct cgggcgccgc tatctgctgg caaatttcga tcgcattcaa
     7081 caatttgcga gcatcgccat tgacattttt tatcaaaaaa tcctgagcac ccggctcaaa
     7141 tttaatttta tactttaata gaataatgtc tttagcgata gccgtattga taatacttgc
     7201 cagattttca tcagataatc cctccagctt caacacccgg caccgcgaga tgagcggcgc
     7261 aatcacttca aaagaggggt tttcggtcgt ggctccgatt agaattaacg atccatcttc
     7321 aatggcatgg aggagagcat cctgttgagc tttgttgaag cggtgaattt catcaataaa
     7381 caataaggtc gagcgattgc gactacgatt ataagccgct ttatcaataa tagcccgcac
     7441 atccttcact ccggctgaaa cggcactgat ctggtaaaaa tcggcatcca cataattagc
     7501 aattattttg gctaaagtag tttttccact gcctggaggt ccccatagaa tcatcgaaaa
     7561 aattttccgg gattctaaag ctttccgcaa aattttgccc tgcgccacga tttcctcttg
     7621 ccctttaaaa tcctcaaggc gtcggggacg cattcgctcc gctaagggcg gtaacaaatt
     7681 tgtattttgc ggattcgctt cgaacaaatc cattgatatt tttccttgcc taattatacc
     7741 acattttagc tgatctgata ttggctcagg tattccggca attcggttga ccagccgaaa
     7801 tgccggttga ttttgtcact caaactacgg gcaggttccg gttcgccatg gacaataaaa
     7861 acttttcgtg gcacagaatt aaaattcgat agccaggcta aaatctcgtg gtaatcggcg
     7921 tgggctgaaa agcaggatgt actttcaata tgcgcccgaa tcgggacata ttgcccgtga
     7981 atcttgacac tttcggcgcc atcgaggatc gtgcgaccac gcgtgccaac tgcctggtag
     8041 ccaataaata ggacggtatt tttcggatcg ggtaggcgat tgaaaagatg gtgcaggatt
     8101 ctgccgccgg tagccatacc gctggcagaa atgatgatgg cattgctttt tattttattt
     8161 atggctttgg actcttcctg ggtttgcgca aagtgcaatt gagccgtttc taatacattc
     8221 ttgcccttta attctaaagt tttagatttc agatcgaaat cctgcttgtt tttagaaaat
     8281 attttcgtag ccttgatggc taaggggctg tccatataga ttggtaaatg cgggattcga
     8341 ttagcttctt ccaattcacg gattgtataa agtaactctt gagtccgacc tacggcaaaa
     8401 gatggtatca gcaacactcc gccgcgtcgg atactttcgt tgattatgcg cgccattact
     8461 tctttcgggt cttcattacc gtgcaagcga tcgccgtaag ttgactcgac aattagataa
     8521 tcggcaccaa aaaccgtatg cggatcgcgt aaaataggtt gatttggtcg gcctaaatca
     8581 ccggaaaata acaaacgtcg ttcttcaccg tcaataggca aacgcagatc aataaaagaa
     8641 gaccccagaa tatgaccagc atcttgaaag gttagagaaa ctccatcatc caattgtaaa
     8701 cgactaccat aggcaatcgg tttaaataat tccagagctt tcagagcatc ttccgtagtg
     8761 taaagtggca aggctggact gtgtttggaa aaatgttttt tattggcata ttccgcatct
     8821 tcttcttgca aatgagccga atcaggtaac aatatctggc aaaggtcagt tgtagccgtt
     8881 gtggcatata ttgtcccctt aaaaccataa tgtaccagcc gtggcaaata accggtatgg
     8941 tcaatatgag catgcgtaag gatcacagca tcaatatctt ccggcgaaat aggaaacggt
     9001 tcccagttcc gtaaacgtaa ctcttttaaa ccttgaaata aaccgcaatc aattagatat
     9061 tgcttatggg cagtttttaa caaaaaacgt gaaccggtta ccgtaccggt cgcgccgaga
     9121 aatgttagac ttggcatctt gtctctctta aattgtcacc agtttttaaa tttttcaagc
     9181 gattaatcga ataatcgttc atcaaaaaga atatattgaa ctctcaggag gtttccaata
     9241 tgtagctggt tatttaatca gaagtattca agcccggctt tcttttatta aacacatctc
     9301 ccggtaaaag taaaaacttc tgtaaaatat tatcagagcc cttttcacta aagacgctat
     9361 cagagagtta atttccctaa tacattatca atttatttta gtaaaacttt ttagaccttc
     9421 ttcgaactac cagcaactgg ctgagattct ataattacca gccgactaaa gcgtagtgat
     9481 tctaagcacc gatttataag ttcagcaaca tgaaaactaa aacgaacaca gcaatagtct
     9541 ctacgatacc aattaccata aaataattgg ctgtcccttt acccgtttca gccagtgcat
     9601 ccgcggcgcg ggcggcaact ttaccctgaa aaaaagccga aaaaccaatt gccaaacccc
     9661 caaaaacacc cgcacagagc ttaaccaaag gctcttgatc acttccattg ataaaaagca
     9721 tcaaaagaaa cccataaatt gtctgcgtta gtggcgcacc ggcaaaagca accagcataa
     9781 aaggcgccgg cttgttgttg gcgaagcatt tcttccagcc tccgacggca gtttgggcgg
     9841 ctataccgca tcccagaacc gagccaagag cggctaatcc taaagcggct gccggaccga
     9901 tcaatccaat attcattgta tttccctttc tgttaaattt tttatttttt ctctgaatgg
     9961 ctcatattta tagccagacc attccatacc taattggctg gaaaattcga ggacatttaa
    10021 ccgaatacca tgcacaatta ctgatagaat actcataacg atgttcagtc catgtccgat
    10081 aattaatatc aacattccag ccggtaaagt aaaacctttc aataacgatg cggcaatctg
    10141 gttaaaactg gtagcaattg ccacggtcgc catacccaca gcgaagagcc gtatatagga
    10201 aataatattt gaaaacgaac tgataacgtt caaaaaagtc gcaaatccgt cccctaatcc
    10261 ccgcgcgatt ccttttaaga agctggtacc actctcctgg cgcataaata aaactattgc
    10321 cactacacta cagccaatca gaggcaccgt aaatgaaggc aacggcgatc ccaaaactaa
    10381 atttaatacc aacagaaagg cacctaccgt tactccaaac catcccagat tacccagagc
    10441 ttttaaatgc ggcaagttat tgataaaatt cataatatta gctagcccca gttgcgttac
    10501 accgacggta aaacaaataa acataatttg atcctgggga ttgaccgtca ggtcgggaaa
    10561 taattccgga aaagttgcca gctgaggtat cgtcaaagcc tttaataatg gcaacccggc
    10621 aatctgaata gatccaaacc agttgccggt tatagtgccc catccgatag tacatatact
    10681 aagcaggtag agcaacttta aaaagagcgg caaggactta ccttgcttta ccagtttata
    10741 atgggcaaat atagctacca ggaaaaaaat caaaccataa ccggcatcgc cgataatcat
    10801 tgctacgaat agcgcaaaga aaattaaaaa atactggcta atatcatatt cccgataacc
    10861 gggcacagtg ccgaggaaat ctaaaaccgg ttggattagc cgagcaattt tattcatttt
    10921 taataaagtg ggcggattat cggtctcatc cggatctgca cttagaatac cccacccttc
    10981 gtcactagcc gcctgcatca attgggacac atcatccgag gggcaatatc cttgcaaata
    11041 ggtgatctct tctgcatcct gcattcccga tcggacgcgg gcgaattcca gatgcttttc
    11101 caattcctgc tgataggcgc gaattaaacc agccttttta gccagctcag taagttgctg
    11161 ttcgatttcg gctaatcggg aagtaaaatt ctgtaattcc cgtgtgaccg tgtcaaaatc
    11221 ttccggtggg atttgctcct ctttgagatc aagacgtaca tcctcagatc gggcaataaa
    11281 agctattagt agttgatttt tttcctcttt taatatttcg acggccgctt cttccggtat
    11341 atttttcaat atactacgat caattacata taaatgcagg tatatcctgt gttcttttag
    11401 ttcagccaca tctttttcgg aaattatgcc ccagcggtta taccagttta agtagatgtt
    11461 tttctcatca attttcgatt tcagattttg tttttcggtt tcgagagcga taatatcgga
    11521 aaccaatttc tgacactttt ctaaatcgac attttctgac gacggcggtg tattatagcc
    11581 ctcgataatg cccagacttt tctcgacagc atatatggca ttctcaattt ttgaggtatc
    11641 ctctgaaatc ggcggcttga tatgctggat gtgaaccact cctaatttgc gtaatttctt
    11701 cagagcggaa tctcgttccg gagtatgcac aaaaagcact acttttttca tcggaactat
    11761 cataccttta acgcctctct tttactctga atcttagatt tagcaatctt accgcgcact
    11821 acttccgccg tctgcgcatc gccgagaaag atctggatta tacggatgtt ttcccgagtt
    11881 tcgggaattt taactttctc gaacaagtta atgcgttgaa tggtgacgcg taattcttgt
    11941 tgtagaactt cgatctgttc ctgtaaaatt ttaattttaa tccggcgtgc aatttgctct
    12001 ttaaggatgc tgagagcgcg atcaacccac agaggcgttg taaaaaaatc atagggcaca
    12061 tcttcaaatt caacctggcg gaaaatccgg atttcgattc cggcaatatt gccttcgtca
    12121 gtgtggattt ctttgatttt caaccaggct tttaaatcta cctcttcagc cataacgtca
    12181 acccaggtaa acacctgttc agtaactttc cggatttctt cctcaacctg ctcgatagcg
    12241 agactaagat gccggatttc agtaaccagt tggcttttct ttaactcgag agtcgggaga
    12301 aagcgctcgt agcgtgctaa ggcttcgcgc tgagctttta attcattttt ggtatgttta
    12361 atcttagcca taattattta tatgaatcaa gcgctcactt ctgctttctt tggccagaat
    12421 ttctgcacca attctgattt cattcctgtc tcctgaggtt cgaaacaatc tgccagaatt
    12481 tgccagccga ggtttagagc tgattcgagc ggaatattaa ccttcaaatc catcaaatcg
    12541 ttctcaaacc gctcgccgta tttcagcaac ttgcgatccc aatcactcat ccggaatccc
    12601 atcgagcgct tctcctcagt ttccttgaac tgagcgtaaa gctggatcat accgtccata
    12661 atggcgcgat gatcttcccg cgtactttta ttgacttgtt gttttagacg actgagtgaa
    12721 ccaaaaggct cgatgcgacc gttgcgcaag taaaattgtc cttcagtaat ataaccggtg
    12781 ttgtccggaa cgggatgggt aacatcgtcg cccggcatag tcgtaaccgc cagaatggtc
    12841 accgaaccgg cgccttcgaa atcaactgct ttttcgtaac gcgcggctaa ctgactatag
    12901 agatcgccgg gataaccgcg attggatgga acctgttcca tcgtaatggc aatttccttg
    12961 agcgcatcgg caaaattagt catatcggtc agcaaaacca gcacatcacg tcctttgagt
    13021 gcaaagcgtt ccgccacggc taaactcaaa tccggcacca ttaaaccctc tacgattgga
    13081 tcggaagccg tgtgaatgaa aaatatcgag cggcttaagg caccaccttc ttcgattgta
    13141 tttttaaaaa acatatactg atcatatttc agaccgatgc cgcccagtat aatcatatca
    13201 acttccgcct gtaatgcaat gcgcgccaaa agctcgttgt atggttcacc ggaaaccgag
    13261 aaaatcggca atttttgcga aataaccaga gaattgaaca catcaatcat tgggatattg
    13321 gttctgacca ttcgcctggg tatgatgcgc ttagccgggt tgaccgaagg accagcaatc
    13381 tggatcatat tttctgttaa ttgtggacct ttgtcgcgcg gcacaccact gccattgaaa
    13441 atgcgaccga gcatattatc cgagaaagta acctgcatcg gatgccccag aaaacggatt
    13501 tcatcgttgg tggcgatgcc ctgactgcca gcgaatacct ggagcgaaac aatatcgcca
    13561 tccagtttaa tgacttgcgc cagagattta ccgcgctggg tagaaatttc cgccagctct
    13621 tcgttagcta cgttgctggc ttttactgtt attacgttgc cggttacgct taaaatttta
    13681 ttatagactt tttgcatcgc gggctttttc cttgatcaat tgatttattt tctgttcttg
    13741 tattttgaat tcatcgtcct taaaatcaat ataattccag tcaatgaaga cttgccgcaa
    13801 tttattgaaa aaatcgcggg cgtcgttttt atcagtcaga ttaaatttgg tcatcaatac
    13861 ctcatgcacc ttgctgaaga catatttctg gcgctcgggg ctactggcgc catctacctg
    13921 atcgaaagaa ttctgctgta agtaaactgc atccagaaac tcagatttca tataaatttc
    13981 aaaatcatct aaggaagtgc cttcctcacc gactaccagc atcatctggt gcacttcatt
    14041 acccttgaaa agtatttggc gcgcatcctc gattaaattc gagggaatga aactggtgta
    14101 tttcgaccag ctttccaggg gatgaatagc cggataacgg cgggcattgg agcgatcgcg
    14161 ggaaagcccg tgaaaagcgc cgacaacttt caaggttgat tgagttaccg gctcttcgaa
    14221 attaccgcct gccgggctga cggtacctcc gatggttaaa ctaccacgac taccatcgag
    14281 caactcgaca attccggcgc gttcgtaaaa ctgggcgatg cgcgattcaa gataagccgg
    14341 gaaggcttct tctcccggaa tttcttccag acgaccggat agttcccgca ttgcctgcgc
    14401 ccaacgtgaa gtggaatccg ccagtagtaa aacattcaat cccatctggc gataatattc
    14461 accaatcgtt gcagccgtat aaaccgaggc gtcgcgcgcg gcaaccggca ttgaactggt
    14521 attgcaaata attatggtgc gatccattag ggagcgaccg gtgcgcggat cggtcagttc
    14581 aggaaattcg cgcagagttt caactacttc accggcacgt tcaccgcaag ccgcgatgat
    14641 tacgacatcg gcttcggcaa agcgactggt cacctgttga agtaccgtct taccggcgcc
    14701 gaaaggtcct ggaatacaat aggtaccgcc tttggaaatc gggaagaaag tatcaataat
    14761 tctggtctgc gtcaccagcg gttcagtcgg tacaaatttc tcacgatagg ctgttattgg
    14821 aatcttgact ggccattcga agttcatttt caaattgatt tcgtcacctt tttcattggt
    14881 aattacggca atagtctcgg ttattttata gttgccttcc ggcacaatcg ttttgaccgt
    14941 atattcacca cgcaggttga aaggcacaaa aatttgatgc tcaaaaatac cttccgagac
    15001 attgccgagg ctgataccag cctggacaat atcgccggac ttgaccagcg gtttaaaatc
    15061 ccattccttc tttgaatcca gcggttcgat aacagctccg cgctttagga aaaagccgaa
    15121 tttagccgcc aagtcgtgca aaggattctg gagcccgtca taaaccatac cgagcaagcc
    15181 gggaccgagc cgcaccgaaa gcatttgtcc gctgaattcg accttatcgc caactctcag
    15241 acctttggtg ctttcgaaga cctgcatata agccaggttg ccttgaatcc ggatcacctc
    15301 tgctttgaga ttctcatcgc caaccacagc gtaaccgact tcattctgga taatgtcgct
    15361 ctcacaagcg acggtgatca tattgccgtt aatgctagta atttttccgt attttttcat
    15421 aattccattt ctaaatattg ttgatatttt tcacgtccga tttttttatt gaaagtaaat
    15481 agtcttttta aaatctggag tcgtagataa tacataatga gaaattccag atcgaaataa
    15541 tggccgaatt ccagcgaatc taaaaattgc cagcgccata gtaagagatt cttttcaacg
    15601 gttaagggat cgccctctcg cactaaatta gcaggaaacg ctataatttt ataatcctga
    15661 ttcttcttct gggctgtccg ccattcaacc agttcgaggc gtaaatctaa ttcaaaattt
    15721 ttgattcgac gaataatatc gggatcgttt ttatccggcg ctgtggcatt caaatccacc
    15781 ttttccaatt gcgcataatc ctcagccgaa agccatttgt aggcttcggt taaaaattgt
    15841 tcgatggtca gcgccggttc tcgctcaaaa tataaaacag gcaactgagc gattaaatag
    15901 taatatttat ccattctgat ctaataattg gcgcagagcc ggattgagaa attcctgtaa
    15961 aacttcagcg atgctttggt cggttagatc ataatagacc tcttcacctt tgcgcgaaac
    16021 ccgaaagccg gcggcaatac cacggtcaac tcggaaagta accggctcct tgagactatc
    16081 gcgagtcgcc gccaatacca aagtcttcaa tttctcaata tctttttcgg ctaccacgaa
    16141 ttcatactgg ttgtcggaag atgtagattt taccaatgcc agaatcatct cttttaaaaa
    16201 ttccggcgtc aatgttttgg ctatttcttt cttaaaaacc cgatcgaaaa gtttcaggag
    16261 attttcttta gttacgagga ttgtatcacg ggcggcttgg cgaattgcgg cttccgcgtt
    16321 agcgcgcaac ttttcggcat tctgttgacc ttcattgata atctggcggg ctttttcatt
    16381 agccttattg ataatttcct gagcttcggc ttcggctttc tggcggattt catcggcggc
    16441 gcgctgagct tcttcgatgc cttcctgctt tatcttctct attaaactgt ccagtttaac
    16501 gtccataaaa atctcctgta gttcggcttc ttattcatta gaaaataact tgtgaattta
    16561 acttctatga attgttaagt caagcaaggt tgaagatttt ttggataaca tcaagacaga
    16621 atatttacca tttgaatata aataggatta atcccaaacg atttcaaaaa acccttactt
    16681 cgtaagcaag aaatataatt cctgcttttt ggatgattaa aaataataag cgagattgat
    16741 ccggtgctgt tggcccccac ggtaggtgta atccacgccg ggcacttttt tagacaaacg
    16801 gatcgggcta tcatccattc tgcgaccgga cggtattagc tgctgaaaag tataggcaat
    16861 tccgagcggg cctcggcgcc attctgcttc ggcatttaaa tcgaataaat ggagactata
    16921 tttgtatgga taatccagcg gatccgattt aatacccatc atcaatttgg ctttcccttc
    16981 aagccagaaa tcgaataaag tatgagaata taaagcctta gcatgaaagt taatattctg
    17041 ccagttgaaa tttgtcccaa aaccaatctg ctgactaaag gaatggccat acgaaaaatt
    17101 caattccgcc gaatgaaaga tcggcaatat tcccaactgg aatccgagta cgggtgtact
    17161 cagaaaaata tcagactcgt aattagcata agatagacca cctgatattt cgaacaactt
    17221 ttcacccaat gtaaactgaa ttgcataacc ttcgcgatcg atatacccct gcccgagcga
    17281 atccagatca gtaaaattcg ggggattgtt atctatgaaa taatcgaagt cattgcgtaa
    17341 aaacatcacg gaaatatctt taacaaaact ttgatctgca ttcaaagcaa gtcggtaata
    17401 atgatttaat ccattaattg gaaggtcaat ttgccgttga ccggctaaag ccgcctcttg
    17461 accggtgttg atataacgaa tacgccagtg gaaatcaaaa tcagttgccc gaattgccaa
    17521 accgagatga taattgctat tcagaggaag tgaagtccat agtgaataac cgatttgatt
    17581 aacgtgcaag gtgttagtca attcctctcc aaaagtcggt tccagcaagt cgaaaaagta
    17641 tttattagta agttcatctt cggaatgtgg gaacgagata atattaatta aaaagggggc
    17701 tgagttccgg ttatattcca agccgccacc gaagtgaata cgttcgtaag ccattgccca
    17761 taaagtcgaa aaataagtat tatcaatttg acaatgactg accagactgg tctgatcgct
    17821 gaggttatca aaattagaaa aatttcttgc cgcctcggct ttgattagat gagatgtttg
    17881 ccttagacga aaaggcaaat tgagttgcaa ggtattcgtc tgttcggaga tggtcaaagt
    17941 cgtctgttga tcaacctttt tgcgataaat atcatcataa ccgattcggg ctttaataaa
    18001 aggcaaactc ggtgaattca gttcggcaag tgttacggtt ggaattttag gaaatgccag
    18061 caaattgtaa agtgaagcct gccaatggct ggcaaatgcc aaattggaca gactgacagt
    18121 caaaaaaaaa gtggggaaaa ggtagtggct tttccccaca aaaaactgat ggatcagtcg
    18181 caactctggt cctattttag cagagtcatt ttgcggatgc tccggtgacc attgacttct
    18241 atcatagcaa aatagatccc ggatggataa tgggaagcgt tccagtcgta ggaaatccga
    18301 ccagccgata tcctgcgatt gacgatctgt tcgattactc gtccctgcag atcaacaatg
    18361 cacagtttga catcggcggc ttgcggcaaa tcaaatgtga tatttgccac cggattaaag
    18421 ggattaggat aaactggata aagcttgaat tcggtcggta aagcttgatt ttcctgcgcg
    18481 atgccgactt taaaccggtc gggaaacatt ccgcccaggg tgctgtcggt gcccataaag
    18541 aaattcttca tcataaccag gaaacttgtc ggcggattat cgaaccaggc tgaaacattg
    18601 actcgctc
//
LOCUS       DAOOMU010000041        18545 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_3069_length_18545_cov_13.818929, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000041 DAOOMU010000000
VERSION     DAOOMU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 18545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18545
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3069_length_18545_cov_13.818929"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLI55_05470"
     CDS             complement(<1..195)
                     /locus_tag="PLI55_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00317.1"
                     /translation="MSAYRFRLAIPFLGLLFLSGWAQIPCADFEIVESIPIETILDNP
                     EIRNTSEVWAEMIGAADETID"
     gene            complement(215..874)
                     /gene="plsY"
                     /locus_tag="PLI55_05475"
     CDS             complement(215..874)
                     /gene="plsY"
                     /locus_tag="PLI55_05475"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPB00318.1"
                     /translation="MITLLAIIVFSYLIGSIPTAIIVGKITKKIDIRQYGSCNAGATN
                     AFRVLGWKAGLFVALFDIAKGAFTTAYISQIRFDIPPLNPTWLMILAGLCAILGHTFT
                     IFAGFKGGKGVAVGAGMIIALYPLALLPCLLVFSIVLFSTGIVSVSSISAALSLPIIL
                     LLFNYFGTSPIDKALLIFSIFIPIFIVYTHRANIRRLFKGEEKPFEKLILLRRRRSSK
                     K"
     gene            complement(874..2658)
                     /locus_tag="PLI55_05480"
     CDS             complement(874..2658)
                     /locus_tag="PLI55_05480"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPB00319.1"
                     /translation="MKIKYIDGKRFIRGIKVGAYKIVNHQDYLNKINVFPVPDADTGT
                     NMAATMKTIVDNIQEDTNQIDEATKNIANSALEGARGNSGVILAQFFYGLAKGIGKNK
                     RLTTIQFGEAVNSAKSHAYEALSKPREGTILSVIKDWAENIYENSKKIFDFQELLNHS
                     LEVARHSLEKTRTKIEAMRNANVVDSGAQGFVYILEGISNFINRGRLKELEKNCIQPV
                     SVIARTEVLPEQITYRFCTECLIEGKNIPIENLRLEFEPYGDSIVLAGSPQKARLHIH
                     TNEPAKVFKIAHQYGDLLQQKADDMLKQYLIAHNPHPKIALVVDSACDLPPEFIDRSF
                     IHIIPIRLTFGQSSYLDKVTITPEYFYEMLDTEPEHPRTSQPSPADFINLYSFLLSHY
                     DSIISIHLPATLSGTYQNALNAAQNFPERKISVIDSKALSVSFGFLAEQAVELIAAGK
                     SHNEIVSELEKATRRTQLFVGVPSLKYLMRSGRVSKAKGLIAKLFNIKPILNLDEYGS
                     PQHISKSFSKKGAFTKVLQMASNFAAQKINPRFAIAHANNLKIAEYFNNELKKRFKVE
                     KINILPVAPMLGAHAGNGAAAIAITWSE"
     assembly_gap    2686..2785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2786..3225)
                     /locus_tag="PLI55_05485"
     CDS             complement(<2786..3225)
                     /locus_tag="PLI55_05485"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HPB00320.1"
                     /translation="MPKDTANPKSNWWQKWKTSQRNKIIAGIIALIPVVLTIYFIKLV
                     ISFFDRIVSPVLDPLIGFHLPGLGLIVALIVIYLLGLFITNFIGKSLILLIEKWLNYI
                     PIVRTIYNTSKQVMNALSFSKVGFEKTVIVEYPRKNTWAIGFLTG"
     gene            complement(3252..3728)
                     /locus_tag="PLI55_05490"
     CDS             complement(3252..3728)
                     /locus_tag="PLI55_05490"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPB00321.1"
                     /translation="MTNQSSHPNWIIGLFLILLGLLIFLIQFDYFDWSDFWPLIIIGV
                     GLLFLIGFFVNRNNYGLLMPAIILLIVGTLFLYLERTTWTNMNYLWPIFILAPGVGFF
                     FMYFFGPQGNNLWIPGTILIVISVIFLAGEWTILRYWPVILILLGIYLIFSKKPSS"
     gene            complement(3827..4876)
                     /locus_tag="PLI55_05495"
     CDS             complement(3827..4876)
                     /locus_tag="PLI55_05495"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HPB00322.1"
                     /translation="MSKIEEYLGKDADFLLNHKCMTISKELLHLPGPDFVDRILLYSD
                     RPVGVLRNLQTMYNTGRLSGTGYLSILPVDQGIEHSAGASFAPNPMYFDPENIVKLAL
                     EGGCNAVASTLGVLGSVARKYAHKIPFLLKLNHNELLSYPNMYDQTMFASIDQAFDMG
                     AVAVGATIYFGSAESRRQIMEVSEAFEYAHQLGMVTVLWCYLRNNAFKTAEKDYHVAA
                     DLTNQANHLGVTIEADIIKQKLPECNGGFLALKFGKTNKKVYEQLTTDHPIDLTRYQV
                     AGCYMGRAGLINSGGASGEDDLTEAVKTAVINKRAGGMGLILGRKAFQRPMAEGVKIV
                     NAVQDVYLDKNVTIA"
     assembly_gap    4894..4993
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5051..5473
                     /locus_tag="PLI55_05500"
     CDS             5051..5473
                     /locus_tag="PLI55_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPB00323.1"
                     /translation="MINIKGELEKCGIKPTFQRLCVLELMEQSSEHLTADAIYESLLK
                     KMPTMSRTTIYNTLNLFLEKGLIAPIMITGTEVRYEISTNQHHHFLCLQCNRIYDVDV
                     QCPLFESKEVDGHKIQEIHGYFKGICRNCLKQIEREPK"
     gene            5470..5694
                     /locus_tag="PLI55_05505"
     CDS             5470..5694
                     /locus_tag="PLI55_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPB00324.1"
                     /translation="MKEKVEEVIESIRPMLMADGGNVELVEVTKDGVVRLRLTGACGG
                     CPMSTYTLRMGIERRLKEAIPEIKSVEQVF"
     gene            5759..5962
                     /locus_tag="PLI55_05510"
     CDS             5759..5962
                     /locus_tag="PLI55_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HPB00325.1"
                     /translation="MKYTFKIPTMSCQHCKMRIEKALSPYVNPANIAFDLTSKKVAVE
                     TDIPLTILIEAIESVGYPVERDN"
     gene            5983..6558
                     /locus_tag="PLI55_05515"
     CDS             5983..6558
                     /locus_tag="PLI55_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPB00326.1"
                     /translation="MPSLKGTRTEKNLLTAFAGESQARNRYNYFASQAKKEGYEQIAA
                     VFAETADNEKEHAKRFFKFLEGGEISITAAFPAGVIGTTAENLLEAANGEKYENSVMY
                     PEFAKVADEEGFTEIANVFRAVIVAETQHEKNYRALRKNVLENRVFEKEQVVTWRCRN
                     CGYIHVGTKAPEKCPACAHPRAYFELLAENY"
     gene            6596..6760
                     /locus_tag="PLI55_05520"
     CDS             6596..6760
                     /locus_tag="PLI55_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPB00327.1"
                     /translation="MTKYVCTICGYVYDPRKGDPDNGVPRGTAFEDLPDDWVCPQCGA
                     EKEDFEPQED"
     gene            6763..7944
                     /locus_tag="PLI55_05525"
     CDS             6763..7944
                     /locus_tag="PLI55_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPB00328.1"
                     /translation="MAVQILKPDIFSVGARDWDRRLFDELIPLPDGTTYNAYLIKGSQ
                     KTALLDTVDPPKKHELLENLQEIGIERIDYLISHHAEQDHSGTIPAVLEQFPMAQVVT
                     NQKCRALLQEHLLIPDEKFKIINDGEELSLGNKTLRFIFTPWVHWPETISTYLEEDKI
                     LFSCDFFGSHLATSNLFVDDESRVYRSAKRYYAEIMMPFRSNVRQNLEKVKNLAIEMI
                     APSHGPIYPNPEFIMEAYADWTSDSVKNIVLIPYVSMHGSTEAMVQYLTKALIEREIT
                     VQPFNLSRTDIGELAMAVVDAATVIIGSPTVILGPHPAAVSAAYLVNLLRPKTRYLTI
                     IGSFGWGGKMPEQIKALLSGMKAEFLSPVIIKGYPKLEDFSALDRLADEIAARHRALS
                     S"
     assembly_gap    7969..8068
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8069..8305
                     /locus_tag="PLI55_05530"
     CDS             <8069..8305
                     /locus_tag="PLI55_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00329.1"
                     /translation="YTQNLIFSDANDETKIPAINDAKTALLYAIERELDSVLYYKEIK
                     ELIPASEHQLISGIIDEERRHVVRLSELKKNLTF"
     gene            8425..9516
                     /locus_tag="PLI55_05535"
     CDS             8425..9516
                     /locus_tag="PLI55_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB00330.1"
                     /translation="MKFKNEYPRRVLTARQRKRPEDIRGPFFRDQTAIIHSLAFRRLK
                     HKTQVFFSPENDHICTRIEHSLHVATISATICRALKLDIDLATAIGLGHDLGHAPFGH
                     AGEKWLENLFGGFVHELHSLRVVDKLENYGQGLNLTYAVRDGIVSHCGEVDEQFIEIS
                     PTLNDLDNLQFRPVQPSTWEACVVRLSDSIAYLGRDLEDAVAANLINKEDLPEKLRRG
                     IGQSNSEIINNLVFDVIDWSTKNGKIGFSPEKFALISEFKEFSREKIYDNPKLKYWRS
                     YSGFVIETLKNYLIDLYEKYQNDYERYANSRTPLDVKFGKYLKSLHERYQAEKCSSEL
                     IVRDYIAGMTDAYALHCFQNILTMGTRGL"
     gene            9802..11007
                     /locus_tag="PLI55_05540"
     CDS             9802..11007
                     /locus_tag="PLI55_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HPB00331.1"
                     /translation="MKKNGWLVIFLLCVPLMAQKFDKMALTPPMGWNSWNRFGCDVNE
                     QLIRETADAMVSSGMKAAGYQYVVIDDCWQGERDALGFIQPDPGRFPSGMKALADYIH
                     SKGLKFGIYSDAGWKTCGGHPGSRGYEYQDALTYAKWGVDYLKYDWCNTEGLNAEGAY
                     LTMRDALFAAGRPIVFSICEWGDNRPWEWGKDIGHLWRTTGDITAIFDGQEGHGTWNS
                     WGVSQILDMRENIRQYAGPDHWNDPDMLEVGNGMTIAEDRAHFSLWCIQAAPLIAGND
                     LRDMSSEVIDILTNKELIAIDQDPLGIQGFKYLVKDNWEVWAKPLDKGDLALCFLNRT
                     LEPVKVDLDWKKYPVKDDEFHYYYLFNENEYKIRDLWLKKEIGTTAKPFSTQIGAHDV
                     IVLRLSPVK"
     gene            complement(11010..11717)
                     /locus_tag="PLI55_05545"
     CDS             complement(11010..11717)
                     /locus_tag="PLI55_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00332.1"
                     /translation="MNNNTEIIDLKKEQISSLERLHYKYSLMARAFFVLMDIVTGYQI
                     TLSKAKLIEILASIPYRAWEVRHYSRLTRVYRNYEKVTRSQRIVTWSRSAQDNEYWHL
                     LVIHEKMKTDGIRDAWYLFPLVPYVMVAVYRLFAKTLAFCNIRRAYLFNAEFEDHAEH
                     VYAQFVADHPEWETQPVESDLVNEYGKFSSWADVFRRIGLDERDHRNHSFLLCGKPEA
                     VVSYNGMPKDINFPTGR"
     gene            complement(11891..12232)
                     /locus_tag="PLI55_05550"
     CDS             complement(11891..12232)
                     /locus_tag="PLI55_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPB00333.1"
                     /translation="MNIYVGNLAPNVTEGDLRVAFEAFGQVASASVIKDKFTGESRGF
                     GFVEMPLMAEAQAAISNLNGKDLKGKTITVNEARPRTEGGRGGDRFGGHSGSGHFGGK
                     PRDNRRGGGRY"
     gene            complement(12565..13605)
                     /locus_tag="PLI55_05555"
     CDS             complement(12565..13605)
                     /locus_tag="PLI55_05555"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPB00334.1"
                     /translation="MGHSQVQTILWPELNGTALWDSVIANFKPIKTLGYDKSRDTMYA
                     VIDLKNGGSLSCVYTGYTIKLDRTKDPSTDAYNKSIDCEHTWPQSMGADSEPQRSNLH
                     HLYPTFTNVNSSRGNDPFAEIPDAKTDKWWYLNKSQTIIPTSNIDKYSEKENDPPACF
                     EPREDHKGNCARSMFYFYAMYSEVYFTKDPDMTFWNFQKETLLAWNYYDTVDANEYAR
                     TWKIATYQDNKPNPFVLDSTLARRIWFYKPSTLVIEKPNLTGDFKIHPCYPNPFNNTT
                     NLAFTLPEIENVTLMVFDLNGHLVNEQQLGRFPSGHHIVPWSGSNLSSGVYIYCLQAG
                     QRAHAGKLLIIK"
     gene            complement(13685..15769)
                     /gene="fusA"
                     /locus_tag="PLI55_05560"
     CDS             complement(13685..15769)
                     /gene="fusA"
                     /locus_tag="PLI55_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB00335.1"
                     /translation="MDNIEIQKIRNIGIVAHIDAGKTTTSERILFFTGMTHKIGEVHN
                     GQAVMDFMKQEQERGITITSAAITTEWKDHQINLIDTPGHIDFTVEVERSLRVLDGIV
                     MVFCAVGGVEPQSETVWHQADNYKVPRIAFINKMDRAGADYFQTINMMNEMLGANAVA
                     FQLPIGNEADFNGIIDLITMKAYSYRELDMFESEIPSNLLPKAQKLREQMIEKLADFD
                     ENLMEKFINGMAISESDIRNAARYAVLNLCVTPVFCGSAFKNKGIRLLLDAIVDYLPS
                     PVDRGIITGRDANNPEVIISRKPHISEPLTALAFKITNDAYVGQQTFVRVYSGELHSG
                     SYIFNSTKQKRERVGRVLRIRADERTEITSLKAGDIGALIGMKLTTTGDTLCNENDTI
                     LLENIRYPETVLDMKIEPLAIKERDRLGIALSRLALEDPSFKVRFADETEETIISGMG
                     ELHLEIIVDRLKNEHKLDVNVGKPAVAFRETITREAEHTYRYKKQTGGKGQFAQIAFR
                     LEPNPGKGIEFVDRVKGGNIPREFIPAVEKGFREIVEKGLLAGYPMVDIKFVLFDGAY
                     HPVDSSEMAFRMCTILGMKEIINKTAPQLLEPIMKIEINTPDEYMGDAIGDINRRRGK
                     IENMRRHRKGSQKLNGTVPLMEMFGYASALRTISSGRAAFSMEFLHYTPLPKSIEESI
                     IEERRKKKAERD"
     gene            15933..16325
                     /locus_tag="PLI55_05565"
     CDS             15933..16325
                     /locus_tag="PLI55_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00336.1"
                     /translation="MVSGLIFGGLYSFLVVCQVGGKESDLVAPVSIELDATMVSGLEA
                     EVDHFKIKSSIKYSQVVQMVEKRRRLMDKVAYGSVSDSETFGENFYAFCRDNGLDLRR
                     RVLLLSYGADGLTVWRRTYLWRQREGWI"
     gene            complement(16368..16604)
                     /locus_tag="PLI55_05570"
     CDS             complement(16368..16604)
                     /locus_tag="PLI55_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00337.1"
                     /translation="MAPRHTLFFKTVTNQDADALFHAARTMTFAGQIMDLFPVIELVS
                     HQVIHGFTIETFPLANFLDISNYLFATRKPYGLT"
     gene            16869..17591
                     /locus_tag="PLI55_05575"
     CDS             16869..17591
                     /locus_tag="PLI55_05575"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPB00338.1"
                     /translation="MQNLFPKIGTDLTLSQEIVKKLEDIIRQKKVLPGEKLPSEMEMC
                     SMFGVSRTSLREALQMLSARGLITIRKGSGIYVNNYSPAHAIKPMSLFLELNLDREYI
                     THVMEVRKMFEPNIASLAALNRTETDIQKLEQNLEELKNSPIDNFYKQGEIDRDFHLI
                     IAEACKNSIVQIIVTPIFELMPKIRSIVYAYVQTAKDEAIDFHFKIFEKIKEKDADGA
                     YQMMIKHLEIAEQHSRKVLDEI"
     gene            17659..18423
                     /locus_tag="PLI55_05580"
     CDS             17659..18423
                     /locus_tag="PLI55_05580"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPB00339.1"
                     /translation="MNINLTRKTAIITGSVQGIGFDIAKIFTECGASVVINNNLDADS
                     LEIKAEELRKSGADVKAVVADVTIKEEAQKLIDAALSFGDTIDILVNNARGLIKRVPI
                     AEFDENHFWTVVNVNLKSAFIMSHLVMPYMKEQRSGKIVNLSSQAAHDGGGSGAAVYA
                     ASKGGIWTFTKSLAKEVASFGICVNCIAPGFIENTAFHNTFSSKETHEKVLATVPLGR
                     LGTVRDISRVALFLASELSDYMTGQSLQVNGGLYMP"
BASE COUNT         5419 a         3482 c         4036 g         5308 t
ORIGIN      
        1 gtcaatagtc tcatcagccg cgccgatcat ttctgcccag acttcggaag tgttacgaat
       61 ttcgggatta tcgagaatag tctcaatcgg aatgctttcg acaatttcaa agtcggcaca
      121 cgggatttga gcccaacccg agaggaataa cagccccaaa aacggaattg cgagacggaa
      181 ccgataggct gacaaattca tcctaaacaa aaagttactt tttagaagat cgccgccgac
      241 gtaggagaat taatttttcg aatggctttt cttcaccttt aaaaagtcgc cgtatattag
      301 cacgatgagt ataaacaata aaaattggga taaaaatact gaaaattaga agtgctttat
      361 ctatcggcga agtcccgaaa taattgaaaa gcagtaaaat gatcggcaaa ctgagggcgg
      421 cgctgattga cgagactgat acgataccgg tcgagaatag gacaatgcta aagaccagca
      481 aacatggtaa aagcgcaaga ggatataaag cgataatcat cccggctccg accgcaacac
      541 ccttgccacc tttaaaaccg gcaaagatgg tgaaagtatg gccaagaatt gcacataaac
      601 cggctaaaat cattaaccag gtgggattta acggcggaat atcgaagcgg atttgggaaa
      661 tgtaggcggt agtaaatgcc cctttggcaa tgtcaaaaag agccacgaaa agcccggctt
      721 tccagcctag gacgcgaaac gcattcgtgg cgccggcgtt gcacgaaccg tattggcgaa
      781 tatcaatttt tttagtgatt tttccgacga tgatggcagt gggaatcgaa ccgattaaat
      841 aactgaatac gataattgcc aataatgtta tcatcattca ctccaggtaa tagcgatcgc
      901 cgccgcaccg tttccggcat gcgcgcctaa catcggcgca acaggtaaga tattgatttt
      961 ttcaaccttg aaacgttttt ttaattcatt attgaagtat tcagcaatct tcagattgtt
     1021 ggcgtgagcg atagcaaacc gcggattgat tttttgagct gcaaaattgc tggccatctg
     1081 cagaacttta gtgaaagctc cctttttgga gaaacttttc gatatgtgtt gaggtgatcc
     1141 gtattcgtcc aaatttaaga tcggtttgat attgaataac ttggctatca agcctttagc
     1201 cttcgagact cgcccgctac gcattagata tttcaaactt ggtactccga caaataattg
     1261 tgtccgacga gtagcttttt ccaattcact gacgatttcg ttatgagatt taccagcggc
     1321 aattaattcc accgcctgtt cggcaagaaa accaaaagaa accgaaagag ccttactatc
     1381 aatcacgcta attttccgtt cgggaaaatt ttgggcggcg tttagggcat tctggtaggt
     1441 accgctaaga gtggctggca ggtgaattga aataatcgaa tcatagtggc tgagcaagaa
     1501 actatataaa ttgataaaat cagccggtga cggctgagaa gtacggggat gttccggctc
     1561 cgtgtcgagc atttcataaa aatattcggg agtgattgtg actttatcaa ggtaactcga
     1621 ttgaccaaac gtcaatcgta tcggtataat atgaataaag gagcggtcga tgaattcagg
     1681 tggcaaatcg caggctgaat ctactaccaa cgcaattttc ggatgtggat tatgggcaat
     1741 taaatactgc ttaagcatat cgtcggcttt ttgctgaagg agatcgccat attgatgggc
     1801 aattttaaaa actttagccg gttcgttagt atgaatatgc aaacgtgcct tctgcggtga
     1861 accggctaag acaatcgaat cgccgtaggg ttcgaactcc agacgtaggt tttctatcgg
     1921 aatatttttc ccttcaataa ggcattcggt acaaaaacgg taggtaatct gttccggtag
     1981 tacttccgtg cgggcgatga ctgagaccgg ctgaatacaa tttttttcca gctccttgag
     2041 tcttccacga ttgataaaat tactgattcc ttccagaata taaacgaatc cctgtgcgcc
     2101 ggagtcaacg acgttggcat ttcgcatcgc ctcaattttg gtgcgggttt tttccaaaga
     2161 atgtcgggca acttctaggg aatgatttag taactcctga aaatcgaaaa ttttcttgct
     2221 gttttcgtaa atattttcag cccaatcttt gatgacagat aggatggtac cttctcgcgg
     2281 tttggatagc gcttcgtaag catgactttt ggcagaattg acagcctcgc caaattgaat
     2341 ggttgtaaga cgtttatttt ttccgattcc ctttgccaat ccataaaaaa actgagccaa
     2401 aataacgccg ctattaccac gcgctccttc gagtgctgaa ttggcgatgt ttttcgtcgc
     2461 ttcatcaatc tggtttgtgt cttcctggat attgtccact atggttttca tagtggcggc
     2521 catattggtg cctgtatctg cgtcaggaac gggaaagaca ttgattttgt tgagataatc
     2581 ctgatggtta acgattttat acgcaccgac tttgatgcca cgtataaatc tcttaccatc
     2641 aatgtattta attttcataa atgatggtct cttctctatt ttttannnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnncctgt taagaatcca attgcccagg tatttttacg
     2821 gggatattcg acgataacgg tcttttcaaa accgactttg gaaaaggaaa gagcattcat
     2881 tacttgtttg ctcgtattat aaatcgtgcg aacgatggga atgtaattaa gccatttctc
     2941 aattaggaga attaatgatt ttccaatgaa attagtaata aaaagaccaa gcaaatagat
     3001 gactataaga gccacgatta aacccaaccc gggcaagtgg aaaccaatca gaggatcaag
     3061 taccgggcta acgatccgat cgaaaaaaga aataactagc ttaataaaat aaattgtcaa
     3121 tacaactgga attagggcaa ttattccggc aattatttta ttgcgttgag aagttttcca
     3181 cttttgccac caattggact taggattggc tgtatctttt ggcatttggg aattttcggg
     3241 agaaggtggg ttcatgaaga aggtttttta ctaaaaatta gataaatacc aagtagaatt
     3301 aatataactg gccaatagcg taaaatagtc cattcaccag ccagaaaaat aacggaaatc
     3361 acgattaata tagttccggg aatccagaga ttattgcctt gtggtccgaa aaaatacata
     3421 aagaaaaaac caactcccgg tgctaatata aaaatcggcc agaggtaatt catattagtc
     3481 caagtggttc tttccaaata aagaaacaaa gtcccgacaa ttaaaagaat gatagcgggc
     3541 atcaatagcc cataattatt gcggttgacg aaaaaaccta ttagaaataa taatcctact
     3601 ccgataatga tcagcggcca aaaatcagac cagtcaaagt aatcgaactg aattaagaat
     3661 atcagcaaac ccagcagaat taaaaatagt ccgattatcc aattgggatg cgaagattga
     3721 ttagtcattt cctacctcga aaaaggtaaa gccccgaacg gggctttacc atcttgattt
     3781 tattatccaa ggatagcctc ggtatagatt gccccattga ttcaggttag gcaatcgtaa
     3841 catttttgtc gagataaaca tcctggacgg catttacgat cttgacgcct tcagccattg
     3901 gtctctgaaa agctttgcgt cccagaatca aacccatacc gcccgcccgt ttattgataa
     3961 cggctgtttt gacagcttca gttaaatcgt cttcaccgct ggcgccaccg gaattaatca
     4021 atccagctcg tcccatatag caaccggcga cctgatagcg agtaaggtca atcggatgat
     4081 cagtagtcaa ctgttcgtaa actttcttat tagtttttcc gaactttaag gctaagaatc
     4141 caccattgca ttcgggtaat ttttgcttaa taatatcggc ttcaatggtc acaccgagat
     4201 gattagcctg attggttaaa tcagccgcga catgatagtc tttttcagca gtcttaaagg
     4261 cattgttacg taagtaacac catagtaccg taaccatacc aagctgatgc gcatattcga
     4321 atgcttcact gacttccata atctgacgac gtgattccgc cgaaccaaaa taaatcgtag
     4381 cacccacggc aactgcgccc atatcaaaag cttgatcaat actggcaaac atcgtctgat
     4441 catacatatt aggataggat agcagttcgt tgtgattaag tttcagcaga aagggaattt
     4501 tgtgggcgta tttacgggcg acgcttccca gaacgccgag cgtcgaagct acggcattac
     4561 atccgccttc gagggctaat ttgacaatat tttccggatc gaaatacatc gggttcgggg
     4621 caaatgaagc cccggcggaa tgctcgatgc cttggtcaac cggcagaatc gagagataac
     4681 cagtacctga caaacgaccg gtgttataca ttgtttgcaa attgcgcagg acgcctactg
     4741 gccgatccga ataaagcaaa atccggtcaa caaaatcagg cccaggtaaa tgcaatagtt
     4801 ccttggaaat tgtcatgcac ttatgattca gtagaaaatc cgcatctttt ccaagatatt
     4861 cttcaatttt actcatacaa aaacctccaa aaannnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 nnnnnnnnnn nnnatattac ttgaattttt ccataactgt tgttaatatt aacttgtaat
     5041 gagttcaaaa ttgataaata ttaaaggtga attggaaaag tgcggtatta aaccgacttt
     5101 ccagcgctta tgtgttttag aattaatgga acaaagtagt gagcatttga ctgccgatgc
     5161 gatatatgaa agtctgttaa aaaagatgcc aacgatgtcg cggacgacga tctataatac
     5221 cttgaattta ttcctggaaa aaggactaat agcgccgatt atgattaccg gcaccgaagt
     5281 ccgctatgaa atctcaacca atcaacacca tcattttctc tgcttacagt gcaaccgaat
     5341 ttatgatgtt gacgttcaat gtccgttatt tgaaagtaag gaagttgatg ggcataaaat
     5401 ccaggaaatt catggctatt tcaaaggaat atgtcgaaac tgtctaaagc aaatagaaag
     5461 ggaacctaaa tgaaagagaa agttgaagaa gtaatcgaat ccatccggcc gatgttgatg
     5521 gctgatggcg gtaatgtcga attagtcgag gttaccaaag atggagtcgt aagattaaga
     5581 ttaacaggtg cttgcggcgg ttgtccaatg agcacttata ctttgcggat gggcatcgag
     5641 cgccgtctca aagaagctat tccagaaatc aagagcgttg agcaggtttt ttagcagaaa
     5701 ataatctgat tttaaaatca cgattctatg attaatttta tcctagaaaa aatgatttat
     5761 gaaatatacg ttcaaaattc cgacgatgag ctgtcagcat tgtaaaatgc ggatcgagaa
     5821 agccttgagt ccgtatgtca atccggctaa tatcgctttt gatttaactt caaaaaaggt
     5881 agcagtggag actgacattc ccttgacgat attgattgaa gctatcgaat cggttggtta
     5941 tcccgttgaa cgtgataatt aataataaga aaggagaaat atatgccaag tttaaaagga
     6001 acccgcacgg aaaaaaactt gttgaccgct tttgccggtg aatctcaagc tcgtaatcgt
     6061 tataattatt tcgcttctca agccaaaaag gaaggctatg aacaaattgc cgcggtattt
     6121 gcagaaacgg ctgataatga gaaggaacat gccaagcgtt tcttcaaatt tttagaaggc
     6181 ggcgagatta gcataaccgc cgcatttcca gccggagtaa ttggcacgac agccgagaat
     6241 ttgctggaag ccgccaatgg tgaaaaatat gaaaattcgg taatgtatcc tgaatttgct
     6301 aaagtcgctg atgaggaagg gtttaccgaa attgccaatg tttttcgagc cgttattgtt
     6361 gctgaaaccc agcacgagaa aaattatcgt gctctgcgta aaaatgtgct ggaaaaccgc
     6421 gtttttgaaa aggagcaggt tgtgacatgg cgttgtcgaa actgcggtta tattcatgtg
     6481 ggtactaagg caccggagaa atgtccagcc tgcgcccacc ctcgagctta ctttgaacta
     6541 ttggctgaaa attattgatt aaataggatt tattaactca tttacaggag gtaatatgac
     6601 taaatatgtt tgtactattt gtggttatgt ttatgatcct cgcaaagggg atccggataa
     6661 tggtgtgccc cggggcactg cctttgagga tttaccggat gactgggttt gtccgcagtg
     6721 tggcgctgaa aaagaggatt ttgaaccaca ggaggactga aattggcagt ccagatttta
     6781 aagccggata tttttagcgt tggtgcccgc gattgggatc gccggttgtt tgatgagttg
     6841 atccctcttc cggacggcac gacttataac gcttatctaa tcaaaggttc gcaaaagacg
     6901 gctttgctcg ataccgtcga tccgcctaaa aaacatgaat tgctcgaaaa tctgcaggag
     6961 attggaatcg aacgcattga ttatctaatt tcgcatcatg cggaacaaga tcattccgga
     7021 actattccgg cggttttgga acaattccca atggcgcagg tagttactaa tcaaaaatgt
     7081 cgggctctac ttcaagagca tttgctaatt ccagacgaaa aattcaaaat tattaacgac
     7141 ggtgaagaac tcagcttagg gaataaaact ttacgtttta tttttactcc ctgggttcac
     7201 tggccggaaa ccatttcgac ctatctggaa gaagataaaa tcctgttctc ctgtgatttt
     7261 tttggttcgc acttagcaac gagtaattta tttgttgatg atgaaagtcg agtttatcgc
     7321 tccgctaaac gctattatgc cgaaattatg atgccgtttc gctcgaatgt gcgtcaaaat
     7381 ctggaaaaag taaagaatct tgccattgaa atgattgctc ccagccacgg accgatatac
     7441 ccaaatccgg aatttattat ggaagcttat gccgattgga cttcggattc ggttaaaaat
     7501 atagtgctta taccgtatgt ctcaatgcat ggcagtaccg aagcgatggt tcagtatctc
     7561 acgaaggctc tgattgagcg tgagattacg gttcagcctt ttaacctgtc cagaaccgat
     7621 ataggggagt tggcgatggc ggtggtggat gcggctacgg taataatcgg atcaccaact
     7681 gtcattctcg gtccgcatcc agcggcagtc agtgcggctt atttagtaaa ccttttacga
     7741 cctaagaccc ggtacttgac gattatcggc tcttttggct ggggtggaaa aatgcccgag
     7801 cagatcaaag ccctgctaag tggaatgaaa gccgagtttc tgtctccggt aattattaaa
     7861 ggttatccca aattggagga cttttctgcc ctagatagat tagcggatga aattgccgcc
     7921 agacaccgtg ctttaagttc ttaaggagat aattgatgac tattttcann nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnta tacccagaac ctgatttttt ctgatgccaa
     8101 tgatgaaacc aaaataccag caattaatga tgctaaaacg gcattgcttt atgccattga
     8161 aagagaactc gattcggttc tgtattataa agaaatcaag gagttaattc cagccagtga
     8221 gcatcaattg attagcggaa tcattgacga agagcgccgc catgtcgtcc gactttcgga
     8281 attgaagaag aatctgacgt tttaatcaaa gagattctta cccgataatt tggcttaggt
     8341 aatgagcgtg aaaaacgctg tattttgaat ttttcatcgt aatatattca ggaataaaaa
     8401 tgacaataga ggataaaatc gcaattgaaa ttcaagaatg aatatccacg gcgagtttta
     8461 acggcgcgcc agcgtaagcg accggaagat attcgcgggc catttttccg cgatcaaacg
     8521 gctattatcc attcactggc atttcgaaga ctaaaacaca aaacgcaggt ctttttttcc
     8581 ccggaaaatg accatatttg tacccggatt gaacactctt tacacgtggc gacgatttca
     8641 gcgacgattt gtcgagcttt aaaattggac attgatttag caacggcgat cggattgggg
     8701 catgatttag ggcacgcgcc ttttggccat gccggtgaaa aatggctgga gaatttattt
     8761 ggcggcttcg tgcatgaatt acacagtctg cgagtggttg ataaactcga aaattatggg
     8821 caagggctga atctgaccta tgcggtgcgt gatggaatcg tttcgcattg tggcgaagtt
     8881 gacgagcaat ttatcgaaat cagtccaaca cttaacgatc tggacaattt acaatttcgc
     8941 ccagttcagc cttccacctg ggaagcctgt gttgtacgcc tcagcgattc aatcgcctat
     9001 ttgggacgtg atctggaaga tgcagtcgcg gctaatctta ttaacaagga agatttgccg
     9061 gagaaattga gaagaggtat cggacaatcc aacagtgaaa ttatcaataa tctggttttc
     9121 gatgtcattg attggtcaac gaaaaatggc aaaattggtt tttcaccgga aaaatttgcc
     9181 ctgattagcg aatttaaaga atttagtcgg gaaaaaatct acgataatcc aaagctaaaa
     9241 tattggcgga gttactccgg ctttgtaatc gaaaccctca aaaattacct tattgatctg
     9301 tacgaaaaat atcaaaatga ctatgagaga tatgctaata gtagaacacc actggatgta
     9361 aaatttggca aatatctcaa atccttgcat gaacgctatc aggctgaaaa gtgttcttcg
     9421 gaattgatcg tgcgtgatta tatagctggg atgacagatg cttacgcctt acattgcttt
     9481 cagaatatct taacgatggg gacccgcgga ctttgacatc agtgacaacc gataactaat
     9541 gggttagaag cctcttatta tattactgag tttagtactt ttttattttt aacctttgca
     9601 attggtgcag attagcccaa taatttttca gttgagtcat ttataaaaat ttggtataag
     9661 aaattttcac ctcttgcttt gcaattcgat tttcatctaa aaaactcttg aaattcattt
     9721 taatttaact tacaatccga agtggatttt attataaaaa acgtaagctt tctgaaaaca
     9781 aaataaatgg gggatacttt tatgaagaaa aatggttggt tggtgatttt tttgttgtgc
     9841 gttccattaa tggctcaaaa gttcgacaaa atggcgctga cgccaccgat gggttggaat
     9901 agttggaaca ggtttgggtg cgatgtgaat gaacaactca ttcgggaaac agccgatgcg
     9961 atggtgtcca gtggtatgaa agccgctggt tatcagtatg tggtgattga tgattgttgg
    10021 caaggggaac gcgatgctct gggatttatt caacctgatc caggaaggtt tccatctggt
    10081 atgaaagctc tggctgatta tattcattcc aagggattga aatttgggat ttattcggat
    10141 gccggttgga aaacatgtgg tggtcatccc ggtagccgtg ggtatgagta tcaggatgca
    10201 cttacttatg ccaaatgggg agttgattat ttgaaatatg attggtgcaa taccgaaggg
    10261 cttaatgctg aaggggctta tctgactatg cgagatgctt tgttcgcggc tggtcggcca
    10321 atcgttttca gcatttgtga atggggcgat aatcgtccct gggaatgggg caaagatatt
    10381 ggtcatctct ggcgtacgac cggcgacata actgccattt ttgatggaca ggaggggcat
    10441 ggtacctgga attcctgggg tgtgtcacaa attctcgata tgcgcgaaaa tatccgtcag
    10501 tatgccggac cggatcattg gaacgatccg gatatgctgg aagtcggcaa tgggatgaca
    10561 atcgccgaag atcgggcaca tttttccctg tggtgtattc aggcggcacc tttaattgcg
    10621 ggcaacgacc tcagagatat gagttcggag gtgatagata ttttgactaa taaagagttg
    10681 attgccattg atcaggatcc attgggaatc cagggtttta aataccttgt taaagataat
    10741 tgggaagtct gggcaaaacc gctggataaa ggcgatctgg cactctgctt tttaaatcga
    10801 acgttagaac cggtgaaagt tgacctcgat tggaaaaaat atccggtcaa agatgacgaa
    10861 tttcattact attatctttt taatgaaaat gaatacaaaa ttcgtgacct ttggttgaaa
    10921 aaagagattg ggacaactgc caaaccgttt tcgactcaaa taggtgctca cgatgttatt
    10981 gtattaaggc taagtccggt caagtaaaac taccgaccgg tcgggaaatt tatatctttg
    11041 ggcattccat tataactgac aactgcttcc ggtttaccgc aaagtagaaa actgtgattg
    11101 cggtgatcac gttcgtcgag tccgattcgt cggaagacat ctgcccagga gctaaatttt
    11161 ccgtattcat tgaccaggtc gctttcgacc ggctgagttt cccattccgg gtgatcggca
    11221 acgaattggg catagacatg ttcggcatga tcctcgaatt cagcattgaa gagataagcg
    11281 cgccggatat tgcagaaagc caaagttttg gcaaaaaggc gatatactgc aaccattaca
    11341 tacggaacga gcggaaaaag gtaccaagca tctcgaatac catcggtttt cattttttcg
    11401 tgaatgacca gtaaatgcca gtattcatta tcctgggctg atcggctcca ggtgacaatc
    11461 cgttgacttc gagtgacttt ttcataattg cggtaaacgc gggtcagacg agaataatga
    11521 cgtacttccc aggcgcgata cgggatactg gctaagattt cgattaattt agctttagat
    11581 aaggtaatct ggtacccagt aacaatatcc atcagtacaa agaaagcacg tgccatcaac
    11641 gagtatttat aatgaagccg ttcaagagaa ctgatctgtt cttttttaag atcaataatc
    11701 tcggtattat tattcatgct ttatccagcg attttaatca acaatcagga atataattaa
    11761 aatcggtagt tcaaacaata aaccagtgga atttgcattg caattccata tataactggc
    11821 tatcctctat ttaagtagtg gatagccagt tattattaat ttggcttgga tttagccgac
    11881 gtacagcaga ctaatagcgt ccgccgccgc gccgattgtc ccgcggtttg ccaccgaagt
    11941 gaccgctacc agaatggccg ccgaagcgat cgccaccgcg accgccctct gtacgaggac
    12001 gagcttcatt gacggtaatg gttttgccct tcaagtcttt gccgtttaaa ttgctgatag
    12061 cggcttgagc ttcagccatt aaaggcattt cgacaaagcc gaagcctctt gattcgccgg
    12121 tgaatttatc tttaataaca gatgcactgg cgacctgtcc gaaagcctcg aaagcaacgc
    12181 gtaagtcacc ttcagtgaca ttcggtgcta aatttcctac gtagatgttc attatgacct
    12241 ccttttgatc ttgaacgctt taacgtactt tcttttacat tgaccaaatg cttatctcta
    12301 agcgggttcc ataacccaac tccagaaagt agtttccgcc agtacccgca aaatgggtat
    12361 tttcgtgatc aaaaaaggtc taccactaag tgaaaaagtt tttctggttg gtttgtgatt
    12421 gatggttact cgtttttcga aaagcgttgt attaaaatac tttaaaccct gcagttaaac
    12481 aagctttatt ttttagagtg ttaattgtag tagtttttgt gaataaaatt ttctatgtct
    12541 tactggatta ataaatagaa catattactt aataattaat aacttaccag catgcgcgcg
    12601 ttgcccagcc tgaagacaat aaatgtaaac gccactgctc agattgctcc cactccaagg
    12661 tacaatatga tgaccggatg gaaatcgtcc taactgttgt tcgttaacta aatggccatt
    12721 taaatcgaat accatcagag taacattttc aatctctggt aaggtgaatg ccaaattcgt
    12781 ggtgttatta aaaggattcg gataacaggg atggattttg aagtcgccgg tcaagtttgg
    12841 tttttctatt accaaggtgc tgggtttata gaaccagatt ctccgcgcca gggttgaatc
    12901 cagcacgaat ggattgggtt tgttatcctg ataagtggcg attttccacg tacgcgcata
    12961 ttcgttggca tcaacggtgt cgtaataatt ccaggctaat aaagtttctt tttgaaaatt
    13021 ccaaaaagtc atatcggggt ctttagtaaa gtatacctcc gaatacatgg catagaaata
    13081 aaacatcgaa cgagcacaat tgcctttatg atcttcacgc ggttcaaaac aggctggggg
    13141 atcgttttct ttttccgagt atttatcaat attggaggtt ggaattatgg tttgtgactt
    13201 atttaaatac caccatttat cagttttagc atccgggatt tcggcaaaag gatcattgcc
    13261 gcgtgaagaa ttcacgttgg taaaggtcgg atacaaatgg tgtaaattgc ttctctgagg
    13321 ttcagaatcg gcgcccattg attgtggcca agtatgttcg caatcaattg atttgttata
    13381 agcgtcggtg cttgggtctt tggtacggtc aagtttgatc gtgtaaccgg tatagacaca
    13441 ggagagtgaa ccaccatttt ttaaatcaat cacggcatac attgtatcgc gggatttatc
    13501 atagcccagt gttttaatcg gtttaaaatt agcaattact gaatcccata gtgctgtgcc
    13561 attcaattct ggccatagaa tcgtttgaac ctgtgaatgt cccaaagaga aaaaggaaat
    13621 gattgacaaa aggagtatta aggaaaaaga tagtcggggc ttttttatcg ttttttctaa
    13681 caaattaatc tctttcggct ttctttttgc gacgttcttc aataatactt tcttcaatac
    13741 tttttggtaa gggagtataa tgtaagaatt ccatcgaaaa agccgcacgg ccgctggaaa
    13801 tagtgcgtaa agccgaggca taaccgaaca tttccattaa tgggactgtg ccattgagtt
    13861 tttgtgagcc tttgcggtga cggcgcatat tttcaatttt accgcgccgg cggttgatat
    13921 caccgatagc atcgcccata tattcatccg gcgtattgat ttcgattttc ataataggtt
    13981 ccaataattg cggtgccgtt ttattaatga tttccttcat ccccaggatc gtgcacattc
    14041 ggaaagccat ttcgctggaa tccaccgggt gataagcgcc gtcgaaaaga acgaatttaa
    14101 tatctaccat cgggtaaccg gctaataaac ctttttcgac gatctcgcga aaaccctttt
    14161 cgaccgccgg gatgaattcg cgcggaatat taccaccctt gacacgatcg acaaactcaa
    14221 taccctttcc tggatttggc tcgagccgga aagcaatctg agcaaattga cctttgccgc
    14281 cagtttgttt tttatagcga taagtatgtt cagcttcacg tgtaatagtt tcgcgaaaag
    14341 ctaccgccgg tttaccgaca ttgacgtcga gtttgtgttc gttctttaac ctatctacga
    14401 tgatttcgag atgtaattcg cccattccgg agatgatagt ttcttcagtt tcgtcggcaa
    14461 atcgcacttt aaatgagggg tcttcaagag ccaagcgact caaggctatt ccaagacgat
    14521 ctcgttcttt gattgcgagt ggttcgattt tcatatccag tacggtttcg ggataacgaa
    14581 tattttcgag taaaatagta tcattctcgt tacaaagcgt atcacctgta gttgtcagtt
    14641 tcataccgat taaagcgcca atatcaccgg ctttcaagga tgtaatttct gtacgctcat
    14701 cggcgcgaat acgcagaacc cgtccgacac gttcccgctt ttgcttggta gaattgaaga
    14761 tataagaacc ggaatgtaat tctccggagt aaacccgcac gaaagtctgt tgacccacgt
    14821 aagcatcatt agtgattttg aatgccaacg ccgtcagcgg ttctgaaata tggggtttac
    14881 gtgaaatgat aacttcagga ttattagcat cacgtccggt aattattccc cgatcaacag
    14941 gggacggcag gtaatcaact atggcgtcaa gtagtaagcg aataccttta tttttgaaag
    15001 ccgagccaca gaaaaccggt gttacacata aatttaagac ggcataacga gcggcattac
    15061 gaatgtcgga ctcactgatt gccataccat tgataaactt ctccatcagg ttttcgtcga
    15121 aatcagccaa tttttcaatc atctgctcgc gtaatttttg ggctttcggc aataaattag
    15181 atgggatttc gctttcgaac atatcaagtt cgcgatatga ataggctttc attgtaatta
    15241 agtcaatgat tccattaaaa tcggcttcat tgccaattgg caattggaaa gcgaccgcat
    15301 tagcacccaa catttcgttc atcatattaa tagtctgaaa ataatcggct ccggcgcgat
    15361 ccattttatt gataaacgcg atgcgcggaa ctttgtagtt gtcagcctgg tgccagacgg
    15421 tttcactctg cggttcaacg ccgcctactg cacaaaaaac cataactatc ccatccagaa
    15481 cacgcaaaga gcgttcgact tctactgtga aatcgatatg accgggcgta tcgatgaggt
    15541 tgatttgatg atctttccat tcggtggtga tcgccgccga agtgatcgtg atgccgcgtt
    15601 cttgttcctg cttcataaaa tccataacag cttgaccatt atggacttcg ccgattttgt
    15661 gagtcatacc ggtaaaaaag aggatacgct ctgaagtcgt agttttaccg gcatctatgt
    15721 gggctacaat tccaatattt ctaatttttt gtatttcgat attatccatt tattgattcc
    15781 taataggact aaaaatagct aaaaaattta atcaaataat cagcgattag aaagaattat
    15841 tgttcagaaa actgtctgat ctagggacat ggtaaccact tttggtgatg atgttcaggt
    15901 agtgatcggt gatgataggt gggaagatgc cgatggtttc aggattaatt tttggcgggt
    15961 tatattcatt cctggttgtc tgccaggtag gaggaaaaga gtcagattta gttgctccgg
    16021 tctcaataga actggatgcg acgatggtta gtggtcttga agcggaagta gatcatttta
    16081 agattaagag tagcataaag tatagccaag tagttcaaat ggtagagaag cgtcgtcgtt
    16141 taatggataa agtagcttat ggtagtgtta gtgatagtga gacatttggc gagaattttt
    16201 atgctttttg tcgtgataat ggactggatt tacgccggcg ggtattgtta ttgagttatg
    16261 gtgcggatgg attaacagtt tggcggagaa cgtatttatg gaggcagaga gaaggctgga
    16321 tttagcgcgc ttggggttcg gtgttggatg gcgacgacgt ttctgaatta agtcaaacca
    16381 tatggttttc gagtggcgaa cagataatta gatatatcca ggaaatttgc cagtggaaaa
    16441 gtctcaatgg tgaatccgtg gatgacttga tgacttacta actcaataac cggaaataaa
    16501 tccattatct gtccggcgaa cgtcatggtt ctggcggcgt ggaaaagagc atcggcatct
    16561 tgattggtca ccgttttaaa aaacagggta tgtctcggag ccatgaaggt accaataatc
    16621 ttctggcgtt acgagcagaa aagttaaatc agatatagca ataagaatca caagctttaa
    16681 attataccag gtgaacaaca tatcttttga catgcttccg ttctttttta acgaacttgc
    16741 ttaacctatt ttgattcctg atttacccct ttttagtaat ttcaaaaaac agttgctttt
    16801 aattattgga atgttttata ttaaacttgt aagatgtatt acaaatgaac aaattaatag
    16861 tgtaataaat gcaaaattta tttccaaaaa ttggcactga tttaactctc agtcaagaga
    16921 ttgttaaaaa actcgaagat attattcgtc agaaaaaagt actgcctggt gaaaaattac
    16981 ctagtgaaat ggaaatgtgt tccatgtttg gggtaagtag aacttcatta cgggaagcct
    17041 tacagatgct tagcgccaga gggctgatta cgatccgaaa gggtagtggg atttatgtaa
    17101 ataattattc cccagctcac gcaatcaaac caatgagcct ttttttagag ttgaatttag
    17161 atcgtgagta tatcacgcat gtgatggaag tgcggaaaat gtttgagcca aatatagcta
    17221 gtctagctgc gctaaaccga actgaaacag atattcagaa actggaacag aatcttgaag
    17281 aactaaaaaa ttcgcctatt gataattttt ataaacaggg ggagattgac agagatttcc
    17341 atctaattat agctgaggcc tgtaaaaatt cgatcgtaca aatcattgtt actccaattt
    17401 ttgaattaat gcctaaaatt cgttcaattg tttatgccta tgtacagacc gccaaggacg
    17461 aagcgattga ttttcatttc aagatatttg aaaaaattaa agaaaaagat gctgacggcg
    17521 cttatcaaat gatgataaaa caccttgaaa tagccgaaca gcattcaagg aaagtattgg
    17581 atgaaatata attaaatttt cggcaatgta tgatatattg ataaaattaa catttaaata
    17641 taaataagga gaaaagtcat gaatattaat cttactagaa aaacagcaat cataactgga
    17701 agtgtccagg gaattggctt tgacatagcc aagatattta ccgaatgtgg cgcatcggtt
    17761 gttattaata ataatcttga cgctgattca ttggaaataa aagcagaaga attgcgtaaa
    17821 agtggagcag atgtaaaagc agtcgttgcg gacgttacta taaaggaaga agcgcaaaaa
    17881 cttatagatg cagctttatc gttcggagat acgattgata ttttggttaa taatgctcgt
    17941 gggttgatca agcgtgtccc aattgctgaa tttgatgaga atcatttttg gacagtcgtt
    18001 aatgtcaatt tgaaaagtgc ctttattatg tctcatttag ttatgcctta tatgaaggaa
    18061 caaaggtcgg ggaaaatagt aaatctttca tctcaagctg cgcatgatgg tggtggtagt
    18121 ggtgccgcag tatacgcagc atcgaagggt ggtatctgga catttacaaa gtcgcttgct
    18181 aaagaagtag cgtcatttgg tatttgtgtt aattgtattg cacccggttt tattgaaaat
    18241 acggcctttc acaatacttt tagttcaaag gaaactcatg agaaagttct agctactgtc
    18301 ccgcttggga gacttggaac tgtgcgtgat atttcccgcg tagctctttt tctggcatct
    18361 gaactttcgg attatatgac tgggcaatca cttcaggtca atggtggcct ctatatgcca
    18421 taattaaaaa aatggaatgg taaaatgaaa aaccgattat ttttattctt atttttttcg
    18481 tcaattataa ttttttcatg cgggcaaaac caggcttcgg aacatccttc gcttgtaatc
    18541 agtgc
//
LOCUS       DAOOMU010000042        17624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_3340_length_17624_cov_16.155217, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000042 DAOOMU010000000
VERSION     DAOOMU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 17624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17624
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3340_length_17624_cov_16.155217"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..653)
                     /locus_tag="PLI55_05585"
     CDS             complement(21..653)
                     /locus_tag="PLI55_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPB00340.1"
                     /translation="MINLLIVDDHPLIRQGLRQTFLDTNDIRVSGEASDSASALAEIE
                     NHNYDAVILDLSLPGRSGLDLLKQIKNLSPSLPVLILSVFPESQYAIRVLKAGADGYI
                     SKESHPDELIHAVRKIVTGGKYISPTLAEKLANSLTEATQNPTHEILSDREFQTMLAI
                     ASGKTVKEIAAELNLSVKTISTYRTRILRKMHLKNNAEIICYAVRLGLVQ"
     gene            complement(653..1837)
                     /locus_tag="PLI55_05590"
     CDS             complement(653..1837)
                     /locus_tag="PLI55_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HPB00341.1"
                     /translation="MNLPDSFTSDESKTREQLLWELQQLRNRINQLEAADLERRRIEK
                     SLNKYEKNFRELVNLLPQTIYEMDKNGKFRFTNQVGFETFGYNQQDIDTGVNFSQLFI
                     PEEREKLNRNIQKIYNGSDLEGHEYTALRKDGTTFQALIYSSPIFYDNKPVGLRGVVI
                     DITDRKKVEQDLKDSRDQFRNLSAHLQSVREEERSYIAREIHDELGQALTALKMDLIW
                     INKRLTTAQSEIAEKISTMFDLIDKTIQTIRRISTDLRPGLLDDLGLTAALEWYCEDF
                     QNRTGIHCNLHLRPAEITIDQERSIAIFRILQETLTNVARHAHASEVKVNLGIKDHAL
                     EMKIVDNGIGITDEQVNDPQNLGLVGLRERVYPWNGHITIKGRPQKGTTVLVFIPLDG
                     NI"
     gene            complement(1840..2325)
                     /locus_tag="PLI55_05595"
     CDS             complement(1840..2325)
                     /locus_tag="PLI55_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00342.1"
                     /translation="MNLKKKISIGGMIELRDLMMVSIFSFPDQPGSAGKVLTYFGQRG
                     ISVDFITESRNLEGSADLIFCINQKYKSDITQTFQELSAIIPIKDTKITEPVAIVSFY
                     GPHFREKPAIAGTICQTLGEAGINILGISTSISSVSCVINEHQVDAARQAITANFNLP
                     E"
     gene            2529..5102
                     /locus_tag="PLI55_05600"
     CDS             2529..5102
                     /locus_tag="PLI55_05600"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HPB00343.1"
                     /translation="MSIVNNSLYCIPIYNPYYSLPFPSQAEINELIDNLLNSLKEGTQ
                     KGVYHFDQMIYPLCEYLKSHPEKTDLVRKLVEEKKIYFGPFFIQLHSGHISGELLIRN
                     LLIGHQEGDKIGDILKYFHLPAMRGRNSQTPQILAGFNIDAVLIDYDIKLMDKNTTEF
                     IWEGLDGTQLLVSRVVFLNPNRLNLAKSKIQETFREYFSHNPNRVLVYLFNDKKSWET
                     MPTYIEKLASVFSSDLKIESLADCIWEIKEQIDSREISEYKGEMCLEKTEDCTETQFD
                     YYLRHSVLIGLSNHRLENLIQYFVEPWSLIMKYLQLKIRPKATNHIWEEFLKNQTFTY
                     LVDTNPNKFIQSLSTTQTGLMNRIEEQVKDLITAVVNNIHIDDYNPNNIYFTVFNSLP
                     YNRSEIVEFVMEMPSLINRDTIVAKEVEGREISFRVLHKETETLFKYDKADKAKYRCL
                     VELRDLPGMGWKTFEIDLNGRPKSYPAVPISAERNSLENDFLRVEINQNGTLEIFAKE
                     TGEFFSEVAYFQDEIDLGRFSVEDKTTTHIPLTTKKLHPQIKLLYNSPKAAAYKIEYF
                     WEIPEMFNWKNSRRSPRHETIKISEIVTLSSLSRMIDLKIEINDHACDHMIKICFPIE
                     FVPENTYTDGFFSVETRPLNGLQNLHCQAFSMGNFVGVSNESGGFAIFSDGINEYQLI
                     KGKHNVLTLTLVRDFPIVRSKKTGGPILSRNEKTEVHLSIYPRLSDWTSSDILTEAII
                     FNNPIIIRQMDQTNGYLPAKMQFLKVTPDTLFYSALKPTEDEKDVVLRLYNPTRNIIV
                     GEIATYIPIKAARYLTVEEHIIAPLEISDPYRLKVKVFPQKIMTLKIIFDRLPINSSS
                     S"
     gene            5186..6238
                     /locus_tag="PLI55_05605"
     CDS             5186..6238
                     /locus_tag="PLI55_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPB00344.1"
                     /translation="MFANQDKSSIQKLEEFVSELSRNHITAQKEAPLAKFTTFGIGGN
                     ARLLVIVENTEKLEAVIKLATAFQVPFRIIGCGSNLLVSDDGYDGLIVVNRSKSWQII
                     DDCPANLKFSRIIPRYDPLTASRTEEIQAENLSQKIVRVDSGFYNNTLIQEMFEQGLT
                     GLEWFSGIPSSVGGAVYMNMHGAHTYFGDLVYRAKLIDGISPKIVDNAYFQFDYDFSV
                     LQKTREVILWVELILTMGNVEQARIVAQEWKSRKSFQPRRSAGCIFKNLSPEAQKQFN
                     LPTSSVGYLIDKVLNLKGLRIGGAVISPKHAAFIENDGTARADDVYKLIKIIKAKAQE
                     TLGLELETEVELLGKF"
     gene            6256..7515
                     /gene="murA"
                     /locus_tag="PLI55_05610"
     CDS             6256..7515
                     /gene="murA"
                     /locus_tag="PLI55_05610"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPB00345.1"
                     /translation="MSRFIIEGNRNLSGEIKVAGNKNAALPIIAATVLTDEPCILENL
                     PDISDVRSMLAILSDLGKSVEMLSAHKCQITGSVNKSRLNDKLASTLRASILYLSVLL
                     ARTGEVEMVPPGGCVIGRRKVDQHFDALQALGVKVNIIEHGYRLILKRPKPNYVFLRE
                     ASVTATENAMMLAAMTEGKTTLDNAAGEPHVQDLAKVLVKMGAKIQGAGTNRLTITGT
                     KKLKGFRHRIMPDHIEAGTFAIAAACTGGEVVIHDANRENLTIINLILDQMRVKLSFL
                     DEKTLVIKPSKNIRAIPKVQVGVWPGFPTDLMSPMVVLATQARGTTLCHDWMYESRMF
                     FVDKLVTMGAQIVQCDPHRVLVSGPTRLKGQEIVSPDIRAGIALVIAALIARGKSKIE
                     KAELIDRGYEDIVPRFRQLGAEINREQ"
     gene            7621..10182
                     /gene="glgP"
                     /locus_tag="PLI55_05615"
     CDS             7621..10182
                     /gene="glgP"
                     /locus_tag="PLI55_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPB00346.1"
                     /translation="MKPIQSFTVKPILPKKLEELRELAYNLWWTWNADARDLFRRLDM
                     DLWEETRHNPVALLNRVEQDVLETRAQDDSYLYQLRRVYDKFKQSLNSPTWFEKSGKY
                     NSHLNIAYFSMEYGITECLADYSGGLGVLAGDHLKSASELGIPLQAVGLSYQYGYFQQ
                     VLNVDDYQEEEYPVNDFYNSPLTLVKGSDGKQLLIKVPFPGRLVYARIWKAQVGRVPL
                     YLLDTNIAENSEADKKITAELYGGNTETRIQQEIILGMGGFQALQILDATPFICHMNE
                     GHSAFSGLERIRNVVKNNGLTFYEALEIIKAASVFTTHTPVQAGIDLFHPDLIKRYFD
                     EYCREVGIEINELLALGRNDPDNNSEHFSMAILAINLSSKINAVSKLHQQVAQEMWES
                     LWPDILTTEIPIDYVTNGIHQASWISQEMSDLFDRYLGPDWRREPASKTIWNKVDKIP
                     DGELWRTHERRRERLVAYARSELYRQYVTLGKPQNIIARVKSILSTDALTIGFARRFA
                     AYKRAYLLFTDPERLAKILTNKNFPVQIIIAGKAHPQDEEGKQVIRRILKLSQEEPFY
                     QCVVFLENYDMNMARYLVQGCDLWLNTPRRGLEACGTSGMKAVANGGLHFSTIDGWWD
                     EVYTPGIGWAIGTRDIYEDIDYWDRQEANILYNVLENEIIPTFFERDNDDLPRDWIAM
                     IKASMKAICPQYNSNRMVCEYLDQIYYPAAVRANELSKDNYAVAKELAHWKEKMRKNW
                     DLVRFLKVTSGSIEELSVNSQLEIRAEIYLDGLQVDDVDVQVYYGRVDPNGDIIDGKF
                     SSMKFETKVDSKKYVYVDKINNWESGLNGYTIRIIPKHPALAYPFEDGLIHWFDE"
     gene            10241..11008
                     /locus_tag="PLI55_05620"
     CDS             10241..11008
                     /locus_tag="PLI55_05620"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPB00347.1"
                     /translation="MNLITNEVARKRISQGEQVVVLTGAGISAPSGIPTFRGENGLWT
                     KLSPQELASFESFYLNTAMVSEWYKHRREIIEVCQPNAGHYALVELAKMVSKFTLVTQ
                     NIDGLHQRAGSRDVLELHGTIMENYCIRCGQQYSATEFDEIYNSSFDHIPRCYCGGLI
                     RPNVVWFGESLPKQAYEEAYRASQQADLFLSVGTSAQVRPAADLPLIAKENGAFLVEI
                     NIVETVLTPFTDLFLKGAADEILPQFNWEYQYLLMQV"
     gene            11036..12895
                     /locus_tag="PLI55_05625"
     CDS             11036..12895
                     /locus_tag="PLI55_05625"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00348.1"
                     /translation="MKNNITVKSIETLFLIGLILVLSNCAYYNTYYNAEKYFREAEKE
                     FKTLPDEKISPALKKKFDTAIEKANKVITQYPESRWVDDSYYIIAMSHYYKGDYRIAR
                     QKFEDFAVKFPQSKLYPEAMVWYGRNLWKLDERELAFFQWKKIMNLTDDYYLLAELYL
                     SIGELYFKDSAYDSALFYYQKATEVGGSFDIAAEAQFRIAEIDLALNRPQEAIKNLKK
                     IDQFSPSLILRDKMQVLLVRIYRESGQYDKAIDLINEKLNDPANEKIWGDLELQLGLI
                     YLAQQDYDAAISRLTKVIEDYKGKPVAAEASYHLAELYMTQIHDYTKAAVQYDNVVKI
                     EVNTLRAFECRNKSSEIKRFNTIYNRLAALSEQVAAINLNPPTESDTLGESLPVDQEQ
                     SELKKALEKKNTTQKKTIDTLAVFKEYYTTLYEIAEIFYFSFNQPDSAIYYFEKVAYE
                     IPFNSLRDKALYSLYRIASDRQNIEKANEYANQIKQWFPESIYLAEIEKRPLNTSARE
                     VEADSLLVKAENLDQTDLHSAITIYEKLAKEYTDCQAAEKAVMYLAYFYHHRLFDLNN
                     ALTWYKYYLDTYPNGQYYQIMANAYDQLKEIEDALAQGSEAEENEPQKPEKVE"
     gene            12892..13659
                     /locus_tag="PLI55_05630"
     CDS             12892..13659
                     /locus_tag="PLI55_05630"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPB00349.1"
                     /translation="MRVLKDTTVLSNRMVATDTYLMEFAFPEISQSAQPGQFVNFYFP
                     ERPQLFPRPFSIAGVNRENLTILYKKVGCLTSIMSQWQPSQKIRVLGPLGNTFNIKYN
                     NHVLIAGGVGLAPLLFLNQKLANLNYKIWNFVGVRSKDELPPLSLLSGEKYIATEDGS
                     VEYCGTVVDCLRNNLGAIPHPFSVYACGPEAMLSALWHLSRENHFDLQLSLEKLMACG
                     LGICQGCAIPHSHPSEQPLRLVCQDGPVFNANELDLG"
     gene            13656..14570
                     /locus_tag="PLI55_05635"
     CDS             13656..14570
                     /locus_tag="PLI55_05635"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HPB00350.1"
                     /translation="MTDLHIKLREDFILRNPLLTASGTYGYGDEIADLINPELYGGIV
                     TKSISLEPRVGNPPPRIAEVTSGMLNSIGLANIGVDKFLEEKEEFLAAYNGKAIVNIA
                     GRKDEEYVQVVKKLSDRQWIAAFEINVSCPNVSEGGIEFGRDPLILGQLVKKLRHATD
                     KFLIIKLSPNVTDIRVLALAAQENGADAISMINTVYGAAIDIHTRRPKLNTVVGGLSG
                     PAVKPIALAHIIKTYKEVDIPIIGIGGISCGADVIEFLLAGAHAVQIGTANFFNPKVC
                     GEMLDFIEQYCTENKISCLRDIIGRVEV"
     gene            14534..15028
                     /locus_tag="PLI55_05640"
     CDS             14534..15028
                     /locus_tag="PLI55_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HPB00351.1"
                     /translation="MSARYNRTSRSLSLIVILLLFMMLGFMGCASAPRYLYTSPKTGA
                     TNDDKNTLLLLKNSNRTAVLTASYYGADFHGKLTSSGEVFDMNGMTAAHKSLPFGTVL
                     QVTYPPTGQSVTVVVNDRGPFVPGRDLDLSYAAARKIGLVNDGTGKVKVKVLKWGNND
                     SPKQ"
     gene            15044..15847
                     /locus_tag="PLI55_05645"
     CDS             15044..15847
                     /locus_tag="PLI55_05645"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPB00352.1"
                     /translation="MNNFASRMLINIIGIPAILLLIHLGGIPFVLFITVMVFIAQVEL
                     YLLMRKKGIAPLLLPGVLAGVIWQLASFYLPGALLLYLLIGLVVLLLLASLVRQIKNV
                     TVDLASTLFGFVYLPVLTSTIILMRNIPGRGRSIVLLLFVTIWVCDSLAFIIGKWLGR
                     RKIAPKISPNKTYAGCIGGLFGAVLTVLIFYFLGWAPDFMSIMQLIIFGILAGVFGQA
                     GDFVESAFKRDAEVKDSSNLLLGHGGVLDRFDSFFIAAPVIFLYIITIL"
     gene            15895..17403
                     /locus_tag="PLI55_05650"
     CDS             15895..17403
                     /locus_tag="PLI55_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPB00353.1"
                     /translation="MSINNGNNDLVEYLHSLSGEIDPKQGSVAIILAAGHGKRIKSER
                     SKMLHEIWGVPTVVRVANAASKGLASNNQIIVVGIKACEVAESAGKRANRIFVYQTEQ
                     KGTGHAVKEALSAISQNGHIKDIYIFPGDMGLITPAVVSELKRRFEQSGCGLALLTGN
                     YKGEPEANSYGRIIRVPARTATGEPSGEDEGRVIEIKEHKDILAIPESGFYTTVYNGK
                     KYQFKRSELLGITEYNTGVYAMKFDLAQRFIHQLTPDNVQGELYLTDIIGLFNANKIE
                     VEAYPVDDESAVIGFNVKSVLQEMENIARRKVWKLLRDIIYIEDENDFFIADEVVIRI
                     LEMDKINPPLDIEIGKGVHISSGVHLERGVKIRAGSLLNGNIHLGVGTVVQENVSLST
                     YPNQQMTIGKNCVIMRGDLIKGNITIGDNTSIESSVIITGSSEYPTIIGKNVVIKGTT
                     YIFGSIIEDDLFIEHSVLKCKRVERTVRKDGTIQPVRWVMPQPQGLDIVRDL"
BASE COUNT         5143 a         3368 c         3911 g         5202 t
ORIGIN      
        1 attgtcttat aaaattaact ctactgcact agtcccagcc ggacggcata gcagataatt
       61 tcggcgttat ttttcaggtg cattttgcgt agaatgcgag tgcgataagt gctgatagtt
      121 ttgacgctga ggttcagttc agcggcgatt tccttgacgg ttttgccgct ggcgatcgct
      181 aacattgttt gaaattcgcg gtctgaaaga atttcgtggg tgggattttg agtggcttcg
      241 gtaaggctgt tagccaattt ttccgcaagt gtgggagaaa tgtacttacc accggttacg
      301 attttacgaa cggcgtggat tagttcatct ggatgacttt ctttgctaat ataaccatcg
      361 gcgccggctt tcaatacccg aatagcatat tgactttcag ggaaaacgct gagaattaat
      421 accggtaaag aaggtgaaag atttttgatt tgtttgagaa gatctaagcc actgcggccg
      481 ggcaatgaaa gatcgagaat taccgcatcg tagttatggt tttcaatttc agcgagggcg
      541 gaagcactat cgctcgcctc gccgctaact cgaatatcgt tggtatcaag gaaagtttga
      601 cgtaatccct ggcggatcaa aggatgatcg tcaactatta gcaaatttat cattagatat
      661 ttccatccaa tgggataaag accagaacgg ttgtcccttt ttgcgggcga cctttaattg
      721 tgatgtggcc attccaggga taaacgcgtt cgcgcaatcc aaccaagccc agattttggg
      781 gatcattgac ctgctcatca gtaattccga tgccgttatc aacaattttc atttctaagg
      841 cgtgatcttt aataccgagg ttgactttga cctcgctggc atgggcatga cgagcgacat
      901 tcgtcagagt ttcttgtaaa atacggaaaa tcgcaatact acgttcctga tcaatcgtga
      961 tttccgccgg tcgcagatgt aggttacagt gaatacctgt ccgattttga aaatcctcac
     1021 aataccattc gagagcggcg gtcaagccta aatcatcgag caaaccaggt cgcaagtcgg
     1081 ttgaaatccg ccgaatagtt tgaattgttt tatcaattag atcgaacatt gtgctgattt
     1141 tttcagcaat ttcggattga gcggtagtta gtcttttatt gatccagatc aagtccattt
     1201 tcaaagccgt tagggcctga ccgagttcat cgtgaatttc gcgagcgata taggaacgtt
     1261 cttcttcgcg gaccgattgc aaatgcgccg aaaggttacg aaactggtcg cgcgaatcct
     1321 tcaaatcttg ttcgactttt ttccggtcgg ttatatcaat gaccacaccg cgcaagccga
     1381 ccggtttgtt atcgtaaaaa ataggactgg aatagattag ggcttggaaa gttgtcccgt
     1441 ctttccgcaa ggcagtatat tcatgtccct cgagatcgct accgttataa attttttgaa
     1501 tattacggtt aagtttttcc cgttcttccg gaataaaaag ctgggagaaa ttaactccgg
     1561 tgtcaatatc ctgttgatta tatccgaaag tttcaaatcc gacttgattg gtaaagcgaa
     1621 atttgccatt tttgtccatt tcataaatag tttggggtag gagattgact aactcgcgaa
     1681 aattcttttc gtatttattc agggattttt ctattcggcg gcgttcgaga tcagcggctt
     1741 caagctggtt tatccgatta cgtagttgct gtaattccca gagcaactgt tcgcgggttt
     1801 tactttcgtc actagtaaat gaatcaggaa gattcatttc tactccggta agttaaaatt
     1861 cgcagttata gcttgccgag cggcgtcaac ctgatgctcg ttaattacac agcttactga
     1921 agaaatactg gttgaaattc ctaatatatt gattccagct tcgccaagtg tttgacaaat
     1981 tgtcccggcg atagctggtt tttcacggaa atgagggcca taaaaagaga caattgctac
     2041 tggttcagtt attttagtat ccttaattgg gataatggca gaaagttcct gaaacgtctg
     2101 ggtaatgtct gatttgtatt tctgattgat acagaaaata aggtccgccg agccttccaa
     2161 attgcggctt tcggttataa aatcaacgct aatccctctt tgcccgaagt aagtcaaaac
     2221 ttttccggct gaacccggtt gatcggggaa tgagaaaatc gagaccatca tcaagtctct
     2281 taattcgatc attccgccga tactgatttt cttttttaaa ttcatattgg ctctgtttgc
     2341 aaattgttga aaaataggtt aattatttga aaaaatccag tccgattttt aacagccgat
     2401 aaattaatac caatcttcat ctattaattt taaaaaataa ttgaaaattt cactaaatat
     2461 gtgatatatt ttagaaaaca agttggattg ggcaaattcg gcttgtatga aattaaaaag
     2521 tagatgtaat gtcgatcgtt aataacagtt tgtattgcat accaatttat aatccctatt
     2581 attcattacc ttttccttct caagcggaaa taaatgagtt gattgataat cttttaaata
     2641 gcctgaaaga aggaacgcag aaaggggtgt atcatttcga ccaaatgatt taccctttgt
     2701 gcgaatattt aaaaagtcac ccggaaaaaa cggatttagt tcgcaaatta gtcgaagaga
     2761 aaaaaattta tttcggaccg tttttcattc aacttcattc cggacatatt tccggtgaat
     2821 tgttgattcg taatcttctc attggccatc aggaagggga caaaatcggt gacattttaa
     2881 agtactttca tttaccagcg atgcgcggac gcaattcaca gacgccccag attttggctg
     2941 gtttcaatat tgatgctgtt ttaattgact acgatataaa gttaatggat aaaaatacca
     3001 ccgagtttat ctgggaaggc ttggatggta cccaactatt ggtcagtcgc gtggttttct
     3061 taaatcctaa tcgtttaaat ttagcaaaaa gtaaaattca ggagacgttc cgcgaatatt
     3121 tcagccataa tcccaatcga gtcttggttt atctgttcaa tgataaaaaa agttgggaaa
     3181 ccatgccgac ttatattgaa aaattggctt cggttttttc ttctgatttg aaaattgagt
     3241 ctctggccga ttgtatttgg gaaattaaag agcagattga ttcgcgtgaa ataagtgaat
     3301 acaaggggga aatgtgttta gaaaaaactg aagattgtac cgagactcag ttcgattatt
     3361 atctaagaca tagcgtgttg attggtttaa gtaatcatcg tcttgagaac ctaattcaat
     3421 attttgttga accgtggtca ttaattatga aatatttaca gctgaaaatt aggccgaagg
     3481 cgaccaacca tatttgggag gaattcctaa aaaaccagac atttacttat ctcgtagata
     3541 ctaatcccaa taaatttatc cagtcactta gtaccactca aactggactg atgaatcgga
     3601 tcgaagagca ggtaaaggat ttaattacgg cagttgttaa taatattcat attgatgatt
     3661 ataatcctaa taatatttac tttacggtat ttaattcttt gccatataac cgatctgaaa
     3721 ttgttgaatt tgtcatggag atgccatctt tgattaatcg ggatacgatc gttgccaaag
     3781 aggtggaggg acgtgaaatt tcgtttcgag ttctccataa ggaaactgaa acactattta
     3841 agtacgacaa ggcagataaa gcgaaatatc gttgtttagt agaattgcgc gacctgcccg
     3901 gaatgggctg gaaaactttt gaaattgatc tgaacggaag gccgaaatcc tatccagctg
     3961 tgccgatcag cgccgagcgc aattcgttgg agaatgattt cttacgagtt gaaattaatc
     4021 agaatggaac gctggaaata tttgccaaag aaaccggtga atttttctcg gaagttgcct
     4081 attttcaaga tgaaattgat ctgggacgat tcagtgttga ggataaaacc acgactcata
     4141 ttccccttac tacaaaaaag ctccatcctc aaatcaaatt gctttataat tctcctaaag
     4201 ccgccgccta taaaatcgaa tatttttggg aaattccaga aatgttcaat tggaaaaatt
     4261 cgcgtcgcag tcccaggcat gagacaatca aaattagtga aatagtaacc ttaagtagcc
     4321 tctcccgaat gattgattta aaaatagaaa ttaatgatca tgcctgtgac catatgataa
     4381 aaatttgttt tcccattgaa tttgtaccgg agaatactta tactgatggc tttttttcgg
     4441 ttgagacgcg cccgcttaac ggcctgcaaa atcttcattg ccaagcattt agtatgggca
     4501 attttgtcgg agtttccaat gaatcgggag gatttgccat atttagcgac ggcatcaacg
     4561 aataccaact aataaaaggc aagcataacg tcttgacatt aaccttggtc cgtgattttc
     4621 caattgttcg atcaaaaaaa accggcggcc ctattttatc acgcaacgaa aagacagagg
     4681 ttcatttatc tatttatccg cgattatccg attggacgtc cagcgatatt ttaactgagg
     4741 ctataatttt caataacccg ataattatac gacagatgga tcaaacaaat ggatacttac
     4801 cggcgaaaat gcagttttta aaggttaccc cggatacatt gttttattcg gctctcaaac
     4861 ccactgagga tgagaaagat gtagtcctca gactgtataa tccgacccgt aacataatcg
     4921 ttggcgaaat agcaacttat attccaatca aagccgcccg ttaccttacg gtcgaagaac
     4981 atattattgc cccgttggaa atttccgatc cttatcgttt gaaagtaaaa gtttttccac
     5041 agaaaataat gaccttaaaa attatttttg atcgattacc tattaattcg tcaagttctt
     5101 aattttatcc ttttactaaa ggctatttgt cagaattatg ataaaaattt ataataatta
     5161 acgtcaggta gtattaattt agcacgtgtt cgctaatcaa gataaatcaa gtatccagaa
     5221 attagaagaa ttcgtctccg aactaagccg aaatcatatc actgctcaaa aagaagcgcc
     5281 gttagccaaa tttaccacct tcggaatcgg tggcaatgca cgtttactgg tgattgtcga
     5341 aaataccgaa aaactcgaag cggtcatcaa attagccact gcctttcagg taccatttcg
     5401 aattattggt tgtggcagta atttactggt atcggatgac ggatatgacg gtttgatagt
     5461 tgtcaaccga tctaaatcct ggcaaattat tgatgattgt ccagcaaacc ttaaatttag
     5521 ccgaatcatt ccgagatatg atccattgac agcgtctcga acagaagaaa ttcaagcgga
     5581 aaatttatca caaaaaatcg tccgagttga ctccggattt tataataata ccttaatcca
     5641 agaaatgttc gagcaaggct taaccggctt ggagtggttt agcggaatac cgtccagtgt
     5701 gggtggcgct gtttatatga atatgcatgg agcgcacacc tatttcggcg atctggttta
     5761 tcgcgcaaaa ttgattgatg gaatctctcc aaaaatagtg gacaacgctt attttcagtt
     5821 cgattatgat tttagtgttt tgcagaagac cagagaagtt atattatggg tggaattaat
     5881 tctgacgatg ggcaatgtcg aacaggctcg aatcgttgct caagagtgga aaagtagaaa
     5941 atccttccaa ccccgtcgtt ccgccggttg catttttaaa aatctctcac cagaagccca
     6001 aaaacagttt aacctaccta cttcttcagt aggttacttg atagataagg tgctgaactt
     6061 gaaaggattg cggattggtg gcgctgtcat ttctccaaaa catgccgcct ttatcgaaaa
     6121 tgatggaact gcccgggctg atgatgtcta taagttgatt aaaatcatca aagccaaagc
     6181 ccaagagaca ctcggattag aattggaaac tgaggtcgaa ttgcttggga aattttaacg
     6241 ttatgaaagg taattatgtc cagatttatc attgaaggta atcgcaactt atccggtgag
     6301 atcaaggtag ccgggaataa aaatgcggca ttgccgatta tcgccgcgac cgttttgacc
     6361 gatgaaccct gtattctgga aaatttaccc gatattagcg atgttcgttc aatgctagca
     6421 atcctgagcg atttagggaa gtcggtggag atgctatccg ctcataaatg tcaaattacc
     6481 ggatcggtca ataaatcgcg cctaaatgat aaattagcca gtactctacg cgcttccatt
     6541 ctatatttaa gcgttctact ggcgcgtact ggtgaagttg aaatggtacc accgggcggt
     6601 tgtgtaatcg gacggcgtaa agttgaccaa catttcgacg cgttacaggc gttgggagta
     6661 aaagtcaata taattgagca cggctatcgg ctaatattaa aacgccctaa acctaattat
     6721 gtttttctgc gcgaggcttc agtaaccgct actgaaaatg cgatgatgct ggcggcaatg
     6781 acggaaggga aaactaccct tgataatgcg gctggagagc cgcatgtcca agacttagct
     6841 aaagttttag tgaaaatggg cgctaaaatt caaggcgccg gtaccaatcg cttaacaatt
     6901 accggaacca aaaaactaaa aggatttcga catcgcatta tgcctgatca tattgaagcc
     6961 ggcacgttcg cgattgccgc ggcttgcacc ggcggtgaag tcgttattca cgatgccaat
     7021 cgtgaaaatc tgacgatcat caatcttatc cttgaccaaa tgcgagtaaa gttatccttc
     7081 cttgatgaaa aaacgttggt gattaagccg tccaaaaata tcagagctat tccgaaagtc
     7141 caggtaggag tctggccggg tttcccaact gatctgatga gtccgatggt tgtattggca
     7201 acccaagcca gagggacgac tctctgccat gattggatgt acgaatcccg tatgtttttc
     7261 gttgacaaac tggtaacgat gggcgctcaa attgtgcagt gtgacccgca ccgcgtgctt
     7321 gtttcaggtc cgacgcgtct taaagggcag gaaattgtca gtcctgatat tcgggctggt
     7381 attgcgcttg taattgccgc cctaatcgcc cgaggtaaaa gtaaaatcga gaaggctgaa
     7441 ctgattgatc ggggttatga agatatcgtc ccacgctttc ggcaactggg agccgaaatt
     7501 aatcgcgaac aatagtggtt tttctatcga attttattac tttgtggttg ttttcattat
     7561 tttgtaggaa agaagacaat tgattaattt aggcactgat ttttaaagct gaggaaatta
     7621 atgaagccta ttcaatcctt tactgtcaaa ccaattctgc ctaagaaatt ggaagaattg
     7681 cgtgagctgg cttataattt gtggtggacc tggaatgccg atgctcgcga tctctttagg
     7741 cggctggata tggatttgtg ggaagagacg cgccataatc cagtggctct attgaataga
     7801 gtagagcaag atgtactcga aacgcgtgcc caagatgata gctatttgta ccaactgcgt
     7861 cgggtttatg acaaattcaa acagtcctta aactcaccaa cctggttcga aaagagcggc
     7921 aaatacaatt cccacctcaa tattgcctat ttttcgatgg aatatggcat taccgaatgt
     7981 ctggcggact actcaggagg actgggtgta ttagccggcg accaccttaa atcagcttcg
     8041 gaacttggta ttccactcca ggctgttgga ctttcatatc agtacggcta ttttcagcaa
     8101 gtcttaaatg ttgatgatta tcaagaagaa gaatatcctg tcaatgactt ttataattca
     8161 ccgctgactc tggtaaaagg ttctgatggt aaacaattgc tgattaaagt accttttccc
     8221 ggtcgtttgg tttatgctcg gatttggaaa gcccaggttg gtcgtgttcc gctatattta
     8281 cttgatacca atattgccga aaattcagaa gcagataaaa agattacagc tgaattgtac
     8341 ggtggcaata ccgaaacccg aattcagcag gaaattattt taggaatggg tggttttcag
     8401 gctcttcaaa tactcgatgc cacgccattt atttgccata tgaatgaagg gcactcggct
     8461 ttttcgggat tagagcggat tcggaatgtt gtaaaaaaca acggtctgac attttacgaa
     8521 gcgcttgaaa ttattaaagc ggcttcagtt tttaccaccc atactcctgt tcaagccgga
     8581 attgaccttt ttcaccctga tctgataaaa cgctattttg atgagtattg ccgggaagta
     8641 ggaatagaaa ttaatgaact tttagcgttg ggtcgtaacg atccggacaa taattccgaa
     8701 catttttcga tggcaattct agccataaat ttgagtagta agatcaatgc tgtcagtaaa
     8761 ttacatcaac aggttgccca ggaaatgtgg gaatctctct ggccggatat tctcactaca
     8821 gaaattccca ttgactatgt tactaacgga atccaccaag cctcctggat ttcacaagaa
     8881 atgtctgatt tatttgaccg ttatctcgga ccggattgga gacgtgaacc ggcgagtaag
     8941 actatctgga ataaagtaga taaaattccg gacggcgaac tctggcgcac tcatgaacgt
     9001 cgccgggaac gtttagtagc ttatgcccgc agtgaattat atcggcaata tgttactctc
     9061 ggtaaacctc aaaatataat tgcgcgagta aaaagtattt taagcacgga cgctttgaca
     9121 attggttttg cacggcgttt tgccgcatat aaaagagcct atctgctctt cactgatccc
     9181 gaaagattag ctaagatatt gacgaataaa aattttccag ttcagattat tattgctggt
     9241 aaagctcatc cccaagatga agaaggtaaa caggttattc ggcgtatttt gaaactatcc
     9301 caggaagaac cattttatca gtgcgtagtt tttctggaaa attatgatat gaatatggct
     9361 cgttatttgg tacagggctg tgacctgtgg ttgaatactc cgcgtcgtgg tttagaggcc
     9421 tgcggtacca gtggaatgaa agccgtcgct aacggcggtt tacatttcag tacaattgac
     9481 ggctggtggg atgaagtcta tactccggga atcggctggg cgatcggaac tcgtgacatt
     9541 tatgaggata ttgactattg ggatcgtcaa gaagctaata ttctttacaa tgttctggaa
     9601 aacgagatta ttccaacctt tttcgaacgc gataatgatg acttgccccg tgattggatt
     9661 gctatgatta aagcatcaat gaaagccatc tgtcctcaat ataactccaa tcgtatggtt
     9721 tgtgaatacc ttgatcagat ttactatcca gccgccgtga gagccaatga attgtccaag
     9781 gataactatg cagtcgccaa agaattggca cactggaaag agaaaatgcg taaaaattgg
     9841 gacttggttc gcttcctaaa agttacctcc gggtcaatcg aagaattatc ggtaaatagc
     9901 cagctggaga ttcgagcgga gatatatttg gacggtttgc aggttgatga tgtggatgtt
     9961 caggtttatt atggccgcgt tgatcctaac ggcgacatca ttgacgggaa attcagttca
    10021 atgaaatttg aaacaaaagt tgactccaaa aaatacgtgt acgttgataa aatcaataat
    10081 tgggaaagcg gtttgaacgg ctacactatc cggattattc ccaaacatcc ggctttagct
    10141 tatcctttcg aagatggatt aattcattgg tttgatgaat aattgaatct aaattttaag
    10201 cgagctgtta taattttaaa gtaaatatag atggttattg atgaatttaa ttaccaatga
    10261 agttgctcga aaacggatca gtcaaggcga acaagtcgtg gttttaactg gtgctggcat
    10321 atcggcaccc agcggcatcc caacttttcg cggggaaaac ggtttatgga ctaaactatc
    10381 tcctcaggaa ttagccagtt ttgaatcgtt ttatttaaat acagctatgg tgagtgagtg
    10441 gtataaacat cgccgtgaaa ttatcgaagt ttgtcaaccc aatgccggac attatgctct
    10501 tgttgaattg gcgaaaatgg tgtcaaaatt tactttagtt acccaaaata tagacggttt
    10561 gcaccagcga gcgggtagcc gcgatgtatt agaacttcac ggaacgatta tggaaaatta
    10621 ctgcatccga tgcggtcagc aatatagcgc caccgaattt gatgaaattt ataatagttc
    10681 atttgaccat attccacgct gttattgtgg cggtcttatt cgcccgaacg tagtctggtt
    10741 tggtgaatct ttaccgaaac aagcctacga agaggcatat cgtgcttctc aacaagctga
    10801 cctgtttctc tcagtaggga catctgctca ggtacgacct gcggctgatc tgccattaat
    10861 tgctaaagaa aatggtgcct ttttagtaga aattaatatt gttgagacgg tattgactcc
    10921 ttttaccgat ttattcctaa aaggagccgc ggatgaaatt ttgccccaat ttaactggga
    10981 gtaccaatat ctgctgatgc aggtttgata ttaatctgag tttgccagaa aaaatatgaa
    11041 aaataatatc acggtaaaat ctatcgaaac cctgttttta atagggctga tattggtctt
    11101 gtcgaattgt gcctattaca atacctatta caatgccgaa aaatattttc gtgaagcgga
    11161 aaaagaattt aaaacccttc ctgatgaaaa aatcagcccg gcattgaaga aaaaatttga
    11221 taccgcgata gaaaaggcta ataaggtaat cactcaatat ccggagagtc gctgggttga
    11281 tgattcctac tatataatcg caatgtcgca ttattataaa ggcgattacc gaattgcccg
    11341 tcagaagttc gaggattttg ctgtcaaatt tcctcagagc aaattatatc ctgaggcaat
    11401 ggtctggtat ggacgtaatt tgtggaagct ggacgaacgc gagttagctt ttttccagtg
    11461 gaaaaaaatc atgaacctta cggatgatta ttatcttcta gcggagctct atttatcaat
    11521 cggtgaattg tattttaaag acagtgccta tgactcggct ttattttatt atcaaaaagc
    11581 gactgaagtt gggggaagct tcgatattgc cgccgaagct cagttccgta ttgccgagat
    11641 tgacctggct ttaaacagac cacaagaagc cattaaaaat cttaaaaaaa ttgaccagtt
    11701 ttcgccttct ttaatcttac gcgataagat gcaggttctc ctggtgcgaa tttatcgaga
    11761 gtccggtcaa tatgataaag ccattgattt aattaatgaa aaattaaacg atccggctaa
    11821 tgagaaaatc tggggtgatc tggagctaca attgggattg atttatcttg ctcaacaaga
    11881 ttacgatgcc gcaatctctc gattgaccaa ggtaattgag gattataaag gtaaaccggt
    11941 tgcggcggaa gccagctacc acttagccga gttgtatatg actcaaattc atgattatac
    12001 caaagccgct gttcaatatg ataatgtcgt caaaatcgag gttaataccc tgcgggcttt
    12061 tgaatgtcgc aataaatctt ctgaaatcaa acgatttaat actatttata atcgtcttgc
    12121 cgctctaagt gaacaggttg ccgcgattaa tctgaatccg ccgactgaaa gcgatacact
    12181 cggcgagtcg ctcccagttg atcaggagca atcggaattg aagaaagcgc ttgaaaagaa
    12241 aaacacaacc cagaagaaaa caatcgatac actggcggtt ttcaaagaat attacactac
    12301 cctttatgaa atcgctgaaa tattctattt tagttttaat caacccgata gcgcgattta
    12361 ctattttgaa aaagttgcct acgaaatccc ttttaattct ttacgagata aagcactcta
    12421 cagtctttac cgaattgcca gcgaccggca aaatatcgaa aaggctaacg aatatgctaa
    12481 ccagattaag cagtggtttc cggagtcaat ctatttagcc gaaattgaga aacggccgct
    12541 taatacttcc gcccgcgaag tcgaagccga ttcattattg gtaaaagcag aaaatttaga
    12601 ccagactgat ctgcattctg cgataacaat atacgaaaag cttgccaagg aatataccga
    12661 ttgccaagcc gcggagaaag cggttatgta tttagcctat ttctatcatc accgattatt
    12721 tgacttgaat aatgctttaa cctggtacaa atattacctt gacacttatc ccaatgggca
    12781 atattaccaa ataatggcaa atgcttatga tcaactcaag gaaatagaag atgccttagc
    12841 tcaggggagt gaagccgagg aaaatgagcc ccaaaagccg gaaaaggttg aatgagagta
    12901 ttaaaagata ccacggtttt atctaaccgg atggtcgcta ccgataccta tttaatggaa
    12961 tttgcgtttc ccgaaatcag tcagtcggcg caacccggcc agtttgtgaa cttttatttt
    13021 cctgaacgcc cgcaattgtt tccccgtcct tttagcattg cgggagtgaa tcgagaaaat
    13081 ctaaccattc tttataaaaa agtcgggtgc ctgacctcta ttatgagtca atggcaaccc
    13141 agccagaaaa ttcgagttct aggtccttta ggaaatacct tcaatatcaa atataacaat
    13201 catgtattga tagccggtgg agtcggtttg gcaccgctac ttttcctgaa ccaaaaattg
    13261 gctaatttga attacaaaat ctggaatttt gtaggggtgc gttctaaaga tgaattacca
    13321 ccattatcat tattatcggg ggaaaaatat atagcgaccg aagacgggtc agtcgagtat
    13381 tgcggtacag tagtagattg tctccggaat aatctgggag cgattccgca tccatttagt
    13441 gtatatgcct gtggtccgga agcgatgctg agtgctttat ggcatttgtc tcgcgaaaac
    13501 cactttgatt tacaattgtc attggaaaaa ttaatggctt gcggactcgg aatctgccag
    13561 ggatgtgcaa ttccccattc tcatccgtcg gaacaacctc tgcgtttggt ttgtcaggat
    13621 ggtccggtat ttaacgccaa tgaattggac ttaggatgac cgatttacat ataaagttaa
    13681 gagaagattt tattcttagg aatccacttt taacggcttc cggtacttat ggttacggcg
    13741 atgagatagc cgatttgatt aatccggagc tttatggcgg aattgtgacc aaatcaatct
    13801 cgctcgagcc tcgcgttggt aatccgccgc cgcgtattgc cgaagtgacc tccggaatgc
    13861 ttaattcaat cggcttggca aatatcggag ttgataaatt tctggaagag aaggaggaat
    13921 ttctggcggc ttataacggc aaagcaattg tcaatatagc cggtcgcaaa gatgaagaat
    13981 atgttcaggt ggttaaaaaa ttgtccgatc gccaatggat agcggctttt gaaatcaacg
    14041 tttcctgtcc gaatgttagc gagggtggca ttgaattcgg acgagacccg ttaattctgg
    14101 gacaattggt caagaaactg cgtcatgcta ccgataaatt tctaataatt aaactttcgc
    14161 cgaatgtcac tgacatccgg gttctggctt tagccgctca ggaaaacgga gctgatgcaa
    14221 tttcgatgat taacacggtt tacggggcgg cgattgatat tcatacacgt cgtccgaagc
    14281 ttaatactgt agtcgggggg ctttccggac cggcggtcaa gccgatcgcc cttgcccata
    14341 ttattaaaac ttacaaagaa gttgatattc caattatcgg aattggagga atcagttgcg
    14401 gggcggatgt gattgaattt ttattggcgg gagcgcatgc ggttcagatt gggacagcca
    14461 atttttttaa tcctaaagtc tgtggcgaaa tgcttgattt cattgaacaa tattgtacgg
    14521 agaataaaat aagttgtctg cgagatataa tcggacgagt cgaagtctaa gcctaatcgt
    14581 cattttatta ctgtttatga tgttgggctt tatgggttgt gcttccgcgc cgcgttacct
    14641 ctatacttca cctaaaactg gtgcgacaaa tgacgataag aatacgttac tcttgctcaa
    14701 gaattctaac aggaccgccg ttttgactgc ctcatattac ggagcggatt tccatggtaa
    14761 attaacttcc agtggggaag ttttcgatat gaacggaatg acagccgctc ataaatcttt
    14821 gccgtttggg acggttctgc aagtgactta tccaccaact ggtcaatcgg ttactgtggt
    14881 tgttaatgat cgcggaccat ttgtgcccgg tcgcgattta gatttatcgt acgctgccgc
    14941 ccgaaaaatt gggttagtca atgacggtac cggcaaggtg aaagttaaag ttcttaaatg
    15001 gggaaataat gattccccga aacaatgaaa tcaggttttt ttaatgaata acttcgcttc
    15061 tcgtatgcta atcaatataa ttggaattcc agccattttg ctcttgattc atttaggtgg
    15121 aattcctttc gttcttttta taacggtaat ggttttcatc gcgcaggttg aactttatct
    15181 tttgatgcga aaaaaaggaa ttgcgccgct acttttgcct ggtgttttag ccggagtaat
    15241 ttggcaactt gccagttttt atcttccggg cgcactgctg ctttatttac tgataggatt
    15301 ggtagttttg ctcttactag ctagtttggt gcgccagatc aaaaacgtaa cggttgatct
    15361 tgccagtacc ttgttcggtt ttgtttattt accggtgtta acttcgacta taattttaat
    15421 gcgcaatatt cctggtaggg ggcgttcgat tgtacttctt ttgtttgtta caatctgggt
    15481 ttgtgattca ctggctttta taatcggaaa gtggctggga cgtcgcaaga tcgcacctaa
    15541 aattagtcct aataaaacct atgccggttg cattggcgga ctatttggtg ctgtgctgac
    15601 cgtactcatt ttttattttt taggttgggc gccggatttt atgagcataa tgcaattaat
    15661 tatttttggt atactggcgg gagtattcgg acaggctggt gattttgtcg aatcggcttt
    15721 taagcgggac gccgaagtca aggattcatc caatttattg ctcggccacg gtggggtgct
    15781 ggatcgcttt gactcatttt ttatcgccgc gccggttatt tttctttata ttattacaat
    15841 attgtaggaa atctaattgt tctcacacat ggagataaat tgacggagac gaaaatgtca
    15901 ataaacaatg gcaataacga tctggttgaa tatttacata gtttgagtgg tgaaatagat
    15961 ccgaaacagg gttcggtagc tattatctta gccgccggtc atggcaaaag aattaaatcc
    16021 gaacgttcta aaatgttaca cgagatctgg ggtgtgccga ctgtggtgcg cgtcgctaat
    16081 gccgccagta aaggcttggc ttctaacaat cagataattg tcgtgggtat taaagcctgt
    16141 gaagtggcgg aatcagccgg gaaaagagca aatcgcattt ttgtctatca gaccgaacaa
    16201 aaaggtaccg ggcatgccgt gaaagaggca ctttcagcca ttagccagaa tggccacata
    16261 aaagacattt atattttccc gggtgatatg ggattgatta caccagccgt ggtcagcgaa
    16321 cttaaacgcc ggtttgaaca gtccggatgt ggtttagctt tgctgacggg aaattataaa
    16381 ggtgaaccgg aagctaatag ctacggtaga atcatccgcg tcccggcaag aacagctacc
    16441 ggtgaaccat caggagaaga tgagggacgc gttattgaaa ttaaggagca taaagatatt
    16501 ctagcaattc ccgaaagcgg attttatacg actgtgtata atggtaaaaa atatcaattt
    16561 aaacgttcgg aattgctggg aattacggaa tataataccg gcgtttatgc gatgaaattt
    16621 gacctcgctc agcgatttat ccatcaactt actccggata atgtgcaggg tgaattatac
    16681 ttaaccgata taattggcct tttcaatgct aataaaattg aagtggaagc ttatccggtt
    16741 gacgacgaaa gtgcagtaat cggttttaac gtcaagagtg ttttacagga aatggaaaat
    16801 atcgcccgcc gaaaggtgtg gaaacttctg cgggatataa tttatatcga ggatgaaaat
    16861 gattttttca ttgccgatga ggtggttatt cggattttgg aaatggataa aatcaatccg
    16921 ccgctcgaca ttgagatcgg caaaggcgtg catatttcga gtggtgtgca cctcgaacgt
    16981 ggcgtaaaaa ttcgggcggg ctctttactt aatggtaaca tccatctggg cgttggcaca
    17041 gtcgttcagg aaaatgtttc tcttagcacc tatcccaatc aacaaatgac tattggaaaa
    17101 aattgtgtca ttatgcgcgg tgatttgata aaaggtaaca ttacaatcgg cgacaatacg
    17161 agcatcgaat cgagcgtcat tattaccggc agtagcgaat atccaactat catcgggaaa
    17221 aatgttgtca tcaaagggac gacttatata tttgggtcaa ttatcgaaga cgacctgttt
    17281 attgagcatt cggtattgaa atgtaaaagg gtagagcgca cagtccgcaa agatggaaca
    17341 attcagccgg ttcgctgggt tatgccccag ccgcaaggtc tggatatcgt ccgcgatttg
    17401 taacattaac gtagccggta ggctggctag aagaattcat ttaggaccgt acgattgaat
    17461 cgttatacaa atccgttggt ctgcggatag cgtcccgccc aggcgggatt gcggtgttca
    17521 atatccgaca attccagaca tagtttatag ggatgattgg aagttccaca tgtcttggtt
    17581 cataaggaaa tggaattttc gctcctttca aataaggata aatt
//
LOCUS       DAOOMU010000043        17567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_3356_length_17567_cov_16.854328, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000043 DAOOMU010000000
VERSION     DAOOMU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 17567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17567
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3356_length_17567_cov_16.854328"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1980
                     /locus_tag="PLI55_05655"
     CDS             <1..1980
                     /locus_tag="PLI55_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB00354.1"
                     /translation="RTQDGWLHMVYESEGHIWLEHSSDGGNTWILGNNGKPLDNGGGK
                     SPSISYNLPIPSYYSIVLVAFQEGSDIRVRSFAWDGISSYINGPQDWIPSGESPSASL
                     SPNIAFGNDGVFMVVWKKSSGIAYILGILIHLCIGHAGNSQGIISGTNGNSYNPTIST
                     NLLEPGYFDVAWQQQGTPVNGPPPVSIKACRLFFDGEYNCQNGYWGSVSQYNPPQSTI
                     SSSSMKINSNPSIISCPDGPIVSWIADYSGYFTPSQTKAVLHKAWETPFYHVYDAYCK
                     STSISKKDDGSGYYLAYSYMPDIQGWCKYDLAVSSGNLSVTKVVSTNGPYLQLCNGPN
                     KENIYVMAYYPYTIPYYFKKSNSLASAGLSKSVDFLTSDSRGVIIENNESGLYYSFGQ
                     ITVNGNPVNFIPINNDANRVGLNQRTRMERIKNGTNVNLISTDTLNSYLISEPFEMNG
                     NSEIIFGDKFEIGDSTTIKIILGEKGYISFKVDLTDDINGKIIGTIKESKCFRNTLPP
                     RKSSFYKLANSKLGEKRVRIKITVDTNIKEPIFSIVDEHTNADLREGIKKSSIKELSL
                     SNLVTIDEYALHQAYPNPFNPVTTIHYELPKAGNVSLKVYNLLGKEVATLVKGEKQAG
                     RYSVTFDGSRLASGEYIVRMQSGNYSKTIKILLMK"
     gene            2308..2745
                     /gene="rplM"
                     /locus_tag="PLI55_05660"
     CDS             2308..2745
                     /gene="rplM"
                     /locus_tag="PLI55_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006854802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPB00355.1"
                     /translation="MYKTSFPKVTEIKQDWYVVDAEGVVLGRLASKVAAILRGKNKPY
                     FTPHLDTGDYVIVINADKVRLTGRKAEIKTYFKHSGYLGHERFLKYKDVFQASPEEII
                     ERAVKGMLPHNALGRQLFRKLKVYRGPVHPHQAQQPKPLVIEG"
     gene            2753..3145
                     /gene="rpsI"
                     /locus_tag="PLI55_05665"
     CDS             2753..3145
                     /gene="rpsI"
                     /locus_tag="PLI55_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPB00356.1"
                     /translation="MPKLEYVSLGRRKSSIARLRMQTGEGKLIINSRPLDEYFGRESL
                     KRMIIQPFEVCGLTGQFDVNVKVEGGGYTGQAGAIRLAIARALEQYDPELRAKLKPAG
                     FLTRDSRVKERKKYGLRGARRAFQFSKR"
     gene            3186..3890
                     /gene="rpsB"
                     /locus_tag="PLI55_05670"
     CDS             3186..3890
                     /gene="rpsB"
                     /locus_tag="PLI55_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003251698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPB00357.1"
                     /translation="MLMKSGAHFGHLTRKWNPKMKDYVCMTQNGIHIIDLNKTLACLD
                     EATRFIREVVRKGGDVLFVGTKKQAKEIVQREADRCGMFYVVERWLGGTLTNFSTIKR
                     SIRHLQKLEKDKSIGYGENLTKKEKLALERERIKLADLHRGIKDMRKLPDVLVVVDTT
                     YDNIAVQEARRLDIPIIAILDTNADPTIVDYPIPANDDSIQTINLIITELADAVIEAK
                     NSRLVEVPPPVEEAES"
     gene            3914..4519
                     /gene="tsf"
                     /locus_tag="PLI55_05675"
     CDS             3914..4519
                     /gene="tsf"
                     /locus_tag="PLI55_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPB00358.1"
                     /translation="MGITATQVKELRDRTGLGMMECKNALVESDGNIDKAVEILRKKG
                     IAKAEKKADRVAKEGVILSYIHPGNKLGVLIEVNCETDFVAANTDFINFVKDIAMHIA
                     ATNPLSIRREEIDPAILEKEKEIYRAQVRAQNKPENIVERIVEGKVEKFYQENCLLEQ
                     PFVKNPQITVKDYVTSIIAKTGENINIARFVRFQLGESFEK"
     gene            4542..5267
                     /gene="pyrH"
                     /locus_tag="PLI55_05680"
     CDS             4542..5267
                     /gene="pyrH"
                     /locus_tag="PLI55_05680"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPB00359.1"
                     /translation="MASIPYKRVILKFSGEALAGEKQVNFDPDIIRQIAGEIKSAADL
                     GVQMGIVFGGGNIIRGSSGVGKSLDRIKADYMGMLATVINALAMQDALEKLGLRVHVF
                     SAIQMIEVAELMIIRNARKYLDQGEIVIFSAGTGQPFFSTDSGAALRAVEVQAEAIIK
                     ATKVDGVYDKDPEKFNNAQFYDRLDYQTAIEKRLKFMDLTAITICQDNDLPIIVYNMK
                     TPGNLRKVLTGEQIGTLITRSQL"
     gene            5267..5821
                     /gene="frr"
                     /locus_tag="PLI55_05685"
     CDS             5267..5821
                     /gene="frr"
                     /locus_tag="PLI55_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPB00360.1"
                     /translation="MDEVVKDAESRMKKSLDTIQQEMAVIRTGRATPALLDPIKVEYY
                     GTTVPLRQVASIGAPDARLLIVQVFDRNAVTSVEKAIRTAELGLNPIVEGNLLRVPIP
                     QLTEERRKELVKYVHKIAEEGRVAIRNIRRDANEMAKELEKDHEISEDELHNGQEEVQ
                     KLTDKYIKLIDELVKRKEKDILED"
     gene            complement(5984..6844)
                     /locus_tag="PLI55_05690"
     CDS             complement(5984..6844)
                     /locus_tag="PLI55_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPB00361.1"
                     /translation="MWYLVALLSGFFMATADAFSKKSSGQTNALLLAWVREAYALPFL
                     LPILFFIEIPELDATFWIAMAICVAMDLVTTLLYMRAIQIAPLSLTVPYLGLTPLFLL
                     IIPTIVLGERLSVIGIIGVILIAIGTYALQIDRAKSGWLEPWLAIFKNRGSLYMLIVA
                     ICYAITATLGKLAIQHSSPFFMAIVYFGLLAIVYTPFLLITTKGKLAPLINTPSNFLK
                     IGLAMALMAITHYSAINMIQVAYMISIKRLSLLFAILYGVFWFHEQNLRERLIGGIII
                     IAGATFIAFA"
     gene            complement(7018..8784)
                     /gene="dsbD"
                     /locus_tag="PLI55_05695"
     CDS             complement(7018..8784)
                     /gene="dsbD"
                     /locus_tag="PLI55_05695"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HPB00362.1"
                     /translation="MKTRRFLVLVIFLICWNFILANESEILSVKVNLLTPNIQADGKF
                     SAEVNVQINENWHINSNKPSDEFLIPTELRIEPSDEYEISRIIYPKPTLKRLAFAEQP
                     LAIFEGDFNIRLEGKILQTADSLVTVKGSLYYQGCNDQVCLPPTDLPFGLEIPIRPTS
                     INSVKSTLTDITAQSQTTVGNSEKDLDIGNSLAEKGYFLTFILIFLGGLGLNLTPCIY
                     PLIPVTLSYFGGQAVGKKSKTLLLALLYVIGMALINSLLGTLAALSGSLLGSIMTNPI
                     VLIVIAAILVTLALSMFGLYEFGVPNFLMKLAGGSRSGYLGALFMGLTMGIVAAPCIG
                     PFVIGLLTYVASIGKPFIGFLMFFTLSLGLGLPFIVLAFFAAQIDRLPRSGEWMVGVR
                     QIFGFLLIGMALYFIKPLLPTELASIILGLFAIASGIYLILFSKSGNNVRTFVFIKNW
                     LAIIAIIAGTWFLKTEKADRIEMQWQTYSLPAFESALEAKRPILLDFYADWCIPCKEL
                     NKYTFSDPEVIRLSQNFALFQIDLTGKVTPEIQTLLEKYAIKGVPTIIFINRDGREIE
                     DLRLLGFEKADSFIRRMQRALE"
     gene            complement(8954..9595)
                     /locus_tag="PLI55_05700"
     CDS             complement(8954..9595)
                     /locus_tag="PLI55_05700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012670582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB00363.1"
                     /translation="MKFIVFILIALMASALSAQPLKEADIVAIQKKVQALPDEPVDPN
                     EVVVIETDYGKIVIELFANQAPLHAMNFKKLVKAGYYTGTTFHRVIPDFVIQGGDILS
                     RDANPSNDGTGGPGYTIPAEIGQKHLRGSLAAARLPDQVNPQKASSGSQFYICVKDLP
                     SLDRGGYTVFGRVIEGMNVVDKIVAVPRDQRDRPLKDVVMKSVYITDKKSLKK"
     gene            complement(9621..10412)
                     /locus_tag="PLI55_05705"
     CDS             complement(9621..10412)
                     /locus_tag="PLI55_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB00364.1"
                     /translation="MIFCNLGSGSKGNCTYIRHKQKALLIDQGFSLKNLHARMETIAI
                     DEHSVGAIILTHEHSDHLKGVGVFARYHKVPVYLTEQTLNVINPEFFHKVHVCPFRAG
                     DEVTIEDFKVKTFHIPHDAIDPIGIVISAEQYKIAVVTDLGTVSESVLVQIADPDLLF
                     LEANHDLTMLINGPYPLNLKQRIKSRVGHLSNEQSLMLLQRLAGNGRLKHLILGHLSE
                     INNHPSIVRELFENNIDTNLRRYQIEIADQYRPTSIIELTAPHEV"
     gene            complement(10409..>10716)
                     /locus_tag="PLI55_05710"
     CDS             complement(10409..>10716)
                     /locus_tag="PLI55_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPB00365.1"
                     /translation="RENNELVLRRGFNIANGERVVIVDDVVTTGGSLLELIDLATKYG
                     GYILGIGVLIDRSGGKINFGFPFHSLITLEIPTYKPEECPQCLANIPLTTRGRTGKL"
     assembly_gap    10717..10816
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10817..11145)
                     /locus_tag="PLI55_05715"
     CDS             complement(<10817..11145)
                     /locus_tag="PLI55_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPB00366.1"
                     /translation="MKKDENYYLNILKESGAILSGHFLLTSGLHSATYVEKFKILQYP
                     LLCDKLCRGIAKNFENLSPNVVLGAATGGIIISHSVGRALSTRSIFAERENNELVLRR
                     GFNIANG"
     gene            complement(11153..11941)
                     /gene="pyrF"
                     /locus_tag="PLI55_05720"
     CDS             complement(11153..11941)
                     /gene="pyrF"
                     /locus_tag="PLI55_05720"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPB00367.1"
                     /translation="MQFIEKFHECLTEKQTFLCIGIDPDPGKLPSGFAANVDGIYHFV
                     KEIIAATGAYTPAYKFNLAFFEMWGSRGWQMLEKLLSEVPQNILLIGDAKRGDIGNSA
                     KFYAKALFEALPFHAVTLSPYLGTESILPFIADESHGAFVLCVTSNPSSREIQDFGGN
                     QHLYLKVAEIVQQLNQRRNLGLVMGATKPEQLLSVRQKFPNLPFLIPGVGAQGGEIAA
                     AVQVCRTCGVGLINVSRAVLFPDKGKFPENVAETAAYYQEKFKI"
     gene            complement(12016..>14627)
                     /locus_tag="PLI55_05725"
     CDS             complement(12016..>14627)
                     /locus_tag="PLI55_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00368.1"
                     /translation="RANAFRSRDLRSFQKYETQFEAPASINIESITINAKPVSVMQPE
                     SVNPAVILAESLQPGDSVELFIQFTTIIPPGKSKLCSTYSEKSYCLIYFYPRIAEYRR
                     GRWQITGLNELERAPLEFANYSVRFTLPDYFQPAGSLQIDSVVSHNDKNTFIFRSEYL
                     QDIGFILNDRLKVSTIRSRPHNEVKFISRFSANDKSKDKIATEIINDIVDYYAKYLPG
                     MNQQITIYPTVIPGGFTTSRLILIDKTILTEATKLDYLSLYILAREIAHQYLGFNVES
                     ENDLSVSISDGLAGFLANDYLKNRYEFLQQKYKLSDNQLISFNTKVFNVFTSGLEKEN
                     ILQPARASNERANTIFINTQTRFLKSQKLVEMSHYVLGDSLFSQVLAQYQDFIQSDSI
                     DPQVFFKIAEEKSGYEFIALYQNFLHLEQPTDLKIQKVIRSQANNSEHITDVIIKQSS
                     LNYLPLEVNAFDRSGNVYNHRIQPRTGTFDTLTLVTDKPITKIVLDPNRQIWDSNRLN
                     NQYPRSVVFKFLVGLPSIDSYQIFYYPTFDFNNRDIMRFGVKLRGRYWINMRPLFPAQ
                     SLDEWSFGLNYGYKSKTFGYDLSYSTSLLAFLFKPRIYLRWRDYFDLMEGKISTEIYL
                     GDVKYWGFNRVQGYQKLNLGLDYQKVRSLEFLNTNKWETGQSLKPYLDYVNFHNWGNI
                     RHIVHAKFVYGLPVGENHFSFDKLTLDGQVKMRLNHRIWLYQRLFYGNAHGNVPKQEY
                     FYFFGKNVLENQSFESYRLAQGEGDMRGYGDQSLKSYKMLTSNTELRRNLALAGAAMF
                     DCLIFLDSGILPESFSAIDWKQTHFDAGLGVELELLDILKVGMHFPLWVSHPPQSEPS
                     FDLRWVITTDLQL"
     assembly_gap    14628..14727
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14862..14934
                     /locus_tag="PLI55_05730"
     tRNA            14862..14934
                     /locus_tag="PLI55_05730"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14895..14897,aa:Lys,seq:ctt)
     gene            15691..>17567
                     /locus_tag="PLI55_05735"
     CDS             15691..>17567
                     /locus_tag="PLI55_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPB00369.1"
                     /translation="MKQILEKYLANLSDTFNRGDAREESYYKHLDDLIKQCAEIQNIK
                     NIEVTILPKKTDAGNPDFRVWDGQNHITGYIEAKDPSITDLDYIEDSEQLKRYISTFP
                     NVILTNFYEFRLYRDGERIRRVMIGRPVIAQKLKTKPPLENIEQFIELFDLFFSFSLP
                     KVQNARSLAVELAKRTRFLRDEVIAVEMAENGSKGQKQIAGFYEAFKTYLIPTLTAEQ
                     FADLYAQTITYGLFAARTRVTSERDEFNRKIAFDYIPHTIGILRDIFHFISLGDPPKS
                     LQIIVDDIVEILNVADVNKILHEYQRAGKGRDPIIHFYETFLATYDPKIRERRGVYYT
                     PEPVVGYIVRSIHSILKSHFNLADGLASEEVKLLDPAGGTLTFPAEAIRLAADEFKSK
                     YGEGGLHLWIKKHILANFHAFELMMAPYAIGHLKIGFILDELGYKMAEDERFKLYLTN
                     TLEMEEIKEITIPLLSSLSEESHFAEKVKKEQPILVILGNPPYSGISANINKWTERLL
                     KEDLDGAQSYYKVDGQPLGERNPKWLQDDYVKFLRFAQWKIQQSGYGIVGMITNHSYL
                     DNPTFRGMRQSLMKTFDEIYILDLHGNSLKKETTPAGGKDENVFDIRQGVAIALFVKN
                     K"
BASE COUNT         5462 a         3471 c         3656 g         4778 t
ORIGIN      
        1 cgtactcaag atggctggct acatatggtt tatgagagtg agggacatat ctggttagaa
       61 cacagcagtg atggtggaaa tacctggatt ttaggcaata atgggaagcc attagataat
      121 ggcggaggta aatccccatc gatttcttat aatcttccca taccaagtta ttattcaatt
      181 gttcttgttg cctttcaaga aggaagcgat attcgtgtaa gaagttttgc ctgggatgga
      241 atttctagtt atatcaatgg gccgcaagat tggattccga gtggtgaatc tcccagtgcg
      301 tctctctccc cgaacatcgc ttttggcaat gacggagtat tcatggtcgt atggaaaaag
      361 tcgtctggta tagcctatat tcttggtatt ctcatccatc tctgtattgg tcatgcaggc
      421 aattcacaag gtataatatc agggacaaat gggaattcat ataatccaac catatccaca
      481 aatttgctcg aaccgggtta ttttgatgtt gcttggcaac aacaaggaac tccagtaaac
      541 ggtccgcctc ctgtatcaat taaggcatgt aggttatttt ttgatgggga atacaattgt
      601 caaaatggat actggggttc agttagtcaa tataatcctc cccaatctac gatttccagt
      661 agttcaatga aaatcaactc caatccctca ataatcagtt gtccagacgg acctatagtc
      721 tcttggattg cagattatag cggctatttt accccctcgc agaccaaggc agtgttacat
      781 aaagcatggg aaacaccatt ctatcacgtt tatgatgcct actgtaaatc cacctctatc
      841 agtaaaaaag atgatggctc gggctactat ttagcctata gttatatgcc agacatacag
      901 ggttggtgta agtacgactt agctgtcagt agtggaaatc tttcggttac aaaagttgtc
      961 agtaccaatg gtccctacct gcagttgtgc aatggtccaa ataaagaaaa catctatgtt
     1021 atggcttatt atccatatac cattccttac tatttcaaga aatcaaatag cttggcaagt
     1081 gctggccttt ctaaatcagt tgatttttta actagtgata gcagaggtgt aataattgag
     1141 aataacgaat ctggattata ttattcgttt ggtcaaataa ctgtgaatgg aaatcctgtt
     1201 aattttattc caattaataa cgatgctaat cgagtagggc taaatcaacg aacaaggatg
     1261 gagagaataa aaaatggaac caatgtcaat ttgatttcta ctgatactct taattcttac
     1321 ttaatttcag agccttttga aatgaatgga aattctgaaa ttatttttgg agataaattt
     1381 gaaatcggtg actccactac tataaagata atactgggag aaaaaggata tatatcattt
     1441 aaggtagatc tgactgatga tatcaacggt aagataattg gaacgataaa agaatcgaag
     1501 tgctttcgca atactttacc accacgcaag tcatcatttt ataaacttgc taatagtaaa
     1561 ttaggggaaa aaagggtaag aataaaaatt acggttgata cgaatatcaa agaacctata
     1621 ttttcaattg tggatgaaca tacaaatgct gatttacgag aaggaataaa aaaatcgtct
     1681 attaaagagt tatcgttgag taatctagta actatcgatg aatatgctct tcatcaagct
     1741 tatcccaatc cgtttaatcc ggttacaacc atacattatg aattgccgaa ggccgggaat
     1801 gtgtcattaa aagtttataa tcttctcggc aaagaagtgg cgacactggt aaaaggcgaa
     1861 aagcaggccg gaagatattc ggttactttc gatggctcga gactggcaag tggagaatac
     1921 atcgtgagaa tgcaatctgg taattactcc aaaacgataa aaatattatt aatgaagtaa
     1981 tccgcaaatt tctcattgat atttaaaaag cccgattgat agtcgggctt ttctattctc
     2041 taaactaacg taaatgaggc accaagataa aaatacttaa aataatttat aagtgctttt
     2101 taatctttct gtccatatcc agattaggat tggcttgtcc tgattgagaa aagatctatt
     2161 ctggttggct ctgaaagtcg aaagaataat taagaatgga taccatgtcc ctagatcaga
     2221 catgagtcgt ggaaaaattt cttgattata atatcctcaa atcgtaagtt tacccgctat
     2281 ttttgaatta taattaagag ataagcaatg tacaaaacgt cttttccgaa agtgacagaa
     2341 attaaacaag attggtacgt ggttgatgcc gaaggtgtag ttctcggtcg tttagccagt
     2401 aaagtggcgg cgattttacg tggtaaaaac aagccgtatt ttacgccgca tctcgatacc
     2461 ggcgactatg tcatagttat taatgccgat aaagtacgat taactggtcg gaaagcagaa
     2521 attaagactt atttcaagca ttctggttat ctcggacatg aacgcttcct caagtataaa
     2581 gatgtgtttc aagccagtcc agaggagatc atcgagcgag cggttaaagg aatgttaccg
     2641 cacaatgcgc ttggtcgcca gcttttccgg aagctgaaag tctatcgcgg tccagtccat
     2701 cctcatcaag ctcagcaacc taaaccttta gtaatagaag gataagtaac ggatgcccaa
     2761 attagaatat gtgtcattag gtcgtcgtaa aagttcaatt gcccgtcttc gaatgcagac
     2821 cggcgaaggc aaattgatta tcaattcccg tccactggat gaatatttcg gtagggaatc
     2881 tctaaagcgt atgatcattc agccatttga ggtctgtggt ctaactggtc agtttgacgt
     2941 caatgtcaaa gtagagggag gtggatatac cggccaggct ggtgcgatca gattagcaat
     3001 tgctcgggct ttggaacaat atgacccgga gctgagagcc aagttaaaac cggctggctt
     3061 tttaacccgt gactcgcgag tgaaagaaag aaaaaagtat ggattgcgcg gcgcccgtcg
     3121 agctttccaa ttctccaaac gttaaaggca gagagatcag atttatggta aatgtaactg
     3181 tagaaatgtt aatgaaatca ggtgctcatt ttggtcattt aactagaaaa tggaatccca
     3241 aaatgaaaga ttacgtctgt atgacgcaaa acggcatcca tatcattgat ttgaacaaaa
     3301 cgctcgcctg tcttgatgaa gcgacgcgct ttattcggga agtagtacgc aaaggtggcg
     3361 atgtattatt cgtcggaaca aagaagcaag ccaaagaaat tgtccagagg gaagccgatc
     3421 gctgtggtat gttctatgtc gttgagcgct ggttaggtgg aaccttgacc aatttttcaa
     3481 ctatcaaacg cagtatccgg catttgcaaa aattagaaaa agataaatct attggctatg
     3541 gtgagaatct gaccaagaaa gaaaaattag cgctcgaacg tgaacgaata aaattagccg
     3601 atctgcatcg cggtatcaaa gatatgcgta aattgcccga cgtgctcgtg gtagtggata
     3661 cgacgtatga taatatcgcc gtccaggaag cccgccgttt ggacattcca attatagcca
     3721 tcctagacac caatgctgat cccaccattg tggattaccc gattccagcc aatgatgatt
     3781 caatccaaac tattaattta ataatcacgg aactggctga tgctgttatt gaagccaaga
     3841 attcacgatt ggttgaagta ccgccaccgg ttgaagaagc agaaagctaa atagctatac
     3901 agaaaggaaa aatatgggaa tcactgccac ccaggttaag gagttacgag accgtacagg
     3961 cttgggaatg atggaatgca aaaatgcatt ggttgaatca gacggcaata ttgataaagc
     4021 tgtcgaaatt ctacgaaaaa aagggattgc caaagcggaa aagaaagcgg atcgcgttgc
     4081 caaagaagga gtaattcttt cctatattca tcccggcaat aaactgggag tcttaattga
     4141 agtcaattgc gaaactgatt tcgttgccgc caacacagac tttatcaact ttgtgaaaga
     4201 tatagctatg cacatcgcgg ctactaaccc gctatcaatt cgccgggaag aaatagatcc
     4261 agcaatcctg gaaaaagaaa aagaaattta tcgcgctcag gttcgggctc agaacaaacc
     4321 ggaaaacatc gttgagcgca ttgttgaagg taaagtcgag aaattctatc aggaaaattg
     4381 tctgttggaa cagccgttcg ttaaaaatcc ccagattacg gttaaagatt atgttaccag
     4441 cataattgct aaaaccggtg agaatatcaa tattgcgcgc ttcgtccgct ttcagctggg
     4501 cgagagtttc gaaaaataaa taacctctaa caggttaaca aatggcatcc attccttaca
     4561 aacgagtaat cctgaaattt tcaggagaag cgcttgcggg tgaaaagcaa gtaaattttg
     4621 atcccgatat aattcgccaa attgccgggg aaatcaaatc cgccgctgac cttggagttc
     4681 aaatgggaat agttttcggc ggaggtaata tcattcgagg ctcgagtggc gttggcaagt
     4741 cgcttgatcg catcaaagcc gattatatgg gaatgttagc aacggttatt aacgctttag
     4801 caatgcagga tgctctcgaa aaattaggat tgcgtgtaca tgtattttca gccattcaga
     4861 tgatcgaagt cgcggaatta atgataatca gaaatgcccg taaatacctt gaccagggtg
     4921 agatcgtcat cttcagtgct ggaacgggac agcctttttt cagtactgac tccggagcgg
     4981 ctttgcgggc ggtggaagtg caagccgaag ccattatcaa agccactaaa gttgatggcg
     5041 tttatgataa agatccggaa aaatttaaca atgcccaatt ctatgatcgg ctggattatc
     5101 agaccgctat tgaaaaacgt ctgaagttta tggacttgac cgctattacg atttgtcaag
     5161 acaacgactt gccaatcatt gtctataata tgaaaacccc gggtaacttg cgtaaagttt
     5221 taaccggtga acaaattgga acgctaatta cacggagtca attatgatag acgaagtcgt
     5281 caaagatgcc gaatcccgta tgaagaaaag tttagatacg atccagcaag agatggctgt
     5341 aattcgcacc ggacgggcaa ccccggcttt actcgatcca atcaaagtcg aatattatgg
     5401 gacgactgtt ccgttgcgcc aagtagcatc catcggtgct ccggatgccc gccttttgat
     5461 tgtccaagtt tttgatcgaa acgctgtgac cagtgttgaa aaagctattc gaaccgctga
     5521 acttggttta aatcccatag ttgagggcaa tttactaagg gtacctattc cgcaattaac
     5581 cgaagaacgt cgtaaagaat tggtaaaata cgtacataaa attgctgagg aaggccgggt
     5641 tgctatccgt aatatacgcc gcgatgccaa tgagatggcg aaggaactag aaaaagatca
     5701 cgaaatttcc gaagatgagt tacataacgg acaggaagaa gtccagaaac tgaccgacaa
     5761 atatatcaag ctgattgatg aattagtgaa acgcaaggaa aaggacatct tagaagatta
     5821 gtccgacgcc ttaaccaact tcaaaataaa atattataag ccaagaagtc atcaatgttt
     5881 gataggcaaa cgatttgtcc cctgattcga ttttatcatt ttcaaatttc atataaatca
     5941 gaacggtacc actatctctg tcagccttta ccaataaatt aatttaggca aaagcgatga
     6001 atgtcgctcc ggcaattatg attattcctc caatcaggcg ttcccgcaaa ttctgttcat
     6061 ggaaccagaa tacgccatat agaatggcaa atagcaggct gagtcgtttg atcgagatca
     6121 tataagcaac ctgaatcata tttattgccg aatagtgagt aatcgccata agtgccattg
     6181 ccaagccgat ttttagaaaa ttgctcggag tgtttattaa tggtgctaat ttccccttcg
     6241 tcgtaatgag taggaacggc gtgtaaacga ttgctagcaa accgaagtaa acgatagcca
     6301 taaaaaaggg tgaggaatgc tggatggcaa gtttaccgag agtggcggtg atggcataac
     6361 aaatagccac gatcagcata taaagtgagc cgcggttttt gaaaattgcc agccagggtt
     6421 ccagccagcc ggacttggcg cggtcaattt gcagtgcata ggtgccaatt gcaattaaga
     6481 taacgccaat aatcccgatg accgaaagcc gttcgcccaa tactatcgtc ggaatgataa
     6541 gcagaaaaag tggagtcaag ccaagataag gcacggtcag tgataatgga gcgatctgga
     6601 tagcgcgcat ataaagtaaa gtggtgacca gatccattgc gacacaaatc gccatagcaa
     6661 tccaaaaggt tgcatctaac tcggggattt caataaaaaa caaaattggc aaaaggaatg
     6721 gcaaagcata agcttcacgc acccaagcca aaagaagagc attagtttga ccggacgatt
     6781 ttttggaaaa ggcatccgcg gttgccataa aaaatcccga aagtaaagcg acgagatacc
     6841 acatgttatt tcctgattaa aattctgatt tcaagtgcag aatttaatgg acagatgcaa
     6901 actatactat gaaatatagt taatgacgaa tagatataaa taagcctaaa gcattacttc
     6961 tgttgtgaca gattatctta ttttacaata agttcgagaa attaatagtg cgaaatttta
     7021 ttcaagagcc cgctgcattc ggcgaataaa gctatccgcc ttttcaaagc ccagaagccg
     7081 caaatcttct atctcgcgac cgtcgcggtt gatgaaaatt atagttggga cgcctttgat
     7141 ggcatatttc tccagtaaag tctgaatttc cggagtgact ttgccggtta ggtcaatttg
     7201 gaatagggca aagttttgac tcaggcgaat gacttccgga tcgctgaaag tatatttatt
     7261 tagctcttta caaggaatgc accagtcagc gtagaaatcg agtaagatcg gtcgcttcgc
     7321 ctccagagcc gattcgaaag ctggtaggga ataagtctgc cattgcatct caatccgatc
     7381 cgctttttcc gttttaagaa accaggtgcc ggctatgatg gcaataattg ccagccaatt
     7441 tttaatgaaa acgaacgttc tgacattgtt tcctgacttg ctgaaaagaa ttaaataaat
     7501 gccgctggca atggcaaata gccctaatat tattgaagcc aattcagttg gtaacagcgg
     7561 tttgatgaaa tagagcgcca taccgattaa taaaaacccg aaaatctggc gcaccccaac
     7621 catccattct cctgagcgtg gtaggcgatc gatctgagcc gcaaaaaacg ccagaacaat
     7681 gaaaggcaaa cccaacccga gtgacaaggt aaaaaacatt aagaacccga taaacggttt
     7741 accgatcgaa gcgacataag ttaaaagtcc aattacaaat gggccaatgc agggcgcggc
     7801 tacgattccc atcgttagtc ccataaaaag cgcaccgagg tagccactcc gactgccgcc
     7861 tgccagtttc atcaaaaagt taggcacccc aaattcatat aaaccgaaca tactgagtgc
     7921 cagagtgact agaatagccg cgattacgat taaaacgatg ggattagtca taatagatcc
     7981 cagcagactg ccggaaagtg ccgctaaagt tccgaggaga gaattaatta gcgccatccc
     8041 tatgacgtat agcaaagcca acagcagggt tttgctcttt ttaccgactg cttgaccacc
     8101 gaaataactc aatgtgaccg gaattaatgg ataaatacag ggtgtcaaat tcaatcccaa
     8161 gccgcccagg aatatcagga tgaacgtcag aaaataacct ttttctgcca acgaattgcc
     8221 aatatctaaa tccttctcag aattgccaac tgtagtttgc gactgtgcgg tgatatcggt
     8281 tagcgtggat ttgacggaat tgatactggt cggtctaatc ggaatttcca gcccaaaagg
     8341 taaatcagtc ggcggcaggc aaacctgatc attacagcct tgataatata acgagccttt
     8401 cacggttacc aatgaatcgg cggtctggag tatcttgcct tcgagccgga tattaaaatc
     8461 gccttcgaaa attgccagcg gttgttcggc aaacgctaaa cgcttaagag tgggtttagg
     8521 ataaataatc cggctgattt catattcgtc cgatggctca atgcgcaatt cggtcgggat
     8581 aagaaattca tcactcggtt tattagaatt gatgtgccag ttctcgttaa tttgaacgtt
     8641 cacttctgcc gaaaattttc catcagcctg gatattgggt gtcagtaaat tgactttaac
     8701 tgataaaatt tccgattcat ttgccagaat aaaattccag catattaaga aaatgactaa
     8761 taccaagaat ctgcgagttt tcatggttaa tgtttttcta ctattgataa aaaaaagatt
     8821 tcagagttat acaccaatta aaaatggaat gaaaccctcg aaattaaaat ccaggtaatt
     8881 atttaaatta ttcggaattt tttgatcttt aaaaatattc aaatgctggt tataagccga
     8941 caagaaaatc aattcatttt ttaagagatt ttttgtcggt tatatagact gatttcatta
     9001 ccacgtcttt taacggtcgg tcgcgctgat cgcgcgggac tgccacgatt ttatcaacga
     9061 cgttcattcc ttcgatcacc cgtccgaaca cagtgtaacc gccccgatcc agagagggca
     9121 aatccttgac gcagatgtag aattgactgc cactggaagc tttttgagga ttgacttggt
     9181 caggcaaacg cgccgcggct aaactcccgc gtagatgctt ttggccgatt tcggctggaa
     9241 ttgtatagcc cggtccgccg gtgccgtcat tacttggatt ggcatcgcga ctgagaatat
     9301 cgccaccttg aatgacgaaa tccggtatga cacggtgaaa agtcgttccg gtataatagc
     9361 cggcttttac taatttctta aagttcatag cgtggagagg tgcttgatta gcaaacaact
     9421 cgatcacgat tttgccataa tcggtttcaa ttacgacgac ttcgttggga tcaaccggtt
     9481 cgtcaggtaa agcctgcact ttcttctgaa tagcaacgat gtcggcttct ttcagtggct
     9541 gagctgaaag tgcggatgcc attaatgcaa ttaaaataaa aacgataaat ttcataagtc
     9601 tccctaattt aaaaatgatt tcagacttcg tgaggcgcgg tcaattcgat aatcgaggtt
     9661 gggcgatatt gatcggcaat ctcgatttga taacggcgca ggttagtatc tatattattt
     9721 tcaaataact ctcttacaat actcggatga ttattgattt cactcaaatg cccgagaatc
     9781 agatgcttta aacgtccatt accggctaaa cgttgcagta acattaatga ttgttcatta
     9841 gataggtgac caactcgact tttgatgcgt tgtttaagat taaggggata aggtccgttt
     9901 atcaacatcg tcagatcgtg attggcttcc agaaagagta aatccggatc ggcaatttga
     9961 accaataccg attccgaaac tgtaccgaga tcggtcacca ccgcaatttt atactgctcg
    10021 gctgaaatga caatgccaat tggatcaatt gcatcgtgtg gaatatgaaa agttttgact
    10081 ttaaaatctt caatggttac ttcatcacca gctcgaaagg gacaaacgtg gactttgtgg
    10141 aaaaattccg ggttgatgac gttaagcgtc tgttcggtga gataaacggg aactttatga
    10201 tatcgggcaa agacacccac gcctttgaga tggtcggaat gttcatgagt caaaataatg
    10261 gcaccaaccg aatgctcgtc aattgctatc gtttccatac gcgcatgcaa gtttttcagg
    10321 ctaaagccct gatcaattag aagcgccttt tgtttgtgac gaatgtaagt gcagttacct
    10381 ttactgccac tgccgagatt acaaaagatc atagtttgcc tgttcgacca cgagttgtca
    10441 ggggaatatt agccagacat tggggacatt cttccggctt ataagtcgga atctccaatg
    10501 taatcaagga atgaaacgga aaaccgaaat taatttttcc accgctacgg tcaatcaaca
    10561 caccaattcc taggatgtaa cctccatact ttgtggctaa atcaattaat tcaagaagtg
    10621 aaccgcccgt tgtaacgaca tcatcaacga ttacgacgcg ttcgccattg gcaatattaa
    10681 atccacgtcg cagaaccaat tcgttatttt ctctttnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnntcgc cattggcaat attaaatcca cgtcgcagaa ccaattcgtt
    10861 attttctctt tcggcgaaaa tactgcgcgt gctcaaagcc cggccaacgc tgtggctgat
    10921 aatgatgccg ccggttgcgg ctcccaagac gacatttggg ctcaagtttt caaaattctt
    10981 ggcgatgccc ctacaaagct tatcgcaaag taaaggatat tgtaaaatct taaacttctc
    11041 aacgtaggtg gcactatgca agccggaggt cagtaaaaaa tgaccactca gaatggcgcc
    11101 gctttcttta agtatattta aatagtaatt ctcgtctttt ttcatctaaa acctaaattt
    11161 taaatttttc ttgataataa gcggctgttt cggcgacatt ctccggaaat ttacctttat
    11221 ctggaaataa aactgcccga gaaacattaa tcaagccaac gccgcaagtt cggcagacct
    11281 gaacagccgc cgcgatttcg ccaccttgag cgccaactcc cggtatgaga aacggcaaat
    11341 tgggaaattt ttggcgaacg ctcagcagtt gttccggttt agtggcgccc attactagcc
    11401 cgagattccg acgctggttt aattgctgaa caatttcagc cactttaaga tataaatgct
    11461 gattaccgcc aaaatcctga atttcccgac tggaaggatt ggaggtaaca catagaacga
    11521 aagcgccgtg tgattcatcg gcaataaacg gcaagatgct ctcggtacca agataaggac
    11581 tgagagtaac tgcgtggaaa ggtaatgcct cgaacagggc tttagcgtag aatttggcgc
    11641 tgttaccgat gtcaccgcgt ttagcatcgc cgattagcag aatattttgc ggcacctcgc
    11701 tgagcaattt ctcaagcatt tgccagccac gcgaacccca catttcgaaa aacgccaagt
    11761 tgaatttata agccggtgta taagcgccag tggcggcgat tatttcctta acaaaatgat
    11821 agatgccgtc cacattagcc gcaaaaccgg agggcagttt acccggatca ggatcaatcc
    11881 caatgcataa aaaagtctgc ttctcggtga ggcactcgtg gaatttctcg ataaattgca
    11941 taacgcaaaa aattacgttt ttttgtgaga ttaaacaata taagagaagt gtcgaatatt
    12001 agcgttcaat agaaattata attgaagatc ggtggtaatt acccagcgta aatcaaaact
    12061 gggctctgat tgcggaggat gcgataccca taacgggaaa tgcataccaa ctttgagtat
    12121 gtccaacaat tccagctcga cacctaatcc ggcatcgaag tgagtctgtt tccaatcaat
    12181 ggcactaaat gattccggta atataccgct atcgagaaaa atgaggcaat cgaacatcgc
    12241 cgctccagcg agggcaagat tacgacgcaa ttcggtattg ctggtgagca ttttatagct
    12301 cttcagagat tgatcgccat aaccgcgcat atcgccctca ccctgagcta accgataaga
    12361 ctcaaaactc tggttttcca gcacattttt accgaagaaa taaaaatatt cctgtttggg
    12421 tacatttcca tgggcattac cgtagaaaag ccgttgataa agccaaatgc ggtgatttaa
    12481 gcgcatcttg acttgaccat cgagagttaa tttgtcaaaa ctgaaatggt tttcgccaac
    12541 cggtaatcca tatacgaatt tggcatgaac gatgtgtcgg atgttacccc aattatggaa
    12601 attgacataa tccagatagg gttttaaact ttgtccggtt tcccacttat tggtatttaa
    12661 gaactccagg ctacgtacct tttggtaatc taatcccaag ttcagtttct gatagccttg
    12721 aactcgattg aatccccagt atttaacatc tcccagatag atttcggtag aaattttacc
    12781 ctccatcaag tcgaaataat cgcgccagcg cagataaata cgtggcttga ataaaaatgc
    12841 cagcaaagat gtgctgtaac ttaaatcgta tccgaaagtc ttgcttttat aaccataatt
    12901 caaaccgaaa gaccactcgt ctaaactttg agccggaaat aaaggccgca tattgatcca
    12961 gtaacgacca cgtagtttaa ccccaaaacg cataatgtct ctattattga aatcgaaagt
    13021 cggataataa aagatttgat acgaatcaat actcggcaac ccgacaagaa atttaaaaac
    13081 taccgaacga ggatactgat tgttaaggcg attggagtcc cagatttgcc gattgggatc
    13141 gagcacaatt ttggtaatcg gtttatctgt aaccaaggtt aaagtatcga atgttccagt
    13201 tctcggctgt attctatgat tgtaaacatt gccgctgcgg tcaaaggcat ttacttcaag
    13261 cggaagataa tttaacgacg attgtttgat aatcacatcg gtgatatgtt cagaattatt
    13321 tgcctgtgac cggatgactt tttgaatttt taaatccgtc ggttgttcta aatgcagaaa
    13381 attttggtag agagcaatga attcataacc ggatttttcc tcagctattt taaaaaatac
    13441 ttgtggatca atactatcgg attgaataaa atcctgatat tgagccagaa cctggctgaa
    13501 aaggctgtca ccgagcacat aatggctcat ttcaactaat ttttgacttt ttaaaaaacg
    13561 ggtttgagtg ttgataaata tagtatttgc cctttcgttg gaagcccgcg ccggttggag
    13621 aatattttct ttttccaacc cagatgtaaa aacattaaag actttggtat taaatgaaat
    13681 taattggtta tcagacagtt tatatttctg ctgcagaaat tcgtagcgat tttttaggta
    13741 atcgttcgcc agaaatccag ctagaccatc actaatcgag acactaagat cattttcgga
    13801 ttccacatta aaacccaggt attggtgagc aatttctcga gctaatatat acaaacttaa
    13861 ataatcaagt ttagttgcct ctgtcaatat tgttttatca attaatatca gacgagaagt
    13921 tgtgaaaccg ccagggatga ccgtcggata aatcgttatt tgctgattca ttccgggcaa
    13981 atatttggcg taatagtcta ctatgtcatt tattatctca gtagcgatct tatccttgga
    14041 tttatcgttg gctgaaaacc ggctaataaa ttttacttcg ttatgtggtc gagaacgaat
    14101 agtcgaaacc ttcagtctat cattgagaat gaatccaatg tcctgaagat actcggagcg
    14161 aaatataaaa gtatttttat cgttatgact aacaactgaa tcaatttgaa gtgaaccagc
    14221 cggctggaaa taatccggca gggtgaatct aacagaataa ttggcaaatt cgaggggtgc
    14281 gcgctccagc tcattaagtc cagtaatctg ccatctacct cgacgatatt ctgcaattcg
    14341 ggggtaaaag taaatcaggc aatacgattt ttccgaataa gttgaacaga gtttgctttt
    14401 cccgggtggt attatagtcg tgaactgaat gaataattcg accgagtcgc cgggttgaag
    14461 agattcagct aaaattacag ccggattgac cgattccggt tgcattaccg aaaccggttt
    14521 ggcatttatc gtaattgatt caatattgat agacgccggt gcttcaaatt gagtctcata
    14581 tttctgaaaa ctgcgtaaat cacgactgcg aaaggcattg gcgcgaannn nnnnnnnnnn
    14641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14701 nnnnnnnnnn nnnnnnnnnn nnnnnnnaat ataaaagaaa tacatttcat taggaactta
    14761 acttaaaatt aagtcaggat acaatcaagc ttagcgaatc gaatgatgcc tgctattttg
    14821 tttgattctc cggaaaatct taaattaacc cgcttttttc agccccaata gctcaaatgg
    14881 tagagcagcg gactcttaat ccgttggttc aaggttcgag tccttgttgg ggtacacctt
    14941 aaaatacaaa tgtttaaata atttagaacc tcctatctaa tttctattag ccttctaaaa
    15001 atcggtataa tttaactttt caagccggat accggattct tccagttgaa atagtggtta
    15061 gacgacaagc aggtagatat tccggaggga aaaaagcgtt aaaaagttga aaaatagtct
    15121 cctttatccc atatagggaa tagcttttcg atttctattg gtctataata ccttaaccgc
    15181 tgagcggtat ccataatcac tactgcatga ctacgaatcg ctaccgaaca caacactcta
    15241 caatatcttg accacaaagc ggcgggtgcc aatctaacga caatagaatc caaaatagac
    15301 cggaggtttt aaagtattgt agaaattatt ttattgaaaa aaattattta ccctgtagag
    15361 gttaaacttt caataaaaat gggaaaaaac ctgtaattaa aacacattga ctttaactga
    15421 agatttacct aatattgttt tgctacgaaa tatggcagta aattgtttta tattataaga
    15481 ggaaaaagca acttaaatag caatatcaag acctgtttat tctcataaat tttctaaaat
    15541 tacaaacgat tattgaatgg agaaaaatcg taaaaatctc aaaacctaac attgaaaaat
    15601 tatagcacta atttggaaat atctggaaaa tatttttact tgcaatatcg cctaaattgt
    15661 catttaacat ataaaaaaca ttctggttaa gtgaaacaga tactagaaaa atatttagcg
    15721 aatctatcag atactttcaa tcggggtgat gcccgcgagg aaagttatta caaacatctc
    15781 gatgacctga ttaaacagtg cgcggaaatt caaaacatta aaaatattga agtaacgatt
    15841 cttcccaaaa agactgatgc cggaaatcct gacttccgag tttgggacgg tcaaaaccac
    15901 atcaccggtt acatcgaagc caaagaccct tcgattaccg atctggacta tatcgaagat
    15961 tccgaacaat taaaacgcta tatatccacc tttcccaatg ttatcttaac taatttttat
    16021 gaattccgac tttatcggga cggagagcga atccggcgag ttatgattgg acgaccggta
    16081 attgcccaga agctaaaaac caaaccgccg ctggaaaata ttgagcaatt cattgaactc
    16141 tttgatttat tcttttcctt ttctctgccc aaagttcaaa atgcgcgttc gctggcagtc
    16201 gagttagcca aacgcacccg attcttgcgc gatgaggtaa ttgcagtcga aatggcggaa
    16261 aatgggagca aaggtcagaa gcaaatagcg ggcttttatg aagcctttaa aacttacctg
    16321 ataccgactt taaccgcaga acaattcgcc gatctttatg ctcaaactat cacatacggg
    16381 ttgttcgccg ctcggacaag agttacatct gagcgggatg aatttaatcg taaaattgcc
    16441 ttcgattata ttccccatac tatcggaatt ttgcgggata tattccactt tatctcgctc
    16501 ggagatcctc cgaaatcact ccaaatcatc gtggacgata ttgtcgaaat tctaaatgtt
    16561 gccgacgtca ataaaatctt gcatgaatac cagcgcgccg gaaaaggccg ggatccaatt
    16621 atccattttt atgaaacttt tctggctacc tacgatccta aaatccgtga acggcgcgga
    16681 gtatattata ccccggaacc ggtggtcgga tatatcgttc gttcgattca ttccatttta
    16741 aaatcgcatt ttaatctggc ggacggtctc gccagtgaag aagtaaaact gctcgaccca
    16801 gccggtggta cgctcacctt tcccgccgaa gccatccggc tggcggctga tgaattcaaa
    16861 agcaaatacg gcgaaggcgg tttacattta tggataaaga aacatatcct cgccaatttt
    16921 cacgcttttg agctgatgat ggcgccctat gccatcggtc acttgaaaat tggttttatt
    16981 cttgacgaac tcggttacaa aatggctgag gatgaacgct tcaaacttta tctgaccaac
    17041 acgctggaaa tggaagaaat aaaggaaatt acaattccct tactttcttc gctgagtgaa
    17101 gaaagtcatt ttgcggaaaa agtaaagaaa gaacagccga tcctagtgat tcttggtaat
    17161 ccaccttata gcggcatttc cgccaatatc aataaatgga cggaacgact gctcaaagaa
    17221 gaccttgacg gcgcccagag ttattacaaa gtagatggtc agccgctggg agaaaggaat
    17281 cctaaatggt tgcaggacga ttatgtcaaa tttctacgct tcgcgcaatg gaaaatccag
    17341 caatccggtt acggcatcgt ggggatgatt accaatcaca gttatctgga caatcctact
    17401 tttcgcggaa tgcgtcaaag tctaatgaaa accttcgatg aaatttacat tcttgatttg
    17461 cacggcaata gcctgaaaaa agaaacaact cccgccggtg gcaaagacga aaatgtgttt
    17521 gatattcgtc agggcgtggc aattgcgtta tttgtgaaaa ataaaaa
//
LOCUS       DAOOMU010000044        16798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_3596_length_16798_cov_20.866571, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000044 DAOOMU010000000
VERSION     DAOOMU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 16798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16798
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3596_length_16798_cov_20.866571"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLI55_05740"
     CDS             <1..207
                     /locus_tag="PLI55_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPB00370.1"
                     /translation="EETHVEIVVYDLMGREVWKSAKTHYSAGTYSVVWNGVNQSGQPV
                     GTGMYIVRLKSLRYSATQKVLLMK"
     gene            complement(411..1763)
                     /locus_tag="PLI55_05745"
     CDS             complement(411..1763)
                     /locus_tag="PLI55_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HPB00371.1"
                     /translation="MSNSMLQSISQKMQKASQIDPELYVKYNVKRGLRNEDHTGVLVG
                     LTRIGDVVGYRTENGQVIPIEGQLLYRGYQLDDLVKGFQAENRHGFEEICYLLLAGEL
                     PNRSELQYFSDYLAKQRELPENFTNQMLINLVSGDMMNMLARAVLALYTNDPQPEDRS
                     VENLISQSLSLIAKFPTIVAHSYNAYRHAYQQKTLSIRHPHKDLSIAENFLYMLKGNR
                     YTKLEADMLDLALVTHAEHGGGNNSTFTLRVTSSTGTDTYSAIAAAIGSLKGPYHGGA
                     NRKVMEMMTDIKKSLKNWDDRNAVADYLMKILKKEVGDRVGKIYGFGHAVYTLSDPRV
                     KILKSKAADLATEKNRLEEFHLYNLVEELAPQVFADFKGREKSKIVCANVDFYSGFVY
                     SCIGIPPELYTPIFAVSRLAGWCAHRLEELNPQSRRIIRPAYKNVTPEREYVNIAERR
                     "
     gene            complement(1783..3033)
                     /gene="icd"
                     /locus_tag="PLI55_05750"
     CDS             complement(1783..3033)
                     /gene="icd"
                     /locus_tag="PLI55_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009791360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPB00372.1"
                     /translation="MSNEQIVFANGSLQVPNFPLIPFIEGDGIGAEICRPTLRLIDVA
                     VNRAYGANRRIIWREVLAGDKSLNQNGEALPAATLQDLNQYVIGLKGPLRTPVAEGRR
                     SLNVILRQTLDLYACVRPLHWFPGVPTPVRRPELLNVVIFRENTEDLYTGIEFMRGSL
                     EAGLLEDFLIGKLKAPEPRFPGENSLGVKPVSKPGSERLIRAAINFAIKYKLPSVTLV
                     HKGNIMKYTEGGFRNWGYELAEREFAESCITAKSGAPAGSLTSGSGDKVLIKDMITDA
                     FFQDILLNPARYSVIATLNLNGDYISDLAAAMVGGIGLAPGANINFETGRAIFESTHG
                     TAPDIAGHDIANPSSLIMSGVMMLEYMGWSEAANLLQKAMANLFIRQIGTVDIFGNVN
                     GARCVGTTEFVDLLEDEILKINIK"
     gene            complement(3051..5315)
                     /locus_tag="PLI55_05755"
     CDS             complement(3051..5315)
                     /locus_tag="PLI55_05755"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPB00373.1"
                     /translation="MDVDFINNFYREFAERVSRLRSELGRALSFAEKIIYGHLFSEQP
                     QSKLKPRESYVNLRPDRVAMQDATAQMALLQFINAGRRQVAVPTTIHCDHLIVAKDDA
                     VTDLKRARRENREVYEFLQTAAYKFGIGFWPPGEGIIHQIILENYAFPGGLMIGADSH
                     TPNAGGLGMLGIGVGGADAVDVMAGLPWELKMPGLIGVELTGRLNGWASPKDVILKLV
                     GMLGTKGATGMVIEYFGDGAANLSCTGKATICNMGAECGATASIFPYDAKMAEYLTLT
                     GRAEVAKAATAAFDSILQADPEVYQTPQKYFDRVIKIDLDNLEPYVNGPFSPDMAVPL
                     SQLRDHIKKSGYPAHLSAGLIGSCTNSSYEDLSRAADVARQANRHKIATRAKLLVNPG
                     SQWVKLVCEKAGFLNEFRTLKAQILANACGPCIGQWERDDCEPGIKNSIITSFNRNFA
                     GRNDGNQQTHTFLASPEIVLALTLAGDLNFNPLTDFLISEDGKRYQLEPPEGEELPAV
                     KIQTSRTPARIIHKPDITIKINPLSQRLRLLEPFPAPTSQSFQNLLLLMKVKGKCTTD
                     HISPAGEWLKYRGNLDRISDNLLLGARNAFTGKLGETLNQLTGEYAKVADVARSYQSH
                     QKLSVIVADENYGEGSSREHAAMEPRYLGVVAVIARSFARIHETNLKKQGVLALTFTQ
                     FDDYERVREDDEFSIKGLEKFAPERPLDLIIQHKNGESETIQLQHSYSQLQIEWFWAG
                     SALNYLKLNNPQKL"
     gene            complement(5380..6483)
                     /gene="leuB"
                     /locus_tag="PLI55_05760"
     CDS             complement(5380..6483)
                     /gene="leuB"
                     /locus_tag="PLI55_05760"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPB00374.1"
                     /translation="MEVKKIAVLAGDGIGPEIMNEALKVLNAVQQKSEIRFEFREALV
                     GGAAWEKYGQHLPEETLQICRNSDAILFGAVGGPVEEQFSPKWRGVEVNALLGLRKTF
                     DLYANLRPAKLFPALVFASPLRQDIAEKGFDILVVRELTGGIYFGQPKGRRGNGAEEV
                     AFDTMIYSRREIERIAHVAFKAARQRRRHVTSIDKANVLTTMVFWREVVTEVAQQYPD
                     VTWESMYVDNASMQLVKNPAKFDVMLCGNMFGDILSDEAAAIAGSLGMLPSASLAESL
                     FGLYEPGGGSAPDIAGQNSANPIAQILSAAMMLTYSFGMNEAAMAIEQAVSDVLQAGY
                     RTADIWQTGCHKIGTAEMGDRIVTELLKKINPE"
     gene            complement(6484..6972)
                     /locus_tag="PLI55_05765"
     CDS             complement(6484..6972)
                     /locus_tag="PLI55_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPB00375.1"
                     /translation="MQGNAHVYARDHINTDEIIPARYLNIDREAELAEHAMEDIDVNF
                     IKKVKPGDFIVSGDDFGCGSSREHAVWALRGAGIKAVIANSFARIFYRNCINNGFLAI
                     ECPGAVRKISDGDRLEIDLAGGKIVNLTKNQTYQFQPLPDFARKIIEAGGLMEQIKKE
                     LA"
     gene            complement(6982..8244)
                     /gene="leuC"
                     /locus_tag="PLI55_05770"
     CDS             complement(6982..8244)
                     /gene="leuC"
                     /locus_tag="PLI55_05770"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPB00376.1"
                     /translation="MGATITEKILATHSGREHVKPGELVDCKIDTVMCHDVTTTPAIE
                     LLEKYAIPKVFDPARIVIMPDHFVPNKDIKSAEMVARIRRWAQAQGITQFYDIGCHGV
                     CHALLPEQGFVTPGKTIVCGDSHTCTHGALGAFATGIGSTDLAATLATGKLWFKVPES
                     MRFVVSGRLADGVYAKDIILKIIGQIGVDGALYRALEFCGEVIDELSVEGRMTICNMA
                     IEAGGKSGIINPDAKTLEYVQARTTGEFTVYRSDSDAEYYCTIEIDVSDLEPLVALPH
                     LPSNVKPVREVSAIPIDQAYLGSCTNGRIEDLRIAAQILKNRTVARGTRMIVVPATTE
                     IWKQANREGLLDIFMSAGATVSTPTCGACLGGYMGILAAGERCISSTNRNFVGRMGSP
                     KSEVYLASPATVAASAITGKITDPRPYL"
     gene            complement(8266..9810)
                     /locus_tag="PLI55_05775"
     CDS             complement(8266..9810)
                     /locus_tag="PLI55_05775"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPB00377.1"
                     /translation="MAKRIIIFDTTLRDGEQSPGASLTASEKLIIARQLANLGVDVIE
                     AGFPIASEGDFEAVKQIAQTVEGPIICGLARTNEIDIDRCWEAVKYAAKPRIHTFIAT
                     SNIHLEKKLHKTGAEVIEMAYSAIRHAKQYCTDVEFSPEDCARTSLEYMCEIVQAALE
                     AGATTINIPDTVGYAEPEEFAERIRYLIDKVPAIRQAVISVHCHNDLGNAVANSLAAI
                     RAGATQVEGCINGIGERAGNAALEEVVMNLVTRKDYFDAETGINTREIFKTSRLVANL
                     TGLSIPRNKPIVGQNAFSHEAGIHQHGVLSSRQTYEIMTPEDVGWEGENLVIGKHSGK
                     HAVAALLEAEGYRPDPEQLNQITNKVKDLADKQKEVAAEDILAIARDVTMQLPEEEQH
                     IQLEEFTVTTGSGLTPTASVRLVVNGERKIGAATGDGPIDAVSNAIWSVIGPSLQLVE
                     YSLKAITGGTNALADVSIKLADQKGNIFAARAVNEDVIKASAMGIIKGINKALSSKQK
                     IHPHTV"
     gene            complement(9823..11403)
                     /gene="cimA"
                     /locus_tag="PLI55_05780"
     CDS             complement(9823..11403)
                     /gene="cimA"
                     /locus_tag="PLI55_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPB00378.1"
                     /translation="MNRKIQIYDTTLRDGTQAETISFSADDKLHIAKRLDEFGIDYIE
                     GGWPGSNPKDMEFFKLARETEFRHARLVAFGSTKRAGNAIEDDQNIHNLLKAETLAVA
                     IFGKTWILHVEKALRITPAENLELIRESVAYLKSKGKEVIYDAEHFFDGYKDNADYAI
                     QTLQTAQSAGADLITLCDTNGGALPSEISRIISEVRRKITAPLGIHTHNDSGLAVANS
                     LAAVEAGCVLVQGTINGYGERTGNADLCSIIPNLQLKAGYQCIPSENMKQLTPLSRYV
                     SEIANLAHPNDLPYTGRSAFAHKGGVHVSAVMKDPRSYEHIEPESVGNTRRVLVSDLS
                     GQSNIIFKAQELNIDLERHQDKIPEIVAKLKELEHLGYQYEAAEGSLELLISKIKSEY
                     HDFFKLKGFRILLEKNEDGEQRSEATIRVEVNNTIEHTAAEGDGPVNALDNALRKALT
                     KFYPEIAEMKLSDYKVRVLNPQQATAAKVRVLIESSSDGETWGTVGVSENIIEASWQA
                     LTESISYYLMKKRQKNSK"
     gene            complement(11407..12408)
                     /gene="ilvC"
                     /locus_tag="PLI55_05785"
     CDS             complement(11407..12408)
                     /gene="ilvC"
                     /locus_tag="PLI55_05785"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPB00379.1"
                     /translation="MSVKLFYEKDVKSDSLKKKTIAIIGYGSQGHAQAQNLRDSGYKV
                     IIAEMPSSPNYALAVKHGFKPVSAAEASEQADVIQILTPDEVQKRVYEQEIKQNLTKG
                     KALVFSHGFNIHFKQIVPPADVDVYMVAPKGPGHLVRRQYEIGAGVPSLFAVYQDVTG
                     QARNLALEHACGIGAGRAGMIETTFREETETDLFGEQAVLCGGVSELIKAGFQTLVDA
                     GYQPEIAYFECCHELKLIVDLIYEGGLAGMYYSVSDTAEYGGMTRGPYIIDCETKKRM
                     QTLLRAIQTGSFAKDWILENQAGRPTLNAYRQEHQELLLEKVGRELREMMPWLKKKG"
     gene            complement(12426..12911)
                     /gene="ilvN"
                     /locus_tag="PLI55_05790"
     CDS             complement(12426..12911)
                     /gene="ilvN"
                     /locus_tag="PLI55_05790"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPB00380.1"
                     /translation="MIEKHTIIVTVVNRVGILARITGLLAQRGYNIESAIAAPTEEKD
                     IYKIILVVKETSENIEQITKQLNKLIDTIKVTDISHKDNYIVREYIILKVGLTPRTRA
                     EVLDLMRVFGAKPIDLATDHLIMELAGHVRKIDRFIELLKPFGIEEYVRSGEFAMTEY
                     N"
     gene            complement(12908..14644)
                     /gene="ilvB"
                     /locus_tag="PLI55_05795"
     CDS             complement(12908..14644)
                     /gene="ilvB"
                     /locus_tag="PLI55_05795"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPB00381.1"
                     /translation="MKGAEILIDALINEGVDTIFGYPGGVVIPIFDVLYRTDKIRFIL
                     TRHEQGATHAADGYARATGKVGVCLVTSGPGATNTITGIANARMDSIPIVVISGQVDT
                     ANIGTDAFQETDMIGLTRSITKHNYLVKSVEELPIVLKEAFYIARTGRPGPVAIDIPV
                     NVSNAQLKNYQYPSEVTLPGYKPVTNGNPKQIEKLAQAIKTAKKPLLYTGGGIISANA
                     EPELLALLDKTNIPIVITLMGLGSIPSDYPTFLGMPGMHGTMAANLALNECDLLIGIG
                     VRFDDRITGKREQFARQATVAHIDIDPAEIGKVIKPHIPVVGDAKKILSELVKKVSPR
                     PKDEWNERTLAWKNQYPMTYDQDAEEAILPQYVIDRVSALADPDAILVTDVGQHQMWA
                     AQYYRHRKPRSFISSGGLGTMGFGLPAAMGAAVGRPGHTVITISGDGSIQMNIQELAT
                     CSINKIPVKVIILNNSYLGMVRQWQELFWDEHYSQTCLKRSPDCPPQCTGPRKDCPSV
                     YIPDFVKLAEANRIYGYRATKPEEVDDVLKQGLSQPGPVIMEFVVKSIENVYPMIPSG
                     KTISDVIPGRQS"
     gene            complement(14644..16338)
                     /gene="ilvD"
                     /locus_tag="PLI55_05800"
     CDS             complement(14644..16338)
                     /gene="ilvD"
                     /locus_tag="PLI55_05800"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPB00382.1"
                     /translation="MRSNQIKKGIERAPHRSLLRALGLTEKDFAKPFIGIANSYTEII
                     PGHAKLNVVGEIVKDAIIKAGGMPFIFNTIGVCDGIAMGHDGMRYSLQSRELIADSVE
                     TMVKAHCFDGLVCIPNCDKITPGMLMAAVRLDIPTIFVSGGPMKAGRTAGGKALDLIS
                     VFEGIGAYQEGKISESELYEIECCACPGWGSCSGLFTANSMNCLCEALGIALPGNGTI
                     LAEDERRIELYKAAGARIVELVRSNLCPSQIITRASIDNAIVMDMAMGGSSNTVLHLM
                     AVANEAGIDYSINRMDEISRKTPTICKVAPSSPYHIEDVDAAGGMSALLNEIFRENEF
                     VNKDCLTVTGKTIAENVAGKRIIDDSVIRHLENPYSPTGGLAIMTGNLAPEGSVVKYA
                     GVSDKMKVFEGPAVIFESQEEACEGILSGKVKAGDVVVIRYEGPRGGPGMQEMLSPTS
                     YIMGRGLGESVALITDGRFSGGTRGACIGHVAPEATVGGPIALLKNGDRIRIDIPQKS
                     LEVLLSDSELQSRRAQWQARPPRITHGVMARYAEMVASASQGAVLKYNSRIKGEKL"
BASE COUNT         4374 a         4165 c         3673 g         4586 t
ORIGIN      
        1 gaagagacac acgtcgagat cgtggtttac gatctgatgg gtcgcgaagt ctggaaatca
       61 gccaaaacac attactccgc cggaacctat tcagttgtct ggaatggcgt caatcaatcc
      121 ggccagccgg tcgggactgg aatgtatatc gtcagactga aatcactcag gtattctgct
      181 actcagaaag tattacttat gaagtagttc cgtttaaaaa tcctccctga agtggttttc
      241 tactacgagg gttttatttt tacccatcga atgcttctaa accgatttta tccgccgggc
      301 aaaattggtt gctgatacag ccaaagttca tcctgtaaac aacggcgtat cgcaatcaat
      361 aaccacaaat gactaccaac gaatgttgag taaccattga ataatgtatt ttatcttctc
      421 tctgctatat tcacgtactc acgttctgga gtaacatttt tatatgccgg tcgaatgata
      481 cggcgcgact gcggattgag ttcctccaga cgatgggcgc accaaccagc cagtcggctg
      541 acggcaaaaa tcggagtata aagctccggc ggaattccga tacaagaata gacaaatccc
      601 gagtaaaagt caacattggc gcagacgatt ttagattttt cgcgtccctt gaaatccgcg
      661 aagacctgag gcgcaagttc ttcgactaaa ttatagagat gaaattcctc aagacgattt
      721 ttttctgtcg ccaaatctgc agctttgctc tttaaaattt tcactcgcgg atcagaaaga
      781 gtataaacgg catgcccaaa tccatagatt ttaccgacgc gatccccgac ttcttttttc
      841 aatattttca ttaaataatc agccacagca ttcctgtcat cccagttttt caaacttttc
      901 ttgatatccg tcatcatttc cattactttg cgattcgcgc cgccgtgata aggtcctttc
      961 agagaaccaa tagccgccgc gattgccgag taggtatcgg tcccggtaga actggttact
     1021 cgtaaggtaa aggtcgagtt gtttccgccg ccatgttcag cgtgggtaac taaagctaaa
     1081 tcgagcatat ccgcttccaa tttggtatag cgattgcctt taagcatata tagaaaattt
     1141 tcggctatgc ttaaatcttt atgcgggtga cgaatagata aggttttttg ctgataggca
     1201 tgtcggtaag cattatacga atgggctact atggtcggaa atttggcgat tagacttagc
     1261 gattgactga taagattttc tacacttcga tcttccggtt ggggatcgtt ggtatataac
     1321 gccagaaccg cccgagcgag catattcatc atatcaccac taaccaaatt aatcagcatt
     1381 tggttagtga aattttctgg caattcgcgc tgtttagcta gataatcgga aaaatattgc
     1441 agttcggaac gattcggcag ttcgcccgcc agaagcagat agcagatttc ttcaaagcca
     1501 tgccgatttt cggcctgaaa gcctttaacc aaatcatcca gttgatagcc ccgatagagc
     1561 aactggcctt cgatcggaat tacctgacca ttttccgtac gatagccaac tacatcgccg
     1621 atgcgggtta atccgaccag aactcccgtg tgatcttcgt tacgcaggcc tctttttaca
     1681 ttgtacttga catataattc cgggtctatc tgactggctt tttgcatttt ttggcttatg
     1741 gattgtaaca ttgaatttga catattaagt tccttttaat cgctacttta tatttatttt
     1801 caggatttca tcttctaata aatcaacgaa ttcggtggtg cctacacaac gagcgccgtt
     1861 gacattaccg aaaatatcaa cagtaccaat ctgacgaata aataagtttg ccatagcctt
     1921 ttgcaaaagg ttggcagctt ctgaccaccc catatattcc agcatcatta ctcccgacat
     1981 aatcagtgag gatggattgg cgatgtcgtg accggcaata tcaggggctg tgccgtgagt
     2041 tgattcaaaa atggcgcggc cggtttcaaa attgatattc gcacccggcg ccagaccgat
     2101 tccaccgacc attgccgccg ccaaatcgga gatataatca ccgttcaaat tcagagtagc
     2161 tattaccgaa taacgagccg ggttgagcaa tatatcctga aaaaacgcat ccgtgatcat
     2221 atccttaatc agaactttgt cgccggatcc tgaggttaat gatccagctg gagcgccact
     2281 cttagccgtg atgcaagatt ccgcaaattc acgttcagcc agctcatatc cccaattgcg
     2341 aaagccacct tccgtatatt tcatgatatt gcctttatga accagcgtaa ccgaaggcaa
     2401 tttatattta atggcaaagt taatcgccgc tcgaataagc cgctccgaac cgggtttaga
     2461 aaccggtttg actcctaaag agttttcgcc agggaagcga ggctccggcg ctttcaattt
     2521 accaatcagg aaatcttcca gcaggccagc ttctagcgag ccgcgcataa attcgattcc
     2581 ggtatagagg tcttccgtgt tttctcgaaa aataacgacg ttcaagagct ccggtcgtct
     2641 gactggcgta ggcacacccg ggaaccaatg cagaggtcga acgcaggcat aaagatcaag
     2701 agtttgccgt aaaataacgt tcaaagagcg gcgcccttca gcaactggcg tccgtaaagg
     2761 acctttcaag ccgataacat attgattcaa atcttgaagg gttgcggctg gtaatgcttc
     2821 accattttga ttcaaagatt tatcgcctgc taaaacttcg cgccagatga tgcgccggtt
     2881 agcgccataa gcccgattga cagccacatc aatcaaccgc agagtgggtc ggcaaatttc
     2941 agctccaatc ccgtcgcctt caataaaagg aatcagcgga aaatttggca cctgaagcga
     3001 accattagcg aaaactattt gttcattgga catctaaagc ctccctaaaa tcagagcttc
     3061 tgtggattgt ttaatttaag atagttcaag gcactgccag cccaaaacca ttctatttgt
     3121 aactggctgt agctatgctg taattggatt gtttcacttt ctccattctt atgttggata
     3181 atcaaatcca gcggacgttc gggagcaaat ttttcaaggc ctttgatact aaattcgtcg
     3241 tcttcccgaa cacgttcata atcatcgaat tgggtaaagg ttaaagccag gactccctgc
     3301 ttcttcaaat tggtttcgtg aatgcgggca aaagagcgcg caattaccgc tactactccc
     3361 aaataacgcg gctccatcgc ggcatgttcg cgcgatgagc cttcaccata attttcatcg
     3421 gcaactatta ccgataattt ctggtgggat tggtagcttc tagcgacatc agccaccttg
     3481 gcatattcgc cggtcaactg gttaagggtt tcgcccaatt ttccggtaaa ggcattacgg
     3541 gcacccagca gaaggttatc ggaaattctg tccagattgc cgcggtactt cagccattca
     3601 ccagctggtg aaatatgatc ggtagtgcat ttacccttta ctttcattaa aagcaacaaa
     3661 ttctggaaag actggctggt tggcgcggga aatggttcca gtaaacgtaa cctctgggag
     3721 agcggattaa ttttaatcgt aatatccggt ttatgaataa ttcttgccgg agtgcggctg
     3781 gtctggattt ttacggctgg cagttcctca ccttcaggtg gttcgagctg gtaacgcttg
     3841 ccgtcttcgc tgattagaaa gtcagtcaaa ggattaaagt ttaaatcgcc agccaatgtt
     3901 agagccagca cgatttccgg cgaagccaga aaggtgtgag tttgctggtt gccatcattt
     3961 cgtccggcaa aattgcgatt aaaactggtg ataatagagt tttttattcc cggctcgcaa
     4021 tcatcccgtt cccattgacc aatacacggt ccgcaggcat tcgctagaat ttgagctttt
     4081 aacgttctga attcattcag aaaaccggct ttttcacaaa ccaacttgac ccattgggaa
     4141 cccggattga ctaataattt tgctcgggta gcgattttat gacgatttgc ttgacgggcg
     4201 acatctgccg ctcgggacaa atcttcatag gaagaattgg tgcaagaacc gattaagcca
     4261 gctgataaat gagctgggta gccgcttttc ttaatgtgat ctcttaactg tgaaagcggt
     4321 actgccatat caggtgaaaa aggtccgttg acatacggtt ccagattatc caggtctatt
     4381 ttgatcactc gatcgaagta cttttgggga gtttgataga cttctggatc agcttggaga
     4441 attgagtcga aagccgccgt agcggctttt gcgacctccg ctcttccggt cagagtcaaa
     4501 tattctgcca ttttagcatc ataaggaaat attgaggcag tcgcgccgca ttctgctccc
     4561 atattgcaaa ttgtggcttt gccggtacag gaaagattag ccgcgccatc gccaaaatat
     4621 tcgataacca taccggtagc acctttagtg cccagcatgc cgactaattt caagatcaca
     4681 tccttgggtg aagcccagcc attcagccgc ccggttaact cgactccgat caagccgggc
     4741 attttcaatt cccacggcaa gccagccatc acatctacgg cgtcagcccc accgacgcca
     4801 atgccgagca ttcctaagcc accggcatta ggcgtatgcg aatcggctcc gatcatcaaa
     4861 ccacccggaa aggcataatt ttccagtata atttggtgaa ttatgccttc accaggcggc
     4921 cagaaaccga taccaaattt gtaggcggct gtttgcaaaa attcatagac ttcccggttt
     4981 tctcgtcgcg ccctttttaa atcagtaact gcgtcgtctt ttgccacaat tagatggtca
     5041 cagtgaatag tagtcgggac ggctacttgt cggcgaccgg cattgatgaa ctgcaataat
     5101 gccatttgag cggtcgcatc ctgcatagcg acccgatcag gcctcaggtt cacatacgat
     5161 tcacggggtt tcaatttcga ttgaggttgt tcagaaaaca agtgcccgta tataattttt
     5221 tccgcaaagc ttagcgcccg gccaagttcg cttcttaatc ggctaactcg ttcagcaaat
     5281 tcgcggtaaa aattgtttat aaaatcaaca tccaattaaa atttactcct cattctgata
     5341 acattcctta atgaacgttt ctctataatt ataaatattt tattcaggat taattttctt
     5401 taataactcg gtaacgatgc ggtcgcccat ttcagctgtg ccaattttat gacaacctgt
     5461 ctgccagata tcagccgtgc gataaccggc ttgcaggaca tcggaaaccg cttgttcgat
     5521 agccattgcg gcttcattca ttccaaaaga ataggtcagc atcattgccg cacttaaaat
     5581 ctgggcgatt ggattagcgc tattttgtcc ggcaatgtcc ggcgctgaac cgccacccgg
     5641 ttcgtataac ccaaaaagac tttccgccag gctggcgctg ggcaacattc ccagactccc
     5701 ggcaattgcg gcggcttcat cggaaagaat gtcgccgaac atatttccac aaagcataac
     5761 atcgaactta gcgggattct tgaccaattg catagaagca ttgtcaacat acattgattc
     5821 ccaggtaacg tccggatact gttgagcgac ttctgtgacc acttcccgcc agaaaaccat
     5881 cgtcgtcaat acattggctt tatcaatgct ggtaacgtgt cggcggcgtt gacgagcggc
     5941 tttgaaagcc acatgcgcga tccgctcaat ctcgcggcgg gaatagatca tagtatcgaa
     6001 agcgacttcc tcagcaccat tgccgcgtcg gcctttcggc tggccaaaat aaattccgcc
     6061 ggtcagttcg cgcactacca atatatcaaa tcccttctcg gcaatatcct ggcggagagg
     6121 tgaggcaaat actaaggcgg ggaaaagttt agccggacgc agattggcat ataaatcaaa
     6181 ggttttgcgc aaaccgagga gggcgttcac ttccacgccg cgccattttg ggctaaactg
     6241 ttcctctact ggcccgccga cggcgccgaa aagaatcgca tcactattac ggcaaatctg
     6301 gagagtttcc tccggtaaat gctgaccgta tttctcccaa gccgctcctc ccacgagcgc
     6361 ttcgcggaac tcgaatcgaa tttcgctttt ctgttgaaca gcgttcagca ctttcagagc
     6421 ttcattcatt atttccggac cgatgccatc accggctaac acggcaattt tctttacttc
     6481 catctaagca agttcctttt taatctgttc cataagccct cccgcttcga taattttccg
     6541 ggcaaaatcg gggaggggtt gaaattgata agtctggttc tttgtgagat tgacaatctt
     6601 gccaccggct aagtcaattt cgaggcgatc gccatccgaa attttacgca ccgcgcccgg
     6661 gcattcaatc gctaagaaac cattattgat acagttacgg taaaaaatac gagcaaacga
     6721 attggctatt acagctttaa tcccagcccc acgcaacgcc caaaccgcat gttcgcggga
     6781 actgccacaa ccaaaatcgt cgccactgac aataaagtcg ccgggcttga ctttttttat
     6841 gaaattgaca tcaatatctt ccatagcgtg ctcagctaat tcggcttctc ggtcaatatt
     6901 caggtagcgc gccggaataa tttcatcggt attgatgtga tctctggcat aaacgtgagc
     6961 atttccttgc ataatcaact cctacaaata tggccgcggg tcggtgattt tgccagtgat
     7021 cgccgaagcc gctaccgtcg ccggactggc taaataaact tccgatttcg gactgcccat
     7081 ccgtccaaca aaattgcggt tggtcgagct gatacaacgt tcgccagccg ccagaattcc
     7141 catatatccg ccgagacagg cgccacaagt cggcgtgctg acggtagctc ctgccgacat
     7201 aaaaatatcc agtaatcctt cgcggtttgc ctgtttccag atttcagtgg tagccggcac
     7261 gacgatcatc cgtgttccgc gcgcgacggt acgatttttc aaaatttggg cggcgatccg
     7321 caaatcctcg attcgaccat tggtacaact gccgagataa gcctgatcaa ttggaatagc
     7381 ggacacctcg cgtacaggct tgacattgct gggcaaatgc ggcaaagcca ccagcggttc
     7441 taaatcggaa acatcaattt cgatggtaca atagtactcg gcgtcgctat cgctacgata
     7501 aacagtaaat tcgccggtag tgcgagcctg gacgtactcc agtgttttag catcgggatt
     7561 aataatgccg gatttgccac cggcttcaat cgccatattg caaattgtca ttcttccttc
     7621 gaccgatagt tcatcaatta cttcaccaca gaattccaga gcgcgataca gcgcgccatc
     7681 cacaccgatt tgtccgataa ttttcaggat aatgtctttg gcgtaaacgc catccgccag
     7741 gcgtcctgaa acaacaaacc gcatcgattc tggcactttg aaccagagtt taccagtcgc
     7801 taaagttgcc gccagatcgg tgctaccaat accagtagcg aaagcgccca gtgcgccatg
     7861 cgtacaagta tgactgtcgc cgcatacaat tgttttcccg ggcgtcacaa aaccctgctc
     7921 cggcagaagg gcatgacaga cgccatgaca cccaatatcg taaaattgag taatgccctg
     7981 agcctgtgcc cagcggcgaa tgcgagccac catttccgct gacttgatgt ctttattagg
     8041 tacgaagtgg tcaggcataa taacgatgcg cgccggatcg aagactttgg gaatagcgta
     8101 tttttccagc agttcaatcg ccggtgtggt agtaacatcg tgacacatta ccgtatcaat
     8161 tttgcaatca accaattcac caggcttgac gtgttcccgg ccgctatgcg ttgctaaaat
     8221 tttttcagtt atggttgcgc ccaatttatt ctccaaatta ttaattcaga ccgtgtgggg
     8281 atggattttc tgtttactac taagcgcctt atttatgcct ttgataattc ccatagcgct
     8341 ggctttaatg acgtcctcgt taacggcgcg ggcggcaaag atattcccct tctggtcagc
     8401 caatttgatc gaaacatcgg ctaaagcatt cgttccgccg gtgatggctt tcagactata
     8461 ttccaccaat tgtagagagg gccctattac gctccagata gcattgctga cagcgtcaat
     8521 cggaccatcg ccggtagccg cgccgatttt cctttcgcca ttaaccacca agcgcaccga
     8581 ggcggttggt gtcaagccac taccggtcgt gacggtaaat tcttccagtt ggatatgttg
     8641 ctcttcttcc ggcaattgca tagttacatc gcgggcaata gccaggatat cctcggcggc
     8701 gacttctttt tgtttgtcag ccaagtcttt gactttgttg gtaatttgat tgagctgttc
     8761 cggatcaggg cgatagcctt ccgcttccag taaagccgcc acggcatgtt taccggaatg
     8821 tttgccaatc accaggtttt cgccttccca accgacatct tccggcgtca taatttcata
     8881 agtctgccga ctgctcagaa cgccatgctg atggataccg gcttcatgtg aaaaagcatt
     8941 ttgtccgacg atgggcttat tacgcggaat gctcagaccg gtcaaattgg ccaccaaacg
     9001 actggtcttg aagatttcgc gggtattgat cccagtctcg gcatcgaaat aatctttgcg
     9061 ggtaaccagg ttcataacga cttcttcaag tgcggcattt ccggcgcgtt cgccgattcc
     9121 attaatacag ccttccactt gggtagcacc agcccggata gcggcgagcg aattagctac
     9181 ggcattgccc agatcattat gacaatggac gctgataacc gcttgccgaa ttgccggcac
     9241 cttatcaatc aggtaacgaa tccgttcggc aaattcctcc ggttcagcat agccgaccgt
     9301 atcgggaatg ttaatggttg tagcaccggc ttccagggcg gcttgcacaa tttcacacat
     9361 atattcgagg ctagtccggg cacaatcttc gggtgaaaat tcaacatcgg tgcaatattg
     9421 ctttgcgtga cggatagctg aataagccat ttcaattacc tcagccccgg tcttatgcaa
     9481 ttttttctca aggtgaatat tagaggtagc gatgaaagta tgtatgcgcg gtttggcggc
     9541 atatttaaca gcttcccagc aacgatcaat gtcaatttcg ttagtgcgtg ccagaccgca
     9601 gataatcgga ccttctacag tttgcgctat ctgttttacg gcttcgaagt cgccttcgga
     9661 ggcaattgga aatccggctt cgatgacatc cacgccgaga ttggctaact gccgggcgat
     9721 gatcaatttc tcagaagccg tcaaagatgc gccgggactc tgttcgccat cccgcagagt
     9781 cgtatcaaaa attataatgc gtttagccat attaacctcc gtctatttgg aattcttttg
     9841 acgttttttc attaaataat aagagatgct ctccgttaga gcttgccagc tggcttcgat
     9901 gatattttcg gaaacgccga cggtgcccca ggtttcacca tcggaggaag attcaatcag
     9961 cacacgaacc ttggcggctg tcgcttgctg gggattcaat acgcgcactt tgtaatctga
    10021 caatttcatt tcggcaattt cgggatagaa tttagtcagg gcttttcgga gagcattatc
    10081 gagggcattg accggaccat cgccttcagc cgcggtatgt tcgatggtat tgtttacctc
    10141 cacacggatc gtggcttccg agcgttgttc gccatcctca tttttctcga gcaggatgcg
    10201 gaatcctttc agcttaaaaa aatcgtgata ttcggattta attttgctga tcaaaagctc
    10261 caatgaacct tctgcggctt cgtattgata accgagatgc tcaagttcct ttaatttggc
    10321 aacaatttca ggaattttat cctgatgccg ttcgagatca atattaagtt cttgcgcctt
    10381 gaagataatg ttgctctggc cggataaatc cgagaccagc acgcgacgag tattgccgac
    10441 gctttcgggc tcgatgtgtt cataactgcg cggatctttc atcacagcac tgacgtggac
    10501 tcccccttta tgggcaaaag cgcttctacc agtgtaaggc aaatcattag ggtgagccag
    10561 attagcaatt tcactgacat atcgcgaaag cggagtcagc tgtttcatat tttccgacgg
    10621 gatacactga taaccggctt tcaactgtag attcgggata atcgaacaca ggtcggcgtt
    10681 gccggtgcgt tcgccatagc cgttgattgt accctggacc agaacgcaac cggcttcgac
    10741 agccgctaaa ctattcgcca ctgccagacc actgtcatta tgagtgtgaa tacccagagg
    10801 cgcggtgatc ttccggcgca cttcggagat gatgcgactt atttctgaag gtaaagcacc
    10861 accgttcgta tcacacaaag tgattaaatc agcgccagcc gattgagcgg tttgcaaagt
    10921 ttgaatagca taatcggcat tatctttata accgtcgaag aaatgctcag cgtcgtagat
    10981 tacctctttg cccttgcttt ttaaataggc tacactttca cggatcaact cgagattttc
    11041 cgccggtgta atacgcagag ccttttcgac atgcagaatc caggttttgc caaaaatcgc
    11101 aactgccaga gtttccgcct taagcaaatt gtgaatattc tgatcatctt ctatggcatt
    11161 accagcccgt ttagtcgaac caaaagctac taggcgggcg tggcgaaatt cagtctcgcg
    11221 agccagtttg aaaaattcca tatctttggg attcgagccc ggccagccgc cttcgatata
    11281 atcaatccca aattcatcca gtcgcttagc gatatgcaac ttgtcgtcag cagagaaact
    11341 gatagtttca gcctgggtgc cgtcgcgcag agtcgtatcg taaatctgga ttttacggtt
    11401 catattttat ccttttttct taagccaggg catcatctcg cgcagttccc gacctacttt
    11461 ttccagtaat aattcctgat gttcctgacg gtaggcattg agcgtgggac gcccagcctg
    11521 attttctaaa atccaatcct tggcaaatga gccagtctgg atggcacgca gtaaagtctg
    11581 catccgtttc ttggtttcgc aatcaatgat gtacgggccg cgggtcattc cgccatattc
    11641 cgccgtatca ctgacggaat aatacatacc agctaacccg ccttcataaa ttaaatcaac
    11701 gatcaattta agttcgtgac aacattcgaa gtaagcgatt tcgggttggt aaccggcatc
    11761 caccagagtc tgaaatccgg ctttaatcaa ttccgaaacg ccaccgcaca agaccgcctg
    11821 ttccccgaaa agatcagtct cagtttcttc gcgaaaggtt gtttcgatca ttccggctct
    11881 gccggcaccg ataccacaag catgttccag agctaggttc ctcgcttgac cggtgacatc
    11941 ctgataaacc gcaaatagcg acggtacgcc agcaccaatc tcgtattgac ggcgaaccaa
    12001 atgaccggga cctttcgggg caaccatata aacgtccaca tcggccggcg gtacaatttg
    12061 tttaaagtgg atattgaatc cgtgagaaaa aaccagggct ttgcctttag tgagattttg
    12121 tttgatttcc tgttcgtaaa cgcgcttctg aacctcgtcc ggcgtcagaa tttgaatgac
    12181 gtcagcttgt tcactggctt cagccgcgct gacgggtttg aaaccatgct taacggctaa
    12241 ggcataattt ggcgaactcg gcatttcagc aataataact ttgtacccgc tgtcacgcaa
    12301 gttttgggct tgagcgtgtc cctgactgcc atagccaatt atggcgatgg tttttttctt
    12361 cagggaatca cttttaacat ctttctcata aaacaatttg actgacatag ctaactcctt
    12421 ttttattaat tatattctgt catagcaaat tcaccactgc ggacatattc ctcgattcca
    12481 aatggcttaa gtaactcgat aaaccgatcg attttgcgga cgtgtccggc taattccata
    12541 atcaaatgat cagtggctaa atcaatcggc ttggcaccaa aaactcgcat caaatccagc
    12601 acttctgccc gagtgcgagg agtaagaccg actttcaaaa ttatatattc gcgcacaata
    12661 tagttatctt tatgggaaat atcggtcacc ttgattgtat caatcagctt attgagttgc
    12721 ttggtaattt gctcgatatt ctcactggtt tctttgacga ctaaaatgat tttataaata
    12781 tccttttctt cagttggagc ggcgatagcg ctttcgatat tataaccgcg ctgagccaat
    12841 aagccagtaa ttcgggccaa aataccaact cgattgacta cggtaacaat gatagtatgt
    12901 ttttcgatca tgattgcctc cctggaataa catctgaaat cgtcttgcca gatgggatca
    12961 ttggatagac attttcgatg cttttcacga cgaattccat aataaccgga ccaggctggc
    13021 tcagtccctg ctttaataca tcatccacct cttcgggctt agtagctcga tagccataaa
    13081 tccgattagc ttcggcaagt ttgacaaaat ctggaatata aactgatggg caatccttgc
    13141 gtgggccagt acattgtggc ggacaatcag gactgcgctt caaacaggtc tgtgaataat
    13201 gttcgtccca aaacagctcc tgccattgac gcaccatacc cagataggaa ttatttaaaa
    13261 taattacttt gaccgggatt ttattgatgg aacaggtagc taattcttga atattcatct
    13321 gaatactgcc atcacctgaa atggtaatga cggtgtgccc gggacgacca actgccgcac
    13381 ccattgcggc tggcaagcca aatcccatcg tccccaatcc accggatgag ataaacgagc
    13441 gcggtttacg atgccgataa tattgagccg cccacatctg atgctggccg acatccgtaa
    13501 caagaatcgc atccggatca gccaaggcac taacccggtc aattacgtac tgcggcaaaa
    13561 tagcttcctc cgcatcttga tcgtaagtca tcgggtactg atttttccag gcaagagtgc
    13621 gttcattcca ttcgtctttc ggacgcggac taactttttt caccaattca ctgagaattt
    13681 tcttagcatc acctacgacc ggaatgtgcg gcttgataac ttttccaatt tcagctggat
    13741 caatgtcaat atgagctacg gtggcttgcc tggcaaactg ttcgcgtttg ccagtgatgc
    13801 gatcatcaaa gcggactcca attccgatta acaaatcgca ttcgtttaaa gccaagttag
    13861 cggccattgt accgtgcata ccgggcattc cgaggaaagt cggataatcg gatggaattg
    13921 aacccaaccc catcagagta atcacaatcg gtatattagt tttatcgagt agggcaagca
    13981 gttccggctc agcattagcc gagatgatcc ctccgccggt gtaaagcagt ggttttttag
    14041 cagttttaat ggcttgcgct aacttttcga tttgcttggg attgccatta gtcaccggtt
    14101 tgtaccccgg cagagtaact tcagacggat attgataatt tttcagttgg gcattggaaa
    14161 cattaaccgg gatatcaatt gccaccggac cgggtcgccc ggtgcgcgca atgtagaaag
    14221 cctctttaag aacgatcggc aattcttcta cgctttttac taaataatta tgcttagtga
    14281 tggaacgagt caatccgatc atatcggttt cttgaaaagc gtcagtgccg atattagcgg
    14341 tatccacctg accactgatg actacaatcg ggatgctatc cattcgggca ttggcaattc
    14401 cagtgatcgt gttggtggcg cccggaccgg aagttaccag acagacaccg actttgccgg
    14461 tagcgcgagc atatccatcc gcggcgtgag ttgcaccttg ttcatggcgg gttagaatga
    14521 atctgatttt atcggttcgg tacagtacat caaaaatcgg aataactacg ccgcccggat
    14581 aaccaaatat agtatcaacc ccttcattaa ttaaggcatc aattaaaatt tcggcgcctt
    14641 tcattacaac ttttctcctt ttatgcgtga gttatatttc aagaccgctc cctgactggc
    14701 agaagccacc atttcggcat aacgagccat tactccatgc gttatgcgtg gcggacgcgc
    14761 ctgccattgg gcgcgacgac tttgcaactc tgaatcagaa agtaaaacct ccagcgattt
    14821 ctggggaata tcaatgcgaa tacgatcacc gtttttaagt aaagcaattg gtccaccgac
    14881 tgtggcttcc ggggcgacgt ggccgataca agcgccgcgt gttcccccgg aaaatcttcc
    14941 atcggtaatc aatgccacac tttcacccag accacgcccc ataatatagg aagtgggcga
    15001 aagcatctcc tgcattcctg gtccaccgcg aggtccttca tagcgaatga cgaccacatc
    15061 accggctttg actttgccgg atagaattcc ttcgcaagcc tcttcctggc tttcaaaaat
    15121 aactgccggg ccttcgaata ccttcatttt gtcggaaact ccggcatatt taacgacgct
    15181 cccttccggc gctaaattgc cggtcattat cgccaaacca ccagttgggg aatagggatt
    15241 ttccagatgg cggataaccg agtcgtctat aatacgctta ccggcgacgt tttccgcaat
    15301 ggtttttccg gtcaccgtca aacaatcctt attgacaaat tcgttttccc ggaaaatttc
    15361 gttcaacaga gcgctcatac cacccgccgc atctacatct tcaatatgat aaggtgaaga
    15421 aggcgcaact ttacatatcg tcggcgtctt ccgtgaaatt tcatccatac gattaatcga
    15481 atagtcaatg ccggcttcat tcgcaaccgc catcaaatgt aaaactgtgt tcgacgagcc
    15541 gcccattgcc atatccatca cgatcgcatt atcaatagaa gcgcgcgtga ttatttgcga
    15601 tggacacaag ttggaacgca ccagttctac aattcttgcc ccagccgctt tataaagctc
    15661 aatccggcgc tcatcttcag ccagaatagt accgttgccg ggcagggcta ttccaagcgc
    15721 ttcacacaaa caattcatag agttagcagt gaaaagtccc gaacaacttc cccagccggg
    15781 gcaggcacag cattcaattt cgtaaagctc agactcgctg attttgcctt cttgataagc
    15841 gccaatcccc tcaaagaccg aaatcaaatc gagggctttc ccgccagctg tcctgccagc
    15901 tttcatcggc ccgcctgata cgaagatcgt cggaatatcc agccgaacgg ccgccatcag
    15961 cattcccggt gtaattttgt cacagttcgg aatgcaaacc agaccatcga aacaatgtgc
    16021 tttcaccata gtttctacgg agtcggcgat taattcgcgg ctctgcagac tgtagcgcat
    16081 accatcatgt cccatcgcaa tcccgtcgca aacgccaatc gtattaaaaa tgaatggcat
    16141 accaccggct tttataatgg catctttaac tatctcacca acgacattta gtttggcatg
    16201 cccgggaatg atttcggtat aagagttggc aatcccgata aacggtttgg caaaatcctt
    16261 ttccgttaag ccgagcgccc gcaagagtga gcgatgaggt gctcgttcga ttcctttttt
    16321 tatctgatta ctgcgcattt agatattcct tttattttgt tataaaaaaa ccccttctcg
    16381 atttagaagg ggttttagaa actgtttatt tttttctacg tctaaattac cccttcacct
    16441 ctagctagga taataatgct ccacggtagt acgatgcaat gtatagattt taagtaactc
    16501 ataattaata ctcccaaata taaaatattt ctagcttgta agtcaacaat tttatttaat
    16561 caatgctata aattttttaa aatgtgaaat ctaacttaac aaaatctcgt ttgttataaa
    16621 gattaattcg ctaatccagg taattggtaa atcaccagcc gtctttaatt tgtgacattg
    16681 gtagtttaaa ataatctttg taaaatacga ttgtggggat aattgaaaag taaattaaaa
    16741 agttacctaa aatgcagtga tgcatcatct aaaccaagag ggggacaatg aaagaact
//
LOCUS       DAOOMU010000045        16258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_3779_length_16258_cov_22.744121, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000045 DAOOMU010000000
VERSION     DAOOMU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 16258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16258
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3779_length_16258_cov_22.744121"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..486)
                     /locus_tag="PLI55_05805"
     CDS             complement(52..486)
                     /locus_tag="PLI55_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00383.1"
                     /translation="MKQDEQQNDKRTLLFLRRLIQNVIGFLQRNYKLLITWAIIIIAV
                     IVGIHFRVDKGIIVVLVMIFGLFAEAFSGLIALLATVPVIGPIVAKVLTLPIFWIFNA
                     LGYFVSLVAIKRGYKKEVINYRFVTMVFLTGFAVGFIIAKLL"
     gene            complement(521..1315)
                     /locus_tag="PLI55_05810"
     CDS             complement(521..1315)
                     /locus_tag="PLI55_05810"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPB00384.1"
                     /translation="MLLAIDIGNTNATVGLFQENDLKGCLRINSHPVRGADYYGRRIE
                     SLLKKQGIAINVIDGIVIGSVVPRLTEPFKQMAQTFFHIEPLLINCNLNLGCCLLVDN
                     PAEVGADRICNAVATGVKYHCPSIVVDLGTATTFDVLDKEGNYIGGAIAPGLITGARD
                     LFRKAAQLYPINLTFPPRVIGKNTTAHMQSGILFGHLAMIEGMIRRIGVEINQPDIFV
                     IATGGFSNVIAQQSPLIQASDPYLTLDGLRLIYGMNLPQTSKSQSR"
     gene            complement(1318..2100)
                     /locus_tag="PLI55_05815"
     CDS             complement(1318..2100)
                     /locus_tag="PLI55_05815"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPB00385.1"
                     /translation="MNSFRLVNLPIPTEILKFPVVHFAEIDSTNAYLLGRHSHPSGTI
                     VWADFQSAGRGRLGRSWITPPNEALLFSIYLKIHHNETPLFIYSFLTAVGVLEGLQTL
                     VTPAWLALKWPNDVLLKNLKVCGILVQSKTSSGDNADVVIGIGVNLNQNSDFFIPDLP
                     FAGSLYSITGKKYEREDVLAKLIFAIDQNLVALNGNGTVPILEKWRKYCPFIGQKIKV
                     NDGQKIHSGLFYDISADGGLILQVGRTYRTFHAAEVTIVRET"
     gene            complement(2105..4258)
                     /locus_tag="PLI55_05820"
     CDS             complement(2105..4258)
                     /locus_tag="PLI55_05820"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPB00386.1"
                     /translation="MSNIKRLFFLATCLLTLNFRLLAAEGMYPPDQIPTKIKGLKVKP
                     ADIYQPDGGGLAGAVLLFGGGTGSFVSADGLVLTNHHVAFSAIQKNSTDEQNYLAQGF
                     YARTKAEELPAPGYEASLMIGFEDVTEKILKSFTPQMTPEERTRALERAIAQLEAESE
                     DSANGLTGKVVAMLDGASYYLYHYRKFRDIRLVYAPPQSIGNFGGEVDNWMWPRHTGD
                     FSFMRIYCAPDGKPAEYSEKNVPYQPKAFLPISTRGLNPDELVFILGYPGRTYRNVTS
                     YSVAYNQNLVYPLRIRIFQEIINELEDESQKSPEVDLLLSSRLKGFYNGLKNNQGLLA
                     GFKSENILGQKKLVEKELVQKIAGKPAWQEQYGNILPEIQKAYDEYYTGFERDMYIEY
                     LRYVTVLADALTIEKWSREKAKPESEREYGFFDYQIARTKRDFKVRRIGYYAPADARI
                     LKLLLNHLAEFSSAERPAFLRELIQDQPADSAKAVIAAYVDSAFAHTRLTDADECLAM
                     FDLSAESLLDRQDPLITLAVAVNGELERIEKDQKTLSGKMLILKPQYFSALKNVLGKT
                     VLPDANRSLRFTYGNVEGYQPRDAVFYQPQTTLAGVIAKHSGIEPFNVPERLIELATS
                     RDYGRWIDRELNDVPVAFLSSCDITGGNSGSPVLNARGELVGCAFDGNWEALTNDWQY
                     NPALSRAISVDVLYVLFILDKYSSADELIEELELR"
     gene            complement(4283..5035)
                     /locus_tag="PLI55_05825"
     CDS             complement(4283..5035)
                     /locus_tag="PLI55_05825"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HPB00387.1"
                     /translation="MGKLAGTIIGGGIGWALFGPLGALIGAYIGNVVTNESGVQKKRG
                     KAEPGATFATYGDTRAGDFAVAMLSLFTYISKSDRNVKSSEIQYVKKFLVDQFGTRNA
                     QDLLYLYREILGKDYNIQDIAHQIRDNMDYYSRMELLHVLFGIAAVDGDLHTAESQAI
                     REISDALGISARDYETIRAIFSGSANRSYEILGIKPDDDVEIIKKAYRDLAVKYHPDK
                     VANLGPEFQKLAEEKFKSINDAYQTIRKERGF"
     gene            complement(5164..6285)
                     /locus_tag="PLI55_05830"
     CDS             complement(5164..6285)
                     /locus_tag="PLI55_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00388.1"
                     /translation="MRRTLSRIVVLTLFAVATIMAQEFSMDQIKELAATNGKLYLQPL
                     ATGFGTAMNSGLYNTAGTHGTLGFDIQLKTGVGLAPASKASQTTYQFDLSALDDIAFN
                     IEKNSQSYSITLNPEEIFSDCTTPNILGGEAEPFRVNQPYVQGEIESQLATQLRAQYP
                     LLTDAQINAMIAQMNPEIEDAINNIQVKSIPGMKSIKALEDLKTVPFPFPILQASVGL
                     PMGIEPSIRIIPKFKIPGDIGEAIGEISALGGGLKLDVDRWIPIPMFPLDIAVQGYYQ
                     QMEIGDLLSANSTAFNLMVSKTILMLTPYVGVGKESSKFTASYKMEDGTKIKFDLKGK
                     NDTRFTAGLNIKIAVISINAEYSMGEYNAAGLGVGFALR"
     gene            complement(6353..>8332)
                     /locus_tag="PLI55_05835"
     CDS             complement(6353..>8332)
                     /locus_tag="PLI55_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPB00389.1"
                     /translation="GQTNSIIKLDNDLAIVPSSFKFSKTPDAVGQKVSFSLKIKNVGR
                     NPAENFSIKIYLDQNQDSCRQADELCWRQDINQSLKSDSTLIVTGKITAERAGRSLYL
                     AELEYSLDENLDDNYAGTDLVVAFPAGCLTLNEFLAYPTSNQVEFIECINVSDETIDL
                     SGWMLGNRYLKAEISGGKVPAGGYLILSGDSACFNYFKTDNAAVIIPGKWPGLNNSAD
                     QIWLKDLSGKIIDSLYYGAGWPIKAGYSAEKILPHLASDTSQSWALSVSPEHQTAGYF
                     NSVTRPENDLSLDSLQLSAITGDTNTVFAGCYFISNKGQNQACGANLIISLENDFTSQ
                     IPLDCIGPGQADSGNFEIGPLTSGKHTVTARVNWPADQINDNNMLLRSIQISWSRGSV
                     LLSEFMPYPWDIRIASSSIAEYLEFYNSGENTIDLAGWMISDQNTAVRRSIPSGYFLS
                     PGEYFVIAGDSSVLNFPDLAPSQTWITDKFLSLSNSEDAVYLYDPTGRSIDSLFYNST
                     WPIVQGIAMERISFDNSNRAMNWRASVAAAGGTPGQPNSVILPEKLDKLGIKPTADVF
                     SPDGDGIDDEIGIQYLLPFPSAKVTIEIYDLVGRLIYRLATNLETAASGVIYWDGASD
                     YSPKARVGIYIVRCLANDIASAKSVEYITTLVLAR"
     assembly_gap    8333..8432
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8433..10319)
                     /locus_tag="PLI55_05840"
     CDS             complement(<8433..10319)
                     /locus_tag="PLI55_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPB00390.1"
                     /translation="MNLAKATAAVLFIIGVFITGLDGQALTINEVMADPVGSDYYDEF
                     IELYNYGDLAVTLSGYYLEINGYVDTLSFVADYGDVLLPHSFALIMDRGYLVSNKSSS
                     YEDLIPENTLLVTIQDSSLARTGLINTGASQIYLFNAQGDTISAVLTTAKPLSGYSWE
                     KIIPEGTNQISNWGNSRNQRGTPGFWNSIAPRNYDVAIEGLKVVSPVDQIFPHQPIDV
                     QFTVKNFGLKAATSVQIICGLDQNSDRQIDEIFYDEMIALAAGDSAMKNATIPGLSSG
                     IYTILAEAIYAADERYDNNLDSLDIKILFSENCLIINEFMYYPATDGGEWVELLNISA
                     DTINLKKWMLSDNSTTIMLTGQDFYLPPQKYLVIVNDSTTFISYWGQVPFILDCPKTM
                     PALNNTKDSIIIRDHSAHLIDGLEYSSAWGYRQGVSLERKNSFASSNQASNWSLSTDL
                     DKATPGRRNSIDLRDYDLALDSVKVKPGKSPFQHNSPVTAQIYLRNNGKKPADSFKLN
                     IAIINSFLGTVSDTTITIFSPLQSNESQTVAVTFNAIPGGLHQISAEILWSADETLTN
                     NIANCPLAVGYPTGVLVINEIMFVPLAGQCEWFEIYNRSEITLDLNGWSFRDAMGKRL
                     ALADS"
     gene            10486..12201
                     /locus_tag="PLI55_05845"
     CDS             10486..12201
                     /locus_tag="PLI55_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00391.1"
                     /translation="MKKLIFGKFAFLKKTERFLLMLGLFGTLLSINLNAQSDRYTRKS
                     VAFLDALMVTDSRIRLNPEDEKYLLAAIHNRIRIARFDYNPLPDIVRYTFKEQLRARG
                     AVSESELVDLIEATIVPEIVKILDIEKEIRAQNLVDETQRNSFISLKAKELGITADQI
                     EQVMNASYIYLPFLTSYTVSKPKDDPNLTVTIKGGLFWYHIVPGDDPYLESIVTLHTE
                     ASGSAEKAKSDAESEAFRLAASTFAINLQVLTRDIEMFKLSAPIAAIEKRTVKFPLGT
                     AEGIKLDDPFFVGEYIQNSGDKITFKESGFVRVSKVGDNRQDPRQLSAAYAIQKGDWV
                     KGMTMVEHPRLGVDVALKPRGFFMNINEGVFESEDFLIFFDDYDGFAFGADLDFQINI
                     APLTNKRQSFLVAGATAGVVPVKSELYSTDTFIDWIIPDANNSASLVYGYTGYMRRFY
                     FGPVAIHGEALIGIQHLLLTDKYNEKDVTISNNSLGGRLNLALEYALNIDSNIGIFAG
                     FQAFPSFDWWTIKYGDEEIDVANYADTDKPSISSLSPTFGIYFHYSPPTLSFNPASAF
                     QANGN"
     gene            12219..13496
                     /locus_tag="PLI55_05850"
     CDS             12219..13496
                     /locus_tag="PLI55_05850"
                     /inference="COORDINATES: protein motif:HMM:NF040342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HPB00392.1"
                     /translation="MKKLSVTILTIFCIASLVFAKSEKSKTKSNLPRSHEAVILESTS
                     PSEVMVRATGYSSEGGKAAEMDKEAEADARKAAVWLILLGGTDPLLQTDAEKAEFAKI
                     QEEFFAPSNIKQFIVWESDYYHSRLKIDGGKTLKIEKTFKVNKSLIHDYLVERGILTK
                     TAAITEALGLPTIMVIPETDGKQAPIELLRTNPNLKKGAEVIEAYLTARKFEVIVPEQ
                     QQALQELTSAHYTLQGASEDYSYLLALSIGSDIYITYNVAVESRLLGSSTVKKGVVGC
                     RAYETTTGRLLGTETGYSPERTAPDAVLIEEAMNDAVDKVISRIMAYWKEDIQQGVQY
                     KVILNISDSFTKSEAEDMIYAFGDLLKKASKRIKENVMADYSYDVLIWCDPALYQSSS
                     DLYRFFKQNYKGQGRLERVSLNRKLILLNIVEE"
     gene            13506..14876
                     /locus_tag="PLI55_05855"
     CDS             13506..14876
                     /locus_tag="PLI55_05855"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HPB00393.1"
                     /translation="MQKQSLTKIILVLAGLAISSASAQVPDWYKTNQHKKYPASRYFL
                     GVAVADTKAEAIELARADVAKQIQVRIESELETIESEFREGDKNYLKAETTNRTKSIV
                     AETVSGIEIAEVKEVKGKTYVLAVLNKEKYLSTLEIQMDEILMKSEQLIASARQMVAD
                     GRILNALNNFIDAQNTIPDFYTKSSLYTALSGRKYPNFNQFTGPGIVAEVREILAHIE
                     LKILSGNNQIAVAGQNLPTPIKVQVVYVRDGEIIGVAGFPVIARYDGGEIITKIETDA
                     NGIAEFNCVATPTDNVGQSGAVKIALDLSKIPEQYRDAMTRSAVQINYILTANDLKFT
                     VEVIQKNGQHLTFIEDEVVRLIAANGYTCSADAPWLVQATLVIISQKEVPSPAGNQHL
                     VEAQLQLRLIEKQTGNVFAALDINTKGLDSQSPDSALSKAAQNLKIPSNKFAAFLQSA
                     NQPKND"
     gene            14904..15458
                     /locus_tag="PLI55_05860"
     CDS             14904..15458
                     /locus_tag="PLI55_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HPB00394.1"
                     /translation="MKKLLVFVLLISVALFTVNCGGKQPKAKMDDIPEWYLNPPQAED
                     AIYQAGDAVKSSMALAKDAADARARDAIARTVELRVTNMMKDFMQQSGMGEDAEALEF
                     TSSVSKQVANTVLKGCRIVKREMKVEGNNYHAYSLAEYNLNSLIEETLNEARRQKALY
                     NEAKANMSFEELEREIEKLKNVGQ"
     gene            15509..16252
                     /locus_tag="PLI55_05865"
     CDS             15509..16252
                     /locus_tag="PLI55_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00395.1"
                     /translation="MKFCLFTIALVLGLKNLAFAQECVPSWFEKLPEVEGVRLAVGYS
                     GKFTNEAGAKSAAVQLAHKNMTKQISIRLQFDIEELADGRIRLLNPSFKEFYEGSILN
                     AVESNSSVIDSTITVDGYFVLLAFPAIEYLPIPNACDKPWNTRPDWIENLPQEDGYVY
                     GIGIVARYRNWVRAWQDADEYARFDLGKNLQVNTESIHAVQRNNRTTIESVILKQTYD
                     LTINDATIVARWLDTANGVYYSLCRAPRP"
BASE COUNT         4663 a         3681 c         3438 g         4376 t
ORIGIN      
        1 ctgagaaaat gtagattgag ccggtagcac gaccagatgt aaataggttt actataatag
       61 cttggcaatt ataaaaccga cggcgaatcc ggtcaggaaa accattgtca caaagcgata
      121 gttgatgacc tcctttttat agcctctctt gattgccacc aaggaaacaa aataacctaa
      181 cgcattaaaa atccaaaaaa tcggcagggt taatacttta gccacaatcg gtccaattac
      241 aggcacagtt gccagcagag caattaagcc ggaaaaagcc tcggcaaata atccgaaaat
      301 cataaccagc acaactataa tacctttatc aacccgaaag tggattccta caataacggc
      361 aatgatgatt atcgcccagg taatcaataa cttgtaattc cgctggagaa agccgatgac
      421 attttgtatc aagcgcctca aaaacaaaag ggttcttttg tcattttgtt gctcgtcttg
      481 tttcatattt ttaccataaa attgtttata aagcctaagg ttacctcgac tgggatttcg
      541 acgtctgagg taggttcatc ccgtaaatca ggcgcaagcc atccagtgtt aaatagggat
      601 cgctggcttg gattaaggga gattgttgag caataacatt gctgaatcca ccggtggcga
      661 ttacaaaaat gtctggctga ttaatctcta ctccgatacg acgtatcatt ccttcaatca
      721 tagctagatg accaaacaaa atcccactct gcatatgagc ggttgtattc ttaccaataa
      781 cgcgcggtgg aaaagtgaga tttattggat agagctgggc ggcttttcta aaaaggtccc
      841 tcgcaccggt tataagtccg ggagcaatcg ctccgccgat ataatttcct tccttatcca
      901 gcacatcgaa agtcgttgcc gtgcccagat ccacgacgat gctcggacaa tgatatttca
      961 ctccagtggc tacggcattg caaatgcgat cagccccaac ttcagccgga ttatcaacta
     1021 aaagacaaca tccgagattc aaattgcaat tgattaggag cggttcaata tggaaaaagg
     1081 tttgcgccat ttgcttaaac ggttcggtta ggcgcggtac gactgagcca attacaatcc
     1141 catcaattac attaatggca atgccttgct ttttcaacaa agattcaatc cgccgaccat
     1201 aataatctgc tcctcgaacc ggatgactat ttattcgcag acatccctta aggtcatttt
     1261 cctgaaatag tcccacggtt gcattggtat tacctatatc aattgctaat aacattccta
     1321 agtctccctg acgattgtta cctcagcggc atggaacgtc cgatatgtcc gaccgacttg
     1381 tagaatgaga ccaccgtcgg cactgatatc atagaacaaa ccactgtgaa tcttttggcc
     1441 atcattgact ttaattttct gcccgataaa aggacaatat ttacgccatt tttccagaat
     1501 cggaacagtt ccgttaccat tcagcgcgac caaattttga tcaatcgcaa aaatcaattt
     1561 ggcgagcaca tcctctcttt catatttttt tccggtaata gaataaagcg aaccggcaaa
     1621 cggcaaatcg gggataaaaa aatcactatt ttgattcaaa tttacgccaa ttccaattac
     1681 cacatccgca ttatcccctg agctggtttt actttgcacc agaataccac agaccttcaa
     1741 atttttgagt aaaacatcat tgggccattt cagtgccaac caggctgggg taactagagt
     1801 ttgtagtcct tccagaacac ccaccgcagt tagaaatgag taaataaaaa gcggtgtctc
     1861 attgtggtga atttttaaat aaatagaaaa tagtaaagcc tcgttgggtg gagtaatcca
     1921 gctacgtccc aaacgccctc gcccagcact ttgaaaatca gcccagacta tcgtcccaga
     1981 aggatgcgaa tgccgaccca gtaggtaagc attggtagag tctatctctg caaaatgaac
     2041 caccggaaat ttgagtattt cagtaggaat cggcaggttg acgagccgga aagaattcat
     2101 aactttagcg caattcaagc tcttcaatca actcatcggc ggaactatat ttatccaaaa
     2161 taaaaagaac atagagaacg tcaacgctaa tagcacgcga aagagctgga ttatattgcc
     2221 aatcattggt cagcgcttcc cagttgccat cgaaagcaca gccgactaat tctccccgcg
     2281 cgtttagaac cggactgcca gagttgccgc cggtgatatc gcaggagctc agaaaagcca
     2341 ccggaacatc atttaactcc cgatcaatcc agcggccata atcgcggcta gtcgctagct
     2401 cgatcaatct ttctggaacg ttaaaaggtt caataccgct atgtttggca attactccgg
     2461 ctaaagtggt ctggggttgg taaaagacgg cgtcgcgcgg ctgatagcct tcgacgttgc
     2521 cataagtaaa ccgcaaactg cggttggcgt cgggcaaaac cgtcttaccc aaaacatttt
     2581 tcaaggccga gaaatattgc ggcttaagaa ttaacatttt gccgcttaat gttttctggt
     2641 ctttctcgat gcgctccagt tcgccgttga ctgcgacggc taaagttatc aatggatcct
     2701 gccgatcgag taaagactcg gcgctcaaat caaacattgc cagacattcg tcggcatcgg
     2761 taagtcgggt atgagcaaag gcactatcaa cataagcggc gataacggct ttagcagaat
     2821 cggcaggctg atcctgaatc aattcgcgca aaaaagcagg gcgctcagcc gaagaaaatt
     2881 cggctaaatg atttagtaat aatttcaaga tacgggcatc agccggggca tagtagccga
     2941 tgcgcctgac cttaaaatca cgcttggtcc gagcaatttg gtaatcaaaa aaaccgtatt
     3001 cacgttcact ttccggttta gccttttcac gactccattt ttcgatagtt aacgcatccg
     3061 ccagaaccgt aacgtaacgt aaatattcaa tgtacatatc gcgctcgaat ccggtataat
     3121 attcatcgta ggctttctgg atttctggca gaatattccc atattgttct tgccaagcag
     3181 gcttgccggc tattttttga actaattcct tctcaactaa ctttttttgt cccaaaatat
     3241 tttcactctt gaaaccagct aataaaccct gattattttt cagcccattg taaaaacctt
     3301 ttagtcgtga agataggagt aagtcaacct ccggactttt ttggctttca tcttcaagtt
     3361 cgttgatgat ttcttgaaag atccgaatgc gtagcggata tactaaattc tgattataag
     3421 ccacggagta actcgtgaca ttgcgatagg tgcgacccgg atagcccaaa atgaaaacaa
     3481 gctcatcggg attaagtcct ctggtagaaa tcggtagaaa agctttaggc tggtagggta
     3541 catttttctc gctatattcg gccggcttgc cgtcgggcgc gcagtaaatt cgcataaagg
     3601 aaaagtcacc agtgtggcgt ggccacatcc agttatcaac ctcgccacca aaattaccga
     3661 tagattgcgg aggggcataa accaggcgaa tgtcccgaaa ttttcggtaa tggtaaagat
     3721 aataactggc accatcgagc atagcgacga cctttcctgt cagtccattg gcagaatctt
     3781 cgctctcggc ttctaattgg gcgatagcgc gttccaaagc gcgagtgcgt tcttccggcg
     3841 tcatttgcgg ggtaaaggat ttaagtattt tttcagtgac atcttcaaaa ccaatcatta
     3901 gcgacgcctc atagccgggc gccggcagtt cctcggcttt agtgcgggca tagaaacctt
     3961 gtgccaagta attctgctca tcggttgaat ttttctgaat ggcactgaaa gcaacatgat
     4021 gattagtaag cactaaacca tctgccgaca caaatgaacc ggttccgcca ccgaataata
     4081 gcaccgctcc ggctaaaccg ccaccgtccg gttgatatat gtcggcgggt ttaactttca
     4141 agcctttgat tttggtcggg atttgatccg gcggatacat accttccgcg gctaatagtc
     4201 tgaaattcaa tgtcaaaaga caagtcgcca gaaaaaagag tcgttttatg ttactcatag
     4261 tttctttctc tctttttaat tgttaaaagc cacgttcttt gcgaatcgtt tgataagcgt
     4321 cgttgatgga tttgaatttt tcttccgcca gtttttgaaa ttcagggcca agattagcta
     4381 ctttatccgg gtgatatttc accgccaaat cgcggtaagc tttcttaatt atctcaacgt
     4441 cgtcatccgg tttaattccc aaaatctcat aagatcggtt ggcgctacca ctgaaaatcg
     4501 cccgaatagt ttcatagtca cgggcggaaa ttcctaaagc atccgaaatt tcccgaatcg
     4561 cttgcgattc tgccgtatgg agatcaccgt ccacagcagc aattccaaat aagacatgaa
     4621 gcagttccat ccgggagtag taatccatat tatcgcgaat ctgatgggca atgtcttgga
     4681 tattataatc tttaccaagt atctcccgat acaggtagag taaatcctga gcattccgag
     4741 ttccaaattg atcaactaga aatttcttta cgtattgaat ttcagaggat ttgacattcc
     4801 ggtcggattt actgatatag gtaaataacg agagcattgc caccgcaaaa tctccggcgc
     4861 gagtatcgcc gtaagtcgca aaggttgcac ccggctcggc tttacctcgt tttttctgga
     4921 ctccagattc gttggtaaca acattaccga tatacgcccc gattaaggct cccagtggtc
     4981 cgaaaagcgc ccagccgatt ccaccaccaa ttattgttcc agctaatttt cccatattct
     5041 tgatctcaat ttaaaatatt taactttaga aataataaat attccttgga taaaatccaa
     5101 cattaaataa aaatccctcc acctatgtaa gagtggaggg atcataacta tttggtataa
     5161 atactatctt aaagcaaaac cgacacccag accggcggca ttgtattctc ccatactata
     5221 ttcggcattg atagatataa ccgctatttt gatattcaag ccggcggtaa aacgcgtatc
     5281 attcttgcct tttaaatcga atttaatctt cgtgccgtct tccattttat aagaggcagt
     5341 gaatttactg gactctttgc caaccccaac atacggcgtt agcataagaa tagtcttact
     5401 gaccattaaa ttgaaagccg tgctattggc gctcaatagg tcaccgattt ccatttgttg
     5461 ataataacct tgaacggcaa tatctaatgg gaacatcggt atcggaatcc agcgatcaac
     5521 atccaatttt aaaccaccgc caagagccga gatttcgcca atagcttcac cgatatcgcc
     5581 aggaatttta aatttcggga ttattcggat agacggttcg atgcccatcg gcaatccaac
     5641 gctagcttgc aagatcggga atggaaatgg tacagtcttt aagtcttcca gagctttgat
     5701 tgatttcatc ccaggaatag atttcacttg aatattattt atggcatcct ctatttcagg
     5761 attcatctgt gcaatcatag cgtttatctg agcgtcggtc aaaagaggat attgggctct
     5821 taattgagtt gcaagctgag attcgatttc tccctggacg taaggttggt taaccctgaa
     5881 aggctccgct tccccgccaa ggatattcgg tgtagtacag tcagaaaaaa tctcttcagg
     5941 attcaaagta attgaatagc tctgggaatt cttctcaata ttgaacgcga tatcgtccaa
     6001 tgccgaaaga tcaaattgat aagtagtctg actggctttg ctggcgggtg ctaaacccac
     6061 tccagtcttt aattgaatat caaatcccaa agtgccgtgc gtcccagcag tgttatacag
     6121 accggagttc atcgccgtcc caaacccggt ggctaacggt tgcagatata acttcccgtt
     6181 agtagcggcc agttctttaa tctgatccat tgagaattcc tgtgccataa tcgtggctac
     6241 cgcaaaaagg gtcaatacaa caatccggct aagtgttctt ctcatatttt cctccatgat
     6301 ttcatttttg ctttaatgtt agggtcattt ttagtaaaaa tcaagtagaa ttttagcgag
     6361 ccaatactaa agtcgtgata tactccacag atttggcact ggcaatatca ttcgctaagc
     6421 atcgaacgat ataaattcca acgcgcgctt ttgggctata atcactagcc ccatcccagt
     6481 aaattacgcc gctcgccgct gtttcgaggt tcgtagccaa tcggtaaatc aggcgtccca
     6541 ccagatcata tatctctatg gtaacttttg ctgaaggaaa aggtagtaaa tattggattc
     6601 cgatttcatc atcaatccca tcaccgtctg gcgaaaatac atctgccgta ggtttaatgc
     6661 caagtttatc aagcttttca ggcagaatca cactgttagg ttgcccaggt gtaccgccgg
     6721 cggcggcgac acttgcgcgc caattcatcg cccgattaga gttatcaaat gaaatacgct
     6781 ccattgctat cccttggaca attggccagg ttgaattata aaatagactg tcaattgacc
     6841 gaccggtcgg atcatataag taaacggcat cttcactatt actcaaggat agaaatttat
     6901 cagttatcca ggtctgactt ggtgctaaat ccggaaagtt cagcaccgat gaatctccag
     6961 caattacaaa atattcacca ggcgacagaa aataaccgga cggaatgctc cggcgtacgg
     7021 cggtattctg gtcggaaatc atccaaccag caagatcaat ggtattttcg ccggaattat
     7081 agaattcaag atattcggca atcgaactcg aagctatccg aatatcccaa ggatatggca
     7141 taaattcaga tagaagtacg cttcctcttg accaggaaat ctgaatcgaa cgcaatagca
     7201 tattattatc gttaatctgg tctgcaggcc aattaacgcg ggctgtgaca gtgtgtttgc
     7261 cggaggttag cggaccaatt tcgaaatttc cactgtcagc ttgcccggga ccaatacagt
     7321 ccagtggaat ctgagaagtg aaatcattct ctaaagatat tattaaattt gcaccacagg
     7381 cttggttctg ccctttattg gatatgaaat agcacccggc gaaaacagta ttcgtgtcgc
     7441 cggtaatcgc ggacagctgt aatgaatcga gacttaaatc attttccggt cgggtaacgc
     7501 tattgaaata tccggcggtc tgatgttcgg gactgaccga aagcgcccac gattgggagg
     7561 tgtcactcgc caggtgtggc aggatttttt cagccgaata accggctttt attggccagc
     7621 cggctccata atagagcgaa tcaataattt ttccactcag atccttcaac caaatttgat
     7681 cagcggaatt attcagaccc ggccatttcc ccgggataat gaccgcagca ttgtccgtct
     7741 taaaataatt gaaacaggcg ctgtcgccgc ttaagattaa atatccacca gccggaactt
     7801 ttccacccga aatttcagct tttaaatagc gattacccag catccagccg gataggtcaa
     7861 tcgtttcatc gctgacatta atgcactcaa tgaactcaac ctgattgctg gtcgggtaag
     7921 ctaaaaattc gttgagggtt aaacagccgg ctggaaaagc taccactaaa tcggtaccag
     7981 cataattatc atcgagattc tcatctaagc tatattcaag ttctgccaaa taaagacttc
     8041 gcccggctct ctcggcagtt atcttccccg taacgatcag ggtcgagtcc gacttaaggc
     8101 tctggtttat atcctgtcgc caacaaagtt catctgcttg tctgcaacta tcctgatttt
     8161 gatccaaata gattttgatc gaaaaattct cagctggatt gcgcccgaca ttcttgatct
     8221 tcaatgaaaa actcaccttc tgaccgaccg catccggagt tttagaaaat ttaaatgacg
     8281 atggcacaat ggctaagtca ttatccagtt tgataatgga attagtctgg ccnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgaatcag ccaaagccag gcgtttaccc
     8461 attgcatcgc gaaagctcca gccattcaga tcaagtgtta tttctgaacg gttatagatt
     8521 tcgaaccact cgcattgacc agcaagtggg acaaacataa tttcattaat gactaaaacg
     8581 ccggtcggat agccaaccgc cagtgggcaa ttggcaatat tattagtcaa cgtctcatct
     8641 gccgaccaca gaatttcggc tgaaatttgg tgcaagcctc ccggaatagc attgaaagta
     8701 accgcgactg tttggctttc attcgactgc aagggagaaa aaatcgtgat agttgtatcg
     8761 gaaactgtcc ccaagaacga attaattatg gcgatattta acttaaatga gtcggcaggt
     8821 tttttaccgt tattgcgcag gtaaatttga gcggtgactg gtgaattgtg ttgaaacggc
     8881 gatttgccag gttttacttt aaccgaatca agtgcgagat cataatcccg caagtctatt
     8941 gaatttctcc gaccgggagt agctttatcc aggtctgttg agagcgacca gttactagct
     9001 tgattgctac tcgcaaatga attttttctc tcaagtgata cgccctgacg ataaccccag
     9061 gcactcgaat attctaatcc atcaattaga tgagcgctgt ggtctcggat tataatagaa
     9121 tctttggtgt tattcagcgc cggcatagtc ttaggacaat ccaggataaa tggcacttgt
     9181 ccccaataac tgatgaaagt ggttgagtca ttaacaatca cgaggtattt ttgtggcggc
     9241 agataaaaat cttggccagt tagcattatg gtcgtactat tgtcgctcag catccatttt
     9301 ttcagattga tcgtatcggc ggaaatattc aaaagctcca cccactcgcc gccgtcagtt
     9361 gccggataat acataaattc attgattatc agacaatttt ctgaaaacaa aattttaata
     9421 tccaatgagt caagattatt atcataccgc tcatccgccg cataaatagc ttctgccaaa
     9481 atagtgtaaa ttcccgacga caaacccggt atcgtcgcgt ttttcattgc ggaatctcca
     9541 gctgcaagcg ctatcatctc atcgtaaaat atttcatcta tttgccggtc ggaattttga
     9601 tccagtccac agatgatctg aacagaggta gcggctttca agccgaaatt tttgacagtg
     9661 aactgcacat caatcggctg atggggaaaa atttgatcaa ctggagaaac tactttcaat
     9721 ccttcaatgg ctacatcata attgcggggc gcaatgctgt tccagaaacc cggtgtgccg
     9781 cgctggttgc gactattgcc ccagttagaa atttgattag taccctctgg aatgattttt
     9841 tcccagctgt agccggacag gggtttggca gtcgttagta cggctgatat tgtatctccc
     9901 tgagcgttaa aaaggtatat ttgactggca cccgtattta tcaggccggt gcgtgccaag
     9961 gaactatcct ggattgtcac cagcagggtg ttttcaggaa tcaggtcttc gtaagagctc
    10021 gatttattac taactaaata gccgcgatcc ataatgagag cgaaactatg cggcaaaaga
    10081 acatcaccat aatcggcgac aaaactcaac gtatctacat agccgtttat ttccaggtag
    10141 taaccgctaa gggtaacagc cagatcacca taattgtaca actcaataaa ttcatcatag
    10201 tagtctgacc cgactggatc agccatcact tcgttaattg taagggcttg accatccaaa
    10261 ccggtgataa acacgccgat tatgaaaagc accgcggctg ttgcctttgc taaattcaaa
    10321 aactctccaa cttcttgccg ttattttatg tcttaaatca cagaaagtca atgataaatt
    10381 cattaagtat ttcctcgctt gtaaaaaaag ttgattttca gatttatttt taagaaaatt
    10441 aagagtgttc gatttttaac aaatagaaaa tttatggagg gaagtgtgaa aaaattaatt
    10501 tttggcaaat tcgcattttt aaagaaaacg gagcgcttct tattgatgct tgggctgttc
    10561 gggacattat tgtcaattaa tcttaatgcc caaagtgatc gttatacccg caaatcggtg
    10621 gcgtttctgg atgcattgat ggttactgat tccagaattc gcctcaatcc ggaggacgaa
    10681 aaatacttgt tggccgcgat tcataacagg atccggattg cccgctttga ttataaccct
    10741 ttgccggata ttgtgcggta cactttcaaa gagcaattgc gggccagagg tgccgtctcc
    10801 gaatcggaac ttgttgacct aatcgaggct accatagttc cggaaattgt aaaaatactc
    10861 gatatagaga aggaaatccg cgcccaaaat ttggtggacg agacccagcg caattcgttt
    10921 atttcgctca aagccaaaga attgggtatt accgcggacc aaatcgaaca agtaatgaac
    10981 gcttcgtaca tctaccttcc gtttcttacg agctatacag tcagcaaacc caaagatgac
    11041 ccaaacctga cggtaacaat caaaggtggt ctgttctggt atcatattgt tccgggcgat
    11101 gatccgtatt tggagagcat cgtgactcta cacaccgaag cctccggttc cgcggaaaaa
    11161 gctaaaagcg atgctgaatc cgaagctttt cggcttgccg ccagcacttt tgccattaac
    11221 ctgcaggttt tgacccgcga tattgaaatg tttaagctga gcgctccgat agccgctatc
    11281 gaaaagcgca ccgtgaaatt tccgctcggc acagcggaag gcattaaact agacgaccct
    11341 ttctttgtcg gtgaatatat ccagaacagc ggcgataaaa tcacttttaa ggaatcaggg
    11401 tttgtgcgcg tcagcaaagt tggcgataat cgtcaagatc cgcgacaact ttctgcggct
    11461 tatgctattc aaaagggaga ttgggtaaaa ggaatgacaa tggttgaaca tccccggtta
    11521 ggtgttgacg tggctttaaa gccgcgcggg ttttttatga atatcaacga aggtgttttt
    11581 gaaagcgagg attttttaat cttttttgat gattatgacg gtttcgcctt cggcgccgac
    11641 ctggattttc agatcaatat tgctccatta actaacaaac gtcaatcatt tctggttgca
    11701 ggcgccactg ccggagtagt gccagttaaa agtgaattat atagcactga tacttttatt
    11761 gattggataa tcccggacgc caataattcg gctagcttgg tttacggcta caccggttat
    11821 atgcgacggt tttattttgg gccggtggca attcacggcg aagccttaat tggcattcaa
    11881 catttacttt taaccgataa gtacaatgaa aaagacgtta cgatttcaaa taatagtctc
    11941 ggggggcgcc tgaatctggc actcgaatat gcccttaata ttgatagcaa tatcggcatt
    12001 tttgccggtt ttcaggcttt tccgtcattt gactggtgga cgattaaata cggagacgaa
    12061 gaaattgatg tcgctaacta tgctgatact gacaagccct ctatttccag cttgtcacca
    12121 actttcggca tttatttcca ttattcgccg ccgactttat catttaaccc agcatcagcg
    12181 ttccaagcca atggtaacta aactaaacga ggagtaaaat gaaaaaatta tcggtaacaa
    12241 tattaactat tttttgtatt gctagtttag ttttcgccaa atctgagaaa tcaaagacga
    12301 aatcaaatct tccgcgttcg cacgaggctg tcattttaga aagcacttca ccttctgagg
    12361 taatggtgcg tgccaccggt tacagttccg agggcggcaa agccgccgaa atggataaag
    12421 aagccgaagc cgatgctcgt aaagccgccg tctggctgat cttattagga ggtaccgacc
    12481 cacttctgca gaccgatgcc gaaaaagctg aattcgctaa aatccaggaa gaatttttcg
    12541 cgccctccaa cataaaacaa ttcatcgttt gggaatccga ttattaccat tctcgcttga
    12601 aaattgacgg cggtaaaacc ttgaagatcg aaaaaacttt caaagtcaac aaaagcctaa
    12661 tccacgatta tttggtagaa agaggtattc ttaccaagac tgccgctatt actgaagcgc
    12721 tcggcttgcc gacgataatg gttatcccgg agacggacgg taaacaagca ccaatcgaac
    12781 ttttgagaac caatccgaac ctcaaaaaag gtgcggaagt aatcgaagcc tacttgacgg
    12841 ctcgcaaatt cgaggtaatt gtacccgaac aacagcaggc gctccaggaa ttaacttccg
    12901 cacattacac cctccaagga gccagcgagg attacagtta cctgctggca ctctcaattg
    12961 gtagtgatat ttatattacc tataatgtcg ccgtcgaatc tcgcctatta ggttcatcaa
    13021 cagtaaaaaa gggtgtggtt ggttgtcgtg cctatgaaac tacgactggt cgcttgctcg
    13081 gcaccgaaac tggctatagt ccggaaagaa ccgcgccgga tgccgttttg attgaagaag
    13141 ctatgaatga tgccgtagat aaagtcatca gccggattat ggcttattgg aaagaggata
    13201 ttcagcaagg agtgcaatat aaagtgattt tgaacatcag tgattccttc accaaatctg
    13261 aagcagaaga tatgatttac gcttttggtg atctgttgaa aaaggctagc aaaaggatta
    13321 aggaaaatgt aatggctgat tattcctatg acgttttgat atggtgtgat cctgctcttt
    13381 accaatcttc atccgatttg tatcgttttt tcaaacaaaa ttataaaggc cagggtcgcc
    13441 ttgagcgggt cagtttgaac cgcaaactaa tcttgttaaa catcgtcgaa gaataagagg
    13501 tcattgtgca aaagcaatca ttgactaaaa taatattagt tttagcaggt ttggcgattt
    13561 catcggcatc ggctcaggtt ccggattggt acaaaaccaa ccagcataaa aaatatcccg
    13621 ccagccgcta tttcctgggc gttgccgtgg cagatactaa agccgaagcc atcgaactag
    13681 cgcgggctga cgtcgctaag cagattcagg tgcgtatcga gtctgaactc gaaaccatcg
    13741 agagcgaatt tagagaaggc gataaaaatt acttaaaagc cgaaactacc aatcgcacta
    13801 aatccattgt cgcggaaacc gtctcgggaa tcgaaatcgc cgaggtcaaa gaggtcaagg
    13861 gcaaaaccta cgtcctcgcc gtgctcaata aggaaaagta tctctccact ttagaaatcc
    13921 agatggatga gatcctgatg aaaagcgagc aacttatcgc gagcgcacgc cagatggtcg
    13981 ccgacggtcg aattctaaac gcgctgaata atttcattga tgcccagaat acaatcccgg
    14041 atttctatac aaaaagttca ttgtacaccg ccctttccgg tcgcaagtat cccaatttca
    14101 accagttcac cggaccggga atagttgcgg aagtgcgtga aattttagcg catatcgaat
    14161 taaaaattct gtcgggaaat aatcaaattg ccgtcgccgg tcagaatttg cccacaccca
    14221 ttaaagtgca ggttgtctat gttcgcgacg gcgaaatcat cggagtcgcc ggttttcctg
    14281 ttatagctag atatgatggc ggtgaaatta ttactaaaat cgaaactgac gcgaacggaa
    14341 ttgctgaatt taattgcgta gctactccaa ccgacaatgt cggccaaagc ggggcggtta
    14401 aaatcgccct agatttatcg aaaatcccgg aacagtatcg cgatgcgatg actcgctccg
    14461 cagttcagat caactacatc ctgactgcta atgatctaaa atttacggtc gaggttattc
    14521 agaaaaacgg ccaacacctg acattcatcg aagacgaggt agtgcgattg atcgccgcca
    14581 atggctatac ttgttcagcg gatgcgccct ggctagttca agcaactttg gttattatct
    14641 ctcagaagga ggttccctcg ccggctggca atcagcactt ggtagaagct cagttacaat
    14701 tacgcttgat agaaaagcaa actggcaacg tttttgccgc actggatatt aatactaaag
    14761 gactcgattc tcagtcgccc gatagtgcgc tctccaaagc cgctcaaaac ctaaaaattc
    14821 caagtaataa atttgccgct tttttacaaa gcgccaatca gcccaaaaat gattaattcc
    14881 aaaaagtaac aaaaggagaa agtatgaaga agttactcgt atttgttttg ttaatttccg
    14941 tagccttgtt cacggtcaat tgtggcggta aacagcctaa agccaaaatg gatgatatcc
    15001 ctgaatggta tctcaatccg cctcaagctg aagatgccat ctatcaagcc ggcgatgcgg
    15061 ttaagtccag catggccctc gccaaagacg cggctgatgc tcgcgctcgc gacgctattg
    15121 cccggacagt agaactcaga gtcaccaaca tgatgaaaga ctttatgcaa caaagtggta
    15181 tgggagaaga tgccgaagcc ctcgaattca cttcttccgt tagcaaacag gtcgccaaca
    15241 ccgtcctgaa aggttgccgg attgtcaagc gggaaatgaa ggtcgaaggc aataactacc
    15301 acgcttattc tctcgccgag tataacctga actcattgat cgaggaaact cttaacgaag
    15361 cccggcggca aaaagctctt tataatgaag ccaaagccaa tatgagcttc gaagaactgg
    15421 aaagagaaat cgaaaaactt aaaaacgtcg gtcaatgatt gaaccgttaa agcccgtttg
    15481 gctctaacca gacgggcttt ttttctctat gaaattttgc ctttttacta ttgccctggt
    15541 cctcgggcta aaaaatctgg cgtttgctca agaatgtgtc ccttcttggt ttgagaaatt
    15601 gcccgaagta gaaggagtcc gtctggcagt cggatattcg ggtaaattca ccaacgaagc
    15661 cggcgctaaa tccgccgccg tccaattggc tcacaaaaat atgactaagc aaatttccat
    15721 tcgactgcaa tttgacatcg aggaactggc tgatggacga atacgcctgt tgaatccatc
    15781 ttttaaagaa ttctatgaag gttcaatttt aaatgcagtt gaaagtaact cttccgttat
    15841 tgactcaaca ataaccgtgg acggttattt cgttttattg gcatttccag caatcgaata
    15901 cctgcccatt cccaatgctt gtgacaagcc ctggaacaca cgaccggact ggattgaaaa
    15961 tcttccccaa gaagatggct atgtctatgg aattggtatt gtcgcccgat accgcaattg
    16021 ggtgcgcgcc tggcaagacg ccgatgaata cgcccgcttc gatctcggca agaatttgca
    16081 ggttaatact gaatcaatcc acgccgtcca acgcaacaat cgcaccacga ttgaatcggt
    16141 gattttgaaa cagacctatg atctgacaat caatgatgct acgatcgtcg ctcgctggtt
    16201 agatactgct aacggtgtct attattcgct ctgccgagca cccagacctt gaggggca
//
LOCUS       DAOOMU010000046        16030 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_3847_length_16030_cov_16.218216, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000046 DAOOMU010000000
VERSION     DAOOMU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16030
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3847_length_16030_cov_16.218216"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /gene="hypD"
                     /locus_tag="PLI55_05870"
     CDS             complement(<1..1025)
                     /gene="hypD"
                     /locus_tag="PLI55_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPB00396.1"
                     /translation="MIKYIDEYRNPELITKAVERIRAITPNKIVNLMEVCGGHTITIF
                     KFGLKKLLPENIHLISGPGCPVCVTDSEFIDRAIEIAKFSDVIITTFGDMVRVPGSYG
                     SLQSARAGGADVRTCYSPLEAVEIAERNPAKEVVFLGIGFETTAPNIASAILNANSRH
                     ISNFSVMSGHKTMPKAMQSLLDSGEIRLDGFICPGHVTAITGPEIYQFIADEYKIPCV
                     VAGFEPLDMLEAIFRLIKQIVERRAEVENQYRRAVKPGGNRKAQRIMAEVFEPCDTIW
                     RGMGNIPRSGLQIRSKYRQFDADYKFQVKLPPTKSVSGCICGDIMRGVKIPPECRLFR
                     KICTPDNP"
     gene            complement(1022..1258)
                     /locus_tag="PLI55_05875"
     CDS             complement(1022..1258)
                     /locus_tag="PLI55_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPB00397.1"
                     /translation="MCLAVPMRIIEIDGSRAKAEVGGVLYQANLDFLSDVKVGDYIIV
                     HAGFAIEKLDEIVALDNLAAWQEVATDNKTKYRK"
     gene            complement(1258..3594)
                     /gene="hypF"
                     /locus_tag="PLI55_05880"
     CDS             complement(1258..3594)
                     /gene="hypF"
                     /locus_tag="PLI55_05880"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HPB00398.1"
                     /translation="MISHKIIITGIVQGVGFRPFIYNLARQYQLTGWVRNNSHGVEIV
                     AQGEPIQLACFAEIIQNSPPPRGRIERFEVTQFITAEKFLEFRIVESQNEQMKSVQIS
                     PDLDVCLDCRREMFDSSNRRYLYPFINCTNCGPRFTIIQDVPYDRQLTTMHEFKMCPE
                     CQCEYDDPSNRRFHAQPNACPVCGPRLQLLDNASRELLTGGSADANRAVFEKVAELLR
                     DGRIIAVKGIGGFHLACDARNEAAVTSLRQRKFREDKPFAVMFPSLPDIAKYCKYSRE
                     EARLLNSVPHPIVLLQKLPGRDVASAVAPGNHYLGCFLPYSPLHYLLMYFFPYPLVLT
                     SGNVSDEPIAYRDDDALARLNRIADYFLIHNRKIHIRCDDSVYRIWNNKTYPLRRSRG
                     FAPSALPYKNGFVRPILACGPEQKNTFALAQSNHIYLSQHIGDLVNFEVLQSLKNGIS
                     HFKNIFAIEPEIVAYDLHPDYLSTKFALEYPDETPDGQKVIKIGVQHHHAHAVACLAE
                     NNGAEPAIGIILDGTGYGTDGIIWGGEILKVEIHRFERLGHLRPARLPGGSAAIKYPW
                     QMAVSYLYETLGSEITIPKFPFLENVPPQQIGIVNDMLKSSFNSPLTTSCGRLFDGVA
                     ALAGLRNQVNYEGQAAVEFEQCIASENYRSAYDFEIETGTDSFVIDWRVMIEQVIEDV
                     KHQTGIELIAVKFHNGLANILLQAVLKSQEMTGLNTVALSGGVFMNIYLLTRLSQLLN
                     EHNFKVCTHHLVPCNDGGIALGQAVIANAIIQNQLEKS"
     gene            complement(3587..4288)
                     /locus_tag="PLI55_05885"
     CDS             complement(3587..4288)
                     /locus_tag="PLI55_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPB00399.1"
                     /translation="MCGRKTLTKGKMEIIAELSIKEWDSSFDIQPNYNIAPTSIMPIL
                     VYENGRIVRAMRWGLIPEWAKDATSVPIMINARCESLTVKPSFRGLLDSQRCIVITDG
                     YYEWQKTESRKQPFYIHHPEKRLLTMAGLYSRWVASDSQSYLTYTVITTPANAQLQFI
                     HQRMPAILTPEEIDTWINCRQYDRQKALTLLQPSRMPLIAYPVATFVNSVKNNSPQCI
                     QPINLTAEQTNLLND"
     gene            complement(4505..>5686)
                     /gene="gdhA"
                     /locus_tag="PLI55_05890"
     CDS             complement(4505..>5686)
                     /gene="gdhA"
                     /locus_tag="PLI55_05890"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPB00400.1"
                     /translation="ERVIIFRVPWVDDRGEVQVNVGYRVQFNSAIGPYKGGLRFHPAV
                     TLSMMKFLAFEQTFKNALTTLPMGGGKGGSDFNPTGKSENEIMRFCQSFMTELHRHIG
                     PYLDVPAGDVGVGGREIGYLFGQYKRIVNRFEAATLTGKGLAFGGSMLRPEATGYGAV
                     YFVVEMLKTKGETIKGKIVGVSGFGNVAWGVAKKATELGAKVISLSGPDGCIIDPDGV
                     GTEEKWNYMLEMRASGRDVVKDYADKFGVKFYPKARPWDIVPMDIAMPCAFQNEVSLE
                     NVQNLIKNGCQILAEVSNMGCTPEAFKLANAKMMYAPGKAVNAGGVAVSGLEMCQNSE
                     RLSWSAEEVDEKLKWIMTNIHSQCLAAAAEVGAPGNYAKGANIAGFRRVAEAMLEQGI
                     V"
     assembly_gap    5687..5786
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6374..9076
                     /locus_tag="PLI55_05895"
     CDS             6374..9076
                     /locus_tag="PLI55_05895"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HPB00401.1"
                     /translation="MEKWWQLNISQLCEKLSSDPEKGLSPEEASKRLTQYGPNQLREQ
                     KSRHPIFLFLDQFRDFIIWVLIGAAILSGFLGEWIDALAILAIVIFNAILGFVQEYRA
                     EKAIVALKKLSTPMAKVIRDGNQMTIPSNEVVPGDIIEIEAGDHIPADSRVLWHTSNF
                     SVEEASLTGESLPVQKNAESIEKPEIPLGERTNMVFAGTSVVSGKARAMVVSTGMQTE
                     LGKIADMILTIEHETTPLQKRLEAFGHWIVYLCFAIVAIVFVMELLRGGKLIDIFLTA
                     VSLAVAAIPEGLPAVVTIALALGVQRMVRRHVLIRKLPSVETLGSATVICSDKTGTLT
                     KNEMTVQSLYVGHRVYRVTGVGYNPQGEFFEDDKPLNREEIPLELEKALRCAVLCNAA
                     RLFKTEQGYGISGDPTEAALLVAGAKLGIWKEAEEKKYPFVDELPFDSNRKLMSMIRK
                     NGEQWVAFVKGAPDILLQRCDFIEINGSIRPLTEEDRIQIQEANQRFGNEALRVLGLA
                     YRTLDRIEKPFSTEQVENHLVFLGLIAMMDPPREEVKPAILACKTAGIQPVMITGDHK
                     TTAVAVAKALQIFSPETKALTGEELDQIDEATFQQEVKDISVYARVSPEHKLRIVRAW
                     KSRGDVVAMTGDGVNDAPAVKEADIGVAMGITGTDVTKEVSDMVVLDDNFASIVAAVE
                     EGRGIYDNIKKFIYYLLSCNAGEILMMFFSSLLGLPIPLFPIQILWVNLITDGFPALA
                     LGVEPLDPHVMERPPRPKDEGIVTKKTIFILLLQGFFIAFTSLLAFILVLKVENEGLA
                     RARTAAFIVLAVSQLFHSFNYRHFTESVFKIGFLGNKKLVLATGVSFLLQMAVVYIPF
                     LRTLFKTMPLLWWDWLLVIGLSSCTLWFMELVKWIWRKKGFSPF"
     gene            complement(9290..9733)
                     /locus_tag="PLI55_05900"
     CDS             complement(9290..9733)
                     /locus_tag="PLI55_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPB00402.1"
                     /translation="MKKLYRSRKHRIIGGVCGGIAEYFEIDPVLVRLIWVILVIFGGM
                     GILAYIIAWIIIPNRDESNSTVEQPDQPSEPQSSTEVATNTSNKNLFWGIVLIVIGLL
                     IIANQFWWPFDFIRTLVRGIFKYFIPAILIVLGIFIILQRKETKK"
     gene            complement(9753..10487)
                     /locus_tag="PLI55_05905"
     CDS             complement(9753..10487)
                     /locus_tag="PLI55_05905"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HPB00403.1"
                     /translation="MQQIINHPWNVSIKEARAIQLQLAKEVILQQFTGNIAKIWAVDV
                     SYGRFDSVGYGVLGWFVTADDSSGGQFDCQMIKTFTTISSVAFPYVPGYLSFREIPVL
                     LPLFQELPEKPDLVLVDGAGIAHPRGIGLAAHLGVIFNVPTIGCAKSRLFGNYTEPAN
                     ESGAFSQLTNDDNIIGFVLRTKKNTKPLFISPGHLTDPQSSLEIIKRFCGKYRIPEPL
                     RRVDFISKELRRNPDQFQSNIIYCNQ"
     gene            complement(10488..11231)
                     /locus_tag="PLI55_05910"
     CDS             complement(10488..11231)
                     /locus_tag="PLI55_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPB00404.1"
                     /translation="MKPPVILIIKTGSTYSDLRNAFGDFDIWIKNAAGDFNCNWKVKP
                     VADVEPDKIPNYQGVIITGSHSSLTQFYAFYGGMERVVQQIIDHRIHTLAICFGHQLV
                     HKIMGGTIDANPLGTELGVVTINLTLQGLVDPLFAGQSGSRIQVYASHSDIVIKPAPS
                     FVQLAWNKLALFQATRYDDFIYTTQFHPEYTKEIMDWYLKKNLALLTKQHISNPLHFA
                     TADEILKRNKSLPKSKLIIQNFLNLINSK"
     gene            complement(11237..12577)
                     /gene="trkA"
                     /locus_tag="PLI55_05915"
     CDS             complement(11237..12577)
                     /gene="trkA"
                     /locus_tag="PLI55_05915"
                     /inference="COORDINATES: protein motif:HMM:NF007038.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPB00405.1"
                     /translation="MKIIIIGAGEVGYYLAKRLISEDHNVTIIEMDSERHRRAAETLD
                     AIVIHDSGSSPRVLENAGVKSADILLAVTGSDDTNILSCIIAKKLGVARCLARVHSEE
                     FSFSKSAITGQDIGIDLLIHPERTAAEATIRLVEQSSASQLVTFEDDRLQIISLLVKE
                     DSPVVNLTLKEVATQHDQFSFLCLAIHREGKTIIPRGKNVYRSGDTAYFIAKEEDIPR
                     LTALLGYPKTEQQHIMILGAGRLGRMVAAVLSREMNVKLIEQDRDIAEEVAVNLPDTL
                     VLHGDGTDIDFLSSERIEEMDCFVAVSGSEKTNLLSGLLAKHLGVKRVIIHVTTHEYI
                     PLMDKIGIDAVVSKNVETVNAIMRYVRRGNVIAVSLFEDIKAEAIELIPHVGSDITKK
                     PIKDLELPEDMIIGALIRQKEIIIPRGDTQIQPQDKVVVFLKPNLIQKVEKYFN"
     gene            complement(12581..13621)
                     /gene="ltaE"
                     /locus_tag="PLI55_05920"
     CDS             complement(12581..13621)
                     /gene="ltaE"
                     /locus_tag="PLI55_05920"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HPB00406.1"
                     /translation="MSKIRIDLRSDTVTKPSPEMRAAMASAEVGDDVYGEDPTVNRLQ
                     ERVAALLGKEAALFVPSGTMSNQIAIKCLTQPGDEIICEYESHIYTYEASGPAFNSLV
                     MPRPVKGHYGVMAIEDVVRAIRPDNIQTGQTSLIEVENTHNRAGGTIYLLAEIEKLAE
                     IANEHRIPMHLDGARLMNAAVSTGIAPAEYAKYFDSVSLCLSKGLGAPIGSVLSGSRN
                     FIAKALRVRKIFGGAMRQVGILAAAGLYALDHNVQRLKIDHENAWLLAQGLSEIEGLY
                     IDKQQVQTNMVMIFARSPRWTTEKIVQQLLEKGIGMNAVDQERIRAVTHLDVSRDQIL
                     ETIEIFKKIMEA"
     gene            13769..15235
                     /locus_tag="PLI55_05925"
     CDS             13769..15235
                     /locus_tag="PLI55_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPB00407.1"
                     /translation="MNPEREKSLILLFENWADEKAQNIYQLPSSGSYREYYRIQGNKQ
                     TALGVFNRDHKETRAFIEFARHFRSIGLNVPRIYADDAKNNIYLIEDLGDLTLYSYLV
                     NNYKNGIFPDKLIDMYKKVIAYLPRFQILADKGLNYSFCYPRSKFDKRSMLWDLNYFK
                     YYFLKLAKVSFNEEELENDFQTFCDFLLQADCNYFLYRDFQSRNILLVDSEPYFIDFQ
                     GGRKGALQYDIASLLYDAKADIPQHLRDEFLDEYIRQVSRYLTIDEKSFREFYWGYVL
                     IRIMQAMGSYGFRGFYEKKEHFLKSIPYAVANLRFILNNVTLPIEIPALWQALTQIVE
                     SSKYSNNSAVTSVTSSVLKVRINSFSYRNGIPADPSGNGGGFVFDCRGIPNPGRYEKY
                     KSLNGHHPEVIKFLKEETDIDEFLDYVYAIVDKTISNYIERQFTNLMVSFGCTGGQHR
                     SVYCAEELARHLRDKFRIQIELNHTALENTSGLSEIHS"
     gene            15330..>16030
                     /locus_tag="PLI55_05930"
     CDS             15330..>16030
                     /locus_tag="PLI55_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPB00408.1"
                     /translation="MKALILAAGLGKRLRPLTNQKPKALIEVGGRPLLQIAIEYLQMY
                     GFDEIIINVHHHADQIIKYLEVNKYLGQNIIISDETDQLFDTGGALKKAAWFFEDSQP
                     FLVYNVDILCNLDLRQFYQHHRESGALVTLAVRQRSSERYLLFDEANVLCGWQNLKTS
                     EIIQTRPSDSPLCQLAFSGISVLSPEILRHLPHKNIFSLIDLYLRLAPEFIIKGFQHN
                     ESRWLDVGNPENLAKA"
BASE COUNT         4569 a         3565 c         3235 g         4561 t
ORIGIN      
        1 ggattatccg gcgtacagat tttcctaaat aaccgacatt ccggtggaat tttgacgcca
       61 cgcataatat ctccacaaat acaacctgaa acacttttag tgggaggcaa ttttacctga
      121 aatttataat cggcatcaaa ttgacggtat ttagatcgta tttgaaggcc actcctagga
      181 atattgccca tcccgcgcca gattgtatcg cagggttcaa aaacttctgc cattattctc
      241 tgggctttgc ggttaccgcc tggtttaacg gcgcggcgat actgattttc gacttccgcc
      301 cgccgttcga caatttgttt aattaaccta aaaatcgcct caagcatatc gagcggctca
      361 aatccagcta caacacatgg gattttatat tcgtcagcta tgaattggta aatttcgggg
      421 ccagttatag cagtaacatg tcccgggcaa ataaaaccat ccaaccttat ttcgccggaa
      481 tccagcagag attgcattgc tttcggcata gttttatgac ctgacatcac cgaaaaattt
      541 gagatatgcc gactattagc atttaaaatc gccgaggcaa tattcggtgc agtcgtctca
      601 aaaccgattc caaggaaaac gacctctttc gccggattcc gctcagcgat ttcaaccgcc
      661 tcgagcggcg aatagcaggt ccgcacatcg gcacccccag ctctggctga ctgcaaacta
      721 ccgtaacttc ctggaactcg caccatatca ccgaaagttg taattatgac gtccgagaat
      781 ttagcaatct cgatcgcccg atcaataaat tcgctatcgg tcacacagac cggacagcct
      841 ggcccgctaa tcagatggat attctccggt agcaattttt tcaatccgaa tttaaaaatg
      901 gtaattgtat gaccgccaca gacttccatt agattaacaa ttttattcgg tgtaattgct
      961 cgaatgcgct cgacggcttt ggtaattaat tccggattgc ggtattcgtc aatgtattta
     1021 atcattttcg gtatttggtc ttgttgtcgg tagcaacttc ctgccaggcg gcgagattat
     1081 cgagcgccac gatttcatcc aatttctcaa tggcaaaacc ggcgtgtacg atgatataat
     1141 caccgacttt aacgtcactc aaaaagtcga ggttggcttg gtataggact cctccgactt
     1201 cagctttggc gcggcttcca tcaatttcaa taattctcat cggcacagcc agacacatta
     1261 tgacttctcc aattggtttt gaataattgc gttggcaata accgcttgcc cgagagcaat
     1321 tccaccgtcg ttgcaaggta caagatgatg agtgcagact ttgaaattgt gctcgttaag
     1381 caattgcgat agacgcgtca gcaagtaaat attcataaaa acgcctccgc ttagcgccac
     1441 cgtattaagg ccggtcattt cttgggattt caaaacggct tgcagaagaa tatttgccaa
     1501 tccgttatga aatttgacag cgattagctc gattccagtt tgatgtttga catcctcaat
     1561 gacttgttca atcataacgc gccaatcaat gacaaagcta tcagttccgg tttcgatttc
     1621 aaagtcataa gccgagcgat aattttcaga tgcgatgcat tgttcaaatt ccaccgccgc
     1681 ttggccttca taatttacct gattacgcaa acctgccagc gccgctactc cgtcgaaaag
     1741 gcgaccacag gaagtcgtta ggggcgagtt gaaactagat ttcagcatgt cattcacgat
     1801 cccgatttgt tgtggcggaa cattttccag aaatggaaat tttggtatag taatttccga
     1861 acccaaagtt tcgtagagat agctaactgc catttgccag ggatatttaa ttgcggctga
     1921 accacccggc agacgagctg gtcgcaagtg tccaagtcgt tcaaagcgat gtatttccac
     1981 tttcaggatt tcaccgcccc agattatgcc gtcggtgccg tagccggttc cgtccagaat
     2041 aatgccgatt gccggttcag cgccattatt ttcagctaaa caagcgacag cgtgagcgtg
     2101 atgatgttgg acaccaattt taatgacctt ctggccatcc ggagtttcat ctggatattc
     2161 gagggcgaat ttagttgaaa ggtaatccgg atgtaagtca taggctacga tttccggttc
     2221 aatagcgaaa atatttttaa aatggctaat cccattttta agtgactgta acacctcaaa
     2281 attcactaaa tcgccgatat gctgacttaa gtatatatga ttgctttgcg caagggcaaa
     2341 agtgttcttc tgttccggac cacaagccaa aatcggacgg acgaatccat ttttataggg
     2401 cagagcgctc ggcgcaaaac cacgcgaacg tcgcagagga taagttttat tattccatat
     2461 acgataaact gaatcatcac aacggatatg aattttgcga ttatgtatta aaaaataatc
     2521 ggcaatccgg ttcaatcggg caagtgcatc gtcatctcga taagcaattg gttcgtcgct
     2581 gacattgcca ctggtaagca ccaacgggta cgggaaaaaa tacataagta aatagtgcaa
     2641 tggcgaatac ggtagaaaac agccgagata atgattgcca ggtgccacag ccgaagcaac
     2701 atccctgcca ggcaattttt gcagtaaaac aatcgggtga ggcacactat tcaatagccg
     2761 cgcttcctcc ctactgtatt tacaatattt agcaatatcc ggaagacttg gaaacataac
     2821 ggcaaacggt ttatcctccc ggaattttcg ttgacgtaga gacgtgacag ccgcttcatt
     2881 gcgggcatcg caggctaagt gaaaaccgcc gatacctttg accgcgataa tccgaccatc
     2941 gcgcaacagt tcagcaactt tttcaaaaac agcacggtta gcatctgcac tgccgccggt
     3001 aagtaattcg cggctggcat tatcaagcaa ttgcagacgc ggcccgcaga ctggacaggc
     3061 atttggctga gcatgaaaac gccgattgga cggatcgtca tattcacact gacattccgg
     3121 acacattttg aattcgtgca tcgtcgtcag ttggcgatcg tatggcacat cctgaataat
     3181 cgtaaaacgc ggcccacaat tcgtgcaatt aataaaggga taaagataac gccggttgga
     3241 cgaatcgaac atttcacggc ggcaatccag acaaacatcc agatcgggag aaatctggac
     3301 acttttcatt tgctcatttt ggctttcaac aatcctaaat tctaaaaatt tctcagccgt
     3361 tatgaattgc gttacctcga agcgctcgat acgaccgcgc ggcggcggag aattttgaat
     3421 tatctctgca aaacaagcta gttgaattgg ttcgccctga gcaacaattt ccacgccgtg
     3481 cgaattattt cgcacccaac cggtaagctg atattgcctg gcgagattat aaataaatgg
     3541 tcggaaaccg actccttgta caattccagt aatgattatt ttatgactaa tcatttaaca
     3601 gattggtctg ctcggcagtt agattaatcg gctgaataca ttggggagaa ttatttttca
     3661 cggaattgac gaaagtcgca accggataag cgatcagcgg cattcgcgac ggctggagaa
     3721 gcgtcagcgc tttttgccga tcatattggc gacaattaat ccaggtgtca atttcttccg
     3781 gcgtcagaat cgcaggcatt cgttggtgaa taaattgcaa ttgggcatta gccggagtgg
     3841 tgatgacggt ataagtaagg tatgactggc tatccgacgc aacccaacga ctataaagcc
     3901 cagccatcgt cagcaatctc ttttcgggat gatgaatgta gaacggttgt ttacgacttt
     3961 cagttttctg ccattcataa tagccgtcgg taattactat acagcgctga gaatcaagca
     4021 atcccctaaa agaaggctta acggttagcg attcacagcg ggcattgatc ataattggca
     4081 ctgaggtagc atcttttgcc cattcgggaa ttagacccca gcgcatcgcc cgcacgattc
     4141 gtccattctc atatactaaa atcggcataa tgctagttgg tgcaatgtta taattcggct
     4201 gaatgtcgaa tgaactatcc cactctttga tggaaagctc cgcgatgatt tccatcttgc
     4261 ctttagtcag tgtctttcgt ccgcacataa aatcctgcct tttaccttga tcaattcctg
     4321 actaatacca tcttttaata taaactgggt actcggtaaa aaccaacatt tcttgagagg
     4381 taaaatcaaa gatctccttt cccactattg accatttcag gtcgggaaat gacaaaagat
     4441 aaaagatttt ttatagtgca gaaccccgtg cttattaaaa tagaatacgc gattttaatt
     4501 caaactaaac aataccttgc tcaagcatag cttcggcgac tcttctgaag ccggcaatgt
     4561 tagcgccttt ggcgtaatta ccgggagctc cgacttccgc ggctgcggca agacattgcg
     4621 aatgaatatt tgtcataatc catttcagct tctcgtcaac ttcttccgca ctccaagaca
     4681 aacgttccga attctggcac atttccagac cggaaaccgc cactccaccg gcattgacag
     4741 cttttccggg agcatacatc attttcgcat tcgctaattt gaaggcttcc ggagtacaac
     4801 ccatattcga aacttcagct agaatctggc atccattctt gatcaaattc tgaacatttt
     4861 caaggctaac ttcattttga aaagcacaag gcattgcaat gtccatcgga acaatgtccc
     4921 agggacgggc tttcggatag aatttaacgc cgaatttatc cgcataatct ttaacaacat
     4981 ctcggccgct ggcgcgcatt tcaagcatat aattccattt ttcctcagtt ccaactccgt
     5041 cggggtcaat gatacagccg tccggaccag ataatgaaat tactttcgct cccaattcag
     5101 tagctttttt agcaactccc caagccacat tcccaaaacc tgaaacaccc acgatttttc
     5161 ccttaattgt ttcgcctttg gttttcagca tttcgacaac gaaatacacg gcaccatagc
     5221 cggtagcttc cggccggagc atactaccac caaaagccaa acccttgccg gtcagagtag
     5281 ccgcctcaaa gcgattcact atccgcttat attgtccaaa aagataacca atttcgcgac
     5341 caccaactcc tacatcaccg gcagggacat caaggtacgg accaatatga cgatgcaatt
     5401 ccgtcataaa gctctgacaa aacctcataa tttcgttctc ggatttaccg gtcggattga
     5461 aatctgagcc acccttacca ccacccattg gcaaagtcgt taaagcattt ttaaaagttt
     5521 gctcaaaggc taaaaatttc atcatactta atgttactgc cggatgaaaa cgtaagccac
     5581 ctttataggg accgatcgcg ctgttgaatt gaactcgata accaacgttg acttgcacct
     5641 caccacggtc atcaacccag ggaacccgga aaatgattac tcgttcnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnaaat catacgagta aaaattaata aagattttaa
     5821 taaaacttat aaaaatttat agtaattgac agagatttgg aataacctcg taactctttg
     5881 atataaatga tagtttgcca ataaggcatc aaaatagacc gaagaattta gagtaatgta
     5941 gaaattatct tatccaaaaa atctttattc cgttgaggtt aaacaaacta aaattttgag
     6001 ggagtcttga taaaaatgga ttgatttcac cgtagcctgt cattaaattg aattgttttt
     6061 tatgagaaat agtgatataa aaaaaatata cagcgaaata ggagctattt ttacaatttt
     6121 tctcttattt tgttttatac aaaacaaagt ccattatctt tatttagaaa agcgccctgc
     6181 cctaagagca tatcttttaa aaacctgtta cccctggatt ggggagtgcg aaaataggga
     6241 actttcggac ttaatagtag attgtaaaat caacattttt cgaaataaaa cctcattata
     6301 tatcacaaaa cccgatctct tttaaacaac acgcaatcgc ttatgaataa aattctaaca
     6361 agggaatagc attatggaaa aatggtggca attgaacatt tctcaattgt gtgaaaaact
     6421 ttcttcggat cctgaaaagg ggctttcgcc agaagaagcc tcaaaacgtc ttactcaata
     6481 tggtcctaat caactccgag aacagaagag cagacacccg atttttctat ttttggatca
     6541 atttcgagat tttatcatct gggtattgat tggggcggct attttatcgg gttttctcgg
     6601 ggaatggatt gatgccctag ccattctcgc catcgtcatt ttcaatgcca ttttggggtt
     6661 tgtccaggaa taccgggcgg aaaaagcaat tgtggcatta aagaaattgt ccactcctat
     6721 ggctaaggtg attcgagatg gaaatcaaat gactattcct tcaaacgaag tagttcccgg
     6781 cgatataatc gaaatcgaag caggagatca cattccggct gattcccgag tgctctggca
     6841 tacttcgaat ttcagtgtcg aagaagctag tttgaccggg gaatcgctcc ccgttcaaaa
     6901 aaatgctgaa tccattgaaa aacctgaaat tccattggga gaacgtacga atatggtttt
     6961 tgcggggact tcagtcgtca gtggaaaagc cagagctatg gtagttagta ccgggatgca
     7021 gacagagttg ggcaaaatcg cggatatgat tctcaccatt gagcacgaaa ccacgcctct
     7081 gcaaaaacga ctcgaagcat ttggtcattg gattgtttat ttgtgctttg ccattgtagc
     7141 gatagtattc gttatggaat tgctccgcgg gggcaaactt attgatatat tccttacagc
     7201 cgtgagtctt gcggtggcgg caattccaga aggattacca gccgtggtaa ctatcgcatt
     7261 ggctttaggg gttcagcgta tggtgcgacg ccatgttctc attcggaagc tcccttctgt
     7321 ggaaactttg ggctctgcca ccgtaatttg ttcagataaa accggtactt tgaccaaaaa
     7381 tgaaatgacc gttcagtccc tgtatgtggg acatcgagta tatcgggtaa ctggagtagg
     7441 atataaccct caaggagaat tttttgagga tgataaaccc ctaaatagag aagagattcc
     7501 attagaatta gaaaaagccc ttcggtgtgc cgtattatgc aatgccgccc gtctttttaa
     7561 aacggaacag ggctatggaa tttcaggtga ccccacggaa gccgcgttgt tagttgccgg
     7621 agccaaatta ggaatttgga aggaagcaga ggaaaaaaaa tatccatttg tagatgaact
     7681 gccttttgat tcgaatcgga agcttatgag tatgattcgg aaaaatggtg aacaatgggt
     7741 tgctttcgtg aaaggcgctc ccgatatatt gctccaacga tgtgatttta ttgaaatcaa
     7801 cggatctatc cgccctttga cagaagaaga tcgaatccaa attcaagaag caaatcagcg
     7861 atttggtaac gaagcgttga gagtattggg tttggcgtat cgaacgctgg atcgcatcga
     7921 gaagcctttt tccacagaac aagtagaaaa tcacttggtt tttctgggat taattgctat
     7981 gatggatcct ccccgagagg aagtaaagcc agccattttg gcatgtaaga ccgcaggcat
     8041 tcaaccggta atgatcactg gcgatcacaa aaccacggcc gtggcagtag ctaaagccct
     8101 tcaaattttt agccccgaaa ccaaagcctt gactggcgaa gaattggatc aaatagatga
     8161 agccactttc cagcaagaag ttaaggacat ttcggtttat gcccgcgttt ctcccgaaca
     8221 caaactccgc attgttcgag cttggaaatc ccgtggtgat gtagtcgcta tgactggaga
     8281 tggcgtaaat gatgctccgg cagtgaaaga agccgatatt ggagtggcta tgggaatcac
     8341 aggcacagat gtaaccaaag aagtgtcgga catggtcgta ctggatgata attttgcttc
     8401 tatcgttgcc gccgtagaag aaggcagagg aatatatgat aacattaaaa aattcattta
     8461 ttacctgctt tcctgtaatg ccggagaaat cctgatgatg tttttttctt ctctcttggg
     8521 cttgcctatt cctctattcc cgattcaaat tttgtgggtg aatttgatca cagatggatt
     8581 tcccgccctg gctctcgggg tcgagccgct ggatccccat gtaatggaaa gaccaccgcg
     8641 tcccaaagat gagggcattg ttactaagaa aaccattttt attctcctgc tccaaggatt
     8701 ttttatcgca ttcactagtt tactcgcctt tatccttgtg ttaaaagtag agaatgaagg
     8761 tctggctcgg gctcgaaccg cggcttttat tgtccttgcc gtgagccaac ttttccattc
     8821 gtttaattat cgccatttta ctgaatcggt gtttaaaatc ggatttttgg ggaataaaaa
     8881 actcgttctg gcgactggag tttcttttct ccttcaaatg gcagtagttt atattccttt
     8941 tctaagaact ctgttcaaaa ctatgccctt actgtggtgg gattggcttc ttgtgatagg
     9001 actttcttct tgtaccctct ggtttatgga acttgttaaa tggatttgga gaaaaaaggg
     9061 attctcacct ttttagaaat acacggattt tagatattaa attttggcaa tgcgatgctt
     9121 aaccgtgtca ttttcggctg gattgtcctt attaaatgtt tcttgatggt cttttattca
     9181 gatgagagaa tccccgacat taagacaaga gcaattcttc cagtatacaa agaataaacc
     9241 ccaaattgaa atagaaaaaa gctaatgaat ttttatgctt ttcaattagt tacttttttg
     9301 tttcttttct ctgcaaaatg atgaaaatac ctaacacaat taaaatagct ggaataaaat
     9361 acttaaaaat tcctctcacc agagtccgaa taaaatcaaa tggccaccag aattgattgg
     9421 caatgattag cagacctata acaatcagca cgattcccca gaaaaggttc ttattcgacg
     9481 tgttagttgc tacttcagtt gatgactgtg gttcggaagg ttgatccggc tgttcgactg
     9541 tcgagttcga ttcatctcga tttggaataa taatccaggc aatgatataa gccaatatgc
     9601 ccattccgcc aaaaataacc agaattaccc agattagtcg tactaaaacc gggtcaattt
     9661 caaaatactc agcaatgcca ccacaaactc cgccaataat tcggtgcttg cgactacgat
     9721 aaagtttttt cattttctac ctcacctcta atttattgat tacagtaaat tatattgctt
     9781 tgaaattgat cgggattccg acgtaattct tttgagatga aatctaccct tcgaagcggt
     9841 tcgggaatcc gatatttccc acaaaatcgt ttgataattt cgagcgatga ttggggatcg
     9901 gtcaggtgac cgggtgaaat aaaaagcggc ttggtattct ttttcgttcg caggacaaat
     9961 ccaataatgt tatcatcatt agtcaactga gaaaatgccc cactttcgtt agccggttcg
    10021 gtataattac caaatagccg cgacttggca caaccgattg taggcacgtt gaaaattact
    10081 cccagatggg cggctaatcc gattccgcgt gggtgagcaa ttccagcccc atctactaac
    10141 accaaatcag gtttttccgg taattcctga aatagaggta aaagcaccgg aatttcgcga
    10201 aaactcagat atccgggaac atacggaaaa gcgactgaac taatcgtcgt aaaagtcttg
    10261 atcatttggc aatcaaattg cccgccagaa gaatcatcag cagtaacaaa ccagcccaag
    10321 accccatatc cgacgctatc aaagcgtcca taagagacgt caactgccca tattttggct
    10381 atattaccgg tgaattgttg gagaatcacc tccttagcta gttgtaactg aatagctctg
    10441 gcttctttga ttgatacgtt ccagggatga tttataattt gttgcattta tttactgtta
    10501 atcaaattaa ggaaattctg gataatcagt ttggactttg gtaatgattt gttccgcttg
    10561 agaatttcat cagccgttgc gaagtggagg ggattagaaa tatgttgttt tgttaatagc
    10621 gccaggttct ttttcaaata ccaatccatt atttccttag tatattccgg atgaaattgg
    10681 gtagtataaa taaaatcgtc atagcgcgtc gcctgaaata gcgccaattt attccaggct
    10741 aattggacaa aactcggggc tggttttata acaatgtcag aatgggaagc gtaaacctga
    10801 atccgactac cgctctgccc ggcaaatagc ggatctacta agccttgtag agtaagattt
    10861 atggtaacga cacctaattc ggttcccaaa ggatttgcgt caatcgtacc gcccataatt
    10921 ttatgaacca gctggtggcc aaaacagatt gcaagtgtat gaatgcggtg gtcaattatt
    10981 tgttggacca cacgttccat tccaccataa aacgcgtaaa actgagtaag cgaagaatgg
    11041 gaaccggtta tgattacgcc ttgatagttt gggattttat ccggttcgac gtcggcgacc
    11101 ggcttcactt tccagttgca attaaaatct ccagcggcat ttttgatcca aatatcgaaa
    11161 tcaccaaacg catttcttaa atcgctataa gtcgaaccgg ttttaattat aaggataacg
    11221 ggcggtttca ttcgatttaa ttgaaatatt tttcgacttt ttgaatcaaa ttaggtttaa
    11281 gaaagaccac tactttatcc tgaggctgaa tctgggtatc gcctcttggt atgattattt
    11341 ccttctggcg aatcagcgct ccgattatca tatcttccgg caattcaagg tctttaattg
    11401 gttttttagt gatgtcgctt ccgacgtgtg gaattaattc tatggcttcg gctttgatat
    11461 cctcgaataa tgagacggcg atgacattac cccgccgtac ataacgcatt atcgcattaa
    11521 ctgtctcaac atttttactg acaactgcat cgatgcctat cttgtccatt agaggaatat
    11581 attcatgagt agtaacatga ataatgacgc gtttaactcc aagatgcttg gcaagcaaac
    11641 cggaaagcag attggtcttt tcactaccag aaactgctac aaaacaatcc atttcttcta
    11701 ttctttctga actaagaaaa tcaatgtcgg taccatcacc atgcaagact aatgtatcag
    11761 gtaaattaac ggctacttct tcagctatat cacgatcttg ctcaatcagc ttgacattca
    11821 tttcacgcga taaaacagct gcgaccattc tcccgagccg accagcgccc agaatcataa
    11881 tatgctgttg ttcggtcttg ggataaccga gcagtgccgt taaacgcgga atatcttctt
    11941 ctttagcaat aaagtaagcc gtatcaccac ttctgtaaac attttttccc cgcggtatga
    12001 tggtcttacc ttcccgatga atcgccaaac ataaaaatga aaactggtca tgctgagtag
    12061 caacttcttt caaagttaag ttaactaccg gactatcttc ttttactaac aatgaaataa
    12121 tctgcaaacg gtcatcttca aaagttacca attgagatgc cgaggactgc tccaccagcc
    12181 ggatagtagc ctcggccgcc gtccgttccg gatgaatcaa taagtcaata ccgatatcct
    12241 gaccggtaat agccgacttt gaaaaactga attcttcact atgaacacgc gctaaacaac
    12301 gggctactcc caactttttg gctattatac aggatagaat attggtgtcg tcacttcccg
    12361 taaccgctaa aagaatatcg gcagatttta ctccagcatt ctccaaaact cgcggactgg
    12421 aaccactatc gtgaataaca atggcatcaa gtgtttctgc ggctcgacga tgacgttccg
    12481 aatccatttc gattatagtt acgttatgat cttcgctaat aagacgcttg gctaaataat
    12541 aaccaacttc accggcgcca ataataatta ttttcattgc ttaagcttcc ataattttct
    12601 taaatatttc aattgtttca agtatttgat cacgactaac atcgagatga gttacagcgc
    12661 gaattctttc ctgatcaacg gcattcatcc caataccttt ttctaataat tgctgaacaa
    12721 ttttttcagt cgtccagcgc ggactacggg caaaaatcat caccatattg gtttgcacct
    12781 gttgtttatc gatatacagt ccctcgattt ctgacagtcc ttgtgccaat agccaggcat
    12841 tttcgtggtc aattttcagg cgctggacat tatggtcgag ggcatataaa ccagccgcgg
    12901 ccaaaatccc aacttgtcgc attgctccac caaaaatttt cctaactcgc agggctttag
    12961 caataaagtt ccgcgaacca cttaaaaccg atccgatcgg cgcgcccaga ccttttgaca
    13021 aacatagact caccgaatcg aaatatttag catattcggc gggggcaatg ccggtgctca
    13081 ccgccgcatt catcaggcgc gcaccgtcta agtgcatcgg aatacgatgt tcattggcaa
    13141 tttcagccag tttctcaatt tctgccaata gataaatcgt accaccagca cgattatgag
    13201 tattttcgac ctcgattaaa ctcgtctgcc cggtctgaat attgtctggt cgtatagctc
    13261 tgacaacatc ctcgattgcc ataactccat aatgtccctt caccggacgc ggcattacca
    13321 acgaattgaa tgctggaccg ctagcttcat aggtataaat gtgactttca tactcgcaga
    13381 taatttcatc gcccggttga gtaagacatt taatcgcaat ctggttcgac attgtcccac
    13441 tcgggacaaa taaagcggct tccttgccca gaagagccgc taccctctcc tgcagacgat
    13501 ttaccgtcgg gtcttcgcca taaacatcgt caccaacttc ggcgctcgcc atcgccgccc
    13561 gcatttcagg agaaggtttg gtaactgtat cgctccgtaa atctatgcga attttactca
    13621 taccgttaac atttccttta taaattttac cgtttcaaat taattaatta ttagcggttt
    13681 ttcaaattca ggatgatggt ttgggaaata attttcccaa tccagaaaaa tccttccata
    13741 atgactgcga acatccgaaa ttatagctat gaatcccgaa cgggaaaaat cattaatcct
    13801 gcttttcgag aactgggctg acgaaaaagc ccaaaacatt tatcaattac ccagttccgg
    13861 ttcataccgc gaatactatc ggatacaagg aaacaagcaa accgccttgg gagttttcaa
    13921 tcgcgatcat aaggaaactc gggcgttcat tgaatttgcg cgccatttcc gctcaattgg
    13981 attaaatgta ccgagaattt atgccgatga tgcgaaaaat aacatctacc ttatcgaaga
    14041 ccttggcgat ttgaccttat attcctacct tgttaataat tataaaaatg gtattttccc
    14101 cgataaactg attgatatgt ataaaaaagt catcgcgtat ttgccgcgtt ttcaaattct
    14161 ggctgacaag ggtttgaatt actccttctg ttatccgcgt tcgaaattcg ataagcgctc
    14221 aatgttatgg gatttgaatt atttcaaata ttattttctg aaactggcaa aggtcagctt
    14281 taatgaagag gaacttgaaa acgatttcca gaccttttgc gactttcttc tacaggctga
    14341 ttgcaattat ttcctctatc gcgattttca gtcgcgcaat atattgcttg ttgatagcga
    14401 accatatttc attgattttc aaggcggtag gaaaggcgcc ttacagtatg acattgcctc
    14461 tctactctat gatgcaaaag ccgatatccc ccaacatttg cgtgatgaat ttctggatga
    14521 atatattcgg caggtcagtc gctaccttac gattgacgaa aaatcgttcc gtgaattcta
    14581 ctgggggtat gttttgatca ggattatgca ggcgatgggt tcctacggat ttcgcggatt
    14641 ctacgagaaa aaagaacatt ttctgaaaag tatcccctat gccgtagcca atctgcgttt
    14701 tattttgaat aacgtgactc tgccaattga aatcccagcc ctttggcaag cactaactca
    14761 gattgtagaa tcatcgaaat attctaacaa ttcggcggta acttcagtca cttcaagcgt
    14821 cttaaaagtc cggataaata gcttttctta ccggaacggc atcccagccg atccgagcgg
    14881 aaacggtggt ggttttgtct tcgattgtcg tggcattcca aatcccggcc gctacgaaaa
    14941 atacaaatct ctcaatggtc atcatccaga agtcattaaa ttcttaaaag aagagaccga
    15001 cattgacgaa ttcctggatt acgtctatgc cattgttgat aaaactataa gtaattacat
    15061 cgaacggcag ttcactaatc tgatggtaag tttcggctgt actggcggtc agcatcgctc
    15121 ggtttattgt gccgaagaac tcgctcgtca tttacgtgat aaattccgaa tccagatcga
    15181 actaaatcac accgcgctgg aaaatacatc aggactttca gaaattcatt catgaacatt
    15241 ttaggtaaaa ctcctatttc tcattttatg gtttttagtt tcaagcctgc cctaaaatta
    15301 ccgtccgtta acaatttgcg atcaccaata tgaaagctct tatacttgcc gctggactcg
    15361 gtaaacggct ccgaccgctc accaaccaaa aacctaaagc attgatcgaa gttggtggtc
    15421 gtccccttct gcaaatcgca atcgaatatt tacaaatgta tggctttgat gaaattatta
    15481 tcaatgttca tcatcatgcc gatcaaatca taaaatatct tgaggtcaat aaatatctcg
    15541 ggcaaaatat tataatttca gatgaaactg atcaactttt cgacacggga ggcgctttaa
    15601 aaaaagccgc ctggtttttc gaggatagcc aaccttttct ggtttataat gttgatattt
    15661 tgtgcaatct cgacctgagg caattttatc agcatcaccg cgaatcgggc gcgttagtca
    15721 ctttggcggt tcgccagcgt tcgtcggaac gttatttact tttcgatgaa gctaatgtcc
    15781 tatgtggttg gcaaaatctc aaaaccagcg aaattatcca gacgcgtccc tccgattcac
    15841 ctctgtgtca attagctttc agcggaatct cggtgctcag tccggaaatt ctccgccatt
    15901 taccccacaa aaatattttt tcgctgatag acctctatct tcgtctggcg ccggaattca
    15961 tcattaaggg ttttcagcat aatgaatccc gctggctgga cgtcggtaat cccgaaaatc
    16021 tggcgaaagc
//
LOCUS       DAOOMU010000047        15969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_3865_length_15969_cov_18.897700, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000047 DAOOMU010000000
VERSION     DAOOMU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15969
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3865_length_15969_cov_18.897700"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLI55_05935"
     CDS             <1..198
                     /locus_tag="PLI55_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00409.1"
                     /translation="FSHIKAPLDIYRDRAKEIIVRHAVKKTDKTSNTKRIAILVVFRD
                     IFICSHRTPSNKSAATFIALF"
     gene            complement(401..1588)
                     /locus_tag="PLI55_05940"
     CDS             complement(401..1588)
                     /locus_tag="PLI55_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00410.1"
                     /translation="MNLPADRLIKKIFVTAECARDEIVEQIFTAYPKQKKIAIESEKS
                     LTETIPQEDPKKILLVTHSKGEVVKDCPGTAAPYLCCRYQVINQTLNCPLNCTYCILQ
                     YYLNQPATIIYTDYQKIIAEIEQKITSQPHRLLRFGTGELGDSLALTASQIFAIKLIG
                     YFATQKNALLELKTKTDRIGLILDQPHNGHTVISWSLNPLVIRNSEEHLAPPPTRRLK
                     AAAEAARRGFLIGFHFDPIIDIPDWESQYLDLIEELYHYVDPARIAWISLGSLRFPPS
                     MKTKIIARYPYSRLPYGELIRGQDQKMRYPRPRRTEIYRSIYRKLTEIREPPFVYFCM
                     ESPTVWQDVCGFAPQSNAHLDYLFAESLARRFPGLVAIKPKLPVYENAAGGCSAVKNN
                     PDY"
     gene            complement(1608..2558)
                     /locus_tag="PLI55_05945"
     CDS             complement(1608..2558)
                     /locus_tag="PLI55_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00411.1"
                     /translation="MQIKKIAIEQIIPSQSQERLFVEEINPDCNKPNESLFLQPVFLH
                     HARDGFTILYGFNYISNIYQCGGKNIQAFVIPKSQSLFSALNIVADFIRRSRPLWPIE
                     IARILQLFNQQQFSNAKKSAFFKKFTEKDLTRTLEERYLALIGLNPLLHKFLIEKKAP
                     LKTWFLTAECPPVIRDLILNLITECQPSLSTFEEISRNLLEILRREDIAPEQLTAEMS
                     QVLIEKAEEIQPSQKLEIFRSKIQERRFPQMTIHRERLNNFLQTIQLPSAVEIEFNPN
                     FEERGYRLKAEIRTPNDLRELTEFLNSAAQSDLKHLLEIL"
     gene            complement(2533..3396)
                     /locus_tag="PLI55_05950"
     CDS             complement(2533..3396)
                     /locus_tag="PLI55_05950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008337748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB00412.1"
                     /translation="MDDPQKISVIIPTYNRETCLERAIVSVLSQTYRNFELIVIDDGS
                     TDNTSRIIHKYKNKIRYFSQLHSGVSTARNLGLEKSEGTWVAFLDSDDYWLPRKLERQ
                     MQCLAENPQWLVMQTDEKWIRNGVVVNPTKKHQKYSGWIFVQCLPLCIVSPSAVIVHQ
                     KVFNDVGVFDESLPVCEDYDLWLRVSLKYEIGLLPEKLIIKTGGHSDQLSRKYWGIDR
                     YRVLALEKILNAELDRQQTELVLDEIIKKLTILSKGRTKHPELPNIYADKLSDYQKKK
                     EVFLANQKNCH"
     gene            complement(3389..4051)
                     /locus_tag="PLI55_05955"
     CDS             complement(3389..4051)
                     /locus_tag="PLI55_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00413.1"
                     /translation="MVLTGSKSRDVILIGAAFKAEINPLIEKLSQASFSPTGIDFDYI
                     LTGSGLERVRCCLAEKVVCQDYLAIINIGTAGVLNDTCPLMSIFYPHRFCTLSNDHLT
                     YLTLPNDFLESSHFLSTGWKSGTLFSSREPVTSAAKRWAILQNSQADVVDMEAFAYAD
                     FCQRHKIPFACLKVITDRADAEVKQNFNANLAESAQLLAQNALIFVDFLLNKLDIKTE
                     NG"
     gene            complement(4143..4424)
                     /locus_tag="PLI55_05960"
     CDS             complement(4143..4424)
                     /locus_tag="PLI55_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00414.1"
                     /translation="MSDNFNNSGKKGSAQPYDSKINEQCPKCSGNIVYRKSTYFGNKV
                     YYKVCNNCGWYITLPKEVWKDIISASLKPAKATPSESAEQDEQIEAIVS"
     gene            4719..5492
                     /locus_tag="PLI55_05965"
     CDS             4719..5492
                     /locus_tag="PLI55_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPB00415.1"
                     /translation="MNEKQSHGPTISQAQEDYLKIILELINSRRVARIKEIAQRKNVS
                     MPTVTEAMRKMAQDGLINYSAHEFIDLTSEGEQAAHQMTARHKFLRLFLEDILNIPSE
                     TADTEACLLEHHLSPITLERMILLYQFLVNCPHHDSNLIEDFRQCLSAEKLADKTSCR
                     DCFVKIAFPHTVTNPFAVHSLLANLSEGQKGTVTMLGGNTAMRHKLIEQGLVPGATVQ
                     MERTGNKSQPFVISVNGLQIKLDIEQANLIEVAISSGEI"
     gene            5495..5752
                     /locus_tag="PLI55_05970"
     CDS             5495..5752
                     /locus_tag="PLI55_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013867642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HPB00416.1"
                     /translation="MSTNSTTLVARSLAEVPAGRCFRIKKVHGHGEIRQRLIDLGFIK
                     NEVGTVIREALLKDPIEVELKGTHFSLRRSEARLIEVEVID"
     gene            5749..7941
                     /gene="feoB"
                     /locus_tag="PLI55_05975"
     CDS             5749..7941
                     /gene="feoB"
                     /locus_tag="PLI55_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPB00417.1"
                     /translation="MNFPPHRHRHRRGQRQISPDSSQKVIRIALAGNPNSGKTSLFNA
                     ITGQHQHIGNYPGVTVEKKYSLFHYKDDVIEIIDLPGTYSLTAYSLEEIVARDFVIKE
                     KPDVIIDVLDATNLERQLYLLLQFQELGVPLVGALNMSDECEKKGIDIDHERLSSILG
                     IPLVKTVGTRGYGIDCLLDTAIRVAKGKLRADQRHLNYGEDVENAHDQIIKVLETDPD
                     FSHKYPVHWLAIKLLENDRDAIQKVKSEHTNAETVLGESERIRRQLENHFHDDIISIL
                     AERRYAYINGAIREAVRIDRTRPAIDWTERIDRVILNRFLGIPIFLLIMFLIYQATFG
                     LGNPLAGLISNSFANLADFVNLHLPDNRFRDFLTNGLIAGVGGVLVFLPLVVLLMLGL
                     SLLEDTGYMARAAFVMDKFFHLFGLHGRSFIPLMLATGCAVPGVMAARSLANRRDRII
                     TIMVTPLMMCSAKTPVIAMLAAAFFPEKAGLIFWGIWFLSWFMALIIALIFRKTFFKG
                     DQSPFVMELPPYRWPSFRSVMIHMWQRTLAYLRKAGTIILFAAIIVWFALHNPTNPGS
                     HSTDASQFNVNNNQGSETVNQGQQLYHSYAGQIGRGLEPILKWAGFDWKIGVSLIAGL
                     AAKEVIISTLGMLYGIPEGAQEATEQSKMTTVSYQISRDPNYSPLSAFSLMIFVMLYT
                     PCLATLAVVRKELGHIKWALFQAVYTVTIAFIASVAIFQIGTFLQHIF"
     gene            8112..10727
                     /gene="mutS"
                     /locus_tag="PLI55_05980"
     CDS             8112..10727
                     /gene="mutS"
                     /locus_tag="PLI55_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPB00418.1"
                     /translation="MTKSTEKIDETTPVMRQYNAVKAQYPDALVLFRMGDFYETFRED
                     AKQAAKILNITLTKRANGKAAAVPLAGFPYHALESYLHKLLKAGLKVAICEQVEDPKL
                     AKGVVKRAVVEIITPGTAISDRFLEKEKNNYLVSVYINGENAGLAALDISTGEFYLSE
                     IPTARITEAVLDLHPAEIICPESIQKEIQTLFTNQVLRITPLEDWIFTFETAYQTLIE
                     HFKTDSLKGFGCQSAVHGITAAGPILHYLRQNYQTNLQHIYSIRNLATDETVLIDDFT
                     RRNLEIFQTMQSGDRKGTLMDVIDQTVSPMGVRLLTKWLIRPLRDPAAINQRLDGVEF
                     LYNNTKIRHQLREMLAHCGDLERLISRLSTNRASAREIVALKNTLKLIPGIRDLLPNQ
                     LAFSEINSLLNPLPELVELLERAIKDEDLPLSIHEGGFIKDGYSAELDEYRHIARHGK
                     EWIARLQETERQKLGIPSLKVGYNKVFGYYIEVTRTHAEKIPPEYIRKQTLVNNERFV
                     TPELKEYEEKLLNAEERINQIEFELFQDLRTKILQFIEAIQKNAGALAQIDVLAGLAE
                     LAEKNRYCRPVINDGTAIDIKAGRHPVVEHLLPPGEKFIANDLHIDNLSEQILIITGP
                     NMAGKSTYLRQIGLIVLMAQIGSFVPADSATIGVVDKIFTRVGASDNLAAGESTFLME
                     MHETANILNNVTPHSLVLLDEIGRGTSTYDGMAIAWSVTEYLHNQPNVAAKTLFATHY
                     HELTELEKILPRLKNYNVAVREYGDRVIFLRKIVPGGCDKSYGIHVAQMAGVPPEVIG
                     RAREILTNLSPEEKVLPTNREKFRHLPEKDANQLGLFGDDLKLKKMLAEVDIDNLTPL
                     EALQKLAEIKKSLGG"
     gene            10785..11894
                     /locus_tag="PLI55_05985"
     CDS             10785..11894
                     /locus_tag="PLI55_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00419.1"
                     /translation="MLRRIEFKIVLLGLLAWVLLLNPVNLLAIGDKYAGEFLKFGLGV
                     REMALGGAVVSDPTAVAAHYWNPATLTDNTCFSGNFMHTEEFAGAVALDHFALALPGK
                     GEYSFGAGYLRIGVDDIADTRNALLDLGTDGLGPGDEGYLGPDADGTEGNGRLDPGER
                     LDYGKFGSFGASENAVFLSGAKILQPKLKVGGTLKMLYRSLGDDKAYGIGFDIGVLYQ
                     PAPKINLAAAFNDLTTTFMLWNDGEREIVAPSLRIGGSVHHHFKKLPLAIEPLFGADI
                     LFEGKQNTADISIGGTVVRARIGLEVIYKECIALRAGRDDLGNPQIGLGLNTSVGSLD
                     YGLGLGGSYAEFGSSHRIALTLHFAELQRALKRYL"
     gene            12174..13499
                     /locus_tag="PLI55_05990"
     CDS             12174..13499
                     /locus_tag="PLI55_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00420.1"
                     /translation="MTSNNDLKSKNAMSIILLLTAIFFAMSCRECPTESENNIDLIVK
                     DIFCTSVVLKISLPYSGAVNTFALDRNDSTVATYTCSEDDTLIIDEGLTPATKYLYSV
                     RFLKDGKTKSESDPVAVHTMNITSHDISYWDIDTLGISGGIYDLWIVSEDNIWAVGQI
                     IAEDPDSSFDGSGEEWFNAAHWDGEKWNLIRILNTATLYSIWYFSESDIWVSSGFPKH
                     WDGQQWTMYHFQDMGLGIEVSTEHIWASSPNNVYFVGWKGSIVHYNGSNFTKMESGTT
                     CKLEDVWGIDGNHIWAVGTESDNSRSVILFYNGKTWNTLHDTNNERKCWSNSVWTDNP
                     YLLFLNGGSGRYLFDLMQEKLSKYESAGRWFGYDIYGTAYNDIFASTAGSEMLHYNGS
                     TWYLYPEVKAQFEDYFRIRAVKANDEIVVASGYYYTGIGGLPIIIRGYR"
     gene            13528..>15969
                     /locus_tag="PLI55_05995"
     CDS             13528..>15969
                     /locus_tag="PLI55_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00421.1"
                     /translation="MKKLLFLMLVIISISNLYGGVDLSNRNMTLRFNGHDHLVHDILS
                     DTMQAAYTAWKGRIQQQIYDYLDNKSGISNISVTVPSQISKITFYQGTSGNGQMNIRI
                     PNWYITFNYSACSFEADFDININAKMKTEVDTCYWLKIYDIINHGKINMDVEPREWYQ
                     FWNYPWCWAFEEIVLPFIYEYQFADQFEDEMRDMRVEIFPIKDLPGDCTDPNLVQEAI
                     NSFPIDFSFYNDSSPDSNLVICIDFMKGTQTNPNAFINIQPDPVNHDSYDHIGFACQF
                     DNFMYAYLNSSWPNHSIDNPIECATTMLNLMASTSAQSIRLEVPWLKIQSNIPTNKVN
                     CGLDPDFLDTPAGQEIINNFMNLSTWQDFDAVFSMIMNQKYEPILQIGLGHTAHVPRV
                     GSGLYMAPNNPIGQIDNTYFVDENTYLYYLKLFAHATVRRYCNQVAYWMIEGEFNVTK
                     FNHLFYGWRLGNLWDDETSGGFQDKVWDILVNAVRNEDPTAKIVANFHMLNLVKGLER
                     FGSDCDVIGVNLYPNMRFALPVLGFAPGELVWATRRALKGLGMGNKEVWVTETNYPAI
                     SSSNPPPDITLEEDLLYYSYSRQADYLQSAIETSLEFGAKGFFWFSFWLYEVKSYGDY
                     TEYGGLIPKNTLSLKQPTASRASTTTLNNHPGKCAVLLTNKNLTSGVNLGGKISLAGE
                     RDSLQSGEKPVYALRDRTHISRTDQRVLQGLVHNHWNTNDDFKLAESFEIGEDLQEYS
                     RVAYFTSTANITISTNLPQASIEFWDPWYYIEETQTQPDCYRTVSPGNFSVFTNQNPE
                     FSTTKPIYSLKVPQPH"
BASE COUNT         4548 a         3442 c         3415 g         4564 t
ORIGIN      
        1 ttttctcata ttaaagctcc attagatata tatcgcgaca gagcgaaaga aataatagtt
       61 cgtcacgcag taaaaaagac tgataaaact agtaacacaa agagaatagc cattctcgtt
      121 gtttttagag atatatttat ctgttctcat aggacccctt ccaataagtc tgccgctaca
      181 ttcatagcct tgttttaaaa tgatacggaa cctcctaagt catgtccgcc cgagctgcat
      241 tccccgccct tctcttcccg gtccggtaaa gccaatcgct aataatttgc ccgcgcgcca
      301 tattttaaaa tgccatcctg ttacaggcgt aatatatacg cagggttggg gctaatgtca
      361 agaaaaatat tttgtatttc ccgcagaacg aaggattaac ttaataatcg ggattatttt
      421 taactgcact gcatcctcct gcggcgtttt catataccgg aagtttgggt ttaatcgcta
      481 ctaaaccggg aaaccggcgc gccagacttt ccgcaaataa ataatccaga tgagcattcg
      541 attgcggcgc aaagccgcag acgtcttgcc agacagtcgg actctccata caaaaataaa
      601 caaatggtgg ctcgcgaatc tcggtcagtt tgcggtaaat ggaacggtat atttcggttc
      661 gccgcggacg aggatagcgc atcttttgat cttgaccacg aattagttca ccataaggta
      721 ggcgtgaata cgggtaacgc gcgattattt tagttttcat tgacggggga aaacgcaagg
      781 aaccgagact tatccaagca atcctggcgg gatcaacgta atggtataat tcttcgatta
      841 aatccagata ttgactttcc caatccggaa tatcaatgat cggatcaaaa tgaaaaccaa
      901 ttaggaaacc tcgccgagcg gcttcggcgg cggcttttag gcgccttgta ggtggaggcg
      961 ctaagtgctc ttcgctattt cttatgacta atggatttag cgaccaggaa atgaccgtgt
     1021 gaccattatg cggctgatct aatattaaac cgattcgatc ggttttggtt ttaagctcga
     1081 gcaaagcatt cttctgagtg gcaaagtatc ctatcaactt aatggcaaaa atttgggagg
     1141 cggtcaaagc taaactatcg ccgagttcgc cggtcccgaa gcgcaataaa cgatggggtt
     1201 gactggtaat cttctgttca atctcagcaa ttatcttttg gtaatcggta taaataattg
     1261 ttgccggctg attcaggtaa tattgcaaaa tacaataagt gcaattcaac gggcaattta
     1321 aggtttgatt aattacctga tagcgacagc agagatacgg cgcggctgta cccggacaat
     1381 ccttaactac ttcgccctta gaatgagtta ccagtaatat ttttttgggg tcttcctgcg
     1441 gaatcgtttc agttaaagat ttttccgatt ctattgctat ctttttttgt ttaggataag
     1501 cagtaaaaat ttgctcgact atttcatctc gagcacattc cgccgtaacg aatattttct
     1561 ttatcaagcg atcagccggc aggttcatcg gaattgcagt tcactattta cagtatttcc
     1621 agtaaatgct tcagatcgga ttgagcggca ctattcaaaa attcagtaag ttcccgcaga
     1681 tcgtttggcg tgcgaatttc tgccttaagt cggtaacctc gttcttcaaa attcgggtta
     1741 aattcaatct cgactgccga tggcaattga attgtttgca gaaaattatt taaccgctcc
     1801 cgatggatag tcatctgtgg aaaacggcgt tcctgaattt tactacggaa tatctcaagt
     1861 ttttgtgacg gttgaatttc ctcggctttt tctattaaaa cttggctcat ttctgctgtc
     1921 aactgttcag gagcaatgtc ctcgcggcgg agaatttcca gcaaattacg gctaatttct
     1981 tcaaaagtgc tcaacgacgg ctggcattca gtaattaaat tcaaaatcaa atctcgaatt
     2041 accggcgggc actcagccgt taaaaaccag gttttcaatg gcgctttttt ttcaattaaa
     2101 aacttatgca aaagtggatt gagcccgatc aatgctaaat agcgttcttc cagagtacga
     2161 gtcaaatctt tctcggtaaa ttttttaaaa aatgccgatt tcttggcatt ggaaaattgc
     2221 tgttgattaa atagctgtaa aatccgcgca atttcgattg gccaaagcgg acgcgagcgc
     2281 cgaataaaat ccgccactat attcaaggct gaaaaaagcg attgactttt aggaataaca
     2341 aacgcctgaa tgttttttcc cccacattga taaatattgg aaatataatt aaatccataa
     2401 aggatagtaa aaccatcgcg agcgtggtgt aaaaataccg gctgaaggaa aagcgattcg
     2461 ttcggtttat tacaatccgg attaatttcc tccacaaaca acctttcctg agattgtgac
     2521 gggattattt gttcaatggc aatttttttg atttgcaaga aaaacttcct ttttcttttg
     2581 gtaatcagat agtttgtcgg cataaatatt aggcaactcg ggatgtttag tgcgtccttt
     2641 gctcaagatc gtaagttttt taatgatttc atcgagtacc aattcagttt gttgccgatc
     2701 gagttcggcg tttaaaatct tctccaatgc cagcactcga taacgatcaa tgccccagta
     2761 tttacgcgaa agctggtcgg aatgaccacc agttttaatg attaactttt ccggtagtaa
     2821 tccaatttca tatttcaagg aaactcgcaa ccagagatca taatcttcgc aaaccggtaa
     2881 actttcatca aatactccga catcattaaa aaccttctga tgaacgataa ccgcggaagg
     2941 gctgacgata caaagcggca gacattggac aaaaatccaa ccggaatatt tttgatgctt
     3001 ctttgtcgga ttgactacga ccccattccg aatccatttc tcgtcggttt gcatcaccaa
     3061 ccactgcgga ttttccgcca aacattgcat ctgacgctca agtttacgcg gcaaccaata
     3121 atcatcagaa tcgagaaacg ctacccaggt gccttcggat ttttccagtc caagattacg
     3181 agcagtggac actcctgaat gcaattggga aaaataacga attttatttt tatacttatg
     3241 aattatgcgg ctggtattat ccgtactacc gtcgtcaata acaattaatt caaaattgcg
     3301 gtaagtctga ctcaaaaccg aaacaatggc gcgttccagg caggtttccc gattataagt
     3361 tgggattatg acagaaattt tctgcgggtc atccattttc agtcttaata tcaagtttat
     3421 tcagcaagaa atcaacaaaa attagtgcat tttgcgccag caattgagct gactcggcaa
     3481 gattggcatt gaaattttgc ttcacctcgg catctgcccg atccgtaatg actttcaggc
     3541 aggcaaaagg aattttatgt cgctggcaaa aatcagcata ggcgaaggct tccatatcaa
     3601 caacgtcagc ctgagaattc tgtaaaattg cccagcgctt cgcggctgaa gttaccggct
     3661 ccctactcga aaatagcgtt ccacttttcc agccagttga cagaaaatga ctagattcga
     3721 ggaaatcatt tggcagtgta agataggtca aatggtcgtt gctcaatgtg caaaaccgat
     3781 gcggataaaa gatactcatt agcggacaag tatcgttgag gacaccagcg gtaccgatat
     3841 taatgatagc taggtaatct tgacaaacga ctttttccgc caaacagcac cttactcttt
     3901 ccaagccaga accagttagg atataatcaa aatctatccc cgttggacta aaggaagcct
     3961 ggctgagttt ttcgattaat gggttaatct cggctttaaa agcggcacct atcagaatta
     4021 catctcgcga ttttgaacca gttaggacca ttgatacaat attaacgcct tgtggtcaat
     4081 tttggaaatt ttccacctaa tattgggaat attttaaaaa ttaattgggg aatcagacca
     4141 aattaggaaa caatcgcctc aatctgttca tcttgttcgg cagattcact cggagtagcc
     4201 ttagccggtt ttaaactggc actgataata tccttccaga cttctttggg taaggttata
     4261 taccagccgc aattattaca cactttgtaa taaaccttat tgccaaagta agtgcttttc
     4321 cgatagacga tgttgccgga acatttaggg cattgctcat taatcttgct gtcgtaaggt
     4381 tgggcgcttc cctttttccc actgttgtta aaattatcac tcatagtgat tgaacctttg
     4441 aaatttggtt tcttcggaca tttgacattt actactaaaa atcagtaaaa aattaatttt
     4501 ttattcttat tctctactgg tctaaatatt cgtatttcta atggtaattg tcaagcattt
     4561 aatttctaaa ctaacaaaaa attactgatt cgcccctttt acgagctata attcattttg
     4621 ttcacagaaa cgaatagtag tcaatttatt tttttccaat agaatatttt ctccaaaaag
     4681 tattgtgtag ttaggttaac ctaattaaat taggcagtat gaatgaaaaa caatctcacg
     4741 gacctaccat ctcccaagcg caggaagatt atctcaaaat tatcctcgag ctgattaatt
     4801 cccgccgcgt cgcccgcatt aaagaaattg cccagcggaa aaacgttagt atgccgaccg
     4861 tgaccgaagc tatgcggaaa atggcgcagg acggcttgat aaattactcc gcgcatgaat
     4921 ttattgacct tacctccgag ggtgaacaag ccgctcacca gatgaccgcc cggcataaat
     4981 ttcttcgtct tttccttgag gatatactga acatcccatc tgaaactgcc gacaccgaag
     5041 cctgtctcct cgagcatcac cttagtccaa taaccttaga gaggatgatt ttactctatc
     5101 agtttttagt gaactgtcct catcacgata gcaacctcat cgaagatttt aggcagtgtc
     5161 tgtctgcgga aaagcttgcg gataaaacca gttgtcgtga ttgttttgtc aaaatcgcat
     5221 ttccccatac cgttactaat ccttttgcag ttcattcgct attagccaat ctatcggaag
     5281 gacagaaagg gacggtaacg atgctcggtg ggaatacagc tatgcgacat aaattaattg
     5341 aacaagggct cgtccccggc gctacggttc aaatggaaag aaccggtaat aagagccagc
     5401 cttttgttat ttcagtcaac ggtcttcaaa taaagctcga tatagaacag gctaacctta
     5461 tcgaagtcgc aatatcttca ggagaaattt aataatgtcc acaaattcga ctactcttgt
     5521 agcccggagc ctagctgaag ttcccgccgg cagatgtttc agaataaaaa aagtccatgg
     5581 ccacggtgaa atcagacaac gtctgattga ccttggtttc attaaaaatg aggttggaac
     5641 ggttatccgt gaagctttgc taaaagatcc gatcgaggtc gagctcaagg gcactcactt
     5701 ttcactacgg cgcagtgaag cccgtttgat cgaggtcgag gtcatagatt gaattttccc
     5761 ccacaccgac accgccatcg ccgcggtcag cgccaaattt cgccggatag ttcccagaaa
     5821 gtaatccgaa ttgcattagc cggcaatccg aattcaggta aaacttcact tttcaatgcc
     5881 ataaccggcc agcatcaaca catcgggaat tatccggggg ttaccgttga gaaaaaatac
     5941 agccttttcc actataaaga cgatgtaatc gaaattattg atctgcccgg cacctacagt
     6001 ttgacggcct actcgttgga agaaatcgtt gcgcgcgatt tcgtcattaa agaaaaaccg
     6061 gatgtcatca ttgatgtgct ggacgcgact aacttggaac gacaactgta cctgctttta
     6121 caatttcagg aactcggtgt gcccttagtt ggtgctctaa atatgtctga cgaatgtgaa
     6181 aaaaagggta tagatattga ccatgaacga ctttcgtcaa ttctggggat tccactcgtt
     6241 aaaacagttg gtacccgcgg ctatggtata gattgtcttt tggataccgc tattcgagtt
     6301 gccaaaggca agctccgggc tgatcagcgc catctaaact acggcgaaga tgttgaaaat
     6361 gcccacgatc agattatcaa ggtgctggaa accgatccgg atttttctca taaatacccg
     6421 gtccactggc tggctatcaa actgctggaa aatgaccgcg atgccattca aaaggtcaaa
     6481 agcgaacata ctaatgctga aactgtttta ggcgaaagcg aacgcattcg acgtcaactc
     6541 gaaaatcatt tccacgacga tatcatctcg atcttagctg aaagacgtta cgcctacatc
     6601 aatggcgcta tccgcgaggc agtgcggatt gatcgaactc ggccggcaat tgattggact
     6661 gaacgaattg atcgggttat actcaatcgg tttctgggga tcccgatttt tctgctgatt
     6721 atgtttttga tataccaagc aactttcgga ttgggaaatc ccttagccgg tttgatatcg
     6781 aatagcttcg ctaacctggc agatttcgtt aaccttcatt taccagataa ccggtttcgt
     6841 gattttttga caaacggttt aatagcaggt gtgggaggcg ttctggtttt tctgccatta
     6901 gttgtattat taatgttggg actttctctg ctggaagata ccggttatat ggctcgagcg
     6961 gctttcgtga tggataaatt tttccattta ttcggtttgc acggtcggag ttttattccg
     7021 cttatgttag cgaccggctg tgcagttccc ggggtaatgg ccgctcgttc gctggctaat
     7081 agacgcgatc gaatcatcac cattatggta acaccattga tgatgtgtag tgctaaaacc
     7141 ccggtaatcg ccatgctggc ggcggctttt tttcccgaaa aagccgggct aattttctgg
     7201 ggcatctggt ttcttagctg gtttatggct ctaatcattg ctcttatttt ccgcaaaact
     7261 ttctttaagg gcgatcaaag tcctttcgtg atggaactgc ctccttaccg ctggccttct
     7321 tttcggagcg tgatgattca catgtggcag agaaccttag cctacctacg aaaagccggg
     7381 acgattattt tatttgccgc cattattgtc tggttcgcac ttcataatcc gacaaacccg
     7441 ggttcacact ctaccgatgc cagccaattt aatgtaaata ataatcaggg tagtgaaaca
     7501 gttaatcagg gtcagcaatt atatcatagt tacgccgggc aaattggtcg cggactcgaa
     7561 ccaattctaa aatgggctgg atttgactgg aaaatcggag tcagtttaat cgccggtctg
     7621 gcggcaaagg aagttattat ttccactctc ggaatgcttt acggcatccc agaaggagcc
     7681 caggaagcaa ctgaacaatc taaaatgacc accgtaagtt atcaaattag ccgggatcca
     7741 aattattcac ctctatcggc tttcagttta atgattttcg taatgctcta taccccttgc
     7801 ctagcgactt tggcggtggt caggaaagaa ttaggccaca taaaatgggc tctttttcaa
     7861 gccgtttata cagtaacgat tgccttcatt gcatctgttg ctattttcca gataggcaca
     7921 tttctgcaac acatattttg atatcaatgt tttcggtaaa aacaagctgt cttgaaaaaa
     7981 attctttcag cttactctta attattcaac tttgttcgga aaaaccggcg atttttagtg
     8041 agataaactc tgattttccc gttttcaaca ttgcgttact ttctgtatat ttcaatcgga
     8101 aatgaaatgc gatgactaaa tctacagaaa aaattgacga aaccactcca gttatgcgtc
     8161 aatataacgc cgtcaaggct caatatccgg acgcgctggt gcttttccgc atgggtgatt
     8221 tctacgagac tttccgcgaa gacgctaaac aagccgccaa aatactcaat attactctaa
     8281 ctaagcgcgc caatggcaaa gccgcggctg ttccgctggc aggatttcct taccacgccc
     8341 ttgagtcata tttacacaaa ttactgaaag ccggcctgaa agtcgctatc tgtgagcagg
     8401 tcgaggatcc caaactggct aaaggggtag tcaagcgcgc tgtggtcgaa atcatcactc
     8461 ccggcacggc tatttccgat cgcttcctcg aaaaagaaaa gaacaattac ctcgtctccg
     8521 tttatattaa tggagaaaat gccggacttg ccgctctgga catctccact ggtgaatttt
     8581 atctgagcga aatcccgact gccagaataa cggaagccgt gctcgatctc cacccggctg
     8641 aaatcatctg tccagaatca atccaaaaag aaatccaaac tctgttcacc aatcaggttt
     8701 tgcgcatcac tccgctcgag gattggattt tcaccttcga aactgcttat caaactctaa
     8761 tcgaacactt taaaaccgat tcgctcaaag gttttggttg tcaatctgcg gtgcacggca
     8821 ttaccgccgc cggcccgatt ttgcactatt tgcgccaaaa ttaccagacc aatctccagc
     8881 atatatattc tattcgcaat ctggcgaccg acgagacagt cctgattgac gatttcaccc
     8941 ggcgtaatct ggaaattttc cagactatgc aaagcggcga tagaaaaggt acgctaatgg
     9001 acgtcattga ccagactgtc agtcccatgg gtgtgcgttt gttaaccaaa tggttgattc
     9061 gaccattgcg tgatccagcc gctataaacc aacgactcga cggggtcgaa tttttataca
     9121 ataataccaa aatccgccac caattgcgcg aaatgttagc gcattgtggt gacttagagc
     9181 gcctgatctc gcgccttagc accaaccgcg cttccgctcg ggaaattgtc gctttaaaaa
     9241 ataccttaaa actcattccc ggtatccgtg accttcttcc gaatcaactg gctttttcgg
     9301 aaattaacag ccttctcaat ccactaccgg aattggtcga actcttagag cgcgccatta
     9361 aggacgagga tttacctcta agcatccacg aaggcggatt tattaaagac ggctattcgg
     9421 cggaattaga tgaatatcgt catatcgccc gccacggcaa agagtggatc gctcgcttgc
     9481 aggaaaccga acgccagaaa ctcggtattc cctcgctaaa agtcggctac aacaaagtct
     9541 tcggctatta cattgaagtc acccgtacgc acgccgaaaa aatccctccg gaatatatcc
     9601 gtaaacaaac actggtcaat aatgagcgct ttgtcactcc tgaattgaaa gaatatgagg
     9661 aaaaactgtt gaacgctgag gagcgcatca atcagatcga attcgagtta ttccaggatt
     9721 tgcgcaccaa gattttacag tttatcgaag caatccaaaa aaacgccggc gcgttagccc
     9781 aaattgatgt tttagccgga ctggcagaac tggcggaaaa aaatcgttac tgtcgcccgg
     9841 tcatcaatga cgggacagcg attgatatca aagccggacg tcatccagtt gtagagcatc
     9901 tgctaccgcc gggcgagaaa ttcatcgcca acgacctcca cattgacaat ctcagcgaac
     9961 aaattttaat tatcaccgga ccaaatatgg ctggcaaatc tacttacctg cgccagattg
    10021 ggctaatcgt tttaatggcg caaatcggta gctttgtgcc agccgattcc gccacgattg
    10081 gcgtagttga caaaatcttc actcgcgtcg gtgccagtga taatttagcc gccggtgaat
    10141 ccacttttct gatggaaatg cacgagaccg ccaatattct caataatgtg acgccgcatt
    10201 cactggtatt gctggacgaa attggccgcg gcacctcgac ttatgacggt atggcaatcg
    10261 cctggtcggt taccgagtat ttacacaatc aacccaacgt cgccgccaag acattatttg
    10321 ccactcatta ccacgaattg actgaattgg aaaaaatctt accacgcctg aaaaactata
    10381 atgtagccgt acgggaatac ggcgatcggg tcattttcct gcgtaaaatt gttcccggcg
    10441 gttgcgataa aagttacggc attcacgtcg ctcaaatggc tggcgttccg ccggaagtca
    10501 tcggccgcgc ccgagaaatt ttaactaatt tgagtcctga ggaaaaggtt ttacctacca
    10561 atcgcgagaa attccgccat ttgccggaaa aagatgccaa ccagctcggg ttgttcggtg
    10621 acgacctcaa attgaaaaaa atgctggcag aagttgatat tgataacctc acgccgctgg
    10681 aggctttgca aaaactcgct gaaatcaaaa agagcttggg tggttgaatg caattgcaat
    10741 tcagggaaca ttccgcgcat tacggccgta ttaattgcaa ttttatgctg agaagaattg
    10801 aatttaaaat agtcctgctc ggtctgctgg cttgggtttt attgttaaat ccggtaaatc
    10861 tcttagccat cggcgataaa tatgccggag aattcctcaa attcggcttg ggtgtgcggg
    10921 aaatggcgtt aggaggtgcg gttgtatctg atccgaccgc cgtggcggcg cattactgga
    10981 atccagctac cttgactgac aacacttgtt tctcgggcaa ttttatgcat accgaagaat
    11041 tcgccggtgc ggttgcgctc gatcattttg cgctggcatt acccggtaag ggcgaatatt
    11101 ccttcggcgc cggctatttg cggattgggg ttgatgatat tgccgatacg cgcaacgcct
    11161 tactcgatct tggaactgat ggtttaggac cgggtgatga aggctacctg ggaccggatg
    11221 ccgacggtac ggaaggcaat ggtagactcg atcccggcga gcgccttgac tacggtaaat
    11281 tcggttcgtt cggcgccagc gaaaacgcgg tttttctttc cggagccaaa attttgcaac
    11341 ctaaattaaa agttggcggc actctcaaga tgctctatcg ttcgctgggc gatgataaag
    11401 cctatggaat tggctttgat atcggcgtac tttatcagcc ggcgcctaaa atcaatctag
    11461 cggcggcgtt caatgactta acgacaacat ttatgctctg gaacgacggt gagcgcgaaa
    11521 tcgtcgcgcc aagtttgcgg attggcggct cggttcatca tcattttaaa aaattaccgc
    11581 tggcaattga acctctattt ggtgctgata ttttgttcga gggtaaacaa aatacggcgg
    11641 atatatctat cggcggtacg gttgtacggg cgcggatcgg actggaagta atttataaag
    11701 aatgtatagc tttgcgggct ggccgcgatg atcttggcaa tcctcaaatt ggtctcggac
    11761 tcaatacttc cgtcggcagt ttagattatg ggctggggct gggcggctcg tacgccgaat
    11821 tcggttcctc gcaccgcata gcgttgacgc tccacttcgc cgaattgcaa cgcgccctca
    11881 agcgctacct gtaaaatttt tctgacacgg ataacgagat tgacaggatt gtcctaattt
    11941 attagttata gttataattt ttcccttcgt ctcaacgcta ccatgttgag acgcaagaac
    12001 atacaactaa cggaggggaa tatgcaactc aaaattcctg tacatacccg cgtctgactg
    12061 tatcagcagt ccgggcgcgg gagctattcg ccagtggcgg aaacacacaa tggttgaaat
    12121 tacgctaaaa tgagtgtatt ttcaattgtg aaaagaacaa aaagggaaaa acgatgacct
    12181 ctaacaacga cctaaaatct aaaaatgcaa tgtcaatcat cttactgcta accgccatct
    12241 tttttgctat gtcctgccgc gaatgtccga ctgaatctga aaataacatt gatttgattg
    12301 ttaaagatat cttctgcacc tcagtagttc tgaaaataag tttaccttac tccggggctg
    12361 ttaacacctt tgcgctcgac cggaatgact cgactgtagc gacttatacc tgttctgagg
    12421 atgataccct gattattgat gaaggcttaa ctccggctac aaaatattta tatagcgtcc
    12481 gttttttgaa agatggcaag accaagtctg aatccgatcc ggtcgcggtg catactatga
    12541 atatcaccag ccacgacatt tcgtattggg acatcgatac cctagggatt tcgggcggca
    12601 tatatgatct gtggattgtc tcggaagata atatctgggc ggtcggtcag atcattgctg
    12661 aagatccgga ttcttccttt gacggctccg gagaagaatg gttcaacgcg gcgcactggg
    12721 acggtgaaaa atggaatctg attaggatat taaatacggc gactctatat agtatatggt
    12781 acttttctga aagtgatatc tgggtaagct ctggtttccc caaacattgg gatggacaac
    12841 aatggacaat gtatcatttc caagatatgg gattgggtat agaagtaagc actgaacata
    12901 tatgggcctc atctcctaat aatgtctact ttgttggctg gaaaggctct attgttcatt
    12961 ataatggttc gaactttact aagatggaaa gcggaacgac ttgcaagctg gaagatgtct
    13021 ggggaataga tggtaaccac atctgggcgg tgggaacaga atctgataac agccgaagtg
    13081 ttatcctttt ttataatggc aagacgtgga atactttaca tgataccaat aatgaaagga
    13141 aatgctggtc aaattcggtg tggactgata acccgtactt actttttctc aatggtggta
    13201 gtggcagata tttatttgat ctgatgcaag aaaaattgag taaatatgaa agcgctggaa
    13261 ggtggttcgg ttatgatatt tatggaactg cttataatga tatcttcgct tcaactgctg
    13321 gcagtgaaat gttacattat aatggctcga catggtatct ttatcctgaa gtaaaagcac
    13381 aatttgaaga ttattttcgg ataagggcgg ttaaagccaa cgatgaaata gtagtcgcta
    13441 gtggatatta ttacaccggc attggaggtc ttccaatcat tatcagaggg tatcgctgaa
    13501 aacctattaa ttaagaggat ttaaaaaatg aaaaaattat tattcttaat gcttgtgatt
    13561 atatctatat caaatctcta tggtggtgtt gatctatcca accggaatat gaccctgcgt
    13621 tttaatggac acgatcatct tgtgcacgat atactttctg atacaatgca agcagcatat
    13681 actgcttgga aaggaagaat tcaacaacaa atttatgatt atctcgataa taaaagtggc
    13741 atttccaata tttctgttac agttccatct caaatatcaa aaatcacatt ctatcaaggc
    13801 acttcgggaa acggacaaat gaatattcga attcctaatt ggtacatcac atttaattat
    13861 tctgcctgca gtttcgaagc tgattttgat attaatataa acgcaaaaat gaaaactgag
    13921 gtggatacat gttattggtt aaaaatttac gatattataa atcatggtaa aataaatatg
    13981 gatgtagaac caagagagtg gtatcaattc tggaattatc catggtgctg ggcttttgaa
    14041 gaaatagtat taccgtttat ttatgaatat caatttgcgg atcagttcga agatgaaatg
    14101 cgggatatga gagttgaaat atttccaata aaagatcttc ctggagattg tactgatccg
    14161 aatttagttc aagaggctat taacagtttt cctatagatt tttcttttta taatgatagt
    14221 tctccggaca gcaatcttgt aatttgcatt gattttatga aagggacaca gactaatccc
    14281 aatgctttta ttaatattca acctgatcct gtcaaccatg attcatatga tcatattggt
    14341 tttgcctgtc aattcgataa ttttatgtat gcttatttga atagctcctg gcctaatcac
    14401 agcattgata atcccattga atgtgccacc accatgctaa atttaatggc aagtacatcc
    14461 gcccaatcca ttagactcga agtaccctgg cttaaaatac agagtaatat tccaaccaat
    14521 aaagttaatt gcggtctgga tccagatttt cttgatacac ccgcaggcca agaaataatt
    14581 aataacttta tgaatctttc tacctggcag gattttgatg ctgtgttttc aatgattatg
    14641 aatcaaaagt atgaacccat attacaaatc gggcttggcc acactgccca tgtgccccga
    14701 gttggttcag gtctttacat ggcaccaaat aatccgatag gtcaaattga taatacctac
    14761 tttgtggatg aaaacaccta tttatattac ttgaaactct ttgcgcatgc gacggttcga
    14821 agatattgca accaggttgc atattggatg atagagggcg aattcaatgt cactaaattt
    14881 aatcatctgt tttatggctg gcgcttagga aatctctggg acgacgaaac ctcaggcggt
    14941 tttcaggata aggtatggga tattttagta aacgctgttc gtaacgaaga ccctaccgca
    15001 aaaattgttg ctaatttcca tatgctaaat ttagtcaagg gattagaacg attcggctct
    15061 gattgcgatg ttatcggtgt caatctttac ccaaatatga ggtttgccct acccgttttg
    15121 ggttttgcgc cgggcgaatt ggtttgggca actcgaaggg ctttaaaagg tttaggcatg
    15181 ggtaataaag aagtctgggt gacggaaacg aattatccgg ctatatccag ttccaatcct
    15241 cctcctgata ttaccttaga agaagatctt ttatattatt cctattctag gcaggcagat
    15301 tatttacaat ccgctatcga gacttcactt gaatttggtg ctaaaggttt tttctggttc
    15361 tcattttggt tgtatgaagt aaagagttat ggtgattata ccgaatatgg tggattaatt
    15421 cccaaaaata ctctaagctt gaagcaacct accgcttcaa gggcgtcgac aaccaccctg
    15481 aataatcacc cggggaaatg cgctgttttg cttacgaata agaacctgac ttcgggagta
    15541 aatcttggcg ggaaaatatc attagccggc gagagggatt cattgcaatc gggagagaag
    15601 ccagtatatg cgctacgaga tagaactcat atttcccgaa ccgatcaacg tgtgttgcag
    15661 ggattagtac ataatcactg gaataccaat gatgatttta aacttgctga atcattcgaa
    15721 attggagaag atttgcaaga gtattctcgt gttgcatact tcacttcaac agctaatata
    15781 acaatcagta ctaatctacc acaagccagt atcgagttct gggatccgtg gtattatatt
    15841 gaagaaacac agactcagcc cgactgttac cggactgtct caccgggaaa tttttcagtg
    15901 ttcacaaatc agaatcctga gttcagtaca actaaaccca tttacagcct gaaggttcct
    15961 caaccccat
//
LOCUS       DAOOMU010000048        15814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_3922_length_15814_cov_22.064408, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000048 DAOOMU010000000
VERSION     DAOOMU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15814
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3922_length_15814_cov_22.064408"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /gene="lepA"
                     /locus_tag="PLI55_06000"
     CDS             complement(<1..1526)
                     /gene="lepA"
                     /locus_tag="PLI55_06000"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPB00422.1"
                     /translation="MELNIRNFCIIAHIDHGKSTLADRMLEFTGTISRREMKDQVLDN
                     MDLERERGITIKSHPIQMNYHAPDGKNYILNLIDTPGHVDFSYEVSRSLAACEGALLL
                     VDASQGVEAQTVSNAYLAIENDLAIIPVINKIDLPGAMIPEAKRQIIDLIGCDENEII
                     LTSAKSGVGIEEIFEAIIKRIPPPKYEPDKPLRALIFDSVFDSFRGAIPYVRIFEGEV
                     RPGDWIRFYANNSTHEVSEVGIFKMQRQKTEKLSSGDVAYLVAGVKDISNIKVGDTIT
                     LKNNPASEPLPGYRVIKPVVFSGLYPVAAEDFEDLRSSLEKMKLNDSSLIYEPETSTA
                     LGFGFRCGYLGLLHMEIVQERLEREFNLNLITTMPNVEYQALLKTGELIEIDNPTKMP
                     AMGEIAEIREPYVRAEIIVPTTYLGNVLKLCQDKRGIYKTTKYLDITKVQIQYELPLA
                     EIVFDFYDKLKSVSRGYASFDYEPIGYRAGDLVKVDILISGEPVDALSFIVHRDKAYA
                     "
     gene            1660..2508
                     /gene="thyX"
                     /locus_tag="PLI55_06005"
     CDS             1660..2508
                     /gene="thyX"
                     /locus_tag="PLI55_06005"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPB00423.1"
                     /translation="MSHVVVPEAEALLDKEIKVLDHGFIRLVDYMGGDSRIVQSARVS
                     YGKGTKSVREDEALIDYLMRHQHTSPFEQVVLTFHCKMPIFIARQWIRHRAARVNEIS
                     GRYSVMEDEFYLPEETAIQYQNKDNRQGRDPQEVPVHLRQKVLNILAKGQQVAYDDYQ
                     KMLADDIARELARINLPLSLYTQWYWQIDLHNLFHFLELRMDEHAQWEIRQYANVIAD
                     ITRAVAPLAFRAFETHVVNAVRFSGSEIQALAANLRGQEHDLKGSRKIEYEAKLRKII
                     GNPGGE"
     gene            2674..4434
                     /locus_tag="PLI55_06010"
     CDS             2674..4434
                     /locus_tag="PLI55_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HPB00424.1"
                     /translation="MSKFQSKIFDNKIVIGVRDWVAEDVEELLQSEIFKDLLNRFLDR
                     LEKKQSPLLDIFGHYPIDDNDRRILYETLCYLTKLESKLVPNIVSDSQRLFADRHLLN
                     EFVEQLYNYWRSFDRFILCDSTGDNFDKRPYRTFNLTVERLTHLVRQAYRDIQENITA
                     QHPRVYRQVHAGAEVATIAVESHSSRFKAEYSQLGSISVIRQVLIYPPLILEPPMNKR
                     KGEFIEVTENPIKSVQFNTQEWLCYPAKVGSLIVYVYFHESFYELGFALCNLFELASD
                     RDLRRKPDGIFLYGVPEENLRHLADFPTVFYEDKANDLFVGAVPRGNEFGYFGYLKKM
                     ILTLHNLRVMQKGHLPFHGAFVQILLKNGTQANVLMVGDTGAGKSETLEALRCLGDEY
                     IKDLTIIADDMGSLVIQDQVVKAYGTEIGAFLRLDDLQPGYAFGQLDRAIIMNASKTN
                     ARIVLPVTSYENIIKGVPVDYIFYANNYENVDADHPVIEKFTTPDEALYVFREGTVMS
                     KGTTTTTGLVHSYFANIFGPAQRKAIHDKIARRYFVHFFEHKVFVGQLRTRLGVAGFE
                     IEGPKIAAEELLKILLTPSN"
     gene            4743..6221
                     /gene="trpE"
                     /locus_tag="PLI55_06015"
     CDS             4743..6221
                     /gene="trpE"
                     /locus_tag="PLI55_06015"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013278818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HPB00425.1"
                     /translation="MNYEDFEKLARPGLIIPVYRKFNADFITPVIAYLKLREAGKHSF
                     LLESVIKGEQLGRYSFLSQEPYLILKCRDDKTISIADHRSKIHRENFFTILQQQLKDY
                     ELLKIENLPRFTCGAVGYIGYEMAGQIEKLPKPKRDVIGSDDALLGFYNQLVAFDHLK
                     NEVILIANVFIDNDSDVRQLYLEAQNRLDCLQARLNRSYEGELRFTADLASEEANFTK
                     KDFMTAVEQAKEYILAGDIFQAVISQRFSMAYSGDVFQVYRALRNINPSPYMFFLDFL
                     DYQVIGSSPEPLIRAQDNELEIIPIAGTRQRGATSAEDDRLEAELLNDPKELAEHVML
                     VDLARNDLGRVATFGTVKVNDFKTIQKYSHVMHIISQVHGHLRDGLTVVDAFKSAFPA
                     GTVSGAPKIRAMEIINELEPEKRGIYSGAIGYFNYGGNLDLCIGIRTIVAGRKRLYYQ
                     SGAGIVADSQPENEYQETLNKCQALRQAIEQASGGINDFIHR"
     gene            6202..6798
                     /locus_tag="PLI55_06020"
     CDS             6202..6798
                     /locus_tag="PLI55_06020"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPB00426.1"
                     /translation="MILFIDNYDSFTYNLVDYIGQLHNDVLVVRNDQINVAEIENLKP
                     DGIVISPGPGRPEQAGFSCQIIQTYCHKIPILGVCLGLQAIAQIFGANIVRAPFPVHG
                     KASIVEHNGQGIFQGMTSPMTAGRYHSLIIERESLPECFTITAESSDHLIMAIAHRKF
                     PLFGVQFHPESVLTLNGMQILRNWVKIADKFKNNTEEV"
     gene            6800..7825
                     /gene="trpD"
                     /locus_tag="PLI55_06025"
     CDS             6800..7825
                     /gene="trpD"
                     /locus_tag="PLI55_06025"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPB00427.1"
                     /translation="MIKEYLEKISQCEDLTRQEAATALQMLIDNQLTATEIGALLFGL
                     RTKGESVEEILGFVDTMQANMVAVAIDDPDAIDVCGTGGDQSHSFNVSTTAAFVVAAG
                     GVTVAKHGNRSVSSKAGSADVLEKLGARIDLSPENTRRCLNEIGISFLFAPMYHPAMK
                     AVAPHRRNLGIRTIFNMLGPLINPANLKRQLIGTYNLETAAKTAAVLRQRCYKKACVV
                     HSEEGFDEASPFAGNRVFEVDIARPDIYEYFYRAEKCSQNLNSYQLVQGGDAEKNARI
                     TLEILNGRRGYPREMTALNAAFGLYVAGKVDNIPDGIFLAEELIDSGKALQKLNQFIE
                     MSSKLAN"
     gene            7836..8633
                     /gene="trpC"
                     /locus_tag="PLI55_06030"
     CDS             7836..8633
                     /gene="trpC"
                     /locus_tag="PLI55_06030"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPB00428.1"
                     /translation="MNWLDTIVAHKKQELIDAKKAVPLEFYQAQIGDNCSNHSLLRTI
                     KQQGGFHFIGEVKRASPSRGIINSDFDPLQQAKCYAKGGASAISILTDSQFFMGNLAD
                     LQVIREQIDLPLLRKDFIVDPYQIYESRYYQANIVLLIARILSPKQITQFCELATKLA
                     LEVLLELATENDLVKIPSNHSNLILAINNRDLANFEVDLNNSFRLKPLLPDDIPVISA
                     SGISNTDECVRLFKNGFAGALIGESLMRDREPDSLLKKFRSAIANAG"
     gene            8623..9252
                     /locus_tag="PLI55_06035"
     CDS             8623..9252
                     /locus_tag="PLI55_06035"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPB00429.1"
                     /translation="MPVKLKICGITSLEDALLAAQLGADWLGFNFYPASPRYIAPELA
                     AEIIKVLPRTVESVGVLVKPFLEEARKIITITGVQRLQIYEPQDFNDYSQLGVPVIAC
                     YRCKNGKITELNYASADMVLLDNYAESIFGGTGQAFNWRWIPTELERKKLVLAGGIDT
                     TNILEALEMIGPAVIDVASGSESKPGHKDPEKIKTLVSQIRIFNQRKDN"
     gene            9257..10432
                     /gene="trpB"
                     /locus_tag="PLI55_06040"
     CDS             9257..10432
                     /gene="trpB"
                     /locus_tag="PLI55_06040"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPB00430.1"
                     /translation="MEKGYFGEYGGQYVPEILRPALVELEEIYLALKDDPDFQKRLNI
                     ELHDYAGRPTPLYFAENLTRHYGQAKIYLKREDLNHTGAHKINNAIGQILLAQAMHKK
                     RIIAETGAGQHGVAVATVAARAGLECCIYMGAEDIQRQYPNVRRMQLLGAEVRPVHTG
                     TATLKDATNEALRDWLANVKTTHYIIGSVVGPHPFPTIVRDFQKIIGEETREQIVEKT
                     GRLPDYILACVGGGSNAMGIFYPFISDTAVNLIGVEAAGEGLASGKHSATLSCGSLGI
                     FHGMKSFVLQNNDGQIQLPYSLSAGLDYPGVGPEHCHLKDSGRVKYYAITDTEALAAV
                     ELLCRLEGIIPALESAHALAYLEYLLPQTHSRESVVVNLSGRGDKDLETYLKYLERR"
     gene            10442..>10657
                     /locus_tag="PLI55_06045"
     CDS             10442..>10657
                     /locus_tag="PLI55_06045"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPB00431.1"
                     /translation="MSTRLNQILKSKKEKQEKCLSIFLTAGFPEKNCTPEIILTLAAS
                     GVDFIELGIPFSDPIADGPVIQKASDKA"
     assembly_gap    10658..10757
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10758..11111
                     /locus_tag="PLI55_06050"
     CDS             <10758..11111
                     /locus_tag="PLI55_06050"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPB00432.1"
                     /translation="LDLIHLIAPNTPTERIRQIDEISTAFIYCVAYTGVTGQDNRPTP
                     ETIAFFNHLKQTLWHPWLIGFGVKSHEDFQTYTHYADGVIIGSAFINLLENTPENNRL
                     AACRTFVQNIINSPS"
     gene            11265..12611
                     /locus_tag="PLI55_06055"
     CDS             11265..12611
                     /locus_tag="PLI55_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HPB00433.1"
                     /translation="MKIVKSYAEINQKIKNRQAVVMTAEEIKSFIREQGIKSVLEKVD
                     VVTTGTFGAMCSSGAFLNFGHTDPPLKMEKVKINDVPAYGGLAAVDAYIGATERSQKS
                     NGHYGGGHVIEDMVAGKNLVLEAEGKPTDCYPLDSIVTEFTLKDLNQAVLLNPRNAYQ
                     RYNAAANSTDRIIYTYMGRLLPRLGNVTYSGAGELSPLNNDPNFLAIGLGTRIFLGGG
                     VGYVIDSGSQHSPETGFANLMVKGDLKLMRPEYLRGAYVYGYGCTLFVGIGVPIPLLN
                     EEIARSVAITDDELRVNIVDYGIPRLKRPILRTGVTYRELKSGQVEIEGKPVPTGSLS
                     SLFMARKIANELKNWILNGNFLLTEAIEPIPKIGKAKQLAVKPPKKPQAISTTNQKHS
                     NGIQIDHSRCILCELCIAQCPEKAIYLNENNQITIDQAKCNLCRICTKVCSVGCLE"
     gene            12625..13338
                     /locus_tag="PLI55_06060"
     CDS             12625..13338
                     /locus_tag="PLI55_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HPB00434.1"
                     /translation="MNQTVTFRYKNTIARITGEPELVKIGEEEIIRCYTDIENYISND
                     PIFKATLKPYKVPNDASKIIREMATAGAICGVGPMAAVAGAIAKYAVLAMQKAGATFA
                     IVDNGGDIAILNDRPVVVGIYSGPAVIHDIGLRIPVHSTLTSVCTSSGVIGHALSFGK
                     SHASVILADDPVLADAAATALGNRIREKDKFEIENAMNEVLKFGVAGCIVIIEDYVGF
                     AGEIPEICRVEMSESMIAK"
     gene            13355..14734
                     /locus_tag="PLI55_06065"
     CDS             13355..14734
                     /locus_tag="PLI55_06065"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPB00435.1"
                     /translation="MKVLKFGGASLKDKNAYAETIRILAEDKEPKAVVLSAIYGMTDW
                     LETTIREARANEQSIENTTTKIRAWHAKILTDLVTDPTMISDCMNELEERIQKLERLL
                     YGLAYTEEMTPQLYALIMSFGERLSVILSATIFKAHGIPAQYLESDKIGIVTDDSFTS
                     ATALLPIVRKNLPSHVYPLLEKGITPLITGFFGVNKNGKVTLFGRNSSDYSAAVIAQG
                     LKADRLEIWKDVDGFMTADPKIVPTATLIDKLSYYEAAELSYFGAKILHPRTVEPLIH
                     DSIPLVIRNIYHPEIEGTEICAKEILNTAIIKSVTYNKNISVLRIKGAGVGYKPGVIS
                     EIGSRLAACGVNIYSIITSQTCINLLIDRKDSKVGYKALQVLKDGLIDSIEHCDDEVV
                     VAAVGAGLKQTPGLAAKIFSAIAGQNINIEMISLGASDVATYFIVKEPCLVPAVNALH
                     TAFFTNSIN"
     gene            14745..>15814
                     /gene="asd"
                     /locus_tag="PLI55_06070"
     CDS             14745..>15814
                     /gene="asd"
                     /locus_tag="PLI55_06070"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPB00436.1"
                     /translation="MKSNSQLKSKIPVGILGATGSVGQKFIELLTEHPWFEIAALAAS
                     DRSAGKTYRDAAHWFMAKPLPDNVAKMIVKNCEPNLPCRIVFSGLDASVAGEIETDFA
                     KAGYIVISNSRNHRYDPDVPILVPEVNSDHLAIIATQKYGRGAIITNPNCAATGLVVA
                     LKPLLDQFGLSAVNIVTMQAISGAGYPGVASLDIVDNVIPFISGEEAKLESEPQKILG
                     KYLDGHIEPAQFIISAACNRVAVVDGHTECVSVKLERKPTAGEIIAAWREFKGEPQRL
                     KLPSAPEKPLCYFEESSYPQPRLHRLLENGMTVSLGRLRECPILDYKFVMLSHNTVRG
                     AAGGAILNAELFIAKGGLEKLR"
BASE COUNT         4447 a         3242 c         3667 g         4358 t
ORIGIN      
        1 tgagcatagg ctttgtcgcg atgaactatg aacgatagcg catcaaccgg ctcgccagaa
       61 attaaaatgt caactttcac taggtcaccg gctcggtatc caattggctc gtaatcgaag
      121 gaagcataac cgcgcgatac tgacttgagt ttatcgtaaa aatcgaaaac gatttcagct
      181 aagggcaatt catactgaat ttgcactttc gtaatgtcga ggtatttagt tgttttgtaa
      241 ataccacgtt tatcctgaca aagtttcagg acgttcccca agtaggttgt tggtacgata
      301 atttcagccc gtacgtatgg ctcgcgtatt tcggcaatct cgcccattgc cggcattttg
      361 gttggattat ctatttcgat aagttcgccg gttttcagta aagcctgata ctcgacattc
      421 ggcatcgtgg ttatcaaatt caggttgaat tcgcgttcca gacgctcctg aacgatttcc
      481 atatgtaata gtcctaagta gccacagcgg aagccaaaac cgagtgccgt tgaagtttcc
      541 ggttcgtaaa tcaagctgga gtcatttagt ttcatcttct ccagcgatga acgcaaatct
      601 tcaaaatctt cagccgctac cggatacaag ccgctaaaaa ctactggctt aatgacccga
      661 tagccgggca gaggttcact cgccggattg tttttcaggg taatggtgtc gccgacctta
      721 atattcgaaa tatctttgac tccagctacc aaataagcga cgtcgccgga acttaatttt
      781 tcagtttttt gcctctgcat tttgaatatg ccgacttcgg aaacctcgtg ggtggagttg
      841 tttgcataga accgaatcca atcacctgga cgcacctcac cttcgaaaat ccggacataa
      901 gggatggcgc cgcgaaatga atcaaaaact gaatcgaaaa tcaaggctct taacggctta
      961 tccggttcgt atttaggtgg tggaatgcgc ttaatgattg cttcgaaaat ctcttcaatg
     1021 ccaacaccgc tcttagcgct ggtcaggatt atttcgtttt catcacagcc gatcaaatca
     1081 atgatttgac gtttggcttc cggaatcatt gctcccggca aatctatttt atttattacc
     1141 ggaataatag ccaggtcatt ttcgatggcg agataagcat tgctgaccgt ttgagcttca
     1201 actccctggg aggcatctac caaaagcaaa gcgccttcac aagccgccag actccgcgat
     1261 acttcatagg aaaagtccac gtgtcccgga gtatcaatca aattcaaaat atagttttta
     1321 ccatcggggg cgtgataatt catctggatc ggatgactct tgatggtaat accgcgttcg
     1381 cgttccaagt ccatattatc caggacttga tccttcattt cacgtcggct gatagtaccg
     1441 gtaaattcga gcatccgatc ggccagagtt gatttcccat ggtcaatgtg agcgataatg
     1501 caaaagttac gaatattcaa ttccacgtcg gagaatttac ggcagataca gcctgattaa
     1561 caaaaattcc aatataattt ttaatgtatt ttaaaaaatt cggatttcgg taataataaa
     1621 aatcctaaat ttccgaggtt tagaataagg agaatttaaa tgtcgcatgt ggtagttccg
     1681 gaagccgaag cacttctgga caaggaaatc aaagttctcg atcacggatt tattcgccta
     1741 gtggactata tgggaggcga cagtcggatt gtgcaaagcg ctcgggtttc atatggcaag
     1801 ggaaccaaat cggtgcgaga agacgaggcg ctcattgatt acttaatgcg tcaccagcat
     1861 acttcgcctt tcgagcaggt ggttttaaca tttcattgca aaatgccaat tttcattgct
     1921 cgacaatgga ttcgccatcg cgcggcacgc gtcaatgaga tttctggacg ttatagcgtt
     1981 atggaggacg aattttatct ccctgaggaa accgctattc aatatcaaaa taaagacaat
     2041 cgccaaggac gcgatccgca ggaagtacct gttcatctaa ggcagaaagt tctgaacatt
     2101 ctagccaaag gccagcaagt ggcttatgac gactatcaaa aaatgttagc tgacgatatt
     2161 gcgcgagaat tggcgcgcat aaatctgccg ctttcgttat atactcaatg gtactggcag
     2221 attgacctcc ataatctttt ccactttctg gaattgcgga tggatgaaca cgcccagtgg
     2281 gaaatacgac aatacgctaa tgtaatagcc gatattacgc gggcagttgc accgctggca
     2341 ttcagggctt tcgaaacgca tgttgtcaat gccgttcgat tctccggctc ggaaattcag
     2401 gctttagccg cgaatttgcg cggtcaggaa cacgacttga aaggtagtcg caaaatcgaa
     2461 tacgaagcca aactgcgtaa aattatcggt aacccgggcg gggaataata atatccattg
     2521 tttacgaaat caagtcgttt ctaataataa aaataatgga ttagcaagta ctgatgcagg
     2581 ttaaaataat tggaatacat ttgcaaaata atcgagtgat actaaattaa ccggttaact
     2641 tgattatttt tatttagaaa aggctgtgct gttatgagta aatttcaatc gaaaattttc
     2701 gataataaaa tcgttatagg cgttcgagac tgggttgccg aggacgttga ggaactcctg
     2761 cagagtgaaa ttttcaagga tttactgaat cgtttcctcg accgcctgga aaagaagcag
     2821 tcgcccctgc tggatatttt cggacattat ccaatagatg acaatgatcg tagaattctt
     2881 tatgaaacat tatgttatct taccaaattg gagagtaaac tggttccgaa tattgtttct
     2941 gatagccaac gattattcgc cgaccggcat ttgctgaatg aattcgtcga acaattgtac
     3001 aattactggc gcagtttcga ccgttttatc ctttgtgatt caaccggcga taatttcgac
     3061 aaacgaccat atcgcacttt taatttgact gtcgaacgat tgacccattt ggttcgccag
     3121 gcttatcgcg atattcagga aaatattacc gcccaacatc cgcgagttta ccgacaggtg
     3181 cacgccgggg cagaagtagc cacaattgcg gtcgaatctc atagttcgcg ttttaaagcc
     3241 gagtatagcc agttaggctc tatctcggta atccgacaag tcttgattta tccaccgctg
     3301 attctggaac caccaatgaa caagcgcaaa ggtgaattta ttgaagttac cgaaaacccg
     3361 atcaagtcgg tacaattcaa tactcaggaa tggctctgct atccggctaa agttggctcg
     3421 ctgattgttt atgtctattt tcacgagtcg ttttacgagt tgggattcgc cctttgtaat
     3481 cttttcgagc tggcgagtga tcgggatctt cgtcggaaac cggatggtat ttttttgtat
     3541 ggagtgccgg aagaaaacct gcgccattta gccgactttc cgacagtttt ttacgaagat
     3601 aaagccaacg acctattcgt cggcgcggta ccgcgcggga atgaatttgg ttattttggc
     3661 tatctcaaaa aaatgatact gactctgcat aacttgagag tgatgcagaa agggcatttg
     3721 cctttccatg gagctttcgt gcaaattttg ctgaaaaatg gaactcaggc taatgtactg
     3781 atggtcggcg ataccggtgc tggtaaatcc gaaacattag aggctttacg ctgtctcggt
     3841 gatgaatata ttaaagactt gaccatcatt gccgatgata tgggctcgct ggttattcaa
     3901 gatcaagttg taaaagctta tggcactgaa atcggagctt tcctgcgact ggatgatttg
     3961 caacccggct atgcttttgg gcagctagac cgggctatca ttatgaatgc cagcaaaact
     4021 aacgctcgca ttgtcctgcc ggttacttct tatgaaaata tcattaaggg cgtaccggtt
     4081 gattatattt tctatgctaa taattatgaa aatgttgatg ccgaccatcc cgtaattgaa
     4141 aaatttacca ctcccgatga ggctttatac gttttccggg aaggtacggt tatgagtaaa
     4201 gggacaacta ctaccaccgg attggtccat agctatttcg ccaatatttt tggaccggct
     4261 cagcgtaaag ccattcatga taaaattgcg cggcgttact ttgttcattt ttttgaacat
     4321 aaggttttcg ttgggcaatt gcgaacgcgg cttggggttg cgggatttga gattgagggt
     4381 ccgaaaattg ccgctgagga gttgctaaaa attttgttaa ccccatccaa ttaatagttt
     4441 taatatttta agccaaatac ttaatagatt gaagttagat cgaaaattaa aatagaatca
     4501 aaaaaacttc ttgactacaa aagagaattt gtttaaaatt gccacgtatt tttagagaat
     4561 tatagatagt atgcgaatgc aaatgaacat aaactggtgg tggcaaatga tggataagtg
     4621 tcattttctc aggtttatat atgagtaaat aaagaataaa tatacataaa aattatgaaa
     4681 cccgcctgag aaatgaccgg cgggtttttc attttataag gaaaaagcaa aacgagagaa
     4741 atatgaatta tgaggatttt gaaaaattag ccaggccggg tttgattatt cccgtttata
     4801 ggaaattcaa tgccgatttt attacgccgg tgattgctta cctgaaatta cgcgaagctg
     4861 ggaaacattc ctttttactg gaaagtgtca ttaaaggcga acagttgggg cgttattcat
     4921 tcctgagtca ggagccttat ttaattttga aatgccgcga tgacaagacg atttcaatcg
     4981 ccgaccatag aagtaaaatt caccgagaaa atttcttcac tattctgcaa cagcagttaa
     5041 aagattatga acttctgaag attgaaaact tgccgcgttt tacctgtgga gcggtcggat
     5101 atatcggtta cgaaatggca ggtcagatcg aaaaattgcc gaagcccaag cgcgatgtaa
     5161 tcggaagcga cgacgctctg ctgggctttt acaatcagct ggtggctttc gatcatttga
     5221 aaaatgaagt cattctgatc gctaatgttt ttattgacaa tgacagtgat gttcgacagt
     5281 tgtatctcga agcccaaaac cgtctggatt gtctgcaagc aagattaaat cgcagctatg
     5341 aaggtgaact gagatttacg gctgatctgg cgagcgagga agccaatttt accaaaaagg
     5401 attttatgac tgccgttgag caggctaagg aatatattct ggcaggcgat atttttcaag
     5461 ccgtgatttc tcagcgtttt tcgatggctt attccggcga tgtttttcaa gtctatcggg
     5521 cattgcgcaa cattaatcca tcgccatata tgttttttct ggactttttg gattatcagg
     5581 taatcggttc ctcaccggaa cctttgatcc gggctcagga taatgagctg gaaatcattc
     5641 caatcgccgg aactcgtcag cgaggggcaa cttccgctga agatgatcgc ctggaggctg
     5701 aactactgaa tgatcccaaa gagctggctg aacatgttat gctggtggat ttagcccgta
     5761 atgacctcgg ccgggtagcg acattcggca ctgttaaagt caatgatttt aaaactattc
     5821 aaaaatattc gcacgttatg cacatcattt ctcaagtgca cggtcatttg cgcgatggtt
     5881 tgactgtggt agatgccttt aagtcggctt ttccagccgg aacggtttcc ggcgcgccta
     5941 aaattcgggc aatggaaatt attaatgaac ttgaaccgga aaagcgcgga atttactccg
     6001 gagctatcgg ttatttcaat tatggtggca atctggattt gtgtatcggc atccgcacca
     6061 tcgtcgccgg tcggaaacga ctatattacc agagtggagc gggaatcgtc gcggatagcc
     6121 agcccgaaaa tgaataccaa gaaactctca ataaatgcca ggctctccga caggcaatag
     6181 aacaagctag tggaggaatc aatgatttta ttcatagata actatgattc tttcacttat
     6241 aaccttgtgg attacattgg tcaactgcac aacgatgtgc tagtcgtgag aaatgaccaa
     6301 ataaatgttg cggagatcga aaacttaaaa ccggatggga ttgtcatttc tccgggaccc
     6361 ggtcggccgg aacaagccgg attttcctgt cagataattc aaacttattg ccataaaatt
     6421 cctattctgg gagtatgcct cggattacag gcgatcgctc agatattcgg ggctaatatc
     6481 gttcgggcac cctttccagt gcatggcaag gcttcgattg tcgaacataa cggtcaggga
     6541 atttttcaag gaatgacttc gcccatgacg gccggtcgct atcactcact gataattgag
     6601 cgtgaatctt tgccggaatg ctttacgatc acagctgaaa gcagtgatca tctcataatg
     6661 gcaattgctc accgcaaatt tcccctcttc ggagtccaat ttcatcccga atcggtattg
     6721 accctaaatg gaatgcaaat actacgcaac tgggtaaaga ttgcggacaa atttaaaaat
     6781 aatacagaag aggtgtaata tgatcaaaga atatcttgag aaaataagtc aatgtgaaga
     6841 ccttactcgc caggaagccg ctacggcttt gcaaatgttg attgataatc agttgactgc
     6901 caccgaaatc ggtgctctat tatttggatt gcgcacgaaa ggcgaatcgg tagaggaaat
     6961 tctgggattc gttgacacga tgcaagccaa tatggtggcg gtcgcaattg atgatccaga
     7021 tgccattgat gtttgtggca cgggaggtga tcagagtcat tcatttaatg tttcaacgac
     7081 cgcggctttt gtggtggcgg ctggtggagt taccgttgcc aagcatggta atcgctcggt
     7141 ctccagcaaa gccggctccg cggatgttct ggaaaagttg ggcgcccgga ttgatctttc
     7201 tccggaaaat actcgcagat gtctgaatga aatcgggatc agttttttat ttgcgccgat
     7261 gtatcatccg gcaatgaaag ccgtagctcc gcaccgtcga aacttaggaa ttcgcacgat
     7321 tttcaatatg cttggaccat tgataaaccc agccaatttg aagcggcaat tgattgggac
     7381 atacaatctg gaaaccgccg ccaaaacagc ggctgttttg agacaacgat gttataaaaa
     7441 agcatgtgtg gtgcatagcg aggaaggctt tgacgaagca tcgccgttcg ccggtaatcg
     7501 ggtatttgaa gttgacattg ctcgaccgga tatttacgaa tatttttacc gagcggaaaa
     7561 atgttcccaa aacctgaaca gttatcagct ggtgcaaggc ggggatgccg agaaaaacgc
     7621 gcggataact ttggaaatat taaatggaag gcgaggctat ccgcgtgaaa tgacggcttt
     7681 gaatgcggct tttggtcttt atgtcgccgg taaagtcgac aatattcctg atggaatatt
     7741 ccttgccgaa gaacttatcg acagcggcaa agccttgcag aaactgaatc agtttatcga
     7801 aatgagtagc aaactcgcta attaaacgaa gagatatgaa ttggcttgat actatcgtcg
     7861 ctcataaaaa gcaggaatta atagacgcta agaaagccgt cccgctggaa ttttatcaag
     7921 ctcaaattgg agacaattgc tccaaccata gcttgcttag aacgataaaa cagcaagggg
     7981 gttttcattt catcggcgaa gttaaaaggg cttcaccgtc tcgaggaatt atcaattctg
     8041 atttcgatcc gctccagcag gcgaaatgct atgcgaaagg gggagcaagt gctatttcca
     8101 ttctgacgga ctcgcaattc tttatgggta atttggctga tttgcaagtt attcgagaac
     8161 agattgactt gccgcttctg cgtaaggatt ttatcgttga cccttatcag atttatgaat
     8221 ctcgttatta tcaagccaat attgtcttgc tgattgctcg gattctatcg ccaaagcaaa
     8281 ttacccaatt ttgcgagtta gcaacgaaac tggctctgga agttttattg gaattggcaa
     8341 ccgagaacga tttagtaaaa attccgtcta atcattccaa tttaattctg gcgatcaaca
     8401 accgtgatct ggcaaatttt gaagtggatt tgaataattc cttccgtctg aagccccttt
     8461 tacctgatga tataccggtc atcagtgcca gcggaataag taacaccgac gagtgtgtac
     8521 gactttttaa aaacggcttt gccggtgcat taattggcga atcattaatg cgtgatcgcg
     8581 aaccggatag tttattgaag aagttcagga gtgcaatcgc taatgccggt taaattgaaa
     8641 atctgcggga ttacgtcgct ggaggatgct ttacttgccg ctcaacttgg tgccgattgg
     8701 ctgggattta atttttatcc cgcaagtccg cgctacattg caccggaact cgccgccgaa
     8761 attattaagg tgcttccgag gacggttgag agtgtcggtg tattggtaaa accttttctg
     8821 gaagaagctc gcaaaattat cacaatcacc ggagttcagc gcttgcagat ttacgagccg
     8881 caggatttca atgactattc ccaattagga gtgccggtga tagcttgtta ccgttgtaaa
     8941 aatggtaaaa taaccgaatt aaattatgcg tcggctgata tggttttgct ggataattat
     9001 gcggaaagta tttttggggg aaccggccaa gcctttaatt ggcgttggat tccgaccgaa
     9061 ctggaacgaa agaaactggt gctggcaggc gggatagata ccactaacat cttggaagct
     9121 ttagaaatga ttggtccagc cgtaattgac gtagccagcg gaagtgaatc aaagcccggt
     9181 cacaaagatc cggaaaagat caagacatta gttagtcaaa ttagaatatt taatcagaga
     9241 aaggacaatt aattcaatgg aaaaaggata ttttggcgaa tatggcggac agtatgttcc
     9301 ggaaatcctg cgtccggcat tggtcgaatt ggaagaaata tatttagcgc ttaaagatga
     9361 tcccgatttt caaaaacggt taaacattga gttgcacgat tacgccggtc gtccaacgcc
     9421 tttatatttt gccgaaaacc tgacccgcca ttatggtcag gctaaaattt atttgaaacg
     9481 tgaggatttg aatcataccg gcgctcataa aattaacaat gccattgggc agattcttct
     9541 ggcgcaagca atgcataaga agcgcatcat cgccgagact ggcgccggtc agcatggcgt
     9601 tgccgttgcg accgtggcgg cgcgagccgg tctggaatgt tgcatttata tgggagcgga
     9661 agatattcaa cgtcaatatc ccaatgtgcg acgtatgcaa cttctggggg ctgaagtccg
     9721 tccggttcat actggaacag ctactctgaa agatgctacg aacgaagcct tgcgggattg
     9781 gctggctaat gtaaaaacta ctcattatat aatcggttcg gtagtcggac cacatccgtt
     9841 tcccacgatt gtgcgtgact ttcagaaaat tattggcgag gaaacacgcg aacaaattgt
     9901 cgagaaaacc ggtcgtttgc cggactacat tctggcttgt gttggcggcg ggagtaacgc
     9961 catgggtatt ttttatccgt ttatttctga taccgctgtc aatttaatcg gagtagaagc
    10021 cgctggagaa ggacttgcca gtggcaaaca ttcggcgaca ctgagttgtg gctcactcgg
    10081 tatttttcat ggaatgaaat ccttcgtact tcaaaataat gacggccaga ttcaattgcc
    10141 ctattcgctc tcagccggat tggactatcc gggcgtcggt ccagagcatt gtcatcttaa
    10201 ggatagtggg cgagtcaagt attatgctat aactgatact gaagctttag ccgcggtaga
    10261 gcttttatgc cgactggaag gcatcatacc ggcgcttgaa tccgcgcatg ctctcgctta
    10321 tcttgaatat cttttgccac agactcatag tcgggaatcc gtagtggtga acctgagcgg
    10381 tcgaggcgat aaagatctgg aaacatatct caaatatttg gaaaggcggt aaggaaatcc
    10441 aatgagcacc agattaaatc aaattctaaa aagtaaaaaa gaaaaacagg aaaaatgtct
    10501 ctcgattttt ctcacagccg gttttccgga gaaaaattgt actcctgaga taattttaac
    10561 cctggctgca agcggggtgg actttatcga gttgggaatt ccgttttccg atcctatcgc
    10621 cgacggtcca gttattcaga aagcttccga taaagccnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnctg gatttgattc atttaatagc tccgaatact cccaccgagc
    10801 gcattcggca aattgacgaa atcagtaccg cttttattta ctgtgtggct tacaccggcg
    10861 ttaccggcca ggataaccga ccaactcccg agacaatcgc atttttcaat catttaaagc
    10921 agactctctg gcatccttgg ctgatcggtt ttggcgttaa aagtcatgag gatttccaaa
    10981 cttacactca ctatgccgac ggggtgatta tcggctcagc cttcattaat ttgctggaaa
    11041 atacacccga aaataatcgc cttgccgctt gtcgcacgtt tgtccaaaat ataataaact
    11101 caccatcatg atgtacttga tgaccttagc agaaaattat gctgtatatt tatacataaa
    11161 ttatacaaaa taataagaaa atattcataa atatttaaag tagtccgcca ataatctggg
    11221 taaaatttat tatattatta cgcttttttg gctgaaagga ttatatgaaa attgtaaaaa
    11281 gctacgccga gattaaccag aagataaaaa accgtcaggc agttgtgatg acggcggagg
    11341 agataaagag ctttattcgg gaacagggta ttaaaagtgt tttggagaaa gttgatgtcg
    11401 tcacgactgg tacttttggg gcaatgtgtt cttccggagc atttttgaat tttggtcata
    11461 ccgacccgcc cttaaaaatg gaaaaagtga aaattaacga cgttccagca tacggtggtt
    11521 tagcggcggt ggatgcttat atcggtgcta ccgagcgttc ccaaaaatcg aatggtcatt
    11581 acggcggagg ccatgtcata gaggatatgg tagccggcaa aaatctcgtc ttagaagccg
    11641 aaggcaaacc tactgattgt tatccgcttg attccattgt aactgaattt acgctcaaag
    11701 accttaatca ggctgttctg ctcaatccac gtaatgctta tcaacgttac aatgccgcgg
    11761 ctaattcgac tgatcgtatt atttacacct atatgggaag attactgcca cggctcggta
    11821 atgtcaccta ctccggcgcg ggtgaactgt cgccgctcaa taatgacccg aattttttgg
    11881 cgatcggtct gggaacacgt attttcctag gcggcggagt aggatatgta attgattcgg
    11941 gcagtcagca cagtccggaa acaggtttcg ccaatttaat ggttaagggc gatttgaaac
    12001 ttatgcgtcc cgaatatcta cgcggagctt atgtctatgg ctatggctgt actctgttcg
    12061 ttgggattgg cgtgccgatt ccacttttga atgaagaaat tgctcgttcg gttgccataa
    12121 ccgatgacga actcagggta aatattgtgg attacggaat tccacgattg aaacgtccaa
    12181 tcctgagaac cggagttaca taccgggagc tcaaatccgg tcaagtagaa attgagggca
    12241 agccggttcc tacgggttcg ctttccagtc tgtttatggc tcgcaaaatt gctaatgaat
    12301 tgaaaaattg gattctgaac ggcaattttc tcctgaccga agcgatcgag ccaattccca
    12361 agatcggaaa agccaaacaa ttagccgtca agccacccaa aaaaccacag gcaatatcaa
    12421 caaccaacca gaaacattcc aacggaattc aaattgacca ttctcgttgc atcctttgtg
    12481 aactgtgtat cgcccagtgt ccggagaagg ctatttatct aaatgaaaac aatcaaataa
    12541 ctattgacca agctaaatgc aatctgtgtc gtatctgcac taaagtttgt tcagttggct
    12601 gtctggaata aagtaaggca agagatgaac caaacggtaa ctttccgata taaaaataca
    12661 attgcccgca ttacgggcga gcctgaattg gttaaaattg gtgaggaaga aattatccgt
    12721 tgttatacag acatagaaaa ctatatctcc aacgatccaa tttttaaagc gacgttgaag
    12781 ccctataaag ttccgaacga cgccagtaaa attatacggg aaatggcgac tgccggagct
    12841 atttgcggag tcggtccgat ggcggcagta gccggggcaa ttgctaaata tgcagttctg
    12901 gcgatgcaaa aagccggtgc cacctttgct atcgttgata acggcggtga tatcgcaatt
    12961 ctaaatgacc ggccagtagt ggttgggatc tacagtggac cggctgtaat tcacgatata
    13021 ggcttgcgaa ttccggtgca ttctacgcta accagcgtct gtacttcatc cggggtgatc
    13081 ggacatgcac taagcttcgg gaagtcgcac gccagcgtca ttctcgctga cgatccggtg
    13141 ttagcggacg ccgccgcgac tgctttagga aatcgcatcc gtgaaaaaga taaatttgaa
    13201 attgaaaatg cgatgaatga ggtactgaaa ttcggtgtag ccggatgtat cgtaattatt
    13261 gaagattatg ttggatttgc gggagaaatc ccagaaattt gccgcgttga aatgagtgaa
    13321 agtatgattg ccaaatgaag gaatttgaat cggtatgaaa gtattgaaat tcggtggtgc
    13381 cagtttgaaa gataaaaacg cctatgcgga aacaatccgt attttagcgg aggataaaga
    13441 accgaaggca gtcgttctat cagcaattta tggcatgacc gattggttgg aaactacaat
    13501 ccgggaggct cgcgccaacg agcaatccat cgaaaatacc acaactaaga tcagagcctg
    13561 gcatgctaaa atattaaccg acttagttac cgatccgact atgatatcgg actgtatgaa
    13621 tgaattagaa gagcggattc agaaactcga acgcctgctt tatggtctgg cttataccga
    13681 agaaatgacc cctcagcttt acgctctgat tatgagcttt ggtgaacggc tatcggtaat
    13741 tttgtcggcg acgattttta aggctcatgg cattccggct cagtatttag agtctgataa
    13801 aattggaatt gtcaccgacg attcctttac cagtgctacc gcattgttgc caatagttcg
    13861 taaaaatttg ccatcgcatg tctatccatt gcttgaaaaa ggcattacgc cgctgattac
    13921 cggattcttt ggggttaata aaaacggcaa agttaccctc tttggccgaa atagttccga
    13981 ctatagcgcg gcggtcattg cccaaggttt gaaagccgac cgtttagaaa tctggaagga
    14041 tgtggatgga tttatgaccg ccgatccaaa gattgtgccg acggcgacct taattgataa
    14101 attatcctac tacgaagcgg ctgagctttc ctacttcggt gctaaaatat tacatccgcg
    14161 cacagtcgag ccgttgattc acgactcgat tccattagtg attcgtaata tttatcatcc
    14221 ggagattgaa ggcaccgaaa tttgtgctaa agaaattctc aatacggcaa ttattaaaag
    14281 tgttacttat aataaaaaca tttcggtttt acggataaaa ggcgctggtg ttggttataa
    14341 accgggcgtt atttccgaaa tcggcagtcg cttagccgcc tgtggagtta atatttactc
    14401 gattattacc tcgcaaacct gtattaattt gttgattgac cgcaaagatt ctaaagtcgg
    14461 ctataaagct ctccaagttt tgaaagacgg attgattgat tcgatcgagc attgcgatga
    14521 tgaagtcgtc gtagcggcag ttggcgcggg attgaaacag acacccggtt tagccgcgaa
    14581 aattttcagt gctattgctg gccaaaatat taacatcgaa atgatttcat tgggcgcttc
    14641 ggatgtagcg acctatttta tcgtcaaaga gccttgttta gtgccggcag tcaatgcttt
    14701 gcatacagct ttttttacca attctattaa ttaaaggaaa aaaattgaaa tcgaacagtc
    14761 agttaaagtc caaaatccca gttggtattc tgggtgccac cggtagcgtc ggacaaaaat
    14821 tcatcgaact attgacagaa cacccctggt ttgaaattgc cgctttagcg gcttcagatc
    14881 gctcggctgg caaaacttat cgcgatgccg ctcattggtt tatggctaag cctttaccag
    14941 acaacgttgc gaagatgata gtaaaaaatt gtgagccaaa tctaccctgt cgcattgttt
    15001 tttccgggct ggatgcttcc gtggcaggag aaattgaaac tgattttgct aaggccgggt
    15061 atattgtaat ttctaattca cgcaatcatc gctatgaccc cgatgtgccg attctggtac
    15121 cggaagtcaa ttccgatcac ttggcaatta tcgctactca gaaatatgga cgaggtgcga
    15181 ttatcaccaa tccgaattgc gccgcgaccg gtttggttgt ggctctcaaa cctttactcg
    15241 atcaatttgg gctttcggcc gttaatattg ttactatgca agcgatttcc ggtgctggtt
    15301 atccgggcgt cgccagtctg gatattgtgg ataatgttat cccatttatc agcggtgaag
    15361 aagcgaaatt agaaagtgaa ccccaaaaga tactcgggaa ataccttgat gggcatatcg
    15421 aaccagccca atttatcatt agcgcggctt gcaatcgcgt cgccgtcgtt gatggtcata
    15481 cggaatgcgt ttcagttaaa ttggaacgaa aaccgactgc tggagagatc atcgccgcct
    15541 ggcgtgaatt caagggtgaa ccgcaacgtt tgaaactgcc aagcgcaccg gaaaagccgc
    15601 tttgctattt tgaagaaagc agttatccgc aaccgagatt acatcgcctt ctggaaaacg
    15661 gtatgaccgt tagtctcggt cgtctgcggg agtgtcccat cctcgattat aagtttgtga
    15721 tgttatcgca taatacggtt cggggtgcgg ctggtggagc cattctcaat gccgaacttt
    15781 ttattgccaa aggcgggttg gaaaaactta ggta
//
LOCUS       DAOOMU010000049        15735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_3949_length_15735_cov_16.774426, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000049 DAOOMU010000000
VERSION     DAOOMU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15735
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3949_length_15735_cov_16.774426"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PLI55_06075"
     CDS             complement(<1..1088)
                     /locus_tag="PLI55_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPB00437.1"
                     /translation="MKIRTFIIFLTIVLLAYFIVNYYIFIHGLKAFGANQTAKLIYTI
                     LFVILVSAYFLGRIIERISIGVVPQALEWLGAYWFGAMVYFLLIVIIIDIFGVIYHFI
                     PLIPNDLRQYLQQLNLITGIVTVTVVAIILVIGRWNATHPQIKTVGIDIHKKAKAETM
                     TIAFASDIHLGSFVGQRQLRQLVELINANQPDVILFGGDLLDEDLAPVIRHNLGGCLA
                     ELKAPLGKFTVPGNHEFIGGIEKATQYLENHGIKVLRDEALQLANGVWIIGRDDYDSK
                     RFSSDVQRLTLKYLIDKINPEQPIIVLDHQPLNLSESIASRIDLQLSGHTHNGQLWPL
                     NYLTAKVYQVSHGYKKIDGTHFIVSSGFG"
     gene            complement(1131..1979)
                     /locus_tag="PLI55_06080"
     CDS             complement(1131..1979)
                     /locus_tag="PLI55_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB00438.1"
                     /translation="MNCLKTIIISLVLFFNVINVLGDEPAATVIPDWSLKQVFKVPES
                     ALYDPERDVIYVSNINGSPSAADGNGFISRVSPNGKILDLHWIEGLNAPKGMAIYRDT
                     MYVADLNAIARIDLKQGRLIGKIDLKGKFLNDITVDENGTIYCSDNVADIIYIVKNGQ
                     PAIWLSGGNYNPNGLLAEKNQLVMLSSSNGTLNYIDYKNKSVRKVATIGGSLDGVVAC
                     DNNYLVSSFPGEIYLVSPNGQSKKILDTKTNKISSADIGYIPQKRLLLVPTFSDNSLM
                     TYKIVR"
     gene            2143..2886
                     /locus_tag="PLI55_06085"
     CDS             2143..2886
                     /locus_tag="PLI55_06085"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPB00439.1"
                     /translation="MNKTELIRTDEYIIHSYEVDCERRLRLSVLNRYMQESAWQHAET
                     LGAGYASLLSKNFAWVLTRTVISIEELPIWGNSLKLSTWPSGLDHLFAYRDFLFLNTD
                     NQEIGRATTSWCVIDLETRRPQPVAEIFPFQLPQDKGLLFPERPPKVESLTGADYIWQ
                     TRVGYQDIDTNEHVNNGRYIDWIYETFPLEFLKTHHPQRLEINYLAEAFYNHEIIVTT
                     QKTSEIIYLHSVVKMPDHTELCRAKTIWR"
     gene            3008..>3417
                     /locus_tag="PLI55_06090"
     CDS             3008..>3417
                     /locus_tag="PLI55_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPB00440.1"
                     /translation="MAIHPNILTTIGKTPLVRINKLTEGTSAEILAKIEFFNPGASVK
                     DRIALNMVEDAEQRGILKKGMTIIEPTSGNTGIGLAMVAAVKGYQVVFVMPETMSIER
                     RKILKQFGAQIILTPGNQGMSGAVAEAERLAQQDG"
     assembly_gap    3418..3517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3518..3706
                     /locus_tag="PLI55_06095"
     CDS             <3518..3706
                     /locus_tag="PLI55_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPB00441.1"
                     /translation="DESAILTARRLATEEGILVGISAGANLFAALQIARRNRHDCLII
                     VVFPDTGERYLSTELFEE"
     gene            3708..4295
                     /gene="cysE"
                     /locus_tag="PLI55_06100"
     CDS             3708..4295
                     /gene="cysE"
                     /locus_tag="PLI55_06100"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPB00442.1"
                     /translation="MNFYFIEDLRAIWRNDPAAKNMLEILTCYTTLHAIMLYRIAHFL
                     HTYLHIPILPRFISVLARFWSGVEIHPGAKIGKGFFIDHGTGVVIGETAEIGDNCVLF
                     HGVTLGGTGHHKGKRHPTIGNNVLIGAGATLLGPIFVGDNAKIGATTVIINRDVPPNC
                     TVVGTPGHIVKRNGKCVNEPLPLARYKHNDTKCNP"
     gene            4613..6442
                     /locus_tag="PLI55_06105"
     CDS             4613..6442
                     /locus_tag="PLI55_06105"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB00443.1"
                     /translation="MALMQKMRNHTHVILWALLILFVASLTVGGLVGGADLLDVFSQK
                     SRLKDAAGIVDGEKLDAVRFSELIQNEINNYRDQNQELTESDIEQISDYIWNSYVNET
                     LINKQIDRYKLYATDNEIYEVLVNNPPQFLIQNEAFQTNGQFDYQKYLNALNNPQGNE
                     WLAVEEYVRIYLPFEKMQYLIESLAMVSDAEVIEEIVLNQTKANFEALVIPYTLVARD
                     TFAIDKSEIKKYYDANRKNFYVPETRSLDFVVFETKPTPADTFSALQTIESIRERLAN
                     GEDFATLASEFTEDPSGVANGGDLGWFGKGQMVPEFEQAAFALRKGEVSQPVITSFGV
                     HLIKVEDKRVENGRPEVKARHILIKIKTSPETLENIRAQANLFAFDANEYGFQAAADS
                     HKLVVRNTGPFNKDAKYINYFGPFPAAIRWAYSNIPVGTVSEVLTHEMGITVMCLKEI
                     NKEHYRPLSEVENQIKNILTSEKRIEKLKSISEEIYALVTQDNKFDNAIAKYPQCLLD
                     SYTDHPVNAPLKNVQQSNAITGTLLALQPGQISKPVLVANRNLVIIKLHSRQDVTPEI
                     IVQEKEATRMRILNNKKSAFYTAWINDLKANAKIVDNRSNLYY"
     gene            complement(6522..7391)
                     /locus_tag="PLI55_06110"
     CDS             complement(6522..7391)
                     /locus_tag="PLI55_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00444.1"
                     /translation="MSKKSISIIAAIVILVVLLLVNWKNHQTRLAPTSDNGEWFFLQK
                     SDLRKSPHHTKHARIFGKYVRGYNYYILKGIDKVQAHAPDGGEYFTTLKSRQLESPIG
                     YELKIFGKSLIGPPRRSSYSSGATYGAFIEAMNIMYGKGGHDSLDFEHYEALRMQEIG
                     GGKRREGVKFWGYWNSHGFGNHFALVQYSGIGKAVEPRSARPGDFVNIIWKKDGATSA
                     IFLGWFKDKDEKEKIVYWSSQKETNGFGDQIMPVDNIKHLKFVRVTNPEKLFDFNIDM
                     PVNFKVAGDKVVF"
     gene            complement(7402..7770)
                     /locus_tag="PLI55_06115"
     CDS             complement(7402..7770)
                     /locus_tag="PLI55_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00445.1"
                     /translation="MIWFYFCVAVLLVLSGTIIFFKAAWFKSLLQFVIQKDLLLIPGV
                     FEIAIGLGTLYFRDLTHMTWFVYLAGLLMFLDGILSVLMSRRLREMYEWFINIEDRKM
                     RSYAIFLFLVALGYFLAAIR"
     gene            complement(7774..8973)
                     /locus_tag="PLI55_06120"
     CDS             complement(7774..8973)
                     /locus_tag="PLI55_06120"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPB00446.1"
                     /translation="MTQNVSQPQPLVKSQVALVRCDSYEPARVAAAIRRGIDLLGGIE
                     KFAQSGERIVLKPNLLSAHPPEENVTTHPTVIKAIAEIMLENGVKVFIGDSATRGHLA
                     KILRESGMQTVIDELNLPIADFENGKQVYSPDSVRNKKFTIARGVWESDGIFNVPKLK
                     THHLTRITAAIKNLFGCVPGFLKPALHVALPAVDDFCRMLVDLSIFLKPRLTILDAIE
                     AMEGNGPSSGNAYPLKLLAFSSDLVAIDSVVCRMMAIKPEYVRTNYWGAQAGLGKINL
                     EEIEILGDGLDTFSAPDFKVQRQPDLRFNQAWQYRHLKGLVTNRPVIDAQKCKKCGDC
                     IDQCPQQPKALSWNGKMKTQPPQFDYKLCIRCFCCQEVCPHKAIDSYTPLLRRIVDKV
                     YTNIYGA"
     gene            complement(8978..9694)
                     /gene="rsmI"
                     /locus_tag="PLI55_06125"
     CDS             complement(8978..9694)
                     /gene="rsmI"
                     /locus_tag="PLI55_06125"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016197395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HPB00447.1"
                     /translation="MKSGILYLVATPIGNLADITFRAIETLREVDLIAAEDTRHAAIL
                     LHHYEIRKPVVSYYEENEKRRSQELLDKLSAGQNIALITDAGTPLLSDPGFQLVRRAI
                     ENDIRIVPIPGASSILTALMASGLPTDRFTFEGFLPRKKGRLTRLNELANEPRTMVFF
                     ESPHRVLETLQDFLEKFGDRPAVCAREMTKVYEEFRRDRLSNLIKHFENHPAKGEFVI
                     IVEGIKRKSKNHFGSQPQTH"
     gene            complement(9810..10652)
                     /locus_tag="PLI55_06130"
     CDS             complement(9810..10652)
                     /locus_tag="PLI55_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPB00448.1"
                     /translation="MAKKTSVFKIVLFLICVVIILIYGFNRLKDRLPRKLINVIAPTK
                     ITVMTYDINHGMGNDNVYNLSRISKVIKNESPHIVILNEVDYKTRRGLNDEQARRIAA
                     ELGMEFTFSRNYPVDGGWTGNAILSKFPIEFAENKIYKQDYGHNTKSLLHIIVNIGNI
                     KVHVYGTQLSSDSIASSNQVKELLNFVFEWGNDSPVIIGGNFEMTPDYKRIYEMAYYF
                     TDVGAYFQTEAFTYPASEPYKRLDYLFSNKLAIPLKVKVSDNEITRVASDHLPVVAHF
                     KIIY"
     gene            10818..11348
                     /locus_tag="PLI55_06135"
     CDS             10818..11348
                     /locus_tag="PLI55_06135"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPB00449.1"
                     /translation="MPAKPVKYFIGALFSDKNLLAESLRRAENIFDTADLVSADFPFN
                     LTDYYTSEMGSPIFRRFVSFSKLFSPDFLASAKIHTNSIEDDLRLDGLRKVNLDIGYV
                     DYDKVVLASAKYGIHKIYLGQGIYADLTLHYVKGHYQPYDWAFQDFELPDYHSFFLKM
                     REIYKQQIKQMLLACQ"
     gene            complement(11368..12252)
                     /locus_tag="PLI55_06140"
     CDS             complement(11368..12252)
                     /locus_tag="PLI55_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPB00450.1"
                     /translation="MNHNRNHNQNTDNNIGLAFGLNLGFAIFELFGGLWVNSIAVISD
                     ALHDFGDSLSLGVAWYFKRLSRRQKNNRFTYGYQRYALLGAIITMIVLVVGSVIILWE
                     AFPRLLHPTIPNSTGMFVFAIVGIVVNGFAVLRLKGYSSLTEKAITWHLMEDVLGWIA
                     VLIVSVILKFTDWYFLDPLISILITIFILYKVIGNFRATMAVFLQSAPANIDNDKIVA
                     HLSQLEKVKSAHHTHIWSLDGEHHVLTTHIVVEADTSKDEVLMIKNRIKELAEKYNFE
                     HLTVEIEYEGEDCSMNCD"
     gene            12472..13722
                     /gene="pepT"
                     /locus_tag="PLI55_06145"
     CDS             12472..13722
                     /gene="pepT"
                     /locus_tag="PLI55_06145"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HPB00451.1"
                     /translation="MDKEILEHFFRYVKIDTQSQEDVTDRYPSTAKQLDLAKILVKEL
                     IDLGLKDAAMDEFGIVMATLPSNLAPTEAARIPVIGLIAHLDTSPEVSGKDVKPIIHQ
                     NYRGGDIKLPGDPNVIIRQSENPILAEKIGEDIVTSDGTTLLGADDKAGIAEIMSFLS
                     YLQKHPDIKHGTLRIAFTPDEEVGAGTDHFDVKKFGADFAYTVDGETVGEIEIETFNA
                     SAGIFTVHGINVHPGYAKGKMVNAIPIAAEIVRLIDREPSPATTEKREGYLHPYVIEG
                     GVEKVTLRVLLRDFDADGIRAKTVRLNEIRDQVASQFPLARVELEVKESYKNMYVKLA
                     ENPKVIEYALEATRRAGIQPKTQIIRGGTDGAKLCFMGLPTPNIFTGGHNFHSKQEWI
                     SVQDMEKTVATLVNLVQVWVEKSL"
     gene            13764..15458
                     /locus_tag="PLI55_06150"
     CDS             13764..15458
                     /locus_tag="PLI55_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014562006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HPB00452.1"
                     /translation="MFQSIKKSIPLWAIINLICLGIFMTLSNVTCGDKVDNSKADLAY
                     VKAELNKLAPVELKCDLSSLSVADRQVLVRLVEAGHIIDQLFLIQVNPENPKILTKLQ
                     TSTDENDLPYLELFTIMFGPWNRIDHNKPFIGKKEKPLGAGFYPEDMTREEFEKFIAE
                     HPEQKKAFESEFTIIKRQKGQLVAIPYHQEYQPYINAISNLLIEAAEITQDPTLKKYL
                     NQRANDLKTDDYFQSDMDWMDLAGDLEIVIGPYEVYEDNLFNYKAAYEAFICMVDHAE
                     SEKLAKVTKYLDEIEANLPLAKEYRNYSRGKVSPIKVVQEIFSSGDTKAGVQTTAFNL
                     PNDERVRSAKGSKKVMLKNVAEAKYEKCWVPIVNEILAPEPLKNVSFDAYFTHVLMHE
                     VCHGLGPGILKNPDGSETTVRNELKECYSTIEECKADVLGIFSYLYLIDKGVFAQDSK
                     YTALATYLGGMFRSIRFGINEAHGGCVAIQFNYFIDKGAFLTDAEGKLTIAPAKLETA
                     IKDLAAELLLIEAKGDYEGAKAIIEKYRIATPLITETVKKLEHLPIDIRPIYPITF"
BASE COUNT         4748 a         3405 c         3045 g         4437 t
ORIGIN      
        1 ccaaaaccgc ttgatactat aaaatgggtg ccatcaattt ttttataacc atgactaact
       61 tgataaactt ttgcggtaag gtaatttaat ggccacaatt gtccattatg ggtatgtcca
      121 gataattgca aatctattcg actcgctata ctttcggata aatttaacgg ttgatgatca
      181 agaacaatga tcggctgttc aggattaatt ttgtcaatca agtatttcaa tgtaagtcgc
      241 tgaacatcac tactaaagcg tttgctatcg taatcgtccc gaccgattat ccaaacgcca
      301 ttagccaact gcaacgcttc atcacgtaag acctttatcc cgtgattttc aagatattgg
      361 gtggcttttt caatcccgcc gataaattcg tgattgccgg gaacggtaaa tttccccagc
      421 ggcgctttga gttctgcgag acagccgccg agattgtggc gaattactgg cgccaagtct
      481 tcatccagca gatctcctcc aaataaaata acatccggtt ggttggcatt aattaattct
      541 actaattgac gtaattgacg ctggcctaca aatgaaccaa gatgaatatc actagcaaat
      601 gcaattgtca ttgtttcagc tttggctttt ttatgtatat caattcccac agttttgatt
      661 tgcgggtgag ttgcattcca gcgtccaatt accaaaataa tcgcaaccac ggtaacagtg
      721 acaatgccgg taattaaatt caactgctga agatattgcc gtaagtcatt cggaatcagc
      781 ggaataaagt gataaatgac accgaagata tcgattatta taacaattag taaaaaataa
      841 accatagcgc cgaaccagta agctccgagc cattccaacg cttgcggtac aactcctatg
      901 gaaatcctct cgataatccg gccgagaaaa taggctgaaa ccagaataac gaataaaatc
      961 gtataaatca actttgccgt ttggtttgcc ccaaaggctt taagtccgtg tataaatatg
     1021 tagtaattga caataaaata agctaataga acaatcgtga ggaatatgat aaacgtccga
     1081 attttcacgt aaaatataaa cctaattctt tattattaaa cggattaaat ctaacgaaca
     1141 attttatacg tcatcaggct attatctgag aaagtgggta ctaatagtaa acgtttctgg
     1201 ggaatgtagc caatatcagc cgacgagatt ttattagttt tagtatccag gattttttta
     1261 ctctggccgt tcggacttac caaataaatc tctcccggga aactagatac aaggtaatta
     1321 ttatcacagg ctactacacc atccagagag ccgccaatcg tagctacttt acgaacagat
     1381 ttgtttttat aatcaatgta atttaatgtg ccattgctgg aagaaagcat cacaagttgg
     1441 tttttctcag ccagtaaacc gttcggattg tagttgccgc cggagagcca gattgctggt
     1501 tgtccatttt tcacgatata aataatatca gcgacattgt ccgaacaata gattgtaccg
     1561 ttctcgtcaa cggtaatgtc gtttagaaat ttgcctttca agtcaatttt accgatgagt
     1621 cgcccctgct ttagatcaat ccgtgcaatt gcgttcaaat cagcgacata cattgtatct
     1681 cgataaatcg ccataccttt cggggcattc aaaccttcaa tccagtgcaa atccagaatt
     1741 ttaccattcg gcgatactcg cgaaatgaaa ccgttaccgt ctgccgccga cggactgccg
     1801 ttaatattac tgacataaat gacatctcgt tcaggatcgt acagcgctga ttccggtact
     1861 ttaaaaacct gtttcaaact ccagtctgga ataacggtag cagccggttc gtcacccaag
     1921 acgttaatta cattgaaaaa aagcaccaaa gaaatgataa ttgttttcaa acaattcata
     1981 atctatcctc catcttcttc attgatttaa tcttaaaaat ccttgagcaa caaatcaaca
     2041 attttttaat gaggtaattg cagaaaatta acaggtacaa atggtttgtc ggaatttatt
     2101 ttcactgaaa gtttgttata ttatctatcg tttgaaaaaa ttatgaataa gaccgaacta
     2161 atcagaactg acgagtatat tattcattcc tacgaagttg attgcgagcg acgcctgcga
     2221 ctttcggtgc tgaatcgtta tatgcaggaa tcagcctggc aacatgcgga gacgctgggc
     2281 gctggctatg ccagtctact gtctaaaaat ttcgcctggg tgcttactcg aactgtcatt
     2341 agcatcgaag aattgccaat ttggggcaat tcattgaaac tttcgacctg gccttccgga
     2401 ttggatcatc tctttgccta tcgtgacttc ttatttctga acactgacaa tcaggaaatc
     2461 ggtcgagcaa cgacttcgtg gtgtgtgata gatttagaaa ctcgtcgccc tcaaccagtg
     2521 gctgagattt ttccttttca attgccgcaa gataaggggt tacttttccc agaacgtcca
     2581 ccaaaagtcg agtcgctcac cggagcggat tatatctggc agacccgagt cggctatcag
     2641 gacattgata ctaacgaaca tgtcaataac gggcgctaca ttgactggat ttatgagact
     2701 tttcctctcg aatttcttaa aactcatcac cctcaacgtc tggaaatcaa ctatttagct
     2761 gaagcttttt acaatcacga aattattgtt acaacccaga aaacgtcgga aataatctac
     2821 ctccattcgg ttgttaaaat gcccgaccat accgaacttt gccgcgccaa aacgatttgg
     2881 cggtagattg aattaaaata tatgccgatg cttcagacaa attttactgg aatttttaat
     2941 tctccctgaa aatttctttg gatttaaatt attagagcct taaagtttac gaaaattagg
     3001 aaaagtgatg gctatacatc caaatatatt gacaactatt ggtaaaactc ctctggtacg
     3061 aattaataaa ttgacagaag ggacatcggc tgaaatcttg gctaagattg aatttttcaa
     3121 tccgggcgct tcagtcaaag atcgtattgc tctgaatatg gtcgaagatg ccgaacagcg
     3181 cggaatttta aaaaaaggga tgacaatcat cgaaccaact tccggtaaca ccggaatagg
     3241 cttggcgatg gtcgccgcag tcaaaggata tcaggtcgta tttgttatgc cggaaacgat
     3301 gagcatcgag cgtcgcaaaa tattgaagca atttggagca cagattatcc taacacctgg
     3361 caatcaagga atgtcgggcg cagtcgccga agccgaacgt cttgcgcaac aagatggnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngat gaatcggcta ttttgactgc
     3541 ccgccgtttg gcaaccgaag aaggtattct ggtgggtatt tcagcgggcg ccaatctgtt
     3601 cgccgctctt caaattgccc ggcgtaatcg gcacgattgt ctgattattg tggttttccc
     3661 agacacgggc gaacgttatt tgagcacaga attgtttgag gaatagaatg aatttttatt
     3721 ttatcgaaga ccttcgcgcc atttggcgca acgatccagc cgccaaaaat atgcttgaaa
     3781 ttttaacttg ctatactact ctacacgcaa ttatgctcta tcggattgcg cattttttgc
     3841 atacctattt acacattccc attctgccac gcttcatcag tgtattagct cgcttctgga
     3901 gcggagttga aattcatccc ggcgcaaaaa tcgggaaagg attcttcatt gatcatggta
     3961 caggagttgt catcggcgaa accgccgaaa tcggtgataa ttgcgttttg tttcatggtg
     4021 ttactctcgg cggcactggt catcacaaag gcaaacgcca tcctacaatc ggtaataacg
     4081 tccttatcgg cgccggtgca actcttctcg ggccgatttt tgtgggagac aatgccaaaa
     4141 ttggcgccac gaccgttata attaaccgtg atgttccacc taactgcaca gttgtcggta
     4201 ctcctggtca tattgtcaag cgcaacggca agtgcgttaa tgaaccactt ccgctggcac
     4261 gttataaaca caatgatacg aagtgcaatc cataaaatat gaagaaaagc ggagggattt
     4321 tctctctgaa accgacaatt tctatttcgg tgttaccgag gtaggcatca ggattgttca
     4381 tttcataaat acttactaaa aatccccagg acatccccaa aaacttagga tagagacggg
     4441 ataactagct aaataacagc ataattcata gacttaaaaa gccttatcta ttttcctaaa
     4501 aatttgagtg aagtcctgct tatataactt tttgtcaatc ccggcaattt tacttatatt
     4561 tagcgtcaat ttttgaagtg gttaatataa aattcggagg tcttgataaa ctatggctct
     4621 aatgcagaaa atgcggaatc acacccatgt aattctctgg gcgcttttaa tcctatttgt
     4681 agccagtttg acagttggtg ggctagttgg aggtgccgat ttactggatg tcttttccca
     4741 gaaatcccgt ctcaaagatg ccgctggaat tgtcgatggc gagaaattgg acgcggtgcg
     4801 cttttcggaa ttaattcaaa atgaaatcaa taattaccgt gaccaaaatc aagaattgac
     4861 agaatccgat attgaacaga tctccgatta tatctggaat tcatacgtca acgagacgtt
     4921 gattaataaa cagatcgatc gctacaaact ctacgcgacc gataatgaga tttatgaagt
     4981 tctggttaat aatccacctc agtttttgat acaaaatgaa gcgtttcaaa ctaatggcca
     5041 attcgattac cagaaatatc taaatgctct taacaacccg cagggcaatg aatggttagc
     5101 tgttgaggaa tatgttcgta tttacttgcc tttcgaaaaa atgcagtatt tgatcgaaag
     5161 tttagcgatg gtttcagatg ccgaagtcat tgaagaaatc gtgttaaatc agactaaagc
     5221 caatttcgaa gcgctggtaa taccttacac tttggtcgct cgggatactt ttgcaattga
     5281 taaatcagaa ataaaaaaat attatgatgc caaccgcaag aatttttacg ttccagaaac
     5341 tcgctctttg gattttgtag tctttgagac caagcccact ccggcggata ctttctccgc
     5401 tttacagaca atcgaatcta ttcgggaacg cttggctaac ggcgaagatt ttgctacttt
     5461 ggcttcagaa ttcaccgaag acccgagcgg tgttgccaac ggtggcgatc tgggttggtt
     5521 cggcaaaggt cagatggtac ccgaattcga gcaagccgct tttgctttac gtaaaggtga
     5581 agtatcccag ccagttatca catctttcgg agttcatttg attaaagtcg aagataaaag
     5641 agtcgagaac ggccgaccag aagtcaaagc ccgtcatatt ttaatcaaaa tcaaaactag
     5701 cccggaaacc ttagagaata ttcgcgccca ggctaatcta tttgccttcg atgccaatga
     5761 atacggtttc caagccgcgg ctgattcaca taaattagtt gttcggaata ctggaccatt
     5821 taacaaggat gccaaatata taaattactt cggtcctttt cccgccgcca tcagatgggc
     5881 ttattccaat ataccagtcg gcactgtatc ggaagtcctg actcatgaaa tgggcatcac
     5941 ggttatgtgc cttaaagaaa tcaataaaga acactatcgt cctttgtcag aggttgaaaa
     6001 tcaaattaaa aatatcctaa cctcggaaaa aagaatcgag aaattaaaat ctatcagcga
     6061 agaaatttat gctttagtaa ctcaggataa taaatttgac aatgccatcg ccaaatatcc
     6121 tcagtgcctg ctcgattcct ataccgatca tcccgtcaat gcaccactga aaaacgtcca
     6181 gcagtccaat gctattaccg gtacgttatt agctttacag cccgggcaaa tatctaaacc
     6241 ggtgttggtc gctaatcgta atctggtaat catcaaactc cattcccgtc aggacgttac
     6301 tccggaaatt atcgtccagg aaaaagaagc cacccggatg cgaattttaa acaataaaaa
     6361 gtcggcattt tacacggcat ggatcaatga tttaaaagcc aacgccaaaa tcgttgataa
     6421 tcgcagtaat ctgtattatt aaaaattcaa ttatatcata aaagtaaaag cccggcgatc
     6481 taccgggctt ttttattcaa aatattaaaa caatttctcc atcaaaatac gactttatca
     6541 cccgccactt tgaaattgac cggcatatca atattaaaat caaataattt ttccggattg
     6601 gtcactcgta cgaatttaag gtgcttgata ttgtcaaccg gcattatttg atcgccgaag
     6661 ccgttggttt ccttttgact tgaccaatag acgatctttt ccttctcgtc tttatccttg
     6721 aaccatccga gaaaaatggc tgatgttgca ccgtcttttt tccaaataat attgacgaaa
     6781 tcgcctggtc tggcactccg aggttcaact gcttttccaa taccgctata ttgaaccagc
     6841 gcaaaatgat taccgaaacc gtgcgagttc caataacccc aaaatttgac gccttctctt
     6901 cttttgccgc cgcctatttc ctgcatacgg agagcctcat aatgttcaaa atcaaggcta
     6961 tcatgtccac ctttcccata cataatattc atcgcctcga taaaagctcc gtaagtagcg
     7021 ccgctactat aactgctccg acgaggcggc ccaatcagcg acttaccgaa aatttttagc
     7081 tcatagccga ttggtgattc aagctgtcga gattttaaag tagtaaaata ttctccccca
     7141 tcaggtgcat gcgcctggac tttatcaata cctttcagaa tatagtaatt gtacccgcgc
     7201 acatattttc cgaaaatccg ggcatgttta gtatgatgcg ggcttttacg caaatccgat
     7261 ttttgtaaaa aaaaccattc accattatca gaggtcggag cgagtcgggt ctgatgattt
     7321 ttccaattga cgagtagcag aactaccaga attacaatcg ccgcaatgat ggaaattgat
     7381 ttcttactca aattttactc atcatctaat cgccgctaaa aaatagccaa gcgccactaa
     7441 aaataggaaa atggcatacg aacgcatttt gcgatcttca atgttgataa accattcata
     7501 catttcacgc aaacgtcttg acattaacac cgatagaata ccatccagaa acataagcaa
     7561 gccagctaga tatacaaacc aggtcatatg agtcaagtcc ctgaaataaa gcgttccgag
     7621 accgatagca atctcaaaaa cacccggaat caataatagg tctttctgga ttacgaattg
     7681 aagcagggat ttgaaccagg cggctttaaa gaaaataata gtcccagata gaaccagcag
     7741 aactgccaca caaaaataaa accagatcat tattcaagct ccataaatat ttgtatatac
     7801 tttatccaca attcgtcgta aaagcggtgt ataactgtca atggctttat gcggacaaac
     7861 ttcctgacag caaaaacagc gaatgcacaa tttataatca aattggggcg gttgcgtttt
     7921 catttttcca ttccaggaca aggctttagg ttgttgcgga cattgatcaa tacaatcacc
     7981 acacttttta catttttggg catctatcac tggtcggttg gtcactaacc ccttcaagtg
     8041 gcgatattgc caggcttgat taaagcgcaa atccggttga cgttgaacct taaaatccgg
     8101 agctgagaaa gtatccagac catcaccgag aatttcaatt tcttccagat tgatcttgcc
     8161 caacccggct tgagcacccc aataattggt tcggacgtat tcaggtttaa ttgccatcat
     8221 ccggcacact accgaatcaa ttgccaccaa atcactcgaa aacgccagca gtttaagtgg
     8281 atacgcattt ccgctactcg gtccattgcc ttccattgcc tcaatagcat ccaaaatcgt
     8341 aagacgcggt ttcagaaaaa tgctcaaatc aaccagcatt cggcagaaat catccaccgc
     8401 tggtaacgcc acatgcagag ccggtttgag aaaacccgga acacagccga agaggttttt
     8461 gatagccgcc gtaatacgag tcaaatgatg agtctttagt ttgggaacat taaatattcc
     8521 atcggattcc cacactccgc gagcaatagt aaatttttta ttacgcacgc tgtcaggcga
     8581 ataaacctgc ttgccatttt caaaatcggc aattggcaaa tttaattcgt caataacggt
     8641 ttgcatcccg ctttcccgta aaattttcgc caagtgtccc cgcgtcgccg aatcaccaat
     8701 aaaaaccttt actccgtttt ctaacataat ctcggctatc gctttaataa cggtgggatg
     8761 agtcgtgaca ttttcctccg gaggatgagc actgagcaga ttcggtttga gcacaattct
     8821 ttcgccggac tgggcaaatt tttcgatacc acccagcaaa tctatcccac gacgaatagc
     8881 cgccgccacc ctcgctggtt cataactgtc acatcttacc agagccactt gagacttcac
     8941 taacggttga ggttgtgaaa cattctgcgt cataatttca atgggtttgt ggttgcgaac
     9001 cgaagtggtt tttagattta cgtttgatgc cctcaacaat aattacaaat tcgcctttcg
     9061 ccggatgatt ttcgaaatgt tttattagat tgctcaggcg gtcgcgacga aattcctcgt
     9121 aaactttggt catctcacga gcacagaccg ccggtcggtc accgaatttt tccagaaaat
     9181 cctgaagcgt ttccagaaca cgatggggac tctcaaaaaa taccattgta cgtggttcat
     9241 ttgccaactc gtttaagcgc gtcaaccgcc cttttttacg tggtaaaaaa ccctcaaatg
     9301 taaaccgatc ggtgggtaag ccggacgcca tcaaagccgt tagtattgac gacgctcccg
     9361 ggatcggaac aatccggatg tcgttttcta ttgctcttct taccagttga aaacccggat
     9421 cgctcaatag aggcgttccg gcatcagtga tcaacgcaat attttgaccg gcgcttaatt
     9481 tatcgagtaa ttcctgactg cggcgttttt cattttcttc ataatagctg actaccggtt
     9541 tccgaatctc ataatgatgc aataagatag cggcgtggcg agtatcttca gccgcaatta
     9601 aatcaacttc ccgcagagtc tcgatcgctc gaaaagtgat atcagccaaa ttgccaatcg
     9661 gtgtagccac cagatataaa atgccgcttt tcatatttcc gttatgttat tcagattctc
     9721 ttaatatttt aattaattct acaatttttt aggttataaa ttcgcccgat tatgccattt
     9781 cgaataaaag gaaaatgcct tgttaattat tagtaaatga ttttaaaatg cgccaccacc
     9841 ggcaaatgat cggaagccac ccgcgtaatt tcattgtccg acacttttac ctttaacggt
     9901 atcgccagtt tattcgaaaa aagataatcc aggcgcttgt aaggttccga agccggatag
     9961 gtaaatgcct ccgtttggaa atatgccccg acatccgtga agtaataagc catttcgtaa
    10021 atacgcttat agtccggcgt catttcaaaa ttaccaccaa ttatgaccgg actatcgttt
    10081 ccccattcaa aaacaaagtt cagcagttct ttgacctgat tcgagctggc aatcgaatca
    10141 ctacttaact gcgttccata tacgtgaacc ttaatatttc cgatattaac aatgatatga
    10201 agaagacttt tggtattatg gccataatcc tgtttgtaaa ttttattttc agcaaattca
    10261 atcggaaatt ttgataaaat agcattgccg gtccagccgc catcaacggg ataattgcga
    10321 gaaaaggtga actccattcc gagctcagcg gcgattcgtc gggcttgttc atcatttaaa
    10381 ccacgtctgg ttttgtaatc cacctcattg aggatgacta tatgcggcga ttcatttttg
    10441 atgaccttgg aaatgcgcga tagattatag acattatcat ttcccatacc gtggttaatg
    10501 tcatacgtca ttacagtaat tttagtcggc gcgatgacat taattaattt acgtggtaaa
    10561 cgatccttaa gccggttaaa tccataaatt aggataatta caacgcagat taagaaaaga
    10621 acaattttaa agacagatgt tttttttgcc atataaagtt ggttaagttt aaaataataa
    10681 aattcaaaaa actgattgga attttacgaa tttttcttta attggcaatt gctttatcgt
    10741 ttttggggtg ttttaggaat aactccctga tttgaaaatt tcaaaattga taatagatta
    10801 actcataaat gaagccaatg cctgctaaac cggtcaagta tttcatcggc gccctttttt
    10861 ccgataaaaa cctcctagct gaatcgctcc gccgagccga aaatattttt gacaccgccg
    10921 atttagtcag cgctgatttc cccttcaatc ttacggacta ttacacatca gaaatgggta
    10981 gtccgatttt tcgtcggttc gtttcattta gtaaattatt tagtcccgat ttcctggcat
    11041 cggctaaaat tcacaccaac tctatcgaag acgatttgcg cctcgatgga ctccgtaagg
    11101 tcaacttaga cattggttat gttgattacg ataaagtcgt attggcttca gctaagtatg
    11161 gaattcacaa aatttatctc gggcaaggta tctatgctga cttgacttta cattatgtca
    11221 aaggtcacta tcagccttac gactgggctt ttcaggattt tgaattgccg gactaccact
    11281 cattttttct gaaaatgcgc gaaatttaca aacagcaaat taagcaaatg cttctagctt
    11341 gtcagtaata gtcaattata ttatcactca gtcacagttc attgaacaat cttcaccttc
    11401 gtattctatc tcgacagtaa gatgctcaaa attatatttt tcagccagtt ccttgatgcg
    11461 atttttaatc ataagcactt cgtctttact ggtatctgcc tcgactacaa tatgcgtcgt
    11521 caagacgtga tgttcgccgt ccagcgacca gatatgagta tgatgagcag atttgacttt
    11581 ctctaactgt gagaggtgcg caacaatttt atcattatca atattggctg gcgccgattg
    11641 cagaaaaaca gccatagtcg ctcgaaaatt gccaattact ttatacagaa taaaaattgt
    11701 aatgagaatc gaaatgagcg gatccagaaa ataccaatcg gtaaatttca atatgacact
    11761 cacgattaaa accgcaatcc agcctaacac atcttccatc aaatgccagg taatcgcttt
    11821 ctcagtcaac gacgaatagc cttttagacg tagaacagca aatccgttga caactattcc
    11881 aactatggca aaaacgaaca taccagtcga attaggtatg gtgggatgca aaaggcgtgg
    11941 aaaagcctcc cataaaatta tcaccgagcc gaccaccaga actatcatag taataatcgc
    12001 tcctagaaga gcataacgtt gataaccgta agtgaaacga ttattcttct ggcggcgcga
    12061 aaggcgcttg aagtaccagg caacgcctag tgacaggcta tcgccaaaat catgcaacgc
    12121 gtcggaaatt accgcgatac tattcaccca cagtccacca aacaattcaa aaatcgcaaa
    12181 tccgagattc aaaccaaaag ccagtccgat gttattgtcg gtattttggt tatgattacg
    12241 attatggttc atattataaa ttccttttta agggaatcac aggctcacga ttggtggttt
    12301 cattacggat gtaggagttt ttgtttttat ataatgttgt gaagtattga taaaacacat
    12361 ttgtttctca tcaacagaat ttctttccaa aagcactttt taatatacaa attgttatca
    12421 ataattacta ttttatagcc agtgaaatta ggagacagaa aggaataatt catggataaa
    12481 gagatactcg aacacttttt tcgttacgtg aaaatcgaca cccaatccca ggaagatgtc
    12541 actgaccgct acccgagtac ggcaaaacaa ctcgatttag ctaaaatttt agtgaaagag
    12601 ttgattgacc tcggattaaa agatgccgct atggatgaat tcggaatcgt tatggcaact
    12661 ttgccttcca atttagcgcc gactgaagcc gccaggattc ccgttattgg gctgatcgct
    12721 catctcgata cttcaccgga agtcagcggt aaggacgtta aaccgattat tcatcaaaat
    12781 tatcgaggtg gagatattaa attaccaggt gatcctaacg tcatcattcg tcaatccgag
    12841 aaccctatct tagccgaaaa aatcggcgag gacattgtca cttccgatgg tacaactcta
    12901 ttgggcgccg atgataaagc cgggattgcc gaaataatgt cttttttgtc ttacttgcaa
    12961 aagcatccag acatcaaaca cggcactttg cggatcgctt tcactccgga tgaagaagtc
    13021 ggtgccggta ctgaccattt cgatgttaag aaattcgggg ctgactttgc ttataccgtt
    13081 gacggtgaaa cagtcggaga aatcgaaatt gaaaccttca acgcaagcgc cggaattttc
    13141 acggttcatg gtatcaatgt tcatcccggt tacgccaaag gcaaaatggt caatgccatt
    13201 ccgattgcgg ctgaaattgt tagattaatt gatcgtgaac cgagtccggc aactactgaa
    13261 aaacgcgagg gctatctcca tccttatgtc attgaaggcg gcgttgaaaa agtcacccta
    13321 cgcgttttat tacgcgactt cgatgccgat ggcattcgcg ccaaaaccgt ccgtttgaac
    13381 gaaattcgcg atcaagtcgc ctcccaattt ccactggcgc gtgttgaact ggaagtcaag
    13441 gaatcctata aaaatatgta cgtaaaacta gccgagaatc ccaaagtcat cgaatatgct
    13501 ctggaagcta cccgacgtgc cggaattcag cccaaaactc agattatccg aggtggcact
    13561 gacggcgcca agctctgttt tatgggtctt ccgactccca atatttttac cggcggtcat
    13621 aattttcata gcaaacagga atggatttcc gtgcaagata tggaaaagac cgttgcaact
    13681 ttggtgaatc tcgtccaggt ttgggtggaa aagtcgctgt aatttatttt attgaacatg
    13741 aaataataca aaatggagga aaaatgtttc aatctattaa gaaatcaatc ccattatggg
    13801 caattatcaa cctgatctgt ctgggaattt ttatgacttt aagcaatgta acgtgtggtg
    13861 ataaagtgga taatagcaaa gccgatctgg cttatgtaaa agctgaactt aataaacttg
    13921 cgcctgtgga actgaaatgt gacctatcct cactctcggt tgccgatcgg caagttctgg
    13981 ttcgcctggt cgaagccgga cacatcattg accagttgtt tctcatccag gtcaatcctg
    14041 aaaatccgaa aattcttacc aaattacaaa cttcgaccga tgagaacgat ttgccttatc
    14101 ttgaactatt cacgattatg tttgggcctt ggaaccgaat cgatcacaat aaaccgttta
    14161 tcggcaaaaa ggaaaaaccg ctcggcgccg gtttttatcc ggaagatatg accagggaag
    14221 aattcgagaa attcatcgcc gaacatcctg aacagaaaaa ggcttttgaa agtgaattta
    14281 ccatcatcaa gcgccaaaaa ggtcaactgg tcgccattcc ttatcatcaa gaataccaac
    14341 catatatcaa tgcaatttct aatttgttga tagaagccgc cgaaatcact caagacccga
    14401 ctttaaaaaa gtatctcaac caacgggcaa atgatcttaa aaccgatgat tatttccaga
    14461 gcgatatgga ttggatggac ctggccggcg acctggaaat cgtcatcgga ccgtatgaag
    14521 tgtacgagga taatcttttc aattacaaag ccgcttacga agccttcatt tgtatggttg
    14581 accacgccga aagcgaaaaa ttagccaaag taaccaaata tctcgatgaa atcgaagcca
    14641 acctgccact cgccaaagaa tatcgcaatt acagtcgcgg taaagtatcg cctattaaag
    14701 tcgtacagga aattttttca tctggtgaca ccaaagctgg agttcaaaca acggctttta
    14761 atttgccaaa cgacgagcgg gtgcgttccg ccaaaggctc caagaaagta atgttaaaaa
    14821 atgtagccga agctaaatac gaaaaatgct gggttccgat tgtcaatgaa attttagcgc
    14881 ccgaaccact taaaaacgtc tcattcgacg cctattttac gcacgtgctt atgcacgaag
    14941 tctgccatgg tctcgggccg ggaatcctga aaaatcccga cggcagtgaa accaccgtgc
    15001 gcaatgaatt aaaagaatgt tactccacta ttgaggaatg caaagccgat gtgctgggca
    15061 ttttttcgta tttgtattta attgataaag gcgttttcgc tcaagactca aaatacacgg
    15121 ctctggcaac ttatctcggc ggaatgtttc gttcaatacg gttcggcatc aatgaagccc
    15181 atggcggatg tgtagctatc caattcaatt actttattga taaaggtgca ttcttgaccg
    15241 atgcagaagg caagctgact atcgcccctg ctaaactcga aaccgcaatt aaagaccttg
    15301 cggcggaatt acttttaatc gaagccaaag gcgattacga aggcgccaaa gccattatcg
    15361 aaaaatatcg tattgcaact ccgctaatca ccgaaactgt caagaaattg gagcatctac
    15421 cgatcgacat tcgtccgatt tatccgataa cgttctaact atttaaatta aataagccac
    15481 tcaacggaag gtcaaaattc aatcttagat tcgatttaaa ctcaatcgat agttagatac
    15541 tttaagaaaa gatcaatata aaaaattagt ccaaaaaata actaataggt caagctgtct
    15601 ttaagatatt gcgttttcta cctaaaatta gtgcgcgttt gctgacattg acttgttgaa
    15661 tttgcccagc aaagcttggt atacttcagc cagtttggtt ttatcaacta aaaatatcaa
    15721 aactgaagtc ataat
//
LOCUS       DAOOMU010000050        15409 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4083_length_15409_cov_25.348118, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000050 DAOOMU010000000
VERSION     DAOOMU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15409
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4083_length_15409_cov_25.348118"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1605
                     /locus_tag="PLI55_06155"
     CDS             244..1605
                     /locus_tag="PLI55_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPB00453.1"
                     /translation="MKQHKFTLSESEMPKAWYNIAADLPYALPPALHPGTQKPITPDD
                     LAPIFPMSLIEQEVSTERFIPIPEPVLEIYQLWRPTPLVRAYRLEKAIGTHSKIYFKN
                     ESVSPPGSHKPNTAVAQAYYNYKEGVKRLTTETGAGQWGSALAFACNYFGLECVVFMV
                     RISYETKPYRKSMIETWGGRIFASPSQETASGRAILANDPNCAGSLGMAISEAVEVAM
                     NDKNTKYSLGSVLNHVMLHQTIIGEETQKQIAQTGDYPDIIIGCVGGGSNFAGFSFPF
                     IRDKIAGKKLRAIAVEPTACPSLTKGLYSYDFGDTAKLTPLLAMYTLGHNFMPSKIHA
                     GGLRYHGASPLVSSMVKEGLIEARSYQQTEVFKAAVTFARSEGIIPAPETAHAIKATI
                     DEAKKADEEGVTKTIMFNFSGHGHFDMSAYDSYFAGRLEDNHFSDASLQKNLAELADL
                     PKI"
     gene            1719..2483
                     /locus_tag="PLI55_06160"
     CDS             1719..2483
                     /locus_tag="PLI55_06160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPB00454.1"
                     /translation="MKVLYSKPQVKTIQIDGLGSVTLRRDHRARRLTLKVKPDQQITL
                     TVPYSISEKTALAFLEANREWALKNIQALKEIKPPIFTIEDKQTIQTRTHRLALQPTS
                     NNEIRVGIQPGIINVQYPSNQKITNPRIQEAIKLGLTAAYRIEAKQFLPPRVRDLAQR
                     YGFRYNRLFIKNLKSRWGSCSAANNINLNLHLMRLPDELIDYVILHELTHTQIKNHSP
                     LFWQELERILPDAKVRRQQLKTITVQGFLPSVVMEN"
     gene            2588..4513
                     /locus_tag="PLI55_06165"
     CDS             2588..4513
                     /locus_tag="PLI55_06165"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010884405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HPB00455.1"
                     /translation="MYKSQKTSLKSEDQDSKSDANPFPDCLDTADWRKLTALQNQYVE
                     AIVTEYIRLCRPAKVSILSDTPEEIAYVRQLALRTGEEQPLKIAGHTVHFDGYNDQGR
                     DKEHTRILVNDGHLNSERLNTLDRVKGLEEIHHLLDGIMAGKEMLVRFYCLGPLGSIF
                     SIPALQITDSSYVAHSEDLLYRQGYSEFRRLNGSDRFFHFIHSAGELDERGCSKNIDK
                     RRIYIDLESNRVFTINNQYAGNSVGLKKLALRLAINKANHEDWLAEHMFIMGVHPLQK
                     KRTTYFTGAFPSACGKTSTAMLPGQTIVGDDIAYLRVSNDKKCNAVNIESGIFGIIAD
                     VNPIDDALIYRCLTTPRELIFSNVLVKDGKPYWLGMGCETPESGINHSGEWFKGKKDA
                     AGKEIPLAHKNARYTMRLSELENLDPRADDPNGVPISAIIYGGRDSDTNPPILQALDW
                     LHGVMLGATLESETTAATLGQEGQRTFSPMANLDFVVVPLGLYIENHLKFGARLAKAP
                     LVFSTNYFLKDSSGNYLNEKTDKKVWILWAEGRVHNEYDAIATPVGYLPQYNDLQNLF
                     KLALGKDYSRTAYEQQFTLRIDKFIAKLDRVSEFYRNETVPAEFNNRLDQQRADLVKL
                     QEEYQAPEISPFQIVKR"
     gene            4623..4835
                     /gene="rpmE"
                     /locus_tag="PLI55_06170"
     CDS             4623..4835
                     /gene="rpmE"
                     /locus_tag="PLI55_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPB00456.1"
                     /translation="MKPNIHPEYKECTVTCACGNTFVTRSTVGDLKVEICSACHPFFT
                     GKQKLIDSAGRIEKFNRKYKRDTQKG"
     gene            4896..>5564
                     /locus_tag="PLI55_06175"
     CDS             4896..>5564
                     /locus_tag="PLI55_06175"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HPB00457.1"
                     /translation="MAQKILVGGQAVIEGVMMRVPGAYATAVRRPDGEIEIKRENFTS
                     IADRIPFFKKPLLRGIISMFESLKIGLETIQLSANIAMQAENAAKPANKTKDRIATTI
                     SMILAFGLAFLLFGFLPLWLTTQLLNIERKALAFNLVVGAWRILFFLMYLWLISFMKD
                     IQRLFQYHGAEHKVVFTFESGQELTVENAQPFKTYHPRCGTSFIFIVMMVGILLFALI
                     DAVVI"
     assembly_gap    5565..5664
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5665..6279
                     /locus_tag="PLI55_06180"
     CDS             <5665..6279
                     /locus_tag="PLI55_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPB00458.1"
                     /translation="GTQNGLGGFKEVIFLLSGENVYGTMKFESGVHRVQRVPVTETQG
                     RIHTSAATVAVLPEAEEVDIEINASELRIDTYRSSGAGGQHVNKVETAIRITHLPTGL
                     VVTCQDEKSQYKNKEKALKILRSRLLALKEAEKEAEIAAKRRSMVSTGDRSAKIRTYN
                     FPQGRVTDHRINLTLYRLTEVMEGEIDELISALQLAHNTELMKS"
     gene            6387..7268
                     /gene="prmC"
                     /locus_tag="PLI55_06185"
     CDS             6387..7268
                     /gene="prmC"
                     /locus_tag="PLI55_06185"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPB00459.1"
                     /translation="MAERERQIWRIVDVLKWSRDYLKGKQIESPQIEAEWILREVLNL
                     NRLEIYLKYERPLSPAELARIKALLLKRASGQPIQYVLGSAEFMGLKLKVTPDVLIPR
                     PETELLVEKTLELCRNRVTARILDIGTGSGCIAIALAHFLPECQVVAIDISATALQIA
                     RANAAIHNVANRIDFQELDILHSPELSMGQFDIIVSNPPYVSGDYYVNLPKLVKEHEP
                     EIALNPGTDGLLFYRRIKELASEIAIPGGAVVLEIGGDYQAAAVAELFSESAFDSVAV
                     VKDYSGQSRVIIAELAK"
     gene            7265..8104
                     /locus_tag="PLI55_06190"
     CDS             7265..8104
                     /locus_tag="PLI55_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPB00460.1"
                     /translation="MKKLLAYFKIMRPLNLLQGGIAILVCMALADPKPEWLSVLLALV
                     IVWAYTGAGNILNDYFDVEIDRINRPQRPIPAGLIQPRTALFYAIFLFALGTAAVVPV
                     FNRQLAIIIPFALFFLITYTKFFKPRPLWGNLIVSAILGMTFLFGSSIFGDIRVGIVP
                     CLLAFGFNFVREIVKDMQDVAGDQARGARTFPLVYGLTAARRLVIVATLILMVGALVP
                     FYLKIYGKFYLLILVLTVEIPLIYVIFSISRDASATNCGRIAAILKGDIFFGLLAIFA
                     GKF"
     gene            8105..11536
                     /locus_tag="PLI55_06195"
     CDS             8105..11536
                     /locus_tag="PLI55_06195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB00461.1"
                     /translation="MTQFVHLHNHSDYSLLDGAQSIESLVNRAVELGMPAVGLTEHGN
                     LFSAIHFLKAANTSGLKPILGCEIYVADNNRFDKKSREQGGSGYHHLLLLVQNRQGYE
                     NLIKLVSLGYLEGYYYRPRVDNELLRKYNAGLIATSACIKGRIPDLAIKGDYEKAKQL
                     ALEYAEIFAGRFYLEVQNHGIDDELRWYEIAKKLSAEIGLPRLLTNDTHYTLPEHWEA
                     HDAHLCIGTGKEMSDPDRMKYEQHEYWVKSAEEMAALYPNDPEIIENTLKIAEQCDFN
                     LEMNQYYLPEFPIAESAKVKTADEYLAQLIEERLPGRYPEITPEIQERVDYELGVIRQ
                     MGFAGYFLIVQDFVHYAKNNNIPVGPGRGSAAGSIVAYILGITNIDPLKYKLIFERFL
                     NPERISMPDIDIDFCDTKRGAVIDYIRKKYGENCVAQIITFGKMKARAVIRDVGRVLS
                     MPLAEVNRIAKLVPEQINITLETAINNTPELQEAVNIDEQHQKLFAISKVLEGLNRHA
                     STHAAGVVIAPGNLTDYVPLYQSSNGEVTTQYDMKCIDQIGLLKVDLLGIRNLSVIDN
                     TLAMLKAKGIELDIDKIPEKDEETLALFGEGRTIGIFQFESSGMREYLKKLKPSGIED
                     LIAMNALYRPGPMEMINDFIDRKQGRKPITYLHPLLEPILVDTYGIIVYQEQVMQIAH
                     QVAGFSLAKADLMRKAMGKKQKKTMESLKKEFVEGAIKNGIKRSVAVQIYELIQRFAE
                     YGFNRSHSAAYAILAYQCGYLKAHYPAEFMAANLSSEINDTDRITILANEARNMGYEI
                     LPPDVNYSEVRFIPEGKAIRYGLHAIKNVGEKAAENIVATRQTEKQFRTLFQMVAALD
                     LKSVNRKVLESLIAAGAMDSLEGTRGQKFAAIDTAIQYATHIQEEKNNNQVNLFGIAN
                     GAPQATLSEPRLPDVAPWEDSEKWNKEKELLGMYLSGHPLLEYSTEIESFSNYDFTEP
                     LSNLDKSNVKLGGLIASIKTMTDRKGRPMAFINLESLNGKVELIAFADIYEKYREHLK
                     INYPIFVSGNVNCRNEDDAKIIVEEISPLRGLLNRKSKKLHVRINVNQFGTEDLENLK
                     KLLAKHTGECQLFLHLYDGNGVNKSIRSGNLRVSADRELLDILKKTFGEDNAWVE"
     gene            11536..11982
                     /gene="dtd"
                     /locus_tag="PLI55_06200"
     CDS             11536..11982
                     /gene="dtd"
                     /locus_tag="PLI55_06200"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPB00462.1"
                     /translation="MIAVIQRVSQAHVTVENKIVGRIGPGLVILLGVFQNDGDADAAF
                     LADKIAGLRIFGDEQQKMNLSIKDVGGAALVISQFTLCGDWRKGRRPSFISAADPETG
                     RRLYEKFIADLKIAGVPTEQGIFGAMMAVSLVNDGPVTFVLDSKLR"
     gene            11987..12700
                     /gene="radC"
                     /locus_tag="PLI55_06205"
     CDS             11987..12700
                     /gene="radC"
                     /locus_tag="PLI55_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPB00463.1"
                     /translation="MRSKEPAVYRLPITAWPESDRPREKLMKAGPMKLTEAELLAILL
                     RSGAGGNSALDVAREILRKSGGLNALAQMDYHDILNLKIRGIGPTKAVTIAAGLQLAR
                     RLQAVSVSPPEMIIHKSDEVAKIYIPRLQDLKKEIFMVVLLASNNKIIRDAIISEGIL
                     NASVVTPREVFREAILGMAAAIILIHNHPSGNPEPSNEDLQLTRQMKEVGQAMNIPVL
                     DHIIIAGAQYTSFADKELL"
     gene            12921..14186
                     /locus_tag="PLI55_06210"
     CDS             12921..14186
                     /locus_tag="PLI55_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00464.1"
                     /translation="MEIIERATLQQHITNTPDSYLRPLLAALYLEDQDCQSAIELCRQ
                     DLAIHPFSTIGHYIWALAALAMNDLNQAAIHLRAIIQIDGKFLQAYYKLTELGGDYLT
                     QAELKYCYEKICDLNPYDKKSADRLSAFPLELQSQKPQSPAKPERTDQVKESSEKAIM
                     AEPIESAPEIDPLTGANRLSQFFESVIKTTVTPNSSEIKPTPVPEEEAHFGPIEKTAI
                     ETEPTVEPEPTISFDEPFPEEPQVKESSGSSSISDLFSRLREKPFEEVQKENWLKEHL
                     SGETPSPAMPTITESDKPAVEPQAVSQPQSETKATDIPAKSKATATPKVAPTKAGKAS
                     QKPKSASNKQRQSPQPKKSAASPGNISFPIPTWTLVDILMKQKLYEQALNILDIIESK
                     SKNEKDLEKVQASRAKIVRLMAEEQSGEA"
     gene            15119..15358
                     /locus_tag="PLI55_06215"
     CDS             15119..15358
                     /locus_tag="PLI55_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00465.1"
                     /translation="MDNYESAITALKIPFFPLGLRFPAGNVLNAVMVNWMVNFSCRGC
                     LLFSLSPYLSLNLFTQPLYSASLLMTNDQCPMTIH"
BASE COUNT         4345 a         3388 c         3424 g         4152 t
ORIGIN      
        1 aattataaaa aatacttgca ttttttccaa aacatccgta aatattcgcc cgtatgaata
       61 gaattagtgc attaaaaaat cggcaaaata ccctgaactg gtggtggcaa attagtaatg
      121 gggcggtgaa atgctgaaat aagcgtttaa gcaactctat attggcatag acccgcccga
      181 aaaaaggcgg gtttttttgt tttattgaca ataacgaaaa aataacaaag aaaggaaatg
      241 aaaatgaagc aacacaaatt caccttgtct gaatccgaaa tgccaaaagc ctggtataac
      301 attgcggctg acctgcccta tgctctacca ccggctttac atcctggtac ccaaaaacct
      361 attacgcccg atgacttggc acctatcttt ccaatgtctc ttatcgaaca ggaagtgagt
      421 acggagcgtt ttattccgat accggagccg gtactcgaga tttatcagct atggaggcca
      481 acaccgttgg tgcgggctta tcggttggaa aaagccatcg gcacacactc caaaatttat
      541 ttcaaaaatg agagcgtgag cccgcccggc agtcacaaac ccaataccgc agttgcgcag
      601 gcttactaca attacaaaga gggagtcaaa cgcttgacca ccgaaactgg cgccggacaa
      661 tggggtagcg cgctggcttt tgcctgcaat tactttggtc tggaatgcgt cgtatttatg
      721 gtgcgcatca gttacgaaac caaaccatac cgtaaatcta tgatcgagac ctggggcggt
      781 cgtattttcg ccagtccgag tcaagaaact gccagcggac gagcgattct cgctaatgat
      841 cctaattgcg ccggtagtct gggaatggca atttccgaag cggttgaagt tgctatgaat
      901 gataaaaata ccaagtactc gctcggctca gtactaaatc acgttatgct ccatcaaact
      961 attatcggag aagaaactca gaagcaaata gcccagaccg gtgattaccc tgatattatt
     1021 atcggttgcg tcggcggcgg cagtaatttt gccggttttt cttttccgtt tatccgggat
     1081 aaaattgccg ggaaaaaatt gcgagctatc gcggtcgaac cgactgcctg tccgtcgttg
     1141 accaagggtc tatattctta tgattttggc gatacggcta aattgacgcc attgttagcg
     1201 atgtacacgc tcggtcataa tttcatgccc tcaaaaatcc acgccggtgg gctacgctat
     1261 catggcgctt caccattggt tagctcgatg gtaaaagaag gcttgataga agcgcgctcc
     1321 tatcaacaaa ctgaagtgtt caaagccgcg gttacttttg cccggagcga aggtatcatc
     1381 cccgcaccgg aaaccgctca tgccatcaaa gccactattg atgaagccaa gaaggctgat
     1441 gaggaaggtg ttaccaagac gattatgttc aacttcagtg gtcatggtca cttcgatatg
     1501 agcgcttacg attcctactt cgccgggcga ttggaagaca accatttttc agatgcctca
     1561 ctgcaaaaga acctagctga gcttgctgat ttgcccaaaa tctaagagta aaattactag
     1621 gttatcgaac ggggcgggat aaatcccgcc ccgcttgtct gtcactgaaa ctctgggctg
     1681 gtttgaacaa ttaaagtttt ttacattcta ctcttacgat gaaagtctta tattcaaaac
     1741 ctcaagtgaa aactatccag attgatggcc ttggttcagt taccctgcgt cgcgaccacc
     1801 gcgcccgacg actaactctg aaagttaaac cggatcagca gattacgctt actgttccgt
     1861 attcaatcag tgaaaaaact gccctcgcct tcctagaagc caaccgcgaa tgggctttaa
     1921 aaaatattca ggctctgaag gaaattaaac cacctatttt caccatagaa gacaaacaaa
     1981 ccatccaaac tcgtactcac cgtttggcat tgcaaccgac tagtaataat gaaattagag
     2041 ttggcatcca gcctggaatt attaatgttc agtatccatc caatcaaaag attaccaatc
     2101 cacgtattca agaggcgatt aagttggggc tgacagccgc ttatcgcatc gaggctaaac
     2161 aatttttacc tccaagagtg cgcgatttag cccagcggta cggttttcgt tacaaccgac
     2221 tttttattaa aaacctcaag tcacgctggg gcagttgctc ggccgccaat aatattaacc
     2281 tcaacctgca ccttatgcgt ttgccggatg agttgattga ttatgtgatt ctacatgaat
     2341 tgacccatac ccaaatcaag aatcatagtc cgcttttctg gcaggaactt gagcggattc
     2401 taccggatgc taaagtacga cgtcagcaat tgaaaacaat cacagtccag ggatttttac
     2461 cgtcggttgt aatggagaat taatttttca agaaataatt atttaaaaac aatgatatta
     2521 gccatcatat taaaaaatac taaaattatt gttgttaatt tcaaaaagga ttttataatt
     2581 tctaattatg tataaatctc aaaaaacatc tctcaaaagc gaagatcaag atagtaaatc
     2641 tgatgctaat ccatttcctg attgtctcga cactgccgac tggcggaaac tgactgctct
     2701 ccagaaccaa tatgtcgaag caattgttac cgagtatatc cgactttgcc gaccagctaa
     2761 ggttagtatc ctcagtgaca ctcctgagga gattgcctat gtgcgacaat tggcgctccg
     2821 taccggcgaa gaacagccgc ttaaaattgc cgggcatacc gttcattttg acggttataa
     2881 tgatcaaggc cgtgacaagg aacatactcg cattttagtt aatgatgggc atttgaacag
     2941 cgaacgcctt aatactctcg atcgggtcaa gggcttagaa gaaattcatc atttgttaga
     3001 cggtattatg gctggtaaag aaatgctggt acgcttttat tgtctcggtc cgctcgggtc
     3061 aatcttttcc attccggctt tacaaattac cgactcatct tatgtcgctc atagtgaaga
     3121 tctactatat cggcagggtt atagcgaatt ccgacgacta aacggctctg atcggttttt
     3181 tcattttatt cacagtgcgg gagaactcga tgagcgcggc tgttcgaaaa atattgataa
     3241 gcgccggatt tacattgatt tggaaagtaa ccgcgtattt acgattaata atcaatatgc
     3301 cggtaatagc gtgggtttaa aaaagttggc tctccgtctg gcaatcaata aagccaatca
     3361 cgaagattgg ctggctgaac atatgttcat tatgggagtt catcctctac aaaaaaaacg
     3421 caccacctat tttactggtg catttccgag cgcttgcggc aagaccagca ctgccatgtt
     3481 gcctggtcag acgatcgtcg gagacgatat agcttatttg cgtgtttcaa atgataaaaa
     3541 atgcaatgct gttaatatcg aatccggtat ttttggcatt attgccgatg tcaatcctat
     3601 tgatgatgct ttgatttacc gttgtttgac aacgccacgg gagctgattt tttcaaatgt
     3661 actggtaaaa gacggaaaac cttactggct gggaatgggt tgcgagacgc cggaaagcgg
     3721 tatcaatcat tccggggaat ggttcaaagg caaaaaagat gcggctggga aagaaattcc
     3781 gctggctcat aaaaatgcgc gttatactat gcgtctcagc gagctggaaa atctcgatcc
     3841 gcgcgccgat gatccgaacg gtgtgcccat ttcagcgatc atttatggcg gacgcgattc
     3901 agataccaat ccgccgattc ttcaggcgct tgattggctt catggcgtta tgctgggtgc
     3961 gactctggaa tcggaaacca ccgccgctac tctgggacaa gaaggacagc gcaccttctc
     4021 tccaatggct aatttggatt tcgttgtcgt gccgctcgga ctttacattg agaatcattt
     4081 gaaattcgga gcgcgcttag cgaaagcgcc acttgttttt tcaaccaatt atttcttaaa
     4141 agatagttct ggtaattatt tgaacgaaaa gactgataaa aaagtctgga ttttatgggc
     4201 tgaaggtcgc gtgcataacg aatatgacgc tatcgcgacg ccagtcggct atctgcccca
     4261 atataatgac ctacaaaact tatttaaact ggcactcggc aaagactaca gtcgaacggc
     4321 ttatgaacag cagttcactt tacgtattga taaattcatt gccaaactgg atcgggtcag
     4381 cgaattttat cggaatgaaa ccgttccggc tgaattcaac aatcggctgg atcagcaacg
     4441 ggctgatctg gtcaaattgc aggaagagta tcaagcgcca gaaatcagtc cattccaaat
     4501 tgtaaaaaga taaaaaatca attaatgagc acctagcaaa aatgccttgc aattaaaagg
     4561 caaggcggtt taaatttgct tgctttttta gcgttaacga atgaaatcaa aggaattaaa
     4621 aaatgaaacc caatattcac ccagaatata aagaatgcac cgttacttgt gcttgtggta
     4681 atactttcgt gacacgctcg actgtcggcg atctcaaagt agaaatttgc tccgcctgcc
     4741 acccgttttt taccggcaag cagaaattga ttgacagtgc cgggcgcatc gaaaaattta
     4801 atcgtaaata caagcgcgat acccaaaagg gttagcttct tttaacaaga ttaacgactt
     4861 cttcacagca gtgaagaggt cgtttttatt tgtatatggc acaaaaaata ttagtcggcg
     4921 ggcaagcagt gattgagggc gtgatgatgc gcgtgccggg agcttatgcc acggcggtgc
     4981 gccgaccgga cggcgagatc gagattaaac gagaaaattt tacatccatt gccgaccgca
     5041 ttcctttttt taaaaaaccg ctcctgcggg gcataattag tatgtttgaa tcgctgaaaa
     5101 ttgggctgga aacaattcag ctctctgcta atattgctat gcaggcggaa aatgccgcca
     5161 aaccggcgaa taaaactaag gatagaattg ctactacgat ttcaatgatt ttagcctttg
     5221 gattagcctt tttactgttt ggatttttac cgctgtggct gacaacccag ttgttgaata
     5281 ttgagcgcaa ggcactggct tttaaccttg tcgttggagc ctggcgcatc ctttttttcc
     5341 taatgtatct ctggctgatt tcatttatga aagatattca gcggctgttt caataccacg
     5401 gggctgaaca caaggtcgtt tttacctttg aaagcgggca agagctaaca gtcgaaaatg
     5461 ctcagccatt taaaacttat catccgcggt gcggcacgag ttttatcttc atcgtaatga
     5521 tggttggcat tttgcttttt gctttgatag atgcggtggt aatcnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnggcact cagaacggtt tgggcggctt taaagaggta
     5701 atttttttat tgtccggcga aaatgtttat gggacgatga aatttgaatc gggtgtgcat
     5761 cgtgtccagc gcgtaccggt taccgaaacc caaggacgta ttcacacctc agcggctacg
     5821 gtggcggttc tgccggaggc tgaggaagtt gatattgaaa ttaatgccag cgaactgcgc
     5881 atcgacactt atcgttccag cggcgctggc ggtcagcacg tgaacaaagt cgagaccgct
     5941 attcgaataa ctcacctgcc gactggcttg gtagtcacgt gtcaagacga aaaatcgcag
     6001 tataagaata aagagaaagc cttgaaaatt ttgcgaagcc gtttactggc gcttaaagaa
     6061 gctgaaaagg aagccgaaat cgccgccaaa cggcgcagta tggtcagtac cggcgatcgc
     6121 agtgccaaaa ttcgcactta taattttccg caagggcgcg ttaccgatca ccgcatcaat
     6181 ttgacccttt atcgcttgac ggaagtaatg gaaggtgaaa ttgatgaatt gatctccgct
     6241 ctgcaattgg ctcacaatac cgagcttatg aagagttaac caccacgaaa agttttccgc
     6301 gctgacgatt cgctaaaaac attttctatt ttaccccaaa atctgcttgc tcggctccaa
     6361 aacaatctta aatttccata aatactatgg cagaaagaga acggcaaatt tggcgaatag
     6421 tagatgtgct aaagtggtcg agagactacc tgaaaggtaa gcagatcgaa tcaccccaga
     6481 tcgaagccga atggattttg cgagaagttc tcaaccttaa ccgtctggaa atatatctga
     6541 aatatgagcg cccgctttct ccggccgagt tagcccgtat caaggcgctc cttttgaagc
     6601 gggctagcgg gcaacctatt cagtatgtac tcggaagtgc cgaatttatg ggcttgaaac
     6661 taaaggtgac gccggatgtt ctgattcccc gaccggaaac cgaactgttg gttgaaaaaa
     6721 ccctcgaatt gtgtcgtaat cgcgtgacgg cgcgcattct cgatattggt accggatcgg
     6781 ggtgcattgc gattgcctta gcccattttt taccggaatg tcaggttgta gctattgata
     6841 taagtgcgac ggcgttgcaa atcgcccgcg caaatgccgc catccataat gtggctaatc
     6901 gcattgattt tcaagaactc gatattcttc actcacctga gttatcgatg gggcagttcg
     6961 atatcattgt ttctaatccg ccttatgtct ccggggatta ctatgtaaat ctgccgaaat
     7021 tagtcaagga gcacgagcca gaaattgccc tgaatcccgg aacagacggc ttgctttttt
     7081 accggcgcat caaggagtta gcgtctgaaa ttgcgattcc cggtggggca gtcgtgctgg
     7141 agatcggcgg agattatcag gcggcggctg tggctgaact tttttccgag agtgcctttg
     7201 attcggtcgc agtcgtcaaa gattatagcg ggcaaagtcg ggtaatcata gcggagctgg
     7261 cgaaatgaaa aaactgcttg cctattttaa aattatgcgt cctttgaatt tactacaggg
     7321 aggaattgcc attttggtct gtatggcatt ggctgatcca aaaccggaat ggctgagtgt
     7381 cttgctggct ctggtcattg tctgggctta taccggagcc ggcaacatcc tgaacgacta
     7441 ttttgacgtc gaaattgatc gcatcaaccg cccccagcga ccgattccgg ctggcttaat
     7501 tcagccacgc accgcattat tttatgctat ctttttattc gccttgggaa cggccgccgt
     7561 agttccggtt ttcaatcgcc agttagcaat catcatccct ttcgccttat tttttctaat
     7621 aacctacact aaatttttca aaccgcgtcc gttatggggg aatctgattg tatcggcgat
     7681 tctcggaatg acgtttttat tcggttcatc catttttggt gatattcggg tagggattgt
     7741 tccttgtttg ctggcttttg gttttaattt tgtgcgtgaa attgttaagg atatgcagga
     7801 cgttgctggt gatcaagctc ggggggcgcg gacttttcct ctggtttacg gattaaccgc
     7861 cgcccgtcgt ctggtaattg tggctacgct gattcttatg gttggcgcgt tagttccttt
     7921 ttatctgaaa atatacggaa agttttattt gctgattctg gttttgacgg ttgaaatccc
     7981 gttgatttat gttatatttt caattagtcg cgatgcttcc gcgaccaatt gcggccgtat
     8041 agcggcgatt ttgaaaggtg atatattttt tggcctgctg gctatttttg cagggaaatt
     8101 ttaaatgaca caattcgttc atctccacaa tcattcggat tatagtctat tggacggagc
     8161 ccaatctatc gaatctctgg taaatcgggc agtcgagctg ggtatgccag ccgttggctt
     8221 gaccgaacac ggcaacctct tcagcgcgat tcatttcctt aaagccgcca atacaagtgg
     8281 cttgaagccg atcctcggat gtgagattta tgtggcggat aataaccgtt ttgataaaaa
     8341 atcgcgcgaa caaggtggta gcggctatca tcatctacta ctattagttc agaatcgcca
     8401 gggatatgag aacctgatta agttagtcag tcttggctac ctggagggat attattatcg
     8461 gccaagagtg gacaatgaac ttctgcgaaa gtataatgcc ggactgattg ctaccagcgc
     8521 ctgtatcaaa gggcgcattc cggatttagc catcaaaggc gattatgaaa aagccaagca
     8581 gttggcgctg gaatacgctg aaattttcgc tggtcgcttt tatctggaag tgcaaaacca
     8641 tggcattgat gatgaactgc gttggtacga aattgctaaa aaattgtccg ctgaaatcgg
     8701 tctgccgcgc ctgctaacca acgatactca ttatacttta ccggaacatt gggaagctca
     8761 cgatgcccat ctttgcattg gcaccggcaa agaaatgagc gatcctgacc ggatgaaata
     8821 cgaacaacat gagtattggg ttaaatcggc tgaagagatg gcggctcttt atcccaacga
     8881 tcctgagata atcgaaaata cgctgaaaat tgccgaacag tgcgatttta atctggagat
     8941 gaaccaatat tatctgccgg aatttcctat cgcggaatcc gctaaggtaa aaactgccga
     9001 tgaatatctg gctcaactga tcgaggaacg acttccaggt cgctatccag aaattacacc
     9061 agaaattcag gagagagtgg attatgagtt gggagtaatc cgccaaatgg gtttcgctgg
     9121 ttattttctg attgtccaag attttgtgca ttatgctaaa aataataaca tcccggtcgg
     9181 accggggcgt ggttcagccg ctggcagtat cgtggcatat attttaggca ttactaatat
     9241 tgatccccta aaatataaac ttatattcga gcgtttccta aatcctgagc gtatctctat
     9301 gccggatatt gacattgatt tttgcgatac caaacgcggc gccgttattg attatattcg
     9361 caagaaatat ggcgagaact gtgtggctca gatcattact ttcgggaaaa tgaaagcaag
     9421 agcggtgatt cgcgatgtcg gacgggttct cagtatgccg ctggcggaag ttaaccgaat
     9481 cgctaaactg gtcccagaac aaattaatat cactctcgaa accgccatta acaatactcc
     9541 ggaactgcag gaagccgtca atattgatga acagcatcag aaattgtttg caatttccaa
     9601 ggttttggaa ggcttgaacc gtcacgcttc cacccatgcc gccggagtcg taattgcccc
     9661 gggcaatctg acggattacg ttccacttta ccagtcaagc aatggcgaag tcaccaccca
     9721 gtatgatatg aaatgtattg atcagatcgg cttgctcaag gttgatttgc tgggaatccg
     9781 caacctcagc gttattgaca atacactcgc gatgttaaaa gctaagggca ttgaactcga
     9841 tatagataag attccggaaa aggatgaaga aacccttgct cttttcggcg aaggccgcac
     9901 gattggcatt tttcagtttg agtcttccgg aatgcgggag tacctgaaga agctgaaacc
     9961 gtccggcatc gaagacttga tcgcaatgaa tgcgctctac cgaccgggac ctatggaaat
    10021 gatcaatgac tttattgatc gcaagcaagg tcgcaaacca attacttatt tgcatccctt
    10081 gctggaaccg atcctcgtgg atacctatgg cattatcgtt tatcaagagc aagttatgca
    10141 aattgcccat caggtggctg gcttctcgct ggctaaagcc gatttgatgc gcaaagctat
    10201 gggcaagaag cagaaaaaaa ctatggaatc ccttaaaaag gaattcgttg aaggtgcgat
    10261 caaaaacggc atcaagcgtt cggttgccgt ccagatttat gaattaattc agcgtttcgc
    10321 cgagtatgga ttcaatcgca gtcattctgc cgcttatgcc attctcgcct atcagtgcgg
    10381 ctatttgaaa gctcactatc cggctgaatt tatggcggct aacctctcga gtgaaatcaa
    10441 tgacaccgac cgaatcacga ttcttgccaa tgaagcccgc aatatgggtt acgaaatctt
    10501 accgcccgat gtgaattata gcgaagttcg cttcattccg gaagggaaag caatccgtta
    10561 tggtttgcat gctatcaaga acgtcggcga gaaagccgct gaaaatatcg tcgccacccg
    10621 ccagaccgaa aagcaatttc gtactctttt tcaaatggta gcggctctgg atttgaaaag
    10681 tgtcaatcgt aaagttctcg aaagcttaat tgcggctggt gcaatggact cgctggaagg
    10741 cactcgcggt cagaaatttg ccgccattga cactgctatt caatacgcta cgcatattca
    10801 agaagaaaaa aacaataatc aggtgaatct ttttggaatc gctaacggtg cgccgcaggc
    10861 tactttgagt gaaccgcgtt taccggatgt agctccctgg gaggattccg aaaagtggaa
    10921 taaggaaaaa gaactgctcg gtatgtattt aagtgggcat ccgctattgg agtattcgac
    10981 ggaaatagag tccttttcca attacgattt caccgagccg ctatccaatc tggataagtc
    11041 caatgtcaaa ttaggcggac tgattgccag catcaaaact atgaccgatc ggaaaggtcg
    11101 cccgatggct ttcatcaatc ttgaaagcct gaacggcaag gtcgaattaa tcgcctttgc
    11161 cgatatttat gaaaagtatc gtgaacattt aaaaatcaat tatcccatct ttgtcagtgg
    11221 aaatgtcaat tgccggaatg aagacgacgc caagattatc gtcgaggaaa tcagcccctt
    11281 gcgcggtttg cttaaccgca aatccaaaaa acttcatgtg cgcatcaatg tcaatcaatt
    11341 tggtactgaa gatctggaaa atctcaaaaa attactcgcg aaacataccg gcgagtgtca
    11401 gctgttcctg catctctatg acggtaatgg cgtcaacaaa tcaatccgtt cgggaaatct
    11461 gagagttagc gccgatcgcg aactgctcga tattctaaag aaaacttttg gcgaagataa
    11521 tgcctgggtg gaataatgat cgcagttatt caaagagtca gccaagccca cgtgaccgtc
    11581 gaaaacaaaa ttgtcggccg gattgggccc ggattagtga ttctactggg tgtttttcag
    11641 aatgatggtg atgcagatgc cgcttttcta gctgataaaa ttgctggttt gcggattttt
    11701 ggggatgaac agcaaaaaat gaatctctca atcaaggatg ttggtggcgc ggcattggtt
    11761 atttcgcaat ttaccttatg cggcgattgg cgcaaaggcc gccggccaag ctttatctca
    11821 gccgctgatc cagaaaccgg acggcggctc tacgagaaat ttattgctga tttaaaaatt
    11881 gccggggttc cgaccgaaca gggtatcttc ggagcaatga tggcggttag tttagtcaat
    11941 gacggtccgg taactttcgt gcttgatagt aaattacggt gaaattatgc gatctaagga
    12001 acctgccgtc tatagacttc cgataacagc ttggcccgaa agcgatcgcc cgcgtgaaaa
    12061 gctgatgaaa gccggaccta tgaaattgac ggaagccgaa ctgctggcta ttttattacg
    12121 tagtggagcc ggaggcaatt cggctctgga tgtcgctcgt gagattctgc gtaaaagcgg
    12181 cggactgaat gctctggctc aaatggatta tcacgatatt ctgaatctga aaattagagg
    12241 tatcggccca actaaagccg tcaccattgc ggcaggcttg caattagcac gccgtctgca
    12301 ggctgtcagc gtatctccgc cagaaatgat tatccataaa agcgatgagg tggcaaaaat
    12361 ttatattccc cgtctgcagg atttgaaaaa agagattttt atggttgtct tgttagctag
    12421 taacaacaag attatccgtg atgcaatcat ctctgaggga attttaaatg cgtcagtcgt
    12481 tactccacgt gaggttttcc gcgaagccat tctggggatg gcggcagcca tcattttaat
    12541 tcataatcat cccagcggga atccggagcc gagcaatgaa gacctccaat tgactcgcca
    12601 gatgaaggaa gttggtcaag cgatgaatat tccggttctc gatcacatta tcatcgctgg
    12661 cgctcaatac accagttttg cggataaaga gttgctctga taaaatgttt atattaattt
    12721 tgaagcagga aaaagctatt acaaatgaac actaataata ctcaattcca aattacggga
    12781 aaggttcaat ctggtataat acctactcac aggcttacag caacttggca actttttaat
    12841 aattacagat tctccttcct caccatagac ggaagtaatt aaaaaacaag gaagagagtg
    12901 agaatcaaaa gaggtttgtg atggaaatta tcgaacgagc gactctgcaa caacatatta
    12961 ccaatacgcc ggacagttat ttgcgtcccc tcttggcggc gctttatctg gaagatcagg
    13021 actgccaatc ggcgatcgaa ctctgccgcc aagaccttgc cattcatccg ttctccacga
    13081 ttggacacta catttgggcg ctggcggctt tagcaatgaa tgatctgaac caagccgcta
    13141 ttcatcttcg tgcaattatt cagattgatg ggaaattttt acaagcctac tacaaattga
    13201 ccgaactggg gggagattat cttactcaag cggaattaaa atattgttat gaaaaaattt
    13261 gcgacttgaa tccctatgat aaaaaaagtg ccgatagatt gtctgccttt cctcttgaat
    13321 tacaatctca gaaaccgcag tcgccagcca aaccggagcg aactgatcag gtcaaagaat
    13381 ccagcgaaaa ggcgataatg gctgagccaa tcgaatcagc cccggaaatt gatccgctca
    13441 ccggcgctaa ccgtctttct caatttttcg agtcagtaat aaaaactacc gtaacaccaa
    13501 attcttctga aattaaacca acacccgtcc cggaagaaga agcccatttc gggccaatcg
    13561 aaaaaacggc gatagaaacc gagccgacag tggaaccgga accaaccatc agcttcgatg
    13621 aacccttccc ggaagaacct caggttaaag aatcatccgg ttcttcctcg attagcgact
    13681 tattcagtcg tctgcgcgaa aagccattcg aggaagtaca aaaagaaaat tggctgaagg
    13741 aacatttgtc tggtgaaacg ccgtcgcctg caatgccgac tataactgaa tctgacaaac
    13801 cggctgtcga acctcaagcc gtctctcagc ctcagagcga aacaaaagcg acggacattc
    13861 cggctaaaag taaggcgact gcaaccccca aagttgcgcc caccaaagca ggcaaagcca
    13921 gccaaaaacc gaaatcagct tctaataagc aacgtcagtc gcctcaaccg aaaaaaagcg
    13981 ccgcctcgcc tggtaatatt tcatttccaa ttcccacctg gaccttggtt gatattctaa
    14041 tgaaacaaaa actctatgaa caggcactta acattctcga tataatcgaa tccaagtcta
    14101 aaaatgaaaa agacctggaa aaagtccaag caagccgagc gaaaatcgtg cgattaatgg
    14161 ctgaggaaca atccggcgaa gcttgatttc agcagaggaa atattttaat ttcttaggag
    14221 cacttaaaca ttgatgcact ccataagaac tcatatatta aagatcatcg ataatcttaa
    14281 ccttatccca ttggtacttt tttcgtaact cttccatctc tttcttctca gtttttatcc
    14341 tctcataata actaccaatt ttacaatctt agtttgattg gggtcttcat aatttaccat
    14401 ttagggctcc aaattgattt gttaaatgca taggttttgt caaatatcac cctaaatatc
    14461 tgttattaca atatattgga tactttttca ggaaacccta tttagggtta acgaaaaatt
    14521 ttatttttag tcagtattaa tcccaatcaa ttctccttct ttaaaacgtg ggctcgccgt
    14581 ttgtgacgac gagcaccact ctatgtcaac tgcccctttt cggtgtctat ttacaagcta
    14641 tttttttaaa agtcaaggat attttttgaa agttatgtct gatctagtga catggtatct
    14701 attatataga actcattctt gtcctcaaat ataatctacc ctcctaatga ccaatgacca
    14761 ttctcagcct tatttttcct ggtcttggaa aaataaaccg ccaagttcgc caagaaccgc
    14821 taaatgagaa aaatcccaaa tctcaaattc caaatcacaa atagccaaac caatttctgc
    14881 gaaactgtct cctgcctcct ttctcctttt ctcagcctca gcctttttac taattactaa
    14941 cgaccaatga ccaatgacta atgaccaact ttcctgttct tttttctatg ccgaaaaaag
    15001 aaccaaaaaa ggcagcggct aaagaacatc tcacgggggt ttgctcagtc ccagcgaatg
    15061 gaatttaagg cgcaaaagtc atcaagttca taaaacctta gtgataaagg aaagggtaat
    15121 ggataattac gaaagtgcta ttacagcgct aaaaattcca ttttttccgc tgggacttcg
    15181 cttcccagcc ggaaatgttc ttaatgccgt aatggtaaat tggatggtta attttagttg
    15241 tcgaggatgt ctccttttct ccctatctcc ctatctcagc ctcaatctct ttactcaacc
    15301 tctttactca gcctctttac taatgaccaa tgaccaatgc ccaatgacta ttcattgacc
    15361 ggtttggtga taattgtacg ccattccgga agttgccggg cggctttta
//
LOCUS       DAOOMU010000051        15249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4149_length_15249_cov_23.222325, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000051 DAOOMU010000000
VERSION     DAOOMU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15249
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_15249_cov_23.222325"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1469
                     /locus_tag="PLI55_06220"
     CDS             291..1469
                     /locus_tag="PLI55_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB00466.1"
                     /translation="MIYQRKISKSLREHLSLRQITVITGMRRTGKTTLVKQILSEVNS
                     ENKLYIDFERFDERELFMEKNYENIIYALSQRGLDFSQKVWLALDEIQNVPNLDSVIK
                     YLYDKYNIKFILTGSCSFYLKNRVTESLAGRKKIFELFTLDFGEFLTFKEVPFQSGDW
                     KKLNFNASEYERLKMYYDEFVEYGGFPEVVLTKNVFEKRDLVQDILSSYINVDVRTLS
                     DFRDIHNLERLIKLLGTRAGSRIDYSKLAILSGISRPTLYNYLNFLEKTYIIWQMPVL
                     THNPDREIVKANKLFFHDNGILNALSAVSEGVKFENTVFNQLKHWGELRYYSLKNGRE
                     IDFILNGEIAFEAKVSPTAIDLKTLAHIAENLQINQYRLIGKHPTPNFSNFIWAGEIR
                     "
     gene            1466..2653
                     /locus_tag="PLI55_06225"
     CDS             1466..2653
                     /locus_tag="PLI55_06225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB00467.1"
                     /translation="MKIFIVPSWYPSKLSPENGTFFRDQAQILAKYGDDVTVIVDLVH
                     SFKNFLRVKRFSTVKFQPENLGGVIEYRQETLNWFPKRPQKTYFSQRRRLIKLLERAI
                     AERGRPDLVIAHSSLFAGAELARWLKDNDIPMIVTEHLMHFLKPEMLTEFYKDCIHEA
                     YCYADRIVATSSRLRDSIVVQFPEFTPKIVVVPNPVDISAFSPASEGKKALKPFVFLC
                     VALLRPEKRLDLLLRAFATVRQNNPDTQLVLVGDGPQRRNIEHLISNLNLQSSVTLTG
                     YLPQTEIAGWMQKCHTLILPSEVETFGLVLVEAMACGLPVIATRCGGPEDIVTPETGF
                     LVPVNDVTGLTEAMQKMIIEYCQFDSQSIRQTVIDKFSDHKYYWAIHNLYDEIMNNSP
                     KLK"
     gene            complement(2665..4098)
                     /locus_tag="PLI55_06230"
     CDS             complement(2665..4098)
                     /locus_tag="PLI55_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPB00468.1"
                     /translation="MSGNFRKFFKESTLYLGGLVLNRLVSFLLLPIYTNVFDKTQNGI
                     ITIAYAFLGFMVIILPYGTDAALLNFYTGNDRDRKNYFSSAYLLVLISNLLILGLAFL
                     ARNSLSQAVLRVSDPHILVWCLIILFFDTLNGLTLLVLRAEGRPLTFISFSLANVLLA
                     MGLNIFLVVHRRLGLAGIFYSNILTSGMIWLGLLPLVLKRLDLRLISGKYIRNLVRFG
                     FPFLPAGIFAMILDLSDRWFLEYFTDMETVGLYGVGYKLAAVMLVFVLAFNSAWQPFF
                     LKARREQNTPNIIARVSFFVIGGLAFCWLFFSIWMNYIVRIKISGRYLIGAEFWAGTS
                     VTPLIMLAYFFQALYLLQLPSIFLLEKTKWEPVFRGLGAATNIILNIVFIPRLGMSGA
                     AIATVLAYLAMVIAIWFFNRKYYPIKYDWYKIGAIIVLTAAAYLVAIWGDSVIYRVIA
                     TGIGLLGIGGILLLPLKGRILPILKKK"
     gene            complement(4107..5573)
                     /locus_tag="PLI55_06235"
     CDS             complement(4107..5573)
                     /locus_tag="PLI55_06235"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPB00469.1"
                     /translation="MTASIRSLTKQTLIYGVGTILARLVTFLLLPLYTNILPAAEYGL
                     ASLVFAFNGFMIIIFNYGLDSAMMRYYGESTEPAVRKRLLSTSIWLTISTSLILSGLI
                     YGYSGFLGRVLLGDAARGELLRYSAGILFFDAIAHVPFALLRLEERPIRFMSIRLLNV
                     IMMFGLNIYFVAHLQRGVAGIFQSNLITSAVTTTILFILIIPRLSLQFSGESARNLVL
                     FGLPFIPAGLATVCMEMLNRYILEHLMGLEAVGVFSAGFKLGIFMLLLTNAFYYAWQP
                     FFLKAGPQESSRLLFARIFTYFVLVELIFWLVLTALMPEIVRFHIGQIYLIGKEYQSC
                     EPMVPIILMGYVFLGIYQMFLPGIYFEKKTRYLAYLTIFAALVNVGFNFILIPSLGIL
                     GSAFSSLIGYFTLAVCSYFVSQRLFRVPYELRRVGLLFLLTLAGGIPIWLFRLNLPIR
                     IAILVVYPLLLWLAGFFKSSEIAKLKQIFSKTTSHHKN"
     gene            complement(5731..6699)
                     /locus_tag="PLI55_06240"
     CDS             complement(5731..6699)
                     /locus_tag="PLI55_06240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB00470.1"
                     /translation="MSITVEIKQDFPDDQWDRFVYQSVNGTIFHTCQFLSYHPKGRFE
                     DASLIFRQEAELFALFPATRGNWDGYDTLWSHRGASYGGFVYREGLSIKNAYELVEKL
                     IEYARRERIKRIVLTLPPIIYNRRLSNYIDFALIQHGFQYLKREVSSIVSLEENINLN
                     VAKFKPSNRTAFRRAQKLGVVVRESQDYANFYKILKKNLKIRHGVQPTHTLEELVQLK
                     DLFPEKIHLFGAYLADEMVAGVVMFDCNPQVSLAFYISHNEERQECRGVNLLFYEIIK
                     RCIAQGFKFLDFGIFTVNMEPNFGLARFKEGFGSSGILRDTLYLDL"
     gene            6959..>8174
                     /gene="eno"
                     /locus_tag="PLI55_06245"
     CDS             6959..>8174
                     /gene="eno"
                     /locus_tag="PLI55_06245"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPB00471.1"
                     /translation="MSTIIEVNAREILDSRGNPTIEVEVLTESGFYGSAAVPSGASTG
                     EHEAVELRDGDKSRYLGKGTLKAVQNVNDIIAPEIIGEDVLDQAWIDNLMIELDGTPN
                     KAKLGANATLGVSLAVARAAANFVGLPFYKYIGGVNARVLPVPMMNILNGGKHADNNV
                     DLQEFMIFPVGAKCFAEALRMGVETFHQLKSVLKKKGMNTAVGDEGGFAPNLRSNEEA
                     IEVILEAAAKTGYKIGTELFIALDPASSSFYNTETKKYELKSENRSLSSEEMVDYYKN
                     LVDKYPIISIEDGMAEDDWDGWKLMVKKLGNRIQIVGDDLTVTNVERLSRAIKEKAIN
                     SILIKLNQIGTLTETLNAIELAHRSGFTTVISHRSGETEDTTIADLAVAVNSGQIKSG
                     SACRTDRICKYNQ"
     assembly_gap    8175..8274
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8391..8738
                     /locus_tag="PLI55_06250"
     CDS             8391..8738
                     /locus_tag="PLI55_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPB00472.1"
                     /translation="MRKSRPKSIKSKDGSGYLHKLYWILGILCAITLFIIFFVIGDYG
                     LYQIYLLNKQKEQIENHLVELQYTQDSLLIEKTRLESDLQYIEKLAREKYRMAKKGEK
                     VFRVIEKPVERLN"
     gene            8859..9653
                     /locus_tag="PLI55_06255"
     CDS             8859..9653
                     /locus_tag="PLI55_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00473.1"
                     /translation="MEKRFLFCLLLCIGTAQAAFEIHQASPIIIAGGGTISVFPDGSN
                     PASLVEKKGLSTCCNFSNLYGIKNLQTWDIGLFYNHHSKTGLALRLNTLGNLVYQEKT
                     IGFSLARKISSLISLGATVNYYDLTISNYPRTGAIGLNCGLKLFPDSTLGFAILFENL
                     NAPKICDRQEPLPQVFAFGWQWKPIRRGEISGEIFKDTRFPFTWRSGIRFEVFRGCNL
                     LAGVQFNPDRLSGGLELAWYKFQIAVAFLHHPTLPYTVYYQCGLNF"
     gene            9635..11497
                     /locus_tag="PLI55_06260"
     CDS             9635..11497
                     /locus_tag="PLI55_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00474.1"
                     /translation="MRLKFLIGLLFSYLIIAIADAPAFGQNDLSADILDNYFTDNALD
                     IDELQELIYAYRDNPLVWETCRIKDLRALPLNDYIISQLIELKRHYPKSLNLKALVKD
                     TIFTTAEIEAISYFIDFSVKRTKPSQIVNFISLKKQEYTSLQKSLQRARCYSPQGWFT
                     GIIVENDSDEPILGDYWNISLRSPKYFNSLEILGGAYRLQWGQGLLFANNLMSGRSTD
                     ATGNLKTSRSGVSNYLGSDENRYLFGSAAQYTSGHFKLIPFISHHRLDATIDTNGTVK
                     NLRSDGLHISSSQLRAKDALLETLGGASLLGEWDQGSAGILVYNANYSTGLFALNGHR
                     KVSGLSFLHNYEISDWTISGEWVVQETGKKGIIQNLHLSRDHVSFCAGWRYIEPDFFA
                     PLGNPFRKFSSLSGNETGLYSALKIKLPARWRFTGYVDFFRELKNSEPGVAIRNGRES
                     LCGFSRQARNNDLIEAWYKVNRYYHTNLPQQPRDYQLKLHLRHSFSTSFSGEIRTTFR
                     WNDSIPFAESSQALSVVGKVSISKYTRVTAGNTHYFTGSSDLNIYFYEPGLPFRFNMT
                     LLTGTGQRYFLIISRRIGSNCEIAGAVYGRSNVSIHTNDNSFDFSGDLQLTFDL"
     gene            11487..13106
                     /locus_tag="PLI55_06265"
     CDS             11487..13106
                     /locus_tag="PLI55_06265"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPB00475.1"
                     /translation="MTYDIEVCNFTTIVKIFRPYIYFLLLLVNCHNLLADQLVIQSPR
                     PKTGTSISVLYFDNCAYFSVKDFAQALGLRTYNNPETGKIVLYFSGKSVKITAHSAFI
                     MLDARILQMSQPAILVNRDEIFVPMYSFLDLMKRYAIPKLQYYLDKRSPIYELVELPG
                     ATPIPSAPTVTTFGTKPGIIYDIQFEERGNGLTMKISTNATYESSDFSSFFRGDEWFY
                     LTILGATCDSAALSRRDPIPSVSIVEAIPVGNSVQITLKLKQKYDRANAYYDARSGKI
                     LVTLNVPLTSATLKKIEEVKNTWIVDTIVIDAGHGGKDSGTPGRWGYKHEKDIVLDIA
                     LRLGKLLEQRNDLKVVYTRKNDEFIPLWKRTRIANDSAGKLFISLHINALPSGTRSSA
                     EGIELYLQNPTIRTQESIEVARLENEVIKLETSEDKAKYKDYDNPSHILANMVYNTYL
                     HDSEKFAEILSRNISEYVPQKNRGVKQANFYVLVGASMPNLLCELGYNDNRNDAKKLN
                     DPAHRQKIALALYKSVIEFKEYCDQSVAQSK"
     gene            13078..13914
                     /gene="murI"
                     /locus_tag="PLI55_06270"
     CDS             13078..13914
                     /gene="murI"
                     /locus_tag="PLI55_06270"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPB00476.1"
                     /translation="MTNQWLKVSNKQNLPIGVFDSGLGGLTVVKQLFRQLPQEQIYYF
                     GDTARVPYGNKSVETVQKFAIQIARFLAERSVKMIVVACNTASSVALELLQDQFDLPI
                     IGVIEPGARSALAISPTKKIGVIGTTGTINSGKYVEVLTALDKSVQVFSQACPLFVPL
                     VEEGWGESPVTEIVAREYLQNLVPKIDTLILGCTHYPIIKNVIQRIIDSNIQIIDTAI
                     ETANQVRLILNQQGLLNDLERTPEHRFYVSDLPQKFEEIAMRFLGEPIANVERINLAD
                     CC"
     gene            13914..15212
                     /locus_tag="PLI55_06275"
     CDS             13914..15212
                     /locus_tag="PLI55_06275"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPB00477.1"
                     /translation="MSSSDAADLRPILSYLYSLNGRGIKPGLERIQKLLDAIGNPQNA
                     YKVIHVAGTNGKGSTCAIMANVLQTAGYRVGLYTSPHLLNFNERIRINGQKIPDAEIA
                     LFTERWRKLIDELNCSFFEATTAMALDYFKKNKVDYAVLETGMGGRFDATNVTLPEVA
                     VITPIGRDHQEFLGRRLAQIAYEKAGIVKRGIPCIVARQTPRVLNFLSKEINASGGDF
                     YYAPEQCQITNIQRFPERQVLSLIVNNLKIDTVKFPLIGDHQLINLQAAVSALMRVKD
                     LELSAEVLRQGITGVIWPGRLHILSHKPLIYFDVGHNQHGIRQVVKALKQIFPTQPVH
                     LVLALGSVKKYDRIGKIIQPLGGDIYLTEIPDHPSVSAEILADVIRKQIPSRLIYVDN
                     KLERLLNFLEIQIKSDEILLIAGSHYLAPQVLPFYGVSLT"
BASE COUNT         4587 a         3351 c         3107 g         4104 t
ORIGIN      
        1 ggcagacatt gccgttcaat tcatgtgccc gtttccagta agtaagcgtg ccgaccgtat
       61 tttctggtga aatatataga atcttcaaca cgattgaaaa ataagcattt caggattaat
      121 tgggaatcga aaagttcttc aattaaaatt cctttaaatt ataatcagtg ctaaaaccat
      181 ttcaactaaa ttttttgaca tattacttta caaattgtga gtctatttgt aaaatataat
      241 ttacaaatag taatgaattt tgtaaattaa tgggttaatg gagaactatg atgatatatc
      301 agcgcaaaat ttccaaaagt cttagggaac acctgtcact gcgccagatt acggtaatta
      361 ccggaatgcg tcgcactggg aaaacaactc tggtgaagca aatattatca gaggtaaatt
      421 cggaaaataa gctatatata gattttgagc gttttgatga acgcgaatta ttcatggaaa
      481 agaattatga aaatatcatc tatgctttgt cccagcgtgg tctggatttt tctcaaaaag
      541 tctggctggc tctggacgaa attcaaaatg tgccaaacct tgacagcgtc atcaagtatt
      601 tatacgacaa gtacaatatt aaattcattt tgacaggctc gtgttcgttc tacctaaaaa
      661 atcgtgtcac tgaatcgctg gctggtcgga aaaaaatatt tgaattattc acccttgact
      721 tcggcgaatt cttgactttt aaggaagtgc cattccagtc cggcgactgg aaaaaactga
      781 attttaacgc ttcagaatac gaacgcttaa aaatgtacta cgatgaattt gtcgaatatg
      841 gtggatttcc ggaagtagtc ctaacaaaaa acgtatttga aaaacgtgat ctggttcaag
      901 atatattaag ttcctatatc aatgttgatg ttcgtaccct ttccgacttt cgggacattc
      961 ataatctaga aaggctaata aaattgctag gtactcgcgc tggaagtcgg attgattatt
     1021 caaaactagc aatcttatca ggaatttcgc gcccgacgct ttataattat ctcaactttc
     1081 tggaaaagac ttatattatc tggcaaatgc cggttctgac tcataatcct gatcgtgaaa
     1141 ttgtaaaagc caataaattg tttttccatg ataatggtat tcttaatgca ctctcagctg
     1201 taagcgaagg agttaaattc gagaatactg tttttaacca attgaaacac tggggtgagt
     1261 tacgttacta ttctttgaaa aatgggaggg aaattgactt tatcctgaat ggggaaatag
     1321 ctttcgaggc gaaagtaagc cctacggcaa ttgatttaaa aactttagct catatagcag
     1381 aaaacttgca aatcaatcaa tatcgcctta tcggaaaaca ccctacccct aattttagca
     1441 actttatctg ggctggagaa atacgatgaa aatcttcatt gttccgtcgt ggtatccatc
     1501 aaaactctcg cctgaaaacg gcacattctt ccgcgaccag gctcagatac ttgcgaaata
     1561 cggcgatgat gtcacggtaa tcgtagatct ggtgcattct tttaaaaact ttctgcgggt
     1621 taaaagattt agtactgtca agttccaacc ggaaaatctc ggcggcgtta tcgaatatcg
     1681 tcaggaaacc ttaaactggt ttccgaaacg acctcaaaaa acttattttt cccaacggcg
     1741 gcgcttgatc aaactacttg aaagggcgat tgccgagcgc ggccgaccgg atttagtcat
     1801 cgctcactcg tcattatttg ccggagcaga actcgctcgg tggctgaaag acaacgacat
     1861 cccgatgatt gtcaccgaac atcttatgca ttttttgaaa ccggaaatgc taactgaatt
     1921 ctataaagac tgtatccacg aagcgtattg ctacgccgac cgaatcgtgg ctaccagttc
     1981 ccgattgcgg gattcgattg tagttcaatt cccagaattt acccccaaaa tcgtcgttgt
     2041 cccgaatccg gtggatatat cggctttttc tcccgcttcc gagggaaaaa aggcactaaa
     2101 accatttgtc tttctttgtg tggctcttct gcgccccgaa aaaaggctcg accttctgct
     2161 gagagctttc gccaccgttc ggcaaaataa tcctgatacc cagcttgttc tcgtaggaga
     2221 tgggccgcaa cgtcgaaata tagagcacct aatttctaat ttgaacctgc aaagttccgt
     2281 tactctgacc ggttatttgc cgcagaccga aatcgctggc tggatgcaaa aatgccacac
     2341 gctaatttta cccagcgagg tcgagacttt tggcctggtt ctggtcgaag ctatggcttg
     2401 cggtctgcca gtcattgcaa cccgctgtgg tggcccggaa gatattgtca cccccgaaac
     2461 cgggtttttg gtgccagtta atgacgttac tggtctgacc gaagcaatgc aaaaaatgat
     2521 tatcgaatat tgtcaattcg attctcaatc cattcgacaa acagttatag ataaattcag
     2581 cgatcataaa tattactggg caattcacaa tctctatgat gagattatga ataactctcc
     2641 taaactaaag tgaattttcg tttgttactt ttttttcaga atcggcaata ttctaccctt
     2701 caatggcaag agaagaattc caccaattcc caataacccg attccagtag ctattacccg
     2761 gtaaataacc gaatctcccc agatagcaac cagataagcc gccgcggtca aaacaatgat
     2821 cgctccaatt ttataccaat cgtattttat cggataatat ttccggttaa aaaaccagat
     2881 ggcaattacc atcgcaagat acgccagaac agtcgcaatc gccgccccgc tcattcccag
     2941 gcgcggtatg aacacaatat ttagaattat attagtagcc gcgcctaatc cgcgaaaaac
     3001 cggctcccat ttagtttttt ccagaagaaa aatactcggt aattgcaaca aataaagtgc
     3061 ttgaaaaaag taagccagca ttatgagcgg tgtgacactc gtaccagccc aaaattccgc
     3121 accgatcaaa tatcggccgg aaattttaat cctcacgata taattcatcc agatactaaa
     3181 aaacaaccaa cagaaggcca atcccccgat aacaaaaaag gaaaccctgg caataatatt
     3241 aggagtattt tgctcacgcc gcgctttcag gaaaaagggc tgccaggcgg aattaaacgc
     3301 cagcacgaaa accagcataa ccgcggctaa tttatagcca acgccatata atccaaccgt
     3361 ttccatatcg gtaaaatatt cgaggaacca gcgatcggat aaatccaaaa tcattgcgaa
     3421 aattcctgcc ggtagaaagg ggaacccgaa cctgactaaa ttcctgatgt atttgcctga
     3481 aatcaagcgt aaatccagcc gttttagtac cagtggtaaa agtcccagcc agatcattcc
     3541 cgatgttaga atattactgt aaaaaattcc tgccaagcca agcctgcgat gcactaccag
     3601 aaaaatattc aatcccattg ccaacaagac atttgccaga ctgaacgaga tgaaagtcag
     3661 cgggcgacct tccgctctta agacgagcaa ggtcaagcca ttcaaggtat cgaagaataa
     3721 aataatcaag caccaaacta aaatatgggg atcgctcacg cgtagtacgg cttgcgacag
     3781 actatttctc gctaaaaaag ctaatcccag aattagcaaa ttcgaaatca gtaccaacaa
     3841 ataggcgctc gaaaaatagt tcttacggtc gcgatcgttt ccagtataga aattcagcag
     3901 ggcggcgtcc gtgccatatg gtaaaataat caccataaat cccagaaaag cataagcaat
     3961 cgttataatg ccgttctgag ttttatcgaa aacgttggtg taaatcggaa gtaatagaaa
     4021 acttacgaga cgattcagca ctaagccgcc taagtatagc gtcgattctt tgaaaaattt
     4081 tctgaaatta cccgacatat tcatattcag tttttatgat gcgaggtagt tttggaaaat
     4141 atctgtttca atttcgctat ctcggaactc ttgaaaaaac cagccagcca taaaagaagc
     4201 ggataaacta ccagaatagc gattctaatc ggcagattta gtctgaacag ccaaatagga
     4261 atcccaccag ccagagttaa tagaaacaaa agcccaactc gccgcaattc ataaggcacc
     4321 cggaaaaggc gttgcgaaac aaaataactg catacagcta aagtaaaata tccaatcaag
     4381 gatgaaaaag ccgaaccgag aattccgagg ctcggaataa gtataaaatt aaagcccaca
     4441 tttaccaaag ccgcgaaaat agtcaggtaa gccagataac gggtcttctt ttcaaaataa
     4501 attcccggga gaaacatctg gtatattcca agaaaaacat atcccatcaa aataatcggc
     4561 accatcggct cgcaagattg atattcctta ccgattaaat aaatctgacc aatgtggaaa
     4621 cgcacaattt ccggcattag agcagtcagc accaaccaga aaatcagctc aaccaacacg
     4681 aaataggtga aaatccgagc aaataacagg cgcgacgatt cctgcgggcc agctttcagg
     4741 aaaaagggtt gccaggcgta gtaaaatgcg ttcgttaaaa gaagcataaa tattcccaat
     4801 ttgaaaccgg cgctaaaaac gcccaccgcc tccaaaccca tcaagtgttc cagaatataa
     4861 cggttaagca tttccataca aaccgtagct agcccggcgg gaatgaaagg cagaccgaaa
     4921 agtaccaaat tgcgggcact ttctcccgaa aactgcagac ttaaccttgg aataattaaa
     4981 atgaaaagta tagtagttgt aacggcagat gttatcagat tactctgaaa gattccagct
     5041 actccgcgct gaagatgagc gacaaaataa atattcagcc cgaacatcat aataacattc
     5101 agaagccgaa tactcataaa tcgaatcggc cgttcttcaa gacgtagaag cgcaaacggc
     5161 acgtgcgcaa tggcatcgaa aaaaagaatt cccgccgaat aacgcaggag ttcaccacgc
     5221 gccgcgtcgc ctaataaaac gcgcccgaga aatccactgt aaccgtaaat caaaccactc
     5281 agaatcaggc tggtcgagat cgttaaccag atcgaagttg ataacaagcg cttccttaca
     5341 gccggttcgg tgctttcgcc ataatagcgc atcatcgccg aatccagacc gtaattgaaa
     5401 attattatca taaacccgtt gaaagcaaaa accagagacg ccaaaccata ttcagccgct
     5461 ggcagaatat tcgtatataa aggcaatagt aaaaaagtca ccaagcgggc taaaatggtg
     5521 ccgacgccat aaatcagagt ttgcttggtg agactacgaa tacttgcggt catattaatt
     5581 aaagatcgaa aacgcccaaa tatacgaact tttccgcaga atcagcgcca actgtaggac
     5641 aggcattctt gcctgtctat tcctattcac caagtactta ctaattcccc gtcgtttcct
     5701 tgctttatta taaaaccgct aaaatttatt ctaaagatcg agatataatg tgtcccgcag
     5761 aatgccgctc gaaccaaatc cctccttaaa tcgagctagt ccgaaattcg gctccatatt
     5821 tacagtaaaa ataccaaagt cgagaaactt aaaaccctgc gcaatacatc tttttataat
     5881 ttcataaaaa aggagattga cgccgcgaca ttcctgccgt tcttcgttgt ggctaatgta
     5941 aaaagccagc gatacttgcg gattacaatc gaacattacc actccggcga ccatttcatc
     6001 ggccagataa gccccgaaca aatgaatctt ttcgggaaat agatccttta attgcaccag
     6061 ttcttccaat gtatgagttg gttgaacgcc gtggcggatt ttgaggtttt tctttaaaat
     6121 tttataaaaa ttggcataat cctgcgattc ccgcacgacc acgcctaatt tttgcgcccg
     6181 ccggaaagcc gttcggttag atggtttaaa cttcgcgaca ttcaggttga tattttcttc
     6241 caatgaaacg atactcgaaa cctcacgctt caaatactga aagccgtgct gaatcagcgc
     6301 aaaatctata taattggata aacgccggtt ataaataatc ggtggtagag ttagcacgat
     6361 gcgcttgatt cgttcccgtc gcgcatattc aatcaatttt tccacgagtt cataagcatt
     6421 tttaatacta agcccttcgc ggtaaacaaa tcctccgtaa cttgccccgc gatgcgacca
     6481 gagggtatca tagccgtccc aattcccgcg cgtcgccgga aatagtgcaa acagctccgc
     6541 ctcctgacga aaaatcagcg aagcatcttc gaagcgcccc ttgggatgat acgatagaaa
     6601 ttggcaagta tggaagatag tcccattgac gctctggtaa acgaacctat cccactgatc
     6661 atctggaaaa tcctgtttaa tctcaaccgt aatggacatt ttatttcctc aaaaaagtcg
     6721 gcttgaattt aaattcaact accggctaaa tcaaatccaa ttcacaaaac ggacatttca
     6781 tcaataatct ctggatgaaa ttttatgaga ttgtcggtga ttttaggcaa ctattttgat
     6841 gatttcttaa ttttctttgg tcggcgttcg cgcttttatt gaaaaatatc cgagagctga
     6901 ttaaattgac agcgtatttg aattatctta aaaaaccgaa gcaaataagg agtaattaat
     6961 gagtacaatt atcgaagtaa atgcccgtga aattctggac tcgcgtggta atccgaccat
     7021 cgaagtagaa gtcttgactg aaagcggttt ttacggcagt gccgccgtcc cgtccggcgc
     7081 ttccaccggc gaacacgaag ccgtggaatt gcgcgacggc gacaaatcgc gctaccttgg
     7141 caaaggcact ttaaaagccg tgcaaaatgt caatgacatc attgcacccg aaatcatcgg
     7201 agaagatgtt ctggatcaag cttggatcga caatcttatg atcgaactcg atggaacacc
     7261 caacaaagcc aaactcggcg ctaatgcaac tctcggcgtt tccctggctg tagctcgggc
     7321 ggcggctaat ttcgtcggct tgccgttcta taaatacatc ggcggcgtca acgcccgcgt
     7381 tctgccggtt ccgatgatga acatcctgaa cggcggtaag cacgccgaca ataatgttga
     7441 tctacaggaa tttatgattt tcccagtcgg cgccaaatgt ttcgccgagg ctctgcggat
     7501 gggcgtggaa acctttcatc aattaaaaag tgtcttgaag aaaaaaggta tgaatacagc
     7561 tgtcggagat gaaggcggat ttgcccccaa tttgcgcagt aatgaagaag ctatcgaggt
     7621 gattttagaa gccgccgcca agactggcta taaaatcggc accgaattat tcatcgctct
     7681 cgacccagcc tcatcctcat tttacaacac tgaaacgaaa aaatatgaac taaaatcgga
     7741 aaatcgcagt ttgtcttctg aagaaatggt tgattattat aaaaatttag tggataaata
     7801 tccgattatt tccattgaag atggtatggc ggaagacgat tgggacggct ggaagctaat
     7861 ggtcaagaaa ctcggcaatc gaattcaaat cgtcggcgat gatctaaccg ttaccaatgt
     7921 cgagcgtcta tcgcgcgcca tcaaggaaaa agccatcaac tcaatattga tcaagctgaa
     7981 tcagattggt actttgactg agaccctgaa cgccatcgaa ctggctcatc gcagtggatt
     8041 cactaccgtc atttcccatc gcagtggcga aaccgaagac acgactatcg ccgatctagc
     8101 ggtggcagtc aattccggtc agattaaaag cggttcagct tgccgcacgg accgcatttg
     8161 caaatacaac cagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncagatt
     8281 aaaagcggtt cagcttgccg cacggaccgc atttgcaaat acaaccagct cctgcgaata
     8341 gaagaagagc tagctgacaa cgccattttt ggaggaaagc gcctatttaa atgagaaaaa
     8401 gccgtccaaa atctattaaa agtaaagacg gttccggtta tttgcacaaa ctttactgga
     8461 ttctcggaat actttgtgca attacccttt ttatcatttt ttttgtaatt ggcgattacg
     8521 gtctatacca aatctactta ctcaataagc aaaaagaaca gattgaaaat catctagtag
     8581 agttgcaata tacgcaggat tctttattga tcgagaaaac ccgattggaa agcgacctgc
     8641 agtatatcga aaaattagcc cgtgaaaagt atcgaatggc taagaaaggc gaaaaagtct
     8701 ttcgcgttat cgaaaaaccg gttgagcgtc taaattaacc gagcgcgatt actgctctga
     8761 aaattggtgt taaatcacag aatttatatc tttcaccggt tgaattgata aattaaagcc
     8821 cgttctcaca acctgcgggc gctttgaagg aggaaaatat ggaaaagcga tttttatttt
     8881 gcttgttgtt gtgcattggt acagctcaag ccgctttcga aattcatcag gcatctccga
     8941 tcattattgc cggtggtgga actatatcag tgttccccga tggatccaat ccggcatcct
     9001 tagttgagaa aaaaggctta tctacctgct gtaacttctc caatctatac ggtattaaaa
     9061 acctgcaaac ctgggacatt ggtctatttt acaaccacca ctccaaaacc ggcttggctt
     9121 tgcgactaaa cacattaggg aatctggttt accaggaaaa aacaatcggt ttttccctcg
     9181 cccgcaagat ttcatcgcta atttctctcg gagcaaccgt caattattac gatttgacaa
     9241 tttccaacta tccgcgaacc ggtgctatag gactgaattg cggcttaaaa ttgtttcccg
     9301 actcgactct cggcttcgct attttatttg aaaatctcaa tgcacccaaa atttgcgatc
     9361 gccaagaacc tttgcctcag gttttcgctt tcggttggca atggaagccg attcggcgcg
     9421 gtgagatatc gggtgaaatt ttcaaggaca cgcgttttcc attcacctgg cggagcggaa
     9481 ttcgatttga ggtatttcgc ggatgcaatt tgctagccgg agttcaattc aatcccgatc
     9541 ggctttcggg cggtttggaa cttgcctggt ataaattcca gatagccgtg gcgtttttac
     9601 atcatccaac tttgccttat accgtttatt atcaatgcgg cttaaatttc taatcggact
     9661 tctatttagc tatctgataa ttgctattgc cgatgccccg gcatttggtc aaaatgactt
     9721 atcggcagat atcctcgata attattttac cgacaatgct ttggatattg acgaattgca
     9781 agaattaatt tatgcttatc gggacaatcc gctggtctgg gaaacttgcc ggattaagga
     9841 cttgcgcgct ttgccgctaa atgactatat catatctcaa ttaattgagc taaaacgtca
     9901 ctaccccaag agcctaaatc tgaaagcact ggtaaaagat acaattttta caactgccga
     9961 aattgaagcc atcagctatt ttattgattt ttcagtgaaa agaacgaagc cgagccagat
    10021 agtaaatttc atttccttaa aaaaacagga atatactagt ttacagaaat cactccaacg
    10081 agcgcgctgt tattcgcctc aaggttggtt cacaggaata attgtggaaa acgatagcga
    10141 tgaacctatt ttaggtgatt attggaacat cagtctgcgt tctccgaaat attttaactc
    10201 tttagaaatt ctgggcggag cctatcgctt acaatggggc cagggtttgc tttttgctaa
    10261 taatctaatg agtggtcgca gtactgacgc aaccggtaat ctgaagactt cgcgatcagg
    10321 ggtaagcaat tatctggggt ctgacgaaaa ccgctatctt ttcggttcgg ccgcccagta
    10381 cacctctgga catttcaagt taatcccctt catttcacat caccggctcg atgctactat
    10441 tgacactaat ggaacggtca aaaatttgcg ctccgatggc ttgcatatta gctcaagtca
    10501 actccgagct aaagacgcct tgctggaaac attaggagga gcaagtttgc tgggcgagtg
    10561 ggaccaaggc tcggctggaa ttttggtcta taatgctaat tattcgactg gtttatttgc
    10621 cttaaacgga catcgcaaag ttagcgggct atcattttta cataattacg aaataagcga
    10681 ttggacgata agcggtgaat gggtagtcca ggaaaccggt aaaaaaggaa taattcaaaa
    10741 tcttcatcta agtagagatc atgtaagttt ttgtgcgggc tggcgctata tcgaacctga
    10801 ttttttcgct ccgctcggca atccttttcg caagttctcc agtctttccg gcaatgaaac
    10861 cggtttatat tccgctctga aaatcaaact accggctcgc tggcggttca ctggctatgt
    10921 tgatttcttt cgtgaattaa aaaattccga gcctggtgta gcaatccgga atgggcggga
    10981 atccctctgc ggatttagcc gtcaagctcg caataacgac ttaattgaag cttggtataa
    11041 agtcaatcgc tactatcaca ccaatctgcc tcaacaaccg cgcgattatc agcttaaatt
    11101 gcatctcagg cattccttta gcacttcgtt ttccggtgaa atcagaacaa ctttcagatg
    11161 gaacgattca attcctttcg ctgaaagtag ccaggcactt agcgttgtag gaaaagtctc
    11221 gatttctaaa tatacccgtg tgaccgccgg gaacacccac tattttaccg gttcgtccga
    11281 cctaaatatt tatttttatg aacctggctt gcctttccgc tttaatatga ctctattaac
    11341 cggcactggt cagcgctatt ttctgataat cagtcggcga atcggttcga attgtgaaat
    11401 agccggcgca gtctatggac ggagcaatgt gtctattcac acaaatgaca acagttttga
    11461 tttttcaggt gatttgcaat tgacctttga cttatgacat agaagtgtgt aatttcacga
    11521 cgattgtgaa aatatttaga ccttatatat attttctatt attattggtt aattgtcata
    11581 acctattagc ggatcagctt gtcattcaat ctcctcgtcc gaaaacaggt acgagtatat
    11641 cggttctcta ctttgataat tgtgcttatt tttccgtaaa agacttcgct caagcgctcg
    11701 gattaagaac ttataacaat cctgaaactg gcaagatagt cctctatttt tccggtaaat
    11761 cagtcaaaat cacggctcat agtgctttca ttatgcttga tgcccgaata ctgcaaatgt
    11821 cacagccggc tattttggtc aaccgcgatg agatttttgt tccgatgtac tcttttcttg
    11881 acttgatgaa gcggtatgcc atccctaaac ttcaatatta tctcgacaaa cgctcaccga
    11941 tttatgagct ggtagaattg ccgggcgcaa ctcctattcc atcagcgcct acggtaacaa
    12001 cttttgggac aaagccgggt atcatttatg atatccagtt tgaagaacgc ggcaacggct
    12061 tgacgatgaa aatttcaacc aatgctactt acgaaagttc agacttttct tctttcttcc
    12121 gcggtgatga gtggttctac cttactattc tcggagctac ctgcgattca gccgcgcttt
    12181 cacgtcggga tccgattcct tcagtgtcaa ttgttgaagc tattccagta gggaattcgg
    12241 ttcaaattac ccttaaattg aagcaaaaat atgacagggc aaatgcctat tacgatgcgc
    12301 gcagtggtaa aattttagtc actttaaatg tgccactgac cagtgctacg ctcaaaaaaa
    12361 ttgaagaagt caaaaatacc tggattgttg ataccattgt cattgatgcc ggacacggag
    12421 ggaaagattc tggcacaccc ggtcgttggg gctataaaca cgaaaaggat atcgtgctgg
    12481 acatcgctct tcgattgggt aaattgctgg aacagcgcaa cgatctaaaa gtggtctata
    12541 ccagaaaaaa cgatgaattt attcctctct ggaaaagaac ccgtatagct aacgattctg
    12601 ctggtaagct ttttatcagt ttgcacatca atgcgcttcc atccggtaca agaagcagtg
    12661 ccgagggaat tgaattatac ctccaaaatc cgactattag gacgcaggaa tctatagaag
    12721 ttgcccgcct ggaaaatgaa gtcattaaat tagaaactag cgaagataag gctaagtata
    12781 aagactatga taatccttct catatcctgg ctaatatggt ttataatacc tacttgcacg
    12841 atagtgagaa attcgctgaa atattatcgc gcaatatttc tgaatatgtg ccccagaaaa
    12901 accgcggcgt caaacaagcc aatttttacg tcctggtcgg cgcttcaatg cccaatttac
    12961 tatgtgaact tggttataat gacaatcgca acgatgccaa aaaactgaac gatccggctc
    13021 atcgccagaa aatcgcttta gccctttata aaagcgttat tgaattcaag gagtattgtg
    13081 accaatcagt ggctcaaagt aagtaataag cagaatctgc ccatcggggt tttcgattcg
    13141 ggtctggggg gattgactgt agtcaaacaa ctttttcgcc aattaccgca ggaacaaata
    13201 tattacttcg gcgacactgc ccgagtgccc tacggtaata aaagcgtcga aaccgtccaa
    13261 aaatttgcga tccaaatcgc cagattctta gccgaacgaa gcgtgaaaat gatcgtagta
    13321 gcttgtaata cagcctctag tgtcgcgctg gaattactgc aagatcaatt cgacttgccg
    13381 attattggcg taatcgaacc aggagcgcgg tcagccctgg caatatcgcc caccaaaaag
    13441 attggcgtca ttggtactac cggaaccatt aattccggaa aatacgttga agtgttaacc
    13501 gctctcgata aatctgtgca ggtcttttcc caggcttgtc ccctgtttgt tccacttgtc
    13561 gaagagggat ggggtgaatc acctgtaacg gaaatagttg cacgggaata tttgcagaat
    13621 ctcgttccaa aaattgacac tcttatttta ggctgtaccc attacccaat tattaaaaac
    13681 gtaattcagc ggataattga tagcaatatc cagattatag atacagccat agaaacggct
    13741 aatcaagtac gcttgattct gaatcagcag gggctattaa acgatttgga aagaacaccg
    13801 gaacatcgtt tttatgtgag cgatttaccg cagaagttcg aagaaatcgc gatgcgattt
    13861 ctgggcgagc caatcgccaa tgtcgaacga atcaacctcg cagattgttg ctaatgtcat
    13921 cctcagatgc cgccgatcta cggcctattt taagttactt atattctttg aatggtcgcg
    13981 gtatcaaacc aggcttagag cgcatccaaa aattactcga tgcgatcggt aatccccaga
    14041 acgcttataa ggtgatacac gttgccggga ctaacggaaa gggttcgact tgtgcaatca
    14101 tggctaatgt tttgcagacc gctggttatc gagtcggact atatacttcg ccacaccttt
    14161 tgaatttcaa cgaacgaatt cgaattaatg gccagaaaat ccccgatgcc gagattgctc
    14221 tattcaccga acgttggcgc aagctaattg acgaactaaa ttgcagtttt tttgaagcca
    14281 caacggctat ggcgttggac tattttaaaa aaaataaagt tgattatgct gttcttgaaa
    14341 ccggtatggg cggacgtttt gacgccacca atgttacgct cccggaagtg gccgttatta
    14401 ctcccatcgg gcgtgatcac caggaatttc ttggtcgccg actagcccag atcgcctatg
    14461 aaaaagccgg tattgtcaaa agagggattc cttgtatagt cgcacgtcaa acaccacgcg
    14521 tcctcaactt tttaagtaaa gaaatcaatg ccagtggcgg cgatttttac tatgctcctg
    14581 aacaatgtca aatcacgaat atccaacgtt ttcccgaacg acaggtatta agtctaattg
    14641 taaataatct taaaattgac acggttaagt ttccgctaat tggtgatcat cagctaataa
    14701 atttacaggc ggctgtcagt gccctgatgc gagtgaaaga cttggaactt tcagcggaag
    14761 ttctgcgcca gggaatcact ggggtgattt ggcccggacg cttgcatata ctgtcccaca
    14821 agccattaat ctatttcgac gttggccata atcagcatgg tattcgccag gtagtaaagg
    14881 cgctaaagca aatatttcca actcaaccgg tccatttagt actggcactg ggtagcgtaa
    14941 agaaatacga tcgcatcggt aaaattatcc agccactcgg tggagatatt tatttaacgg
    15001 aaattcctga ccatccttca gtttcagccg aaatattagc cgacgtaatt cgtaaacaaa
    15061 ttccatccag gctgatttat gtagataata aacttgaacg gctgttgaat tttttagaaa
    15121 ttcagataaa atctgatgaa atattattga tagccggaag ccattacctt gctccacagg
    15181 ttttgccttt ttatggggtg tccttgactt gagatggttg tcattggcaa ttggtcattg
    15241 gtcattggt
//
LOCUS       DAOOMU010000052        15074 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4211_length_15074_cov_17.305879, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000052 DAOOMU010000000
VERSION     DAOOMU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 15074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15074
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4211_length_15074_cov_17.305879"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLI55_06280"
     CDS             complement(<1..563)
                     /locus_tag="PLI55_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB00478.1"
                     /translation="MLKVLIVDDNNESRSLLESFLNRHDYQVFSAVNGKLALDILENN
                     SIDLIISDILMPEMDGYMLCNIVKSDPRWKDIPLIFYSATFTSRQDEIFALKLGASRF
                     IRKPVESDLFLNIIQEVLQNRIEGSLEVAYPRFQDEKDAFKLYSERIIKKLEKNVSEL
                     EESEEHYRAIFENAAIGLYRSTPDGRI"
     gene            complement(576..1670)
                     /locus_tag="PLI55_06285"
     CDS             complement(576..1670)
                     /locus_tag="PLI55_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPB00479.1"
                     /translation="MENKKPTILIVDDVATNLNFLKGLLINDYDIKAANNGQLALKIA
                     RMGAGPDLILLDIMMPGMDGFQVCEELKRDPRTQDIPIIFLTARTATDDIVRGFKSGG
                     VDYLTKPFNPPELLARVRTHLLIKQQKDIILEQNREQKELLHILCHDLANHFTVISMS
                     LDLLKSDSAITDYLSKIKTATTHGIDIINLVREMRALQEKKIEISAVNLSECLAESVL
                     LLGDRFEQKNVKLLIDINHSLWVMAERCSLINSVINNLMTNALKFSFEGGQVEISAAE
                     EDGAVIFKIQDHGIGMPAELMSQIFDITKITSRPGTKGEIGTGFGMPLVKSFVEFYGG
                     EIEVESRDIKKYADDHGTLITIKFINAKNN"
     gene            complement(1672..6288)
                     /locus_tag="PLI55_06290"
     CDS             complement(1672..6288)
                     /locus_tag="PLI55_06290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB00480.1"
                     /translation="MKSINRGFFQKLSFRKKLIVIQLITVSTVIVFFIIFQVIINQIE
                     YQRDVNEKLETTARIIGSNSIPAILFLDNETAENILASLRSESEVVNAWLFDHNRNLF
                     ATFARDGFENYDYPFFDSGRYKLKSRYLIYSSELTQEEVAVGYILIRYQMHPLSSIIL
                     ESLGLGIVVLLIGFGLALVLSFYTGKTVSNPIMALVDTIKEITTKHDYSIRLPKTTQD
                     EIGILYDGFNEMLEQIQKKQEERDKVEKKLREANTIINRSPVVAFTWYNEPTLPVEFV
                     SESVINLFGYTADEFIQGKISYESCIYPEDLPRVNSEVVKYSQTPDVQSFKHEPYRIV
                     TKSGAIKWIDDLSFIIRSESGEITHYQGIVSDITERMEFEELLRQSEIRYRRISSVVS
                     DYVFSAKINQDGVLVTDWVGGAFETITGFTFKEFSEAGGWRARLHPDDLEKDDHDFEK
                     LKKNEPIQSELRFYTKSGELLWLRVYAYPVWDYKNNRLIGVHGAVQNINERKKAEQAL
                     QESEARYRLLFESNPAPIIIYEHNTYNILAVNEAFLQFYGYSKDEILAMCLTDLYPAD
                     EKKKIVKMLNDLYDYQDAVEWHHLKKDGTMVTVIFRSNDLKYQGHDARVAVITDVTER
                     KLAEEKIKNLNIELEKRVTERTADLVKEIEEREKIALTLEQSRESLRTIIESIPFPVV
                     LVNQDRTIRDANQATLDLLGFNSFDEIVGKNCYEILCIYNSDTCPILSPDQIVDKKET
                     VIINKDKVNIPVLKSAVPIKIEGEEVLLETYVDILKIKIMEMELIQAKEQAQAATQAK
                     SDFLANMSHEIRTPMNAIIGLTYLALQTELDARQHDYITKIKSSAQNLLEIINDILDF
                     SKIEARRLKLENIDFNLEKVFQDTANVVTFKAHQKNLEIIFGIDKNIPRYLVGDPLRL
                     HQILANLTNNAVKFTDTGEIIVRAEMDEEYDDTVKIKFSVKDTGIGLTKEQQDKLFQS
                     FTQADSSTTRRYGGTGLGLAISKQLTELMQGDIWVESVYGKGSTFYFTAVFKKQENQK
                     IEEFVLNPDLRGLKVLVCDDNKSSRLIIKGALETFGFKVVLASSGKAAIEILKKHKSK
                     PFQLIIMDWKMPEMDGIETIQTIRKDPEIPSAPAIIMVSAYSEEKIIDNIEKIGVDAF
                     LLKPITNSTLFDTIMQVFGKTTPRRKRQADRGQRLKSELALIKGARILVAEDNEINQQ
                     VIDGLLTAYGMDVIVAENGRVAVQKCQEMHPSELDLVFMDLEMPVLDGYEATRAIRQI
                     AGYEKLPIVALTADAMSGTKGKVLSAMMNDYITKPIDPNEVYNILVKWIPAKRKKINK
                     GESEQQLAKNLNFPEVSGIDVQSGLRRVAGNQQLYGKILQNFVRENINLITSLQNYLV
                     DNDLEAVEHLVHSLKGSSGNIGAQKLYEVASAFEMELKSKSPDSKNIMELIQQLSMEL
                     ELVFNTIQKIELPAEIISSDKTNKPGKWGIEQKKKCQELKKYLAEYDARAGDIFYLIK
                     SELSQSIPEVDLTKIAESIEQYDYDQALSILEKFIELGDQVNKD"
     gene            complement(6340..6882)
                     /locus_tag="PLI55_06295"
     CDS             complement(6340..6882)
                     /locus_tag="PLI55_06295"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HPB00481.1"
                     /translation="MPHKNKIIVCIVCFLILSFSFIYANLPFNSETEINDLKAAFIYN
                     FTKYITWPDLERNKVFFIGVIGNSDIVNSLRRVAEKKFVKNIPIRVAHYQSVDDLRYC
                     HILFISASEKNQLDPILAKLKKRKTLTIGDTPGFCESGVMINFFMQEDRLKFEMNLAK
                     LESAGLKASSQIKKLARIVE"
     gene            complement(6926..8878)
                     /locus_tag="PLI55_06300"
     CDS             complement(6926..8878)
                     /locus_tag="PLI55_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB00482.1"
                     /translation="MLKLRISVFAVSLFLISVTLAQDSELDEWLEMDLSDLMNIKVTT
                     ATKREQLIDKVPATVRIITEKEIQERAYHSLEDVLKDLPGFQFRNILGFNSYTFQRGA
                     PSQNNLILVMIDGIEINELNSGGFYGGYHYNLQNIKRIEVVYGPSSALYGTNAISGII
                     NLITKDAEDGPRAEASLTAGSFGTLFGDISLRLKPENLPLSVTFSTFYKQTEKADLGG
                     TKGDKNWSENMENFENDYGFDSKIKYKNTIIGLTLQDKQASRTTNYKSLGTNYLDYGT
                     NFHIRFLNAYLSNLYTKKPSWSLKSQIYYRNSTVMDNTIAYITTDTGQVGYYRPNSLI
                     GLEEKFDYEGGSKANLVAGIVMEAEQLASDFSITYSDDPEKAPAKPEEPDYENNTLIS
                     FYVQTQYKFWELVEMTAGARLDQSSYYGTILTPRLGFVYSHKNYTGKLLYTEAFRAPR
                     PWDYNWGIGNPDLKPERMKSIELANNLNFNQRLRCDFSLFHNQIYDMLIEKDSCWVNA
                     ERINTNGLELNLDYRRKKMQLNLNYTFTDSKFSDGKQVPEIARHGFNAGVTYSLLTNL
                     NVNLRGNYLSRRKNPVFISTTKSHYVDPYFVLNSVINYNPIPNLKLQIAVNNLLNARY
                     YHTSNRPPERYRQPQRAFLFKLGYQL"
     gene            complement(9042..10487)
                     /locus_tag="PLI55_06305"
     CDS             complement(9042..10487)
                     /locus_tag="PLI55_06305"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPB00483.1"
                     /translation="MEQLKINQISEQVWEIPPTGAMRVPGRIYANENLMQVIRDDQSL
                     KQVANVAQLPGIVGYSMAMPDIHWGYGFPIGGVAAFDIKEGVISPGGVGYDINCGVRL
                     IRTNLVADQLKIDIKNLIAAIFNSVPSGIGSHGAIRKLSRRELERVAVQGAKWAIEMG
                     YGSADDLQFIEDRGCLADAEPSVISQRAFERGADQLGTLGSGNHFIEIGKVVDIYDST
                     AAQAMGIFKGNLVLILHTGSRGFGYQICDDFIKSTLRATQKYEIKIPDRQLACAPLKS
                     IEGQDYLGAMRAAANFAFANRQVISSLIYDSLLKTLKISPNELGFRPVWDIAHNIAKI
                     EEHQYGGKKIQVCVHRKGATRAFGPGNPELPEIYQAIGQPVLIPGDMGTASYLCVGTE
                     IAMRETFGSACHGAGRILSRHQAKAKARSLDLYQALAESGVYVMAEEKGTIAEEMPFA
                     YKDVGEVVDSVDAAGIVRKVARFKPLGVIKG"
     gene            complement(10497..10940)
                     /locus_tag="PLI55_06310"
     CDS             complement(10497..10940)
                     /locus_tag="PLI55_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HPB00484.1"
                     /translation="MSKSERARKSKFYRIFPHTADLGVEVYGANLLELFEHSALALFD
                     LMANVAKVTEQKTCEFSVEAADIELLLREWLGELLYYSISRQFLFKSCHIKQFGEQTL
                     IASASGEPFNPQKHILKREIKSVTYHELQIKNQGNHWKARFVLDI"
     gene            complement(11162..12001)
                     /locus_tag="PLI55_06315"
     CDS             complement(11162..12001)
                     /locus_tag="PLI55_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPB00485.1"
                     /translation="MLKEDLIYRGKSKDVVEIKSGPYTGKYRLIFTDRATGYLENGKP
                     VFDPGYDNVVGEIPGKGAIACRFATHFFKLLKERGIPSHYIDTVSDNEMIVEPAVPLS
                     MPAESPEFPGAAPLLNLEFTWRNNATGSFWRRYPFVRPCRNIHTIVEAWTKGDSDILI
                     TFEALAETGAMTKDEIKQSIKLVKEIAEIVAQEFASNNLHVIDGKFELGRLKYGDGKI
                     VIIDEISPDVLRVCKGYSPDADGNCTVYKNCTVTSYADGKRTIKNKNQVQAVDFVNIF
                     LSH"
     gene            complement(12226..13251)
                     /locus_tag="PLI55_06320"
     CDS             complement(12226..13251)
                     /locus_tag="PLI55_06320"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HPB00486.1"
                     /translation="MFGLGCLKKNFKRKSLLLFAIVLFLRFQLFSQQFELRDMAKMQW
                     YKGSLHTHAREGESDSSVEYVVKWYREHDYNFLVITDHSTVTIPPAYAIEGDPSFLFI
                     PGEEIMGYGTGRLLEINAFNITEDISPIHSETDLDALQRCLDTVREIGGVPMINHPNY
                     QWRLGADIILNSKNCNLFELYNGCPGTNNYGDDNHPSTEQIWDFMLSAGKRIYATATD
                     DAHVYQKFSAELSNPGRGWVVVKAKKLEVNEILQNLEAGLFYSSTGVEISDLIVKPVQ
                     IEIFIQISGKNQYTTDFIGTKGKLLLRTQNNPAVYKLTSNEKYVRAKIVASDGSCAWI
                     QPIFVVP"
     gene            complement(13352..13424)
                     /locus_tag="PLI55_06325"
     tRNA            complement(13352..13424)
                     /locus_tag="PLI55_06325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13388..13390),aa:Arg,seq:ccg)
     gene            13561..14577
                     /locus_tag="PLI55_06330"
     CDS             13561..14577
                     /locus_tag="PLI55_06330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB00487.1"
                     /translation="MKRILPIILVGFLVFSCAQKEKSAKLEKGTELYQLAQELSQQIP
                     ILDPEKNTVIVTTKKFDITVGDVFTTLHQLYGDGIQQLKSLDTNQLTEIIKGNAIALA
                     EKKLLLQAAEKNGIKISDAEVDSILELQYMQVGGKEKFLNYLSNANINFKALREDIRD
                     NLAINSYLDKMIMDSVNITEEDIQKYYNETDQTATVRHILKITQGKSPEEKAQIHSEM
                     EGILDRAQKGENFESLAKKYSDDPGSKDNGGLYKDFGRGEMVKPFEDAAFDLPIGSIS
                     DIVESPYGYHIIKVIDRKKESRPLDTVRSEIENILINSRQNELLINLLDNLKKENDFK
                     VVIP"
     gene            complement(14650..14988)
                     /locus_tag="PLI55_06335"
     CDS             complement(14650..14988)
                     /locus_tag="PLI55_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB00488.1"
                     /translation="MIIRKVKEVPAEPVAEPGAENVRRQVLLSPSEGAPHFTLRRFEI
                     APGGQTMYHHHNYEHEIYVLAGVGKARGRNSEVRINQDDAILVLPNEEHQIVNTGITP
                     LIFLCLIPNQ"
BASE COUNT         4462 a         3219 c         2705 g         4688 t
ORIGIN      
        1 aaaattctgc catccggagt agaacgatag agcccaatcg ccgcattttc aaaaatggct
       61 ctataatgct cctcactttc ttcaagttca cttacatttt tttccaattt cttgattatg
      121 cgttcactat ataacttgaa agcatctttt tcatcttgaa accttggata ggcgacttcc
      181 agactccctt caattcggtt ttgcagaact tcttgaataa tattcagaaa aaggtccgat
      241 tcaaccggtt tacgtataaa tcgcgaagca cctaatttca gagcgaaaat ctcatcttgt
      301 cgtgaagtaa aagtagccga gtagaaaatt aatggaatgt ccttccagcg cggatcagat
      361 ttaacaatat tgcataacat atagccatcc atctctggca tcaaaatatc cgaaatgatt
      421 aaatcaatgc tattattctc caaaatatcc agcgccaatt tgccgttaac cgcagaaaat
      481 acctggtagt catgacggtt aagaaacgac tctaataggg aacgactctc attattgtca
      541 tcaacaatca acactttcag cataaattgc tcctcctaat tatttttagc attaatgaat
      601 ttaattgtga ttaaagtacc atgatcgtcg gcgtatttct taatatctcg cgactccacc
      661 tcgatttcgc caccataaaa ttcgacgaaa gatttaacta atggcatccc aaaaccggtt
      721 ccaatttcgc ctttagtccc agggcgactt gtaattttag ttatatcaaa aatctggctc
      781 atcaactcag ctggcatgcc aattccatga tcttggattt tgaaaatcac ggcgccatct
      841 tcttctgccg ccgaaatttc gacctgccct ccttcaaagg aaaatttcag ggcattcgtc
      901 atcaaattat taataactga attgattaaa gaacacctct ccgccattac ccataggcta
      961 tggttaatat ctatcaacag tttaacattt ttctgctcaa agcgatcacc aagtagcaaa
     1021 accgattctg ccaaacattc gctcaggttc accgcactta tttctatttt tttctcctgg
     1081 agagcacgca tttctcggac taaattaatg atgtcaatac catgggtagt cgcagttttt
     1141 attttactga ggtaatcggt tatagctgaa tcacttttca gtaaatcgag ggacatactt
     1201 ataacagtaa agtgattggc aaggtcgtga cataaaatat gtaataattc tttctgctcg
     1261 cggttttgtt cgaggatgat atctttttgt tgcttaatta aaagatgggt acggacgcgc
     1321 gccagtagct cgggcggatt aaacggctta gtcagataat caactccacc ggatttaaag
     1381 cctcttacaa tatcatcggt tgccgttctg gcagtcagaa aaataattgg aatatcctga
     1441 gttcgcggat cacgctttag ctcttcacaa acttggaatc catccatacc aggcatcata
     1501 atatcgagca gaatcaaatc gggtcctgct cccattcgag cgattttcag tgctaattga
     1561 ccgttattgg ccgcttttat gtcgtaatca tttattagta gccctttcag aaaattcaga
     1621 ttcgttgcta cgtcatcaac aattaagatt gtaggttttt tattctccat attaatcctt
     1681 attgacctgg tcgcccagtt caatgaattt ctctaaaata cttagggctt gatcataatc
     1741 gtattgctca atagattcgg cgatttttgt caaatctact tcaggaattg attggcttaa
     1801 ttcactcttg atcaaataga aaatgtcacc ggctcttgcg tcatactcag ccaggtattt
     1861 ttttaattcc tggcatttct ttttctgctc aattccccat ttcccgggtt tattggtttt
     1921 atcagaacta ataatttcgg ctggcaattc aattttttga attgtattaa aaaccaactc
     1981 aagttccata ctcaattgct gaattaattc cataatattt ttagaatcag gagattttga
     2041 ttttaattcc atttcaaacg cagaagcgac ttcatataat ttctgggctc cgatattacc
     2101 ggaacttcct ttgagagaat gtaccagatg ttcaactgct tctaagtcat tatctaccaa
     2161 gtaattttga aggcttgtga tcagatttat attttcacgc acaaaatttt gcagaatctt
     2221 tccgtataat tgctggttac cggcgacccg tctcaaacca gactggacat caataccaga
     2281 tacttctgga aaatttagat ttttagccaa ctgctgttca gattcacctt tatttatttt
     2341 ttttctttta gctggaatcc acttaaccag aatgttatag acctcgttcg gatcaatcgg
     2401 tttggtaatg taatcattca tcatcgccga taatactttc ccttttgtgc cggacatagc
     2461 atcggctgtc agcgcgacaa tcggtagctt ttcatatccc gcaatttgac ggatcgccct
     2521 ggtcgcttca taaccatcca ataccggcat ctcaagatcc ataaaaacta aatcgagttc
     2581 agaaggatgc atttcctgac atttctggac ggcgacgcgg ccattttccg ctacgataac
     2641 gtccattcca taagcagtaa gcaatccatc tataacctgc tgattgattt cattatcttc
     2701 ggcaactaag atccgggcgc ctttgataag cgccaactca cttttaagtc tttgccctct
     2761 gtcggcttgg cgtttgcgcc taggagtggt tttgccaaag acctgcataa ttgtatcaaa
     2821 taatgtcgaa ttggtgatcg gttttaataa aaatgcatca actccaattt tttcaatatt
     2881 gtcaatgatt ttttcttcac tataagcgct gaccataatg atggctggtg cagagggaat
     2941 ttcaggatcc tttcggattg tttgaatagt ctcgatacca tccatttcag gcattttcca
     3001 gtccattata ataagctgaa atggtttgct tttgtgtttc tttaaaattt ctatagccgc
     3061 tttgccggaa gaagccagca cgactttaaa accgaaagtc tcaagtgcgc ctttgataat
     3121 taaacgagaa cttttattat cgtcgcaaac cagaactttt aaccctcgta aatcgggatt
     3181 aagaacaaat tcttcgattt tttgattttc ctgcttttta aagacggcgg taaagtagaa
     3241 ggtactgcct tttccataaa cactttcgac ccaaatatcg ccttgcatca gttcggtcaa
     3301 ttgcttagaa atagccaatc ccagaccggt accaccataa cgtctggtag tggaggaatc
     3361 cgcctgggta aaagattgaa agagcttatc ctgctgttcc ttagtaagac caatgccggt
     3421 gtctttaact gagaacttta tttttactgt gtcgtcatat tcctcatcca tttctgcccg
     3481 gacaataatc tcgccggtat cagtgaactt gacggcatta ttagtcaaat tcgccagtat
     3541 ttgatgcaga cgcaagggat cgccaactaa gtagcgcgga atatttttat ctatgccgaa
     3601 gattatttcc agattttttt gatgggcttt aaaggttaca acattggcgg tatcctgaaa
     3661 aactttttcc agattgaaat caatattctc cagttttagt ctacgagctt cgatcttgga
     3721 gaagtccaga atatcattaa tgatttccag caaattctga gcggatgatt ttattttagt
     3781 tatgtaatca tgctgtcgag cgtctaactc ggtttgtaag gctaaatacg ttaaaccgat
     3841 aatcgcattc atcggagttc ttatttcgtg gctcatatta gccagaaagt cacttttggc
     3901 ttgggtagcg gcttgggctt gttccttagc ctgaatcagt tccatttcca taattttgat
     3961 tttgagtata tcaacatagg tttccagcag tacctcttcc ccttcgattt taattggaac
     4021 tgcacttttt agtactggaa tgtttacctt atctttattt atgatcactg tttctttttt
     4081 atctactatc tgatctgggc ttaagattgg gcaagtatcg ctattataaa tacataaaat
     4141 ttcataacaa tttttcccaa caatttcatc gaacgaatta aacccgagta agtcaagagt
     4201 agcctgatta gcatctctga ttgttcgatc ttgattaacc agcactacag gaaatggtat
     4261 ggattcaatg atagttctca gagattctcg ggattgttcc agagtcagtg caattttttc
     4321 ccgttcttca atttctttta ctaaatcggc agttcgttca gtaactcttt tttccagctc
     4381 tatgttcaaa tttttgattt tttcttcggc taattttcgt tcagtaacgt cagtgataac
     4441 tgcgacacgg gcatcatgcc cctgatattt caaatcattc gaacggaaaa tgacggttac
     4501 cattgttcca tcttttttca agtgatgcca ttcgacagca tcttgataat catataaatc
     4561 atttagcatt ttaactattt tctttttttc atctgccgga taaagatccg tcaagcacat
     4621 tgccagaatt tcatctttgg aataaccata aaactgaagg aaagcctcat taaccgctaa
     4681 aatattatag gtattatgtt catagattat gatcggcgcc ggattagatt caaagagcaa
     4741 tcggtaacgg gcttcgcttt cctgaagagc ttgctcagct ttcttccgct cattgatatt
     4801 ttgaaccgcg ccatgaacac caattaatct attgttttta taatcccaaa ccggataagc
     4861 ataaactctt aaccataata gttcaccgga tttagtataa aagcgtaatt cggattggat
     4921 cggttcattc tttttcaatt tttcaaaatc atgatcatct ttttcaagat cgtccggatg
     4981 gagcctggct ctccaaccac cggcttcact aaattccttg aaggtaaatc cagttatggt
     5041 ctcaaaggca ccgccaaccc agtcggttac cagaacacca tcttgattga ttttggccga
     5101 aaaaacataa tccgatacta cggaagatat tcgacgataa cggatttcgc tctgcctcag
     5161 caattcttcg aattccatac gttcagtaat atcagaaacg atgccctgat aatgggtaat
     5221 ttctccggat tcacttctaa tgataaaaga tagatcatca atccacttta ttgcgcctga
     5281 tttagtaaca atccgataag gttcatgttt aaaagattgc acgtcgggtg tttgactata
     5341 cttaactacc tcactattaa ctctcggtaa atcctcagga taaatacatg attcatagga
     5401 tattttgcct tgtataaact catcggcggt atagccaaaa agattaatga cactttcaga
     5461 aacaaactca actggcaagg taggttcgtt ataccaggta aaagctacga ccggactgcg
     5521 attgataatt gtgttagctt ctctgagttt tttttcgact ttatcccttt cttcctgttt
     5581 tttctggatt tgctccagca tttcgttaaa tccatcatag agtattccga tttcatcttg
     5641 agtcgttttt ggtaaacgga ttgaataatc atgtttggtc gtaatttcct tgatagtatc
     5701 aactaatgcc ataatcgggt tggaaacggt cttgccggtg taaaatgaaa gaactaaagc
     5761 tagtccaaaa ccaatcagca aaacgactat tcccagccct aaactctcta atattattga
     5821 ggataaagga tgcatctggt agcgaatcag aatgtagccg accgcaactt cctcttgagt
     5881 aagttctgaa gaataaatta gatagcgaga tttaagctta tatctaccgg aatcaaagaa
     5941 aggataatca taattctcaa acccatcacg cgcaaatgtc gcaaacaaat ttctattatg
     6001 gtcaaataac caggcattaa cgacttcact ttcacttcgt aaggaagcca gaatattttc
     6061 agctgtttcg ttatcaagga ataaaatagc aggaatagaa ttcgagccaa tgatgcgagc
     6121 tgtcgtttct aacttttcat tgacatctcg ctgatactca atctgattta ttattacctg
     6181 aaaaataatg aaaaatacga taacagtgct tactgtaatt aactgaatta caatcagttt
     6241 tttcctaaat gacaacttct gaaaaaatcc ccgattgata gacttcatct tcgccatagc
     6301 atcataccaa ataatttaat ttggctttaa atgaaggcat cattcaacaa tccgggctaa
     6361 ttttttaatt tgggaactgg ctttcaagcc ggcactttcc aatttggcaa gattcatttc
     6421 aaattttaac ctatcttctt gcataaaaaa attaatcatt acaccacttt cacaaaatcc
     6481 gggagtgtca ccgatcgtca aggtcttcct tttcttaagt ttggctaata ttggatcgag
     6541 ttgatttttt tcagatgcag aaatgaataa aatatggcaa tatctcaaat catcaacact
     6601 ttggtagtga gcaactctaa ttggtatatt tttaacgaat tttttctcag ccactcgtcg
     6661 cagagaattg acaatgtcac tattgccaat tactccgata aaaaatactt tattacgttc
     6721 aagatctggc caggtaatat attttgtaaa attataaatg aaagccgctt ttaagtcatt
     6781 aatctcagtt tcactattga atggcaaatt ggcgtagata aaggaaaaac taagaatcag
     6841 gaagcaaaca atacagacga taattttatt tttatgcggc attcaataga tcaattttct
     6901 ttggaataca tcaaatttca tcatatcaaa gttgataccc caatttgaac aaaaaagctc
     6961 gttgtggttg acgatagcgt tctggcggtc ggttactagt atggtagtag cgagcattta
     7021 ataaattgtt gacggcaatc tgcaatttca gatttgggat cgggttatag ttgataacac
     7081 tatttagcac aaagtatgga tcaacataat gacttttagt tgtagaaata aaaacgggat
     7141 ttttacggcg gctgaggtaa ttaccacgca gattgacatt gaggttcgtc aataacgaat
     7201 aggtaacccc agcattgaat ccgtggcggg caatttcagg gacctgcttg ccgtcagaaa
     7261 atttagaatc tgtgaaagta taattcaggt ttagctgcat ttttttccgg cggtaatcaa
     7321 gatttaattc cagaccatta gtatttatgc gttcggcatt tacccaacat gaatctttct
     7381 caattagcat atcataaatt tgattatgga atagcgaaaa atcacagcgt aagcgttgat
     7441 tgaaattcag attattggct aattcaattg atttcattcg ttccggtttc agatccggat
     7501 taccaatgcc ccagttataa tcccagggtc ttggagctcg aaaagcctca gtataaagca
     7561 acttgccggt ataattctta tggctataaa caaaacctag gcgaggagtc aaaatcgtcc
     7621 cataatagga actttgatca aggcgtgctc cagccgtcat ttcgactaat tcccaaaatt
     7681 tgtattgggt ttgaacgtag aaacttatca aagtattatt ttcataatcc ggttcctctg
     7741 gtttagccgg agctttttct gggtcatcac tataggttat agaaaagtcg ctggcgagct
     7801 gttcggcttc cattacaatt ccggcaacca agttggcttt actgccccct tcataatcaa
     7861 atttttcttc cagcccgatt agtgaattcg gacgataata tccaacctga ccagtatcag
     7921 tagtaatata ggcaattgta ttatccatta ccgtcgagtt tctataatat atttgcgact
     7981 tcaatgacca gctcggtttt ttagtgtaca agttgctcaa ataggcattc aaaaagcgaa
     8041 tgtgaaaatt cgtaccatag tcaaggtaat tagtgccgag cgatttataa tttgtcgtgc
     8101 gggaagcctg tttatcctga agtgtcaaac caattatggt gtttttatat ttgattttgg
     8161 aatcaaatcc ataatcattt tcaaaatttt ccatattctc tgaccagttt ttgtcgcctt
     8221 tagtacctcc caaatccgcc ttttcagttt gtttataaaa agtggaaaaa gttacgctga
     8281 gcggcaaatt ttccggcttt agacgcaaac tgatatcgcc gaaaagcgtc ccaaatgaac
     8341 cggctgttag gctcgcttcg gctctcggtc cgtcttccgc atctttggtg attaaattta
     8401 ttattccgga aatggcgttc gttccgtata aggctgatga aggtccataa accacttcta
     8461 ttcgtttgat attctgcaaa ttataatgat aaccgccgta aaatcctccc gaattcaatt
     8521 cattgatctc gatcccatca atcatcacta aaattagatt attctggctg ggcgcgccgc
     8581 gctgaaatgt gtaactgtta aaacctaata tattgcgaaa ttgaaatccg ggcagatctt
     8641 ttagaacatc ttccagactg tggtaggctc gctcttgaat ttccttttcg gtaatgatcc
     8701 gaacggtagc aggcacttta tcaatgagtt gctccctctt ggtagcagtc gtgactttga
     8761 tattcatcaa atcacttaaa tccatttcta gccattcatc taattcagaa tcctgcgcta
     8821 gagtaacact gataagaaat aaagaaacgg caaagacgct aattctcaac tttaacataa
     8881 cagtgccctt ttgagacaat tgttacaaat ttttgagaat aagtcatttt ctttattaaa
     8941 tagccaccaa tcctatatta atcaagaaaa atcgcaatgt caatgcaaat gcgataatca
     9001 ctcgctaaat caacgacttg gtgccattgc gtttatcact tttatccttt tattacgccc
     9061 agcggtttga agcgagcgac tttacgaacg ataccggccg catctaccga atcaaccact
     9121 tcgcccacat ctttatatgc aaatggcatt tcttcggcga tagttccttt ttcttccgcc
     9181 attacataga caccgctttc cgctaatgcc tgatataaat caagactgcg ggctttggct
     9241 ttggcttgat gccgcgataa aattctacca gccccgtggc aggcactacc aaaggtttct
     9301 cgcatggcaa tctctgtccc aacgcataaa taactcgccg tgcccatatc accgggaatt
     9361 agtaccggtt gaccaattgc ttgataaatt tctggtaatt caggattacc gggaccgaag
     9421 gcgcgggttg cacctttgcg atgaacgcaa acctggattt ttttcccgcc atattgatgt
     9481 tcttcaatct tagcgatatt atgcgcaata tcccagactg ggcgaaaacc gagctcgttc
     9541 ggactaattt ttaaggtttt caaaaggcta tcataaatca acgagctaat cacctgacgg
     9601 ttagcgaagg caaaattcgc cgcggctcgc atagctccga gataatcttg cccttctatt
     9661 gattttaatg gggcgcaagc caactgcctg tcaggaattt ttatttcata tttttgggtg
     9721 gcacgtagag ttgatttgat aaaatcatcg caaatttggt aaccgaaacc tcgcgaaccg
     9781 gtatggagaa ttaaaaccag attgccctta aatataccca tcgcctgcgc cgccgttgaa
     9841 tcgtatatat ccactacttt tccaatctcg ataaaatgat taccactgcc gagcgtcccc
     9901 agttgatcgg caccccgctc gaatgcgcgt tgcgatatta cggacggttc ggcatccgca
     9961 agacagcccc tatcttcaat aaactgtaaa tcatcagcac ttccgtaacc catttctatc
    10021 gcccatttag ctccttgaac tgccacccgt tcgagttcac ggcgactgag tttccggata
    10081 gctccatgcg agccaatccc actgggaaca ctattgaaaa tagcggcaat caagttttta
    10141 atgtcaattt tgagctggtc ggcaaccaga tttgtcctta tcaaacgcac cccgcagtta
    10201 atatcatagc cgactccgcc tggagaaatc acaccttctt taatatcaaa agccgctact
    10261 cctccaatcg ggaatccata accccagtga atatcgggca tagccatgga atagcctacg
    10321 attccgggta attgagctac atttgcgacc tgtttaaggc tctgatcatc tcgaataacc
    10381 tgcatcaggt tttcattcgc atagattcgc ccaggaacgc gcattgcgcc ggttggcggt
    10441 atttcccata cttgctcgct tatttgattt atttttaatt gctccattac taacctttaa
    10501 atatcgagga caaagcgcgc tttccagtga ttaccttgat ttttaatttg taattcatga
    10561 taagtaacgc tctttatctc acgttttaaa atatgtttct gcgggttgaa cggttcgcca
    10621 ctcgcgctgg ctattaatgt ttgctcgcca aattgtttga tgtggcaaga tttaaataag
    10681 aattgccggc ttatagaata ataaagcaat tcgccgagcc attcccgcag aagtaattct
    10741 atgtcagcgg cttctactga aaattcgcaa gttttttgct ccgtaacttt cgccacatta
    10801 gccatcaaat cgaacaaagc caaagcggaa tgttcgaaaa gttccagcaa attggcgccg
    10861 tacacctcca ctcccagatc agccgtgtgc ggaaaaatcc ggtaaaattt gctttttcgt
    10921 gcccgttccg atttactcaa atcaacttcc atatttataa ttttaatccc acatttctcc
    10981 ataacctaaa gtaagatata cactattaga gacaagagca aatttgctgt taattaaaat
    11041 taaaaatagg tgattaattc aatagtcgag aatcagagaa ttttatagaa ctgcgcccat
    11101 attctcttat ttcttaaaag agcttactag cacttaaaag aactcaaata cgcttattca
    11161 ctcaatgact gaggaaaata ttgacaaaat caaccgcttg aacctggttt ttattcttga
    11221 tagttctctt tccatcggca taactcgtca cggtacaatt tttataaacc gtgcaattgc
    11281 cgtcagcatc gggagagtag cccttgcaaa cccgcagaac atcgggagag atctcgtcaa
    11341 taattacaat tttcccatcg ccatacttca gtcgccccaa ctcaaatttc ccatcaatga
    11401 catggaggtt gttgctggca aattcctgtg caacgatttc ggcgatctct ttaaccagtt
    11461 taatactctg cttaatctcg tctttagtca tagcaccggt ttccgctaac gcttcaaacg
    11521 taatgagaat atcactatct ccttttgtcc aggcttcaac gatcgtatga atattgcggc
    11581 aaggtcgcac aaaaggatat cttctccaga aactaccagt ggcgttattc cgccaggtaa
    11641 attcaagatt caagagtgga gccgccccgg gaaattccgg tgattcggct ggcatactaa
    11701 gcgggacagc cggttcgacg atcatttcat tatcgctgac ggtatcaata taatgggaag
    11761 gaatacccct ttcctttagc agtttaaaaa aatgcgtggc gaatcggcaa gcaatagcgc
    11821 cttttccagg aatttcgccg acgacgttat catatccagg atcaaaaaca ggtttaccat
    11881 tttccagata acccgtagct ctatcggtaa aaattaaacg atatttacca gtgtatggcc
    11941 cactttttat ttcgacgaca tctttggatt tcccacggta aatcaaatct tctttcagca
    12001 taagtacttt tccttttgta acaattgtaa atcaaaaaca atcaatatca agtttagcaa
    12061 ctgataattt acaacaatcc gaaaaactga ccattaaaat atcaagcctc tgtcaatcca
    12121 ccaaaatttg cccattattt tggataataa ttgcgagcgt cagtatcaat taaactttag
    12181 atctttgctt ttattaataa gccctgccta atttttcagc cgaaattatg gcacaacaaa
    12241 tatcggctga atccaggcac aactcccatc ggaagccaca atttttgccc gtacatattt
    12301 ttcattagaa gtcaatttat agactgccgg attattttgc gtgcgtagca ggagtttgcc
    12361 cttggtaccg ataaaatcag ttgtatattg gtttttgccg cttatctgga taaagatttc
    12421 gatttggaca ggtttgacga ttaaatcaga aatttcgacg ccagtgcttg aataaaataa
    12481 accggcttcg agattctgca aaatttcgtt tacctcaagt tttttagctt tcaccaccac
    12541 ccatccacga cccgggttag ataattcggc agaaaatttt tgatatacgt gagcatcatc
    12601 agtagcagta gcataaattc gctttccagc cgatagcata aaatcccata tctgctccgt
    12661 gcttggatga ttatcatcac cataattgtt ggtaccagga caaccgttat ataactcaaa
    12721 taaattgcag tttttagaat tcaatataat gtctgcgccg agtcgccatt gataattggg
    12781 atgatttatc atcggaacgc ctcctatttc ccgaaccgtg tcaagacatc tttggagagc
    12841 atctaaatcg gtttcgctat gaattggcga aatatcttcg gtaatattaa aggcattaat
    12901 ctccaacaaa cggccagttc cgtatcccat tatttcttcc cccggaataa atagaaagga
    12961 gggatcacct tcaatggcgt aggccggtgg aatagttaca gtcgagtgat cggtaataac
    13021 caaaaagtta taatcgtgtt cccgatacca tttcacaaca tactcaaccg aggaatcgct
    13081 ttcaccttct cgggcgtgag tatgaagact acccttgtac cattgcatct tagccatatc
    13141 gcgtaattca aattgctggc tgaacaactg gaagcgtaag aacagtacta ttgcaaatag
    13201 cagtaatgac tttctcttaa aatttttctt taaacaaccg agaccaaaca cgccaataat
    13261 atatttaaaa ccggcttact ataaaagttt agcttttgga aaaaggttaa tgaacttaca
    13321 atatggcaag atttttttaa cggagactta gtacccccaa caggactcga acctgtgcaa
    13381 ccggctccgg aggccggtgc tctatccatc tgagctatgg gggcttcaat aagcctttta
    13441 agttacatct aacattccaa tttctcaaaa agaattcctt gacaaataat tatgaattaa
    13501 ttcaattttt atctaatttt aatgcgtttt tagaaatatt tctaagtaaa ggagaaaatg
    13561 atgaaaagaa tattgccgat catattagta ggatttttag tcttttcttg tgcccagaaa
    13621 gaaaaaagcg ccaaattaga aaaaggaacc gagctttatc aattagccca ggaactatcc
    13681 cagcaaattc cgatactcga tcctgaaaaa aataccgtaa ttgttactac caaaaaattt
    13741 gatattaccg tcggagatgt tttcaccact ttacatcagt tgtacggaga tggcattcag
    13801 caattaaaaa gccttgatac caatcaattg actgaaataa tcaagggaaa cgctattgcg
    13861 ttggctgaga aaaaactcct cttacaagcg gctgagaaaa atgggataaa aatatcagac
    13921 gccgaggttg acagcatcct tgaattacag tacatgcagg tcggcggtaa ggaaaaattt
    13981 cttaattact taagcaatgc caatattaat tttaaagcct tacgggaaga tatccgtgat
    14041 aatctggcta tcaatagcta tctcgacaaa atgataatgg actcggttaa tattacagaa
    14101 gaagacattc aaaagtatta taacgaaacc gatcaaaccg ctaccgttcg acatatttta
    14161 aaaatcacgc agggaaaatc gcctgaagaa aaagcccaga ttcattccga aatggaagga
    14221 attcttgatc gagcccaaaa aggtgaaaat ttcgaatctc tggctaaaaa atactcggat
    14281 gacccaggct caaaagataa cggcggatta tataaagact tcggccgcgg cgaaatggta
    14341 aaacctttcg aggacgccgc ttttgattta ccaatcggca gtatcagcga tattgtcgaa
    14401 agtccttatg gttaccatat tattaaagtt atcgaccgta aaaaagagag ccgtccgttg
    14461 gataccgtcc ggagtgaaat tgaaaatatt ttaatcaatt cgcgccagaa tgaattactg
    14521 ataaacttgc tggataacct gaaaaaagag aacgacttta aagtcgtgat accataagaa
    14581 tatcaataaa tactttacaa aggagctgaa atttcagctc ctttttattt tatctgctta
    14641 atttaaaagt tactgattcg gaatcaggca caaaaatatc aatggggtaa tacctgtatt
    14701 aacaatctgg tgctcttcat taggcaaaac cagaatcgca tcatcttgat tgattcggac
    14761 ttcggaattc ctcccacgcg ctttaccaac tcccgccaga acatagattt cgtgttcata
    14821 attgtggtga tgatacatag tttgtccacc aggagcaatc tcgaatcggc gcaaggtaaa
    14881 atgtggcgca ccttcactcg gggaaagtag tacctggcga cgaacattct cagcgcccgg
    14941 ttcagcaacc ggttccgctg gtacttcttt tacttttcgg attatcattt tttcatctcc
    15001 tcgataattg ttaaaatttt ttcaataatc gaatcgcaga gaaaccaccc acgcgtcgtc
    15061 agaattaaat gtcc
//
LOCUS       DAOOMU010000053        14990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4240_length_14990_cov_17.711617, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000053 DAOOMU010000000
VERSION     DAOOMU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 14990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14990
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4240_length_14990_cov_17.711617"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLI55_06340"
     CDS             <1..235
                     /locus_tag="PLI55_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPB00489.1"
                     /translation="TSIKYTLPTSAQVEIAIYNIQGQLVRTLSRGEESPGTHIVVWNG
                     KDNIGRNVSSGVYFFQLKTSMGIQEMRKMLLLR"
     gene            362..2311
                     /locus_tag="PLI55_06345"
     CDS             362..2311
                     /locus_tag="PLI55_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HPB00490.1"
                     /translation="MNKPNRFLCLLLLTSIPIWAETQPTFSDYPISAVDIKNVEINDA
                     FWLPKIKIIQDTTIQYAFKKCEEEGRMENFLIAGGRKKGPVRGKMPFDDTDVYKIIEG
                     ASYSLISRPNPALAAYLDSIISIIKIGQEPDGYLTTWFTIDRMHPPAWWAQPSPNRWD
                     HEESNHELYNSGHLFEAAAAHYRATGKQNFLKIALKNADLLVASFGPDKLRVPPGHQI
                     VETGLIKLYHITNNPQYVELARFFLELRGDSTSHKLYGEYSQDHLPVTKQTEAVGHAV
                     RAMYMYAGMTDIAAIYQDSAYFEALMKIWENIVTKKMYLTGGVGARHDGEAFGDNYEL
                     PNLTAYNETCAAIGSVYWNQRLFRLTGDAKYYDIIERTLYNGLISGISLDGKKFFYPN
                     PLESDGKYQFNQGACTRQPWFDCSCCPTNIIRFLPSVPELIYATIADSVYINLYISSN
                     ADIIVNQKKIEIIQQTDYPWDGKINITVNPEKKTIFTLKLRVPGWARNEVVPGDLYKY
                     IAENPEKVSLLVNGKDENGVLDKGYIEITRKWTRGDKIELTLPLAVRKVVAHEKVTDN
                     VNKVAFECGPIVYCAEEIDNHRISEITVPLDTKLNITKGAVLTENVNILRGKVDNQEL
                     ILIPYYVWSNRGVGKMKVWLPINKE"
     gene            2354..3286
                     /locus_tag="PLI55_06350"
     CDS             2354..3286
                     /locus_tag="PLI55_06350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB00491.1"
                     /translation="MIISKPYTGNISRPFVKYPKGASEGRIEIHRPENVTEPLISIII
                     PTMGAARQEFLPGLMSQIERQTFRNIEVILIIGDTRQGRAINCGAALAAGRYLVIFDD
                     DTRLGSDDLLAKMLAQMENHPDIGLAGVANLIPENSNWFVRRVMRELPRRTSPIVSEI
                     TDSDLAEHPCCIIPRDVFIKIGGENELIPRGLDPYLRQEIRQAGYRVVVLPELYIHHL
                     PPSTFSKFVRQFYRNGKMAAYVNKFYPEFVVELAYQHGTEVSQKRSTIVRIGSYVWRL
                     CKTIVTFRAFYLLSLVFYLSGYLIGYITLGEQDA"
     gene            3279..4436
                     /locus_tag="PLI55_06355"
     CDS             3279..4436
                     /locus_tag="PLI55_06355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB00492.1"
                     /translation="MRNQNTLLIPGDFPPVVSGISTYYYQIWRLLPQENNYILCPKDP
                     GWEQFDSQTGLQTIRRRIPAGNLIRDKLLKGLLYAFWTLVLDRKYHFRKIHCGQVLSS
                     GFTGWLMNKLFGIPYNIYVYGSETFRFGYSPLFMKYIKTFLSNAEYIVPNSQFTCNEF
                     MALGIPPEKFKIITPGVDTGFFKPAPKDATLMEKYGLNGKTVLLTVARLDERKGHEVM
                     IRAVADLSSQFPDLIYLIVGAGREEERLRKLTKELNVTDQVIFCGYIPDAELPKFYNL
                     CDIFILLNRQTYDDVRLRGDYEGFGIVFLEAAACGKPVVAGDFGGVASAIENGRSGLI
                     IDANDQSAIRQTLVSLIQNPSLREQLGQYGLERARQSFDWQIISREIMKIL"
     gene            4433..5059
                     /locus_tag="PLI55_06360"
     CDS             4433..5059
                     /locus_tag="PLI55_06360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB00493.1"
                     /translation="MTTLNLKNQTRTAAFQTLTSIRVRTAALKIARQINGRFLDVGCG
                     NGLFLLEYYVTDAKKAIAFGLDHDRQAIYEARTLFKDNNISIEPLLIGDGFNLPFEDE
                     KFEVVFCLNTFLNFHPFEKIEAFIIEMHRICKKGGWIVFDYRNSRNPYLWFRYRLSSL
                     TGRLNVHGHRRKEFQPLMRRLNIKNSEQMAIPSPLPGFPLGYLNKWEK"
     gene            5061..6263
                     /locus_tag="PLI55_06365"
     CDS             5061..6263
                     /locus_tag="PLI55_06365"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB00494.1"
                     /translation="MSILHIPTAGTPYNFTEIARATLGLFESDHVQDNFSQTLKKYFN
                     VENLALVNSGTTALFILLELFKKIRTSPKRNEIILPAYTAPVLLLPIRAAGLNAVLVD
                     VDLTTFNMDVHKILNAVTPQTLAIMPVHMFGLPMDVAPIIESSAGQDIFIVEDAASSL
                     GSTIGGRQTGSIAPFGFYSLNRGKNVSTLAGGIIVWKDQRYSPIIEELVAGLPPVSQR
                     AEFLMWLNFLGLSIAVNPLCYTLLLPFIAKFKYTTLHPHFDSFAYTNMQAALGKTLWR
                     RIDQLTKRRVENGKALMQIFTGKPGYTIPKILPNSQAAFNQFPVIVPNLDQRQRLQKK
                     LLKVGIETTRLYEKPLHHFFPELNPFTYDNYPQATYLAERLLLIPTHAQIGPAIRQKI
                     NSVVEALN"
     gene            6355..>7120
                     /locus_tag="PLI55_06370"
     CDS             6355..>7120
                     /locus_tag="PLI55_06370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB00495.1"
                     /translation="MVKNGYEVLLVAGRTTHPPFDLTEYARLNGFQLYFLRTLVRDPS
                     PIYDPLAFLQIYRLILKYRPEILHTNTSKAGFLGRLAGRLARVPKILHSTHGHIFYGY
                     YNRYISKCFITLEKFSAHYCDRVLNLTEIGRQDHIRERIGPSSKFYVTSGGADLDPFF
                     KACHDNLTLKSPDEKIRICWVGRIEPVKNLPLLLRAAGILEHSGLNLEYIVVGDGADR
                     QYNEKLVADLGLKNIQFLGYRTDVPEIMASSDIFVFT"
     assembly_gap    7121..7220
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7221..7574
                     /locus_tag="PLI55_06375"
     CDS             <7221..7574
                     /locus_tag="PLI55_06375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB00496.1"
                     /translation="YRTDVPEIMASSDIFVFTSLNEGFGNVIIEAMACGLPIIGTRVG
                     GVPSLIQDGVNGLLVNSDDAEALATAIQIIASNPEQRQSMASTNRKKAEYFTIDNYIK
                     RVIYAYQTCNTNLPE"
     gene            7537..9114
                     /locus_tag="PLI55_06380"
     CDS             7537..9114
                     /locus_tag="PLI55_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00497.1"
                     /translation="MLIKRVTRICLNKKFAPFLITISLLIMTDSLGFCQTKPGYILLK
                     AGVHFDSQVSGGKYSLAELANIIARHKLDVGIITDHDNMKVSYGIRPFENFLKFTIEE
                     NSVHRYGVEKYINEINALDAAYPNLILIPGIEAVPYYRWEGSPLNENMVLRDWHTHLL
                     VFGLEDPKVFEQLPSLANHNIGYSKPDGKIIKYIGENFTHFALMTLYFLLFLISLALI
                     FRRKYGLFSRKNINRPRQNIRFSFPAFIMAIIFGLILYNQYPFLPRKYTPYDRKAGSA
                     PYQELIDYVNAHNGLIFWAHPEVTHNEVRQVNIPLMNQAIKISTEAYPHRIMETQDYT
                     GFAIFWEGAKVIGRPGGLWDIALAEYCAGLRRKPVWAIGELDFEDSSNLNNVTETLTF
                     LFVKERSRAGVYEAMRAGRMYATRSFTGDKIVIDDFSVYDLHTGNNAFIGETLTHAQA
                     PVAIHIRLRALTTLNKPETIFLYRNSELIKIFHLNTVLDEWFVDESPMPNPMYYYRIY
                     GGDSWPTLASNPIFVSK"
     gene            complement(9126..9311)
                     /locus_tag="PLI55_06385"
     CDS             complement(9126..9311)
                     /locus_tag="PLI55_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00498.1"
                     /translation="MDTIIKGCIIVGVISLILGIVSRLTLKPFFVEAQAFMQFAQFCF
                     LAAITFLLYKIAYKVKD"
     gene            9379..10077
                     /locus_tag="PLI55_06390"
     CDS             9379..10077
                     /locus_tag="PLI55_06390"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HPB00499.1"
                     /translation="MICAIHQPQTYPWLGYFAKIMQADIFIFLDNVQFKKNEWQNRNK
                     IRTPQGWIWLTVPVIHHFGQKIDEVKINNTVNWRKKHLQTLQTYYGRAPFFKLYFEKL
                     ENLYQFEWQNLAQFNITAIRLLMSLIGIDTPTVIASEIKALNQNSQISAAERLIIATK
                     AVGADCYLSGIGGCNYLQPELFAKNDIKLKFQSFEHPVYRQMTNDFLPNMSILDLLFN
                     EGQRANSIIQGGIR"
     gene            10074..10712
                     /locus_tag="PLI55_06395"
     CDS             10074..10712
                     /locus_tag="PLI55_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPB00500.1"
                     /translation="MSSSEKKMNIIAIGSHPDDIEIGCGASLAKFNAKGHSVYLYVAT
                     LGGAGGDPEVRRTEQENSARIMGVKDIFWGSFEDTDITINKNSIAALEEIIHKVTPDM
                     ILVNYHDDTHQDHRNIAAITNSATRYIPNVLFYETPTTQNFMPNVFVDIGTEYLAIKL
                     RALLAHTSQINRTNIANLSIIQVAESSAHFRGVQARVACAEAFHSLRLFIDI"
     gene            10709..11752
                     /locus_tag="PLI55_06400"
     CDS             10709..11752
                     /locus_tag="PLI55_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB00501.1"
                     /translation="MIPHYRPTFGQEEITAVTSVIRSGQLAQNGQVNLLEQILAENLD
                     RKYACAVSSGTMALILSLKASGIGPGDEVIIPSYTCAALWQAVTTVGATAIYADIEDE
                     TYNLAPPSVKLKMTPRTKAIIFPHMFGQPGHIQEVIEYGIPVIEDIAQAYGATIDEQP
                     AGHFGDIGVLSFYATKVIGAGEGGAILTDSPEILEKVRDLRGYDEKDDLKPRFNAKMT
                     DLGAAIAIEQIKKYQSYFTKRTAIIQQYQEVDGIEFVLPLKNSRLKSNNYRCIAAHPH
                     KSAAECIALAAKLGACFRKPVYKPLHSYAQTESLPETEKAWKKQFSIPVFPTISGTDR
                     DRVTEILTHIAEL"
     gene            11749..12915
                     /locus_tag="PLI55_06405"
     CDS             11749..12915
                     /locus_tag="PLI55_06405"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPB00502.1"
                     /translation="MNNHNKKILFRMLVLPFLMALLLPYVAKTWMFDHNLSKIYYLLV
                     TSLMTFGLIPIFYKLGVIMDITDKPGGRHIHQHATPRTGGIAIFITFIITLNIVTKPP
                     REFTGLFLASSVVAFVGIIDDIKHVPALIKLGAQILATIILIKYGIYLSFLPNHIIGN
                     IFSYVLTFIWIIGITNAYNCLDGLDGLAAGAAIIILLFYIIIATYMVADEFMAIVGLI
                     LLGAIIGFMPYNFRITKNALIFLGDAGSTFIGFTLAALSIYGNWGISKSVDLVIPILL
                     LGVPIADMIMTTITRIKDGKVKSVRQWLEYGGNDHFHHRLLQMGFTKTGSVLIIWAIT
                     TVLGLLSLLLKQGDRPEAIIAILIALILFALIISLMLFEYRQERRSLSNKKNIP"
     gene            12944..13741
                     /locus_tag="PLI55_06410"
     CDS             12944..13741
                     /locus_tag="PLI55_06410"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB00503.1"
                     /translation="MYLDYWKIKEKPFENSPDPKFLFHSKQHDEALFRLLYAIQSSRG
                     AALLTGEYGCGKTLLMHTIISELSQGKFKIAYLTNPRWSAMELIEEILYQLEINSKSK
                     SRLEMLHSLNDFLFQNVQEKCQTIIIVDEAQVISDYETFEELRLLLNFQLNDRFLLTL
                     FLVGQPELNNMIKNIPQLNQRIAVRFHLKHFNFEETKAYIQYRLKVAGRNDQIFTDSA
                     FDEIYKYSEGIPRIINNICDLSLVIGFGKHLSTIDHNVIKGIINSER"
     gene            13747..14925
                     /locus_tag="PLI55_06415"
     CDS             13747..14925
                     /locus_tag="PLI55_06415"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPB00504.1"
                     /translation="MAGMQDILKNSQESNNQNNSQKGKNEKNDNNFVGLSEMEAVKKA
                     ITENLGSTTNTFNSAADSTPVDFEPDLVLTPADFPKNGFFKDSHLDEKKLKEIQCQDE
                     IAANQLYMDLLHELGNVFQSLKDNKPLDPQPLEKFIDLMHNSITSGDFLLLKAIQHKR
                     YAAWLISHSANVTILAYKIGLDLKYDRPTLHKLGISALLHDIGMLKVPNNIIFKHGQL
                     TPDEFEIIKKHPLDGFEMAKHLEQYPHIIEVILQEHEREDGSGYPQGLPGDEIAEFAK
                     IVGLADVFEAMVHGRAYREGFITYTAIQKIIEKRSKQFNPRIIRALINSVPMFPVGSL
                     VKLSTNEIARVLSVNSLRPVRPVVEILIDSDGNRLKNPIRLDLEAEPLIYIAKPLAES
                     "
BASE COUNT         4333 a         3336 c         3005 g         4216 t
ORIGIN      
        1 cacttctatc aaatacactc ttcctacgag cgctcaggtt gaaattgcca tttacaatat
       61 tcagggacaa ttagtgcgga cgctttccag aggagaagaa tcgccaggaa ctcacatcgt
      121 agtctggaat ggaaaggata atatcggtag gaatgtcagc agtggtgtct atttctttca
      181 gctgaagacg tcaatgggca ttcaggaaat gaggaaaatg cttctcctca gatgatctgc
      241 taaccgttac ctaacggctg ataataaaga ctctccagtg cgagtcattt caattctcat
      301 attctgagtt tatgagatat ttttacaata aatttacacg taattaatgt gaggtaatac
      361 aatgaataag ccaaaccgat tcctttgtct attactgtta acttcgatcc cgatttgggc
      421 tgaaacacag ccgacttttt ccgattatcc tatatcagct gtggacatta aaaacgtgga
      481 aattaacgac gccttctggt tgccgaagat caaaatcatt caggacacta ccattcaata
      541 cgcttttaaa aagtgcgaag aagaaggtcg gatggaaaat ttcctgattg ctggtggtcg
      601 caaaaaaggc ccagtcagag gtaaaatgcc ctttgacgat accgacgtct ataaaatcat
      661 cgagggcgca tcctattcat taataagccg acctaatccc gctctcgcag cttatctgga
      721 ttccattatt tcgataatca aaatcggaca ggagcccgac ggttatctca ctacctggtt
      781 caccattgac cgtatgcatc caccggcgtg gtgggctcag ccatcgccga atcgctggga
      841 tcacgaggaa tccaatcatg aactttataa cagcggccat ttattcgaag ccgccgccgc
      901 tcactaccgg gctaccggca agcaaaattt cctaaaaatc gccttgaaaa acgctgatct
      961 gctggttgcc agcttcggac cggataaatt acgcgttccg cccgggcatc agattgtcga
     1021 aaccgggtta atcaaactct atcatatcac taacaatccc caatacgttg agttagctcg
     1081 cttttttcta gaactgcgcg gcgatagcac ttctcataaa ttgtacggag aatacagtca
     1141 ggatcatttg ccggttacca aacaaaccga agccgtcggg catgccgttc gggctatgta
     1201 tatgtatgcc ggaatgaccg acattgcggc tatttatcaa gattcggctt atttcgaggc
     1261 tttgatgaaa atctgggaaa atatcgtcac taaaaaaatg tatttaaccg gcggcgtggg
     1321 cgcaagacac gatggcgagg ctttcggtga taattacgaa ctgcctaatc tcactgctta
     1381 taacgagacc tgtgccgcca tcggtagcgt ttactggaat cagcgcctct ttcggctcac
     1441 cggcgatgcg aaatattatg acattatcga acgcacactg tataacggac tcatttcagg
     1501 aatttcattg gatgggaaaa aattcttcta ccccaatcca ctcgaatcgg atggaaaata
     1561 tcaattcaac cagggagctt gcacgcgtca accctggttc gactgttcct gttgcccgac
     1621 caatattatc cgcttcctcc cttctgtacc cgaactgatt tatgccacga ttgctgacag
     1681 tgtttatatt aatctctaca tttccagcaa tgccgatata attgtcaacc agaaaaaaat
     1741 cgagataatc cagcagaccg attatccctg ggatggcaaa attaatatta cagtcaatcc
     1801 ggaaaagaag accattttca ccttaaaatt acgtgtgcca ggctgggctc gtaacgaagt
     1861 ggtcccgggt gatttatata aatacattgc tgaaaatcct gaaaaagtca gtttgctcgt
     1921 aaacgggaaa gacgaaaatg gcgtgcttga taaaggttac atcgaaataa ccaggaaatg
     1981 gactcgtggt gataaaatag aactaaccct gccgctggct gtccgtaaag tcgttgctca
     2041 tgaaaaggta actgacaatg tcaataaagt tgctttcgaa tgcggtccaa tcgtctattg
     2101 tgccgaagaa attgataacc accgaatctc agagataacc gtaccactcg atactaaatt
     2161 aaatatcaca aaaggcgcag ttctaactga aaatgtcaat attttaagag gcaaggttga
     2221 caatcaagaa ttaatcctga ttccctatta tgtctggtca aaccggggag tcggaaaaat
     2281 gaaagtatgg ttaccaatca ataaagaata atagcaaata ttttcatcaa atatctctaa
     2341 cttattaaat attgtgatta taagtaaacc ttatactgga aatatttccc gcccgttcgt
     2401 taaatatccg aagggcgcat ccgaagggcg catcgaaatt caccgcccgg aaaatgtcac
     2461 cgagcctttg atcagcatca ttatccctac aatgggtgcc gctcgacagg aatttttgcc
     2521 cgggttgatg agccaaatcg aacgccagac tttccgcaat attgaggtaa ttttgataat
     2581 cggcgatacc cgtcaaggac gggcaatcaa ttgtggtgcg gctttggcgg caggcagata
     2641 cctggtcatt ttcgacgacg acacgcgcct gggctctgac gatttattag caaaaatgct
     2701 ggctcagatg gaaaatcatc cggacatcgg tctggctgga gtcgccaatc ttattccgga
     2761 aaattccaac tggtttgtac ggcgagtaat gcgcgaactg ccccgccgca cttcaccgat
     2821 cgtctccgaa attaccgaca gcgatttggc tgaacatccc tgttgcatta ttccgcgtga
     2881 tgttttcatt aaaattggcg gcgaaaacga attaattccg cgcggactcg atccttatct
     2941 gcgccaggaa attcggcaag ccggttatcg tgtggtagta ttaccggaac tttacattca
     3001 ccatttgccg ccgtcgactt tctccaaatt cgtgcggcaa ttttatcgca acggcaaaat
     3061 ggcggcttac gtcaataaat tttacccaga attcgtcgtc gaattagcct atcagcatgg
     3121 aaccgaggta agccagaaac gatctacaat tgttcggatc ggcagttacg tttggcgtct
     3181 atgtaaaact attgtaactt tccgagcatt ttatttactg agtttggtat tttatttgag
     3241 tggttatcta attggttata taaccttggg ggaacaagat gcgtaatcaa aacacccttt
     3301 taatccccgg cgattttccg ccggtagtta gtggtatctc aacctattat tatcaaatct
     3361 ggcgacttct tccgcaggaa aacaactata tcctttgccc caaagatccc ggctgggaac
     3421 agttcgactc tcaaaccgga ctgcaaacta ttcggcgacg tattccagcc ggcaacttaa
     3481 tccgcgataa acttctaaaa ggtttgctat acgcattctg gacattggtt ctggatcgca
     3541 aatatcattt ccgtaaaatc cactgcgggc aggtattgtc gtctggattt accggctggt
     3601 taatgaacaa gttattcgga atcccataca atatttacgt ttatggctcc gaaactttcc
     3661 gcttcgggta cagtcctctt ttcatgaaat acataaaaac tttcctatcc aacgcagaat
     3721 atatcgtccc gaacagccaa tttacctgta atgaatttat ggcgttaggc attcctcctg
     3781 aaaaattcaa aataattacc ccaggcgtgg acaccggttt tttcaaacca gctccaaaag
     3841 atgcgacttt aatggaaaaa tacggtctaa acggcaaaac tgtgcttttg acagttgccc
     3901 ggctcgatga acgcaaaggt cacgaagtga tgattcgggc tgtagcggac ttatcatcgc
     3961 aatttccaga tctcatttac cttatcgttg gcgccggacg cgaagaagaa cgtctccgaa
     4021 agttgaccaa agaattgaat gtaactgacc aggtgatttt ttgcggttat atacctgatg
     4081 ccgaattacc aaaattttac aatctatgtg atatttttat cctattaaat cgccagactt
     4141 atgacgacgt ccgcttacga ggcgattacg aagggttcgg catcgttttt cttgaagcgg
     4201 cggcttgcgg gaaaccggtt gtcgccggtg atttcggcgg ggtcgcgtct gctatcgaaa
     4261 acgggcgctc cggcctcata attgatgcca acgatcaatc cgccattcga caaactcttg
     4321 tatctctcat ccaaaatcca tctctaagag aacaattagg gcaatatgga ctggaaagag
     4381 ctcgtcaatc ttttgattgg caaatcatca gccgagaaat aatgaaaatt ttatgacgac
     4441 tttaaatctc aaaaatcaga cccgtaccgc ggctttccaa accctgactt ccatccgcgt
     4501 gcgaaccgcg gctcttaaaa ttgcccgcca aatcaatgga cgttttttgg atgtgggttg
     4561 cggcaacggt ctgtttctac ttgaatatta tgtgactgac gcaaaaaagg caattgcgtt
     4621 tggactcgat cacgaccgcc aggcaattta tgaagctcga acacttttta aagataataa
     4681 tatttctatc gaacctcttc taatcggcga cggttttaac ttaccttttg aagatgaaaa
     4741 atttgaagtc gtattttgtc tgaatacctt tcttaatttt catcctttcg aaaagattga
     4801 agcatttatt attgaaatgc atcgcatctg taaaaaaggc ggctggatag tattcgatta
     4861 tcgaaacagt cgtaatccat atttatggtt cagatatcgg ctcagttctc tgacaggacg
     4921 cctgaacgtt cacggtcatc gccggaaaga atttcaaccc ctaatgcgaa gactaaatat
     4981 taaaaatagt gaacaaatgg cgattccctc accgctacct ggttttccac tgggctactt
     5041 aaacaaatgg gagaaatgaa ttgagtattt tgcatatacc gactgccgga acaccctata
     5101 atttcaccga aatagcgcgt gctacgctgg gtttgtttga gagcgaccat gtccaggata
     5161 atttctctca aacattaaaa aaatatttca atgttgaaaa tctagctttg gtcaattctg
     5221 gcactacggc acttttcata ttgttggagt tattcaagaa gatccgcact tccccaaaac
     5281 gtaatgaaat aatcttaccg gcttatactg cgcctgtttt actcttacca attcgtgccg
     5341 ctggcctgaa tgccgtttta gttgatgttg acctgactac tttcaatatg gacgtgcaca
     5401 aaatcttgaa tgccgtaact cctcaaacgc tggctattat gccggtgcat atgtttggtc
     5461 tgccgatgga tgtagctccg atcattgaat ccagcgcagg acaggatatt tttattgtcg
     5521 aggacgcggc ttcttcgctc ggttcaacca taggtggtcg ccagaccggc tcaatcgcgc
     5581 cattcggatt ttattcgctg aatcggggca aaaacgtctc gactttagcc ggtggcatta
     5641 ttgtctggaa agatcaacgc tattcaccaa ttatcgaaga attagtcgcc ggattaccac
     5701 cagttagcca aagagccgaa ttcctaatgt ggctgaattt tctcggactt tccatcgctg
     5761 taaatccatt atgttacact ctgctgttgc cttttattgc caaattcaaa tatacgacct
     5821 tgcaccccca ctttgattct tttgcttaca ctaatatgca agccgctctg ggaaaaactc
     5881 tctggcggcg tatagatcaa ttgaccaaaa gacgcgtcga aaatggcaaa gcgctgatgc
     5941 agattttcac cgggaaacca ggctacacca taccgaaaat tttgcccaac tcgcaagcgg
     6001 cgttcaacca atttccggtc attgttccaa atctggatca gcgccagcgt ttacagaaaa
     6061 aattgcttaa ggttggcata gaaaccactc gtttatacga gaaaccactg caccactttt
     6121 tcccggaatt aaatccattt acttatgata attatccgca ggcaacttac ctggctgaac
     6181 gtttacttct aattccaacc catgctcaaa tcggtcccgc aattcggcaa aaaattaatt
     6241 cggtcgtaga ggcgttaaat taacagaaaa ccccgtcagc caagagttct acaattaata
     6301 acccgtcttg atcaaggcgg ttcgtctaat gtcactctga agttatgcgc cggtctggta
     6361 aaaaacggct atgaagttct gctggtagct ggtagaacta ctcatcctcc gttcgatcta
     6421 acagaatacg cccgcctaaa tggatttcag ctgtattttc tgagaacatt agtgcgtgat
     6481 ccttcaccta tttatgatcc cctggctttt cttcagattt accgcttgat cttaaaatac
     6541 cgaccggaaa ttttacacac caatacctcc aaggctgggt ttctggggcg tttagccgga
     6601 cgcttagcta gagtgccaaa aattttgcat tcgacccatg gccacatttt ttatggctat
     6661 tacaaccggt atatttcaaa atgttttata accttagaaa aattttcggc acattactgc
     6721 gatcgagtat taaacttaac cgaaattggt cgccaggatc atattcgcga acgcattggt
     6781 ccatcctcga aattctacgt cacttccggc ggagctgatt tggacccatt ctttaaagcc
     6841 tgccatgata atttaacact gaaatcaccc gacgaaaaga tacgcatctg ttgggttggc
     6901 cgcattgaac cggtcaaaaa cctgccgtta ctgctcagag cggctggcat tttagagcat
     6961 agcggcttaa atctggaata tatcgtcgtc ggcgatggcg cagaccggca gtataacgaa
     7021 aaactggtcg ccgatcttgg tttgaaaaat atccaatttc tcggctaccg caccgatgtt
     7081 ccagaaatta tggcatcgtc ggatatcttc gtctttacct nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn taccgcaccg atgttccaga aattatggca tcgtcggata
     7261 tcttcgtctt tacctcgcta aacgagggct tcggcaatgt gattatcgag gcgatggctt
     7321 gcggcttacc gattatcgga acgcgcgtcg gcggcgtccc atcattaatt caagatggtg
     7381 taaatggttt gctggttaat tcggatgatg ccgaagcttt agccacagcc attcagatta
     7441 tcgccagcaa tcctgagcaa cgacaatcta tggcttctac aaaccgaaaa aaggctgaat
     7501 atttcacgat tgataattat attaagcgag ttatttatgc ttatcaaacg tgtaacacga
     7561 atctgcctga ataagaaatt cgcacctttt ttaataacga tcagtttact aataatgact
     7621 gactctctcg gcttttgtca gactaagccc ggttatattt tactaaaggc cggtgtgcat
     7681 ttcgactcgc aagttagcgg cggtaagtat tctctggcgg aactggctaa tataatcgct
     7741 cgccacaaat tagatgtcgg tatcataacc gaccatgata atatgaaagt ctcttacggc
     7801 attcgaccct ttgaaaattt tttgaaattt acgattgaag aaaattccgt tcaccgctac
     7861 ggagtggaga aatatatcaa cgaaatcaac gctctcgacg cggcttatcc taacttgatt
     7921 ttaattccgg gcattgaagc tgttccttac tatcgctggg aaggttcgcc gcttaacgag
     7981 aatatggttc taagagattg gcatactcac ttactcgttt tcggtctcga agatccaaaa
     8041 gttttcgaac aattaccttc gctggctaat cacaatatcg gttatagcaa acccgatggt
     8101 aaaattatta agtatatcgg cgaaaatttt actcactttg cgttgatgac actctatttt
     8161 ttattatttc taatatcatt agcgttaatt ttccgacgaa aatatggatt atttagccgt
     8221 aagaatatca atcgtccgcg ccaaaatatt cgcttttcat ttccggcttt tattatggcg
     8281 attatatttg gactgatttt atataatcaa tatccgttcc tgccgcgtaa atacacaccc
     8341 tacgaccgca aagctggcag tgctccttat caggagctaa ttgattacgt caatgcccat
     8401 aacggtctga ttttctgggc tcatccggaa gttacccata atgaagttcg gcaggttaat
     8461 atccccctaa tgaatcaagc cattaaaatt tccaccgaag cttatcctca tcgcattatg
     8521 gaaacccagg attataccgg atttgccatt ttctgggaag gggcgaaagt catcggccga
     8581 ccgggcggtt tgtgggacat agcattggct gaatattgcg caggcttacg tcgcaaacca
     8641 gtctgggcaa tcggcgaact cgatttcgag gattccagca accttaacaa tgttactgaa
     8701 accttaactt tcctatttgt taaagagcgt tcgcgagctg gtgtttatga agctatgcga
     8761 gctggacgga tgtatgcaac gcgcagtttc accggcgata aaatcgtcat tgatgatttt
     8821 tctgtttatg atctgcacac cggcaataat gccttcatcg gagaaacatt gactcatgcg
     8881 caagcgccgg ttgccatcca tattcgattg cgagcactaa ctacgctgaa taaaccggaa
     8941 acaatttttc tctatcgcaa tagcgaattg atcaaaattt tccatttgaa tactgtttta
     9001 gatgaatggt tcgtagatga gtcgccgatg ccgaatccga tgtactatta ccggatttat
     9061 ggcggcgaca gctggccaac tctcgccagc aacccgattt ttgttagtaa ataacggtag
     9121 gagtgttagt ccttaacctt ataggctatt ttataaagta aaaaagtgat tgcggctaag
     9181 aaacaaaact gcgcgaactg cataaatgcc tgcgcttcaa caaaaaaggg cttcaaagta
     9241 agacgtgaaa ctatgcctag gattaaggaa atgactccga cgataatgca acctttaatg
     9301 attgtatcca ttgtagctcc taattttttc atcattggca aactaaggta aaaatgggat
     9361 aaaaatcaag gaaatttaat gatctgtgcc attcatcaac cgcaaactta tccgtggctg
     9421 ggttattttg ccaaaattat gcaggctgac attttcattt ttctggacaa tgtccagttc
     9481 aaaaaaaatg aatggcaaaa tcgcaataaa attcgcactc cgcaaggctg gatttggctg
     9541 acggttcctg taattcatca ctttggtcaa aaaatagacg aggtcaaaat caacaacacg
     9601 gtcaattggc gcaaaaaaca tcttcaaact ctacaaactt actatggtcg agcaccattt
     9661 ttcaagttat acttcgagaa gctcgaaaat ctttatcaat tcgagtggca aaatctcgcc
     9721 cagttcaata taactgcaat tcgcttatta atgtctttaa tcggaattga tactccgaca
     9781 gtaattgctt cggaaataaa agctctcaac caaaattccc agatcagtgc cgccgaacga
     9841 ctgataattg ctaccaaagc cgtcggggct gattgctatc tctcaggaat cggtggctgt
     9901 aattacctac aacctgagct ttttgcaaag aatgacatca aacttaagtt ccaatcgttc
     9961 gagcatcccg tttatcggca aatgactaac gatttcctac caaatatgtc aattctcgat
    10021 ttattattta atgaaggtca acgagccaat tctattatcc aagggggaat tcgatgagct
    10081 catcagaaaa aaagatgaat atcatagcca tcggctctca tccggatgac attgaaatcg
    10141 gatgtggtgc cagcttagcc aaattcaatg ccaaaggaca ttcagtttac ctgtatgtag
    10201 ccactctggg aggcgcaggc ggcgatcccg aagtgcgccg gaccgaacaa gaaaattcag
    10261 cccgcataat gggcgttaaa gacattttct ggggtagttt tgaagataca gatataacaa
    10321 tcaataaaaa ttccatagcc gccctcgagg aaatcattca taaagtgact cccgatatga
    10381 ttctggttaa ttatcacgat gacacccatc aggatcaccg caatatagcc gcgatcacca
    10441 attccgccac gcgctatatt cccaacgtac ttttttacga aacccccaca actcaaaatt
    10501 ttatgccgaa cgttttcgta gatatcggca cagaatattt ggcaatcaaa ttaagagctt
    10561 tgttggctca tacttctcaa atcaatcgca caaatatcgc taatctttcg attattcaag
    10621 ttgccgaatc cagtgctcac tttcgaggcg tgcaggcacg agtcgcctgc gccgaagctt
    10681 ttcattcact ccgtttattt atagatatat gattccccac tatcgcccaa cctttggtca
    10741 agaagaaatc accgccgtga catctgtcat ccggagcggc cagcttgccc aaaatggtca
    10801 ggtgaatcta ctggagcaaa ttttggcgga aaatctcgat cgaaaatatg cctgtgcagt
    10861 atcttcagga actatggctt tgattttgtc cctaaaagca tcgggaatcg gaccaggcga
    10921 tgaggttatt attccatcct atacttgtgc ggctttgtgg caagcagtca ctactgttgg
    10981 tgcgaccgca atttatgccg atatagaaga tgagacttat aatctggcac cgccatcagt
    11041 caaattaaaa atgacacctc gcacaaaagc gatcattttc ccacatatgt ttggccaacc
    11101 cggtcatatc caagaggtca ttgaatatgg cattccagta atcgaagaca tcgcccaggc
    11161 ttatggtgct actatagacg aacagccagc tggtcatttt ggagacattg gcgtactctc
    11221 tttttatgcg accaaagtca tcggggcggg agaaggcgga gctatcctta ccgattcgcc
    11281 agaaattttg gaaaaagtcc gtgacttgcg tggatatgat gaaaaagacg atttgaagcc
    11341 gcgctttaat gctaaaatga ccgatttggg tgcggctatt gcaattgaac aaatcaaaaa
    11401 gtaccaatcc tatttcacca aacgcacagc tattattcag caataccaag aagttgacgg
    11461 aatcgaattt gtactaccct taaaaaattc gcggcttaaa tcgaacaatt atcgctgtat
    11521 tgccgctcat cctcataaat cggccgctga atgcattgct ttagccgcta agttaggggc
    11581 ttgtttccgg aaaccggttt ataagccact ccacagctat gctcaaactg aatcgctccc
    11641 cgaaaccgaa aaagcctgga aaaagcaatt ctcaattcca gtgtttccga caatttcggg
    11701 tacagaccgt gatcgagtca cagaaatctt aactcacata gcagaactat gaacaatcac
    11761 aataaaaaga ttctctttcg gatgctcgtt ttaccgtttt taatggcact tctattaccc
    11821 tatgtggcta aaacctggat gttcgatcat aatttatcga aaatctatta tcttttagtt
    11881 accagtttga tgactttcgg tctaataccc attttctaca agctcggtgt aattatggat
    11941 ataaccgata aaccgggtgg tcggcacatt catcagcatg ccacccctcg gaccggtggc
    12001 attgccatct ttattacctt tatcattacg cttaatattg tcactaaacc gccgcgcgag
    12061 tttaccgggc tttttttggc tagcagtgta gtagcttttg ttggtattat tgacgatatt
    12121 aaacacgttc ccgcactgat aaaacttggt gcccagattc tggctacgat aattttaatc
    12181 aaatatggga tttatttgtc cttccttcct aatcatataa ttggcaatat tttttcctat
    12241 gtattgactt ttatctggat tatcggcatc actaacgcct ataactgcct cgacggatta
    12301 gacggcttag ccgctggagc tgctataata atcttgcttt tttacattat catcgctact
    12361 tatatggttg ccgatgaatt tatggctata gttgggttga tattattggg tgcaattatc
    12421 ggattcatgc catataattt cagaattaca aaaaacgcct taattttctt aggagatgcc
    12481 ggaagcacct ttatcggatt tacattagcg gctttgagta tttatggaaa ttgggggatt
    12541 agtaagagcg ttgatctggt gattccaatt ctcctcctcg gtgtaccgat tgccgatatg
    12601 ataatgacta ctattactcg gattaaagat ggcaaagtca aatccgtccg ccaatggctt
    12661 gaatacggtg gcaatgatca ttttcatcac cgactcttac agatgggatt caccaaaact
    12721 ggtagtgtcc taataatctg ggcaattacc accgtattgg gactgttatc gttactcttg
    12781 aaacaaggtg atcgcccaga agccatcatt gctatcctga ttgccttaat attattcgcc
    12841 ctaattattt cacttatgct atttgaatat cgccaagaac gtcgcagttt atcaaataaa
    12901 aaaaatattc cttaaatgaa tttattagct tacatttaga tatatgtatc tggattattg
    12961 gaaaataaag gaaaagcctt tcgaaaactc accggacccg aaattcttat ttcattccaa
    13021 acaacacgat gaggctctat ttcgtttact atatgccatc cagagttcaa gaggggcagc
    13081 actcctgacc ggtgaatatg gttgcggcaa gactttatta atgcacacga tcatctccga
    13141 actctcgcag ggaaagttta aaattgctta tctgaccaat ccgcgctgga gtgctatgga
    13201 attaattgag gaaattcttt atcagctgga gattaattcc aaatccaaat cgcgcctcga
    13261 aatgctgcat agtctcaatg actttctgtt ccaaaatgtc caagaaaaat gtcaaacaat
    13321 cattatcgta gatgaagccc aggtaatcag cgattatgaa acatttgaag aattgcgatt
    13381 attattgaac ttccaactta atgaccgctt tctgctgacc ctttttctgg ttggtcagcc
    13441 ggaattaaat aacatgataa agaatatacc ccaacttaac cagcgaatag cggttcgttt
    13501 tcatttaaaa cactttaact ttgaggaaac caaagcctac attcaatatc gtcttaaagt
    13561 tgctggccgg aatgatcaga tatttaccga ttcagcattt gatgaaattt ataagtactc
    13621 agagggcatt cctcgaatta tcaataacat atgtgactta agcttggtga tcggttttgg
    13681 aaaacacctc tcaactattg accataatgt catcaaagga attattaatt cagaaagata
    13741 atatctatgg caggaatgca agacatcctg aaaaatagtc aggaatcaaa taaccaaaat
    13801 aattcccaaa agggaaaaaa cgaaaaaaat gataataatt tcgttggtct gagcgaaatg
    13861 gaagccgtaa aaaaagccat tactgaaaac cttggctcga caacgaatac atttaattca
    13921 gcggccgatt caacgccggt tgatttcgag ccggatttgg tgctaactcc ggctgatttc
    13981 cctaaaaatg gtttcttcaa agacagtcac ctcgatgaaa agaaactgaa agaaatccaa
    14041 tgccaggatg aaattgcagc caatcagctt tacatggatt tgcttcatga attgggaaat
    14101 gttttccaat ctctaaagga taataaacct ctcgaccccc aaccgttaga aaaatttatc
    14161 gatttgatgc ataattccat cacatccggc gatttcttgc tcctcaaagc aattcaacac
    14221 aaacgctatg ccgcctggct tatttcccat tcagccaatg tcacaattct ggcgtataaa
    14281 attggactag atttaaaata tgaccgccca acattacata agctcggaat ttcagcgcta
    14341 ttacacgata taggaatgct caaggtacct aataacatta tcttcaaaca tggtcagcta
    14401 acgccagatg aatttgagat catcaagaaa catccgctgg acggattcga aatggctaag
    14461 catcttgaac aatatccgca tattatcgag gtcattttac aggaacacga acgggaagat
    14521 ggctccggtt atccgcaagg cttaccaggc gacgaaatag cagaatttgc taaaatcgtg
    14581 ggtttggcgg atgttttcga agcgatggtt catggacgcg cctaccgcga aggtttcatc
    14641 acttacactg ctattcagaa aataatcgaa aagcgttcta aacaattcaa tcccagaatt
    14701 attcgcgccc ttatcaatag tgtaccaatg tttcccgttg gtagtttggt caaacttagt
    14761 accaacgaaa ttgccagagt gctttcggtc aacagcttgc ggccggttcg tccggtagtg
    14821 gaaattctaa ttgatagcga cggcaataga ttaaaaaatc cgattcggct cgatctcgag
    14881 gcagagccat taatttatat agctaagccg ctcgccgaat cctgagcgat tcccctcatt
    14941 ttcaaagtgc ctgactcagc cacaatctat gcggttggac aggcattttt
//
LOCUS       DAOOMU010000054        14990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4241_length_14990_cov_15.264948, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000054 DAOOMU010000000
VERSION     DAOOMU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 14990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14990
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4241_length_14990_cov_15.264948"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="PLI55_06420"
     CDS             complement(<1..1292)
                     /locus_tag="PLI55_06420"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB00505.1"
                     /translation="MIKAVNIKSSAENRRVLIADKNPDLCHTLSTFLVKNGCNCQSTN
                     DSATTLCLLEESEFDFLIIDLDLARYDENSILDKAHIISPRTFIILTTTNASVETAVG
                     AFRKGAIDYLIKPLNQRVLLDQINQAIQLREILNENRFLRREIHNQKNPDQIIGESPS
                     IKNIYHLISKVAHSSSNILITGRSGTGKELVARAIHQNSPRANAPFIAINCSAIPETL
                     FESELFGLKKGSFTGAIMDKDGVFRAANGGTLFLDEVGEIPVHTQVKLLRAIESKEIK
                     PIGSTNSLIVDVRILSATQKDLLKEIEEGNFREDLYYRLNIVEIHLPSLAERKEDIPL
                     LVDHFICTYNNELKRKVLGADKDTMKILMDYKWKGEVRELENIIERAVLLCEGDYITP
                     RDLPPGLREGKSTFSDIPDDLKSSVRNFERQHILGILRR"
     gene            1629..2411
                     /locus_tag="PLI55_06425"
     CDS             1629..2411
                     /locus_tag="PLI55_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020401732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPB00506.1"
                     /translation="MSKEDQEIIKLREEVMSQEVLPRHIAIIMDGNGRWAKQRGLPRV
                     AGHNEGINSVREVTRECGSLGIKVLTLYTFSVENWSRPKSEVSALMTLLLHTIRNEID
                     ELHKNNVRLTLIGHLTDLPDDPLKGLMEGVEKTSQNTGLNLNLALSYGGRVEILDATR
                     AIAKDVLEGRISLDDIDEKLFSDYLYTRDMPDPDLLIRTSGELRLSNFLLWQMAYTEI
                     YVTETLWPDFRNKELLLAIREYQKRERRFGKTSEQITSPSES"
     gene            2414..4783
                     /gene="bamA"
                     /locus_tag="PLI55_06430"
     CDS             2414..4783
                     /gene="bamA"
                     /locus_tag="PLI55_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPB00507.1"
                     /translation="MIKYTPISLFLLILMPFSLIMAQEEAGIMILGLAVQGHKTISES
                     SIKIQSGLVEGRRVTQEDIAQAIQRLWKLKMFSDIQILLDKETSEGVFLVIKVEEYPR
                     LEKFEVKGNKKIKKSKIEEELNLVQGRVLSANLIAEVKRKVKALYTKEGYLLAQVDAE
                     ITDGTKENSKNLILKIQEGKKVRIDEINFVGNEHFSDSRLRRVMKDTHERNLFILRTG
                     EFDETKYIEDKEKLRTFYRKEGFRDFKIISDSISYTPNKERMLITINLYEGPRYKYRN
                     INFSGNTVFTTEQLQMLLGIKPGDYYNEELLQKALYDRLNGAYMDRGYLFCQIMPLEV
                     PVAENEIDLTIEITENSIVSIDQININGNTKTNENVIRRELRMFPGDIFSRDALMRSQ
                     NEIYMLNYFANVTPDVVPVSENAVDVELTVEEKSTDQANLSFSISQVYGLVGGGGFQF
                     NNFRGRGQQLAISYQQGTSYSIYSTSVTPYKALSLSFADPWIFDSPNLVGASVSYSER
                     GSGSSYYYPYNLTIYGGSLRWGRRLRWPDNYFRISWSYGAYKKSYTDIGSAYLEEVLE
                     GKDRTTSIGLTQVISRDSRDRPEFPTTGSVFSWVTNLTGGLLGGTEHFTKNTFTLDYY
                     LPTIWKLILYNHVEFGVINKLKKGTAVPPDERFIMGGSGMIYGTALRGYDDNEVGPTY
                     YYGGYYYPYGGETMLKYSIEYRVPIANNPTIYALAFAEAGNVWKYMRDTDPFDLKRSV
                     GVGIRFYMPALGLLGIDFGYGFDDIDPEGTTGYGKPEGWKTHFIFGTAY"
     gene            4817..5338
                     /locus_tag="PLI55_06435"
     CDS             4817..5338
                     /locus_tag="PLI55_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPB00508.1"
                     /translation="MKKVVFIILGLLMIGASDLAQAQIKVGYVNSDRILSEFDEAREA
                     QTKLDIEAKKLEDQYRGMLTKLDSLQKDYDRQKMMMSETRRKTKETEISQLQESIQRF
                     QVEKLGPDGEIYKKQAELVTPILEKIKTVIAKVGKDGKYDFIFDTVAGNILYAEPAHD
                     VTDKVIYELKRTQ"
     gene            5341..6369
                     /gene="lpxD"
                     /locus_tag="PLI55_06440"
     CDS             5341..6369
                     /gene="lpxD"
                     /locus_tag="PLI55_06440"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPB00509.1"
                     /translation="MQLTLGQIARLVNGAVKGDENILINDVAEIQNAQSGAITFLANP
                     KYAKYLASTAATAVIVDENFNGNFPNLILVKNPNLAFSIVLAHFRPELPLPAPMIHPT
                     AVIAKDARIGDDVYIGPFVVIESGAQISDKVTVFGHCYVGQYVNIGSGTVIYPNVSIY
                     HKCIIGQKVRIHAGVVIGSDGFGFVRVENGIEKIPQAGRVVIHDEVEIGANTTIDRGT
                     IGDTVIGRGTKLDNLVQIAHNVRIGEYCFFAAQVGIAGSTIIEDRVNVGGQVGIAGHL
                     HIGTGVMIAAQSGVTKDVPAGTFLFGSPAQERLKAAQELAHIRSLPEIKERLKKVEME
                     LRKGKNIG"
     gene            6373..7770
                     /locus_tag="PLI55_06445"
     CDS             6373..7770
                     /locus_tag="PLI55_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HPB00510.1"
                     /translation="MSRHQRTIKNEVSISGIGLHTGVKSTITFKPAPENSWIKFVRTD
                     VENCPEIVADINHVTDISRGTTLSQNGVQVHTVEHVLSAVAGLGIDNIIIAINNIEPP
                     VCDGSAIEFVRALKRAEIVEQEAEQDILVIDKILTYSDPKKHVDIHITPAEKFQITFL
                     IDYSEIPAIGTQYSTTYSLEDEFETDYAAARTFCTLSEIEELKNRGLIKGGNVNNAVV
                     FVDRHIEASEVERLRELFGISSDISIGQGGTLNGVELRYYNEAVRHKIVDLIGDFALL
                     GVPIQGHVIAARSGHAHNINFVRKIRKEYEKVMISKKYQKGKVKGALFDISGIERILP
                     HRFPFLMVDKIVDMVPGESVTAIKNVTINEPFFQGHFPVKRVMPGVLVLECLAQTGGF
                     MLLNTIENPEKVLLYFTGLDEVRFRKPVVPGDQIRLEVKMVQSRLSMCKMRGVAYVND
                     EVVVEANMMASLVEL"
     gene            7774..8547
                     /gene="lpxA"
                     /locus_tag="PLI55_06450"
     CDS             7774..8547
                     /gene="lpxA"
                     /locus_tag="PLI55_06450"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPB00511.1"
                     /translation="MNKIHPTAIIDSQARLGENISIGPYSIIGNNVEISDGCQIGSHV
                     LILPGARIGRDCKIYHHAIISEIPQDLKFAGEETTAEIGERTIIREFVTINRGTSQHW
                     KTVIGSDCLLMAYVHIAHDCIIGNHVILANAVNLSGHVEVEDWAIVSGMVGVQQFSRI
                     GQHSIIGGHYRVPKDVPPYIMAAREPLRYEGLNLVGLKRRNFPPETILALKRAYTLIY
                     NSEYNVSDAIKKIEETPELMIPEVRNVIEFIKKSTRGII"
     gene            8550..9281
                     /locus_tag="PLI55_06455"
     CDS             8550..9281
                     /locus_tag="PLI55_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00512.1"
                     /translation="MNIRQFQKFKYLRFVGLFLIAANLSANELDLTALPKNQVRGELQ
                     NILPKLCAGLELPCILLSRPFTPGVLISARYNCPDLLDSPSNLTGLPTCGMAVGATPD
                     IILAGQIGTGRWQEETLNSMGIYLAYVWGKPTAPNQIIGGVNHTKGPVDFHFLDMSLG
                     YLKLFQFNQWLFSVAGTAHFTQVGIHVTDNLNSADNYKARKNIDFMLVNITLQRNLGE
                     HFQIGTNLTFSPKSVSGGIALGSYF"
     gene            9283..10449
                     /locus_tag="PLI55_06460"
     CDS             9283..10449
                     /locus_tag="PLI55_06460"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPB00513.1"
                     /translation="MAEAGKIRAISVLGSTGSIGVNALKVISASRDRFTVNYLTAGRN
                     GTLLIEQAKKFHPKAVAIADESQYRLLKENLSGTNIEILVGKDGILEITGRRDVDLVV
                     NAIVGMAGLEPTYRALTAGRNVALSNKESLVMAGEIIMNLIAQSGLNLFPIDSEHSAI
                     WQCLAGEDKVSVKRLILTGSGGPFRARPQAEFKSITPAEALRHPTWTMGRKITIDSAT
                     MMNKGLEIIEAFWLFGVEPEQIEVLIHPQSVIHSMVEFCDGSIKAQLGLPDMRLPIQY
                     AIGYPERLPIIWEEIEWAKLGPLTFEAPDPVKFPALRLAFEALKRGGTAPAIMNVINE
                     LAVYAFLDEKIGFMEITDIIEKALNEIPVIDKPNIEDILNVEPMAREFFGNFIR"
     gene            10468..11541
                     /gene="rseP"
                     /locus_tag="PLI55_06465"
     CDS             10468..11541
                     /gene="rseP"
                     /locus_tag="PLI55_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPB00514.1"
                     /translation="MLYTILAVLIVIGGVVIAHELGHFVFAKLTGMRVEVFSIGFPPK
                     IISKKIGETEYQLSAIPLGGYVKVSGVVDESFDTTAAESNEPWTFAGKKTWQKLLFIS
                     GGVIFNVLFAFVIFSGLTMHEGIYEPVDKPIVESVAPGLPADSLGIKPGDIILAINGT
                     AVESWSSMTELIHSHPYDSILIKWQSGGTIYEKKVKPAANKILKGSELVEVGLIGISP
                     TMNHRPAKFFESLDNGMQNTWYWLKITVISLKMLITGEESIKNIGGPIFIAQLAGETA
                     KFGFGSLLNLMAIISLNLALINILPVPAFDGGHLLITLIEAIKRRPLSVNAKVRIQQI
                     GIAIILILTVLVLFNDISRILKW"
     gene            11812..12015
                     /locus_tag="PLI55_06470"
     CDS             11812..12015
                     /locus_tag="PLI55_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPB00515.1"
                     /translation="MKKIYRSRTDQKLAGICGGLGEIFNIDSNLIRLALVFATILTGI
                     FPLLITYIVGWIIIPEGPVEGKG"
     gene            12083..12760
                     /locus_tag="PLI55_06475"
     CDS             12083..12760
                     /locus_tag="PLI55_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HPB00516.1"
                     /translation="MLGTLVNVGTVILGSSLGLLLHTRLPRKFTVIVFQVIGLFTLFL
                     GFSMALKTTNFLIMVFSSALGAVIGELCKIEEAINNLGENLKRRLKSDNDKFAEGLVT
                     AFLMFCMGSMTILGAFEEGMGGEPNLLLAKSLLDGFSSMILSAGLGVGVLLSVIPLFL
                     YQGGLTLLAGLLGDVLNPTVINEMTAVGGLLLIGLGLTILEIKTIKVVNMLPGLVVAI
                     ILAGIFL"
     gene            12914..14977
                     /locus_tag="PLI55_06480"
     CDS             12914..14977
                     /locus_tag="PLI55_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPB00517.1"
                     /translation="MLSGGGARCFAQIGVIKALEEIGFYPDFIVGTSFGALIGALYAG
                     GSTPQEIEEFIANTRWNRVLASQPFRDIEFVSQKIRTLPELFTLRFDENFNVIFPRNL
                     LSTQALQERIFQMTIYPEYYAGSDFDSLAIPLRIIATDLKTGKMVVLSNGNLARSVTA
                     SSAFPIILAPVKYGTYLLADGGLTDNVPIDVALDLGAEFIVAVDVSSKIVPLAENFDV
                     FDIFGQAMNTLAYPSDTKNLELADVLIRPEIDHITSTDFDSLNSLVESGYRSCQKYWG
                     KIQPYADNICKSPDFLENAINKLNNTSINQIKYSSDQATREYILRREMELKEGDRWNL
                     KLAQRSMKNLYSTGLFKNVYLSLSEVDSSRADLLVEVEEHERTAFSFGARYDSERKAS
                     AFISGKYRNLLGRGIDNQVNLIVSDLLFRAEWNSRTIRVLSSNFTGYTSLYHLNESIP
                     LYNNDGRRVESGKLYRTGLEANAGIQIKRVGLTAFGLKWENTVADSTIHYIPRVNHDN
                     YSVGSLTLRILVDNVDDPDLPKSGRVNDIRYEHSIERDRLKQFDRISVESVTYETFDE
                     KYTFSTTLHLGYLNKVLNHYEQFRLGGLNSLPGYHQDELWGSMIGVLGLGCRAPLTSG
                     IYYRLSGMVGNVWDGFSDFDWRQSRAGISLGIVIPTPIGPISADYGIGSHNHRLFYIS
                     VGRYF"
BASE COUNT         4175 a         2777 c         3578 g         4460 t
ORIGIN      
        1 cgccgcagaa ttcctagaat atgctggcgt tcaaaattgc ggacggaaga tttgaggtcg
       61 tccggaatat ctgagaaggt tgattttcct tccctaagac cgggcggcaa atcgcgcgga
      121 gtgatataat cgccttcaca gaggaggacg gcacgttcga tgatattttc gagttcgcgt
      181 acttcgccct tccatttata atccattaga attttcatcg tatctttgtc ggcgcccaat
      241 actttgcgtt tgagttcatt attataagta cagataaaat ggtcaaccag aaggggaata
      301 tcttctttgc gttcggctag cgagggcagg tgaatttcga cgatattcaa gcggtaatag
      361 aggtcttcgc ggaagttgcc ctcttcgatt tccttgagta aatctttttg agttgccgaa
      421 agaatccgaa cgtccacaat cagagaattt gtactgccga ttggtttgat ctctttgctt
      481 tcgatggcgc ggagtaattt aacctgggta tgaaccggaa tttcgccgac ttcatcgaga
      541 aatagcgttc cgccgtttgc ggctcggaaa acaccatctt tatccataat tgcaccggtg
      601 aaggagcctt ttttcaaacc aaatagttcg ctttcgaaga gtgtctcggg gattgcgctg
      661 caattaatgg cgataaaggg tgcgtttgcg cggggactgt tttggtgaat agcccgcgct
      721 actagttcct ttccggtacc gctccggcca gtaatcagaa tgttagatga agaatgggcg
      781 accttggaaa ttagatgata aatgttctta attgagggac tttcgccgat aatttggtcg
      841 ggattttttt gattgtgaat ttctctgcga aggaaccggt tttcatttag aatttctcta
      901 agttgaatag cttggttaat ttggtcgagc aggacgcgct gattgagtgg ttttatcaaa
      961 taatcaatag cgcctttacg gaaagcgcca acggcagttt caacggaagc attcgtagtg
     1021 gttaaaatta tgaaagtgcg gggactgatg atatgagctt tgtccagaat tgagttctca
     1081 tcgtatctcg ctaaatccag atcaataatc aaaaagtcga actccgattc ttccagcaga
     1141 cacagagtgg tagcgctgtc gtttgtcgac tggcagttgc acccgttttt aaccagaaag
     1201 gtacttaaag tgtgacataa gtccggattt ttatcggcta tcagtactcg tcggttttct
     1261 gcacttgact ttatattgac cgccttgatc atttaaattt cttagattca atatagaaca
     1321 gagtaagttt taatcaaaaa cattttttaa atctcggaaa tgagaagtaa ttaacggctc
     1381 gttttaagta aatgaatatt ttacgaggaa caatcctaat tgcctgccgt aaaatgagcc
     1441 gaagttatga ctattaagaa aacacaaacc taaaacattt ctcttaatcg agaatctgga
     1501 aattgaacag ttcatttaga ccagccgata tattctttat tacctaattc accaaaattt
     1561 taaatggttt tcattgactt tataggcttt atataataat ttctgtagtt tatttaactt
     1621 tgaatcaaat gtccaaagaa gatcaggaaa taatcaaatt acgagaagag gtaatgagcc
     1681 aggaggtctt accgcggcac attgctatca ttatggatgg gaacggtcgc tgggcaaaac
     1741 agcggggact accgcgggta gccggtcaca acgaaggaat caattcagtt cgggaagtta
     1801 cgcgcgaatg cggtagtctt ggcatcaaag tgctgactct atataccttc tcggtggaga
     1861 actggtcacg ccccaaatcg gaagtttctg cccttatgac cttgctgttg cacacaatcc
     1921 gtaatgaaat agatgagttg cataaaaata acgtgcgtct gactttaatt gggcacctta
     1981 cggatttgcc ggacgatccg ttgaaagggt tgatggaagg agtcgaaaag accagtcaaa
     2041 ataccggact gaacctgaat ctggcattga gttacggcgg gcgggttgaa atattagatg
     2101 ccactcgtgc cattgctaaa gatgtgctgg aagggcgaat cagccttgat gatattgatg
     2161 agaagctatt ttccgattat ttatatacac gcgatatgcc tgatcctgac ttattgatca
     2221 gaacgagtgg ggaactgcgc ctcagtaatt ttttactctg gcaaatggct tataccgaga
     2281 tatatgttac ggagacttta tggcctgatt tccgtaataa agaactactt ttggcgatcc
     2341 gtgagtatca gaagcgagaa cgtcgttttg gaaaaaccag tgaacaaatt accagtcctt
     2401 cagaaagttg agcatgataa aatatacgcc gattagtttg tttctattga tattaatgcc
     2461 gttctcgctt ataatggctc aggaagaagc cgggataatg atcctcgggc tggcggtgca
     2521 gggtcataag accatttcgg aatccagcat caaaattcag tccggattag ttgaaggccg
     2581 aagagttact caagaagata ttgctcaggc tattcaacga ctttggaagc tcaaaatgtt
     2641 ttccgatatt caaattttgc tcgataagga aacgagtgaa ggagtgtttc tggtaataaa
     2701 agtggaagaa tatccgcgtt tggagaagtt cgaggtcaag ggcaataaga aaatcaagaa
     2761 gagtaagata gaagaagaac ttaacctagt tcagggaagg gtcttatctg ctaacttgat
     2821 cgcagaagtc aagcgcaaag tcaaggctct ttatacgaag gaaggttatc tcctggctca
     2881 agttgatgcg gaaatcaccg atggcactaa ggagaacagc aaaaacttga ttctcaagat
     2941 tcaggaaggg aaaaaagttc ggattgatga aatcaacttt gtcggcaatg agcatttttc
     3001 ggatagtcgt ttacgtcggg taatgaaaga tactcatgaa cgcaatctat ttattttgcg
     3061 cactggggaa ttcgatgaga ctaaatatat cgaggataaa gaaaaattgc gcacctttta
     3121 tcgcaaagaa ggtttccggg attttaaaat catttcagat tcgatttctt atactccgaa
     3181 taaggagcgt atgttgataa caatcaatct ttatgaagga ccacgttata aatatcgcaa
     3241 tataaatttt agtggaaata cggtattcac cactgagcag ctccaaatgt tactcggcat
     3301 taaaccgggt gattattata atgaggaatt gttgcagaaa gcactttatg atcgcctcaa
     3361 tggagcatat atggatcgcg gttatttatt ttgtcaaata atgccgctgg aggttccggt
     3421 tgcggagaat gaaattgacc ttaccattga gataaccgaa aattcgatcg tatcaattga
     3481 ccaaattaac attaatggca atactaaaac caacgaaaat gttattcggc gcgaattgag
     3541 aatgtttccc ggcgatattt ttagtcgcga cgccttaatg cgcagtcaaa atgaaattta
     3601 tatgcttaat tatttcgcga atgtgactcc ggatgtagta ccggttagtg aaaacgcagt
     3661 agatgttgag ttgactgtgg aggagaaatc caccgatcag gcaaatttat ctttcagtat
     3721 aagccaggtt tatggtctgg tgggtggagg cggatttcaa ttcaacaatt ttcgaggacg
     3781 cggtcaacag ttggcaatca gttatcagca aggaacaagc tactccatct atagtacgtc
     3841 ggttacgccg tataaagctc tctcccttag tttcgctgac ccctggattt tcgattcacc
     3901 caatttagtc ggtgctagtg tttcctattc ggaaagagga agcggcagta gttactatta
     3961 tccgtataat ttgacgattt acggaggatc tctgcgctgg gggagacgtt tacgctggcc
     4021 agataattat tttcggataa gctggtcgta cggggcttat aaaaagagtt ataccgacat
     4081 cggaagtgcg tacttagaag aagtgctgga aggtaaagat cgaacgacga gtatcggact
     4141 tactcaggta ataagtcgtg acagtcgtga tcgcccggaa tttccaacga ctggttctgt
     4201 tttcagctgg gtaacaaact tgaccggtgg tttgttaggt gggactgagc atttcaccaa
     4261 aaatactttt accttggatt actacttacc gacaatttgg aaactgatac tgtataatca
     4321 tgttgaattt ggagttatca ataaattaaa gaaaggtacc gctgtgccac ccgacgagcg
     4381 atttataatg ggcggcagtg gtatgattta cggtacagcc ctacgcggtt atgacgataa
     4441 tgaagtcggc ccgacttatt attacggtgg ttattattat ccgtatggtg gcgaaacaat
     4501 gctgaaatac tctattgaat atcgggtgcc gatcgcgaac aatccgacta tttatgcctt
     4561 ggcatttgcg gaagctggca atgtctggaa atatatgcga gataccgatc cttttgacct
     4621 taaacggtcg gtaggtgttg ggatacgctt ttatatgcca gcgctcggat tattaggtat
     4681 tgattttggt tatggatttg acgatattga ccctgaaggt acgaccggtt atggtaaacc
     4741 tgaagggtgg aaaacccatt ttatttttgg tacagcttat taacaataaa aacaaatgtg
     4801 taacaatgga ggaaaagtga aaaaagtagt gttcattatt cttggattgt tgatgatcgg
     4861 agccagtgac ttagcgcagg ctcaaattaa agtcggctac gtcaattccg atcggattct
     4921 gagtgaattt gacgaagcgc gggaagcgca gactaaactg gacattgaag ccaagaaact
     4981 ggaggaccag tatcgcggta tgcttactaa attggacagt ctgcagaaag actacgatcg
     5041 tcagaaaatg atgatgagtg aaacgcgacg caagactaag gaaaccgaaa ttagtcaatt
     5101 acaggaatcg attcaaagat tccaggtcga aaagcttgga ccggatggcg agatttacaa
     5161 gaaacaagcc gaattggtaa ctccgattct ggaaaaaatc aaaaccgtca tagccaaggt
     5221 ggggaaagac gggaagtacg attttatttt tgatacggtt gctggcaata ttttatatgc
     5281 cgaaccggct cacgacgtta ctgacaaagt catctatgag ttaaaacgca ctcaataggt
     5341 atgcaattaa ccttagggca gattgcccgg ttagtaaacg gcgcagttaa aggcgacgaa
     5401 aatattctta tcaatgacgt tgccgaaatt cagaacgctc agagcggtgc tattactttt
     5461 ttagctaatc cgaaatatgc gaaatattta gcaagcaccg ccgccacggc tgtgatagta
     5521 gatgagaatt tcaatggcaa ttttcctaat ttgatattgg taaagaatcc taacctcgct
     5581 ttttcgattg ttctggctca ttttcgaccg gagttgcctt tgccagctcc tatgattcac
     5641 cccacagccg tgatcgcaaa agatgccaga attggagatg atgtatatat tgggccattc
     5701 gttgtcatag aatccggtgc gcaaattagt gacaaggtca cggtctttgg acattgttat
     5761 gttggtcaat atgtaaatat cggtagcgga acggttatct atccgaatgt cagtatctat
     5821 cataaatgta taattggtca aaaggtgcgc attcatgctg gagttgtgat cggaagtgat
     5881 ggatttggtt ttgtacgcgt tgaaaatggc attgagaaaa ttccgcaagc cggtagagtt
     5941 gtaatccacg atgaggtaga gatcggcgct aatacaacca tagatcgggg gacaatcggt
     6001 gatacagtca ttggccgcgg taccaaatta gacaatttgg tgcagattgc ccataacgtt
     6061 agaattggtg aatattgttt cttcgcggct caggtcggaa ttgccggtag tacgattatt
     6121 gaagatcgag taaacgttgg cggtcaggtc ggcattgccg gtcatttgca tattggtacg
     6181 ggagttatga ttgcggctca atcgggtgtt accaaagatg tcccagccgg gacatttcta
     6241 ttcggttctc cagctcagga acgtttgaaa gccgcccagg agttagccca tattcgttct
     6301 ttacccgaaa taaaagagcg tctcaagaaa gtagaaatgg aattacgaaa aggaaaaaat
     6361 ataggataag ttatgtcacg ccatcagcga actattaaaa atgaagtctc gatttccggt
     6421 attggactcc atactggcgt aaagagtact attactttta aaccggcgcc tgagaattcc
     6481 tggattaaat ttgtacgaac tgatgtcgaa aattgtcccg aaattgtggc agatattaat
     6541 catgtaaccg atatttcgcg cggcacgact ctctcgcaga acggggttca agttcacacc
     6601 gttgagcacg ttttatcggc tgtagctggt ttgggaattg ataatatcat aattgcaata
     6661 aataatatcg agccaccggt ctgtgatggg agcgctatag aatttgtgcg agctttgaaa
     6721 cgggctgaaa ttgtagaaca agaagcggag caagatattc tggtcataga taagattctg
     6781 acctactctg atccgaaaaa acatgttgat attcacatca cacccgctga aaaatttcag
     6841 attacctttt taattgacta cagtgaaatt ccggctatcg gcacgcagta ttcgaccact
     6901 tattcgctcg aagacgaatt tgagactgat tatgccgccg ctcgcacttt ttgtactttg
     6961 agtgaaattg aagaacttaa aaatcgcggt ttaattaaag gcggtaatgt caataacgcc
     7021 gtggtttttg tagaccggca tattgaggcg agtgaagttg aacggcttcg tgaattattt
     7081 gggatttcca gtgatatttc cattggtcaa ggcggtacgc taaacggggt agaattgcgt
     7141 tattataacg aagccgtgcg gcataaaatc gttgatttaa ttggtgattt tgccctgctg
     7201 ggtgttccga ttcagggaca cgttatcgcc gcgcgaagtg gtcacgccca taacattaac
     7261 tttgtccgga aaatacgcaa ggaatacgaa aaggttatga ttagtaaaaa ataccagaaa
     7321 ggcaaagtta aaggggcttt atttgatatt agcggcatcg agcgtattct tccgcatcgt
     7381 tttccattct taatggtaga taaaattgtt gatatggttc ccggtgaatc ggtcactgca
     7441 attaaaaacg ttacaattaa tgagccgttt ttccaagggc attttccggt taagcgggtc
     7501 atgccggggg tgctggttct ggaatgcctg gcgcagactg gcggatttat gcttttgaat
     7561 acgattgaaa atcccgaaaa ggtcttatta tattttaccg gattggatga ggtcagattc
     7621 cgaaagccgg tagtgccggg cgaccagatt cgccttgagg ttaaaatggt tcaatcccgt
     7681 ttatcaatgt gtaaaatgcg aggcgtcgct tatgtcaatg atgaagtcgt cgtcgaggca
     7741 aatatgatgg cttcgctagt ggaattatag aatatgaata aaattcatcc tactgctatt
     7801 attgattccc aagctcgact tggtgaaaat atttcaattg gtccatacag tatcatcggc
     7861 aacaatgttg aaattagtga cggctgtcag attggaagtc atgtattaat tttacccggc
     7921 gctcgaatcg gtcgtgactg taaaatctat catcatgcca taatttccga aattcctcag
     7981 gatcttaaat ttgctggcga ggaaaccact gccgaaatcg gtgagcgaac aatcattcgc
     8041 gaatttgtca ctattaatcg tgggacatct caacattgga aaacagttat cggctcggat
     8101 tgtcttttga tggcgtatgt ccatatcgcc cacgattgta ttattggtaa tcacgttatt
     8161 ttggctaatg ctgtcaattt atccggacat gtcgaggtag aggattgggc aatcgtcagc
     8221 ggtatggtgg gtgttcagca atttagccgg atcggtcagc attcaattat cggtggacat
     8281 taccgcgttc ctaaggatgt cccgccctat attatggcgg cgcgtgagcc actccgttat
     8341 gaaggtttga atctagttgg attaaagcga cggaattttc ctcctgaaac tattttggct
     8401 ttgaaacgag cctatacctt gatctataat agtgaataca atgtctccga tgctattaaa
     8461 aaaattgaag aaacaccgga actgatgatt ccggaagtac gcaatgtcat tgaattcatt
     8521 aaaaaaagca cacgtggcat tatctgatat tgaatattcg gcaatttcaa aaattcaaat
     8581 atttacgatt cgttggatta tttctaattg ccgccaattt atcagccaat gagctagatc
     8641 tgacagcact gcccaaaaac caggtgcgcg gcgaattgca aaatattttg cccaaattgt
     8701 gtgccggatt agaattacct tgtattcttt taagtcgtcc tttcaccccg ggtgtattaa
     8761 ttagcgctcg ctataattgt ccggatttac tggattcacc ttcaaatctg acgggactgc
     8821 caacttgcgg gatggctgta ggtgccactc ccgatattat tttagccggg cagattggta
     8881 ctggccgctg gcaggaggaa acgctaaatt ctatgggaat ttacctcgct tatgtctggg
     8941 ggaagccgac tgcaccgaat cagattattg gcggagtcaa ccacaccaaa ggcccggttg
     9001 attttcattt tttagatatg tctctgggct atttaaaatt atttcagttt aatcaatggc
     9061 ttttttccgt tgccggcaca gcccatttta cccaagtcgg aattcacgtt actgacaact
     9121 tgaattcggc tgataattac aaagctagga aaaatataga ttttatgctg gtaaatatta
     9181 ctcttcagcg taacttgggt gagcattttc aaattggtac caatctaact ttttcgccga
     9241 aaagtgtttc aggcggtatc gcgctaggaa gttatttcta agatggcgga agcaggtaaa
     9301 attagggcaa tctcagttct gggttcgacc ggttcgatcg gcgtcaatgc tctaaaggtt
     9361 atttctgcga gccgggatag atttactgtt aattacctta ctgctggtcg aaatgggact
     9421 ttacttattg aacaggctaa gaaatttcac cctaaggcag tcgccatcgc agatgaatcg
     9481 caataccgat tattaaaaga aaacctctcc ggtacgaata ttgagatttt agtcgggaag
     9541 gacggcatac tggagataac cggtcgtcgg gatgttgatt tagtggtgaa tgctattgtc
     9601 gggatggcag gtttagaacc gacctatcgg gctttgactg ccggtcgtaa cgttgctctt
     9661 tctaataaag aatcgctggt tatggctggt gaaattatta tgaacctaat tgcgcaaagc
     9721 ggcttgaatc ttttcccgat tgatagcgaa catagtgcca tctggcaatg tttagccggt
     9781 gaggacaaag tcagtgttaa gcgtttgatt ttgacaggat ccggtggacc gttccgcgcc
     9841 cgtcctcagg cggaattcaa aagtattact ccggctgagg ctctacgcca tccgacctgg
     9901 actatgggga gaaaaattac tattgattca gcgacaatga tgaataaagg ccttgagatt
     9961 atcgaagctt tttggttatt cggcgtcgag ccggagcaga tcgaggtttt aatccatccg
    10021 caatcggtaa ttcattcgat ggtggaattt tgtgatggtt cgatcaaagc ccaactgggt
    10081 ttacccgata tgcgcttgcc gattcaatat gctatcggtt accctgagcg tctgccgatt
    10141 atctgggaag aaattgaatg ggctaaactc ggtccgctga ctttcgaggc gccggacccc
    10201 gtgaaatttc cggccttacg gcttgccttt gaggctttaa agcgtggcgg cacggcgccg
    10261 gcgattatga atgtgattaa tgaactggcg gtttatgctt ttctggatga gaaaatcgga
    10321 tttatggaaa taactgatat aattgaaaaa gcgctgaatg agattccggt tattgataag
    10381 ccgaatatag aagatatttt aaatgttgaa ccaatggcac gggaattttt tggtaatttt
    10441 ataaggtaaa gaaaggaaga gttatagatg ctttatacta ttttagcggt tttgattgta
    10501 attggcggag tggttattgc gcacgaattg ggacattttg tttttgctaa attgaccggt
    10561 atgcgggtgg aggtcttttc aatcggtttt ccccccaaaa taattagtaa gaaaatcggt
    10621 gaaacggaat accagctctc ggcgattcct ctgggcggct atgtcaaagt ctccggtgta
    10681 gttgacgaga gttttgatac taccgccgcc gaaagcaacg agccttggac attcgccggt
    10741 aaaaagactt ggcaaaaact actttttatt tccggcggag taattttcaa tgtccttttt
    10801 gctttcgtga ttttcagcgg attgactatg cacgagggaa tttatgaacc ggtagataaa
    10861 ccgatcgttg aaagcgttgc tcctggttta ccggcggatt cgttggggat caaacccggt
    10921 gatataatcc ttgcgattaa tggcacagcg gtagaatctt ggagcagtat gaccgagttg
    10981 attcatagtc atccctatga ttctatttta attaaatggc agagtggcgg cacgatttac
    11041 gaaaagaagg taaagccagc cgctaataaa atattgaagg gttctgaatt agtcgaagtt
    11101 ggtctgattg gaatttcgcc gacgatgaat caccgcccgg caaaattttt cgaatcgctt
    11161 gataatggta tgcaaaatac ctggtactgg cttaagataa ctgtaatttc cttgaaaatg
    11221 ctcattactg gcgaagaatc tataaagaat atcggcggac cgattttcat tgcccaatta
    11281 gctggagaga ccgctaaatt cggatttggt tcccttctaa acctgatggc gattataagt
    11341 ctcaatttgg cgcttattaa tattttgccg gtgccggctt ttgacggcgg gcatcttctg
    11401 attactttaa ttgaagctat taaacgccgt ccactttcgg ttaacgccaa ggtgcgcatc
    11461 cagcaaattg gtatagcgat tatcttaatt ttaacggtgc tggttctttt taacgatatt
    11521 tcccgtattc ttaagtggta gaaacggaag ttcgtcgtta gtagcgggta attaagctca
    11581 gtccgcatcc tattttcaaa ggcacttgcc tgaatcctga aaccgacctg gcttttaggc
    11641 tgaataagct cccgcaggct gacggatttt gtcaatattc gtcgaaattc aaatgaatat
    11701 ttattgataa tgattagaaa acacccacaa ttacccaatt atttaaaaat cttgctttcc
    11761 aacttccgaa ataatcaata aaattggagt gattttaacg gagaagtgtt tatgaagaaa
    11821 atctatcgtt cgagaactga ccaaaagtta gccggaatct gcggcgggtt aggtgaaatt
    11881 tttaacattg actcgaacct aattcgtctg gcgttggtct ttgctacgat tctaaccgga
    11941 atttttccat tattgattac ctatattgtg gggtggatca ttattccgga gggaccggta
    12001 gaagggaaag gttaagactg agttaaggct aagattaggc gggagtgtcg aagattctcg
    12061 actattagga gcggatgtcg ttatgttggg cactttggta aatgtcggga cggtgatact
    12121 gggcagtagt ctgggtttgt tgttgcacac ccgattgccg cgcaaattta cggtaattgt
    12181 ttttcaggta atcgggttat ttaccttgtt tcttggtttc agtatggcgt tgaaaactac
    12241 taattttttg attatggttt tcagtagcgc gctgggagcg gtgatcggtg aactatgtaa
    12301 aatagaggag gcaatcaata atctcggtga aaatctcaag cgtcgtttaa aaagcgataa
    12361 cgataaattc gctgaaggtt tggttactgc ctttctaatg ttctgtatgg gttcaatgac
    12421 tattttaggc gcttttgaag aagggatggg tggcgaaccg aatttgttat tagcgaaatc
    12481 actgctggat ggattttctt caatgatttt gtctgccggt ctgggagttg gagttttgtt
    12541 atctgtgatc ccgttgttcc tttatcaagg tggtttgacg cttttagccg gtctcttggg
    12601 tgatgtttta aatccaaccg tgattaatga aatgacggct gtcgggggtt tattgctaat
    12661 cggtctcgga ttgacgattc tggaaattaa gaccatcaag gtggttaata tgttgcccgg
    12721 tctggttgta gcaataattc tggctggcat ttttctttag ttgattttct gattgtcagc
    12781 gccgttcgaa tgagttttat atcagtcaat gatttcaaat ttaaaaaaca ttaagttcag
    12841 taaaatctta cttataatct gtctgatatt taattcgagg cttttagctg atgataagcc
    12901 caaaactgcg ttggtgttaa gtgggggcgg ggcacgttgt tttgcccaaa ttggagttat
    12961 taaagccctg gaggaaatcg gcttttatcc cgattttatc gttgggacaa gttttggggc
    13021 tcttattggg gctttgtatg ctggtgggag tacgcctcaa gaaatcgagg aatttattgc
    13081 taatacgcgc tggaaccgag ttttggcttc tcagccattt cgcgatattg agtttgtatc
    13141 ccagaagatt aggactttac cagagttgtt tactttgcgg tttgacgaga acttcaatgt
    13201 gatatttccc cgtaatttac tttcaacgca agctttacaa gaacgcatct ttcaaatgac
    13261 gatctatccg gagtattatg ctggatcgga tttcgatagt ctggcaatcc cgttacgaat
    13321 tatcgctact gatctaaaaa cgggcaaaat ggttgttctg agtaacggca atcttgcccg
    13381 gtcggttacc gccagttcgg cttttccaat cattctagcg ccggtcaagt atggaacata
    13441 tctgttagcc gatggtggct tgaccgacaa tgtcccgatt gatgtggcac tcgatctggg
    13501 tgctgaattc attgtagctg tggacgttag tagtaaaata gtgccactgg cggaaaattt
    13561 cgatgtattt gacatttttg ggcaggcgat gaatacttta gcctatcctt ctgatacgaa
    13621 aaatctcgaa ttggctgatg ttctcatccg accggagata gaccatatta cttcgaccga
    13681 tttcgattca ctgaattctc tagtcgagag tggctatcgc agttgccaga aatattgggg
    13741 aaaaatacaa ccttatgcag acaatatttg taaatcaccg gattttttag aaaatgccat
    13801 aaataaatta aataatactt caatcaatca aattaaatat tccagcgatc aggcaacccg
    13861 cgagtacatt ttacggcgtg aaatggagtt aaaagaaggt gaccggtgga atttaaaact
    13921 ggcacaacgc agtatgaaaa acctgtatag taccggatta tttaaaaatg tttacctatc
    13981 tttgtctgag gttgattcca gtcgagcgga tttattagtc gaagttgaag aacacgaacg
    14041 cacggcattc agtttcgggg cgcgttatga tagcgagcgt aaagcgagtg cctttatttc
    14101 cggaaagtat cgaaatttac tgggcagagg cattgacaat caggttaatt tgattgtcag
    14161 tgaccttttg tttcgggctg aatggaattc ccgaactatt cgtgtgctta gttcgaattt
    14221 taccggttat acttctttgt atcatttgaa tgaaagtatc ccgctttata ataatgatgg
    14281 gcgacgcgtc gaatccggta aattataccg aaccggactg gaggctaatg ccggaatcca
    14341 gatcaaacgg gtcggactaa ccgcctttgg tctgaaatgg gaaaatactg ttgccgattc
    14401 taccatccat tatattcccc gcgttaatca tgataactac tcggttggca gtttaactct
    14461 gcggatatta gttgataatg ttgatgatcc cgatttacct aaatccggtc gagtaaatga
    14521 tattcggtat gaacattcaa tcgaacgaga tcgcttaaag caattcgatc gaatatcggt
    14581 cgaatcagtg acctatgaaa ctttcgatga aaaatatact ttttcgacta ctcttcatct
    14641 tggttatttg aataaagtgc tgaatcatta cgagcaattc cgacttggtg gattaaattc
    14701 gttgcctggt tatcatcagg atgaattatg gggttcgatg ataggtgtgc tcgggctggg
    14761 atgtagggcg cctttgactt ccggtatcta ttatcgtctg tccggaatgg ttggcaatgt
    14821 atgggatgga ttcagtgatt ttgactggag acaatctcga gcaggtatca gtcttgggat
    14881 tgtgataccg acaccgattg gaccgatttc ggctgattat ggcattggtt ctcataatca
    14941 ccggcttttc tatatttcag tcggacgtta cttttaatga tctcaaagca
//
LOCUS       DAOOMU010000055        14413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4518_length_14413_cov_18.292589, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000055 DAOOMU010000000
VERSION     DAOOMU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 14413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14413
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4518_length_14413_cov_18.292589"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLI55_06485"
     CDS             complement(<1..935)
                     /locus_tag="PLI55_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00518.1"
                     /translation="MRMRTVVISVVLIMALSSIAMPQSLVRESFSYPATTLAGLGTAS
                     NGFSGSWVTDEAGGIEGLVAVSGTRFAYADLNYEIPHDTLHLQVVKSNAWADHNRYKR
                     PLDGAWPNEAGKKYWVSYLLDIKEPLPIENTYFMVKLYKGSGELLAIGKAGGGPTPPV
                     FTCGSGWVGEGDDVSATPITVGPVWIVTMVKMSGGTGSDRTYMWIDPDPSVEPDTNDA
                     IVKRNSSMPEGFDHIALEFGGDGADVRLVFDEITVASSFADLTAETPTGTVARESFSY
                     PATSVAGLGSAGSGFSGPWVTDEAGGIEGLVAVSGT"
     gene            complement(1302..5540)
                     /locus_tag="PLI55_06490"
     CDS             complement(1302..5540)
                     /locus_tag="PLI55_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPB00519.1"
                     /translation="MGSFLRKISGLTIAPILLALSSNAFSAGLPTLRFEHLSTQDGLS
                     HNSIIEIIQDRQGFMWFGTLNGLDRYDGYNFTAYRPILGDTTSLCHNVINALYEDRRG
                     ILWIGTLDGLNRYDRARDCFIRIRHNPNNPNSLVDNRIRCIAEDNYGQIWIGTEGGLS
                     CYDPRVDTFQNYLPDPNNPNGLGDFIIHDIYNDSRDQLWIATEKEGVYRFNRITQSFT
                     RYRHETTESPKAGTSAVITIGEDRWGNLWIGTWGDGLQRFDVNAQRFRKVPIFCKGKK
                     PFTTKIITKLLIDQSDRMWMSTYDAGLFVIPQIPRNVTTAGIEGIQHYLCEPLDETSI
                     QSNALWTVYLDRTGVVWIGNENGGINKCDTKQSHILHYRFGLSGANSLSNNHVTSFFE
                     DQYGKIWIGTRFGGLNRFDPVANHFRVYRKDEQNNSLGSDAILCLTGNRDYLWIGTDG
                     SGLDRYDFKSGQFKHFRHDEQNPSSLGENAVYALYLDKSNTLWVGTWGGGLSKLNSDG
                     QTFSQYPIDRINDRLNVVTSIAEDTSGGLWLGTYGKGLVFFNPNNEKMLFFRPDDTDS
                     LSLGHENINTLFVSQDGTLWIGTMGGGLNYVQYFQADSGIAHFGRYDQRHGLPDNIIE
                     SLIEDGDGFIWMGTNHGISRLNPANDEIHNFDRNDGFGQDNFYRESVLRASNGMLYFG
                     GINGFNVFQPGSISYNPFIPPVVITQFQIFNETISPNTQGKIKLDSSIFITENIILSY
                     RENVFSFEFAALDYSCPQKNQYAYIMEGFDQDWRRTDANRRFATYTNLPPGNYTFRVK
                     ASNNYGVWNESGTAIHLKITPPFWQTNWMLIIYIGLILGAILLIRMAVQVRERNRAQA
                     EMRRLEAEKTHELDQLKLRFFTHISHEFRTPLTLIIGPVERMLHLGNKLSQEKRDVYN
                     RLVLQNARRLLRLVNQLMDARKLDTGSMKLELQQKEFISFVRAIFSVFHLQAEQRNIR
                     YIFKTELEEQYFYFDPDKIEKALYNVIANALKFTNPGGMVEVSLFLAPPNMESYSKRE
                     EAERSICVQVRDTGIGIPPDQIEHIFDPFYQVRQPETRKMHGTGIGLSISRDFIEMHG
                     GRIIVESTPGKGSTFSLIIPMRTMAEALKTGEQEVGEQDYIGEMAELIPDENLKISNE
                     LTESKLSMKNSDKAKPLILIVEDDNDLRHYLAMELESYYSVIQAADGLTGYELAVDRI
                     PDLIICDVLMQVMDGNEFCHRCKNDQRTSHIPIILLTAQTSEEKRKEGLLAGADDYIT
                     KPFNPDLLRIRIENLLNTRRKIRERFGHMVYMNPKEINVSSPDEQFLQRTVEVVEQYL
                     SDSGFSVDDLSYHVGLSRAQLYRKMQGLINQTPSEFIRNYRLQRAMQLLEKGHSPSQV
                     CYQIGFKDPSYFTKCFRKQFGRTPQQIRPESELS"
     gene            complement(5604..7364)
                     /locus_tag="PLI55_06495"
     CDS             complement(5604..7364)
                     /locus_tag="PLI55_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPB00520.1"
                     /translation="MHRRMVTLILTLMLIVGCSEDKSAVDISIDSAEITGQTIVPPGP
                     EWLHNAVFYQIYPQTFYDSNGDGIGDLNGIIEKLDYVQSLGVDGFWVNPFFASPFNDA
                     GYDISDYYQVAPRYGTNQDARRLFLEAEKRGLKVLFDYVISYTSIEHPWFQESARQEP
                     NKYSNWYVWTNNTWIDPPEAYRDAFIKGYSRRNGQFMRNFYWSQPALNFGFVKPESDW
                     MLPIDHPDVLALQEELQKVLRFWMDMGADGFRADMAGALVKNANIVEGDPEYANRDEG
                     TKIFWRKIRKIIDEEYPQAFMVAEWSYPVAALDGGGFHADFFHWFSGYNDLLQKESWR
                     IMNGHSEGHSFFDREGKGDITQFLASYLEQYRQTVGKGYINLPLGNHDLSRLNIERSD
                     DDLEIIFAFSLTMPGIPFIYYGNEIGMRQLYDLPNVEGAYPPRAGARTPMQWSQGKNL
                     GFSTANPEKLYLPVDPAADAPNVADQQDDPNSLLNRVKNLIQLRKNSKALRAYAEFVP
                     VYAKKGEYPFIYARACEDETVLVILNPANKAATARFRLKPVFSQFELLAGTESELKLE
                     DKYYQLTIPGGSYSIYQLGN"
     gene            complement(7498..9459)
                     /locus_tag="PLI55_06500"
     CDS             complement(7498..9459)
                     /locus_tag="PLI55_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012486478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HPB00521.1"
                     /translation="MISAKIIRSFVTLLISLTMIQTCSSNKPKNQYLKSPDGNITVRF
                     EISPAGKIFYRVAYRNKEIVQASRMGIVREDADFSEGLKLDSVSASRPINFNYQMPQG
                     KKSHYAYNGNEKRFYLTNHVNQKIDVVFRASNDGVAFRYIFPGQSDEPKTILREISEF
                     HFSPRTKAFIQPMAMAKSGWCQTNPSYEEIYQQNVAIDKLPFNEPGWVFPALFEDGDF
                     WMLISETAPDRNYCGCRLQQDSTSHIFYIDFPSDLETFPGGIWLPTSPLPWSTPWRII
                     TIGKGLKNIVESTLGTDLAEPARLKEIDYVKPGRSSWSWVLLKDDSTVFEVQKRFIDY
                     AAEMNWEYCLIDADWDWKIGYDKVAELAAYAQTKGVGILLWYNSSGDWNSTTYSPKSK
                     LLTHSDRLREFARLKKMGIRGIKVDFFGGDGQSMMTYYQDIFEDAAQFGLVVNCHGAT
                     LPRGWQRTYPNLLTMEAVRGFEFITFDQADADLEPTHACLLPFTRNVFDPMDFTPVCF
                     SEIPNIRRITTNGFELATAVIFWSGIQHFAEIPAGMACVPDYIKNFMREVPVSWDETY
                     FIDGFPGKFVVLARKSGQTWYLAGINGADVERQLKLNCNFLDEDHTGQLITDAADGRS
                     FMMTEIHVSPQSLLSIKLEKYGGFVIKFD"
     gene            complement(9549..11990)
                     /locus_tag="PLI55_06505"
     CDS             complement(9549..11990)
                     /locus_tag="PLI55_06505"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HPB00522.1"
                     /translation="MFEKIKIVPKILLMTISLGLFLACLPTCGQRIRLPERGSVSWLP
                     GSKWEEALLTGNGTIGAMVIGRPHDETIILNHALLYLPASDRLPPINQASRLDEIRQL
                     LLQGKYAEAAKIPVEIGETEGYQGTHWTDPYMPALNIRLNMKAANVSDYARAVDFTTG
                     EAMVFWLDEGGRFERKLFVSRADNCIVMSIKGSGKINTSLEFAMHPVEWSQQEMVNGA
                     IEFTEIGAKDCWLTYRCKFKRHYDDNPQGYEAVGRVILKGGNLSSEGNQLVIQNADEI
                     LLLVRLEPNFDWENSRVSELQSALQNQPTTYKTLLERHVNIHGNLYNRVKLDLGGRKL
                     KTLQSEVLVARAREEVTPPMIEKFFDAARYNILCATGTRPPNLQGIWNGTWSPPWSSD
                     FTNDGNAEVAISSLLCGNLPELMLAYFNYHEKLLPFYEDNARRLYGCRGIHIPSRTSS
                     HGWDIHFGETWCLTFWTAGAGWVSSFFYDYFLYTGDTTFLKERAYPFMKAAAQFYEDF
                     LTIGENGKYVFNPSYSPENNPANSNSQACINATMDIMVAKQLLRNCIQAAKILRVDES
                     QAIKWQNMLSLMPEYEINEDGALREWLWPGLEDNYNHRHVSHLYGLYEMLDPDIAGNP
                     ELIKSAKNAIAERMKIRRRENGGAMVFGLVQMALVAANLGDAEMAEELINWMASYYWS
                     NSLASLHDPGNLFNMDLSGGFPAAVIRTLVYSEPGMIKLLPALPNSWPAGSISGVLVR
                     GQIEIKELKWRENQILVTLQSRVNQNLELRMPRPVGRILGPDRDLITRLPNDDRNFQI
                     FLPADSDVTVKFVCQ"
     gene            complement(12048..13718)
                     /locus_tag="PLI55_06510"
     CDS             complement(12048..13718)
                     /locus_tag="PLI55_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase"
                     /protein_id="HPB00523.1"
                     /translation="MRRIWRSIIVLGFGIRILVGAEPPQGFFLYDFQPKKATIPPFRE
                     VTLTQSRSTVTVEIDIGDTLTKVPKYIYGHNANVYMTQMVNEPTLIGYIKQLAPQILR
                     YPGGNLSNLFFWNNQPGQLPEDVPDPLYGGVEKPYREEFWFGRNDTAITLSVDNYYKM
                     LEMTNCTGIICVNYSYARYGKGENPVATAAKYATDWVRYDNGRTKFWEIGNENFGNWQ
                     AGYKINTADNKDGQPEIITGELYGKHFKVFADSMRAAARSIGVEIKLGAGLLESAQDW
                     HTPTEKTWNSGFFKQAGDAADFFVIHSYYTPWNENSSVGTILNSAIPVTQEMMTFMQE
                     TARINEVKMKPVGLTEWNIFAVGSKQQVSYINGIHAVLVLGELIKDKYGLACRWNLAN
                     NWNDGNDHGMFSQGGEPNVPRWNPRPVFYYMTYFKQFCGDHLVNTNVVGDNDVVAYAS
                     SFSSGEAGIILVNKGNSPKSVRLEFANFQPGRRCYWYLLTGGKDNGDFSLQVFINGVG
                     PELPAGGPANFEKIKAYSTRITKPLVFDLPPYSVLYLLVESDRKIPVK"
     gene            complement(13855..14085)
                     /locus_tag="PLI55_06515"
     CDS             complement(13855..14085)
                     /locus_tag="PLI55_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00524.1"
                     /translation="MNNKKSIRICPNCGEYLDRIWFTAIMTEEWSWNGEGYSECTAHH
                     SLGTDPQEEVLCPYCEAVVGTGYDFGFGKGCN"
BASE COUNT         4091 a         3436 c         2837 g         4049 t
ORIGIN      
        1 gtcccactaa ccgcaactaa accttcaatg ccaccagcct cgtcagtcac ccatggtcca
       61 ctaaatccac taccggctga acctaacccc gctacggaag ttgctggata actaaaagac
      121 tcacgagcaa ccgttccggt tggtgtttca gcggttaaat cagcaaaaga ggaagcgacc
      181 gtgatttcgt cgaagactaa cctgacatca gctccatcgc caccaaattc caatgcaata
      241 tgatcaaatc cttctggcat cgaagaatta cgctttacaa tagcatcatt ggtatccggt
      301 tctacgctcg gatccgggtc tatccacata taagtccggt cagaaccagt gccgcctgac
      361 atcttcacca tcgttactat ccataccggt ccaactgtaa ttggagtcgc agatacatcg
      421 tcaccttctc caacccaacc acttccacag gtaaataccg gcggagtagg accaccacct
      481 gctttaccta tcgctaacaa ttcaccacta cctttataca atttcaccat aaaataggtg
      541 ttctcaatcg gtagaggctc cttaatgtcc aacagataac tgacccagta ttttttgcca
      601 gcctcattcg gccaagctcc atccaagggc ctcttgtaac gattatgatc agcccaggca
      661 ttactcttaa ctacctgcag atgaagcgta tcatgtggaa tctcatagtt aagatcagca
      721 taagcaaatc tcgtcccact aaccgcaact aaaccttcaa tgccaccagc ctcgtcagtc
      781 acccatgatc cactaaatcc attactcgca gttcccagcc ccgccagagt agtcgctgga
      841 taactaaagg attcacgtac tagtgactga ggcattgcaa ttgagcttag cgccataatc
      901 aaaacgacac taataacaac agttctcatt cgcataattt ctctcctttg tttgtttttt
      961 taatgcaatt aaacgttaga aacaacattc ttagtgattt gtagaaccca cacctccttt
     1021 cgtctttttt aattatcatg aaaaaacctc actcttagat tgagtgttta tacaatatca
     1081 caaaaaaaac gatgatgaat atgatacaaa tgacctcaat cagaatccat ttccaacgat
     1141 tacgattttc ggcgctttca actgggtctg gtttgtttaa tttctgtttc atttgaaact
     1201 aaagtaaagt gtttaaaaag aaaaatcatc tcaaaatctg gtaattaggt tgcataccga
     1261 ttttaaaggt gaaatttgat tttaaataaa gaatggaagg gctaagaaag ctcggattcg
     1321 ggacggattt gctggggtgt tcgtccaaat tgcttgcgaa agcatttagt gaaataggaa
     1381 gggtccttga aaccaatttg gtaacaaact tgagatggcg aatgcccttt ttccagtaat
     1441 tgcatagctc tctgcaagcg gtaattacgg ataaattcac tgggagtctg gttaatcaaa
     1501 ccttgcattt tacgataaag ctgggcgcga ctcaagccga cgtgatagct aagatcatca
     1561 acggaaaaac cgctatccga taaatattgt tcgacaacct caaccgttct ctgcagaaat
     1621 tgttcatcag gtgaactgac gttaatttct ttcgggttca tataaaccat atggccaaaa
     1681 cgttctcgga tttttcggcg agtgttcagt aaattttcga ttcgaatccg cagaagatct
     1741 gggttgaaag gtttggtgat atagtcatcc gcacctgcta ataaaccttc tttgcgcttt
     1801 tcttccgaag tttgagccgt tagcaggatt atgggaatat ggctggtacg ctggtcattt
     1861 ttacatctat ggcaaaattc atttccatcc atcacctgca ttaaaacatc gcaaataatc
     1921 aaatccggaa ttcgatctac cgctaattca tagccagtta aaccgtcggc ggcttgaatt
     1981 accgaataat agctctcgag ttccatcgcc aggtaatgtc gcaggtcatt atcgtcttca
     2041 actattaaga taaggggctt agctttatca ctatttttca tagacaattt ggactcggta
     2101 agttcattcg aaattttcag gttttcatcc ggaattaact cagccatctc tccaatatag
     2161 tcttgttcac caacctcttg ctcgccagtt tttaacgctt cagccattgt tctcatcggt
     2221 ataatcaaag agaaggtact acctttgccc ggcgtgcttt ctacgatgat tctgcccccg
     2281 tgcatctcga taaaatctct gctaattgaa aggccaatac ccgtgccgtg cattttcctg
     2341 gtctccggct ggcgcacctg gtaaaaggga tcaaaaatat gttcaatctg atctggcgga
     2401 atgccaatgc cggtatcacg tacttgaacg cagatcgaac gctccgcttc ttctcgtttt
     2461 gaataacttt ccatatttgg cggagctaaa aagagggaaa cctctaccat gcccccgggg
     2521 ttggtaaatt ttagtgcatt ggcaattacg ttataaaggg ctttttcaat tttatccgga
     2581 tcaaagtaga aatattgttc ttccagttcg gttttgaata tataccgaat atttctctgt
     2641 tcagcctgga gatgaaaaac cgaaaagatg gctctaacaa atgagataaa ctctttttgc
     2701 tgtaattcca gtttcatact accggtatca agtttacggg catccataag ctgattgacg
     2761 agtctcagca accggcgggc gttttggagc accagccgat tatagacatc tcgtttttcc
     2821 tgggacaatt tattgccgag atgaagcatt ctttcgaccg gcccgataat gagagtaagc
     2881 ggagtccgga actcgtgcga tatgtgagta aaaaatcgta acttaagttg atccaattca
     2941 tgagtctttt cggcctctaa ccgtctcatt tcagcctgag cccgattgcg ttcgcgcacc
     3001 tggacagcca tacgaattaa caaaatggca ccgagaatca aaccgatata gataatcaac
     3061 atccaattag tttgccagaa aggcggagtg attttaagat gaatggctgt gccggattca
     3121 ttccaaaccc cgtaattatt ggatgcttta accctaaaag tataattgcc aggcggcaag
     3181 ttagtataag tggcgaaccg gcgattggca tcggtgcggc gccagtcttg gtcgaaaccc
     3241 tccattatat aagcatattg attcttttgc gggcagctat aatcgagagc ggcaaactcg
     3301 aaagaaaaga cattttcgcg gtaagaaagg atgatatttt ccgtaataaa aatcgaacta
     3361 tctaacttga tctttccctg cgtattcgga ctaattgttt cattaaaaat ttgaaactgg
     3421 gtaatcacca ctggcggaat gaacggattg taactaatac tgcccggttg aaaaacattg
     3481 aatccattta ttccaccaaa ataaagcata ccgttgctgg cgcgtaaaac cgattcacga
     3541 taaaagttgt cttgcccgaa tccatcatta cgatcaaaat tgtgaatttc atcattggcg
     3601 ggattcagac gagatatacc atgattggtt cccatccaga tgaaaccgtc tccatcttca
     3661 ataagactct ctatgatgtt atcgggcaga ccatggcgtt gatcataccg cccaaagtgc
     3721 gcaatgcccg aatccgcttg aaaatattgg acataattca acccacctcc cattgtacca
     3781 atccacagag tcccatcctg gctgacaaac aaagtattga tattctcatg tcccagactc
     3841 agcgaatcgg tatcatcagg gcggaaaaac agcatttttt cattgttcgg attgaagaat
     3901 accagccctt taccataggt tcccagccag agtccgccgg atgtatcttc cgcaatagaa
     3961 gtgacaacgt tcagacggtc attaatccga tctatgggat attggctaaa agtctgcccg
     4021 tcagaattca atttagatag accgccgccc caggtgccta cccataaggt attagattta
     4081 tccaaataga gagcgtaaac ggcattttct ccaagagagg aaggattttg ctcatcatgc
     4141 cggaaatgct taaactgacc acttttgaaa tcgtaacgat ccagaccact accgtcagta
     4201 ccaatccaga gataatctct attacccgtc aaacataaaa tcgcatcgga tccgagacta
     4261 ttattctgtt cgtcttttcg gtaaactcga aagtggtttg caactggatc gaagcgattc
     4321 agacctccga aacgagtgcc aatccaaatt ttcccatatt gatcttcaaa aaatgaggta
     4381 acatgattat tcgagagact attagcccca gaaagtccaa aacggtaatg caaaatatga
     4441 ctctgtttcg tatcgcattt attgattccg ccattttcat ttccgatcca aaccacacca
     4501 gttcgatcca gataaaccgt ccagagcgca ttggattgga tgctcgtttc gtcaagaggt
     4561 tcacaaagat aatgttggat tccttcaatg ccggcggtgg taacatttct cgggatttga
     4621 ggtatgacga ataatccggc gtcataagta gacatccaca tccggtctga ttgatcaatt
     4681 aataattttg tgattatttt agtagtaaac ggttttttac ctttgcaaaa aataggtact
     4741 ttgcggaatc tttgtgcatt gacatcaaat cgttgcagtc cgtcacccca ggtgccaatc
     4801 cagaggtttc cccatcgatc ctcgccgata gtaataacgg cgctggtgcc agccttgggt
     4861 gattcagtag tttcgtgacg ataacgagta aacgattgag ttatgcgatt aaagcgataa
     4921 acaccttctt tttcggtcgc aatccagagc tgatcgcggc tatcattata aatgtcatga
     4981 ataataaaat cacccaagcc atttggatta ttcggatcgg gaagatagtt ctggaaagta
     5041 tcaacccgag gatcataaca acttagcccg ccctctgtgc cgatccagat ttgcccataa
     5101 ttatcctcgg caatacaacg tatgcgatta tcaactaacg aattgggatt attaggatta
     5161 tgacgaattc taataaaaca atcccgtgcc cgatcgtaac gattcaagcc gtcgagagtg
     5221 ccaatccaca aaattccacg cctatcttca tataaggcgt tgattacatt atggcaaagg
     5281 ctggtcgtat ctcctaaaat cggtcggtaa gccgtgaaat tatatccgtc gtatctatct
     5341 aaaccattta atgttccaaa ccacataaat ccctgccgat cctgaatgat ctcgataatg
     5401 gaattgtggg ataaaccgtc ttgagtagaa agatgctcaa aacgcaaagt cggtaatccg
     5461 gcactgaagg cattgctact taaagccagc agaattggcg caattgtcaa tcctgatatt
     5521 tttctcagaa aactacccat aagattcaat gaccatggtg atcatttatt aattcaattt
     5581 aatctcggga aattaactcc attttagttc cccaactgat aaatagaata actgcctccc
     5641 ggaattgtca gctggtaata tttgtcttcc aacttgagtt ctgactccgt tccagctaat
     5701 aattcaaact gagaaaacac cggtttgaga cggaatcggg cggtcgcggc tttattagcc
     5761 ggatttaata taaccagcac agtttcgtcc tcacaagccc gggcgtaaat aaaagggtat
     5821 tctccttttt tggcataaac cggtacaaat tcggcatagg cgcgtaaggc tttcgaattc
     5881 ttgcgtaatt ggatgaggtt ttttactcga tttaacaatg aattaggatc atcctgttga
     5941 tcggctacat tgggtgcatc agcggcagga tcaaccggca ggtataattt ttctggatta
     6001 gcggtagaaa agcccaaatt tttaccttga gaccattgca ttggagtccg tgccccagct
     6061 cgcgggggat aggcgccctc gacattcggt aaatcataaa gctggcgcat tccaatttca
     6121 ttgccataat agataaatgg aatcccaggc atcgtcagtg aaaaagcaaa aatgatctcc
     6181 agatcatcgt cagaacgttc gatatttaac cgcgataggt cgtgatttcc cagtggcaga
     6241 ttaatgtagc ccttgccaac tgtctggcga tattgctcta aataacttgc caaaaattga
     6301 gtaatatcac ccttgccttc tcgatcaaaa aaactatgcc cttcggaatg gccattcata
     6361 attcgccaac tctctttctg cagtagatcg ttgtaacctg aaaaccagtg aaaaaaatcg
     6421 gcgtggaaac cacctccatc cagagccgcc accggatatg accactctgc taccataaag
     6481 gcttgaggat attcttcgtc tatgatcttt ctgattttcc gccagaaaat ttttgtgcct
     6541 tcatctcgat tagcatattc gggatcacct tcgacgatat tggcattctt gaccaatgcc
     6601 cccgccatat cggctcgaaa accgtccgca cccatatcca tccagaaacg caaaaccttc
     6661 tgcaattcct cctgaagtgc cagcacatcc ggatggtcaa tcggtaacat ccaatcgctc
     6721 tccggtttga cgaatccgaa attcaaagct ggctgactcc agtaaaaatt gcgcataaat
     6781 tggccattcc gtcggctata gcctttaata aaagcatcgc gataggcttc cggcggatca
     6841 atccaggtat tattggtcca gacgtaccag ttcgaatatt tattgggttc ctgacgagca
     6901 gattcttgaa accaggggtg ctcgatgctg gtgtagctga tgacataatc gaaaaggacc
     6961 tttagtccgc gtttttcggc ttccaaaaat aaacgccggg catcctgatt ggtaccgtaa
     7021 cgcggcgcta cttggtaata atcggaaata tcatacccgg cgtcattaaa aggtgatgcg
     7081 aaaaatggat taacccagaa tccgtcaacg ccgagactct gcacatagtc caatttttca
     7141 ataatcccat tcaaatcacc aatgccgtcg ccgttggaat cgtaaaatgt ctgcggataa
     7201 atttgataaa atacggcatt atgcaaccat tcaggaccag gcggcacaat tgtttgcccg
     7261 gttatttctg ccgaatcaat agaaatatcc actgcggact tatcttcact acatcctacg
     7321 attagcatta aggtcaaaat aagtgtcacc atccgacgat gcataaattc ctccgatcat
     7381 ttcaggagtt gatcaaactc cccgcacttt cacctgctct tttaccatca attataaata
     7441 aatctgccaa taatattcct cttgaaaaac agttcaatat taggatttat ccgattatca
     7501 atcaaatttt attacaaagc caccgtattt ttccagctta atagatagaa ggctttgagg
     7561 agaaacatga atttctgtca tcataaaaga acgcccatcg gccgcgtcag taattaattg
     7621 acctgtgtgg tcttcatcta aaaagttaca atttaatttc agctgtcttt cgacatcggc
     7681 gccatttatt ccagctaaat accaggtttg ccctgacttt cttgccagca ctacaaattt
     7741 gcccggaaaa ccgtcaataa aataagtctc atcccagcta accgggactt ctctcataaa
     7801 atttttgata taatccggaa cacacgccat accggccgga atttccgcaa aatgctgaat
     7861 tcccgaccag aatataaccg cagtagccaa ttcaaagcca ttcgtggtta tccgtcgaat
     7921 atttggtatt tccgaaaaac aaactggggt aaaatccatc ggatcgaaaa cattacgcgt
     7981 aaatggcaaa aggcaagcgt gggtcggctc tagatcggca tcagcctgat cgaaagttat
     8041 aaactcaaaa ccgcgcaccg cctccatcgt cagcagattg ggatacgttc tctgccaacc
     8101 gcgtggtaag gtagcgccat gacaattcac caccagaccg aactgcgccg catcttcaaa
     8161 aatgtcctga tagtaggtca tcattgattg cccatcacca ccgaagaaat ccactttaat
     8221 cccgcggatt cccatctttt tgaggcgtgc gaattcccta aggcgatcgc tatgagtcag
     8281 caatttactt ttcgggctgt aagttgtgct attccaatcg cccgaagaat tataccagag
     8341 cagaattccg acgcctttgg tttgggcgta agccgccagc tcagcgactt tatcataacc
     8401 gattttccaa tcccaatcgg catctatcaa acaatattcc caattcattt cagcggcata
     8461 atcaataaac cttttttgca cttcgaaaac tgtggaatca tctttaagaa gcacccagct
     8521 ccacgacgag cgacccggtt tgacatagtc aatctctttc aatcgagccg gttcggccag
     8581 gtccgttccg agagttgatt cgacaatatt tttcaaccct ttgccgatcg taataatccg
     8641 ccagggggtg ctccatggta gaggtgaagt cggtaaccaa atcccacccg gaaaagtttc
     8701 caaatccgat ggaaaatcaa tataaaaaat atgactcgtc gaatcctgtt gcaaccggca
     8761 tccgcagtaa ttgcgatcgg gcgcagtttc actaatcagc atccagaaat cgccatcctc
     8821 gaagagcgcc ggaaaaaccc agccgggttc gttaaatggc aatttatcta tcgcaacatt
     8881 ttgctgataa atttcctcat aggaaggatt ggtctggcac cagcccgatt tagccattgc
     8941 catcggttga atgaaagctt tagttcgagg tgaaaaatga aattcgctga tctcccttaa
     9001 gatggttttg ggttcatccg attgcccggg aaaaatgtaa cgaaaggcta ccccgtcatt
     9061 cgaggcgcga aaaaccacat ctattttttg attcacgtgg tttgtcagat aaaatctctt
     9121 ttcattgccg ttgtaagcat aatggctttt tttgccttgt ggcatttgat aattgaaatt
     9181 aatcgggcgg gaggctgaga ccgaatccag ctttagacct tcgctaaaat cagcatcttc
     9241 tcgaacaata cccattcggg aagcttgaac aatttccttg ttccgataag caacgcgata
     9301 gaaaattttc ccagccggcg atatttcaaa ccgaaccgta atattgccat ctggactttt
     9361 cagatattga tttttcggct tgttagagct acaagtttga atcatcgtta atgaaattaa
     9421 taatgtcaca aacgaacgaa tgattttcgc gctaatcatt tttctcctca tattaaatat
     9481 tttcaattat aaagtttgcc gctattttac cgcaattttg aaattcagat tcctatcaac
     9541 tataagattc attgacaaac aaatttaacg gtgacatcac tgtcagccgg cagaaagatc
     9601 tgaaagtttc tatcatcatt cggcaatcga gttattaagt ctcgatctgg gccaagaatc
     9661 cgcccaaccg gtcgcggcat cctcaattct aaattttgat tcacccgcga ttgtaaagtc
     9721 actaatatct gattctcccg ccatttcagt tcttttattt cgatttgtcc acgcaccaga
     9781 acgccactaa tacttccggc aggccaggaa ttaggcaaag ccggcagtaa tttgatcatt
     9841 ccgggctcag aatagaccag cgtgcgaatg acggcggctg ggaaaccgcc gcttaaatcc
     9901 atattgaata aattgcccgg atcatgcaga ctcgccaatg aattgctcca gtaatagctc
     9961 gccatccagt tgatcagttc ctccgccatc tcagcgtcac ccaaattggc ggcaaccaat
    10021 gccatttgca ccaagccaaa gaccattgcg ccgccatttt cccgccgtct gattttcatc
    10081 cgctcggcta tcgcattttt tgcagatttt ataagttcag gattaccggc aatatcagga
    10141 tcaagcattt catataatcc ataaagatgc gaaacgtgcc gatggttgta attgtcttcc
    10201 agtccaggcc atagccattc gcggagagcg ccgtcttcat tgatctcata ctccggcatt
    10261 aggcttagca tattttgcca tttaattgct tgggattcat caaccctaag aattttggcg
    10321 gcttgaatgc aattgcgcag aagctgtttg gcaaccataa tatccatcgt ggcattgatg
    10381 caggcttgcg aattggaatt agccggatta ttctccgggg agtaggacgg attgaaaaca
    10441 tatttgccat tctctcctat agtcagaaaa tcttcataaa attgcgccgc cgctttcata
    10501 aacgggtaag cccgctcttt caaaaaagtc gtatctccag tatataaaaa ataatcatag
    10561 aaaaaactgg agacccaacc agcccccgcc gtccagaaag tcaaacacca ggtttcgcca
    10621 aagtgaatat cccaaccgtg cgaactggtg cgggatggaa tatgaattcc gcgacaacca
    10681 tataaacggc gggcgttatc ttcataaaac ggcaataatt tttcgtgata attgaaataa
    10741 gcaagcatca attcgggcaa attgccacat agcaaactgg aaatagccac ctcggcattg
    10801 ccatcattcg taaaatccga tgaccaggga ggcgaccaag tgccattcca aattccctgt
    10861 aaattcggcg ggcgcgttcc agtggcgcaa agaatattat agcgcgcggc gtcgaagaat
    10921 ttctcgatca taggcggagt aacttcttca cgggctctgg caaccagaac ttcggattgc
    10981 agtgttttta gtttgcgacc gcccaaatcg agttttaccc gattatataa attgccgtga
    11041 atattcacat gacgttcgag gagtgttttg taagtcgtgg gctgattttg taaagctgat
    11101 tgcaactcac taactcggga attttcccaa tcaaaattcg gttccaaccg caccaaaagc
    11161 agaatttcat cggcattttg aatgaccagc tggtttcctt cactggataa atttccgcct
    11221 ttcagaatca ctcttccgac ggcttcataa ccctgcggat tgtcatcata atggcgtttg
    11281 aacttacagc ggtaagttag ccaacagtcc tttgctccaa tttcggtaaa ctcaatggcg
    11341 ccattgacca tttcctgttg actccattcg actgggtgca tagcaaattc caggctggtg
    11401 ttgattttac cgctaccttt gattgacatc actatgcaat tgtcggctcg cgaaacgaac
    11461 aatttgcgtt cgaaccgacc gccttcatca agccagaaaa ccatcgcttc accagttgta
    11521 aaatccactg ctcgagcata gtcgctgacg ttggcggctt tcatattcaa gcgaatattc
    11581 aacgccggca tataggggtc agtccagtga gttccctgat aaccttctgt ctccccgatt
    11641 tcgacgggaa tcttggcggc ttctgcatat tttccctgca ataaaagttg acggatttcg
    11701 tccaatcggc tggcttggtt aatcggcggt aagcgatcac tcgccgggag atagagtaaa
    11761 gcatgattta aaataatcgt ttcatcatgc ggccgcccga tgaccattgc cccgatcgtc
    11821 ccattgccgg tgagaagcgc ctcttcccat ttgcttcccg gtagccagct gacagagccg
    11881 cgttccggta acctgatgcg ctgtccacag gtcggtaaac aggctaaaaa taatcccagc
    11941 gaaattgtca ttagcaaaat ttttggcacg attttgatct tctcgaacat agtatttcta
    12001 tttttgatta atggtaaaaa atctaaggtg tttacagata gatagcatta ttttaccggg
    12061 atttttcggt cgctttccac taacagatat agaacagaat agggaggcaa atcgaaaacc
    12121 agtggcttgg tgattcgggt tgaataggct ttgattttct caaaattggc tggaccaccg
    12181 gctggtaatt ctgggccaac accatttatg aaaacttgca gtgaaaaatc accgttgtcc
    12241 ttgccgccag tcagcagata ccagtaacag cgccgtcccg gttgaaaatt agcaaattcc
    12301 agtcgcaccg atttgggaga atttcccttg ttgaccagaa ttatcccagc ttcgccggaa
    12361 ctgaagctcg aagcgtaggc aaccacatcg ttatcaccga cgacatttgt attgaccaga
    12421 tgatcgccac aaaattgctt aaagtaagtc atataataaa aaaccggtcg gggattccag
    12481 cgtggcacat tcggttcacc accctgcgaa aacatcccgt gatcattgcc atcattccag
    12541 ttattcgcta gattccagcg acaagccagg ccatatttat ccttaatcaa ttcacccagc
    12601 accaggacgg catggatgcc attaatatag ctgacctgct gtttcgaccc gacggcaaaa
    12661 atattccact cggtcaagcc caccggtttc atttttactt catttattcg ggcggtctct
    12721 tgcataaaag tcatcatctc ttgcgttacc ggtatggcgg aatttagaat tgtaccaact
    12781 gaagaatttt cattccaggg tgtataataa gaatgaatga caaagaaatc ggccgcatct
    12841 ccggcttgct taaaaaagcc ggaattccag gtcttttccg tcggtgtatg ccaatcctgc
    12901 gccgattcga gcaagccggc tcctagcttg atttccactc cgattgagcg agcggcggca
    12961 cgcattgaat cagcaaaaac tttgaaatgt tttccgtata attcgccggt aataatttcc
    13021 ggctgaccgt ctttattatc ggctgtattt attttatatc cagcctgcca attaccgaaa
    13081 ttctcgttgc cgatttccca gaatttcgtt cgaccattat catagcgcac ccagtcagtc
    13141 gcatatttag ccgcagtagc caccggattt tcacccttgc catatcgagc ataactgtaa
    13201 ttcacacaaa taattccggt gcaattcgtc atttccagca ttttatagta attatcaact
    13261 gaaagcgtga tcgcggtatc gttccgcccg aaccagaact cttcgcggta gggcttttcg
    13321 acaccgccat aaagcggatc cggcacatcc tccggcagtt ggccaggctg attattccag
    13381 aaaaatagat tactcaaatt tccacccgga tagcgcagga tttgcggcgc aagttgcttt
    13441 atgtaaccaa tcaaggttgg ttcattgacc atctgggtca tatagacatt ggcattatga
    13501 ccgtagatat atttcgggac cttggtcaac gtatcgccaa tatcaatttc aaccgtcacg
    13561 gtcgaacggg actgcgttaa cgtgacttcc ctgaaaggcg gtatagtggc tttcttcggc
    13621 tgaaaatcat atagaaaaaa gccctgcgga ggttctgctc cgactaaaat ccggattcca
    13681 aatcctaaaa caattatact acgccagatt cttcgcaact cgatttcccc ctataaaatt
    13741 atcggttttg tgtaactctt taaccaatca attcaagttc tatagaattt ctcgctacta
    13801 ctcatgataa tcttttaagt tatttcttct atagcatttc tttttcatat ctaactaatt
    13861 gcatcctttg ccaaatccga aatcataacc agtgccgaca actgcttcac aataggggca
    13921 gagaacttcc tcctgaggat cagtaccaag actatggtgt gcggtacatt cactatatcc
    13981 ttcaccattc cacgaccact cttcggtcat tatcgccgta aaccaaatac gatctaaata
    14041 ttcaccacaa ttgggacaaa ttctaataga ttttttatta ttcatttagt taatacctct
    14101 atatttaatt acttgctttg gaagcttaat atatttttct ttgacaattc tgcaagctgt
    14161 aaaaaagggc aaatgtcttt ttatctttcc tgccagtttc ttatcttcga aaaattctat
    14221 caggtgattt ggcatttttt ataactcctt ctgtttatgg gctaattttg gatactgttt
    14281 ccagtataga aaaagcccta ccgaaggtag aattttggcg agtgccgcaa gacacaaacc
    14341 ttttatattg actaataccg cgggtactct tcactaataa tactctttca ttaggctctt
    14401 tttatctcgg cag
//
LOCUS       DAOOMU010000056        14105 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4656_length_14105_cov_17.558790, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000056 DAOOMU010000000
VERSION     DAOOMU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 14105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14105
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4656_length_14105_cov_17.558790"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..2163)
                     /gene="fusA"
                     /locus_tag="PLI55_06520"
     CDS             complement(67..2163)
                     /gene="fusA"
                     /locus_tag="PLI55_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPB00525.1"
                     /translation="MKSENLSKVRNIGIMAHIDAGKTTTTERILFYTGKVYKIGEVNE
                     GSATMDWMEQERERGITITSAATTCYWNQHRINIIDTPGHVDFTAEVERSLRVLDGVV
                     AIFCAVGGVEPQSETVWRQADKYHIPRIAFVNKMDRTGADFFNVLDMIEKRLKAQPVA
                     VTLPLGSGELFTGIIDLITQQAILYDDNSLGSQYYNIEIPKDLKDIATQYRQKLIEEV
                     ANFDDVVFAKYLNNEDITPQELQNGLRKGTLENKIVPVLCGSAFRNKGIQPLLDAIVN
                     YLPSPFDMPPIEGVNLKGTPIKRYPRPDEPLSALIFKIMSDPHVGRLSFVRIYSGVIS
                     KGDMVFNSTVQKKERISRLLLMHANKREDREMLEAGEIGAIVGLKYSYTGHTLCDPKN
                     PIILESMQFPEPVIKISIEPKSKADEENLINALNKLADEDPTFEISTDEETGQILIAG
                     MGELHLEILVERLIREFNVKAHIGKPQVSYRETVVSVAEGEGRFIRQTGNKSQFAVVR
                     LRIEPDSASNEVRIENRIVPGTLPKEFVPAIEKGIRNHVKSGVLAGYPLQKVRVIITG
                     AEYREEESTELAFEVAAAMAFENALHKAELALLEPIMALEVVVTDEYLGDVLSDLNTR
                     RANIINVNKRGDANVIDAEAPLREMFGYATDLRSISQGRAVFTMKFSQFDYCDKRVQR
                     EIIEKARGFVPEFLKN"
     gene            complement(2184..2651)
                     /gene="rpsG"
                     /locus_tag="PLI55_06525"
     CDS             complement(2184..2651)
                     /gene="rpsG"
                     /locus_tag="PLI55_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPB00526.1"
                     /translation="MSRRKKVISREVLPDPKYGDIMVARFINYLLRKGKKSVAEASFY
                     DAIAQIEAKHKGNGLDVFKKAINNVSPIMEVKSRRIGGATYQVPIEVRPERRFALASR
                     WLITYAKARPGKSMAEKLAAEILAAANGEGNAIKKREDTHKMAEANKAFAHFR"
     gene            complement(2667..3038)
                     /gene="rpsL"
                     /locus_tag="PLI55_06530"
     CDS             complement(2667..3038)
                     /gene="rpsL"
                     /locus_tag="PLI55_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPB00527.1"
                     /translation="MPTINQLIRKGREVVSKKEKAPALRGSPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGEGHNLQEHSMVLIEGGRVKDLPGVRYHIIRGTL
                     DSSGVNDRKNSRSRYGTKRPS"
     gene            complement(3114..>4460)
                     /locus_tag="PLI55_06535"
     CDS             complement(3114..>4460)
                     /locus_tag="PLI55_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPB00528.1"
                     /translation="VLNLVDDNNRITSTYNIPYGAHVFVNEGQLVKAGHTLFRWDVYM
                     DQILALYSGKVRFVDLIENVTYNIEYDESGHQTMTVIESKDRSLQPRIDIVAQENGKE
                     VVYPGYILPVRAMLVVRDGDEVVKGDILAKKPKEIGKTSDITGGLPRVAELFEARRPK
                     DPAVISEIDGKVTFGKTEKGVREIIVTGIDGTTKKYKIPYGRYVLVNQGDEVRAGERL
                     CEGPVAPQDILAVQDPRKVQEYLVNEIQEVYRLQGVRINDKHIEVIVRQMMQKVRIED
                     PGDTNLLEKDRVNRHELIEENNRIKDYVVIVNAGDSDWDEGDVVSKKEFAKFNRLLKE
                     EGKNPAKARPARPAQFTEMLLGITRASLNTESFISAASFQETTRVLTDAAVAGKTDYL
                     RGLKENVIMGRLIPAGTGLRKYKDISVIEPEEEIEEWKKLEEQKKEKENELAALQK"
     assembly_gap    4461..4560
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4561..7016)
                     /gene="rpoC"
                     /locus_tag="PLI55_06540"
     CDS             complement(<4561..7016)
                     /gene="rpoC"
                     /locus_tag="PLI55_06540"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPB00529.1"
                     /translation="MNYTKPSTFRSEFESITIHLSSPETILNRSHGEVLKPETINYRS
                     YKPEKDGLFCEKIFGPVKDWECHCGKYKRIRYKGIICDRCGVEVARKKVRRERMGHIT
                     LAVPVVHIWFLKSIPSKLYYLLDIPTKNLEEIVYYEKYVVLNQGKSDLKYKTVITEEE
                     YFENMQAYGPKTELFDEEEAKNYFIAKTGGEAIRELLQKVDIESLVHSLKEELKIKKS
                     PTQKAEILKRLKVVMAFLNQEGKPINKPEWMVVTVLPVIPPDLRPLVPLEGGRFAASD
                     LNDLYRRVIIRNNRLKQLIEIKAPDVILRNEKRMLQEAVDSLFDNSRRRTEVRSGTRR
                     PLKSLSDMLRGKEGRFRQNLLGKRVDYSGRSVIVVGPDLRLNECGLPKDMAIELFKPH
                     IIHALLRRRLAKTPKAAKTMVERKDAEVYRVLEYVVKDYPVLLNRAPTLHRLGIQAFQ
                     PKLIEGKAIQIHPLVCAAFNADFDGDQMAVHIPLTFEAQIECRLLALSSHNILHPAHG
                     HPIAIPSQDMVLGVYYITRQKEGARGAGLVFSSPEEAIYAYEIGEVDLHAKVKIRIDG
                     KIIETTVGRALFNSLLPPGMGYYNELISKRSLEKIVGIVFQEAGNYETVKFLDNLKNI
                     GFKYATRSGVSISISDVLIPEEKEKILQESFERVEDIQRKFRKGILTEGERYNKVIDN
                     WTHTTNRLSEVMLEHMATDRQGFNPVYMMADSGARGSGEQIKQLAGMRGLMAKPQKSM
                     TGQVGEIIETPITSNFKEGLTVHEYFISTHGARKGLADTALKTADAGYLTRRLVDVAH
                     DVVISEEDCQTINGIEVTAL"
     gene            complement(7044..10826)
                     /gene="rpoB"
                     /locus_tag="PLI55_06545"
     CDS             complement(7044..10826)
                     /gene="rpoB"
                     /locus_tag="PLI55_06545"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPB00530.1"
                     /translation="MSNTLPLEKERKSFAKVKIVQKMPNLLEVQLKSFDEFLQKDTMP
                     LERKNIGLESAFRNVFPVEDTHGNYLLEYKYYTIGEPRYTIKECLERGVSYTVPLKVR
                     LVLHISEEGISKGEYQAGIEQDIFFGNVPFMTEKGTFIINGAERAIVSQLQRSPGVFF
                     DETEHTNGMKIYSARVIPFRGSWIDFVIDTRDILSAIIDRRRKFPATILLRAFGYPTN
                     EDIVNLFGIGKRFSLDGDSKELLGKMLAENVIDPETGEVISEIKEEIVVDKDFLKKCK
                     QHGITSLLVFTTDDEIERNIFLNTFRKDTQTNDEESALYFLYRSLRTGEPPNLDIAKK
                     FVERLFFSPKKYDLGQVGRYRINKKFNLNVPLEECVLTRDDIVEIMKRIVDMKKGNLG
                     PDDIDHLGNRRVRNVGEQLTNQFNLAFTRMARTIRERMNLRESENLTPQDLINSRIIT
                     SVINSFFGTSQLSQFMDQTNPLAEVTHKRRVSSLGPGGLSRERAGFEVRDVHYTHYGR
                     LCPIETPEGPNIGLIASLATYAYVNDLGFIETPYRRVEKVNGIATVTDKVEYLSADDE
                     DREIIAQANEPLDEQGHFINSRVRARVRGEFPIVTPDEVKYMDVSPEQIVSVSAALIP
                     FLEHDDANRALMGSNMQRQSVPLLKPERPIVGTGLEGVVARDSQSVVLAPVNGIVEEV
                     TADHIVIRTDTENELYLDENQGKVRIELAKFLRTNQDTCINQKPLIKEGDRVKAGDAI
                     ADGCATNDGELALGRNVLVAFMPWRGYNFEDAIVLNERLVYDDVFTSIHIKEFELEVR
                     DTKRGNEELTREIPNVSEEATKNLDANGIIRVGAEVEPNDILIGKVTPKGETDPTPEE
                     KLLRAIFGEKASDVKDASKRADPGIRGVAVDTKLFTRKSKVSRMEEKRQIDEETKRSV
                     QKHAYLKGRRDLLLKNILKGATSATIKNLTNGKVAIKSGIQFNDEILAQLDFDLLSLE
                     TPWTNDPEINEKVEKIVKSYFILSQRIDEELEQTIYKIKVGDDLQPGVLQLAKVYIAS
                     KRKVQVGDKMAGRHGNKGVVSIIVPKEDMPFLEDGTPVDIILNPMGVPSRMNLGQLYE
                     TMLGWAGRELGVYYETPVFNGASEQEVQEELRKAGLPEIGKVSLIDGQTGEQFDNPVT
                     VGYIYMMKLSHLVEDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAA
                     YTLQEILTVKSDDVDGRSKVYNAIVKGENFSDFGVPESFNVLLKELQGLCIEMDIN"
     gene            complement(10932..11324)
                     /gene="rplL"
                     /locus_tag="PLI55_06550"
     CDS             complement(10932..11324)
                     /gene="rplL"
                     /locus_tag="PLI55_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPB00531.1"
                     /translation="MANITRADVLEYLEKANMLEISELIKDIEKKFDVKAAAPVAVAA
                     TVAQPGATAEAEAVEEQTEFNVILKEVGPNKINVIKVVRAATNLGLKEAKDLVDGAPS
                     TVKEGISKEEAAELKKQLEEAGAKVEVK"
     gene            complement(11354..11881)
                     /gene="rplJ"
                     /locus_tag="PLI55_06555"
     CDS             complement(11354..11881)
                     /gene="rplJ"
                     /locus_tag="PLI55_06555"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPB00532.1"
                     /translation="MPNQKNIQSLETISEKFQKARGIYFTNYKGLNVKEITDLRKELY
                     AADVEYKVVKKTLTKMAAKNSGLDSIEPLLDGQTALAISYTDPAIPGKVINTFIKKNK
                     LDKLVITGCIYDGVLFGADQVEKVINLPSRRELLASLLATLNAPMSQLTMVLRATLNQ
                     LVGTLNSLNEQKKQS"
     gene            complement(11896..12585)
                     /gene="rplA"
                     /locus_tag="PLI55_06560"
     CDS             complement(11896..12585)
                     /gene="rplA"
                     /locus_tag="PLI55_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPB00533.1"
                     /translation="MNISKRVEQNSKKVDRKKEYPLREALELLKNCNGAKFDETVEAH
                     VNLGVDPRHADQNIRGTVTYPHGTGRKVRVLVLTKSKVDEALQAGADYAGLEEYLEKI
                     QNGWTDVDVIIATPDVMGQVGKLGKILGPKGLMPNPKSGTVTNDVAAAVKEIKAGRVE
                     IRVDKTGIVHTIVGKKSFPIEHLEENVQTLISTLMRLRPPSVKGAYIQKVTLTTTMGP
                     GVRVDKSSIIA"
     gene            complement(12600..13025)
                     /gene="rplK"
                     /locus_tag="PLI55_06565"
     CDS             complement(12600..13025)
                     /gene="rplK"
                     /locus_tag="PLI55_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPB00534.1"
                     /translation="MAKKVLATIKLQIEAGKANPSPPVGPALGQHSVNIVEFCKAFNA
                     RTQDQAGYIIPVVITVYSDRSFTFITKTPPASVLLLKAAKVEKGSGEPNKSKIGSVTM
                     DQVMEIAKIKMPDLNAHTVEKAAEMIKGTARSMGISINE"
     gene            complement(13033..13560)
                     /gene="nusG"
                     /locus_tag="PLI55_06570"
     CDS             complement(13033..13560)
                     /gene="nusG"
                     /locus_tag="PLI55_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPB00535.1"
                     /translation="MEWYVLRTYSGKEKKVREAILIEAEHAGMKDIIKDVLVPSENIM
                     EMREGKKRVRNKVFFPGYILVNMDITKESQYLIENIPGVMNFVGAAGKPQPLKEDEVK
                     RVMGEVEKKDGREVLAVPFKMGDPVKVIDGPFIDFRGVVQEVNEDKRKLKVSVSIFGR
                     PTPVELDFLQVELEK"
     gene            complement(13571..13753)
                     /gene="secE"
                     /locus_tag="PLI55_06575"
     CDS             complement(13571..13753)
                     /gene="secE"
                     /locus_tag="PLI55_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPB00536.1"
                     /translation="MIKRIREFFEGVVFEMKKVSWPTWSELKSSTAVVLIFSLILSVF
                     LFVVDLILSKIMNVIF"
     gene            complement(13802..13874)
                     /locus_tag="PLI55_06580"
     tRNA            complement(13802..13874)
                     /locus_tag="PLI55_06580"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13839..13841),aa:Trp,seq:cca)
     gene            complement(13886..14035)
                     /gene="rpmG"
                     /locus_tag="PLI55_06585"
     CDS             complement(13886..14035)
                     /gene="rpmG"
                     /locus_tag="PLI55_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPB00537.1"
                     /translation="MRELITLECTVCKRRNYSSDKNKKLQPNRVEYKKYCRWCKKHTV
                     HKETR"
BASE COUNT         3693 a         3270 c         2751 g         4291 t
ORIGIN      
        1 caatcgtacc aatgttgagg tgcggcttcg tccgcttaaa ttgttgcttt gccatatttc
       61 agctccttaa ttttttaaaa actcagggac aaaacccctg gctttttcaa taatttctct
      121 ttgaactctt ttatcacagt aatcgaattg ggaaaatttc atagtgaaaa ccgctcgccc
      181 ttgtgaaatt gacctcaggt cagtggcgta gccaaacatc tcgcgcaagg gggcttcagc
      241 gtcaattaca ttggcatcgc ctcttttatt cacattgatt atattagctc gccgtgtatt
      301 cagatcacta agtacatcac cgagatattc atccgttacc accacttcca gcgccataat
      361 cggctcaagc aaagccagtt cggctttatg tagggcattc tcaaatgcca tagccgcggc
      421 tacttcaaaa gccagttcgg tggattcttc ttcacgatat tccgcaccag tgataataac
      481 acgcactttt tgtaatgggt atccggctag taccccgctt tttacatgat tgcgtatacc
      541 tttttcaata gccgggacaa attcttttgg taaagtaccc ggaacgattc ggttttcgat
      601 ccgaacttca ttgcttgcag aatccggctc aattctcaat cttacaacag caaattggct
      661 tttattcccg gtttgacgaa tgaatcgacc ttctccctct gcgacactca cgaccgtttc
      721 gcgatatgaa acctgcggct tgccgatgtg ggctttgaca ttaaactccc gaatcaggcg
      781 ttccaccaga atttcgagat gtaattctcc cataccggca atgagaatct ggccagtttc
      841 ttcatcggtt gaaatctcaa aggttgggtc ttcgtctgcc aatttattca atgcattaat
      901 caaattttcc tcatcagcct tacttttcgg ttcgatagaa attttgataa cgggctctgg
      961 aaattgcatt gattcgagaa taatagggtt tttaggatca caaagagtat gcccggtata
     1021 actgtatttt aggccgacaa ttgccccgat ttcacccgct tccaacatct cacggtcttc
     1081 acgtttgtta gcatgcataa gaagtaaacg actgatgcgt tcttttttct gtacggtcga
     1141 gttaaaaacc atatcaccct tacttatgac gccgctataa attcggacaa aacttaatct
     1201 gccaacatgg ggatcggaca taattttaaa aatcagggca cttagcggtt catccggacg
     1261 tggatagcgt ttaattggcg tgccttttaa attgaccccc tcaataggag gcatatcaaa
     1321 aggtgatggt aaataattaa caattgcgtc cagcaaaggt tgaattcctt tatttcggaa
     1381 agccgaaccg catagtaccg gaacgatttt attctcgaga gtaccctttc ggagaccatt
     1441 ctgtagttcc tgcggagtaa tatcttcgtt atttaaatat ttggcaaaaa ctacgtcgtc
     1501 aaaattggcg acttcttcga tcaatttctg gcggtattgg gtagcaatat cttttaaatc
     1561 tttaggaatc tcaatattgt aatattgact tccgagagaa ttatcatcat ataatatagc
     1621 ctgctgagta atcaaatcta ttattccggt aaacagttca ccagacccca atggcaaggt
     1681 gactgctacc ggctgggctt tcagccgttt ctcaatcata tccaggacgt taaagaaatc
     1741 ggcgcctgtg cgatccattt tattgacaaa ggcaattcgt ggaatgtgat atttatcagc
     1801 ttgccgccag acagtttcac tctggggttc gacaccacca actgcacaga aaattgcaac
     1861 gacgccgtcc aagacccgca gggagcgctc cacctcagct gtgaaatcta catgtcctgg
     1921 tgtatctata atatttatgc gatgttgatt ccagtagcag gttgtggcgg cggaagtaat
     1981 ggtaataccg cgttcacgtt cctgctccat ccagtccata gtggctgaac cttcgttcac
     2041 ttcgccgatt ttgtaaactt ttccagtgta aaacagaatg cgctcagttg tagtagtctt
     2101 accggcatca atgtgagcca ttatgccgat atttctgact ttgctgagat tttctgattt
     2161 cactctactt tatcctcaat agatcatctg aaatgggcaa aagccttgtt ggcttcagcc
     2221 attttgtggg tgtcttcgcg ttttttgata gcattacctt caccgttagc ggcggcgagt
     2281 atttccgcgg ctaacttttc tgccattgat tttcccggcc gagccttagc ataggtaatt
     2341 agccagcggg aagctagagc aaagcgtctt tcaggcctta cttcgatagg cacttgataa
     2401 gtagctccac cgattcgccg tgatttgacc tccataattg gtgaaacatt attaatagct
     2461 tttttaaaca catcaaggcc attaccttta tgcttggctt cgatttgagc aatggcgtca
     2521 taaaatgaag cctcggcgac ggatttttta ccttttctta aaaggtaatt aataaagcga
     2581 gctaccataa tgtcgccgta tttgggatcg ggtaggactt ctcgggatat aacttttttt
     2641 cgacgtgaca tacaatgttc tccctattag gacggacgtt ttgttccgta tctggagcga
     2701 ctgttcttgc gatcattgac accactggaa tccagggtac cgcggataat atgataacga
     2761 acacccggta aatccttaac acgaccgcct tcaattaaca ccatcgagtg ttcctggaga
     2821 ttatggccct cacctggaat ataagccgtt acttcgattc cgttggttaa acgtacacgc
     2881 gctacttttc ttaaagccga atttggcttc ttgggagtag ttgtataaac gcgggtacaa
     2941 acgcctcttt tctgagggga gccgcgtaag gcgggtgctt tttctttctt actgactact
     3001 tcgcgaccct tgcgaattag ctggttaata gtcggcataa tttctccata ataaaagcct
     3061 gtaaaaatac gacacttaaa aattttaaac aataaatttt aacaaccaga tatttatttt
     3121 tggagagcgg cgagctcatt ttctttctct ttcttttgtt cttctaattt tttccactct
     3181 tcgatttctt cttctggttc aatcacactg atatctttgt atttacgtaa accggttccg
     3241 gctggaatca agcgtcccat tatgacattt tctttcaaac cgcgtaggta atcggttttc
     3301 ccagctacgg cggcatcagt caaaacacgg gttgtttcct ggaaagaagc cgctgaaatg
     3361 aatgactcag tattaagtga ggcacgggtg atgccgagta acatttcggt aaactgggct
     3421 ggacgagccg ggcgagcctt agctggattc ttgccttctt ccttaagtaa tcggttgaat
     3481 ttggcgaatt cttttttgga aacgacatcg ccttcatccc aatcggaatc accggcattg
     3541 acgattacca cgtaatcctt gatacgattg ttttcttcaa taagttcatg gcgattaacg
     3601 cgatcttttt ccagcaaatt cgtatcgccg ggatcctcga tacgaacctt ctgcatcatt
     3661 tgacgaacaa tgacttcgat atgtttatca ttgatccgga caccctgcaa gcgatagact
     3721 tcctgtattt cgttaacgag atattcttga actttgcgtg gatcttgcac tgccagaata
     3781 tcctggggcg caactgggcc ttcgcataat cgctcaccag cccgaacctc atccccttga
     3841 ttgaccagaa cgtaacgacc atagggaatc ttatatttct tcgttgttcc gtcaataccg
     3901 gtaacgataa tttcgcgaac acctttctcg gttttaccga aagtgacttt accatcaatt
     3961 tcactgataa cagctggatc ttttggccgc cgggcttcaa acagttcagc tacccgtggc
     4021 agaccgccgg taatatcgct cgttttaccg atctctttcg gttttttggc taaaatgtct
     4081 cctttaacga cttcatcacc atctcgaacg actaacatag cacgcaccgg tagtatatac
     4141 ccgggataaa ccacttcttt tccattttcc tgagcaacga tatcaatacg tggttgtagc
     4201 gagcggtctt tggactcaat cacggtcata gtctggtgac cggattcatc atattcgata
     4261 ttataagtca cgttttcgat taaatctaca aaacgtactt tgccggaata gagggctaga
     4321 atctgatcca tataaacatc ccagcggaaa agggtatgac cggctttgac caattggcct
     4381 tcattgacaa aaacatgcgc accgtaggga atgttataag tagaagtaat ccggttgtta
     4441 tcatcaacca ggttcaagac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 ttcaaggcgg tgacttcgat accgttaatc gtctggcaat cttcctcgga aatcacaaca
     4621 tcatgagcta catctactaa tcggcgagtc aggtaaccgg cgtcagccgt cttcaaagcc
     4681 gtgtctgcca gtcccttgcg ggctccgtga gtagaaatga aatattcgtg aaccgtcaaa
     4741 ccttctttga aattggaagt aatcggcgtt tcaataattt caccgacttg accggtcata
     4801 gatttttggg gttttgccat tagaccgcgc attccagcca gctgcttaat ctgttcaccg
     4861 cttccgcgcg cacctgaatc agccatcata tagaccggat tgaagccctg ccgatcagtc
     4921 gccatatgtt caagcataac ttccgaaagg cggttggtag tgtgcgtcca gttatcaatt
     4981 actttgttat aacgctcacc ttcagtcaat attccctttc taaatttgcg ctgaatatct
     5041 tctactcttt caaatgattc ctgtagaatc ttctcctttt cctcaggaat taacacatct
     5101 gagatagaga tcgagactcc ggaacgagtg gcatatttaa agccgatatt tttcaaatta
     5161 tcgaggaact taactgtttc atagttgcca gcttcctgga atacaatacc aacgattttt
     5221 tcaagacttc ttttactgat taattcattg taatacccca ttccgggtgg caaaagacta
     5281 ttgaataaag cgcgaccgac agtcgtttcg ataattttac catcaattct aattttaacc
     5341 ttggcatgca aatctacttc gccgatttca taggcgtaaa ttgcttcttc cggtgaagaa
     5401 aaaaccagac cagcgccccg cgcaccttct ttctggcggg taatgtaata aacacctaaa
     5461 accatatctt gcgaaggaat agcaatcgga tgaccatgcg ctggatgtaa aatgttatga
     5521 ctggataacg ccaacagccg acattcgatc tgggcttcaa aggtaagtgg tatatggaca
     5581 gccatctggt cgccatcgaa gtcggcattg aatgccgcac aaactaacgg atgaatttga
     5641 atggctttgc cttcaattaa tttcggctga aaagcctgaa ttcctaaacg atgtagagtc
     5701 ggagcacggt tcagaagaac cgggtagtcc ttgaccacat attccagaac gcgataaact
     5761 tcggcatctt tgcgctccac catagtttta gcggctttgg gcgtcttggc tagccgacgg
     5821 cgaaggagag cgtgaattat atggggctta aagagttcga ttgccatatc tttaggcaaa
     5881 ccacattcat tgagacgtaa atctggtccg acgacgataa ccgaacgtcc actgtaatcc
     5941 acacgtttcc ccagtaaatt ctgacggaaa cgcccttcct tgcctctcag catatcgctc
     6001 agacttttga gcggacgacg cgtgccactg cgaacttctg ttcggcggcg actattatca
     6061 aataatgaat caactgcttc ctgaagcatc cgtttttcat tacgcaaaat gacatcggga
     6121 gccttgattt cgatcaattg ttttaaacga ttattgcgga taataacccg ccgataaaga
     6181 tcatttaggt cgctcgccgc gaatcgtcca ccttccagtg gaaccagcgg acgcaaatcc
     6241 ggcggaatga ccggcaaaac ggttaccacc atccattccg gtttatttat cggcttgcct
     6301 tcctgattca agaaagccat cacgactttc aggcgcttta gaatctcggc cttctgcgtc
     6361 ggtgactttt ttatttttaa ttcttctttc agactatgaa ctaggctttc tatatctact
     6421 ttttggagta gttcccgaat agcttcgccc ccggttttag caataaaata gttcttagct
     6481 tcttcttcat caaaaagctc ggtcttcgga ccataagcct gcatattctc aaaatactct
     6541 tcttcggtaa taaccgtttt atatttcagg tcggatttgc cctgatttaa aactacatat
     6601 ttctcataat aaacaatctc ttccaaattc ttagtcggaa tatccaataa ataatataac
     6661 ttgctaggaa ttgatttgag aaaccagata tggacaaccg gtacagccag ggtaatatgt
     6721 cccattcttt cgcgacgaac ttttttgcga gcgacttcta cgccgcaacg atcgcaaata
     6781 atgcctttat accggatacg tttatatttt ccgcaatggc attcccagtc ttttaccggg
     6841 ccaaaaatct tttcacaaaa cagcccatct ttctccggtt tataagaacg ataattaatc
     6901 gtctccggct tcagaacttc accgtgagac ctatttaaaa ttgtttcagg tgaagaaaga
     6961 tggatcgtaa tactttcaaa ttcgctacga aatgtagatg gttttgtata attcatactg
     7021 ataattatac ctccgtaatg ggattaatta atatccattt caatgcacaa gccttgtaat
     7081 tctttcaata aaacattaaa agattccggc acaccgaaat cggagaaatt ttcgcctttg
     7141 acaatggcat tatagacttt tgaacgaccg tcaacgtcat cggatttgac tgtcaagatt
     7201 tcctgcaaag tgtaagccgc accataggct tccagcgccc agacttccat ttcaccaaat
     7261 ctctgaccgc caaattgagc tttgccaccc agcggttgct gagtaactaa tgaatacgga
     7321 ccggtggaac gtgcatgcat tttgtcttcg actaagtgtg ataatttcat catatagatg
     7381 tagccgaccg tcaccggatt atcaaactgc tcaccggttt gaccatctat aaggctaacc
     7441 ttaccaattt ctggtaatcc ggctttgcgc aattcttcct gaacttcctg ctcgcttgcg
     7501 ccattgaaca cgggagtttc ataataaaca ccaagttcgc gccccgccca acctaacata
     7561 gtttcataca attgccccag gttcattcgg gaaggtacac ccatcggatt taggataata
     7621 tctaccggtg taccgtcttc gaggaaaggc atatcctctt taggaacgat tattgagacg
     7681 acacctttat taccatgacg gccagccatt ttatcaccaa cttgaacttt ccgtttgctg
     7741 gcaatataga ctttggctaa ttgtaagaca cccggctgaa ggtcatcgcc aactttaatc
     7801 ttataaatgg tttgttctaa ttcctcatcg attcgctgac taagaataaa ataactttta
     7861 acgattttct caactttttc gttgatttcc ggatcgtttg tccagggcgt ttccaggcta
     7921 agtagatcga aatctaactg ggctagaatt tcatcattaa attggatgcc tgacttgata
     7981 gcaactttgc cattggttag attcttaatc gtggcacttg tagcaccctt cagaatattt
     8041 ttcaatagaa gatcgcgtct tcctttaagg taagcatgct tttgaacact acgtttggtt
     8101 tcctcatcaa tctggcgctt ctcttccatc cgactgactt tacttttgcg agtaaaaagt
     8161 ttggtatcaa cggcaacgcc tctgatcccc ggatcagccc gtttggaggc atctttgaca
     8221 tcactggctt tctcgccgaa aatcgcccgc agtaattttt cttcaggagt cggatcggtc
     8281 tcacctttgg gagtaacttt accaattagt atgtcattag gttcgacttc cgcaccaacc
     8341 cgaatgattc cgttggcatc taaattctta gtagcttctt cactgacatt cggaatttca
     8401 cgagtcagct cttcgtttcc acgtttggta tctcgaactt ctagctcgaa ttccttgata
     8461 tgaatcgatg taaatacatc atcatagacc aagcgttcgt ttaaaacaat ggcatcttcg
     8521 aaattgtatc cgcgccaggg cataaaagcc accaacacat tacgaccaag cgccaactcg
     8581 ccatcattag tagcacaacc atcggcaatt gcatcaccgg cttttactcg atcaccttct
     8641 ttaatcaaag gcttctgatt tatgcaagta tcctgattag tacggaggaa cttagctaat
     8701 tcaatacgaa ctttaccttg attttcatct aaatacagtt cgttctcagt gtcggtgcga
     8761 attacgatgt gatcggcggt aacttcttct acaatgccgt ttaccggggc gagtacaact
     8821 gactgagaat cgcgggctac taccccttcc agaccggtac cgacaatggg acgctctggt
     8881 ttcaataatg gcactgattg tctctgcatg tttgaaccca ttaaagcccg gttggcatca
     8941 tcatgttcga gaaatggaat taatgcggcg gagaccgaga caatctgctc cggtgaaaca
     9001 tccatatatt taacttcgtc gggtgttaca attgggaatt cacccctaac ccttgccctc
     9061 acccgagaat taataaagtg cccttgttca tccagcggtt cattagcctg agcaattatc
     9121 tcgcggtctt catcatccgc tgacaagtac tcaactttat cagttaccgt agcgattccg
     9181 ttgacttttt caacacggcg atacggagtt tcgataaaac cgagatcatt gacataggca
     9241 taggtagcga gtgaagcaat caagccgata ttaggacctt ccggagtctc aatcggacat
     9301 aatcgtccat agtgagtata gtgcacatca cgtacctcaa atccagcacg ttcgcgggat
     9361 aagccacccg gtcctaacga tgaaactcgg cgcttatgag tgacttcagc cagaggattg
     9421 gtctgatcca taaattggga caattgactg gtcccgaaaa acgaatttat taccgaagtt
     9481 ataatgcgtg aattaattaa atcttgaggg gtgagatttt ccgattcgcg tagattcatt
     9541 ctctctctga ttgtgcgcgc cattctggta aatgccagat taaactggtt ggtgagctgt
     9601 tcaccaacgt ttcgcacccg ccgattcccg aggtggtcaa tgtcatctgg accgagattg
     9661 ccctttttca tatcaacgat acgcttcata atctcgacga tatcatcgcg tgtaagaaca
     9721 cattcttcca gcggcacgtt cagattaaat tttttattaa tccgataacg tccaacctgt
     9781 cccaaatcat acttcttggg actaaaaaat aaacgctcaa caaacttttt agcaatatcg
     9841 agattcggtg gctcgccagt tcttagactg cgatagagaa aatagagcgc agattcctca
     9901 tcattggttt gagtatcttt gcggaaggta tttaaaaaaa tattgcgctc gatttcatca
     9961 tcggtcgtga atactaatag ggaagtaatt ccatgttgtt tgcatttttt gagaaaatct
    10021 ttatcaacga cgatctcttc tttgatttca gaaataactt cgccggtctc gggatcaatg
    10081 acgttttccg ccagcatttt tcccagaagc tctttggaat ctccatcaag agaaaagcgt
    10141 ttgccgattc cgaataagtt gacgatgtct tcattggtcg gatagccaaa ggcacgcaat
    10201 aaaatggtcg ctggaaattt acggcggcga tctatgatag cactcagaat atcacgagta
    10261 tcaattacaa aatcaatcca tgaaccccgg aagggaataa ctcgtgccga ataaattttc
    10321 atgccattgg tatgttcggt ttcatcaaag aaaacacctg gtgaacgttg caattgactg
    10381 acaatagcgc gttcggcacc attaatgata aaggtacctt tttctgtcat aaagggaacg
    10441 tttccaaaga aaatatcctg ctcgatacca gcctgatact cgcctttgga aatgccttct
    10501 tcactgatat gaagcaccag acgaactttt aggggtacag tatagctgac acctcgttcg
    10561 agacactctt tgatagtgta acgcggttca ccaattgtgt aatatttgta ctcaagaaga
    10621 taattgccgt gggtatcttc gactggaaaa acgtttctga acgctgattc cagtccaata
    10681 tttttgcgtt ccagcggcat cgtatctttt tgtaaaaatt catcgaaaga ttttaactgc
    10741 acttccagca ggttaggcat cttttgcaca attttaactt tggcaaaaga tttcctttct
    10801 ttctctaaag ggagagtatt actcaacggt atccctcctg attaatttga ttttccatac
    10861 aatacaatga aaattcctta ttggcaaaac aagaattctc ataaatgctg aagattgaaa
    10921 ctgagtatag tttacttgac ttcgactttg gctccggctt cttcgagttg ttttttaagt
    10981 tcagccgctt cctctttgga aatgccctct ttgaccgtac tgggagcgcc atcaaccaaa
    11041 tctttggctt ctttcaatcc caaattggta gccgcccgca cgactttaat tacgttaatc
    11101 ttattagggc caacctcttt aagaataaca ttgaattcag tttgttcttc aaccgcttcg
    11161 gcttcagccg ttgccccagg ctgagctacc gtagccgcaa ctgcgacggg cgcggcggct
    11221 ttcacgtcga attttttctc gatgtcctta atgagttcag aaatttccag catatttgct
    11281 ttttccagat actccagaac gtctgctcta gttatgttcg ccatttctaa aaaacctcct
    11341 tttagtgcga tgcttatgat tgtttttttt gctcatttaa tgaatttaga gtgccgacta
    11401 actgatttaa cgtagcacgt agaaccatag tcagctggct catcggcgca tttagagtag
    11461 cgagtagaga agccagcagt tcccggcggg aaggtaaatt aatgactttc tcaacttgat
    11521 cggctccaaa tagcactcca tcatagatac aaccggtaat aaccagctta tccagtttat
    11581 ttttcttaat aaaagtgttg atcactttcc cgggtatagc gggatcagta taggaaattg
    11641 ctaacgcagt ttggccatct aataatggct cgatcgaatc taatcctgaa tttttagcgg
    11701 ccattttggt caaagtcttt ttaactactt tatattcgac atcagccgca tataattcct
    11761 tacgtaagtc ggtgatttct ttgacattta gacctttata attggtgaaa tagataccac
    11821 gggctttttg aaatttctca ctgatcgttt ccagactctg aatatttttc tgatttggca
    11881 tttttgctcc tgagattacg ctataattga ggatttatct acccgtactc cggggcccat
    11941 cgtcgtggtt agagtcactt tttgaatgta tgctccttta acagaaggtg ggcgtaaacg
    12001 cataagtgtt gagattaaag tctgtacatt ctcttcgaga tgctcgattg gaaatgactt
    12061 tttaccaacg atagtatgaa cgattccggt cttatctact cggatttcta ctcgaccggc
    12121 tttgatttct tttacagccg cggcaacatc attggttaca gtaccacttt tgggattcgg
    12181 catcaaacct tttggaccga gtatttttcc taatttacca acctgtccca ttacatccgg
    12241 agtggcaatg ataacatcta cgtcagtcca gccgttttga attttttcga gatattcttc
    12301 cagaccagca taatctgctc cggcttggag ggcttcatct actttactct tggtcaatac
    12361 taaaactcga actttgcggc cggtgccgtg cggataggtt actgtgcccc tgatattttg
    12421 atcggcatga cgcggatcaa ctcccaaatt aacatgggct tcgacggttt catcgaattt
    12481 ggctccgttg caatttttta gtaactcgag tgcttcccgc agagggtatt ccttcttccg
    12541 gtcaactttt ttactgttct gttcaactcg ctttgaaatg ttcataacgt tgaatcctct
    12601 tactcattta ttgaaatgcc catactccgg gcagtacctt taatcatttc cgcggctttt
    12661 tcaaccgtgt gggcatttaa atcaggcatt tttattttag caatttccat gacttggtcc
    12721 attgttaccg atccgatttt acttttatta ggttcacctg agcctttttc tactttggcg
    12781 gctttcaata gcaaaacgga tgccggcggt gtcttggtta taaaagtaaa tgaacgatct
    12841 gagtaaaccg taataactac gggaatgata taaccagctt gatcctgagt gcgagcatta
    12901 aacgccttac agaattcaac gatattgaca ctatgctgac ccaatgccgg gcctactgga
    12961 ggtgacggat tggctttgcc agcttcgatt tgcaatttaa tggtggctaa gacttttttc
    13021 gccatattac acctatttct ccaattctac ttgtaaaaag tccaactcga ctggtgttgg
    13081 acgcccaaaa atacttactg aaacttttaa tttacgtttg tcttcattga cttcctgaac
    13141 tactccgcgg aaatctataa aagggccatc aatgacttta accgggtcac ccattttaaa
    13201 cggaaccgct aacacctccc ggccgtcttt cttctcaact tcgcccatca ctcgtttaac
    13261 ttcatcttct ttcaaaggct gaggtttacc ggcggctccc acaaagttca ttactccggg
    13321 aatattctca atcagatatt gcgactcttt agtaatatcc atattgacca aaatataacc
    13381 aggaaagaag actttgttgc gaacgcgttt cttaccctcg cgcatttcca taatattttc
    13441 tgaaggcacc agtacatctt tgataatatc tttcatccca gcatgctcgg cttcaatcag
    13501 aatagcttct ctgacttttt tttccttacc ggaatatgtt cttaatacat accattccat
    13561 atcggacctg ctaaaaaatt acattcataa tttttgatag aatcagatca acaacgaaaa
    13621 gaaataccga taaaatcaat gagaaaatca gaacaacggc ggttgaactt ttaagctccg
    13681 accaggtcgg ccaggagact tttttcatct cgaagacaac cccttcaaaa aattcgcgaa
    13741 tgcgtttgat catgtctttg tcctccgttt gggcgatcgg taaggtctat tagcaatatt
    13801 gcaggtgagg caggaattga acccgcaacc cccggttttg gagaccggtg ctctgccagt
    13861 tgagctactc acctttagtt gcatattaac gggtctcttt atgaactgta tgttttttgc
    13921 accaacgaca gtatttttta tattcgaccc ggttaggctg tagcttctta ttcttatcag
    13981 aagaataatt tcgacgttta caaaccgtgc attccagagt aattaattca cgcatattat
    14041 tccctgaaat ttattcaatg atttcggtaa cgacgccagc accaactgtc cgtccacctt
    14101 ctcgg
//
LOCUS       DAOOMU010000057        14075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4678_length_14075_cov_19.505635, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000057 DAOOMU010000000
VERSION     DAOOMU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 14075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14075
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4678_length_14075_cov_19.505635"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1191)
                     /locus_tag="PLI55_06590"
     CDS             complement(157..1191)
                     /locus_tag="PLI55_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPB00538.1"
                     /translation="MPKSIATYILPLAIVIISALIGLLLDRLVFSKWRKNVQKIRPPV
                     LRVVGNSMRGILIIVMGLVGLYFALSYFGIEEEVLNNLRKLLIVLIILTFTILLARIA
                     VGFIENLGRRKKMPSLTLFINLTKIVIYVIGFLIILQTLGVSITPLLTALGVGGLAVA
                     LALQDTLSNLFAGIFIIGTRLVQPDDYIMLSSGVEGFVKDINWRNTIIRTMANNENVI
                     PNSELSKTIITNYSRPDKKMSLIVPVLVGYNNDLNKVEEVLIQTAREVLNEVEGGVKD
                     SEPIVRFNRFNESSVSLNLIVKVRKYEVQFPVQHELIKRIHRRFKEENIEIPYPVRTV
                     YLKPGSENEI"
     gene            1494..1967
                     /locus_tag="PLI55_06595"
     CDS             1494..1967
                     /locus_tag="PLI55_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HPB00539.1"
                     /translation="MNTKRDRHRAIRQIIASRQISNQNELKDVLEKQGFVTTQATLSR
                     DLMTLKIIKVPDAERGYIYALSENFQSSKPPIEGSSPLQTCRSIAFSGNLAVLKCLPS
                     FAPSVALLLDAYEMNEIIGTIAGDDTVLIILKEGLSHERFRSALVERCPELRNRI"
     gene            2044..3294
                     /locus_tag="PLI55_06600"
     CDS             2044..3294
                     /locus_tag="PLI55_06600"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPB00540.1"
                     /translation="MSKKVILAYSGGLDTSVILKWLVNKGYDVICYVANVGQREDFAA
                     VEQKAFKTGASKVYIEDLRREFVNDYIFAALRGNAIYEGRYLLGTSLARPLIAQRQVE
                     IAQMEKTHYYAHGATGKGNDQVRFEFAYYALDTEAIVISPWKDPEFLAEFKGRSDLIR
                     YAEKWNIPVKASLTKSYSEDENLMHISHEAGVLEDPSFRPPENVFSWTVSPQDAPDSE
                     TILEIEFKDGYPIKVTNLNNGVVKEDSLELFYYLNEIGSQNGVGRVDMVENRFVGIKS
                     RGVYETPGATILWTAHRDLEGIAMDKEVMHLRDMLSPKFAELIYNGFWFSPEMDFLMN
                     AFEKSQEKIDGSVTVSIYKGNVTPIGRRSPISLYDQALSSMDIAGGFNANDSLGFIRV
                     NALRLKAHKIVLKKKKPYNWRIKD"
     gene            3298..4497
                     /gene="argH"
                     /locus_tag="PLI55_06605"
     CDS             3298..4497
                     /gene="argH"
                     /locus_tag="PLI55_06605"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPB00541.1"
                     /translation="MKLWQKKYDLNAAVEKFTVGNDYLLDQELVPFDCQASRAHARML
                     NKIGILSDSETADLLSGIDQILDLHAKGKFKIRPEDEDCHTAIENFLTQKIGIAGEKI
                     HTGRSRNDQVLTALRLYCKTQLNEISSAVQTTIATITEFIQKYGEVKMPGFTHTRKAM
                     PSSVAMWAGALRDALADDLDLLSCATKLIDQNPLGTGAGFGVPLALDRQMTTDELHFA
                     RIQENPIYAQNSRGKFEGFALSVMSQIMYDLNKVASDLILFTMPEFNYFTLPDEFLTG
                     SSIMPHKKNPDVLELLRAKYHEVFSYEMQVRNTGVNLISGYHRDLQLTKEAIIRAFKT
                     TLTSVQITTVLFENLSVNRKNCRKAMTAELFATDKVYQLVKSGVPFREAYRQISQEYE
                     DKNEKQC"
     gene            4481..5515
                     /gene="argC"
                     /locus_tag="PLI55_06610"
     CDS             4481..5515
                     /gene="argC"
                     /locus_tag="PLI55_06610"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPB00542.1"
                     /translation="MKNNAKIRVSVVGATGYAGFELVKILLRHPYVTINRLAVIAEEV
                     GLCYSDIFPALKGLCDLTCTAIDVQEIKRESDVIFFCLPHTVSMTYVPDFVGNEKLLI
                     DLSADYRLNSVELYQKAYGVAHTDSEHLPDFVYGLPEFNRAKIKSAKTIANPGCFPTG
                     IELALKPVLQAGLIETTAIFVDSKTGISGAGRTPKPAYHFPECNENLTAYKVASHQHE
                     PEIEQELTKIAGSPVDVIFVPHLVPLTRGIFSTIYTKLRQTISQTELLTLYQNDYQAE
                     PFVRVLSNNQIPQLKNVANSNFCDIGLKVIGDHLILLSAIDNLIKGASGQAVQNMNIM
                     LGYPETTGLL"
     gene            5653..6861
                     /gene="argJ"
                     /locus_tag="PLI55_06615"
     CDS             5653..6861
                     /gene="argJ"
                     /locus_tag="PLI55_06615"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPB00543.1"
                     /translation="MNFYNNPKITDPKGFTGAAMHAGFKRKRNDLCIIKSTVPAKCSA
                     KFTTNAVKAAPLIVAMENLPKSGNRLQALIINSGNANAGTGQQGLNDVHETLNLLATQ
                     LDIPEELILASSTGVIGHCLDMEKMRNGITHLVPHLSIEGGAACAEAILTTDTVEKSF
                     GVKFRVNGAEIKLCGITKGSGMIQPNMATTLAFVTTDADIDHNFLEKTFTTTVAKTYN
                     MITVDGHTSTNDTALIMANGLAGNQIITAGSPAGEIFENALLMLMTEMAKSIVRDGEG
                     ATKFVTVTVNGADSFEDAKKMAFGIANSMLVKAALFGCDPNWGRILSAAGMVFAHFDP
                     AVADLRINGIYLYRQGEPLNLNKTEIQRMMTPVELEIVLDCNLGKDSAVVYTTDLSYD
                     YIKINAQYHT"
     gene            6870..7763
                     /gene="argB"
                     /locus_tag="PLI55_06620"
     CDS             6870..7763
                     /gene="argB"
                     /locus_tag="PLI55_06620"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPB00544.1"
                     /translation="MQKIIDKAAVLIEALPFIKAFHGKKIVIKFGGSIMDSENAMKSV
                     LQDIVFMKYVGWLPVVIHGGGKAISEAMNNRGVKPQFVNGLRVTDRKTMEIVENVLAH
                     KINAQIVNLLNMMDGEAVGVSGLDNGLIRVDKMFTENNGQSVDLGFVGQVRYINANFL
                     NRLIESGKIPVIAPLGLGDDGESYNINADLVAGEIAASIGARKLVYLTDTPGILRTPG
                     DEKSIISTINRQEAEKLIAAGIIEGGMIPKVNSCLDALRFGVNKGHIIDGRIKHSLLL
                     EIFTKEGIGTEILKDGSERIR"
     gene            7741..8904
                     /locus_tag="PLI55_06625"
     CDS             7741..8904
                     /locus_tag="PLI55_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPB00545.1"
                     /translation="MDRKEFDNYVLPTYKRFPITLVKGRGMQVWDDEGNEYLDFFPGW
                     GCGNIGHCHPKVVAALHEQLDKIMHVPNVYYNEKAGELAKLIIQDSFDGQVFFGNSGA
                     EANEGALKLVRKRNPNKKTIITLNNSFHGRTLATVTATGQSEYQKGFDPLLGGFVYCD
                     INDYAMLASLMNENVAAIMLEVVQGEGGITPVTPKFIQTARELCDRFDAMLIIDEVQT
                     GIGRTGKMFAYQYFDVQPDVMTLAKSLGGGMPIGAFAVARQYCNILQPGSHGSTFGGN
                     PLACAAGVAVFQAIHEENLLENAVEMGTYFRQKLEELKSQAPVIREIRGLGLMLGVEL
                     NKPGAEIVDKCRAQNLLINCTHQTVLRLMPAINVTKEQIDKAVAIIGKALMES"
     gene            9082..11550
                     /gene="thrA"
                     /locus_tag="PLI55_06630"
     CDS             9082..11550
                     /gene="thrA"
                     /locus_tag="PLI55_06630"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HPB00546.1"
                     /translation="MKVLKFGGSSIGKPENLPTIFNIIAREVQSEKAVITVFSAFNNV
                     TDQLKEISQLAASKDKTYQNILDDLARKHFNFIEKLIPQDKQKTLNAEIKNLLAELKE
                     IIHGVFLLKELSLQSLDLILSFGERLACTIIAAYGQSIGYPTKYVDARQFLVTDDHFG
                     AARILPHISNPKIQAFFKNFTGIPVVTGFIGATADGITTTLGRGGSDLTASQIGAALN
                     AEEIQIWKDVNGFMSADPNKVKEAFSLAELSYEEAVELSHFGAKVLFPPTIQPVIDKN
                     IPIRIMNTFNPDFPGTVIRKNPSANGGYVKGVTSISDISLLTVQGSGMVGVSGISSRL
                     FSALAQVNVNVILISQASSEHSICVAILRESVNIAKKAIEEEFALEIKLRLVDPVVVE
                     NDLVIIAVVGERMRRTTGLAGRLFSALGEKSINVIAIAQGSSELNISIVIDQKDEKQA
                     LNAIHNAFFAKKIKMNLFLCGVGLIGSTLIEQIKTNYSNLLENYGLDIKIVGLANSRK
                     MLIEPDGIDLNNWRNSLNECQVKNDMRVLLAEIKKLNLDNNVFVDCTASADIVNYYEK
                     ILAGRTSIVTANKIGNTGPYAQYVKFHELARSKHVHFLYETNAGAALPIISTLRDMIN
                     SGDQIIRIEAILSGTISYILNNFKPGKKFSAIVREAKARGFTEPDPRDDLSGIDFARK
                     VLLLVREIGIPAELSDIQIEPLLPENCLKAPTVEAFFTELEKSNDYFDKLLQQASAEG
                     KVLRYIAIYENEKAELKLVQVEANHPFYFLSGSDNIVAFTTKRYAENPLVIKGAGAGA
                     EVTAAGVMADIFKIANAVMKSKSF"
     gene            11550..12488
                     /locus_tag="PLI55_06635"
     CDS             11550..12488
                     /locus_tag="PLI55_06635"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HPB00547.1"
                     /translation="MKSVTVFAPATVANVACGFDIFGFAVNEPGDVAKVTLRENPGVE
                     ITRITGDNGKLPKAAEKNTAGFAALRYLETIQSQQGVSIELHKKMPLSSGLGSSAASA
                     VAVLFAINTLFDNRLTKSELLSIALQSEKLACGAAHADNVAPALFGGFLLIRENNPPD
                     IVELPVPENLYCTIIHPEIEINTADARNILAPNVPLKDAVKQWANTAGLVAGLYRKDL
                     TLISRSMNDYIIEPQRARLIPHFYEMKKAALDRGALGCSISGSGPSLFALSNSQEIAQ
                     KIAGSMSAVLANYKICNQIYISTINTEGPKIIAQEG"
     gene            12492..13793
                     /gene="thrC"
                     /locus_tag="PLI55_06640"
     CDS             12492..13793
                     /gene="thrC"
                     /locus_tag="PLI55_06640"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPB00548.1"
                     /translation="MLYYSTTDRSHKVSLKEAVLKGMPPDGGLYLPESLPVMPDKFFQ
                     DLPEMTFQSVSYEIAKALLGGDVPSDVLQKIIVEALNFETPLVAISEKIKTLELFHGP
                     TLAFKDVGARFMSRLMAYFIRNDCRELTILVATSGDTGSAVANGFFQVPGINVIVLYP
                     AGKISLIQEKQIATLGENITALKVEGTFDDCQRLVKTALVDPEVRSVLTVSSANSINI
                     ARLIPQTFYYFNAIRQIPNFHSRKIAICVPSGNFGNLTAGLIAKKMGLPVIRFIAATN
                     INHVVPDYLATGKYEPRASIQTISNAMDVGNPSNFARMLALYDYSVEKMRRDLAGCYF
                     NDDETRAAIAEVYRNYGYILDPHGAVGYLGIQNYLAKHPEIDTGIFLETAHPAKFCDI
                     VEPVIGTKVPIPDRLKKFLERPILSRAISSDYDEFKDLLRA"
BASE COUNT         3998 a         3293 c         2977 g         3807 t
ORIGIN      
        1 attaaaagtc aagagttgta ttacaaattt tgaaatgttt aacatctttt gtcgcggtca
       61 cgacctaatc tctattgttc ttcttccaaa agtcaaatat aatggattca acttaaagga
      121 ttttcggaat ttaaattgtc ccctaaacta ctaaggtcag atttcgtttt cacttcccgg
      181 ttttaaataa acggtacgca ccggataggg tatctcgata ttttcctctt taaagcgccg
      241 atgaatccgt ttgattagtt catgctggac gggaaactga acctcgtatt tccgcacttt
      301 gacaatcaaa ttgagactaa cgctactttc attaaaacga ttaaagcgca cgataggttc
      361 gctatctttt acacctccct caacttcgtt taaaacttct ctggcagtct ggattaaaac
      421 ttcttcaact ttatttagat cattgttata gccgaccaat accggaacta tgagagacat
      481 ttttttatct ggacggctat aattagtgat aattgtcttg gacagctctg aattaggaat
      541 aacattctcg ttgttcgcca tagttcggat aatcgtattg cgccagttga tatctttaac
      601 aaatccttct accccgctcg acaacattat ataatcatcc ggctgaacca acctcgtacc
      661 gataataaaa ataccggcaa aaagattaga aagcgtgtcc tgtagtgcca atgccaccgc
      721 caagccgccg actcctaaag ccgttagcaa tggcgtaatt gaaacaccta gtgtttgtag
      781 aattatcaaa aatcctatta cgtaaatgac aatttttgtc aggttaataa agagagtcag
      841 agaaggcatt tttttacggc gaccaagatt ttcgataaaa ccaaccgcaa tccgtgccag
      901 caaaatggta aacgtcaaaa taatcagcac tattagtaac ttgcgcaagt tatttagaac
      961 ttcttcctca attccaaaat aacttaaagc aaaatacaag cccaccaatc ccatcacgat
     1021 tatcaaaatg ccacgcattg aattaccaac aacccttaac accgggggtc tgattttttg
     1081 tacgtttttc ctccatttac taaacactaa cctatccagc agtaatccga ttaaggcact
     1141 aattattact attgctaatg gtaaaatgta agttgcaatc gacttgggca ttttctatcc
     1201 ttttaatttt ttaataaaaa cgtttgattc agctcgcggc actctcttat ctcattccca
     1261 attacctcta ataataacga ttccgcatct ttatttcaag aataatatcc taaaaaacgc
     1321 tttctctact ttatatcata gtaccaaata aaattacaca caattaattg tctaaaatgc
     1381 acttaaaatc ttgatagttg acttttattg cttcaatcat aaaaaattat ttgcatatat
     1441 gtacggcgga ttgtatattt attcgctttt taagtatatt tatacgtttg aagatgaata
     1501 ctaagagaga ccgacacaga gctatccggc aaattattgc ctcccggcaa atttctaatc
     1561 agaacgaact aaaagacgtt ctcgaaaaac agggttttgt tacgactcag gcaactcttt
     1621 cgcgtgattt aatgactttg aaaatcatca aggtccctga cgccgaaaga gggtatattt
     1681 atgcgctttc cgaaaacttt caaagttcaa agccgccaat cgagggcagt tcacccttgc
     1741 aaacctgccg atcgatcgcc ttttccggca atctcgccgt gctcaaatgc ctgccttctt
     1801 ttgctcccag tgtcgctctg cttctcgatg cctatgaaat gaatgaaata atcggcacaa
     1861 ttgccggtga tgataccgtt ctaattattc taaaagaagg attgtctcac gagcgctttc
     1921 gatcggcgct cgtggaacgc tgcccggaat tacgtaatcg aatttagacc gggattttca
     1981 taaagttaac cttaacaaat acatctatcc ggtagaatca actattttga aaggaattaa
     2041 tcaatgtcaa aaaaagtaat attagcatac agtggaggtc tcgacacctc tgtgatcctc
     2101 aaatggctgg tcaataaagg ctacgacgta atttgctacg tcgccaatgt cggtcagcgg
     2161 gaagatttcg ccgccgtcga gcaaaaagcc ttcaaaaccg gcgcttcgaa agtctatatc
     2221 gaagacctgc gtcgtgaatt cgtgaatgac tatattttcg cggctttgcg tggcaatgcc
     2281 atttacgaag ggcgctattt attgggcaca tctctggcgc gtcctttaat tgcccagcgg
     2341 caagtcgaga tcgcccagat ggaaaaaact cattactacg ctcacggtgc gaccggcaaa
     2401 ggcaatgacc aagttcgttt cgaattcgct tactatgccc ttgacacgga agcgattgtt
     2461 atttctccct ggaaagatcc ggaatttctc gccgaattca aaggtcgcag tgatttgatc
     2521 agatatgcgg aaaagtggaa tatacccgtc aaagcttcct taacaaaatc ttacagtgaa
     2581 gatgaaaacc tgatgcacat tagccacgaa gccggtgtcc ttgaagatcc ctctttccga
     2641 ccgccggaaa atgtcttcag ctggactgtt tcgccgcaag acgctccgga tagcgagacg
     2701 attctggaaa tagaattcaa ggacggttac ccgattaaag ttacaaatct aaacaacggc
     2761 gtcgtcaaag aagactcctt ggaacttttt tattacctta atgaaattgg gagtcagaac
     2821 ggcgtcgggc gagttgatat ggtcgagaac cgcttcgtcg gcattaagtc gcgcggcgtc
     2881 tatgaaactc ccggcgcaac gattttatgg acggcgcacc gcgacctgga aggtattgct
     2941 atggataagg aagtaatgca cctgcgggat atgctttcgc ctaaatttgc cgaattaatc
     3001 tataacggct tctggttttc acctgaaatg gattttctaa tgaatgcttt cgagaaaagt
     3061 caggaaaaga ttgacggctc agttacagtt tccatataca aaggcaatgt tactcccatc
     3121 ggacgtcgtt cgccgatttc gctctacgat caggctctat cgagtatgga catcgcaggt
     3181 ggtttcaatg ctaatgacag tctgggcttt attcgcgtca atgctcttcg tctgaaagcc
     3241 cataaaatag tcctcaaaaa gaaaaaacct tacaactggc gcatcaagga ctaacctatg
     3301 aaactctggc aaaaaaaata tgatctcaac gccgccgtcg aaaaattcac cgtcggcaac
     3361 gactacctgc tcgatcaaga actcgttccg ttcgactgtc aggcttcccg agctcacgct
     3421 cgaatgctga ataaaatcgg cattctctcg gactcggaaa cggccgattt gctcagtgga
     3481 attgatcaaa tcctcgattt acacgccaaa ggcaaattta aaattcgccc cgaagacgaa
     3541 gattgccaca cggcgatcga aaatttcctg acgcagaaaa tcggcatagc cggtgaaaaa
     3601 atccataccg gtcgcagtcg taatgaccag gttctaaccg ccttacgtct ttactgcaaa
     3661 actcaattaa acgagatttc ctctgccgtt caaactacta tcgcgactat caccgagttt
     3721 atccaaaaat atggcgaggt taagatgccc ggttttacgc atacgcgcaa agcgatgcct
     3781 tccagcgtag caatgtgggc tggtgccctt cgagatgccc ttgccgatga ccttgatttg
     3841 ctatcctgtg ctacgaaatt gattgatcaa aatccgctcg gcaccggcgc tggatttggt
     3901 gttccgctag ctctcgaccg ccaaatgaca actgacgagt tacatttcgc ccgtattcaa
     3961 gaaaatccga tttacgccca gaacagtcgc ggcaaattcg agggttttgc tctcagtgta
     4021 atgtcgcaaa taatgtatga tttaaacaaa gttgcctccg atctcatttt atttaccatg
     4081 ccggagttta attatttcac tctgccggat gaatttctaa ccggctcatc cattatgccg
     4141 cataagaaaa atccggacgt gcttgaatta ttgcgggcta aatatcacga ggttttctct
     4201 tatgaaatgc aggttcgcaa taccggcgtc aacctgattt ccggctatca tcgcgacctg
     4261 caattgacca aagaagccat catccgagca tttaaaacga ccctaacgtc ggttcaaatc
     4321 actactgttt tatttgaaaa tttatcggtt aaccgtaaaa actgccggaa agctatgacc
     4381 gccgaattat ttgctaccga taaggtttat caattagtaa aaagcggcgt gcctttccgc
     4441 gaagcttacc gtcaaatcag tcaggaatat gaggataaaa atgaaaaaca atgctaaaat
     4501 tcgtgtatct gtagtcggtg ctaccggcta cgccggtttc gaactggtaa aaattctact
     4561 gcgtcacccg tacgtaacaa tcaaccgttt ggcagtaatt gcggaagagg ttggcttatg
     4621 ttattcggac atttttccgg cactgaaagg actttgcgat ctgacctgca ctgcgattga
     4681 cgttcaggaa atcaaacgcg agagtgacgt aattttcttt tgtctgccgc atacggttag
     4741 tatgacttac gtcccggatt tcgtgggaaa tgaaaaatta ttaatcgatc ttagtgccga
     4801 ctatcgcctt aattcagtcg aactttacca gaaagcttat ggcgttgccc acaccgatag
     4861 cgagcatttg ccggattttg tctatggtct gccggaattt aaccgcgcta aaattaaatc
     4921 agcaaaaacc atcgccaatc ccggatgttt tcctaccgga atagaattgg ctttgaagcc
     4981 ggttttgcaa gccggattaa tcgaaaccac ggcgattttc gttgatagca aaaccgggat
     5041 ttccggcgcg ggacgtactc ccaaaccggc ttaccatttc ccggaatgta atgagaacct
     5101 gaccgcctat aaagtcgcct cgcatcaaca tgagccggaa attgagcagg aattgaccaa
     5161 aatagccggc tcgccggttg atgtaatttt cgtgccgcat cttgttcctt tgacgcgtgg
     5221 tatttttagc acgatttaca ctaaattacg gcagacaatt tctcaaaccg aattattaac
     5281 cttatatcaa aatgactatc aagcggaacc tttcgtgcgc gttctatcaa ataaccaaat
     5341 tccccaatta aaaaacgttg ctaatagcaa tttttgcgat atcggtttaa aagttatcgg
     5401 cgatcacttg attctgctaa gcgcgattga caatttaatc aaaggtgcct ccgggcaggc
     5461 cgtccagaat atgaacatca tgctcggtta tcctgaaaca actggattat tatagggatt
     5521 agaaatgagt ataattcagc cgatttttaa tagccttgat ttttctttag ttcatttctt
     5581 tggattaaaa caaagaaacg aacaggtttt tattaaagaa attaatttta atttttaatg
     5641 aatggattcg ctatgaattt ttacaataat cctaaaatca ccgacccaaa aggttttacc
     5701 ggtgcggcga tgcatgccgg cttcaagcgc aaacgcaatg acctgtgtat aatcaaaagc
     5761 accgtgccgg ctaaatgttc agccaaattc accaccaatg ccgtcaaagc cgcgccgctt
     5821 atcgtcgcta tggaaaattt accgaaatcc ggcaatcgcc ttcaggcttt gatcatcaat
     5881 tccggtaatg ctaatgccgg taccggtcag cagggtttga acgacgtgca cgagactcta
     5941 aatttgcttg ccacccagct tgatattccg gaggaactaa ttctggcttc cagcactggt
     6001 gtaatcggcc attgcctcga tatggaaaaa atgcgaaatg gcattaccca cctcgtaccg
     6061 catcttagta tagaaggtgg tgccgcctgc gccgaagcga tattgacgac cgataccgtc
     6121 gaaaaatcgt tcggtgtaaa attcagagtc aatggcgcgg aaatcaagct ttgcggcatt
     6181 accaaaggtt ccggtatgat tcagcctaat atggcgacca cactggcttt tgtcactact
     6241 gatgccgata ttgaccacaa ttttctggaa aaaactttca ccacgactgt cgcaaaaact
     6301 tacaatatga ttaccgttga tggccacacc agcaccaatg atactgccct gattatggcg
     6361 aacggtttag cgggtaatca aataattacc gccggttcgc cagctggtga aattttcgaa
     6421 aatgctctcc taatgctgat gaccgaaatg gcaaaatcca ttgtccgaga cggcgaaggc
     6481 gccaccaagt tcgtgaccgt tactgtcaat ggcgccgata gttttgaaga tgctaaaaaa
     6541 atggcgttcg gtattgctaa ttccatgctg gtcaaagccg cgctttttgg atgcgacccg
     6601 aattggggca gaattctctc cgcggcgggg atggtatttg cccattttga tccggctgtc
     6661 gctgatttac gtatcaatgg gatttacctc taccggcagg gtgaaccgct taatttgaat
     6721 aaaaccgaaa ttcaacgtat gatgacaccg gtcgaactgg agattgtact cgactgcaac
     6781 ctcggaaaag atagcgccgt agtttacact accgaccttt cctatgacta tatcaaaatt
     6841 aacgctcaat atcatactta ggagctgtta tgcagaaaat tattgacaaa gccgccgttt
     6901 tgatagaagc tttacctttc atcaaagcct ttcacggcaa gaaaatcgtg atcaaattcg
     6961 gcggcagtat aatggacagt gaaaatgcta tgaaatctgt tttgcaggac attgtcttta
     7021 tgaaatacgt cggctggttg ccggtggtta tccacggcgg cggcaaagcc attagcgagg
     7081 caatgaataa ccgtggagtt aaaccgcaat tcgttaatgg actgcgcgta accgaccgca
     7141 aaactatgga aatcgttgaa aatgttctcg cccacaaaat caatgcccaa attgttaact
     7201 tgctgaatat gatggacggc gaggcggttg gagtttccgg tctcgataac ggattaatac
     7261 gcgttgataa aatgttcacc gaaaacaatg ggcaatcggt agacctcggt ttcgttggtc
     7321 aggttcgcta cattaatgct aattttctta atcggcttat agaaagcgga aaaattcctg
     7381 taattgcacc gctcggtttg ggtgacgatg gcgagagcta caatatcaat gccgaccttg
     7441 ttgccggcga aatcgcggct tcaattggcg ctcgcaagct agtttattta actgataccc
     7501 ccggcatttt gcggacaccc ggcgacgaga aaagtatcat tagtaccatt aatcgccagg
     7561 aggctgaaaa acttatcgcc gcgggcatca tcgagggcgg tatgattccc aaagtcaact
     7621 cctgtctcga tgctttgagg tttggggtca acaaaggtca cattatagac gggcgcatta
     7681 agcactcgct cttactcgaa attttcacta aagaaggaat aggaacggag atattaaaag
     7741 atggatcgga aagaattcga taattacgtt ttacctactt ataagcgttt cccgattacc
     7801 ctggtaaaag gacgcggtat gcaggtctgg gatgatgaag gcaacgagta cctcgacttt
     7861 ttccccggtt ggggttgtgg caatatcggg cattgccatc ccaaagtcgt ggcggctctg
     7921 catgaacaat tggacaaaat catgcacgta ccgaatgtct attataacga gaaagccggc
     7981 gaacttgcca aactaatcat tcaggacagc tttgatggac aagttttctt cggcaacagc
     8041 ggcgcggaag ccaatgaagg agcactgaag ctggtgcgca aacgcaaccc caataagaaa
     8101 acgattatta cgctgaataa ctcctttcat ggacgcacgc tggctacagt taccgcaacc
     8161 ggtcagagcg aataccagaa aggctttgat cccctgctcg gcggttttgt ctattgcgat
     8221 atcaatgatt acgctatgct cgcaagctta atgaacgaaa acgtcgcggc tattatgctc
     8281 gaagtcgtcc agggcgaagg cggcattaca cctgtcacac caaaatttat tcagacagcg
     8341 cgcgaactgt gcgaccggtt tgacgctatg ctaatcatag atgaagttca gaccggcatc
     8401 gggcgcaccg gtaaaatgtt tgcctaccaa tatttcgatg tccaaccaga cgttatgact
     8461 ctcgccaaat cactcggcgg cggtatgccg atcggcgctt ttgccgtagc acgccaatat
     8521 tgcaatattt tacaacccgg cagtcacggc tcgactttcg gtggtaatcc actggcttgc
     8581 gccgctggtg tggcggtttt tcaagctatt cacgaagaaa atctgctcga gaatgccgtt
     8641 gaaatgggca cctatttccg gcaaaaattg gaagaactga aaagccaagc gccggtaatt
     8701 cgtgaaatcc gcgggctcgg acttatgctg ggcgtcgaat taaataagcc cggcgcggaa
     8761 atcgttgaca agtgccgcgc ccagaatcta ctgattaatt gcacacacca gaccgttctg
     8821 cgcctgatgc ccgccatcaa cgtcactaaa gagcagatcg ataaagctgt tgcgattatc
     8881 gggaaagcct tgatggaatc ataggcaaaa ccttccgaag gttgggttag gctatttgaa
     8941 gctatctgaa ggttttatca attagtttcg tgtttaatcc gtagttaaat ctatctctgt
     9001 atttggcttg gcaggttctt aaaaaaatcg tattttctct cgctataatt gaacaattga
     9061 caaccagata gaaagacagt tatgaaagtc ttaaaattcg gcggcagttc gattggtaag
     9121 ccagaaaatc tgccaactat tttcaatatc atcgccaggg aagtccagag cgagaaggcg
     9181 gtaattacag ttttttcagc cttcaataat gtcactgatc aattgaaaga aatcagccaa
     9241 ttagcggcaa gcaaagataa aacctatcaa aatattcttg atgaccttgc ccgaaaacat
     9301 tttaatttta tcgaaaaatt aataccccaa gataagcaga agacactcaa cgccgaaatc
     9361 aaaaacctcc tggcagaatt gaaagaaatt atccacggcg ttttcttatt gaaagaatta
     9421 tccttgcaat ctctcgacct gatcctgagt ttcggagaac gcttggcttg caccatcatc
     9481 gccgcctatg gtcaatccat cggctaccca acaaagtatg tggatgcgcg ccaatttctg
     9541 gtaaccgacg atcatttcgg agcggcacgt attcttccgc acatcagcaa tccgaaaatc
     9601 caagctttct ttaaaaattt taccggaatt ccagtagtca ccggtttcat cggcgctacc
     9661 gcagatggca ttactacaac tttaggccgc ggcggttcgg acttaactgc ctctcaaatc
     9721 ggtgcggctc ttaacgcgga ggagattcaa atctggaaag acgtcaatgg ctttatgtcg
     9781 gctgatccaa acaaagtcaa agaagctttt tcattagcgg aactgagcta tgaagaagct
     9841 gtggaactca gtcatttcgg cgccaaagta ctatttccac ccactattca gccggtaatt
     9901 gataagaata ttcctattcg tataatgaat actttcaatc cggattttcc cggaacggtc
     9961 attcgcaaaa atccctccgc caacggtggc tacgtcaaag gtgttacttc aatcagcgac
    10021 atttcattgt tgacagttca aggtagtggc atggttggcg ttagtggcat ctcttctcgt
    10081 cttttcagcg ctctggcaca agtcaacgtc aacgtgattt tgatttccca agcctcgtct
    10141 gaacattcca tctgtgtcgc cattttgcga gaatctgtca atatcgcaaa aaaagccatc
    10201 gaagaagaat tcgctcttga aattaaactc cgcctggttg acccggtcgt agtagaaaat
    10261 gatttagtca tcattgcggt tgttggagag cgaatgcgtc gcaccactgg tctggcggga
    10321 cgtcttttta gcgcattggg agaaaaatcc attaacgtca ttgccatcgc tcaaggttcc
    10381 tcggaactga atatttccat cgtaattgac caaaaagatg aaaaacaagc ccttaatgcc
    10441 attcataatg ctttcttcgc taaaaagatt aaaatgaatt tatttctctg cggggtgggt
    10501 ttaatcggat caactttaat cgaacagatt aaaaccaatt attccaatct actggaaaat
    10561 tatgggctgg atattaaaat cgtcggtctt gctaatagcc ggaaaatgct aatcgagccc
    10621 gacggaattg acctcaacaa ttggcgcaat tctctcaatg agtgtcaggt caaaaacgat
    10681 atgcgggttt tactggcaga aataaaaaaa ctcaatctcg acaacaatgt cttcgtagat
    10741 tgcaccgcca gcgccgatat cgtgaattat tacgaaaaaa ttttagccgg tcgcacctcc
    10801 atagtaactg ctaataaaat cggcaacact ggcccatacg ctcaatatgt aaaattccac
    10861 gagctggcga gaagtaaaca cgtccatttt ctctacgaaa caaatgccgg tgcggctctg
    10921 ccaattatca gcactctgcg cgatatgatc aattccggcg accaaatcat ccgaatcgaa
    10981 gcaattcttt ctggcacaat tagttatatt ctcaataatt tcaaaccggg gaaaaagttc
    11041 agcgccattg tgcgcgaagc caaagccaga ggttttaccg aacccgatcc acgcgacgat
    11101 cttagtggga tagatttcgc ccgcaaggtt ttactgctcg tccgcgaaat cggcattccc
    11161 gccgaactct ccgacattca aatcgaaccg cttttgccgg aaaattgtct caaagctccg
    11221 acggttgaag cgtttttcac cgaactcgaa aaatccaatg actattttga taaattgctc
    11281 cagcaagcct ctgcggaagg taaagtatta cgctatatcg caatttatga aaatgaaaaa
    11341 gctgaattga aattagttca ggtagaagcg aatcatccct tctacttctt gagcggtagt
    11401 gacaacatcg tcgctttcac tacgaaacgc tacgctgaaa atccgttggt tatcaaaggc
    11461 gcgggggctg gggcggaagt caccgccgcc ggtgtaatgg ctgacatttt taaaattgcc
    11521 aacgccgtta tgaaaagcaa gagtttttga tgaagtctgt cactgttttc gcaccggcta
    11581 ctgtggctaa tgttgcttgc ggtttcgata ttttcggttt tgctgttaat gaaccgggcg
    11641 acgttgctaa ggttactctc cgcgaaaatc ccggcgtcga aattacacgc ataaccggcg
    11701 acaacgggaa attgccaaaa gccgccgaaa aaaacaccgc cggattcgcc gccctccgtt
    11761 atctcgaaac cattcaatcc cagcagggtg tttccatcga attacataaa aaaatgccct
    11821 tgagtagtgg actgggatcg agcgccgcca gtgcggtggc ggtactcttt gccattaaca
    11881 ctcttttcga taatcggctg acgaaatccg aactgctcag tatcgctctg caatcggaaa
    11941 aattggcttg cggcgccgcc catgccgaca acgtcgcacc ggctctcttt ggtggctttc
    12001 ttttaattcg ggaaaacaat ccgcctgata tcgtagaatt accggtcccg gaaaatctct
    12061 attgcacgat tattcatcca gaaattgaaa tcaatactgc cgatgcccgc aatattcttg
    12121 ctcccaatgt tcctctaaaa gacgccgtaa aacaatgggc taacactgcc ggactggttg
    12181 ccggtctcta tcggaaagac ctaacattga ttagccgctc aatgaatgat tatattatcg
    12241 aacctcagcg ggcgagactt attcctcatt tttatgagat gaaaaaggcg gctctggacc
    12301 gcggcgctct cgggtgcagt atttccggct ccggtccgtc gctttttgcg ctttcaaact
    12361 cacaagaaat tgctcaaaaa atcgccggct ctatgtcagc cgtcttagct aattacaaaa
    12421 tctgcaacca aatctatatc tcgacgatta acaccgaagg tcccaaaatc atcgctcaag
    12481 aaggataaaa tttgctttat tacagtacta ctgatcgctc tcacaaagtt tctctgaaag
    12541 aagccgtgtt gaaaggcatg ccaccggatg gcgggctata tctgccggaa tcattgccgg
    12601 ttatgccgga caaatttttc caagacttgc cggaaatgac ttttcagtcg gtgtcttatg
    12661 aaattgctaa agctctactt gggggcgatg tcccatctga tgtccttcaa aaaattatcg
    12721 ttgaagcttt aaatttcgaa acaccactgg tggctatttc ggaaaagatt aaaacactcg
    12781 aactttttca cggaccaact ctcgccttta aagatgtcgg cgctcgtttt atgtcgcgcc
    12841 tgatggctta ttttattcgt aatgactgtc gcgaactcac gatcttagtt gccacctccg
    12901 gtgataccgg cagtgcggtt gctaatggtt ttttccaggt gccgggcatc aatgtcatcg
    12961 tgctttatcc agccggaaaa attagcctaa ttcaagaaaa acagattgcg accctcggcg
    13021 agaatattac tgccttaaaa gtagaaggca ccttcgacga ctgccagcgt cttgtcaaaa
    13081 ccgctctggt ggatccggag gtgcggagtg tacttacagt tagttccgcc aattcgatca
    13141 acatcgcccg tttaattcct caaacttttt attattttaa cgcaattcgc cagattccca
    13201 attttcattc taggaaaatc gctatctgcg taccgagcgg caatttcggt aacctgaccg
    13261 ccggattaat cgctaaaaaa atgggcttac cggtaatacg cttcatcgcg gctaccaata
    13321 tcaatcacgt cgtgccagat tatctagcga ccggaaaata tgaaccacgc gcctcaattc
    13381 agactatctc caacgctatg gacgttggca atcccagcaa tttcgcccgg atgctggcgc
    13441 tctatgatta ttcggttgag aaaatgcgtc gcgatttggc tggctgttat tttaatgacg
    13501 atgaaactcg cgccgccatc gccgaagtct atcgcaatta cggttatatt ctcgacccac
    13561 atggtgccgt cggctacctt ggcattcaaa attatctcgc caaacatcca gaaattgaca
    13621 ccggaatttt tctggaaaca gctcacccgg cgaagttctg cgacattgtc gaaccagtta
    13681 tcgggacaaa agtccccatc cccgaccgat tgaaaaaatt cctggagcgt ccgattctgt
    13741 cgcgcgctat ttccagcgac tacgacgaat tcaaggatct cctgcgggca tagtcaatca
    13801 accttcagaa ggttgaaaat aaaattgtgg acaataaaat acgtatcgaa aaacctttag
    13861 aagttttttt aaccagttct atcaggtctc gtatcttaca gttttaatct tagcttctcg
    13921 tcaggcttga gaacctaaaa aatggatttt acggttccgt tatcacccgg cagttattgt
    13981 acaaaatctg ccattcacca ctcggagcgc gttgctggat aaaggccgtc tcgcccttca
    14041 tccagaacac gatttcccgt tcatcggtat agcgc
//
LOCUS       DAOOMU010000058        13729 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4877_length_13729_cov_18.692921, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000058 DAOOMU010000000
VERSION     DAOOMU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13729
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4877_length_13729_cov_18.692921"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1048
                     /locus_tag="PLI55_06645"
     CDS             53..1048
                     /locus_tag="PLI55_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020599588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HPB00549.1"
                     /translation="MIIETHAYARAGLLGNPSDGYFGKTISIIVANFGAHVLLYETPE
                     LKIESSDSDRNVYKNAYDLVERIGSLGYYGGDRLIKAAIRKFFTYCMENNIKLDYRNF
                     SIRYNSSIPRQVGLGGSSAIVTATMRALMKFFKVSIPKELLPSLILAAEVDELGINAG
                     LQDRVIQVYEGCVYMDFNREIMEQKNHGIYIPLDKSKLPKLYLAYKTSLSKVSGRALN
                     DIKVRYERGDPEVIACLSRIAELAEEGKAAIENGDEATLNRLINENFDNRKKIMTISE
                     SNQEMIDVARECGASAKFAGSGGSIIGIYKDDAMLNRLIVNLKKINARVIKPYIV"
     gene            1074..1949
                     /gene="galU"
                     /locus_tag="PLI55_06650"
     CDS             1074..1949
                     /gene="galU"
                     /locus_tag="PLI55_06650"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HPB00550.1"
                     /translation="MIKKAVIPAAGLGTRFLPATKAQPKEMLPIIDTPTIQYVVQEAI
                     DSGIDDILIISGKGKRAIEDHFDRNTELEHILEQKGESDLATSISRIGEMANIHYIRQ
                     KQLNGLGDAIYYARQHTGDEPFVVMLGDTINDAVIPVTQQLIDIYNQYHQTIIAVERV
                     PLDKIQMYGIVSGDEVSERVYKVNQLIEKPRPTQTRSNLAVAGRYILTPTIYSMLEKV
                     KPGKNNEIQLTDAIQLLLQRESVYAFIFEGKRYDVGNKLDYLKTTVEMALKREEFAGQ
                     FLDFLKSIVAQAEKK"
     gene            complement(1996..2574)
                     /locus_tag="PLI55_06655"
     CDS             complement(1996..2574)
                     /locus_tag="PLI55_06655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB00551.1"
                     /translation="MDKNTHELFEVTLSETRSHVLASTTNGIVVEMIERLGEIDFGRI
                     VKISETLVDNFGQNARFNRNMIKKYFNYPETLPFVIRYNGEIEGFIIGVPLEYFDKES
                     WAQCDENLGKNNTIYTYAFIVRKEHRALGLSKMLKRVYQNTLKRRGFLYITGHVMQGV
                     TANFTRDYKIVRQFENWNNTGFTFDYYRSALK"
     assembly_gap    2634..2733
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3032..3679)
                     /locus_tag="PLI55_06660"
     CDS             complement(3032..3679)
                     /locus_tag="PLI55_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPB00552.1"
                     /translation="MKYGIIGFSGRMGHEIDELFREKGHECVLLIDDKTEKVESVPEV
                     ILDFSLPSGIEKTITNVRKYQCALITGVTGYSAEQLEAIRALGQEVPVVQSFNFSLGI
                     QLMLKMVNLLKEPLKDWDVEIAETHHRFKKDKPSGTAKMLAATIGREVPTSSFRLGNV
                     LGDHSIAFGSLGEIITLSHRTLSRRAFAEGALLSAEFAIRQKSGYFTFTDVLSQD"
     gene            complement(3676..4554)
                     /gene="dapA"
                     /locus_tag="PLI55_06665"
     CDS             complement(3676..4554)
                     /gene="dapA"
                     /locus_tag="PLI55_06665"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPB00553.1"
                     /translation="MFKGLGTALITPFTENGEVDYDAFRKLIHYQIDGGADCLIVLGT
                     TGEAPAILSHEREKIVQIAIAETNHRIPVIIGTGSNCLDTVLKYNRMAEELGSDGLLI
                     VTPYYNKGTQESIFQYYRYIAERTKLPIIVYNVPSRTGFNLLPDTMFRIFNACPNIIG
                     IKEANADFGQLTKLMAGRPEGMLVYSGNDDITLPFMALGAEGVISVVGNIIPSEMKQL
                     TVRMAADDLAGAREIQNRYLDLINAMFVEVNPIPVKYAASRLNLCQNVLRLPLIPLSE
                     KNAQLVASLMQAKGLI"
     gene            complement(4847..5542)
                     /locus_tag="PLI55_06670"
     CDS             complement(4847..5542)
                     /locus_tag="PLI55_06670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB00554.1"
                     /translation="MPKDFELIIFDMDGTLYPVSAEMDSIYPIVALECAAKSVNRQPA
                     EIKADFEQKRAELARYLNGIPTSTLTLMYFYDADMHAFEMEITRRILTKKYVQPDART
                     IRVIDAIAQFYPIFLFTTNNRLTAEKILDQIRLSEYFPPERRFTFSDINALPISKTEK
                     INFMKPSTKGFEHIMEKCGANPNRTLMVGDSETMDIEPARRLGMEIYHVTEPALLYAL
                     PEWLEIEHNDKKI"
     gene            5795..6496
                     /locus_tag="PLI55_06675"
     CDS             5795..6496
                     /locus_tag="PLI55_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB00555.1"
                     /translation="MMSNNKLLVLIDIDGTLIKSGLTPRKALKQAIFEITGCPVTFED
                     NHLAGLTDPLIITNTLNQLGLGLKDDGLPTKILNRYLEIFSWDYPAAPDKKIYPGAIE
                     LLDYLKTLPVRMGLLTGNAKRGAQIKLAPFGLWRYFDFGVFGDDNANRNELPRLALRK
                     VHELFSESYPPNKVIIIGDTVHDLICAKVNQMQSVIVVRRPEWRLSIEAQKPELLVES
                     FVPLEPVQNWFEKLL"
     gene            complement(6619..7701)
                     /gene="ychF"
                     /locus_tag="PLI55_06680"
     CDS             complement(6619..7701)
                     /gene="ychF"
                     /locus_tag="PLI55_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPB00556.1"
                     /translation="MQIGITGLPYSGKSTLFATLLSRKSESDPFHKKETEKGIVKVPD
                     VRLDRLTEIFNPRRKVPATIEYLKVPGLESQSSAAKGLPPQFLANLKTVDVILLIVRA
                     FDNPLYPHPLNRIDPQGDINFVNSEFLLNDLAIVETRIERLEKQLSKIHNLSFQKELE
                     VLFKCRNFLEDEKPLRSLALLPEEDKILRGFQFLTAKPLIIVLNIDEKEIQQTPEITK
                     RYANLKTVNTTYTVLCAELEKEISELDPAEQPLFLTELGISEPALNRLIRLSYELLGY
                     ISFFTVGEDECRAWTIINGDNAQKAGRAIHSDIERGFIRAEVVHYTTLLENGNLSTCR
                     AKGLIRSEGRDYIVRDGDVIEFRFNV"
     gene            7912..8505
                     /locus_tag="PLI55_06685"
     CDS             7912..8505
                     /locus_tag="PLI55_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPB00557.1"
                     /translation="MKKGWVILIAVVVVVLLIVGAVINIYNNLVSLEESVNQAWAQVS
                     NQYQRRADLIPNLVETVKGYAAHERETLESVIQARANATSLNVTPEVLNDPEAFAKFQ
                     QVQGELSTALSRLMAVAENYPNLKANENFLALQSQLEGTENRIAVERQRFNQAVQTYN
                     TRIRRFPNNIFAGMFGFEKKMYFEAQEGAEVAPKVEF"
     gene            8515..9267
                     /locus_tag="PLI55_06690"
     CDS             8515..9267
                     /locus_tag="PLI55_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HPB00558.1"
                     /translation="MLKIKFRNWRIPALVGLLLFNLTAFGLDVPPLKRHVTDLAGILT
                     PSELNRLEENLIAFENETSNQIAVLIINSLEGEPIEQYSIRVADQWKLGTADRDNGVL
                     LLIAVQDRQMRIEVGYGLEGVLTDLISSQIIRNVIAPEFNKGNYYAGIDQGLRAIMMA
                     TKNEYQADPRQREQYQTERGKSLASLIFFIIFISLFIGGGRRGRRNLLGAIFFSNMFG
                     GGGWRSGGSGGGFGGFSGGGGGFGGGGASGRW"
     gene            9306..9758
                     /locus_tag="PLI55_06695"
     CDS             9306..9758
                     /locus_tag="PLI55_06695"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPB00559.1"
                     /translation="MSLIKKYFSTSDLDAIKKACECAEKNTAGEIRVSIFEKRPPKTS
                     KMSLPELAYAEFKNLRMDQTRDRTGILLFILLAERQFQILADEGINAKVEQEVWDDIA
                     EQMAEKFKNGDYLTGVVSAVKRIGEILAQWFPRKPDDINELSNEVHIS"
     gene            9782..11830
                     /locus_tag="PLI55_06700"
     CDS             9782..11830
                     /locus_tag="PLI55_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00560.1"
                     /translation="MRLSKLNLIFLGLFLSFSLSFLHNCGEKKPEAKMIDIKERTELT
                     NVLVRKFGEENRDRLETGVKQVCDFWRAEDGDLATLKQFCLDNFISDQAELLKAFEHI
                     EKTFETMSGVLVELNRDLRWYMDVPTGPILPIDYLLANFNLGSHVEDDLYATKIAFFV
                     LLNYKVYTLEEILAEGAEWDRQKWAEVRLAQKFTSRVPAQYNQKRYEAYTAADNYITN
                     YNIYMHNLLTEDEQRLFPEGLKLIAHWGLRDELKAQYQLSDGFPRQKLIYEVMLKIIN
                     QEIPKIVIDNPAVDWNVAKNTVSGVSSDNSPEPDTRYARWLGVFQGERACDPYYLISR
                     SQIDRRFNEDREIPEDIVRQLFVDVLTAPELKQTARLISKRLGRELEPFDIWYNQFQA
                     KSRYTEAELDKITQVKFPTAESFKMQLPEILMKLGFDRSTANFLQTKIDVDPARGSGH
                     AMGAARRSDNAHLRTRVTENGMDYKGYNIALHELGHCVEQVFSLNKVDHTLLQGVPNT
                     AFTEAFAFVFQKRDLDVLGLSEKDPQSEHLAALNDLWQTAEIAAVALVDMDAWHWLYE
                     HPEATPKEFKQAVIDIAKRVWNEYYYPVIGKKDSEILAIYSHMIDSGLYLPDYPLGHL
                     ISFQIEQYIKGKNLAREMERMCVQGRISPIYWMEGAVGAKLSAEPMISAAYEALKIIS
                     "
     gene            complement(11881..13266)
                     /locus_tag="PLI55_06705"
     CDS             complement(11881..13266)
                     /locus_tag="PLI55_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HPB00561.1"
                     /translation="MVSKIFQSLLIFLIMATNTFLRGENPSNQTTSDHLQPPVQLTAE
                     QDHQRLMDLLNIKELRPWVNGLDPNAPNLPNYDESKANPYPNLPDPLVLKDGRRVTTP
                     EMWWKERRPEIIEDFDREIYGRVPENIPPVNWVVTAIEPQTVGGISVITKYLVGHVDN
                     SSYPLVNVDIQLTLSTPANATQPVPVIMELSFGNGIPYESTTYQTEPIWQEQVLAKGW
                     GYAILIPTSIQADNGEGLTQGIIGLCNKGQPRKVDDWGALRAWAWGASRALDYFKNDQ
                     SVDAKRVGIEGLSRYGKAAIVAMAYDERFAIGFIGSSGEGGLKLHRRNFGEIVENLAG
                     SGEYHWMAGNFLKYAGPLTWDDLPVDAHELLALCAPRPVFISVGSEQVEGGWVDAKGM
                     FMAGVGAGPVYRLLGKKDLGTTEFPPQGTALIEGEIAFRQHSGGHTTGPNWPTFLSYA
                     ERYFSNQNNIE"
BASE COUNT         3914 a         2826 c         3012 g         3877 t
ORIGIN      
        1 ctaagtatat tatcgttgtg aatctaaata tgataaaaat cggtttaaca taatgataat
       61 cgaaactcat gcttatgcca gagctggatt gcttggtaat ccttccgatg gctatttcgg
      121 aaaaaccatc tcaatcatcg tcgctaattt cggggcgcat gtcttactct acgaaacacc
      181 ggaattaaaa atcgaatcgt ccgattctga ccgaaacgtc tataaaaacg cttatgacct
      241 ggttgaacgg atcggttcgt tgggttatta tggtggtgat cgcttaatca aagccgcaat
      301 tcggaaattt ttcacttatt gtatggagaa taatatcaag ctggactacc gcaatttttc
      361 cattcgttat aactcttcga ttccgcgtca ggttggtctg ggtggttcga gtgcgattgt
      421 tacggctaca atgcgagcgt taatgaaatt ttttaaagta tcgattccta aggaattgtt
      481 accgagcctg attctggcgg cagaagttga tgaactgggc atcaatgccg gtttgcagga
      541 tcgcgtcatc caggtttacg aaggctgtgt ctatatggat ttcaatcgcg aaattatgga
      601 gcaaaaaaat cacggaattt atattccgtt ggataaaagc aaactaccca aactttacct
      661 agcttataaa acttcgctga gtaaagtatc aggtcgagcg ttgaatgata ttaaagtccg
      721 ttatgaacgc ggcgatccgg aagtcatcgc ctgtttaagc cgtattgctg agttagccga
      781 ggaagggaaa gcggctattg agaacggtga tgaggcgact ctgaatcggc tgatcaatga
      841 aaatttcgat aatcgtaaga aaattatgac tattagtgaa agcaatcagg aaatgattga
      901 tgtcgctagg gaatgcggcg cttccgctaa atttgccggc tcaggtggtt ccattatcgg
      961 gatttataaa gatgatgcga tgctcaaccg cttaatcgtt aatctaaaga aaattaatgc
     1021 tcgggtgatc aaaccctaca ttgtttaata ttatttagga ggaaatggat aaaatgatca
     1081 aaaaagcagt cataccagcc gctggattag gaacgcgctt tttaccagct accaaagctc
     1141 aacccaagga aatgttgccg ataattgata ctcccacaat tcaatacgtt gtccaggaag
     1201 ccatagattc tggaattgat gacattctaa ttatttccgg caaaggcaaa cgtgctattg
     1261 aagatcattt cgatcgcaat acggaattgg agcacattct cgagcaaaaa ggagagagtg
     1321 atttggctac cagtatcagc aggatcggag aaatggctaa tattcattat atccggcaga
     1381 agcaactcaa tggtcttgga gacgcaatct attacgccag acaacatacc ggcgatgagc
     1441 ctttcgtagt gatgctcggc gatactatta acgatgctgt cattccggta actcaacaac
     1501 tgattgatat ttacaatcag tatcaccaga ctatcatcgc ggtcgaaaga gtaccgcttg
     1561 ataaaatcca aatgtatggt attgtttccg gagacgaagt cagtgagcgt gtttacaaag
     1621 tcaatcagct gatcgaaaag ccacgtccca ctcaaacccg ttccaatcta gcagtagccg
     1681 gccggtacat tttgactcct accatatact caatgctcga aaaggttaag ccaggtaaaa
     1741 acaacgaaat tcagctgacg gatgcaatcc aacttttatt acagcgcgag tcggtctatg
     1801 cctttatatt tgaaggtaaa cgctatgacg tcggtaacaa gctggattat ttgaaaacaa
     1861 cagtcgagat ggcactcaaa cgcgaggaat ttgccgggca gtttctggat tttctcaaat
     1921 ctatcgttgc tcaggcagaa aagaaataaa atctattcaa aggctttaaa atatttcaat
     1981 taaaacatca aaagattatt ttagtgccga acggtagtaa tcaaaagtaa agccggtatt
     2041 gttccaattt tcgaattgcc ggacgatttt ataatcgcgc gtaaaattag ccgtcacccc
     2101 ctgcattacg tggccggtga tgtatagaaa accgcgacgt ttcaaggtat tttggtaaac
     2161 ccgcttgagc attttggata accccagcgc ccgatgttct ttacgcacga taaaagcata
     2221 agtataaatc gtgttattct tgccgagatt ttcgtcgcat tgcgcccagc tttctttatc
     2281 gaaatattca agcggtaccc cgataataaa accttcgatt tcaccattat agcgaatcac
     2341 gaaaggtagt gtttccggat aattgaaata ttttttgatc atattgcggt taaagcgagc
     2401 attctggccg aaattgtcca ccagtgtttc agaaattttg acaatccgac cgaagtcaat
     2461 ttcaccgaga cgctctatca tttcgaccac aattccgttg gtagtggagg ctaatacatg
     2521 actgcgggtt tcagaaagtg tgacttcaaa taattcgtgg gtatttttat ccatacggcg
     2581 gaattataga cagaaaaatt gaaataatca atggcgagaa tgaattaatt gccnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncggaatt atagacagaa aaattgaaat
     2761 aatcaatggc gagaatgaat taattgccta aatctgaata gtcaatcact tactgtttcc
     2821 attgtacaat gatgatccaa ttgtattgag cgttgttcat tatgatttag tccattgaaa
     2881 acttacgtac gttttctcat tacgattgta ctgactgtaa ttccatatat cgtattcttc
     2941 gccatgaaaa taattttcga ctatgaaaat agtttagcaa ttgaatattt tcggtttgat
     3001 aaagtctaat tggaacccag ccgatctcgc atcaatcctg agacaaaacg tcagtaaatg
     3061 tgaaataacc tgatttctga cgaatggcaa attcggctga tagcaatgcg ccttccgcaa
     3121 atgcgcgccg cgagagcgtg cgatgggaca acgtaatgat ttctcctaaa ctgccaaagg
     3181 cgatcgaatg gtcaccaaga acgtttccca agcggaaaga gctggttggc acctctctgc
     3241 cgatagttgc ggcgagcatt ttggcggtac cgctgggttt atccttttta aagcggtggt
     3301 gagtttcggc aatttccacg tcccagtctt tgagcggttc ttttaacagg tttaccattt
     3361 tcaacattag ctggatgccg agagaaaaat tgaaagactg cactaccggt acttcttgac
     3421 caagagcacg aattgcctcc aattgttccg cgctataacc ggtaactccg gtgatcaacg
     3481 cacattgata ttttctgacg ttggttattg ttttttcaat gccggatggc agtgagaagt
     3541 cgagaattac ctctggcacc gattcgactt tttctgtctt atcatcaatt aggagaacac
     3601 actcatggcc tttttcacgg aaaagttcat ctatttcgtg acccattcgg ccgctaaagc
     3661 caattattcc gtatttcata ttaatccttt tgcttgcatt aagctcgcta ctaattgcgc
     3721 atttttctcc gataacggga ttaacggtaa tcgcagaacg ttttggcaca gattcaggcg
     3781 cgatgccgca tatttaaccg gtatcgggtt cacttcgaca aacatggcat tgatcaaatc
     3841 cagatagcga ttttgaattt cacgtgcgcc agccagatca tcagccgcca tccgtactgt
     3901 caactgcttc atttcggatg gaatgatatt gccaacgaca gagatgacgc cttcggctcc
     3961 gagcgccata aacggtagtg taatgtcgtc atttccggag taaaccagca taccttctgg
     4021 gcgtcctgcc attaacttcg tcaattgacc aaaatcggcg ttggcttctt taattccgat
     4081 gatattggga caagcattga aaatccgaaa catcgtgtcc ggcaggagat tgaaaccggt
     4141 gcgcgacgga acattataga cgatgatcgg taatttggta cgctcggcaa tgtatcggta
     4201 atattgaaaa attgattctt gagtgccttt gttataataa ggcgtaacga tcaataatcc
     4261 atcgcttccc aattcttctg ccatacgatt atacttcaaa acggtatcga gacaattcga
     4321 gccggtccca atgattaccg gaatgcgatg attggtttcc gcgatagcaa tctggacaat
     4381 cttttcgcgt tcatggctca gaatagccgg tgcttcgcca gtagttccca gcactattag
     4441 acagtccgct ccgccatcaa tctgataatg aattaattta cggaacgcat cgtaatcaac
     4501 ctctccattt tccgtgaaag gcgtaatcaa cgctgttcct aatcctttaa acataacttc
     4561 ctcacttttt agatttaaaa aaacaaaaag ggggtctact acttggaccc cctatgtata
     4621 aaaatacatt cattctatta gggggttaca aatcgggttt cttagcgatc ttggcaactt
     4681 taacatagtt gcgtgagaac gccgattttg aattaattat aatgcctcga taaatcattt
     4741 gaattcaatt ttcttttaat taatcacaac acaaataaaa aataaaatag atttaactaa
     4801 gatgcaagta atttttagag atttttttgc caatatattt aataacctaa atttttttat
     4861 cattgtgctc aatttccaac cattccggca aagcatagag taacgccggt tcggtaacgt
     4921 gatagatttc catcccgagc cgtcgggcag gctcgatgtc catagtttcg gaatcgccga
     4981 ccataagcgt ccgattggga ttagcgccac atttttccat gatatgctca aatcctttgg
     5041 ttgacggttt cataaaattg attttttctg tcttgctaat cggcagagca ttaatatcgc
     5101 tgaaagtgaa ccgtcgttcc ggcggaaaat attcagatag ccgtatctgg tcgagaattt
     5161 tttcagctgt aagccggtta ttggttgtaa agagaaaaat cggatagaat tgcgcaatag
     5221 catcgattac ccgaatcgtg cgtgcatccg gctgaacata tttcttagtc aaaatgcgtc
     5281 gggtaatttc catttcaaaa gcgtgcatat cggcgtcata gaaatacatt aaagtcagag
     5341 tgctggtagg gatgccattt aaataacgcg ccaattccgc ccgtttttgt tcaaaatcag
     5401 ctttaatttc cgctggctga cgattgaccg atttagcggc acattccaaa gccacgatcg
     5461 gataaataga atccatttcc gccgaaacgg gataaagagt cccatccata tcaaaaataa
     5521 tcagttcaaa atcttttggc attcgatagc tcaatcatag gaaaatcggc gtggaaatta
     5581 tcattttgag ccgagacctc caattatttt ttaggtataa gctgagcgaa aaaacttgcg
     5641 attttccgga aatattcaga ttttgtcacg gttatttgat ttttaatagt ggaatctgaa
     5701 aattatgcgt cggtcggaat tcatcgtaac attcgaatta attttcattg taaattaaag
     5761 gtagattcta ataactgagt gcgatttaaa ttaaatgatg tccaataata aacttctcgt
     5821 tctaattgat attgacggta cccttatcaa gtctgggctg actcctcgca aagcgctcaa
     5881 acaggctatt ttcgagatta ctggttgtcc ggttaccttt gaagataacc atttagcggg
     5941 tttgaccgat cccctaatta ttactaatac tctcaaccaa ctcggccttg ggcttaagga
     6001 tgatggtttg ccaacaaaaa ttttaaaccg ctatcttgaa atttttagct gggattatcc
     6061 agccgctccg gataaaaaaa tttatccagg tgcgattgaa ctactggatt atctcaagac
     6121 tctaccggtt cggatgggat tattaaccgg caacgctaag cgcggtgcgc aaattaagct
     6181 cgcacctttt ggcctatggc gctactttga ctttggcgtt tttggcgatg acaacgccaa
     6241 ccgtaatgaa ctgccgcgcc ttgcgctgag aaaggttcat gagttattta gcgagagcta
     6301 tccgccgaac aaagtgatta ttatcggcga tacggtacat gacctcatct gtgccaaggt
     6361 caatcaaatg caatcggtaa ttgttgtgcg tcgtccggag tggcggttgt caatcgaagc
     6421 tcaaaaaccc gaattactag tcgaaagctt cgttccgctg gaaccggtgc aaaactggtt
     6481 tgaaaaactg ttgtgactgg atttatttta acaggataaa caagattaac atgattatta
     6541 aatacagatt taatttacca attttcacta atactctgcg ttcttggcgc tctccgcgga
     6601 taaataaggt aaatttcttt aaacattaaa tcggaattcg atcacatccc catctctgac
     6661 gatataatcg cgtccttccg agcgaatcaa acccttagct cgacaagtag aaagattgcc
     6721 gttttccaat aaggtagtat aatgcacaac ttcggcgcgg atgaagccgc gttcgatgtc
     6781 ggaatgaata gcccgtccgg ctttctgagc gttgtcgccg ttgatgattg tccaggcgcg
     6841 gcattcgtcc tcaccgacgg taaaaaatga aatatagcct agcaattcat aagataaacg
     6901 gatcagacga ttgagggctg gttcgctaat tcccagttcg gttaaaaaga gcggttgctc
     6961 ggccggatcg agttcagaaa tttccttttc cagttccgca cataaaacgg tataagtagt
     7021 gttgacagtc tttaagttgg cgtagcgttt agtgatttca ggcgtttgct gaatttcttt
     7081 ttcgtctata ttaaggacaa tgattaatgg tttagcagtt aagaattgga atccgcgtaa
     7141 gattttgtcc tcttccggta aaagcgccag agaacgcagt ggtttttcat cttcgagaaa
     7201 attgcggcat ttgaaaagta cttccagttc tttctgaaag gacaaattat gtattttact
     7261 gagttgtttc tcgagacgtt cgatgcgggt ttcgacgata gccaggtcat ttagaagaaa
     7321 ttcgctattg acaaaattaa tatcgccctg ggggtcaatg cgattcagcg gatgtgggta
     7381 aagcggattg tcgaaagctc tgacgattag cagaatcacg tccacggttt tcaaattagc
     7441 gagaaactga ggcggtaagc ctttagcggc agatgattga ctttccagcc cggggacttt
     7501 cagatattca atagttgccg gaactttacg gcgcgggttg aaaatttccg ttagacggtc
     7561 gagtcggaca tccggcactt ttacgatgcc tttttcggtt tcttttttgt gaaaaggatc
     7621 ggattcgctt ttgcgactta ataaagtagc gaacagggtt gatttaccgg aataaggcag
     7681 tccggtaatg ccgatttgca tctaaactcc aatgaaaata ccattaaaaa acggcggaat
     7741 ttaacttcga atagtcagat caccaaacct gaacgactac cctttaatca acacatcaag
     7801 gtcaatctgg ttcttttata attaatttcc gatttctttt ttagcctggt gattgtcgaa
     7861 tgtgtaaaaa tttcttagat ttaccgagta atttgaaaag gagttaaaaa tatgaaaaaa
     7921 ggttgggtta tcttaattgc tgtggtcgtc gtcgttctac taatagtcgg cgcagtaatc
     7981 aatatttaca acaacttagt ttcgctggaa gaaagtgtaa atcaagcctg ggctcaggta
     8041 agtaatcaat atcagcggcg ggctgatctg attcccaatt tagtcgagac agtcaaaggc
     8101 tatgccgctc acgagcgcga aaccttagaa agtgtcattc aggcgcgcgc taacgctacc
     8161 agcttgaacg ttaccccgga agtgctgaac gatccggaag cttttgccaa attccagcaa
     8221 gtacagggcg agcttagcac agccctatcg cgcttaatgg ccgtggctga aaattatcca
     8281 aacctaaaag ccaacgaaaa tttcttggca ctccaatcgc aattggaagg cactgagaac
     8341 cgtattgcag ttgaaagaca acgcttcaac caggcggtgc agacttacaa tacccgcatc
     8401 cgccgtttcc cgaacaatat ttttgccgga atgtttggat ttgagaaaaa aatgtacttt
     8461 gaagcccagg aaggcgccga agttgccccc aaagtcgagt tttaattacg gattatgctt
     8521 aaaataaaat tccgcaactg gcgaatccca gcgctcgttg gactattgct tttcaacctg
     8581 acggctttcg gactggatgt accgcctttg aagcggcatg taactgacct agccggaatt
     8641 cttactcctt ccgagttaaa tcgcctcgaa gaaaacctga tcgcctttga gaatgaaact
     8701 tccaaccaga ttgcggtttt gattattaat agtttagaag gcgagcctat cgaacaatat
     8761 tcgattcggg tcgccgatca gtggaaactc ggtaccgccg atagggacaa tggcgttttg
     8821 ttgctaattg cagttcaaga tcgccagatg cggatcgaag tcggttacgg tctggagggc
     8881 gtcctgaccg atcttatcag ttctcaaatt atccgcaatg ttatagcacc agaattcaac
     8941 aaaggaaatt attacgccgg aatagatcaa ggcttacggg caattatgat ggctaccaaa
     9001 aatgaatacc aagccgatcc acgacagcgg gaacagtatc aaaccgagcg cggcaaatca
     9061 cttgcctcat tgatcttttt tatcatattt atttcgctct ttatcggcgg tgggcgccgg
     9121 ggaagacgca atctgctcgg ggcgatattt ttttcgaata tgtttggcgg cggtggctgg
     9181 cgttccggtg gctccggcgg tggttttggt ggtttttcgg gaggcggtgg tggatttggc
     9241 ggcggtggcg cttcaggaag atggtaaaac tgtgattaac tgattttcat tttaatgagg
     9301 aataaatgag cctgattaaa aagtatttct cgacgagtga tttagatgct atcaaaaagg
     9361 cttgcgagtg tgccgaaaaa aatactgccg gtgaaataag agtctccatt tttgaaaaaa
     9421 ggccgccaaa aacttcaaag atgagcctac cggaactcgc ttatgcagaa tttaaaaacc
     9481 taagaatgga tcagactcgc gaccgtaccg gaattttgct gtttatttta ttagccgaac
     9541 gccagttcca gattttagcc gacgagggta ttaatgctaa agtcgagcaa gaagtctggg
     9601 atgatattgc cgaacaaatg gctgaaaaat ttaaaaacgg cgattatctt acgggagttg
     9661 tatccgcagt aaaacgaatt ggcgagatcc ttgcccagtg gtttccgcgc aagcctgacg
     9721 atatcaacga actcagtaat gaagttcata tctcttaagt ttaataataa gaggaattaa
     9781 aatgagattg tcaaagttaa acctcatatt tttaggttta tttttatcct tttcattaag
     9841 ctttttgcat aattgcggtg agaaaaaacc ggaggctaaa atgatcgata tcaaagagcg
     9901 caccgagttg acaaacgttt tagtccgaaa attcggagag gaaaatcgcg accgccttga
     9961 aactggcgtc aagcaagttt gcgatttctg gcgggctgaa gacggcgacc tcgccactct
    10021 gaagcaattt tgtctggata attttatttc cgatcaggct gagctattga aagcttttga
    10081 acatatcgaa aaaacttttg aaacgatgag cggtgttctg gtagaattga atcgtgattt
    10141 gagatggtat atggatgtgc cgaccggtcc tattctaccg attgattatt tactggctaa
    10201 tttcaatctc ggcagtcatg tcgaagatga tttatacgct accaaaattg cgttttttgt
    10261 tttactgaac tataaagttt atactctgga agagattctt gctgaaggtg ccgagtggga
    10321 ccggcagaag tgggcagaag tccgtctagc gcagaaattc acttcgcgcg ttccggcaca
    10381 atataatcag aaacgctatg aagcctacac tgccgctgat aattatatca cgaattataa
    10441 tatttatatg cacaatctgc tgacggagga tgaacagcga ctttttcctg aggggctgaa
    10501 attaattgcg cattggggtt tgcgtgatga attaaaggcg cagtatcagc tatctgacgg
    10561 tttccctcgg caaaaattga tttatgaagt aatgttgaaa atcatcaatc aggaaattcc
    10621 aaagattgtg atagacaatc cggcggtgga ttggaatgtc gctaaaaata cggttagcgg
    10681 tgtcagctct gataactcac ctgagccgga tacgcgctac gccaggtggt tgggcgtttt
    10741 ccaaggcgaa agagcctgcg acccgtatta cctaatttcc cgatcgcaaa ttgatcgtcg
    10801 tttcaatgag gaccgcgaaa ttcccgagga catcgtccgg caactctttg ttgatgtatt
    10861 gaccgcgccg gaacttaagc aaaccgcgcg gttgatttcc aagcggcttg gtcgcgagtt
    10921 ggaaccgttc gatatctggt ataaccaatt tcaggctaaa tcaagataca cggaagccga
    10981 acttgataaa attactcagg tgaaatttcc aacggctgaa tcttttaaaa tgcaattgcc
    11041 ggaaatcctg atgaaactcg gtttcgaccg gagtacagct aattttttgc aaactaagat
    11101 tgacgttgac cctgcccgcg gttctggaca tgctatggga gccgctcgtc gttcggataa
    11161 tgcgcatctg cgcacgagag tcacggaaaa tggtatggac tacaaaggtt ataatattgc
    11221 tctccatgag ctcggacatt gcgtcgagca ggttttttcg ctcaataaag ttgatcatac
    11281 tttattgcag ggtgtaccga acacggcttt tacggaagcc tttgctttcg ttttccagaa
    11341 gcgcgacttg gatgtgttgg gcttatcgga gaaagatcct cagagtgagc accttgccgc
    11401 tcttaatgat ctatggcaga cggctgaaat tgcggcggta gcgctggttg atatggatgc
    11461 ttggcactgg ctttatgaac atccggaagc tacaccgaag gaattcaaac aggctgtaat
    11521 tgatattgct aagagagtct ggaatgaata ttattatccg gtaattggca agaaggactc
    11581 agaaatactg gcaatttatt cgcatatgat tgatagcggt ctctacctac ccgattaccc
    11641 gctgggtcat ttgatttcct tccagatcga gcaatatatc aaaggcaaaa atctcgccag
    11701 agaaatggag cggatgtgcg tccaggggcg catcagtccg atttattgga tggaaggtgc
    11761 cgttggggca aaactctccg ctgaaccaat gatctctgcc gcatatgaag ccctgaaaat
    11821 catctcatag accatagcat aatagtaaga aagccctgga tttgctggag gctttatatt
    11881 ttattcgata ttgttctggt ttgaaaagta gcgttcggcg tatgacaaga aagtaggcca
    11941 atttgggcca gtagtatgtc ctccgctatg ttgtcggaaa gctatctccc cctctatcaa
    12001 ggctgttccc tgaggaggga attcggtcgt gccgaggtct tttttaccca gtaaacgata
    12061 gaccggacca gcaccgacac ctgccataaa catacctttg gcatctaccc aaccgccttc
    12121 tacctgttcc gaaccgacac tgatgaaaac cggtcgtggt gcacagagtg ccagcagttc
    12181 atgagcatct accggtagat catcccaggt caaaggaccg gcatatttga gaaaatttcc
    12241 cgccatccaa tgatactcgc ctgagccagc tagattttca acaatctcac cgaagttgcg
    12301 acgatgtaat ttaagtccgc cttcacctga tgaaccaata aaaccaatag caaagcgttc
    12361 atcatatgcc attgcgacaa tagcagcttt tccataacgg gagagtccct caattccgac
    12421 tcgcttagca tcaaccgatt ggtcgttttt aaaatagtca agagcgcggc tggcgcccca
    12481 agcccaagcc cgcaatgctc cccaatcgtc caccttgcgc ggctgtcctt tattgcataa
    12541 accgataatg ccttgagtta aaccttcgcc attatcagcc tgaatgctgg tcggaataag
    12601 aatcgcatat ccccaacctt tagccaaaac ctgctcttgc caaatcggtt cagtctgata
    12661 ggttgtggac tcgtaaggaa taccatttcc gaaggagagc tccataatca caggcacggg
    12721 ctgagtcgca ttagccggtg tgcttaatgt tagttgaata tctacattaa ctaaaggata
    12781 agaagaattg tctacatgtc ccacaagata tttagtaatg acagaaatgc cgccaacggt
    12841 ttggggttca atcgcggtaa cgacccaatt caccggaggt atgttttctg gcactcggcc
    12901 ataaatttcc cggtcaaaat cctcaatgat ttccggacgt cgttctttcc accacatttc
    12961 cggggtagtg actctacggc catccttaag aaccagagga tctggtaaat taggataagg
    13021 attggctttc gattcatcat aatttggcaa attgggggca ttcggatcga gaccatttac
    13081 ccagggtcgg agttctttga tattcaaaag atccattaat ctttggtgat cttgctccgc
    13141 ggtcaattgg accggaggct gtaaatgatc agaagtagtt tgattagatg gattttctcc
    13201 acgcaggaat gtattggttg ccattatcag gaaaattaat aatgattgaa atattttcga
    13261 taccataaac accctttaat ttaatttatt ttcaacttat tcataacatt agtaattcaa
    13321 aaacttacta acctatttga ttactttcgg tttctcatgc tctatgtagt tattataagt
    13381 tatggataaa aggcacaaaa gatgcgaatt atacaaaaaa tagcatgaac gcacaattgc
    13441 atggatttgg ttactagtaa ctggtttctg gcgagcaaga cggtgataaa tataacataa
    13501 cttatgtcac ttgaattaaa aatcggaaaa atcttgttaa ttctgtctaa aaaactttac
    13561 cgaatttgca ggagatgaaa ctatagctcg atgccgagtt cttcaatctt gcgataaagc
    13621 gacgaaaggc cgatgccgag caggtcggcg gctttgtttt tgtcgaaatc aactcgccgc
    13681 agaattccta gaatatgctg gcgttcaaaa ttgcggacgg aagatttga
//
LOCUS       DAOOMU010000059        13724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4882_length_13724_cov_15.248957, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000059 DAOOMU010000000
VERSION     DAOOMU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13724
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4882_length_13724_cov_15.248957"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLI55_06710"
     CDS             <1..630
                     /locus_tag="PLI55_06710"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPB00562.1"
                     /translation="AIYNLALLAYLDSMGYSKRVIKSDHRQLFEKVLTRYENTVRNFI
                     NNSYFKKVQLGKLNVLSDSDALDLKIVDNSIDGVITSPPYSFAIDYAKNDEAQLEYLG
                     YNIEKIKGKMIGLIGKNKDERLNNYFRDMKKVCSEIYRVLKNDKYFVMIIGSNTNQTG
                     GIRLEKTIIQNCEEIGLKLVKSILKPIKGMRNTMKDEYLLFFQKNPDRN"
     gene            633..1622
                     /locus_tag="PLI55_06715"
     CDS             633..1622
                     /locus_tag="PLI55_06715"
                     /EC_number="3.1.21.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009413428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DpnII family type II restriction endonuclease"
                     /protein_id="HPB00563.1"
                     /translation="MKTIDEKYRTVIENNTYYYFDEEFESAYESHIFSVKESIIYLRN
                     EVQNNGCKKEVFENFLRNKKELGLKALLAITGFSNESLLRLITIIRIVDDKELNDFTF
                     MNYWINNKRTTKDNEISEWNTSKIEQLVRENEYFRKCLVNIFYEGSSIDFFKRTLPPF
                     EFQKLSLEKLNFDINAIIDTLARYKEKGSRAAKGKNNPEILIERILKKRKIPFEKGDL
                     SELIEYAPEQKRTMDFIIPNKQSPKIIIESTFLVTTSSGQGDKSKTEISIDSLIKEHY
                     PDARFLGFVDGIGWYVRKRDLKRMVGAYEDVFTFHQEELNRFEKLLCEVMKNV"
     gene            1615..2640
                     /locus_tag="PLI55_06720"
     CDS             1615..2640
                     /locus_tag="PLI55_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPB00564.1"
                     /translation="MFEKYYNQIIQGDCIELFKNIPDNSVDVTFADPPFNLGKKYNGY
                     RDNKEVQAYLDWCKRWIFEMVRITKPAGSIFVHNIPKWLTYYASFLNEIAYFKHWIAW
                     DAPTAPMGQSLQPSHYGILYYVKDLKKNKFFEIRYPHKRCRKCGYLLKDYGGKKAGLH
                     PFGPLVSDVWTDIHRLKHNKYRDEHPCQLPVHLLERIILMSSDEGDIVLDPFVGTGTT
                     AIAAKRLGRKYIGFDIDENYITITQKKLVQEELCSKINGIWLSFFLGEPITIRDKDWV
                     ELAKYFVIPDNIKEIEYSKIKLHPNIFIPETVIKSDLSLFENTESEEEVYKHDLCKVC
                     QSQNIGK"
     gene            2715..4325
                     /locus_tag="PLI55_06725"
     CDS             2715..4325
                     /locus_tag="PLI55_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPB00565.1"
                     /translation="MGEMETLFKNQNNILSLKDASRWASDYLNKKVTESNITYLINYG
                     KINKTSNNGSIYVDISELKNYYDSFWGKREINWKNQLGEDLNWHLSFDFLKEADTTKH
                     VHRLHPYKGKFIPQLVEYFLDDHIDEFKTEVFFNKGDIILDPFCGSGTTLVQANELGM
                     HAIGVDVSTFNALISNVKVKKYDLFSLQAEITKITRSLRVFNSKRNILQFDVELTEKL
                     NEFNNRYFPSPEYKQKVRRKEINEDKYGKTKSDEFLPIYWDLVKKYGVKLKQDTENNF
                     IEKWYLQPIREEMDFVFEQIKLIQDSEIKKIISVIFSRTIRSCRATTHADLVTLIEPV
                     ATPYYCAKHGKICKPLFTILNWWERYSKDTLVRLATFNKLRTNTFQYCLVGDSRNIDI
                     IEKLQSKNKELSDLVQRKKIDGIFSSPPYVGLINYHEQHAYAYDLFGFERNDELEIGP
                     LFKGKGIEARESYVQGITDVLINCKRYLKPNYSIFLVANDKYNLYPMIAEKSGMKIVN
                     CYKRPVLNRSEKDKGAYSEIIFHLKDVQ"
     gene            4322..5368
                     /locus_tag="PLI55_06730"
     CDS             4322..5368
                     /locus_tag="PLI55_06730"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HPB00566.1"
                     /translation="MTNAKIEKIAFEVIKTLKSRFDTFPENEMDNRNAPFHEAFLNAF
                     SVKLEGKVKSVPFLITLSSWLHGLSTTLGQSFFERVSHILSNGEKREFRDLIISSRQE
                     TVISDIITELKNGQRQPDVYSEDNILNYQGEILDRHIPNFTADVFYETDNEIIAIELK
                     TVKPNSGVFKVEKEKILQAKTGLRNKYLGKKVSYYLGFPFDPTSKQPTKSNKERYFGY
                     SIDFRKYFHPQEILLADELWNFLSGETGTMELILSIINSIAKPDFMNKFDIINQPILQ
                     IATKSKAILHDWFLFTELQIIQNFPHLNDLSIKDKTLKRILAQCPFSPDGKINTNRLD
                     IIRPFLEGGEQNAK"
     gene            5358..5498
                     /locus_tag="PLI55_06735"
     CDS             5358..5498
                     /locus_tag="PLI55_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00567.1"
                     /translation="MRSDKIIYSINIADVQTVAEQELDRKLTQEELKFIADEALSTDY
                     FG"
     gene            5564..6202
                     /gene="nth"
                     /locus_tag="PLI55_06740"
     CDS             5564..6202
                     /gene="nth"
                     /locus_tag="PLI55_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPB00568.1"
                     /translation="MTNKSEIAPEIFSRLSAAYPDARCALNHTNPRELLIATILSAQC
                     TDKRVNMVTPALFQKYPTAQAFADADLEELKNDIRSTGFYNNKAKAIKSAMKSVVEKF
                     NGEVPGTMEELLQLEGVGRKTANVVLGDAFGVPAIVVDTHVKRLANLLGLTKNSNPEK
                     IEQDLMRQFPQEQWTKLGHLLIEHGRQVCIARRPQCDKCVLNDICPSARITK"
     gene            6199..6690
                     /locus_tag="PLI55_06745"
     CDS             6199..6690
                     /locus_tag="PLI55_06745"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HPB00569.1"
                     /translation="MNNLSQVSDSIFNNIRSILEDARSRAYRSVNFIMVEVYWNIGRI
                     IVDEEQKGKRRAEFGKQLIADLSRKLTVAYGRGFDPSNLWYMRKFYLLFPILDAVRRE
                     LSDSLANGDSKSVIRLGNSIRPELTWTHYRLLLRIENPQARSFYMNESANNGWSTREL
                     DQW"
     gene            6773..>7290
                     /gene="folE"
                     /locus_tag="PLI55_06750"
     CDS             6773..>7290
                     /gene="folE"
                     /locus_tag="PLI55_06750"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPB00570.1"
                     /translation="METEEIIRNLLINIGENPNRPELLKTPARVAKFWQHVSQGYKVD
                     VLEMLKESLVYEDYNQMIIVKDIDFFSLCEHHLVPFFGKAHVAYIPNGKIIGFSKIPR
                     VVTAFSQRLQIQERLTQQIADALNEALEPKGVGVIIEARHLCMQMRGVEKQNSNITTS
                     VMLGELHDEPQAR"
     assembly_gap    7291..7390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7506..8285)
                     /locus_tag="PLI55_06755"
     CDS             complement(7506..8285)
                     /locus_tag="PLI55_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00571.1"
                     /translation="MIKSIHPKESSTLLGFKKLLSIKRMKYYSPFLIIFIVLASVLSL
                     AAEIEKYYGKIDPGDGHIYTCSGEIIRNDGKVTKTSYFYDENGKILLNEKVVFDEKGY
                     KLISLVSEEPRYGRIQEIQRKGSQYIARYRKNSGEEFREYIINDQPLLLHSSNLPVYL
                     SQNLNLIGESGHVCRLIIVPMKMEIEMIFKNKGIKNIDGHQCFEIQMDAANIFLKPLV
                     KTHYFYYGTEAPHYLYHYIGTISLTNPNGDDIWGTISLTYE"
     gene            8451..>9654
                     /locus_tag="PLI55_06760"
     CDS             8451..>9654
                     /locus_tag="PLI55_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HPB00572.1"
                     /translation="MTPWDFTLNFKDMILDFTALGTFLVLGTIARRYIKFFQKYLVPN
                     NIIGGFLGLLLSQQVLGLIYLDENRLGQYVYHLLALTFIVIGLGKSKTKFGKGPVSKC
                     FTELTCYIVQGAIGLLVVLALIYTVSPDLPAAMGLLVPLGFGMGPGIAYTMGHSWEKF
                     GFEGGGMIGLTFAAIGFLIAYFAGVAIVNRGIKNRETTLIKGIESINNDIRTGVVKEN
                     KPEVAGWLTLSPEAIEPMAFQLGLISIVYAMTYGVIYFVTKLMATYGLKDFVDTIWSF
                     HFVIALLIALLVRKVLDLTDKSYVIDSGLMNRISGVAVDFLVTGSIVAINIVIVGKYW
                     LPITLISILAGIATYFVIKYTSRRAFDDFHFERMVGIFGEMTGTINSGLVLIRITDPE
                     FQTPSAEDL"
     assembly_gap    9655..9754
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9755..10003
                     /locus_tag="PLI55_06765"
     CDS             <9755..10003
                     /locus_tag="PLI55_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00573.1"
                     /translation="VLIRITDPEFQTPSAEDLALGGGLALFLGFPLLILLNAPMNFWH
                     NSLGGYWLTLALMLVYLVILWVVWRLVGYIHFKLPENK"
     gene            10192..11109
                     /locus_tag="PLI55_06770"
     CDS             10192..11109
                     /locus_tag="PLI55_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HPB00574.1"
                     /translation="MLNRYKILDKRIVLNGGEDASILVFINPDEAEKRMLVDQFMIDE
                     HTLNSALDPDELSRLEYEPNHIAFIFKRPRSITAKENILFKVTSTGVFLFKDQIIIVA
                     SEDIPIFEGKIFNRVNSNQDILLKLILRAIYHFLEHLKAINMVTDEFQEKINTSLENK
                     YLIDLFSLAKSLVYYLNAINSNGVLLEKLKINTVKIGFSQEECELLDDIIIENTQCVK
                     QAEIQSNVLASLMDARASIVSNNLNVLMKTLTIITIGIMVPTLIVSIFSMNVDLPLPR
                     NHPSSFWIIIGMALVSVVAFFFFWKRKKW"
     gene            11281..>13724
                     /locus_tag="PLI55_06775"
     CDS             11281..>13724
                     /locus_tag="PLI55_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB00575.1"
                     /translation="MILRNVKVLSITLSVLVILISSGNLMAQPPAGAPRPTSGRLSGT
                     VLIKNTDKPIEYAAITLHGLQDSSIITGTITNEDGIFILDKIPFGRYFAKISFIGYKT
                     AVINNIAITPPKPEVVLGTVYLEPVAIDLSSVTVTGEKKALAYNLDKKVITIDNSVGT
                     IGGTATDIMQTIPSVTVDIDGNVSLRGSSSVTLLVDSRPSQIISLDEIPASMVERVEV
                     ITNPSARYDPEGMSGIINIVLKKKRSPGYNGMLLVNAGTGGKYNGSINLNYRYNKFNL
                     FANYDMRMGHRTGTGETIRESIRNDSTFYLNQYQNSENRNRFHNIKLGGDYFINDYNT
                     ISLSMLYNLRSFSNQNNTEYLNTDYNAVGYNQFTRLSDGDTDNQGQEYGLFYKKTFPQ
                     PQREWTIDLFYSTFSRNSDQSITQQAVLTDSLITVLPDLENIFSDDANRVITARTDYV
                     HPIGEHRRIEAGYRGTFRHNDSDYRRFNYNYTMADWFPDSLASNHYIYDANIHAVYGI
                     YSRELGRLQSQLGLRIEQFNTNNNLVTIDSSFQKNYFSMFPTIHFRYALTENQGLQVS
                     YSRRVHRPHTWQVSPFVDYSDPYNLSSGNPKLKPEYIDVAEIEHSYENRKTSINTSLF
                     YRQVNGMITRLTELQEGGITFTTYQNLNNGVSYGIESVISHNLTPWWRINTNISYFNN
                     RLYGTGISIADANESQSWNARVNSFFRLGENTEMQIMLFYFSPTVGVSGGGRGFHGPG
                     QGRMQENYFVNLGAKHNFLDGKMSAFVRVSDIFKTQKHGQTIYGDNFKTTSLRHPESQ
                     VIFFGISYKINEGIKER"
BASE COUNT         4366 a         2474 c         2645 g         4039 t
ORIGIN      
        1 gccatatata atttagcttt attggcttac ttggattcaa tggggtattc aaaacgtgtc
       61 attaaatcgg atcacaggca attgttcgaa aaagttttaa ctagatatga aaataccgta
      121 cgaaatttta ttaataattc ctatttcaaa aaagtacaac tgggtaaact gaatgtatta
      181 tcggattcgg atgctttaga tttaaaaatc gtggataact caatagatgg cgtaattaca
      241 tctccccctt attctttcgc gattgattat gccaagaatg atgaagccca attagaatat
      301 cttgggtata atatcgagaa aattaaaggt aagatgattg ggttaattgg caaaaataaa
      361 gacgaaagat taaataatta ttttcgtgat atgaaaaaag tctgttcaga aatatatagg
      421 gtgctgaaaa atgacaaata tttcgtaatg ataattggtt cgaatacaaa tcaaaccggc
      481 gggataagat tagaaaaaac tataatccaa aattgtgaag agattggatt aaagttagtt
      541 aaaagcatcc taaagccaat taaaggaatg agaaatacca tgaaagacga gtatttactt
      601 ttcttccaga aaaatccaga taggaactaa atatgaaaac aatcgacgag aagtatagaa
      661 cagtaattga gaataataca tattattatt ttgatgaaga attcgaatca gcctatgaaa
      721 gtcatatttt ttctgtcaag gaatctataa tatatctaag aaatgaagta cagaataatg
      781 gttgtaaaaa agaagtcttc gaaaattttt taaggaataa aaaagaactc ggccttaagg
      841 cattactcgc cattacagga ttctcaaatg aaagtttatt aaggttgatc actattatca
      901 gaattgtgga cgataaagag ctgaatgatt ttacttttat gaattattgg attaataata
      961 aaagaacgac caaagataat gaaatttcgg aatggaatac ctccaaaatt gagcaattgg
     1021 tgcgggaaaa tgaatatttt agaaaatgtc tggtcaatat tttttatgaa ggttcgtcaa
     1081 tagacttttt caaaagaacc ttaccgccgt ttgagtttca aaaattaagt ctcgaaaagc
     1141 taaattttga tatcaatgca atcattgata ctcttgcacg atataaagaa aaaggttcga
     1201 gagctgctaa agggaaaaat aacccagaaa ttcttatcga aagaattcta aagaaacgta
     1261 aaattccttt tgaaaaaggt gatctcagcg aacttattga atatgcaccc gaacaaaaga
     1321 gaacaatgga ttttattatt ccaaataaac aaagcccaaa aattataata gaaagtactt
     1381 ttttagtcac aacttcttca ggtcaaggtg ataaatcgaa aacagagatc tcaattgact
     1441 ctttaattaa ggaacattac ccagatgctc gctttttagg ctttgttgac ggtattggtt
     1501 ggtatgttag aaaaagggat ttaaaaagga tggttggcgc ttatgaagat gtatttacct
     1561 ttcatcaaga ggaacttaat agatttgaga aattattatg tgaagtgatg aaaaatgttt
     1621 gagaaatatt acaatcagat aattcaaggc gattgtattg aattattcaa aaacatcccc
     1681 gataacagtg ttgatgtaac tttcgcagac ccgcctttta atttaggtaa gaaatataat
     1741 gggtatcggg acaataaaga ggttcaggca tatttggatt ggtgcaaacg gtggatattt
     1801 gaaatggtta gaattacaaa acctgcgggt tctatatttg tgcacaatat acccaagtgg
     1861 ttaacatact atgcaagttt tttaaatgaa attgcttatt tcaaacattg gattgcctgg
     1921 gatgcaccaa ctgccccaat gggtcaaagt ttacaacctt ctcattatgg tattttatat
     1981 tatgtaaaag acctaaagaa aaataagttt ttcgaaattc gctaccccca taaacgatgc
     2041 agaaaatgcg ggtatctctt aaaagattat ggcggcaaaa aggctggttt acaccccttt
     2101 ggtcctttag tttctgatgt gtggaccgat attcatcgcc taaaacataa taaatataga
     2161 gatgaacatc cctgtcagtt accggttcat ttattagaaa gaataatatt aatgtcctcc
     2221 gatgaaggtg acattgtgct cgatcctttt gtaggcacag ggacgacagc aattgcggct
     2281 aaacgacttg gtcgaaagta tattggcttt gatatagatg agaactatat aaccattact
     2341 caaaaaaaac ttgtacagga agaattatgt tctaaaatca atggaatttg gttaagtttt
     2401 ttcctaggag agccaatcac aattcgagat aaagattggg tagagttagc aaaatatttt
     2461 gtaattcctg ataacattaa ggaaatagaa tattccaaaa taaagctcca cccgaatatt
     2521 tttattcctg aaaccgtaat taagagtgat ctatctcttt tcgaaaacac tgaatcagaa
     2581 gaagaagttt ataaacatga tttatgtaaa gtatgtcagt cacaaaacat cggaaaatga
     2641 cagttttaga tctgaaaagc ccttgttccc cctccgaaaa aggcaaatta caagggtgga
     2701 aaaggtgaat tgaaatgggt gaaatggaaa cattatttaa aaaccaaaat aatattcttt
     2761 cgctaaaaga tgccagccgt tgggcaagtg attacttaaa caaaaaagtc accgaatcta
     2821 acatcaccta tttaattaac tatggaaaaa taaataaaac cagtaataat ggctcgattt
     2881 acgttgacat ttccgagctg aaaaactatt acgactcgtt ctggggtaag cgtgaaatta
     2941 attggaaaaa tcagttagga gaggatttaa attggcattt gagttttgat ttcttaaaag
     3001 aggccgatac caccaaacac gttcatcggc ttcacccata caaagggaaa tttattcccc
     3061 aactagtaga atattttctt gatgatcata ttgacgagtt taagacagaa gtttttttta
     3121 acaaaggcga cataatcctt gatccattct gcggaagtgg aaccacttta gttcaagcca
     3181 atgaattagg aatgcacgct ataggagttg atgtttctac ttttaatgcc ttgatcagta
     3241 atgtaaaagt taaaaagtat gatttgttct cgctacaagc agaaattacg aaaataacac
     3301 gatcactccg ggtttttaac tcgaagagaa atattctgca gtttgatgtt gaactgactg
     3361 aaaaactcaa tgaatttaat aatagatatt tcccttcacc tgaatataaa caaaaggtca
     3421 gacgtaaaga aatcaatgag gataaatacg gaaaaactaa atcggatgaa tttctaccaa
     3481 tctactggga tttagtaaaa aaatatggcg tcaaattaaa acaggatact gaaaataatt
     3541 tcattgaaaa gtggtacctt cagccgattc gagaagaaat ggattttgtt tttgaacaaa
     3601 ttaaattaat ccaggattca gaaataaaaa agataatcag tgttatattc agtagaacaa
     3661 ttcgctcctg ccgcgctacg acccacgctg atttagtgac cttaattgaa ccggtagcaa
     3721 caccttatta ttgtgccaag catggtaaaa tatgcaaacc actttttacc atacttaatt
     3781 ggtgggaaag atatagtaag gatactttgg tgagattggc aacttttaac aaattaagaa
     3841 ccaatacctt ccaatactgt cttgtgggcg attcaagaaa catagatata attgaaaaac
     3901 tacaaagcaa gaataaagaa ttgtcggatt tagtacagag aaaaaaaata gacggcattt
     3961 tttcaagtcc accatacgtc ggtcttatca attatcatga acaacatgcc tacgcctacg
     4021 atttatttgg ctttgagaga aatgacgaat tagaaattgg tcctttattt aaagggaaag
     4081 gaatagaagc tcgagaatcg tacgttcagg gtattacaga tgtattgata aattgtaaga
     4141 gatatttaaa acccaattat agtatttttc ttgtggctaa tgataaatat aacctatatc
     4201 ctatgattgc cgaaaaatcc gggatgaaaa tcgtcaactg ttataaaagg cctgttttaa
     4261 atcgttcgga aaaagataaa ggtgcctatt cagaaattat ctttcatttg aaggacgttc
     4321 aatgacaaat gctaaaattg agaaaattgc ttttgaggta ataaaaaccc tcaaatcccg
     4381 ctttgatact tttccagaaa atgaaatgga caatagaaat gcgccttttc atgaagcttt
     4441 cttaaatgct ttttccgtta aattagaggg aaaggtaaaa tctgttcctt ttttgataac
     4501 ccttagtagt tggttacatg ggttaagtac aactcttggt caatcattct ttgaacgagt
     4561 atcacatatt ttatctaatg gtgaaaaaag agaattccgg gacctaatta taagtagtag
     4621 gcaagaaaca gtaatttcag atattattac tgaacttaaa aacggtcaaa gacagcctga
     4681 tgtttattca gaagacaata ttcttaatta tcaaggtgaa atattagatc gccacatacc
     4741 aaattttacg gccgatgttt tctatgagac tgataatgaa ataattgcta tagagttaaa
     4801 aacggtaaaa ccgaacagtg gtgtttttaa agtagaaaaa gaaaaaatat tacaggcaaa
     4861 aactggtctt agaaataaat atttaggcaa aaaagtgtct tattacttag gatttccatt
     4921 tgatccgaca agtaaacaac caacaaaatc aaataaagaa agatatttcg gatattctat
     4981 agattttaga aagtatttcc atccacagga gatattactt gctgatgaat tatggaattt
     5041 tctttctgga gaaacaggaa caatggaact gattctctca ataatcaatt caattgcaaa
     5101 acctgatttc atgaataaat ttgatataat aaaccagcct attttacaaa ttgctaccaa
     5161 aagtaaagca attttgcacg attggttttt gtttactgaa ttacaaatta ttcaaaattt
     5221 tccccacttg aatgatcttt caataaagga taaaacttta aagagaatac tggctcagtg
     5281 cccattctca cctgatggta aaatcaatac aaatcgtttg gatattatca gaccattttt
     5341 agaaggaggt gagcaaaatg cgaagtgata aaataatcta ttcaatcaat attgcggacg
     5401 ttcagactgt tgccgaacag gaattagata gaaaattaac tcaagaagag ttaaagttta
     5461 ttgcagatga agctttatct actgactatt tcggatgata cgaaggaatt gttgaaattt
     5521 ttcagcatct aaaattaatg taactaaatc taagaggaaa attatcacta ataaatctga
     5581 aatcgcacca gaaattttct cccgcctttc cgccgcctat ccggacgctc gctgcgcttt
     5641 gaatcatacc aatccgcggg agctactaat tgccaccatt ctatcggcgc aatgcaccga
     5701 taagcgcgtc aatatggtaa caccggctct tttccagaaa tatccgacgg cgcaagcctt
     5761 tgccgatgct gacctcgaag aactgaaaaa cgacatccgc tcaaccggct tttacaataa
     5821 caaagccaaa gccattaaat ccgcgatgaa atctgtcgtc gagaaattca acggcgaagt
     5881 ccccggaaca atggaggaac tcctgcaact cgaaggcgtc ggacgcaaaa cggctaatgt
     5941 ggtgctcggc gatgctttcg gagtaccggc tatcgttgtt gacacccacg tcaagcgtct
     6001 ggcgaatctg ctcggactaa cgaaaaacag caacccggaa aaaatcgaac aggacttgat
     6061 gcgccaattt ccccaagaac aatggaccaa gctcgggcac ctgctaatcg aacacggtcg
     6121 gcaggtttgc atcgcccgac gcccacaatg tgacaaatgt gtcttgaatg atatttgccc
     6181 ctcagcaaga attactaaat gaataatttg tctcaagtct ccgattctat tttcaataac
     6241 atccgaagta ttttagagga tgcccgttcc agagcttacc gatcggtcaa ttttatcatg
     6301 gttgaagtct attggaatat cggacggatt atcgttgatg aggaacaaaa aggtaaaaga
     6361 agggcggaat ttgggaagca actaattgcc gacctttcca gaaaactaac ggtcgcatac
     6421 ggtcggggat ttgatccaag taacttgtgg tatatgcgca aattttatct cctatttcca
     6481 attctcgacg cagtgcgtcg agaattatca gatagtcttg ctaatggaga tagcaaaagt
     6541 gttataagat tgggtaattc gatccgccca gaattaacct ggactcatta tcgcttgtta
     6601 ctaagaattg agaatcccca ggcgcgctcc ttctatatga atgagtcggc caacaatgga
     6661 tggagtacgc gtgaattgga tcaatggtga aattctaagt caaaataaat catttgaatt
     6721 agagcaaaaa ttacaacaat taaaatctga ataaacatta aactgaggtt atatggaaac
     6781 tgaagaaatc atccgcaatt tactcatcaa tattggtgaa aaccccaacc ggccggaact
     6841 gctgaaaacg cccgcgcgcg tcgccaaatt ctggcaacac gtttcgcaag gctataaagt
     6901 ggacgtgctt gaaatgctca aagaatcact cgtttacgaa gattataacc agatgatcat
     6961 cgtcaaagat atagatttct tcagcctttg tgaacatcac cttgtaccat ttttcggcaa
     7021 agcccacgtt gcttacattc cgaatggaaa aatcatcggc tttagtaaga ttccacgcgt
     7081 cgttacggct ttttcgcaac ggctacagat tcaggaacgc ctgactcagc agatcgctga
     7141 tgccctcaat gaagcgctcg aaccgaaagg tgtgggcgtc attattgaag cgcgccacct
     7201 ttgcatgcaa atgcgcggcg tcgaaaaaca aaattccaat atcactacca gcgttatgct
     7261 cggtgaactg catgatgaac cgcaagcgcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn ccaatatcac taccagcgtt atgctcggtg aactgcatga tgaaccgcaa
     7441 gcgcgtgctg aattcttgaa attgattagc cggtaaaaat cgtaacgaat tactcagcgc
     7501 acgttttatt cataagtaag tgaaatcgtt ccccaaatgt catcaccgtt aggattagtg
     7561 agtgaaattg tgccgatata atggtataaa taatggggcg cttccgtacc gtaataaaaa
     7621 taatgagttt taaccaatgg ttttaagaat atattagcgg catccatctg aatttcaaag
     7681 cattgatgcc cgtcaatatt ttttatccct ttgtttttaa atatcatttc gatttccatc
     7741 ttcatgggga ctataatcaa acggcagacg tggccacttt ccccaattaa attgagattt
     7801 tgtgacagat aaaccggcag gttagaacta tgcaagagca acggctgatc attgatgata
     7861 tactcgcgga attcctcgcc gctgtttttg cgatagcggg caatgtactg actccctttt
     7921 cgctgaattt cctgaatgcg accatagcgc ggttcctcgg atacgaggct aatgagtttg
     7981 tatccttttt cgtcgaagac cactttttcg ttgagaagaa tcttgccatt ctcgtcataa
     8041 aagtaagaag ttttagttac tttgccatca ttacggatga tttctccact acaggtgtag
     8101 atatgaccgt cgcccgggtc aattttccca taatattttt caatttcagc agctaaagat
     8161 aaaacggatg caagaacgat gaaaattatt agaaacggcg aatagtactt cattcttttt
     8221 atagataata actttttaaa gcccaagagg gttgaacttt ctttaggatg tattgactta
     8281 atcatcattt tataattttc tactaaaaag taatttttca aacgaaatag aaattatcaa
     8341 aatcagtccc tatagagcaa aaatatttta aattgatttt ataaattatt ttagattact
     8401 atattaaaag tataagagac ggttaattaa aatgatgaaa agaggaaatg atgactcctt
     8461 gggattttac tctgaatttc aaagatatga ttttagattt tactgctctg gggactttct
     8521 tagttctagg aacgatcgcg cgacgctata ttaagttttt ccagaaatat ctggttccga
     8581 ataatatcat tggcggtttt ttaggacttc tgcttagtca gcaagtattg ggattgatat
     8641 acctcgacga aaaccggctg gggcaatatg tttaccatct cctggcgtta actttcattg
     8701 taattggttt aggaaaatcc aagactaaat tcggaaaagg accggtcagt aagtgcttta
     8761 cggaattgac ctgttatatt gtccagggag caatcggtct attggtggtt ctggcattaa
     8821 tctataccgt ttctcccgac ttaccagccg caatgggttt attggtaccg ctgggattcg
     8881 gtatgggtcc gggaatagct tatactatgg gacacagctg ggaaaaattc ggatttgaag
     8941 gcggcgggat gatcggtttg acttttgccg ccattggttt tttgattgct tattttgccg
     9001 gggtggcgat tgtcaaccgg ggtatcaaaa accgcgaaac gactctcatt aaaggaattg
     9061 aatctatcaa taatgatatt cgaaccgggg tcgtcaaaga aaataaaccg gaagttgctg
     9121 gttggttgac attatcgccg gaagctattg aaccgatggc tttccagctt ggactgataa
     9181 gtattgtgta tgcaatgacc tatggggtaa tttatttcgt cactaaattg atggctacgt
     9241 atggtttaaa ggatttcgtt gatacaatct ggtcttttca ctttgtgatt gctctgctca
     9301 tcgccttgct agttcgtaaa gttcttgatc tgactgacaa aagctatgtt attgacagcg
     9361 gtctgatgaa tcggatttcc ggtgtggcgg ttgattttct ggtgactggc tcgattgtag
     9421 ccatcaatat cgtaattgtc ggtaaatatt ggttaccgat tactcttatt tcaattttag
     9481 ctggaattgc aacttatttt gttattaaat acacttcccg acgagctttt gatgactttc
     9541 attttgaacg gatggtcggg attttcggtg aaatgaccgg aacgatcaat tctggtttgg
     9601 tgctgattcg tatcaccgat ccggaattcc aaacgccgtc cgccgaggac ttggnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtgctg attcgtatca ccgatccgga
     9781 attccaaacg ccgtccgccg aggacttggc tctcggtggc gggctcgctc tatttctggg
     9841 ctttccgtta ttgattttac tcaatgctcc tatgaatttc tggcataact ccctcggggg
     9901 atattggttg actctggcgc ttatgctggt ttatctggtt attctctggg tggtatggcg
     9961 cttggtagga tatattcatt ttaaattacc ggagaataaa taaagcgcgg ctacggttcg
    10021 ggagagagta cagcgacagc taattttgct cgtcaatttg ccctcgaacg cagagattga
    10081 attttaataa aaaatttttc tttgttagcc aataattgct taagttcgcc gtgctttcta
    10141 aaaagaatat ttcataagaa tcaacgagca gatttcagac aagaggtaaa aatgctcaat
    10201 cgttacaaaa tcttagacaa gcgaattgta ctgaatgggg gcgaggatgc ttccattctg
    10261 gtttttatta atcccgatga agcggaaaaa agaatgttgg ttgatcaatt tatgatagat
    10321 gagcatactc taaactccgc tctcgacccc gatgagctct cgcgtctgga gtacgaaccc
    10381 aaccacatcg cttttatttt caagcgaccg cgcagtatca ccgctaagga aaatattctt
    10441 ttcaaagtta cttcgaccgg cgtatttctt tttaaagatc aaattatcat cgtggcatcg
    10501 gaagatatac cgatattcga agggaaaatt tttaatcggg tcaactcgaa tcaggacatt
    10561 ttactcaagc tgattttacg ggcgatttac cattttctgg agcatttaaa agccatcaac
    10621 atggtcacgg atgaattcca ggagaaaata aacacttcat tagaaaataa atacctaata
    10681 gacttgttta gtctggcaaa gagtttagtc tattatctta atgccatcaa ttccaatggc
    10741 gttctccttg aaaaactaaa aatcaatacc gtcaaaattg gattcagtca ggaagaatgc
    10801 gaattgcttg atgatattat aatcgaaaat actcaatgtg ttaaacaagc cgaaatccaa
    10861 tcgaatgttt tagccagctt aatggatgcc cgcgcttcaa tcgtcagcaa caatctcaac
    10921 gtcttgatga aaaccttgac tattattacg atcggtatta tggtgccgac gctgatcgtc
    10981 agcatttttt ccatgaacgt tgatctaccc ttaccaagaa atcatccttc ttctttttgg
    11041 ataattatag gaatggcttt ggtttcggtt gtagctttct ttttcttctg gaaacggaaa
    11101 aaatggtaag cctgacgtat tcttgttaaa aacctcttga atccagttaa cttttctaaa
    11161 aatgctaccc agttgtaccg caagcttcta ttccggctaa taattctaaa gtatgtttaa
    11221 cctttttact ttttcggaat caaatagccg aacaatttta accaaaggat tttacgattt
    11281 atgattttgc gaaatgttaa ggtactatct attactcttt cagtactggt aattttaatt
    11341 agtagtggca atctaatggc tcaaccacca gccggtgctc ctcgaccaac atccggcaga
    11401 ctttccggga cggttttaat taaaaatact gataagccga ttgaatatgc ggcaatcact
    11461 ttacacggtc tccaggattc cagcattata accggtacga ttaccaacga ggatggaata
    11521 tttatcctcg ataaaattcc gttcggtcgt tactttgcca agattagttt tatcggatac
    11581 aaaacggcgg tcattaataa tattgcgatt acaccgccga aaccggaggt ggttttggga
    11641 acggtctatc tcgagccggt ggcaattgat ttatcgtccg ttactgtcac tggagagaaa
    11701 aaagccctcg cgtataatct cgataaaaag gtcatcacta ttgataatag cgttggaacc
    11761 atcggaggca cagccaccga tattatgcag actattccgt cggtcaccgt tgatattgat
    11821 ggaaatgtca gtctgcgagg ttcatccagt gttaccttgc tggttgatag ccgcccgtca
    11881 cagattatca gcctcgatga aattccagct agtatggttg aacgagtaga ggttatcacc
    11941 aatccttcag cacgctatga tccggagggt atgtccggta ttattaatat tgttctcaag
    12001 aaaaaacgct cgcccggcta taatggtatg ttactggtaa atgccggaac tggcggcaaa
    12061 tataacggtt ccattaacct taattaccgc tataacaaat tcaatttatt tgctaattat
    12121 gacatgcgga tgggtcatag gaccggaact ggcgaaacta tccgtgagtc aatccgcaat
    12181 gattctactt tttatctaaa ccaatatcag aattccgaaa atcgtaaccg ttttcacaac
    12241 atcaagctgg gtggtgatta tttcattaat gattataata ctatttcact atcaatgctc
    12301 tacaatctgc gctctttctc taatcaaaat aacactgagt atctcaacac tgattataat
    12361 gctgtgggat acaatcagtt cactcgcctc tctgatggtg acacggataa tcaaggtcag
    12421 gaatacggtt tattttataa aaaaactttt cctcaacctc agagggaatg gacaattgac
    12481 ttattttatt caacattctc acggaattct gaccaatcta ttacccagca agcggtttta
    12541 acggatagtc ttataactgt tctaccagat ttggaaaaca ttttttctga cgatgctaat
    12601 cgggttataa ccgcccgcac tgattatgtg catcccatag gcgaacatag acggatcgaa
    12661 gcaggctacc gcggtacttt tcggcataac gattcggatt accgacgctt taattataat
    12721 tatacgatgg cggattggtt tccggatagt ctggcttcca accattatat ttatgatgca
    12781 aatattcacg ccgtttatgg gatttattcg cgggaattag gccgtttaca atctcagtta
    12841 ggtctaagaa tcgagcagtt taataccaat aataacttag tgacgattga ctcgtctttc
    12901 cagaaaaact acttcagtat gtttccaact attcattttc gctacgctct gacggaaaat
    12961 cagggtttgc aggtcagtta cagccgtcgg gttcaccgac ctcatacctg gcaggtttct
    13021 ccttttgtag attattccga tccttataat ctctcttccg gtaaccccaa actcaaaccg
    13081 gaatacattg acgtcgccga gattgagcat tcttacgaaa atcgaaaaac ttcgatcaac
    13141 acctcgctat tttatcgtca ggtaaatgga atgataactc gcctcacgga attacaagaa
    13201 ggcggtatca cttttactac ttaccaaaat ctaaataacg gtgtctctta tggcatcgag
    13261 agcgtcatca gtcataattt aacaccctgg tggcggatca ataccaacat tagctatttt
    13321 aacaaccgtc tttatggaac cgggatttcc atcgccgatg ccaatgagag tcagagctgg
    13381 aatgcccggg tcaattcctt cttccgtctt ggcgaaaata ccgaaatgca aattatgctt
    13441 ttctattttt ctccgacagt cggcgtttcg ggaggcgggc gtggctttca tggacccggt
    13501 cagggacgta tgcaggaaaa ttatttcgtc aaccttggcg ccaaacacaa tttcctcgat
    13561 gggaaaatgt cggctttcgt aagagttagc gacattttca agactcagaa acatggccaa
    13621 acgatttacg gtgataattt caaaactaca tcactgcggc acccggaaag tcaggtcatt
    13681 ttctttggta tttcttataa aatcaacgag ggcatcaaag agcg
//
LOCUS       DAOOMU010000060        13702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4897_length_13702_cov_19.689236, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000060 DAOOMU010000000
VERSION     DAOOMU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13702
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4897_length_13702_cov_19.689236"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1441
                     /locus_tag="PLI55_06780"
     CDS             188..1441
                     /locus_tag="PLI55_06780"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPB00576.1"
                     /translation="MARKISILMLIAVLNAVSLAENLFSNVLNISADSTGNVLLVDKS
                     HQKMFVVRSNAPGALEIVKEYRITTGRRRGDKERSGDLKTPEGIYYIIGRVPDNRLTA
                     RYGPLAYALDYPNYVDRLQKRNGSNIWIHGRDEEIKDFITEGCISLENSHILDLRKYI
                     VVRQTPVIILDNLSDYYVAPENLKAAVSFWTEYINGWTKNWDSGAVAEYCDYYAPNFL
                     DENGRNLKQFKSYKTDLEKRYQWKTVMVDEISVLVSDCEAHLKFRQRYLCPTFYSEGQ
                     KQLILVPIDSNWRIVRETFRPTSPSLTTEEMVTQFLSGWKSAWEQKDIDTYINFYAEE
                     FTSGEYDLSGWKNYKDQVFQSVQNISVKWSALRIQSPQEQVWKVTFRQIYQGDGYRDI
                     GLKTLTLKGFPGNIKIIGEEWVEEK"
     gene            1428..2879
                     /locus_tag="PLI55_06785"
     CDS             1428..2879
                     /locus_tag="PLI55_06785"
                     /inference="COORDINATES: protein motif:HMM:NF028343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14/M99 family metallopeptidase"
                     /protein_id="HPB00577.1"
                     /translation="MKKNKTRKTILLIGLIISWQVASGLTAEIKRPSREHEVYFENTP
                     NELHVYKLYGRFDGNTIFILGGIQGDEPGGYLSADLYPDLVLDRGNLIVIPRANFHSI
                     ILNKRGVNGDMNRRFDRKQSTDIEDQIVAIIKQLMSESDLFLNLHDGSGFYSKTYIDK
                     NRNPYCYGQSIIADADQVAFNGDTLKLEEMAQKVIKGINLRVENPEHKFSFFNTNTLS
                     PDTKYPEQRKSATYYALTTFHIPAFGIESSKNLENVELKIRYHNYAINEFFKLMGVEP
                     EHPAVLFEPPKLIYLLITINNTETKIVENDGFIQVNYGDIIRVKHIESNYNRGLTCDV
                     LGIGNAQDFQKPIPVLKPTSIIVRRDNIIIGSVKVETIGSTFDSIAYIIEVNGIQRKL
                     TDGQTLTVKRGDLIKIIEVLIPGLDSADLQVNLKGFVPPGSYNDGEDRNCLVDTRKLQ
                     WKKYSVDGLGKKYPIVVTYGSDELTRAFIYIQD"
     gene            2994..4907
                     /gene="secD"
                     /locus_tag="PLI55_06790"
     CDS             2994..4907
                     /gene="secD"
                     /locus_tag="PLI55_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPB00578.1"
                     /translation="MFKKNIVRFIIIGVVVVLACYSLYWTVAYHSFSDEKREAMRADG
                     SLDKYERRVIRLGLDLQGGMHIVMEVDLPKLIESLASNKTPQFMALLKEANDEAIANE
                     EDFFDVFRRKINESDLKLVRHFNGRGFKNDEILKSLEEEAKDAVDRAMEVIRNRVDQF
                     GVSEPSIQKSGKYRIIVELAGIQDIGRARELIQSTALLEFILVKDAAVTQQFITAVDN
                     YLKTGKAEEVSPETHEEADSIVALKESKDKAISVDELLGTTSTETGLTAEQADTGLIV
                     DEEIYKERPFSSLLRNIDNQIGVPERNVYAVKKILEDPNVKKLIPFDSKFLWSAKPVR
                     RTLQDGRTENFYILYHVHSEPALLGKYITKASASLGGAGSQSSGQPIVNISMNSEGAR
                     IFAKVTGANIGKQLAVVLDDKVYTAPYIRVKIPDGSAYIEGMSDMEEAKDIAIVLRAG
                     ALPAPMTIVEERTVGPSLGKDSIAVGIRVGIFALLVIMLFMFFYYRRSGLLADMALIL
                     NMVFTLAVMAMLRATLTLPGIAGLVLTIGMAVDANVLIFERIREELERGKTVSAAVST
                     GYSRALSAILDSNITTLLTAIILMQTGTGPVKGFAVTLFWGIASSMFTAIFVTRTFFN
                     WRIEKHAIKTLSI"
     gene            4925..5806
                     /gene="secF"
                     /locus_tag="PLI55_06795"
     CDS             4925..5806
                     /gene="secF"
                     /locus_tag="PLI55_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPB00579.1"
                     /translation="MRRIIKKTDIPFIKYSKTAITISLVIIAIGLISLIFRGLNFGID
                     FTGGTLIQVKFEQPRTIEDIRNDLATINLENSIIQKSGENEYIIRVPQDLSSANLVDV
                     IKSTLGISDQNILRQEQVGPKIGKELQGAALLAIFFSLIVMGIYIAIRFKFQYSIGVI
                     LSLLHDVLITLTVFTIFQLEVSLSTIAAFLTIIGYSLNDTIVVFDRVRENLKLHRNET
                     YERIMDISINETLSRTIITDMTTFFAVLCLLLVPGEVRIFAIAMTIGSIAGTYSTIYI
                     AGPLVVFWSRRFKRSTK"
     gene            5874..7124
                     /locus_tag="PLI55_06800"
     CDS             5874..7124
                     /locus_tag="PLI55_06800"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011612739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPB00580.1"
                     /translation="MPLTAVIGAQWGDEGKGKIVDILSESADLVARFQGGANAGHTVQ
                     IGTEETILHQIPSGILRSQTHCLLGNGMAFDPVGLKNEIDVVQRKGIDVRNRLHISPL
                     AQVVTPLHKILDNYYENISGARAIGTTKRGIGPCYTDKISRNGIRVCDLFNENDLREN
                     ITSRTIFMRKQGVLTESEEDKLADDIEVFLDAVREILPYVDNTIFRIHSYLESGKNVL
                     AEGAQGVLLDIDFGTYPFVTASNTIAGNVATGLGIGPHWIDSIIGVFKCYTTRVGAGP
                     FPTELNDAVGDHLQARGDEFGATTRRRRRCGWFDAALGKYAVKINGFSSLAFTKLDVL
                     DDLAEIKICTHYANGEFPLLDLKNAIPVYLTLPGWQKSTTEARHLDELPFQARQYLQT
                     LEDLLEVKISYISTGKERHQIILV"
     gene            7361..8380
                     /gene="ruvB"
                     /locus_tag="PLI55_06805"
     CDS             7361..8380
                     /gene="ruvB"
                     /locus_tag="PLI55_06805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPB00581.1"
                     /translation="MHSKMIDPQKLAEDYEYEKTLRPSAFEEFVGQKNVVENLKLYIS
                     AAQERGEALDHVLLFGPPGLGKTTLAYIVAKELGVNIRTSSGPVVERAGDLAGMLTNL
                     AARDVFFIDEIHRLNNVVEEYLYSAMEDYHIDIVIDKGPNARSIQLNLEQFTLIGATT
                     RLGHLTSPLRDRFGVILRMDYYNPDELFQILMRTAQILNIELRPDGAAEIAKRSRGTP
                     RVANRILRRTRDYAQVKAAGVIDKKVAEQSLKMLEIDELGLDNLDRQILLTIIDKFSG
                     GPVGLNNLAVAVGEEAETIEDVYEPYLIQQGLIQRTARGRQATPATYEYFHKNISSSQ
                     QKLFT"
     gene            8392..9426
                     /gene="queA"
                     /locus_tag="PLI55_06810"
     CDS             8392..9426
                     /gene="queA"
                     /locus_tag="PLI55_06810"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPB00582.1"
                     /translation="MRLSDFDYNVPEELIAKYPVSKRDHSRLMVIDRKKQTITHKRFY
                     NIIDYLNPGDLLVINETKVYPARLWAVKDRTEAKVEVFLLRELENSLWEVMVKPARKV
                     RIGNKLTIAKGVQCDVIDNTVSGGRVVRFNNIPKETLYKLIDEVGLSPLPPYIDREPT
                     EDDKETYQTVYAKKRGAVAAPTAGLHFTEELMQKIKDKGVKIFPIVLHIGLGTFRPVT
                     VEDLSRHRMDSEYFEVSAETALAINNARSKSKRIIAVGTSVVRTLETVTVSGFQITPR
                     RGWTDKFIHPPYEFKMVDALITNFHQPKSTTIMQVSAFGGHELIMRAYQEAIEKKYRF
                     FSYGDAMLII"
     gene            9407..10141
                     /gene="ispD"
                     /locus_tag="PLI55_06815"
     CDS             9407..10141
                     /gene="ispD"
                     /locus_tag="PLI55_06815"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPB00583.1"
                     /translation="MQCLLSSLNILSLNTPFISGIVVAAGQGKRFGLGIPKQFLKIKG
                     QPLLAYTIDNLLKIGILSELIIVVSDDWINSKELKACLPLPSSVPIKIIAGGEHRQDS
                     VFNGMQVIDSQAEIVVVHDGVRPFVMPELVRETVRLCSEFDGAILAIPAVDTLKKVED
                     GQIVRTIERSRVWQAQTPQTFRREVLERAYANARQNNIIATDDAMLVELIGGKVAVVK
                     STPDNLKITCQNDLKIAKSILKLREP"
     gene            10143..10622
                     /gene="ispF"
                     /locus_tag="PLI55_06820"
     CDS             10143..10622
                     /gene="ispF"
                     /locus_tag="PLI55_06820"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPB00584.1"
                     /translation="MRYRFGSGIDVHRFCSGRPLILGGVTIPFDKGLLGHSDADVLTH
                     AIIDALLGASALGDIGTHFPDTDAQYKDISSLILLEKTREMVVSVGFRVSQIDATVIT
                     QAPRLLPFIPEIRHSLADCLQIPVGDISVKAKTTEGLGFIGSGEGIAALATVTLITL"
     gene            10619..11242
                     /locus_tag="PLI55_06825"
     CDS             10619..11242
                     /locus_tag="PLI55_06825"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPB00585.1"
                     /translation="MNALAEFLTTGSGWIVYVVLFILIFLENVIPFIPGDTVLAFSSY
                     LAGIGILNSFISYPLTVIAAVVGFIFTYVIAYFWGRAFIDRKNFPAFPPRKMARIDRL
                     FHKYGYMGLGIGRFLPGTRLLMAFMAGFTRLRMVPALACISFGIIGWNMLIYLLGYLV
                     GGNRQAIAAFISQYNHIASLIVLALIVILIAWRVNQKIKKLGEKTSD"
     assembly_gap    11289..11388
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11390..12889
                     /gene="gltX"
                     /locus_tag="PLI55_06830"
     CDS             11390..12889
                     /gene="gltX"
                     /locus_tag="PLI55_06830"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPB00586.1"
                     /translation="MIRTRFAPSPTGFLHVGGLRTALYNFLFAKKYNGRFILRIEDTD
                     QRRKVAGAVENLLATLEWAGVKPDEGPQIGGDFGPYVQSERLQLYRDYAQLLLEKGHA
                     YYCFCSAERLKKLKEWQIAQKIPPRYDNHCRDLSVEEIQQHLSSGEEYVIRMKIPAAG
                     AIEVDDLIRGKVTFPVSQIDDQVLLKSDGYPTYHLANVVDDHLMQISHVIRGEEWLPS
                     TPKHVLLYRYFGWELPQFAHLSLLLNPDKSKLSKRQGDVAVEDYRRKGYLPEALINFL
                     ALLGWNPGDDREIFSLDELIREFSLERVSHSGAIFNIEKLNWMNGIYIRNLSLERLWE
                     YAKPYLEECLHQPCVDERAHWDEARLKAVLNSVKDGLSNFSEIPQKTAFYFASSISYE
                     APEIQDYLTTPESITVLKTLIPLIDSVGINQTDGKKPDFNSDSFIAAVRNVQKATGIK
                     GKPLWMTIRVALTGTTQGPEIHQIASIIGRETCIKRLEAALNYAESHLN"
     gene            12867..>13702
                     /gene="ispE"
                     /locus_tag="PLI55_06835"
     CDS             12867..>13702
                     /gene="ispE"
                     /locus_tag="PLI55_06835"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPB00587.1"
                     /translation="MPKATSIKIKSNAKINLTLRITGRRPDGYHNLSTIFQEIDLHDV
                     LTFTPAKTFEFATNHLALSNKTDNLCAKAYRRMEQIAPHSQPWRITLEKNIPIGAGLG
                     GGSSNAATVLKFLNKAWQINLSDDNLARIGCAIGSDVPFYIKGKTQGARGIGEVLEPL
                     ILMEDFVILLVLPEINISTAWAYRQFNPKNAKPAYNFAELISGGRVDWVKFENQFEEI
                     VFPVYPQLAKLKEQILNTGALYAGLSGSGAALFGIFRNRTYALKAARQLPEWRTIITK
                     PV"
BASE COUNT         3876 a         2785 c         3078 g         3863 t
ORIGIN      
        1 ggtattaagt taagcctcgt tgctatgatt ttgcctcagt tttaatctta acctcgacct
       61 tagtctcagc ctcaacttat cttaccaacg aaaattgcct attttaaaaa acgattttat
      121 tatctaattt tggcgcactt taattatgaa tatcagtgat gtaaacttaa aattcagaaa
      181 tacaaatatg gcgcgaaaaa tttcgatctt gatgttaata gctgttttaa atgccgtaag
      241 tctggctgaa aatctattct cgaacgtgct caatatttca gccgactcaa ccggtaatgt
      301 cttactcgtt gataagtcac accaaaaaat gttcgttgta agaagtaacg cgcccggcgc
      361 tctggaaatt gtaaaagagt accgaattac aaccggaagg cggaggggtg ataaggagcg
      421 ttcaggcgat ctaaagacac cggaaggtat ttattatatc attggtcgag taccggacaa
      481 tcgcttaacc gctagatatg ggccgctggc ttatgcgctg gattatccga attatgttga
      541 tcgtttacag aaacgtaatg gatcgaatat ttggatacat ggacgcgacg aagagatcaa
      601 agattttatt accgaaggat gtatttctct tgaaaatagt catatattag atctccgaaa
      661 atacatcgta gtccgtcaga ccccggtaat cattttggat aatttaagcg attattatgt
      721 agctccggag aatttaaaag ccgctgtaag tttctggaca gagtacatca atggttggac
      781 gaaaaattgg gacagcggcg cagtggcgga atattgtgac tattatgccc ctaacttcct
      841 tgatgaaaat ggtcgtaatc tgaaacaatt taagtcctat aaaactgacc tggaaaaaag
      901 ataccagtgg aaaactgtaa tggttgacga aatcagtgta ctagtctctg attgtgaagc
      961 gcatttgaaa tttcgccagc gctatctttg cccaacattt tatagtgaag gccagaagca
     1021 gttaatttta gtgccgattg attcgaattg gagaattgtg cgggaaactt ttcgtccgac
     1081 cagtccaagc ctgacaaccg aagaaatggt tacccaattt ttatccggct ggaagagtgc
     1141 ttgggagcaa aaggatattg atacttatat aaatttttat gcagaagagt tcaccagcgg
     1201 cgagtatgat ctatccggct ggaaaaatta caaagaccag gttttccaat cagtgcaaaa
     1261 tatcagtgtg aaatggagtg ctttgagaat tcaatcgcct caagagcaag tctggaaagt
     1321 aactttcaga cagatttatc agggagatgg ttatcgggat attggcttaa agacgctaac
     1381 acttaaaggt tttccgggaa atatcaagat tattggggaa gagtgggttg aagaaaaata
     1441 aaacccgaaa aacgattctg ttaattggac tgattatttc ctggcaagtc gcgtctggct
     1501 taactgccga aatcaagcgt ccatcgcgag aacatgaggt atattttgag aatacgccca
     1561 acgaactgca tgtctataaa ctttacggac gcttcgatgg caacactatt tttatcctgg
     1621 ggggtattca gggcgatgaa cctggtggct acttatcagc tgacctttac ccagatctag
     1681 tgcttgatag gggtaatctg attgtcatcc cgcgcgccaa cttccattca ataattctta
     1741 ataagcgggg cgttaatggt gatatgaacc gacgcttcga ccgtaaacaa tccacagata
     1801 ttgaagatca aatagtagca attattaagc agttaatgtc cgaaagcgat ctttttctca
     1861 atttgcacga tggtagtggt ttttacagta aaacttatat tgacaagaac cgcaatccgt
     1921 actgctatgg tcagtcaatt atcgccgatg ctgaccaggt agcattcaat ggtgatactc
     1981 tgaaattaga ggaaatggct caaaaagtaa taaaaggcat taacttacga gttgagaatc
     2041 ccgaacacaa atttagtttt ttcaatacta ataccttaag tcctgatact aaatacccgg
     2101 agcagaggaa gtcggcgacc tattatgcgc taacaacttt tcacattcct gccttcggca
     2161 ttgaatcatc gaaaaatctc gaaaatgtcg aactaaaaat tcgttatcat aattacgcga
     2221 ttaatgaatt ttttaagctg atgggagttg aaccggaaca cccagccgtc ctatttgagc
     2281 cacctaaact gatttacctc cttatcacta ttaataatac tgagacgaaa atcgtcgaaa
     2341 acgatggttt tattcaagtc aattatggtg atattatacg tgttaagcat atcgaatcga
     2401 attataatcg tggtcttacc tgcgatgtgc tcggcatcgg gaacgctcaa gatttccaaa
     2461 aaccgattcc cgttttaaag ccgacctcca tcatcgtcag acgtgataat attataattg
     2521 gcagtgttaa agtcgagaca atcggttcga cgtttgacag cattgcctat ataattgaag
     2581 ttaatggaat tcagcgtaag ctaaccgatg gtcaaacgct aactgtcaaa cgaggagatt
     2641 taattaaaat cattgaagtt ttaattcccg gattagacag tgccgatttg caggttaatc
     2701 taaagggctt tgtgccgccg ggtagttata acgatggaga agatcgcaat tgtctggtag
     2761 atacccgtaa attgcaatgg aagaaatatt cagtagatgg tttaggaaaa aaatatccga
     2821 ttgtcgtaac ctatggctct gacgagttga cacgagcctt tatttatatt caggattgaa
     2881 atttggattt atttgaccag gctggctttt tgtttgacat taaacgactc ttgaagttaa
     2941 attcacatgc tttttttaaa ctaaatataa gatatacttt gaggtgattc tgaatgttta
     3001 aaaagaatat tgtccgattt atcattatcg gtgtagtagt tgttctagcg tgctattcat
     3061 tatattggac agtcgcttat cattcctttt ctgacgagaa acgggaagcg atgcgggcgg
     3121 atggttcgct ggataaatac gaacgccggg tcattcggct aggccttgat ttgcagggcg
     3181 gtatgcatat tgtgatggaa gttgatctgc caaagttgat tgaatctctg gcatccaata
     3241 agacgcccca gtttatggct cttctaaagg aagctaatga cgaagccatc gctaacgaag
     3301 aggatttttt cgatgtattt cgtcgaaaaa tcaatgaatc ggacctaaag ttagtgcgtc
     3361 attttaatgg acgcggattc aagaacgacg agattctgaa gagtctggaa gaagaagcca
     3421 aagatgccgt tgaccgcgct atggaagtga ttcgtaatcg cgttgatcaa tttggcgtat
     3481 ctgaaccttc gatccagaaa tctggcaaat accggattat tgtagaacta gctggtattc
     3541 aagatattgg tcgggctaga gaattgattc aaagtacggc tttactggaa tttattctgg
     3601 taaaagatgc ggcagttacc cagcaattca taacggctgt agataattat ttaaaaacgg
     3661 gcaaggcaga agaggtctcg cctgaaactc acgaagaagc cgattcaatc gtagcattga
     3721 aagaatctaa ggacaaggcg atcagcgttg atgaactact gggaaccact tctaccgaaa
     3781 ccggacttac cgccgaacag gctgataccg gcttgatcgt tgatgaggaa atttacaaag
     3841 aacgcccgtt ttcttcttta cttagaaata ttgataacca gattggagtt ccggaacgca
     3901 atgtttatgc tgtcaagaaa attcttgaag accccaatgt gaaaaagttg attccttttg
     3961 attctaaatt tctctggtca gccaaacctg tacgtcgtac tcttcaggat ggtcgtactg
     4021 aaaattttta tattttatac cacgtccata gcgaaccggc tttactaggc aaatacataa
     4081 ccaaagcttc tgcttcgctg ggcggtgctg gttcgcaaag cagtggccag ccgatcgtga
     4141 atataagtat gaatagcgag ggcgcccgca tatttgcaaa agttaccggc gcgaatatcg
     4201 gcaaacagct ggcggtagtc ctcgatgata aagtttatac tgcgccttat ataagagtta
     4261 agataccgga cgggtcggct tatatcgaag gtatgtccga tatggaggag gctaaagata
     4321 tcgctattgt gcttcgcgct ggagcattac cagcccctat gactattgtc gaggagagaa
     4381 ccgtcggacc gtcgctcggt aaagattcta tcgccgtggg tatccgggtt ggtatttttg
     4441 ctctgttggt tataatgctg tttatgtttt tttattatcg gcgttctgga ctattagcgg
     4501 atatggcttt gatcctgaac atggttttca cactggctgt tatggcaatg ctacgagcga
     4561 ctctaactct gcccggtatc gctggtctgg tgttgacaat tggtatggca gtggacgcca
     4621 atgtattgat ttttgaacgt attcgcgagg aactggaacg cgggaaaact gtaagtgcgg
     4681 cggtcagtac cggttactcc cgggcattgt ccgccattct ggattcaaat attacaactc
     4741 tgctaacggc tataatattg atgcagaccg gaacaggccc tgtcaaagga tttgccgtaa
     4801 cgcttttctg gggcatcgca tccagtatgt tcacggctat attcgtgacc cgcaccttct
     4861 ttaactggcg aatcgaaaaa catgctatta aaactcttag catttaagat caggataaat
     4921 tgatatgaga agaattatta agaaaactga tattcctttt attaaatata gcaagaccgc
     4981 tataacgatt tcgctggtaa taatcgctat cggccttata tcgttaattt ttcgcggatt
     5041 gaactttggg attgatttta cgggcggcac tctaatccag gtcaaatttg aacaacctcg
     5101 aacaatcgag gacattcgca atgacctggc aactatcaac ctcgaaaaca gtattatcca
     5161 gaaatctggc gaaaatgaat atatcatccg agtaccgcaa gatttatcca gtgctaatct
     5221 ggtagatgtt atcaaatcta ctcttggaat tagcgaccag aacatcttac gccaggaaca
     5281 agtaggtcca aagatcggga aagaactgca gggagcggct ctactggcta tctttttctc
     5341 tcttatcgtt atgggtattt acattgctat ccgatttaaa tttcaatatt ctatcggtgt
     5401 gattctctcg ttgctccacg acgttttaat taccctaacg gtttttacca tttttcagct
     5461 ggaagtatcc ctatcaacaa tcgcggcttt tctgacgatc atcggctact cactaaatga
     5521 tacaatcgtc gtatttgacc gcgtgcgcga aaacttgaaa ttacatcgta acgaaaccta
     5581 tgagagaatc atggacatca gtatcaacga gactcttagt cgaacgatta ttaccgatat
     5641 gacgactttt ttcgcggtct tatgtctact tttagtccca ggtgaagtgc gcatctttgc
     5701 gatcgccatg acaattggca gtattgccgg gacatattcc acgatttata ttgcaggtcc
     5761 gttagtggtt ttctggtcgc gtaggttcaa acgctccact aaatgattca tataaaacaa
     5821 tcaatgaaaa atgctccctt tctcgggagc attttatttt ttaggagaat aaaatgccgt
     5881 taactgccgt gattggtgcc caatggggcg atgaaggcaa aggtaaaatc gttgacattc
     5941 tgagcgagtc agccgatttg gtagcgcgct tccagggcgg cgctaacgcc ggtcatacag
     6001 ttcagattgg cacagaagaa accattctgc atcaaattcc cagcgggata ttgcgatcgc
     6061 aaacgcattg cctccttggc aacggaatgg ctttcgatcc ggttggtctc aagaatgaaa
     6121 tagatgtcgt ccagcgaaaa gggattgacg tccgtaatcg tctgcatatt tcgccactgg
     6181 cgcaggttgt tacgccacta cataaaatct tggataacta ttatgaaaat ataagcggcg
     6241 cccgagcgat tggcaccacc aaacgcggaa tcggcccatg ttataccgat aaaatcagcc
     6301 gcaatggtat tcgagtatgc gatttattca atgaaaatga cctaagagaa aacattacct
     6361 ctcgaaccat ttttatgcga aagcagggag ttttgacgga atcagaagag gataaattgg
     6421 cagatgatat tgaagtattt ttggatgcgg tgcgcgagat acttccctat gtggacaata
     6481 cgattttccg aattcactcc tatttagaaa gcggaaaaaa cgttttggct gaaggagctc
     6541 agggagtact gttagatatt gactttggca cttacccctt tgtaacggcg tcgaatacga
     6601 ttgccggtaa tgttgctacc gggttgggta tcggaccaca ctggatagat tctataattg
     6661 gagttttcaa atgttatact acccgggtag gtgcaggtcc ttttcccacc gaactgaatg
     6721 acgcagtcgg agaccattta caggcgcgtg gtgatgaatt tggtgccaca actcgccgtc
     6781 gtcgccggtg cgggtggttt gatgccgccc tcggtaaata tgcggtcaag atcaatggat
     6841 tttcatctct ggcattcact aaactggatg tgctggatga tctggcagag ataaaaattt
     6901 gtactcatta cgcaaatggc gaatttccat tattagattt aaagaatgct attccagttt
     6961 acctgactct tcccggctgg caaaaatcca cgactgaagc ccgccattta gacgagttgc
     7021 cttttcaagc tcgccaatac ctccagactc tggaagatct acttgaggtt aaaatttcct
     7081 acatctctac gggcaaagaa cgtcatcaga ttattttagt ataaacaatc cagatcccta
     7141 attattcgtc ggtaaatcta taatacagca ttgctttacg gcttattttt aagtaaatta
     7201 tgccaaattt tgtggtaatg tattgctaaa gaatataaag aattttaaat cactactgga
     7261 atttatttta cttatttcaa cacgaattaa cctgcaactg caatattgtt ttaaaccgac
     7321 aatattaaaa tatcaataaa acgaaaatca ggattttcgg atgcattcta aaatgattga
     7381 tccccaaaag ctggccgaag attacgaata tgaaaaaact ttacgccctt cggcatttga
     7441 agaattcgtc gggcagaaaa atgtggtgga aaatcttaaa ctatacattt cagcggcgca
     7501 ggaacgcggt gaagctttgg atcatgtcct tttattcggg ccgcccggtt taggcaaaac
     7561 tactctggcc tacatagtgg ctaaagagct aggagtaaac attcgcacca gttccggacc
     7621 ggttgtagaa cgagctggcg atctggctgg tatgctaact aatttggcgg caagagatgt
     7681 atttttcata gatgaaattc atcgcctgaa caacgtggtt gaggaatatc tttattctgc
     7741 catggaagat taccatattg atattgtcat tgataaagga ccgaatgctc gcagtatcca
     7801 gcttaacctc gagcaattta cgctaattgg agccaccacc cggctgggac atttgacctc
     7861 gcccttgaga gaccgctttg gagtaatttt gcgaatggat tattataatc cggacgaact
     7921 attccagatt ctgatgcgta ccgcccaaat tttaaacatt gaactgcgcc cggacggcgc
     7981 cgccgaaatt gccaagcgta gccgcggaac accgcgcgtc gctaatcgga ttttacgccg
     8041 cactcgcgat tatgcccagg tcaaagccgc tggcgtaatt gataaaaagg tcgccgaaca
     8101 atccctgaaa atgctggaaa ttgatgaatt aggactggat aatttggacc gccagattct
     8161 tttaactatc attgataaat tttccggcgg accggtcggg ttgaataatc ttgcggtcgc
     8221 agtcggtgaa gaagctgaaa ccatcgagga tgtttatgaa ccttacctga ttcagcaagg
     8281 attaattcag cggaccgctc gcggacgcca ggcaacacca gcaacctatg aatatttcca
     8341 taaaaatata agtagctctc agcaaaagct gtttacgtag gaggaaaata aatgcgactt
     8401 tccgattttg attataatgt gccggaggaa ttgattgcta aatatcctgt aagtaagcgc
     8461 gatcattccc gattaatggt cattgaccgt aaaaaacaga ctatcactca caagagattt
     8521 tacaatatta ttgattatct caatcccggt gatttgctgg ttatcaatga aaccaaagtc
     8581 tatcccgctc ggctttgggc ggttaaagac cgtacagaag ctaaagttga agtatttctt
     8641 ttgcgcgaat tggaaaatag tctttgggaa gttatggtca aaccagcccg caaagtccgg
     8701 attggcaata aattgaccat tgctaaaggt gttcagtgcg atgttattga caataccgtt
     8761 tcaggcggtc gggtcgtgcg ttttaataac attcccaagg aaaccctcta taaattgatt
     8821 gatgaggttg ggctatcgcc tctgccgccc tatattgacc gcgagccgac tgaagatgat
     8881 aaggaaacct accagactgt ctatgccaag aaacgaggcg cagttgccgc tcccaccgcc
     8941 ggtttgcatt ttactgaaga attaatgcag aaaattaaag acaagggcgt taaaatcttt
     9001 cccattgttc tgcacattgg tttaggtact ttccgcccgg tgaccgtcga ggatttgtct
     9061 cgtcatcgga tggattccga atattttgag gtttcagctg aaacggctct cgctataaat
     9121 aatgcccgct ccaaaagtaa aaggatcata gcggtaggta cttccgtcgt gcggacgctt
     9181 gaaacggtca cagtttcagg tttccagatt acaccgcggc gtggttggac cgataaattt
     9241 attcatccac cgtacgagtt caagatggtt gatgctctga ttaccaattt tcatcaacct
     9301 aaatccacca cgattatgca ggtttcggct ttcggtggcc atgagctgat tatgagggca
     9361 tatcaagaag ccatcgagaa gaaatatcgc ttcttcagct atggcgatgc aatgcttatt
     9421 atctagttta aatatcttgt cccttaacac tccattcatt agtggaattg tggtagccgc
     9481 cgggcaaggg aaacgctttg gtttagggat ccccaagcaa tttttaaaaa taaaaggtca
     9541 gccgctccta gcttatacca ttgataatct gttaaaaatc ggtatcctta gtgaactaat
     9601 tattgtcgtt agtgatgatt ggatcaattc caaagaatta aaagcctgtt tgccattacc
     9661 gagtagcgtc ccgatcaaaa tcattgccgg tggtgaacat cgccaggatt cggtctttaa
     9721 cggtatgcag gtcattgatt cacaagcgga gattgtagtt gttcacgacg gagtccgtcc
     9781 gtttgtaatg ccagaactcg ttcgagaaac agttcgcctt tgctcagagt ttgacggggc
     9841 aattctggca atcccggctg tggatacttt gaagaaagtt gaggatggcc agattgtaag
     9901 aacaatagaa cgtagtcgag tctggcaggc tcaaacaccg caaaccttcc gtcgggaagt
     9961 gcttgagcgt gcctacgcca acgctcggca aaataatatt atagccaccg atgatgccat
    10021 gttagtggaa ttaatcggtg gaaaagtagc ggttgtcaaa agcacgcctg ataatttaaa
    10081 aattacttgt caaaatgact tgaaaattgc aaaatcaata ctgaaactga gagagccttg
    10141 aaatgagata tcgttttggc agtggaatag atgttcatcg cttttgtagc ggtcgccctt
    10201 tgattttggg cggagtaacc atcccgtttg ataagggctt actgggacat tccgacgcag
    10261 atgtattgac tcacgccatc attgatgctc tattgggagc ttctgcgtta ggcgatattg
    10321 gcactcattt ccccgatact gatgcgcaat ataaagacat ttccagtttg attcttctgg
    10381 aaaaaacccg tgaaatggtc gtctccgtcg gctttcgcgt cagccagatt gatgccaccg
    10441 tcatcactca ggctccgcgg ctattgccgt ttattccgga aattcgccac agtcttgctg
    10501 actgcttaca aataccggtc ggtgacattt ctgtgaaagc caagacgacc gaaggactgg
    10561 gtttcattgg cagtggcgaa gggatagcgg ctttggcgac ggtaactctt ataactttat
    10621 gaatgcttta gctgaatttt taacgacggg atcaggctgg atagtctatg tcgttttatt
    10681 tattttgata tttttggaaa atgtcatacc gttcattccg ggtgatactg tgctggcttt
    10741 ctcctcatat ttggcaggta ttgggatatt gaattcattt atttcttatc cactaactgt
    10801 catagcggcg gtcgtcggtt ttattttcac ttatgtaatt gcttattttt ggggcagagc
    10861 gtttattgac cgcaaaaatt ttcccgcatt tccgccccgc aaaatggcaa gaattgaccg
    10921 gctttttcat aagtatggct atatggggtt gggcataggg cgatttttgc ctgggacgcg
    10981 gcttttgatg gcttttatgg ccggttttac gcgtttgaga atggttccag cgttagcgtg
    11041 tatctcattc ggtataatcg gctggaatat gctaatttac ctattggggt accttgtagg
    11101 aggaaatcgt caggcaattg ccgcttttat atcgcaatat aatcacatcg ccagcttgat
    11161 cgttttagca ttaatagtaa tactgattgc ctggcgtgtg aatcagaaaa taaaaaaatt
    11221 aggtgaaaag actagtgatt agaactcgct tcgcacccag cccaactggc tttttacacg
    11281 ttggcggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag tgattagaac
    11401 tcgcttcgca cccagcccaa ctggcttttt acacgttggc ggtttacgta ctgcactcta
    11461 taacttctta tttgccaaga agtataacgg gcgttttatc ctgcgcattg aagataccga
    11521 tcaacgtcgc aaagtcgcgg gagccgtcga aaatctgctg gcaacccttg aatgggctgg
    11581 agtcaagccg gacgaaggac cgcaaatcgg cggcgacttt gggccatatg tgcagtcaga
    11641 acgacttcag ctttatcgtg attatgctca attattgctg gaaaaggggc atgcttatta
    11701 ctgcttctgt tcagcagaac gactaaaaaa attgaaagaa tggcaaattg cccagaaaat
    11761 accaccgcgt tacgataatc attgccgcga tttgtcagta gaagaaatcc aacaacatct
    11821 atcttctggc gaagagtacg tcatccggat gaaaattcca gccgctggtg ctattgaagt
    11881 tgacgatctc atccggggca aagtcacttt tccggttagt cagattgatg accaggttct
    11941 gcttaaatca gatggttatc caacttacca tctagcaaat gttgtagatg atcacttaat
    12001 gcaaatcagc catgtcattc gcggcgaaga atggttgccg agcacaccca agcatgtttt
    12061 attgtatcgc tatttcggtt gggaattgcc gcaatttgcg catctttctt tattgctaaa
    12121 tccggacaaa agtaaacttt cgaagcggca aggtgatgtt gcggttgagg attaccggcg
    12181 caaaggctac ctgccggaag ctctgataaa tttcctggct ttattaggat ggaacccggg
    12241 cgacgaccgt gaaattttta gtctcgacga attaattcgt gaattttcct tggaacgcgt
    12301 cagtcatagc ggggcgattt tcaacatcga aaagctcaat tggatgaatg gaatttacat
    12361 ccgtaatttg tcgttggaac gtctctggga atacgccaaa ccatatttag aggaatgtct
    12421 ccaccagcct tgcgtggatg agcgggctca ttgggacgaa gcccgattaa aggcagtgtt
    12481 aaactctgtt aaggacgggt tgtccaactt ttccgaaatt cctcagaaaa cggcttttta
    12541 ttttgcctca tcaatttctt atgaggcgcc tgaaattcag gattatttaa ccacaccgga
    12601 atccataaca gtcctgaaaa ctctgatacc tttgatagat tctgttggaa taaatcagac
    12661 ggacgggaag aagccagatt tcaacagcga cagctttata gcggctgtca ggaatgttca
    12721 gaaagcaact ggtattaaag ggaaaccgct ctggatgacc attagagtcg ccctaaccgg
    12781 caccacgcaa ggcccagaaa ttcaccaaat tgccagtata attggtcgtg aaacctgtat
    12841 caagcgcctc gaagcggctt tgaattatgc cgaaagccac ctcaattaaa atcaagtcta
    12901 acgccaaaat taatcttacc ttgcgcatca ccgggcgtcg tccggatggc tatcacaacc
    12961 ttagtaccat ttttcaggaa attgatttgc acgatgtcct taccttcaca ccggctaaaa
    13021 cctttgaatt cgccacaaat catttagcac tttctaataa aacggacaat ttgtgtgcca
    13081 aagcataccg aagaatggaa cagattgctc cacatagtca accctggcgg attactttag
    13141 aaaaaaatat cccaatcggc gccgggctgg gcggaggcag ttccaatgcg gcaactgtct
    13201 tgaaattttt gaataaagcc tggcaaatca acctgagcga tgacaacctg gctcgtattg
    13261 gatgtgcgat tggcagtgat gtgccgtttt acatcaaagg aaaaactcaa ggcgccagag
    13321 gaattggcga agtactcgaa ccgctaattc taatggaaga ttttgtcatt ttgttggttc
    13381 tgccggaaat aaatatttcg acagcttggg cgtaccggca gttcaatccc aaaaatgcca
    13441 aaccggccta caactttgcc gaacttatca gtggtgggcg ggttgattgg gtaaaattcg
    13501 aaaaccaatt cgaagaaatc gtttttccgg tatatcctca gttagcaaaa ctgaaggagc
    13561 aaattctaaa tacaggtgcc ctttacgctg ggttatcggg aagcggcgcg gctctgtttg
    13621 gaatttttag aaatcgtact tacgctttaa aagccgcccg gcaacttccg gaatggcgta
    13681 caattatcac caaaccggtc aa
//
LOCUS       DAOOMU010000061        13535 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_4985_length_13535_cov_15.113056, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000061 DAOOMU010000000
VERSION     DAOOMU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13535
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4985_length_13535_cov_15.113056"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1995
                     /locus_tag="PLI55_06840"
     CDS             190..1995
                     /locus_tag="PLI55_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPB00588.1"
                     /translation="MTNDKQSTVVSGSRLGQILVEKGIISSEILEQALKIQNEGDASS
                     SKRLEDILVSNFKIDHDLVFGTLAELYAFRTYRVNPEKLDPAQIKHTKELLSKFPDEL
                     KNQLLYYKVLPYQVINGSRNKLLVLASNPTEKVVEKIPLLTEFKKYEVVYTPLKIVEE
                     LIDLISPRENEFLDLLREAVVEPEIGEEKQVTELDEYSLDEEINKSLLVNLYEGCLIE
                     AVRRRASDIHIVPYEKNSVDIYFRIDGRLQLWYRQDNVSPEAMAAVVKDRTIGVDRFE
                     RDTAQDGFFQRNVDDYLIRYRVSVLPIISEEYERRFESIVIRIIDDRNVIADLRQLGF
                     LPQAEQDFLKAIGKSKGIVILTGPTGSGKSTTLLAALHHVMDASKNILTIEDPVEYAI
                     KGARQLKIGRKLSFEEAIRTILRHDPDIVMVGEIRDKITADVAVKLANTGHLTFTTLH
                     TNDAPSAISRLYKMGVEPFLLAYAINIIVAQRLVRKLCPFCRVPISSEKYPAALELGL
                     TEEDLKSGKIFDAGTGCKKCNGGYKGRISLAEALYFSPEVRKSILNSGAEVDEAEIRE
                     IAEGQGMLSILDSGLEKIREGVTTVSEVSYAASEG"
     gene            1998..2417
                     /locus_tag="PLI55_06845"
     CDS             1998..2417
                     /locus_tag="PLI55_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00589.1"
                     /translation="MGYPQMFALIGSIALFTSVVLTMNTSINNQRQIVYQSIYMNQAV
                     KLASKVFDKITVETKTDLIDFDDIAITYSGTDSLRIWGNDVWYIYNVSSHYCDSSGVD
                     AAGQTDFQRVDIKIWTNPFVILDTLKFNNIYYNIELV"
     gene            2414..2989
                     /locus_tag="PLI55_06850"
     CDS             2414..2989
                     /locus_tag="PLI55_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00590.1"
                     /translation="MTSLLDAIFATSIGGLFLITIFTTLFNLQASSQDTTFHVTLNNA
                     AEVQYQELDNCLGKVGAGVYSGQILVAEPDSFVFKTKYDLINDIMGSVINTISISLGD
                     STSQGIPIKIMQNGVPLPGSNVPLWLRDLNFSYYDIDENQITTPGDSLSYIRLLKTDL
                     QFAFETSYVGPGERNIITNLTFWKYFINFYL"
     gene            3029..4261
                     /locus_tag="PLI55_06855"
     CDS             3029..4261
                     /locus_tag="PLI55_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00591.1"
                     /translation="MGRAILIGVIILTVTFTAITVGVQKRVSNIPDQITESIDFMNSK
                     NLRDFALTYSLKKAYELDDDEDWHLAGFLSERLDVDFSDKLSDFFGGTIDSIRYTALN
                     PAGDSIRISSFITQHIGKGVKAFETEAMVKFEIEEHPKSEIFGFDFDESDPNLVFDGS
                     NNNNNGILGNGVTNYKPTRTVGKNGSALDFDGDNDYVYVGENLIEPDGDELTVGTWVN
                     FSNTNTDWGILAAEKKSNSDHNPIWSVRARKTAGLKIFGFEIFPATIKVAFDIYNGVG
                     SYGYTEVDISKTEHEIDMNSWHYVVATYREVGDVTALISISIADLTYDNKRTKSTTKW
                     QGRTDESYVTVGGDIYQSTYTGFLANLLNEVINRLNCTDTKLDEVKILNEALDDDDIQ
                     DLVSLGGIKDLRLAYWIQ"
     gene            4654..4932
                     /locus_tag="PLI55_06860"
     CDS             4654..4932
                     /locus_tag="PLI55_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00592.1"
                     /translation="MSDQEELIRKYEEVVEVAASIASIDHDINNQLMVFALVERRLQN
                     AAQQYNDEKLLRSSQQVSEALDNIKRILSRLQNLKKLDLIKEKREKRE"
     gene            4939..5796
                     /locus_tag="PLI55_06865"
     CDS             4939..5796
                     /locus_tag="PLI55_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB00593.1"
                     /translation="MQSHILVVDDDKTIRMLCKEILEEAGYKVTLAADGEEALEKMDY
                     DDFDLYLVDMVMPRMDGLELMKKIKQKQPLAVVMILTGFSSIEGAIKAVHAGAFQYLS
                     KPINIDELLNAVKEGIKYSQDLYGPLLKIFEPGTELVEKGEMAILSGFSEEDKQDFMI
                     LSSIYNFEPGENIRIADKMASSIILIESGEVSVWLSNTSVDYLKKWDTWNEESFVVNS
                     PTSSVLRAETPVTARFFERKKLLEFFAFKGEKLLKKFMINLINCLYIKWRKSIQRIIM
                     LKLVTTESE"
     gene            5892..7115
                     /locus_tag="PLI55_06870"
     CDS             5892..7115
                     /locus_tag="PLI55_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPB00594.1"
                     /translation="MGLSFTPEMEIALPEYLIGTARFKNINKLITFNNAWKKEAYDNL
                     KSLLKKMEEIDASDIDIGGSMLDVKIWYRVYGRKYPDLEAKRYSNDEITAILLSILSD
                     DQKIILFDKKNIDFSLGISFGEKEANRFRGDIYYDNDVLVGNFRRVNRELFPIESLNI
                     PEPVLKRLNLRHEKSGLILITGITGSGKSTTLDSIINLNNEENEGHIVIISDPLEFIH
                     KSKKCIIRHREVGGDVLSFREGTIQALRQDPDIIIVGEMRDAPTIATVLEATDSGHKV
                     FTTLHTSSAVESIHRIIAEFLPEEQERIRLRLADTLSVVISQKLVPDRDNKLTLVKEI
                     LSVTDSVKAAIRNKNIGEIYQMITEGKKYGMVTMEQDLHNLYTKKIISRETALDFSNN
                     RKRIEQLLAYREICV"
     gene            7145..9304
                     /locus_tag="PLI55_06875"
     CDS             7145..9304
                     /locus_tag="PLI55_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00595.1"
                     /translation="MKNYYGIFQDGLDIQIAHLVRDEDLIKIQNLGETTLSEPLYNKK
                     EVNKIPTIELSDTNLEIQNNPDLKEEGFDLPEISEFDEFESNKLTEEITEVNEEGGTS
                     TSELQRFLSNFSLETGKIALNANEEQISYHIFNSSLTFSAIKKKIKTEILSQEELKSR
                     KYAIGCIPNYDGMTLAFLHRGEFDLLNAILQVRPLLSPKRYIFSYIDTNEIALMNLIR
                     ANYNFPENDYVLILYVGSEYKAGIVMRNNTHVKTFKIIGDTSSRSENIRQVIYSKIIL
                     EQDVSDIPPVQHLILTGNMVRDEDIRFFSSKFGETTEVSRFEFKNINIATINRKYSPE
                     NIAKYAIPISLALKAADPKNKAFYNTNLLPPKIIESQKRFKIGWHGFILFIAIFYFTY
                     SATLKNLENNRQIVELQRQNYNIESELRHKNLMFARANELSQQITGKRKSMDKIKTIS
                     GNKSQWHRTIIALSDAFAKNSISWINYLRSDTSGFMVQGTTNHKENIAYITRYFPGCI
                     VSDVKTSEIQSIPVMRFVMNFSYSDQEEWVMKVAKPAPVTPQSGKTAAPPPITPAPKS
                     IESESAPKLAAPAPAVVQSQSPPAQSTPRITPYTVFREAAETYLSGNVQEGYHKLTQF
                     VEKYPNSPFTYSANYLIGECLFLMGEVAQAKDIFEKIANQDGTKTPDALLMLGRCYNA
                     LGDKAKAIDCWGMLITKYPEHKLAKMAEEKIKYVTEH"
     gene            9306..10172
                     /locus_tag="PLI55_06880"
     CDS             9306..10172
                     /locus_tag="PLI55_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00596.1"
                     /translation="MPYKIRNTIALAVILLIVLIGSVISNSSSERKIADLSKKNQELS
                     KRLNMLNSQLENIGEEEELEQEIRELEIEISKFKKAIVKEDNPTITYDYLTQICQKFC
                     PEMVFDFKPAESGEIQGTKYNDYLIAGIVPFNSLYTFIYQIENQPKLYLVELLEIREQ
                     PASEDIIPFAFKLRAFYDENGIEPESIPIREIKTVNIRFNPFYPGIHDPVQIKEEEQY
                     LNPEEAILVGLTPTIAFFKDKNGRTISLTTGEKVAYGYLSKIDWENQAVIFKINRIGV
                     YVEKTIYLQGKG"
     gene            10176..>10445
                     /locus_tag="PLI55_06885"
     CDS             10176..>10445
                     /locus_tag="PLI55_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00597.1"
                     /translation="MKKKVLFICLLLFFSSTNSQVNLIKSPSSDELITISPEVLVADA
                     LKMIEALSQKYEGRKIVNMSTYTGPISIPITQIYWKNALNLIANYN"
     assembly_gap    10446..10545
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10546..11231
                     /locus_tag="PLI55_06890"
     CDS             <10546..11231
                     /locus_tag="PLI55_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00598.1"
                     /translation="EVLSDIISGTVSTTLESGEAIIELNALFKMIEHYNKGTIIARPS
                     VTVISGKQGYLQIGQDFSVRTTDFSGNLVDRFYSAGLILEVTPTIIQDSSKEAIHLVA
                     RVEKSSVVPSDVQVIVNKNQATTEAILYDGEETVVAGLYDTEDIVVRKGVPILKDLPW
                     WVLGLRYIFGYNNHEKNEREIIIILKAELIEPIEVRMAKKESARELIEKYYKPKEHID
                     TLLFKKVQE"
     gene            11294..13369
                     /locus_tag="PLI55_06895"
     CDS             11294..13369
                     /locus_tag="PLI55_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00599.1"
                     /translation="MKNKLKITGLFLFLAISLVVIFDCGKITSPGGIEIPENPRIKTI
                     DDNNIEVSWDYDQSDQDVFFTVARKVGDANWNLEYAQTYDNFLIDEIPTNDSLIYAYK
                     IKTESDTSELASEFSDVVAYFSPKTIPTEIAISVLESDKVEVTWRDNCIGEDGYKIEK
                     KVSGGNWIKDYKTFGRNVTSFTDKTSFGDSVFYRVYAFCGISVSGSAEGFGISTFPPP
                     YNLEYERIAIDKIKLTWQSKTSNVAGFKIDKKVGSADWQVEIATLDPNQTWWIDENAE
                     INEILQYRVYGFSEEYISSYVTTDEIDNTFPAPSNLTLTKIDEDIVKISWQDNSQGES
                     GFYIDRKVGMSDWVNGYASVDSNQTTLLDNISEPCGNFYYRVRAFKNQYFSEPTDEGN
                     INIRLNLVSSLTTEGTATEVFVSDWKAYVANHYNGLIVIDLINPSSPSTDNNLVLPLP
                     DRTLSIYIYKNLAYATNHTGGLNIVDVTTLELIGNCQTSGVPVDVFVTGEPPYAYLCE
                     EEKGVSIASILSSTPHIISNITPPNTSADEKAKKLFVANDYAYVAYGSRGLTIFDVSD
                     VKNPIYVADFPSSSITQDVFIKDNYAFLADGENGLVIVDVSSVEAPSLVSNCPTHGFA
                     YSIYVRGNYAYLADKEKGLIVVNISDLANPFILGSFEMSTTPNSVFVDGSYAFVTDSE
                     GLKIIQIAP"
BASE COUNT         4402 a         2287 c         2869 g         3877 t
ORIGIN      
        1 aaaaaataaa ttaatatgta tattaggact attggtgaat attattaaaa aaggactgta
       61 attaatgaac gataacccaa gtccagcatt aaccgaccaa ctaccaatga ttccagtgaa
      121 atactcgctt tattacgctc gccaatttca caggaaaacc aatgaccaat gactaatgac
      181 taatgaccaa tgactaacga caagcaatca acagtagtaa gtggctcccg actggggcaa
      241 atactggtcg agaaaggcat aatcagctca gaaattctgg aacaagccct taaaatccag
      301 aatgaggggg atgcttctag ttcgaaaaga cttgaagata tactggtctc aaatttcaaa
      361 attgatcatg atttggtgtt tggtacgctg gcagaattat acgctttccg aacttaccga
      421 gtaaatccgg agaagctcga tccggctcaa ataaagcaca ctaaggaatt attatccaaa
      481 tttccggatg agctaaaaaa tcaactgcta tattacaaag ttctgcctta tcaagtaatc
      541 aatggttcca ggaataaatt attagtatta gcttcgaatc cgacggagaa ggtcgttgaa
      601 aaaattccgc tattgaccga atttaaaaaa tatgaagttg tttatactcc tcttaaaata
      661 gttgaggaat tgattgacct aatatcgccg cgagaaaatg aatttttaga ccttttaagg
      721 gaggcggtag tcgagccgga aataggcgaa gagaaacaag ttactgaatt agacgaatat
      781 tctctggatg aggaaatcaa taaaagtctt ctggttaatc tttatgaagg ttgtttgatc
      841 gaagccgtgc ggaggagagc cagtgatatt catatagttc cttatgaaaa gaacagcgtt
      901 gacatttatt ttagaattga tggaagatta cagttatggt ataggcagga taatgtttcg
      961 ccggaggcga tggcggcggt agtcaaagat cgaactattg gggttgatcg ttttgaaaga
     1021 gataccgctc aggatggttt ttttcagagg aatgtagatg actatttaat tcgctatcgg
     1081 gtttcggttt tgcccatcat ttctgaagaa tatgaaaggc gtttcgagag cattgttatc
     1141 cggattatag atgatagaaa tgtgattgcc gacttaaggc agttgggatt tttaccccag
     1201 gctgagcaag attttcttaa agcaataggt aaatctaaag ggatagtaat ccttaccggg
     1261 ccgaccggaa gcggtaagag tacgactctt ctggcggccc tgcatcatgt tatggatgcg
     1321 tcgaaaaaca ttctcaccat cgaagacccg gtagagtatg ctatcaaagg agcaaggcag
     1381 ttaaagattg gtagaaaatt aagttttgaa gaagctatca gaacgattct gcggcacgat
     1441 ccagatattg tgatggtagg tgaaattaga gacaaaatta cggcggacgt agcggttaaa
     1501 cttgccaata ccggtcattt gacttttact accctgcata cgaatgatgc tcccagcgct
     1561 atctcgcgct tatataaaat gggtgtcgaa ccgtttttgc tggcttacgc cattaacatt
     1621 atcgtggctc agagattagt gcgcaagctc tgtccttttt gccgcgtacc tatatcttca
     1681 gaaaagtacc cagccgcgct agaattgggt ttaacagaag aagacctgaa atcgggcaaa
     1741 atctttgacg ccggtaccgg atgtaagaaa tgcaatggcg gctacaaagg tcgaattagt
     1801 ttagccgaag cactctattt cagccctgaa gttcgaaagt caatcttaaa ttcgggagct
     1861 gaagtggacg aagctgaaat tagagaaatt gccgaggggc agggtatgct ctcgatcctt
     1921 gattcaggat tagaaaagat cagagaggga gtaaccactg tttcggaagt ttcttatgcc
     1981 gcatcggagg gttagagatg ggttatcctc aaatgtttgc tctgataggt tcgattgcac
     2041 tttttacgtc ggtggtatta acgatgaata cctcaatcaa caatcaaaga caaattgttt
     2101 atcaatcaat ctatatgaat caggcggtaa aattagccag taaagtcttt gataaaataa
     2161 ctgtcgaaac aaagacggat ttgattgatt ttgacgatat tgccatcact tattccggca
     2221 cggattcact gcgaatctgg ggtaacgacg tctggtatat ttataatgtt agtagccact
     2281 attgtgattc cagcggggtg gatgccgccg gccaaaccga tttccagaga gtagatataa
     2341 aaatctggac taatcctttt gtgatccttg atactctaaa gtttaataat atttattata
     2401 atatagaatt agtatgacca gtttattaga tgcaatattt gcgacaagta tcggcggttt
     2461 atttttgata acgatcttta ccaccctgtt taatttgcag gcatcttcgc aagacaccac
     2521 tttccacgtc acgctaaaca acgcggcaga agttcaatat caggagctcg ataattgcct
     2581 gggaaaagta ggagcagggg tttatagcgg acaaatttta gtcgccgagc cggacagttt
     2641 tgtattcaaa actaagtatg atttaatcaa tgatataatg ggttcggtaa ttaataccat
     2701 tagcatttca ttaggcgata gcaccagcca ggggattccg attaaaataa tgcaaaatgg
     2761 ggtgcctttg cccggctcaa atgttccatt atggctgaga gatttgaatt tttcctacta
     2821 tgatattgat gagaatcaaa ttactactcc gggagattcg ttgagttaca tccgtttatt
     2881 aaaaactgat ctgcaatttg cgtttgaaac cagttatgtt ggccctggcg aaaggaatat
     2941 tataaccaat ttgacttttt ggaaatattt tataaatttt tatttatgat ggaaataaat
     3001 tgtaaaaaac ttatgaggag gtattaaaat gggcagggca attttaattg gagtaattat
     3061 tcttaccgtg accttcaccg cgattacggt aggagtgcaa aagcgagtta gtaatattcc
     3121 tgaccagata accgagagta tagattttat gaattctaaa aatctgcgcg attttgcgct
     3181 gacatattcg ttgaaaaagg catacgaatt ggatgacgat gaagattggc atctggcagg
     3241 atttttaagt gaaagattag acgtagattt cagtgataag ttatctgatt tttttggtgg
     3301 tacaattgat agcattcgtt atactgctct taatccggct ggagatagta ttcggatatc
     3361 gtcttttatt actcagcaca tcgggaaagg agtaaaggct tttgagactg aagcgatggt
     3421 gaaattcgaa atagaggaac atcctaagtc agagatattc ggttttgatt ttgatgaaag
     3481 tgatcctaac ctggtttttg atggctcaaa caataacaat aatggaattt taggcaatgg
     3541 tgttaccaat tataaaccta ccagaaccgt tggcaaaaat ggctcagcgc ttgattttga
     3601 tggtgataat gattatgtgt atgtaggaga aaacttaatt gagccggatg gcgatgaact
     3661 taccgtcggg acatgggtaa atttttccaa tactaatacg gattggggaa ttctggcagc
     3721 tgagaaaaag agtaatagtg accataatcc tatatggagt gtaagagctc gtaaaaccgc
     3781 aggacttaaa atttttggct ttgagatctt ccctgcaact attaaagttg ctttcgatat
     3841 ttataatggc gtaggatcgt atggctatac cgaagttgac attagcaaaa ctgaacatga
     3901 gatagatatg aatagttggc attatgtggt agcaacctat cgtgaagttg gggatgttac
     3961 ggcgcttatt tctatttcaa ttgctgacct gacgtatgat aataagagaa ctaaatcaac
     4021 tacgaaatgg cagggacgca ccgatgagag ctatgttact gttggtggtg atatttatca
     4081 gagtacttat actggttttt tagccaattt actaaatgag gttattaata gattaaattg
     4141 taccgatact aagctggatg aggtcaagat tctgaatgaa gctctggatg atgacgatat
     4201 tcaagatctt gttagtctcg gaggaataaa ggatctgaga ttagcctatt ggattcaatg
     4261 agaactatcg gacactgtcg ttaagagata tgatgttttt tgaaaattaa gtagtagaaa
     4321 gccatctcgg gtgccccaag tggctcttag ttattggtat gccccgtaaa atttgcagta
     4381 tagcgaagca agtatttcac agggggtcat taatcaagac taaaggttac tgtgggattg
     4441 agtcgaaatg agaatggctt gagagcagtt ttgaacgtta aaatgtaagt ttattcgctg
     4501 atgttgtttg atcggaaaca gatgattttg gtcagaagcc aaacagaaat tgacggaggt
     4561 tgtttttcta atttgattct tcactgcttt acactataag gtgagtgttg taattggaat
     4621 aatgatgcaa gaataaaata agggaaaaat tatatgagtg atcaggaaga gttgattcgt
     4681 aagtatgagg aagtggttga agtagccgct tccatcgcga gtattgacca tgacatcaat
     4741 aaccaattga tggtttttgc cctggtagaa cgccgattgc aaaatgcggc gcaacagtat
     4801 aatgacgaga aattattgcg gagcagtcaa caggtttcag aagcacttga taatataaaa
     4861 cgcatcttga gtagattaca aaatctcaaa aaattagatt taattaaaga aaaaagagag
     4921 aagcgagaat agaaaattat gcaaagtcat attcttgtcg ttgacgatga taaaactatt
     4981 agaatgctct gcaaggaaat cctcgaggaa gccggatata aagttacctt agccgctgat
     5041 ggcgaggaag cgctggaaaa aatggactat gatgatttcg atttatatct ggtagatatg
     5101 gttatgccgc ggatggatgg attggagctg atgaagaaga taaagcaaaa acagcccctg
     5161 gcggttgtaa tgattctaac cggattttcc agtatcgaag gggctattaa agccgtccat
     5221 gccggtgcat tccagtattt atcaaaacca atcaatattg atgaactgct taatgcggtc
     5281 aaggagggta taaaatactc gcaggattta tatgggcctc tcctgaaaat attcgaaccg
     5341 gggaccgagc tagtagaaaa aggtgaaatg gctattttaa gtggattctc cgaggaggac
     5401 aagcaagatt ttatgatact cagctcaatt tacaattttg aacctgggga gaatataaga
     5461 atcgccgata aaatggctag ttcaataatt ttgattgaaa gtggcgaagt ttcagtttgg
     5521 ttaagtaata cctcagtaga ttatctgaag aaatgggata cctggaacga agagagtttc
     5581 gtcgtcaata gtcctacctc cagtgtactg agagcggaaa ccccggtaac agcccgcttt
     5641 tttgagcgta aaaaattact cgaattcttt gctttcaagg gagaaaagtt gctcaaaaaa
     5701 tttatgatta acctaatcaa ttgcttatat atcaagtgga ggaaatcaat tcagagaatt
     5761 attatgctga agctggtaac cacagagagt gaataaaggt tgaatttact catctaccat
     5821 tttgatataa tgtatgaact taaatagagg atataaaaat tgaccgattt acgtaagtct
     5881 atgaggtaaa aatgggatta tcttttacac ctgaaatgga aatagcctta ccggagtatc
     5941 taattggtac agccagattt aaaaatatta ataagttaat tacattcaat aacgcctgga
     6001 aaaaagaagc ttatgataat ttaaaaagct tattaaaaaa gatggaagag atagacgctt
     6061 ccgatataga cattggcggt tctatgttgg atgtgaaaat ctggtataga gtatatggca
     6121 ggaaatatcc cgatttggaa gctaagcgtt attccaatga tgagatcacg gcgattttgc
     6181 tcagtatatt gtccgatgac cagaaaatta ttcttttcga taaaaagaat attgattttt
     6241 cactaggaat aagttttggt gaaaaagaag ccaaccgctt tagaggtgac atctattacg
     6301 ataatgacgt tttggtgggc aatttcagaa gagtaaatcg cgaacttttt ccgatagaga
     6361 gtttgaatat tcccgagccg gttttgaaaa ggcttaattt gcggcatgag aaatccggtt
     6421 taattttaat aactggcata acgggttcag ggaaaagcac tactctcgat tccatcataa
     6481 atttgaacaa tgaagaaaat gaaggccata tagtaattat tagcgatccc cttgagttta
     6541 tccataaatc caaaaaatgt atcatccgcc accgggaggt aggcggtgat gttctaagct
     6601 ttagggaagg cacgatccag gctctacgcc aagaccccga tataattatc gtgggcgaga
     6661 tgcgtgatgc gcctacgatt gctacggttc tggaagcaac tgattccggt cataaagtat
     6721 ttaccacttt gcacaccagt tcagccgttg aaagtattca tcgaattata gccgaatttt
     6781 tacccgagga acaagagcgc atcagactca ggttggctga tacccttagc gtagtaattt
     6841 cgcagaaatt agttcctgat agagataaca aattaaccct agtgaaagaa attctctctg
     6901 tcactgattc agtaaaagcc gcaattagga acaaaaatat tggcgaaatc tatcagatga
     6961 ttaccgaagg taaaaagtac ggaatggtta cgatggaaca agaccttcat aatctatata
     7021 ctaaaaaaat tataagtaga gaaactgctc tcgacttctc caataataga aaaagaatag
     7081 agcagttgct tgcctatcgg gaaatttgtg tataacctta ttgagcttca taaatgagta
     7141 aaaagtgaaa aattactatg gtattttcca ggatggatta gatatacaaa tagctcatct
     7201 cgtgagggat gaagatttaa ttaaaatcca aaatttaggc gaaacgactt tatctgaacc
     7261 tctctataat aaaaaagagg taaataagat ccccacgatt gaattaagcg ataccaacct
     7321 ggaaatacaa aataaccctg atctgaaaga ggaaggattt gatttaccgg aaatctcgga
     7381 attcgatgaa tttgaaagca ataaactcac ggaagagata accgaagtta acgaagaagg
     7441 ggggacttct acttcggaac tgcagcgatt tttaagcaat ttttcattag aaactggaaa
     7501 gatagcacta aatgcgaatg aggaacaaat atcttaccat atcttcaatt cgtcgttaac
     7561 cttctcggca attaagaaga aaataaagac cgaaatttta agccaggaag aattgaaatc
     7621 cagaaaatat gccattggat gcataccgaa ttatgacggt atgactctgg catttttaca
     7681 tcgaggtgaa ttcgatttac ttaatgcaat tctccaggta agaccgctat tatccccaaa
     7741 gagatacatt tttagttata ttgataccaa tgaaattgcc ttgatgaatt tgattagagc
     7801 taactataat ttccccgaga acgactacgt cttgatcttg tatgtgggtt ccgagtataa
     7861 agcgggcata gtgatgcgaa ataataccca cgttaaaacc tttaagatta tcggcgatac
     7921 cagttctagg tcggaaaaca tccgacaggt gatttattcc aagattatcc ttgagcagga
     7981 cgtttccgat attccaccgg ttcagcattt gatcttgact ggtaatatgg tccgagatga
     8041 agatatacgg tttttcagta gtaaattcgg cgaaacgacc gaggttagcc ggttcgaatt
     8101 taaaaatatc aatatcgcta ctataaaccg aaaatattct ccggaaaaca tcgcaaaata
     8161 tgccatacca atctcattgg cattaaaagc cgccgatcct aaaaataaag ctttctataa
     8221 tactaatctt ctaccgccca aaattatcga gagtcaaaaa cgatttaaaa tcggctggca
     8281 cggctttatc ctgtttattg caatatttta tttcacctat tccgctaccc taaaaaatct
     8341 ggaaaataac cgccagattg tagaacttca acgccagaat tataatattg agtccgagtt
     8401 aagacacaag aatcttatgt tcgcccgagc taatgaactc tctcagcaga ttaccggtaa
     8461 gcgaaaaagt atggataaga ttaagactat ttcaggcaat aagagtcagt ggcatcgtac
     8521 cataatagcg ttatcagatg ccttcgctaa aaactctatt agctggatta attatcttag
     8581 gagtgatact agcggtttta tggtccaggg cactaccaac cataaagaga atatcgccta
     8641 tattaccagg tattttccag gatgtatagt ttccgatgtc aaaacctcgg agatccaatc
     8701 tataccggtg atgcgatttg tgatgaattt ttcgtactct gatcaggagg aatgggtaat
     8761 gaaagtcgct aaaccagctc cagttactcc gcaatccgga aaaaccgccg ctccgccccc
     8821 cataactcca gcgccaaaat caattgagag tgaaagcgct cccaaattag ccgcacctgc
     8881 gcctgccgtt gttcaaagtc agtcaccacc agctcaatct accccaagga ttactcctta
     8941 tactgttttc cgcgaagcgg cggagaccta tctatcgggc aatgtacagg aaggttatca
     9001 caagctcacc cagtttgtag agaaatatcc gaatagtccc tttacttata gtgctaatta
     9061 tttaattggc gaatgtctgt tcctgatggg tgaagtagct caggcgaaag atatttttga
     9121 aaaaattgct aaccaggacg gtactaaaac tccagatgcc ctgttgatgt tggggagatg
     9181 ttacaacgca ctcggtgata aagccaaagc cattgactgt tggggaatgc taatcacaaa
     9241 gtatccagaa cataaattag caaaaatggc tgaggaaaaa ataaaatacg taaccgaaca
     9301 ttaatatgcc ctataaaata agaaatacaa tagccttggc agtgatctta ctgatagtac
     9361 taataggaag tgtcatttca aattccagct cagagagaaa aattgccgat ttaagtaaga
     9421 aaaatcagga actttcaaaa cgcctgaata tgctcaatag ccaactggaa aatattggtg
     9481 aagaagagga actagagcaa gaaatcaggg aattagaaat cgaaatatct aaatttaaaa
     9541 aagccattgt gaaagaggat aatccgacga ttacttacga ttatcttacg cagatatgcc
     9601 agaaattttg tccggaaatg gtttttgatt tcaaaccggc cgaaagtggc gagattcaag
     9661 gaacaaagta taatgattat ttaattgctg ggattgttcc ctttaattct ttatatactt
     9721 ttatctatca aatagagaat caacccaaac tttatcttgt ggagttatta gaaataagag
     9781 aacagccagc tagtgaggat ataattccct tcgcattcaa attacgtgca ttttatgatg
     9841 agaacgggat tgagcctgaa agtatcccga taagagagat taaaacggtc aacattaggt
     9901 ttaatccttt ttatcccggg atccatgacc cagtgcaaat aaaggaagaa gaacagtatt
     9961 taaatccaga agaagcaatc ctagtaggat taactccaac tattgccttt tttaaggata
    10021 agaacggccg caccataagc ctgacaactg gcgaaaaagt agcttatggc tatttatcaa
    10081 aaatcgattg ggagaaccaa gcggttatct ttaaaataaa tagaattggt gtttatgtag
    10141 aaaaaaccat ctacctgcag ggaaaaggat aaaatatgaa gaaaaaagta ttatttattt
    10201 gtctattgtt gtttttcagt tcaactaact ctcaggtaaa tctcattaaa agtccttcta
    10261 gtgatgaact gattactatt tcacccgaag tactggtagc cgatgctctt aaaatgatcg
    10321 aagcgctatc acagaaatac gaagggcgta agatcgtgaa tatgagcacg tataccggac
    10381 cgattagcat tcctattact cagatttact ggaaaaacgc cttaaattta attgccaatt
    10441 ataacnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncggaa gtgcttagtg
    10561 atataatttc gggaacggtg tccactacct tagaatcggg agaagctatt attgaattaa
    10621 atgcactctt caagatgata gaacactata ataaaggtac cattatcgcc cgacctagtg
    10681 tcacggtaat tagtgggaag caaggatatt tacagattgg tcaagatttt tccgtgcgaa
    10741 ccaccgattt ttctggcaac ctagttgatc ggttttattc cgccgggctt atactggagg
    10801 taactccgac gattattcaa gatagcagta aagaagcaat acatttggtt gccagagttg
    10861 aaaaaagtag tgttgtacct tcggatgtgc aagtaatcgt taataaaaat caagccacta
    10921 ccgaagctat tttatatgat ggagaagaga ccgtagttgc cgggctttat gacacggaag
    10981 atattgttgt tcgcaaagga gttccaatat taaaagacct gccctggtgg gttttgggat
    11041 tgcggtatat atttggttac aataatcacg agaaaaatga aagagaaatt ataattatcc
    11101 taaaagcaga gctcattgaa cctatcgaag taagaatggc gaagaaagaa tcagctagag
    11161 aattaatcga gaaatactat aaaccaaaag aacatattga taccctgctg ttcaaaaaag
    11221 tgcaagaatg aataattacc attggcaata atttaattag ggagtgtcaa ttatctgaga
    11281 ttggggagat tggatgaaga ataaattaaa aattacagga ctatttcttt ttttagcgat
    11341 ttcattagtg gtcattttcg actgtggcaa aattacttca cccggtggga tcgaaattcc
    11401 cgaaaatccc agaatcaaga ccatcgacga taataatatc gaagtcagct gggattatga
    11461 ccaatctgac caggacgttt tttttaccgt tgccagaaaa gtaggagatg ctaactggaa
    11521 tctcgaatat gctcagactt atgataattt tttgatagat gaaatcccaa ctaatgatag
    11581 cctaatctat gcctataaga tcaagaccga aagtgataca tcggaattgg catcggaatt
    11641 ttccgacgta gtagcctatt tttcaccaaa aacgattcct acggaaatag ctatttcggt
    11701 cctcgaatct gataaggttg aggtgacttg gcgggataac tgtattggcg aagatggtta
    11761 taaaattgag aagaaagtca gcggcgggaa ttggattaaa gattataaaa ccttcggcag
    11821 aaatgtgact tcctttaccg ataaaacttc cttcggcgat tcggtttttt atcgagtcta
    11881 tgccttctgc ggcatatcgg tatcgggcag tgcggaaggt tttggcattt ctacgtttcc
    11941 gcctccctat aatcttgaat atgaacgaat tgctatagat aaaatcaaac tgacctggca
    12001 aagtaaaaca tccaatgtcg caggctttaa aatagataaa aaggtcggta gtgcagattg
    12061 gcaggttgag atagcaacgc tcgatccaaa tcagacctgg tggattgatg agaatgccga
    12121 aatcaatgag atactccagt accgggtata tggattttca gaagagtata tttccagcta
    12181 tgttactacc gatgaaatag acaatacctt tccggcgcct tctaatttaa ccttaacgaa
    12241 aattgatgag gatattgtga aaattagctg gcaggataat agtcaaggtg aatccggttt
    12301 ttatatcgat cgaaaagttg gaatgtccga ttgggtaaat ggatatgctt cggtagatag
    12361 caaccaaaca acattattgg ataatatctc agaaccatgc gggaattttt attatcgggt
    12421 aagagcattt aaaaatcaat atttttcaga acctactgat gagggaaata ttaacatacg
    12481 gttaaactta gtcagttccc taaccactga aggtactgcc actgaagtat ttgtctcgga
    12541 ttggaaagcc tatgttgcta atcattacaa tggtttaata gttatagatt tgataaatcc
    12601 atcttcaccc tctaccgata ataatttggt tttaccttta cccgatcgca ccttgtcaat
    12661 ttatatctat aaaaatttag cctatgctac taatcacact ggtggcttga atattgttga
    12721 tgttactacg ctggaattaa tcggtaattg ccagacaagt ggagtccctg ttgatgtttt
    12781 tgtaacgggt gaaccacctt atgcttatct ttgcgaagaa gagaaaggtg tgtccatcgc
    12841 ttcaattctg agtagtacac cacatataat ttcaaatatt acacctccca atacctctgc
    12901 tgatgaaaaa gccaaaaaat tgtttgtagc taatgattat gcttacgtgg cttacggttc
    12961 gcgaggcttg acaatatttg atgtttccga tgttaagaat ccgatatatg ttgcggattt
    13021 tcccagctcg agtattactc aagatgtgtt tattaaagat aattatgcgt ttcttgctga
    13081 cggtgaaaat ggattagtga tagttgatgt atcgagtgta gaagcaccgt ccttggtgtc
    13141 aaactgtccc acccacggtt ttgcttatag tatctacgtg cgtggcaatt atgcttattt
    13201 agccgataag gaaaaaggct taatcgtggt taatatttcg gatttggcta atccttttat
    13261 cttaggtagt tttgaaatgt caaccactcc caattcggtt tttgtagatg gcagttacgc
    13321 cttcgttacc gatagtgaag gattgaaaat cattcaaatt gccccttaaa agataaaatg
    13381 atagcagata tatttttgcc gacagtattt aggactaatc tcaaccccga agtattgaga
    13441 ttagataaca ggagttcctt gtattaacgg ttccatgctg aatcttataa aaaccttcaa
    13501 ttggggcgta aatattttgt tagaaaaagc tattt
//
LOCUS       DAOOMU010000062        13158 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_5211_length_13158_cov_23.005648, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000062 DAOOMU010000000
VERSION     DAOOMU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5211_length_13158_cov_23.005648"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1502)
                     /locus_tag="PLI55_06900"
     CDS             complement(<1..>1502)
                     /locus_tag="PLI55_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPB00600.1"
                     /translation="VYGIQAAAQRFFDKNSEQLTPDECALLAGIIKNSVRFSPIRNPI
                     NAYQRRNLVLHRMHKYGYLSDEEYALYKNTPLQVFKPELPPERAPYFVEYIRQWLQRE
                     DEKLGIDIYRDGLSIYTTIDSKIQAAADTALTRHLQYQQNLLNSRLLSNPSELRDFIK
                     DTTISLAQVRDMIRGNIPMSPSMQASLVVQGALVAIDPATGHILAMIGGRDFRESQFN
                     RATQAKRQPGSAFKPIVFAAAIDNGFPVTTQLLNQPVVLNMPDGSRWAPKNYDLSTGG
                     PTTLREGLAKSLNLISVRVIQELISPTSVVELAKRMHLTTNILPVDALALGACEVIPI
                     ELTSAFGIFENHGVWVEPIAITRIDDRYGNTIAQYKPRQELVLSEETAYLVTSLLESA
                     INVGTGAGARSVYGFRLPAAGKTGTTNNFNDAWFIGYTPYMVAGVWVGVDNPAISLGN
                     RQSGAAAALPIWANFMRESHNTKGWKAKNFERPDGIVEVQICSETKLLPST"
     assembly_gap    1503..1602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1734..2552)
                     /locus_tag="PLI55_06905"
     CDS             complement(1734..2552)
                     /locus_tag="PLI55_06905"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPB00601.1"
                     /translation="MKLKAEPEKISEAVEFLQKRLKNQSAKIAIVLGSGLGYFAEQLE
                     IVDRIDQQEIPHYPTSTVVGHAGKFIICNISGIPLIAIQGRTHLYEGRTLAEVTFYVE
                     ILARLGIRYLILTNAAGGIDPKLKPGSIVLLTDYISFTQIPLTPDGQSQTASPFCAEM
                     IAMVREIALQNKIELHEGIYCWTLGPSYETPAEIQAARKLGATVVGMSTIPEAVTAQR
                     LGLKVLGISLVTNLAAGISESPITHEEVQVTANRSKEVYSILMEKIIIELAKND"
     gene            2959..3192
                     /locus_tag="PLI55_06910"
     CDS             2959..3192
                     /locus_tag="PLI55_06910"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HPB00602.1"
                     /translation="MDPFSWSYTFSMYLRAIGWAFVAALGFSLGIGLAIKVFDLLSKD
                     INEWEEIKKGNIGVALIIVSLILMVGILVYKVI"
     gene            3541..4002
                     /locus_tag="PLI55_06915"
     CDS             3541..4002
                     /locus_tag="PLI55_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPB00603.1"
                     /translation="MELDKILTPRLIVYPLSAETKEEVISILVERLHDEGLISNPEAA
                     YQAVIEREKLMTTGIGKGVALPHGKYRETMEVLVAAGVSPEGIDFDSIDAQPVHIFIL
                     LLTPERFPSKHLKLLSKLSRMLNNANCRAEILAAQSANEIADIFYKYDSQV"
     gene            4012..4347
                     /locus_tag="PLI55_06920"
     CDS             4012..4347
                     /locus_tag="PLI55_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00604.1"
                     /translation="MPFCPRCLYEYVEGTSKCIDCNAELVARLPEEKMTDVKWFCLGV
                     LSDIVMAEMVKESLENNGIETILKIDPLHTTFSLQSTGATGSYAKLFVPCGKKKTALD
                     ILKSMSTTG"
     gene            4382..>4690
                     /locus_tag="PLI55_06925"
     CDS             4382..>4690
                     /locus_tag="PLI55_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPB00605.1"
                     /translation="MLYQRVKGTRDILPSEVHQWQFLENFLREYFKRNNYKEIRTPAF
                     EVTELFTRSIGGDTDVVSKEMYTFLDKGGTSITLKPELTAPVMRAYIQNNLEQISPLT
                     K"
     assembly_gap    4691..4790
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4791..5519
                     /locus_tag="PLI55_06930"
     CDS             <4791..5519
                     /locus_tag="PLI55_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HPB00606.1"
                     /translation="AIEPYHAELCGACQERLQKNVLRLFDCKVEICRQIMAEHAPSIL
                     DYLSNDDRLHFKKVCHLLDVSNVPYTIDQALVRGLDYYTRTTFEITSPLLGAQDALCG
                     GGRYDHLIADLGGKPTPGVGVASGIERLLLVLEQVGSFPEPTDNLVYVITMSENSRTA
                     GFRLVTELRRKKVNAQMDFLRRSIKAQLRDADREQANWVVIIGEDEISRNIVIFKDMK
                     NGDQRELPLNTAADQIVALLQDKT"
     gene            5565..>6697
                     /locus_tag="PLI55_06935"
     CDS             5565..>6697
                     /locus_tag="PLI55_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HPB00607.1"
                     /translation="MSFLNPGFLWLLPLAGIPLLIHLLGRQRFQVVEFSSLRFLKSLQ
                     ADVLRRLKIRQIILLIIRTLLILCLILIFARPYRSSQTPGIYMAKGANLYLIVDNSAS
                     MSLKCDGRTQLSRGIEAILSSAKNIDFPVNLNLILATETAGISNRTTVYSLSDLEREL
                     VHTPETNFAAQLLPAIALAVEEIIKNYDLNGVIWLVSDFQASNWNRKSPMEQKLLEEI
                     IANKIRLVLFPVTGEADNSALGAIHFNEQIYAKDKPVTLQSTTINWRRQPQENLVSLF
                     IEEERVAQALINLPPAKSEQATFEFIPLNSGILSGYLQIADDDLAVDNRRFFVLNIPA
                     TLRILVVGSQATDGDYIMKALQANPDQMIEARFTTSEYLGNEIL"
     assembly_gap    6698..6797
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6798..7487
                     /locus_tag="PLI55_06940"
     CDS             <6798..7487
                     /locus_tag="PLI55_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00608.1"
                     /translation="ENVSPTATPRFFKAPGLICGSKHKILMSFDDNTPLMVETHYEAG
                     RAVMMATVPKADWSDLPFSGFFPVILQRMTQYIAGGATQPLNYTNGDTVKIARIPGEN
                     SIPQVQTPSGRRFNPSVVSNYFSFDNTAEIGIYSVIREGGVIEKFAVNLPLSETAAEF
                     FSVADYKNLIKNAPANITVVSETSTDEISQLTLTREFSTLLLFCALFLAIAETFIGRL
                     NRIEMVNPPAE"
     gene            7484..8797
                     /gene="hflX"
                     /locus_tag="PLI55_06945"
     CDS             7484..8797
                     /gene="hflX"
                     /locus_tag="PLI55_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPB00609.1"
                     /translation="MSKLPDQSKIFPTGAVLPSEKVLLVGIINSNQPRHITEEHLTEL
                     ELLMNTLGYEMADKQFVNQRAVDPAYFIGRGKANEIRDFLKSQNISEIVFDDDLSPTQ
                     MRNLQEFLDVEVRDRTGVILEIFASHAHTNEARTQIELATLEYLLPRLTRRWTHLERQ
                     IGGIGVRAGSGETQIETDRRLIKTRIAKLKSELVAIDRQRTVQSQRRANCFRIALAGY
                     TNAGKSTILNLFTGADVLVEDKLFATLDTTVRQLNLDGYPNVLLSDTIGFIRKLPHNL
                     IASFRSTLREIIDADLIIKVADISSPHCLQQLESVEEVLNELGAGNRPTLIVFNKIDR
                     LDENVFKIAQKTYPEAVFISALNQLRIDDLIQALERAIAREGRHLRIQTPFEEVSDCA
                     LIRNHTTVQQEIYEDDVVIFEVFCYKNVWNWLARQLSSATTVLEM"
     gene            8979..9812
                     /locus_tag="PLI55_06950"
     CDS             8979..9812
                     /locus_tag="PLI55_06950"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPB00610.1"
                     /translation="MNDFAGKRFNLFGASRVGVSLAYHLNRLGFKPEFVWNRSPERLA
                     QAKQAVPFQNFSTDLTAACRDKPDWIIIAVTDDAIEEIATQISQCLPDFKAVKVFHTS
                     GFLSSEALSSLKRKGAATGSLHPVVSVPDITTGIRLLGQCVYTCEGVIKKSLAEIVRS
                     IGGQPYLLNRKQKETIHLSAVFLNNYVVALINAIKILNSESNLEPSKAQAILRPITEQ
                     AVLNGWEKNVADALTGPLMRGDYQTIEKHLALLNNYPELKKLYQDFIDLALQVVAHSK
                     R"
     gene            10043..11404
                     /locus_tag="PLI55_06955"
     CDS             10043..11404
                     /locus_tag="PLI55_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB00611.1"
                     /translation="MDKKENLPIVIYGGPLFDGINTVYEDGAVYISNGIVEKVGNESE
                     VFPQVTNTEQVEIYDTQGKVIFPGLINLHHHFYAALAKGLSPVGPVTNFQEHLENLWW
                     RFDRALDPEAVQLAALLSLLDCIKSGVTTIFDHHSSPSHVKGSLDNIAAVVERSGLRA
                     CLCYEISDRNGRKTFQDSLQENLAFIESHRDQRRLRGILGLHANFTLSEKSMVEIAQY
                     YDPEIGIHIHCAEDKIDREYCRELGYDGPVSRLKHFGLLGKKSILAHGVHLDQAELTE
                     VARSGSIIVHNPESNADNSVGRLNLPPADDVSIGLGTDGMTSAMLATCRAGYLIQRQA
                     GVADTQILNYLLQCLFKTNSTFSSQFIGFQLGQIVAGAAADITVFDYIPVTPFNSDNI
                     FRHIIYGMPSARADMVMVDGQQIYTDGVFLTLDEELIREAAQKASQRVWKQFAETNVL
                     NFK"
     gene            11447..11707
                     /locus_tag="PLI55_06960"
     CDS             11447..11707
                     /locus_tag="PLI55_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00612.1"
                     /translation="MTKSFMIIVVNLRKKEAVNVQKILTEWGCLIRTRLGLHEGVLDQ
                     CSDVGSIILELVGERDKHIELARKLNLLSGVKAQYIEIPVEM"
     gene            11733..12710
                     /gene="pfkA"
                     /locus_tag="PLI55_06965"
     CDS             11733..12710
                     /gene="pfkA"
                     /locus_tag="PLI55_06965"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013925878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPB00613.1"
                     /translation="MGVLKRIAILTSGGDAPGMNACVRAVVRTAIYDGIDVFGVYGGF
                     EGLINGDVIPLNRRSVSNMIQKGGTFLKTSRSEAFRHKSGRKRAAEHLAEWKIDGLIV
                     VGGDGSFRGTHDLYEESGIQVVGIPATIDNDIYGTDITIGYDTAVNTALDSIDKIRDT
                     AASHDRLFIVEVMGRSAGFIALEVGICGGAEEILVPETKVTLENLGNLIKTWAIAGKT
                     SSLIVVAEGDELGNAHEIAAKLYQQYHTICHVCVLGHIQRGGSPTATDRLLASRLGYH
                     AVKALEDGKSDVMVGWINNSVTFIPLPESWEKKKPLDEELIKIYRILST"
BASE COUNT         3591 a         2678 c         2933 g         3656 t
ORIGIN      
        1 gttgagggca ggagtttagt ttcggaacag atttggactt cgacgattcc gtccggacgt
       61 tcaaaattct tagctttcca gccttttgta ttatgagatt cccgcataaa attagcccag
      121 atcggtaaag cggcggccgc gccggattgg cgattgccga gagaaatagc cggattgtcc
      181 actcctaccc agactccggc taccatatag ggagtataac cgatgaacca ggcgtcattg
      241 aaattgttgg ttgtaccggt tttaccggcg gctggcaatc ggaatccata aacactgcgt
      301 gcccctgcgc cagtacctac atttatagcg gactccagta aactggttac caaataagcc
      361 gtctcttcgc tcagaactaa ttcctgacgc ggtttatact gagcaatcgt attgccataa
      421 cgatcgtcaa tgcgggtaat agcgattggt tcaacccaaa ctccgtggtt ttcgaagatt
      481 ccaaaagccg aggttaattc aatcgggatg acttcacaag cgcccagtgc tagagcgtca
      541 accggtaaaa tattagtagt caagtgcata cgtttcgcca gttcgacgac tgaagttggg
      601 gaaattagtt cttgaatgac gcgtacggaa attaaattga gtgacttagc caatccttcg
      661 cgcaaagtgg taggaccgcc ggttgacaaa tcataatttt tcggtgccca gcggctaccg
      721 tccggcatat tcagcaccac cggctgattg agtaattgag tggtcacagg gaaaccatta
      781 tctatggcgg cggcaaagac aatcggtttg aaagccgagc ccggttggcg tttagcctga
      841 gtagcgcgat tgaattggga ttcacgaaaa tctcgaccac cgatcatagc taaaatatgt
      901 ccggtagcag gatcaattgc caccagtgct ccctgaacta ccagactggc ttgcatactg
      961 ggactcatcg gaatattacc ccggatcata tcgcgcactt gcgcaaggga tatggtagta
     1021 tcttttataa aatcacgtaa ttcagagggg ttgctgagta accttgaatt aagcaaattt
     1081 tgctgatatt gtagatgtct ggtcaaggcg gtatcagccg ccgcctgtat ttttgaatca
     1141 attgtggtat agatggacaa accgtcgcgg taaatatcaa tcccgagttt ctcatcctcg
     1201 cgctgaagcc attgccgaat gtattcaacg aaatacggag cacgttcagg cggcaattcc
     1261 ggtttgaaaa cctggagagg tgtatttttg taaagagcat attcttcatc ggaaaggtaa
     1321 ccgtatttgt gcatccgatg cagaacaagg ttacggcgct ggtaggcatt gattggattg
     1381 cggatcggcg agaaccggac ggaatttttt attattccgg ctaataaagc acattcgtcc
     1441 ggggtaagtt gctcggaatt tttatcgaag aagcgctgag ccgccgcctg aataccgtaa
     1501 acnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncgagtaaa gtaatgcttt
     1621 cggccagcta agcagttcag tttctgtttg tttagtgatg cgtcccatca gttttatggt
     1681 attttaaatg aacaaattca ggaaaacaaa aatcgagcta aatatagaaa aattcagtca
     1741 ttttttgcta actcaattat tattttttcc attaaaatgg aataaacttc cttactccta
     1801 ttagctgtaa cctggacttc ttcgtgagta atcggtgatt cactaatgcc agccgccaga
     1861 ttagtgacta agctgatacc gagcactttc aagccaagcc tttgggctgt gacggcctcg
     1921 ggaatagtgg acataccgac gacggtagcg cccaatttgc gagcggcttg gatttccgct
     1981 ggggtttcat agctaggtcc gagagtccag cagtagatcc cctcgtgcaa ttcgatttta
     2041 ttttggagag cgatttctcg taccatagcg atcatttcag cgcaaaaggg cgaagctgtt
     2101 tggctttgac catccggcgt tagtggtatt tgggtaaaac taatataatc agtaagtaga
     2161 actatactac ccggttttag ttttggatct attccaccag ccgcattggt cagaatcaag
     2221 tagcgaatgc cgaggcgggc taaaatttcg acataaaagg ttacctcagc cagagtgcgt
     2281 ccttcataga gatgagtccg tccttggatg gcgatcaacg gtatgccgga gatgttacaa
     2341 atgatgaatt taccggcgtg accgacaacc gtagaagtcg gataatgtgg aatttcttgt
     2401 tgatcaattc ggtcaacgat ttcgagttgc tcagcaaagt aacccaaccc cgatccgagc
     2461 acgattgcta ttttggcact ctgatttttt aaccgttttt ggagaaattc aactgcttca
     2521 gatattttct ccggttcagc ttttaatttc attttcaaaa tgctctatcg ttttattaaa
     2581 attgcgtcgc aaattagaca caataattca acttggcaat aaaaactagt ggtgaaaggc
     2641 cgaaataact aaaccaaatg ggaactagat aaattgagca gaagtttgaa attcgatcag
     2701 aagaatgatc cgattgataa tacattttct aatcaaaaaa gaaccctcct atatcttagg
     2761 ctttcttcga ttactggcga ctggaagaaa tctcccataa tcctgccctg ctttaatttg
     2821 gtctcattgg aaaaaagtag ttgataaaaa aaattgatta ttagtaactt aattacggat
     2881 cgaatcataa gattggcaat aaatatttga aaagaaaaaa ctcaattaga atcaataaaa
     2941 ctcaaaagga ggtatcaaat ggacccgttt tcctggtctt atactttctc aatgtatctt
     3001 agggctattg gctgggcatt cgtagccgcg ctcggtttct cgctcggcat cggtttggct
     3061 atcaaagtct ttgacctgct gtcaaaagat attaatgaat gggaagaaat taagaagggc
     3121 aacataggcg ttgcgctaat tatcgtgtcc ctgattttga tggtcggcat tctggtttac
     3181 aaagtcattt aagttaaatt ctttttaaaa atgtagtaaa aaagccaata gttcaggctg
     3241 gtgtttttta aatgactgaa gttctcttct aattatattt tgagatattc tgccgcattt
     3301 gactggtttg aactgtttta acatccctct tttgcatgtt agaaaaggtc gggttatcgg
     3361 tgccagtgat cggctatatt ttgcactcgg cggctacgat ctaaaaccga tgattttgtc
     3421 catttatttt gactgttttg aaaattggac tatccgttat atttttggaa tgattaaagt
     3481 tgtgtaaaaa tgctttatac tataaatttg ttaagatttt tgaatgagaa gtaaagtcta
     3541 atggaacttg ataaaatttt gaccccgcgc ttgatcgtct atccactttc ggcggaaacg
     3601 aaggaggaag taattagcat attggtggaa cggttacacg atgaaggctt aatttccaat
     3661 ccagaagccg cctatcaggc cgtcattgag cgcgaaaaat tgatgacaac cgggatcggc
     3721 aagggagtcg ctttgccgca tggcaaatac cgcgaaacaa tggaagtgct ggttgcggct
     3781 ggtgtttcgc cagaaggcat tgattttgat tcgatcgatg cccaacccgt gcatattttt
     3841 attcttctct tgactcccga aagatttccc agcaaacacc ttaaattgct cagcaaatta
     3901 tcgcgtatgc tgaataacgc caattgtcgg gctgaaatct tagccgccca atcggcgaat
     3961 gagattgccg acatttttta taaatatgat tctcaagttt agaggttagt aatgccgttt
     4021 tgtccacgtt gtttatatga atacgtcgag ggcacctcaa aatgcattga ttgtaatgct
     4081 gaattggtcg cccgccttcc agaagagaaa atgactgatg tcaagtggtt ttgtctcggt
     4141 gtcttatcgg acatcgtaat ggcggagatg gtcaaagaat ctctcgaaaa taatggtatt
     4201 gaaactatcc tgaaaataga cccgctccat actacttttt ctctgcaaag caccggcgcg
     4261 accggctcct acgccaaatt gtttgtgccc tgtggtaaga agaaaactgc tctggatatt
     4321 cttaaatcta tgagtaccac aggttgacca gcttaaagca aaatatgagt tgataatcaa
     4381 tatgttgtat caacgtgtca aaggcacccg cgatattctt ccttctgaag ttcatcaatg
     4441 gcaatttctg gaaaatttcc tacgtgaata ttttaagcgc aacaattaca aggagattcg
     4501 taccccagct ttcgaggtca cagaattatt cacacgcagt attggcggcg atacagatgt
     4561 agtttccaaa gaaatgtaca cttttctcga taaaggcggc accagtatta ccctgaaacc
     4621 tgaactcact gcgccggtta tgcgcgccta tatccagaat aatttagagc agattagccc
     4681 gctgactaaa nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gctatcgaac
     4801 cttatcatgc tgaactttgt ggcgcttgcc aggaacgtct ccagaaaaac gtcctgcgtc
     4861 ttttcgattg taaagtcgaa atctgccggc aaattatggc tgaacatgct ccttcgatac
     4921 tcgattatct gagcaatgac gaccgactac attttaaaaa agtatgtcat ttactcgacg
     4981 ttagtaatgt tccctatacg attgatcaag cgctggtgcg tggtttggat tactacacgc
     5041 ggaccacttt cgaaattacc tcgcctctct taggtgctca agatgccctt tgtggcggtg
     5101 gtcgctatga ccacctgatc gccgatctcg gtggcaagcc aactccgggc gtcggagtgg
     5161 cgtccggtat agaacgtctt ttgcttgttc tggaacaggt agggagcttt cccgaaccga
     5221 cggacaactt ggtttacgtt attacaatga gtgaaaactc tcgaacggcc ggctttcgac
     5281 tggtgaccga actacgccgc aaaaaggtca atgctcaaat ggatttcctg cgtcgttcga
     5341 tcaaagctca gttgcgggat gccgatcgcg aacaagctaa ctgggtagta attatcggcg
     5401 aagatgagat ttcccgcaac atagtcattt tcaaagatat gaaaaatggc gatcagcgcg
     5461 aattgcctct caatactgcc gctgaccaaa tcgttgccct tttgcaagat aaaacctgag
     5521 ttgaatttac ttcacggaaa taaattccag ccgcaggaat cgaaatgtcc tttttaaatc
     5581 cgggatttct ctggctcttg ccgttggctg gcattccttt gctgattcat ctacttggtc
     5641 gtcaacgatt tcaggtggtg gaatttagca gtctgcgttt tttaaaaagt ctccaggctg
     5701 acgttctccg tcgtctgaaa attcgacaaa ttattctgct aattatccga actctgctga
     5761 ttttatgcct tattctgatt tttgccagac cgtatcgtag tagtcaaacc cctggaattt
     5821 atatggcgaa aggtgctaat ctatacctta tcgttgataa ttcggcttca atgagtttga
     5881 agtgtgacgg gcggacgcaa ttatccagag gcatagaggc aatcctatcg agcgctaaaa
     5941 atattgattt cccggttaat ttgaacttga tccttgccac ggaaacagct ggaatcagca
     6001 accgaactac ggtttacagc ctctctgatt tggagcgcga attggttcat accccggaga
     6061 ctaatttcgc cgcacagttg cttccagcaa tcgctttagc agtcgaggag ataattaaga
     6121 actatgacct gaacggtgtc atctggctcg taagtgactt ccaggctagc aactggaata
     6181 gaaaatcacc aatggagcaa aaacttctgg aagaaataat tgccaataaa atccggttag
     6241 tgcttttccc ggttactggt gaagcggaca actcggcgct gggcgcgatt cattttaacg
     6301 aacaaattta tgccaaggac aaaccggtta ctttgcagag tacgacaatt aattggcgac
     6361 gacaacctca agaaaacttg gtttcactat ttattgaaga ggaacgagtt gctcaggcgc
     6421 tgattaattt gcctcccgcg aaaagcgagc aagcgacttt tgagttcatt ccgctgaatt
     6481 ccgggatttt gagtggctat ttacagattg cggatgatga tctggcggtt gacaatcggc
     6541 gtttcttcgt cttgaatatt cctgcgactt tacgcatttt ggtcgtgggt agtcaagcaa
     6601 ccgacggcga ttatattatg aaagcattgc aagccaatcc cgatcagatg attgaagccc
     6661 gctttactac cagcgaatat ctcggtaatg aaattctnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnngaa aatgttagcc cgaccgccac tccgcgtttt tttaaagcac
     6841 ccggtttaat ctgcggatca aagcataaaa tattgatgtc cttcgatgat aatacaccgc
     6901 taatggttga aacgcattac gaagccgggc gagcggtaat gatggctacc gttcccaagg
     6961 cggactggag cgacctgcca ttttccggat ttttcccggt tattctgcaa cgtatgactc
     7021 aatatattgc tggaggtgct actcagccct tgaattacac aaacggagac acggtcaaaa
     7081 tcgccagaat tcccggtgaa aattccatcc ctcaggtgca aactccttcc ggacgtcggt
     7141 tcaacccgtc ggttgtgtct aattattttt catttgataa tacagccgaa attggaattt
     7201 attccgtgat tcgggaaggt ggtgtgattg aaaaatttgc cgtcaatttg ccgctcagtg
     7261 aaacagcggc tgaatttttt agcgtagccg attataaaaa tttgattaaa aatgctccgg
     7321 cgaatattac cgtggttagt gaaaccagta ctgatgaaat cagccaattg acattgaccc
     7381 gcgaatttag cacgttgcta ctgttttgcg cattgttttt ggccattgcg gaaacgttta
     7441 tcggtcgttt aaatcgaatc gaaatggtaa atccacctgc cgaatgagca agttgcccga
     7501 tcaatctaaa atttttccaa ccggcgcggt tctaccatct gaaaaagttc tcctggtggg
     7561 cataattaat tccaatcagc cacgccatat aaccgaagaa catttaactg aattagaatt
     7621 actaatgaat actctcggtt atgaaatggc tgataagcaa tttgtgaatc aaagagccgt
     7681 tgatccagcc tatttcatcg gccggggcaa agctaacgag attcgtgact ttttaaaatc
     7741 ccaaaatatc agcgaaatcg tatttgatga tgatctttcg ccaactcaaa tgcgtaattt
     7801 gcaggaattt ctggatgtcg aagtgcgcga tcgcaccggt gttattctcg aaatttttgc
     7861 cagtcatgcc catactaatg aagctcgcac tcagattgaa ttagccacac ttgaatatct
     7921 tctgccacgc ttgacccggc gctggacgca cctggaacgc caaatcgggg gcattggcgt
     7981 ccgagccgga tccggcgaaa ctcaaattga aaccgaccgc cgtttaatca agacccgaat
     8041 cgcaaaactc aaaagcgaac tggttgctat tgatcgccag cgcaccgttc agagccagcg
     8101 ccgcgcgaac tgttttagga ttgccttagc cggttacacc aatgctggca aatcaacgat
     8161 tcttaatctt ttcaccggtg ctgatgtttt agtcgaagat aaactttttg ccactctcga
     8221 tacaacggtt cgccagctga atctcgacgg ctatccaaat gttttattaa gcgatacaat
     8281 cggattcatt cgcaaattac ctcacaacct gattgcttcc tttcgcagta ctttgcggga
     8341 aattattgat gctgacctga ttatcaaagt cgctgatata agttcaccgc attgtcttca
     8401 gcaattggag tcagtcgaag aagttttgaa cgaactcggc gctggtaacc gcccaacgtt
     8461 aattgttttc aataaaattg accgattaga tgaaaatgtc tttaagattg cccaaaaaac
     8521 ttatcctgaa gcggtattca tctcggcgtt gaaccaacta cgaattgacg atttgatcca
     8581 ggctttggag cgagctattg cgcgggaagg tcgccacctg cgaattcaga ctccgtttga
     8641 ggaagtgtcg gactgcgctc taattcgcaa tcacaccact gttcagcagg aaatctatga
     8701 ggatgatgtg gtaatattcg aagttttttg ttataaaaat gtctggaact ggcttgccag
     8761 gcaattgagt tccgcgacaa ccgttttaga aatgtgacga ttttaaccgg ttaacggact
     8821 tgttatttag ctagcgactg tttactttaa accaaatgaa gcggtggaat agatgaaggg
     8881 aaatacagtc caaattcgca agataatggg agcgttttat tccaaaatgc cgatgattcc
     8941 aattcgaaaa gtcggcggat atttcttggc gatccaatat gaatgatttt gcggggaaaa
     9001 ggtttaatct tttcggagcc agtcgagttg gcgtttcgct ggcttatcat ctgaatcggc
     9061 ttgggttcaa accagaattt gtctggaatc gtagtccgga acgtttagct caggctaagc
     9121 aggccgtacc ttttcagaat ttttcaaccg atttgacagc cgcttgccgt gataagccgg
     9181 actggattat catcgctgtt accgacgacg ctatcgagga gattgcgact caaataagtc
     9241 aatgccttcc ggattttaag gcggtcaagg tctttcacac ttccggtttt ttaagttcgg
     9301 aggcgttaag cagtctgaaa cgcaagggcg ccgcaactgg ttctttgcac ccggttgtca
     9361 gtgtgccgga cataacaacg ggcattcgcc tgcttggtca gtgcgtttat acttgcgaag
     9421 gcgtaattaa gaaatcctta gcagaaatcg ttaggtcaat cggcggacag ccttacctgc
     9481 ttaatcgtaa gcaaaaagaa accatccacc tatcagccgt tttcttgaat aattatgtag
     9541 tcgctttaat taatgccatt aaaatactga acagtgaaag taatcttgag ccgtcaaaag
     9601 ctcaggcaat tctacgacca attaccgagc aagctgtcct caatggctgg gagaaaaacg
     9661 tcgcggacgc cttaaccggg cctcttatgc gcggcgacta tcaaacgatt gaaaaacacc
     9721 tcgctttact gaacaattac ccggaactaa agaaattata ccaggatttc attgatctgg
     9781 cgctccaggt agttgctcac tcgaaacggt aaatattttg tttaaattaa ttttaatggg
     9841 atttcgaata atcccgattc cggctcttga agagaattaa ttttttgaat gttcgataga
     9901 ttctactatt ttatatcgtg attatattag aaagtttaaa gtacgaattg aatttccagc
     9961 cagtcttaaa cggtaagtta gctttaaata ctaatggtgg cacattattc taatttattg
    10021 aacacagtta gcagaggcgt ttatggataa aaaagaaaat cttccaattg ttatttatgg
    10081 tggaccgcta tttgatggta tcaacacggt ttatgaagac ggagctgtgt atatttccaa
    10141 tgggatagtc gagaaagttg gcaacgaatc tgaggtcttt ccccaagtta ctaatacgga
    10201 acaggtcgaa atttacgata ctcagggcaa agtaattttt ccgggattaa ttaatctgca
    10261 tcatcatttt tatgcggcgc tggctaaagg gctttctcca gtagggccgg taacaaattt
    10321 tcaagaacat ttggagaact tgtggtggcg tttcgaccgg gcgctcgatc cggaagcagt
    10381 tcaattggcg gcgcttttgt ccctgttgga ctgcattaaa tccggggtaa ctacgatttt
    10441 tgatcaccac tcatcgccgt cgcacgtcaa aggtagcctc gataacatcg ccgcagtcgt
    10501 cgagcggagt ggcttgcggg cttgtctttg ctatgaaatt tctgaccgca acggccggaa
    10561 aactttccag gacagtttac aggaaaacct tgcctttatc gaatctcatc gcgatcagcg
    10621 acgattacga ggaattctcg gcttgcatgc caattttact ttatcagaaa aatcaatggt
    10681 tgaaattgct cagtactacg acccggaaat tggtattcat attcattgcg cagaagataa
    10741 aatagataga gaatattgtc gtgaattagg ttatgatgga ccggtcagtc ggctaaaaca
    10801 ttttggatta ctgggtaaaa aatccattct ggcgcacggc gttcatctcg atcaagcaga
    10861 actgactgaa gttgctcgga gtggtagtat tatcgtacat aatcccgaat cgaatgccga
    10921 taattccgtc gggcgattga atttaccccc ggcagatgac gtttcgattg gactgggtac
    10981 ggatgggatg acttccgcga tgctggcgac ctgtcgcgcc ggttatttaa tccaacgcca
    11041 agctggagtt gccgacactc aaatcctcaa ttacttactg caatgtttat tcaaaaccaa
    11101 ttccacgttt tccagtcaat ttatcggctt ccaattaggt caaattgtag caggtgcggc
    11161 ggcggatatt acggttttcg attatatacc ggttacgccg tttaactcgg acaatatttt
    11221 ccgtcatatt atttatggaa tgcccagcgc tcgcgcggat atggttatgg tggatggtca
    11281 acaaatctac accgacggag tatttttaac gctcgatgaa gaattaattc gtgaagccgc
    11341 ccagaaagcc agtcaaagag tttggaaaca attcgctgaa accaatgtgt taaattttaa
    11401 atgataatta gttcaacaat atattgctaa agtatcagga ggatctatga ctaaaagttt
    11461 tatgatcatc gtcgtaaatc tgcgtaaaaa agaagcggtc aatgtccaga aaattttgac
    11521 cgaatggggt tgcttgatcc ggacccgact cggtctgcac gaaggcgtgt tggatcaatg
    11581 ttcggatgtc ggttcgatta ttctggagct cgtcggcgaa agagataaac acatcgaatt
    11641 agcccgcaaa ctcaacctgc tatctggtgt caaagcccag tatatcgaga ttcccgttga
    11701 gatgtgagtt ttaaatttag aaaggtaaat atatgggcgt tttaaaaaga attgcgattc
    11761 tcaccagtgg aggtgatgcg ccggggatga atgcctgcgt aagagccgta gtacgaacag
    11821 ccatttatga tggtattgat gttttcggag tatatggtgg attcgaaggt ttgattaacg
    11881 gcgatgttat tccgttgaac cgacgttcgg taagcaatat gattcaaaaa ggtggcactt
    11941 tcctcaaaac ttctcgtagt gaggcttttc ggcataaatc gggtcgcaaa cgcgccgctg
    12001 aacacttggc tgaatggaaa attgacggtc tgatagtagt cggtggagat ggcagtttcc
    12061 gaggcaccca cgatctttat gaagaaagcg gaatccaggt tgtaggtata ccagctacaa
    12121 ttgacaatga tatttatggt actgatataa ctatcggcta tgacacggcc gtaaacacgg
    12181 ctctcgattc gattgataaa attcgggata cagccgcatc gcacgaccga ctcttcattg
    12241 tcgaagttat gggacggagc gccggattta ttgctttaga agtcgggatt tgtggtggag
    12301 cagaggagat tctagtgcca gaaaccaagg tgactctgga gaatcttggt aatttgataa
    12361 aaacctgggc gatcgccggg aaaaccagca gtctaattgt agtagcggaa ggtgatgaac
    12421 tcggtaatgc ccatgagatt gccgccaaat tataccagca gtatcacacg atttgtcatg
    12481 tttgtgtgct cggacatatt cagcgtggcg gtagccctac cgcaaccgac cgtttattag
    12541 ccagtcggtt aggctatcat gcagttaagg cacttgagga tggcaagtcc gatgttatgg
    12601 tcggctggat aaataactcg gtgacattta ttcctttacc ggaaagctgg gagaagaaaa
    12661 aaccactcga tgaagagcta ataaagattt atcgcatcct atcaacttga ttaggcaaat
    12721 tccaagcttt taaacggtat cctgcttaac gctagcgcta tttattggga atagacgggc
    12781 tagcgagttt ggttattacc tcaaaggtat tgttttaata ttacgaatag aaataatcta
    12841 tcaccatcgt cccagttgag ggatttcacc gtcaaaatga ctgaaaaatt gacctgaatg
    12901 tcacaagcgt gatagttcga actattcact ttaggatcgg aatgaattta aagcgttgta
    12961 aatatattgg aatacaatcg gcagacgttg ataaaaatat ggtaggaatt ggattcgagg
    13021 aaaagttaat tgcgcttaat ttgagaagta aatattgaat caactccgga atgaaacatc
    13081 aaaaatcgag aagaaacata gctaatctaa ccagtatcta cttattttcg ggctttggaa
    13141 aataatctcg tttttttt
//
LOCUS       DAOOMU010000063        13079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_5248_length_13079_cov_4.824401, whole genome shotgun sequence.
ACCESSION   DAOOMU010000063 DAOOMU010000000
VERSION     DAOOMU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13079
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5248_length_13079_cov_4.824401"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2160
                     /locus_tag="PLI55_06970"
     CDS             <1..2160
                     /locus_tag="PLI55_06970"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HPB00614.1"
                     /translation="IIFSCGQNQASEHPSLVISAEEGREIHATLGKYPILDKSYKKIV
                     DEVTVALAKPVDVPRPGEAGGYEHERHKQNYQEMKSAGILFTITGDEKYARFIKNILD
                     KYAELYPTLGPHPLSHDQKPGKLFHQMLNETVWLLNTAIAYDCIYNWLNNEDRQKYEE
                     NIFTPMVKWFTTEHPAEFNRIHNHGMWAAASVGMIGYVIKNQDYIDMALYGTNKDGTG
                     GFLKQIEMLYSPDGYYLEGPYYSRYAMEPLVYFSEAIERNQPKLKIFEYKDKIIKKAY
                     YAAVQSTFPNGVFFPINDASRTMNILAPGIIYGNSLVFFRYGTDINLLGIAKIQQEVP
                     LNALGLKLAQEYKSLKEVPDFSWKSCEFLDGFDGKQGGLGILRHGEGADQTVLIMKYG
                     VHGFGHGHFDMLHFMFYNQQREVIFDYGYARWINIEPKFGGRYLPENKSYAMQTIAHN
                     TVVVDETTQLKFNQKQADQMHGNRHFFDAENENIQVMSAKANDYYPGIKMQRTMFLIK
                     DVHLEYPVVVDIYRLSSSQTHQYDYPIHYHGQLINTNFKYTASTKVQKAMGDNYGYQH
                     IWSEAYAVVNQCPSITWLDGNRYYTQLTSYLPKTEVYFGRIGAEDPDFNLISEPMTII
                     RQSGKDHLFASIIEPHGYFDESREISYQARPIFKQIRVVGFNDKGSVIEVLGEGDIHW
                     RLMVNNLDASDKKEHILEFNGETYRWVGNYRVDLDVK"
     gene            2185..3456
                     /locus_tag="PLI55_06975"
     CDS             2185..3456
                     /locus_tag="PLI55_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB00615.1"
                     /translation="MKKVKGLRWWIIGMIALATVINYIDRGTLAIMWPSISKDLGMTK
                     QNYAFILNMFMIAYAIGQTVSGSLFDKVGTRIGYVITIAVWGVSSFLHSIARSIFSFS
                     FFRITLGLGEAGNWPGAVKSNAEWFPVKERAIAQGIFNSGASIGSVIAPPLIATLWAA
                     FGWKVTFMIVGCFGLIWIIPWLIINKSGPEKHPWITEAEREYILSGQPSVKNSRGEEK
                     GLKAKEILSHKEAWSVLVSRFFLEPVWWLFVGWMPIYLADKYGFNVQQIGYFAWVPYV
                     GAALGSIIGGGYAGRLMGRGKTVNSARKQAITIGGVIMFLGLLATIFFANTPLRFVII
                     VAFVLFGFQFTIGNVQTIPSDLFSGKSVGTLAGIGGSVVVFSVIIMNFLVPIISKISY
                     IPIFIMIAALVPLGVAAVYLLVKEIKPLNFK"
     gene            3476..3808
                     /locus_tag="PLI55_06980"
     CDS             3476..3808
                     /locus_tag="PLI55_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB00616.1"
                     /translation="MKTHIKNSDVPIEEVAWGMTRQVMGYDTNLMLVRVCFKKDAVGS
                     EHAHIHQQVTYIEKGIFEVNIGGKKEVLKAGDAYVIPSNVLHGAVCLEDGILIDTFSP
                     MREDFIKK"
     gene            3824..4471
                     /locus_tag="PLI55_06985"
     CDS             3824..4471
                     /locus_tag="PLI55_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HPB00617.1"
                     /translation="MNRQKILDRLKDSGIIAVIRLDGVENLDKIIDALAKGGVQALEV
                     TMTTPNAVAIIRDISFSLSEDFLIGAGTVLNTDTAKEVIDAGAQFVVSPVFKPSIIEI
                     VHQYDKVAISGAFSPTEILSAWETGADIVKIFPATVLGPVYFKDIHGPLPQVKLTPTG
                     GVNLDNAEEFIRCGAVCLGVGTALLDKKLIKNQDWDALEEKARQFKAAVERGRKG"
     gene            4475..5500
                     /locus_tag="PLI55_06990"
     CDS             4475..5500
                     /locus_tag="PLI55_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HPB00618.1"
                     /translation="MTAKIVTFGEIMLRLAPFNFERIVQANQFQVTYGGGEANVAVSL
                     AQLGDESYFVTRLPDNPLGEAAWKTLCGFNVKMDYVIRGGKRIGIYFLEHGASIRPSK
                     IIYDRSNSAITEIQSGMLDWVTIFKEKEWFHITGITPALSESTARESLEAIKIAKNSG
                     LTVSCDLNFRKKLWTRERAREVMSEIVQYVDVIISNEEDAFDIFGIKAGDSDVYAGKL
                     DIDKYKSVAEQLMAISTAKKVAITLRESISASDNNWSAILYDGTDFYQSRKYSIHLVD
                     RVGGGDSFGAGLIHGIVHGWDMPKVVEFAVAASALKQTIPGDMNLVKEDEVLAIVKGD
                     ISGRVQR"
     gene            5540..6013
                     /locus_tag="PLI55_06995"
     CDS             5540..6013
                     /locus_tag="PLI55_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00619.1"
                     /translation="MLITVNVNFKRFYLKIAILIFFFGAIINAETILSGDFESFVIGS
                     LNGQNGWAVNSGNCVISDDSNNVKSGNRAARFIANNQTLVVQNTSFSGSESGATGVVY
                     VDFWVKINSMAEKDFGISGYDLFGSSQKRSFVLEFGAPSGGSSELSGYLYGSSNW"
     gene            5994..6443
                     /locus_tag="PLI55_07000"
     CDS             5994..6443
                     /locus_tag="PLI55_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00620.1"
                     /translation="MAVAIGNQVPKGIDIRPNHQQGIERQLAILALNHMDGIDTDSFD
                     PQYTKIQTTDYRIEVYFGCANDGLRTTAPQRLKWFEISGKNLIFFQMDADIEKHSVCC
                     QNQNVDCPEVVRYTWADNPECHLLNGFGLPDSHFRTECWHELTGRGK"
     gene            6440..7483
                     /locus_tag="PLI55_07005"
     CDS             6440..7483
                     /locus_tag="PLI55_07005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB00621.1"
                     /translation="MKDLAERLRQENERLKDELSTVRREIENCKQSEKTYRNLFEDAP
                     VGYHELNTDGIILRVNRTEAGMLGYQQNEMIGQPIFDFIYEEHRSVARQFFHEKIKNW
                     RPVIGFSRKYQTHSGREIDVYITDQIVYDDQLNIICIRSILRPISGESNKIGYHEVDS
                     SGIILQVSETEAKLLGYRQNEMIGKPIFDFIAPSEREVARKAFQEKTAHWVPTYGFER
                     RYQHKNGKWVDVMIVDRPVYDDKNIIVGIRSTIQEITEFKKVQQERDQLMNELSDALR
                     RIQTLSGLVPICASCKKIRDDQGYWRDVEAYVAKHSGAEFSHGICPDCMKKLYPEEYA
                     RILSRERKAKTSQ"
     gene            7567..9774
                     /locus_tag="PLI55_07010"
     CDS             7567..9774
                     /locus_tag="PLI55_07010"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HPB00622.1"
                     /translation="MQFTHKKYLYISVLFTGILSMTNLGFSGKIPDDHIPTDKEIFAA
                     LDTSITDVKEAVNLFNCGQIDSAKIVLTEYFKDKSADRYYFSWHKFYERFYEYQILYP
                     NAKKEHFKATKYQMDHFGAETSWILPFKDLTGKEVTAYELRHLARQYRSADMTLMYYY
                     ENENEKYLDYFIRQVKNLNQAFESGQYDNAGNGIYENYRAGLRIHNWLFDHHAYLASQ
                     KYSWEDQWLMIKTFLHHAAQLQKSTRKYSPGNHHTKGLVALFEIAAIFPEFTDSKAWR
                     EQAIKGLTEHLTLEVNSDGFQFERSVHYHIGDIENYLRTILLARRNNIELPQIFNQRV
                     HQMFDALVKIAYPDKTGPVLQDDTDHFLREYNILSEPFTIAYLLYQESVFGYFTSDKV
                     NHSLYWLFNETELSQLNKKVSEPPDILSVDLPETGYYIMRSGWKPDDVVCIITAGLSA
                     IKPDHQHGDMLGIQLYGLGRVLLPNYQVKYDQEDYIDFKNSLVKNVAIADDQLQGQKW
                     QPNEGESGFGKWLRLPQPEVIVWVDHSPEWTYFKGTHNGFIENGIDYQREVFQYRNRW
                     IVVIDRFVSEQVHDYQQIWQGNFQQAQNAIFQKIDADTFFIRQINNCDYTIQTRKLRD
                     KQANIFKMQDKSHTFYTLLDLSNDSKISYKMQNSGCIIIDRNLNFYSDIKTLNYKNRR
                     ISFDKLSSCVILPQSDEVYLIGSENREMTITRKGIKKSKKVILEPNVRWKLNE"
     gene            complement(9957..11183)
                     /locus_tag="PLI55_07015"
     CDS             complement(9957..11183)
                     /locus_tag="PLI55_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00623.1"
                     /translation="MNTNFISKEEKIKILIDIINSSEFQNAQKFQELLRYLVDASISG
                     KIIKESTIAIEFFQKDKSFDPSIDSTVRTSISTLRKKLEHYYLTTGKTNSTKLTIPKG
                     HYNIVFVDSNYSKPKNNLKKKFYLYSLIFVLCLIILITLITNLIPRKNTQIIPYRDPI
                     WNDIFSNSMKTLIVLGDYYFFYLPFENGRHSYIRDVEINSEEDLNDFLKNNPQYSTRI
                     SKTYHTYLDESLPICISKILPSFNHYKLQYELKMSSELQLDDLQKYNIIYIGSYKCLY
                     LLSTITRNLHFQYFQKPGSSTLIYHQQSDSVDYQYTWLINPVTNARNDYAIVVKASGQ
                     NNNIFMMFLSEHDFGNLGTVKYFTDPIKLKELYLKIRSNLFEALFEVTGVKRTDFSIE
                     LLHYNPLHSEYKIKIQ"
     gene            11642..>13079
                     /locus_tag="PLI55_07020"
     CDS             11642..>13079
                     /locus_tag="PLI55_07020"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPB00624.1"
                     /translation="MRANSRIISIFLFIGLLLSTNVFAGETGKIAGTVTDANTGAPLI
                     GANVIISSIWIDGVEHSLDFIQGSASDLEGRYFILNVRPGVYSLKVNFIGYGTQIITQ
                     VVVNVDKTTTVNFELQPQAIEGEAVTVVAQKVHAVESDLTATKQVYQVSDVQSIAGVA
                     DIADIISLQADVVDDHFRGGREGESRYILGGGVIVNPLNNSRAFRPIVTGLEQVEVYT
                     SGFSAEYGNAQSGVINMVTKEGRSEWESRLEFSLTLPHYKSWQEVIDKEGNVSYKGGS
                     PYSANSMDFYSMLDDEEEWLVGYNPVYQLPIYDSGTSFTGIYFPNRVVWPPNPLGHSD
                     SLQLAHLVMTQFMLAMRDIGLEYKNTMDNRLDFSTGGPISDHMRIFIAGRQNTEYCII
                     PTSKPDLNQQIMSSLVYQKNIKNKFKFTFTLDNTNETYFSSSWRYWLLDPTLATSQIS
                     TSSRQYGLEWGHVVNDATFLEMKLNQLNL"
BASE COUNT         4144 a         2028 c         2816 g         4091 t
ORIGIN      
        1 ataatttttt catgcggaca aaaccaggct tcagaacacc cttcgcttgt aattagtgct
       61 gaagaaggac gtgaaattca tgcgactttg ggaaaatatc caattctcga taaatcttat
      121 aaaaaaattg ttgatgaagt tactgtagca ttagctaagc cggttgatgt cccccgacct
      181 ggtgaagctg gtggatatga gcatgagagg cacaaacaaa attatcagga gatgaaatcg
      241 gcaggtatat tatttactat tacgggtgat gaaaaatatg cacggtttat caaaaatata
      301 cttgataagt atgcagaact atatccgacc ttagggccac accctttgtc ccatgatcaa
      361 aaacctggta agttgtttca ccaaatgctt aatgaaacag tctggttgct aaatacagct
      421 attgcatacg attgtattta taattggctg aacaacgaag atcgacagaa atatgaagaa
      481 aatattttta caccaatggt aaaatggttt actacagaac atccagctga gtttaatcgc
      541 attcataatc atggaatgtg ggcagcagct tcggtgggga tgattgggta tgtaatcaaa
      601 aatcaagatt atattgatat ggcgttatat gggaccaata aagatggtac aggtggtttt
      661 ctaaagcaaa tagagatgtt gtattctcca gatggttatt atttagaggg accatattat
      721 agccgttatg ctatggaacc attagtatat tttagcgaag caattgagcg caatcaacca
      781 aaactgaaaa tatttgagta taaggataag attataaaaa aagcatatta tgcggcggtt
      841 caatcaacat tccccaatgg cgtttttttc ccgatcaatg atgcatccag aacaatgaat
      901 attctggcgc cggggataat atatgggaat agcctagttt tttttagata tgggacagat
      961 attaatttac ttggtattgc caagatacaa caagaagttc cgcttaacgc cttaggtttg
     1021 aagctggcac aagagtataa atccttaaag gaagtacctg atttcagctg gaaaagctgc
     1081 gaatttttag atggctttga tggtaaacag ggtggactag gaattctacg ccatggtgaa
     1141 ggagcagatc agacggtcct tattatgaaa tacggagtac atgggtttgg acatgggcat
     1201 tttgatatgt tacattttat gttttataat caacaacgcg aagtaatttt tgattatgga
     1261 tatgcacgct ggataaatat tgagcccaaa ttcggtggcc gttatttacc tgaaaataaa
     1321 agttatgcaa tgcagactat tgcccataat acagtagtag tggatgagac tacccaactg
     1381 aaattcaatc aaaaacaagc agatcaaatg catgggaatc ggcatttttt tgatgctgaa
     1441 aatgagaata ttcaggtaat gagtgccaaa gccaatgatt actatccagg tatcaaaatg
     1501 caaaggacga tgtttttaat aaaagatgtg cacttggagt atcctgtcgt tgtagatatt
     1561 tatcgtctct caagttctca gactcatcag tatgactatc caatccacta tcatggacag
     1621 ttaatcaata ccaattttaa atatacagcc agtactaagg ttcagaaagc tatgggtgat
     1681 aattatggtt atcagcatat ctggagtgaa gcttatgccg ttgttaatca atgcccttcg
     1741 attacctggc tagatggtaa tagatattac acacagttga catcctattt acctaaaacg
     1801 gaggtatatt tcggtcgtat cggcgctgaa gacccggatt ttaatttaat ctccgaacct
     1861 atgacgatta ttcgtcaaag tgggaaggac catctttttg cttcaattat tgaaccgcat
     1921 gggtattttg atgagtctcg agaaatatcc tatcaagctc gaccgatttt taaacaaatt
     1981 agggttgttg gtttcaatga taaaggcagt gtaatcgagg ttttaggtga gggagacata
     2041 cactggaggt taatggtaaa taatttggat gcatctgata aaaaggagca tatattagaa
     2101 tttaatggtg aaacgtatcg atgggtagga aattatcgtg tcgatctgga tgtaaaataa
     2161 aaatttataa tggcaggagt acttatgaaa aaagttaaag gattacgctg gtggattata
     2221 ggtatgattg cactagcaac cgtgatcaat tatattgatc gaggcacact ggcgataatg
     2281 tggccgagta tttccaaaga ccttggaatg acaaaacaaa attatgcatt tattttaaat
     2341 atgtttatga ttgcttatgc gattgggcag acggtttctg gtagtttatt tgataaagtt
     2401 gggactcgca tcgggtatgt tatcacgata gcggtttggg gggtttcttc gtttttacat
     2461 tccattgcgc gtagtatttt ttcctttagt ttttttagaa taacgctggg attaggcgaa
     2521 gctggtaact ggcctggggc ggtcaaaagc aatgcagaat ggtttccggt aaaagaacga
     2581 gcgattgccc aaggaatttt caattcgggt gcttcaatcg gttcagttat cgctccacca
     2641 ttgattgcta cgctctgggc ggcgtttggc tggaaggtta cgtttatgat tgttggatgt
     2701 tttggattaa tttggattat accatggtta atcattaata aaagcggacc ggaaaagcat
     2761 ccctggatta cagaagcaga gcgagaatac attctttcag gtcaacctag cgttaagaat
     2821 agtaggggtg aagaaaaagg gttgaaagcc aaagagattt tgagtcataa agaggcttgg
     2881 tcagttttag tttcgcgatt cttcctagaa ccggtttggt ggctatttgt cggttggatg
     2941 ccgatttact tagccgataa gtatggtttt aatgtccaac aaattggtta ttttgcatgg
     3001 gttccttatg tcggtgctgc attggggagt attattggtg gtggatatgc tggtagattg
     3061 atgggtcgag gtaaaactgt caattcagcg cgcaaacagg caattacaat tggtggagtc
     3121 attatgtttc ttggtttatt agcgacgata ttttttgcca atacaccatt gagattcgta
     3181 attattgtag cgtttgttct atttggattt caatttacga ttggaaatgt tcaaactatc
     3241 ccgagtgatc tgtttagtgg taaatcagta ggcaccttag ctggaatcgg gggtagtgtt
     3301 gtagtatttt cagttattat tatgaatttt ttggtaccga ttatttccaa aatatcctat
     3361 ataccaattt ttataatgat tgcggcactt gtaccccttg gagtagccgc tgtttattta
     3421 ctagtcaaag aaataaaacc acttaatttt aaatagacaa ttagagaggt aagtaatgaa
     3481 aacgcatata aaaaatagtg atgtaccaat tgaagaggta gcctggggaa tgactcgcca
     3541 agttatggga tatgatacga atttgatgct tgttagagta tgttttaaaa aagatgctgt
     3601 tgggtctgaa catgctcata tccatcagca ggttacttat attgaaaagg gtatttttga
     3661 agttaatatt ggcgggaaaa aggaggtatt aaaagcaggg gatgcttatg ttattccatc
     3721 gaatgtacta catggggcag tatgtttaga agatggtata ttgattgaca ccttttcacc
     3781 aatgcgtgag gattttataa aaaagtaaat agtgagggga agtatgaatc gacaaaagat
     3841 tttagaccga cttaaagact caggtatcat tgctgttatt cgcttagatg gtgtcgaaaa
     3901 cctagataag ataattgatg ctctagcaaa gggtggggta caagcacttg aggtgacgat
     3961 gactacgcct aatgctgtag caatcatccg cgatatttct ttttctcttt ctgaagattt
     4021 tcttattgga gccgggacgg tattaaatac tgatacagcc aaagaagtaa ttgacgccgg
     4081 ggcgcaattc gttgttagcc cagtatttaa gccttcgatt attgaaattg ttcatcaata
     4141 tgataaagtt gctatttcgg gagcgttcag cccaaccgaa atactgtctg cctgggagac
     4201 aggtgcggat atagtgaaga tatttccagc tactgtgcta gggccagtat attttaaaga
     4261 tattcatgga cctctgccac aagtaaagct aacccctact ggtggcgtta atcttgataa
     4321 tgcggaggag tttattcgtt gcggagctgt ttgccttgga gttgggacag ccttattaga
     4381 taagaaattg ataaaaaacc aggattggga tgctctggag gaaaaggccc gtcaattcaa
     4441 agctgcggtt gaacgagggc gaaaaggata aaaaatgact gctaaaatcg ttacattcgg
     4501 cgaaattatg cttcgtttag ctccatttaa tttcgaacgt attgttcaag cgaaccaatt
     4561 tcaagtaact tatggaggtg gtgaagccaa cgtggcagtc tcattggctc aattaggaga
     4621 tgaatcatat tttgttactc gacttcctga taatccgcta ggagaggccg cctggaaaac
     4681 tttatgcgga tttaatgtca aaatggatta tgttattaga ggcgggaaac gaataggtat
     4741 ctatttccta gaacatggag cttccattag accatctaaa attatttatg accgctctaa
     4801 ttcagccatt acagaaattc aatctggaat gttagattgg gtaactattt ttaaagagaa
     4861 agaatggttt cacataaccg gaattacccc agctctttca gaatcaaccg ccagagagag
     4921 tcttgaagct ataaaaattg ctaagaattc tggcttaact gttagttgtg atttgaattt
     4981 tcgtaaaaag ctctggacac gggaacgtgc ccgggaagtt atgtcggaaa tagtacaata
     5041 tgttgatgta attatttcca atgaagaaga cgcttttgat attttcggga taaaagctgg
     5101 cgatagtgat gtttatgcgg gtaaattgga tattgataaa tacaaatcag tggcggaaca
     5161 attaatggct atatccactg ccaaaaaggt agccataact cttcgggaaa gtatttcggc
     5221 ttcggataat aattggtcgg caatattata tgatggcacg gatttttatc aaagtcgcaa
     5281 atattcaatc caccttgttg atcgcgtcgg tggcggtgat tcttttggtg ctggattgat
     5341 tcatggcata gttcatggct gggatatgcc taaagtagta gaatttgcgg tagcagcttc
     5401 ggccttaaaa caaacaattc ccggtgatat gaacttggtt aaagaggatg aagttttagc
     5461 gattgtcaaa ggtgatatat cgggtcgggt tcagcgctga gtctaatata gacttatagt
     5521 tattaagcaa aatagaatta tgctaataac tgttaatgtc aactttaagc gtttttactt
     5581 aaaaatagcg attctcatat tttttttcgg agctataatt aacgctgaaa ctattctaag
     5641 tggagatttt gaaagctttg ttataggatc gctaaacggt caaaatggct gggccgtaaa
     5701 tagtggtaat tgtgttatca gtgacgattc caataatgtt aaatccggta atagagcggc
     5761 tcgttttata gcgaataatc aaaccctagt tgttcagaat acctctttta gcggatcaga
     5821 atccggagca actggggttg tttacgttga tttttgggta aaaatcaatt ctatggcgga
     5881 aaaggatttt gggattagcg gatacgatct tttcggaagc agtcaaaaaa ggtcatttgt
     5941 cctagaattc ggtgcacctt ccggtggcag tagtgaattg tcaggatacc tatatggcag
     6001 tagcaattgg taatcaggtt ccgaaaggca ttgatattcg tccaaatcat caacagggaa
     6061 tcgaacgaca gctggcaata ttggcattga atcatatgga tggaattgat actgatagtt
     6121 tcgatccgca gtacactaaa atccagacaa cagattacag aattgaagtc tattttggat
     6181 gtgctaatga tggtctgaga acaacagccc cccaaagatt aaaatggttt gaaatttcag
     6241 ggaagaacct tatctttttt cagatggatg cggatattga aaaacattca gtttgttgcc
     6301 aaaatcaaaa tgttgattgc ccagaagtcg ttcgctatac ctgggcagac aatcctgaat
     6361 gccatctttt aaacggattc gggttaccag atagtcactt tcggaccgag tgctggcatg
     6421 aattaaccgg gaggggaaaa tgaaagattt agcggaacga cttcgtcaag aaaatgaacg
     6481 cctgaaagat gaattatcaa cagtccggcg agagatagag aattgtaaac agtcggagaa
     6541 aacctaccgg aatctttttg aagatgcgcc ggttggatac catgaattga ataccgatgg
     6601 aatcatcctg cgagtgaatc gaaccgaggc gggaatgtta ggctatcagc agaatgagat
     6661 gattggccaa ccaatcttcg attttatcta cgaagagcat cggtctgtag cccgtcagtt
     6721 tttccatgaa aaaatcaaaa actggcgtcc cgtcatcggc ttttcccgaa aatatcagac
     6781 ccacagtggc cgagagattg atgtctatat caccgaccaa atagtctatg atgatcagtt
     6841 gaatattata tgtatccgat ctattctgcg ccctatttcc ggggaatcta ataagatagg
     6901 ataccatgaa gttgattcct cagggattat tttacaggtt agcgaaactg aagcgaaact
     6961 acttgggtat cgccaaaacg aaatgattgg taagccgatt tttgatttta ttgccccatc
     7021 tgaacgggaa gtcgcccgta aagcattcca ggaaaaaact gctcactggg ttccaaccta
     7081 tggatttgag cgccggtatc aacataaaaa cggtaaatgg gttgacgtta tgattgttga
     7141 tcggccggta tatgatgata aaaatataat tgtcggcatt cgttcgacga ttcaagagat
     7201 tactgaattt aaaaaagtcc aacaagagcg ggatcaatta atgaatgaac taagtgatgc
     7261 tctccgtcgc atccaaaccc tgagtggatt ggtgcctatc tgtgcttctt gcaaaaaaat
     7321 ccgggatgat cagggctatt ggagagatgt ggaagcgtat gttgccaaac attcgggcgc
     7381 agaatttagt catggtattt gtccggattg tatgaaaaaa ctttatcccg aagagtatgc
     7441 ccggatatta agccgggagc ggaaggcgaa aacctcccaa tgaaaacctt gttatcctat
     7501 ttgtcagtcg gtccccttta taaaaacaat tggctggatt taactacaaa taaaaagtga
     7561 gaatttatgc aatttacaca taaaaaatat ttgtacatta gtgtcttatt taccggaata
     7621 ttatcgatga ctaatttagg attctctgga aaaataccag atgatcatat tcctacagac
     7681 aaagaaatat ttgcggctct tgatacttcc ataacagatg ttaaagaagc agtcaattta
     7741 tttaattgtg gtcagataga ttctgctaaa attgtcctaa ccgaatactt taaggataaa
     7801 tctgcagatc gatactactt tagctggcac aagttttacg aacgatttta tgaatatcag
     7861 atcctttacc cgaatgctaa gaaagaacac tttaaagcca caaagtatca aatggatcat
     7921 ttcggggcgg aaacatcgtg gatattaccc tttaaagatt taactggtaa agaagttacc
     7981 gcatatgaac taagacactt ggcgcggcaa tatcgatcgg cagatatgac gctgatgtat
     8041 tattatgaaa atgaaaatga aaaatatttg gactatttta tacgtcaggt aaagaatttg
     8101 aatcaagcat ttgaatctgg ccagtatgac aatgctggca atggtattta tgaaaattac
     8161 cgggctggat tgcgaatcca taattggctg tttgatcatc atgcctattt agccagtcaa
     8221 aaatatagct gggaagatca gtggttaatg atcaaaactt ttttgcatca tgcggctcaa
     8281 ttacagaaaa gtacccggaa atattcgcct ggaaatcacc ataccaaagg tctagtagca
     8341 ttatttgaaa ttgcggctat ttttccagag tttacggatt caaaagcctg gcgggaacaa
     8401 gcgattaagg ggttgacaga acacttgaca ctggaagtaa attcagatgg gtttcaattt
     8461 gaaaggtcag ttcattatca cattggagat atcgaaaact atttacggac cattttgctc
     8521 gctcgtcgta ataacattga gttgcctcag atttttaatc agcgcgttca ccagatgttt
     8581 gatgcccttg tgaaaattgc ttaccccgat aagaccggtc cggtattaca ggatgatact
     8641 gatcattttc taagagaata taatattctg tcggaaccat ttacaatcgc atatttactg
     8701 tatcaagaat cggtgtttgg ctattttacc agcgataaag taaatcactc tttatattgg
     8761 ttatttaacg aaaccgaact ttctcaacta aataaaaagg tgtcagaacc acccgatatt
     8821 ttatcggtag atttaccgga gacagggtat tatataatgc gttcaggctg gaaaccggac
     8881 gatgttgttt gtattatcac ggctgggtta tcagctataa aacctgatca tcaacatggt
     8941 gatatgcttg gaattcaatt atatggatta ggaagagtgc tattaccaaa ttaccaggta
     9001 aaatatgacc aggaagatta tattgatttt aaaaatagtc tggtgaaaaa tgttgctatt
     9061 gctgatgatc aactgcaggg gcaaaaatgg caaccgaatg aaggggaatc tggttttggt
     9121 aaatggctga gattgccaca accggaagtt atagtctggg ttgatcattc acctgaatgg
     9181 acttatttta aaggaacgca taatggattt attgaaaatg gtattgatta tcaacgggaa
     9241 gtgtttcaat atcgaaaccg atggatagtc gtaatcgatc gttttgtttc agagcaggtg
     9301 catgattatc agcaaatctg gcagggtaat tttcaacaag cgcaaaatgc gatatttcaa
     9361 aaaattgatg ctgatacatt ttttataaga cagataaata attgcgatta cacaattcag
     9421 acccgaaaat taagggataa gcaagctaat atctttaaaa tgcaagataa atcgcatact
     9481 ttttatacct tgctggactt aagcaatgac tctaaaataa gttataaaat gcaaaattcc
     9541 ggttgtatta taatcgatcg caacctcaat ttttatagcg atataaagac tttgaattat
     9601 aaaaatcgcc gaataagttt tgataaactt tcatcatgcg taattttgcc tcaaagtgat
     9661 gaagtttacc ttataggaag tgaaaataga gaaatgacta ttactcgaaa gggaataaaa
     9721 aaatcaaaaa aagtgatttt agagccgaat gtcagatgga aattaaacga gtaattattg
     9781 tagtttttca ttgttattag atttttatct tatttaaggg cacaacttgc tacattgagg
     9841 tcggattacc atacctataa aatccccaca acttatttaa aggaatttat tttcattata
     9901 agagctcgga taaagattat tcgaaccagt tttaactgaa agctgttttt ccgtttctat
     9961 tgaattttaa tcttgtattc actatgcagg ggattgtaat gaagtaactc gatactgaag
    10021 tcagttcttt taacacccgt aacttcaaac aatgcctcga ataaattaga gcgtattttc
    10081 aaatatagct cttttagttt aattggatcg gtaaagtatt tcacagttcc tagattacca
    10141 aaatcatgct cggaaaggaa catcataaaa atattattgt tttgtcctga tgctttaaca
    10201 actattgcat aatcattacg ggcattagta acaggattta taagccaggt atattgataa
    10261 tcgacactgt cactttgttg atggtaaatc aatgtgctgg aaccgggttt ctgaaagtat
    10321 tggaaatgga gattgcgggt tattgtactt agcaaatata gacacttata tgaaccaatg
    10381 tatattatgt tgtatttttg aagatcatct aattgaagtt ccgaagacat ttttagctca
    10441 tattgtaatt tgtaatggtt aaatgagggt agaatcttag aaatgcagat cggtaagctc
    10501 tcgtcgagat aggtgtgata ggttttagaa atacgagtag aatattgagg attatttttt
    10561 aaaaagtcat ttaaatcctc ttcagaattg atttctacat cccgtatata actgtgtctt
    10621 ccgttttcaa atggtaaata aaagaaatag tagtctccta agacaatcaa tgttttcatc
    10681 gaatttgaaa aaatatcatt ccatatcggg tctctatatg gaataatctg ggtatttttt
    10741 ctgggaatta aattagttat tagagttatt aaaattatta agcataacac aaaaattaat
    10801 gagtaaaggt aaaatttctt tttaagatta tttttaggct tggagtagtt actgtctaca
    10861 aatactatgt tgtaatggcc tttcgggatt gtcaatttag ttgaatttgt ttttcccgtt
    10921 gttaagtaat aatgctccaa tttttttctg agggtactga tgcttgttcg aacggtagaa
    10981 tctatagatg gatcaaaaga tttatccttt tgaaagaatt caatagctat ggttgattct
    11041 ttaataattt tgccactgat ggaagcatca actaaataac ggagtaattc ttggaatttt
    11101 tgggcgttct gaaattcact gctattgatg atatcaatta ggattttaat tttttcttct
    11161 tttgagataa aattggtatt catagaaatc ttaaaaatct aattgtaaat atttagattt
    11221 aatttttgat atgcaagcat ttttatattt gcctgtagat tacatgttat ctactgttat
    11281 taacccaaaa atacttaaaa aaattaaacc tatttgatat tattagtgag cgtagtgata
    11341 gaattatggt gagtaaaaaa agagaatgac ttagttttga aaatgcaaaa aaatacttga
    11401 caaatcatat aagtcttatt aacttgtcat acatgttaca aatgagtata aaatatcaca
    11461 agcatatctt gaaaaatgat gaccttgttt aatacatttg ggtaataaat aaagtgggta
    11521 tttttcctaa atataggctt gtttttaaca tcttgcgggt tttggaaata gttgatatgt
    11581 tagaaaaatt aaggtattta atctcgaaaa tattagtagg agttttttgg gaggaatttg
    11641 aatgagggct aattcacgga taatttcaat ttttttattt ataggattgt tactatctac
    11701 taatgttttt gctggtgaaa ctgggaaaat tgctgggaca gttaccgatg caaatacagg
    11761 ggcaccttta attggggcca acgttatcat aagttcaatc tggatagacg gagtagaaca
    11821 tagcctcgat tttattcagg ggagtgcgtc tgacctagag ggacgttatt ttattcttaa
    11881 tgtgaggccg ggagtttatt cattgaaggt gaattttatt ggttatggta cacaaatcat
    11941 aactcaggtg gtcgttaatg ttgataaaac cacgacggtg aatttcgaat tacagcctca
    12001 agcgatagaa ggagaagcgg taacggtcgt cgctcaaaaa gttcacgcag ttgaaagtga
    12061 cctgacagcg accaaacagg tatatcaggt gagtgatgtt caaagtattg ctggagttgc
    12121 ggatattgct gatattatat cacttcaagc agatgtagtg gacgatcatt ttcgtggcgg
    12181 tcgcgaaggt gaatccagat atatcctagg tggcggtgta atcgtaaatc cacttaataa
    12241 ttctcgcgcc tttcgcccga ttgttaccgg tctagaacag gtagaagtct atactagcgg
    12301 ttttagtgcg gaatacggta acgcccaatc tggggtaatt aatatggtga ctaaagaggg
    12361 gcgaagtgaa tgggaaagtc gtttagagtt ttctttaacc cttcctcatt acaagtcttg
    12421 gcaagaagta attgataaag aaggtaatgt aagctataaa ggtggaagtc cttattctgc
    12481 caatagcatg gatttttatt caatgttaga tgatgaagag gaatggttag taggctataa
    12541 tccggtttac caacttccaa tatatgattc tggtaccagt tttacgggga tatattttcc
    12601 caatagagtt gtatggccgc cgaatccgtt agggcacagt gattctctcc agttagctca
    12661 tttagtaatg acgcaattta tgttagctat gagggatata gggcttgagt ataaaaatac
    12721 aatggataac cgtttagatt tttctacagg tggtcctatt tctgatcata tgcggatttt
    12781 tattgctggt cgtcagaata cagaatattg tattattcca acaagtaagc ctgatttaaa
    12841 tcaacagatt atgagcagtt tagtttacca aaaaaatatt aaaaacaaat ttaagtttac
    12901 ttttacttta gataatacta atgagacgta ttttagtagt tcctggcgtt actggctact
    12961 agatccgacc ttagcaactt cccaaatctc aacaagttcc agacaatatg gacttgaatg
    13021 ggggcatgta gttaatgacg ctacattttt ggaaatgaaa ctgaatcaat tgaatttat
//
LOCUS       DAOOMU010000064        13057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_5268_length_13057_cov_24.347946, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000064 DAOOMU010000000
VERSION     DAOOMU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5268_length_13057_cov_24.347946"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PLI55_07025"
     CDS             complement(<1..1283)
                     /locus_tag="PLI55_07025"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPB00625.1"
                     /translation="MNFTFSIYYVPLILSIIICAVIMFHSWRLRRDSHGIAFFFLALG
                     CIIWAFCNIMEYGFNTLSTKTFWVSIEYIGIEMVIVAWFIVIANFTGQTKFTTRRSIL
                     LLCIIPIITVFLIWTNRYHGLMRRNITLDTSGPFSIIKKSYGIWFWVTFAYSNLLMIS
                     SSIMLMQRLIQPPKLRRSQILALMACALFPWVGNFLYVFNLLPAPWSRLDMTSTLLSV
                     SGAIMAFGLFRLQIMEIVPIARDCILENLTDAILVIDKRNRVADYNPACRNMLKLNEI
                     IIGQPVEEVLNPLAIRPELIAEIKNVQEEISFAFGNETKIYDLRILSLFKKKQKLCGR
                     IFHFRDVTDKRLAEMHLEKTVVDLRRALTEVRTLSGLLPICSSCKKIRDDTGYWKDVE
                     TYISQHSTAEFSHGICPDCMQKLYPEQYETLVKKG"
     gene            complement(1429..3060)
                     /locus_tag="PLI55_07030"
     CDS             complement(1429..3060)
                     /locus_tag="PLI55_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00626.1"
                     /translation="MFVNFNSVTTIPAIPDWWFWLWPALMYAEMHYRLKFLRGRLFKR
                     QPEILFDVPHRIQTTRLPVVLIIKDAHWFPVTLKQVTLKITSPFDDNLCVEQVFSENL
                     KVDEPWFTREYSLEVAKFRNHLLSVNCTAILDCDGRIITIQNDNYAGLSQADFSVYVD
                     SEPLPADSGWIWGDLHCHSQWTSDQVEFGVPLAVLPDLAKAMGLSFCGLVEHSYDLDD
                     LPDSWTKNDPELRQWFAYRQAVQATNAKLNDFIIIPGEEVSVDNGLGRNVHLAVLNNS
                     EFFPGSGDGLERSVGFASELTYQRVLERAESQALIFAAHPLCKPPLTQQIIARRGIWN
                     LHDQFQRLNGWQILNGQEGKEFSASRNFWIKKLLSGNHSYIYAGNDSHGNFNRFRQVK
                     IPLLLMHENDHQIFGEFLTGVQTDLATGLDDLVANLKNGAVIVSNGPFLNIKAIDSRQ
                     NRYAIGQTMPELPQSVEITALSTRHCGAIHSIRLYIGNFERQVEEIKTVEVAGGIYRN
                     IINLPVSNLPNSGYFRAEVETKKRKFALTNPIWFS"
     gene            3213..3773
                     /gene="efp"
                     /locus_tag="PLI55_07035"
     CDS             3213..3773
                     /gene="efp"
                     /locus_tag="PLI55_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPB00627.1"
                     /translation="MATTADIKNGLVINFNGDLVKVIEFQHIKLGRGGARVWTKFKKI
                     RTGQVIENTFRSGEKIDIVRLEAVEMQFLYRDGSDYVLMNTETFEQITVPEEIFKDAA
                     QFIKENELVKVTFFEGKPIDVEVPIFVNLKVVKTEPGMKGDTVTGASKPAVLETGYTV
                     QVPLFINAGDIIRIDTRSGQYCERVK"
     gene            3789..4271
                     /gene="accB"
                     /locus_tag="PLI55_07040"
     CDS             3789..4271
                     /gene="accB"
                     /locus_tag="PLI55_07040"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HPB00628.1"
                     /translation="MRKEKILELVKILESHEIDEIEISRWGQKIRISKNSNTGISTGV
                     EVAPAPQKAKKGHQPQLAETITFESRTAVTEEINHKGVEVKAPIVGTFYRSPAPDAEP
                     YVNVGDTVAVGQPLCIIEAMKIMNVIEAEVAGKVTKILVENAQPVEYNQTLFIIEKTE
                     "
     gene            4293..>4549
                     /locus_tag="PLI55_07045"
     CDS             4293..>4549
                     /locus_tag="PLI55_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015809619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HPB00629.1"
                     /translation="MIKKLLIANRGEIALRIIRACKELNIPTVAVYSTADALSLHVRF
                     ADEAVCIGPPESKNSYLKMANIISAAEVTNSDAIHPGYGFLA"
     assembly_gap    4550..4649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4650..4904
                     /locus_tag="PLI55_07050"
     CDS             <4650..4904
                     /locus_tag="PLI55_07050"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013455318.1"
                     /note="catalyzes the ATP-dependent carboxylation of a
                     covalently attached biotin and the transfer of the
                     carboxyl group to pyruvate forming oxaloacetate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HPB00630.1"
                     /translation="GPGTRMDTHVYAGYEIPSYYDSLLGKLITHGKDRDEAIVRMERA
                     LDETIIEGVPTTIPFHKAIIRNEAFRKGDFNINFLNNFSF"
     gene            4921..5298
                     /gene="gcvH"
                     /locus_tag="PLI55_07055"
     CDS             4921..5298
                     /gene="gcvH"
                     /locus_tag="PLI55_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPB00631.1"
                     /translation="MKVPANLKYTKDHEWARIEGAVAVVGITDYAQSQLGDVVYIDLP
                     KVGDVVARGDVIGTIEAVKTVADIYSPVSGEIVAVNSALKDASELVNKDPYGEGWIAS
                     IKLSNSMELEALLDPADYEKLIS"
     gene            5302..6642
                     /gene="gcvPA"
                     /locus_tag="PLI55_07060"
     CDS             5302..6642
                     /gene="gcvPA"
                     /locus_tag="PLI55_07060"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012505128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPB00632.1"
                     /translation="MHFIPNTCDTERAILENLGVASFNDLIANIPEKFREQCRINLPP
                     ALSEIELTKTMQELSEKNGNSDKQVSFLGGGSYDHFIPAVVDFLISRSEFYTAYTPYQ
                     AEVSQGTLQAIYEYQSQICHLTDMEVANASMYDGATALAEAAIMAANITQRKKFLVSP
                     LINPLARQVLETYLTARDFILADLPEKNGQTDLSVLKEMIDNQTAAVLVQSPNFFGII
                     EDLNYIAEIIHAKSSLLIMSVDPISLAILKTPGELGADIAVAEGQPLGIHQSFGGPYL
                     GVFTTRRQYVRNMPGRIVGQTTDVEGKRGYVLVLQTREQHIRREKATSNICTNQGLMA
                     LAATIYLSLMGKTGLRKVAELCLNNSHYLADKIAGLPGYSLPFAGQPFFKEFVVKTPR
                     PATEIAAAALATGFFAGIDLGQYRREWENYLLIAVTEKRNLQEISDFVKILAKF"
     gene            6648..7400
                     /locus_tag="PLI55_07065"
     CDS             6648..7400
                     /locus_tag="PLI55_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HPB00633.1"
                     /translation="MGVLNQLLEKARLNGGGYFVLIDPDTRSTPELKAFVREICACGV
                     DAILVGGSLIIGGGFHETLDIVKTESDVPVIIFPGSVAQVSNRADAVLYISLISGRNP
                     NALFGEHVIAAPVIRKLGIEPISTGYMLFESGKTTTAEFMSNTRPLPINKPEIAMAHA
                     LAAQYLGMSTVYLEAGSGAERSIPNQLVKAVCDYIDIPVFVGGGIRTPDEARAKVEAG
                     ATFIVTGNVFDNDANIGLAKEFVRAVHVKEHF"
     gene            7413..7988
                     /locus_tag="PLI55_07070"
     CDS             7413..7988
                     /locus_tag="PLI55_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HPB00634.1"
                     /translation="MHQKIIAQLEESAEVKRQTIITAVDSIEAAAQMLIDCYRAGGKV
                     LLCGNGGSAADAQHLAAELISRLKLERAAIPALALTTNTSLLTAIGNDYKYDLVFVRQ
                     VEAFGRAGDVLIAISTSGESENVIKAVEFSRVQGIKSIALTGAKGGRLAEKVDLAIKI
                     PSDNVQRIQECHITVGHILCELIESALYGTI"
     gene            8004..>8271
                     /locus_tag="PLI55_07075"
     CDS             8004..>8271
                     /locus_tag="PLI55_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPB00635.1"
                     /translation="MISEIQDFINYLKVERNLAPNTIQAYSTDLERYSQFLTQQQIND
                     ISKVRFSDIQNYINVLAELGLAARSLARNYSSIRAFHRFLFSERI"
     assembly_gap    8272..8371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8372..8642
                     /locus_tag="PLI55_07080"
     CDS             <8372..8642
                     /locus_tag="PLI55_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPB00636.1"
                     /translation="LFLSMRGAPLTRMAVWNIIQEYVRLADIRKPISPHVFRHSFATH
                     LLEGGANLRAVQEMLGHADIATTQIYTHLDREFLIEQHRTFHPRY"
     gene            8642..9277
                     /locus_tag="PLI55_07085"
     CDS             8642..9277
                     /locus_tag="PLI55_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPB00637.1"
                     /translation="MNFQVIILLIPPILLALTFHEYMHGWTAYKMGDPTAKMAGRLTM
                     NPIAHLDPIGTLMLFLVHFGWARPVPVDPRYFRNPKRDMLIVAAAGPLANMFIALLSG
                     LLARLFQGGYFSFLPLSVLQPLYVMVILSLQINLGLAIFNLLPIPPLDGSKILMGLVP
                     PRYEAQFNWLERYGSFILMAVIIVGMVTNFSILGAFINPFVNFFSQLFGGI"
     gene            9517..>10555
                     /gene="ftcD"
                     /locus_tag="PLI55_07090"
     CDS             9517..>10555
                     /gene="ftcD"
                     /locus_tag="PLI55_07090"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPB00638.1"
                     /translation="MTEIIECVPNFSEGRDHDVIRAIAVAIEKVKDVKLLDVNSGASV
                     NRTVITFVGNRESVINGAFAGIARAAELIDMRHHRGTHPRLGATDVCPLVPVSGISVE
                     ECIALSHQLAKRVAEELHIPVYLYAKSAQKPERIDLANIRAGEYEGLKQKLSDPNWQP
                     DYGQPIFNPKSGATIIGVRNFLIAFNVNLDTRDCQLAKDIALTIREKGRIKRDQNGNK
                     MYNSQGEPIYETGLLKNVKAVGWYIDEYKMAQVSTNLTNYKVTPPHVVFEEVRRLANA
                     KGLRVAGSELVGLIPKEALLMAGRYYLKKQSRSEDVPESELIQMAVQSLGLNAIAPFD
                     PAKKIIEYRLAE"
     assembly_gap    10556..10655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10656..12494
                     /gene="mutL"
                     /locus_tag="PLI55_07095"
     CDS             <10656..12494
                     /gene="mutL"
                     /locus_tag="PLI55_07095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPB00639.1"
                     /translation="NEWISELMSKIKILSENLTNKIAAGEVVQRPASIVKELVENSID
                     AGADEIIVVIRNGGKTLCEVVDNGEGMNRDDLLLAFERYATSKIATVEDLTNIHTLGF
                     RGEALASIAAVAIVTAISTLRGANEGYELLIEGGTFRDIRPTSPKPGTTISVRNLFYN
                     IPARRKFLRSAEVEFRHIVDVMQEFALIHHDLRFIFIHNDREIYNLSPSSLAERIANL
                     FAPEYSQNLLPVESVADDLKVTGYIGNLNLVRARRGEQFLYINRRFVNDRLMNHAIIS
                     GYGGLLARSEFPFYCLNLELPPEQIDVNVHPTKMDVKFREQDRIYQFLKQATANSLRS
                     ITNVIPDLGTFAPHHYYTTPPPPKGTQIQIPAPDQSPSKQSEFDSSRKTFDVPEVEIP
                     PTPFAAGDSTKWQERARRFVEQGETSEADDFRPSVAVYQVHNKYIISQVKSGLVIIDQ
                     HVAHERILFEEAQESMRGENWKAQQLLFPVVVELTVNDFSTLLEILPFLEKLGFRMRE
                     FGKQTVAVEAVPAGLRWGNENIIIREILDHYQEFGTKDTSIQDKVAASYACKAAIKSG
                     DPLTTEEMQNLVDRLFATSNPYFCPHGRPIIVNLSLKELDKRFERA"
     gene            12498..>13057
                     /gene="miaA"
                     /locus_tag="PLI55_07100"
     CDS             12498..>13057
                     /gene="miaA"
                     /locus_tag="PLI55_07100"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPB00640.1"
                     /translation="MSANSNSINEALSRKIPFIVGPTAVGKTATAIELAKQLNGEIIS
                     VDSRQVYRGLDVGTAKPTLRQQQEIPHHLVDIVDLDEQISAGAYRNLALKTVSEIQSR
                     GRLPIFVGGSGLYVNAVLKGIFQESTTNPEIRREIRQELQEKGVVALYNQLLEIDPDT
                     VLKIHINDVKRITRALEIYRITGQPP"
BASE COUNT         3580 a         2764 c         2962 g         3451 t
ORIGIN      
        1 ttaccctttt ttaccaaggt ttcatactgt tccgggtaaa gtttctgcat acaatctggg
       61 caaattccgt gactgaactc agccgttgaa tgttggctaa tataggtttc tacatctttc
      121 caatagccgg tgtcgtcgcg gattttttta caggacgaac agatcggaag caagccagat
      181 agggttctga cttcggttag cgcccggcgt aagtcaacga cggttttttc caaatgcatt
      241 tcagctagtc gcttgtcggt aacgtcgcga aaatggaata tacgaccaca tagtttttgc
      301 ttctttttga aaagcgatag gatgcgcaga tcgtagatct tggtttcatt gccaaaggca
      361 aaagaaattt cttcctgaac gttttttatt tcagcaatta attcgggccg aatagctaat
      421 ggatttagga cttcttcaac cggttgcccg attatgattt cgtttaactt taacatattc
      481 cgacaagccg gattgtaatc ggctacccgg ttgcgcttgt ctatgaccaa aatagcgtca
      541 gttaagttct ccagaatgca atcacgggcg attgggacaa tttccattat ttgtaatcga
      601 aatagcccga aagccattat agcccctgaa actgatagta aagtcgaggt catatcaagt
      661 cgcgaccagg gtgctggcaa tagattgaaa acataaagaa aattgccgac ccatggaaat
      721 aaggcgcaag ccatcagcgc caggatttgc gaacgacgca gtttgggcgg ttggatcaga
      781 cgttgcatca gcattatcga gctggaaatc atcaacaaat tgctataggc gaaagtaacc
      841 cagaaccaaa tgccatagga tttttttatt atagaaaatg gaccgctggt gtccagagtg
      901 atattccggc gcattaagcc gtgataacga tttgtccata ttagaaatac agtgataatc
      961 ggaattatac ataataaaag aattgaccga cgggtcgtaa acttagtttg accggtaaag
     1021 ttggctatta caatgaacca ggctacgata accatttcaa tgccgatata ttcgattgat
     1081 acccaaaacg tcttggtgct tagggtatta aaaccgtatt ccattatatt gcaaaatgcc
     1141 catataatac accctaaagc caaaaagaaa aacgcaatac catgactatc acggcgaagc
     1201 cgccacgaat ggaacattat tactgcacag ataataatgg acaaaatcag aggcacataa
     1261 taaatgctaa aggtaaaatt cataagattc tcgctgattt aaaagatttt gcttccaggt
     1321 gaatttgaaa acgcctatgc acttttaatg tggctcttaa aatcactgga gatattaaac
     1381 tgttcaaatt gataaaactt tttcgttatt ccaatttcgc ttaaagtgct agctgaacca
     1441 gattggatta gttagagcga attttctttt tttagtttca acttccgccc gaaaataacc
     1501 gctgtttggt agatttgaca ccggtaagtt tattatattt ctataaatac cgccagcaac
     1561 ttctacggtc ttaatttctt ccacttggcg ctcaaaatta ccgatgtaca atcggataga
     1621 atggatggct ccacaatgac gagttgatag agcggtgatt tcaacgctct gcggtaattc
     1681 tggcatagtt tgtccgatcg catatcggtt ttgccgcgaa tcgatagcct taatattcag
     1741 aaaaggacca ttggagacga ttacggctcc gtttttcaaa ttggcgacca gatcgtcgag
     1801 tccggtggca agatcggttt gaacgccagt taagaactca ccaaatatct ggtgatcgtt
     1861 ttcgtgcatt aaaagcagtg gaattttcac ttgtcgaaag cgattgaagt tgccatggga
     1921 gtcattgcca gcatagatat aactatgatt gccggaaagc aattttttta tccagaaatt
     1981 acgactggcg ctaaattcct ttccctcctg accattcaga atttgccaac cattcaggcg
     2041 ttggaactgg tcgtggagat tccaaatacc gcgccgggca atgatttgtt gagtgagagg
     2101 cggtttacag agcgggtgag ccgcaaaaat taacgcttga gattcggctc gctcgagtac
     2161 ccgctgataa gtcagttccg aggcgaaacc gactgaacgc tccagtccat cacccgagcc
     2221 ggggaaaaat tcagagttat ttaagactgc caggtggaca ttgcgtccca agccattatc
     2281 tactgagact tcttcgccgg gaatgatgat gaagtcatta agtttggcat tggtggcttg
     2341 aacggcttgg cgataagcga accattgacg taattcggga tcattttttg tccaggaatc
     2401 cggtaaatcg tccagatcat aagaatgctc aaccaaccca cagaacgata gccccattgc
     2461 ttttgccaga tcaggcagta cagctaatgg tacaccaaat tcaacctggt cggaagtcca
     2521 ttgtgaatga caatgcaaat cgccccaaat ccagccggaa tcagctggca atggttctga
     2581 atcaacataa acgctgaaat ccgcttgact gagaccggcg tagttatcgt tttgaatagt
     2641 aataattcgg ccgtcgcaat ccaaaattgc cgtacaatta acgctcaata gatgatttcg
     2701 gaatttcgct acctccagac tatattcgcg cgtaaaccac ggttcatcta ctttgagatt
     2761 ttcactaaaa acttgctcaa cgcataagtt atcgtcgaaa ggagaggtaa ttttgagggt
     2821 aacctgtttc aaagtaaccg gaaaccaatg agcgtctttg attattagaa cgaccggcag
     2881 tcgagtagtt tgtatccgat gaggtacatc aaagaggatt tccggttgtc ttttaaataa
     2941 ccttccccgt agaaatttta accggtaatg catttcggca tacattagtg ccggccagag
     3001 ccagaaccac caatccggta ttgctggaat cgtagtaacc gaattgaaat tgacaaacat
     3061 agtggtaaaa tctatgattt attcgtattt taatgaagaa aaattccgtt gagaaaggtt
     3121 tgaatagtct atattaattg gaaaaatctt tgaaaataat tcagcgtgcc tgtaaattcc
     3181 ttatcacttt tgatttttaa aatggagtac gcttggctac aacagcagac atcaagaatg
     3241 gcttggtgat aaattttaac ggcgatctgg taaaggttat tgaatttcag catattaaac
     3301 tgggacgagg tggtgcgcgg gtctggacca aattcaaaaa aatccgtacc ggacaagtta
     3361 ttgaaaacac ctttcgctcc ggcgagaaaa ttgacattgt gcgtttagaa gccgtcgaaa
     3421 tgcagtttct atatcgcgac ggttcagact atgttttgat gaataccgaa acattcgagc
     3481 agattaccgt gcccgaggag atattcaaag atgccgccca atttatcaag gaaaacgaat
     3541 tggtcaaggt gacttttttt gaagggaaac caattgacgt cgaagtgccg atttttgtta
     3601 atctcaaggt tgtgaaaacc gagcctggta tgaaaggcga cacagttacc ggcgcctcta
     3661 agccagccgt tctcgaaacg ggctatacag tacaggttcc actgtttatc aatgccggag
     3721 atattattcg gattgataca cggagcggtc agtactgcga acgcgttaaa taaaaacccg
     3781 gaggggtaat gaggaaagaa aaaattctcg aattagtgaa gattctcgaa tcgcacgaaa
     3841 tcgacgaaat cgaaatttcg cgttggggtc agaaaatccg cattagtaaa aatagtaata
     3901 ccggaatatc taccggagtg gaagtagctc cggcgcccca gaaagctaaa aagggtcatc
     3961 agccacagtt ggcggaaaca atcacttttg aatctcggac ggctgttacg gaagaaatca
     4021 accacaaggg agttgaggtt aaagcgccta tagtcgggac tttttatcgt tcaccagcgc
     4081 cggatgcgga accgtatgta aatgtaggag ataccgtggc tgttggacaa ccgctttgca
     4141 ttattgaggc tatgaaaata atgaatgtga ttgaagccga agtcgccgga aaagtgacca
     4201 agattctcgt tgaaaatgcc cagccggttg aatataatca aacccttttt ataatcgaaa
     4261 aaaccgaatg acatttttac gatagaattt taatgattaa aaaacttctg attgccaatc
     4321 ggggcgaaat agctttaaga ataatccggg cttgtaagga attgaatatc ccgacggtgg
     4381 cagtatattc gaccgcggac gctctttctc tgcacgtgcg atttgccgat gaagcggttt
     4441 gcatcgggcc gcctgaaagt aagaatagct atttaaaaat ggctaacatc atcagtgccg
     4501 cggaagttac taactccgat gcgattcatc cgggatatgg ttttctggcn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gtcccggaac gcgaatggat actcacgttt
     4681 atgccggtta cgaaattcct tcctattatg attctctttt aggtaaatta attacgcatg
     4741 gaaaagatcg ggatgaggca attgtcagaa tggaacgcgc cttagatgaa acaattatag
     4801 aaggcgttcc caccaccatt cctttccata aggctataat caggaatgag gcttttcgaa
     4861 agggcgattt taatattaat tttttaaata atttttcgtt ttaataagga gaatatccgc
     4921 atgaaagtac cagccaactt gaaatatacg aaagatcatg aatgggctag aatcgagggc
     4981 gcggttgcgg tcgtcggtat taccgactac gctcaaagcc aattgggtga tgtagtttat
     5041 attgatttgc caaaagtcgg cgatgtggtg gcacgcggcg atgtaatcgg aacgatcgaa
     5101 gcggttaaga ctgttgctga tatttattcg ccggttagtg gtgaaatcgt tgccgttaat
     5161 tcagcgctaa aggatgcttc cgaactcgtc aataaagatc cttacggcga agggtggatt
     5221 gccagtatta aattatctaa ttcgatggaa ttggaagcat tactcgaccc ggcggattac
     5281 gagaaattaa tcagttagat tatgcacttc ataccgaata cctgcgatac ggaacgcgca
     5341 atcttggaga atctgggagt cgccagtttt aacgacttga ttgccaatat tccggaaaaa
     5401 ttcagggagc aatgccggat caatttaccg cccgctcttt cggaaattga gctaactaag
     5461 actatgcagg aactgtctga gaaaaatggt aacagtgata agcaagtgtc ctttctcggc
     5521 ggcggctctt atgatcactt catcccggcc gtggttgatt ttttaatttc gcggtcggaa
     5581 ttttatacag cctatactcc ttatcaagct gaagtcagcc agggcacact ccaggctatt
     5641 tatgaatacc agtcacaaat ttgtcatctg actgatatgg aggtcgctaa tgcctcgatg
     5701 tatgacggtg ccacggcttt ggcagaagcg gctattatgg cggcaaatat tacccaacgg
     5761 aaaaaatttc tggtttcgcc gctgattaat ccgcttgctc gtcaggttct ggaaacatat
     5821 ttgactgctc gtgatttcat ccttgccgac ttgccggaaa aaaatggcca aacagattta
     5881 tccgttctga aagaaatgat tgataatcag acggcggccg ttttagtgca atcgcccaat
     5941 ttttttggaa ttattgaaga tttaaactat attgccgaga ttattcatgc taaaagttct
     6001 ttattaataa tgtccgttga tccgatttca ttagccatcc taaaaacacc cggggaactc
     6061 ggtgcggata ttgccgtagc cgaaggtcag ccgctcggga ttcatcaatc gtttggtggt
     6121 ccttatctcg gtgttttcac cacgcgacgg caatatgttc gtaatatgcc cggccgaatc
     6181 gttggtcaga cgacagatgt cgaaggaaag cgcggttatg tattggtttt gcagacacgc
     6241 gagcaacata ttcggcggga aaaagccact tcgaatattt gtactaacca gggtttaatg
     6301 gcactagcgg ctaccatcta tttatctctt atgggtaaaa ccggattgcg gaaagtagct
     6361 gaactatgcc ttaataatag ccattatctg gcagataaaa ttgccggact gcccggttat
     6421 tctttgccct ttgccggtca gccgtttttt aaagagtttg tcgtaaaaac gccccgcccg
     6481 gctaccgaaa tcgcggcggc ggcgcttgca accggttttt ttgcggggat tgatctcggt
     6541 caatatcgtc gtgaatggga aaattatttg ttgatcgccg tcacagaaaa acgtaatttg
     6601 caagaaatta gtgattttgt aaaaattttg gctaaatttt aggtacaatg ggagtcttaa
     6661 atcaattact cgaaaaagcc cgcctgaatg gcggtggata ttttgtattg attgatcctg
     6721 acacacgctc gacgccggaa ttaaaagcgt tcgtgcgaga aatttgcgcc tgtggtgtgg
     6781 atgctatttt agtcggtggc agtttaatta tcggcggtgg attccacgaa accctggata
     6841 ttgtcaaaac tgaaagcgac gtgccagtga taattttccc cggttcggtc gctcaggttt
     6901 cgaaccgcgc cgatgcggtt ttatacattt ctctcatcag cgggcgcaat ccgaacgctc
     6961 tatttggcga acacgtaatc gccgcaccag ttatccgcaa attaggcatt gaaccgattt
     7021 ctaccggtta tatgctattc gaatccggca agactaccac ggctgaattt atgagtaata
     7081 cacgtcctct gccgatcaat aagcctgaaa tagcgatggc gcatgcttta gccgctcaat
     7141 atttgggaat gagtacagtt tatctggaag ctggtagcgg agccgagcgc tccatcccca
     7201 atcaactcgt gaaagccgtc tgtgattata tcgacatacc ggtattcgta ggcggtggta
     7261 ttcgtactcc ggacgaagcc cgtgccaaag tcgaagccgg ggcaacattc atcgtaaccg
     7321 gcaatgtctt tgataatgac gccaatatcg gattggctaa ggagtttgtc cgagctgtgc
     7381 atgtcaaaga gcatttttag actgaagcca tcttgcacca aaaaataatc gctcagttag
     7441 aagaaagcgc tgaagtaaag cgtcaaacaa tcataacggc ggttgactcg atcgaagccg
     7501 ccgcccaaat gttaattgat tgctatcgcg ccggcggcaa ggtgctttta tgtggtaatg
     7561 gcggcagtgc cgcggacgcg caacacttgg cggctgaatt aatttcgcga ttgaagttag
     7621 aacgagcggc gattccagcc ttagcgctga cgaccaatac ttctctattg accgctatcg
     7681 ggaatgatta caaatacgat ttggttttcg tacggcaagt ggaggctttt ggccgagctg
     7741 gagatgtgct aatcgccatc agtaccagcg gcgaatctga aaatgtgatc aaagccgtgg
     7801 aattttcccg cgtgcagggt atcaaatcca tagcgctgac cggtgcaaaa ggcgggcgtt
     7861 tagccgaaaa agttgatctt gcgattaaaa tcccttccga taacgttcag cgtattcagg
     7921 aatgtcatat taccgtcggt catattcttt gtgaattaat cgagtcagct ttatatggaa
     7981 ctatatgagc caattgatct taaatgataa gcgaaattca agattttatc aattatctca
     8041 aggtcgaacg taatctcgcc ccgaatacta tccaagccta cagcactgat ctggaacgct
     8101 atagtcaatt tctaacccaa caacaaatta atgacattag caaagtccgt tttagtgaca
     8161 tccaaaatta catcaatgta ttggctgaat tgggattagc ggccagaagt ctggcgcgta
     8221 attacagctc gattcgggct ttccatcgtt ttctatttag tgagcgcatc annnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntctttttct aagtatgcgc ggggctccgc
     8401 tcacccgaat ggcggtctgg aatattattc aggaatatgt acgtctggct gatattcgca
     8461 aaccgataag cccccacgtt ttccgtcatt catttgcaac tcatttattg gaaggaggcg
     8521 ctaatttgcg agcagtgcag gaaatgctgg ggcacgccga cattgcaacg acgcagattt
     8581 acacccatct cgaccgcgaa tttttaatcg aacaacaccg cacttttcac ccgaggtatt
     8641 aatgaacttt caagtaataa ttctgcttat tccgccgatt ctcctggctc ttacttttca
     8701 tgagtatatg catggctgga cggcttacaa aatgggcgat ccaacggcaa aaatggctgg
     8761 acgtctgaca atgaacccaa ttgcacatct cgatccgatc gggacattga tgctttttct
     8821 ggtacatttc ggctgggcac gtccggtgcc agttgatcca cgctattttc gtaatcccaa
     8881 gcgtgatatg ctgatagtcg ccgccgccgg accgttagct aatatgttta tagctttgct
     8941 gagcggttta ttggcgagac tttttcaggg cggatatttt agtttcctgc cgctaagtgt
     9001 gctacagccg ctttatgtga tggtaattct cagcttgcaa atcaacctgg ggctggcgat
     9061 tttcaaccta ctgccaattc ctccgctgga tggttccaaa atcctgatgg gactggttcc
     9121 accgcgttat gaagcccagt ttaactggtt agagcgatac ggcagtttta ttttaatggc
     9181 tgttataatt gtcgggatgg ttacgaattt ctctattctg ggagctttca ttaatccatt
     9241 cgtaaacttt ttctcgcaat tatttggagg tatctaataa tgtacacgag acccacaaaa
     9301 aaatttgaca tatctggcag acttgaggaa cagcgtgacg ggaaatttca ttttaaccgc
     9361 caaagtagag ttcctcggaa atcaccgcgc tctctgttat caactctgat atggatgatc
     9421 attatactat tggtgttgat gtttttaaaa aggctattat agagttcgtt agaaagcaca
     9481 gttatctttc atctcgttta gctaccagag taatgtatga ccgaaataat agaatgcgtt
     9541 cccaatttta gcgaaggtcg tgatcacgat gtaatcaggg caatcgcagt ggcaatcgaa
     9601 aaggtgaagg acgtaaaact actggatgtc aattctggcg ccagcgtcaa tcgcaccgtg
     9661 ataacctttg tgggcaatcg cgaaagcgtc attaatgggg cttttgctgg aattgccaga
     9721 gccgccgagc tgattgatat gcgtcatcat cgcggcactc atccgcgact cggagcgacc
     9781 gacgtctgcc cgcttgtccc tgtttccgga ataagcgtgg aagaatgcat tgctctttcg
     9841 caccaattag ctaaacgtgt cgctgaagaa ctacacattc cggtttatct ttacgcaaaa
     9901 tctgctcaaa aaccagaacg cattgatctg gcaaatatcc gcgccggaga atacgagggt
     9961 ctcaaacaaa aactcagcga tccgaactgg caacccgatt acggacaacc gattttcaat
    10021 cccaagtccg gggcgacgat tatcggcgtt cgtaatttcc tgatcgcctt caatgttaat
    10081 ctcgatactc gcgactgtca attagcaaag gacattgctc taacgattcg cgagaaaggt
    10141 cgaatcaaac gcgatcaaaa tggaaataaa atgtataatt cccaaggtga accaatttac
    10201 gaaaccggtt tattgaaaaa tgtcaaggcg gttggctggt acattgacga atataaaatg
    10261 gcgcaggttt cgactaactt gacgaattac aaagttactc cgccgcatgt cgttttcgag
    10321 gaagttcggc ggctcgccaa tgccaaaggt ctgcgtgtcg cgggttcgga actggtcgga
    10381 ctgattccaa aggaagcgct tctgatggct gggcgttatt atctgaaaaa acaaagccgt
    10441 tcggaagacg ttccagaaag cgaactcatc cagatggcag tgcaatcgct cggtttgaat
    10501 gccatcgcgc ctttcgatcc cgccaaaaag attatcgaat atcgtttggc ggagtnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaatga atggatttca gaattaatgt
    10681 cgaaaataaa aatattatcg gaaaatctga caaataaaat tgccgctgga gaagtcgtcc
    10741 agcgtccggc ttcgattgtc aaagaattag tcgaaaacag catagacgcc ggcgccgacg
    10801 aaattatcgt tgttattcgc aacggcggta aaacattatg cgaggtggtt gataacggtg
    10861 agggtatgaa tcgcgatgat cttctcctgg ctttcgaacg atatgctacc agcaaaattg
    10921 ccaccgtcga agacctgacc aatatccaca ccctcggttt ccgcggcgag gctctggcaa
    10981 gcattgcggc ggtagcaatc gttacggcaa tctcgactct gcggggcgca aatgaaggtt
    11041 atgaattact catcgaaggc ggcacgttcc gcgacatcag acccaccagt cccaaaccgg
    11101 gcacgacaat ttccgtccgt aatttgtttt acaatattcc cgcccgtcgc aaatttctga
    11161 gaagtgccga ggttgagttt cgtcatatcg tcgatgttat gcaagaattt gctctcatcc
    11221 accacgatct gcgttttatt tttatccaca atgatagaga aatttacaac ttgtcgccaa
    11281 gcagtctggc agaacgcatc gctaatctct ttgccccaga atatagtcaa aatcttctac
    11341 cggttgagtc ggtcgcagat gatctaaaag tcaccggcta tatcgggaac ctgaatctgg
    11401 tgcgcgcgcg gcgtggtgaa caatttttat acatcaatcg tcgctttgtg aatgatcgct
    11461 tgatgaatca tgcgataatc agcggctatg gcggattgct ggcgcggagc gaattcccat
    11521 tctattgcct gaacctagaa ctgccgccgg aacaaattga cgtcaatgtc cacccgacta
    11581 aaatggacgt aaaatttcgt gagcaagatc gcatttatca gtttttaaag caagccactg
    11641 cgaacagcct gaggtcaatt acaaacgtca ttcctgattt aggaactttt gcgccgcacc
    11701 attattatac tacacctccg ccgccgaaag gaacgcaaat tcagatcccg gctccggatc
    11761 aatctccttc taaacagtca gaatttgatt catcgagaaa aactttcgat gttccagaag
    11821 tagaaatccc gccgacaccg ttcgccgccg gcgacagcac caagtggcag gaacgggctc
    11881 ggcgttttgt cgagcagggc gagaccagcg aggctgatga ttttcgtccc agtgtagcgg
    11941 tttatcaagt ccataataag tatatcatca gtcaggtcaa gtccgggctg gtgattattg
    12001 atcagcatgt tgcccacgaa cggatcctct tcgaagaggc tcaggaatct atgcgcggtg
    12061 aaaattggaa agcacaacag ttgttgtttc cggtagttgt cgagctcacc gtgaatgatt
    12121 tttccactct tctggagata ttaccttttc tggaaaaact cggattccgt atgcgggaat
    12181 ttggcaaaca gacggttgcc gttgaagcgg ttccggccgg cttgcgctgg ggcaatgaaa
    12241 atatcatcat ccgcgaaata ctagatcact accaggaatt cggcaccaaa gacaccagca
    12301 ttcaggataa agtcgccgcc agttatgctt gtaaagccgc tatcaagtcc ggcgatccgt
    12361 tgactacgga ggaaatgcaa aacctggtgg atcgcttgtt tgcgaccagc aatccttatt
    12421 tttgtccaca cgggcgacca attatcgtca atctgtcgct gaaagaactg gataaacgct
    12481 tcgaaagggc ttagcaattg agtgctaatt ccaactcgat caatgaagcg ctttctcgaa
    12541 aaatcccttt tattgttggt cctacggcgg tagggaaaac tgcgactgcg attgaactgg
    12601 caaagcagtt gaatggtgaa attatctcag ttgattcgcg gcaagtatat agagggctag
    12661 atgttggcac ggctaagccg actttgcgac aacagcaaga aattccccac catttagttg
    12721 atattgtcga tctcgatgag cagattagcg ctggtgctta ccgcaattta gctttaaaaa
    12781 cggtcagtga aatccagtcc cgcgggagat tgcctatttt cgtcggcggt tccggattgt
    12841 atgttaatgc cgtgctgaaa ggaattttcc aagaaagcac tactaatcct gagatacgtc
    12901 gggaaatccg gcaagaatta caggaaaaag gcgttgtggc gctttataat cagctcctcg
    12961 aaattgaccc ggatacggtt ttgaaaattc atataaacga tgtcaagcgg ataacccgcg
    13021 cccttgaaat ttaccgcatc accggccagc cacccag
//
LOCUS       DAOOMU010000065        12855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_5365_length_12855_cov_15.464141, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000065 DAOOMU010000000
VERSION     DAOOMU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12855
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5365_length_12855_cov_15.464141"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLI55_07105"
     CDS             <1..198
                     /locus_tag="PLI55_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009012330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HPB00641.1"
                     /translation="TKINYLLFAIGIAVIIIGYICMATGETYSFRSLTVAPIILLIGY
                     LIIIPLAILYRKKEEKTPSES"
     gene            complement(249..539)
                     /locus_tag="PLI55_07110"
     CDS             complement(249..539)
                     /locus_tag="PLI55_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015274273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPB00642.1"
                     /translation="MPTYEYKCEKCGYEFEQFQKMSAEPLKVCPKCGQRSVTRKISGG
                     AGLIFKGSGFYVNDYGDKNKTKSPSPTTTEDKPTSTPKAKEKTITTSSNSSN"
     gene            complement(560..1537)
                     /gene="pta"
                     /locus_tag="PLI55_07115"
     CDS             complement(560..1537)
                     /gene="pta"
                     /locus_tag="PLI55_07115"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPB00643.1"
                     /translation="MKLIEEIRSQAQKCKKTVVLPEAHDERILKAAEILEKEGLAQII
                     LVGDPEIIGQHAREINVNLGSIGIFDPVQFEKLDDFINTYYEKRKHKGMTLENARTVM
                     LNPTFFGAMLVEKGLADGCVSGADTTTGDVLKAALHIIGTNPDTPTVSSDILIITPDE
                     ERIWSFADCAVLPNPTAEQLADIAIATANTHRRVVKTEPIVAMLSFSTKGSAKHELVD
                     KVIQATAIVRGKYPDLRIDGELQADAAIVPTIGAKKAPGSPVAGQANVLIFPDLNAGN
                     IGYKLVQRIGNCEAIGPIIQGLKKPMNDLSRGCSVEDVVNVTAIICNLV"
     gene            complement(1539..2018)
                     /gene="coaD"
                     /locus_tag="PLI55_07120"
     CDS             complement(1539..2018)
                     /gene="coaD"
                     /locus_tag="PLI55_07120"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPB00644.1"
                     /translation="MKIAVYPGSFDPITNGHLDILKRASSLFDLVYIAVARNLQKQPI
                     FTTEERLALIRGCTSEFENVKMDSFDGLVVEYARQVGAEVIIRGLRALSDFDYEFQMA
                     LMNRHLDENINTVFLMPHEDYTYLSSSMVRDLAKFGAEIGSFVPANVKAALLKKFRE"
     gene            complement(2019..2543)
                     /gene="rsmD"
                     /locus_tag="PLI55_07125"
     CDS             complement(2019..2543)
                     /gene="rsmD"
                     /locus_tag="PLI55_07125"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPB00645.1"
                     /translation="MVRVIAGLYKNQSIMSDKKATIRPTQDRVKAALFSILGDVSGLK
                     VADLFAGTGNLGIEALSRGAERCTFVDNNWQSLNLVKHNLAKLKLEQRAEVIQADVLK
                     YLAQKPLFDLIFADPPYEYSSYNELLMLFGQLRPDTQIVLETDRRFDLPSSFKPREIK
                     IRRSGDTNLNFIRI"
     gene            complement(2546..3730)
                     /locus_tag="PLI55_07130"
     CDS             complement(2546..3730)
                     /locus_tag="PLI55_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00646.1"
                     /translation="MIKINLAATPGLQQEPKDIAISQSLKDILIKMAVEPKNQPIDDQ
                     AISESSTKPIPEKVEKKTNVFASQGSNSSIPGGTDAEEPIPQPPPKISDKLREAEQII
                     ERKEEEYSKHLFNRPILNIGIWILVLVIVGALGYWGYTKYFNPRKSAITAKPAGKSDL
                     PVEQPILLTEQSQPSSTSDMPEVDQPVLTPINQIDEIYLPPVLTGISRCQILSQLISV
                     LPDNSRIQFLKLDKDVVNFLIFVDTENEAQQVKDEVQKLPGIGSIQAFYIEHVADTPS
                     PAVQMMAILNLKTESAVARNIRFYDDVSLSQVLWAIGKRQNLKLQPLNIAEPPAPKPR
                     LAEIRGNGSLNSIINLLSELSNFDLNLSVEKIAINLSADSAVDTGILDFSINSVIYPQ
                     KL"
     gene            complement(3739..4683)
                     /locus_tag="PLI55_07135"
     CDS             complement(3739..4683)
                     /locus_tag="PLI55_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00647.1"
                     /translation="MLAISLSDNFIRYGQLIKTDNSISIELIGKKKLPFKFDTTSLKN
                     PDLIPSIESLLLNIKNRLPVQERFLALSAPSQLFDITINSIDLGLEKQKIAEILDWNE
                     KQRLGVVFNKKFVQHYPLKQRISENKRDYLTISYFKELGRAIYQACQRLGFIIKVFDL
                     NIFSAANALESLYKEKNGKKWGLWLIGEKYHILLLIKSGEISQYLEFDLNNDGDYNIL
                     VDSNPDEDGKEIVSQINNLRNLSSETIQDIENLYFFTYDVNSEFYNMLLTYDIDNLRC
                     VDPFENFKPVKIYQDDGSGPGAMSQFLDVMGLLYRFML"
     gene            complement(4708..5490)
                     /locus_tag="PLI55_07140"
     CDS             complement(4708..5490)
                     /locus_tag="PLI55_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00648.1"
                     /translation="MSDETAKFNQEEEIIPEYKPTLVKVLNVVAFILIIVLIAVIYIP
                     SSIWKEENRIRELGRQRMQIQYDVQKYYNQMAGTHQPDPVLAMKVVSAVRDSTRADSN
                     FYGQKVIKLPDGKFNLEVPKNFYASFDTNFAFKYELSDTVIDTTIKITKWNPELMTID
                     TLYVISSMLEEVKTDPNFRSVIDTELTQRVANNTYYRRYYLTEDLAMRPLLDTPYTVL
                     KTEKGIRVEDPLKTVIREPRYLFFTFVDSSHGYIENGEPSWK"
     gene            complement(5483..5920)
                     /locus_tag="PLI55_07145"
     CDS             complement(5483..5920)
                     /locus_tag="PLI55_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPB00649.1"
                     /translation="MINLTRQEKMVIQLLVACFLVGATIRIYKMRSNRINNEKSRQQT
                     VVTDSFQLYASQIDSEYTELQKSGHSTYQSQKSTALQQKINLNTATKQELMLLPKIGP
                     VTADHILEYRAQVGKFNRIEELLNVKGIGTKTFEKIKGEVTLE"
     gene            complement(5977..7131)
                     /gene="dnaJ"
                     /locus_tag="PLI55_07150"
     CDS             complement(5977..7131)
                     /gene="dnaJ"
                     /locus_tag="PLI55_07150"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018126807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPB00650.1"
                     /translation="MAKDYYEILGVNRNAQADELKKAYRKIAMKYHPDRNPGNKEAEE
                     KFKEAAEAYAVLSDPEKRRVYDQYGEDGLKGTTGFSGAQSMNMEDIFSMFGDIFGGHF
                     GGFGGFDDLFNGGGRQRSTRTRTRSTQQRGSDLKITMPLTLEEINTGVDKKVKIKRQE
                     ICPNCNGTGAKPGSSNVTCPVCQGSGEVREVSRSFFGQMVNVRPCSNCQGEGVIVEQR
                     CPQCGGEGRIQSVKELTIKVPPGVTTGNYLTMHGEGNAGVRKSSRGDLIVIFEEKPHE
                     YFTREGDDIYLDLHLMPSEAVLGTEIVVPTLSGKVSLTIPPGTQPSKILRLKNKGLPH
                     LQHPGRGDMMVRIQVKIPERVSGKELELYRELFKLESKKIKDENRYSKIR"
     gene            complement(7133..7672)
                     /gene="grpE"
                     /locus_tag="PLI55_07155"
     CDS             complement(7133..7672)
                     /gene="grpE"
                     /locus_tag="PLI55_07155"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPB00651.1"
                     /translation="MNERTTGINKNQTRSTKKQTSEKERLSAKIAELEAKVAEYEKAL
                     NEANEKFLRLAAEFDNYRKRREKEYSDIIEYAGEEVLRNILPIMDDLERALNNINDDQ
                     SLREGLELIYRKFQKTLQNLGVEPIESVNKPFDPNLHHAIMTREEAGIAPDIVLEEYE
                     KGYKFKNRVLRYAKVVVSK"
     gene            complement(7988..8854)
                     /locus_tag="PLI55_07160"
     CDS             complement(7988..8854)
                     /locus_tag="PLI55_07160"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HPB00652.1"
                     /translation="MTILWFLIKEGFRGLGRSGYAGAFSIIIIALSLILIGFGYVAAR
                     DTLVMVENIRSQFDITIFVHRNASLTDLENFIEELSYFEEIEKIDYISTDSAAAQFKA
                     EFGEDIFSILDYNPLPPSFTLRLKPQARNLASIEDLTTRIKQYPIVDEVRYRENFLRL
                     LEKYQRLIFIAVVTAFIGLTLISIILASNSIKMTIFSRRNVIATMKLVGATNNFVRAP
                     FLIEGTLEGLIGALLASGVIGLISYFFNNYLQGLTGYQIIVSYRYYLGLLVLGSLIGL
                     IGSMRAIRRFLE"
     gene            complement(8851..9510)
                     /gene="ftsE"
                     /locus_tag="PLI55_07165"
     CDS             complement(8851..9510)
                     /gene="ftsE"
                     /locus_tag="PLI55_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HPB00653.1"
                     /translation="MITFFNVSCTFGKDAGVKDVNLTINEGEFVFLTGSSGAGKSTLL
                     RLIYMDLFPEKGKVIIRNFDSSKVRPNEIPFLRRKVGMIFQDFRLLEDRNVYENVALS
                     LYVSHYKTREIKNRVYNVLNQVGLGHRVYYNPRELSGGEQQRVAIARALVKEPVVLLA
                     DEPTGNLDPRVASDLMNLLIKINQTGTAVIVATHNYSLIPRVANARIIQVNEGEIINP
                     V"
     gene            9683..11056
                     /locus_tag="PLI55_07170"
     CDS             9683..11056
                     /locus_tag="PLI55_07170"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HPB00654.1"
                     /translation="MNKLRYWIITLILNVWCFSATGPCFDDYFLDQTMRLDFYFTGTK
                     TQNIFSLDAVYISPLWSGSQTNLIDTLNLGHHLIRVYDADSQTLIYSRGFSSIFNEWQ
                     STNEAIQEIWRTFHFSVLIPLPRQAITVTLSSRDRQNQFIEYFKTNIEPDSRFINRDG
                     VHTDYRLKKLIYNGSPTQKIDLLILPEGYTKSEMRQFRQKCRHFTEVLFNVSPFKEES
                     DKFNVWFLEVPSAESGIDNPRGGTFKRTAFDLTYNALDLDRYVLTLNNKTVRDIAAQA
                     PYDFIIFIFNSDKYGGGGIFNLYSTCYSTAAKPEQKGWPEYVFVHEFGHSFAGLADEY
                     YTSSTPYNELYPQGVEPWEPNITALLNPTALKWLDLVSPEVPVPTPWEKSAYEQATYS
                     KASEFEQFLKNQKYYGKVGAFQGAGYASEGLYRPCLDCIMFSRNEIAFCPVCQAAIRK
                     IIHFYSE"
     gene            11130..12107
                     /locus_tag="PLI55_07175"
     CDS             11130..12107
                     /locus_tag="PLI55_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB00655.1"
                     /translation="MPNLNSIEIKKRAIELYKLLDPCQICPRLCRAKRLHGAAGFCQV
                     LDKAILSSALAHFGEEPPITGRGGSGTIFLSSCNAACIFCQNYQISHERIGNPVSISQ
                     LANEYLHLQAVGCENINWVTPTPHLPFLVDALASAVEQGLKIPVVYNTNGYDRVEVLR
                     LLDGIVDIYLPDMKYGEDVWAEEYSGLKNYFTINVAAIKEMYRQVGLLIINEKGVAVK
                     GLLVRHLVLPNGRAGSARLFRALAKIDPQVPVSLMAQYHPCYQAVGHPVLGRRISRDE
                     YLKALEDFENAGLLNAFTQDYISLEHEDFFFPDFNDNERVFNHRNNREK"
     gene            12151..12459
                     /locus_tag="PLI55_07180"
     CDS             12151..12459
                     /locus_tag="PLI55_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00656.1"
                     /translation="MKKLLFIIIISAQVLTAQTLSLGLNGGWSQFKSSPTLTYYYDNF
                     STLMQTKSTTKLNSSGPTAGLVLQLNSKSERLALTGEILYSQLQGKTDSLWIIAPPYS
                     "
     gene            12505..12819
                     /locus_tag="PLI55_07185"
     CDS             12505..12819
                     /locus_tag="PLI55_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00657.1"
                     /translation="MATQIGLQWNVTRKSITPFLGTGLTANWFGILDVNVTSDSTLII
                     NNLDKHFDAGAFMSGGIRLKFKPVIVDAYVRYAMDNLFTGKIHKNGIEKFHLGLNLIY
                     SK"
BASE COUNT         3710 a         2711 c         2402 g         4032 t
ORIGIN      
        1 acaaagatta attatctact ctttgcaatt ggaattgcag taataataat cggttatatt
       61 tgtatggcta ccggtgaaac atatagtttc cgttcgctaa ccgtagcacc tatcatactt
      121 ttaatcggct atttgataat tattcccctg gcaattcttt atcgcaaaaa agaagaaaaa
      181 acgccctcag agagctgacc gaaattatta aaatcaagcg ctgatttcag ctgtttatga
      241 atataaattc agttggaact attagatgat gtcgttattg ttttttcttt agctttgggt
      301 gtggaagtag gtttgtcttc tgtagtggtt ggagatgggc ttttagtctt gtttttgtca
      361 ccataatcat tcacataaaa accggagcct ttgaatatta atccggcgcc accactaatc
      421 ttccttgtga cacttctttg tccacattta gggcagactt ttagtggttc ggcactcatt
      481 ttttgaaatt gttcaaattc ataaccacat ttttcacact tatactcata agtaggcata
      541 tatttttctg atcctatatt taaacgagat tacagataat tgctgtcaca ttaacgacat
      601 cttcaacgct acatcctcga gaaagatcat tcatcggttt ttttaatccc tgaatgatcg
      661 gaccgattgc ctcacaatta ccgatgcgtt gaactaactt atacccgata ttgccggcat
      721 tgagatccgg gaagattagt acatttgcct gaccggcaac aggactacca ggtgcttttt
      781 tagctccgat tgtgggtacg atagccgcat cggcttgtaa ctcaccatcg attctcaaat
      841 cgggatattt tcctcgcaca attgcggtag cctgaatgac cttatctact agctcgtgtt
      901 tggcactgcc tttagtagaa aaagaaagca tagcaacaat cggctcggtt tttacgactc
      961 tgcgatgagt attcgcggtc gcaatggcta tgtcagccag ctgctcggca gttggattgg
     1021 gcagcacagc acagtcggca aaactccata ttctttcttc atcaggtgtt ataatgagaa
     1081 tatcactgga aactgttgga gtatccggat tagttccgat gatatgcaat gcggctttaa
     1141 gaacatcgcc agtggttgta tcggctcccg aaacacagcc gtccgccaat cctttttcca
     1201 ccagcatagc tccgaaaaag gtcgggttta acattacggt gcgagcattt tcaagggtca
     1261 tccctttatg cttgcgtttt tcataataag tattaataaa atcgtccaat ttttcaaatt
     1321 gaacagggtc aaatatacct atcgagccga gattgacgtt aatttcacgg gcgtgttgac
     1381 cgattatctc tggatcacca actaaaatta tttgcgctaa gccttctttt tccaaaattt
     1441 cggcggcttt taaaatacgt tcatcatgtg cttccggtaa aacaaccgtt tttttacact
     1501 tttgggcttg agacctaatt tcctcgataa gtttcatttt actccctgaa ttttttcaac
     1561 agagccgcct tcacatttgc cggcacaaaa ctacctattt cggcaccaaa tttggctaaa
     1621 tctcgcacca tagaggaact tagataggta taatcttcat ggggcattag aaataccgtg
     1681 ttaatatttt catccagatg acgattcatt agcgccatct gaaattcata atcgaaatca
     1741 gataatgctc gtaagccgcg gatgattacc tctgccccga cttgacgcgc atattcgact
     1801 actaatccat caaagctatc cattttgaca ttctcgaatt cgctcgtaca gcctcgaatc
     1861 aatgccagcc tttcttccgt ggtaaaaatc ggttgcttct ggagattgcg agctacggca
     1921 atatatacta agtcaaataa agatgacgct cgtttgagta tatccagatg accattggta
     1981 ataggatcaa aactaccggg ataaactgct attttcattt atatccttat gaaattcaaa
     2041 ttagtatcgc cacttctgcg aatttttatt tcccgcggtt taaaagatga aggcaggtca
     2101 aatctacgat ccgtttcgag tacaatttga gtatcgggtc gtaattggcc gaacagcatt
     2161 aatagttcat tataactcga atattcatat ggtggatccg caaaaatcag gtcaaaaagc
     2221 ggcttttggg ctaaatattt caggacatca gcctgaataa cttcagcccg ctgttccaat
     2281 ttgagttttg ctaaattgtg tttaaccaaa tttaggcttt gccaattatt atcaacgaaa
     2341 gtgcatcgct cggcgccgcg ggatagcgcc tcaattccta aattaccggt tccagcaaac
     2401 aaatcggcga cttttagacc ggatacatcc cctaaaattg aaaataaagc cgcttttact
     2461 cgatcctgag ttggtcgaat tgtcgctttt ttatcggaca ttatggattg gtttttatac
     2521 aaaccggcaa ttacccgaac catctttaca atttttgtgg atagattaca gaattaatac
     2581 tgaaatcgag tattccggta tctactgccg aatcagcact taagtttatt gcaatttttt
     2641 caacacttaa attcaaatca aaattcgata actcacttaa taaattaata atactgttca
     2701 gtgagccatt cccacgaatt tccgccaaac ggggctttgg cgctggtggt tcggcaatat
     2761 taagcggctg caactttaaa ttttgacgct taccgattgc ccaaagcact tgcgagagtg
     2821 atacgtcatc ataaaaacgg atatttctag caaccgcact ctcagtcttt aaattgagaa
     2881 ttgccatcat ttggacagcg ggcgaaggag tgtccgcaac atgttcaata taaaatgctt
     2941 gaattgatcc aattcccggt aatttttgca cttcgtcttt aacctgctga gcttcattct
     3001 cagtatcaac aaaaattaag aaattgacca catctttatc taattttaaa aattgaattc
     3061 gtgaattgtc tggtaaaaca ctaattaact gggataatat ttggcaacga gatataccag
     3121 ttaaaacagg gggcagatat atttcatcaa tttgatttat cggggttaaa accggctgat
     3181 caacttctgg catatcggat gtagaagaag gctgggattg ttccgttaaa agaattggct
     3241 gttcaaccgg cagatccgac tttccggctg gtttagcggt aattgccgat tttcgtggat
     3301 taaagtactt agtatatccc caataaccta aagctccaac tataaccaga accaatatcc
     3361 agattccgat attaagaata gggcggttaa ataaatgctt ggaatattct tcttcttttc
     3421 tttcgatgat ttgttcggct tctctcagct tatcggaaat cttaggtggc ggttgaggaa
     3481 taggttcttc agcgtcggtt cctccgggta tacttgaatt tgaaccttgt gaagcaaaaa
     3541 cattcgtttt tttctcaact ttttcaggta tcggtttagt tgatgattcg ctaattgctt
     3601 gatcgtcaat cggttgattt ttcggctcga ccgccatttt gattagaata tctttaaggc
     3661 tctgactaat cgcaatatct ttaggttctt gttgtagtcc aggtgtagcg gcaagattaa
     3721 tttttatcat aattcgacct aaagcataaa ccgatataac aatcccatta catccaagaa
     3781 ctgactcata gctccgggac ccgatccatc atcttggtaa attttaaccg gcttgaaatt
     3841 ctcaaaagga tcaacacatc gtagattatc aatatcataa gtcaaaagca tattataaaa
     3901 ttcagaattg acatcataag tgaaaaaata aaggttttca atatcttgaa tcgtttcact
     3961 gcttaaattc ctaagattat ttatttgaga aacgatctct ttcccatctt cgtcgggatt
     4021 tgaatccacc aatatgttat aatccccatc gttatttaaa tcaaattcta aatattgact
     4081 gatttcaccc gatttgataa gtaggagaat atgatatttt tcacctatta gccataagcc
     4141 ccactttttt ccattttttt ctttatagag actttcaagc gcgttagccg cgctaaagat
     4201 attcagatca aagactttta tgataaatcc caaccgctga caggcttggt aaatagcccg
     4261 tcctaattcc ttaaaatatg aaattgttaa ataatcacgc ttattttcag aaattcgttg
     4321 tttcagagga taatgctgaa caaatttttt gttaaatact accccaagac gttgtttctc
     4381 gttccagtcc agaatctcgg caattttctg cttttccaat cccagatcaa ttgaattgat
     4441 cgtaatatca aataattgcg agggagctga taaagctaaa aaccgttcct gcactggtaa
     4501 cctattcttg atattaagca aaagggactc aatgctagga atcagatcag gattttttaa
     4561 ggaagtagta tcaaatttaa acggcagttt ttttttaccg atcaattcga tcgaaataga
     4621 attgtcagtt ttaatcagct ggccatatct aataaaatta tccgaaagac ttattgccag
     4681 catcttatct ccaaggtgat tggctatcta cttccaactt ggttcaccgt tctcaatata
     4741 cccgtgtgat gaatctacaa aagtaaaaaa gaggtagcgc ggttcacgga taacagtttt
     4801 gagagggtct tccaccctaa ttcctttttc ggttttaaga acagtgtaag gagtatccaa
     4861 tagagggcgc atagctaaat cctcagttag ataatatcta cggtaataag tattattagc
     4921 tacccgttgc gtgagttcgg tatcaattac acttctgaaa ttgggatcgg tttttacttc
     4981 ttcaagcatt gaggagatta catagagtgt atcaatggtc ataagttccg gattccactt
     5041 agtaatttta atagtagtat caatgaccgt atcggacaac tcatatttaa aagcaaaatt
     5101 agtatcgaaa gaggcgtaaa agttcttcgg tacttccaaa ttgaatttac catccggtag
     5161 tttgatcact ttttgaccat aaaaattgct gtcagcccgg gtgctatccc gaaccgcgct
     5221 gactaccttc atagctagaa ccgggtctgg ttgatgagtt ccagccatct ggttataata
     5281 tttctggaca tcatactgaa tctgcattct ttgacgacct aattcccgaa ttcggttctc
     5341 ttctttccag atcgaactcg gaatataaat tacggcaatc aaaacaatga tgagaatgaa
     5401 agccacaaca tttaatactt tcactagtgt cggtttgtat tccgggatta tctcttcttc
     5461 ctggttgaat tttgcagttt cgtcactcaa gggttacctc tcctttaatt ttttcaaacg
     5521 ttttagttcc gatacctttt acatttagga gttcttcaat cctgttgaat tttccaactt
     5581 gagcacgata ttccaaaata tggtcggcag ttaccggacc gatttttggc aataacatga
     5641 gctcctgttt ggttgcggta tttaaattta tcttttgttg aagagcagtt gatttttgtg
     5701 attgatatgt agaatgtcca cttttctgta actcggtata ttccgaatca atttgactgg
     5761 catatagttg aaaagaatca gtaactacag tctgttggcg gcttttttcg ttattaatcc
     5821 ggtttgacct cattttatag atacggatag ttgcacctac caggaaacaa gcaactaata
     5881 actgaataac cattttctcc tggcgggtta aatttatcat ttttcctaaa aattaaaaat
     5941 ctttaaaaat ctcattttct cttaatgtaa tattaattag cgaatcttac tataacgatt
     6001 ttcatctttt atttttttgg attccaattt gaataattcg cggtataatt ccaactcttt
     6061 gccactgact ctttcaggaa ttttaacctg aattctcacc atcatatcgc ctctgccggg
     6121 atgttgtaaa tgtggtaagc ctttattctt aagtcgcagt attttggacg gttgagtgcc
     6181 tggcggaata gtcaggctta ctttaccgct aagggttggg acgacaattt ccgtccctaa
     6241 aactgcctcc gatggcataa ggtgaagatc aaggtaaata tcatctcctt cacgagtgaa
     6301 atattcatgc ggtttttctt cgaaaattac aataaggtcg ccgcgcgaac ttttgcgaac
     6361 accagcatta ccctcaccgt gcatcgttaa ataattacca gtagtaactc ccggtggaac
     6421 tttaatcgtc agttctttaa cactctggat acgtccttcg cccccgcact gaggacaacg
     6481 ctgttcaaca attacgcctt cgccttggca attggaacaa ggacgcacat ttaccatttg
     6541 tccaaaaaac gaacgggaaa cctcccgaac ttcgccactt ccttgacaaa ccggacaggt
     6601 gacatttgaa gaaccgggtt tagcgccggt accgttgcaa ttggggcaga tttcttgacg
     6661 ttttatctta acttttttat caacgccggt attgatttct tctaaagtaa gtggcattgt
     6721 aatcttaaga tcactaccgc gttgctgagt cgaacgagtt cgagtacggg tagagcgttg
     6781 ccgcccaccg ccattgaata agtcatcaaa tccgccaaaa ccaccgaaat ggccaccgaa
     6841 aatatccccg aacatggaaa aaatatcttc catattcata ctctgtgccc cgctgaaacc
     6901 agtcgtacct tttagtccgt cttcaccgta ttgatcatat acccgtcgct tttctggatc
     6961 agatagaacg gcataagctt cagcggcttc tttaaatttt tcctcagctt ctttattccc
     7021 tggattgcga tcgggatgat atttcatggc aattttccgg taggctttct ttaattcatc
     7081 agcctgtgca ttccggttaa cacctaaaat ttcatagtaa tcttttgcca tattatttac
     7141 ttacaaccac tttggcgtat cttaaaacac ggtttttaaa tttatacccc ttctcatatt
     7201 cttccagaac aatatccggc gctattcccg cttcttcacg agtcataatc gcatggtgca
     7261 gattgggatc aaatggttta tttaccgact caatcggttc tacacctaaa ttctgcaagg
     7321 ttttttgaaa tttacgataa ataagttcca gaccttcacg caaagattga tcatcgttga
     7381 tgttatttaa agcccgctct aaatcatcca taatcggcag aatgtttcgc aaaacttctt
     7441 caccagcgta ctcgattata tccgaatatt ccttctcacg tcttttacga taattatcga
     7501 attccgccgc taaccgcaaa aatttttcgt tagcttcgtt caaggctttt tcatattcag
     7561 ctacttttgc ttccagttcc gcgattttgg cggataatct ttccttttca ctggtttgct
     7621 ttttagttga ccgagtctga tttttattta taccagttgt tctttcattc atacttatat
     7681 cttccacgat tcattctcct caaaattaat ttttaatcat ttaaataaaa ctcgttctga
     7741 attatgcaat tccaatacca gttataaaaa gttgaccgtt cgctcagtca aaaatgcgat
     7801 aattcggctt ggagaatcat tttttcggac tttctgacaa ttacagtttt tactcgggcg
     7861 cttattgtat ttttctatca tcataaaatc attcataact gcggaattta aattttctga
     7921 taggtcattc caaaataaat tgagtaacat tatagataat ttgaagggaa ttattctcag
     7981 gtatttctta ttcgaggaat ctacgaatgg cgcgcatact gccgattaaa ccgattaaac
     8041 tgcccaaaac cagcaatcct aaataatagc ggtagctgac gattatttga taaccagtaa
     8101 gtccttgtaa ataattattg aaaaaatagg agatcagacc gattacgcct gaagctaata
     8161 aagccccgat caatccctcg agcgttcctt caatcaaaaa aggcgctcgt acgaaattat
     8221 tcgttgctcc gactaatttc atcgtagcaa tgacatttcg gcgggaaaaa atcgtcattt
     8281 taatggaatt tgaagctaaa ataatcgaga tcaacgtcaa gccaataaaa gctgtgacta
     8341 cggcaatgaa tatcaagcgc tgatactttt ccagcaatcg caggaaattt tcacgatagc
     8401 ggacttcatc cacgatcgga tattgtttaa tacgagtagt aagatcctca atactagcta
     8461 gattacgggc ttgaggcttg agacgaagag taaatgaagg tggcagagga ttataatcaa
     8521 gaatactaaa tatatcttct ccaaattcgg ctttgaattg ggcagcggca ctatcagttg
     8581 aaatgtagtc aattttctca atttcttcaa agtaacttaa ttcctcaata aaattttcca
     8641 agtcggttaa tgacgcatta cggtgaacga aaatagttat atcgaactgt gaacgaatat
     8701 tttccaccat tactaaagta tcacgggcgg caacgtatcc gaatccaatt agaattagcg
     8761 atagagcgat aattatgatg gaaaaagcac cggcatagcc ggaacgccct aatccgcgaa
     8821 atccctcttt aatcaaaaac cacaatatgg tcatactgga ttaattattt ctccttcatt
     8881 tacctgaatt atgcgggcat ttgccacccg cgggatgagt gaataattat gcgtggcaac
     8941 gattacagcc gtcccagttt ggttaatttt aattaaaaga ttcatcaaat cggaagcgac
     9001 gcgcggatcg aggttgccgg tcggttcgtc ggccaataat actaccggtt cttttaccag
     9061 tgctcgggca atggcgaccc gctgttgctc gccaccggat aattcgcggg gattatagta
     9121 aacgcgatgt cccagcccaa cttgattcaa aacgttatag actcgatttt taatttcgcg
     9181 agttttataa tgggacacat acagactaag agccacattt tcataaacgt tccgatcctc
     9241 gagtagtcga aaatcctgga aaatcatccc gactttgcgt cttagaaatg ggatttcatt
     9301 cggtcggact ttagaactat caaaattgcg gatgataact ttgccctttt cgggaaacaa
     9361 gtccatatat atcaagcgta aaagcgttga tttcccagcg ccggaagaac cagttagaaa
     9421 aacaaactct ccttcattaa tcgtaagatt gacatcttta actccggcat ctttaccgaa
     9481 ggtgcaacta acgttgaaaa aagttatcat aatttaagtt ctaaaactcg gacaatttaa
     9541 acgcctgaat ataataaatc aacgccgttt cttctaaata ttatagttat acttcatagc
     9601 tactttaact caccgaaaaa ctttctcttg aaatctaaag tcagcttgaa taatttgcca
     9661 ataggaaaat ggagaaatgt caatgaataa attaagatat tggatcatta ccttgatttt
     9721 gaatgtttgg tgtttttcag cgaccggtcc ttgttttgat gactattttc ttgaccaaac
     9781 tatgcgactc gatttttact tcactggaac caagactcag aacattttca gtctcgatgc
     9841 ggtttatatt agtcctctct ggtcgggcag tcagaccaat ctgattgaca cgctgaacct
     9901 ggggcaccat cttattcgcg tttacgatgc ggatagccaa actttgatct actcccgcgg
     9961 ctttagcagt atttttaatg aatggcaatc aactaatgaa gctattcagg aaatttggcg
    10021 cacttttcat ttttctgtcc tgattccatt accgcgccaa gcgatcaccg taacgctttc
    10081 ttcccgtgat cgccaaaatc agttcatcga gtattttaag accaacattg agcccgattc
    10141 ccggtttatt aatcgggatg gcgtccatac tgattatcgg ttgaaaaagt taatctataa
    10201 cggatcgccc acccaaaaaa ttgacctgct aattttacca gaaggttata caaagtctga
    10261 aatgcgtcaa tttcgccaga aatgccgtca ttttaccgag gtcttattca acgtgagtcc
    10321 ctttaaagag gaatccgata aatttaacgt ctggttcttg gaagtgcctt cagctgaatc
    10381 gggcattgat aatccgcgag gcggtacctt taaaagaacc gcctttgacc tgacttataa
    10441 tgctcttgat ctcgatcgtt atgtattgac actcaataat aaaaccgtac gtgacattgc
    10501 cgctcaggct ccttacgact ttatcatttt tattttcaat tcggacaaat acggcggcgg
    10561 tggcattttt aatctttatt ctacctgcta cagtaccgcc gccaaaccag aacaaaaagg
    10621 ctggcctgag tatgtttttg tccacgaatt tggccattca ttcgccggtc tggcggacga
    10681 atattacacc tcgagtacgc cttataatga actctacccg cagggagtcg agccatggga
    10741 accgaacatt accgccctat tgaatccaac cgcattgaaa tggttagatt tggtttctcc
    10801 tgaagtccct gtgccgacac cctgggaaaa atccgcttat gaacaagcca cttatagcaa
    10861 ggcaagtgaa tttgaacaat ttctaaaaaa ccaaaaatat tatggaaaag tcggagcatt
    10921 tcagggcgct ggctacgcct ccgaaggttt atatcgtcct tgcctggact gcattatgtt
    10981 ttcacgcaat gaaattgcct tttgcccggt ctgccaagcg gcaatccgga aaataatcca
    11041 tttctatagc gagtaaagta atttcaatat aattaaatta ttgtaaagac atcaaaatgt
    11101 aaaataaatg atgaaataac acgctaacga tgccgaactt aaattctata gaaataaaaa
    11161 aacgggctat tgaactttat aaactgctcg atccctgtca aatttgcccg cgactctgcc
    11221 gggcaaagcg tttacacgga gcggcgggat tttgtcaggt gctagataag gctattcttt
    11281 ccagcgctct ggcacatttc ggcgaggaac cgccaataac cggtcgcggt ggttctggga
    11341 caatctttct gtcatcctgc aacgccgcct gtattttttg ccaaaattac cagattagcc
    11401 acgaacggat cggcaatcct gtatctattt ctcaattagc taatgaatat ttacatctcc
    11461 aggctgtcgg ttgtgaaaat atcaactggg ttacgccgac tcctcattta ccatttctgg
    11521 tggatgcact ggcttcggcg gttgaacagg gattaaaaat tcctgtcgtt tacaatacca
    11581 atggttacga ccgggtcgaa gtgttacgtc ttctggatgg tatcgttgat atatacttgc
    11641 cagatatgaa atacggcgag gacgtctggg cagaagaata ttcgggatta aaaaactact
    11701 ttaccataaa cgtagcggca atcaaagaga tgtatcggca ggttggacta ttgatcataa
    11761 atgaaaaagg tgtggcagtc aaaggtttgc tggtgcgcca tctggtttta ccgaatggtc
    11821 gcgccggtag cgctcgactt ttccgcgctt tagcaaaaat tgaccctcaa gtaccggtaa
    11881 gtctgatggc gcaatatcat ccttgttatc aagccgtcgg tcacccggtt cttggccgcc
    11941 gaatctcacg tgatgaatat ttaaaagcct tagaagattt cgaaaatgcc ggattattaa
    12001 atgcctttac tcaggactat atcagtctgg aacacgagga ttttttcttc ccggatttta
    12061 atgataacga acgggttttc aatcatcgaa ataatcgcga aaagtaaaat gccgtttcag
    12121 tagattatta aattttaata gagttgtaat gtgaaaaaat tattgtttat cattatcatc
    12181 agtgcacagg ttctcaccgc ccaaacgctg agccttgggt tgaatggcgg ctggtcgcag
    12241 ttcaaatcct cgccgactct aacctattac tacgataatt tttccacatt gatgcaaact
    12301 aagtcaacta caaaattgaa ttccagtggt cccacagccg gacttgtttt acagttaaat
    12361 tctaaaagtg agcgcttggc tttaaccggc gaaatattat atagccaact gcaaggcaaa
    12421 actgattcat tgtggattat tgcgccaccc tactcctgag cgatgtattc ttattcagcc
    12481 gggttggaga ccaggcagga cgttttggcg acacaaatcg gcttacaatg gaatgtaacg
    12541 cgtaaatcaa ttacgccatt tcttggtacg ggactaaccg ctaattggtt cggtattctc
    12601 gatgttaacg ttaccagcga ctctaccctt ataatcaata atctcgataa acatttcgat
    12661 gccggtgctt ttatgtccgg cggaattcgt ctgaaattca aaccagtcat cgtagatgct
    12721 tatgttcgtt atgcgatgga taatcttttc accggcaaaa tacacaagaa cggcatagaa
    12781 aaatttcatc tgggactaaa tctcatttat tcaaagtgag tctataaata ccgttgattt
    12841 accctaatat tcgat
//
LOCUS       DAOOMU010000066        12837 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_5376_length_12837_cov_13.303161, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000066 DAOOMU010000000
VERSION     DAOOMU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12837
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5376_length_12837_cov_13.303161"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PLI55_07190"
     CDS             <1..182
                     /locus_tag="PLI55_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPB00658.1"
                     /translation="RQVFQEQADYYQIELIIPSLKYCTDNAAMIARTGQEMLSRNQIS
                     PFSISAVPNLTLTTK"
     gene            184..>1936
                     /locus_tag="PLI55_07195"
     CDS             184..>1936
                     /locus_tag="PLI55_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00659.1"
                     /translation="MHSLTLKIPGFEWWWFILLLGLVGLLFFSFRKIPGGRSRSVTTI
                     LLVLRSLILILLAGLILKPEIHWTSSRKKAPSALVWIDNSLSIAAQNNFSKDSLLSIV
                     QEINTALDGKGIKPHLFLFDEKIIPVTGKLKNIENNGLATDLAQVLRYSKFEFENENI
                     VGALIISDGVITRGEDPTFSDIEMPFPVFTIGIGDSELVMDPAVTKIEMPQTVKAGDT
                     VTINAEIIPTGNGDPITVMLKEGEIILDKKVIASQSQAMRKMLSFQAIPSKPGEKIYS
                     VEITAAQDKNPYNNLRMNAVKVLESQIKILIVSGQANFEAPFLTRTLRGLQDVFVQNV
                     VINKGQWLPLSFQEIMAKEWNLIILIGYPTAESSQNDLIRLRQKISDYNLPLIFFLDG
                     DVDSKQLERLLGWNPIDELLADKSVNQISVKPTRAGLEHPLIKNFQKVNVSEIIWREL
                     PPIGSPYRRIRLKPSLLTIIESTDIAENPVIAVNQERNKPLALCIGLDFWRWSFMTNE
                     IGRFDIYDELFQGMTKYLTDTLSSSPIQLSINKRIFLNGESVEMSCLLYDLKGLVVND
                     AVVKAELIQQNDVIASSN"
     assembly_gap    1937..2036
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2037..2462
                     /locus_tag="PLI55_07200"
     CDS             <2037..2462
                     /locus_tag="PLI55_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00660.1"
                     /translation="AVVKAELIQQNDVIASSNLIWNGNNYSGVIPVRNAGDCQVRISA
                     WSGDNLIGIREQPITVVDRSIELLEVRQNVDLLRTIALRSGGLKTNRIPEIIDRLQVT
                     EKIITQSHVIKFLNWRWTLVILIVLLTVEWSMRRFQGFL"
     gene            2623..>3875
                     /locus_tag="PLI55_07205"
     CDS             2623..>3875
                     /locus_tag="PLI55_07205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013256803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPB00661.1"
                     /translation="MKKIAMLGVGYVGLVSGTGLADFGNEVICYDIDTTKIEKLNNGE
                     MPIYEPGLIDLVRKNVAKGRLKFTDDLTLAIRESEVIFLAVPTPSGENGEVDLSYIFN
                     AVDQIAKVIDSFKVIVTKSTVEVGTNDQIRERLLSHKIAPEMFEVVSNPEFLREGSAV
                     SDFMRPDRIVIGTSSPKALEIMKEIYRPLYLIETPIVATDIHTAEMIKYASNAFLATK
                     ISFINEIANLCELVGADVQQVAKAMGLDGRIGKKFLHAGPGYGGSCFPKDTLALTKIA
                     EKAGMRSTIVEAVIEANNRQKLRVVEKLLKVLPNLSDKTIGILGLAFKPNTNDVRETP
                     ALTVIEEILKRGGQIKAYDPEAMKEMRKYYPQITYVASTEDAAKGADALIIMTEWNEF
                     RNLDLNYLKSVMKNPIMIDSRNIYDP"
     assembly_gap    3876..3975
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3976..4900
                     /gene="rfbB"
                     /locus_tag="PLI55_07210"
     CDS             <3976..4900
                     /gene="rfbB"
                     /locus_tag="PLI55_07210"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPB00662.1"
                     /translation="VRVINLDKLTYAGNLANLQDLADNSDYIFVKGDICDADTVNNIF
                     RKYHPDYVVNFAAESHVDRSIGKPDDFIRTDMFGVFVLLEASKEFGIERFIQISTDEV
                     YGSILEGSFTEESPLKPGNPYSASKAGGDRLAYSYFNTYNLPVIITRASNNYGPYQYP
                     EKLIPLFVTNLIEDKPVPLYGDGKNVRDWLYVWDHCDAIEFILKNGKPGEVYNIAGEN
                     ELENIFITNFIFEYMNKPKTLIKPVADRLGHDRRYSISAAKLHELGWQAQHKFEDAMR
                     NTIQWYIDNPQWWKPLKSGEYLEYYKRHYKI"
     gene            4912..6150
                     /locus_tag="PLI55_07215"
     CDS             4912..6150
                     /locus_tag="PLI55_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPB00663.1"
                     /translation="MKKAFVILMVAILFDISLGQQYRSSESQKSYRSYGKMFEQETEK
                     EKTTLKEKAKEVSKMQELIALDKAVSPEEYIVGPGDIFGIDIIITENINIELAITPTG
                     DLLIPSVGKINVNGLILKDAIELCKKKINEVYPSAKVDVALVNLRTFKIQIAGAVFQP
                     GFYDVTAVTRLNEIIELAEGFDQFAKEFNIQITRNDGRIDTINYFNYKLEGDLKHNPT
                     FLEGDRIFVPFGDPDKEAIVIRGAVYGSGYDIIQENESLGYYLRRQAQFIENADLENV
                     TITRKEADHQLVMTVYPRDFFSTPLKPGDAIDILSERGISVNGFVQSPGSFYFYPGYS
                     YLDYINMAGGNTYEGDIDKVYIRHLDGSTEPGKSATLKRGDVIIIPRSSKSTIFGDLS
                     VLEIFTSIATILLTFIAAMK"
     gene            6189..7181
                     /locus_tag="PLI55_07220"
     CDS             6189..7181
                     /locus_tag="PLI55_07220"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HPB00664.1"
                     /translation="MAPIKKEHPLLYFIFKHRVTNLLILLLGAILVFVIVFFGMKKKY
                     TATVSILPPSQDLSSGIGSSMGALANLAGINLPQSTLQSPEMYKEILTSRKVLDTILF
                     EEFEGVDSSGTIYRKKLIDMLDIKAKNEREKIDKALKKLLKDVIYVNINNESNVTKLS
                     VTLTDPFYAANVANRMVELLNEIVVTQIQHENRQQLLYIEDHIKNTQDSIKIAEKYLQ
                     TFLEQVKSIAEPANQIKELALKRDLELYSTIWIELKQQKEIYTLKSIPSLAEVKVLDI
                     AQAPYLKSRPKRLLTMISLGGLFLILQICVNALILIINNFRKELKPDEPIPHPK"
     gene            7156..8463
                     /locus_tag="PLI55_07225"
     CDS             7156..8463
                     /locus_tag="PLI55_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPB00665.1"
                     /translation="MNQFPTLNELKGFFSNKEGKSLFANFAQFGIYQVIIYLAPLITV
                     PYLLRYIGVAKFGIISFAMALVYYFQIVVEYGFGITGIQMIAQSQNNSRKQSEIVSNV
                     LIIQIILFSICFILLGILCFLIPKIHDYRSIYLFSYGYVLANIFLFTWFYIGVEKVKY
                     VNVIISISKLLYILLIIIFIKNENDFVLVPIFNSATTFLAGIVSVIILRKKFIIRFLK
                     PNKDILKRYLKEGWHIFLSNIAMNLYRNTNVIILGIFSSDISVGIYSAGEKIVKAIQS
                     TLSPLTQTLYPFISRKKIEEPEKSIRYISRMITIMGFIGCIISILLFIFAGKIAYLLN
                     GSVNLAMVRVIRIASFVILFGGLNYCIGIIFMTNYDMKKEFLKSVMIVGILNTIICAI
                     LCHLISEIGAATALLLSEFILFMLLVYHIQHNKYKWSTNPLEL"
     gene            8460..9524
                     /locus_tag="PLI55_07230"
     CDS             8460..9524
                     /locus_tag="PLI55_07230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB00666.1"
                     /translation="MSYPHVSIIILNWNKWADTIECLESVLKSDYPNFNIILVDNGSD
                     NDSVEKILAWADGKLIKPYISNYDEIVLPEVKKPLIIQKIQIENINHVPTESGKNAMI
                     NLLISNENLGFAKANNLSIRYADATWNSKYYYLLNNDTVIEKETLRNLVTVLENETLY
                     KIAQSTIFYYNNKEEIACAGGRLFPWGKGKFYTRITSDEIKEIGFINGCALLISQEIV
                     EKYGLLSERFFFGEEDFEYSYRLSLAKVKKVCVGNSRVYHKISVSSNHLLDNDRGRSL
                     VLSALNRIADMRNYYPRFIWIFWRYLTVIYYYLFLMIIKHGIDTKLANKYLKYILKKS
                     RNISSVDKTLYEEIINKKWN"
     gene            9515..10789
                     /locus_tag="PLI55_07235"
     CDS             9515..10789
                     /locus_tag="PLI55_07235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6337 family protein"
                     /protein_id="HPB00667.1"
                     /translation="MELTKLSSLFFAAVILAMTIFEKRRLNTIITPFSVSAWPLVFII
                     LVVNFMLVKIEFDIVTPRAILFLLANLFIIWLIGIIFSYIHNNDRIQSLNDIFLPYVK
                     FEKILIVLSWVVNIVVFLKMKNLLAKYGGLSFLGDYRFEEMINSGPEAHISQFGKVTF
                     LLLSFIYPMANHKKTILITLFGSIVMLFMLQSKYNLIGVLVIVFIYYNFTKPVKKQIG
                     SILKFSLLTLMIMALSLITLTIFWGTFKVSKQSVWLFLSKNLLSYFFSSPIVLDKWLD
                     HAFVQPDWTLLLLFKNIINRIVGNPLVYSSLDYVDLNFSEVAKDVYSNVGTSYGTYFI
                     IGGWIFSLFMSTVISSLTYFVYYSAKKKPNPVNVFLCMTLLNIALFSFFGQYFTFLPY
                     YEMIIFYIVTIKVMDTYNLFISDYQNSLPLRK"
     gene            10789..11898
                     /locus_tag="PLI55_07240"
     CDS             10789..11898
                     /locus_tag="PLI55_07240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB00668.1"
                     /translation="MFRKIKILHVVNVFGGAVGRILVDVMPHLVKKFDVKVVSIQSID
                     YSEPLVQEYIRKYNNLESLKLNRFSLFKAYHKLNCYFKKIDPDLIHSHMGRADIFTAM
                     SKTKNMIIMNTLHCEKKNHNPLTQIGYRLTDRRVDWRICISQTVARSYYNHGFKSPYS
                     VIYNPIDINGFTKPINREEIRNNLGFKPNEQIIINVGRLIKCKGQRYLIEVMPHLCHL
                     LPEIKLLIVGDGPQYNSLKKLIINNNLENNVYLLGYRQDVQNLLKISDLFVYSAQWEG
                     LGMAVIEAMATRIPVLAHPLPALKEYIIDNHNGYFCNIHLKESFANKIYDILTNSHHN
                     SSIAENAFRTVTDMFSADKISKQYIELYRQLLSNR"
     gene            11941..>12837
                     /locus_tag="PLI55_07245"
     CDS             11941..>12837
                     /locus_tag="PLI55_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB00669.1"
                     /translation="MLVSNTSWYLYNFRLPLLKSLKEKGFKVITIAPKDEYTSRLISN
                     GFTHYHIPIDRRGLALFPNLITILSFISLYYRLKPKMIIHFTIKPVIFGTITARFCTK
                     ARIVNNVTGLGYIFIGETFLHTLLQPFVQWMYRCVLRLSDLLIFQNIDDRIFFIKSKI
                     ITEKIPPSILISGSGVDLERFQRKNNKNKKSEVNFILVARMLYDKGIREYIEAAKIVK
                     ISFKNAVFHLLGDIDKGNPSAIPIEIINTWSKQGIVNYYGMVEDVVPFLENADVFVLP
                     SYREGLSRSILKLWLWNSQLLPP"
BASE COUNT         4195 a         2078 c         2379 g         3985 t
ORIGIN      
        1 taagacaagt tttccaggaa caggctgatt actaccaaat cgaattgata attccatctt
       61 tgaaatattg tacggacaat gccgcgatga tcgccagaac cgggcaggaa atgttgtcac
      121 gcaaccaaat ttcgcctttt tcaatctcag ccgtaccgaa tctaacactt actactaaat
      181 aaaatgcatt cattgacctt gaaaattccg ggatttgaat ggtggtggtt tatcctttta
      241 ttgggattag ttggactttt attcttctcc ttcaggaaaa ttcccggcgg aaggtcgcgg
      301 tcagtaacga cgatattgct ggttttacgc agtttaattt tgattctact ggcaggattg
      361 attcttaagc cggaaattca ttggacaagt agtcgcaaaa aagcgccatc agctttggtt
      421 tggattgaca attcgcttag cattgccgct caaaataatt tttctaaaga cagtttgctt
      481 tcaattgtcc aggaaattaa tacagcactg gatgggaaag gaatcaagcc gcatcttttt
      541 ctatttgatg aaaaaattat tcccgtaacg ggaaaactaa aaaatatcga gaataatggc
      601 ctcgccaccg acttagccca agttttacga tattctaagt tcgagtttga gaacgaaaat
      661 atcgtaggtg cgctaattat ttccgacggt gtcatcaccc gaggcgaaga cccaactttt
      721 agcgatatcg aaatgccgtt tcccgtgttt actattggaa ttggcgatag cgaactggtt
      781 atggatcctg ccgttacgaa aattgaaatg ccccaaaccg tcaaagctgg agatacagtt
      841 actatcaatg ctgaaataat accgactgga aacggcgatc cgattactgt aatgcttaaa
      901 gaaggtgaaa taattcttga taaaaaagtc atagcgagtc aaagccaagc tatgagaaaa
      961 atgctgtctt ttcaagcaat tccgtccaaa ccgggcgaaa aaatatattc tgttgaaatt
     1021 acagccgcgc aggataaaaa tccctacaat aatctcagga tgaatgcggt gaaagtgctc
     1081 gaatcccaaa ttaaaatact cattgtcagc gggcaggcta atttcgaagc gccttttctg
     1141 actcgcactc tacgcggatt gcaagatgtt ttcgttcaga atgtagttat aaataaagga
     1201 cagtggttgc cgctctcatt tcaagagata atggcaaaag agtggaattt gataattctt
     1261 atcggctatc cgacagccga atccagccag aacgatttaa ttcgtctccg gcaaaaaatt
     1321 tccgattaca atttgccact aatatttttt ctcgatggag atgtcgattc taaacagtta
     1381 gaaagactgc tgggctggaa tccgattgat gaactgttag ctgataaatc agtaaatcag
     1441 attagcgtca aaccaacgcg cgcaggtctg gaacatcctc taataaaaaa ttttcaaaaa
     1501 gtaaatgttt ccgaaataat ctggcgggaa ttaccaccga tcggctcgcc ttaccgacgg
     1561 attcgcctta agccgtcttt gctaacgatt attgaaagca ctgatattgc cgaaaatccg
     1621 gttatcgccg tcaatcagga aagaaataaa cctttggcgc tctgtatcgg attagatttt
     1681 tggcgctggt catttatgac caatgagata ggacggtttg atatttatga cgaactgttt
     1741 caaggaatga caaaatattt aaccgatacc ctcagctctt ctccaattca actgtcaatc
     1801 aataaaagaa tatttctgaa cggtgaaagc gtcgaaatga gttgcctgct gtacgatttg
     1861 aagggcttag tagtaaacga tgccgtagtc aaagccgaac taattcaaca aaacgacgtg
     1921 atcgcctcaa gcaattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngccg
     2041 tagtcaaagc cgaactaatt caacaaaacg acgtgatcgc ctcaagcaat ttgatctgga
     2101 atggaaataa ttattcggga gttatacccg ttagaaatgc cggcgattgt caggtacgca
     2161 tttcagcttg gagtggcgat aatttaatcg gaattcgcga acagccgatc acggtagttg
     2221 atcgctcgat cgaattactt gaggttcggc aaaacgtgga tttactcaga acaattgcct
     2281 tacggagtgg cggtttaaaa acgaaccgga ttcccgaaat tattgatcgc ttacaagtaa
     2341 cggaaaagat tatcacccag tcacacgtta tcaagttttt aaactggcga tggacgcttg
     2401 tgattttgat tgttttactt acagtagaat ggagtatgcg acgttttcag ggctttctat
     2461 aaaaagtctt gaccttttaa ttgtttatgt tcaaatttcc accgtgcaga ctagaattca
     2521 aaaaaagagg tatttcagca agaattggta aatccgatct ggtcttagca cgtaaaacgc
     2581 ttaccccatt tattaaatag aaaaaaatac cggagtgatt ttatgaagaa aattgcaatg
     2641 ctgggagtag gatatgtcgg tttagtatcc ggaacgggac tggctgattt cggtaatgaa
     2701 gtaatctgtt atgatattga tacgactaaa atagaaaaac taaataacgg agaaatgcca
     2761 atttatgaac cgggtttgat tgatttggtt agaaagaacg tcgctaaagg ccgactgaag
     2821 ttcaccgacg atctgacgct ggcaatacgg gaatcggaag tgatttttct ggcggtaccg
     2881 actccttccg gcgaaaatgg tgaagttgat ctttcctaca tttttaatgc ggttgaccaa
     2941 atcgccaagg ttattgacag ttttaaagtt attgtcacta aaagtacagt cgaggttgga
     3001 actaatgatc aaatccgcga acgtctatta tcacataaaa ttgcgccgga aatgttcgaa
     3061 gtggtttcaa atcccgaatt tttacgcgag ggttcggcgg ttagcgattt tatgcggccg
     3121 gatcggattg tgatcggcac cagttcacca aaagcccttg agattatgaa ggaaatttat
     3181 cgtcctctct atttaatcga aacgccgatt gtggcaaccg atattcacac cgccgaaatg
     3241 attaagtatg cttcaaatgc ttttttagcg acgaaaattt catttattaa cgaaatagct
     3301 aatctctgcg aactagtagg cgccgatgtt cagcaagtcg ccaaggctat gggtctggac
     3361 gggcggatcg gtaagaaatt tctacatgct ggaccgggtt acggcggctc ttgcttccca
     3421 aaagatactt tagctttaac taaaatagct gaaaaagccg gaatgcgatc cacaattgtg
     3481 gaagctgtca ttgaggccaa taatcgtcag aaattgcggg tcgtcgagaa gctcttgaaa
     3541 gttctaccaa acctctccga taaaacgatt ggaatcctgg gattagcctt caagcccaac
     3601 actaacgatg tccgcgaaac gccagcatta accgtaattg aagaaatttt gaaacgcggc
     3661 gggcaaatca aagcctatga tccggaagct atgaaagaaa tgcgcaaata ttatccccaa
     3721 attacttatg tggcttccac tgaagatgcc gctaaaggag ccgatgcttt gattattatg
     3781 acggaatgga acgaatttcg caatttggac ttaaattatc taaaatcagt gatgaaaaat
     3841 ccgattatga ttgatagtcg caatatttac gatccnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnntgttc gggtgattaa tctcgataaa cttacatatg ccggcaacct
     4021 ggcaaatctg caagatctgg ctgacaattc cgattatatt tttgttaaag gcgatatttg
     4081 tgatgcggat actgtaaata atatttttcg taaatatcat ccggattacg tcgtgaattt
     4141 tgcggccgag tcgcatgtag atcgtagtat cggtaagccg gacgatttta ttcggacgga
     4201 tatgtttggc gtttttgttt tattggaagc ttccaaagaa tttgggattg agcgtttcat
     4261 ccagattagt acagatgaag tctatggctc aattttggaa ggttcattta ccgaagaatc
     4321 gccattgaaa ccgggtaatc cttattcagc cagcaaagcc ggtggcgatc gtctggcgta
     4381 ttcctatttc aatacttata acttgccggt aattattacg cgcgcttcga ataattacgg
     4441 tccttaccaa tacccggaaa aactcattcc gctgtttgtc acaaatttaa tcgaggataa
     4501 acccgttccc ttgtatggtg atggaaaaaa tgtgcgtgat tggttgtacg tctgggatca
     4561 ttgtgatgcg attgaattca ttctgaaaaa tggaaaaccc ggcgaagttt ataatatcgc
     4621 cggagaaaac gagttggaaa acatcttcat caccaacttt atttttgaat atatgaataa
     4681 accgaagaca ttgataaaac cagtcgcgga ccgactggga catgaccggc gttactcgat
     4741 ttcggcggct aaactgcacg aactcggctg gcaagcacaa cacaaattcg aagatgctat
     4801 gcgcaatact atccagtggt atattgacaa tccgcaatgg tggaaaccat taaaaagcgg
     4861 cgaataccta gaatattata aaaggcatta taaaatctaa gaggtatatt aatgaaaaaa
     4921 gcatttgtta ttctaatggt ggctatttta ttcgatattt cattagggca acaatataga
     4981 tcctccgaat ctcaaaaatc gtatagatcc tatgggaaaa tgtttgaaca agagactgaa
     5041 aaagaaaaaa ctactttaaa agaaaaagca aaagaagttt caaaaatgca agaactaatt
     5101 gctcttgata aagcagtttc acccgaagaa tatatcgtag gccctggtga tatatttggg
     5161 attgatataa ttatcactga aaatataaac atagaattag ctataactcc tactggggat
     5221 ttattaatac catcggttgg aaaaattaat gtaaatggtt taattctgaa agatgccatc
     5281 gaactatgta aaaagaaaat caatgaagtc tacccaagtg ccaaagtcga tgtagcattg
     5341 gtaaacttac gcactttcaa gattcaaatt gccggcgccg tgtttcaacc agggttctat
     5401 gatgttactg cggttacaag attaaatgaa ataattgagc tggctgaggg atttgaccaa
     5461 tttgccaagg aatttaatat ccagataact agaaacgatg gcagaattga cacaataaat
     5521 tactttaatt ataaactcga gggagacctg aagcataatc ctactttttt agagggcgat
     5581 agaatattcg tcccatttgg agatccggat aaagaagcta tcgttattcg aggtgcagtg
     5641 tatggaagtg gctatgatat tatccaagaa aatgaatcct taggctatta tctacgacgc
     5701 caagctcagt ttattgaaaa cgctgactta gaaaatgtaa caattacgcg aaaagaggct
     5761 gatcaccagc tagtaatgac ggtatatccg cgagatttct tctccactcc attaaagcca
     5821 ggtgatgcaa ttgacattct ctcagaaaga ggaatatcag ttaatggatt cgtccaatct
     5881 ccaggtagtt tttattttta tccgggctat tcatatttgg attatattaa tatggctggt
     5941 ggaaacacct atgaaggaga tatcgataag gtttacatac gtcacttgga tggaagtacc
     6001 gagccaggta aatctgcaac tcttaaacga ggtgatgtta taattatccc tcgtagttct
     6061 aagagtacaa tattcggaga tttatcagtt ctggaaatat ttacttccat tgcaactatt
     6121 ttattaactt ttattgccgc aatgaagtaa atgaaattta aaattttcat aaataacagg
     6181 agaattacgt ggcacctata aaaaaagaac atccgcttct ctattttatt tttaaacatc
     6241 gcgtcacaaa cctattaatt ttactccttg gagctatcct agtttttgta atcgtatttt
     6301 ttggaatgaa gaaaaaatat accgctacag tgtcaatttt acctcccagc caggatttat
     6361 catcaggtat aggcagctcg atgggtgctt tggcaaattt agctgggata aatttacctc
     6421 agagtacact ccagagtcct gagatgtata aagaaatcct tacctcaaga aaagttttag
     6481 atacaatcct atttgaagaa tttgaaggag ttgattccag cggtacaatt tatcgaaaaa
     6541 agttaattga tatgcttgac atcaaagcca aaaatgaaag agaaaaaatt gacaaggcat
     6601 tgaaaaaatt actcaaagat gttatttatg taaatatcaa taatgaaagt aatgttacta
     6661 agttatcggt gacacttacc gatccttttt atgcggctaa tgtagccaat aggatggtag
     6721 aactattaaa tgaaattgtc gtaacccaaa tccagcatga aaatcgtcaa caattactat
     6781 acattgaaga tcacatcaaa aatacccaag acagtattaa aattgcagaa aaatatttac
     6841 aaacattttt agagcaggta aaatcaattg ctgagccagc taatcagatc aaagaattgg
     6901 cattaaaaag ggatctggaa ttgtattcta ctatctggat cgaacttaaa cagcagaaag
     6961 aaatttatac attaaagtca atcccaagcc tggcagaagt taaagtcctt gatatagccc
     7021 aagcacctta tttaaagagt aggcctaaaa gattactaac tatgatcagt ctgggagggc
     7081 tctttctaat tttacaaata tgtgtaaatg ctctcatttt aattattaat aattttcgca
     7141 aagaactcaa gcctgatgaa ccaattcccc accctaaatg agttaaaggg atttttctct
     7201 aataaagaag gtaaaagttt atttgctaat tttgctcaat ttggcatata tcaggttatt
     7261 atttatttag cacctttaat tactgttccg tatctcttga ggtatattgg agtagcaaaa
     7321 tttggtatca tcagctttgc aatggctctc gtctattatt ttcaaatagt cgtagagtat
     7381 ggatttggga taacgggaat ccagatgatt gcccagagtc agaataatag taggaaacaa
     7441 tcagaaatag tatcgaatgt tttaataatt caaattatac tattcagtat ttgttttata
     7501 ctattaggga tattatgttt tttgattcca aaaatacacg attatagatc catttatcta
     7561 ttttcctacg gctatgtctt agcgaatata tttttattta cctggtttta cattggagtg
     7621 gaaaaagtca aatatgtcaa cgtaattatt tctatatcaa aattattata tatactctta
     7681 attatcattt ttattaaaaa tgaaaacgat tttgttttag tgccgatatt taattcggcg
     7741 actacctttc tggcggggat tgttagcgtt atcattttaa ggaaaaaatt catcatccgt
     7801 tttctcaaac cgaataagga tattttgaag agatatttaa aagaaggttg gcacattttt
     7861 ctctcaaata ttgctatgaa tttatatcgc aatactaatg ttattattct gggaatcttt
     7921 tcatcagata taagcgtggg aatatacagt gccggtgaaa agatagtcaa agcaatccaa
     7981 agtaccttat cacctcttac tcaaaccttg tatcctttta tctcccgaaa gaaaattgaa
     8041 gaaccggaaa aatccataag atatatcagt agaatgatta cgattatggg ttttattgga
     8101 tgtattatct caatattact atttatcttt gcagggaaaa ttgcttatct tttaaatggg
     8161 tcagttaatc tggcaatggt acgagtgatt agaatcgcct cttttgtaat tttattcggg
     8221 ggtttgaatt attgtattgg aataattttt atgacaaact acgatatgaa gaaagaattt
     8281 ttaaagagcg tgatgattgt gggaatattg aatactataa tttgcgcaat tctttgtcat
     8341 ttaatcagcg aaattggcgc ggcaactgct ctattgcttt ccgaatttat tctatttatg
     8401 cttttggttt accatattca acataataaa tataaatggt caacaaatcc cttagaatta
     8461 tgagttatcc acacgtatct atcataattt tgaactggaa taaatgggct gatacgattg
     8521 agtgcctgga gtcggtattg aaatcggatt atccgaattt caatattatt ctggttgata
     8581 atggctcgga taacgattca gttgaaaaaa tattagcatg ggctgatgga aaattaataa
     8641 aaccttatat tagtaattat gatgaaattg ttttgccgga agtaaagaag cctttaatta
     8701 ttcagaaaat acagattgag aatattaatc acgttccgac tgaatctggt aaaaacgcta
     8761 tgattaatct gttaataagc aatgagaacc ttggatttgc taaagctaat aacttaagca
     8821 ttagatatgc tgacgcaaca tggaatagta aatattatta tttattaaat aatgatacgg
     8881 tcatagagaa ggaaacccta agaaatcttg taactgtatt agaaaacgaa actctatata
     8941 aaatagctca atcgaccata ttttattata acaataaaga agaaattgct tgtgcgggcg
     9001 gtaggttgtt tccttgggga aaaggtaaat tttatacccg gattacgtcg gacgagatca
     9061 aagaaatagg atttataaac ggatgcgctc tattaatttc gcaagaaatt gttgaaaaat
     9121 atggtttact tagcgaaagg tttttctttg gtgaagaaga ttttgagtat agctacagat
     9181 tgtctttagc aaaagtaaaa aaagtatgtg tcggaaatag tcgagtgtat cataaaatta
     9241 gcgtctcttc taatcactta ctagataatg atcgcggtcg tagcttagtt ttatcagcat
     9301 taaacagaat cgcagatatg cgaaattatt atcccagatt catctggata ttttggcgct
     9361 atcttactgt catttattat tatttattcc tcatgattat taaacatgga atcgatacta
     9421 aattagccaa taagtatttg aaatacattc tgaaaaaaag tagaaatatt agtagtgttg
     9481 ataaaacatt atacgaagag ataattaata aaaaatggaa ctgactaaat tatctagtct
     9541 cttttttgcg gctgttatat tagctatgac gatttttgag aagcgtcgct tgaacacgat
     9601 aattacaccc ttttccgtat cagcgtggcc tttagtgttt atcatactcg tagtaaattt
     9661 tatgttagtg aaaatcgaat ttgatattgt aacaccacga gcaatattat tcttattggc
     9721 gaatttattt attatatggc taattggcat aatattttca tatattcata ataacgatag
     9781 gatacagtca ctaaatgata tttttttacc gtatgttaaa tttgaaaaaa tccttatagt
     9841 tctttcctgg gtagtaaata ttgttgtatt tttaaagatg aagaaccttt tagctaaata
     9901 tggcggttta agttttttag gagattacag atttgaagaa atgataaaca gtggacctga
     9961 agcacatata tcacaatttg gaaaagtaac gtttctacta ctgagtttta tttatccaat
    10021 ggcaaatcat aaaaaaacta tccttattac actttttgga tcaattgtta tgttatttat
    10081 gcttcagtca aaatataatc ttattggagt tttagtaata gtatttatct attataattt
    10141 tacaaaacca gttaaaaagc agattggcag tattttgaaa ttcagtttat taacgttaat
    10201 gataatggct ctttccttaa tcacattgac catattctgg ggtactttta aagtatcaaa
    10261 acaatcagta tggctattct tatcaaaaaa cttactcagt tattttttct catctccgat
    10321 tgttttggac aaatggttag accatgcttt tgtgcaacct gattggacat tactattact
    10381 atttaaaaat ataataaacc gtatagtggg caatccatta gtatatagct cgttggatta
    10441 cgtagactta aacttttcag aagttgccaa ggatgtttat tcaaacgttg gaacaagcta
    10501 tggaacgtat tttatcattg gaggttggat ttttagttta tttatgagta cagtaatctc
    10561 ttcattaacc tattttgttt attacagcgc caagaaaaaa cctaatcctg tgaatgtatt
    10621 tttatgcatg actttattaa acattgcgct ttttagtttt tttgggcaat attttacatt
    10681 tctaccatat tatgaaatga ttatatttta tatagttacc ataaaggtaa tggataccta
    10741 taacttattt attagtgatt atcaaaattc gcttccattg cgaaagtaat gtttcggaaa
    10801 attaaaatat tacatgttgt gaatgtattt ggaggtgctg ttggccgaat attggttgac
    10861 gtcatgccac atttagtaaa aaaatttgat gttaaagtgg tatcgataca atcaattgat
    10921 tattcggaac cattggtaca ggaatatatt aggaaatata acaatctgga atcgcttaaa
    10981 ttaaatcgtt tttctctgtt taaagcatat cataagctaa attgctattt taaaaagata
    11041 gatccagatt taattcattc tcatatggga agagctgata tttttacagc tatgtcaaaa
    11101 actaaaaata tgataataat gaataccctc cattgcgaaa aaaagaacca taatccattg
    11161 actcagatcg gttatagact aactgataga agggtcgatt ggagaatttg tatttcgcag
    11221 actgttgcga gaagttatta taatcatggt tttaaaagtc cttattcagt aatctataat
    11281 ccaattgata ttaatgggtt taccaaacca ataaatcgag aggaaataag aaataaccta
    11341 ggatttaagc ctaacgagca aattataatt aatgtcggtc ggctaattaa atgtaaaggt
    11401 cagaggtacc ttatagaagt aatgccgcat ttatgtcatt tattaccgga aataaaactt
    11461 ctcattgtgg gagatggccc tcagtataat agtttgaaaa aattgattat taataataac
    11521 ttagaaaata atgtatattt attaggctac cggcaagatg tacaaaatct gttaaaaata
    11581 tccgatctgt ttgtttattc agctcagtgg gaaggcttag gtatggcagt aatagaagca
    11641 atggcgacta gaattccagt tctcgctcac cccctgcctg ccctcaaaga atatatcatt
    11701 gataatcaca atggttattt ttgcaatatt cacttaaaag aatcgttcgc caataaaatt
    11761 tatgatatac ttactaattc tcatcataat tcatcaatag ctgaaaatgc tttcagaacg
    11821 gtgactgata tgttttctgc tgataaaatt agtaaacaat acattgaact ttatcggcaa
    11881 ttactatcaa atagataatt aataggacta tagattaagg ataagagtat aaagacagtc
    11941 attttagttt caaataccag ttggtattta tataattttc gtttaccact tttaaaaagt
    12001 ttaaaggaaa aaggatttaa agtaattaca attgcaccaa aagatgaata taccagccgt
    12061 ttaatatcaa atggatttac gcactatcat attccaattg atcgccgcgg attagctcta
    12121 tttccgaatt taattacaat actttccttc atatcacttt attaccgttt aaagcccaaa
    12181 atgattatcc atttcacgat taaaccagtg atatttggaa caatcaccgc ccggttttgc
    12241 actaaagcaa gaatagtaaa taacgtaact ggactagggt atatttttat tggagagact
    12301 tttttacata cattattgca gccatttgtg caatggatgt accgctgtgt tcttagacta
    12361 tcagatttat taatttttca aaacatagat gaccgtattt tttttatcaa gtctaaaata
    12421 attacggaaa agataccgcc aagtatttta atcagtggct ctggagttga tctggagaga
    12481 tttcaaagaa aaaataataa gaataaaaag tcagaagtaa attttattct agtagccagg
    12541 atgttatatg ataagggtat tcgggaatat attgaagccg ctaaaatcgt aaaaatatca
    12601 tttaaaaatg cagtgtttca cctccttggt gatattgata aaggcaatcc atcggcgatt
    12661 ccaattgaaa taattaacac ctggagtaaa cagggaattg taaattatta tggaatggtt
    12721 gaagatgtag ttccattcct ggaaaatgcc gatgtctttg ttttgccttc atatagagaa
    12781 ggattatcac gctcaatatt gaagctatgg ctatggaact cccaattatt accaccg
//
LOCUS       DAOOMU010000067        12702 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_5463_length_12702_cov_20.711315, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000067 DAOOMU010000000
VERSION     DAOOMU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12702
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5463_length_12702_cov_20.711315"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1166)
                     /gene="arsB"
                     /locus_tag="PLI55_07250"
     CDS             complement(33..1166)
                     /gene="arsB"
                     /locus_tag="PLI55_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPB00670.1"
                     /translation="MANERKLSVFEKYLTLWVLLCIGAGILLGKIAPGIAVKLDSLSI
                     YNVSIPIAICLFFMMYPIMVKIDFAEVVRAAKTPKPVALTLFINWAIKPFTMYLIASF
                     FLGHLFKGFLPGTEIIKTGQEVELWRSYISGAILLGIAPCTAMVLMWSYLAKGNDGLT
                     LVMVAINSLSMLVLYAPLGRFLLGVNAMPIPWQTILFSVMIYVALPLVAGYFSRKLIV
                     KSRGLEWFNTKFVHRLTPISMLALLITLVLLFSFKGEIIMENPLTIFWIAVPLFIQTI
                     FIFSLGYFVLARWLKLSYHDAAPAAMIGASNHFEVAIATATMLFGLSSGAALATVVGV
                     LIEVPVMLILVSICKRYCNLFGECHLDLPKCQPKYVTAEIKRA"
     gene            complement(1280..2872)
                     /locus_tag="PLI55_07255"
     CDS             complement(1280..2872)
                     /locus_tag="PLI55_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012488540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HPB00671.1"
                     /translation="MNTSRTGISGGSILKNLTENANLILKVISNEKGFKMGTLIFNRK
                     KYPTLSIGIILLVFFRSVLGQDYIPPDSTDMRNITSIELAQEMIPGWNVGNSLEAIGG
                     ETAWGNPQITQRLIDAVKAAGFNAIRIPVAWSKFSDESTFTIEESWLERVEEVVCYVL
                     SSGMYAIINEHWDNGWIQPTYADSAYVKNRLAAMWKQIAIYFRDYDDHLLFAGTNEVM
                     KEGNWGEPTKEYYTVQNIYNQVFVNTVRATGGRNYYRHLLVQGYRTDINCTVKYFVIP
                     QDVVPNRLMVEVHYYDPYNFTLNEDDKIIQWGKYATDASRTETWANESWADGQFNKMK
                     TNFIDKGYAVILGEYCATSRLNLGSAELNAQHAEYRRYYINYVTYSMVYHGLVPFYWD
                     NGFTGNHGSGIFNRADGTQAYPNLVKAIMDGVNLTVFPVGIKDEEKPKTAREFVLNQN
                     YPNPFNSQTKISYYLPRTGKVTIKVYDLNSCEVATLVRQELQKPGYYEVSFDASKLSS
                     GLYFYQLSSEHFTDTRKMLLLK"
     gene            complement(2925..5357)
                     /gene="galB"
                     /locus_tag="PLI55_07260"
     CDS             complement(2925..5357)
                     /gene="galB"
                     /locus_tag="PLI55_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HPB00672.1"
                     /translation="MKGIGLKIMLVLVASLLITQCSDSRKMVRQIDNFSQSWRFNLGD
                     LSDAERPEYDDSNWRTLDLPHDWSIEGEFSEEHPATPGGGALPGGIGWYRKTFTVSEG
                     NKDKSVFIDFDGVYQNSEVWINGHYLGKRPYGYSSFRYELTPYLNYGDENNIIAVKVD
                     NSQQPNSRWYSGSGIYRNVWLVVTDKVFVDHWGTFVTTPEVSSEAAKINIKTNVRNQT
                     GLDQEVILSTIIYDADGKLIARANTSQKIPKDTVIEIEQELSVKKPKLWSDERPYLYQ
                     AVTQISGAEKVWDDYSTSFGVRYFKFDPEKGFFLNGKPVKIKGVCNHHDLGCLGAAIN
                     TRAIERQLELLKSMGCNGIRTSHNPPAPELLDLCDRMGFIILDEAFDVWRVAKMKYDY
                     HLYFDEWHARDMADLVRRDRNHPSVFIWSIGNEVMEQWDKSNPSGMEIAQELANITRK
                     LDPTRPITAGCNDQSPENPVIKSNALDLIGYNYAHEKFADFPKTYPGKCFIATETHSV
                     IATRGSYDMPSDSIRVWPTRWDIPFTGGNPDFSCSAYDNCHVPWGSTHAETWSIVKKY
                     DFISGLYIWTGFDYLGEPTPYGWPARSSYFGIIDLAGFPKDSYYFYQSEWTEQPVLHI
                     FPHWNWQKGELIDVWAYTNYDEVELFLNDQSLGKKQKSDGVFQLVWRIEYTPGTLKAV
                     AHKKGSADLTKVIKTAGEPAGILLEPDRDVIKADGKDLSFITVKVVDSEGTTVPLADN
                     LINFEVEGPGFIAGVDNGSQISHEPFKANYRKAFHGLCLAVIQSQNTPGQITVKANSE
                     GLKPAIARIKVK"
     gene            complement(5375..8227)
                     /locus_tag="PLI55_07265"
     CDS             complement(5375..8227)
                     /locus_tag="PLI55_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPB00673.1"
                     /translation="MKISLRLALSLVLISGALLLADSRVQKLPNGVIIELAEEPAISP
                     RLIKIQVCDDDIIHVQATPESNFSERPSLIVEQAYANRLPESVNWTLQENEDTIILAT
                     AKLLVKISKTEGTVAFYKADGELILQERASGSKIFTAAEVMGEQTYHIQQFFDSPANE
                     AFYGLGQHQNGLMNYKGQSVDLWQYNIVVAVPFLISSRNYGILWDNNSRTKFGDIRDF
                     ESLATLKLYDKKGKPGGLSAEYFKDDGFKKLFTTRREPRIEHEFIDVNDPFPAGFDQV
                     ASVRWSGTIESAQTGIHKFRLYCCGYTKMWLNDSLVVDNWRQNWLPWTHFPEIFMEAG
                     KRYSIKIEWIHQGGYIGLKYLTPSPEEYANSLSLYSEVADQIDYYFIYGNSLDEVIHG
                     YRKITGQAPLMPKWAMGLWQCRQRYQTQDEVLSVVREFRQRQIPLDNIVQDWFYWEED
                     KWGDHEFDPARFPDPDGMVKELHEKLNAHIMISVWPKFYVGTKNYQQFLEKGWLYMRN
                     VEKNQRDWVGPGYVSTFYDPYRPEARDLYWQQINEKLFSKGFDAWWLDATEPDIHSNL
                     SPEETRLRMHPTAIGSAARYLNTYSLVHCKGIYENQRRTNPDQRVFILTRSAFAGQQR
                     FAAATWSGDVAARWYDLKTQIPAGLNFCLSGIPYWTTDVGGFAVESRFVNATGENLEE
                     WRELMTRWFQFATFCPLFRVHGEYPYREIYHVAPEDHPAYQAMLAYDHLRYRLMPYIY
                     SLAGMVTHNDYTIMRALIMDFSQDTKVQNVGDQFMFGPALLINPVTEYKARTRSVYLP
                     AGTGWYDLKTGKYFNGGRTIIAEAPYSDIPIFVKEGSIIPYGPEIQYADEKSADPIIL
                     YVYTGRDAEFELYEDEGVNYNYEKGQFATIPLVYSEKTQTLTIGKRQGEFPGMLKERT
                     FQIIRVSPDKMSGLGFTLEPDKIVKYDGQEITIKIK"
     gene            8804..10018
                     /locus_tag="PLI55_07270"
     CDS             8804..10018
                     /locus_tag="PLI55_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPB00674.1"
                     /translation="MSLSFAENLQNALEEFQTGTQRFEEINHHLAKNLAWKKEAYEHL
                     KNLLLHMVKIGASDINLGGPRTNDKVWYRVYGNLEPSQEVPTYTNDEVAAIALSILSD
                     QQKTILFNERNIDFAFAFSTDQSQKPYRFRGDIYYERNNLAINFRHIAEKIFPIESLE
                     IPEPIIKRLDLQHEKSGLFLVTGVTGSGKSTTLDSITDMNNHNNKAHIVIIGKPIEYV
                     HKSDKSLITHREVGDDVLSFRNGIIEALRQDPNIIIVGEMRDPATISAVLEATDSGHK
                     IFSTLHTSSTVESIHRIIAEFPPDEQERIRFRLADTLKVVISQKLVPNKEGKRTLAKE
                     ILSVDYSVQAAIRNNNITEIFQMITEGKNKGMFTLQQDLYRLVRNGTITTETALNYCN
                     NKKIMQQLLGFS"
     gene            10095..10652
                     /locus_tag="PLI55_07275"
     CDS             10095..10652
                     /locus_tag="PLI55_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPB00675.1"
                     /translation="MYIFGNLKAGFLNAWLGVLLIFLASWTILATDKKARRRLSNTSW
                     CSPREKKLVWSGTFLLFVILVFSIFVPLQLNTNWFYIGTAIFLVGYICLVISYFNYIK
                     TAGDKSKTKGFYNISRNPIYLFTSVALLGISIASASLIMLILVIIHFIISHFVILAEE
                     RHFLHIYKNTYKKYMKRVRRYFLFF"
     assembly_gap    10753..10852
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10957..>12702)
                     /locus_tag="PLI55_07280"
     CDS             complement(10957..>12702)
                     /locus_tag="PLI55_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB00676.1"
                     /translation="PPAGDIVNVEISAGDVGVHPDAEGNYHLAISPGIYEVTSSLDGY
                     TTVTLKDIVVEQNQVTEKIDMTLIDWAPISGNHYNMVVVARAKISGKIINGGNNNQIA
                     AFGSGGDEDCRGIGSWIEPSPPYDGYWYFTIRGNEIGEVIGFKIYLEELNAIETIIQT
                     ISFEDNATIGTPDEPLELRNFTKQEFSLQKDWNWVSFNIHLDNPLIDSVFSSLTSSIF
                     QIKSQNKSATFYRAQNKWMGDLTQIDDGKTYLIKMDQAVEKFSVVGTPINVSTPIELI
                     EGWNWIAYYPQNSMAVNSALQSIMPNAFQVKSQTQSATYFDPPSTWVGDLSLMEPNQG
                     YKLKVNSPSTLIYPENLLAKSLSKITDIADSPDWHQITGTQYNMVLVASVKLDNEKFD
                     SSNGNLVGAFGPGGEEDCRCVATWQKPVDDSDGFWYFTIVGDEHNEEISFKLYDGKND
                     RIINCNQTIKFVVDSTIGMPPEFFIITANTSDILNPGIQTKFKLAQNYPNPFNSTTQI
                     KFSLEKQSNVNITVYDLTGQKVRTLLDAKLNLGEYRVVWDGRDDWGMPVSSGIYLYRM
                     KADNYSELRKMILSK"
BASE COUNT         3707 a         2860 c         2432 g         3603 t
ORIGIN      
        1 cgcgggtact cttcactaat aatactcttt cattaggctc tttttatctc ggcagtcaca
       61 tatttgggtt ggcactttgg caaatctaaa tggcattctc caaataaatt acagtatcgc
      121 ttgcaaattg aaacgagtat tagcattacc ggaacttcaa tcaaaacacc gactacggtt
      181 gctaacgccg caccagaaga caatccaaaa agcatagtgg cggtcgcaat tgccacttca
      241 aaatgattcg aagcaccaat catcgcggcc ggagcggcat catgatagga aagttttaac
      301 catcgtgcta aaacgaagta tcccaaacta aaaataaata tggtttggat aaaaagggga
      361 actgcaatcc agaatatcgt cagaggattt tccattataa tttctccctt gaaagagaat
      421 aaaaggacca atgtaataag aagtgccaac atacttattg gtgtgagacg atgcacaaac
      481 ttcgtattaa accattctaa acctctggac ttgacaatca acttccgcga aaagtaccct
      541 gcgaccaatg gcaaggctac ataaatcata accgaaaaca atattgtctg ccagggtatg
      601 ggcattgcat tgacaccaag taaaaatctc ccaagagggg cataaagtac gagcattgat
      661 aaagagttta tagcaaccat aaccaaagta agtccatcat tgccttttgc cagataactc
      721 cacatcaata ccatggcggt acagggggca attcccaaaa gaattgcacc ggagatataa
      781 cttctccata actcaacttc ttgcccggtc ttaataatct cggttccagg taagaatccc
      841 ttaaacagat gtcctagaaa aaatgaagca atcagataca tagtaaatgg cttgattgcc
      901 caattgatga acaatgttaa cgccacaggc ttaggcgttt ttgccgctct aactacctct
      961 gcaaaatcta tctttaccat aattggatac atcataaaaa ataggcagat agcaatggga
     1021 atggagacgt tatatattga aagagaatcc aatttgaccg caatcccggg tgctattttc
     1081 cctaatagga tacctgcgcc gatgcaaagt aaaacccaga gagttaagta cttttcaaaa
     1141 acagaaagtt ttctttcatt agccattttt atttaccttc tttttggtgg cattttattt
     1201 tccattcgaa tcaattatac aaacaaaatg attccaatat caaaaatttt gcagacaact
     1261 cgaattattt agagcggact catttgagta aaagcatctt tctggtatca gtgaaatgtt
     1321 cgctggacag ctgataaaaa tataagccgc tggataattt cgaggcgtca aatgacactt
     1381 cataataacc aggcttttgt aactcttgtc gtacgagagt cgctacctca caactgttca
     1441 aatcatagac tttgatggta acttttccgg tgcgcggcaa atagtaggaa attttggttt
     1501 gcgaattgaa gggattggga taattttggt tgagcacaaa ttcacgagcg gttttcggct
     1561 tttcttcatc tttaatgcct accggaaaca ccgtcaaatt gacgccgtcc ataatggctt
     1621 tcaccagatt cggataagcc tgtgtgccgt cggcccgatt aaaaataccc gagccatgat
     1681 tgccggtaaa cccattatcc cagtaaaagg gcaccagacc gtgatagacc atcgagtaag
     1741 taacgtagtt aatataatag cgccgatatt cggcatgctg ggcattgagt tcggcactgc
     1801 cgagattcaa ccttgacgtc gcgcaatatt cacctaaaat taccgcataa cctttgtcaa
     1861 taaaattggt tttcatttta ttgaactgtc cgtccgccca ggattcgtta gcccaagtct
     1921 cagttcgcga ggcatcggta gcatactttc cccattgaat aattttatca tcttcattta
     1981 aggtgaaatt ataggggtca taataatgca cttccaccat caaacgattg ggaaccacgt
     2041 cttgaggaat aacaaaatat ttgacagtac aattgatatc agtcctataa ccctgaacca
     2101 atagatggcg ataataattg cgcccgccgg tggcccgaac ggtattgacg aatacctgat
     2161 tatatatatt ctggacggta taatattctt tggtaggctc gccccaattg ccctctttca
     2221 ttacttcgtt ggtgccggca aacagaaggt gatcatcata gtcgcggaaa tagatggcaa
     2281 tctgtttcca catcgccgcc aaccgatttt ttacgtatgc cgaatcggca taagtaggtt
     2341 gaatccagcc attgtcccag tgttcgttga taatggcata catcccgcta ctcaaaacgt
     2401 aacaaaccac ttcttcgact cgctccagcc aggattcttc gatggtaaaa gtagattcgt
     2461 cggaaaattt gctccaggca acaggaatcc ggatggcatt gaagccggcg gctttcacgg
     2521 cgtcaatcaa cctctgagtt atctgcggat tgccccaagc agtttcgcca ccgatggctt
     2581 ccagtgaatt tccgacattc cagccgggaa tcatttcttg agccaattca atggaagtaa
     2641 tattgcgcat atcggttgag tcgggtggga tataatcctg ccccaaaacc gacctaaaaa
     2701 ataccagcag gataatccca attgaaagag ttgggtattt tttgcgatta aatattaaag
     2761 tccccatttt gaaacccttt tcattagaaa taacttttaa tatcagattg gcgttttcag
     2821 ttaggttttt taaaatggac ccgcctgaaa tgccggttcg agatgtattc atactcaatc
     2881 atctcggagt ttaaatagca tctcaatgcg ccactaaatt tttctcattt taccttgatc
     2941 ctggcaatag ctggctttaa gccttcggaa ttagctttga cagtaatttg ccctggcgtg
     3001 ttttgggact ggataaccgc taaacacaat ccgtgaaaag cttttctata gttggcttta
     3061 aacggttcgt ggctgatttg actgccatta tcaacgccgg cgataaagcc cggtccttca
     3121 acctcaaaat taattagatt atcggcaagc ggtacagtcg tgccttcact atcaacgact
     3181 tttactgtaa taaatgacag gtctttacca tcagctttta taacatcgcg atcgggctcg
     3241 agtaaaatgc cagccggttc accggcagtt ttaattactt tagttaaatc agcagagcct
     3301 tttttgtgtg ccacagcttt cagggtgccg ggtgtgtact ctattcgcca aaccaattga
     3361 aaaacaccgt cgcttttttg ctttttaccc aacgattgat cattgaggaa aagttcgacc
     3421 tcatcataat tcgtataagc ccaaacgtca attaattcgc ctttctgcca gttccagtgc
     3481 ggaaaaatgt gtaataccgg ttgctcagtc cattcactct ggtaaaaata ataactgtct
     3541 ttagggaacc ccgccaaatc gataattcca aaataggaac tacgcgccgg ccatccgtac
     3601 ggagtcggct cgccgagata atcaaaaccg gtccagatat ataatcccga aatgaaatca
     3661 tactttttca cgattgacca ggtttcggca tgagtcgaac cccagggcac gtgacaatta
     3721 tcataagccg agcatgaaaa gtccggattg ccgccagtaa aaggaatatc ccagcgcgtc
     3781 ggccagacgc gaatgctatc cgacggcata tcataactgc cacgcgtcgc tataacggag
     3841 tgagtttcgg tagcgataaa gcacttgccc ggataggttt tcggaaagtc ggcaaatttt
     3901 tcatgcgcat aattatagcc aatcaaatcc agtgcattag atttaatgac aggattttcg
     3961 ggactctgat cattacagcc agctgtgata ggacgcgtgg gatcgagctt tctggtaata
     4021 ttagccaatt cctgggcaat ttccatgccg ctgggattac ttttatccca ctgttccata
     4081 acctcgttcc cgatactcca gataaaaacg ctggggtgat tgcgatcgcg tcgaactaaa
     4141 tcggccatat cgcgcgcatg ccattcgtcg aaataaagat gataatcgta tttcattttc
     4201 gctactcgcc aaacatcgaa agcttcatct aatatgataa aacccatccg atcacacaaa
     4261 tccagcaatt ccggcgccgg cggattgtgg gaagtgcgaa tcccattaca acccatagac
     4321 tttaataatt ccagttgccg ctcgattgca cgcgtgttaa ttgcggcacc cagacagccg
     4381 agatcatgat gattgcatac gcctttaatt ttcactggct taccattcag aaaaaatccc
     4441 ttttccggat cgaatttgaa ataacgaact ccaaaagaag tcgaataatc atcccagact
     4501 ttttccgctc cgctaatttg agtcacagcc tggtataaat aaggacgctc atccgaccac
     4561 aacttgggtt ttttcactga caattcctgt tcgatttcaa tcacggtatc ctttggtatc
     4621 ttttgggaag tattggctct ggcaatcaac ttcccatcag catcatagat aatggtactc
     4681 aggataactt cctggtcgag accggtctga tttctaacgt tggtttttat attgattttg
     4741 gcggcttcgc tactaacctc cggcgtggta acaaatgtac cccaatgatc tacgaatact
     4801 ttatcggtga ctactaacca gacgtttcgg taaatcccgg atcccgaata ccaacgcgaa
     4861 ttgggttgct gggaattatc aacttttaca gcgataatat tattttcgtc gccatagttg
     4921 agataaggtg tcaattcgta tcgaaaagaa ctatagccgt acggtctttt ccccaaatag
     4981 tggccattta tccagacctc gctattttgg taaacgccgt caaaatcaat gaaaacactc
     5041 ttatctttat ttccctcgga aacagtaaaa gtcttgcgat accatccaat tccacccggt
     5101 aaagcaccac cacccggggt tgcgggatgc tcctcactaa attcaccttc gatactccaa
     5161 tcatggggca aatctaaagt acgccaattg gaatcgtcat attcgggtct ttcggcatca
     5221 cttaaatctc ctagattaaa tcgccaactt tggctaaaat tatcaatctg tctgaccatc
     5281 ttgcggctat cagagcattg agtaatgagc agtgatgcaa caagcaccaa cattattttt
     5341 aatcctatcc ctttcataaa tacccccaaa aatattattt gatttttatc gttatttcct
     5401 ggccatcata ttttactatt ttatccggtt cgagagtaaa gccaagtccg ctcattttat
     5461 ccgggctaac tcggataatt tgaaatgttc tttccttcaa cattcccgga aattcaccct
     5521 gacgctttcc aatcgtcaat gtttgagttt tctcagaata aaccaatgga attgtggcaa
     5581 attgcccttt ttcataatta taattgactc cttcgtcctc gtaaagttca aactcggcat
     5641 cccgaccggt atagacatac aaaataatcg gatcagcgga tttttcatcg gcgtactgaa
     5701 tttccggtcc gtaaggaata attgaacctt ctttcacgaa aatcggtata tcgctataag
     5761 gggcctcggc tataatcgtc cgaccgccgt tgaaatattt cccggttttc aaatcatacc
     5821 aaccagtacc agccggtaaa tagaccgagc gtgtgcgggc tttatattcc gtcaccggat
     5881 taatgagcaa agccggaccg aacataaatt gatcgccgac attttgaact tttgtatcct
     5941 gactgaaatc cattatgagc gctcgcataa tcgtgtaatc gttatgcgtt accataccag
     6001 ccagcgaata aatatagggc attaagcgat agcgcaagtg atcgtaggct aacattgcct
     6061 gataggcggg gtgatcttct ggcgcaacgt gataaatttc acggtaggga tattctccat
     6121 gaacccggaa aagcggacaa aaggtggcaa actgaaacca gcgggtcatt agctcgcgcc
     6181 attcttccag attttcgccg gtggcgttga caaagcgaga ttcgaccgca aagccaccga
     6241 catccgttgt ccaataggga attccggata gacaaaaatt caaaccagct ggaatttgag
     6301 ttttcaaatc ataccagcgg gcggcaacat ccccgctcca ggtcgcggcg gcaaaccgct
     6361 gttgtccggc gaaagccgaa cgagttagaa taaaaacccg ttgatcggga ttagttcggc
     6421 gctgattttc ataaattccc ttgcaatgaa ctaacgaata agtattcaaa tagcgagcgg
     6481 cgctaccgat ggcagttgga tgcatccgca agcgcgtttc ttccggcgac aaattggaat
     6541 gaatatccgg ttcggtagca tccagccacc aggcatcaaa ccccttactg aataattttt
     6601 cattgatctg ttgccagtat aaatctctgg cttccggacg gtaaggatcg taaaatgtgg
     6661 aaacgtagcc gggtccgacc caatctctct ggttcttttc gacattacgc atatatagcc
     6721 agcccttttc cagaaattgc tggtaatttt tcgtcccgac gtaaaacttt ggccagaccg
     6781 aaatcataat gtgagcattt agtttttcat gcagttcttt gaccataccg tctggatcgg
     6841 gaaagcgagc tgggtcaaat tcgtggtcgc cccacttatc ctcttcccaa tagaaccaat
     6901 cctgaacaat attgtccagc ggaatctggc gctggcgaaa ttcccgcaca accgaaagca
     6961 cctcgtcctg agtctgataa cgctggcgac attgccacaa acccatcgcc cattttggca
     7021 tcagcggcgc ttgcccagta attttgcggt agccgtgaat cacttcatct aaactgttac
     7081 cataaataaa gtaatagtca atctggtcag caacctccga ataaagcgaa aggctattag
     7141 catactcctc tgggctggga gtcagatact tgaggccaat atatccacct tgatgaatcc
     7201 attcaatttt aattgaatag cgcttcccgg cttccataaa aatttccgga aaatgcgtcc
     7261 agggcaacca attctgacgc caattatcca ccactaaaga gtcattcagc cacattttag
     7321 tatagccgca acaatagagt cgaaacttat gaatgccggt ttgggcggat tcgattgtcc
     7381 cgctccagcg cacactcgct acttggtcga atccagctgg gaatggatcg ttgacgtcaa
     7441 taaactcgtg ttcgatgcga ggttcgcggc gagttgtaaa tagtttttta aatccgtcat
     7501 ctttaaaata ttcggcagat agaccgcccg gtttgccttt cttgtcatat aattttaatg
     7561 tggctaatga ttcaaagtcg cgaatgtcac cgaatttggt gcgggaatta ttgtcccaca
     7621 aaataccata attgcggctg gaaatcagaa atggcacggc taccacgata ttatattgcc
     7681 agagatcaac cgactgtcct ttataattca tcaatccgtt ctggtgttgt cccaatccgt
     7741 aaaaggcttc attggcaggc gaatcgaaaa actgttgaat atgataagtc tgttcaccca
     7801 tcacttccgc cgcagtaaaa attttactgc cgctagctct ctcctgtaaa attagttcgc
     7861 cgtctgcttt atagaaagca accgtacctt cagttttcga tattttcacc agcagtttgg
     7921 cagttgccag aatgattgta tcctcgtttt cttgtaatgt ccaattaacc gattcaggca
     7981 accgattggc ataagcctgc tccactatca gactgggacg ctcagaaaaa ttggactcgg
     8041 gtgtagcctg gacgtgaata atgtcatcgt cgcagacctg aatctttatc agtctgggcg
     8101 agattgcagg ttcttccgcc agctcaataa tgacaccatt gggcagtttt tgtactcggg
     8161 aatcggcaag caaaagtgcg ccggaaatta acaccagcga tagagccagc cttaagctaa
     8221 ttttcatttg ttcgcctctt atttgtaaaa tttcaaaagc cgtatatcca tgctccaaat
     8281 ataatcaatt aatatggctg atggcaaaat tttaatcgtt ccgagaataa tttactaatc
     8341 atgttcgcgc ctgaactaaa gcaaattggc aaatgattga aaggaacttt aacaacagag
     8401 gcacgagaaa taaggctaag gaaacaagtt actggtttca agtcaacgcc gaaatggcga
     8461 gtcaagctgc gcctttagca ttttataatt ttcaccattt aaaaagattg cctcaagggc
     8521 gaggctaagg gcggtaagaa attaagtcct aataagtgct actaagtact tttaagtgtt
     8581 tataagaata tataattcgt cacaacttta taactttaaa aactcatgta tttatgcagt
     8641 catgcagtca tgcgaaataa aaaactgtat tttccgtaac ttttttcatc cctattttct
     8701 tcattggaaa atgaaaattt taaattaaaa tatacagaaa tgcgattaac cagcctgatg
     8761 aacactaaag aaattttcac ctaaaaaatc aagaaggaac cttatgagtc tgtcatttgc
     8821 cgagaacttg caaaacgcat tggaagaatt tcagaccgga actcagcggt ttgaggaaat
     8881 caaccatcac cttgcaaaaa atctcgcctg gaaaaaagaa gcctatgaac atctaaaaaa
     8941 tctattgctt cacatggtaa aaatcggcgc ttctgatata aacctcggcg gtcccagaac
     9001 caatgacaag gtttggtacc gggtttatgg aaatttagag ccgagccagg aggtacctac
     9061 ctacactaat gacgaggttg ccgcaatcgc attaagcatt ttatccgacc aacagaaaac
     9121 gattctcttt aacgaaagaa acattgattt tgcctttgcc ttttctaccg accaaagcca
     9181 aaaaccatac cggtttcgcg gcgacattta ctacgagaga aataacctgg cgatcaattt
     9241 ccgacacatt gctgaaaaaa tattccctat agaaagttta gagattcccg aaccaattat
     9301 caaaaggctc gacctccagc atgaaaaatc gggcttattc ttagttaccg gcgtaaccgg
     9361 ttcgggcaaa agcaccactc tggattctat aaccgatatg aataatcata ataacaaagc
     9421 ccatattgtc atcattggta agccgatcga atatgtccat aaatccgata agtcattgat
     9481 cactcatcgc gaagtaggcg atgacgtatt aagttttcgt aatggcatta tcgaagcgct
     9541 gaggcaggat cctaacatta tcatcgtcgg cgaaatgcgc gatccggcta ccatatcggc
     9601 agttctggaa gcgactgata gcggtcacaa gattttctcc accctccaca ccagctcgac
     9661 ggtcgaaagc atccaccgaa ttattgccga attcccgccg gacgaacaag aaaggattag
     9721 attccgcctc gctgatacat taaaagtggt aatttcgcaa aaattggtgc ccaataaaga
     9781 aggtaaaaga accctggcta aagaaattct ctccgtggat tattccgttc aagccgccat
     9841 tagaaacaat aacattaccg aaatcttcca gatgattacc gaaggtaaaa ataaaggcat
     9901 gttcacgctt caacaagatt tataccggct ggtgaggaac ggtaccatca ccacggaaac
     9961 cgccctgaac tactgtaaca acaaaaagat aatgcagcag ttactgggtt tttcataaaa
    10021 agttattgat gaatttctcc gcgctatgct ctttgcaaag aattcagcca taagtaagaa
    10081 aaaaagggta aaaaatgtat atatttggaa atcttaaagc cggctttttg aatgcttggc
    10141 tgggagtttt gctaatattc ctggcgagtt ggactatctt agccactgac aagaaagcac
    10201 gcagacgtct atcgaacact tcctggtgca gtccccggga aaaaaagtta gtttggtccg
    10261 gaacattctt attatttgta attcttgtat tctcgatatt tgttccgctt caactcaata
    10321 ccaattggtt ttacattgga acagccatat ttcttgtggg atatatttgt ttggtgatca
    10381 gctattttaa ttatatcaaa actgccggtg ataaatctaa gaccaaggga ttttataata
    10441 tctcccgaaa tcccatctat ctttttacct ctgtcgcact tctcggaatt agtattgcct
    10501 cagcctcgct cattatgctg atcttagtaa ttatccattt tatcatttcc cattttgtaa
    10561 tcttagcaga ggaacgccat tttcttcata tttacaaaaa tacttataaa aaatatatga
    10621 agagagttcg ccgctatttt ctattttttt agaatctatt catattcaat cccggtcggg
    10681 agagtttagc agatagataa attaaatatt taatcgtgat acataatctt caactccgcc
    10741 aggagttgaa ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaccaccca
    10861 aaccccgcag gggtttaacc catctaaaag aaacacattc gttcattagc acttctacca
    10921 cacggcttgc ttatgttctg actttttaag cagactctac ttgcttaaaa tcatcttcct
    10981 tagttcagaa taattatcgg ctttcattct gtataaataa ataccggatg aaactggcat
    11041 tccccagtca tcccttccat cccagacaac tctatactcg cccaaattta gtttcgcatc
    11101 taatagcgtt ctcacttttt gaccagttag atcatacacc gtgatgttca cattactctg
    11161 cttttccagc gaaaatttta tctgggtagt agaattgaat ggattaggat aattttgagc
    11221 taacttaaat ttagtttgga ttcctgggtt tagtatatca ctcgtattgg ctgtaatgat
    11281 gaaaaattca ggaggcatgc ctatggtgct atctactaca aattttattg tctgattgca
    11341 attaataatt ctatcatttt taccatcgta caacttaaaa ctaatttctt cgttatgctc
    11401 atcacccact atggtaaaat accaaaatcc atcactatca tccacgggct tttgccaggt
    11461 ggctacacat ctgcaatctt cttcaccgcc gggaccgaat gccccgacta agttaccgtt
    11521 actgctatca aatttctcat tatccaattt cacacttgct acaagaacca tattatattg
    11581 agtgcctgta atctgatgcc aatcgggtga gtcggcaata tccgttattt tacttaagga
    11641 tttagccaaa agattttccg gataaattaa agtggaagga ctgtttactt ttaatttata
    11701 cccttgattt ggctccatca gactgaggtc gcctacccaa gtactgggag gatcgaagta
    11761 cgtcgccgat tgagtttggc tttttacttg aaaagcattg ggcataatac tctgcaaagc
    11821 cgaatttact gccatagaat tttgtggata ataggcaatc caattccatc cttcgattag
    11881 ttcgattgga gtggatacat ttataggtgt acctactaca gaaaattttt ctactgcctg
    11941 atccatcttg ataagatagg ttttcccgtc atcaatttgg gtcaaatcac ccatccattt
    12001 attctgcgct cgataaaagg tcgcggattt attttgactc tttatttgaa aaatgctact
    12061 ggttaaactg gaaaaaactg aatctatcag aggattatca agatgaatat taaatgatac
    12121 ccaattccaa tctttttgta aagaaaattc ttgctttgtg aaatttctta attcgagcgg
    12181 ttcatcagga gtaccgatgg tcgcattatc ctcgaaagaa atagtctgga taattgtttc
    12241 gattgcattg agttcttcca agtaaatttt aaaaccaatc acttcgccta tttcattccc
    12301 ccgaatagta aaataccaat agccatcgta gggaggtgaa ggctcgatcc aactccctat
    12361 ccctcgacaa tcttcatcgc ctcccgaacc aaaagccgct atttgattat tatttccgcc
    12421 attgataatt ttgccgctga tttttgctct cgctacaacc accatattgt aatgattgcc
    12481 gctaattggc gcccaatcaa tcagcgtcat atcaattttt tcggtaacct ggttttgctc
    12541 aacaacaata tcctttaatg taaccgtggt atagccgtcc aggctggagg taacttcata
    12601 tatcccggga gaaatagcta gatgataatt gccctcagca tcgggatgaa ccccaacgtc
    12661 gcccgccgat atttcaacat ttacgatatc acctgccggt gg
//
LOCUS       DAOOMU010000068        12200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_5769_length_12200_cov_20.802800, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000068 DAOOMU010000000
VERSION     DAOOMU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5769_length_12200_cov_20.802800"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..734)
                     /locus_tag="PLI55_07285"
     CDS             complement(3..734)
                     /locus_tag="PLI55_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPB00677.1"
                     /translation="MRFAIIADIHGNLAALEAVLADIDQQQIDRIVDLGDFVGYGPEP
                     EDVTQLLRELQIAGIVGNHEVALTDDELLNYFSVDAFESIIITRQLISEQTLEYIKTL
                     PTFLTVDNLRFVHGTPPDSYKDYINYYGEHELRKAFASTPEWITFVGHTHQLGLYESD
                     AENKNIHTSILKKGCRQLSRQSKYIINIGSVGQPRDENNSAKYVIFDDETDALEVRAV
                     PYDVEHTIRLIQDIGLPESNAYRLL"
     gene            complement(1273..2166)
                     /locus_tag="PLI55_07290"
     CDS             complement(1273..2166)
                     /locus_tag="PLI55_07290"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HPB00678.1"
                     /translation="MFLILINQNIGEEKMIYHKLTPDEEKVIIHKDTERPFSGKYYDF
                     YEDGIYTCKRCDAPLYRSRDKFDAGCGWPSFDDEITGAVMRVLDQDGRRTEIVCANCG
                     AHLGHFFTGENFTAKNNRHCVNSISLNFIPAKDTSETALAIYAAGCFWGVEYYFKQAP
                     GVLATRVGYTGGHTTNPTYKQVCSGKTGHKEAIEVTYDPTVTSYEELTKLYFEIHDFT
                     QTDGQGPDRGEQYLSYIFYLDEFQKKVAEDLIALLKRKGFAVATKIEKAGAFYPAEDY
                     HQDYYTKTGKKPYCHFRKKIF"
     assembly_gap    2173..2272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2392..2886
                     /locus_tag="PLI55_07295"
     CDS             2392..2886
                     /locus_tag="PLI55_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPB00679.1"
                     /translation="MKYHYCYHCGTPTTSLEMEGYRRAFCPQCQLILYENPLPTVVII
                     AYNEQNEIVLIRRAVEPGRGGWSLPGGFIEIGETAAQAAVRELCEETGLSATELKLVD
                     VGTHLNGFYGDILMIGYATPVQEDLIRPGSDAAEAAWFKIDDHPSLVFPFHEKLIENW
                     RMIK"
     gene            2889..3956
                     /gene="mnmA"
                     /locus_tag="PLI55_07300"
     CDS             2889..3956
                     /gene="mnmA"
                     /locus_tag="PLI55_07300"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPB00680.1"
                     /translation="MVTKKQKVLVALSGGVDSAVAAWVLCQEGYEPVGITFRMWRMKE
                     DIAQAKAIDRARQVCEILKIPHIIFDLNAEFEEQVVKPFVNEYFQGLTPNPCVNCNRV
                     IKWHNLQKMADQYGIEKIATGHYARLKYNSANKRYQILKGVDSTKDQSYALWQIPQTA
                     LKRTLFPLGEMTKNAVKTIAVDHNLIVGKITESQNICFIPEGDYRSFLKEYAPQKVQT
                     IGRGELVDERGRVLGWHNGFYNFTIGQRKGFRVGFKERRYVKAINAPKNRVVIAPDKS
                     LFSSKMIIKNVNWVGISAVAEFDGIIKIRYNHPGAMGHGKRINDNEYEITFIEPQRAI
                     CPGQSAVVYQDDCLLMGGIIQ"
     gene            4023..4823
                     /gene="panB"
                     /locus_tag="PLI55_07305"
     CDS             4023..4823
                     /gene="panB"
                     /locus_tag="PLI55_07305"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPB00681.1"
                     /translation="MQDKVRVSTILEMKQKGVKITSLTAYDFTMAHLMDESGVDIILV
                     GDSCGNVCAGYETTLPVTMDEMVYHVQCVRRGVKRALLVADMPFMSYQESVEQALHNA
                     GRFMKECGAEAVKLEGGEPICPVVEKMVEIGIPVMGHLGFTPQSVHKFGGFGVRGKET
                     EEAKYIEKSAFALQDAGAFAIVLEKIPAKLAADVTAQLRIPTIGIGAGKECDGQVLVS
                     YDMLGLFDQVKYKFVRRYANLADEIRRATTAFGNDVRNGNFPDESESY"
     gene            4847..>5103
                     /locus_tag="PLI55_07310"
     CDS             4847..>5103
                     /locus_tag="PLI55_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020059576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPB00682.1"
                     /translation="MQILTKIEDIRQELLVIRTSAKKIALVPTMGYLHEGHLSLVDVA
                     KANADVVVMSIFVNPTQFAPNEDLMRYPRDIERDERLARERG"
     assembly_gap    5104..5203
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5204..5464
                     /locus_tag="PLI55_07315"
     CDS             <5204..5464
                     /locus_tag="PLI55_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPB00683.1"
                     /translation="QQAPIIFKALQAARENVKNGLTDANQVRDQITKMIQTSPLARID
                     YVEIIDDQTLTPVETVKPGTFAAVAVYYGKTRLIDNIYLKER"
     gene            5550..6029
                     /locus_tag="PLI55_07320"
     CDS             5550..6029
                     /locus_tag="PLI55_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00684.1"
                     /translation="MKRNFLTVTMLAIFLGTSGFAQLKTQIQRSPTIGEAIHLPGISS
                     AISNYSLFDPSRFKMQQSYSLSYSTFGNQSMSVGVYQNAMSFLLSNKFLLNTRFGFYH
                     NPLQLGTFNATSNNMLDNLIFGADLIYRPKENATFMIRFDRRPYYYPYEYYNPYLGY"
     gene            6032..6571
                     /locus_tag="PLI55_07325"
     CDS             6032..6571
                     /locus_tag="PLI55_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPB00685.1"
                     /translation="MARSRNRRKRPTSRKNKRRAVDLQKWFLNLAIFSISVVIVGFIF
                     SMGKRFNNPNEKLTMSQVNVIPPDERPVPYIVIEVLNGTDVPGLAQKFTNFLRQNGYD
                     VISTGNADYSGYEKTMLITRTADVTKLREVNGTLLLDSDQLFQKIDSTLQVDLTLIVG
                     KDYQRLPVYSKIIAIGETF"
     gene            6575..6958
                     /gene="rsfS"
                     /locus_tag="PLI55_07330"
     CDS             6575..6958
                     /gene="rsfS"
                     /locus_tag="PLI55_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPB00686.1"
                     /translation="MKTSQNTKDKNQSYPLARQIADIAVDKKARDVVIIDLRPLTSIT
                     DYFVICTGAVGQHVKAIVDEIDDRLRPAEKPWHIEGYQHLSWVLMDYVNVVVHVFNPE
                     ARAYYNLERLWADAATEMISDDSAD"
     gene            7009..7920
                     /gene="queG"
                     /locus_tag="PLI55_07335"
     CDS             7009..7920
                     /gene="queG"
                     /locus_tag="PLI55_07335"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPB00687.1"
                     /translation="MSLTAEIIRLAENSGLARVGISPPFLDYKYITFFQQWIDAGFLG
                     TMSWMRKYYDFQNYQKTYFSWAKSVLVVADNYFQNVEWPTGIPKIARYAWGKDYHKVV
                     KQKLAKVSREINLLEPATVSRIYVDSGPVMQKIFAWQAGLGWVGKNNLLIVPDLGSYC
                     FIGMIFLNLELHFNEPQSNLCGDCQLCRDACPNQALVDEHLLDARLCTAYLTIEKDGE
                     FSMAEKENLRGWIYGCDTCQKICPYNQKWKRPGDARYSEYYSEFSKSLTDWLKVTEDE
                     FGEIFRDSPIRRIGFANWRRNLLTLRK"
     gene            8000..>8590
                     /gene="argS"
                     /locus_tag="PLI55_07340"
     CDS             8000..>8590
                     /gene="argS"
                     /locus_tag="PLI55_07340"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPB00688.1"
                     /translation="MIEHIRGLLEKALQQNDLDYREPIQIERPKQETFGHYTTNLALA
                     VAKIQRQSPLEIAEKLVATLPQSEYIERVEYRAPGFINFFLSPRVLQQTVAQIILAAE
                     NYGKLDIGKNQRVQVEFVSANPTGPLTVGHGRQAVLGDTVARILEWVGYRVDREYYYN
                     DAGRQMRMLGHSVYWRYRQLLGWKGEFPEDHYQGEYI"
     assembly_gap    8591..8690
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8691..9472
                     /locus_tag="PLI55_07345"
     CDS             <8691..9472
                     /locus_tag="PLI55_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HPB00689.1"
                     /translation="LYGGTDDRVLWKSVVDEATYRLPDIAYHVTKFERGYDLIIDIFG
                     ADHHATYPDVLAGLRALGYDTSRVKVLIHQFVTLIKGGEKVKMSTRKANYVTLDELID
                     DVGRDVARYFFLMRGMKSHLNFDLDLAKTESEENPVFYLQYAHARICSILRNAATKNL
                     AVGNDFDMTLLKEEETLSLLNALSEFKEVIEICVSTLEPQHLTTYLQKVATAFHKFYT
                     VHRVISKDWELSQARLALVKAVQIVMANGLALLGISAPERM"
     gene            9583..9882
                     /gene="gatC"
                     /locus_tag="PLI55_07350"
     CDS             9583..9882
                     /gene="gatC"
                     /locus_tag="PLI55_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPB00690.1"
                     /translation="MRVDLKTAEKIADLARLTLSEAELERYAAQMDQILEYIEQLQNL
                     DTSNVKPLSHVQEITNVFRNDEVKPSLPKEAVFANAPQEKAGYFVVPKVIKDINP"
     gene            9879..>10150
                     /locus_tag="PLI55_07355"
     CDS             9879..>10150
                     /locus_tag="PLI55_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPB00691.1"
                     /translation="MKVLGVIPARFAATRLPGKPLVSLHGKTLIQRVYENARNSQLIN
                     QLIVATDDQRIFEHVKSFGGECRMTPVELASGTDRCALVARDNEAD"
     assembly_gap    10151..10250
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10251..11798
                     /locus_tag="PLI55_07360"
     CDS             <10251..11798
                     /locus_tag="PLI55_07360"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPB00692.1"
                     /translation="SRGFKVTIQKFDPYINIDPGTMSPFQHGEVFVLEDGSETDLDLG
                     HYERFIDENMNTRNSNTSGKIYYEVINRERRGDYLGATVQVIPHITDEIKRRITAVNV
                     DSAYDVVICEIGGTVGDIESQPFLEAIRQFRFESGRGNCINVHLTLVPYIETSGEIKT
                     KPTQHSVMRLREIGIQPDILLCRSKFHLDESIHKKIALFCNVMPEVVIDTIDAETIYE
                     IPLIFDQQKLGDQITDLLDLPRRKPDLKDLEKFVQAIKNPKDEVNIAICGKYTQLHDA
                     YKSIIESFNHAGAENNTRVNIVWIESEDLTNDCETVNKLKNVDGILVPGGFGERGVKG
                     KINAIKLARENGIPFLGICLGLQCAVVEFARNVCGWKDADSTEFNENTQHPVIDLMEE
                     QKRVFNKGGTMRLGAYTAEVIPGTLAYEIYKNKQISERHRHRYEVNNTYLAEMVKAGL
                     VVGAVNKELNLVEMIELKNHPWFVACQFHPEMKSRINKAHPIFREFVRAARKYHNQKA
                     ENARSQS"
     gene            11779..>12200
                     /locus_tag="PLI55_07365"
     CDS             11779..>12200
                     /locus_tag="PLI55_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPB00693.1"
                     /translation="MPEVKVKNIAIGNHQPLAFILGPCVIESRDHALRMAEKLKALSE
                     ELDFPLIYKSSFDKANRSSIESFRGPGLEAGLAILAEVKQQFGLPILTDIHEPFQAEI
                     VAKVVDVLQIPAFLCRQTDLILAAGRTGKVVNVKKGQF"
BASE COUNT         3384 a         2359 c         2702 g         3355 t
ORIGIN      
        1 gtttataata aacgataagc atttgattcc ggtagtccaa tatcctggat caaccgaata
       61 gtatgctcga cgtcgtaagg aacggctcta acttccaagg catccgtttc gtcatcgaaa
      121 attacatact tggcagagtt attttcatca cgcggctgac caacactgcc gatattgatg
      181 atatatttcg actggcgact gagttgacga caaccttttt tcaaaatgga agtatgaata
      241 tttttattct cagcatctga ttcatataat ccgagttgat gggtatggcc gacgaaagta
      301 atccattcag gtgttgaggc aaaagctttg cgtaattcgt gttctccgta atagttaata
      361 taatctttat atgagtccgg gggagtgccg tgtacgaaac gcagattgtc tactgttaaa
      421 aaggtcggta gagtttttat atattccaag gtctgctctg agatcaactg acgggttatt
      481 ataattgatt cgaatgcatc aacggaaaag taattgagca gttcatcatc cgtgagagca
      541 acttcatgat taccaacaat ccccgcgatt tgtagttcgc gtaaaagctg ggtaacatct
      601 tccggttccg gaccataacc gacgaaatcg cccaggtcaa cgatgcggtc aatttgttgc
      661 tgatcaatat cagccaggac ggcttctaga gccgccaggt tgccatgaat atcggctatg
      721 attgcaaatc gcattgctca aactttaaaa ttgaaaaatt attattagga aatgaacctc
      781 ttctgataat cgcttcgctg gtaaaaacta cattcatatt aaaccaagcg caaattacat
      841 aacaataata ttcttttttt gtcaaaaagg caagaacata atcatcacct acctcaaata
      901 tttttaaatt ttaatcaatc caaggtaaat gatttttgaa tcaataattt atacaatact
      961 ttaactatgg tctattttga attctatggt cgttcgattg acatccgccg cgccacagtc
     1021 aaggtattgc agagattcat ctaatccaaa aacatattac ctgatgggga cgaagcaatc
     1081 tatttttctc aattgctttc ggcagtattt cgcttgggtc ttcatcaatc tcaacttacc
     1141 tggtggccgt ttagggtaaa atttagtgag tgcttagatt caatcagtta aaaactatat
     1201 ctgttcttct taacctgaac cggtggattg attataggac gataggtcaa tgtctataga
     1261 cttcttaacc gcttaaaaaa ttttcttccg gaagtgacag tacggctttt ttccggtttt
     1321 ggtgtaataa tcctggtgat aatcttcggc tggataaaat gctccggctt tttctatttt
     1381 tgtagcaacg gcgaatccct tccgttttaa taacgcaatc agatcttccg cgactttctt
     1441 ttgaaactca tcgaggtaaa aaatatagga aagatactgt tcgccccgat ccggtccctg
     1501 cccgtcagtt tgagtaaagt cgtgaatttc aaaatagagt ttggtcaatt cttcataact
     1561 tgtaactgtc ggatcgtagg tgacctcaat agcttcttta tgaccggttt tgccggaaca
     1621 aacttgttta taagtcggat tggtggtatg tccgccggta tagccgacgc gggttgccag
     1681 tacgcccggc gcctgcttga aataatattc tacaccccag aagcatcccg cggcgtaaat
     1741 tgccaatgcg gtttccgagg tatcttttgc cggaataaaa ttcagggaaa tcgaattgac
     1801 gcaatggcgg ttgttcttag ctgtaaaatt ttcaccggtg aaaaaatgtc cgagatgagc
     1861 accgcaattg gcacagacta tttcagtgcg ccgaccgtct tggtcgagaa cccgcataac
     1921 agcgccggta atttcatcgt caaaacttgg ccagccgcaa ccggcatcga atttatctct
     1981 cgaacggtag agcggcgcat cacagcgttt acaggtgtat atgccatctt cataaaagtc
     2041 ataatattta ccggaaaatg gtcgctcggt gtctttatga atgatgactt tttcttcatc
     2101 aggcgtgagt ttatggtata tcattttttc ttctccaata ttttggttta tcaagattag
     2161 gaaaatgctg agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttacctac
     2281 gaatcgcatt ttccccgaag tacaatctat aacctcaatt agttcaaaga gtttattagt
     2341 aagtgataaa attggttgtt attcgcccga aaactgtcta tttttgaact tgtgaagtat
     2401 cattattgtt atcattgcgg tacgccaaca acttcgctcg aaatggaagg ctaccgacgc
     2461 gcattttgcc ctcaatgtca gctcattcta tatgaaaatc ctctacccac tgtcgttata
     2521 attgcttata acgaacaaaa tgaaattgtt ttaattcgtc gtgcagtcga accaggacgg
     2581 ggcggctgga gcctcccagg tggattcatc gaaataggtg aaaccgcggc gcaagccgct
     2641 gttcgtgaac tttgcgaaga aaccggttta agcgctacgg agttaaaatt ggttgatgtt
     2701 ggcactcact tgaatggatt ctatggtgat attttaatga ttggttatgc tacaccggtc
     2761 caagaagatt taattcgtcc tggcagtgat gcggcggaag ccgcctggtt taaaatagac
     2821 gatcatccat ctctggtttt tccctttcat gaaaaattaa ttgaaaattg gcgaatgata
     2881 aaataacctt ggttacgaaa aagcaaaaag tattagtagc tctgagtggc ggcgttgaca
     2941 gcgcagtggc ggcttgggtg ttatgtcaag aaggctatga accggtagga atcacttttc
     3001 ggatgtggcg aatgaaagaa gatattgccc aggcaaaagc cattgatcgc gctcggcaag
     3061 tttgtgaaat attgaaaatc ccgcatataa ttttcgattt aaatgcagaa tttgaagagc
     3121 aagtagttaa accattcgtc aatgaatatt ttcaaggatt gactccaaat ccatgcgtga
     3181 attgcaatcg cgttattaag tggcataatc tgcagaaaat ggccgaccag tatggaatcg
     3241 aaaaaatcgc caccggtcat tatgcccgtc ttaaatataa ttccgcaaac aaacgctacc
     3301 agattttgaa aggagtggat tcaaccaagg atcaatcgta tgctctctgg caaattccgc
     3361 aaaccgcact gaaacgcacc ctttttccgc tgggcgaaat gactaaaaat gcagtgaaaa
     3421 caattgctgt tgatcataat cttatcgtcg gtaaaatcac cgaaagccag aatatttgtt
     3481 ttatccccga aggcgactat cgtagtttct tgaaggaata cgcgcctcag aaagtgcaaa
     3541 ccattggacg gggcgaattg gtggacgaac gcggccgagt tttaggctgg cataatggtt
     3601 tttacaattt tacaattggt cagcgcaaag gcttccgcgt cggtttcaaa gagcggcgtt
     3661 atgtaaaagc cattaatgcc ccgaaaaatc gggttgtgat tgcgcctgat aaatcgcttt
     3721 tctcttcgaa aatgataata aaaaatgtca actgggtcgg aatatccgcc gtagctgaat
     3781 tcgacggcat aattaaaata cgttataatc atccgggcgc tatgggtcat ggaaaaagga
     3841 ttaatgataa cgaatatgaa attactttta ttgaaccgca aagagcgatt tgtcccggcc
     3901 aatcggctgt agtttatcag gatgattgcc ttctaatggg cggaatcatc caataaattc
     3961 gatgatcaat gtccgacata ttgggaaaaa tatttaaatt ttaagcaaga aatggtgagg
     4021 aaatgcaaga taaagtacga gtctcaacta ttctcgaaat gaaacaaaaa ggcgtgaaaa
     4081 tcacatcctt aacggcttat gattttacca tggcgcactt aatggacgaa tcgggtgttg
     4141 atattatcct ggtcggcgat tcctgcggga atgtctgcgc cggatacgaa actactttac
     4201 cggtgacaat ggatgaaatg gtctatcacg tgcaatgtgt gcggcgcgga gtcaaacggg
     4261 ctttgctggt agccgatatg ccgtttatgt cctatcagga aagtgtcgaa caggcactcc
     4321 ataacgctgg gcgctttatg aaagaatgcg gcgcagaagc agtaaaactc gaaggtggtg
     4381 aaccgatttg tcctgttgtt gaaaaaatgg tcgaaatcgg aattccggta atgggacatc
     4441 ttggttttac tccgcaaagt gtccataaat tcggtggatt tggtgtgcga ggtaaagaaa
     4501 ctgaagaagc caaatatatt gaaaaatcag cgtttgcctt acaggacgcc ggggcatttg
     4561 ccattgtgct ggagaaaatt ccagccaaac tggcggcaga tgttaccgct cagctaagaa
     4621 ttcccacgat tggtattggc gccggaaaag aatgcgatgg gcaggtcctg gtgtcttatg
     4681 atatgctcgg attattcgat caggttaaat ataaattcgt gcgccgatac gctaatctgg
     4741 cggatgaaat tcgccgagcg acgacagcct ttggtaacga tgtacgcaac ggcaattttc
     4801 cggacgaatc tgaaagttat tgaagcagct caaaattaaa taaaatatgc agatattgac
     4861 taaaatcgaa gatattcgtc aggaattatt agtaattcga accagcgcga agaaaatcgc
     4921 gcttgtacca acgatgggct acttacatga ggggcatctc tcactagtag atgtcgctaa
     4981 agccaatgcc gatgtggtcg ttatgagcat tttcgttaat ccgactcaat ttgccccgaa
     5041 cgaagacctg atgcgttatc cgcgcgatat cgagcgtgat gagcgtttag cccgggaacg
     5101 cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaacaag ctccgattat
     5221 tttcaaagct ttgcaagccg cccgagaaaa tgtcaagaat ggtctgaccg acgccaacca
     5281 ggtccgtgac caaattacta aaatgattca gacttcgcca ctggcgcgga ttgattatgt
     5341 cgaaattatt gatgatcaaa cgctgacgcc ggttgaaacg gtaaagccgg gaactttcgc
     5401 cgcggtagcc gtgtattatg gcaagacgcg tttgatagat aatatttatt tgaaagaaag
     5461 ataattttaa agaagtgtga gtttggtctt gacaaactcc cgtaaaagat ttaaaatgta
     5521 gtatgaaaaa tgtcaatggc ggtgttaaga tgaaaagaaa ttttttaaca gtgacaatgt
     5581 tggcgatttt tctcggaacg agcggatttg ctcagctgaa gactcagatt caaaggtcac
     5641 ctacaatcgg cgaagcaatt catttgcccg gtatttcatc cgcaatatcc aattatagcc
     5701 tatttgatcc cagccggttt aaaatgcaac agtcttattc tttatcctat tcgacctttg
     5761 gtaatcaatc gatgtcggta ggagtttacc aaaatgcgat gtcctttttg ctctcgaaca
     5821 aatttttact aaacacccgc tttggatttt atcataatcc gctgcaatta gggacgttta
     5881 acgctacctc aaataatatg ctcgataatt taatatttgg agctgaccta atatatcgtc
     5941 ctaaggaaaa cgccaccttt atgattcgtt ttgaccgcag accatattac tatccgtatg
     6001 agtattacaa tccttacttg ggatattaag agtggcacgt agtcgtaatc gtcgcaaacg
     6061 ccctacctct cgtaaaaaca aacgccgggc agttgacctc caaaaatggt ttctcaatct
     6121 ggcaattttt tctatttcgg tagttattgt tggttttatc ttctcgatgg gaaaacgctt
     6181 taacaatcca aatgaaaaat taactatgtc gcaggtgaat gtaattccac ccgatgaaag
     6241 accggtaccg tacattgtta ttgaagtctt aaatggaact gatgtacccg gtttagctca
     6301 aaaattcacc aactttctac ggcaaaatgg ctatgatgtt atttcaaccg gtaacgctga
     6361 ttattctggt tacgaaaaaa ctatgctgat aacccgtacg gcggatgtta ccaaattgcg
     6421 ggaagtgaac ggaactttac tgcttgattc cgatcaattg tttcagaaaa tagactcaac
     6481 cctccaggtt gacctgactt tgattgtcgg caaagattac caacgcttgc cagtttattc
     6541 taaaataata gctatcggag agacgtttta atttttgaaa acctctcaga atactaaaga
     6601 taaaaatcaa tcctaccctt tagcccggca aattgcggac atcgcagttg ataagaaagc
     6661 gcgtgatgtt gtcataatag atttgcgacc gttgacttcg ataacggatt atttcgtgat
     6721 ctgtaccggc gcggttggtc agcacgtcaa agccatcgtt gatgaaattg atgatcgtct
     6781 gcgaccggct gaaaagccct ggcacatcga aggttaccag cacctcagct gggttttgat
     6841 ggactatgtc aatgtcgtgg tgcacgtttt caatcctgag gcgcgtgcgt attataatct
     6901 tgaaagactc tgggcagatg ccgccacgga aatgattagt gatgattctg ccgattgatc
     6961 gtcgagtata atcggcaatg tttttaaatt tttattgaaa aaatattgat gagtctgacg
     7021 gctgaaatta ttcgtctggc tgagaattcc ggattggcgc gtgtgggaat ttctccgcct
     7081 tttttagact ataaatatat cacttttttc cagcaatgga ttgatgcggg atttttaggc
     7141 acgatgagct ggatgcggaa atattacgat tttcagaatt atcagaaaac gtacttcagc
     7201 tgggcaaagt cggttctggt ggtagcggat aattattttc aaaatgtaga gtggccaacc
     7261 gggattccca aaattgcgcg ttatgcttgg ggcaaagatt atcataaagt tgttaaacaa
     7321 aaattggcga aagttagtcg cgaaattaat ttattggagc cggcgactgt atcccggatt
     7381 tacgttgatt cgggtccggt aatgcaaaaa atcttcgcct ggcaagccgg tttaggctgg
     7441 gtcggaaaaa acaatttgct gatcgtcccg gatctcggtt cgtattgttt tatcggaatg
     7501 atttttctga atcttgagct ccattttaac gaaccgcagt cgaatctttg tggcgattgt
     7561 caactttgtc gggatgcttg ccccaatcaa gccctggtgg atgagcattt attggatgct
     7621 cggctttgca cggcatatct gactattgag aaagacggcg aattttcaat ggctgagaag
     7681 gagaatttac gcggctggat ttatggctgt gatacttgcc agaaaatttg tccgtataat
     7741 caaaaatgga agcggccagg agatgctcga tattctgaat attactcgga gttcagcaag
     7801 tcactaactg attggctgaa agttacagaa gatgaattcg gtgaaatctt ccgggatagc
     7861 ccgatccgtc gcattggctt tgctaattgg cgacgcaatc tactgacgct ccgcaagtaa
     7921 attaaattta tacggtaatg acgtttgcac aggaatagac gggcgcttaa atttagaccg
     7981 atttttttaa aggaatgaaa tgatagaaca tatccgcggt ttgctggaaa aagctcttca
     8041 gcaaaacgac cttgattacc gcgaaccgat tcagattgag cgaccaaaac aggaaacttt
     8101 cggacattat accacaaatc tggctttagc ggtggctaaa atccagcgtc aatctccgct
     8161 tgaaattgct gaaaaattag ttgcaacgct tccccaatcc gagtatatag aacgagtgga
     8221 atacagggcg cccggattta taaatttttt ccttagtccg cgtgtgcttc agcaaacagt
     8281 tgcccaaatc attttagccg ctgaaaatta tggcaaactc gatattggga aaaatcagcg
     8341 cgtacaagtc gaatttgtca gcgctaatcc gactggtcca ctgactgtcg gtcatggtcg
     8401 tcaggcggtt ttaggcgata ccgtagcgcg tattttggaa tgggttggtt accgagttga
     8461 tcgcgagtat tattataacg acgccgggcg gcaaatgcgt atgctcgggc attcggttta
     8521 ttggcgttat cgccaattgt tgggttggaa gggcgaattt cccgaggacc attatcaagg
     8581 cgaatatatc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtctttatgg
     8701 aggcacagac gaccgcgttc tctggaaaag cgtcgtagat gaagctacct atcgtttgcc
     8761 ggatatcgcc tatcacgtca ctaaatttga acgcggctac gatttgataa ttgacatttt
     8821 cggagccgat catcacgcta cttatccgga tgttctggct ggtttgcgcg ccttaggcta
     8881 tgatacttcg cgagttaaag tcctgattca tcagtttgtg acgttaatta aaggtggtga
     8941 aaaagtaaag atgtcaaccc gcaaggcgaa ctatgtcacg cttgatgaat tgattgacga
     9001 tgttgggcgg gatgtagcgc gttatttttt cctaatgcgc ggtatgaaga gccatttgaa
     9061 ttttgatctc gatttggcaa aaaccgagag tgaagaaaat ccggtttttt atttgcaata
     9121 tgcccatgcc cgaatttgca gcattttgcg gaatgcggct acgaaaaatc ttgccgtagg
     9181 aaatgacttc gatatgactt tattgaagga agaagagact ttaagcttgc tcaatgcgct
     9241 cagcgaattc aaagaagtta ttgaaatttg tgttagcact ctcgagcccc aacatctaac
     9301 aacttattta caaaaagttg ccactgcttt ccataaattt tatacagttc atcgcgtcat
     9361 ttcaaaagat tgggaattgt cgcaggcgcg cctggcgctg gtcaaagccg tccaaatcgt
     9421 tatggctaat gggctggcgt tactgggaat ttccgcaccg gaacgtatgt agtaaaattt
     9481 tcatcggcga acaaaaacta agccaaacag aattccagtg cttgatttaa gcaggtaaaa
     9541 tctttaaatt gctcggcgaa attagaagta tgggctgtaa aaatgcgagt tgatctaaaa
     9601 acggcggaga aaatagctga tttggcgcgc ttgaccttga gcgaggcgga actcgagcga
     9661 tatgccgctc agatggatca gattttagag tacattgagc agttgcaaaa cctggataca
     9721 agtaatgtca agccgctatc tcacgtccaa gaaataacta acgtttttcg aaatgatgaa
     9781 gtcaaaccgt ctctacccaa agaggcggtt tttgctaatg ccccacaaga aaaagccgga
     9841 tacttcgttg ttcctaaagt catcaaggac attaatccat gaaagtgctg ggggtcattc
     9901 ccgcacgttt tgcggctacc cgtctgcccg gcaagccgct ggttagcctc cacggaaaaa
     9961 cgcttatcca gagagtatat gaaaatgccc gtaattctca attgattaat caattaatcg
    10021 tagccaccga cgaccagcgg atttttgaac acgttaaatc attcggcggg gaatgccgaa
    10081 tgactccggt tgaattggct tccggtaccg accgttgtgc tcttgtggcg cgggataacg
    10141 aagctgatat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tcacgtggtt
    10261 ttaaggtaac cattcagaaa ttcgatccgt atattaacat tgatcccgga acgatgagcc
    10321 cattccagca tggcgaggtt tttgtcctgg aagatggttc cgagactgac ctcgatctgg
    10381 gccattatga gcgtttcatt gatgaaaata tgaacacgcg caatagcaat acttcaggaa
    10441 aaatttacta tgaagtgatt aatcgcgaac ggcgtggtga ttatctggga gcgacggttc
    10501 aggttattcc gcatatcacg gatgaaatta aacgccggat aacggctgtt aatgtcgatt
    10561 ctgcttatga cgttgttatt tgcgaaatag gtggcacggt cggcgatatt gaaagtcagc
    10621 cgtttctgga agctattcgc cagtttcgtt tcgaatcagg gcgtggcaat tgcatcaatg
    10681 ttcacctaac tctcgtgcca tacattgaaa ccagtggaga aattaaaacc aaaccgacac
    10741 agcatagtgt tatgcgtctg cgcgagattg gtattcagcc tgatatcctg ctatgtcgct
    10801 cgaagtttca tctcgatgaa agcattcata aaaagatagc ccttttctgt aatgttatgc
    10861 cggaagtggt cattgatacc attgatgccg aaacaattta tgagattcca ctgatttttg
    10921 atcaacagaa gttgggtgat cagattaccg atctgttaga tttaccgcgc cggaaaccag
    10981 acctcaaaga tttggagaag tttgttcagg cgataaaaaa tccaaaggac gaggtcaaca
    11041 ttgcgatttg tgggaaatat acccagttgc acgatgccta taaatcaatt atcgaatcat
    11101 tcaaccatgc cggtgcggag aataatacgc gcgttaatat agtctggatt gaatcggaag
    11161 acttgaccaa tgactgtgaa actgtcaata aactaaaaaa tgttgatggc attctggttc
    11221 cgggtggttt tggcgaacgc ggtgtcaaag gcaagattaa tgcgattaag ttagctcgcg
    11281 aaaatggcat tccgtttctc ggtatttgtc ttgggctaca atgtgctgta gttgaatttg
    11341 ctcgtaatgt atgcggctgg aaggacgccg atagtacgga attcaacgaa aatacccagc
    11401 atccggtcat tgaccttatg gaggagcaga agcgcgtttt caataaaggc ggtacaatgc
    11461 gtctcggggc ttataccgcc gaagtcattc ccggtacttt agcctatgaa atttataaaa
    11521 ataagcaaat ttccgagcgt catcgtcacc gctatgaagt caataatact tatttggctg
    11581 aaatggtcaa agccggtttg gtcgtcggtg cggtcaataa agaactgaat ctggttgaga
    11641 tgattgagtt gaaaaatcat ccctggtttg ttgcttgtca atttcatccg gaaatgaaat
    11701 cacggattaa taaagcccac ccgatttttc gtgaatttgt gcgcgcggcg cggaaatatc
    11761 ataaccaaaa agcggaaaat gccagaagtc aaagttaaaa atatcgccat cggcaaccat
    11821 caaccattgg cttttattct tggaccctgt gtaatcgaaa gccgcgatca cgctttgaga
    11881 atggctgaaa agctaaaagc actctcggaa gaacttgatt ttccgctgat ctataaatct
    11941 tctttcgata aagctaatcg cagttcgatt gagagctttc gcggacctgg attggaagct
    12001 ggtctggcga tcttagcgga agtaaaacag caatttggct taccgattct gactgatatt
    12061 catgaaccgt ttcaggctga aatcgtcgct aaagtcgtgg atgtattgca aattccggct
    12121 tttttgtgcc ggcagactga ccttatactg gcggcgggtc gaaccggaaa agtggttaat
    12181 gtgaaaaaag gacagttctt
//
LOCUS       DAOOMU010000069        11857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_5997_length_11857_cov_8.585748, whole genome shotgun sequence.
ACCESSION   DAOOMU010000069 DAOOMU010000000
VERSION     DAOOMU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5997_length_11857_cov_8.585748"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2315)
                     /locus_tag="PLI55_07370"
     CDS             complement(<1..2315)
                     /locus_tag="PLI55_07370"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HPB00694.1"
                     /translation="MKRFSELFKLALCFSFCFLGLQAQSQMIANISEDVQTDFGIYQP
                     YTANFTPDVPLFSVEPDFSNVENFSDFYGFSAVDSALLLQNHFTVRRSQFKQLYDIYN
                     DCTWDGTPLFVTTDAVLHIYHVLYDKILAEIEIQKFVPALELLTKTLIDSTQSQYDTA
                     TEPEIKETLRRNLAFLCVSQKLLKGSDFTVPEPVSALVDSELTLIANHDGFYTPPVLG
                     PFNLLDYSQFIPRGHYTTNDTLTAYFKAMMWQGWTIFTMEPAKFDNLARRHTLQALLL
                     TQMLFNLDANGNSLLDLWKMIYEPTVFFVGKTDDPNILHYKTIAAQVYGSDFLSLSAD
                     SLANSTLLENFMTEAQKLPEPKIPNWIYGSFTTYKGFRLMGQRFIPDSYMFAHLVLPE
                     VGGRAFPKGLDIMAILGSDRAYDLLDSLYNETAYPNYPEKITEFKTEFAAISPAEWAQ
                     NLYWNWLYCLMPLLYEKGEGYPFFMQTIAWMDKELLTALASWAELRHDTILYAKQSMS
                     PCGIAPGPPKSYVEPNPHLYARLASLTRYTIDGLQARDLLTEKFRDKLALFESLLLFL
                     QDVSIKELENTPLTDEEYANIYCFGKVMQKLVSEGEDPWHYDSDDMAVVADVHTDSDS
                     DQCLEEGVGYPLEINVIVNEGGTIRITRGAIFSYYEFIQPIANRLTDEAWREMLASET
                     PPTMPQWYDGLMDMNASQPSFMENSPDNLYHGEFQAIEKPDDSQKPKTFQVRQNFPNP
                     FNPLTTIEYELPQYSHVRLEIYNLLGQKITTL"
     gene            complement(2373..>4097)
                     /locus_tag="PLI55_07375"
     CDS             complement(2373..>4097)
                     /locus_tag="PLI55_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00695.1"
                     /translation="ATVPDPVTRQPSIPYLMEPIGFRTMQMAIFAPEVSGMVPDKFSD
                     LSWNPAYILREKQKTFYIDFNLENSVTSSLNPLSSGILTDSYYLAPSWYSQTSVQDIN
                     IAPLYNLALILPLNKRMSLGIVNRSVFDYGPYRNSIWWTSGGWFDKAIESNSNIRYAD
                     FEPQRLETDENQQTVIANQSEITLGYRLSTKTDLGLHLGHYIFHRDGYLLDSQWATHP
                     HSSYGILNDEDLEINGCHYDLGAGFLYHPDEKTTYGISVGAFFGSGQESEIAQDTSDS
                     FSETATNTAYYCLSEHTLNTDLTYKTSGVKPYLTLILEKKLNKNLVLRSSFSLTSKNQ
                     DVNTRYATDDTTFSDRTYNHWITDTTYAFRRYTANSAAEFNLNGSGEESINQWKFMTA
                     LIYSLENDWAFFGGVQISRKWSQVDFDERSNYYSNSFTEYSLYNASTYRNLDTYTKKY
                     SYSQETKAWNLSVPIGVKAYLAKGFSVLLGTDINLTLYKENSYSDLLYPNIVSKYWIN
                     DALANDDPETNRYERYDSNPPKTLTRTNAVRFGMVYEHSSGAKLFIRSDGDIMNTNGW
                     TLGFEMNW"
     assembly_gap    4098..4197
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4198..5294)
                     /locus_tag="PLI55_07380"
     CDS             complement(<4198..5294)
                     /locus_tag="PLI55_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPB00696.1"
                     /translation="MEKTTRNFLILIIFVAGLISCSDVTAPDSVEIPELPRSISKSEQ
                     TLIESSNAFGFDLFKILSNKANGNNLFVSPVSVSMALGMTANGAAGSTLEAMHSTLGF
                     ENLTEDEINQSYQSLINLLRSLDSKVKFRIANSIWFRNEFTVLPTFIDVSQTYFDAVV
                     RGLDFNQDAAVDTMNAWIEAQTDGFIKQLIEKPINTNTMMFLLNAIYFKGTWTYQFNE
                     EHTQEAPFYLNNDNTANCQMMSLSSDFDYAETDELQIIDLPYNNGFFAMTVLLPQSSD
                     DLNSLISGMTDEKWNNLIAGLSTTTVSLYLPKFKLEWFQVLNDQLSELGMGIAFDNRA
                     DFSRINDQYGSELYISEVRHKSFIEVDEEGT"
     gene            complement(5310..6548)
                     /locus_tag="PLI55_07385"
     CDS             complement(5310..6548)
                     /locus_tag="PLI55_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HPB00697.1"
                     /translation="MANYKIMILTLLSLLTLNRCPEKITGGDKPEPIRELSAIEKKVT
                     EADNNFGLKLFRQIISSETDKNIFISPLSISMALGMAYNGADGETRAEMHSILEFGDL
                     TAAEINQSYRNLIELLSSADPDVTFEIANSIWYRAGFSILSEFLQLNTTYFDAVVQAL
                     DFDRSDATDIINAWVNAKTHEKIPTIINPPIDPLTVMFLINAIYFKGTWTYEFDPEKT
                     QDAPFYLKDGSQTTCEMMRHKATHGYYANDQIQIADLTYGEIGFSMTIILPNAVVDVD
                     SIAAMLTTENWTEWTNSLTEAEINIYLPKFKTEYEIGLIPTLQALGMIQAFSSSEADF
                     DKINPNYDLYISDVKHKTFVDVNEEGTEAAAVTAITVGCTSAGPKEIYFIVNRPFIFV
                     LREKSTSTILFIGKIMNPVS"
     gene            complement(6704..7756)
                     /locus_tag="PLI55_07390"
     CDS             complement(6704..7756)
                     /locus_tag="PLI55_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00698.1"
                     /translation="MLALYFRNLTLILFIIVLLLSGLLQADQKLSGYCKTEAGMETNI
                     LESDSTSQNSFTTPALANLHFYLNKRRLNFNVNATGGLIAYDYRPENKATFNISAQAN
                     YTVSRKFFLASTVNSFQKWWLEQDYAYYNTDWLIGGGLNLSHWNPSLSIILSQNLFRH
                     SSSLDNHRIGGLVEISSSLNPSLTFSAQSGYSQIDYLHRPLFSVENSVLDTTARQKDL
                     LFFGQIGLEHRRQALSGVRLKLITVVSNNDFSEYYGIGLNYYLSGKIGRGYFQVIADL
                     LLKKYRVDLSQYLLYYNPDPEQNIQNQLLVGWELPLWKNLALTGRAAIMRNETHYSGI
                     YYNKWFISGGLIYRKN"
     gene            complement(7768..8742)
                     /locus_tag="PLI55_07395"
     CDS             complement(7768..8742)
                     /locus_tag="PLI55_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00699.1"
                     /translation="MNNNFTSRIAICGTLILILLLAKNIQSAPIDSLAYYQTQYNQQL
                     TKLRTLEKDYDKLLNDINAIDAQIIKINNASNPNWNDKRKLSRLTARKAEINAKMIPI
                     YTELTNQRTILNITFNRYYDFLSKSIDSTVNQYNTVGDSRQRQSLSRTLFELIDQRGQ
                     LLASQRQFSDQNEFPLPEKEDLVALISKYDFNEAIRRDISHILDAKIRQIDLMIAAAN
                     TEKELRQRLNQLNLEMNALSGESYPYQGGNFSDYENWDHATMSGKSAYRNRTDKAALP
                     ATTQSVTAADYLPLFQNIPTNDLSLYISQLDSLRRDYQKQLQEINGNK"
     gene            complement(8735..10111)
                     /locus_tag="PLI55_07400"
     CDS             complement(8735..10111)
                     /locus_tag="PLI55_07400"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPB00700.1"
                     /translation="MNSPKSNYTILIVEDNQSMRDGLLASLQKEGYSVAGVADGQEAW
                     SLLQNQRFDLIITDIKMPGLDGLTLMKKVKNLAPMTNIILITAFPTVDIAVDAMKCGA
                     QDFITKPFALAELRQKVEALYRRKFVQLKPSLSEPEFPDLIGNSPAIRQIRQLIAKIA
                     PGDSPILITGESGVGKELVARLIHQASRQKNGPFLAVNCGALTETLLESELFGHEKGA
                     FTGAHKTHIGKFEQSNSGTLFLDEIGDMSTALQVKLLRVLQTKQFQRVGGEKFIASDF
                     RLICATNKNLEKKIKDGEFRSDLYYRINVIPLTIPPLRERPSDIPLLVEHLLRLKADR
                     LGRKIPRIEPSVMARLISYSWPGNVRELENFIERALVFIEEDIFSEKLFNFGDLPENT
                     SLLNQTDTPAARDLTAQISQIEREMIIKALRDQHGVKQRAAEQLRIKPSTLYYKLDKY
                     GIKPEEYE"
     gene            complement(10115..11560)
                     /locus_tag="PLI55_07405"
     CDS             complement(10115..11560)
                     /locus_tag="PLI55_07405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB00701.1"
                     /translation="MIGIKNNDIQSDNPQSGRGFFPPIKLSWKLVLFTVILILFVTAA
                     ITYPIYWQTRQALENQLTNHLEQNIQLVREQIDTSLVNLLADYPELNSVRDSLETKLN
                     WQAGKFSAQAIYLIDASGRLLVIAGNEESALQSATLHAVEIASARNDQVTSSPLFADR
                     TGQYYKSVFTKITENNGQPVVLGLDAGADFLEITGKLRNQILSLTAIILIISIGLVLL
                     LARTMTKPLENLTKYAEAIGQGRSDLPVLNHRRDEIGFLSRTIQTMYGKISQREKENK
                     QFLASVAHEIRNPLGGMKINAELLLEDTDNLTNATKYATAIAREVDRLALIVDSFLAY
                     ARPIETNLIICDLNELLQQVISELQRDFPSAKITVSGQARVKVNPGKIRLCLLNLIRN
                     GLEAVPEAPAIKINITNKPGQTEIRFFNAGMPIAPEIQSQIFEAFFSTKPDGIGLGLA
                     ITKSLVEQHGGQIYLDHSNTSGTEFVLILPE"
BASE COUNT         3139 a         2461 c         2771 g         3386 t
ORIGIN      
        1 agagtcgtga ttttttgtcc gagcaaattg taaatttcga gcctgacgtg ggaatactgt
       61 ggcaattcat attcaatagt cgttagcgga ttgaaggggt tgggaaaatt ctgcctgact
      121 tgaaaagttt tgggtttttg agaatcatca ggtttctcaa tggcctgaaa ttcgccatga
      181 tagagattat ccggtgaatt ttccataaaa gacggctgac tggcattcat gtccatcaaa
      241 ccatcatacc attgcggcat agtcggcggc gtttcactcg ctaacatctc acgccaggct
      301 tcgtcagtca gtcgattagc gattggctga ataaactcgt agtaggagaa aatcgccccg
      361 cgtgtgatgc gaatggtccc gccctcgttc acgatgacat tgatttccag cggatagccg
      421 acgccttctt ccaggcactg atcggagtcg gaatcagtgt gcacgtcggc gacaaccgcc
      481 atgtcatcgc tgtcatagtg ccagggatcc tcaccttcgg agaccaattt ttgcatgact
      541 ttgccaaaac aataaatatt ggcgtattcc tcatctgtta gtggtgtatt ctccagttct
      601 ttgatcgaaa catcctgtaa gaaaagcagg agggattcaa aaagagcaag tttatcccga
      661 aatttttcag ttaacaagtc ccgggcctgc agtccgtcta ttgtatagcg cgtgagactt
      721 gccagacggg cgtaaagatg cggattgggt tctacataac tcttcggcgg accgggagct
      781 ataccacatg gcgacatact ttgtttggca taaaggattg tatcatgcct tagttctgcc
      841 caggaagcca gagccgtcag gagttctttg tccatccagg cgatagtttg catgaaaaac
      901 gggtagcctt cgcctttttc atatagtaat ggcatcaggc aatagagcca attccaatac
      961 agattctgcg cccattcggc tggtgaaata gccgcaaatt cggtcttgaa ttcggtaatt
     1021 ttttcaggat aattcggata ggcagtttcg ttataaagcg aatcgagcag atcgtaagcc
     1081 cggtccgaac cgagaatcgc catgatgtcc aagcctttgg ggaaagctcg acctccaact
     1141 tctggtagta caagatgagc aaacatatat gaatccggaa tgaaccgctg acccatcagc
     1201 ctgaaccctt tgtaggttgt aaaagagccg taaatccagt ttggtatttt cggttccggc
     1261 aatttttgag cttcagtcat gaaattttca agcagagtcg aattagccag actgtcagcg
     1321 gaaagagaaa gaaaatcaga gccataaacc tgtgcggcaa tggttttata atgtaaaata
     1381 ttgggatcgt cggtttttcc tacgaaaaat acggtcggct cgtagatcat tttccacaaa
     1441 tccaataatg aatttccatt cgcgtccaga ttaaagagca tttgagtaag caacagagcc
     1501 tggagggtgt gtctcctcgc caggttgtcg aattttgccg gctccatggt gaaaattgtc
     1561 cagccctgcc acatcatagc tttgaagtaa gccgtcagcg tgtcattcgt ggtataatgt
     1621 ccgcggggaa tgaattgact ataatccaat aaattgaaag gtcccaagac gggcggagtg
     1681 taaaatccgt catgattggc gatcaaggtc aattccgaat ccacaagcgc ggaaaccggt
     1741 tctggaactg tgaaatcact gcctttcaat aatttctgtg acacgcacag aaacgccaga
     1801 ttgcgccgta acgtctcttt aatttccggt tcggttgctg tatcatattg cgattgggtg
     1861 ctgtcaatca gggttttggt caaaagttcc agagccggta cgaatttttg aatttcgatt
     1921 tcagccagta ttttatcata aagaacatga tagatatgca gaaccgcgtc ggtagtgacg
     1981 aaaagcggcg tgccatccca ggtgcagtcg ttgtaaatat catataattg tttgaactga
     2041 cttctgcgga ctgtgaagtg gttttgaagt aacagggctg agtccaccgc gctgaatcca
     2101 tagaagtcag aaaaattttc aacattactg aaatcgggtt cgacgctaaa aagcggtacg
     2161 tcaggggtaa aattggcagt atatggctgg taaatcccaa aatccgtttg aacatcctcg
     2221 gaaatgttgg caatcatctg ggattgagcc tgcaatccga ggaaacaaaa agagaaacat
     2281 agggcaagtt tgaataattc gctaaaacgc ttcatttttc gcctcgtttt tatctgattg
     2341 aaatttatag gcaaatttat taataatcaa catcaccagt tcatctcgaa acccagcgtc
     2401 cagccgttgg tattcataat atcgccatcg gagcgaatga aaagctttgc gccggatgaa
     2461 tgttcgtaaa ccatgccaaa gcggacagca ttagtgcggg taagagtttt gggcggattg
     2521 gagtcatatc tctcgtagcg attggtttcc ggatcgtcat tggctaaggc gtcatttatc
     2581 caatattttg aaacaatatt cggataaagt agatcgctgt aactgttttc cttatacaga
     2641 gtaagattaa tatccgttcc gagtaagact gagaatcctt tagcaagata ggctttgact
     2701 ccaattggca cgcttagatt ccaggctttg gtttcctgcg agtaggaata ttttttggtg
     2761 taagtgtcta gattacggta agtgctggcg ttgtaaagcg aatactcggt gaaggaattg
     2821 gagtagtaat tcgatctttc gtcgaaatct acctgtgacc attttcgtga aatctgcact
     2881 ccaccgaaaa acgcccaatc attttcgaga ctgtaaatca gcgcagtcat aaatttccat
     2941 tgattgattg actcttcgcc ggaaccgttc agattgaatt ccgcggcgct attagccgta
     3001 taacgtcgga aagcgtaagt agtatcggta atccagtgat tgtaggttcg gtcgctaaat
     3061 gtggtatcgt cggtggcgta tcgagtgtta acgtcctgat ttttactggt caatgaaaat
     3121 gatgagcgca ggacgagatt tttatttaat ttcttttcca aaatcaaggt taggtatggt
     3181 tttactccgg aagttttata ggtcaaatcg gtattcaaag tgtgttcact aaggcaataa
     3241 tatgcggtat tagtagctgt ttccgagaac gagtccgaag tatcctgggc aatttcagat
     3301 tcctggccgg aaccgaaaaa tgctccgacg ctaatcccat aggtcgtttt ttcatcggga
     3361 tggtacaaaa aaccggcgcc taaatcataa tggcagccat taatttccag atcttcgtca
     3421 ttcagaattc cgtatgagga atgtggatga gttgcccatt gcgaatccag caagtaaccg
     3481 tcgcggtgaa aaatataatg gccaaggtgc aagccgagat cagttttagt cgaaagccgg
     3541 tagccgagag taatttcgga ttggttggca ataaccgtct gctggttttc gtcagtttcg
     3601 agacgttgcg gttcaaaatc ggcataccga atgttggaat ttgattcaat agccttatcg
     3661 aaccagccgc cgctcgtcca ccagatcgag tttcgatagg ggccatagtc gaagactgaa
     3721 cggttgacaa tacccaggct catgcgttta ttcaatggca gaatcaaagc tagattgtaa
     3781 agcggcgcga tattgatgtc ctgaacgctg gtctgactgt accacgatgg cgcaagataa
     3841 taactgtctg tcagaatccc ggacgacagc ggatttagcg aggacgtaac actgttttcc
     3901 agattgaagt caatgtaaaa agtcttttgt ttttcgcgca aaatataggc tggattccag
     3961 ctcagatcgc tgaacttgtc cggaaccatg ccactgactt ccggcgcgaa aatcgccatc
     4021 tgcatcgtgc ggaatccaat cggttccatt agataaggaa tcgacggctg gcgcgtgacc
     4081 gggtcgggaa ctgttgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg
     4201 gtgccttctt catcaacttc aataaaagat ttatgtctga cttcgctgat atataattca
     4261 gagccatatt ggtcgttgat acggctgaaa tcagcccgat tatcaaatgc gattcccata
     4321 ccaagttctg atagctgatc atttagcacc tggaaccatt cgagtttgaa tttcggcaaa
     4381 tacagactga cagttgtcgt ggataagcct gcgatcagat tattccattt ttcatcggtc
     4441 atgccactta tcaacgagtt taaatcatca ctggattgcg gcaggagtac cgtcatggca
     4501 aaaaatccat tgttataagg caggtcaata atctgcagtt cgtcggtctc ggcataatcg
     4561 aaatcactac tgagtgacat catctggcag ttcgccgtgt tgtcattatt tagataaaac
     4621 ggagcctcct gggtgtgttc ttcattgaat tgatatgtcc aggttccctt aaaatagatg
     4681 gcattcagca gaaacatcat cgtattggtg ttgattggtt tctcaatgag ctgcttgatg
     4741 aatccgtcgg tttgagcttc gatccaggca ttcatggtat caacggcggc atcctgattg
     4801 aaatcgagac cccgaacgac cgcgtcgaaa tacgtctggc taacgtcaat aaatgtgggt
     4861 aaaacagtga attcgttgcg aaaccaaatc gagtttgcaa tcctgaattt gactttcgaa
     4921 tcgagcgatc tcagtaaatt gatcagactt tgatacgatt ggttgatttc atcttcggtc
     4981 aaattttcga agccgagtgt cgaatgcata gcctccagag tagaacctgc cgcgccattc
     5041 gcggtcattc ctaaagccat cgaaaccgaa accggtgaca cgaacaggtt gttgccattg
     5101 gctttatttg atagaatttt aaaaaggtca aagccaaagg cattgctcga ttcaattaac
     5161 gtctgttcgg atttactgat gcttctcggt aactctggaa tttctacgga atccggagcc
     5221 gtgacgtccg aacagcttat taacccagcc acaaaaatta taagtattaa aaaattacga
     5281 gtggtttttt ccatctttaa ttctccttat tatgaaaccg gattcatgat tttcccgatg
     5341 aataggatcg tgctggtgct tttctcgcgc aaaacgaaaa tgaacggacg attaacgata
     5401 aaataaatct cttttggacc ggctgaagta catccgaccg ttatggcggt cactgccgcg
     5461 gcttcggtgc cctcttcgtt aacatcgaca aaggttttgt gtttaacatc gctaatatac
     5521 agatcgtaat ttgggttgat cttgtcaaaa tcagcctcag aagaactgaa agcctgaatc
     5581 attcccagag cttgcaaagt tggaatcaaa ccaatctcgt attcagtctt gaacttgggt
     5641 agataaatat tgatttcagc ttcggtcaga ctattagtcc attctgtcca gttttccgtg
     5701 gttagcattg ccgcaatcga atcgacatct actacggcat ttggcaggat aatagtcata
     5761 ctgaagccaa tttcaccgta agtcaggtcg gcgatctgaa tctggtcgtt ggcatagtaa
     5821 ccatgggtgg ctttgtggcg catcatctca caggtcgtct gactgccgtc ttttaggtag
     5881 aatggcgcgt cctgtgtttt ttccggatcg aattcataag tccaggtgcc tttgaaataa
     5941 atcgcgttaa tcaggaacat cactgtcaat ggatcaatcg gcggattaat gatagtcggg
     6001 attttttcat gagtcttggc atttacccag gcgttgatta tatcagtcgc atcactgcgg
     6061 tcaaagtcca gcgcctgaac gaccgcatcg aaataggtgg tatttaactg caaaaattcg
     6121 ctcagaatcg aaaaacctgc tcgataccag atggagttgg cgatttcgaa tgttacatca
     6181 gggtcagcac tcgagagaag ttctatcaga ttacgatagg actgattgat ctctgcggct
     6241 gtcaggtcgc cgaactctag gatggaatgc atttcagcgc gggtttcacc gtccgcaccg
     6301 ttataagcca tgccgagcgc catcgaaatg ctcaggggtg aaatgaagat atttttatcg
     6361 gtttcggagg aaataatttg ccggaataac ttcagaccga agttgttgtc ggcttctgtc
     6421 actttctttt caatggcgct caattcacgg atcggttctg gtttgtcacc gccggtaatt
     6481 ttctcaggac agcggttcag agtaagcagg gaaagcagtg tcagaatcat aattttgtag
     6541 tttgccatag aagcctcatc atttattttc gcaatactta aaaacgacaa tttatttatg
     6601 acaaatagca ttccaagacc ttccgacgga gaaaatctgt ccggtataga caaaccctca
     6661 gctttctgag actaacatag aaaatctgag atttttgaga tgcttagttt ttacggtaga
     6721 tcaagccgcc gctaataaac catttattgt aatatatacc actgtaatgc gtttcgttgc
     6781 gcattatagc ggctcgacca gtgagggcta aattcttcca tagcggcaat tcccacccga
     6841 cgagcagttg attctgtatg ttctgctccg gatcgggatt gtaataaagg aggtattggc
     6901 ttagatcaac ccgatatttc ttcagtaata aatctgcgat gacctgaaaa tagccgcgcc
     6961 caatttttcc ggataggtaa taattcagac cgataccgta atattccgag aaatcattat
     7021 tggagacaac agtgattaat ttcaggcgca caccgctgag agcctggcga cgatgttcca
     7081 gtccgatctg gccaaaaaat agtagatcct tctgccgggc tgtagtatcc aaaacggagt
     7141 tctcgactga aaatagcggc cgatggaggt aatcaatttg ggaatagccg ctttgagccg
     7201 aaaacgtcag gcttgggttg agtgagctag aaatttctac cagtccgccg atacggtgat
     7261 tatctaggct actgctatga cggaataaat tttgggacag aataatggat agcgacggat
     7321 tccaatgact gagattcaaa cctccgccga tgagccagtc ggtattataa taagcataat
     7381 cctgttccaa ccaccatttt tgaaaagaat tgacggtcga agccagaaaa aatttacggc
     7441 tgacggtata atttgcttga gctgatatat taaaagtggc tttattttcg ggacgataat
     7501 cataggctat taatccgccg gtcgcattga cattgaagtt caatcgtcgt ttgtttaggt
     7561 aaaaatggag attagccagg gcgggtgtcg taaaactgtt ctgggaggtg gagtctgatt
     7621 ccagaatatt ggtttccatc ccggcttcgg ttttacaata accacttaac ttctgatcgg
     7681 cctgaagtaa tccagataat aacagaacga tgataaatag gattagtgta agattgcgaa
     7741 aatagagcgc caacattcaa cggcaattta cttatttccg ttgatttcct gtaattgttt
     7801 ctggtaatct cggcggagtg agtcgagctg tgaaatatac aaagacagat cgttcgtcgg
     7861 tatgttctga aaaaggggta gatagtcggc ggcggtcaca gattgagtcg tagccggaag
     7921 agccgctttg tcggtccgat tacgataggc agattttccc gacattgtcg catgatccca
     7981 gttctcgtag tcgctaaagt tgccgccttg atatggataa gattcgccgc tcagggcatt
     8041 catttccaga ttcaattgat taagtctttg gcgtaattct ttctccgtgt tggcggcggc
     8101 gatcattaaa tcaatctgcc ggattttcgc gtcaagaata tggctaatat cccggcgaat
     8161 tgcctcatta aaatcgtatt tggaaattag tgccaccagg tcttcctttt ccggtaacgg
     8221 gaattcattc tgatcgctga actggcgctg gcttgccagt aattgtccgc gctggtcaat
     8281 taattcaaat aaggttctgc tcaaactttg gcgctggcgc gagtcaccga cggtattata
     8341 ttgattgaca gtcgagtcta tacttttcga aagaaaatca taatagcgat taaaggtaat
     8401 attcagaatt gtcctttgat tggttaattc ggtataaatc gggatcatct ttgcattgat
     8461 ttcagccttt cgggcagtta ggcgggaaag tttccttttg tcgttccaat ttgggttgct
     8521 ggcattgttg attttgataa tttgggcatc aatagcattg atgtcgttaa ggagtttgtc
     8581 ataatccttt tcgagagtgc gtagtttggt taattgctgg ttatattgcg tctgatagta
     8641 agccaggctg tctattggcg ctgattgaat atttttcgcc agtagtagga ttagaatcaa
     8701 cgtcccgcaa attgcgattc ttgaagtaaa attattattc atattcctcg ggcttaatgc
     8761 catacttgtc caatttgtaa tacaaagtgc tgggtttgat tcgcaattgt tcggcggcgc
     8821 gttgcttgac gccgtgctgg tcgcgcaggg ctttaatgat catttcgcgt tcgatttgac
     8881 tgatctgagc agtcaggtcg cgagccgctg gagtgtcagt ttggttcaat aaactagtat
     8941 tttccggcag gtcgccgaaa ttaaatagtt tttccgagaa aatgtcttcc tcgataaata
     9001 ctaaagcccg ttcgataaag ttttccaatt cccgcacatt acccggccat gaataagata
     9061 ttaagcgagc cataacgctg ggttcgattc gcggtatttt acgaccaagt cggtcggctt
     9121 tgagccgaag taaatgttca acgagtaacg gaatatctga ggggcgttcg cgcagaggtg
     9181 gaattgtcag aggaatgaca ttgatgcgat aataaaggtc tgaacgaaac tcgccgtctt
     9241 tgatcttttt ttcgaggttt ttattagtgg cgcaaattag tcggaagtcg ctggcgatga
     9301 atttctcacc accaacgcgc tgaaattgtt tggtttgcaa tacgcgcagg agtttgacct
     9361 gcaaggcggt ggacatatct ccgatttcat cgaggaacag ggtgccgctg ttactttgtt
     9421 caaattttcc gatatgagtt ttatgagcgc cggtgaaagc gcctttttcg tgaccaaaga
     9481 gttcgctttc caaaagtgtt tcagttaaag ctccgcagtt gacggcgaga aatggtccgt
     9541 ttttctggcg actggcttga tgaatgagcc gggcgaccaa ttctttccca acgccactct
     9601 cgccggtgat tagaatcgga ctatcgcccg gtgcaatttt cgcaattagc tggcggattt
     9661 gtcggatagc cggtgaattg ccgatcaaat ccgggaattc tggctcgctt aggctgggtt
     9721 taagttggac aaacttacgc cgatacagtg cttctacttt ttgtcgtaat tccgccagcg
     9781 caaatggttt cgtgataaaa tcctgcgcac cgcatttcat tgcatccact gcaatatcaa
     9841 ccgttggaaa agctgtgatc aggatgatgt tagtcatcgg cgccaggttt ttgacctttt
     9901 tcataagagt cagaccatcg aggccgggca ttttaatatc ggtaatgatt agatcgaatc
     9961 gctggttctg aagcagactc caggcttcct gtccgtcagc cacacctgcc acggaataac
    10021 cttccttctg aagtgaagcc agcagtccgt cgcgcataga ttggttgtct tcgacaatca
    10081 agatggtata attggatttg ggcgaattca tattttactc cggtaaaatc aagacaaatt
    10141 ccgtgccgct ggtattggaa tgatccagat agatctggcc gccgtgttgt tcgacgagag
    10201 atttggtgat ggctaatccc aaaccgatgc catccggttt cgtgctgaag aaagcctcaa
    10261 aaatctgaga ctggatttca ggtgcaatcg gcataccggc attgaaaaag cgaatttcag
    10321 tctgacccgg cttattcgta atattgattt ttatggcggg tgcttccgga acggcttcca
    10381 gcccattgcg aatcagattt aatagacaaa gccggatttt gccgggattg acttttaccc
    10441 gcgcttgccc gctgacggtg attttagctg acgggaaatc gcgttgcaat tcgctgataa
    10501 cttgttgtag caattcgttc aaatcacaga taatcagatt ggtttcgatt ggacgggcat
    10561 acgccagaaa agagtcaacg atcagtgcca gtcggtcaac ttcacgagcg atcgcagtgg
    10621 catatttagt tgcattagtc agattgtcgg tgtcttccag cagtaattca gcgttgattt
    10681 tcataccgcc caatgggttg cgaatttcat gggcgactga agctaagaac tgtttgtttt
    10741 ccttctcacg ctgacttatt ttgccataca ttgtttgtat agttcggctc aagaacccga
    10801 tctcatcccg tcggtggttc agaaccggaa gatcgctgcg accctgaccg attgcctccg
    10861 catatttagt tagattttcg agtggtttag tcatcgtacg cgccagaagt aaaaccagac
    10921 caatactaat tatcaatata atggcagtta agcttagaat ttggttgcgt agcttacctg
    10981 taatttccag gaaatcggca ccggcgtcca gtccaagcac aaccggctgg ccattgtttt
    11041 ccgtaatttt tgtaaaaaca gatttataat attgaccagt gcgatcggca aacaggggcg
    11101 aagaggtcac ttgatcgttt cgagcgctgg caatttcgac tgcgtgcagg gtggctgatt
    11161 gtaatgcgct ttcctcattt ccagcgatta ccagtaagcg accgctggcg tctatcaggt
    11221 aaatggcttg agctgaaaat ttgccagctt gccagttaag tttagtctcg agtgaatccc
    11281 gcacggaatt taactccggg taatcggcga gcagatttac aagggaagta tcaatttgct
    11341 cccggactaa ctggatattt tgttccagat gattggtcaa ctggttttcg agcgcctgcc
    11401 gcgtttgcca gtaaatcgga taagtgatag ccgcggtgac gaacaggatc aaaatgacgg
    11461 tgaaaagcac cagcttccac gagagtttaa tcggcgggaa aaatcctctg cccgattgtg
    11521 gattatcaga ttgaatgtca ttatttttta tccctatcat tagtcagagc taagttaatt
    11581 gatttatttc aggaccaaat ttcttcttat ttccaaatac cgctcaaact gagcgagact
    11641 tcgtgaatcg gttatgaaat acataaacat agctaaattt agaacattta gcgaattacc
    11701 aaatcggaaa tataaaaccg gcaagtaact cgccttaaaa atcaatttct gctcgtaagc
    11761 ggctggtctt ttcctatagc aaataaattt ttactaaaaa ctcttgactt tgggagtcta
    11821 attagcttta attctggaaa attcagaggg aaaaaat
//
LOCUS       DAOOMU010000070        11613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_6198_length_11613_cov_22.590327, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000070 DAOOMU010000000
VERSION     DAOOMU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11613
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6198_length_11613_cov_22.590327"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..>3241
                     /locus_tag="PLI55_07410"
     CDS             111..>3241
                     /locus_tag="PLI55_07410"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPB00702.1"
                     /translation="MKITRLGVIISLLIMLIGLVFFDSADAVEKYEPLRLIRADKLEQ
                     FYRADTPIKRLTGNVLFQKGGLQLACDLAYWFERDQRADFYRNVIATSDNKVLKADSL
                     VYFSENDSIVARGHPILYDSTLSITARRLIYLVKDEIAHAEGKVLLKDSSRSVRANLI
                     HYYSNEKKSVAIGQAVLYDVKRHLELASDSLIYFNKTGNIFALNRPMLTRFDTAGVES
                     FRIYADTIRLVEKEGNFQAIGNVRIQSQDFTAYSKTLTYIDSLEVATLTDNPRVLSDG
                     QELTGKVMRLHFENEKISALYIDEDARANAVGKAYLPAESDSLAHGDSIKTYDEITGK
                     FMEIYFRDDQADSIVVAVMATSLYNMLEDSIILGINEVSGDTIRLKFIDRKINTITVV
                     GGTQGRFIPHETNTDLDTTVIYSAERIDYFVDRKLTDLYLNARIQYADIELTAGKINI
                     NWNENLLYAYPLSPPPYDSTSVDLPVLNQRGREPFVGESMVYNLKTQRGRIVSGKTKE
                     QDGYYYGDKISKVDKKVFYVNDCVYTTCDIPDKPHYYFRSKQLKIIFKDKVIARPVIF
                     YIHDIPLLGLPFIIIPDQSGKRHSGWIMPSYGESRIGGGFIRGLGYFWAPNDYYDFRL
                     TGDFYDKQGVILDYRLRYAWRYKLNGTISGKYTNDFFSNYPEKQWTFNITHNQTIDPT
                     TRLTVNGKFVSNESLYKKYSYDLEDRLDQQLISNATLSKSWTGKPYSLSVNFNQTTNL
                     QANALSQIEPTDSNLTIAHISRSLPNISFTRSSKPLIPLKPHQDVSRSKWYNNIYFSA
                     SSYLKNNQNIFYQSIYNPATDSLEWQEQNIAKNAVTYNFSFTSSQKIFGFLTTNQTVN
                     LDEGWVFKYDMPLYDEDHRFQLENGKIKTETVEGFKARHTGSATISAQTKAYGLFPVR
                     IAGLQAVRHVVTPSVSMTFRPDFSREIFGWDPGYIIRGIDTTGTVRTFDPLTNTMLGA
                     LSSYETRTMSMSLSNLFQAKLGQGEKTRKLDLFTLNASTGYNFAADSLNWSVISSSFR
                     TQVTKKIALNLNAT"
     assembly_gap    3242..3341
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3433..4227
                     /locus_tag="PLI55_07415"
     CDS             3433..4227
                     /locus_tag="PLI55_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPB00703.1"
                     /translation="MSQISNAKSLFNPAYDILALDFDGVIVDSIAECLVVGHNAFQSA
                     IGRSEKINDLSQLPREIQQESRRLRNFIRHGEDYVYIFKAIADDFSIHNQADFDDYIE
                     KNIDVKEKFRTQFYLERARFLREEPLRWLALNPFYDGMADFLKNFSPPEHLYIITTKK
                     LEYVQVILDTAGIPLQRKNLFAANSQKGKPEIIADLLQKTRLAPDSFYFIDDQVDTLI
                     KLKPLKIRLFVAGWGYTNQEQIRQAETEGIPTLTLNEFYQLFSRSD"
     gene            4567..5577
                     /gene="gap"
                     /locus_tag="PLI55_07420"
     CDS             4567..5577
                     /gene="gap"
                     /locus_tag="PLI55_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPB00704.1"
                     /translation="MAIKVAINGFGRIGRLVLKASYKNPQVDIVAINDLTDAKTLAHL
                     LKYDSVHGKFDGTVSVDGNYLVINGKKIEVFAQKDPSLLPWGKLGVEIVVEATGKFRN
                     REGMQKHITAGAKKVILTVPADSKEDVDVTIVPGVNDEMLTKNSIFVSNASCTTNCLA
                     PVAKVLNDNFGIKRGLMNTIHSYTNDQVILDFPHKDLRRARAAAVSIIPTKTGAAKAI
                     GLVIPALDKKMDGFAMRVPTPDGSVVDLTCELEKPATKEEINAAMKAAAEGPMKGILE
                     YTEDPIVSIDIVGNPHSSIFDAGMTKVLDGNFAKIISWYDNEWGYSCRVAELIVKMGL
                     LK"
     gene            5599..7719
                     /locus_tag="PLI55_07425"
     CDS             5599..7719
                     /locus_tag="PLI55_07425"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HPB00705.1"
                     /translation="MKTVNFIFGIHNHQPVGNFDFVMESAYRQSYLPFIEIVEEFSAI
                     HITFHFSGCLLEWIEAHHPDYLDRIAALVKRGNVEIISGGFFEPVLAMIPDADKVGQI
                     KMMNDYIQQRFNYTAKGLWLTERVWEPHLAKPIAEAGIKYITVDDYHFLSNGKQESEL
                     TGHFLTEEQGKTLGIFPISQRLRYTIPFQDPQATIDHLAKFATDDGENVIVMADDGEK
                     FGVWPGTHEQVFTKGWLRNFFAALLKNSEWLKTLTFAEWYENHPPKGRIYLPTASYFE
                     MSEWALPWEAGEKFADLVHEFESKKRIEEVRPFIKGGIWRNFLYKYDESNWMQKKMLH
                     LSERFADIDPTSLTKPQQVELGQARLHLWRSTCNCAYWHGIFGGLYLPHLRHAIYTEL
                     LICERLLDKITGAKGFRAEVFDFDTDGENEIIIKYGNLHTAIAPHRGGMITELSLVNK
                     AFNLLNNIQRYHESYHRKVALADKVENSNASGSIHDLVLSKEKGLDKYLKYDKYPRKN
                     LLDHVIHPAVSLERFRDGDYYEDSDFITGHYSPELNEKTRTLTLSKDGWVNWQRFRIK
                     KTITFGKEALKVTYILTNIGERENVFRFGPEFNFALLGGNSPDRYYIADGEKTAEPAL
                     NSIGILTGIKSLGVVNEWDKFQAEVIAPQAVEFWRFPIETVSLSEAGFERIYQSSVII
                     PWFDVRLNPEETATFSIELTTTEL"
     gene            8139..9083
                     /locus_tag="PLI55_07430"
     CDS             8139..9083
                     /locus_tag="PLI55_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HPB00706.1"
                     /translation="MKKHPTKLVKIDPIPILLERAPLPIRYFTAERFLSHDNELVEQL
                     RRDLQFFKPRERLLATQQEDGLWKLNKNYKIEEQQKSMQFLLQLQNMMQLLDYGCTKD
                     MPAIQRGLIAILKYQKPDGKFPLLQHHQGLTLWLLVRYGLAGNPFVEKGFRWLAKRQR
                     EDGGWLSPTMLATGESAKTARSGIWTTLVITQAFSAHSRLRNTETCQRAANFLLNVYL
                     QQNHTTLFPEPDAWNHLYIDYSENGLFRGGTLRFLETLTPLPNCHSHPNFKKALDWLI
                     GQQLPSGLWPAIAGRSKEGDYAVTFRVMVVLSEIDRIG"
     gene            9117..11489
                     /gene="lon"
                     /locus_tag="PLI55_07435"
     CDS             9117..11489
                     /gene="lon"
                     /locus_tag="PLI55_07435"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPB00707.1"
                     /translation="MAETIKNKDEKNETGHGVPEILPIMALRNTVLFPQQVIPIYIGR
                     ERSLRLIEDLPETKKRIVVVAQKEGTLENPEVDDLYEWGTIAQVLKIFNMPDGSKSAI
                     VQGLERAKIQSFIQVEPYFKGAILKAQEIYQPGIEIDAMLANLRQLYQKLIKIAPYLS
                     EEQASVFSSVQHPGRLIDRIIHLLNVPVSEKQEILEELDVRKRLEKATVILNREIQRI
                     QLGEQIQSEVQDEISKTQREYFLREQLKAIRKELGEDEGTLELKELEEKINLSKMSDE
                     ARKVAEKELDRLRKIPPASPEYTVSRTYLDWLVDLPWGIYTTDNVDIKSAQKILDEDH
                     YGLEKIKDRIIEYLAVRKLKQEQDPNASIKGPILCFVGPPGVGKTSMGKSIARAMGRK
                     FVRISLGGVRDEAEIRGHRRTYIGALPGRILQSIRKAESSNPIFMLDEIDKIGADFRG
                     DPSSALLEVLDPEQNSTFSDHYLEVTYDLSKTMFIATANMVDTILPALRDRMEILEFS
                     GYIEEEKLNIAKTFLIPKQIKEHGLTNVTVEFTDKAIRFIINSYTREAGVRNLEREIA
                     NVCRKIAREVAVNKRKKNFLIDEKAVENFLGPQRYFVDIAERLSKPGVAIGLAWTSVG
                     GDILFVEATSMRGTGKLNLTGQLGDVMKESAEAALSYIRSNATLLGLDEEAFSKIDIH
                     IHVPAGAIPKDGPSAGVTILTAVYSLLKKRKVKDNLAMTGEITLRGAVLPIGGVKEKV
                     LAAHRAGLRRVILPAQNRKDLIEIPKQIKNEMRFDFVKEMSEVLNLAIRK"
     gene            11539..11612
                     /locus_tag="PLI55_07440"
     tRNA            11539..11612
                     /locus_tag="PLI55_07440"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11573..11575,aa:Val,seq:tac)
BASE COUNT         3413 a         2478 c         2516 g         3106 t
ORIGIN      
        1 taattaaacc taagaacaaa ggaaaagaat ttttatattt aatattgatg tgttttcagg
       61 taaatggtag atattggaat agtgtaagga ttaatttcca gcaatcactc ttgaaaatta
      121 ctcgactggg tgtaataatt agtttgctaa ttatgctgat tggattggtg ttctttgatt
      181 cggccgacgc cgtcgaaaaa tatgagcctc tgcgtctcat tcgcgccgac aagctcgagc
      241 agttttatcg cgccgataca ccgattaaaa gactgaccgg caatgtcctt tttcagaaag
      301 gtggattgca attggcttgc gatctggctt attggttcga aagggatcag cgcgctgatt
      361 tctatcgcaa cgtcatcgcg accagtgata ataaagtcct gaaggctgat tcgctggttt
      421 atttcagcga aaatgattcg atcgtcgccc gtggccaccc gattctatat gacagcactt
      481 tgtcaataac agcccgtcgc ttgatatatt tagtcaaaga cgaaatcgct catgccgagg
      541 ggaaggttct actcaaggac agcagtcgga gcgttcgtgc taacctgatt cactattatt
      601 ctaacgaaaa gaaatcagtc gccatcgggc aggctgtttt atacgatgtc aagcgacacc
      661 tggagctggc ttccgacagt ttgatttatt tcaataagac tggcaatatt tttgccttga
      721 accgaccgat gctaacccgt tttgatactg ctggtgtcga atcgtttcgg atttacgccg
      781 ataccattcg cctcgttgaa aaagaaggta attttcaggc aattggcaac gtgcgcattc
      841 agagccagga ctttaccgct tattctaaaa ctttgaccta tattgattcg ctggaagtcg
      901 ccactttgac cgataatcca cgggtgttga gcgacggcca ggaattgacc ggcaaagtta
      961 tgcgcttaca ctttgagaat gaaaaaattt cagcgttgta tattgacgaa gatgcacgtg
     1021 ccaatgccgt gggcaaggct tatttgccag ccgagtccga tagtctcgct catggcgatt
     1081 ctattaaaac ttatgacgaa atcactggca agttcatgga aatttatttt cgagacgatc
     1141 aagccgattc gatcgtggtt gccgttatgg cgaccagcct gtataacatg ctggaggaca
     1201 gtatcattct gggcattaat gaagtcagcg gcgacacaat ccggttaaaa ttcattgacc
     1261 ggaaaatcaa tacaatcacc gttgtcggcg gcacgcaggg acgctttatt ccgcacgaaa
     1321 ccaacaccga cttggatacc accgttatct atagcgccga gcggatagac tattttgttg
     1381 atcgcaaact gaccgatctt tatctaaacg ccagaattca gtatgccgat atcgaattga
     1441 ccgccggcaa gattaatatc aattggaatg aaaatttatt gtatgcatat ccacttagtc
     1501 ccccgcctta cgattccacc agcgttgatt tgccggtcct aaaccagcgc gggcgagaac
     1561 catttgtcgg cgaaagtatg gtttataatt taaaaactca gcgcggtcgc atcgttagtg
     1621 gcaaaaccaa ggaacaggac ggttattatt atggcgataa aatcagtaaa gtagataaaa
     1681 aagtctttta cgtcaatgat tgcgtttaca cgacttgtga tattccggat aagccacatt
     1741 attatttccg cagtaaacaa ctaaaaatta tctttaaaga taaagtgatc gctcggccgg
     1801 taatttttta tattcatgat ataccgcttt tgggattgcc gtttattatt attccggatc
     1861 agagcggcaa gcgtcattct ggctggatta tgccctccta tggcgaaagc cgaatcggcg
     1921 gtggattcat cagaggactg ggctattttt gggcgccgaa cgattattac gatttccgtc
     1981 tgactggtga tttttacgat aaacagggcg ttattctcga ctatcgccta cgctatgcct
     2041 ggcgttataa actgaatggc accatcagcg gtaaatacac caatgacttt ttcagcaact
     2101 atcctgagaa gcaatggact ttcaatatta ctcacaatca aacgatcgat ccgacaactc
     2161 gcctaacagt caacggcaaa tttgtcagca atgaaagtct atataaaaaa tacagctatg
     2221 atctggaaga ccgcctcgac cagcaattga ttagcaatgc cacgctctcc aaatcatgga
     2281 cggggaaacc atattcattg tcagttaatt tcaatcaaac cactaacctg caagccaatg
     2341 ctctcagtca aatcgaaccg accgattcca acttgaccat tgcgcacatt agtcgttcat
     2401 tgccgaatat ttctttcacc cgaagcagta agcctttgat tcccctcaag ccgcatcagg
     2461 atgtttctcg atctaaatgg tataataata tttacttcag cgccagctcc tatctaaaaa
     2521 ataaccagaa tattttttac cagagcattt ataatcctgc taccgattca ctggaatggc
     2581 aggagcagaa catcgcaaaa aatgccgtta cgtataactt ttcattcacc agttcccaga
     2641 aaattttcgg ctttttaact accaatcaaa cggtcaacct cgatgaaggt tgggttttca
     2701 agtacgatat gccactttat gacgaagacc accgctttca gctggaaaac ggtaaaataa
     2761 aaaccgaaac cgtagaaggg tttaaagccc gccataccgg tagcgccaca atcagtgccc
     2821 aaaccaaagc ttatggcctg tttcccgtca ggattgccgg tttgcaggct gttcgccatg
     2881 tcgttacgcc atcggtcagt atgacattta ggcctgactt cagccgagaa atttttgggt
     2941 gggatccagg ctatatcatt cgcggaattg atacaaccgg cactgtccgg actttcgacc
     3001 cactgactaa tactatgctg ggcgccttgt cttcttatga aacccgcaca atgtctatga
     3061 gtctcagtaa tcttttccaa gctaaactgg gacaaggcga aaaaactcgc aagcttgatc
     3121 tttttactct gaatgcttcg accggctata attttgccgc cgatagtcta aactggtcag
     3181 tgatttcgag ctccttccga actcaggtta ccaagaaaat agctctcaac ctgaacgcaa
     3241 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatatcaaa tatgaagagc
     3361 gcggtgggcg ccgcagtagc ctgggtttct aaaaagaata tgaaattata attgaaccaa
     3421 agtaactaaa aaatgtcaca aatttccaac gctaaatctc tttttaatcc agcttatgat
     3481 attctggcgc tggatttcga cggcgtcatc gttgatagca tcgcggaatg tttagtcgtc
     3541 ggacacaatg ccttccaaag tgccatcggc cgttcggaaa agatcaacga tctaagtcaa
     3601 ttgccgcgcg aaatccaaca agagagtcgc cgcttacgca attttattcg gcacggtgag
     3661 gattatgtct atatatttaa agcaattgcg gatgactttt ccattcataa tcaggctgat
     3721 ttcgatgact atattgaaaa aaatattgat gtcaaagaaa aattcagaac gcagttttac
     3781 ctcgaacgcg ctcgctttct gcgcgaggaa ccactgcgct ggctggctct taatccgttc
     3841 tatgacggaa tggcggattt cctcaaaaat ttctcaccgc cggaacattt atacataatt
     3901 actaccaaga aactcgaata tgttcaggta attttagaca cagccggcat tcctcttcaa
     3961 cggaaaaatt tattcgcggc taatagccaa aaaggtaaac cagaaataat tgccgatctt
     4021 ttacaaaaaa cgagattagc accagatagt ttttacttta ttgacgacca ggttgatact
     4081 ttaatcaaac tcaagcccct aaaaattcgc ctgtttgtag ccggttgggg atatacgaat
     4141 caggaacaaa tccgccaggc tgaaactgag ggcattccga ccttgacttt gaatgaattt
     4201 tatcagctgt tttccagatc ggattgacat aattttcggt catttctctt gtttttaaat
     4261 tttgacggct aaatgattcg attgacagtt ctataagaat gaaaataagg cgctttttga
     4321 aaagtaataa atcagaaaac aagttattgg caaaaaaata ttgatgagga aatggaaaaa
     4381 taggctgggg tattagtgat ttcccgagaa ttggcggaat ttctaaagaa ttaaagtcca
     4441 tttcccacaa tttttttgga agtaaattgc tggaaaatta aatttcaggt cggctttttg
     4501 aaaattaata atcattttta aaaatcatcc gaacaaattg aaaaataaaa caaaaggagt
     4561 ataattatgg caattaaagt cgcaatcaac ggatttggtc gcatcggacg tctcgtctta
     4621 aaggcttcct acaagaatcc tcaggttgat atcgtcgcaa tcaatgattt gaccgatgct
     4681 aagacattag cccatttgct aaaatacgac agcgttcatg gcaaattcga tggtaccgtc
     4741 agcgttgacg gcaattattt agttattaac ggaaaaaaga ttgaggtttt cgcacagaaa
     4801 gatccctcct tattgccgtg gggcaaattg ggtgtggaaa ttgtcgtcga agccaccggc
     4861 aaattccgca atcgtgaagg catgcaaaaa cacatcacgg ccggtgccaa gaaagtgatc
     4921 ctgactgttc cagctgactc caaggaagat gtggacgtaa ctatcgttcc aggtgtcaat
     4981 gacgaaatgc tgacgaaaaa ttcaatattc gtctcgaatg cttcttgcac gactaattgc
     5041 ctcgccccgg ttgctaaagt tctgaatgac aatttcggca tcaagcgcgg tttaatgaat
     5101 acaattcatt cttacacgaa tgatcaggtc attcttgatt ttccgcataa agacctgcgt
     5161 cgggcgcgcg ccgcagcggt gtcaattatc ccgaccaaaa ccggcgcggc caaagctatc
     5221 ggcctcgtta tcccggctct tgacaaaaag atggacggct tcgctatgcg cgttccgact
     5281 cccgatggtt ccgttgttga tttgacttgc gaactggaaa aaccagccac caaagaagaa
     5341 atcaacgccg ctatgaaagc cgccgctgaa ggcccaatga aaggcattct cgaatatact
     5401 gaagatccta tcgttagcat tgacattgtc ggtaatcccc actcttcaat tttcgacgcc
     5461 ggtatgacta aagttcttga cggcaatttc gctaagatca tctcctggta cgataacgaa
     5521 tggggttaca gctgtcgggt ggccgaatta attgtaaaaa tgggcctttt aaaataaaac
     5581 tgtctcaata gcatacattt gaaaaccgta aattttatct tcggcattca taaccatcag
     5641 ccggtaggta acttcgattt cgtgatggaa tctgcctacc ggcaaagtta tttgccattt
     5701 atcgaaatcg tcgaagaatt cagcgctatc cacattactt ttcacttcag cggttgtctt
     5761 ttggaatgga tcgaggcgca tcatccggat tacctcgatc gcattgccgc cctggttaag
     5821 cgcggaaacg tcgagattat ctccggtgga tttttcgaac cggtgctggc tatgattccc
     5881 gacgccgata aagtcggtca aattaaaatg atgaatgatt atattcaaca gcgttttaat
     5941 tatacggcta aaggactgtg gctaacggaa cgagtctggg aacctcatct tgccaaacca
     6001 attgccgaag ctggaataaa atacatcacc gttgacgatt atcatttttt aagcaatggt
     6061 aaacaggaaa gcgaactgac tggccatttt ctgactgagg aacaaggcaa gaccctcggt
     6121 attttcccaa tcagtcagcg attgcgctat accattccat tccaggatcc gcaggcaact
     6181 attgatcatt tggctaaatt cgccaccgac gacggtgaaa atgtcattgt tatggcggat
     6241 gacggcgaaa aattcggcgt ctggcccgga actcacgagc aggtttttac caaaggctgg
     6301 ctgaggaatt ttttcgcggc tttgctgaaa aatagcgagt ggctcaagac tttgaccttt
     6361 gccgaatggt acgaaaatca tccgcccaaa ggccgcattt atctaccaac ggcttcctac
     6421 tttgaaatga gtgagtgggc tctgccgtgg gaggctggcg aaaaatttgc cgatttagtc
     6481 cacgaattcg aaagcaagaa gcgtattgag gaagtccgac cttttatcaa aggtggcatc
     6541 tggcgcaact ttctctataa atacgacgaa agcaactgga tgcagaagaa aatgttgcac
     6601 ctttcggaac gctttgccga tattgatccg acgagcctaa caaagcctca gcaagttgaa
     6661 ttggggcaag cccgtctgca tctctggcgt agtacctgta actgcgccta ctggcatggt
     6721 attttcggcg gactttatct gccgcacctg cgccatgcaa tttataccga actgctaatc
     6781 tgcgaacggc tgctagataa aatcacgggt gcaaaaggct tccgagcgga ggtgtttgat
     6841 ttcgataccg acggtgaaaa tgaaattatt ataaaatacg gcaaccttca tactgctatt
     6901 gcaccgcatc gtggcggcat gataacggag ttatcgctgg taaacaaagc cttcaatctt
     6961 ttgaataaca ttcagcgtta tcacgaatcc tatcaccgca aagtggcatt agccgacaaa
     7021 gtagaaaatt ccaatgccag cggcagtatc catgatctgg tactttccaa ggaaaaggga
     7081 ctggataagt acctcaagta tgataaatac ccacgaaaaa atctactgga tcacgtgatt
     7141 cacccggcgg tttcgctgga gcgcttccgt gacggcgatt attacgaaga cagcgatttt
     7201 atcaccggcc attactctcc ggaactcaat gaaaaaaccc ggacgttgac tctctccaaa
     7261 gacggctggg taaattggca aagattcagg ataaaaaaga cgattacttt cggaaaagag
     7321 gcactgaaag ttacctacat attgaccaat atcggcgaac gtgagaatgt tttccgcttc
     7381 ggaccggagt ttaactttgc tcttctgggc ggcaattcgc ccgaccgtta ttacatcgcc
     7441 gacggtgaaa aaaccgccga accggcgctg aactcgatcg ggattttaac cggtattaag
     7501 tctctgggtg ttgtcaatga atgggacaaa ttccaagccg aagtaattgc gccccaggcg
     7561 gtcgaattct ggcgcttccc gatcgaaaca gtctcgctct cggaagccgg attcgagcgc
     7621 atttatcaaa gttcagtaat aattccgtgg ttcgatgtca gattgaaccc ggaagaaact
     7681 gccactttct cgatcgaatt aacgacgact gaattataga agtttaaact atattaactc
     7741 gattagaaga aaatgactat gataagtagg atttttcata ttcaattatt ccgattatat
     7801 gtttagataa attcaatgaa aattaatttg acaaatataa aattataagc aagatatctc
     7861 atagggaaag tttagagtac taataaattg gatactatga cttttgatcc gctaaaaaat
     7921 acttgcattt caagccgatt aagaggatat taaggcgctt tttggttttt tcgatattta
     7981 aaatgagaac aagactcatc tgttatacag aacccattta taaaagggcg attttagcga
     8041 aattgcccga acaattaaat ccaacaatta atacaaaatg tatccgtagc aaacgcttaa
     8101 agatcacttt ttaatatgat aaaggcttag aatcctatat gaaaaaacac ccaactaaat
     8161 tagtaaaaat cgatcccatc cctattcttt tggaaagagc tccccttccg atccgatatt
     8221 ttacggctga acgtttctta tcgcacgata acgaattagt cgagcaactt agacgggatc
     8281 tgcaattctt taaaccgcga gaacgtctgc tagctactca gcaggaagat ggtctctgga
     8341 aactgaacaa aaattacaaa atcgaagagc aacagaaatc aatgcagttt ctgttacagt
     8401 tgcagaatat gatgcagtta ttggattatg gttgcaccaa agatatgcca gctattcaga
     8461 gaggcttaat cgctattttg aagtatcaga aaccggatgg aaaatttcct ttactccaac
     8521 atcatcaggg cttgacttta tggttattgg ttagatacgg tttagccggt aatccatttg
     8581 tcgaaaaggg cttccgttgg ttggcaaagc gtcaaagaga agatggcggt tggctctcac
     8641 ctactatgct agccacaggc gaatcggcga aaactgctcg cagtggaata tggactactc
     8701 tggtaattac tcaggctttc agcgctcatt cccgtttacg taacactgag acttgccagc
     8761 gcgccgcaaa ttttttgctg aatgtgtatt tacagcagaa tcacacgacg cttttccccg
     8821 agccggatgc ctggaaccat ttatatattg attattctga aaacggctta ttccgcggcg
     8881 gcacattgcg gtttctggaa accctgaccc ctttgccaaa ttgccattct catcctaact
     8941 ttaaaaaggc gttggactgg cttatcggtc agcaattgcc ttccggtcta tggccagcta
     9001 ttgccggacg ttccaaagaa ggcgattatg ctgtaacttt tagagttatg gtggttctaa
     9061 gtgaaataga ccgaattgga taatgacata aagtgtttat ttgaaaggta ttaaatatgg
     9121 ctgaaacaat aaaaaataaa gatgaaaaaa atgagacggg gcatggtgta ccggaaatat
     9181 tgccgataat ggctctgcgt aataccgtgc tttttcctca acaagtcatc cctatttata
     9241 ttgggcgcga acgttcattg cgcttaatag aggatttacc cgaaactaaa aaacggatcg
     9301 ttgtggttgc tcaaaaagaa ggtactttag aaaatcccga ggtagatgat ttgtatgaat
     9361 ggggaacgat tgcccaggta ttaaaaatat tcaatatgcc ggatggcagt aaaagtgcaa
     9421 ttgttcaggg gctagaacgc gccaaaattc aatcatttat tcaggttgag ccttatttta
     9481 agggagccat cctcaaagca caggaaattt atcagccagg catcgaaata gacgcgatgc
     9541 tggctaacct ccgtcaacta tatcaaaaat tgattaaaat cgccccttac ttaagtgaag
     9601 aacaggcttc ggtattttcc tcagttcagc atcccggccg tctaattgac cgaataattc
     9661 acttgctcaa tgtgccggta agtgaaaaac aggaaattct ggaagaattg gacgtccgta
     9721 aacgtctgga aaaagctacc gtaattctta atcgagaaat ccaacgcatc cagctagggg
     9781 agcaaattca gtctgaggtt caggatgaaa tttccaaaac tcagcgcgaa tattttctgc
     9841 gggaacagct gaaagccatt cgcaaggagc tgggcgaaga cgaaggcacc ttagagctaa
     9901 aagaattaga agaaaaaatt aacctatcaa aaatgagcga tgaagcccgt aaagttgctg
     9961 agaaagagct cgaccggctt agaaaaattc caccagcctc accagaatac acggtttccc
    10021 gcacttactt ggactggttg gtagatttgc cctggggcat ttatacaacg gataatgtag
    10081 atattaaaag tgcgcagaaa atactcgacg aagatcacta tggtctggaa aaaatcaaag
    10141 accgaattat cgaatacctg gcagtccgca aattgaagca agaacaggat cccaatgcta
    10201 gtatcaaagg cccaatcctt tgttttgttg ggcctcctgg tgtaggtaaa acctcgatgg
    10261 ggaagtcaat tgcccgcgct atgggtcgca aattcgtccg catttcgctc ggaggagtac
    10321 gggatgaagc tgaaattcgc ggccatcggc gcacctatat cggcgctttg cccggccgta
    10381 ttttgcaaag cattcgtaag gcagaatcga gcaatccgat ttttatgctg gatgagattg
    10441 acaaaatcgg agcggatttc cgcggtgatc cgagttctgc tttactggaa gtccttgatc
    10501 ccgagcaaaa ttcgactttc agtgatcatt acctcgaagt tacctacgat ctctctaaaa
    10561 ctatgtttat tgccactgcc aatatggtgg acactatttt accagcctta agggatcgaa
    10621 tggaaatact ggaatttagc ggctatatcg aagaagagaa gttgaacatt gccaagactt
    10681 ttttaattcc aaaacagatt aaggaacatg gcttaactaa tgtcacagtt gagtttactg
    10741 ataaagcaat acgttttatt attaatagct acacccgaga agcaggagtt cgtaatctcg
    10801 aacgggaaat tgccaacgtt tgccgcaaaa tcgcccgtga agtcgctgtt aataaacgca
    10861 aaaaaaactt tctaattgat gaaaaagccg ttgaaaattt tcttggtcca cagcgctatt
    10921 ttgtggatat agccgaaaga ctctccaagc ctggtgttgc tatcggattg gcttggactt
    10981 cggttggcgg cgatattctt tttgtagaag ccaccagtat gcgaggcacc ggcaagttga
    11041 atctcaccgg tcagcttggt gatgtaatga aggaatcagc tgaagccgca ctcagctata
    11101 tccgttcaaa tgcgacttta ttgggactcg atgaggaagc tttctcgaaa atcgatatcc
    11161 atattcacgt gccggctggc gcaataccga aagatggccc atcggccgga gtaacgattt
    11221 taacggcggt ttattcattg ttaaaaaagc ggaaggtcaa agacaatctg gctatgaccg
    11281 gtgaaattac tttacgcggg gcggttctgc caattggtgg cgtgaaagag aaagttcttg
    11341 ccgctcaccg ggcgggattg cgccgggtaa ttttaccagc ccagaatcgc aaggacttaa
    11401 tcgaaatccc aaagcagata aaaaatgaga tgcgatttga ttttgtaaag gaaatgtcag
    11461 aagttctcaa ccttgccatt agaaaataaa tgtcggttta aattttattg cgtaaatttg
    11521 cccgctctta agtaaaatgg acgcttagct cagctggtca gagcatcgcc cttacaaggc
    11581 gggggtcgta ggttcgaatc ccacagcgtc cac
//
LOCUS       DAOOMU010000071        11509 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_6285_length_11509_cov_15.408067, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000071 DAOOMU010000000
VERSION     DAOOMU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11509
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6285_length_11509_cov_15.408067"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLI55_07445"
     CDS             <1..615
                     /locus_tag="PLI55_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPB00708.1"
                     /translation="LGQWDVKRLLAYHSISQIGYIIIGVGLGTTLGLLGAVFHLLNHA
                     IFKALLFLNAGSLEMALGTRDLRQMGKVSKALPVTSFTSMVASLSISGIPPFNGFFSK
                     LIIFIAAIQVGRPVVAFLAGLGSLLTLASFMKVQRYGFHGEKTVTADQAKVGWGMRAA
                     MIVLAILCLATSALVIPGVREVTLDPVVEVIMNKTGYITRVLGR"
     gene            618..1124
                     /locus_tag="PLI55_07450"
     CDS             618..1124
                     /locus_tag="PLI55_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HPB00709.1"
                     /translation="MTIKNRSRVIVFVLSFLVWLALTDIKDIQEVVAGLIVAFLVSLI
                     AGTFLVTTEKSRHPVRRFFMGIVYLFKFLWEMIKANIHVALIVINPTLPIRPGIIKIK
                     SNLTKDSALTVLTNSITLTPGTLTVDINPETREIYIHCIEVESTAIDENTRSIGGRFE
                     KLIREVFE"
     gene            1121..1459
                     /locus_tag="PLI55_07455"
     CDS             1121..1459
                     /locus_tag="PLI55_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HPB00710.1"
                     /translation="MRVLRWVIGLVLIGAFMYANFFIYTASIYVKLINVILFSALFVI
                     YRVIVGPTAADRIVAVDILGILIIGMLALLGLNYDQSFFMDIGLIWALLSFIASLAFA
                     KVLEGRSLDD"
     gene            1452..>1702
                     /gene="mnhG"
                     /locus_tag="PLI55_07460"
     CDS             1452..>1702
                     /gene="mnhG"
                     /locus_tag="PLI55_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HPB00711.1"
                     /translation="MIRTLIGMAFILIGVTFDFLGVLGLVRLPDVYNRLQAATKCVTF
                     GSAGLLFGVFILQGFNSFGFKVLIGIVFIFLTSPVAAHV"
     assembly_gap    1703..1802
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1803..2164
                     /locus_tag="PLI55_07465"
     CDS             <1803..2164
                     /locus_tag="PLI55_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HPB00712.1"
                     /translation="CAQCGQCQEKCITGKGIQLSKNYLLAISDLKDQAVYNTVEKDLA
                     LCEACGTKIACFDQLLFVKERLGAKSYANPNLLLVTQQQFSEVPDSHVKDRLRREDYV
                     KMVCPKCRHKIVVKDEF"
     gene            2154..2621
                     /locus_tag="PLI55_07470"
     CDS             2154..2621
                     /locus_tag="PLI55_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HPB00713.1"
                     /translation="MNFNDLKKTLAVYHSDRIIFVGLGNRDCGDDGASQILLNKLRHS
                     EEFKEAGFIFAGTNPENYLQKITDKNPQAVVFIDAAHFQDQSGTVKFLEAKQLKPSDF
                     STHAFSISLIAEYLTYEKSCAIYYLGIQPENSGVGQSLSESVQSGLAHFFSES"
     gene            2632..>2966
                     /locus_tag="PLI55_07475"
     CDS             2632..>2966
                     /locus_tag="PLI55_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00714.1"
                     /translation="MATNEPYKLILPFHTLETMQTRISWLVNLRWIGIISLLGIIPIA
                     EQVLKFSLGFPQIYTLTVVFIVLNVTYFFICKYFKFVNFGQEIAFTEIQIIFDFVLIS
                     FVVHYTGGI"
     assembly_gap    2967..3066
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3067..3681
                     /locus_tag="PLI55_07480"
     CDS             <3067..3681
                     /locus_tag="PLI55_07480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB00715.1"
                     /translation="VYSDSLQPELKEMVERAVRRTDQVLMMVKEMIEVTQYRYGLMEQ
                     KLDKVNFQEWIDKVVEQQQIYAANKKIALIVKKLPQSPAIYIDTVSMEKVLSNLINNA
                     IRYSPAGAEVTILPFVKQQCFGFCVRDTGIGIAPDELPKIFEEFYRSANAREMENLGT
                     GLGLSLVKQIVEKYNGKIFVESKIGQGSTFTVEMPYLNLQINPK"
     gene            3736..4158
                     /gene="nikR"
                     /locus_tag="PLI55_07485"
     CDS             3736..4158
                     /gene="nikR"
                     /locus_tag="PLI55_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPB00716.1"
                     /translation="MAGLIRFGVSLPEDLLTRYDNEIQRRGYSNRSEALRDLIREFLV
                     QKEVDSDAEVVGTLTLIYDHHVADLAAKLKSMQHESYQNIMANIHFHLDQHNCLEVIV
                     LRGRCSLIQNIAERLIGLRGVKHGKLTFTSTGKNLPGL"
     gene            4175..6040
                     /locus_tag="PLI55_07490"
     CDS             4175..6040
                     /locus_tag="PLI55_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB00717.1"
                     /translation="MAIKYALKYFLLITLMAVSLSSAAIYTDTLKVFPFDPVVVTGSR
                     TAINRHELPVAITVIPENVLSEQSGAPLLDLLSENVPGLFITRRTNLGYGLSSGAGGQ
                     ISMRGIGSFPTTQVLVLIDGRPDIMGLFGHPLGDVYFAENVKKIEVQRGPASLLYGSN
                     AMGGAINIITDHKSEPGYHLRMPIRFGSFNSHQLNLHQSFERKNWGVSGTVGAVSSDG
                     YRTDGRDDYSNQNGIAEAHWQIGNAWRADVNAYFSNMEIYDPGQTDNPFSNHRYDIKR
                     RGGDLTFNHSLTKLTSDLKLHYNYGHHDIDDPNKYISDDYTAGLILTETFYKSEKSRV
                     MVGIDLRQYGGKAWLDSTWKKHNVAEQSLITQFNRKLFSLVNLDGGLRYTHHSVAGAE
                     WIPALGMALHLPKGWYLKTQFARGYRNPTINELYLFMPSTTDLKPELSTNLEFIVEKE
                     IIGWLTTSLSLYHTKAENLIEKGWVSGMPIYQNRGEVVLKGIEWEGQLIITSNFSTSW
                     TMSVNTASQKVSGNPGQKCDLGLRWNPTEKVHLTLQSQWINNLYSLANPYNYGQPVYK
                     RLNGYWLLNWRGSYVINQFIDLSAEIENLTNVEYETMYHYPLPGRNFWIGITLKY"
     gene            6037..6654
                     /locus_tag="PLI55_07495"
     CDS             6037..6654
                     /locus_tag="PLI55_07495"
                     /inference="COORDINATES: protein motif:HMM:NF017001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB00718.1"
                     /translation="MIYFRQMNTAIYKNIIKYFDAQAASWHLSGLDRIQAENILSSVQ
                     LPESGVVLDAGCGAGNLFPILRKIVPLATIIACDISRKMLIESRQRFSNNVPLWVGYC
                     EELPLKDASVDLILNYCIFPHIKNSYKALFEYRRVLKSGGHLLIVHPQGRQTINSKHR
                     QIGFPISDDLLPPSAEIVECLESMKFRIHKVVDREDLYLIEAEVA"
     gene            complement(6764..7483)
                     /locus_tag="PLI55_07500"
     CDS             complement(6764..7483)
                     /locus_tag="PLI55_07500"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPB00719.1"
                     /translation="MGSRIKTLLFLLSLLVILAMAKNPQYPDYYQLRQKMVNEQIIAR
                     GVRAESVIKAMQKVERHLFVPEQYRNFAYSDRPLPIGEGQTISQPYIVALMTELLDLK
                     KSDKVLEIGTGSGYQAAILAEICDSVYTIEIIPSLGKQAQALLRELGYHNIHCKIGDG
                     YLGWPEHAPYDGIIVTCAPSKIPQPLKEQLAEGGRMVIPVGATYTQELVLVTKTKGKL
                     IQKSVIPVRFVPMLRSPNGLD"
     gene            complement(7514..8209)
                     /locus_tag="PLI55_07505"
     CDS             complement(7514..8209)
                     /locus_tag="PLI55_07505"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPB00720.1"
                     /translation="MSKRKISGVKIAGLVLIILGVLLLLDNLNLIFFDLGDFLHNWWP
                     VFLILIGLNELLKTNHSGGWFLIGLGIILTLKINDIIDGSLLIAMLLILIGIIIIIRP
                     RRFYPQKTADNSQPETNNSNNINLRAVFSESTENIISDNFSGGKVETIFGKMVVDLRP
                     AKMQPGRYHLNVETVFGQTILILPNKISAEVIGTPVFGRIDNQAKAIPRDESTAVLEI
                     RAEVVFGKIEIRQ"
     gene            8400..10064
                     /locus_tag="PLI55_07510"
     CDS             8400..10064
                     /locus_tag="PLI55_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HPB00721.1"
                     /translation="MKKFILMLFMVLMMMNCDSKIKQTEQMAQAFIDSLTNVIKPLNK
                     AANLAYWEATASGEDEYYERYAGASLELATIYANSADFEKLKALKSSKIKDPLLARQI
                     EILYYRFLAKQIDTTLLKQITEMQTAVEARFNKFRGKIDGRAVTGNDIKEILVSSNDL
                     TLRKKAWEASKQVAPAVEKDMLALVHLRNEAARKLGFNNFQQMSLITDEQDPAEIERI
                     FQELDAATREPFRHDKAMMDSILSKRFNIKPEELRPWHYADPFFQDVPAVSAVNLDQY
                     YQNQNVVKIAESFYAGIGLDVSQILANSDLYERPGKYPHAYCTDIDREGDVRIMVNVR
                     NNEEWMATVLHELGHAVYSINIDRQLPYFLREEAHLFTTEAIAMFFEKLSKNPTWMRA
                     MLNLDAEEEADIREATAQMLRLQKLIFARWSLVMYNFEKSLYENPDQDLNQLWWDLVE
                     QYQMIKRPENRNQPDWASKIHVCSYPVYYHNYQLGELLAAQLTEAIARYQGVNDGAKI
                     KYVNNPDLGNYLKTNIFSVGRKYRWDEMVVRATGEKLTAKYFVEQL"
     gene            complement(10209..>11509)
                     /locus_tag="PLI55_07515"
     CDS             complement(10209..>11509)
                     /locus_tag="PLI55_07515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPB00722.1"
                     /translation="LFSNAEFPFPEIVLSDGTKVKLDLATFATLRALPVRADRELVFN
                     SFFGKMYDFRRTFGATMDANVKQHLFIAHVRNYNSCLETALDANNIPTDVYTALIKNV
                     NDNLPTFYRYLKIKQQLLGVDTLKYTDLYTPVVKDLDIDYPIETAQQMILSALKPLGS
                     DYLSVIERAFNERWIDVYPTPAKRSGAYSDGSIYDVHPYVLLNYCNKYDDVATLAHEL
                     GHTMHSYFSNKTQPFPLSDYSIFVAEVASTFNEVLLFHDNLNKVTDPDQKLTLLMNYL
                     DGIKGTVFRQTQFAEFELRMHEMAERGEQLTGDSLTELYKQIVRMYYGHDKGICYVDD
                     IINIEWAYIPHFYYNFYVYQYATSFTAATALAKKVLEGDESARSNYLNFLSSGGSDYP
                     INQLKAAGVDMTTAEPFNLTMQEMNWVMDEIEKVLAEKKN"
BASE COUNT         3193 a         2207 c         2594 g         3315 t
ORIGIN      
        1 ctgggtcaat gggatgttaa acgtctgctt gcctatcata gcattagcca gatcgggtac
       61 attattatcg gtgtggggtt aggaacgact ttaggtctat taggtgctgt ttttcatctt
      121 ttgaatcacg ccatattcaa ggctttgttg tttttgaatg ccggttcgct ggaaatggca
      181 ttgggtacgc gtgatttgcg tcagatgggc aaagtttcca aagctttgcc ggtgacgtcg
      241 tttacttcga tggtggcttc gctttcgatt tcaggaatac caccgttcaa tggtttcttt
      301 agcaaattga taattttcat cgccgccatc caggtcggtc gtccggtggt ggcgttttta
      361 gccggactcg gcagtttgct gacgttagct tcatttatga aagttcaacg ttatggtttt
      421 catggcgaga aaacagttac tgccgatcaa gctaaagtag gatggggtat gcgagcggct
      481 atgattgtgc tggcgatact ttgtctggca accagtgctc tggtgattcc tggtgtccgg
      541 gaagtgaccc tcgatccggt ggtagaagtg attatgaata aaaccggcta cataacgcgg
      601 gtgttaggga ggtgaaaatg accatcaaga atcgtagtcg cgtcattgtg tttgtgcttt
      661 cgtttttggt ttggttggca ttgacagata taaaagacat acaggaagta gtcgcaggct
      721 taatagtggc atttctggtt agtttaatcg ctgggacatt tctggttacg acggaaaaga
      781 gccggcatcc ggttcgacgc ttttttatgg gaattgtcta tttattcaaa tttttatggg
      841 aaatgattaa agccaatatt cacgtggcat tgattgttat caaccccact ttgccaatcc
      901 ggcctggcat tatcaaaatc aagagtaatt taaccaaaga ttcggcctta actgtattaa
      961 cgaattccat cacattgaca ccgggtactc tgacggtgga cattaatcca gaaactcgtg
     1021 aaatttacat tcattgtatc gaggttgaat caaccgctat tgatgaaaat actcgttcca
     1081 tcggcgggcg ttttgaaaaa cttatcaggg aggtttttga atgagagttc tgcgatgggt
     1141 gattggattg gttctaatcg gcgcctttat gtatgctaat tttttcattt atacggcttc
     1201 gatttatgtc aagttgataa atgtcattct tttcagcgct ttgtttgtga tttaccgagt
     1261 tattgttggt ccgacagccg ccgatcggat tgtagcagta gatattctgg gcatattgat
     1321 tattggcatg ctggcattac tgggcttgaa ttacgatcaa tcttttttta tggatattgg
     1381 attgatctgg gcacttttaa gctttattgc ctcgctggcg tttgccaaag tattagaagg
     1441 gaggtcgctc gatgattagg acattaatcg gtatggcatt tatcctgatt ggggtaactt
     1501 ttgattttct gggagtatta gggttggtgc gtttgccaga tgtttacaat cgtctgcaag
     1561 cggctaccaa atgcgttaca ttcggctcag ctggcttgct atttggagtt ttcattctcc
     1621 agggcttcaa tagtttcggt tttaaagtgt tgatagggat tgtatttatt ttcctgactt
     1681 cgccagtggc ggcgcacgtc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nncctgtgct cagtgcggcc agtgccagga aaaatgtatc acaggtaagg gcattcaact
     1861 ttcgaaaaat tacttgcttg ctatctcgga tttaaaagat caagccgtct ataataccgt
     1921 ggagaaagat ctggctcttt gtgaagcctg cggcactaaa attgcctgct tcgatcagtt
     1981 actatttgtt aaagaacggt taggtgcgaa atcgtatgct aatccgaatt tgcttctggt
     2041 aactcaacaa caatttagcg aagtacccga ctcgcacgtg aaagaccgcc tgcgccgcga
     2101 agattacgtt aaaatggttt gtcccaaatg tcgccataaa atcgtcgtaa aagatgaatt
     2161 ttaatgattt aaagaaaact ttagccgttt atcatagcga ccgaataatt tttgtaggat
     2221 taggtaatcg ggattgcggt gatgatggcg cgagccagat tttgctaaat aaattaaggc
     2281 acagtgaaga atttaaagaa gcgggattca tttttgcagg tactaatccg gagaattatt
     2341 tgcaaaaaat cactgacaaa aatccgcagg ctgtggtctt tattgatgcc gcccattttc
     2401 aggaccagtc cggaacggta aaattcctgg aagcaaagca gctgaaaccg tccgatttta
     2461 gtacccacgc cttttcgatt agcctaatcg ccgaatattt gacttacgaa aaatcctgtg
     2521 cgatttatta tctcggaatc cagccggaaa atagcggagt tggtcaatcg ctatccgaat
     2581 cggtccaaag cgggctagcg cattttttct cagaaagtta gaaatataaa tatggctacc
     2641 aatgaaccat ataaattaat tctgcctttt catactctgg aaacgatgca gaccagaatc
     2701 agctggttgg taaacttgcg ctggataggc ataatcagtt tattggggat aattccgatt
     2761 gccgaacaag ttttaaaatt tagtctggga tttccgcaga tttatactct gacggtggtt
     2821 tttattgttc tcaatgtgac ctattttttt atatgtaagt atttcaaatt tgtcaatttc
     2881 gggcaggaaa tcgcctttac cgaaatccag ataatttttg attttgttct gatttcgttt
     2941 gtggttcatt atacgggcgg tattgannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnngttt attctgacag tctccaaccg gaattaaagg aaatggtcga gcgagctgta
     3121 cgacgcactg atcaggtttt aatgatggtc aaagaaatga ttgaggtaac gcagtaccga
     3181 tatggcttga tggaacaaaa gctggataag gtgaattttc aagaatggat tgacaaagtc
     3241 gtcgaacagc aacagattta tgccgctaat aaaaagattg cattgatagt caagaaatta
     3301 ccccaatcgc cagcgattta tatagacacg gtcagtatgg agaaggtgct ttctaactta
     3361 atcaataatg ccattcgcta tagccccgcc ggagccgaag taacgatcct accctttgtc
     3421 aaacagcaat gtttcggatt ttgtgtaagg gataccggga tcggtattgc tccggatgaa
     3481 ctgcctaaaa tttttgagga attctatcgc agtgctaatg ctcgtgaaat ggaaaatctg
     3541 gggaccggtt taggattgag cctcgtcaaa caaatcgtcg aaaaatataa cggcaaaata
     3601 tttgtcgaga gtaaaatagg gcaagggagc acctttaccg tcgaaatgcc ctatttaaat
     3661 ttgcaaatta atccaaaatg atatattttt tgactcgttg tgttacaaat atgaaaatgt
     3721 tgctacgagg tgaatatggc tggcttaatt agattcggag tttctctacc ggaagacctg
     3781 ctaacacgct acgacaatga aattcaacgc agaggctatt ctaatcgttc ggaagccttg
     3841 cgtgacctaa ttcgggaatt tttagtccag aaggaagttg attccgatgc cgaggttgtc
     3901 ggaacattaa ccttgatcta tgatcatcac gttgctgatt tggcggcaaa actgaagtct
     3961 atgcaacacg agtcatacca gaatattatg gcgaatattc attttcatct cgatcaacat
     4021 aattgtctcg aggtaatcgt attgcgcggt cggtgttcat taatacagaa tatagccgag
     4081 cggctgatcg gtttgcgcgg ggtaaaacat ggcaaattga cctttacatc aacaggaaaa
     4141 aacctaccgg gtttataaca tcagggatta aaaaatggca attaaatatg cgttaaaata
     4201 tttcttatta attaccttaa tggcagtgag tttaagctcc gcggcgattt ataccgatac
     4261 gctgaaagtt tttccattcg atccggtagt ggtaaccgga tcgcgcacgg ccatcaatcg
     4321 gcatgaactg ccggttgcaa tcacggtcat tcccgagaat gtattgagcg aacaaagtgg
     4381 agcgccgctt ctggatttgt tatccgaaaa tgtgccggga ttgtttatca cgaggcggac
     4441 gaatcttgga tatggattga gcagtggtgc tggtggtcag atttctatga gaggtatcgg
     4501 tagttttccg actactcagg tgttggtttt aatcgatggc cgcccggaca ttatgggact
     4561 tttcggacat cccctgggcg atgtttattt tgctgaaaat gtcaaaaaga tcgaggttca
     4621 acgcggtccg gcttcccttt tatatggttc gaatgcgatg ggaggagcga ttaatatcat
     4681 cacggatcat aagtccgagc cgggttacca tttgcggatg ccaatacgat tcggcagttt
     4741 taattcacat caattgaatc tgcatcagtc gttcgagcga aaaaactggg gagtctcggg
     4801 aacggtcgga gcggttagca gtgacgggta tcgaacagat ggtcgagacg attattccaa
     4861 tcaaaacggc atagccgaag ctcattggca aattggcaat gcctggcggg cagatgtcaa
     4921 tgcctatttt tcgaatatgg aaatttatga tccggggcaa actgacaatc cttttagcaa
     4981 tcatcgctat gatatcaaac ggcgcggtgg cgatctgact tttaatcaca gtctgacgaa
     5041 gttgaccagc gatttaaaat tgcattataa ttatggacat catgatattg atgatccgaa
     5101 taaatacata tcggatgatt acacagccgg tttgatattg acggaaacat tttataaatc
     5161 tgaaaagtca cgagtaatgg tcgggataga tttgcgtcag tacggcggca aagcctggtt
     5221 ggattcgacc tggaaaaagc acaacgtcgc cgaacaaagc ctcattactc aatttaatcg
     5281 aaagttattt tcattggtta atcttgatgg cggattgcgc tatactcatc attcggtcgc
     5341 tggtgcagaa tggataccag ccctcggtat ggctctccat ttaccaaaag gttggtatct
     5401 taaaactcaa ttcgccagag gctatcgcaa tccgacgatc aatgaacttt atttatttat
     5461 gccgtcaacc accgatttaa aaccagaact atcgaccaac ctggagttta tcgtcgagaa
     5521 ggaaatcatt ggttggttaa cgacgtctct atcattatat catacgaaag ccgagaattt
     5581 aattgaaaaa ggctgggtct ccggtatgcc gatttaccaa aatcggggtg aagtggttct
     5641 aaaaggtatc gaatgggaag ggcaattaat tatcacctct aatttttcca cttcctggac
     5701 gatgagtgtc aataccgctt cccagaaagt gagcggcaat cccggtcaaa aatgcgatct
     5761 tggtttgcgc tggaatccga ccgaaaaggt acatctgact ttacagagcc aatggattaa
     5821 caatctatat agcctcgcaa atccgtacaa ttatggccag ccggtctata agcgcctaaa
     5881 cggttattgg ttattaaact ggcgcggtag ttacgtaatc aaccaattca ttgatctaag
     5941 cgccgaaatt gagaacctga ccaacgtgga atatgaaact atgtaccatt atcctttgcc
     6001 aggccgcaac ttttggattg ggataacctt gaagtattga tttattttag acagatgaat
     6061 acggcaattt ataagaatat aattaaatat ttcgatgccc aggcggctag ctggcattta
     6121 tccggattgg atcggattca agccgagaat attttgagtt cagtccaatt acctgaatcc
     6181 ggagtggtgc tggatgccgg ttgcggagct ggaaatttat ttccgatatt gagaaaaata
     6241 gtaccgctgg ctactattat tgcctgcgat atttccagaa aaatgctgat cgaaagccgt
     6301 cagcgtttct cgaataatgt accactctgg gtcggatatt gcgaagagtt gcccttgaaa
     6361 gacgccagtg ttgatctaat cctgaattat tgtatttttc cgcatattaa aaacagttat
     6421 aaagcgttat tcgaatatcg ccgcgtttta aaatccggcg gacatctact gatagttcat
     6481 ccacagggtc gccagacgat taattccaag catcgtcaaa tcggatttcc aattagcgac
     6541 gatttattgc caccttcagc cgaaattgtg gaatgtctgg aaagtatgaa atttcggata
     6601 cataaggtag ttgatcggga agatttgtat ttaatcgaag ccgaggttgc ttaaacgcaa
     6661 agaacgcaat gtgcaccgaa gaaattgcga aattgctaag ttattttaaa tgaattatgc
     6721 ttatgatact aaatagcttt cgagcggaat tttcaactat attttaatct aagccgtttg
     6781 ggcttctcag catcggcaca aagcgcaccg ggatgactga tttttggatt aatttgccct
     6841 tagttttagt gaccagaact agttcctgtg tataagttgc accaaccgga ataaccattc
     6901 gaccaccctc ggcgagttgt tctttgagtg gttggggaat tttagatggc gcgcaggtga
     6961 cgattatacc gtcgtacggc gcatgttccg gccagccgag ataaccatct ccaattttgc
     7021 aatggatgtt gtgatagccc agctctctaa gaagtgcctg agcttgcttt ccgagtgagg
     7081 ggatgatttc gatcgtataa acgctatcgc agatttcagc cagaattgcc gcctgataac
     7141 cactgccggt accgatttcg aggactttgt cgctcttttt taaatccagt agttcggtca
     7201 tcagagctac aatataaggt tgcgagatag tttgcccttc gccaatcggc aaaggacgat
     7261 cggagtaagc aaaattccgg tattgttcag gcacaaatag atggcgttca accttttgca
     7321 tcgcttttat gacggattca gcccggacgc cacgcgcaat aatttgctca ttcaccattt
     7381 tctgccgtaa ttggtaataa tcgggatatt ggggattttt tgccatagcc aatattacca
     7441 aaagacttaa taaaaatagt aaggttttaa ttctactacc catttttcta tcaatgaatc
     7501 ctgactattc acattattgg cgaatttcta tcttgccgaa aactacttca gcccgaattt
     7561 ctagtaccgc tgtgctttcg tctctcggta tggctttcgc ttgattgtct atccgtccga
     7621 aaaccggcgt gccgattact tcagctgaaa ttttattagg tagaatcaaa atcgtttggc
     7681 cgaacaccgt ttcgacattg aggtgatagc gccccggttg cattttagcc gggcgcaagt
     7741 caactaccat tttcccgaaa atagtttcaa ctttgccgcc gctgaagttg tctgagataa
     7801 tgttctcggt ggattcggaa aatacagctc gtaagtttat gttattgcta ttattggtct
     7861 cgggttgaga attatcggcg gttttctgag gataaaatcg gcgaggacga attattatga
     7921 ttattccgat taaaatcaat agcattgcaa ttaatagaga accgtcaata atatcattga
     7981 ttttcaatgt cagaattatt cccagtccaa ttaaaaacca accgccggag tgattcgtct
     8041 tgagtaattc gttcaaaccg attagaatca aaaatacagg ccaccaatta tgcaggaaat
     8101 cgcctaaatc gaaaaatatc agatttagat tatccaaaag taataaaact cctaaaatta
     8161 taagaaccaa tccggctatc tttactcccg atattttgcg cttactcata ataaaccttt
     8221 ctctcttttt ttcaattata cgaaatagtt taaactaaat caatcggaaa atcggttaat
     8281 tttggctggt taattcttga atcctcgtga ctttaatggt aaattaaaaa ccgaatatgc
     8341 acgatgaata ttataacaaa atgtatcagt agaaaatatc tgcgaggagt atatccgata
     8401 tgaagaaatt tatcttaatg ttatttatgg tgttaatgat gatgaattgt gacagtaaaa
     8461 ttaaacagac cgaacagatg gcgcaggctt ttattgatag cctgaccaat gttattaagc
     8521 cgttgaataa agcggctaat ttagcctact gggaagccac tgcttccggg gaagatgaat
     8581 attacgaacg atatgcaggt gcttctttgg aattggcgac gatttatgcc aattcagccg
     8641 atttcgaaaa gctaaaagcc ttaaaatcca gcaaaatcaa agacccatta ttagcgcggc
     8701 aaatcgagat tctttactat cgctttttag ccaaacaaat tgatacaacc ttgttaaagc
     8761 aaatcaccga aatgcaaacg gcggttgaag ctcgattcaa taaattccgt ggtaaaattg
     8821 acggtcgcgc agtcaccggc aatgatatca aggaaattct ggtttcgtca aacgacctta
     8881 cattacggaa aaaagcctgg gaagcctcca agcaggttgc accggcagtc gaaaaagata
     8941 tgctggctct ggtgcattta cggaacgaag ccgctcgcaa actcggattc aataactttc
     9001 aacagatgtc gctgattacc gatgaacaag atccagctga aatcgagcga attttccagg
     9061 aactggatgc cgcaacgagg gaaccattcc gtcatgataa ggctatgatg gattcgattc
     9121 tgtccaagcg tttcaatatc aaaccggaag aactacgtcc ctggcattat gccgatccct
     9181 tttttcagga tgttccggct gtcagcgcgg ttaacctcga tcaatattat cagaatcaaa
     9241 atgtggtaaa aatagccgaa tcattttatg ccggaatcgg tctggatgta tcgcaaattc
     9301 tggctaatag tgatttgtat gagcgtccgg gcaaatatcc gcatgcctat tgcactgata
     9361 ttgatcgtga aggcgatgtg cgcattatgg taaacgtgcg caataatgaa gaatggatgg
     9421 cgactgttct gcatgaactg gggcatgcgg tttattcgat caatattgat cggcaactgc
     9481 cttacttttt acgtgaagaa gctcatctgt tcacgaccga agccattgcg atgtttttcg
     9541 agaaactatc gaagaatccg acctggatgc gggcaatgct taacctcgat gccgaggagg
     9601 aagccgatat tcgggaggca accgcgcaga tgttaagatt gcaaaaactg atttttgccc
     9661 gctggtcgtt ggtcatgtat aattttgaaa aatctctgta tgagaatccc gaccaggact
     9721 tgaatcaact ctggtgggac ctcgttgaac aatatcaaat gatcaagcgg ccggaaaatc
     9781 gcaaccagcc ggattgggct tccaagattc atgtttgttc ttacccggtt tattatcata
     9841 attaccagct aggcgaactt ttagccgccc agttgaccga agcgatagcg cgctatcagg
     9901 gagttaacga tggcgctaaa attaaatacg tcaataatcc cgacctggga aattacctga
     9961 aaaccaatat tttttcagtc ggcagaaaat atcgctggga tgagatggtt gtccgagcta
    10021 ccggcgagaa gctgaccgcc aagtattttg tcgagcagtt gtaattttta gtaagttcaa
    10081 ctaagcacgt ttaagttctt ttaagcgctg ataagaaatt aaaagaccct gtgatattga
    10141 gttacattca tgcacttatg catttaataa gcttgtaatc aatatttctt tgtagaactt
    10201 aaaagcactt agttcttctt ctcggcgagg actttttcaa tttcatccat tacccaattc
    10261 atttcctgca ttgttagatt gaacggttcg gcagtagtca tatcaacccc ggcggctttt
    10321 aactggttga tcgggtagtc ggaaccgccg gaagagagga aattcagata gttggaccgg
    10381 gcggattcgt caccttctaa tacttttttg gctaacgcgg tagcggctgt gaaggacgtg
    10441 gcatattgat aaacgtaaaa attatagtag aagtggggaa tataagccca ttcgatgttg
    10501 atgatgtcat ccacgtagca aatacctttg tcgtggccat aatacatccg cacgatttgt
    10561 ttataaagtt cagttagtga atcaccggtc aactgctcac cgcgttccgc catttcatgc
    10621 atacgcaatt caaactcggc gaactgcgtt tggcgaaaaa ccgtgccttt gatgccgtct
    10681 aaataattca tcaacagagt gagtttctga tcgggatcag tgactttatt gagattatca
    10741 tgaaaaagca gaacctcgtt gaaggtcgaa gcaacttcag cgacgaaaat cgagtagtcg
    10801 gaaagcggaa aaggttgggt tttattggaa aagtagctat gcatagtgtg accgagttcg
    10861 tgcgccaggg tcgcgacgtc atcgtattta ttacagtaat tgagcaaaac gtagggatgc
    10921 acgtcgtaaa ttgagccgtc ggaataggcg ccactccgtt tagcaggagt tgggtaaacg
    10981 tcgatccaac gctcgttaaa agcccgctca ataacgctga gatagtcact tcctagcggc
    11041 ttcagagcgc ttaaaatcat ttgctgagca gtttcaatgg gataatcaat atccaaatcc
    11101 ttgacaactg gtgtgtagag gtcggtatat ttcaatgtat ctacaccgag caactgctgt
    11161 ttaattttaa ggtaacgata aaaagtcggt agattgtcgt taacgttttt tatcaaagcc
    11221 gtatagacat cggtaggaat gttatttgca tcgagggcgg tttccaaaca actgttgtaa
    11281 ttacgaacgt gagcgataaa aaggtgctgt ttgacgttgg cgtccatagt tgccccgaag
    11341 gtgcgacgga aatcatacat tttgccaaag aaagagttga aaactaattc gcgatcggct
    11401 cgcactggca gagcgcgcaa ggtagcaaag gtagctagat cgagtttaac tttggtgccg
    11461 tcgcttaaaa cgatttccgg gaaagggaat tcagcattgc taaaaagac
//
LOCUS       DAOOMU010000072        11431 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_6343_length_11431_cov_21.957718, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000072 DAOOMU010000000
VERSION     DAOOMU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11431
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6343_length_11431_cov_21.957718"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1570
                     /locus_tag="PLI55_07520"
     CDS             53..1570
                     /locus_tag="PLI55_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HPB00723.1"
                     /translation="MTNLQAQKCQKYHSDNGDGTFTNPVIPADFPDPDVIRVGDTYYM
                     VTTTMFIFPGVTILKSQDLVNWEYCCNAVSRFDFSPCYNLDGCNRYGHGQWATSMRYH
                     NGTFYLLFITLDEGGFLCSATKAEGPWEIKQLPKGFYDPGLFFDDDGRIYVAHGYGKI
                     YMTELDNNFAPISKDSLVYTGDIRDGLEGTHVYKINGYYYLYCTYGGVDGIQVALRSQ
                     NIYGPYEQKVAIRDTMPGVTYGVHQGALIQTQAGEWWTMLFVDSGPFGRFPSLQPVTW
                     VDGWPIVGVDGKGVVTYRKPDVGKQHPIKTLPTSDEFKDKILGMQWGWNHNPDDSRWS
                     LTQRPGYLRLVTGKVVASLPEARNMLTQRIFACYDQTVPTIGITKMEVGKMKDGDIAG
                     LAVFQNPYAFIAVKQENGIRKLIMVNNGETIAETPMKGETVFLRAIASNRTKNARFEY
                     SYDNIEFIQLGNELLMEFSLKIFTGNKFGLFNYATKESGGYVDFDWFRTPMGLSN"
     gene            complement(1767..3320)
                     /locus_tag="PLI55_07525"
     CDS             complement(1767..3320)
                     /locus_tag="PLI55_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPB00724.1"
                     /translation="MEKTILISETAKETRVAILENGELVEFFVEKIDKNRLVGSIFKG
                     KVENVIEGIQAAFVDIGYESNAFLPFSEIEDSSSLAYIENISRDEEEDEQDIEDDLKP
                     TKKIHLRRGDINLQKDQEILVQVIKEPFAKKGPRVTTNISLPGRFLVLVPHAEYIGIS
                     KKILNPNEKRRLRRIAKSIKPPEFGLIIRTVAAGKDVKSLRADLDSLMEKWKQLEEKV
                     KKSKAPACVYTDMELSSTIIRDLLTPDVDKIIVDTKDLYRNILSYIKSTSPEFVNKLE
                     YYHSKNPLFEQYNIEKEFEKSLNKKVWLKSGGFIVIEHTEAMTTIDVNSGKFIGKNDQ
                     EVNSLKINLQAAKEIARQLRLRDVGGLIAIDFIDMEMEENKRRVVQELKRELQKDRAK
                     VSVANISDYGILEMTRQRTRLNLLQSVSEECPTCHGSGRVTSKGSMVTKIESWFRRFK
                     LKSNERRLDLHVNPDFAHYLKDTTNKILWKIQWRNVLRIKLVEDSNVNIDEFRVYLRK
                     SGRDVTDEY"
     gene            complement(3340..>3639)
                     /locus_tag="PLI55_07530"
     CDS             complement(3340..>3639)
                     /locus_tag="PLI55_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HPB00725.1"
                     /translation="GFQYLVQINECIDCELLRRIEEFLTAREIEFQRETSKSQQKINL
                     RSFVETIRCKEPNLQIVTKVINGRTVKIKEILQTLNIDGTNCRIVRQKTYLTPVE"
     assembly_gap    3640..3739
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3740..>5240)
                     /locus_tag="PLI55_07535"
     CDS             complement(<3740..>5240)
                     /locus_tag="PLI55_07535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB00726.1"
                     /translation="APFFDLFVIGDAEEIVVPLVRLLAKGRRQSTPRQVILEEALHRF
                     AGIYVPTFYEVENGRTIPATSFAPAVIQANKIRTLQNDYYPQAPLMPLIEIAQDRLVI
                     ELMRGCTEGCRFCQAGMIYRPVRERQPADIIRQIDQSLPQTGYDEVSLLSLSTSDYSR
                     ITPLLTYLEPALESQKIGLSYPSLRLDSFGETVARLGTATRRSGLTFAPEAGSERLRR
                     VINKQITEDNLLKAVELACRYGWRLLKLYFMLGLPTETEADLEAIISLVQKVLAVSGK
                     RLELNITLSTFIPKPMTPFQWVAMDEPLAIQRKLDYLKPRLRALKNVKVMARDPRASE
                     LEGALARGDRRLAGVIETAWRAGAGFDGWQEYFDPVKWDEAFRINNLDKRVFTGELET
                     EKALPWEHIDCGVSREYLLDEYRKAVQGNNTSDCRDGCNQCGVCFKGGPGMIFAEEWL
                     PPQTNQLIIKTDSELIPIRYRLHYRKFGNAVFTSHRDILRIMPGILRRAG"
     assembly_gap    5241..5340
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5341..5556)
                     /locus_tag="PLI55_07540"
     CDS             complement(<5341..5556)
                     /locus_tag="PLI55_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPB00727.1"
                     /translation="MRIILTQVEKPGRYVGNELNAIRKDHSHTAATIALAFPDLYDVG
                     MSYYGFQILYHILNRETDIAAERVYVPW"
     gene            complement(5687..6247)
                     /locus_tag="PLI55_07545"
     CDS             complement(5687..6247)
                     /locus_tag="PLI55_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPB00728.1"
                     /translation="MNYPDAYELLCRYTKTESLRKHALAVSAAMRHYARIFNEDEEYW
                     AITGLLHDFDYEMYPDEQNHPYRGNEILAKEGYPEEMRTAIMGHASYTGIKRESLLAK
                     TLFAVDELSGFIIAVALVRPSKSIFEVEPRSVQKKFKDKAFAAKVNREEIKQGITELG
                     VDEAEHIQNVIDALKSAAAELGLAGS"
     gene            complement(6266..6547)
                     /locus_tag="PLI55_07550"
     CDS             complement(6266..6547)
                     /locus_tag="PLI55_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00729.1"
                     /translation="MKKSLPNPEELSLENQRMLWLRVYTDRIIRQLQTMSLDEPGAVS
                     LLEQVKQEVLQRFPDKERAYCLIYERRFKRILQRRGLFLPLTMDNGEIN"
     gene            complement(6544..7347)
                     /locus_tag="PLI55_07555"
     CDS             complement(6544..7347)
                     /locus_tag="PLI55_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPB00730.1"
                     /translation="MRIAIYQFAPIWGEINNNIENISRAIASQAADLWILPELCTTGY
                     VFSNVEELKSYAENVKEGASVKALCALSHQYQTALVFGMAEKSGDQIFNSAVVFDKGT
                     IPAVYRKVHLFYEEKKLFSAGTETPPVIEIQGVKVGVMICFDWFFPEMARTLALQGAQ
                     IIAHPANLVLPYCQAAMLTRSLENRVFTATANRIGSEMLGAKRLTFTGKSQITSPRGE
                     LLGQLKADRPGILVRDIDPTEALDKSLTPLNDIFEDRRPEVYQLGKSSR"
     gene            complement(7402..8106)
                     /locus_tag="PLI55_07560"
     CDS             complement(7402..8106)
                     /locus_tag="PLI55_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00731.1"
                     /translation="MTPLIEYDKKITQLIAAGRYDEALWVAEEELCYAESSYGHEHLE
                     TAKVLNNLGWICDVLNKEQSAEDYYLKALEVKIAVCGNTSHELIPTLENIINFLINHN
                     QLARAYEFLVKLIDLAGLQNKLWRLRKGAYLCQLAELEEQLGFSDKAENLLWESLSFL
                     QANYGFDHPNAGRVFAKLGAFFERQGNLARAEYCYNRSLKILKKHLSSNHPDVKFSRD
                     GLENIYSKLGLKVKIR"
     gene            8275..>8873
                     /locus_tag="PLI55_07565"
     CDS             8275..>8873
                     /locus_tag="PLI55_07565"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPB00732.1"
                     /translation="MTKQHLAKDKITQLLDRSLDYKEESTAFEHWRTCPDCQAKIRTA
                     IDCAPPWLYNLPNHTNLWAIPVSARWQFWIIGSEEVVLALTFSEKAQRRQIELLRKQG
                     LELARSPAPIFLTNTAEMIYDFLETGQPYKVTALNFCLVSTAFARQVLTWTGLVPFGK
                     TTSYGRIAEWIGRPRAARSVGGALHSNPLGLVIPCHRVLG"
     assembly_gap    8874..8973
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8974..10212
                     /locus_tag="PLI55_07570"
     CDS             <8974..10212
                     /locus_tag="PLI55_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00733.1"
                     /translation="NWLFQLGDDSTWSAPSSNDSNWLPIRVPDYWEKQGFEDYDGFAW
                     YRYHFQADSALLRQDSLILYLGKIDAADEAFLNGVCIGRSGAFPPESLSTKNNVCQYV
                     FPAHLLKPDNLLAVRVYDEGEKGGIYSGPLKIVTVESPKTPLPSGKVLQNSINIIPFT
                     NGIASCCYNLKTRTFTNFQPHIYRKFDEKSETPNLISQAKVVLFRNQEEIPLTNLVTL
                     EVGYLDGTGIVRHILGGDGFKMTIFGYCPFNLNKPFWVFYALLEGEKIDDLSLNFIYA
                     SENLDLDIGKWAYQESNSKWLTVYIFYNPDASDAEQLFLRKYKNEHPGWQALIEEIDW
                     WQKWQAQTLLPENIQEDARKVYLQSLAVLKMAQCREPFPAGGQIVASLPPSTQNYCRP
                     RDQAFAVEALLLSGHSEEAR"
     gene            10228..11133
                     /locus_tag="PLI55_07575"
     CDS             10228..11133
                     /locus_tag="PLI55_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00734.1"
                     /translation="MNSRCGRYKIYQKKDQNQGIGVDYAVSVYRYLGNGTEESVSDEN
                     GLTIHLDNFGLMIWNIYRYVEVTEDLKFLKYYWPKISRNLADVLPTLIDVSGLVRAEP
                     GPWEKSLPYKHYTYTSACTYRGLLDAARLARQMNDENRAQYYEETAINLRINIEKKLI
                     DSQLKALKGNLEDSDPNLYADASSVEALNWIFNPQDQITRNTLELFKNYLTLANAQRG
                     YCRTRQQLITNSQEWVFGDLRIISAARKATRFEEAANLEAWVTRQALNNFGLLPQYFH
                     IRSADYLGAVPRCGLGAGAYIINFW"
BASE COUNT         2989 a         2442 c         2424 g         3276 t
ORIGIN      
        1 taaaaatttc atcgctcgtg cttttagttt tttgcttgta ataacgatat tattgacgaa
       61 tttacaagcc caaaagtgcc aaaaatatca cagtgataat ggggatggaa cttttactaa
      121 tccagttatc ccggccgatt tccccgatcc tgatgtgatt cgagtaggcg atacctatta
      181 tatggttacg accacaatgt ttatttttcc gggagtaacc attttaaaat cgcaggactt
      241 agtaaattgg gaatattgct gtaatgctgt ttctcgtttt gatttcagcc cttgttataa
      301 tctcgatgga tgtaataggt acggtcatgg tcaatgggca acaagtatgc gatatcataa
      361 tgggactttc tatctacttt ttattacact cgatgaaggc gggtttcttt gttcggcaac
      421 aaaagctgaa ggtccgtggg aaataaagca attgccgaaa ggattttatg atccgggact
      481 atttttcgat gatgacggaa gaatttatgt agcccatggc tatgggaaaa tttatatgac
      541 agagctcgat aataactttg ctcctattag caaagattcc ctggtttata caggagatat
      601 acgagatggt cttgaaggta ctcatgtata taaaattaat ggttattatt atttgtattg
      661 cacctatggc ggtgttgatg gtattcaggt ggcgttacgt tcgcagaata tttacggacc
      721 ttacgaacaa aaggtggcaa ttagagacac gatgcccgga gtaacgtatg gagttcatca
      781 gggagcactt attcaaaccc aagctggcga atggtggact atgctttttg tggatagcgg
      841 tccctttggc cgttttccat ctttacagcc tgtaacctgg gttgatggtt ggcctatagt
      901 tggagttgat gggaaaggcg ttgttaccta tagaaaacct gatgttggca aacagcatcc
      961 gataaaaacg ttgccaacat ctgacgaatt caaggataaa atattaggaa tgcaatgggg
     1021 ttggaatcac aatccagacg acagcaggtg gtcactgacg cagaggccgg gttatttgag
     1081 attagtaact ggcaaagtgg ttgctagttt gcctgaagct cggaatatgc ttactcaacg
     1141 tatatttgct tgctatgatc aaactgtacc aacgattggc attactaaaa tggaagttgg
     1201 aaaaatgaaa gatggagata ttgcggggct ggccgttttt cagaatccct atgcttttat
     1261 tgcagtcaaa caggaaaatg gtattaggaa attgattatg gtaaacaacg gcgaaactat
     1321 tgccgaaact ccaatgaaag gtgagacagt atttttacgc gctatcgcct caaataggac
     1381 aaaaaatgct agatttgaat acagttatga taatatagaa tttatccagc ttggtaacga
     1441 attattaatg gaattcagtc ttaagatatt tactggcaat aagtttggac tttttaatta
     1501 tgcgaccaag gaatctggtg gctatgttga ttttgattgg ttcagaaccc caatgggatt
     1561 gtccaattaa tcgtaaggca agtcatttgt tgccgggctg aatcccgagc gcttatattt
     1621 tctcaacatt aacctcgtct tagcttggag tttattgcct gcctgtacta ttctgctctg
     1681 cgggcgcaag caagtcgcca atatcccttg tgatgacata acattatcta actggttatg
     1741 agtaattgcg aattgtggtt attttttcaa tactcgtctg tcacatcgcg tcccgatttt
     1801 cgcaaataaa cccgaaattc atcaatgttg acattgctat cctcgactaa ttttatacga
     1861 agtacatttc gccattgaat tttccagagg attttattgg tagtatcttt caaataatgc
     1921 gcaaaatcgg gatttacgtg taaatccaag cgtctttcat tagacttgag tttgaagcgc
     1981 cgaaaccagc tctcgatctt agtaaccata gagcctttgg aggtaactcg accagaacca
     2041 tggcaagtgg gacattcttc gctaacggat tggagcaaat ttaatcgagt acgttggcgc
     2101 gtcatttcga gaattccata atcggaaata ttggcgactg ataccttagc tcggtctttc
     2161 tgaagttcgc gctttaattc ctgaactact cgccgtttat tttcttccat ctccatatca
     2221 ataaagtcaa tagcgattaa accaccgacg tcgcgcagac gcaattgtcg ggcaatttct
     2281 tttgccgcct gcaaattgat tttaagcgag ttgacttctt ggtcattttt accgataaat
     2341 ttgccgctgt tgacgtcaat agtcgtcatc gcttcagtgt gttcaatgac aataaaacca
     2401 ccgcttttta gccagacctt tttatttaag gatttttcga actccttttc aatattatac
     2461 tgctcgaaaa gaggattctt agagtggtaa tattccaatt tattgacgaa ttcgggagag
     2521 gtgctcttga tataagacaa gatattgcga tacaggtctt tagtatctac gatgatttta
     2581 tcaacatccg gagtcagtag atcgcgaatt atcgtcgaag ataattccat atcggtataa
     2641 acgcaagctg gtgctttgct tttttttact ttttcttcaa gttgcttcca tttttccatc
     2701 aatgaatcga ggtcagcgcg caaagactta acgtccttgc ccgcggcaac cgtgcgaatt
     2761 attaagccga attcaggtgg tttaatagac ttggcaatcc gtcgtaggcg acgtttttca
     2821 ttgggattaa ggattttttt tgaaatgccg atatattcgg catggggaac cagtaccagg
     2881 aaacgccctg gtagagaaat attggtcgta acgcgcggcc cttttttagc aaaaggttcc
     2941 ttaatgactt gtaccaaaat ttcctgatct ttttgtaagt tgatgtcgcc cctgcgcagg
     3001 tgaattttct tcgtcggttt caaatcatct tcgatatcct gctcatcctc ttcttcgtct
     3061 cggctgatgt tttcaatata tgccagcgaa gacgagtcct cgatctcgga aaacggtagg
     3121 aatgcgttgg attcatagcc gatatcaacg aatgcggctt ggatcccttc gattacgttt
     3181 tctactttgc ccttaaaaat tgagccaact aggcgatttt tatctatttt ttcaacgaaa
     3241 aattcgacca gttcgccgtt ttctaaaatt gcgactcggg tttctttagc cgtttcgctg
     3301 attaaaattg ttttttccat ttactttatc ctaaaatttt tattcgacag gcgtaagata
     3361 ggttttttgc cgaacgatcc ggcagttagt tccatcaatg tttaacgtct gtaaaatttc
     3421 tttaatcttt accgtgcgtc cgttgattac tttagtgact atctgaaggt taggttcttt
     3481 acaccggatg gtttcaacga acgaacgcag gttgatcttt tgctgacttt tgcttgtttc
     3541 gcgctggaac tcgatttccc gtgccgtcag gaattcttct attctccgta atagctcaca
     3601 atcaatacat tcattgatct ggactaaata ctgaaaaccn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnc cggcgcgtcg gagaataccc ggcattatac gcaaaatgtc
     3781 ccgatgggaa gtgaacacag cattaccaaa tttacgataa tgtaagcggt atcgtatcgg
     3841 aattaattca gaatcggttt ttataataag ctgattggtc tgaggtggca gccattcttc
     3901 ggcaaaaatc attccaggac cgcctttaaa acaaacgccg cattgattac aaccatcgcg
     3961 acagtcggaa gtgttgttcc cctgcacagc tttccggtat tcatccaata aatattcacg
     4021 acttacaccg cagtcaatat gttcccatgg caatgctttt tcggtttcca attctccggt
     4081 aaacactcgc ttatcgaggt tattaatgcg gaaagcttcg tcccatttta ctggatcaaa
     4141 atattcctgc cagccgtcaa atccagctcc cgcgcgccag gctgtttcaa tgacgccggc
     4201 gagacggcga tctccacgcg ccaatgcgcc ttccaattcc gatgcccgcg gatcgcgcgc
     4261 cattaccttg acatttttca gggcgcgtaa acgtggtttt aaataatcaa gcttacgctg
     4321 aattgcgagt ggttcatcca tcgctaccca ttggaatggc gtcatcggct taggaataaa
     4381 agtcgaaagc gtgatattca gttccagacg tttaccgcta actgccagaa ccttctgaac
     4441 gagagaaatt atcgcctcca gatcagcctc agtttcagtt ggcagaccaa gcataaaata
     4501 gagtttcagc aagcgccaac cataccggca agccaattcc actgctttta gtaaattatc
     4561 ttcagttatc tgtttgttga ttacgcgacg taatcgttcc gaaccggctt ccggcgcaaa
     4621 ggttaatccc gagcggcgag tagctgtccc caatctggca acggtttccc cgaaactgtc
     4681 gagtcgtagt gacggatagg aaagcccgat tttctgagat tccaaggctg gctccaaata
     4741 agttaagagc ggcgtaatgc gactataatc ggaggtggat agcgacagaa gtgatacttc
     4801 atcgtagccg gtttgaggca aagattggtc aatttgccgg ataatgtccg ccggttggcg
     4861 ttcgcgcacc ggacgataaa tcattccagc ctggcaaaac ctacaaccct cagtgcagcc
     4921 ccgcatcaat tcaattacta accgatcctg agcaatttca atcaatggca tcaacggagc
     4981 ttgtgggtaa tagtcattct gcaaagtccg aattttattc gcctggataa ctgccggtgc
     5041 aaaggatgtc gccggaatag ttcgtccatt ctcaacttcg taaaacgtcg gaacgtagat
     5101 tcctgcaaaa cggtggagag cttcttccaa tatcacctga cggggtgtac tttggcgcct
     5161 tccttttgcc agcaaacgca ccaacggcac tacaatttcc tccgcatcac caattacaaa
     5221 taggtcaaaa aagggcgcca nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 ccagggcaca taaactcgtt ccgcggctat atcggtttcc ctatttaaaa tatgatatag
     5401 aatctggaag ccgtaatacg acattccgac gtcatagaga tcgggaaatg ccagcgcaat
     5461 tgtcgccgcc gtgtggctgt gatctttgcg aatagcattc agttcattgc cgacatagcg
     5521 gccgggtttt tcgacctgcg ttagaataat tctcatcagg tcgttcgaat tagtaatggt
     5581 tttcaatttt caatacaata ataacaattc tgaatggcag ataaacaata gaacttcaac
     5641 aaccgataaa ttttagagct atcgagtcag ctaagaattt catatctcaa cttccagcta
     5701 atcccaattc ggcggcggca cttttcaaag cgtcaatgac attttgaatg tgttcagctt
     5761 cgtccacgcc gagttcagtg atgccctgtt taatttcctc gcgattgact ttggcggcga
     5821 aagctttgtc tttgaatttt ttctgcaccg aacgcggctc tacctcgaaa atgctcttag
     5881 aaggacgcac cagagcaaca gctataatga acccgcttaa ttcatctacc gcgaataagg
     5941 ttttggctaa cagagattca cgtttgatgc cggtgtaaga agcatgcccc atgattgccg
     6001 ttcgcatctc ttctgggtaa ccttccttgg ctaaaatttc attaccgcga taaggatgat
     6061 tttgttcatc aggatacatc tcataatcga aatcgtgtaa taagccggta attgcccagt
     6121 attcttcatc ttcattaaat attctggcgt aatggcgcat agcggctgag acggctaggg
     6181 catgtttgcg caaactctca gtcttggtgt agcggcagag caattcatag gcatctgggt
     6241 agttcatttt ctctcctgag caaacttagt ttatttctcc gttatccatc gttagtggta
     6301 aaaacaatcc gcgcctttgc aaaatccttt tgaaacgacg ctcgtaaatt aagcaatagg
     6361 ctctctcttt atcgggaaaa cgctggagga cttcctgctt gacctgctcg agtaggctta
     6421 ctgcccccgg ttcatctaac gacatagttt gaagctggcg tattatccgg tcagtatata
     6481 ctctcagcca gagcattcgt tgattttcta ggctcaattc ttccggattt ggtaaagatt
     6541 tcttcatcgc gacgattttc ccagttgata aacttcgggg cgccgatcct cgaaaatatc
     6601 attgagaggc gtcaatgatt tgtccaatgc ctcagtcgga tcaatgtcgc gcactaaaat
     6661 gccggggcga tcggctttaa gttgtcccaa cagttcacca cgcggactgg ttatctggct
     6721 ttttccggta aaagttagcc tttttgcgcc gagcatttca ctgccgatcc ggttagcagt
     6781 cgcggtaaaa acgcgatttt ccagactgcg ggttagcatc gccgcctggc aatagggcaa
     6841 caccagattg gctggatgag caatgatttg tgcgccctgt agagccagag tgcgcgccat
     6901 ttcggggaaa aaccagtcaa aacaaatcat cacaccaact ttaacacctt gaatttcaat
     6961 aaccgggggt gtttctgtcc cagcagaaaa caattttttt tcttcataaa aaaggtgcac
     7021 tttccgataa accgccggaa ttgtaccctt atcgaacacc acggcgctat tgaaaatttg
     7081 atcgccgctt ttttcagcca ttccgaaaac cagcgcagtc tgatattggt ggcttaatgc
     7141 gcagagcgct ttaaccgagg cgccttcttt gacattttcc gcataagact ttaattcttc
     7201 cacattacta aagacataac cggtggtaca tagttccggt aaaatccaga gatctgccgc
     7261 ttggctggca atagcacggc taatattttc tatattatta ttaatctcgc cccagattgg
     7321 cgcgaactga taaatggcaa ttctcataat aataaaaatg tagtagcagg gaacaaaatg
     7381 atcatttgga aaaagtgtga atcagcgaat tttgacttta aggcctaatt tcgagtaaat
     7441 attttccaaa ccatcacggc taaacttgac atctggatga ttggaagaca aatgtttctt
     7501 cagtattttt aaggagcggt tgtaacaata ttcagcgcgc gctaaattgc cctgtcgttc
     7561 aaaaaaagcg ccaagtttag caaaaacgcg accggcgttg ggatggtcga agccataatt
     7621 tgcctgcaaa aacgaaagac tttcccagag taaattttca gccttgtcag agaagccgag
     7681 ttgttcctcc aattctgcca gctggcaaag ataggcacct ttgcgtaaac gccagagctt
     7741 gttttgcaat ccagccaagt ctatcaattt tacgaggaat tcatacgccc gagctaattg
     7801 attatgatta attaaaaaat taatgatgtt ttccagagta ggaatcaatt cgtgcgaagt
     7861 attgccgcag acggcgattt tgacttccag agccttaaga taatagtctt cggcagattg
     7921 ttctttattg agaacatcac aaatccatcc caggttatta agaactttag ccgtttcaag
     7981 atgttcatga ccgtaactgc tctcggcata acacaattct tcttccgcaa cccagagagc
     8041 ttcatcatac cgtccagcgg ctatcaattg ggtaattttt ttatcgtact cgattagcgg
     8101 tgtcataaaa aagcctcttg ggcgcggtct ttacttattg aacgttctac cacaaggcaa
     8161 atttaatatt accactaata ataactatca atatttttcg gtcgagtgga caatttgact
     8221 tgttgaatta aatagtccta ttaaattaac ctatgttgat ttatttaaag aaaaatgact
     8281 aaacaacatc tcgcgaagga caaaattact caattgcttg atcgtagcct tgattacaag
     8341 gaagaatcaa cggcgtttga acattggcgg acctgtccgg actgccaggc taaaatacga
     8401 acagcaattg attgcgcccc accttggcta tataatctcc ccaatcatac taatctctgg
     8461 gcgattccag tttcagcccg ctggcaattc tggataatcg gctcggaaga agttgtgcta
     8521 gctttaacgt tttcagaaaa agcgcagcgc cgacaaattg aattactgag aaagcaaggc
     8581 ttggaacttg cgcgttcgcc tgcgccgatt ttcctgacca atacggctga aatgatctat
     8641 gattttttgg aaaccggtca gccttataaa gttacggctc tcaatttctg tctcgtttcc
     8701 acagcttttg ctcgccaggt tttgacttgg accggcttgg tgccgttcgg caaaactacc
     8761 agttacggcc ggatagccga atggatcggt cgcccacgcg ccgcccgaag tgtcggcggc
     8821 gccttgcatt ccaatccgct aggattagtt attccctgtc atcgagtcct cggnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaattggc tttttcagct gggcgacgac
     9001 tcgacctggt cagctcccag ttcgaacgat tcgaactggt tgcctattcg cgttccggat
     9061 tactgggaaa agcaaggctt tgaagattat gacggctttg cttggtatcg ttatcacttt
     9121 caggctgatt cggctttatt gcggcaggat tcgctgattc tttatctggg aaaaattgat
     9181 gccgccgacg aagcttttct caatggcgtc tgcatcggac gtagcggagc cttcccgccg
     9241 gaatcgcttt ctactaaaaa taacgtctgc caatatgtat ttcctgccca tttattaaag
     9301 cctgataact tgctggcggt tcgtgtttac gatgaaggtg aaaagggtgg aatttatagc
     9361 ggtccgctga aaatcgtgac ggtcgaaagt cccaaaactc ccttaccgtc aggaaaggta
     9421 cttcaaaatt cgattaatat tattcctttc accaacggga tagcatcttg ttgttataat
     9481 ttgaaaacgc ggacattcac caattttcag cctcatattt atcgcaaatt cgatgaaaaa
     9541 agtgagacgc cgaacctgat tagccaggcg aaagtcgtcc tctttcggaa tcaggaggaa
     9601 ataccgctaa ctaatttagt gacgctcgag gtcggctacc tggatggcac tggaatcgtt
     9661 cggcatattc tgggtggtga tggttttaag atgactattt ttggttattg tcctttcaat
     9721 ttaaataagc ccttctgggt gttctatgct cttttagaag gtgaaaagat tgatgatctc
     9781 agtcttaatt ttatctacgc ttctgaaaat ctcgatctag acattggcaa gtgggcttat
     9841 caggagagta acagcaaatg gctgaccgtt tatattttct ataatcccga tgccagcgat
     9901 gccgagcaac ttttcttgag gaaatataag aacgaacatc ccggctggca ggctttgatc
     9961 gaagaaattg actggtggca aaagtggcaa gcccagactt tattgcccga aaatattcaa
    10021 gaggatgctc ggaaggttta tttgcaatcg ctagcggtat taaaaatggc tcaatgccgt
    10081 gagcccttcc cagcaggcgg tcaaattgtt gccagtctgc cgccctctac tcagaattac
    10141 tgtcggccga gggatcaagc cttcgcagtc gaagcccttt tgctatccgg acatagtgag
    10201 gaagcccgct aagccctcca gtttatcatg aacagtcgtt gtggacgtta taaaatttac
    10261 cagaagaaag accaaaacca ggggattgga gtagattatg ccgtctcagt ttatcgctat
    10321 ctaggcaatg gcacggagga gagcgttagc gatgaaaatg gactgactat tcatcttgat
    10381 aatttcggac tgatgatctg gaatatatat cgttatgttg aggttacgga agacctcaaa
    10441 tttctgaaat attactggcc gaaaatctcg cgcaacctgg ctgatgtttt gccgacttta
    10501 attgacgtta gcggtctggt gcgcgccgag ccgggtccct gggaaaaatc tctgccttac
    10561 aagcattata cttatacctc agcctgcacc tatcgcggct tgttggatgc cgcccgtctg
    10621 gcgcgtcaaa tgaatgacga aaatcgcgcc caatattacg aagagacagc cattaacctg
    10681 cgaattaata ttgaaaaaaa gttgatcgat tcccaattaa aagctttaaa gggtaatctc
    10741 gaagattccg atcccaactt gtatgctgac gcttcgagtg tcgaagcatt gaactggatt
    10801 ttcaatcccc aggatcaaat tacacgaaac acactggaac tttttaagaa ttacctaact
    10861 ctggcaaatg cccagcgagg ctattgccga accagacaac agttaattac aaatagtcaa
    10921 gaatgggttt ttggcgattt gcggattatt tcagccgctc ggaaggctac tcgtttcgag
    10981 gaagccgcta acctggaagc ctgggttact cgccaggctt tgaataactt cggattgctt
    11041 ccgcaatatt ttcatattcg cagtgccgac tatctcggcg ccgtacctcg ctgtggtctt
    11101 ggtgccggag catatatcat taatttctgg tgaaaatgat caactaccga cttggatcca
    11161 gaataatatc cgtaattttt ttattaatat tttaccggac ttgttcgaag catcctttta
    11221 cgccggagat tcgggagatt ctcgttcacc aaaaccgacg cactctggaa aaggtagccg
    11281 atcaatactt gaattcgccc tcagctgaaa ctcgttatcg gctttgtttg gctattgcta
    11341 attgtcagga tacattgctg gttactcagc tcactacttt gttagatgat cagtcgccaa
    11401 cagtgcggac tggagcggct tttgcccttg g
//
LOCUS       DAOOMU010000073        11326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_6428_length_11326_cov_23.364741, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000073 DAOOMU010000000
VERSION     DAOOMU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11326
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6428_length_11326_cov_23.364741"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..2786
                     /locus_tag="PLI55_07580"
     CDS             123..2786
                     /locus_tag="PLI55_07580"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPB00735.1"
                     /translation="MSIQLDKVYDPRQVETKWYDYWLSKNYFHAKADSPKKPFVIVIP
                     PPNVTGILTIGHVLNNTMQDILIRHARMQGFETLWLPGTDHAGIATQNVVEKNLRKAG
                     KTRYDYSREEFTKLVWDWALKHKDIILTQLRRIGCSLDWERERFTLDEGLSRAVRQVF
                     VELYHKGLIYRGRRIINWCPASQTALSDEEVVHRDTKGHLWYFRYPLADNSGFITVAT
                     TRPETMLGDTAVAVNPADSRYQKLVGRNVKLPIIGRIIPIIADEFVDREFGTGAVKVT
                     PAHDPNDFEIAERHNLPKINILNPDATMNEAAGPLVAGKDRFEARQLVVAEMERLGLL
                     EKIEEYSHSVGYSERAGVMIEPYLSEQWFVRMQPLAEPALKVVEEKEIRFYPEKWVKV
                     YKHWMTNIRDWCISRQLWWGHRIPVFYCDACGWSDALMEDPSACPKCGGAVRQDEDVL
                     DTWFSSWLWPFSTLGWPDDTPDLRKFYPTDDLVTGPDIIFFWVARMIMAGLEFCHEIP
                     FRNVYFTGIIRDAQRRKMSKSLGNSPDPLVLVEKYGADALRFGIMLIAPQGQDILFSE
                     ERLEVGRNFMNKVWNAARFVLMNLDENTLSGEPFEPKRMQLELADEWILSRLNDTIRS
                     VDKNLKKYRFDTFARTIYDFVWSDYCDWYIELIKDRLYKKSAAEKQTALQVAVYVLKN
                     FLKLLHPYAPFITEEIYQHIKLPNEPDIVVSTWPKVEYHPKDKALTERFELLQEIVTA
                     LRTARSEMNISPAVSIALIVRSARSSPVTELLNNSEIQNYIKNLVKVTQITFVAENVK
                     PAHSSTVVVRGTEFFIPLEGVIDIAAETARLTKEINRLTGILKGASAKLSNRDFLARA
                     PQEIINKERAKEQVLKEQLDKFKANLKLLNN"
     gene            2832..4211
                     /locus_tag="PLI55_07585"
     CDS             2832..4211
                     /locus_tag="PLI55_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00736.1"
                     /translation="MKKLKNGILLATIILGLIRMIFADTPVKSTQITVYNNNLGLVLQ
                     TRTLNIAKGDSEIRIEGVPAKVDPTSVRITFPGKADAIEVLEQNFLYDLVNSIKIFEK
                     YIGESISYKTKSGEQTNGKLLQFDGGNLILQLSNGGIKIASAENISEYDFPSLPSGLI
                     LKPTLQWLLSSRISAAVESEISYLTAGMNWHAEYLMVLDKNDRDFTLNSWVSLDNQSG
                     ATYENAKMKLVAGDIHRAPQPAPVRREKVYAADVLTQVAPFEERGLFEYHLYELQRPV
                     TIKDREIKQITLFDEVNAKAEKFYLFKNSAFSETEGPLEVHLKFANSTANHLGIPLPE
                     GVFRIFKKDVDNTLQLVGEDRIAHTAKDDTLDLIVGKAFDVKGQKTITDRQKISDRSE
                     IISIKLEISNKRTEPINVEIQEIMSGDWFVKNASHPYQKKSNSLLVFPLTVPADKNII
                     IKYSFQRSW"
     gene            4205..>4647
                     /locus_tag="PLI55_07590"
     CDS             4205..>4647
                     /locus_tag="PLI55_07590"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPB00737.1"
                     /translation="MVNERPLDKRNIELRPETPITYVKGVGPKRAEVLAKVGINTLKD
                     LLYYFPRRYLDRSTITEIAQLKVGMDATVVGKIISQNLVRRYRRPFFQVEISDGSGIM
                     TAVWFNGINYIKNAFKVGETVAMSGKVEFFKGLRFIHPDYDKLET"
     assembly_gap    4648..4747
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4748..6166
                     /locus_tag="PLI55_07595"
     CDS             <4748..6166
                     /locus_tag="PLI55_07595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPB00738.1"
                     /translation="FEELFFLQLLLAVRRAGIKAIQKPQRYKSAGPLLKQIYAKLPFE
                     LTNAQKKVVREIWNDMHSPTLMNRLLQGDVGSGKTVVALLCASIAIGNGFQAAVMAPT
                     EILADQHYRVLKQLGEVAGIQVRLLIGGQKKQERQTILDEIQAGQAELIVGTHALIQE
                     NVSFKNLALVIIDEQHRFGVVQRSTLISKGYHPDVLVMTATPIPRTLSMTLYGDMDIS
                     VLDEMPAGKGRIITRAVTADQIGKVYDYIAKQLPKGNQAYVVYPLIEESAKTDLQAAI
                     QGYEYLSEKVFPQFRLALLHGGMSIAEKDRIMQDFIQGKIDLLVSTTVIEVGVDNPNA
                     TIMLVENAERFGLTQMHQLRGRIGRGRKNGVCILVERKVTELGHQRIKTILSTIDGFE
                     ISEADLKLRGPGEFYGTRQHGYLKMKIADPLTDIELLKTARVRAFTLLKSDPHLRRSE
                     NTATRNHLLQQYANQIDFSNIL"
     gene            6175..6486
                     /locus_tag="PLI55_07600"
     CDS             6175..6486
                     /locus_tag="PLI55_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HPB00739.1"
                     /translation="MPEKQPDRQQILFLSLVQSLVSSAWIHLGKMKNPVTGTNSIELE
                     EASMSIDMLEMLHNRTQGNLSDDEKRFLENALSDLKINFVEAKLHQSAASEKPNEESH
                     S"
     gene            6513..7505
                     /locus_tag="PLI55_07605"
     CDS             6513..7505
                     /locus_tag="PLI55_07605"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPB00740.1"
                     /translation="MNWFKKDIEKKILALLTLLLSFIVYLDTISPTVPFWDCGEFIAT
                     AYTLGVPHPPGSPLFLLIGRIFSMIPFSQDIAFRVNLISTIVSALAVMFLFLITVQLL
                     QFRGKPTTKLQKLIVYGGAFIGAMTFAFTDSHWFNAVEAEVYAFSTFLTAIVVWLILV
                     WEEKAGQPGHERYLLIIAYIVGLAIGVHLLNLLAIFFIALIIYFKQKKVSSVWWLILD
                     IIIGVAVAGLLFLISMQFKASIFVQAIITLGILVLGYYQLYRKARKPRLKEHAQNVLM
                     AFAASAAFLIIYVGIMKGLPILADKVGLWATVLAIVLLFAVTIWELEKNVISWR"
     gene            7508..9412
                     /locus_tag="PLI55_07610"
     CDS             7508..9412
                     /locus_tag="PLI55_07610"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00741.1"
                     /translation="MMALVLIVVGYSTYVTIFIRSAQDPNIDENDPETTHQAVAYLER
                     EQYGQRSFTDIFNRAQWKPEAAHKYSSALDYFWNYQIKHMYIRYFNWQFIGRKGSDVD
                     FFQFILPFPFIIGLFGLFVHFNEDKHKAVAVLALFLFTGLMIVLYLNQDDPQPRERDY
                     SYVGSFFAFSIWIGIGAAALLERISQLKNKKLSRPLTYITAGLLLLALPINVLVANYH
                     EHDRSGNYVASDYAYNILQSCGPNGILFTNGDNDTFPLWYMQEVEHLRRDVKVVNLSL
                     LNTPWYIKQLRNIEPKINIGNISDATIDNIGPIPWKKQKVRISPPPESLLTSDKYPEF
                     RQFGYNPKEFPPMEWEVEPTLKYGNQGYLRVQDIMVLQILEANRWERPLYFASTVSPD
                     NKLGMEDFMSMEGLTFRIYPQKVKRIDADKMRHNLFNVFRYRNLNNPKVYYDDNIKRL
                     MFNYQTAFIQLAIEELYSGNKERMLATLDTMKYLIPEEIFPIEYDDIYLQIGLLYYDG
                     GRRGEPGQPSELEKRLETLLNKPNVNYRNKLKYASIYAQVLGDYPKAIQILEELNAQK
                     PNDPEISGYLVKVYEDAQQLDKAVRVLDNWLALYPNDSGAAEMRNLYLSKMTPAQKAQ
                     YDSLTKVYHR"
     gene            9424..10464
                     /locus_tag="PLI55_07615"
     CDS             9424..10464
                     /locus_tag="PLI55_07615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPB00742.1"
                     /translation="MSSTPEIAVIIPHYNGVAILRDCLNSLFSSTDLPLDVILVDNAS
                     TDESVKMVRSEFPAVRILPQSQNRGFAGGCNAGIRATEAPFVLILNNDTIHQAGWLEL
                     LLEKIKSQPELVAVQPKILSWQNREQFDYSGAAGGEMDIFGFPFARGRIFETIEMDEG
                     QYDYLPDRIFWASGTAFLARRELLIEAGLFDETFFAHMEEIDLQWRLQLMGYSIGVEP
                     RAVIWHRSGSTLGAEAPLKKYLNHRNSLLMFLANYKPLVTAYLLPIRILLDWMALAFS
                     LLKLDLGRAGAILKAHGWMISHPAVIFKRRRQVKALRRITDFQIMEKLYRGSIALSYY
                     LLKKKSYLDLVD"
     gene            complement(10468..>11326)
                     /locus_tag="PLI55_07620"
     CDS             complement(10468..>11326)
                     /locus_tag="PLI55_07620"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPB00743.1"
                     /translation="EILMKKCKPIQLAMVLLLFLSQIFAQFVDVKVEISVDRMPELER
                     SDLKVLEMAIPAYYENYEWIENTYGIQIPLKINIYPQSVNTTGSERIFTAQLFIVNES
                     GDMRFFEKNFRFVYNTNDPIVHSEMIQTLTGALDFYAWMMIASEMDTYEQLGGNAYYE
                     KARDVATRGQMSERPAGWKDRLQELDEILRLRNYRLMKYHFWAAMDYADQGNREAVAP
                     EIGKALEAIEAIFAENARERYSHIFLDAHARDIMTIIKEFGTIEQAHKLMALDPDNKP
                     VYEKIIYSD"
BASE COUNT         3062 a         2562 c         2530 g         3072 t
ORIGIN      
        1 agtaaaattt ctcgatgaaa aatttgatta tgagttattg tgaattattt taatttcatt
       61 aagttcgttg ttaagccgat tcaagaaaat aggcttatga ttttcagtaa ttagaggttt
      121 tagtgtctat tcaactcgat aaagtttacg atccgcgcca agttgaaacc aaatggtacg
      181 actactggct ctcgaaaaat tatttccacg ccaaagccga ttcacctaaa aagcctttcg
      241 taatcgtcat tccgccgccg aatgttaccg gcatactgac gattgggcac gtgttgaaca
      301 atactatgca ggatattttg atccggcacg cccgtatgca gggttttgaa actctgtggt
      361 tgccaggcac tgaccatgcc ggtattgcca ctcagaacgt agttgagaaa aaccttcgca
      421 aagcaggtaa gactcgctac gattatagtc gcgaagaatt caccaaactc gtctgggact
      481 gggcgcttaa gcataaggat attatcctga ctcagttacg cagaatcggc tgttcgctgg
      541 attgggagcg ggagcgcttt actctcgatg aagggctcag tcgggcggtg cgtcaggttt
      601 tcgtcgagct ttatcataaa ggtttaatct atcggggtcg gcggattatc aactggtgtc
      661 cggcttcgca gacggcgctt tccgatgaag aggtggttca ccgcgatacc aaaggtcatc
      721 tctggtattt ccgctaccca ttagcggata attccggctt tatcaccgtt gctactactc
      781 gcccggaaac tatgttgggc gacacagccg tggcggtcaa tcccgctgat tctcgttacc
      841 aaaaattagt cggcagaaat gtcaaactgc cgattatcgg gcgaataata ccgattattg
      901 ccgacgaatt cgttgatcga gaattcggca ccggcgccgt caaggtaaca ccggctcatg
      961 atccaaatga tttcgaaata gccgagcgcc acaatttgcc caaaattaat atcctaaatc
     1021 ctgacgctac gatgaatgaa gccgccggac cgctggttgc cggtaaggat cgtttcgaag
     1081 ctcgtcaact ggttgtagct gaaatggaac gcctcggttt gctggaaaaa atcgaggagt
     1141 actcgcattc cgtcggatac agcgagcggg ctggagttat gatcgagcct tatctttccg
     1201 agcagtggtt tgtgcggatg caaccgctgg cagaaccggc gctaaaggtc gtcgaggaaa
     1261 aggaaattcg tttttatcct gaaaaatggg tcaaggttta taaacattgg atgaccaata
     1321 tccgcgactg gtgcatctca cgccaactct ggtggggtca tcgcattcca gttttttact
     1381 gcgacgcttg tggctggagc gatgctctga tggaagaccc gtccgcttgt cctaaatgcg
     1441 gcggcgccgt gcgtcaagat gaggatgtac tcgacacttg gttcagttcc tggttgtggc
     1501 cattctcgac gttaggctgg ccggatgata cccccgattt gcgcaaattt tacccaaccg
     1561 atgatctcgt cactggtccg gacatcattt tcttctgggt agcgcgaatg ataatggctg
     1621 gactcgagtt ctgtcatgaa attccttttc gcaatgtcta tttcaccggc ataattcgcg
     1681 acgctcagcg ccgcaaaatg agtaaatcgc tcggcaattc tcccgatccg ctggttctag
     1741 tcgagaaata tggcgcggac gctctgcgtt ttggcattat gctaattgcc ccgcaaggtc
     1801 aagatatttt attcagtgag gaacggctgg aagtcggacg caattttatg aataaggtct
     1861 ggaacgccgc ccgctttgtc ttgatgaatc tggatgaaaa tacgctaagc ggtgaacctt
     1921 tcgagccgaa gcgtatgcaa ctggaattgg cagatgagtg gattctaagc cgcttgaatg
     1981 atactattcg ctccgttgac aaaaatctta aaaagtaccg tttcgatacc tttgcgcgca
     2041 cgatctatga tttcgtctgg agcgactact gcgactggta tattgaactc atcaaggatc
     2101 ggttatacaa aaaatcagcg gcagaaaaac aaactgcctt gcaagtggcg gtctatgttc
     2161 taaaaaattt cctcaaactg cttcacccct atgcaccctt cattactgag gaaatctacc
     2221 agcatatcaa attgcctaac gagccggaca tcgtcgtctc aacttggcca aaagtcgaat
     2281 atcaccccaa ggataaagcc ctgactgagc gtttcgaact cctgcaggaa atcgtcaccg
     2341 ctctgcgcac cgcccgcagt gaaatgaaca tttcaccggc tgtgtcgatt gcattgatag
     2401 ttcggtctgc gcgcagttcg ccggtcaccg aactactgaa caattccgaa attcaaaact
     2461 acattaaaaa tctggttaaa gttactcaaa taacgttcgt tgccgaaaac gtcaaaccgg
     2521 ctcattcttc aacggtcgtt gtccgcggca ccgagttttt cattccattg gaaggtgtaa
     2581 ttgatattgc cgccgaaact gcgcgcctca cgaaggaaat caaccgttta accggaatat
     2641 taaaaggcgc ttctgccaaa ttatccaacc gcgacttcct cgcccgtgcg ccgcaggaaa
     2701 ttataaacaa agaacgcgca aaagagcaag ttctgaagga acaattagat aaattcaaag
     2761 cgaaccttaa gttgctgaat aactaagtca cgttcgctga ttatttttta aatcaaattg
     2821 aggagagtcg tatgaaaaag ttaaaaaatg gaatactttt agcgacgatc attttgggac
     2881 ttattaggat gattttcgct gatacgcccg tcaagtccac tcaaattacc gtttataata
     2941 acaaccttgg attggtactc cagactcgca cgctcaatat cgccaaaggc gattcggaaa
     3001 ttcgcattga aggcgttccg gctaaagttg atccgacctc ggtgcgcatt acatttcctg
     3061 ggaaagccga cgctatcgaa gtcctcgagc agaattttct ctacgatctg gtcaattcta
     3121 tcaaaatatt cgaaaaatat atcggcgagt caatctcata taagaccaaa tctggcgaac
     3181 agaccaatgg aaaattactc cagttcgatg gtggtaatct catcctacaa ctttcgaatg
     3241 gcggcatcaa gatcgcttcg gctgaaaata tttccgagta cgattttcca tccctgccgt
     3301 ccggattgat tttaaaaccg actttgcaat ggttgctgtc gtccagaatc agtgccgcag
     3361 tcgaatccga aatcagctat ttgactgccg gaatgaactg gcacgccgaa tatttaatgg
     3421 tgctggataa aaatgaccgg gactttacac tcaattcctg ggtgtcgctc gataatcaat
     3481 ccggcgccac ttacgaaaac gccaagatga agctggtcgc cggagatatt catcgcgctc
     3541 cccaaccggc gccggttcgc agagagaaag tctatgccgc cgatgtcctt acgcaagtcg
     3601 caccctttga agaacgcggc ttgttcgagt accatctgta tgagctccag cgcccggtaa
     3661 ccattaaaga tcgggaaata aaacaaatta ccttattcga cgaagttaac gccaaagcgg
     3721 agaaatttta tctattcaaa aattccgctt ttagcgaaac ggagggaccg ctggaagtcc
     3781 atttgaagtt tgccaattca accgccaatc atctcggtat tccgctaccg gagggcgttt
     3841 tccgtatttt caaaaaggat gttgataata ccctgcaatt agtcggcgaa gatcggattg
     3901 cgcacaccgc caaagacgat accctcgact tgatagtcgg caaggctttc gacgtcaaag
     3961 gccaaaaaac aatcaccgat cgccagaaaa tcagcgaccg ttcggaaata atttcgataa
     4021 aattggaaat ttcgaataag cgcacggagc cgatcaacgt cgagattcag gaaataatga
     4081 gcggcgattg gtttgtgaaa aatgcgtcgc atccttacca gaagaaaagc aatagtctgc
     4141 tggtttttcc attgactgtc cccgccgata aaaatatcat catcaaatat tccttccaga
     4201 gaagttggta aacgaacgac cgctcgataa aagaaacata gaactgcggc cagaaacgcc
     4261 gattacctat gtcaaaggcg tcggtccgaa acgcgccgaa gtgctggcga aggtcggcat
     4321 taacacttta aaagatttac tatattattt tccccgccgc tatctcgatc gtagtacaat
     4381 tacggaaatc gctcagttaa aagtcggtat ggacgctacc gtcgtaggaa aaataatttc
     4441 gcagaatttg gttcggcgct atcgccgacc gtttttccag gtcgagatta gcgacggtag
     4501 cggcattatg acagcggtct ggttcaacgg tattaattac atcaaaaatg ctttcaaggt
     4561 cggcgaaaca gtcgcaatga gcggcaaggt ggaatttttc aaaggcttgc gcttcatcca
     4621 tcccgattat gataaactcg aaactgannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnttt gaagaacttt ttttcctgca acttttattg gcggtgcgcc gagccggaat
     4801 caaagccatt caaaagcctc agcgctataa gtctgccgga ccgctattaa aacaaattta
     4861 tgcgaaatta ccgttcgaat tgaccaacgc ccagaaaaaa gtcgttcgtg aaatctggaa
     4921 tgatatgcat tctccgacat tgatgaatcg cctgcttcag ggcgatgtag gcagtggtaa
     4981 aaccgtcgta gccctgctat gcgccagcat tgcaatcggc aatggctttc aagcggcggt
     5041 gatggctccg acagaaattt tagccgacca acattaccgc gtcctgaagc agctcggcga
     5101 agtggctggc atccaagtcc gcctgctaat cggcggtcaa aaaaagcagg aacgccagac
     5161 aattctcgac gaaatccagg ctggacaagc ggaactaatc gtcggaacac acgctctcat
     5221 tcaagaaaat gtgtctttca aaaacctggc actggttatt atagatgaac agcatcgctt
     5281 cggagtcgtt cagcggagca ccttgattag caaaggctac catccggatg tgctggtgat
     5341 gactgcaacg ccgattccac gcacccttag tatgacactt tacggcgata tggacatttc
     5401 cgtattagat gaaatgccgg ctggtaaagg gcgaattatc acccgggctg taacggcgga
     5461 ccaaattgga aaagtttacg attatatcgc caaacaatta ccgaaaggca accaggctta
     5521 tgtcgtctat cctttgattg aagaatcagc caagaccgat ttgcaagccg ccattcaggg
     5581 ctatgaatat ctcagtgaaa aagtattccc gcaatttcga ctggcgcttt tacacggcgg
     5641 gatgtccatc gccgaaaaag accgcattat gcaggatttc attcagggaa aaattgattt
     5701 gctggtaagt acgaccgtta ttgaagtggg agttgacaat cccaacgcca ctattatgct
     5761 agtcgaaaat gccgagcgat tcggtttgac tcagatgcac caattgcgcg gacgaatcgg
     5821 tcgcggtcgc aaaaacggtg tctgtattct ggtcgagcgc aaggttaccg agctgggtca
     5881 tcagcggatt aaaacaattc tctcgacgat tgatggcttt gaaatctctg aggcagacct
     5941 taaattacgt ggacctggag aattttatgg cacacgacag cacggatatt taaaaatgaa
     6001 aattgccgac ccgttgactg acattgagtt attaaaaact gctcgcgtgc gcgcctttac
     6061 gcttttgaaa agcgatcccc atttacgccg cagtgagaat acggctaccc gcaatcattt
     6121 actgcaacaa tatgctaatc aaattgattt cagcaatatt ttataggtga cattatgcct
     6181 gaaaaacaac ctgatcgtca gcaaattctt ttccttagtt tagtgcaatc gctggtcagt
     6241 agtgcctgga ttcatctcgg taaaatgaag aatccggtaa ccggaaccaa tagcatagaa
     6301 ctggaagagg cttctatgag cattgatatg ctggaaatgc tccataacag aactcaaggc
     6361 aatctctccg atgatgaaaa gcgctttctg gaaaacgctt tgagtgactt aaagattaat
     6421 ttcgttgagg ctaaattaca ccagagtgcc gcctcagaga aaccgaatga agaatctcat
     6481 tcataaaaca ataagtctat aaaggaaatt acatgaactg gtttaaaaaa gacatcgaaa
     6541 agaaaattct cgccttgctt acgcttctgc tcagtttcat cgtctatctc gatacaattt
     6601 caccgacggt accattctgg gattgtggcg aatttattgc taccgcctat actctcggag
     6661 ttccgcatcc tccgggtagc ccgctattct tgctgattgg tcgcattttt tcgatgattc
     6721 ctttcagcca ggatattgcg ttccgggtta atttaatatc caccatcgtc agtgcattgg
     6781 cggtgatgtt tctcttttta attactgttc agcttctgca atttcgcggg aaacccacga
     6841 ccaaattaca aaaattgatt gtctatggtg gagcttttat tggcgctatg actttcgctt
     6901 tcactgatag ccactggttc aacgcggtgg aagccgaagt ctatgccttt tcgacgtttt
     6961 taactgctat tgtggtttgg ttgattttgg tttgggaaga aaaagccggt caacccggac
     7021 atgagcgtta tctattaatc atcgcctata ttgtaggtct ggcaatcggt gtccatctct
     7081 taaatctcct ggcaattttc tttatcgcct tgataattta ttttaaacaa aagaaagtgt
     7141 cttcggtctg gtggttgatt ctggatataa ttatcggggt agcggttgcc ggtttacttt
     7201 tccttatttc catgcaattc aaagcgtcaa tatttgttca ggcgataata acattaggta
     7261 ttctggtgct gggttattac cagctttatc ggaaagctcg gaaaccacgc ctgaaagaac
     7321 atgctcaaaa tgtcttgatg gcttttgcgg cttccgccgc ctttctgata atttatgtcg
     7381 gcataatgaa gggtttacca atcctcgccg ataaagttgg actctgggca accgttctgg
     7441 caattgttct cctatttgcg gtaaccatct gggagttaga aaaaaacgta atatcttggc
     7501 gttgataatg atggcgctcg ttcttatcgt tgtcggttat tccacttatg tgaccatttt
     7561 tattcgcagt gcccaggatc cgaatattga cgaaaatgat cccgaaacta ctcatcaggc
     7621 ggtcgcttac ttagagcgtg agcaatatgg ccagcgcagt tttaccgata tattcaaccg
     7681 cgctcaatgg aaaccggaag ccgctcataa atattccagt gctttggact atttctggaa
     7741 ttatcagatt aaacatatgt atatccgcta cttcaactgg caattcatcg gacgtaaggg
     7801 aagcgatgta gatttctttc aatttatctt gccatttccg tttattatcg ggctttttgg
     7861 tctctttgtc catttcaatg aggataagca caaagccgtg gcagtattgg cgcttttcct
     7921 atttacggga ctgatgatag ttttatatct taatcaggat gatccccagc cgcgggagcg
     7981 agactattct tacgtcgggt cattcttcgc attttcgatc tggattggaa ttggagccgc
     8041 ggctttgctt gagcgaattt cccaactgaa aaacaaaaaa ttaagtcgtc cacttaccta
     8101 tataacagcc ggtttattat tattagcctt gccgatcaat gttttagtag ccaattatca
     8161 cgaacatgat cgaagcggta attacgttgc ctcggattac gcttacaata tcctgcaatc
     8221 ctgtggacct aatggtattc tttttactaa cggtgacaac gatactttcc ctctttggta
     8281 tatgcaggag gtcgaacatc tgcgtcgcga cgtcaaagtc gtcaatctta gcctgttgaa
     8341 caccccttgg tacataaaac aattacgcaa catcgaacca aaaatcaata tcggaaatat
     8401 ttccgatgca accattgata atatcggtcc tattccttgg aaaaaacaga aagtaagaat
     8461 cagcccgcca ccggaaagtt tacttacctc agataaatat cctgaatttc gccagttcgg
     8521 ctacaatcct aaggaatttc caccgatgga gtgggaagtc gaaccgactc tgaaatatgg
     8581 taatcagggt tatctgcgcg tacaagatat tatggtttta cagattctgg aagctaatcg
     8641 ctgggaacga ccgctctact ttgcctctac ggtttcacca gataataagc tcggtatgga
     8701 agattttatg tcaatggagg gattgacatt ccgcatttat ccccaaaaag taaaacggat
     8761 cgacgccgat aaaatgcgcc ataacctatt taatgttttt cgctatcgca atttgaataa
     8821 tcccaaggtt tattacgatg ataatatcaa gcgcttaatg ttcaactatc agacggcttt
     8881 tatccaactt gctatcgaag aactttatag cggcaataaa gaacgaatgt tggcgaccct
     8941 cgacactatg aagtatctaa taccagagga aattttcccc attgaatacg atgacattta
     9001 tctccagatt ggtctgcttt attatgatgg cggccgccgt ggtgaaccgg gacaacccag
     9061 cgaactcgaa aaacgcctgg aaaccctatt gaacaaaccc aatgtgaatt accgcaataa
     9121 attgaaatat gctagcattt atgcccaggt gctgggagat tatcctaagg ctattcaaat
     9181 cctggaagaa ttgaatgctc agaaaccaaa cgatccggaa atctccggct atttggtgaa
     9241 agtctatgaa gatgctcaac aattagataa ggcggtgaga gtactggata actggctggc
     9301 actttatcct aacgatagcg gtgccgccga aatgcgtaat ctttatctga gtaagatgac
     9361 cccggctcaa aaggcgcaat acgattcgct gaccaaagtc tatcaccgct aaaccagccc
     9421 ggtatgtcgt ccactccgga aatcgcggtc attatccctc actataacgg tgtggcgatt
     9481 ttgcgagatt gtctgaactc gttgttttcc tcaaccgact tacctctcga tgtcatactg
     9541 gttgataacg cctccaccga cgaaagcgtt aaaatggtta gaagtgaatt cccagccgtc
     9601 cggatccttc cacaatctca aaatcgcggt tttgcgggcg gctgtaatgc cggtattcgc
     9661 gctacggaag ccccatttgt cctaatcctg aataatgata ccattcatca agccgggtgg
     9721 ttggaattat tactggaaaa aatcaaatca cagcccgaat tagtcgccgt tcagcctaaa
     9781 attctttcct ggcaaaaccg tgaacaattt gattattccg gagcggcggg aggcgagatg
     9841 gatattttcg gctttccgtt tgcccgcggt cgaattttcg agactattga aatggacgaa
     9901 gggcaatatg attatttacc tgatcgcatt ttctgggctt ccgggacggc ttttttagcc
     9961 agacgggaat tattaatcga agcgggactt tttgatgaaa ctttcttcgc gcatatggaa
    10021 gaaattgatc tccagtggcg actgcaactt atgggctact ctatcggcgt cgagcctcgc
    10081 gctgtcatct ggcatcgtag tggttctact ctgggagcgg aggcgccgct aaaaaagtat
    10141 ctaaaccatc gtaacagtct gttgatgttc ctggcgaatt ataagccgct ggtgaccgct
    10201 taccttttgc cgattcgtat tctgctggac tggatggcgc tggcattttc cctattgaaa
    10261 ttagatttag gtcgcgccgg tgccattctg aaagctcacg gctggatgat cagccacccg
    10321 gcagtcattt ttaaacgccg ccgccaggta aaggctctcc gtcggattac cgatttccag
    10381 ataatggaaa aattataccg tggttcaatt gccttgagct attatttact gaagaaaaag
    10441 tcttatttgg acttggtaga ttaaaaatta atccgaataa ataatttttt cgtaaactgg
    10501 cttattatcc ggatcaagcg ccatcagttt gtgagcttgt tcgatagttc caaattcctt
    10561 gatgatcgtc attatatcgc gggcatgggc atcgagaaaa atatgcgaat atcgttcgcg
    10621 ggcattttcg gcaaatatcg cctcgatggc ttccaaggct ttgccaattt cgggagcaac
    10681 cgcctctcta tttccttgat cggcatagtc cattgccgcc cagaaatgat atttcattaa
    10741 tcggtagttt cgcaggcgga gaatctcgtc caattcttgc aggcggtctt tccaaccggc
    10801 gggtcgttcg gacatttgcc cgcgggttgc tacgtcccgc gctttctcgt aataggcgtt
    10861 gccgcccagc tgttcgtagg tatccatttc actggcaatc atcatccaag cataaaagtc
    10921 cagcgcaccg gtcagggttt gaatcatttc ggaatggact atcggatcat tagtattata
    10981 gacaaacctg aaatttttct caaaaaaacg catatcgcct gattcattga caatgaacaa
    11041 ctgggcagtg aaaatccttt cggaaccggt cgtattgaca ctttggggat agatattgat
    11101 ttttagtgga atttgaatgc cataagtgtt ttcgatccat tcgtagtttt cgtaataggc
    11161 agggattgcc atttcgagga ctttcagatc cgagcgttcc aattccggca tacgatccac
    11221 cgatatttcg actttgacgt ccacaaattg agcaaatatt tgacttagaa aaagtaacaa
    11281 taccatcgcc agctggatag gtttgcattt tttcattaga atttcc
//
LOCUS       DAOOMU010000074        11320 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_6431_length_11320_cov_17.801065, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000074 DAOOMU010000000
VERSION     DAOOMU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6431_length_11320_cov_17.801065"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..2060
                     /locus_tag="PLI55_07625"
     CDS             129..2060
                     /locus_tag="PLI55_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPB00744.1"
                     /translation="MEDNEKVMTESTVRAPKSTTPSTIKAPEDISAIGATIKVTIDGK
                     EIRVPMGTTILNAARMLGINIPTLCHHNDLCISGVCRICVVEVEGMRTLQASCAYPIS
                     QPIVVHTHTRKVRQARRHVVDLLLARHYGECYSCVRNNNCELQQLAHEYGVDFYRFGH
                     VDKPTYGRDNSSYAIVRDMDKCILCRRCVRTCIDLQEVGVYEAFERGNQTRIGTFMDN
                     PMAELICINCGQCINRCPTGALHANDPSDAIWAAIDDPTKHVVIQTAPAPRSAIGECF
                     GLPAGTPLTFEMNTAMRYCGFDKVFDTNFSADLTIIEEGTELILRLYKALVKKEPVSL
                     PLFTSCSPGWVKYIEHFYPEMLSHLSSAKSPQQMFGAVIKTYYAELNNIDPADIVTVA
                     LMPCTAKKFECNRPEMNSSGFKDIDYGLTTRELAGMFQEAGIDLPNMPKSDFDEPFGT
                     RTGSGVIFGATGGVMESAIRTVYELITGDRIDNLFERARIMPVRGFEGIRYAELPIAK
                     VGPVPDLLKHLIPNWDWLRGATLKVAVAHGTANAKKVLDNIKSGGKLSECHFIEFMAC
                     PGGCLGGGGMPIPTNEEIRAARAQAIYAEDEASEVRKSYENPAVVRLYNEFLVDGPCG
                     HKSHKLLHTYYTPRGKKLV"
     gene            2188..3378
                     /locus_tag="PLI55_07630"
     CDS             2188..3378
                     /locus_tag="PLI55_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPB00745.1"
                     /translation="MPNRFIYLDHNATTPLHPEVKNTITDMFDVFGNPSSLHTVGRIA
                     RQKIEQAREIVANFIGAAPDEIIFVGSGSEANNSVLNSLSCPSQVCRLERNLRDEIIT
                     TSIEHPCVLETSKCLRERGTTVHYLPVDITGKVKLDVLKELLSEKTALVSVMYANNEI
                     GTIQDIKAIAEIAHAQGAFFHTDAVQAVGKIPVNVQDLDVDFLAFSAHKIYGPKGVGA
                     LYVKRGTPFCPLIRGGHQERGRRAGTENTIGIMAFAKAIEMRKQEMSDEAVRLRRFKK
                     MLIDGIKYKIPDVQFNGHPTDTLPGTLNISFAGAEGESILLYLDLEGIGVSTGSACAS
                     GSLEPSHVILATGVGAELAHGSIRISMGRENTTDDIYYTVSALERVVKKVRDMSTTYQ
                     SKLV"
     gene            3375..3995
                     /locus_tag="PLI55_07635"
     CDS             3375..3995
                     /locus_tag="PLI55_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012475599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPB00746.1"
                     /translation="MSNQSGWLYSEIVKDHFMNPRNILRDDENFVADGVGITGNIKCG
                     DEMQFLIQVDKQKGVITDCRWRTYGCASAIASTSVLSEMVKGMKLEDAYRITPKDVVK
                     NLGSLPEHKIHCSVLGDKALRAAIDDYYRRNGMNEKIKHEEARVICQCMNVTDHEIEE
                     AVLEGARTYQELQERTKLGTVCGQCQEEATELLKKYIAKHFGDNRK"
     gene            complement(4243..5325)
                     /locus_tag="PLI55_07640"
     CDS             complement(4243..5325)
                     /locus_tag="PLI55_07640"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPB00747.1"
                     /translation="MNNTSATPTSQLSKTIWRIFLIIMGVAFIFSIFSYVKTVLLMLL
                     IAWLISVILNPLVDYLESIGFNRALAILTIMAVILGGIIALIIGIIPTVIKAVESISA
                     TLKSDLISNLTIQLEQFFQEKFNNPDLARDLINKAEQIGGTLLVKVAGFLKNAGTYVA
                     FVGIVPIITFFLIKDRRLFKRALISQVPNRYFELFLNVLHKVDSQVTKYIQGQAIDAL
                     IVGLLSVIGLFIINLVFDNPVPYFFVIGMIAGVANLIPYLGPYVGAVPAIVLTILNNP
                     NNVGYIIFWIIVVFVLVQLIDNNLVSPMVVSKSVNMHPITVVIAVIIGGNIAGMFGML
                     FAVPFWGIIKVTMREVSWGLRSYKLK"
     gene            complement(5318..5842)
                     /locus_tag="PLI55_07645"
     CDS             complement(5318..5842)
                     /locus_tag="PLI55_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HPB00748.1"
                     /translation="MNHPLWDPISAKNKSEMSDIMQILAKVPIFSDLDSKELKEVEKI
                     IYHRKYKKGEPIFRMGDPGLGMYIIVNGAVEIVEEPENGTRTKLAELEKGAFFGDMAL
                     LDEKPRSASAIAMVDSDIIGFFRPDLVDLLYRKPRMGIKILWALSRVVGERLRKTDEQ
                     LTNLQREMKAKRHE"
     gene            6140..7345
                     /locus_tag="PLI55_07650"
     CDS             6140..7345
                     /locus_tag="PLI55_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPB00749.1"
                     /translation="MLLIRNFLIIIVLLFLSPVQVNSQEKDLDQTIEYQSEQLEKIRK
                     EIRNYKSDLKKAESKEKSILTQIDETERSISLTEKLIAQLNREQIQKEREIEQIERSI
                     KQLESDLNTLKNDFAKRLVYLYKNGKYNDLELILTAKSINQAFYRYKYLRILADIDRR
                     TCTLIRKDINTINQKKQQLNQELRNKEKIINEKKDLMANLQQQKRRHNVQLAKARQNK
                     SNIAAQIKEKEKNAAEISKLIANLDKEREKRNKELERQRALSGVAESNPFLASQGELI
                     WPAKGEIVSRFGIQKHPTLKTITDNPGIDIKVKLGTPVVSVLDGVITTITYIRGFGNT
                     IIIDHGSGFYSVYSHVENIRVAENDYVTVNSVIAEVGQSGSLSGPMLHFEIWRNKTKL
                     NPEEWLNKK"
     gene            7342..8472
                     /locus_tag="PLI55_07655"
     CDS             7342..8472
                     /locus_tag="PLI55_07655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPB00750.1"
                     /translation="MTLFPLINQTENRNLLRNIVATGRVANTYLFYGPEGSGTEGFAL
                     EFAAMLNCSSKSERPCGICQSCKQMKTLEHPNLTLVFPIPGSPDSKPGDSPFKKFKEG
                     EMDEIRQIIQNKSRNPYDKIIVKKALVIPINFIREIKRHIYLAPQNGGQKVVVIFDAH
                     LMNDEAANAFLKILEEPPDNSTIILSTAYPDQLLPTVQSRCQWLYFPPLPEAELKEFL
                     IKKGYPLENIRLAIRLSGGNVSQLTQLIEHDLSDIKELTLAIFLDIAAWKVSNLYNHI
                     QKMTTIYRQDPEKFAQLMQSLIFWIRDAELIRQGQPIDDIIHLDYAERLTNFVKKFPD
                     FDAQNMKVSVENCVDFINKNVYINLALIEMFFKLKKQLNNSK"
     gene            8475..9296
                     /gene="ricT"
                     /locus_tag="PLI55_07660"
     CDS             8475..9296
                     /gene="ricT"
                     /locus_tag="PLI55_07660"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPB00751.1"
                     /translation="MNTSDKLIKIKFKGGNRQLCQNPLDFPVQPNDKMVVSADRGEEL
                     GCALSSGSPKNLPEELGEIPSLLRKATPQDLSQSNENRLQEKMALEFCVAKISELNLP
                     MKLIDVDVRLDRRKIVFNFTADERIDFRELVKLLAAEFKTRIEMRQISSREEMKRTDG
                     VGLCGLRLCCASFIDDFDPISTQFAKEQNLPMNPAKISGCCGKLKCCYRYEHDFYEEV
                     LKNYPAYGTKVKVADKTGILEKIDIFAQTLTIRYDDNTAEDFPLTDFNQKITTVQ"
     gene            9307..11199
                     /gene="metG"
                     /locus_tag="PLI55_07665"
                     /pseudo
     CDS             9307..11199
                     /gene="metG"
                     /locus_tag="PLI55_07665"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
BASE COUNT         3398 a         2566 c         2271 g         3085 t
ORIGIN      
        1 gaaacaattc aactagcttc caaatgcggt ttgggtcaat ccagtccgaa tgcctttgtc
       61 tcaattattg aaaattttaa atcagaaatt ttaggccgtg tgcctgaaaa agcttaaaga
      121 ggatatttat ggaagataac gaaaaagtaa tgactgagtc cacggttcgc gcacccaaaa
      181 gtactacgcc atccaccatt aaagctcccg aagatatcag tgcgattggt gcgactatca
      241 aagtcacaat tgatggaaaa gagattcgcg ttccgatggg gaccacgatt ctgaatgccg
      301 ctcgcatgct cggcatcaat attccaactt tatgtcatca taacgacttg tgtatcagcg
      361 gtgtttgccg tatttgcgta gtagaggtcg aaggtatgcg cactctccag gcttcctgtg
      421 cctatccaat cagccagccg attgtagttc atactcacac ccgcaaagtt cgccaggcgc
      481 gacgtcatgt tgttgacctt ttgcttgcgc gtcattatgg cgaatgttat tcctgcgtcc
      541 gcaataataa ctgcgaatta caacaattag cccacgaata tggtgttgat ttctatcgtt
      601 ttggccatgt tgataaaccg acttatggtc gcgataactc cagctatgca attgtgcgtg
      661 atatggataa atgtattctc tgtcggcgtt gcgtccgcac ctgtattgac cttcaggaag
      721 ttggagttta cgaagccttt gaacgtggca accaaacccg cattggtact ttcatggata
      781 atcctatggc ggaactgatt tgcatcaatt gcggacaatg tattaaccgt tgtccgaccg
      841 gcgctttgca tgccaacgac ccaagtgatg cgatctgggc tgctattgat gatccgacta
      901 aacatgtcgt gattcagacc gctccggcgc cgcgttccgc cattggcgag tgtttcggtt
      961 tacctgctgg cacccccctg acttttgaaa tgaataccgc tatgcgttac tgcggtttcg
     1021 ataaagtttt cgatactaac ttttccgccg atttaaccat tattgaagag ggtaccgaac
     1081 taattttgcg gctttataag gctttagtaa agaaagaacc cgtgtctctg ccgctgttta
     1141 cttcctgttc gcctggatgg gtaaaatata tcgagcattt ttacccggaa atgctatcgc
     1201 acctgtcatc ggccaagagt ccccaacaga tgttcggcgc cgtcatcaaa acctattatg
     1261 ctgagcttaa taacatcgat cctgccgata ttgttaccgt tgcgctgatg ccctgtacgg
     1321 ccaagaaatt cgaatgcaat cgtcctgaga tgaattcatc cggttttaaa gatattgatt
     1381 acggcttgac gacgcgcgaa ttggccggga tgttccagga agccggaatt gatttaccta
     1441 atatgcctaa atcagatttc gacgaaccgt tcggaactcg caccggctcg ggcgtcattt
     1501 tcggtgcaac cggtggcgtt atggaatcag ctattcggac tgtctatgaa ttaatcaccg
     1561 gtgatcgtat tgataaccta ttcgaacggg ctcgcataat gcccgtgcgc ggtttcgaag
     1621 gcatccgtta cgctgagctt cccattgcta aagtcggtcc ggtgccggac ttactaaagc
     1681 atttgattcc taattgggat tggctgagag gtgcaactct gaaagtagcg gtagcacatg
     1741 gaactgctaa cgccaagaag gtgcttgata atattaaatc cggcggcaaa ttaagtgaat
     1801 gccacttcat cgaatttatg gcttgcccgg gtggttgtct gggtggtggt ggaatgccga
     1861 ttccgaccaa tgaagaaatt cgagccgctc gagcgcaggc tatctatgct gaggatgaag
     1921 ccagcgaggt tcgcaaatcc tatgaaaatc cagccgtggt caggttatac aatgaatttc
     1981 tcgttgatgg accctgtggc cataaaagtc ataaactctt gcatacttac tatacgccac
     2041 gtggtaaaaa attagtgtaa caatcatcct cctctttatc tctaagaagg gcaatctttt
     2101 agtagtttgc ccttttttaa taaatgaggt cgtagtaatt ttgcaattga gtaattgaaa
     2161 aaaagaattc atttcataga ggataaaatg ccgaataggt ttatttattt agatcacaat
     2221 gctaccactc cattacaccc ggaagttaaa aatactatta ccgatatgtt cgatgtattt
     2281 ggcaacccct ccagtctcca caccgtcggt cggatcgctc gccaaaaaat cgaacaagcc
     2341 cgtgaaattg tcgcaaattt catcggcgcc gcgcccgatg aaattatctt cgtcggaagc
     2401 ggtagtgaag ccaacaattc tgtccttaat agtctctcct gcccttcgca ggtttgtcgc
     2461 ctcgaacgca acctgcgcga tgaaatcatc acaacctcta tcgaacaccc ttgcgttctg
     2521 gaaacttcca aatgtttacg agaacgcggt acgactgttc attatttgcc agtggatatt
     2581 accggaaaag ttaaactcga tgtacttaag gaattactgt cagaaaaaac cgcattggtt
     2641 tcggttatgt acgccaataa tgaaatcggt actattcaag atataaaagc tattgcggaa
     2701 atagcccatg ctcaaggcgc ttttttccat accgatgccg ttcaagccgt cggtaaaatt
     2761 cccgttaacg ttcaagacct cgacgttgat ttcttagcat tttctgccca caaaatctat
     2821 ggcccgaaag gggtcggggc actttatgtc aaacgtggga ctcccttctg tcccttaatt
     2881 cgcggcggtc atcaagaacg cggccgccgt gctggaactg aaaacacgat cggcattatg
     2941 gcttttgcca aagccattga aatgcgtaaa caagaaatgt ctgatgaagc cgtacgtctg
     3001 cgccgtttta aaaaaatgtt aattgacggg attaagtata aaattccaga tgtgcaattt
     3061 aatggtcatc ccaccgatac tttgcccgga acactgaata tctcctttgc cggggcggaa
     3121 ggcgaatcaa ttttactcta cttagacctc gaaggaattg gggtttcaac cggctctgcc
     3181 tgtgcctccg gttcgcttga gccttcacac gtaatcctcg ccacaggcgt tggcgcggaa
     3241 ctggcccacg gctcgatccg cattagtatg gggcgtgaga atactactga cgacatttat
     3301 tataccgttt cggctctgga gcgcgtggta aaaaaggtgc gtgatatgtc aacaacttat
     3361 cagagtaaat tggtatgagc aatcaatctg gctggctata ctccgaaata gttaaagacc
     3421 attttatgaa tcctcgtaat attctaaggg atgacgagaa ctttgtcgcc gatggggtcg
     3481 gtattaccgg caatatcaaa tgcggcgacg agatgcagtt tctcatccag gttgacaagc
     3541 aaaaaggtgt tataacagat tgccgctggc gaacttacgg ttgtgccagt gccatcgcca
     3601 gcacttccgt tctctcggaa atggtcaaag gtatgaaact tgaagatgct tatcgtataa
     3661 ctcccaaaga tgttgtgaaa aatctcggca gtttacccga acacaaaatc cactgctcgg
     3721 tgctaggtga caaagctctg cgcgccgcta ttgacgatta ttatcggcgt aacggtatga
     3781 acgaaaaaat caagcacgaa gaagcccgcg ttatctgtca atgtatgaac gttaccgatc
     3841 atgaaatcga agaggcggtt ctggagggtg cccgcaccta tcaagaatta caggaacgca
     3901 ctaaactcgg cacggtttgc ggtcaatgtc aggaagaagc caccgaacta cttaaaaaat
     3961 atatcgcaaa acacttcggc gataaccgga aataatcctg aatatcttat tttaagtact
     4021 tttaagtgct ttaaagttct ataagaaaat ttctgccata aggttgtttt taagcattaa
     4081 cgcatgacag actgattata tggatgttta gaattacatt ttgtcgcaaa gataatttaa
     4141 tttattataa gcacttaagc gcgcttaaat gaacttaatt gcttagcgca ctctgcggtt
     4201 ggctattttc ttaagattta cgttgattta tagattgctg agttatttga gtttgtaact
     4261 tcttaaaccc cagcttactt ctcgcattgt aactttaata attccccaaa acggaaccgc
     4321 gaacagcatc ccgaacatac ctgcaatatt accaccaatt atcaccgcga tcacgacagt
     4381 tatcggatgc atattgacgc tcttagagac taccatcggt gagaccagat tattatcaat
     4441 gagttgcacg agaacaaata ccacgataat ccaaaaaatg atatatccga cgttattcgg
     4501 attattcaga atagtaagta caatagcggg aacagcaccg acatatggcc cgagataagg
     4561 aataagatta gcgacgccag cgatcattcc gataacaaaa aaatacggca ccggattatc
     4621 aaataccaaa ttgataatga ataagccgat taccgacaat aagccgacga tcaaagcgtc
     4681 tatcgcttgc ccctgaatat atttcgtaac ctgagaatca actttgtgca agacatttaa
     4741 aaataattcg aaataacgat taggcacctg actgattagt gctctcttaa ataatcgtcg
     4801 atccttgatc aaaaaaaagg ttataattgg tacaattcca acgaatgcta cataagtgcc
     4861 ggcatttttt aaaaaacccg cgactttcac cagtagcgtg cctccgattt gttctgcctt
     4921 atttatcaga tcgcgcgcca gatcgggatt gttgaatttt tcctgaaaaa attgctcaag
     4981 ttgaatggtc aagttactaa ttaaatcact ttttaaagta gcggagatgg attcaaccgc
     5041 ttttattacg gtcggaataa tgccgataat taaagcaata atgccaccaa gaataacagc
     5101 cataatggtt agaattgcta aggctcggtt aaatccaata ctttcaagat aatctactaa
     5161 tggattgaga atcaccgaaa taagccaggc aattaacaac attaataaca cagttttaac
     5221 ataggagaag atcgaaaata taaatgccac tcccataata attaaaaata tacgccagat
     5281 ggttttactt aactggcttg ttggagtagc cgatgtatta ttcatggcgt ttcgccttca
     5341 tttcacgttg tagattagtc aattgttcat ctgtttttcg taaacgctca ccaactactc
     5401 gggataaagc ccagaggatt ttgattccca tacgaggttt gcgatacaat aaatccacta
     5461 aatccggtcg gaaaaaacca attatgtcag aatcaaccat cgcgattgcc gaggctgaac
     5521 gtggtttctc atcgagtaga gccatatccc caaaaaaagc gcctttttca agttccgcta
     5581 atttcgtccg ggttccattt tctggttctt cgacaatttc caccgctcca ttaacgataa
     5641 tatacattcc aagccccggg tcacccattc ggaatatcgg ctcgcctttt ttatatttac
     5701 gatggtaaat tattttttcc acctctttta actccttaga atccagatca gagaagatag
     5761 gcactttagc cagaatttgc attatatcgc tcatttccga tttatttttg gctgatattg
     5821 ggtcccataa tggatgattc attttatcta ttccttttac ttcattttcc aataatatcg
     5881 aggtaatata caaacaattt atttatacaa acaattccaa taagtcaaga cttctccaat
     5941 atttcctatc ctttgccccc taattattga tgttcaaaga taataaagca aaaatacaat
     6001 ttttctccat cacaatttat tcactaaaaa atattaactc gaagaggtta aaccttctaa
     6061 aaattgagga atgtcctgta acaagtgact tgctattcaa ctcgacatta cttattttaa
     6121 gccgtttatt atgagtatga tgttattaat tagaaacttt ctgataatca tagttttact
     6181 gttccttagc ccggttcagg ttaactcaca ggaaaaagac ctggatcaaa cgattgagta
     6241 tcaatcggag caactggaga aaatccgtaa agaaatccgc aattataaaa gtgatcttaa
     6301 aaaagccgag tctaaagaga aatcaatttt gactcaaatt gatgaaaccg aacgcagtat
     6361 cagcctgacc gagaaactca ttgcccagct taatcgcgaa cagattcaaa aagagcgcga
     6421 aatcgagcaa atagagcgtt caattaagca attggaatcc gatttaaata ctcttaaaaa
     6481 cgattttgcc aagcgccttg tttatcttta taaaaatggc aaatacaacg acctggaatt
     6541 gatcttaacc gctaaatcca tcaatcaggc tttttatcgt tataaatatc tgcgcattct
     6601 ggctgatatt gaccgccgta cttgtactct tattcggaaa gatatcaata ctataaatca
     6661 aaaaaaacaa caactaaatc aagagctaag aaataaagaa aaaatcatca atgaaaaaaa
     6721 agaccttatg gctaatcttc aacagcaaaa gcgtcgccat aatgtccagc tcgcaaaagc
     6781 ccgtcagaat aaatcaaata tcgccgccca aattaaagaa aaagaaaaaa acgccgccga
     6841 aatttccaaa ttgattgcta atctggacaa ggagcgcgaa aaacgtaaca aagagctcga
     6901 acgtcagcgc gctctatccg gagttgcgga aagcaatcca tttcttgcaa gccagggcga
     6961 attaatctgg ccagccaaag gtgaaatcgt ctcgcgcttt ggtattcaaa agcatcctac
     7021 gctaaaaaca atcaccgata atcccggtat tgacataaaa gtaaaactcg gaacgccggt
     7081 ggtttccgtc ctggacggag ttattacaac tattacttat attcgcggct tcgggaatac
     7141 aatcatcatt gatcacggct ccggttttta ttcggtttat tcccatgtcg aaaatattcg
     7201 cgtcgctgaa aatgattatg ttacagtcaa ttcagtcatc gccgaggttg gtcagtccgg
     7261 ctcactgagc ggcccaatgt tgcattttga aatctggcgt aacaaaacaa aactcaatcc
     7321 cgaagaatgg ctgaataaaa aatgacacta ttccccctta ttaatcagac cgaaaaccga
     7381 aatctattac gcaacatcgt cgccactggc agagtagcaa acacctacct attctatggc
     7441 ccggaaggta gcggaacaga aggcttcgcc cttgaatttg cggcaatgct gaactgctct
     7501 tcaaaaagtg aacgaccatg tggtatctgt cagtcttgta aacagatgaa aaccctcgaa
     7561 catcctaacc taaccctggt tttcccgatt ccgggttcac cagactctaa accaggcgat
     7621 tcgcctttca aaaagttcaa agaaggggaa atggacgaaa tccggcaaat tattcagaat
     7681 aaaagtcgta atccctacga taaaattata gtcaaaaagg cgctcgtcat tcccatcaat
     7741 tttatccgtg aaattaaacg ccatatctat ctcgctcctc agaatggcgg tcagaaagtc
     7801 gtagtcattt ttgatgcgca tttaatgaac gatgaagccg ctaacgcttt cctgaaaatc
     7861 ctcgaagaac cacccgacaa ttctactata atcctctcca ccgcttatcc cgatcagctc
     7921 ctgccgaccg ttcaatcacg ctgtcagtgg ctatacttcc cgcctttgcc agaagctgaa
     7981 ctgaaagaat ttctcataaa aaaagggtat ccacttgaaa atatccggtt ggctattcgt
     8041 ctctcaggcg gcaatgtcag ccagcttact caactaattg aacacgattt atcagatatt
     8101 aaagaattga ccttggcaat tttcctggat atcgccgcct ggaaagtttc caatttatat
     8161 aatcatattc aaaaaatgac gactatctat cgccaagacc ctgaaaaatt cgcacaactg
     8221 atgcaatccc tgattttctg gattcgcgat gccgaattga ttcgccaggg acagccaatt
     8281 gatgatatta tccatctcga ttatgccgaa agactaacta attttgttaa aaaatttccg
     8341 gatttcgacg cccaaaatat gaaggtttcc gtcgagaatt gtgttgattt cattaataag
     8401 aatgtctata ttaacctcgc cttaattgag atgttcttta aactgaaaaa gcaacttaat
     8461 aattctaaat aacaatgaat accagcgaca aactcatcaa aataaaattc aaaggcggta
     8521 atcgtcagtt atgtcagaac ccgctcgact ttccagttca gccgaatgat aaaatggtcg
     8581 tatccgccga tcgtggcgaa gaactcggtt gtgccctatc atcgggctca cctaaaaatt
     8641 taccggaaga attgggtgaa attccctctc tcttgcgtaa agccaccccg caagacctat
     8701 cgcagagcaa cgaaaatcgt ttacaagaaa aaatggctct ggaattctgc gtcgctaaaa
     8761 ttagcgaatt aaatctgcca atgaaattaa ttgacgttga tgtgcgtctt gaccggcgta
     8821 aaattgtatt taatttcacg gcagatgaac gaattgattt ccgtgaatta gtaaaactct
     8881 tagcggctga atttaaaact cgcatcgaaa tgcgccagat tagcagtcgc gaagaaatga
     8941 aacgtactga cggagtcggg ctctgcggct tgagactatg ctgtgccagt ttcattgatg
     9001 atttcgatcc aatcagtact caattcgcta aggaacaaaa tttgccaatg aatcccgcca
     9061 aaatttctgg ctgttgcggc aaattgaagt gctgttaccg ctacgaacat gacttttacg
     9121 aagaagtatt aaaaaattat cctgcttatg gcaccaaagt caaagtcgcc gataaaaccg
     9181 gcattctcga aaaaattgat attttcgctc aaactctcac tattcgctac gacgacaata
     9241 ctgccgaaga ctttccatta accgatttca atcaaaaaat aactacagtc caataatttt
     9301 caccaaacta tgaataatca agaacatttc tttgttacga ctcccatcta ttacgtcaac
     9361 gacaagccgc atattggtca cgcttatacg accattctcg ctgatgtgtt ggcgcgtttt
     9421 cagcggagta acggtgtcga aactttcttc ttaaccggtg tggatgaaca cggccagaaa
     9481 gttcaacaag ccgccgcctc tgccggtatt tctcctcagg aatattgtga ccgaatggtt
     9541 gtctatttcc gcgaagcctg gcaaaaatta ggaatcaaat acgacttttt tatccgcacg
     9601 accgaccagt ttcatgtccg tgctgttcag caagttctac aagatttgtt caataagggt
     9661 gaaatctacc tgcacgaata tggcggctat tactgcgtcg gctgtgaacg attttatact
     9721 ccgaaagaac tggttaatgg ccaatgtccc cagcacttga aagagccgga ttttatcaaa
     9781 gaaaagaatt atttcttccg gatgagtaaa taccaggact ggctaatcgg ttacattcaa
     9841 gaccatccga attttattcg accggagagc cgcaaaaacg aagtactggg ctttttgaaa
     9901 aatccattgg gcgatctgtg tatttcccgc cccaaatccc gcctaagctg gggcatcgaa
     9961 ttgccgttcg attcgggtta tgtcacctat gtctggttcg atgctttgct gaattatatt
    10021 acggcgattg gctatccgaa cgaccggcaa cgtttcgcaa aatggtggcc tgctgatttt
    10081 catctgattg gcaaagacat cgttacaact cattgcgttt actggccaac tattttgaaa
    10141 gcaatggatt taccgatgcc gaaaaccatc ttcgctcacg gctggtggat gattgacgaa
    10201 accaaaatgt caaaatcact caaaaatatt gtcgagccgc tcgatctggt tgacgcttat
    10261 ggcgttgacc cggtgcgcta tttcctgatg cgcgatatgg tgctcggaca ggacgccaat
    10321 ttcagcgaag gaatcttcat caagcgctac aattccgaac tggctaatga cttcggcaat
    10381 ctcgttagtc gcgtgactac gttaataaat aaaaattttt cgcaaattcc agcgcccgag
    10441 caattaagta atgccgaaaa cgaaatccaa caaaccgctg agcaattgcc taacattact
    10501 cataaactct tcagcgaatt gaaaataacc gaagccttag atgaaatcat taattacata
    10561 cgatctataa acaaatatct agaagtgcgt cagccctggc acctcattaa aagcgataaa
    10621 accgccgccg gaaccgtgct ttttaccgcg ctggaaggct tgcgtctcgc cgcgacacta
    10681 ctcgaaccga ttatgccgca aaaaattgcg gaattgttga acgctctgcg ggttgacgaa
    10741 aataaaccgc ctttcgaatg gggacgaatc aagagcggtg tgactctcgg cgagcttcca
    10801 attctcttcc ctcgcattga taaaattgcc aaaccgatgg tcgaaccgcc ttccacccca
    10861 ccccaaccgg ctgaaccatt aatcacaatt gatgattttg ccaaaattaa actcaccacc
    10921 gctaaggttc tgaccgccga aaaagttccg gatgccacta aattactaaa attgacaatt
    10981 gacatcggca cggaaaaacg tcaaatcatc gctggaatcg ccgagtatta ttcaccggag
    11041 caaattatcg ggaaaacgat tgtcgtcgtc actaatctcc aaccggcaac gattcgcggc
    11101 ataaaaagcg aaggtatgtt attagccgcc aaaaaagacg gcaaactgac tttactcagc
    11161 agtctcgagc cgatcgaacc gggcgcaaaa atcagctgat ttaatgttcg ttgatactca
    11221 cgcgcatctc acctaccctg atctgattgt taaactcgat gaaattatta agcgcgctcg
    11281 cgatgccgga gtcactcgca ttgtcaacat tgctaccgac
//
LOCUS       DAOOMU010000075        11301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_6446_length_11301_cov_16.336564, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000075 DAOOMU010000000
VERSION     DAOOMU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6446_length_11301_cov_16.336564"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..879
                     /locus_tag="PLI55_07670"
     CDS             91..879
                     /locus_tag="PLI55_07670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPB00752.1"
                     /translation="MDDLQLQQKLSATALNAALEAGKILKQKVNTALKIDYKGRVNLI
                     TEADRMSENSIIESIRRQFPEHQILAEESPLIETSSDYKWIIDPLDGTTNFVHGFAFF
                     SVSIGLEYQKELLLGVVHAPLLGETFTAMKNQGAFLNGQPIRVSQVRKLADSLLATGF
                     PYENNATFRRNFEHFQRIYPLTQGVRRVGSAAIDLCYTACGRFDGFWELDLNAWDVAA
                     GALILQEAGGKTVNMDGSAFSIYARQILATNGLIEAELLPIFKE"
     gene            complement(883..2136)
                     /locus_tag="PLI55_07675"
     CDS             complement(883..2136)
                     /locus_tag="PLI55_07675"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPB00753.1"
                     /translation="MIPTGLAILIIAICVLGEGFFSGSEIALVSVDRIRLRHDAKMGQ
                     KDAKLVINLLKRPEWVLGTTLIGTNICTVTSTMLATAIAYRYFQGAGVFVAIFCMSLI
                     NWIFAEIVPKSVFQQYADVIVPKIARPLRIMSLVLAPLVRVFSGIASYITTALGGKLS
                     HKLPFISKEELKILMKISDKSDVNPNEREMIGRLLSFKEKKAEDVMVPLIQVTALPED
                     ASVPEAARIISESKHRRLPVYRERVDNIIGILNSFDVIGENNNTPIRRFIRPAFYAPP
                     NMSINVLFDQMKKSGNNMAIIVDEYGGAEGIVTIEDILEEVVGKLEDEYDQVRPRYKI
                     KKDGTIVVSGKMEIQELNDKFGLNLPEGDYDTIGGLIIAHLQKIPRHGERLILPSVAL
                     TVTRASQRVIHEVAIRKNGSTEVTN"
     gene            complement(2133..3392)
                     /locus_tag="PLI55_07680"
     CDS             complement(2133..3392)
                     /locus_tag="PLI55_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPB00754.1"
                     /translation="MVLVTIEIIILVILLGLSGFFSSSETALFSLSKIQREKIQESDP
                     AQGKIINDLLNTPRSLIITILLGNEFVNICFSSLFTLIVIQIAGTVLPWANYLIALPL
                     VLLIGEITPKTLAIKNNERLSLKFARPLSLVMVIFTPLRWFIRNISDRIVNLIVSKRD
                     QRENILTEDVIRSILEDSEKEGVIDSDESEMISNVFDFGDLIAADVMTPRSLIFSLPV
                     DMALPEVITAIKNRRISKVPIYEGNPDNIIGVLFATDLIGISLNTSNDETKPINLRNL
                     LRPPFFVPKTKRVDELFHIFQRKRISMAIVRDEDGIVQGLITIEDLLEEIFGEISDEF
                     EIKDEQFEKINDRLYRVRGTLPIVSFNELLNARIPVDEFETIGGFVSSLFDELPTVNA
                     QKIYDGWLFTVEKIENNRIEILRVKRL"
     gene            complement(3458..4951)
                     /locus_tag="PLI55_07685"
     CDS             complement(3458..4951)
                     /locus_tag="PLI55_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPB00755.1"
                     /translation="MFNITDLIVVVTSVFCLLATAESQEYSGEKYKLDARKDTIWTKG
                     IPVEYSDFPEAEKIAIAVMELDANGVSSAEAKALTDRLRIELFNAGIFQVMERDKMNK
                     ILDEMQFQMSGCTSDECVVEVGKIVGVKKIIAGSVSKVGEIYSVSIRLIDVATSRIEA
                     TAIEDIEGALGAVLTEAIPSVARQISGLEHQTKELKGKKTAFKISTEPYGASVFIDDV
                     YYGIAPIEVEVVPSVTHRLTIVNEGYEKWEHYYDIKKDQVLEINVALSKKLQPVTKNV
                     AEKPKRQFKTGFKIRYSDFHIVDKINNQVQIMNAKMAEGNELFHRRQPGAYVFDEINN
                     FSGIELSNLRQSDEVVGFDFGLGIYRSSFTQWIKNFDATYSEPTLIFWMPSVNLNLRL
                     APIRFPLFYPFCNVGFGYDVLIIDAVEDNKSMGSPIFHSWGLMYGVGAELRLFKFIGL
                     ACDWNHYNMKYKLMNIENKATQRFKDASISQMDLSGNTVGLSLNLYY"
     gene            complement(5174..6460)
                     /locus_tag="PLI55_07690"
     CDS             complement(5174..6460)
                     /locus_tag="PLI55_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB00756.1"
                     /translation="MKKLLLILMLTIYFVFFTQRIEAQNELWDRIFVFPENYRIYTLS
                     FSDSLNGWAGGETIGPVGGVILNTKDGGQTWSEQFVYPEYSWFNNIDFVDSLNGWEAG
                     LHCLFKTSNGGKEWIRVADSLFDFDIYMGLSDLQSVSFADTAFGIITGTESTIAITLD
                     GGNTWNCATINPENVFIDTVYYGVVLSAGKACVTGRGGVAVSADSGKTWHITHNEDRS
                     YIKCSFIENGYGWVLSRDGQIQKTRDYGESWIFCKQIFEDGVDYAVDISFLDSLTGWV
                     IANNGEIWETNDGGYNWLSSTISQDTALAVITSATNNATFVLNKANSLYKYKRFSAIT
                     EFFKKLPDCYKLCHNYPNPFNSSTNIKYSIPSKSPVKIQVIDVNGNLLETLVDRSHEP
                     GNYQITWDASGFSSGIYFYRIQAGDFIEARKCLLIK"
     assembly_gap    6757..6856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6857..7056)
                     /locus_tag="PLI55_07695"
     CDS             complement(<6857..7056)
                     /locus_tag="PLI55_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPB00757.1"
                     /translation="MHLTILGSGSFLPNEKRNCSGYLLDIGDETLLLDGGSGTLRQIV
                     KAERSVLEVGRVFYSHFHLDHIA"
     gene            7193..>8139
                     /gene="add"
                     /locus_tag="PLI55_07700"
     CDS             7193..>8139
                     /gene="add"
                     /locus_tag="PLI55_07700"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HPB00758.1"
                     /translation="MNKNNTGEIIQAMPKIELHLHLEGAFPLESLLRLIQKYGSNEVH
                     NIEELRQKFVFTDFAHFIETWYWKSDFYREPSDIEDMAYSTIASLSLQNIIYAEVYFS
                     PFDFIKPQMPFPVVTEAVISGVRRAESEYPIRIGLIADIVRNHGHETALARLDKITQY
                     QNEVLGIGLGGSEKEFPAKLFAEVYTEAQKRGFRLVAHAGEADGPNSIWDALRLLHAE
                     RIGHGVRAVEDPDLMEYLRLNQIPLEVCVTSNLKTKIFNSAAEHPVRRLFDYGLLITI
                     NSDDPTMFGSNLTDEFNFIANEYNFTLPEIKKLTQNAIQA"
     assembly_gap    8140..8239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8240..10096
                     /locus_tag="PLI55_07705"
     CDS             <8240..10096
                     /locus_tag="PLI55_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB00759.1"
                     /translation="DSLLFTRIGYGEQEIVLGNLYEPVKIILPFTPVNLDSVTVAGQI
                     FPRQLSGINFSRVERTEDLGTVEHRRLWTTIPGLYLKSYGGPTGISTLSLDGSPSTHT
                     RVVIAGFDLTSAQNGQMDISQLPPTFISSINYSPEQAGNTSAENSEGLLNIEPNMSPT
                     GFTCSAGSYGKLNLSANLDIRKTVWNSNILLGHNYYRGDYEVTWRDQNFKRSNNSLQQ
                     NYFSWQSGYVFRSKAFARLIAFYSEQERGVAGQVWNPSPDAFRQDALGFIGLKIGWTV
                     KAGHSYIQTLHRHSDERYVNPTIAINSRHVVNTDQIIYHANRSILNFNLDFTSDLKSD
                     RLQSKQAGHHSRITLTNILALPWNFHPNYELRPWVKFEHSPDLYQKATWGGRIAYRGS
                     GILNTISGSYGQYYAYPTFNDLYWQPGGNPNLKPDETTKYEIDLILQILPELKLVLNS
                     YYKKDKNLIQWTPVQSYWQPSNISQAERKGIKVILNWSLPNLPVDGFLQGILTHSCNL
                     TKGNNYQKPLRYTPKESYAAGLNFNFKHIAFRSTIEYVGARIAMYNWPEDIILAEYLV
                     LNANLAYKIDTTIGRFTIVAGCDNITDESFESMQGYPEPGRAFTGTIEYNPK"
     gene            10115..>11301
                     /locus_tag="PLI55_07710"
     CDS             10115..>11301
                     /locus_tag="PLI55_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00760.1"
                     /translation="MKQKLTLLAIITIFQISLAQNFTYILCEGNFNTTNASLWQLTGT
                     SELIGPVHWNFTDNPLGDVGQNLKVYQEKLFIVMNNSNSLEVMSLSNGATYERTIDLP
                     FAGPRDIAFVNNHAYITCWYLPGILSLDLNTWQIDDTIFINGLPDNIIADDGKLYTSI
                     AMNTDWSPADKVLALESVSGEFTPADTFTVITGPNQLLLYENKLFVLSTYYDDTWNTY
                     TGTSCINLETNQVTKREQGLSFNFCDDLALINDKIYRSYNGGVAPLADSLTIDTNKII
                     GNYAGIYSMAFDGKNIFIGLSDYSAPDQVVILDTLGNEINTLNVGVCPGSFAFYQQPN
                     AEIFTDEKNGILSDFLLMTNYPNPFNSNTIIPFYLKNTNLVQLTIYNLKGELIAKLTD
                     DYFT"
BASE COUNT         3364 a         2470 c         2030 g         3237 t
ORIGIN      
        1 atttgcgacc tttaataatt aaaacaaact acacttagtc taaaaattgg attaattaac
       61 tataacatca atgaaattaa ggaaataatg atggacgatt tacaattgca acagaaatta
      121 tcagcgacgg ctttaaacgc cgcgctcgag gcgggaaaaa ttctcaaaca aaaagtcaat
      181 accgccttga aaattgacta taaaggacgt gtcaatctaa ttaccgaagc tgatcgtatg
      241 tcggaaaatt ctattatcga gtcaatccgc cgtcaatttc ccgagcatca gattctggcg
      301 gaagaaagtc cgctgataga aacttcttct gattacaagt ggatcataga tccgctggat
      361 ggtacaacca attttgtgca cggttttgca ttcttttctg tttcaattgg actggaatat
      421 caaaaagaat tgttattagg cgttgttcac gctcctttac tgggcgaaac ttttaccgcc
      481 atgaaaaatc agggtgcttt tcttaacgga caacctattc gagtttcgca ggtaagaaaa
      541 cttgctgata gtcttcttgc taccggtttc ccttacgaaa ataacgccac tttccgacgt
      601 aatttcgagc atttccagcg catttacccg cttactcagg gtgttcggcg tgtcggttcg
      661 gcggcaattg atttgtgtta tactgcctgt ggccgctttg atggattctg ggagttggat
      721 ttaaacgcct gggacgtagc cgctggagcg ctaattctcc aagaagccgg tggcaaaacg
      781 gtaaatatgg acggttcggc attttcaatt tatgcccgtc agattttagc gacgaatggt
      841 ttaatcgaag ccgaattatt gccgattttc aaagaataaa ttctaattag ttacttcagt
      901 cgaaccattt ttccgaatgg cgacctcatg aatcacgcgt tgagaagcgc gtgttaccgt
      961 taatgcaaca gatggcagaa ttaatctttc tccgtgacgc ggaatttttt gcaaatgagc
     1021 aataatgagc ccgccaatag tatcgtaatc accttctggc aaattcagtc caaatttgtc
     1081 attgagttcc tgaatttcca ttttgccaga gacaacaatc gtcccatcct ttttaatttt
     1141 atagcgagga cggacctgat catattcatc ttctaatttg ccgacaacct cttctagaat
     1201 gtcctcaata gtcactatgc cctccgcgcc gccatattcg tcaacaatta ttgccatatt
     1261 gttgccgctt tttttcattt gatcgaacaa aacgtttatg ctcatattgg gtggtgcata
     1321 aaaagccgga cgtataaatc tcctgattgg agtattgtta ttttcaccga tgacatcgaa
     1381 actattcaat atgccaatga tattgtcaac ccgttcacga tacaccggca gacgacgatg
     1441 cttactttcg gaaatgatgc gcgccgcttc cggaacgctg gcgtcctcag gcagagcagt
     1501 aacttgaatg agcggtacca ttacatcttc agcctttttt tctttaaatg ataaaagtcg
     1561 gccgatcatt tcacgttcat taggattaac gtcactttta tcgcttattt tcatcaggat
     1621 tttcagctct tctttactga taaatggcaa tttgtgactg agctttccgc caagcgcggt
     1681 agtgatgtag gaggcaattc ctgaaaaaac acgaaccaac ggcgccagta ctaaagacat
     1741 tatccttaat ggacgagcta ttttcggaac aattacatcg gcatattgct ggaaaacgct
     1801 tttaggaacg atttcagcaa aaatccaatt gatcaaactc atacaaaaaa ttgcgacgaa
     1861 tactccggcg ccctgaaaat atctataggc tatagccgtt gccagcatcg tgctggtcac
     1921 cgtgcaaata ttcgtgccga tcaaggtcgt ccccaaaacc cattccggtc gttttaaaag
     1981 atttatgact aacttagcat ctttttggcc cattttggca tcgtggcgga gacggattcg
     2041 atccacggat accagtgcaa tttccgaacc ggagaaaaac ccttccccga gcacgcaaat
     2101 agctataatt agaattgcca aaccggtcgg tatcacaatc gcttcacccg caagatttcg
     2161 atacggttat tttctatttt ctcaacggtg aatagccagc cgtcgtaaat cttttgagca
     2221 ttaacagtag gtaattcatc aaataaactg gatacaaatc caccgatagt ctcaaattca
     2281 tctaccggaa ttctggcgtt caataattca ttaaaactaa ctatgggcag agtaccacgc
     2341 actcgataga gccgatcatt aattttctca aattgctcat cttttatttc gaattcatcc
     2401 gaaatttcac cgaaaatttc ttccagtaaa tcctcaatgg taatcaagcc ttgcactatg
     2461 ccgtcttcat cccgaacaat agccattgaa attcgctttc tttgaaaaat atggaacagt
     2521 tcatccactc gtttggtttt gggtacaaaa aacggtgggc gcagaagatt ccgaagattt
     2581 atcggcttag tttcatcgtt actggtattc agcgaaatgc ctattaaatc agtagcaaat
     2641 aatactccga taatattgtc aggattgcct tcataaatcg ggactttaga tatacgccta
     2701 ttcttgatgg cggtgataac ttccggtagc gccatatcaa ccggcagact aaaaattaaa
     2761 ctgcggggtg tcataacatc ggcggcaatc aaatcgccaa aatcgaatac attagaaatc
     2821 atttcactct catcggaatc aataacgcct tctttttcac tgtcttccaa tattgagcga
     2881 ataacatctt cagtcaaaat attttcgcgc tgatctcttt tcgatacgat cagattaacg
     2941 attcggtcgg aaatattcct gataaaccaa cgtaatggcg taaaaattac cataaccagc
     3001 gaaagcggtc gggcaaattt tagggacaaa cgttcgttat tcttgatagc caaggtctta
     3061 ggcgttattt cacctatcag cagaactagc ggcaaagcaa tcaaataatt tgcccacggc
     3121 aaaaccgttc cggcaatctg gataacaatc agagtaaaca aagatgaaaa acaaatatta
     3181 acaaattcat tacccagaag aatcgtaata atcagcgagc ggggtgtatt aaggagatcg
     3241 ttgattattt taccctgggc gggatcgctc tcttgaattt tctcacgttg aattttcgac
     3301 aaggaaaaaa gagccgtctc cgagctggag aagaatccgg acaatcccag caaaatgacc
     3361 agtataatta tttcaatcgt aaccaaaacc acaaattaat ataataaata gcggtgatat
     3421 aaaataccaa ccagaaaatt tttacttcaa tttacttcta ataatataga ttgagagata
     3481 gaccgacagt atttccggat aaatccattt gagaaatgct ggcatcttta aagcgttgcg
     3541 tggctttgtt ttctatattc attaatttgt atttcatatt ataatgattc cagtcgcatg
     3601 ctaatccaat aaatttgaac aggcggagtt cggcgccaac tccgtacatt aacccccagc
     3661 tgtgaaaaat cggactaccc attgatttat tatcttcaac tgcatctatg attagcacat
     3721 cataaccgaa cccgacattg caaaacggat aaaataaagg aaaacgaata ggcgccaaac
     3781 gcaaattcag attcaccgac ggcatccaga atattaaagt cggctctgaa taggtagcat
     3841 caaaattctt aatccattgg gtaaaactac tgcgatagat acccagaccg aaatcaaaac
     3901 caactacttc atccgattgc cttaaattgg ataattcgat gcctgagaaa ttgttaatct
     3961 catcaaaaac gtaagctcct ggttgtctgc gatgaaaaag ttcgttaccc tccgccattt
     4021 tggcattcat tatctgtact tgattattga ttttatcaac aatatgaaaa tcagaataac
     4081 gaattttaaa acctgtttta aattgacgtt tcggcttttc ggctacattc tttgtaaccg
     4141 gttggagttt tttagaaaga gcaacgttaa tttccagtac ttgatccttt tttatatcgt
     4201 aataatgttc ccatttctcg tagccttcgt taacaatggt caatctatga gtaacgctgg
     4261 gaacaacttc aacttcgatg ggagcaattc catagtaaac atcatctata aatactgaag
     4321 cgccgtaagg ttcagtgctt attttgaagg ctgttttttt accttttaat tctttagtct
     4381 gatgctcgag gccagaaatt tgacgagcta cagatggaat tgcttcggtc aaaactgctc
     4441 caagtgctcc ctcaatgtct tcaatagccg tggcttcgat ccggctggtt gcgacatcaa
     4501 ttaatcggat gctcaccgaa taaatttcac cgactttgct gacggaacca gcgataattt
     4561 tcttgacgcc tacgattttt ccgacttcga ccacacattc atcggaagta caaccactca
     4621 tttgaaattg catttcatcg agaattttat tcatcttatc gcgttccata acctggaaaa
     4681 taccggcgtt gaacagctcg attcgtaagc gatcagtcag cgcttttgcc tccgccgaag
     4741 aaacgccatt agcatctaat tccataactg caatggcgat tttctccgcc tctggaaagt
     4801 cagaatattc aactggaatt cctttagtcc aaatagtgtc tttacgagca tctaatttat
     4861 acttttctcc actatattct tgagattcag ccgttgccaa tagacaaaaa accgatgtta
     4921 ccactacaat taagtcggtt atattgaaca tttcctcatc ataaataaat cgtatatcct
     4981 caaaattttc gggtaatata accgcaatcg gtatttgtag atatgatatt tctcacactt
     5041 tttagtgcta tgattcatcg aatctcgagt ttactcaatt ggatttaatc tttcccagcc
     5101 cttaattcta aaaatccaag gttagacatt tataacctac cgcctcttat attctgaata
     5161 ttattggcaa tatttacttg attaacaaac actttcgagc ttcgataaaa tcaccggctt
     5221 gaatgcgata gaaataaatg ccagatgaaa atcccgatgc gtcccaggtt atttggtaat
     5281 tgccaggctc atgtgacctg tcaaccaacg tctcaagcaa attaccattt acgtcaatca
     5341 cctgaatttt aaccggtgat ttgctcggta ttgaatattt aatattagtg gatgaattga
     5401 aaggattggg gtagttatgg caaagtttat aacaatccgg gagtttcttg aaaaattcag
     5461 taatggcact aaatctttta tatttatata agctgttggc cttattcaaa acgaaggtgg
     5521 cattatttgt tgctgaagta atcaccgcca aagctgtatc ttgtgaaatt gtacttgata
     5581 accagttata accgccgtca ttagtctccc agatttcccc attattcgca ataacccatc
     5641 ccgtcaacga atcgagaaaa gaaatatcaa cggcataatc tactccatct tcaaaaattt
     5701 gtttacagaa aatccaggat tcaccataat cccttgtttt ttgtatttga ccgtcccgac
     5761 tcaataccca accataacca ttttcaataa aagaacattt gatataactc cgatcttcgt
     5821 tatgggtgat atgccaggtt ttccccgaat cggctgatac tgcaactcca cctctacctg
     5881 ttacgcaagc tttcccagcc gaaagaacta ccccataata aacggtatca ataaaaacat
     5941 tttccggatt aatggtcgca caattccagg tattcccgcc atctaaggtt atagcaatcg
     6001 tactttccgt tcctgttatg attccaaagg cagtgtctgc aaaagaaact gattgaagat
     6061 cggacaatcc catatatatg tcaaaatcaa acagggaatc ggcgactcta atccattctt
     6121 tgccgccatt agaagtttta aacaaacaat gtaacccagc ctcccaacca ttcaaactat
     6181 caacgaaatc aatattatta aaccaggaat attccggata tacgaactgc tccgaccagg
     6241 tttggcctcc atctttagta tttaatatta ctcctcctac tggtccgatt gtttctccgc
     6301 ctgcccaacc attcaatgaa tcagaaaatg ataatgtata aatccgataa ttttcaggaa
     6361 aaacaaaaat tctatcccac aattcgtttt gagcctctat tctctgagta aagaaaacaa
     6421 aataaatagt cagcattaaa atcaataata actttttcat tttacctcct tttcttttat
     6481 taaaacattt gttaattcac attgttagaa taatacggtt atcgcctgtt aaaataaaca
     6541 ttttcatatt actaaaactc cgaagatata tcaagtctat tttctataac tggaattctg
     6601 tcttgtaata aaaaaggata aaatcaaaac ggtgagggtc gtgtagaaat tacagcctca
     6661 ttaaaactaa tcaaatactt ccccatcttc gagtgtacgt aaaaagaatt attttacaat
     6721 tagttaatca acgactaata attcggctgt agtattnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnngcaa tatgatcgag atgaaaatgg ctataaaaaa cgcgcccgac
     6901 ttccagtacc gaacgctcag ccttgacaat ttgccgcaga gtacccgagc caccgtccag
     6961 caggagagtc tcatcgccaa tatctaacaa atagccggag caatttcgct tctcattcgg
     7021 taaaaacgag ccagagccca gaatcgttaa atgcatactt atcctccttt atcagtctac
     7081 agttttattt ccatcgctaa aatttgaaaa tatcaattat tgaaaacaat ataaataatt
     7141 tggtggtaat cgcaattatt ttatattttc cgccaatcaa catggagatt gaatgaataa
     7201 aaataacact ggggaaataa tccaggcaat gcctaaaatc gaactgcacc tgcatctgga
     7261 aggggctttc ccgctcgaat cccttttacg attaatccaa aaatacggca gtaacgaagt
     7321 gcataatata gaggaattgc gacagaaatt tgttttcact gatttcgctc attttatcga
     7381 aacctggtac tggaaaagtg atttttatcg tgagccgtcc gacattgaag atatggctta
     7441 ttctacaatt gcaagtttat ccctacaaaa cattatttac gctgaagtct atttttcacc
     7501 attcgatttt atcaagccgc aaatgccttt tccggtcgta actgaagccg taatttccgg
     7561 cgtacgacga gctgaatccg aatatccgat tcggattgga ttaattgccg acatcgttcg
     7621 taatcatggc cacgaaacag ctctagcgag attggataaa atcacgcaat accaaaatga
     7681 ggtcctcggc atcggactcg gtggcagtga aaaggaattt ccggctaaat tgttcgctga
     7741 ggtttatact gaggcccaaa aacgtggctt ccggttagtt gcccatgctg gtgaagccga
     7801 tggccctaat tccatctggg atgctttgcg ccttctgcat gccgaaagga ttggccacgg
     7861 cgtcagggcg gtcgaagatc ctgacctaat ggaatatttg cgattgaacc aaattccgct
     7921 tgaagtttgc gtcaccagca atctcaaaac aaaaatattc aattctgcgg ctgaacatcc
     7981 cgttcggcgc ttatttgatt atggcttact gataacgatt aactcagatg atccaactat
     8041 gtttggttca aacctaactg atgagtttaa ttttatcgcg aatgaatata attttacctt
     8101 acctgaaatt aagaagctga ctcaaaatgc gattcaagcn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnng attccctgct tttcacgcgt atcggctacg gcgagcagga
     8281 aattgtgctg ggaaatttat atgaaccggt aaaaatcatt ctccctttca ctccagtaaa
     8341 tctggattcg gtaaccgttg ccggacaaat ttttccacgt cagttgtccg gaattaactt
     8401 cagtcgagtt gaacgcactg aagacctcgg aaccgtcgag catcgccgct tatggacaac
     8461 aattcccggg ttatatctta aatcctatgg gggaccgact ggcataagca cattaagcct
     8521 ggacggttcg ccgtcaactc ataccagagt ggtaatcgcc ggttttgatt taaccagcgc
     8581 tcagaacggt cagatggaca tctcacaact gccaccgacc tttatatcga gcattaatta
     8641 ttctcccgaa caagccggca ataccagcgc tgaaaattca gaaggtttgt taaatatcga
     8701 acccaatatg tcgcctactg gttttacttg ctcagccggc agttacggca aattaaatct
     8761 gagcgccaat ctcgacattc gcaaaaccgt atggaattcc aacatattat taggtcacaa
     8821 ctattatcgc ggcgattatg aagttacctg gcgtgatcaa aatttcaagc gttcaaacaa
     8881 ttcgttacag caaaactatt tcagctggca gtctggatac gttttccgat cgaaggcatt
     8941 tgcgcgcctg atagcttttt actcagaaca ggagcgcgga gttgccggtc aagtctggaa
     9001 tccgtcgcca gatgcctttc ggcaagatgc gctgggattt atcggtctga aaattggctg
     9061 gacagttaag gccggacatt cttacattca gactttacat cgtcattcgg acgaacgcta
     9121 tgtcaatccg actattgcaa taaacagccg gcacgtcgtt aacaccgatc aaatcattta
     9181 ccatgccaat cggtcaatcc tcaattttaa cctcgatttt acttccgacc ttaaatccga
     9241 ccgattgcag agtaaacaag ctggtcatca ttcgcgtatt actttgacaa atattctagc
     9301 tttgccctgg aatttccacc ctaactatga actgcgtccc tgggttaaat tcgagcattc
     9361 tccggattta tatcaaaaag caacttgggg aggcagaatt gcatatcgtg gttctggaat
     9421 actcaatacc atctccggca gttatggcca atattacgcc tatccgacat tcaatgattt
     9481 atactggcaa ccgggcggta atcccaatct gaaaccagat gaaacaacta aatatgaaat
     9541 cgatctcatt ctccaaatcc ttccggagct taaacttgtc ttaaatagct attacaaaaa
     9601 ggataaaaac ctgattcaat ggacgccggt tcaatcctat tggcaacctt cgaatatttc
     9661 ccaagccgag cgaaaaggca tcaaagtcat cctcaactgg tcattaccca atttaccggt
     9721 tgacggcttc ttacagggca ttctaaccca ctcttgcaat ttgaccaaag gcaacaatta
     9781 ccaaaaacct ttgcgttata ctccgaagga atcttacgcc gctggtttaa attttaattt
     9841 caaacatatc gctttccgta gcacaattga atacgttggc gccagaattg cgatgtacaa
     9901 ctggccagaa gacataatcc tcgccgaata tctcgttctg aatgccaatt tagcttataa
     9961 aattgatacg acaatcggac gtttcaccat cgtcgctgga tgcgacaata tcaccgacga
    10021 atcattcgaa agtatgcaag gctacccaga acccggacgg gcttttaccg gaacaatcga
    10081 gtataaccca aaataaaaaa gagtggacta aaaaatgaaa cagaaactga ccttgttagc
    10141 cattataacg attttccaaa tctcactggc gcagaacttc acttacattc tatgcgaagg
    10201 aaattttaat acgactaatg cctcgctctg gcaattaacc ggcacgagtg agctaattgg
    10261 tcctgtccat tggaacttta ccgataatcc gctcggcgac gtcggccaaa acctaaaagt
    10321 ttatcaggaa aagctcttca tcgtcatgaa taattccaac tcgttggaag ttatgagttt
    10381 atcgaatggt gcaacttacg agcggacgat cgatttgccc tttgccggac cgcgtgatat
    10441 cgccttcgtg aacaatcacg cttacatcac ctgctggtat ttacctggca tactctcgct
    10501 ggatttaaat acctggcaaa ttgacgatac gatttttatc aatggcttac cagataatat
    10561 cattgcagat gatggaaaat tatatacctc aattgcgatg aataccgact ggtcgccggc
    10621 cgataaagtg ctcgctctgg aatcggtctc cggcgaattt acgcccgcgg atactttcac
    10681 tgtcatcacc ggtccgaatc agcttttatt atacgaaaat aagctctttg tcctcagtac
    10741 ttactatgac gatacctgga atacctatac gggaacttcc tgcattaatc tcgaaacaaa
    10801 tcaagtgacc aagcgggagc aggggctatc gttcaatttt tgtgacgacc ttgctctaat
    10861 aaacgataaa atatatcgca gttataacgg cggtgtggca ccgttggctg attcgcttac
    10921 gattgataca aataaaatca ttggcaatta tgctggtatc tattcaatgg ctttcgacgg
    10981 aaaaaatatt ttcatcggac tgagtgatta ttccgcacca gatcaggtgg tgattctcga
    11041 tacgctcggt aacgaaatta atactctaaa tgtaggcgtc tgccctggtt cttttgcttt
    11101 ttatcaacaa ccaaatgccg aaatatttac tgatgaaaaa aatggtattt tatctgattt
    11161 tttactgatg acaaattacc ctaatccatt caatagcaac acgattatcc ccttttacct
    11221 taagaacaca aatctcgtcc agctcacaat ctataatctc aaaggcgagc taatcgccaa
    11281 actgaccgat gattatttca c
//
LOCUS       DAOOMU010000076        10785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_6924_length_10785_cov_18.881454, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000076 DAOOMU010000000
VERSION     DAOOMU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10785
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6924_length_10785_cov_18.881454"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1218)
                     /locus_tag="PLI55_07715"
     CDS             complement(31..1218)
                     /locus_tag="PLI55_07715"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00761.1"
                     /translation="MVTFREDNEINCYIAMTGEHKIVTLLIVYFSLISLYSIPGNANE
                     WQEQKIQQALFEMYNLNNVAAHQHFKEVLSSQPFHPLAPMAELATDWLKNQEELGYQE
                     GNRILASRIDSVLAIYHDKLKANPQNGELEFYYGCTYGLKARLSLGEKDYLGVLINGY
                     NAVRYIKAAERHQPDFIELQIPFGVFNYYVGISRGYMQIASWIMNTSGSKEEGLAQMR
                     YAAHNARYSCYEARSILAMVTLYFEGDYESTLYYAEMMASDFPNNPYYNYLAGEALIQ
                     MGRYAEAEKQIKRIKQLLPKLKPKTQKEYWSKLHQLQGSLALKKGDLATAEKELRAVI
                     DNYDQEMDVQLGFALLRLGNVYDLTGRRELAVQCYRRAYGLKNRSQACQLAKLYLKTP
                     YTQ"
     gene            1274..1825
                     /locus_tag="PLI55_07720"
     CDS             1274..1825
                     /locus_tag="PLI55_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPB00762.1"
                     /translation="MLRDILDRRRATRSLAPVEITQAMIKDLAESARLMPSCFNKQPW
                     RFIFVYDKEQLTRLHPALNKGNEWAKKASLIVAVFTKKDLDCVIKDREYYLFDTGMAT
                     AALILRATEMGLVAHPIAGYDEDKVKEALNIPTEYQVIALINIGKQADEIDPILSDYQ
                     KLAEKERPARLPLENFVYLNKFS"
     gene            1869..3533
                     /locus_tag="PLI55_07725"
     CDS             1869..3533
                     /locus_tag="PLI55_07725"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010664793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPB00763.1"
                     /translation="MVNESSMASAGLVPIRQIAEKIGIPPEAMDLYGDYKAKIKLSYF
                     NQLPPNAKNKLILVSAMTPTPAGEGKTTVAISLAMGLNRIGKSAIVTLREPSLGPLFG
                     MKGGATGAGKAKVLPVEEINIHFTGDIHAVTAAHNLIAACLDNHIFRRKEPIIDPRRV
                     TLPRVMDMNDRALRNIIVGLGGKSNGIPRETGFIITAASEIMAILCLVKSYSEMQEKL
                     NRIILGYTYDSEPVCLGDLKITGAVASLLKEPLKPNLVQTSEGTPAFVHGGPFANIAQ
                     GTCSIMSMKMAAGLADFVVTEAGFGFDLGGEKFFDIVCRMNGLNPQAVVLVATVRALK
                     MHGGKSKSELDIPDLEAIRKGFENLEKHLENLAFFGIPGIVALNRFATDTEAEISLLN
                     ELVASKGYPFAVVDSFAKGSAGAIELAEKVAEIVQSERHYTPLYELNTPVIEKINTVC
                     QKIYGAEEVEYTPEARADLQQIKNLGLENLPICIAKTQNSLSDNPNLIGRPRNYTLTV
                     RQIGISAGAGFLVPVTGNILLMPGLPTKPVAENVTIDDDGNISGLF"
     gene            3751..5424
                     /locus_tag="PLI55_07730"
     CDS             3751..5424
                     /locus_tag="PLI55_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPB00764.1"
                     /translation="MPTSDKLKAQRQKWEETTLKKVISRNPERKTEFTTGSWLPVERT
                     ALPEITSDDEYLEKLGFPGEFPFTRGVQPTMYRGRLWTMRQYAGFGTAEESNRRYKFL
                     LNQGQTGLSIAFDLPTQIGYDSDDPHSEGEVGKVGVAIDTLRDMEILFDGIPLDQVST
                     SMTINAPAAILLAMYLAVAEKQGVAFDQLNGTIQNDILKEYIARGTYIFPPKPSLRLI
                     TNIFEFCARSVPKWNTISISGYHIREAGATAVQEVAFTLADGVEYVKAALNAGMDVDS
                     FADRLSFFFNAHNDLLEEVAKFRAARRIWAKIMRDKFHAQNSKSLMLRFHTQTAGSTL
                     TAQQPDNNIVRVAIQTLAAVLGGTQSLHTNSRDEALALPSEDSVRIALRTQQIVAYES
                     GVTNSVDPLGGSYLIENLTDQIESEVWQYLDQIEAIGGMIPAIENGFIQKEIQDAAYR
                     YQKEIESNERIIVGVNKFTTDEETPKNLLKVDSELQRIQIQKTDAVKKQRNNQAVKEK
                     LELLRETARRTEQNLMPPILAAVKEYATLGEICGILRQEFGEYQESIVL"
     gene            5434..5838
                     /locus_tag="PLI55_07735"
     CDS             5434..5838
                     /locus_tag="PLI55_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPB00765.1"
                     /translation="MAKKIRILIAKPGLDGHDRGAKYIARALKDAGYEVIYTGIRQTP
                     EAIVKTAIQEDVDFVGLSLLSGAHNELFPRVVELLRENQADDIIVFGGGVIPESDIPG
                     LKAKGLAAIFTPGTPIKAVIDFIESCRSKIEN"
     gene            5844..6785
                     /gene="meaB"
                     /locus_tag="PLI55_07740"
     CDS             5844..6785
                     /gene="meaB"
                     /locus_tag="PLI55_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPB00766.1"
                     /translation="MAEKILKGDQRSIGRAISIVENDGRDREQLIDEIFPHTNRAVVW
                     GITGAPGSGKSTLLDGLIANLRAQNRTVAVVAVDPSSPFTGGALLGDRLRMQRHATDL
                     GVFIRSMASRGHLGGIATATADTIKILDAAGYDLIVIETIGVGQAEIEVVELADIVML
                     VLVPGAGDEIQAMKAGVMEIGDIFIINKKDLDGALKLKAEVEYVLHLKSNSNESAINP
                     VLLTSAIHNEGISETLQTIDNYLARLQADGRLATRRQQRIENEVRKIVNTKIHQMAEK
                     HLQIDQKIKQWAQQIGKQKQAPYALINSQINQLLKDS"
     gene            6790..7194
                     /gene="mce"
                     /locus_tag="PLI55_07745"
     CDS             6790..7194
                     /gene="mce"
                     /locus_tag="PLI55_07745"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPB00767.1"
                     /translation="MIKKLNHIGIAVNSLAEHIPFYRDILQLNFLGIEEIADQKVKTA
                     IFQVGEIRIELLEATAPDSPIAQFLERRGEGLHHLAYQSDNIERELQFLREHNVALID
                     EKPRDGAHHTRIAFLHPKSTGKVLTEICEEKR"
     gene            7249..>7684
                     /locus_tag="PLI55_07750"
     CDS             7249..>7684
                     /locus_tag="PLI55_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HPB00768.1"
                     /translation="MDNKIHEELYKDKELTSTIIGCAYKVYKNLGFGFLESVYEKALI
                     IEMTKAGLKSESQKAIDVYYDDQIVGHFIADLFVEGKIIVELKSVATLTKSHEVQLVN
                     YLTATRINIGLLINFGEEKVEIKRKIRSLKQSNQSCKSCQKKK"
     assembly_gap    7685..7784
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7867..9417
                     /locus_tag="PLI55_07755"
     CDS             7867..9417
                     /locus_tag="PLI55_07755"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPB00769.1"
                     /translation="MSRQRKLLELKDKIELARMGGGAEAIEKQHQRGKLTARERLQLL
                     LDPNSFEEIDAFRTHSCTDFGMADKKFLGDGVVTGYGTINGRLVFVYSFDFTVFGGSL
                     SKVVAEKIVKIMEMATKVGAPLIGINDSGGARIQEGVDSLAGYTEIFVRNVLYSGVIP
                     QISAIVGPCAGGAVYSPALTDFIFMVRKSAYMFLTGPKIVKTVTHEDVTADELGGADV
                     HASKSGITHFTYDDEQSLFAGMRELLSYLPQNNMEEPPAIAPTDPVDRIDEDLNYIVP
                     ENPNKPYDMKEILAKVLDDGQFLEVQALYATNMLIGFGRLNGKVVGIVANQPRSLAGV
                     IDTNASNKAARFVRTCDCFNIPIITFEDVPGFMPGTAQEFNGIIKHGAKLLYAFAEAT
                     VPKITVITRKGYGGAFCVMNSRQLRGDYSFAWPSAEIAVMGPEAAAEIIFAHEIKTVE
                     NPEQRFAEKVEEYREKFANPYIAASKGYIDEVIEPKVTRYKLIKTLEMLSNKRDTLPP
                     KKHGNIPL"
     gene            9427..9780
                     /locus_tag="PLI55_07760"
     CDS             9427..9780
                     /locus_tag="PLI55_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPB00770.1"
                     /translation="MNPELLNGIIIGIGGLIIVFVGLLIIEFVIALFSQYFAKKEQKT
                     QTAAGLPLIETKKHSAQTKRIAIPEEDVVAIATTIELYRKIHFEQLPSQITFIRGNQQ
                     SNWKIGSIASQFLRK"
     gene            9782..10258
                     /locus_tag="PLI55_07765"
     CDS             9782..10258
                     /locus_tag="PLI55_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPB00771.1"
                     /translation="MKEKKVELEINGQPYTVIINAISAYHASVTVNDRTYEVGLKDLG
                     FDQVADIKPKLINVPESAVPTAKPASKTPPTQLHRPKSVIDSSAVVAPLPGLIQNILV
                     KVGDTVHAGQHVLTMEAMKMENEIQTNRDGIIKDIKVRIGDSVNEGDTLIILNSGE"
     gene            10262..>10785
                     /locus_tag="PLI55_07770"
     CDS             10262..>10785
                     /locus_tag="PLI55_07770"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPB00772.1"
                     /translation="MDSFLKILQTTGFANIQIGNIIMIFIGLIAIYFAITKKYEPLLL
                     LPIGFGIIVGNIPSIPSIAIGVFDTVENGAASNSVFSMIYYLVTSGIFPPLIFLGIGA
                     LTDFSSLISNPKSLLLGAAAQLGIFITFIGALVLGFSAPEAASIGIIGGADGPTSIFL
                     ASQLAPHLLGPIAIA"
BASE COUNT         3080 a         2499 c         2333 g         2773 t
ORIGIN      
        1 ctcagcctct ctttctcagt ctcagccttt ttattgagta tagggtgttt tcaaataaag
       61 tttggctaat tggcaggctt gcgagcggtt cttcaatcca tacgctcgcc ggtagcattg
      121 aaccgccaat tcccgccgac cagtcaagtc atagacattg cccaatctaa gaagcgcaaa
      181 tcctaactgg acatccattt cctggtcata attatcaatc actgcccgaa gttccttctc
      241 agctgtcgcc agatcacctt tcttaagcgc cagactgccc tgcaattggt gcaattttga
      301 ccaatactcc ttttgcgtct tgggctttaa tttcggcaat aattgtttga ttcgcttaat
      361 ctgtttttca gcctcggcgt agcgccccat ttgaatcagc gcctcaccag ctaggtaatt
      421 ataatacgga ttattgggaa agtcggacgc catcatctcc gcatagtaga gtgtggactc
      481 gtagtcacct tcgaaatata aagttaccat tgccagaata ctgcgcgctt cgtaacaact
      541 gtaacgcgca ttatgagcgg cataacgcat ctgcgccaat ccctcctctt tacttcccga
      601 cgtattcata atccaggagg cgatttgcat atagcctcgc gaaataccca cataataatt
      661 gaacacgcca aaaggtattt ggagctcaat gaaatccggt tgatgacgtt ccgccgcctt
      721 gatatagcgt accgcattgt agccgttaat caagacaccc aggtaatcct tttcaccgag
      781 cgacaaccgg gctttcaaac cgtaagtaca accataataa aattccagct cgccgttctg
      841 cgggttagct tttaatttat catgataaat tgccagaacg gagtcaatcc gcgaagccag
      901 gattcgattg ccctcctgat agcccaattc ttcctgattc ttcaaccaat cagttgccag
      961 ttctgccatc ggcgccaacg gatgaaacgg ttggctactt aatacttctt taaaatgctg
     1021 gtgcgcggcg acgttattca ggttatacat ctcaaataag gcttgctgga ttttttgttc
     1081 ctgccattcg ttggcattac ccggtattga gtacaaactg attaaagaaa aataaacaat
     1141 taagagcgtt acaattttat gttcaccagt cattgctatg taacaattta tttcattatc
     1201 ttctctaaag gtaaccattg aaaggcgtgc aaaacaagtg aaaatgcaaa agattaaaat
     1261 agggaggttt gttatgctga gagacatttt agacagacgc cgtgccacgc gctcattagc
     1321 gccagttgaa atcacccagg cgatgataaa ggatttggcg gaaagtgccc ggttaatgcc
     1381 gtcttgtttt aataaacagc cttggcgatt catttttgtt tatgataaag agcaattgac
     1441 ccgcctgcat ccagctctta acaaaggcaa tgaatgggct aaaaaggctt cgctaatagt
     1501 ggcggttttc actaaaaaag acctcgactg cgttatcaaa gatcgagaat attatctatt
     1561 cgacaccgga atggcgactg cggctctcat cctgcgcgca accgaaatgg gattggtcgc
     1621 ccatcccatt gccggttacg atgaggataa agtcaaggag gctttgaata ttcccactga
     1681 ataccaggta attgctctca taaatatcgg taaacaagct gatgaaattg atccaatttt
     1741 aagcgattat caaaaattag ccgaaaaaga acgaccggcg cgcttgcctc tcgaaaattt
     1801 tgtttattta aataaattta gttaacatat agccgttgat taaattaatt aactaatata
     1861 ggcgcgttat ggtcaatgaa tcttcgatgg cttcagcagg tcttgttcca attcgccaaa
     1921 ttgcggaaaa aattggaatc cctcccgaag caatggattt atatggtgat tacaaagcca
     1981 aaatcaagct gagttacttc aatcagctcc ctcccaacgc taaaaataaa ttaattctgg
     2041 tctcggctat gacgccaacc ccggctggtg aaggtaagac aaccgtagca atcagtctgg
     2101 caatgggact gaaccgcatt ggtaaaagcg cgattgttac attaagagaa ccatcgctgg
     2161 gtccgctttt tggtatgaag ggcggcgcga ccggcgctgg caaagccaaa gtacttccag
     2221 tcgaagagat caatattcat tttaccggtg atatacatgc cgtaactgcc gctcacaact
     2281 tgattgccgc ttgtctcgat aatcacattt tccgtcgcaa ggagcctata attgatccaa
     2341 gacgggttac cttgccacgg gttatggata tgaatgatcg ggctttacga aacatcatcg
     2401 tcggtttggg cggcaaatct aatggaattc ctcgtgagac gggattcatt ataaccgccg
     2461 cttctgaaat tatggcgatt ctctgcttgg tgaaatcgta ttccgaaatg caagaaaaac
     2521 ttaatcgtat aatcctgggc tatacttacg acagcgagcc ggtgtgttta ggcgatttaa
     2581 aaattaccgg agcggttgca tctttattaa aagagccctt gaaacccaac ctggtacaga
     2641 ccagcgaagg cactccggct tttgtgcatg gtggaccgtt tgccaatatt gcgcagggaa
     2701 cttgctcgat aatgtctatg aagatggcgg ctggattggc agatttcgtc gttacagaag
     2761 ccggttttgg gttcgatctc ggcggcgaga aatttttcga tattgtctgc cgaatgaacg
     2821 gtcttaatcc acaggcagtc gtgttggtgg ctacggttcg ggcactcaag atgcacggcg
     2881 gtaaatctaa atccgagctg gatattcctg accttgaagc cattcgaaaa ggttttgaaa
     2941 atttagaaaa gcatctcgag aatctcgctt tcttcggcat cccgggaatt gtggctttaa
     3001 accgatttgc aactgatact gaggcagaaa ttagtctatt aaatgaatta gttgcctcca
     3061 aaggataccc attcgctgtc gtggattcat tcgcaaaagg aagcgccggg gcgattgagc
     3121 tggctgaaaa agtcgccgaa attgttcaaa gcgagcggca ttatacgcct ttatacgagc
     3181 tgaatacccc agtgattgaa aaaatcaata cagtttgcca aaagatttat ggtgcagaag
     3241 aagtcgaata caccccggaa gctcgcgccg atttgcaaca aattaaaaac cttggcctcg
     3301 agaatctgcc tatctgtatc gccaaaaccc agaacagcct ctctgataat ccaaacttga
     3361 tcgggagacc gcgcaattat actctaaccg taaggcagat cggcatttcc gccggagcgg
     3421 gttttttagt gcctgtgact ggcaatatac tgttgatgcc cggcttgcca actaaaccag
     3481 tcgccgagaa cgtcaccatt gatgacgacg gtaatatcag cggactgttt taatcgaact
     3541 acaatatctt tagcttttaa tttctcgctt taatagccca aataatttac ccaacttcca
     3601 atcttgctag ccatcttgtt tttctcctga ttcctcttta attttttaag tcattttgac
     3661 agcgagatgt aacgttaatg tcgctcacaa tacaatcatt tcttctccat accataacca
     3721 gtatccataa taattaatca ggagcaatct atgccaacct ccgataaatt aaaagcacag
     3781 cgccaaaagt gggaggagac tactctaaaa aaagtcatct ctcgcaatcc tgaaagaaaa
     3841 acggaattca ccaccggctc atggttaccg gtcgaacgca cggctctgcc ggaaattact
     3901 tccgacgatg aatatctgga aaaactcggc ttccccggtg aatttccctt cacgcgcggc
     3961 gtccagccta cgatgtaccg cggtcgtctc tggactatgc gacagtacgc cggctttgga
     4021 actgccgaag aatctaaccg gcgttataaa tttctgctta atcagggaca aaccggctta
     4081 tcaatcgcct tcgatttgcc gactcaaatc ggctatgatt ccgacgatcc gcatagcgaa
     4141 ggcgaagtcg gtaaagtcgg cgtagcgatt gatactctgc gcgatatgga gatacttttc
     4201 gatggcatac ctttagacca agtttccact tcgatgacaa ttaatgctcc cgccgcaatt
     4261 ttactggcaa tgtacttagc agtagccgaa aaacaaggtg tcgccttcga ccagctcaat
     4321 ggcacaatcc agaacgacat tctgaaagaa tacatagccc gcggcaccta tatttttcca
     4381 cccaagccgt ctctacgttt aattactaat atttttgaat tttgcgctcg ttcagtcccg
     4441 aaatggaata cgattagcat ttccggctat catattcgcg aagccggcgc gactgccgtc
     4501 caggaagtgg ctttcactct ggctgatggc gttgaatacg tcaaagccgc tttgaacgcc
     4561 ggaatggacg ttgatagttt tgccgaccgg ctctcctttt tctttaatgc ccacaacgat
     4621 ttgttggagg aagtcgccaa attccgcgcg gcacggcgca tctgggctaa aattatgcgc
     4681 gataaattcc acgcccaaaa ctcgaagtct ctaatgttgc gctttcatac tcaaacggcc
     4741 ggtagtactc tcaccgccca acagccggac aataatatcg tccgggttgc aatccaaaca
     4801 ttagcggcag tgcttggcgg cacccagagt ctgcatacca attcccgcga tgaggctctg
     4861 gctttaccgt ccgaagattc cgtgcgcatt gctctacgca ctcagcaaat tgtcgcctac
     4921 gaatccggcg tgactaactc agttgatccg ctgggcggct cctatttgat tgagaatctt
     4981 accgatcaga tcgaaagcga agtctggcaa tatctcgatc aaattgaagc cattggcggg
     5041 atgattccgg ctattgaaaa tggctttatc cagaaagaaa tccaggatgc ggcttaccgc
     5101 taccaaaaag aaatcgaaag caacgagcgc atcatcgtcg gcgtcaataa attcaccacc
     5161 gacgaagaaa caccgaaaaa tttattaaaa gttgattccg aacttcagcg catccaaatc
     5221 caaaaaactg acgccgtcaa aaaacaacgc aacaatcaag cagttaagga aaaacttgaa
     5281 cttttacgag aaactgcgcg ccgaacggag cagaatctta tgcctcccat tctcgccgcc
     5341 gttaaggaat atgccacact gggcgaaatc tgtggcattc tgcgccagga attcggcgaa
     5401 taccaggaat caatcgtttt ataaggattc aatatggcta aaaagatcag aatattaatt
     5461 gccaaacccg gactggatgg acacgaccgc ggcgccaagt acattgcccg cgctctgaaa
     5521 gatgccggat atgaggtaat ctacaccgga attcgtcaga caccagaagc cattgtcaaa
     5581 actgctattc aggaagatgt tgacttcgtc ggtctcagcc tgttgtccgg ggctcacaat
     5641 gagcttttcc cgcgagttgt cgaactttta cgtgaaaatc aggctgacga catcattgtt
     5701 ttcggtggtg gcgttatccc cgaaagcgac atccccggtt taaaggcaaa aggattagcc
     5761 gccattttta cacccgggac gccaattaaa gctgtcatag attttattga atcctgccgg
     5821 agcaaaatcg aaaattgagc gaattggctg aaaaaatttt gaaaggcgac cagcggagca
     5881 tcggtcgtgc catttctatt gtagaaaatg acggcagaga ccgcgaacaa ttaattgacg
     5941 aaattttccc gcacaccaat cgggcagtag tttggggaat cactggcgca cccggcagtg
     6001 gaaaaagcac tttgctggat ggtcttatcg ccaatttgcg ggctcaaaac cggacggtgg
     6061 cggtagtcgc ggttgatccc agttcaccgt ttactggcgg tgcgcttctc ggtgatcgtt
     6121 tacgcatgca aagacatgcc accgatttag gcgttttcat ccgctcgatg gcttcgcgcg
     6181 gacatttagg tggcattgcc actgctacgg ctgacactat taaaattttg gatgccgccg
     6241 gctatgattt aatcgtgatt gagactattg gtgtcgggca agctgaaatc gaagtcgtcg
     6301 aactggctga tatcgttatg ctcgttctgg taccgggagc gggtgatgaa attcaggcga
     6361 tgaaagccgg agtcatggaa attggtgata ttttcattat caataagaaa gacctcgacg
     6421 gcgctctcaa actcaaagcc gaagtcgagt atgtcctcca cctcaagtca aatagtaatg
     6481 aatccgcaat aaatccggtt ctattaactt cagcgattca caatgaaggc atatcggaaa
     6541 cactccaaac tattgacaat taccttgctc gattgcaggc tgacggacgg ctggcaactc
     6601 gccgtcaaca gcggatcgaa aacgaagtca gaaaaatcgt caacaccaaa atccaccaaa
     6661 tggctgaaaa acatctgcaa attgaccaaa aaatcaagca atgggctcag caaatcggaa
     6721 aacaaaagca agcgccttat gccttgatca attctcaaat taatcaatta ttaaaggact
     6781 cctaattcta tgataaaaaa actcaaccac atcggcatcg cggtcaattc tcttgcggaa
     6841 cacataccgt tttatcggga tattttgcaa ttaaatttcc ttggcatcga agaaattgcc
     6901 gaccagaaag tcaaaaccgc catctttcaa gtcggtgaaa tccggatcga attactcgaa
     6961 gccactgcgc ccgatagtcc aatcgcccaa tttctcgaga gaagaggtga aggtttgcat
     7021 catctggctt accaaagcga caacatcgaa cgagaactgc aatttttaag agaacataat
     7081 gttgccttga ttgacgaaaa gccgagagac ggcgcgcatc atactcgcat cgctttcctg
     7141 cacccgaaat ccactggcaa ggtcttgact gaaatctgtg aggaaaagag gtagttttag
     7201 acaagattaa caagatttac atgttgttta ttaatattta gaactaagat ggataataaa
     7261 atacacgaag agttatacaa ggataaagaa ttgacatcca ccataatcgg ttgtgcttac
     7321 aaggtctata aaaacctggg attcggcttt ttagagtccg tctatgaaaa ggctctgata
     7381 attgaaatga ccaaagcggg tttgaaatcc gaatctcaga aagcaattga tgtctattat
     7441 gatgaccaaa ttgttggaca ttttattgcc gatctttttg tagaaggaaa aataatcgtt
     7501 gaattaaaat ctgtagcgac tttgacgaaa agtcacgaag ttcaattagt gaattactta
     7561 acagctacca gaataaatat cggactatta ataaatttcg gagaggagaa agtcgaaatc
     7621 aaacgcaaaa taagatcatt aaaacaatca aatcaatcat gtaaatcttg tcaaaagaaa
     7681 aaaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaagaa aaaaaatcaa
     7801 tcttgttaat cttgtctaaa ataaaaacaa tgttaatctt gtcaataaaa tatacaagga
     7861 gcataaatga gtcgtcaacg taaactactt gaattaaaag ataaaattga acttgccaga
     7921 atgggcggcg gtgcggaagc catcgaaaaa cagcatcagc gcggcaagtt aacggcccgg
     7981 gagcgcctgc aattactact cgatccgaat agttttgagg aaatagatgc ctttcgcact
     8041 cattcctgca ccgacttcgg tatggctgac aagaaatttc tcggcgacgg agtcgtcacc
     8101 ggttatggta ctatcaatgg tcgtctggta ttcgtttatt cttttgattt cacggtcttc
     8161 ggtggttcgc tatcaaaagt cgtagctgaa aaaattgtca aaattatgga aatggcgact
     8221 aaagtcggcg ctccgttaat cggcatcaat gattccggag gcgcgcgaat tcaggaaggg
     8281 gtggattcac tggccggtta taccgaaatt tttgtgcgca atgtgcttta ctcgggcgtc
     8341 attccccaga tcagcgccat cgtcggacct tgcgccggcg gagcagttta ttcaccagct
     8401 ttgaccgatt ttatttttat ggttagaaaa tcagcctata tgtttttaac tggcccaaaa
     8461 atagtcaaaa ccgttactca cgaggatgtc accgccgatg aactgggtgg cgccgatgtt
     8521 cacgccagca aaagcggcat tactcacttt acttatgacg atgaacaaag tctgttcgcc
     8581 ggtatgcgcg aactgctgag ctatttaccg caaaacaata tggaagaacc gccggctatt
     8641 gcaccgactg atccggttga tcgcattgat gaggacttga attatatcgt gcccgaaaat
     8701 cccaataagc cttacgatat gaaggaaatt ctcgccaaag ttctggatga cggacaattc
     8761 ctcgaagttc aggctttgta cgccaccaat atgctaatcg ggttcggacg tttaaacggc
     8821 aaagtcgtcg gcattgtcgc caaccaaccc cgatcgctgg cgggcgtcat tgacactaac
     8881 gcgtcgaaca aagccgcccg tttcgtgcgc acctgcgatt gtttcaatat tcccatcatt
     8941 actttcgaag atgtgccggg atttatgccg ggcaccgccc aagaattcaa cggcattatc
     9001 aagcacggcg ctaaactcct ctatgctttt gccgaggcaa ccgtgcccaa aatcacggtc
     9061 attacccgca agggctatgg cggcgcattt tgcgtcatga attcgcgcca actgcgcggc
     9121 gattacagct tcgcctggcc cagtgccgaa atagccgtaa tgggtccaga agccgccgcc
     9181 gaaattatct ttgcgcacga aattaaaacc gtcgaaaatc cggaacagcg tttcgccgaa
     9241 aaagtcgaag aatatcgcga aaagttcgcc aatccctaca tcgccgcctc caaaggctac
     9301 attgatgaag tgattgaacc taaagttact cgctacaaat tgatcaaaac gctggagatg
     9361 ctttctaata agcgcgatac tctcccacct aagaaacatg gcaatattcc tttataggag
     9421 caagcaatga acccggaact attgaatgga atcataatcg gaatcggtgg actgattatc
     9481 gtatttgtcg gattgttaat tatcgaattc gttatcgcac tgttcagcca gtattttgca
     9541 aaaaaagaac agaagaccca aacggctgcc ggtttgccat taatcgaaac taaaaaacat
     9601 tcggcgcaaa ccaaacgcat tgccatcccg gaagaagatg tggtcgccat cgctacgaca
     9661 attgaacttt atcgcaaaat ccacttcgag cagttaccaa gccagattac ctttatacgt
     9721 ggcaatcagc agagcaattg gaaaatcggc tcaatcgcca gtcaattctt gaggaaatag
     9781 tatgaaagag aaaaaggtag aattagagat aaacggtcaa ccttacactg tcatcatcaa
     9841 tgcaatttct gcttaccacg cttccgtaac cgtcaatgat cgcacttacg aagttggctt
     9901 aaaagatctg ggcttcgatc aagttgctga cattaaacct aaattaatta atgttcctga
     9961 atcagccgta ccaaccgcta aaccggcaag taagactccg cctactcaac tgcatcggcc
    10021 taagtcggtg atcgacagct ccgcggtggt agcacccttg ccgggtctga ttcaaaatat
    10081 attagttaaa gtcggggata ccgtccacgc cggccagcac gtcctgacga tggaagccat
    10141 gaaaatggaa aacgaaatcc agactaaccg tgacgggatt atcaaagaca ttaaagtccg
    10201 aatcggcgat tcggtcaacg aaggcgatac cctcataatt ctcaattcag gggagtgata
    10261 aatggatagt ttcctgaaaa ttctacaaac gactggcttt gctaatattc agatcggcaa
    10321 tattattatg atttttatcg ggctgattgc catttacttt gcgataacga aaaagtacga
    10381 accgctcctg cttttgccga ttgggttcgg tataattgtc ggcaatattc cttccatacc
    10441 atctattgca attggagtct tcgatacagt tgagaatggc gctgcgagta acagcgtttt
    10501 cagtatgatt tattatttag tgacttcggg aatatttcca ccgctgattt ttctgggcat
    10561 cggagcctta accgatttct catcattaat ttctaatccg aaatcacttc tactgggagc
    10621 cgccgctcag ttaggtattt tcattacttt tatcggcgcg ttggttctcg gtttttctgc
    10681 gccggaagcc gcctcaatcg gcatcattgg aggagcagat ggaccaactt cgatctttct
    10741 cgcctcccaa ctcgcgcccc atttactggg accaatcgca atcgc
//
LOCUS       DAOOMU010000077        10696 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_7007_length_10696_cov_13.201109, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000077 DAOOMU010000000
VERSION     DAOOMU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7007_length_10696_cov_13.201109"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..2125)
                     /locus_tag="PLI55_07775"
     CDS             complement(218..2125)
                     /locus_tag="PLI55_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB00773.1"
                     /translation="MKRMLVFLLCAIFLTVGLNAQEKAVVEVNGIYGGGLEGSLNDAV
                     KTVLNTEGTLSNTVFKLKSNDWYVMTGVIEVPVGQVLELVGEDIGATQETSPPQMLWT
                     ASTSVNKTFMIAVYGDLIMKNIWVRFVDVAGVQTGTPIVFDGDTTGQSPDDKEEGYFE
                     NCIFEYMPCPVVTSSGSICIRCKHFNGTFKNCYFRNCVDRHFMYYGRAVSYPFDVPGY
                     HTDYISFENCTFANMGYVLMQEGSNWGENVHFNHCTFYNVVMFSLESGWWWKLNVTNS
                     LFVNAYMLGYIPLSGAGSATISIEPADSIDFAIPFTDQNRHILFAHSAYYIDDWLVDW
                     MHGGWELPFADDPDSVERWRPPSRITQVGSPYSRSLYKERAFNEIPYPRPMLDSTALM
                     YFDSTDSEGKKVYPYINRAALYSVEDLRNAVNPRLIVPPLNLEPLKYFLNEKWGTNLD
                     TMWAYEPLIGHEIQCWPLPENLAYANDTLKTAGMGGFPLGDLYNWWNPTVREGATDYY
                     SAWLAQADDERAKIAQWLETGVHPDSAQNAIKPQPKTSGPSEFSLAQNYPNPFNPTTQ
                     IKYTVPYDARVSLKVYNTLGQEVANLVDGTQSAGEHIVTFDGKNLAGGIYFYTLKSEG
                     VNITKKLVLIK"
     gene            complement(2203..5238)
                     /locus_tag="PLI55_07780"
     CDS             complement(2203..5238)
                     /locus_tag="PLI55_07780"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPB00774.1"
                     /translation="MKISVRFIKMTRGRGCKAFFIAGLVLCLMTQSLWAKGTIRGRVF
                     DKNTKDPLSYANIMIKGTTIGTAADADGMYSITNAPDGPQTIVISYIGYVTLEVDVNI
                     PDGGTVRRDFGLEPEVLQGKEVIVTAQALGQMQAINQQLASDKIASVVSEARIQELPD
                     FNAAQALSRLPGVSTLESSGEANKVVIRGLAPQYNAVAVEGIKLASTGSTQIGVSALG
                     NTAGSISNDRSVDLSMISPYMIKSISVFKSLTPDMNANSIGGSVNMELREAPSQLHYD
                     LMWQSGYTAKTNNYGNYRAVGSISQRFFQDNLGAYLLINAEKYDRDADNMSANYRVTS
                     SVIDTSTGYRPVSVNSVTLNRHVETRKRYGGNLILDYKLPAGSIKSVNMYTRFVSDYQ
                     DYNTVLNYFDKILNFNYRGGINTTDLSVNSLELKYDFRYIQMDLKAANTHSRNDLPES
                     PYITFKTGQAMMGSVPINTVPDSLTRLWVYPGHDKVYLDYLNLFSSLYTENNDNFSSD
                     FKVPFSIGKSLLGYFKFGGQYYNQKNTNDQKTPYAEIRKSGEFQAAMVEDLERQFGIP
                     VDPSTAKFAASNFTGSSKLMQPFLDNQFGEIYWACKPDILVEMADYLNNNPQWRGKAT
                     GGADQTGGWYNGMFQNLANSYRYREEYYAGYVMSEIDFRNLMIVGGARYEKTESKYTA
                     YNMYDMRNPDAQNCDTVYSYPKSEFWLPMVQAKYSPFEWFDIRYAYTQTLARPDYHQM
                     SPKINYDNTRRSVRAGNPDLVPAHAYNHDLNLTFHGNKLGLLSIGGFYKTIKDFVYST
                     AYILHKTSKSSDIKTINDIQIMGIKPEEGATLYTYINSRYLAYVKGLEFDWQTRFWYL
                     PWGLDGIVLGVNYTMIESEATYPLREEVTDYTVRPPITTTRDSTRTGRLLYQPNDVVN
                     AYIGYEYKGFSARLSFLFQGNSVSYVGTFPEQDGYTRDYFRIDASVRQTLPWLGSEIY
                     LDVNNLNNEVNSSAQMSIDGFTNVKHYGMTANLGIRFRL"
     assembly_gap    5360..5459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5460..6416
                     /locus_tag="PLI55_07785"
     CDS             <5460..6416
                     /locus_tag="PLI55_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPB00775.1"
                     /translation="FFFLKASEINIILGLEVINMDRRKFIKTSAFLTGGMVLRGAQIL
                     GKETTIPDLVMVKDGQPAEMAQKTFELLGGVPRFVAKGDYVLLKPNLSWDRAPEYAAT
                     TNPELVAEIVRLCFQAGAKKVVILDNTCADARRSYDACQIPVYARQAGADVRFVRENQ
                     FVETPIPNGTNLKSWPIHQEVFNADKLISIPILKHHGIPGITSGFKNMMGLLGGQRGR
                     IHRPFSENIVDINRVIKPNLTIIDAIRVLRRNGPQGGNLNDVEQMNILLAGTDRVLVD
                     AWAAKIFGLNPLTVEYLKLAHSAGLGEIDTEKFKPVVYSFKA"
     gene            6413..7948
                     /locus_tag="PLI55_07790"
     CDS             6413..7948
                     /locus_tag="PLI55_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB00776.1"
                     /translation="MKTKSLTWRKISQVFFFLLFTLTLLQATYPFKPWLPPELFLWFD
                     PLAAITTQIAGRFFDIVFLLAAIILISPLIFGRAFCGWICPMGTLIDVSDHILKPKKR
                     RDFRHLRFLKLAILTAVIVLAIFGVQYAWLLDPLPVAWRSIGVIGLAFFFLLSDSILS
                     GLISIGIFPDALLSFQDKIAGFLFPVASPNFGGLVAPLIIFLIILGLSRLSRRFWCRY
                     LCPLGALLGLVARISPLRRIVDADKCSACGICRHKCKMDAIEPDFVTTTKSECILCLN
                     CKYDCATEAISFGWRAPTQMMNKVDLSRRAFIGTSLTALAGVGFLKINKVNPRHSDRL
                     IRPPGALNEDDFLERCIRCGECVRICSTSGKCLQFSVLESGLEGFMTPLAEYRRGYCE
                     YNCNLCGQICPTKAIQPLNVSAKQKAKMGQAIFLKDRCIPYRLNENCIVCEEHCPIPD
                     KAIKINTREYEDPVTGRKRMVSYPYVDEALCIGCGICENKCPLNGEAGIIVIREGEER
                     SRA"
     gene            8241..9368
                     /gene="amrS"
                     /locus_tag="PLI55_07795"
     CDS             8241..9368
                     /gene="amrS"
                     /locus_tag="PLI55_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPB00777.1"
                     /translation="MAITRRQFCLKLGSGVAASVFVPRLIKSGWTKPATDSKPYLREA
                     RYYTPLADRKVRCLLCPRTCLVADGKRGYCRVRENQKGSYKTLVYGRLCSINNDPIEK
                     KPLFHFLPGTMALSVATAGCNLKCKFCQNWNISQVAPEDIPFEYYSPERLVNLAQQIQ
                     SPTIAFTYTEPTISTEYILDTAKLARSKGIHSVMISNGYINSEPLGDLCQVLSAIKID
                     FKAFSDDFYRNVTDGTLQPVLDTMIQIKKNNVWLEIVNLVIPTQNDAPAQFRQMSQWI
                     LQNLGSDIPVHFTRFHPLYLMENLPPTPLRTLESAYEIARECGLRFVYVGNVPGHAAE
                     NTYCPHCNRKIIERIGYQIRTFAIKNGKCQYCQTNIPGFWS"
     assembly_gap    9442..9541
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9542..10681
                     /gene="amrA"
                     /locus_tag="PLI55_07800"
     CDS             <9542..10681
                     /gene="amrA"
                     /locus_tag="PLI55_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPB00778.1"
                     /translation="VDKKLAAQCVTADRLVKLATEGHLSQRFGRAEHSLEVQLPFLQI
                     VLPNTPIVPIVIGTHDWRVLESLGNRLGELLVQKNILIVASSDFSHYHSYSECQKIDQ
                     RAIESLKQLDAVKFYQGFTSETFEACGGAPITALLIAAQKMKNPQIEILKSANSGDVP
                     HGDKSAVVGYVAVGLYQGTKAKSQDDNTASSQLNTGDLTREEQLYLLDLAEKTVAAAV
                     NHRPLPDPKDVPERLKVKRGAFVTLEKHHELRGCIGYILPIYPLYKTVIEMAKAAALD
                     DPRFPPVSPSELKDITVEISVLTVPQKISDPSEIEVGKHGIIIKRGFNQGLLLPQVAT
                     EYGWDRETFLEHTCLKAGLPRDAWQDKNTEIQIFSAQVFNRETVK"
BASE COUNT         2980 a         2432 c         2132 g         2952 t
ORIGIN      
        1 tgaactgtca cgcctcctaa tagcaggaac acaaccgtaa tggcaaaaaa agtatttact
       61 acctttttca tagtttcatt tctcctttct ttttttttca taacaaaaaa atcttccatc
      121 atttctacaa acactatgta gtagaatgtt tccttttgaa tcacctcctt ttttaatcga
      181 gttcatattt tctattcttc agctttaaat ataaacatca tttaatcaag actaactttt
      241 tggtaatatt gacgccttcc gactttaaag tgtagaaata gatgcctccg gctagatttt
      301 taccatcaaa agtcacaata tgctcgccag cggattgagt gccatcaacc aaattagcta
      361 cttcctgtcc gagagtattg taaactttta acgaaacccg ggcatcataa ggaacagtat
      421 atttaatctg agtagtcgga ttgaaagggt tcggataatt ctgggcaagt gaaaattctg
      481 aagggccact cgttttcggc tgaggcttga tcgcattctg agcgctatca ggatgaactc
      541 cggtttccaa ccattgcgca attttggctc tttcatcatc ggcttgtgcc aaccaggctg
      601 aatagtaatc ggtagcgcct tctctaaccg tcggattcca ccaattatac aaatcaccta
      661 acggaaaacc acccatccca gccgtcttta aagtatcatt cgcataggct aaattctccg
      721 gtaacggcca gcactgtatt tcgtgaccaa tgaggggctc gtaagcccac atcgtatcca
      781 gattagtgcc ccacttttca ttcaagaaat attttaacgg ttccaaatta agcggaggaa
      841 caattaaccg cggattgact gcatttctca agtcttcaac actataaagt gccgcccggt
      901 tgatataagg atagactttc ttaccttcac tatcagtgga atcgaaatac atcagtgcgg
      961 tgctatccag catcggccgc ggatagggaa tttcattaaa agcccgttct ttataaaggc
     1021 ttctactata tggactgcct acctgggtta ttctagatgg tggccgccat ctttcaaccg
     1081 aatcgggatc atctgcaaaa ggtaactccc atccgccatg catccagtcc actaaccagt
     1141 catcaatgta ataagcgcta tgagcgaaga gaatatgacg attttgatca gtaaagggta
     1201 tggcgaaatc aattgaatca gccggctcaa ttgatatagt cgcgctacca gcacctgaca
     1261 atggtatata gcccaacata taggcattga caaaaagact gttagttaca tttaatttcc
     1321 accaccaccc tgactccaga gaaaacatta caacattata gaaagtacag tggttaaagt
     1381 gaacattttc accccagttg ctaccttctt gcattaagac atatcccata ttagcgaatg
     1441 tgcagttttc aaaagatatg taatcggtat gataaccggg gacatcaaaa ggatacgaaa
     1501 ctgctcggcc ataatacata aagtggcgat ctacacagtt gcggaaatag cagtttttaa
     1561 aagtgccatt gaaatgttta catctaatgc agatcgaacc ggatgatgtt acaaccggac
     1621 aaggcatata ttcaaaaatg caattctcaa aataaccctc ttctttatca tccggcgact
     1681 gtccggtggt atctccatcg aatacgatag gggtaccggt ctggactcca gcaacatcta
     1741 caaatctgac ccagatattt ttcataatca agtcaccata aactgcgatc ataaaagtct
     1801 tgtttacgct ggtactcgcc gtccagagca tctgaggcgg tgatgtttcc tgagttgctc
     1861 caatatcctc gccgacaagt tccagaacct gtccgaccgg cacttcaata acaccagtca
     1921 taacgtacca gtcattcgat tttaatttaa aaacagtatt ggataatgtt ccttcagtat
     1981 taagaacagt tttaacggca tcgttcagag aaccttctaa accaccacca taaataccgt
     2041 ttacttctac aacagccttc tcttgtgcat ttagtcctac agtcagaaaa attgcacaaa
     2101 gtaaaaatac taacattctt ttcatgtttt tgacctcctg aattttttgt ttcgagtatt
     2161 ttggcattat tttcatttta aaaactttct ttctcctttg acttataatc taaatctgat
     2221 tcctaggtta gccgtcatac cataatgctt tacattggta aagccatcaa tagacatctg
     2281 agccgatgaa ttaacttcat tattgagatt attgacatcc aaataaattt ccgaaccaag
     2341 ccatggtaga gtctgtcgaa cggaagcatc tatccggaaa taatctctgg tgtatccatc
     2401 ttgctccgga aaagtaccta cgtaactaac ggaattaccc tggaacaaga atgataaccg
     2461 ggctgaaaag cctttatatt cataaccgat gtaggcgtta accacgtcat tcggctgata
     2521 gagcaaacgt ccggtacgag tggaatcacg agtcgtagtt atcggcggcc taacggtata
     2581 atcagtcact tcctctctca aaggataagt ggcttctgat tcgatcatcg tatagttaac
     2641 acccagaact atcccatcca aaccccatgg tagataccag aatctggttt gccagtcaaa
     2701 ttcgagacct tttacataag ccagatagcg actattaata taggtatata aagtagctcc
     2761 ttcctccggt ttaatcccca ttatctgaat atcattaatc gtcttaatgt cgcttgattt
     2821 ggaagtttta tggaggatat aagccgtact ataaacaaaa tcctttatgg ttttataaaa
     2881 accaccgatt gaaagaaggc ctaacttatt gccgtgaaag gtcaagttca aatcgtgatt
     2941 ataagcatgc gccggtacta aatcgggatt accggctcgc acacttcttc tggtattatc
     3001 atagtttatt ttaggcgaca tctggtgata atccggtcga gcaagggttt gagtgtaggc
     3061 ataacgaata tcgaaccact cgaaaggtga gtatttagct tgaaccattg gcaaccagaa
     3121 ttcgcttttt ggataagaat agactgtatc gcaattctga gcatcagggt tgcgcatatc
     3181 gtacatattg taggcggtat atttagactc ggttttttcg taccgggctc cgccaactat
     3241 cattaaattc ctgaagtcaa tctccgacat tacataaccg gcataatatt cttccctata
     3301 tctatatgaa ttagctaaat tctggaacat tccattatac caacctccgg tctgatcggc
     3361 tccaccggta gcttttcctc tccattgggg attattattc aaatagtctg ccatctccac
     3421 cagaatatcc ggcttacaag cccagtagat ttctccaaat tgattgtcca gaaaaggttg
     3481 catcaatttg gaactgccgg taaaattgga agccgcaaat ttggcggtac tgggatcaac
     3541 aggaatacca aactgtctct ccaaatcttc gaccatcgcc gcctgaaact caccactttt
     3601 cctgatttca gcataaggtg ttttctgatc attagtattc ttctgattat aatactgacc
     3661 gccgaattta aaataaccta aaagagactt cccaatacta aaaggaactt tgaaatcgga
     3721 agagaaatta tcattattct ccgtatataa agaactgaac aaattcaagt aatctaaata
     3781 aactttgtca tggccaggat aaacccataa tctggtcaaa ctatccggaa cagtattaat
     3841 cgggacgcta cccatcatcg cctggccggt tttgaaagtt atatagggag attctggcaa
     3901 atcattcctt gaatgagtat tggccgcttt taaatccatt tgaatatatc gaaaatcata
     3961 ttttaattcc agcgaattta ccgacaaatc agtcgtattt attccacctc tataattaaa
     4021 attgagaatt ttatcgaaat aatttaaaac ggtattataa tcctgataat cggagacaaa
     4081 gcgggtatac atattcacgg acttaatcga accggcaggc aatttataat ctaaaattaa
     4141 attaccacca taacgcttgc gggtttcaac atgtcggtta agggttacac tattaacact
     4201 aaccggtcgg taaccagtag aagtatcaat gacactacta gtaactcgat agttggcgct
     4261 catattgtca gcatctcgat catatttctc ggcgttaatg aggagatatg ctcccagatt
     4321 atcctggaaa aagcgctggc taatggagcc gaccgcccgg taattgccgt aattgtttgt
     4381 tttggcggtg tagccggatt gccacatcaa atcataatgc agttgtgaag gcgcttccct
     4441 caattccata tttaccgacc caccaatgga gttggcattc atatccggag taagcgactt
     4501 aaaaaccgaa atggatttaa tcatataagg cgagatcatc gagagatcaa cactgcggtc
     4561 attcgaaata cttccagcag tattgcccag agcggaaact cctatctgag tgctaccggt
     4621 tgaagccagt tttatgcctt caactgccac ggcgttatat tgcggtgcca agccccggat
     4681 gaccacttta ttggcttcgc ctgaactctc cagagtcgag acgcccggca agcgactaag
     4741 agcttgagcg gcattaaagt ccggcaattc ctgaatacga gcttcggata caacgctggc
     4801 aattttatca gaggctaatt gctggttaat ggcttgcatt tgccccaaag cctgagcggt
     4861 tacaataact tctttacctt gtagaacttc cggttccaaa ccgaaatctc tccgcaccgt
     4921 gccaccgtct ggaatattta catcaacttc gagtgttaca taaccaatat aagaaatcac
     4981 aatcgtctgc ggcccatccg gagcattcgt aatagaatac ataccatcgg catccgccgc
     5041 cgttcctatg gttgttcctt taatcattat gttggcatac gatagcggat ctttggtatt
     5101 tttgtcaaac actctccctc ggattgtacc cttagcccaa agagactggg tcattaggca
     5161 cagaactaag ccggcgataa aaaatgcttt acacccacga cctcgggtca tcttaataaa
     5221 acgcacagaa atcttcataa gactctatcc tttttactgt ttcgtaaatt tttttcatag
     5281 tcccacatat ctattagatg tcttatcaat taccttctca attttaaaga tataatttat
     5341 gaaaggcaac tgcgttcttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     5461 tttttttctt gaaagcaagc gagataaata ttattttagg tttagaggta attaatatgg
     5521 atcgaagaaa atttatcaaa actagcgcct tcctaaccgg aggaatggtt ctgagaggtg
     5581 ctcagattct cggtaaagaa acaacaattc ccgatttagt aatggttaag gacggtcaac
     5641 cggctgaaat ggcacagaaa accttcgaac ttctcggcgg tgttccccgc ttcgttgcca
     5701 aaggcgacta tgttctatta aaacctaacc tgagctggga tcgcgctccc gaatatgccg
     5761 ctacaactaa tcctgagctg gtagctgaaa tcgtccgtct atgtttccaa gctggtgcca
     5821 aaaaagtcgt aattctcgat aatacctgtg ccgatgcacg acgtagttac gatgcctgcc
     5881 aaatcccggt atatgctcgg caggcgggcg ccgacgtacg ctttgtgcgc gagaatcaat
     5941 ttgtcgaaac accgattccg aacggaacca atttaaaaag ctggccaatt catcaagaag
     6001 tttttaatgc cgataaactg atcagcatcc ccattctgaa acaccatgga atccccggaa
     6061 ttacctccgg ttttaagaat atgatgggct tactcggcgg tcagcggggg cgaattcacc
     6121 gaccattttc ggaaaatatc gttgatatta accgggtaat caaacctaac ttaaccatca
     6181 ttgatgctat ccgtgttcta cgacggaatg gaccacaagg cggtaatctg aacgatgttg
     6241 aacaaatgaa cattttgctg gctggaacgg atcgagtctt ggtggatgct tgggcggcga
     6301 aaatattcgg cctcaatcca cttaccgtag aatatctgaa gctggcacat tcagccggtt
     6361 tgggtgagat tgatactgaa aaattcaaac cggtcgtcta ttctttcaaa gcatgaaaac
     6421 caaatcgctt acgtggcgca aaatatcgca ggtctttttc ttcctgctat ttacgctaac
     6481 cctccttcag gcaacttatc ctttcaaacc ctggttgccg ccggagctgt ttctctggtt
     6541 tgacccattg gcggcaatta ccactcaaat agccggtcgc ttcttcgata ttgtgtttct
     6601 attagcggca attatcttaa tcagtccatt gattttcgga cgtgctttct gcggctggat
     6661 ttgcccgatg ggaactctta tagatgtcag tgatcatatc ctgaagccga aaaaaaggcg
     6721 cgattttcgc catctgcgct ttttgaaatt ggcaattctc accgccgtga ttgtattggc
     6781 gattttcggt gtgcaatatg cctggctatt agacccgttg ccggtcgcct ggcgctctat
     6841 tggtgtaatc ggtctggctt ttttcttcct gttaagtgac tcgatcttaa gtggtttaat
     6901 cagtattgga atttttccgg atgctctgct aagttttcag gacaaaatag caggatttct
     6961 cttcccggta gcgagtccaa atttcggtgg attggttgcc ccgttgatta tttttcttat
     7021 aatactgggc ttaagccgtt tgagtcggcg cttttggtgc cgctacctct gtcctttagg
     7081 agctttgctg ggtctggtgg cgcgaatcag ccctctccgt cggattgttg atgccgacaa
     7141 atgtagcgct tgtggcattt gccgacataa gtgtaaaatg gacgccattg aacccgattt
     7201 tgtgacgact actaaatccg aatgtattct ctgcctgaat tgcaaatatg actgtgcgac
     7261 cgaggcgatc agcttcggct ggcgggcacc tacccagatg atgaataaag ttgacctctc
     7321 gcggcgcgca ttcatcggta caagtttgac tgctctggct ggcgtcggct ttttaaaaat
     7381 taataaagtt aacccgcgtc attctgaccg cttaattcgt cccccgggcg ctttgaacga
     7441 ggatgatttt ctggaacgct gtatccgttg cggcgaatgc gttcgcattt gctctacttc
     7501 tggaaaatgt ttgcaatttt ccgtattaga aagtggattg gaaggtttta tgacaccatt
     7561 agccgaatac cggcgcggct attgcgaata caattgcaat ctctgcggac aaatttgtcc
     7621 aaccaaagcc atccagccat taaacgtttc ggcaaagcaa aaagccaaaa tgggacaggc
     7681 aattttcctg aaagaccgtt gcattcctta ccgattgaat gaaaattgca tcgtttgcga
     7741 ggaacattgt ccgattcccg acaaagccat caaaatcaat acacgcgaat acgaagatcc
     7801 ggtcaccggg cggaagcgta tggtcagtta tccgtatgta gatgaagctc tctgtatcgg
     7861 ttgcggcatt tgcgaaaata aatgcccact caatggcgaa gctggcataa tcgtcattcg
     7921 cgaaggcgaa gagcgatcgc gagcttaaat aactcaaata attcgagcaa aaatatccac
     7981 ctccgttggg aaattctccg gaaatagaat ccgtcgagcg ctggattcgc gcgaatcaaa
     8041 atgatttgaa aaaaatttat accaaccgca ttatcaatca ttaccaatct gaacaaaaat
     8101 gaaaataccc attgtcccaa ttttgtttta tattatcata gaaataaatt tggataggtc
     8161 aacgcgatgc gtaatttgaa caataaaaat cgttatttaa cagcgttggc attcgattgg
     8221 taacaaactg atgtagcgtt atggcaatca cacgccgtca attttgcttg aaacttggaa
     8281 gtggggtagc cgcttccgtt ttcgttcccc gtctaatcaa aagcggctgg actaaaccag
     8341 cgactgattc caaaccttac ctccgtgaag ctcgctatta cactcctctt gccgaccgaa
     8401 aagtccgctg tttactctgc ccgcgcacct gtttggttgc cgatggtaaa cgcggttatt
     8461 gccgggttcg tgaaaatcaa aaaggcagct ataagactct ggtttacggg cgactttgct
     8521 cgattaacaa cgatccgatc gaaaagaagc cgcttttcca ttttctgcca ggcactatgg
     8581 ctctctccgt cgccaccgcc ggttgtaatt tgaaatgtaa attctgccag aactggaata
     8641 tttcgcaagt agcacctgaa gacattccgt ttgaatatta ttcacccgag agactggtca
     8701 atctggctca gcaaatccaa tctcctacca tcgctttcac ttacactgaa ccgacgattt
     8761 caaccgaata tatcttggat actgctaaat tagcgcggtc taaaggcatt cattccgtta
     8821 tgatttctaa tggctacatc aactccgaac cgcttggcga tttatgccaa gtgctctcag
     8881 ctatcaaaat tgatttcaaa gcattttccg acgactttta tcgcaatgtt actgacggta
     8941 ctcttcaacc ggtgctggat acgatgattc agattaaaaa gaacaatgtc tggttagaga
     9001 ttgtcaatct ggtaattcca acccagaacg atgcgcctgc tcagtttcgg cagatgagtc
     9061 agtggatttt gcaaaacctt ggttcggaca taccagtgca cttcactcgc tttcatccct
     9121 tatatttaat ggaaaatcta ccgccgacgc ccttacgcac actggaatcc gcttatgaga
     9181 ttgcccgcga atgcggcttg cgattcgttt acgtcggcaa tgttccggga catgccgcgg
     9241 aaaataccta ttgcccgcat tgcaatcgca aaatcatcga acgaatcggt tatcagattc
     9301 gcacttttgc gattaagaat ggcaaatgcc aatattgtca aacaaatatc cccggatttt
     9361 ggagttgaca atgaaaaaag gagtaattat tatgacacta attttatcaa tgtttacaaa
     9421 catcacgtct gctcaggaac gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 ngtagataag aaactcgccg cccagtgcgt aactgccgat aggctggtta aattagcgac
     9601 tgaaggacat ttaagtcaaa gattcggacg cgccgaacac agtctggaag tccaactgcc
     9661 ttttctgcaa atcgttttac ccaatactcc gatcgtaccg attgttattg gaactcacga
     9721 ctggcgagta ctcgaatcac tcggcaatcg gctaggcgaa ctgcttgtgc agaaaaacat
     9781 cctgattgtg gcatccagcg atttttcgca ttatcattcg tacagcgagt gccagaaaat
     9841 tgatcagcgc gcgattgaat ccctcaagca gttagacgcc gtgaaattct accaaggctt
     9901 tacgtctgaa acattcgaag cttgcggggg cgcgccgatt accgctttgc taattgccgc
     9961 tcagaaaatg aaaaatccgc agatcgaaat cctaaaatct gccaatagcg gcgatgttcc
    10021 ccacggcgac aaatcggcgg tcgtcggcta tgttgcggtc ggactttatc aaggcaccaa
    10081 agctaagagc caagatgaca atacagcgag cagtcaattg aatacaggcg atttgacgcg
    10141 ggaggaacag ctttatttgc tcgatctggc tgagaaaacc gtcgccgccg ctgttaatca
    10201 cagacctctg ccggatccca aagacgttcc ggaacgcctg aaagtcaaac gcggcgcttt
    10261 cgttactctc gaaaagcatc acgaactgcg aggttgcatc ggttatattc tgccgattta
    10321 cccgctctat aaaaccgtaa tcgaaatggc taaagcggcc gccctggatg atccgcgctt
    10381 tccacccgtc tcaccgagtg aattaaaaga catcactgtg gagatttccg tcctgaccgt
    10441 tccgcaaaaa attagcgacc cctcggaaat cgaagtcggc aaacacggca tcattattaa
    10501 acgtggcttc aatcaaggct tgcttctacc gcaggtagct acggaatacg gctgggatcg
    10561 cgagaccttt ctcgaacata cctgtctgaa agccggctta ccgcgcgacg cctggcagga
    10621 taaaaatacc gaaattcaaa ttttctcggc tcaggtattc aaccgtgaga cggtgaagta
    10681 acaaacaatt aattag
//
LOCUS       DAOOMU010000078        10297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_7422_length_10297_cov_19.309900, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000078 DAOOMU010000000
VERSION     DAOOMU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7422_length_10297_cov_19.309900"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..957
                     /locus_tag="PLI55_07805"
     CDS             52..957
                     /locus_tag="PLI55_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB00779.1"
                     /translation="MKKSILVVGSVAFDDIETPVGKRFNALGGSAVYFSLAASQFSQV
                     NLVGVAGEDYPKAAIDLLNRQGIDTTGLEIAPGPTFRWGGRYHQDLNNRDTLYTHLNV
                     FANFSPKIPSVYQKTPVVFLGNIQPTLQMEVLNQLPQPEFIGLDTMNLWINTTRAELM
                     KIISRVNLLVINDSELEELTGTVNLFKGLKYLHKIGVRHIVIKQGEYGAMLSAAEGLF
                     FTPAYPVEEVVDPTGAGDSFAGGLFGYLGTCEKIDYDNLKKAVVYGSVMGSFCVENFS
                     IDGLLNLNPTKIAKRFRELRRLVKA"
     gene            979..1998
                     /gene="mtnA"
                     /locus_tag="PLI55_07810"
     CDS             979..1998
                     /gene="mtnA"
                     /locus_tag="PLI55_07810"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPB00780.1"
                     /translation="MIQPLFWENERLLIVDQTRLPFEYKLIEIHDDHEMADAIRRMVI
                     RGAPAIGIAAAYGLALGLKKFILGNHEEFFRQLEIISNRLYETRPTAVNLGWALNRLK
                     RLAELNRHRKLIEIQTILLNEAHTIHTEDIQTCRQIASRGLSLLPDDVRVITHCNAGG
                     LATGGLGTALGIIISAHQSGKKIHVYVDETRPRLQGARLTAWELTQEKVPFTLISDNM
                     AAFVMARNRIDAVIVGADRIAANGDTANKIGTALLAIAAQYYRIPFYVAAPTSSIDPL
                     TPSGAAIQIEERDGTEVKEINGQPIAPVGCSAISPAFDVTPARLITAIITEKGIYQPP
                     YRLIE"
     gene            2075..3124
                     /locus_tag="PLI55_07815"
     CDS             2075..3124
                     /locus_tag="PLI55_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00781.1"
                     /translation="MKPRKISFVFVLLLACLFVLDACDPPEITSAKVYLQQNNLQAAE
                     EQLLQAIQKYPDNAQAYLLLGSAIYRPTKRYDEAKQMFEKAKQIDPTKAKEADDNIRG
                     MWAELHTEGANLFNSALKAFLPSEKDSLLNLAAQQFIRALEFKSDEIPTYNGLVKCFY
                     ILNDSANVEKYGKMMLDKNLFDEDVVNYYFQVAWDSTRQEEIFAQLDKIIAEHPDNIE
                     LQILKIQFLSDVGRNEEALDIINKLIENDPTNLDLVYIAAQLLTKLGRFDEAKYQFQK
                     VIANDPTNLELLIRVTEAVFRDKDWVEAEYYARKIIELDPNNVFGYEVLWKSLYNQGN
                     MEEAEKYRMIQKSLE"
     gene            complement(3176..4081)
                     /locus_tag="PLI55_07820"
     CDS             complement(3176..4081)
                     /locus_tag="PLI55_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPB00782.1"
                     /translation="MAEERLDKRRYLEPAVIARLDNMALRARLVVEGFIIGLHRSPYH
                     GFSIEFAEHRPYMPGDEIKHIDWKLYGKTDRFYIKEFEEETNLKSYIILDKSGSMGYQ
                     STAVSKLAYGSYLAAALGYLLLKQQDAVSLTLFDTEITTYLPPVAKKSHLNLVLGALE
                     KVTAGSETSIAPLLHSAAEKIKKRGLIILISDLLDDPSSVINALKHFRHRRHEVIVFQ
                     VLDPQEIEFGFNRQTKFVDLENRDALLTEPGQVRLAYRQAINNFIEDYKIQCRANNID
                     HVLLTTDRPLDLALTEYLNKRARIG"
     gene            complement(4102..5091)
                     /locus_tag="PLI55_07825"
     CDS             complement(4102..5091)
                     /locus_tag="PLI55_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPB00783.1"
                     /translation="MEQLNDVELLTALTKANERLNKEIGRVIIGQQEIIDQIVISLLS
                     RGHCLLIGVPGLAKTLMIKTLAQALDLQFRRIQFTPDLMPSDITGTEVIEEDKLTGAR
                     AFRFFKGPIFGNIILADEINRTPPKTQSALLEAMQEHRVTAAGETHQLEEPFFVLATQ
                     NPIEQEGTYPLPEAQLDRFMFSLKVEYPTFQEELEIVKSTTVDADSTPEIVIHRAEIL
                     QFQHLVRRVPVAPNVIEYAVKLVTATRPTNSSAPDFIHKWVEWGAGPRASQYLILGAK
                     ANAVLEGRPTPSIQDVIKMAKPVLRHRIIPNFNAEAEGINTDHIIQKLLETIG"
     gene            5456..6964
                     /locus_tag="PLI55_07830"
     CDS             5456..6964
                     /locus_tag="PLI55_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPB00784.1"
                     /translation="MPASYLTEPRDFHSISLWQQITEKQWHDPIWQRKNAIRDVDELC
                     QVIRLNDYQEGEIAHTIAKLRAEGKEPMRITPYYASLIAEDPFHPNLLPSEEAENRLD
                     PIFWEAVPTPAHLLFPHTGSEGAMAEENRSYGSAYQRYPNRVALFVAENTSCASYCTH
                     CQRLKSLDCTTTISRQDIDKGLFYIDWNPNIDEVLVTGGDALMISPERLEYVLTSLSK
                     IEHLRVIRIATRVPVTLPMAITDDILSLIDKTTSNSGKYVYFMTHINHYHEITPEFKS
                     AIDRIHRHGYSIRNQTVLLKHVNDNLGSLAETLRRMFWAGVEPYYLLQCHRERGLVHF
                     ITPIQIGKIFVKHLQGWISGMARPVYAANIEGGGGKIILMPSGHDTMQKGFNLEEYIS
                     ESYATIHTWDNKIIDDYEALGRATQAEYDAAVKIMDKFIGRKGAFRPSLIIVDKNGNH
                     ITTTNKSPLPSFSNRDKVKIFNYWVDSDDMPLTNPALISDKLDKLYTNSKLE"
     gene            7142..>7614
                     /locus_tag="PLI55_07835"
     CDS             7142..>7614
                     /locus_tag="PLI55_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00785.1"
                     /translation="MLKIYRRRQFNSTFLVVFSLGLIVLLLNDCTTRPQTSVAQYQFT
                     FEGEVYRIRSIHAVENNERYNELIGKNFLAVDFDQDRIIDKINVGTTTLAEAQKVYDY
                     GLEMLTKENRLKEITPENHKYIEDLEDFHLELKSFYPVNAKPFNQFKIISKKQLV"
     assembly_gap    7615..7714
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7715..8162
                     /locus_tag="PLI55_07840"
     CDS             <7715..8162
                     /locus_tag="PLI55_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPB00786.1"
                     /translation="WSSICFSQEKTANIQIFTSEFERIEIVRLRSGMDLLEGLKEAVS
                     SRKIKNGVILAGIGAVTDYHYHVVSSKNLPPDEEYPKASVPMDLISVQGYILNGRVHA
                     HIALSDANSVVGGHLESGTKALTFFIITIGVLPDSLKIDNLDNYKY"
     gene            complement(8249..9826)
                     /locus_tag="PLI55_07845"
     CDS             complement(8249..9826)
                     /locus_tag="PLI55_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HPB00787.1"
                     /translation="MKKLVIGILGIFLMQNLYAQNVENKIVIDVEAGQHMISRHIYGH
                     FAEHLGRCIYGGIWVGEDSPIPNTRGIRNDVVAALKDIAAPNVRWPGGCFADEYHWMD
                     GIGPRDQRPKMINTHWGGVTEDNSFGTHEFMDFCEQVGCEPVITGNVGSGTVREMAQW
                     VEYLTSDNISPMTELRKKNGREKPWRVRFWGLGNENWGCGGNMTADFYADEMRRFSTY
                     CKNYSGNELYRVACGPSDENYEWMETLMKEPINRNCFQGISLHYYTVCHDWTNKGSAT
                     NFNEKDWFLTMSKTLRMDEFVTKHSAIMDKYDPEKKIGLIVDEWGNWHDVEPGTNPAF
                     LYQQNTLRDALVAATNLNIFNNHCERVKMANIAQMVNVLQAIILTNEDQIVLTPTYYV
                     YKMYKVHQDATLLPIELTCQNYELDGGSIPAVNASASVDKQGVIHITLCNLDPNKNMN
                     LTCELKSKNKFSVSSGSIITAGKMNAFNDFGKPEEVNLQKFTDCKIAQNTLSVNLPAK
                     SVVMVELVSKSADKPKK"
     gene            complement(9857..>10297)
                     /locus_tag="PLI55_07850"
     CDS             complement(9857..>10297)
                     /locus_tag="PLI55_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative collagen-binding domain-containing
                     protein"
                     /protein_id="HPB00788.1"
                     /translation="VGKRHHIWEALKFESANYMKHLSSFILSEGNKYQDLRLAREDIH
                     PNKAPNSPPTGLDGWAYMMRTPDKNLAMLYFENQSVLPTLSGFKPNTMYHFKWFDPRN
                     GEWKEAQTIQADKKGNLKVPLFPDGNNPSIKDWAAKICKEEKNK"
BASE COUNT         2924 a         2212 c         2119 g         2942 t
ORIGIN      
        1 aaagtatttt tagcttcggt ggttaattga taataaaata aggaaatagt tttgaagaaa
       61 tctattctgg tagttggttc cgttgctttc gatgatatcg aaaccccggt tggcaagcgt
      121 ttcaatgccc tgggtggttc ggccgtttat ttcagtttag cggcttctca attctcacaa
      181 gtaaacttag tcggcgtcgc cggtgaagat taccccaaag ccgccattga cctgctgaac
      241 cgtcagggaa ttgatactac cggtttagaa attgctccag gaccgacttt tcgctggggc
      301 gggcgttatc accaagacct caataaccgc gacactcttt atactcatct taatgtcttc
      361 gctaatttca gtccgaaaat cccctccgtt tatcaaaaga caccggtcgt cttccttggc
      421 aacattcagc cgactttaca aatggaggtg cttaatcaac taccccagcc ggaattcatc
      481 ggactggata caatgaattt gtggattaat accacgcgcg ccgaactaat gaaaatcata
      541 tcgcgagtta accttctggt aatcaacgat agtgagctgg aggaattgac tggtacagtt
      601 aacctgttca aaggtttaaa atacttgcat aagatcggag tacggcatat cgttatcaag
      661 cagggagaat acggtgcaat gctcagcgcg gctgaaggct tatttttcac tccggcttat
      721 ccagttgaag aggttgtaga tcctaccgga gccggtgata gttttgcggg tggactgttt
      781 ggttatctcg gcacctgcga aaaaattgat tacgataatc taaaaaaagc ggtggtttac
      841 ggcagtgtta tgggttcatt ctgtgttgaa aatttcagta tagacggttt attgaatctt
      901 aatcctacca aaatcgcaaa gcgatttcgc gaattgcgcc ggttagtgaa agcctgagct
      961 caatcggact aattgcttat gatccaaccc ttattttggg aaaatgaacg cctgctgatt
     1021 gtagatcaga cgcgcctgcc gtttgaatat aaactgatcg agattcatga cgaccacgaa
     1081 atggcggacg cgattcgccg tatggttatt cgtggcgctc cagctatcgg cattgccgcc
     1141 gcctatggtt tagcgctggg gcttaaaaag tttatcttag gtaatcatga agaatttttc
     1201 cgccaattag aaataatttc caaccgactt tatgaaaccc gtccaaccgc cgtcaatctt
     1261 ggttgggcat taaatcgtct gaaacgactg gctgaactca atcgccaccg gaaattaatt
     1321 gaaattcaaa ccattttact taacgaagcc cataccattc atactgaaga tattcagacg
     1381 tgccgtcaaa ttgcttctcg tggtctgtcg cttctacctg atgatgttcg agttatcacg
     1441 cattgcaatg ccggcgggct ggcaaccggc ggactgggaa ccgctttagg gataattatt
     1501 agtgcccacc agagtggcaa aaaaattcac gtttatgtgg atgaaacccg cccaaggttg
     1561 cagggcgctc gtctgacggc atgggaactc acccaggaaa aagtgccctt taccttgatt
     1621 agcgataata tggccgcctt cgttatggca agaaaccgaa ttgatgcggt tatcgtcggc
     1681 gccgatcgga ttgcggctaa tggtgatacg gctaacaaaa tcggaacggc tctactggct
     1741 atcgcggctc aatactaccg gattccgttt tatgtagccg cgcctacttc aagcattgat
     1801 ccactcactc catccggcgc ggcgattcag attgaagaac gtgatggtac tgaagtcaag
     1861 gaaataaatg gccaaccaat cgccccagtc ggttgctcgg caatttcacc ggctttcgat
     1921 gttactccgg cacggctaat tacagcaata attactgaaa aaggcattta ccagccgccc
     1981 taccgtttaa ttgagtagtt gaattttgtg agcatttttg taaattttaa tgtgatttgg
     2041 aaaaaatatt cagattgata aaggagtaag aataatgaaa ccgagaaaaa tttcttttgt
     2101 ttttgtctta ttactggctt gtttgttcgt tctggatgct tgcgatccgc cggaaattac
     2161 ttcggctaaa gtttatctcc agcagaataa tctgcaagcc gccgaggaac aattactgca
     2221 agctatccag aaatatccgg ataatgccca agcctatctc ttattgggta gcgcaattta
     2281 tcgcccgact aaacgctatg atgaagccaa acagatgttc gaaaaagcca agcaaatcga
     2341 tcctaccaag gcaaaagagg ctgatgacaa tattagagga atgtgggctg agttacacac
     2401 cgaaggcgcc aacttgttca acagcgcttt aaaagctttt ctaccgtctg aaaaagactc
     2461 gctcttgaat cttgccgctc agcagttcat tcgtgcattg gaattcaagt ccgatgaaat
     2521 tccaacttat aatggcttgg taaaatgttt ttatattctg aatgactctg ctaatgtcga
     2581 gaaatatggc aaaatgatgc tggataaaaa tcttttcgat gaggatgtcg tcaattatta
     2641 ttttcaagtt gcctgggata gcacccgtca ggaggaaatt ttcgctcagt tagataaaat
     2701 tatcgctgaa catccggaca atattgaatt gcaaattctt aaaatccagt tcctatccga
     2761 cgtgggacgc aatgaagaag cccttgatat aattaataaa ttaatcgaaa atgatcccac
     2821 caatctcgat ctggtttaca tcgccgctca attactgact aagctcggcc gattcgatga
     2881 agctaaatat caatttcaaa aggttatcgc taacgatccc actaacctgg aattactaat
     2941 ccgcgtaact gaagcggttt ttagagataa ggactgggtg gaagcggaat attacgctcg
     3001 taaaattatc gagctggacc caaataatgt attcgggtat gaagttctct ggaaatcgtt
     3061 atataatcaa ggtaatatgg aagaggctga gaagtatcgt atgatacaaa aatcgctgga
     3121 ataagcctgc tgattaattt ttttaaaagc cggactaaag atccggcttt tttttctaac
     3181 caattcgggc gcgtttattt aaatattcgg tcaaggctaa atccagaggg cggtcggtag
     3241 ttaataagac atgatcaata ttattcgccc ggcattggat tttataatcc tcaataaaat
     3301 tattgatcgc ctgccgatat gctaatctca cctgaccggg ttcagtcaat aacgcgtcac
     3361 gattttccag atcaacgaat ttagtctggc gattgaaacc aaactcgatt tcttgagggt
     3421 caagaacttg aaacacgatt acctcgtgac gtctatgacg aaaatgcttc agggcattga
     3481 ttacgcttga agggtcatcc aacaaatctg aaataagaat aatcagcccg cgtttcttaa
     3541 tcttttcagc ggcggaatgc aggagtgggg ctatggaagt ttccgaaccg gcggtgacct
     3601 tctccaaagc tccgagtact aaattcaagt gactcttttt agcgacgggt ggtaagtagg
     3661 tagtaatttc ggtatcaaaa agagtcaggc taactgcatc ttgttgttta agtaataaat
     3721 atcccagcgc ggcggctaaa tagctgccat aagccagctt ggaaacggcg gttgattggt
     3781 agcccatcga accgctttta tcgagaataa tataggattt aagattggtt tcttcttcga
     3841 attcttttat ataaaagcga tcggttttac cgtaaagctt ccagtctata tgtttgattt
     3901 catcgcccgg catataaggc cggtgttcag caaattcgat cgaaaagcca tgataaggac
     3961 tccgatggag gccgattata aagccttcca ctaccagtct ggcgcgcaaa gccatattat
     4021 ccaaccgagc aatgactgcc ggttcaagat aacgccgttt atccagtctt tcttctgcca
     4081 tttataaaaa cattccccag attacccgat agtctcgagt aacttttgaa taatgtgatc
     4141 ggtattaatt ccctcggctt cggcattaaa gttcggaatt atacgatgcc tcagcaccgg
     4201 cttcgccatc ttaattacgt cttgaattga cggagttgga cgaccttcca gaacggcatt
     4261 cgctttagcg ccgagaatca aatactggct ggcacgcggt cctgctcccc attctaccca
     4321 tttgtgaata aaatcaggcg cgctggaatt agtaggccgc gtagcagtta ccagcttcac
     4381 cgcatattca ataacgttcg gagccaccgg cactcgtcta acaagatgct gaaattgcag
     4441 aatttcggct cgatgaatga caatttccgg tgtggagtcg gcatcaactg tggttgactt
     4501 gacaatctcc agttcttctt gaaaggtcgg gtattcaacc tttaaactaa acataaaacg
     4561 atcaagctgc gcttctggca acgggtaggt gccctcttgc tcaatcggat tctgagtcgc
     4621 taatacaaaa aacggctcct ccaattggtg cgtttcaccg gcggcggtaa cgcgatgttc
     4681 ctgcatcgct tccagcaaag cagattgggt tttaggtgga gtgcggttga tttcgtcagc
     4741 gagaataata ttgccaaaaa tcggcccttt gaaaaatcga aaagcgcgag cgccggtcag
     4801 cttatcttct tcgataactt cggtgccggt aatgtcggac ggcattaaat caggtgtaaa
     4861 ttgaatacgc ctgaattgca ggtcaagcgc ctgcgctaag gttttaatca tcagcgtctt
     4921 agctaatccc ggcacgccaa tcaaaagaca atgtccccgt gaaaggagag aaatcacaat
     4981 ttggtcaata atttcctgtt ggccaattat cactcgccca atttctttgt taagccgttc
     5041 attggctttg gttaaagccg tcagcaattc gacatcatta agctgttcca tatatttcca
     5101 ttccgattat aaaattgaaa gcgttgagaa tctaagactt tccgctcaat ttgccaataa
     5161 ttgccggtgc ctgttagatg ataaataacc aatttgataa attctccctg agcgtcagcc
     5221 cggtacatta gaattttaac ccaatataaa tttatataaa cgtagtgggg tctggcataa
     5281 taatttctaa tcagcttctc attttaatcc caaaaatttc ctgctgcact ggcaaggctc
     5341 tcgattttat ataagcaagt aggatttgca taaaaaacga ctgtttttta gattccaaaa
     5401 gaataatgac attttataat aatttaagct acataaaact taggagaaat taacgatgcc
     5461 cgcatcttat ttaaccgagc cacgcgattt tcattcaatt tcactctggc aacagattac
     5521 tgaaaagcaa tggcatgatc ccatctggca gaggaaaaat gccatccgtg acgttgacga
     5581 actatgtcag gtgattcggc taaacgatta tcaagaaggt gaaattgccc atactatcgc
     5641 caaattacgt gcggaaggca aagaaccgat gcgcattaca ccttattatg cgtccctaat
     5701 tgccgaagat cctttccacc ccaatctatt acctagcgaa gaagccgaaa atcggctgga
     5761 tcctattttc tgggaagctg ttcctactcc ggctcatcta ttattccccc acaccggaag
     5821 tgaaggtgca atggctgaag aaaatcgcag ttatgggtct gcctatcaac gttatcctaa
     5881 tagagttgcg ctcttcgttg ccgaaaatac cagctgcgct tcttactgta cccattgtca
     5941 gagactaaaa tcactcgact gtactactac tatcagtcgg caagatattg acaagggact
     6001 attttatatt gattggaatc ctaatattga tgaagtttta gtgaccggtg gcgatgcttt
     6061 gatgattagt cccgaacgcc tcgaatacgt cttaaccagt ttatcaaaaa ttgaacattt
     6121 gcgggttata aggatcgcta ctcgcgttcc agtaacgttg ccgatggcta ttactgatga
     6181 tatattatct cttatagata aaacaacttc taactccgga aaatacgttt acttcatgac
     6241 tcacattaat cattatcatg aaattactcc ggaattcaaa tccgcgattg accgcattca
     6301 ccggcatggc tattcgattc gcaatcaaac cgttttgttg aaacatgtca atgataacct
     6361 cggttcactc gccgaaacct tgcgacgaat gttctgggct ggagtagaac cttactatct
     6421 acttcaatgt catcgagaac gaggtttggt gcacttcatt acgccaattc aaattggcaa
     6481 aatttttgtg aaacatcttc agggttggat aagcggaatg gctcggccag tttacgccgc
     6541 caatatcgag ggtggaggag gaaaaataat tctgatgcct tctggacacg ataccatgca
     6601 aaaaggtttt aacctcgagg agtatatctc agaaagctat gcaacgatcc atacctggga
     6661 taataagatt attgacgatt acgaagcact cggacgcgct actcaggctg aatatgatgc
     6721 ggcggtcaaa ataatggata aatttatcgg gcggaaagga gctttccgac catctttgat
     6781 tattgttgat aaaaatggca accacattac gacaactaat aagagtcctt taccaagttt
     6841 cagcaatcgc gataaggtta aaatttttaa ttattgggta gattctgacg atatgccttt
     6901 gactaatccg gctttgatta gcgataaact cgataagtta tataccaact caaagttgga
     6961 gtaattaaaa tcagtcgggc tatttgtagc actctaacta attcaccggc taaaattaca
     7021 ggtagctttc tccgtaaaat atttatattt ctttgaaaaa catttgcttt aaatttgaat
     7081 gtgatttagt ctttgataat aaaataaatg aagaacaaat cttttaaaag gagaaaaagt
     7141 catgttaaag atttatcgcc gtaggcaatt taattcgact tttctggttg tttttagtct
     7201 cggtttaatt gtactgcttc ttaatgattg cactactcgc ccgcaaacca gcgtggctca
     7261 gtatcaattc acctttgagg gcgaagtcta tcgcattcgc tcgattcatg cggtcgaaaa
     7321 taatgaacgc tacaacgaat taattggtaa gaatttttta gctgttgact ttgatcagga
     7381 tcggattata gataaaatca acgtcgggac caccacttta gcggaggcac aaaaagttta
     7441 cgattatggt cttgaaatgc tgaccaagga aaatcgcctt aaggaaataa ccccagaaaa
     7501 tcataaatat attgaagacc tagaagactt tcacctcgaa ttaaaaagtt tttatccagt
     7561 caatgctaaa ccgttcaacc agtttaaaat aatcagcaaa aaacaattgg tcaannnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctggtc ttcaatctgt ttttctcagg
     7741 aaaaaacagc gaacattcaa attttcacct cggaatttga acgaattgaa atagttcgcc
     7801 tgcgctctgg aatggattta ttagaaggat taaaggaagc cgttagcagt cgaaaaatca
     7861 aaaatggcgt gatcttagcc ggaatcggcg ccgtgaccga ttatcattac cacgttgtta
     7921 gcagtaaaaa tctaccacca gacgaagagt atccaaaagc ctcagtgcca atggatctaa
     7981 tttccgtgca aggttatatt ctgaatggac gggttcatgc tcatatcgca ctgtcggacg
     8041 caaacagtgt cgttggcggt catctcgaat caggcacaaa agctctgact tttttcatta
     8101 ttactatcgg ggtgttgcct gatagtttaa agatcgacaa tcttgataat tacaaatatt
     8161 aatccgaatg aaaataaata ttcgttcgga aaattcagac caccttttgt ctgattccga
     8221 ccaattttaa cgataaacaa tcagtctttt atttcttcgg cttatcagct gatttactca
     8281 ccagctcgac cataaccacc gatttggctg gcagattaac tgaaagagta ttttgggcta
     8341 ttttgcaatc ggtgaatttt tgcagattga cttcctccgg tttaccaaaa tcattgaagg
     8401 cgttcatctt accagctgtg ataattgacc cactgcttac cgaaaatttg tttttactct
     8461 ttaattcaca ggtcagattc atgtttttat tcggatcgag attgcatagc gtaatatgaa
     8521 taaccccctg tttatcaacg gacgccgaag cattcaccgc cggaattgaa ccgccgtcta
     8581 gttcataatt ctggcaggtc aattcgatcg gtaataaagt tgcatcctga tgcactttgt
     8641 acattttata aacataataa gtgggcgtca agacaatctg gtcttcattc gtcaaaatta
     8701 ttgcctgtaa aacattgacc atttgggcaa tgttagccat tttcactcgt tcgcagtggt
     8761 tgttaaaaat gttcaggtta gtggcggcaa ccagagcatc tctgagtgta ttttgttgat
     8821 atagaaaagc cggattagtg ccgggttcga catcgtgcca attaccccat tcatcaacga
     8881 tgagtccgat tttcttttcc ggatcatatt tatccataat ggcagagtgt ttggtgacaa
     8941 actcatccat tcggagcgtt ttcgacatcg tcagaaacca gtctttttcg ttaaaattgg
     9001 tagcagaacc tttattggtc cagtcatgac aaaccgtata ataatgcaaa gaaattccct
     9061 gaaagcaatt ccgattgatc ggttccttca ttagggtttc catccattcg tagttttcgt
     9121 cagatgggcc acaggctact cgatataact cattgccgct gtaattctta caataagttg
     9181 aaaaacgccg catttcatcg gcgtagaaat ccgccgtcat attgccacca cagccccagt
     9241 tttcatttcc caatccccaa aatctaacgc gccagggttt ttcgcgaccg ttctttttgc
     9301 gcagttcggt catgggacta atattatcag aagtgagata ctcgacccat tgagccattt
     9361 cacgcacggt gccactgcct acattaccgg taattaccgg ttcacagccg acctgctcgc
     9421 agaagtccat aaattcatgc gtaccgaagc tattatcttc ggtaacgccg ccccagtggg
     9481 tattgatcat tttcggtctt tggtcgcggg gaccaatgcc atccatccag tgatattcat
     9541 cggcgaaaca accacctggc cagcgcacat tcggcgcggc aatgtctttc agagcggcga
     9601 cgacgtcatt ccgaatgccc cgagtattgg gaattggtga atcttcccca acccaaattc
     9661 cgccgtaaat acaccgacca agatgttcag cgaaatggcc atagatgtgc cgactgatca
     9721 tatgttggcc ggcctcgaca tcaatgacaa tcttattttc gacgttctgg gcgtacaaat
     9781 tctgcataag aaatataccc aaaatgccga tgactaactt tttcataacg atactccttt
     9841 acatttggtt ataaatttat ttattctttt cttctttgca aattttagcc gcccagtcct
     9901 tgatagaagg attattcccg tctggaaaca gtggcacttt caaatttccc tttttatccg
     9961 cttggattgt ctgagcctct ttccattcac cattgcgcgg atcaaaccac ttaaaatggt
    10021 acattgtatt cggtttaaag ccggacaaag tgggcaaaac cgactgattt tcgaagtaaa
    10081 gcatagccag gtttttatcc ggggttcgca tcatataagc ccagccatct aaacctgttg
    10141 gcggactgtt aggcgcttta ttagggtgaa tatcctcacg ggcaagccgt aaatcctggt
    10201 atttatttcc ctccgataaa ataaatgaac tcaagtgttt catataattt gccgattcaa
    10261 atttcaaggc ttcccaaata tgatgacgtt tcccgac
//
LOCUS       DAOOMU010000079         9803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_8001_length_9803_cov_16.160238, whole genome shotgun sequence.
ACCESSION   DAOOMU010000079 DAOOMU010000000
VERSION     DAOOMU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9803
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8001_length_9803_cov_16.160238"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..974
                     /gene="obgE"
                     /locus_tag="PLI55_07855"
     CDS             3..974
                     /gene="obgE"
                     /locus_tag="PLI55_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPB00789.1"
                     /translation="MFVDYTKVSVQSGSGGKGCVAFRREKFVPKGGPSGGDGGRGGDI
                     IVKVDHNLNTLQDIKYHKSYKAQNGDPGQGGLKSGADGAPIIIRVPKGTLIRDELTNE
                     ILADLVEDNQEVVIARGGKGGRGNAHFATPTKQTPRYAEPGLPGEKKSLIFELKLFSD
                     VGLVGLPNAGKSTLISKITAARPKIADYPFTTLTPNLGIVKFGDYKSFLVADIPGLIE
                     GAHQGKGLGFRFLRHLERTKVLAFLIEATDPEPERTYQTLNTELVKYLSAFENKPRFI
                     VITKIDLVQELPTLKFDNLPIVPISAVTGEGLELFIQQSLKLLAQNG"
     gene            967..2064
                     /gene="dprA"
                     /locus_tag="PLI55_07860"
     CDS             967..2064
                     /gene="dprA"
                     /locus_tag="PLI55_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPB00790.1"
                     /translation="MDKLQAILSLMSINGLGSHRILSLYRQYPDIQEVFSVSPKRLIE
                     VPGIDLKIAERIKNNVDHQFVDQQLNWLKNTPYKITTIIDEDYPVRLRNIYDPPVVLF
                     RYGEFLPQDEDAIAIVGTRNPDHYGKEVAALLVSQLVEQNITIVSGFARGIDTIAHKT
                     ALEEGGRTIAVLGNGIERVYPPENRSLRDKLVTQGVYCSEFPFGTKPDAVNFPRRNRI
                     ISGLSLGTVVISAGERSGALLTAYYALDQNREVFAVPGRITDEKSFGTNHLIQKGAKL
                     VTSVGDILDEIDAIRRFPRQPRQLEISFEFENEDEKAIYEALSTDPIHIDDLAQKLGK
                     NTYSILATLLSLELKGAVRQLAGKMFIKAGL"
     gene            2065..3765
                     /locus_tag="PLI55_07865"
     CDS             2065..3765
                     /locus_tag="PLI55_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00791.1"
                     /translation="MQIFKEIRGNFGLIFSICISILFLPQKSPSQDISDRPVRLYQLW
                     DWISYKYSNYPTSLTEGVELVYFGTSGGIYAFHKYGRYWTEPFTTSNGLSDDFITAVL
                     YDETTRYLWAAHNKGVSYLTPSSFRWTNISNADLNLPETNRILRLGTDGNAIWVQAEG
                     DYLLTINKILGFYQAEEESASEAVKWQPTSLDGVPDMRGYTIDQDYQIDGRGLILDQN
                     LREYRVTVFAKINNDIYGGIAGLGLFEGNANIKTLHIHPFGPMQNYINAMAVDDNRIW
                     VGGTRSDLNPIFTKSGISCYNQNDGFWEYFESLFINEFATPVVNDLAYKNNRLWIGTE
                     QGLSIYDQNKNRWKRLSMSSGLADEIITSIALEDTLAWIGTPRGLNLISIPSYKARRQ
                     RLSPRQNILKIFKIAIDSKNIWIGTDNGIYSIDKFNHNVEHYNMFGERIGLDDAVAGN
                     ITAIGCGDSVTVFGRYHDLLQFSYKDKQWTNIPLTEQLNEASFFDIAIDGNYMWLGTD
                     RGAFLMRLSDYYCENYTTLDGLAGNNVFKIFIDGDWVWFATDCGLTKYSWRKYVTLTE
                     "
     gene            3746..5017
                     /locus_tag="PLI55_07870"
     CDS             3746..5017
                     /locus_tag="PLI55_07870"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPB00792.1"
                     /translation="MSHSLSKTSLLVCGLLTVALAQEPADFRGNVEPEIPNFIYDVSI
                     TPIVTRDSIKIDIIIKVPYSAIQFVKKDTIFIGKYEISVMLLDEKEVNAISKIWTKTL
                     QTKKFEETHSNELYDINKLTYKVKPSDYSLTIGVMDLDTRKTTHRKRELKLKNYYKKS
                     ITLSSINIIEDIVADSSGDSTDVSALTGTLSNKSEKFDISFFVLSNGGKGTIKYSIYN
                     MNKKVVLENKIDHNFKKGVEYQRISIPRQKLSYSKYRLVLTVKIGSEEVTAEKIIQIR
                     WTGMSDMINNLDEAIEQLRYIARGDVISKMKKASGEEKKNLFLEFWRERDPTPGTEEN
                     ELMNEYYRRVAFANQNFSGYLEGWKTDMGMVYILFGPPNDIERHPFEIQTKPYEVWYY
                     YELNRTFIFVDETGFGEYRLITPLDYYGSFY"
     gene            5017..6096
                     /locus_tag="PLI55_07875"
     CDS             5017..6096
                     /locus_tag="PLI55_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPB00793.1"
                     /translation="MLKSRKIAVIAGDGIGPEIIRQVVKIFEVLKNYSQINLAITHAP
                     YSAEYYLNTKVAIPDEYIAELEKNYDAILIGPFGDPRLPDGRYAREIIWNIRNKLDLF
                     FGYQHVKVYDSWMSPLKNTQEGLADFYIIRENLESINPKPGGSYYRDTDKEIAIQSSI
                     YTRQNIDRFIQQAFEFAGKRNYKKVVLAHKNTLMTNLHELWLKIFQEKITAFPEINAD
                     VMNIDALLFELLSDPAKFEIIVAPGDLADSIYTAGLFLQGSYGMAHICEINPVKIGAF
                     RIAQGSAVKIAGHNRANPLGAFIATIEMLKFLELDEAAEAVERALIQILQKHLVTIDM
                     GGLIGTEELGNYFCEFLKEQLSISR"
     gene            complement(6099..6509)
                     /locus_tag="PLI55_07880"
     CDS             complement(6099..6509)
                     /locus_tag="PLI55_07880"
                     /inference="COORDINATES: protein motif:HMM:NF022443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HPB00794.1"
                     /translation="MTLRQLFKRKYAIKILIILIIVPIGFYTKFYSGPFQKWVSYSLG
                     DFFYEIFWCLVISLFFPLLKSSRIAVIVFFATSILEFIQLWHLPILEKIRETFIGRTL
                     IGNYFIWSDFIYYAAGCLAGYFILEAISRVKTAK"
     gene            6715..8145
                     /gene="gatB"
                     /locus_tag="PLI55_07885"
     CDS             6715..8145
                     /gene="gatB"
                     /locus_tag="PLI55_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPB00795.1"
                     /translation="MSEKFEPVIGLEVHAQLLTDTKIFCSCKNAYGAPPNSLTCPVCL
                     GMPGTLPVLNRKAVEYAIKMGLATDCSITTFSRFARKNYFYPDLPKGYQISQYDEPLC
                     RNGKIKIQVNGVEKNIGITRIHLEEDAGKSIHGFDNTLVDFNRCGVALIEIVSEPDLR
                     SPEEAYAYLIKLKQILEYLEICDCNMEEGSLRCDANISLRPIGEEKFGTKTEMKNMNS
                     FRGVERALRFEIERQKAILEKGKNIIQQTMLWDEAKGEARPMRSKEESHDYRYFPEPD
                     LVPLEVDENWKTKISNDLPELPDQKYFRFLNKYNLRHYDAAILTSDKYLAEYFERVND
                     LINDPQLVSKWIQGEVLRTLSQKNLTPKQIPLAAENLGELLNLIKAGTISANIAKEVF
                     DKMVENNESAKIIVEREKLIQVSDDSELEQIIQTVISENPNEVERYRRGAKNLFAFFM
                     GQVMKKTKGKANPSVANNLLRKALDN"
     assembly_gap    8328..8427
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8430..8517)
                     /locus_tag="PLI55_07890"
     tRNA            complement(8430..8517)
                     /locus_tag="PLI55_07890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8481..8483),aa:Ser,seq:gga)
     gene            8646..8978
                     /gene="cutA"
                     /locus_tag="PLI55_07895"
     CDS             8646..8978
                     /gene="cutA"
                     /locus_tag="PLI55_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HPB00796.1"
                     /translation="MKKDAFLQIITTTDSKEKAERIAFILVEKGLAACVQVIGPVTSI
                     YRWEGKIENTIEWICQIKTIKDLYNSVEKEILTHHKYELPEIIAIPILTGLKKYLDWM
                     RMQVTTND"
     gene            9286..>9803
                     /locus_tag="PLI55_07900"
     CDS             9286..>9803
                     /locus_tag="PLI55_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HPB00797.1"
                     /translation="MTSKEFFKFAKQNDAKMVDLKFTDLLGTWQHCTFPINSWGENTF
                     VEGVGFDGSSIRGWQGIHLSDMLAIPDPDTAVMDRFFKEPTVSVIADIIDPITRENYS
                     RDPRFVARKGEAYLKKTGIADTCYIGPEPEFFIFDEVMYEQNQHTSMYLINSIEGAWN
                     TGRFEEPNLGYKP"
BASE COUNT         3109 a         1752 c         2038 g         2804 t
ORIGIN      
        1 atttgttcgt agattacacc aaagtatctg tgcaatccgg tagcggtggt aagggctgtg
       61 tggcttttcg tcgtgaaaag ttcgttccaa aaggcggtcc cagtggcggt gacggtggac
      121 gcggtggcga tataattgtt aaagttgatc ataatcttaa tactcttcag gatattaaat
      181 atcataaaag ttataaagcc caaaatggcg accccggtca aggcggttta aaaagcggcg
      241 ccgacggtgc gccgattata attcgtgtgc caaaagggac cttgatacgt gatgaattaa
      301 ctaatgagat tctggctgat ctggtcgaag ataatcagga agtagtcata gcccgcggtg
      361 gaaaaggcgg acgaggaaat gcccatttcg ccacgccaac gaaacaaact cctcgttatg
      421 ccgagccggg tttgcccggt gagaaaaaaa gcctgatttt cgaactaaaa cttttctcgg
      481 acgtgggatt agtaggtttg cctaatgctg gtaaatcaac tttaatctcg aaaattactg
      541 ccgctcgtcc caaaatcgcc gattatccct ttactactct gactcccaat cttggcattg
      601 tcaaatttgg tgattataaa agtttcttgg ttgccgatat tcccggttta atcgaaggcg
      661 ctcatcaggg aaaggggctg ggttttcgat tcctcagaca tctggaacgc acgaaggttc
      721 tggctttttt aatcgaagca acagatcctg agcccgaacg aacttatcaa acgcttaata
      781 ccgaattggt aaaatactta tccgcttttg aaaacaaacc acgttttatc gtaattacta
      841 aaattgattt ggtgcaagaa cttcccaccc taaaattcga taatttgccg atcgttccca
      901 tctctgccgt tactggtgaa ggactggagt tatttatcca acaatcgctt aaattactag
      961 cgcaaaatgg ataaactgca agccattctc agtttaatga gtattaatgg attgggttct
     1021 catcgcatat tgagcctata ccggcaatac cctgatattc aggaagtatt cagcgtttca
     1081 cctaaacgat tgattgaggt accgggcatt gatttaaaaa ttgcggaacg aataaaaaat
     1141 aatgttgatc accaatttgt cgatcaacaa ttaaactggc taaaaaatac cccttataaa
     1201 atcacgacta taattgacga ggattatcca gttcgattga gaaatatcta cgatcctccg
     1261 gtggttttat tccgatacgg tgaatttttg ccgcaggacg aagatgcgat tgccattgtg
     1321 ggaacccgaa atccggatca ttacggcaaa gaagtagcgg ctctattagt cagccaatta
     1381 gttgagcaga atataacgat agttagcgga tttgcacgcg gaattgacac catcgcccac
     1441 aaaactgctc tggaagaagg tgggcgaact atcgcggttc tgggtaacgg catcgaacga
     1501 gtttatccgc cggaaaatcg gtcattgcgt gacaagttgg ttacacaagg cgtctattgt
     1561 tcggaatttc ctttcggtac caaaccggac gcggtcaatt ttccacgtcg caaccggatt
     1621 atctctggat taagtttagg aactgtcgtt atttctgccg gagaaaggag tggcgcctta
     1681 ttaactgctt attatgcact tgatcaaaat cgcgaggttt ttgctgttcc cgggagaatt
     1741 accgacgaaa agagttttgg caccaatcac ctgattcaaa aaggagccaa attagtcacc
     1801 tcggtcggcg atatattgga tgaaattgat gctattcgtc gcttcccccg tcagccgcgc
     1861 cagcttgaaa ttagctttga atttgaaaat gaagacgaaa aagcgattta cgaagcgctt
     1921 tcgaccgatc cgattcacat agatgatctg gctcaaaaac tcggtaaaaa tacatattcg
     1981 attttggcga ctttgctctc gcttgaatta aagggtgctg tgcggcaatt ggcggggaaa
     2041 atgttcatta aagcaggatt ataaatgcaa atatttaagg aaattagggg caatttcgga
     2101 ttaatttttt caatctgtat ttcgattttg ttcctcccgc agaaaagtcc atctcaagat
     2161 atctctgacc gaccggttcg actttatcaa ttgtgggact ggatttcata taaatatagc
     2221 aattatccga cgagcttaac cgaaggggta gaacttgtct atttcggaac cagtggcggt
     2281 atttacgctt ttcataaata cgggcgctac tggactgagc cgtttactac cagcaatggt
     2341 ttgagcgatg actttataac ggcagtcctt tatgatgaaa caacgcgtta tttatgggcg
     2401 gcacataata aaggcgtcag ctatttaact cccagcagtt ttcgctggac taatatttca
     2461 aacgccgatt taaatttacc ggaaacgaac cggattcttc gactcggtac ggatggtaac
     2521 gcaatttggg ttcaggctga aggtgattat ctcctcacta tcaataagat attgggattt
     2581 tatcaagcag aggaagaatc agcctcagag gcagttaaat ggcaaccgac ttcacttgac
     2641 ggcgtaccag atatgagggg ttataccata gatcaggatt accaaattga tgggcgtggt
     2701 ttaattttag atcagaacct gcgagaatac agggtaacgg tttttgccaa aataaacaat
     2761 gacatttatg gtggaattgc cggattggga ctttttgaag gcaacgccaa tattaaaact
     2821 ctccatatcc atcctttcgg tccgatgcaa aattacatta atgctatggc ggtggatgat
     2881 aatcggattt gggttggagg tacccggagt gatttaaacc caattttcac caaatcagga
     2941 atttcgtgtt ataatcaaaa tgatggtttc tgggaatatt ttgagagtct gtttatcaat
     3001 gaatttgcaa cgccggtagt caatgaccta gcctataaaa ataatcggct ctggattggc
     3061 accgagcagg gtttgtcaat ctacgatcaa aataaaaacc gctggaagcg attatctatg
     3121 tccagtggct tagccgatga aatcattact tcgattgcgc tcgaagatac tctcgcctgg
     3181 attggtacac cgcgcggact gaatctcatc agtattccga gttataaagc gcgtcgtcaa
     3241 cgtttgtcgc ctcgccagaa tattttaaag atctttaaaa ttgccataga ttcaaaaaat
     3301 atttggattg gcaccgataa tggaatttac tctattgaca aattcaatca taatgtcgaa
     3361 cattataata tgtttggcga acgaataggt ttagatgatg cagtagccgg taatatcacg
     3421 gctattggat gcggagattc ggtgacggtt tttggacgtt atcacgatct tctgcaattt
     3481 tcatataaag acaaacaatg gactaatatt ccactgacag aacagttaaa tgaagccagt
     3541 tttttcgata tcgcaattga tgggaattat atgtggctgg gaaccgatcg gggcgcattt
     3601 ctgatgcgtc tatctgatta ttattgcgag aattatacta ctctcgatgg cttggctggt
     3661 aataacgtct ttaaaatttt catagatggc gattgggtct ggtttgccac cgattgcggt
     3721 ctgacaaaat atagctggag aaaatatgtc acactcactg agtaaaacat cgttgttagt
     3781 ttgtgggtta ttaacggtgg ctcttgccca agagccggct gatttcagag gaaacgtgga
     3841 accggaaatt ccgaacttta tctatgatgt tagtatcacg cctattgtga ccagagatag
     3901 tattaaaatt gatataatta ttaaagtgcc ttacagtgca atccaatttg tcaaaaagga
     3961 tacaatattc atcggcaaat atgaaatatc agtgatgcta ctggatgaga aagaagttaa
     4021 tgctatcagt aaaatctgga ctaaaaccct tcaaaccaag aagttcgagg aaactcattc
     4081 taatgaactt tacgatatta ataaactaac ctataaagta aagccgagtg attattcgtt
     4141 aacgattgga gtgatggatt tagatactcg aaagactact catcgcaaac gggaattaaa
     4201 attaaagaat tactataaaa aatcaattac tctcagcagt atcaacataa tcgaagacat
     4261 tgtcgctgac agtagtggag atagtactga tgtttccgca ttaaccggta cactaagcaa
     4321 taaaagcgaa aaattcgaca tctcgttttt cgtattatct aatggaggga agggaacgat
     4381 taaatatagc atttataata tgaacaaaaa ggtagtcctt gaaaacaaaa ttgatcataa
     4441 ttttaagaaa ggagtagaat accagagaat cagtattccg cgtcagaaat tgtcttacag
     4501 taaatatcgg ttggttctta cggtgaaaat cggttctgaa gaagtcacgg ctgaaaaaat
     4561 aatccaaatc cgctggactg gtatgtctga tatgatcaat aatctcgatg aggcaatcga
     4621 acaactcaga tatattgccc gtggtgatgt tattagtaag atgaaaaaag ccagtggaga
     4681 agaaaaaaag aacctattcc ttgaattttg gagggagaga gacccaactc ccggaacaga
     4741 agaaaatgaa ttaatgaatg aatattaccg ccgcgttgct tttgctaacc aaaatttcag
     4801 cggttattta gaaggctgga aaaccgatat gggaatggtt tatatcttgt ttggtccacc
     4861 aaatgatatc gaacgccatc cttttgaaat tcagacaaaa ccgtatgaag tctggtatta
     4921 ctacgaatta aatcggacgt ttattttcgt tgacgagacc ggttttggag aataccgtct
     4981 gattacgccc ttggactatt acggtagttt ttattaatgc taaaatcgcg caaaattgcc
     5041 gtcatcgccg gagatggaat cggtccggaa attatcagac aagtagtcaa aatatttgaa
     5101 gtgctgaaaa actatagcca gattaatctg gcaattacgc atgcgcctta ctcggcggaa
     5161 tattatctga ataccaaagt tgcaattcca gatgaatata ttgccgaact cgagaaaaat
     5221 tacgatgcaa tcttgatagg accattcgga gatccacgcc tccctgacgg tcgttatgca
     5281 cgtgaaatca tttggaatat tcgcaataaa cttgatttat tttttggtta tcagcatgtc
     5341 aaggtttatg attcttggat gagcccatta aagaatacgc aagaaggatt agctgatttt
     5401 tatataattc gcgaaaatct ggaaagcatt aatcccaaac cgggtggtag ttactatcgc
     5461 gatacagata aggaaatcgc gatccaatca tcaatttata ctcgccagaa tattgatcga
     5521 tttattcaac aggcatttga attcgccggg aagcgaaatt ataaaaaagt cgtgctggct
     5581 cataaaaata ctttaatgac caacttgcac gaattatggt taaaaatttt tcaggaaaaa
     5641 attacggctt tccccgagat aaatgctgat gtgatgaata ttgatgctct tttatttgaa
     5701 ctgcttagcg atcccgccaa atttgaaata atagttgccc caggagactt agctgattcg
     5761 atttatactg ccggattatt tttacaaggc agttatggaa tggctcatat ttgtgaaatt
     5821 aatcctgtga aaattggcgc atttcgcatt gcgcaaggat cggcagtcaa aatagcggga
     5881 cacaatcgcg ccaatccttt gggagcattc attgccacga tagagatgct gaaatttctg
     5941 gaactcgatg aagcggctga agccgtagaa cgagcgctta ttcaaatcct acagaaacac
     6001 ttagttacga ttgatatggg aggcttgatt ggcacagaag aactgggaaa ttatttttgt
     6061 gaatttctta aagaacaatt atctatttcc cgttaaaatt actttgccgt cttgactcgg
     6121 ctaatggctt ctaagataaa atatccagct aagcatcctg cggcatagta tataaaatcg
     6181 ctccagataa agtaatttcc gattaaagtt cgccctataa aagtttctct tatcttttct
     6241 aaaatgggta aatgccataa ttgtatgaat tcgagaatac tggtagcgaa aaaaactatt
     6301 actgcaattc gagaagattt caagagcgga aaaaataacg agattaccaa acaccagaat
     6361 atttcataaa aaaaatcacc taaagagtaa ctaacccatt tttgaaaagg tccggagtag
     6421 aattttgtgt aaaaaccaat tgggacaata atcagtataa tcaagatttt aatagcatac
     6481 ttgcgtttaa acaattgtct taaagtcatt gatattcagc attatttagt aagttggata
     6541 aatcattcgt aattatacaa gtacaaaatt atttgggtgg aaattaaaaa gacgggagca
     6601 ggattcaaaa ttaatctccg tcaaattctt aattgaggcg attcataatt tgtctttttg
     6661 ccaaattact atcaaattac gacgcaatgt gttcgttaaa aagaaggtcc gaaaatgtcc
     6721 gaaaaatttg aaccagtcat cggcttagaa gtccatgccc aacttctgac agatacgaaa
     6781 atattctgca gttgtaagaa tgcctatggc gcaccgccta attcactgac ctgtccggtt
     6841 tgtctgggaa tgcccggcac tctaccggtt cttaatcgga aagccgttga atatgccatt
     6901 aaaatgggtc tggcgacgga ttgttcgatt actacttttt cgcgttttgc ccgtaaaaat
     6961 tacttttatc cggatttgcc gaaaggctat caaatttcac aatatgacga gccgctgtgc
     7021 cgaaatggaa agattaaaat ccaagtaaat ggagtagaaa aaaatattgg tataacacga
     7081 atacacctcg aagaagatgc cggcaaatct attcatggtt ttgataatac tctggttgat
     7141 ttcaatcgtt gtggtgtagc gctaatcgag attgtctctg aaccggactt gcgttcaccg
     7201 gaagaagctt acgcttatct tattaagttg aagcaaatcc ttgaatattt agaaatttgt
     7261 gattgtaata tggaagaagg cagtttgaga tgtgatgcca atatttccct gcgaccgatc
     7321 ggagaggaaa aattcggcac taaaaccgaa atgaagaata tgaattcatt ccgtggagtc
     7381 gaacgagcct taagatttga aattgagaga caaaaagcta ttttagaaaa agggaaaaac
     7441 attattcagc aaacgatgct ctgggatgaa gccaaaggcg aagcccgccc tatgcgttct
     7501 aaagaagagt ctcatgatta tagatatttt ccggaacccg atcttgtccc acttgaagta
     7561 gacgaaaatt ggaagacgaa gatatctaat gatttaccgg agttgcctga tcagaaatat
     7621 tttcgtttct taaataaata taatctgcgt cattatgatg cggctatatt aacaagtgat
     7681 aaatatctgg ctgaatattt tgagagggta aatgatttaa ttaacgatcc tcagctggtc
     7741 agcaagtgga tacaaggcga agtgttacgt actttatcgc agaaaaatct taccccgaaa
     7801 cagattcctc tcgcggcgga aaaccttggt gaattactta atctgataaa agccggtacg
     7861 atttcagcca atattgctaa agaagtcttc gacaaaatgg tggaaaataa tgaatcggcc
     7921 aaaatcatcg tcgagcgtga aaaattgatt caagttagtg atgattccga actggaacag
     7981 atcattcaga cggtaatttc tgagaatcct aacgaagtcg agcgttatcg cagaggagct
     8041 aaaaaccttt ttgctttttt tatgggacaa gtaatgaaga aaacgaaagg taaagctaat
     8101 ccaagcgtcg ccaataatct gctgaggaaa gccctggata attgatgctt tatatcttaa
     8161 ttattgaagt ttcgaatatc aaaattaatt gataataaat aacaattttc ataataactc
     8221 tgtttgaaga ataaacattg ttaatcccgt cagaaataat ctgcggagag acggggattc
     8281 cctgcctgac tgctgacgca gtcacgcagg cagggaaccc cgggtggnnn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnntgc ggagagacgg ggattcgaac cccgggtagg
     8461 gtataagcct acacacgctt tccaagcgtg ctccttaaac cactcggaca tctctccaaa
     8521 aagcgccata gtttaaatta attgaaccaa taagacaaga aaaaccaaga gactgattat
     8581 atggtagatt atcattttag tatttaatat ttaaattatt ttatgattat taggagtgtt
     8641 aaaaaatgaa aaaagatgct tttttacaaa ttattaccac caccgattcg aaagaaaaag
     8701 ctgaaagaat cgcatttatt ctggtagaaa aaggtttagc cgcctgcgtt caggttatcg
     8761 gtccggtaac cagtatctat cgctgggaag ggaaaataga aaataccatt gaatggatat
     8821 gccagataaa aactatcaaa gatttgtaca attctgtcga aaaggaaata ctcactcatc
     8881 ataaatacga attgccagaa ataattgcca taccaatttt gacaggattg aaaaaatatt
     8941 tggactggat gcggatgcaa gtcacaacaa acgattgaat taacactttt tctctggtca
     9001 ttctgataat aatctgcttc ttgggcagtt cattacttta ataattaaaa gcgatttgaa
     9061 gtactaatcg taactgctac attagtaatt aataatatta agaacattag ccttgtaata
     9121 tttctaataa ttaataatgt aaagataaat ttaaaattgg cataatatat atgtattttc
     9181 tattaattaa taaaataatt tgtatgaatt tacttgaata cttaatatag agtcacattt
     9241 tttgggttaa tttataagta taacaacatt aaaaaggaat cttatatgac atcaaaagaa
     9301 ttttttaaat ttgccaaaca aaatgatgct aaaatggtgg atttgaagtt tactgacctg
     9361 cttggcacgt ggcaacattg tacttttccg atcaattctt ggggtgaaaa tacttttgtc
     9421 gaaggcgttg gatttgacgg ctcttcgatt cgtggttggc aggggattca tttgtcagat
     9481 atgctggcta ttcctgatcc agatacagct gtgatggatc gtttttttaa agaaccaact
     9541 gttagtgtta tagctgatat cattgaccca atcactcgag agaattatag ccgcgatcca
     9601 cggtttgtcg cccggaaagg cgaggcatat ttgaaaaaaa ctggcattgc cgatacctgc
     9661 tatattggac cggagccgga attcttcatt tttgatgaag taatgtatga acaaaatcaa
     9721 cataccagta tgtatctgat caattccatc gagggtgcct ggaataccgg acggtttgag
     9781 gaacctaatc tgggatataa acc
//
LOCUS       DAOOMU010000080         9802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_8003_length_9802_cov_20.219760, whole genome shotgun sequence.
ACCESSION   DAOOMU010000080 DAOOMU010000000
VERSION     DAOOMU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8003_length_9802_cov_20.219760"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..2771)
                     /locus_tag="PLI55_07905"
     CDS             complement(132..2771)
                     /locus_tag="PLI55_07905"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPB00798.1"
                     /translation="MKKFKVVANLCFLTICLSGITMGNTQVKATRTDESIRIDGILSE
                     EIWQRAIPAPPLIQDEPNEGAQPSQPSDIWIVYDDEALYVGARLYDSAPDSIVARLGR
                     RDSDWNTDLFLIALSPFNDKRTAYFFGVSAAGAIYDGKFYNDNWEDDTWDGVWEGAAQ
                     RNHEGWTAEIRIPFSQLRFQKSDQYVWGVALYRKIQRKNEEDMSFFTPRNESGMVSRF
                     IELVGIEGINAERKIEVLPYIRGKGEFLAVEPKDPFNDGSRYFASTGADFKIGVGNNL
                     TVDGTINPDFGQVEVDPAVINLSDVETYYNEKRPFFIEGSDIFNFGYGGSNNYWNMNF
                     STPSIFYSRRIGRAPQRDLSDYDYTDVPDGTRIISAGKLTGKVGDNWNVGLLNALTAR
                     EFARVSPNGLDSKIEAEPLTNYAILRTQKEFQEGRHGLGIITTSTKRFFRDESLADYL
                     NSDALVVGLDGWTFLDTNKTWVIAGWGAMSNIQGNENCITDVQTNARHYFQRPDAEHL
                     GVDSNATALTGFATRINLNKQRGNVIFNAAFGLIDPRFEVSDLGYLSRGDVINYHVGA
                     GYQWTEPNHFYRKFNLILAHTLGMDCGGNLTNNGIMNLGYIQFSNYYWIDYGSGYFWE
                     TYDTRLTRGGPIAISPSGRWGYIQISTDERKPLVGRLYYETSGNDTASVSHYLQIPLD
                     WKVSDNLAFELSPSISINHNYAQYVDVFDDPYADATYGKRYVFAKMDQKTISASLRLD
                     WTFTPRLTLQLYAQPLYGAAEYYDFKEFARPKTYQFFTYEEGTEINRNSDGNYTVDPD
                     GNGPAGSFSFDNPDFNVYSFRSSAVLRWEFALGSAIYLVWTHGRFENSSEADFDLSRS
                     VNRIGKIKPDNIFMIKASYWFNV"
     gene            complement(2916..5612)
                     /locus_tag="PLI55_07910"
     CDS             complement(2916..5612)
                     /locus_tag="PLI55_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB00799.1"
                     /translation="MNTKKRPYEDIAEELVCESEDKLASLVRNLPGAAYRCLYDAERT
                     MIFISDGCRSLTGYSPDDFLMDHKITYSQIIHPNDRQYVFDEIGASLKKRSPYQLLYR
                     IITADNQEKWVCEQGVGIYNGDENLVVLEGFITDISEKVKANIALQSYETRFQSIWEG
                     SFDGMRLTDKDGKIIAVNKAFCRQVGMRRNELENASIGIIYPPEERQEIETNYRNNFS
                     RRHIKSLTECESILWNGQKVWFEISNSFLDIPGEQVYLLSIFRDITKRKNAEAELAES
                     ARRFRWLYEYAPIAYHTITPDGIIVDVNRQWCEIMGYSKEEVCGRPIFDFVIESERES
                     ARKSFDEKKLSKKTSSTGNERHYLTKSGEVKTFSISDYIIRDDDGRIRSIQTTMSDIS
                     ELKRTEEALRMSEAKFRHLVESSLDVIWQADDSGRVLYVSPNIKDILGRTPDKLIGKQ
                     FADILPKGSEEKGGKIIATHFAKRKPIKNLELTIVNHLGQEIILEVNGVPNYDGGKKF
                     TGYIGTMRNVTIQKIVEDEQIKVQKLESLSILAGGIAHDFNNMLSGILGNISLAKMKV
                     KDSDITRILERSEKAALRSRSLTQQLLTFAKGGAPVMESIQVESFLRELVEFAMVGSS
                     VNCQFDIAPDLPNVEADRGQLSQVIQNLVINAIQAMPSGGNLKISANAEEIKTDGLVP
                     IKSGYYLKIAIQDTGVGIPKNLLSKVFDPYFTTKPHGNGLGLSVVYSILKKHDAYIFV
                     DSEAGKGTTFTVYLPATAAVQKSSQKAESQILKGHGRILVMDDDELICEVVEEMISSM
                     GYDVKCVPNGEEAIKSYKEAKEAGQPFDLVIMDLTIVGGMGGKETIAELKKFDPDVKA
                     LVSSGYINDSMMTVPQKYGFIDIIAKPYRMEDLSSILKEHVG"
     gene            complement(6048..6440)
                     /locus_tag="PLI55_07915"
     CDS             complement(6048..6440)
                     /locus_tag="PLI55_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012781133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HPB00800.1"
                     /translation="MTILGNILWIFLGGGILIFFFYLFGGVVLCLTIIGIPFGMQCIK
                     LSVLGLMPFGQKIVYKASEPGCLSTLMNIIWLLFGGLELAVVHLVFAALCAITIIGIP
                     FARQHIKLLSLALTPFGREVQPIQKDRT"
     gene            complement(6579..7010)
                     /locus_tag="PLI55_07920"
     CDS             complement(6579..7010)
                     /locus_tag="PLI55_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HPB00801.1"
                     /translation="MSINETKIVVCPHCQHEQSVKIWQSLNTALDKEVKKELFEGKIN
                     LLVCENCQQKTFIPVPFLYIDPERQIAVHYFPTAVIRDEKFLQQFNIDGTYAGGESTG
                     LEVPDYMRYLHIVFNMDELVTFIVFREVLFDFHRTGKVLLD"
     gene            complement(7034..9160)
                     /locus_tag="PLI55_07925"
     CDS             complement(7034..9160)
                     /locus_tag="PLI55_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020262735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPB00802.1"
                     /translation="MNFRKQLRTSISAISLALAIWLSGCPSRPLTPPTAKIEPKTTII
                     HSDTLIDNYAWLREKSNPEVIQYLEDENAYTEAIMKPTRRFQKKLYKEMLARIKETDE
                     SVPVKIDSFYYYNRMVKGNQYPLYCRKKGSLDANEEILLDQNRLASGQKYCSIGEFKV
                     SPDHSLLAFAVDFNGSEKHMLVFKDLINGQMLPDTILDVSYGLEWANDNRTVFYTTLD
                     TILRPDKLWRHQLSQSPENDVMVFHEPDEKFYLGIAKTRSHGYLILTLGSQITTEVHY
                     LSADQPDGRFRLFCPRKTGIEYFIDHHSDKFYILTNENAINFRLLETPIARPEKHNWQ
                     EKIPHRPNVMLTGIDLFCDHLVVYERENGLEKIRVENLNTGVMHYVDFPEPVYTIDGD
                     NNPDYNTKILRFRYTSLVTPNSVYDYDMESQTRELKKQDEVLGGYDQTQYQSERIYAV
                     AKDGVRIPISLVYKKGMKKDGSNPFLLYGYGAYGIPSEARFSSIRLSLLDRGFIYAIA
                     HVRGGGELGRQWYEDGKLLRKRNTFTDFIACAEMLIAEKYTSAEKLVINGGSAGGLLM
                     GAVVNMRPELFEVVVAEVPFVDLINTMLDRSIPLTVIEWEEWGNPHLEKYYNYMKSYS
                     PYDNVGPKAYPHMLITAGLNDPRVAYWEPAKWTAKLRATKTDDNILLLKTDMGKGHFS
                     ASGRYDYLKEVAFKYAFIFAKLGIRE"
     gene            complement(9173..9661)
                     /locus_tag="PLI55_07930"
     CDS             complement(9173..9661)
                     /locus_tag="PLI55_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007984056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPB00803.1"
                     /translation="MAQNNAEILIRSARLSDADDLVAFNRAMAQETENRILSAEVISA
                     GVRNLFQHPEWGFYIVAESDGRMVGCLMITYEWSDWRNGLFWWIQSVYVPPEFRRRGI
                     YRKMYDYVQNAALKVGNVCGLRLYVERDNRIAQKTYRKMGMSKTDYILFETTELAKRT
                     NK"
BASE COUNT         2831 a         2282 c         1890 g         2799 t
ORIGIN      
        1 tcgcttcttg tcatcctgag cttgtcgaag gatgacattt ccatctccct atttcaattt
       61 ctcagcgtca gccttagcct tttcattact taatcccata agggcgaact atctggagaa
      121 tctttccagc attagacatt gaaccagtaa gaagccttaa tcataaaaat gttatccggc
      181 ttaatttttc cgatgcgatt gaccgaacgg cttaggtcaa aatctgcctc actactattt
      241 tcaaagcgac catgcgtcca gactaagtaa atcgccgaac cgagcgcaaa ttcccagcgc
      301 aataccgcgc tggagcggaa actatataca ttaaagtcag gattgtcaaa tgaaaaggag
      361 ccggccggac cgttaccgtc cggatcaaca gtgtaattcc catcgctatt tctattaatt
      421 tccgtacctt cttcataagt aaaaaactga taggttttcg gtcgggcaaa ctctttaaaa
      481 tcgtaatatt ccgccgcacc ataaagcggt tgggcatata attgaagtgt caaacgcggg
      541 gtaaaagtcc agtccaagcg caggctggca gatatagtct tttgatccat cttagcaaag
      601 acgtagcgtt taccataagt tgcgtccgca taggggtcat cgaaaacatc cacatattgg
      661 gcataattat gattaataga aatgctggga gacaactcaa aggctaaatt atcagaaact
      721 ttccaatcga gcggaatttg taaataatga ctgaccgagg cggtgtcatt gccgctggtt
      781 tcgtagtaga gcctgcctac caacggtttg cgttcatctg ttgatatttg aatatatccc
      841 caccttccgg atggagatat ggctattgga ccgccgcggg taagtctggt atcataagtt
      901 tcccaaaaat agccgcttcc ataatctatc cagtaataat tcgagaattg aatataaccc
      961 aaattcataa taccattgtt agtcaagtta cctccgcagt ccataccgag tgtgtgagcc
     1021 agaattaaat taaatttgcg atagaagtga ttcggctccg tccactgata accggcaccg
     1081 acgtgataat tgataacatc gcctctcgat aaatatccca ggtcactaac ctcgaaccgt
     1141 gggtcaatta aaccaaatgc ggcattaaaa atgacattgc cacgctgttt attgagatta
     1201 atccgagtcg caaatccagt cagcgcggtg gcattactat ctactcctaa atgctcagca
     1261 tccggtcgtt gaaaataatg acgcgcgttc gtctgaacgt cggtaataca attctcatta
     1321 ccctgaatat tggacatcgc gccccatccg gcaattaccc aggttttatt agtatccaga
     1381 aaagtccagc cgtccagccc cactaccagg gcatccgaat tgagatagtc ggcgagactt
     1441 tcatcccgaa aaaagcgttt ggtagaagtg gtaattattc ccagcccgtg gcgaccctct
     1501 tgaaattctt tctgcgtccg cagaatcgca taattagtca gcggctcagc ttcgattttc
     1561 gagtcgagac cgttgggact aacgcgggcg aactcacgcg ctgtcagggc gttcaatagc
     1621 cccacattcc agttatcgcc aactttgccc gtcaattttc cggcggagat gatgcgtgtt
     1681 ccgtccggca catcggtata atcgtaatcc gacaggtcgc gctggggggc gcgtccgatg
     1741 cgccggctat aaaatatgct gggcgtcgaa aaattcatat tccaataatt attggaacca
     1801 ccataaccga aattaaaaat atccgatcct tcgataaaga acgggcgctt ttcattataa
     1861 taggtttcga cgtcggacag attaatcact gctggatcaa cttccacttg accaaaatca
     1921 gggttaatgg taccgtcaac ggtcagatta ttacccaccc caatcttgaa atcggcaccg
     1981 gtactggcaa aatagcgtga accgtcgtta aaagggtctt tcggttcaac tgccagaaat
     2041 tcacccttgc ctcgaatata gggcaaaacc tcgattttgc gttccgcatt aatgccctcg
     2101 atgcccacca gctcgatgaa acgcgaaacc attcctgatt cattgcgggg cgtaaaaaag
     2161 ctcatatcct cctcattctt acgttgaatt ttacgataca acgccactcc ccacacatat
     2221 tgatcgcttt tctggaaacg caattgtgaa aaagggatgc gtatttcagc cgtccaacct
     2281 tcatgattac gttgtgcggc gccttcccaa acaccgtccc aggtatcatc ttcccaatta
     2341 tcattataaa attttccgtc gtaaatcgct cctgcggctg aaaccccaaa aaaataggcg
     2401 gtgcgtttgt cattgaaagg actcaaggcg atcagaaaga gatcggtgtt ccagtcggaa
     2461 tcgcgtcggc caagccgcgc tacaattgaa tctggggctg aatcataaag ccgggcgccg
     2521 acgtagagcg cctcgtcatc atagacaatc cagatatccg atggctggct tggttgagca
     2581 ccttcattcg gttcatcttg aattaaaggt ggcgccggaa tagctctttg ccagatttcc
     2641 tcacttaaaa ttccgtcaat acgaatagat tcgtccgttc gagtcgcctt tacctgagta
     2701 ttgcccattg ttattccgga caaacatatt gtcagaaagc acagattagc gacaacctta
     2761 aactttttca ctattaaact cccggttaat cattaaaaaa acacaacaac gccctataga
     2821 ttggattaac caggatttag ttgtcctatc cctgagttat tttcaaccca gtagacaata
     2881 atttgtgatt agaatcaaaa gtcggttgaa agttctcagc cgacatgctc ctttaaaatg
     2941 ctactgagat cttccatgcg atatggttta gcgattatat caataaatcc atatttttga
     3001 ggaacagtca tcatcgaatc gtttatataa ccactagaga ctaaagcttt aacatcagga
     3061 tcaaatttct ttagttctgc gatggtctct ttaccaccca tacctccgac aatagtcaaa
     3121 tccataatga ccagatcaaa tggttgcccg gcttccttgg cttccttata agatttgatg
     3181 gcttcttcac cgttcggaac gcatttgaca tcgtagccca tactggaaat catctcctca
     3241 accacttcac aaatcaattc atcatcatcc atcactaaaa ttcggccatg cccctttaaa
     3301 atttgggatt cggctttttg tgaactcttt tgaacagcgg cggttgccgg cagataaacc
     3361 gtaaaagttg tgcccttgcc tgcttcggaa tccacaaaaa tatatgcatc gtgttttttc
     3421 agtatagaat aaacaaccga caagcccaaa ccgtttccgt gaggtttagt ggtaaagtaa
     3481 ggatcaaata ctttggataa aaggttttta ggaataccaa ccccagtatc ttgtatcgca
     3541 attttcaagt aatacccgga tttaattggt acaagaccat ctgtttttat ttcctcagca
     3601 ttagccgaaa tttttaggtt gcctccggac ggcatagcct gaatagcatt tataaccaaa
     3661 ttttgaatca cttggctcag ctggccacga tcagcctcca cattgggcaa atcgggagca
     3721 atgtcaaact ggcagttaac cgacgatccc accatagcaa attcaaccaa ttctctcaga
     3781 aaactctcaa cctggatgga ttccataacc ggcgcaccgc ccttggcaaa agttaacaat
     3841 tgctgagtca gggagcgaga acgtaaagcg gccttctcgg atctttcgag gatccgagtt
     3901 atatcactgt ctttaacttt catctttgcc agtgatatgt tacccaaaat accggaaagc
     3961 atgttattaa aatcatgggc aatgccacca gccaggatac tcagagattc caacttctgc
     4021 accttgatct gctcgtcttc gacaattttt tgaatagtta cattgcgcat tgtaccgata
     4081 tatcctgtaa atttcttacc gccatcataa ttgggtacgc catttacttc aaggataatt
     4141 tcttgaccga ggtgattaac tatagttaac tccaggttct tgatcggctt acgcttggca
     4201 aaatgcgtcg caatgatttt gccacctttt tcttcagaac ctttaggtag aatatcagca
     4261 aactgtttac caataagttt atcaggggta cgtccgagaa tatcttttat attcgggctg
     4321 acatataaaa cccgtccgga atcgtcagcc tgccagataa catccagact ggattctact
     4381 aaatgacgaa atttagcctc gctcatccga agcgcctctt cggtgcgttt aagctcacta
     4441 atgtcggaca tagtggtctg aatggaacga atacgaccat catcgtccct gatgatataa
     4501 tcagaaatag agaaagtctt tacttctcca ctcttagtta aataatgacg ttcattcccc
     4561 gtagaactcg tcttcttgct taatttcttt tcatcaaagg atttacgagc cgattctctt
     4621 tcactttcga taacgaaatc aaagatcggt cgcccacaaa cctcctcttt ggagtaaccc
     4681 ataatttcac accattgacg gttgacatcc acaataatac catcaggagt aatggtgtgg
     4741 taagctattg gtgcatattc atataaccag cgaaatcggc gagcagattc agccagctcg
     4801 gcttcggcat ttttacgctt ggtaatatca cgaaaaatgc tcagtaaata aacttgctca
     4861 ccgggaatat ccagaaaaga attggaaatt tcaaaccaga ctttctgacc attccagaga
     4921 attgattcgc attctgttaa ggatttaata tgtcgtcgag agaaattatt tcggtaattt
     4981 gtttcaatct cctggcgctc ttccggcgga taaataatcc ctatactggc attttccagc
     5041 tcgtttcggc gcatgcctac ctggcgacaa aaggctttgt taacggcgat tattttgccg
     5101 tccttgtccg tcaaccgcat gccatcaaaa ctgccttccc agattgattg gaaacgcgtt
     5161 tcgtaacttt gcagggcaat attggcttta accttttcac ttatatccgt tataaaacct
     5221 tcgagcacta cgagattttc gtctccgtta tagataccga caccttgctc acaaacccac
     5281 ttttcctgat tatcggctgt tataatccgg tatagtaact gatagggact ccgctttttc
     5341 agagaagcac caatttcatc aaatacatat tgccgatcat tcgggtgaat aatctggcta
     5401 taggttattt tatggtccat taaaaaatca tcaggcgaat aaccggttaa tgaacggcaa
     5461 ccatcgctga tgaaaatcat tgtcctttcg gcgtcatata aacaacgata agccgcacct
     5521 ggtaaatttc ttaccaaaga agccaattta tcttccgatt cacaaacaag ttcttctgct
     5581 atatcttcat aaggacgttt cttcgtattc atttaaaccc ctcaaattta tcagaacagc
     5641 tactattcga aaataaccgg cctacacaat gtcgcccaag aactaaatat tgttttattg
     5701 tgtgccgttt tccacatgtg aatactaaaa aataatattt aaaaatgcaa gtcgtttttt
     5761 aggttgaggg taaggcaaag gctaataaga ggacagaaac agaaaccaca cctagctgta
     5821 accgaatatc tattaaacat ttttgattaa tttgaaagcg caatatgttg tataacactt
     5881 tgtaagttaa aatttctgaa aaatagccgt attatagtgt taaaaataaa tttctatcaa
     5941 tggtcaaaaa agcacattgg agatccaatg tattcataag ccagtctaaa gatagatgac
     6001 tatatttggg gataaaaaat taatgatttt ctgcggttat cagacaatca tgttctatcc
     6061 ttctgaatcg gctgaacttc ccgaccaaaa ggagttaatg ccagtgatag caatttgata
     6121 tgctgtcttg caaagggaat accgataata gtgatagcac acaatgcggc gaaaactaaa
     6181 tgcactaccg ccagctctaa accgccaaaa agtaaccaga taatattcat taaagttgac
     6241 aaacagcccg gttcagatgc tttatagact atcttttgtc caaacggcat tagtcccaac
     6301 accgaaagct tgatacattg cataccgaaa ggaataccga tgatggtcag acatagcacc
     6361 acaccgccaa ataaataaaa gaaaaaaatt aggattccgc cacctagaaa aatccacagg
     6421 atattgccta atattgtcat acttactccc atttaaagac taactttaat acaaagaaac
     6481 aggttttacg attggacata gttaagtatc attgcggcta attgccctaa cgtattccac
     6541 cataccttaa aaagcgatct cctggcttaa gctaacactc aatccaacaa caccttgcca
     6601 gtacgatgaa aatcaaacaa tacttctcta aaaacgataa aagtaaccaa ctcatccata
     6661 ttaaatacaa tatgcagata gcgcatatag tctggcacct ccagtccggt gctttcgccg
     6721 ccggcatagg ttccatcaat attgaattgt tgcagaaatt tttcgtcgcg tattacagct
     6781 gtcgggaaat aatggaccgc gatttgcctt tccggatcaa tgtataaaaa cggcactgga
     6841 ataaaggtct tctgctggca attttcacag accaacaggt ttattttccc ttcaaataat
     6901 tcctttttca cttctttgtc aagagctgta ttgagcgatt gccaaatttt tacactttgt
     6961 tcgtgctggc aatgggggca aacgacgatt ttggtttcat taattgacat tggattttcc
     7021 ttttagaatt aaattattcg cgaattccaa gtttagcgaa aataaaggca tatttaaagg
     7081 ctacttcctt taggtaatca tagcgcccgg atgctgaaaa atgacctttg cccatatcgg
     7141 ttttaagaag taaaatgtta tcatctgtct ttgttgcccg cagtttggca gtccacttgg
     7201 caggctccca ataagctacg cgcggatcat tcaatccggc tgtgattagc atatgcggat
     7261 aggctttagg tccaacgtta tcgtacggcg aataggattt catataattg taatactttt
     7321 ccagatgggg attgccccat tcctcccatt caatcaccgt cagcggaatc gaacgatcca
     7381 gcatcgtatt gatcaaatcc acaaacggta cctcagctac tacgacctca aataattccg
     7441 gacgcatatt gactaccgca cccatcaaca agccgccggc gcttccacca ttaattacca
     7501 acttttcggc tgaggtatat ttttcggcaa tcaacatctc ggcgcaggcg atgaaatcgg
     7561 tgaaagtatt acgctttctt aaaagtttac catcttcata ccactgacgt cccagctcgc
     7621 caccgccgcg gacgtgagca attgcataaa taaatccgcg atccagcaga cttagtcgga
     7681 ttgaagaaaa acgcgcttcg ctgggaatcc cataagcacc ataaccgtaa agcaaaaatg
     7741 gatttgaacc gtcttttttc ataccttttt tataaaccag agaaatcgga attcgaactc
     7801 cgtctttggc aacagcataa attctttccg attgatactg cgtctgatcg tagccgccta
     7861 gcacctcatc ttgctttttc aattcacgag tctgactttc catatcataa tcataaactg
     7921 aatttggcgt aactaaagac gtataacgga atcgcaatat tttcgtgttg taatcgggat
     7981 tattatcacc gtcaatggta tagactggtt cggggaaatc tacgtaatgc attacccctg
     8041 tatttaaatt ctcaacccgg attttttcca gaccgttttc tcgttcatat acaaccagat
     8101 ggtcgcagaa aagatcaatg ccagtcagca taacattggg acgatgagga attttctcct
     8161 gccaattgtg tttttcgggt ctggcaattg gagtttccag caaacgaaaa tttatggcat
     8221 tttcattagt taaaatgtaa aacttgtcgc tatgatggtc aataaaatat tctatcccgg
     8281 tcttacgcgg gcagaataat cgaaatcgtc catctggctg gtcagccgat aggtaatgaa
     8341 cttcggttgt aatctgactt cccaaggtta gaatgaggta accgtgactg cgcgtcttag
     8401 ctattccgag ataaaacttc tcatctggtt cgtgaaaaac cataacatca ttttccggcg
     8461 actggctgag ctgatgacgc cagagcttgt ccggccgcag aatcgtatcg agggtcgtat
     8521 aaaaaacagt acggttatcg tttgcccatt ccagtccata ggaaacatca agaattgtat
     8581 ccggcaacat ctggccattg attaggtctt taaaaaccaa catatgcttt tctgaaccat
     8641 tgaagtcaac ggcgaatgcc agcaaactat gatcgggact tactttgaac tctccgattg
     8701 aacagtattt ctgcccgctg gctagccggt tctgatcgag taggatttcc tcattcgcat
     8761 ccagcgaacc tttcttgcgg caataaagcg gatactgatt gccctttacc atgcggttgt
     8821 aatagtaaaa gctatctatt ttgaccggca cggactcatc cgtttcctta atccgggcta
     8881 acatttcctt gtaaagcttt ttctggaagc gtctggtcgg tttcatgatt gcctcagtgt
     8941 aggcattttc atcttcgaga tattggatga cttctggatt agatttttcc cttagccagg
     9001 cataattatc aattagtgta tcactatgaa taatcgttgt tttgggttcg atttttgccg
     9061 tcggcggagt cagcggtcgt gagggacagc cgcttagcca gattgccagg gcgaggctaa
     9121 tagctgatat actcgttctt aattgtttcc taaaattcat attctctcct gattacttat
     9181 tggttcgctt tgccaattcg gtggtttcaa ataaaatata atccgtcttg gacataccca
     9241 ttttgcgata ggttttctga gcaatccggt tatcgcgctc gacgtaaagc cgcaaaccgc
     9301 agacattacc gactttgagg gcggcattct gaacatagtc gtacattttg cggtaaattc
     9361 cgcggcgccg gaactccgga ggcacataca cactctgaat ccaccagaaa agcccattac
     9421 gccaatcgct ccattcatag gttatcatta gacagccaac catccgtccg tcactctccg
     9481 ccacgatata aaaaccccat tccgggtgct ggaatagatt acgcactcct gcagagatta
     9541 cttccgcact taaaatacgg ttttcagtct cttgcgccat cgcccgatta aaagcaacca
     9601 agtcgtccgc atcggataaa cgggcggacc gaatcagaat ttccgcgtta ttttgtgcca
     9661 tctatttttc gccatttgac ggaattaata ctaaacatta tttggatgat atgaaatagt
     9721 tttcgtgttg gcagaaaatt tttatctcag gacggataga gaattcaatt ccaaaatggg
     9781 aatcattaac taaagataca ac
//
LOCUS       DAOOMU010000081         9724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_8103_length_9724_cov_23.744544, whole genome shotgun sequence.
ACCESSION   DAOOMU010000081 DAOOMU010000000
VERSION     DAOOMU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8103_length_9724_cov_23.744544"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1018
                     /gene="aroF"
                     /locus_tag="PLI55_07935"
     CDS             167..1018
                     /gene="aroF"
                     /locus_tag="PLI55_07935"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPB00804.1"
                     /translation="MGNSENLKLTSIESHLNKTTIKIGDVTIGQDFVVIAGPCAVESE
                     EQLMQTAEAVKAAGANMLRGGAFKPRTSPYDFQGLGLKALKLLEKAKKKYGLPIVTEV
                     LDSRDVTWICEYVDILQIGARNMQNFALLKEVGKSSKPVLLKRGMYSTLKEWLNCAEY
                     ILSEGNPNVILCERGIRTFETYTRNTLDLSIVPAVREVTHLPVIVDPSHATGRLGLIK
                     PMSLAAVAAGVDGLIIEVHYKPETALSDRDQQLNPEQFKVLMRDVLSLKNFMQTLSNS
                     NNASHSN"
     gene            999..2036
                     /gene="aroB"
                     /locus_tag="PLI55_07940"
     CDS             999..2036
                     /gene="aroB"
                     /locus_tag="PLI55_07940"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPB00805.1"
                     /translation="MPAIQIKTAGKTSTIITCPSIREVFRLIEGRPVVIITDQNLDRS
                     YGDLFPEFPKIVLSNGESSKSLETVRQIYEQLGTAEVDRDYTILGIGGGVVCDIAGFV
                     ASTYLRGLDFGFVATSLLAQVDASIGGKNGVNFAGAKNIIGTINQPRFVLCPLDVLET
                     LPRAQFINGTAEIIKTGAIGDADLLEMLATQQAQFINLVPDVTEQIIFRTLNFKAGIV
                     MRDEREQGERRKLNFGHTIGHAIEIASGILHGQAVAIGMNIAAEISVRLGLLARSEKE
                     RLEKILTAYELPTATSLPANELLPLIFKDKKRQGEHLRFVALKKLGSAEVINLSFNQI
                     KEFLDDLCCVG"
     gene            2014..2658
                     /locus_tag="PLI55_07945"
     CDS             2014..2658
                     /locus_tag="PLI55_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPB00806.1"
                     /translation="MICVALAELNFPEIMAILKTEEMVEIRIDLLNLTETQIIAIFRS
                     HPNLVATCRKGRYSDEERQALLALAIRNGAAYVDLECDSPEDWRRSLTQLAREHHTRL
                     IISYHNFELTPGLAELHLLLRNCEALPSDIVKIVTYCQTESDAERLLSLYSLNTKPLI
                     AFGMGEAGRKSRLKALRLGAPFIYAAAETGQPTAPGQMTKNEILELLAEESANV"
     gene            2651..>3171
                     /locus_tag="PLI55_07950"
     CDS             2651..>3171
                     /locus_tag="PLI55_07950"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPB00807.1"
                     /translation="MSEKRFAIIGHPILHSKSPQIHNHWFRENNLSANYSRIIANDVY
                     VALGMGYKLGLTGANVTAPFKEKVIPSLVYLSPEAAAVGAVNTLIYNGPNISGYNTDV
                     TGVIRAFQINNVELKHKSLLVIGAGGAARAIVSALKPSGAEITIVNRPDEMALNLARS
                     FQVKSVPFSEIQTA"
     assembly_gap    3172..3271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3272..3625
                     /locus_tag="PLI55_07955"
     CDS             <3272..3625
                     /locus_tag="PLI55_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPB00808.1"
                     /translation="EFRRLESQALAKILESRNQVIAGGGGIILSEINRALICQKALSI
                     WLFARPLQIWPRIEQAGRPLLKSDDPLATAEQIFRTRSDLYFNTADGILDTEIATPET
                     CTDLLYAEISQIFHD"
     gene            3594..4844
                     /gene="aroA"
                     /locus_tag="PLI55_07960"
     CDS             3594..4844
                     /gene="aroA"
                     /locus_tag="PLI55_07960"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPB00809.1"
                     /translation="MQKSVKFSTIDGTVAAPASKSIVQRVIAMALLAEGTSRFGNVTL
                     CDDSQAAIEIARALGAEIELTERNLTLKGGFKPRKKVLDCGESGLCSRLFAAVAALSP
                     QELILTGRPALRKRPADMIPEPLQKLGARCESDNGHLPLKISGPLKGGNVKIDGSVSS
                     QFLTGLLIALPVVAADSEISVDNLKSKPYIDLTIEILEKFGIHIENYNYCRFIIPGGQ
                     KYQATEFNVEGDFSGAAFLLTAGALRGRITVTGLNPKSQQADRAILDLLQQCGTRVKI
                     SPDEISVTQSELQAFTCDANHYPDLFPPLVALAAGCEGVSQIAGVERLLYKESDRAAV
                     LKTEFGKLGIDINIEGDAMLIKGGKIQGGLTEAHNDHRIAMALAIAGLCSEKPVVIAG
                     AECVTKSYPTFFDDLKSVGGNIDE"
     gene            4837..>4970
                     /locus_tag="PLI55_07965"
     CDS             4837..>4970
                     /locus_tag="PLI55_07965"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPB00810.1"
                     /translation="MNSFGRLFRLTIFGESHGPAVGVIIDGCPPGLEIKESDLTADLD
                     R"
     assembly_gap    4971..5070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5071..5576
                     /locus_tag="PLI55_07970"
     CDS             <5071..5576
                     /locus_tag="PLI55_07970"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPB00811.1"
                     /translation="QARRDGDSLGGIIECRAQNLPVGLGEPFFDSVESVLAHAIFAIP
                     GIKGIEFGSGFAAARMRGSEFNDCFIAPSGKTATNHSGGINGGITNGNELVFRIVARP
                     TASIARSQQTLDFDSGKMTDLSISGRHDVCFALRLPVIVETLTACCLTDLLLIQKSWQ
                     LNEENNL"
     gene            5573..6628
                     /locus_tag="PLI55_07975"
     CDS             5573..6628
                     /locus_tag="PLI55_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HPB00812.1"
                     /translation="MNLEQIRSQIDLIDGKIIKLLNDRLSQAIMAKKFKTNIEDPQRE
                     QEVFNNLELKTYDLVTPEFARRLYAIIIEEGKRLQSQDLPVIAFQGEHGAYSEMAGKA
                     WNPQLVSIPCREFAELFEGVSDGSYDYGIIPIENTLGGVVGPVNELLISTKLNVVGAI
                     EMPIRHCLLAVRNSDYRELRSAYSHPQALAQCKHFLERNKLDPIPYYDTAGAARMVAE
                     KGLKGIAAIASPLAAQLYNLEIIKEQIEDSPLNTTRFLILAHEPFTGKGEKCSIIFST
                     AHKAGTLFKVLEIFAKAKINLTRIESVPKSPGQYAFFLDFIGSKDDPKVEKVLEEVAQ
                     STSDFRLMGCYLERILA"
     gene            6625..7410
                     /locus_tag="PLI55_07980"
     CDS             6625..7410
                     /locus_tag="PLI55_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007174341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPB00813.1"
                     /translation="MKIFILGTGRMGAWLVEEFCHQHQVAVYDRDLNKLKCFDNVERF
                     SDLNEVTSFQPELAINAVSPQNTVSAFEELLPLIPANCMLSDIASVKAGVAEFYRKIG
                     RRFVSSHPMFGPTFANIRDLRNENAIIISESDPAGKAFFRHFFENLQLNIFEYTFEEH
                     DQTTAYSLGTPFVSTLTFAANLKMQKVPGTTFRKHLEIARGLLSEDDYLLSEVLFNPY
                     TLQQIELINSRLSFLTHIIRGRDYEEMKRFLDNLRKNISALKE"
     gene            complement(7539..8192)
                     /locus_tag="PLI55_07985"
     CDS             complement(7539..8192)
                     /locus_tag="PLI55_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00814.1"
                     /translation="MIQLDPIKHFDRHLNTTELALMNSLDSPLKIQTFLDETPYSTEP
                     IYRSPLSVLRDHRAHCFDGALFAAAALRRLGYPPLIVEMLPNERDDDHLLAVYKRSNH
                     WGAVAKSNFVGLRYREPIFRTLRELILSYFEDYFNIAGEKTLRGYTLPLNLTICDRWN
                     WMIDDAHLDEIANCLDRQRRVNLLTNEMEQNLAPVDQRMLKAGLLGSNPGGLYKPDK"
     gene            complement(8385..8804)
                     /gene="rnk"
                     /locus_tag="PLI55_07990"
     CDS             complement(8385..8804)
                     /gene="rnk"
                     /locus_tag="PLI55_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPB00815.1"
                     /translation="MTKEREIYITSQDLKRLKKELSMAGISYFHGRGDLQLLAKELDR
                     AKIIEASEIPDKIVTMNTRLIFRDLDDDSTMEVTIVFPIDANINKGKISVFSPIGTAL
                     LGYSEGDTIEWKVPAGITRIRIEKVLYQPEASGEFNL"
     gene            complement(8833..>9724)
                     /locus_tag="PLI55_07995"
     CDS             complement(8833..>9724)
                     /locus_tag="PLI55_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPB00816.1"
                     /translation="HFYISLIVIDMRIISKNGTVNLLYQTLLLTSGSILCALAVKALI
                     LPHGLLARGMTGFALIIYYIWPVIPLGLLYCLINIPVFLLGWRFVGRRFIKYSLLGMV
                     IYSATLSLINFELNLSDPLLAVIIAGALSGAGTALILRSYGSSGGSDILCVILNKLFS
                     ITLGTGAILINAVVLILSAFIFPIEKVFYTLVFIFVSGLFTNKIFHGLANRRTAIIIS
                     DQWEIIVEKLNAHRIGVTLIKGQGGYRGAGCTLLYSVIPARSVSLLKRLVTEVDAQAF
                     IAIMEADDVTGVEIGNQPHW"
BASE COUNT         2639 a         2192 c         2156 g         2537 t
ORIGIN      
        1 ttttttagtg aaagactgat atgaataaca aaagatactt tttttactat ttttacttta
       61 aggaaacaca ccggaagggg taaccttact tttaaatcaa tttattaaaa accccttccg
      121 ttgagaaggg gtttttgtta caggcagact tgaaaaggag aaataaatgg gtaattctga
      181 aaatctaaaa ttgacttcca tcgaaagtca tttgaataag actactatca agatcgggga
      241 tgtaaccatc ggacaggatt tcgtcgttat cgccggacct tgcgccgtcg aatcggaaga
      301 gcaattaatg caaaccgctg aggcggttaa agccgccgga gctaatatgt tgcgcggtgg
      361 cgctttcaaa ccgcgtactt cgccttacga tttccaaggg ctcggtttaa aggctttaaa
      421 attattagaa aaagctaaaa agaaatatgg attgccgatt gttaccgaag tgttggattc
      481 gcgcgacgtc acctggattt gcgaatatgt tgacattctc cagattggtg ctcgcaatat
      541 gcagaatttt gcgcttctga aagaagtggg taagagttcc aagccggtgt tgctgaaacg
      601 cggtatgtat tcgactttga aagaatggct aaactgtgct gagtacattc tttccgaagg
      661 taatccgaat gtgattttgt gcgaacgcgg tattcgcacc tttgaaactt acacccgaaa
      721 tacgctcgac ctgagcattg ttccggctgt ccgagaagtg actcacctac cagttatcgt
      781 tgatccgtcg catgccacgg gacgattagg actgatcaag ccgatgtctt tagccgcggt
      841 agctgccggt gtggacggat taatcatcga agttcattat aaaccggaaa ccgctcttag
      901 cgatcgcgac cagcaactga atccggaaca attcaaagtg cttatgcgcg atgtattgag
      961 tctgaaaaat ttcatgcaaa ctctaagtaa ttcgaacaat gccagccatt caaattaaaa
     1021 ctgctggcaa aacttccacg attataactt gtccttcaat tcgggaagtc ttccgattga
     1081 tcgaagggcg accggtcgta ataataaccg accaaaatct cgatcgttcc tacggcgatc
     1141 tgtttcctga attccccaaa atcgttttgt cgaacggcga gagctccaag agtcttgaga
     1201 ctgtccggca gatttatgaa cagttaggca ctgccgaagt ggaccgcgat tatacaattc
     1261 tgggaatcgg tggcggcgtg gtttgcgaca tcgccgggtt cgtagccagc acttacctgc
     1321 gcggattaga tttcggattt gtagcgactt ctctgctggc tcaagttgat gccagcatcg
     1381 gcggtaaaaa cggcgttaat tttgccggtg caaaaaacat tatcggcacg atcaatcagc
     1441 cgcgttttgt gctttgtccg ctggatgtgc tggagacctt accgcgcgcg cagtttatca
     1501 atggcacggc tgaaattatc aaaaccggtg ccatcggcga cgccgattta ttggaaatgt
     1561 tggcaaccca acaagcccaa ttcatcaacc tcgtgcctga tgtgactgag caaatcattt
     1621 tccgcacgct gaatttcaaa gccggcatcg tgatgcgtga cgaacgggaa caaggtgagc
     1681 gtcgcaaact taactttggt cacacgatcg ggcacgctat tgaaattgca tcaggcattc
     1741 tgcacggcca ggcggtagcg atcggaatga atatagccgc ggaaatctcg gtgcgattgg
     1801 gtttattggc gcgtagtgaa aaagaacgcc tggagaaaat attaaccgct tacgaattgc
     1861 cgactgctac ctcattaccg gctaatgagc tcctgccgtt aattttcaaa gataaaaaac
     1921 gtcagggcga gcatctgcgc tttgtagcct taaaaaaatt aggttcagct gaagtaatta
     1981 atctctcatt taaccaaatt aaggaatttc tcgatgatct gtgttgcgtt ggctgaactc
     2041 aatttcccgg aaataatggc cattttaaaa acagaagaaa tggtagaaat ccggattgat
     2101 ttgctcaatt tgactgaaac ccagataatc gcaattttcc gaagccaccc gaatctggtt
     2161 gcaacttgtc gcaaaggccg ctattccgac gaagagcgtc aggctttact ggcgcttgcc
     2221 attcggaacg gggcggctta cgttgatctc gaatgcgatt ctccagaaga ctggcgcaga
     2281 tcgctgaccc aattagcgcg cgaacatcat acccgcctta ttatctcgta tcataatttc
     2341 gaactgacgc cgggtttggc ggaattacat ctgcttttgc gaaattgtga ggcattacct
     2401 tctgatattg tcaagattgt aacatattgt caaacggaaa gcgatgcgga acgtttgtta
     2461 agtttatatt cgctaaacac caagccgctg attgctttcg ggatgggcga agccggtcga
     2521 aagtcgcgcc ttaaagcgct ccgactcgga gcacctttca tttacgccgc ggctgaaacc
     2581 ggtcaaccta ctgcgcccgg acaaatgacg aaaaatgaaa ttctagaact gcttgctgag
     2641 gagtcggcga atgtctgaaa aacgctttgc cattatcggc catccaattc tgcacagcaa
     2701 aagcccccag attcataatc actggtttcg tgaaaataat ttgtccgcca attatagccg
     2761 gataatcgcc aatgacgtat atgttgcgct cggaatggga tacaagctcg gtcttaccgg
     2821 cgccaacgtc accgcgccgt tcaaggaaaa agtcataccg tcattagttt acctctcgcc
     2881 agaagccgcc gcggttggag ccgtcaatac tctgatctat aatggtccta atattagcgg
     2941 atataatact gatgttaccg gggttattcg tgcttttcaa attaataatg ttgaattaaa
     3001 gcacaagtct ctgctcgtaa tcggggcggg tggtgccgcg cgggcgatcg tcagcgccct
     3061 caaaccttcc ggcgccgaga ttaccattgt gaaccgtccg gatgaaatgg ctttgaatct
     3121 ggcgcgcagt tttcaagtta aatctgtgcc cttcagtgaa attcaaactg cnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaatttcgc cggttagaat ctcaagcgct
     3301 ggcaaaaatt ttagaatccc gaaatcaagt catcgccgga ggtggaggaa tcattttaag
     3361 cgagataaat cgggcgttga tttgccaaaa agcactgagt atctggctct ttgcccgacc
     3421 gctccaaatc tggccacgaa ttgaacaagc cggtcgtcca ctactaaaaa gcgatgaccc
     3481 attagctact gctgaacaaa ttttcaggac gcgctccgat ttatatttca acacggctga
     3541 cggcatttta gacactgaaa ttgccacgcc ggaaacctgt acggatttac tctatgcaga
     3601 aatcagtcaa attttccacg attgacggta ccgtcgccgc tccggcttct aagagcatcg
     3661 ttcagcgcgt tattgcgatg gctttgctgg cggaaggtac ttcccgcttc ggcaatgtta
     3721 cgctttgcga tgacagtcag gcggctatcg aaatcgcccg agcgctcggt gcagaaatag
     3781 aattgactga gcgtaatctt accctaaaag gtggtttcaa gccacgtaaa aaagtactgg
     3841 attgcggcga atcgggattg tgttcacgct tgtttgcggc ggtagcggcg ctcagcccac
     3901 aggagttgat tctcactggt cgtccggcac ttcgcaaacg tccggcagat atgattcccg
     3961 aaccgcttca gaaactcgga gcccgatgcg aatccgacaa tggtcattta ccattaaaga
     4021 tcagcggtcc gctaaaaggg ggcaatgtaa agattgacgg ttccgtaagt tcgcagtttt
     4081 taaccggttt actgatcgct ttgccggttg tcgccgccga ttctgaaatc agcgttgata
     4141 atctaaaaag caaaccatat attgacttaa ccattgaaat tttggaaaaa tttggtatcc
     4201 acatcgaaaa ttataattac tgccgtttca ttattccggg cggtcagaaa tatcaagcga
     4261 ccgaattcaa tgtcgaaggt gacttcagtg gtgcggcttt tttattgacc gctggcgctt
     4321 tgcgggggcg gattaccgtg acaggactta atcccaagtc ccagcaggct gatcgagcaa
     4381 ttttggattt actacaacaa tgcggtacgc gggtaaaaat cagcccggac gagattagcg
     4441 tcactcaatc cgaattgcaa gcgttcacct gcgatgcaaa ccattatccg gatctttttc
     4501 caccgttggt ggctctggcg gcagggtgtg aaggtgtcag ccaaatcgcg ggcgttgaac
     4561 gcttacttta caaagaaagt gatcgagcgg cggttttgaa gacggagttc gggaagcttg
     4621 gaattgatat taatatagaa ggcgacgcga tgttaatcaa aggtggtaaa attcaaggtg
     4681 gtttgacaga agcccataac gatcaccgta ttgctatggc gctggcgatt gccgggctat
     4741 gttcggaaaa gccggttgtg atcgcgggcg ccgaatgtgt taccaagtcc tacccgacat
     4801 tttttgatga tttaaaatca gttggaggta atatcgatga atagttttgg acgactcttc
     4861 cgcctaacga ttttcggcga gtcacacgga ccggcggtcg gggtaataat tgacggttgc
     4921 cctccgggat tggaaattaa agaaagtgat ttaactgctg atctcgatcg nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcaagcccg ccgcgacggc gactcgctgg
     5101 gcgggatcat cgaatgccgc gctcaaaatt tgccggtcgg gctaggcgaa ccctttttcg
     5161 attcggtaga atcggttctg gcgcatgcca tctttgccat tcccggtatc aaaggcatcg
     5221 agttcggcag tggatttgct gccgctcgta tgcgcggtag tgaattcaat gactgcttca
     5281 tcgcaccgag cggcaagacc gctacgaatc attctggcgg aataaatggt ggaattacca
     5341 atggcaacga gctcgttttc cgcatcgtag ctcgaccgac tgccagcatt gcccgcagtc
     5401 agcaaacttt agattttgat agcggaaaaa tgacagattt atccatcagc ggtaggcacg
     5461 atgtctgctt tgctttacgc ctgccggtga tagttgagac tctgacggct tgctgtctaa
     5521 ccgacttgtt attaattcaa aaatcatggc aattaaatga ggaaaataac ttatgaacct
     5581 ggaacaaatt cggtcacaga ttgacctgat cgacggcaaa ataatcaaat tgctcaatga
     5641 ccgtctgtca caggcaatta tggccaaaaa attcaaaacc aatatcgaag accctcagcg
     5701 cgagcaagag gttttcaata atctggaact aaaaacttac gacttggtca ctccggagtt
     5761 cgctcgacgt ttatacgcca ttatcatcga agaaggcaaa cgcttgcaat ctcaagacct
     5821 accggtaatc gctttccagg gcgaacacgg cgcctatagc gaaatggctg gcaaagcctg
     5881 gaatccgcaa ttagtcagca ttccttgccg cgaatttgcc gaactattcg aaggcgttag
     5941 cgacggctcc tacgattacg ggattattcc cattgaaaat actctcggcg gagtagttgg
     6001 accagtcaat gaattgctca tttccactaa attgaatgtc gtgggtgcaa tagaaatgcc
     6061 gatacgtcat tgtttactgg cggtgcgcaa tagcgattac cgcgaactgc gcagtgccta
     6121 ttcccatccg caagctttag cccagtgtaa acatttttta gaacgtaata aactcgaccc
     6181 gataccctac tacgacacgg cgggcgcggc tcgtatggtt gctgaaaaag gtttgaaagg
     6241 aattgccgca atcgccagtc cgctagccgc tcaactttac aatctggaaa ttatcaaaga
     6301 gcagatcgaa gattcacctt tgaacactac tcgctttttg attctggctc atgaaccgtt
     6361 caccggaaaa ggcgaaaaat gttcaatcat tttctcgacc gctcataaag cgggcacgct
     6421 cttcaaagtg ctcgaaattt ttgccaaggc gaaaatcaac ctgacccgca tcgaatcagt
     6481 ccccaaatcg cccggtcaat atgccttctt cctcgatttt atcggctcta aagatgatcc
     6541 caaagtcgaa aaagttttag aagaagtcgc acaatctacc tctgatttcc gcttgatggg
     6601 atgctattta gaaaggattc tggcgtgaaa atatttattc tgggaacggg ccgtatgggt
     6661 gcctggctgg tggaagaatt ttgtcatcaa caccaggttg cagtttacga tcgcgattta
     6721 aataaattga aatgtttcga caatgttgaa cgtttttccg atttaaatga ggtgacgagc
     6781 tttcaaccgg agctggctat caatgccgtt agcccgcaaa ataccgtatc ggctttcgag
     6841 gaactcctgc cgctcattcc tgctaattgt atgctgtctg atattgcctc ggttaaagcc
     6901 ggtgtggctg aattttaccg taaaatcggc cggcgcttcg tttcatctca tccgatgttc
     6961 ggacctactt ttgccaatat tcgcgatctg cgcaacgaaa acgctattat tatcagtgaa
     7021 tctgatccgg caggtaaggc ttttttccgt cattttttcg agaacttgca actgaatatt
     7081 ttcgaataca ccttcgaaga acacgatcag accacggctt attctttagg cacacccttt
     7141 gtttcgacgt tgaccttcgc cgccaatttg aaaatgcaga aagtgcccgg tactaccttc
     7201 cgcaaacatc ttgaaatcgc ccgcggacta ctttcggagg acgattatct actatcggag
     7261 gtactcttta acccctacac gctccagcaa attgaactta tcaattctcg tctgtcattt
     7321 ctaacccaca tcattcgggg acgcgattac gaggaaatga agcgattctt agacaatttg
     7381 cggaaaaaca tcagcgcact aaaagaataa ccagcattat ttttctgata ctatttccaa
     7441 tgctcgcgtt aggttatgcc attcagctat tcttatagtt gttcggatta atcccaaaat
     7501 taggaacgaa agtaatttct atcattaggg tcaataattt atttatccgg tttatacagt
     7561 cctcccggat tcgatcccag cagaccggct tttagcatac gctgatcaac tggtgcgaga
     7621 ttttgttcca tctcatttgt gagcaaattt acgcgacgct gacgatcgag acagttagcg
     7681 atttcatcca gatgtgcatc gtctatcatc caattccagc ggtcgcaaat agtaagattc
     7741 agcggcaaag tgtagccgcg caaagttttc tcgccggcga tgttgaaata atcctcgaaa
     7801 tatgaaagta ttagctcccg cagagtgcgg aaaatcggct cacggtagcg cagaccgacg
     7861 aagttggatt tagctactgc accccagtga ttactgcgtt tgtaaaccgc cagcaaatga
     7921 tcgtcgtcgc gctcgttggg taacatttct acaatcaatg gtggataccc gagtcgccgc
     7981 aaagccgccg ccgcaaacaa agctccgtca aagcaatgtg cccgatgatc acgtagaaca
     8041 cttagcggtg atcggtaaat aggctcggtg ctgtagggcg tttcatcgag aaaagtctgg
     8101 attttcagcg gcgaatctaa actattcatt aaagcaagtt ccgtcgtatt taaatgacga
     8161 tcaaaatgtt ttattgggtc tagttgaatc atttttcgat ctttccaatt tagtctgttt
     8221 atcaatgatg agttcgattt tgcaaaaata tttatcctat gaatgggatt ctaaaagaat
     8281 aataggcatt ctcaattttc actatcttct tatcagcagc cctgaaaagt cgtagatgta
     8341 gctccctacg ttcctggcac tcaccaagtt aaattcgcct tttgttatag attgaactca
     8401 cccgatgctt ccggctggta taaaactttt tcgattcgaa ttcgggtaat tccagctgga
     8461 accttccact caatagtatc tccctcagaa taacctaaaa gtgccgtccc gatcggcgaa
     8521 aaaaccgaaa ttttgccttt attaatgtta gcgtcaatgg gaaagacgat ggtaacttcc
     8581 attgtgctgt catcatccag atcacggaaa attagacgcg tattcatagt gacgattttg
     8641 tctgggattt cgctagcttc aataatcttg gctctgtcaa gctccttcgc caatagttgc
     8701 aaatcgcccc gtccatggaa atatgaaatc ccagccatag aaagttcctt ttttagacgt
     8761 tttaaatctt gagaagtaat ataaatctcc ctttccttcg tcatattgca cctttctcta
     8821 ttttttgctt tattaccaat gcggttgatt gccgatttcc actccggtga catcatcggc
     8881 ttccataatg gcaataaatg cctgggcatc tacttccgtt accagacgtt ttaataatga
     8941 aactgaacgc gccggaatca ccgaatataa caatgtacac ccagccccgc gatatccacc
     9001 ctgtccttta atcaaagtca ctccaattcg atgtgcgttt aatttttcga cgataatttc
     9061 ccattgatcg gagataatta tggcggttct acggtttgcc aagccgtgaa aaattttatt
     9121 cgtaaacaga ccactaacga aaatgaaaac aagagtgtaa aaaactttct ctattggaaa
     9181 aataaatgct gacaaaatca acaccaccgc attaataaga atggcaccgg tgcccagggt
     9241 tattgaaaat aatttgttca atattacgca aaggatgtct gaacccccac tggaaccata
     9301 agaacgtaaa attagcgcag tccccgcacc ggataaagca ccggcaataa taacggctaa
     9361 aagcggatca gatagattaa gctcgaaatt aattaacgaa agagtcgcag aataaatcac
     9421 cattcccaac agagagtatt tgataaaacg gcgacccaca aaacgccaac cgagcaaaaa
     9481 aaccgggata ttaataagac aataaagcaa tcccagcgga atcaccggcc atatatagta
     9541 aatgattaaa gcaaaacccg tcattccacg ggcaagcaga ccatgcggta ggattaaagc
     9601 tttcaccgct agagcacaaa gaatactacc agacgtgagt aataaggtct gatataataa
     9661 attaacagtt ccgttttttg aaatgattct catatctatt acaattaaac taatataaaa
     9721 gtgt
//
LOCUS       DAOOMU010000082         9650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_8209_length_9650_cov_21.876290, whole genome shotgun sequence.
ACCESSION   DAOOMU010000082 DAOOMU010000000
VERSION     DAOOMU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8209_length_9650_cov_21.876290"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..306)
                     /locus_tag="PLI55_08000"
     CDS             complement(148..306)
                     /locus_tag="PLI55_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00817.1"
                     /translation="MDNYESITTALKIPFFPLGLRSPARDVLNAVMIVKTINFSRPDC
                     LFTQSLYQ"
     gene            complement(655..1875)
                     /locus_tag="PLI55_08005"
     CDS             complement(655..1875)
                     /locus_tag="PLI55_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB00818.1"
                     /translation="MKRIKIIGLFMLMATTICAADELTLEQCIEAAVANNKSLVKNEL
                     GVQQTAIGTKTAYGTLLPSVSASASQSGGDGSSRSSASLQINQKIYSPGLFSDIKLAR
                     NETDIARLNYNELASQIRSEVETAYYNILTAQSLLNVYQENLTAADENLQLIQTMYKL
                     GTKTESDVLKAEVQKAEIENELIGQELEIINEKRSLAILMGISPDYDFEIVEIDVEQI
                     EIPPLAEAKELLFKNNPEYQSLLKSLKSQQISLQIARESYLPSLSAGYSYSKDWTGST
                     TTDGYGSWGLSLNLGLFNGFSKKLNVQKSKLSLRSAEVSLEAKEQELLATLLNYYSNF
                     ENHNKVIALQEKNLESARKDYELVTRQCELGLATMLEQTTAQVSLLSAQTNLVKAQYS
                     RKIVESQIKQLLAL"
     gene            complement(1891..>2546)
                     /locus_tag="PLI55_08010"
     CDS             complement(1891..>2546)
                     /locus_tag="PLI55_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPB00819.1"
                     /translation="LKEKGGSGPGGDQDDIILAPSTTVLYRLKGSQWIDMINASAISE
                     EAMTAAQEEIRTILREAHRLGPADGDDFNIRTQSEIIDFASSTSKTMTLLLGAVACVS
                     LVVGGIGIMNIMLVSVTERTREIGIRMSVGARASDILSQFLSEAVVLSLSGGIIGIIL
                     ALAASALLNNLTDLRTVIDPVIVILAFTFSGAVGIFFGWYPARKAANLNPIDALRYE"
     assembly_gap    2547..2646
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2647..3266)
                     /locus_tag="PLI55_08015"
     CDS             complement(<2647..3266)
                     /locus_tag="PLI55_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB00820.1"
                     /translation="MKKIYNLLKVAIKSIFKNRMRSLLTSLGIIIGVSAVIIMVAVGR
                     GSQAAIEDNIKSLGTNLIIIFPGASRSGGASMGAGSFNRFTMKDVERIKESATLISAI
                     SPVVRANGQVIYKSNNWYTSISGVSPDYFSITNWELESGEFFTERDDLARKKVALLGK
                     TVATELFGADNPVGEQIRIRNVPFTVIGVLKEKGGSGPGGDQDDII"
     gene            complement(3263..4018)
                     /locus_tag="PLI55_08020"
     CDS             complement(3263..4018)
                     /locus_tag="PLI55_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB00821.1"
                     /translation="MPTVIEVRQLSKTYQVGDIEVHALRKVDFAVAAGEFTAIMGASG
                     SGKSTLMNMLGCLDQPTGGEYLLDGVNVSTLTSDERADIRNQKIGFVFQGFNLLPRTT
                     ALENVELPLIYDRHQRIKNPQSAAKEALERVGLGDRMHHIPTQLSGGQQQRVAIARAL
                     VNQPSILLADEPTGNLDSKTSMEIFSLFQQLNDEGLTIVMVTHERDFATYAKRILQMR
                     DGVLHKDTLIKKRRNAAEDLKMLIAKQQQEEEQ"
     gene            complement(4063..5340)
                     /locus_tag="PLI55_08025"
     CDS             complement(4063..5340)
                     /locus_tag="PLI55_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB00822.1"
                     /translation="MKKKICLIVILVLAVAVGWHLIRKNGSDKQIQIAYGQVTRGNIE
                     TIISSTGTLVAIGTVDVGTQVSGRIDKVLVDYNDIVKKDQVLAVLDTLLLSQSVKEAE
                     ANLHKMQALYEQAAFQYDRISNLFQQKLVSEQEYIDARTNKATSEVSLKQAQIALDKA
                     VADYGYAIIRSPISGTVIDRVVEEGQTVAASFSTPTLFTIARDMTKMEIEALVDESDI
                     GEIKTGQKVRFTVQSYQDKIFNGVVRQVRLQPQTVSNVVNYTVIVDADNHEQLLLPGM
                     TATVDFIIDSRENVLIVPNSALKITPTEEMLAKAHQVRQAQMEQLPESVKTKLREQSP
                     KPQLADLSPEELDKSTRPTPIWYVNESGQILMALVKTGLSDTKNTEILENPEIKEGTK
                     IIVSLIANGKNSVSNSSNNQQRGMGGPGGPPPF"
     gene            5490..6053
                     /locus_tag="PLI55_08030"
     CDS             5490..6053
                     /locus_tag="PLI55_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPB00823.1"
                     /translation="MENLASLTDQEIIDACLSGNENAFAEIIRRYEPRVAATVIGMLG
                     RCPEAEDVGQETFIRFYKSMSKFRGESSLSTYLTRIAMNLSINELNRRKRQSRFVPLD
                     DPEISRKTSGDSGNQADSESGAIIRRAVAQLAPKFRSTIVLRLIEGYSTEETAQILQL
                     PVGTVLSRLARAKVQLMNLLTPYKELL"
     gene            6050..6478
                     /locus_tag="PLI55_08035"
     CDS             6050..6478
                     /locus_tag="PLI55_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00824.1"
                     /translation="MKIDKIRKLLLLSGDRNLTAKEQKILMSARQTNPELAEDEQLLQ
                     KIQQNLAGTKQESFEPWFAERTMNRIRQLASQEKHPARLWNGFWPAYSRVIIGAAVIL
                     ALIVVFNLFTSSEPSLAKAFGITQTPIEAIMDPIAYLIWE"
     gene            6484..6897
                     /locus_tag="PLI55_08040"
     CDS             6484..6897
                     /locus_tag="PLI55_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00825.1"
                     /translation="MKKTTRTTLMLSSVLILGIIIGFVGASSWLHYNQKKRFTHFRKG
                     EGFVVEMEKMIDPLPEQKDKIHEILVRHSLWVKQFSEEQRAILQRSIDSLDAELARVL
                     TPEQMKRFQEKVKNQPRRPGPPPGPPPPPFEEPRP"
     gene            7451..8308
                     /locus_tag="PLI55_08045"
     CDS             7451..8308
                     /locus_tag="PLI55_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00826.1"
                     /translation="MRKISKKTLLEMGVLMALLPSCKKATVQSAWNDGSIIIDAVVKD
                     WGSRLVWNEKSQVSLGVTNDAENLYLCLVTSDPDIHRQILINGFIVGIDQKGSKSHHF
                     GIKFPLGIGDRGNQLLTPTDDQHRQKPFEPGQPGQKGRENDFLAKFNEQQTELILLGP
                     GDKQKTLVPLENDLGLEVKIGFDRQQLVYELKLPFSVISQHLQIDQISNNKPLTVSFK
                     TDKVEIPSGRPGDMMDSPGGMGRPEGGMGGPGGRMGVPDARMGNPGGDEHSEKTFSYQ
                     VAVTLAVKP"
     gene            complement(8519..9472)
                     /locus_tag="PLI55_08050"
     CDS             complement(8519..9472)
                     /locus_tag="PLI55_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPB00827.1"
                     /translation="MANKTIELKGVDPALVFGVRDVFINKIEESFGARIFARGTKINL
                     EGDENTLSQIERVIGQMLMTINRKGFVDEQDVTTLINMELGGQQLPGEITADTVILFK
                     KTGVIKPRSVNQEHYFSATLKNDLVFGIGPAGTGKTYLAVAIAVAALRNHDVQRLILT
                     RPAVEAGESLGFLPGDLTEKIEPYLAPLYDALYDMLPPEKVRVYEEQHIIEVLPLAYM
                     RGRTLNNAYVILDEAQNTTPMQMKMFLTRLGANSKAIVTGDITQIDLPDRQKSGLIDA
                     LEILRGIEGIEFVYFDTTDVVRHRLVKKIIKAYNGESPNKS"
BASE COUNT         2617 a         2119 c         2106 g         2708 t
ORIGIN      
        1 gaacttgata acttttgcgc cttaaattcc attcgctggg actgagcgaa cccccgtgag
       61 atgttcttta gccgttgcct tttttggttc ttttttcgtc aaaggaaaaa agaacaggaa
      121 ggttgggcat tggtcattgg tcattagtca ttggtaaaga gactgagtaa agagacagtc
      181 tggacgacta aaattaatgg ttttaactat cattacggca ttaagaacat ctcgggctgg
      241 ggagcgaagt cccagcggaa aaaatggaat tttcagcgct gtagtaatac tttcgtaatt
      301 atccattacc ctttcctatg tcacttacct tatatgagct tgataacttt tgcgccttaa
      361 attccattcg ctgggactga gtgaaccccc gtgagaggtt ctttagccgt tgcctttttt
      421 ggttcttttt tcggcaaagg aaaaaagaac agccaaactt gttttaacaa aaataacaaa
      481 gacacgaaaa atagggctga gaaagaactt aaatcttagc cttttttctc agccttagtc
      541 tttcttttag gctgaggtag agaagaatgg aaaatgtcgc cctgatctca tcgaagggcg
      601 aactcattga tgaatgatga cttatagcaa cgaatgattt gccccgccgc gctatcataa
      661 tgccagcagt tgtttaattt gggattcgac aattttacga gaatattggg ctttgacaag
      721 attggtttga gctgaaagaa gcgagacctg agcggttgtc tgttccaaca tagttgccag
      781 accgagctcg cattggcgcg taacaagttc gtaatctttg cgggctgatt ccagattctt
      841 ttcctgcaga gcaataactt tattatgatt ttcaaaattg gaatagtaat tcagcaaagt
      901 ggcaagtaat tcctgctcct tagcctctaa actgacttcg gcgcttctaa ggctgagttt
      961 gcttttctgg acatttagct ttttactgaa tccgttgaat agcccgagat ttaatgacaa
     1021 accccacgaa ccgtagccat cggtcgtagt ggaaccggtc caatctttcg aatagctata
     1081 accagcgcta agcgagggca gatatgactc tcgggcaatt tgcaggctga tttgctggct
     1141 ttttaacgat tttaacaaag actgatattc tgggttattt ttaaacaata gttccttagc
     1201 ctcggctaaa ggcgggattt caatttgttc aacgtcaatc tcgacaatct caaaatcata
     1261 gtcgggagat attcccatca gaatcgccag tgagcgtttt tcattaataa tttcgagttc
     1321 ctggccaatc aattcatttt cgatttccgc tttctgcact tcggctttca atacatcaga
     1381 ttcggtcttc gtgccgagtt tatacatcgt ctgaattaat tgcagatttt catcagccgc
     1441 cgtcaggttt tcctgataga cattcaacaa actttgcgca gttaaaatgt tataataggc
     1501 ggtttcgact tcactccgaa tctgggaagc taactcgttg taattaagac gagcgatatc
     1561 agtctcattg cgggctaatt ttatatcgga aaataatcca ggcgaataga ttttctggtt
     1621 aatttgcaaa ctagcgctcg atcgcgaact gccatctccg ccgctttggc tagcgctggc
     1681 actcaccgat ggcagtagag ttccataggc ggttttggtg ccaattgcag tttgctgaac
     1741 gccgagttca ttctttacta gcgatttgtt attagcaact gccgcttcga tacattgttc
     1801 gagagtaagc tcatctgcgg cgcaaatggt cgtagccatt aacataaaca agccaattat
     1861 tttaattctt ttcataagaa caaatccttt ctattcataa cgcagggcat caatcggatt
     1921 gagattagcg gctttgcgcg ccggatacca gccgaagaaa ataccaactg cacccgaaaa
     1981 agtgaatgcc agtattacga ttaccggatc aatcacggtt cgcaaatcgg tcaaattatt
     2041 taaaagggct gaagccgcca aagccagaat aatgccgatg atgccacccg atagactaag
     2101 aacaacggct tccgatagaa actgactgag aatgtcgctg gcgcgggctc caaccgacat
     2161 acggatgccg atttcgcgcg ttctctcagt gactgatacc agcataatgt tcattattcc
     2221 aatgccaccg actaccagcg atacgcaagc cacggcaccc aggagcaagg tcatagtttt
     2281 ggaagtcgaa gaagcaaaat caatgatttc cgattgagtg cgaatattaa aatcatcgcc
     2341 atcagccggg ccaaggcgat gagcttcacg cagaatggta cggatttcct cttgagcggc
     2401 ggtcattgct tcttcgctga ttgcgctggc gttaatcata tcaatccact gtgaaccttt
     2461 taaccggtat agcacagttg tcgaaggtgc taaaataatg tcatcttggt cacctcccgg
     2521 tcccgagccg cctttttcct taagaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnaaaa taatgtcatc ttggtcacct cccggtcccg agccgccttt ttccttaaga
     2701 acgccgataa cggtaaaagg aacattgcga atgcggattt gctcacccac cggattatcc
     2761 gcgccaaata gctccgtagc caccgttttc cctaacaggg caactttttt gcgggctaaa
     2821 tcatcgcgtt ccgtaaaaaa ctctccactt tccagttccc agttggttat cgaaaaatag
     2881 tccggtgaaa cgccactgat actcgtgtac caattattgc ttttataaat cacctgaccg
     2941 ttagcacgga cgaccggcga aatagcgctg attaatgtag ctgattcctt aattctttct
     3001 acatctttca tcgtaaaacg gttaaaactc ccagcgccca tactggcacc gccgctacgc
     3061 gaggctccgg ggaagatgat tatcagattg gtgccgagcg actttatatt atcctcgata
     3121 gcggcctgcg aaccgcggcc gaccgccacc ataatgatga cggcgctgac gccgatgatt
     3181 attccgagcg aggttagcag actgcgcatc cggtttttaa atatgctttt gatagcaact
     3241 ttcaagaggt tataaatttt tttcattgct cctcctcttg ttgctgtttg gcgattagca
     3301 ttttaaggtc ttccgccgca ttacgccgtt ttttaattag ggtatcctta tggagaactc
     3361 catcacgcat ttgaagaata cgtttggcat aagtggcaaa atcgcgttcg tgagtaacca
     3421 taacaattgt taaaccttca tcatttaatt gctggaagag actaaaaatc tccatagacg
     3481 ttttgctgtc cagattgccg gtaggttcgt cagccagtag aatcgaaggc tggtttacca
     3541 gcgctcgagc aatggcgacc cgctgttgct gaccgccgga aagctgggta ggaatatggt
     3601 gcatacggtc cccaaggcca acgcgttcca gcgcttcttt ggcggcgctt tgagggttct
     3661 ttatacgttg atggcgatca taaatcaagg gcagttcgac attttccaga gcggtggtgc
     3721 gtggtagaag attgaatccc tggaagacga atccgatttt ttggttgcgg atatcggcgc
     3781 gctcatcaga cgtcagcgta ctaacgttga caccatccaa taggtattcc ccgccggtag
     3841 gttgatccaa acaacccagc atattcatta gggttgattt tccggagccg gaagcaccca
     3901 ttatggcagt aaattcaccc gccgccactg cgaaatctac cttgcgcaag gcatgcactt
     3961 ctatatcacc gacctggtag gttttactca attgccggac ttcaattacg gttggcatat
     4021 tattctctca gttctgtgtg ttttatcggt tgatctttgt gattaaaaag gaggcggacc
     4081 gccaggacca cccataccgc gttgctgatt attggaagaa ttgctgaccg aatttttgcc
     4141 gttggcaatt aaactaacaa taattttagt tccttcttta atttcaggat tttccaaaat
     4201 ctcggtattc ttggtatcgc tcaaaccggt tttcaccaaa gccataagaa tctggccgga
     4261 ttcgttgaca taccagattg gcgtcggtcg cgttgattta tctaattctt ccggcgatag
     4321 atcagccaat tgtggtttag gagattgttc tcggagtttg gtttttactg actcaggcaa
     4381 ttgttccatt tgtgcttgtc gcacctgatg tgctttcgcc agcatttctt ccgtcggagt
     4441 aatttttaac gctgaatttg gcacgatcag cacgttttcg cgtgaatcaa tgataaaatc
     4501 cacggtagcg gtcattcccg gtaataacag ttgttcgtga ttatcggcat caacaatgac
     4561 cgtataattg acgacattgg agacggtttg cggctgaaga cgaacctgtc ttacgactcc
     4621 attgaaaatt ttatcctgat aactctggac ggtaaagcgc actttctggc cggttttgat
     4681 ttcaccgata tcactttcgt ccactagggc ttcaatttcc attttggtca tatctcgggc
     4741 aatagtgaaa agagtaggcg tcgaaaaact agccgcgacc gtctgtccct cttcaaccac
     4801 tcggtcaata accgttccgg aaattggtga gcggatgatg gcataaccat aatcggcgac
     4861 cgccttatcg agggcaatct gggcttgttt taagctgact tcgctggtgg ctttattggt
     4921 tcgggcatca atgtattctt gctccgaaac caatttttgc tggaaaagat tagaaatgcg
     4981 atcatactga aaagcggctt gttcataaag cgcttgcatt ttgtgcaaat tggcttctgc
     5041 ctctttaacg gattgactca atagcagtgt atccaaaacc gccagaactt gatctttctt
     5101 gacaatgtca ttgtaatcca ccagaacttt atcaatccgg cccgaaacct gggtaccgac
     5161 gtccaccgta ccgatcgcta ccagcgtacc ggtactggag atgatggttt cgatattgcc
     5221 gcgggttact tgcccatagg caatctgtat ctgtttatcc gagccgttct tccgaattag
     5281 atgccatccg acggcgaccg ccaaaaccag aatgacgatc aagcaaattt ttttcttcat
     5341 aaatctttaa atcctttcaa ttttcaaaac cgctgattag acaaaataat gagtgatttc
     5401 attccaaatc gaacaaacta ttttttatat ctgaatggat tggaataaat tcaacagcct
     5461 attggtctaa cttaatgttt tgagaaataa tggagaattt agcgtcacta accgatcagg
     5521 agataattga tgcctgtctt tcaggaaatg agaacgcatt tgccgaaatc attcgccgtt
     5581 atgaaccgcg agtggcggct accgttattg gaatgctggg aagatgtccg gaagccgagg
     5641 acgttgggca ggaaacattt atccgttttt ataaaagtat gtccaaattt cgaggagaat
     5701 cgagtttgag cacttatctg acccggatag cgatgaattt atcaattaat gaactgaacc
     5761 gccgcaagcg ccagtcacgt tttgtgcccc tggatgatcc tgaaatctcg aggaaaacat
     5821 caggcgattc cgggaatcag gcggattcag aaagcggcgc aattattcgt cgggcggttg
     5881 ctcaactggc gccgaaattt cgttcgacca ttgttttgcg tctgatcgaa ggttattcga
     5941 ctgaagaaac tgcccagatt ttgcaattgc ccgttgggac ggtgctgtcg cgtttagcac
     6001 gcgccaaagt ccaacttatg aatttattaa caccctataa ggagctatta tgaaaattga
     6061 taaaattcgc aagctattac ttctatccgg cgatcgtaac ctgactgcca aggaacaaaa
     6121 gattcttatg tcagccagac aaactaatcc cgaactggcg gaagatgaac aactattaca
     6181 gaaaattcaa caaaatcttg ctggcacaaa acaggagtct tttgagccct ggtttgctga
     6241 aaggacgatg aaccgaattc gccaactcgc cagtcaggaa aagcatccag caagattgtg
     6301 gaatggtttc tggccggctt atagccgggt tattattgga gccgcagtaa ttctggcttt
     6361 gattgtggtt tttaatctct tcactagttc cgagccgagc ctggcaaagg cattcggtat
     6421 tactcagact ccaatcgaag ccataatgga tccgatagca tatttaattt gggagtaata
     6481 aaaatgaaga aaacaaccag aacaacttta atgctaagta gcgtcctgat tttgggaatt
     6541 attataggtt ttgtgggagc ttcaagttgg ctacactata atcagaaaaa acgttttact
     6601 catttccgca aaggcgaggg cttcgtagtc gaaatggaaa aaatgattga cccactcccg
     6661 gaacaaaaag ataaaattca cgaaatattg gtacgccatt cactctgggt caaacagttt
     6721 agcgaagagc aacgcgctat tttacaacga agtatcgatt ctttggacgc tgagctggct
     6781 cgcgtgctta ccccagagca gatgaagcgc tttcaggaaa aagtaaaaaa tcagccaagg
     6841 cgtccaggac cgccacctgg gccgccgcca ccacccttcg aagagccacg tccctaaaat
     6901 ccgggtttga ctttaaagac gtatcgtctt cttgatgaat taattaccat attattacgc
     6961 ttagtgaata taaaatttcc gactgctaat gttaagtggg gtttcctgaa aaaataaaca
     7021 cgatactatg atttacattt atatccaaca ccgacctgtc gggattgaat atacatgaat
     7081 tatatgatta taaagcctca cctcctacag ctccgcggga gttaaaataa ctataggcaa
     7141 acaatatcac caaccccaca gagtttgaat ccatctaaaa gggacgaact tgtccattat
     7201 cggctctatc gtaaaacttg tctacatttt gatttcttaa gcagacctca caaaaagccg
     7261 gagcctaatt acctttcgtt tcattttaaa atggaataaa aataggattg gctttgtcta
     7321 aactgcgagt ttgagttttt tgtaatgcga aataattcac ttgaaagtaa acgattttaa
     7381 gcaagtcgtt attaggataa aacggttgca cggtaaataa acttcaaaac aattttgtgg
     7441 aaagggaaaa atgcgaaaaa tatcaaaaaa aacattacta gaaatgggcg ttttaatggc
     7501 acttctgcct agttgtaaaa aggctacagt tcagagcgcc tggaacgatg gcagtataat
     7561 aatagatgcc gttgttaagg actggggctc acgcttggtg tggaacgaaa aaagccaggt
     7621 cagtttggga gtcaccaatg atgccgaaaa tttatactta tgtttggtaa cttccgaccc
     7681 ggacatccac cgtcagatat tgatcaacgg ttttattgtc ggaattgatc agaagggtag
     7741 taaaagccat catttcggta tcaaatttcc attgggtata ggcgaccgcg gcaaccaact
     7801 actaacgccg accgatgacc aacaccgaca aaagcctttt gaacccggtc aaccggggca
     7861 gaaaggtagg gaaaatgatt ttttggctaa atttaatgaa cagcaaaccg agttaatttt
     7921 gctgggacca ggtgataaac aaaaaacgct tgttcctctg gaaaacgact tgggactgga
     7981 agtcaaaatc ggattcgatc gccagcaact tgtctatgag ctgaaattgc catttagcgt
     8041 gattagtcag catctgcaaa ttgatcaaat ttcaaataac aagcccttga cagtcagttt
     8101 caaaactgat aaagttgaga taccatccgg tcgcccaggt gatatgatgg actcccccgg
     8161 cggaatgggc agaccggaag gtggtatggg tggtcctggc ggtaggatgg gtgttcccga
     8221 cgctagaatg ggcaatcccg gcggtgatga acatagcgaa aagacatttt catatcaagt
     8281 ggctgttact ttggcagtga aaccataaga tttggtaaac ccttttgaat aataaccctc
     8341 atatctctat cactcactcg cctacactcc tccacggcgg ttcagcagga tacacggaag
     8401 actcgtatga gtcttccgtt gcctgctgat tatataacac cttttttaac tgaacaatta
     8461 ttattgtctt atcatcaaga aaggcaatta aatcccggct tttaggggat gactacagtc
     8521 aggatttatt tggactttcg ccattataag cttttataat ctttttaacc aggcggtggc
     8581 gtacgacatc ggtggtatcg aagtaaacaa attcgattcc ctcaatgccc ctcaatattt
     8641 ccagcgcatc aattagccca gatttctggc gatcgggcaa atcaatctgg gttatatcgc
     8701 cggtgacaat cgctttggaa ttggctccca agcgggttag gaacattttc atctgcattg
     8761 gcgtggtgtt ttgggcttca tccagaatta cgtaggcatt gtttagcgtg cgcccccgca
     8821 tataagccag cggaagaact tcaattatgt gttgctcttc gtaaactctg actttttccg
     8881 gcggcaacat atcataaagc gcatcgtata gcggcgcaag ataaggttca attttttcgg
     8941 ttaggtcgcc cggcagaaat ccgagacttt caccagcttc gacagctggg cgcgtcagga
     9001 ttaagcgttg aacatcgtga ttgcgcaaag ccgccacagc gatggcgacc gccagataag
     9061 tcttgccagt tccagctgga ccaatgccaa acactaagtc gtttttcaag gtggcgctga
     9121 aataatgttc ctgatttacc gagcgcggtt tgattacgcc ggttttcttg aagaggatga
     9181 cggtatcggc agtaatttcg cccggcaatt gctgtccgcc aagttccata ttgattaaag
     9241 tggtaacgtc ctgttcgtca acaaatcctt tgcgattgat tgtcattagc atttgaccga
     9301 tcacgcgctc aatttggcta agtgtatttt cgtcgccttc gagatttatt ttcgtgcccc
     9361 gcgcgaatat tcgcgccccg aagctctcct cgattttgtt gataaatacg tctcggacgc
     9421 cgaacaccag agctggatcg actcctttta attcgatggt tttatttgcc atatttctcc
     9481 atcattgctt tcattaattg ctaatttcgc aattttattg gcaaaatgaa agccatgcga
     9541 gaaaccaacc ggatgatccc gtcagtcgag ttgtaaactc gttaaagaaa ggttaagact
     9601 gagaaagaag gctaagattt gaattctttc tcaacctcaa tctatttact
//
LOCUS       DAOOMU010000083         9617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_8246_length_9617_cov_13.801088, whole genome shotgun sequence.
ACCESSION   DAOOMU010000083 DAOOMU010000000
VERSION     DAOOMU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8246_length_9617_cov_13.801088"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            530..>2651
                     /locus_tag="PLI55_08055"
     CDS             530..>2651
                     /locus_tag="PLI55_08055"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPB00828.1"
                     /translation="MKRYFVSLVVFTLAGFLAYAQELPANRSSIDSPHHSRFSEERIG
                     KSEQFNRHPYSKFLMDLDEQPYRHPCPSESRDATSTLNLNNTATTNKEPEWISYYASG
                     LLPSPDIATSIAVDKHGNVYVTGYSVEALFGADYCTIKYDNSGNPIWAVRYDGGGDDC
                     AYALVIDGSCNVYITGYSEGSETGLDYTTVKYNSNGEEQWVARYIGPENLDERAFALV
                     IDDSGNVYVTGYSYSPRTRSDYTTVKYNSNGEEQWVAIYNGPVNNEDCAKAIAIDNSG
                     NVYVTGGSYGSGTVSDYATVKYNSNGEEQWMVRYDGPGNDWDEANALAIDSSGNVYIT
                     GFSYGSETENDYATVKYDQNGEEQWVTRYNGSENSHDKGNALVIDGAGNVYVTGYSYG
                     SGTETDYATVKYNSNGEEQWVVRYDGPGNSDDESYALAIDGSGNVYVTGYSYGSGTVT
                     DYATVKYNSNGEEQWVARYNGPESDYYDYDYAYAITVDDSGNVYATGYSHSLGTVEDY
                     ATVKYNSNGEEQWVARYNGPGNSEDKARDIAIDGSGNVYVSGSSYCSQTGYDYATVKY
                     NSNGEEQWTVRYNGPGNSADEAYALAIDGSGNVYVTGYSYGSGTATDYATVKYNSNGE
                     EQWVARYNGPGNSADEAYSMVIDGYGNVYITGFSYGSTTEAEADYATVKYNSNGEEVW
                     VSRYNGPRNFDDEAYSIVIDGSGNV"
     assembly_gap    2652..2751
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2752..3773
                     /locus_tag="PLI55_08060"
     CDS             <2752..3773
                     /locus_tag="PLI55_08060"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPB00829.1"
                     /translation="VTGFSYGSITGADYATVKYNSDGQEQWVARYDGPGNFDDEAYSI
                     VIDGSGNVYVTGFSYGSITGADYATVKYDSNGKEQWVVRYDGIDNSSDYGKAIAIDGS
                     GNVIVTGYSDSLGTRSDYTTIKYNSNGEEQWIARYNGPGNDYDKAYDLAIDDYGNVYV
                     TGESRDLKKGYDYATVKYDSNGEEQWVARYNGPGNDDDKAFAFAINSSGNVYVTGISD
                     GAGWSVYTTIKYSSDGNRIDEEKLKTPVYSLSQNYPNPFNSATTITYSISSQSLVKIE
                     VYNIIGQKVLTLEDGIKTPGVYTARFSPRADFTSGIYFYRLDVLDMSNKSKRFSEIRK
                     MVLIK"
     gene            complement(4033..4767)
                     /locus_tag="PLI55_08065"
     CDS             complement(4033..4767)
                     /locus_tag="PLI55_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HPB00830.1"
                     /translation="MKEFKIGCCGFPIEKEQYYREFNCIEINITFYQLPQIVTAEKWR
                     LAAEKQNPKFEFVIKAWQIITHPAGSFTYRRLREKIGAKENYGLFKDTDEVFTAWERT
                     REFALKLGCTKILFQCPPSFTPDEKYLLNLYNFFEKIRKDSEDFEFILEVRNPKWDKK
                     TIQKVCRELNLIHCVDPLYQTSFYGNYRYYRLHGLHIGNRLDYNYTFSDQELEKVLAL
                     CDQELNYVMFNNFTMYSDALRLMRYT"
     gene            complement(4975..5295)
                     /gene="trxA"
                     /locus_tag="PLI55_08070"
                     /pseudo
     CDS             complement(4975..5295)
                     /gene="trxA"
                     /locus_tag="PLI55_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            complement(5388..>5717)
                     /locus_tag="PLI55_08075"
     CDS             complement(5388..>5717)
                     /locus_tag="PLI55_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB00831.1"
                     /translation="DNEQDAQQAVSIFSLISVVPIFLASYVIANPQSLTTIILSFIPI
                     ITPYFMILRMGVMMPPLWEILTSLGILVVSVWLAMLAAGKIFRVAILIYGKRPTFPEI
                     WQWVRAR"
     assembly_gap    5718..5817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5818..6395)
                     /locus_tag="PLI55_08080"
     CDS             complement(<5818..6395)
                     /locus_tag="PLI55_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB00832.1"
                     /translation="MIITRWEYLTRLRSKGFIISTIIIPLIMVASMFIPSVVLNSDDH
                     EVRLIAVVDETGQFAEKLEQKLADKYTLADQTPKYQVIIIKNGDPVKMKREAAELLNS
                     SVISAYLVIPEVVLDSSRVFYYSLNVGNLKDQSELNQTINEIIAEHRLVKAGLDPALI
                     RDLMKNVEFQTIKVASSGQETSSNETLSFIIP"
     gene            complement(6404..>7098)
                     /locus_tag="PLI55_08085"
     CDS             complement(6404..>7098)
                     /locus_tag="PLI55_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HPB00833.1"
                     /translation="RSDLLKVGYLPEERGLYQKMKLKETVVYLARLKGLSLAEANQCV
                     DEYLRRFDLSGYSHRRIQELSKGNQQKVQFIVSVIHKPELLILDEPFAGLDPVNQLLL
                     KEIIAELQGRGATVIFSTHQMEQVEKLCDRICLINKGKPILNGDLRQIKKEYGQPMLL
                     IDYDGKADALADLNLPDMQITDNRLTCRLTGNLNELLLSVMQRVQVTRFELQEPSLEQ
                     IFIELVRGAENS"
     assembly_gap    7099..7198
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7205..8422)
                     /gene="tyrS"
                     /locus_tag="PLI55_08090"
     CDS             complement(7205..8422)
                     /gene="tyrS"
                     /locus_tag="PLI55_08090"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPB00834.1"
                     /translation="MFPPVKEQLEIIKRGVEELISEEEMVRKLENSIKTHQPLRIKQG
                     FDPTAPDIHLGHTVGIRKLRQFQELGHTIVLIIGDYTGLVGDPSGKNETRPRLQYETL
                     MKNAETYENQFFKILDRSKTEIHYNGEWFAKMKFDEIMNLAAKFTVARILERDDFTKR
                     YKEGNPISLHEFFYPLMQGYDSVAIKADVELGATEQKFNLLTARDVQREYGQEPQVIL
                     TLPVLEGIDGGQRMSKSLGNYIGIDDEPDEMYGKIMSIPDNLILKYFTLVTDYSLAQL
                     QEVKRRLNDGQTNPMVLKKELARAIIKMYHSESAARQAEQNFEQIFSKKEIPDDIEEI
                     IIPSSDLPKPLVKLLTECGAVSSNGEARRLIQQGGVRINDEKISDINYAIQNKGEYIT
                     KVGKRRFFKFIVK"
     gene            complement(8425..8886)
                     /gene="smpB"
                     /locus_tag="PLI55_08095"
     CDS             complement(8425..8886)
                     /gene="smpB"
                     /locus_tag="PLI55_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPB00835.1"
                     /translation="MPKNDVKIITTNKKAFHDYFILDKIEAGIELKGTEVKSVRAGHI
                     NLKDSYAKIIDQELYLLKCHIAPYDFGGYDNHNPERDRRLLLHRNEIRRLQREVENKG
                     VTLIPLRVFITDKGKVKVEIGLAKGKRQYDKRNALAEKEARREVERLRKGK"
     gene            8973..9056
                     /locus_tag="PLI55_08100"
     tRNA            8973..9056
                     /locus_tag="PLI55_08100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9007..9009,aa:Leu,seq:cag)
     gene            complement(9151..9537)
                     /locus_tag="PLI55_08105"
     CDS             complement(9151..9537)
                     /locus_tag="PLI55_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00836.1"
                     /translation="MRRRNLGILVISLMILFGIGVLFVGQEYSSSSKLMTVRVEGLPD
                     AQSGKNMQDLMKMTEGIESFIFDQNVGFCTFRYDSSIINYKAIEAQFLALGLKITPIA
                     PIEITSIISLPPEQKLLKVHFFQPTE"
BASE COUNT         2710 a         1777 c         1995 g         2835 t
ORIGIN      
        1 cattggtcat tgggcattag tcattagtaa aaaggctgag gctaagaaaa aaagctatac
       61 tgtcactctg agcctgtcga agagggacag gatcgaatac cctatgctac ctgtgtgctt
      121 catccttcga cgagctcatc cttcgacaag ctcaggatga caggaagaat gtcactctga
      181 gccatagtgt cactctgagc ctgtcgaagt gtgatttact atgtcatgct gagtttatcg
      241 aagcatgagt ctaccgtatc atggcgatta ttttgtacca acgcccaagc cttgaaacta
      301 gataaacgat aaccttctta aagtccactt gaagtccaaa aatatccatt aaaagcttga
      361 attaattata aatattcgta atattataaa aagaaaatag catggcagga agtttctctt
      421 ataaaattac tttgaggctc ggcaggaaag tgaatagtgc tgtccactta ccttacttgc
      481 ctgaagatag tctgatgaaa agtttaacga attattgagg tggtatgtta tgaaaagata
      541 ttttgtttcc ttagttgttt ttaccttggc aggattttta gcctatgccc aggagttacc
      601 agcgaatcgt agttcgatag actcaccaca tcattcccga ttttcggaag agcgaatagg
      661 aaaaagtgag cagtttaatc ggcatcctta ttctaaattt ctgatggact tagatgaaca
      721 accttataga catccctgtc cttctgagtc tcgagatgct acttctactc tcaatctaaa
      781 taataccgcc actactaata aggaaccaga atggataagt tattatgcct ctggacttct
      841 tccatctcca gatatagcga ctagtattgc cgttgataaa catggtaatg tctatgttac
      901 tggttatagc gtagaggcac tgtttggagc agattattgt actataaaat atgataattc
      961 tggtaatcca atctgggcgg ttagatatga tggcggtgga gatgactgtg cctacgctct
     1021 tgttattgat ggctcttgta atgtttatat tacgggatat agtgaaggtt cagaaacagg
     1081 tctagattat actactgtaa aatataactc aaatggtgaa gaacagtggg tagctagata
     1141 tattgggcca gaaaatttag atgaaagagc attcgctctt gtgattgatg attctggtaa
     1201 tgtttatgtt accggatata gttatagtcc aagaacaagg tctgattata cgactgtaaa
     1261 gtataattca aatggcgaag aacagtgggt agccatatat aatggaccag taaataatga
     1321 agactgtgcc aaggctattg ctattgataa ttctggcaat gtttatgtta ctggaggaag
     1381 ctatggttca ggaacagtat ctgattatgc taccgtgaag tacaattcca atggtgaaga
     1441 gcagtggatg gtcagatatg atgggccagg aaatgattgg gatgaagcaa atgctcttgc
     1501 gattgatagt tctggcaatg tttatattac aggatttagt tatggttcag aaacggagaa
     1561 tgactatgcg acggtgaagt acgatcaaaa tggtgaagag cagtgggtaa caagatataa
     1621 tggttcagaa aattctcatg acaaggggaa tgctttagtg attgatggtg ctgggaatgt
     1681 ttacgttacc ggatatagct atggttcagg aacagaaact gattatgcta ctgtgaagta
     1741 caactcaaat ggtgaagagc agtgggtggt cagatatgat gggccaggaa attctgatga
     1801 cgagtcttat gctcttgcta ttgacggttc tggaaatgtt tatgttaccg gatatagcta
     1861 cggttcagga acagtaactg attatgctac cgtaaaatac aactcaaatg gtgaagagca
     1921 atgggtagct agatataatg gtccagaaag tgattattat gattatgact atgcctacgc
     1981 tattacggtt gatgactctg gtaatgttta tgctaccgga tacagtcata gcttaggaac
     2041 agttgaagat tatgcaactg taaaatacaa ttcaaatggt gaagaacagt gggtagctag
     2101 atataatggt ccaggcaatt ctgaagacaa ggctcgtgat attgcgattg atgggtctgg
     2161 caatgtttat gtttcgggaa gtagctattg ttcacaaaca gggtatgatt atgcgactgt
     2221 gaagtacaac tcaaatggcg aagagcagtg gacagtcaga tataatggac caggtaattc
     2281 tgctgacgaa gcctatgctc ttgctattga cggttctgga aatgtttatg ttaccggata
     2341 tagctacggt tcaggaacag caactgatta tgcgaccgta aaatacaact caaatggtga
     2401 agagcagtgg gtagctagat ataatggtcc aggaaattct gctgacgaag cctattctat
     2461 ggttattgat gggtatggca atgtttacat tactggattc agttatggtt caacaacaga
     2521 ggcagaggca gattatgcga ctgtgaaata taattcaaat ggagaagaag tttgggtatc
     2581 aagatataat ggaccaagaa attttgacga cgaagcctat tctatagtta ttgacggatc
     2641 tggcaatgtt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacgttaccg
     2761 gatttagtta tggttcaata acaggggcag attatgcgac tgtgaagtat aactccgacg
     2821 gacaggagca gtgggtagcc agatacgatg gaccaggaaa ttttgacgac gaagcctatt
     2881 ctatagttat tgatgggtct ggcaatgttt acgttaccgg atttagttat ggttcaataa
     2941 caggggcaga ttatgcgact gtgaaatatg attcaaatgg taaagagcaa tgggtagtca
     3001 gatacgatgg gatagataat tcttctgact atggcaaggc tattgctatt gatgggtccg
     3061 gcaatgttat tgtcaccgga tatagtgata gtttaggaac aaggtccgat tatacgacta
     3121 taaagtataa ttcaaatggt gaagaacagt ggatagccag gtataatgga ccaggaaatg
     3181 attatgacaa agcttatgat cttgctatag atgattatgg aaatgtttac gttactggag
     3241 aaagcagaga tttaaaaaaa ggttatgatt atgctactgt gaaatacgat tcaaatggtg
     3301 aagagcaatg ggtagcccga tataatggac caggaaatga tgatgacaaa gctttcgctt
     3361 ttgctattaa tagttctggc aatgtttatg ttacgggaat tagtgatggt gcaggttgga
     3421 gcgtttatac cacgattaag tatagctcgg atggaaatag gattgatgaa gaaaaactaa
     3481 aaacgccggt ttattctcta tcccagaact atcccaatcc gttcaactca gctactacta
     3541 taacctattc gatcagttca cagtcactag ttaagatcga ggtctacaat attattggtc
     3601 agaaagtact cacgcttgaa gatggtatta agactccagg agtttatact gcgaggttca
     3661 gtcctcgcgc agattttacc agtgggatat atttttaccg gttagacgtt ctggatatga
     3721 gtaataagag caagcgcttt agcgaaatca ggaagatggt gttaattaag tgaagaatag
     3781 gcttgatgtt cttggtcgct agcgttatta attttaactg ctcgtggttt agtagattta
     3841 gtgtccctct aaaaaatgtc accctgagtt tatcgaaggg tgagtttatc gaagggtgag
     3901 tttaccgaag cattgcgatt tatttagcac caacgctcaa gtactggcca gaaaaggaac
     3961 aatgccaagt tcaaccaaca gccacatagt aacgtgctat ttgtagaaaa agaagtgaaa
     4021 ccaaaaatta agttaggtgt acctcattag tcttagagca tcagaataca tagtgaaatt
     4081 attaaacata acataattga gttcttgatc acatagggcg agcacttttt ctaattcttg
     4141 atcgctaaaa gtataattat aatctaatct attcccgatg tgtagtccgt gtaaacgata
     4201 atagcggtaa tttccataaa aactggtttg atataatgga tcaacgcaat gaatcaggtt
     4261 taattcccga catacttttt ggatcgtttt cttgtcccat ttgggattgc ggacttccag
     4321 aatgaattcg aaatcttcac tgtcttttct aatcttctcg aaaaaattat acaggttcag
     4381 caaatatttc tcatctggag taaaacttgg tggacattgg aatagaatct tagtgcaacc
     4441 caatttcaag gcaaattctc gagttctctc ccaggcggta aagacctcat cggtatcttt
     4501 gaacaatccg taattctctt tagccccaat tttttctctc agtcggcgat aagtgaacga
     4561 accagccgga tgagtaatga tttgccaggc tttaatgacg aactcgaatt tcggattctg
     4621 cttttcggcc gccagacgcc atttttcggc ggtgacaatt tgaggcaatt gataaaaggt
     4681 gatgttaatt tcgatacaat tgaattcacg gtaatattgc tctttttcta tcggaaagcc
     4741 gcaacaaccg attttgaatt ctttcatatc taaaatgtag tcaaatcctt actaaaaagt
     4801 gacaaagaat ttctaagttt gtcgaaagcc ctttccctgc aagatcacga ttttcaaaat
     4861 cctcccctta gccgacaatg aaaatattgg tttttcaggt taaatattag taattaattt
     4921 aacgaaaatt aataattaaa cctttgtgcc ttcgtgtctt agtggtaaga aaacttagat
     4981 tacttccagg attttttctt cgatattttt tttaggttgg gcgccggtaa tctgagccat
     5041 tattttacca tttttgaaaa tcagcagagt cggaatactg cggatgccga atttctgagc
     5101 aatttgcggg gaattttcga cattcacttt gccaaatttc attttgccgt tatacttttg
     5161 cgccagttcc tcaattaccg gcatcagttt caaacaagga gcgcaccagg ttgcccaaaa
     5221 atcaaccagc accggtaaat cggattgctc gacttcagcc ttgaaatttg attgagtgaa
     5281 ttctaccata aaaccccgct tttttactac ttgattatct caacgtttca cgctataata
     5341 tattcttaac tacttttaat ctcaaatcta ttttttccgg aaatttttta tctggcgcgc
     5401 acccattgcc atatttccgg aaacgtaggt cttttgccat atattaaaat cgccactctg
     5461 aaaattttcc cagccgccag catcgccagc caaacactta caaccaatat tcccaacgaa
     5521 gtcaagattt cccaaagcgg cggcatcatt acacccattc ttaaaatcat aaaatagggc
     5581 gtgatgatcg gaatgaacga taaaattatc gtcgtcaacg actgaggatt ggcaattacg
     5641 taggaagcca aaaaaatcgg tacaaccgag atcaacgaaa aaatcgaaac ggcttgctga
     5701 gcgtcttgtt cattgtcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag
     5821 ggaataataa atgagagggt ttcgttactc gaagtctcct gtccgctgga agcgactttt
     5881 atggtctgga attcgacatt tttcatcaga tctcggatta aagccggatc taaaccagct
     5941 ttaaccaacc gatgctcggc aataatttca ttgatcgttt ggttaagttc actttgatct
     6001 tttaagttgc cgacatttaa gctataataa aacacacggc tggaatccag tacaacttct
     6061 ggaataacga gataagcact tataactgaa gaatttaaaa gctcagccgc ttctcgtttc
     6121 attttcacag gatcaccatt ttttattatg ataacttgat atttcggggt ctgatccgca
     6181 agagtatatt tatcagccaa cttttgttcc aatttttccg caaattgacc agtttcatct
     6241 actacggcaa tcaggcgcac ttcatgatcg tctgagttta acacgactga tggaatgaac
     6301 atcgaagcca ccataatcag cggaataata atcgtcgaga tgataaatcc tttggaacgc
     6361 agacgagtta ggtattccca gcgggtgatt atcattatat ttttcatgaa ttttccgccc
     6421 ccctcacaag ctcgataaag atttgctcca gagatggctc ctgcaattca aaacgagtta
     6481 cctgcacacg ttgcattact gagagtaata attcattgag attaccggta agacgacagg
     6541 taagtcggtt gtcagtgatt tgcatatcag gcaaattcaa gtcagccagc gcatccgcct
     6601 tgccgtcata atcaatcaaa agcattggtt gaccatattc tttcttaatc tgccgcaagt
     6661 cgccgttcag aattggttta cctttattta tcaagcaaat ccggtcgcat agcttctcga
     6721 cttgttccat ttggtgagtc gaaaatatta cggtcgcgcc gcgtccctgc aattcagcaa
     6781 taatttcctt caatagcagc tgatttaccg ggtcgagacc agcaaaaggt tcgtccagga
     6841 taagaagttc cggtttgtga attaccgaaa caatgaattg gactttctgt tgattgcctt
     6901 tggaaagttc ttgaatgcgg cgatggctat aaccagagag atcgaaacgc cgcaggtatt
     6961 cgtccacgca ttggttagct tctgccagag atagaccttt gaggcgggca agataaacga
     7021 cggtttcctt caatttcatt ttctggtaca atccgcgctc ttccggcaaa taaccaactt
     7081 tcaataaatc agaccgctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnag
     7201 gtgattattt aacgatgaat ttgaaaaacc gccgtttccc gactttggtt atatattcgc
     7261 ctttattttg aattgcataa ttgatatcgc taatcttttc gtcgttgatg cgtacgccgc
     7321 cctgctgaat taggcggcga gcttcaccgt tagaagaaac cgctccgcac tcggtaagta
     7381 atttgactaa aggttttggt aaatccgatg agggaattat tatttcctcg atgtcgtcgg
     7441 gaatttcttt cttactgaaa atttgttcga aattttgttc agcctgacgg gcggcggact
     7501 ctgaatgata cattttgata attgcgcggg ctaattcctt ttttagcacc attggattgg
     7561 tctgcccgtc atttaggcgg cgctttactt cctgaagttg ggcaagcgaa taatccgtca
     7621 ccagagtaaa atatttaaga atcagattat cgggaatgga cataattttg ccgtacatct
     7681 cgtccggttc atcgtcaatg ccgatataat taccgagact tttgctcatt cgttgaccac
     7741 catctatgcc ttccagcacc ggcagagtta atataacctg tggttcctga ccgtattcgc
     7801 gttgaacgtc acgcgcggta agcagattga atttctgttc ggtggcgccg agttcgacat
     7861 cggctttgat tgccacggaa tcgtagcctt gcatcaatgg atagaagaat tcgtgcagac
     7921 taatcggatt gccttcttta taacgtttgg taaaatcgtc tctttcgagg atccgtgcaa
     7981 cggtaaattt cgccgccaga ttcataattt catcgaattt cattttggcg aaccattcac
     8041 cgttatagtg gatttcggtt ttacttctat ccagtatttt aaaaaattga ttctcgtaag
     8101 tttcggcatt tttcatcaag gtttcatatt gcaatcttgg tcgcgtttcg ttcttgccac
     8161 tcggatcacc aaccaagccg gtgtaatcgc cgataataag gacgattgta tgtcccagtt
     8221 cttggaactg acgcaatttc cggattccga ccgtatgccc aagatgaata tccggtgcgg
     8281 ttggatcgaa gccctgcttg attctaagcg gctgatgggt tttaattgaa ttctcgagct
     8341 ttctgaccat ttcctcttcg gaaattaatt cctcgactcc tcgtttgatg atttccagtt
     8401 gttctttaac cggtgggaac atgcctattt tccttttctt aaacgttcaa cttcgcggcg
     8461 cgcttctttc tcagctagcg cgttcctctt atcgtactgt cttttgcctt tggctaaacc
     8521 tatttcgact ttaactttcc ccttatccgt aataaaaacg cggagcggaa tcagggttac
     8581 gcctttattc tcgacttcgc gttgcaggcg ccgaatttca ttccggtgca atagcagacg
     8641 gcgatcgcgt tccggattat gattgtcgta tccgccaaaa tcgtagggag caatatgaca
     8701 tttcaataaa tataattcct gatcaatgat tttggcgtaa ctgtctttga gattgatgtg
     8761 cccggctcta accgatttga cttcggtgcc tttgagttcg atgccagctt caattttatc
     8821 caggataaaa taatcgtgga aggctttctt attagtcgtt ataattttga catcattctt
     8881 tggcatacgg aaaatttaat tcttttgcct caacttccaa tgtaaaactt ggagaactca
     8941 gtcggcggtt ataaattttg ccgttatttg aagcccgggt ggtggaactg gtagacgcgc
     9001 taggttcagg acctagtctc cattaggagg tgcgagttca agtctcgccc cgggcacaaa
     9061 gattgaataa tgcgaaatta ggcttaatta cttatatcgg taaccaacgc tgttcagttt
     9121 ggaattatct cgctaactcg gcaatgtctg tcattcggtc ggctgaaaaa agtgaacttt
     9181 cagcaatttt tgttccggag gcagagagat aatactggtt atttcgattg gagcaatagg
     9241 cgttattttc agaccaagcg ccaaaaactg agcttcgata gctttgtaat ttataatcga
     9301 ggaatcgtaa cggaaagtgc aaaatccgac gttttgatcg aaaatgaacg attcgatgcc
     9361 ctccgtcatt ttcattaaat cctgcatgtt tttacccgac tgcgcatccg gtaaaccttc
     9421 gacccgaact gtcattaatt tactgctgga tgaatactcc tgcccaacga aaagcacacc
     9481 gataccaaac aggatcatca acgaaatcac caaaatgccc aaattgcgtc gcctcataca
     9541 aatgcttcct tttagctact accccaaaaa aagcgatata atttaaggac ttactaacta
     9601 aaatgaaagt aatataa
//
LOCUS       DAOOMU010000084         9445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_8468_length_9445_cov_15.803621, whole genome shotgun sequence.
ACCESSION   DAOOMU010000084 DAOOMU010000000
VERSION     DAOOMU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8468_length_9445_cov_15.803621"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            289..2358
                     /locus_tag="PLI55_08110"
     CDS             289..2358
                     /locus_tag="PLI55_08110"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB00837.1"
                     /translation="MDRIVQHPILTIPPARKLVKFTFNDTILEGFEGEPVSSALMAQG
                     YREFSIHRKGDTPQGLFCANGQCSHCTLVIDGLAQKSCVTPLKGGMQIKTLRHIPEIP
                     ADNRPLTHYKKIELTCDILVIGGGPAGLMAALELAQLNYNVIVVDDKEKLGGKLLLQT
                     HKFFGSIEDCYAGRRGIEIAALLEKELSSHTNIKIFTDTTVAAIYKDKKAGLFIKNQN
                     YAIVAFSGLIVSPGAHEKSLIFNGNHLPGVFGAGAFQTLVNRDLVKAGRRIFIVGSGN
                     VGLIAAYHALQAGITVVGICDILDKVSGYKVHADKIERMGVPIYLKHTVIAAEGNGKV
                     ERVIIAAVDDHFQPLLETARTFAVDTLLIAVGLTPLDEFFEMANQFGFPVVKAGDADE
                     IAEASSAMFSGRIAARRLANQLGKNIAIDSTFIQKAEILKNKPGRIFPSTPVVLDEKF
                     QPIIRCTEEIPCNPCTAVCPVNAIQLRGDRHDLMDLPFYQGGCTGCMQCVLICPGLAI
                     TLARKIDEQFGEVVLPHEYLATFKVGDRLELTGVSGEFLESGEILKIRYFKKHKTYLI
                     HIKTTLQNAPLVAGIRVQPESETTALPEAQFKYLPDDGIVCHCELVTVKEIRDFIREH
                     NVYDVNQLKLIRVGMGACGGKNCSVLLSRIFEEAGVDFHTVTPGTKRPLSVEIPMSAI
                     VNEESES"
     gene            2359..>2527
                     /locus_tag="PLI55_08115"
     CDS             2359..>2527
                     /locus_tag="PLI55_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB00838.1"
                     /translation="METYDVVIIGAGSIGTPLSYYLTRSGLRVIVLEQLASVGRGQNR
                     AAIGGIRATHSD"
     assembly_gap    2528..2627
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2628..3268
                     /locus_tag="PLI55_08120"
     CDS             <2628..3268
                     /locus_tag="PLI55_08120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPB00839.1"
                     /translation="EPVTGFKWNQNKITTIITTKSTYSCRWVINAAGAYARRIGEMLE
                     LDLPVFPESHEGGITEPVSRFFAPMIVDVRPDSESSNYYFYQNREGQVVFCITPEPKI
                     AGTDCDSTSNFLPLVTKRMLSLYPRLRHLRVRRTWRGLYPATPDGSPIVGFTHQFCNL
                     FLAIGMCGQGFMIGPGLGAIIAEILTDNTHKYDEILDQWNLYRNFTGQEILK"
     gene            3285..4481
                     /locus_tag="PLI55_08125"
     CDS             3285..4481
                     /locus_tag="PLI55_08125"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPB00840.1"
                     /translation="MTIPISKRAQALKPSPTMSVTALASKMAAEGISIINFGVGEPDF
                     NTPENIKQAAHQALDANFTHYTVSAGILELRQAICDKLKRDNNLDYSPDEIIVAPGGK
                     AAIITTLAAICDPDDEIIIPAPYWVSYPSQVEMLDGKPVFIATDESTNFKITADQLES
                     VIRNISQPKALILNSPNNPTGAVYSPTELAAIGAVCLKHNLAVISDEIYEKLVYGSAQ
                     HYSIVQVLPEIRSITIVINGFSKAYAMTGWRVGYAAGPREVIKRATAIQEHTASCVTS
                     ITQKAAVAALNGDDSSIEAMRQEFERRRDYLVNELKQIPLVDCPLPDGAFYAFPNISH
                     YIKNNKIGIRDSVQLCEYLLKNFHIALVPGSAFGCDDCLRFSYATSMENIVEGVKRFK
                     SGLASL"
     gene            complement(4610..>5727)
                     /locus_tag="PLI55_08130"
     CDS             complement(4610..>5727)
                     /locus_tag="PLI55_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00841.1"
                     /translation="GGGLLRFNLDDLENYVVYDTTGGKLAGSAGYGGRDDYLGIAYMA
                     LDNQNNLWIANQYAQNDNFIAVLTPDDRWVHFSGQESGNYLNYLVTSIAFDAAGRVWF
                     ASEATSEPPGSNGGIAVLDYKNTLFDKSDDQWYRVTTSDGLASNMVYSLAFDLENKLW
                     IMTAGGIQSASVSESFPARVFDAIDYAALTNIAFAKECRIKVDGLNNKWITTVNSGVK
                     LYTYNGIWLNDKEGFTSKNSGLLSDNILDIAFDPPRGLVYISTDKGISVYKSPYAYYG
                     NQYQKPNTFPSPFRIPADQPLTIDGLLQDSEVKIMLLDGTFVRHLDPKDGEVVGQQAY
                     WDGRNEQGKLVSSGVYIFVAYTPDGDTITGKIAVVRY"
     assembly_gap    5728..5827
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5828..6816)
                     /locus_tag="PLI55_08135"
     CDS             complement(<5828..6816)
                     /locus_tag="PLI55_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00842.1"
                     /translation="MRRLLILLAVPLIIQAQSIVGEWSSYTSIISHRELTQVGDFIYI
                     ASPGGLVEFNKTSSKFKVYSPNDGLSFTDINCLGLDELGDIWLGMSSPNGEVNIWSPV
                     TRQCKKVINAAELREELTLISAITFTADKAYVAYQKNITWGIMYFKIDKNEYTYIDFC
                     ENFPAEFFKINKLVAINDTLWVATDAALFFADLQENFPKGPNNWEVVDLIGQGNITSI
                     IEYDEEVICSCGNYIYGIKGTSAVVVDSLASSIVQLTEDQDGNLIAATTGGIFRLFNG
                     VWQIISQNAVNQLYIDDDGIIWGATSSRGLLKIENGSEKFFVPNSLLDNGNTSL"
     gene            complement(6820..7299)
                     /gene="ribE"
                     /locus_tag="PLI55_08140"
     CDS             complement(6820..7299)
                     /gene="ribE"
                     /locus_tag="PLI55_08140"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPB00843.1"
                     /translation="MKLHEGVLSAEKMKVGIVASRFNDMITKELLAGALDCLKRHGVD
                     ENDIHVYWVPGALEIPRIARELADSGQFDGVVCLGAVIRGATPHFDIVANEASKGIAR
                     LSMESDVPVIFGVLTTENIEQALERAGTKAGNKGWDAALNMIEMANLIRSIRVHSQK"
     gene            complement(7295..8515)
                     /locus_tag="PLI55_08145"
     CDS             complement(7295..8515)
                     /locus_tag="PLI55_08145"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPB00844.1"
                     /translation="MKFDSIDAAVEDLKKGRFVIVVDDENRENEGDFIMAAEKVTPES
                     VNFMAKYARGLLCVGLTEERAKELNLELMVQDNTALHNTRFTVSVDLKEGTTTGISAN
                     DRALTINALANPNTKPEDLGRPGHIFPIIARPGGVLRRAGHTEASVDLMRLAGLTPVS
                     VMCEIMDENGEMARGEALEKIAQTHNVKIITVQSLIEYRRRTERFIERVSEADLPTKF
                     GVFRIIIYVDRLTDKEHVALVMGDIKSDEPVIVRVHSECLTGDVFSSERCDCGDQLHK
                     ALEIIAQNKRGVLLYLRQEGRGIGLRHKISAYKLQDEGMDTVEANIKLGFLPDLRDYG
                     MGAQILADLGVHKMRLLTNNPKKIVGLQGFNLEVVERIPIEIPPNPQNEKYLKTKRDK
                     MGHLILNNKKVPND"
     gene            complement(8542..9183)
                     /locus_tag="PLI55_08150"
     CDS             complement(8542..9183)
                     /locus_tag="PLI55_08150"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018922156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPB00845.1"
                     /translation="MFTGIIEEMGEIRAVSENVAGRRFQIAANRILNGINIGDSIAID
                     GVCLTVENFGKDYIEVQAVTETLARTTLAKVPRGAKVNLERAMAASGRFGGHFVQGHI
                     DSVAPIVDLQRIGNAATMRIAVPDSIRHYIVEKGSLAVNGVSLTVAGIENEVIEIALI
                     PVTLTETNLGEKKIGDEVNLEVDILAKYIEKILEHFEKETLTFEKIKQWGFNQ"
     gene            complement(9183..>9445)
                     /locus_tag="PLI55_08155"
     CDS             complement(9183..>9445)
                     /locus_tag="PLI55_08155"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HPB00846.1"
                     /translation="DLPKTLAKLAEFGVTSIFCEGGSQVAGSLLNQRLVDELQVFIAP
                     KILGEGLRAFSGFMQSLDQALIVEWEKVERLGADVLLQGKLV"
BASE COUNT         2570 a         2250 c         1863 g         2562 t
ORIGIN      
        1 agctcaggat gacaggaaca gacaagctca ggatgacaag aacggacaag ctcatccttc
       61 gacaagctca ggatgacagg agaaatgtca ctctgagcct gtcgaagagg gagtttatcg
      121 aaatggtact agttacagtt ttcaggaaac aggtttagaa tagcccgtaa atttattcgt
      181 aaatttttta ataatatttc ctcattaaat attttcatta agccaccgta tatctaaatt
      241 tgaccaatta ttttatgatt acaaatcctg ttaaatacaa aggcgaaaat ggacagaata
      301 gttcagcatc cgattttgac gattccaccc gctcgaaaac tagtaaaatt tactttcaat
      361 gatactattc tggaaggctt tgaaggcgaa ccggtttctt cggcgctgat ggcgcagggc
      421 tatcgggaat tttccattca ccgcaaaggt gacacgccgc agggactctt ctgtgccaat
      481 ggtcaatgtt cgcattgcac tttagtcatt gacggtttgg cgcaaaaatc ctgtgtgacg
      541 ccgttaaaag gcggtatgca gattaaaacc ctgcgccata ttccggaaat tccagccgat
      601 aaccgcccac tgacccacta caaaaaaatc gaattgactt gtgatatatt ggtaattggc
      661 ggcggacctg ccggattgat ggccgctctc gagctcgccc aactgaatta taacgttatc
      721 gttgttgacg ataaggaaaa attaggcggt aaacttctac ttcagacgca taaatttttc
      781 ggctcgattg aagactgtta tgctggtagg cggggaattg aaattgccgc tttactggag
      841 aaagaattaa gcagtcacac gaatattaaa attttcactg atactactgt agccgcaatt
      901 tacaaggata aaaaagccgg gcttttcatc aaaaaccaaa actatgcgat tgttgccttt
      961 agcggtttga tagtttctcc cggtgctcac gaaaagtcac tgattttcaa tggcaatcac
     1021 cttccaggag ttttcggtgc tggtgctttt cagaccctgg tcaatcgtga tctggttaaa
     1081 gctggtcgtc gcattttcat tgttggtagc ggcaatgtcg gtttgattgc ggcttatcat
     1141 gctttgcaag ctggaattac agttgtcggc atttgtgaca ttcttgataa agtttccggc
     1201 tacaaggttc acgccgacaa aatcgagcgg atgggcgtgc caatctactt aaaacacacc
     1261 gtaatcgccg ccgaaggcaa cggcaaagtc gagagagtta tcattgccgc ggttgacgat
     1321 cattttcaac ctttattgga aactgctcga acttttgcag ttgatacttt actaatcgcg
     1381 gttggtttaa ctccacttga tgaatttttc gagatggcta accaatttgg ttttccggtt
     1441 gttaaagccg gtgatgccga tgaaatcgcc gaggcttcat cggcgatgtt tagcgggcga
     1501 attgcggcac ggcgtctggc aaatcagctt ggcaaaaata tcgcaataga ttcgactttt
     1561 atccagaagg ctgaaatttt aaaaaacaag ccgggacgaa tttttccatc aactccggta
     1621 gttttggatg aaaaatttca gccgattatc cgttgtacgg aagaaattcc ctgtaatcct
     1681 tgcacggcgg tttgcccggt gaatgccatt caacttcgcg gtgatcgtca cgatttaatg
     1741 gatttaccct tttatcaagg cggctgtacc ggctgtatgc aatgcgtttt aatctgtcct
     1801 ggattggcta ttaccttggc tcgtaaaatt gacgaacaat ttggcgaagt ggttttaccg
     1861 cacgaatatt tagcgacctt caaagtaggc gatcggctgg aattgaccgg agtttcggga
     1921 gaatttctag aaagcggtga gattctgaaa atacgctatt ttaaaaaaca taaaacttac
     1981 ctgatccaca ttaaaaccac cttacaaaat gcgccgctgg tagccggaat cagagtgcaa
     2041 ccagaatctg agacaactgc gttgcctgag gcgcaattta aatatttacc ggacgacgga
     2101 attgtttgcc attgtgaact ggtcaccgtc aaagaaatcc gcgactttat ccgcgagcat
     2161 aatgtttacg acgttaatca attgaaatta atcagagtcg gtatgggcgc ctgcggtggc
     2221 aaaaattgct ccgtgcttct atcacgtatt ttcgaagaag ctggggttga tttccacacc
     2281 gttacgcccg gcaccaagcg cccactcagc gttgaaattc caatgtctgc catcgtcaac
     2341 gaagaaagcg aatcttaaat ggaaacctat gatgtcgtta taatcggagc aggcagtatt
     2401 ggtacaccac ttagttatta tctaacgcgg agcggtctgc gggtaattgt gctggaacaa
     2461 ttggcttcag ttgggcgcgg tcagaatcgc gccgctatcg gaggcattcg ggctactcac
     2521 tctgattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntgg aaccggtcac
     2641 cggttttaag tggaatcaaa ataaaattac gaccattatt actactaaat ccacctattc
     2701 atgtcgttgg gtaatcaatg ccgctggagc ctatgctcgt cgcattggcg aaatgctgga
     2761 actcgatttg ccggttttcc cggagagcca cgaaggcggt attaccgaac cggttagccg
     2821 ctttttcgca ccgatgatcg tagatgtccg accggacagc gaatcaagca attattattt
     2881 ttatcagaac cgcgaaggtc aggtggtctt ttgcatcaca cccgaaccta aaatcgccgg
     2941 aactgactgt gacagtactt cgaactttct acctttagtt accaaaagga tgcttagtct
     3001 ttacccacgc ctgaggcatc tgcgcgtgcg ccgcacctgg cgtggacttt atcctgccac
     3061 gccggacggt tctccgatcg tcggattcac tcatcaattc tgcaatttat ttttggcgat
     3121 tggaatgtgc ggacagggtt ttatgatcgg tcccggtctt ggtgcaatta tagccgaaat
     3181 actaacggac aatactcata aatatgatga gattctcgac cagtggaatc tctatcgtaa
     3241 ttttactggc caggaaattt taaaataaaa taagaggagc ttctatgact attcctattt
     3301 caaaacgagc ccaagcctta aaaccgtcac caactatgtc tgtaaccgct ttggcttcaa
     3361 aaatggcggc tgaaggcatt tccataatca actttggggt cggcgaacca gattttaata
     3421 ctccggaaaa tatcaaacaa gccgctcatc aggcgctgga tgccaacttc actcattata
     3481 ccgtctcagc cggcattctg gaattacgcc aggcaatttg tgataaactc aaacgtgata
     3541 ataatctgga ctattcaccc gacgaaatca tcgtagcacc cggcggaaaa gccgccataa
     3601 tcaccaccct cgccgcaatt tgtgaccccg acgatgaaat tatcattccc gccccctatt
     3661 gggttagtta tccttcccaa gtcgagatgt tggatggcaa gccggttttc atcgccaccg
     3721 acgaatcaac caatttcaaa attaccgccg accagctgga atcggttatt agaaacattt
     3781 cgcaaccgaa agcactaatc cttaattcac cgaacaatcc aaccggcgcc gtttattcgc
     3841 caaccgaatt agccgctatc ggcgcagttt gtctaaaaca caatttggca gtgatctctg
     3901 acgagattta cgaaaaatta gtttacggtt ccgctcagca ttattcgatc gtccaggttt
     3961 tgcctgaaat ccgatccatt acaattgtca taaacggctt cagtaaagcc tatgcgatga
     4021 ctggctggcg cgtaggttat gccgccggac cgcgtgaggt catcaagcgg gcaaccgcca
     4081 ttcaagagca taccgcctcc tgcgttactt caatcaccca aaaagccgca gttgccgctt
     4141 tgaatgggga tgacagcagt atcgaggcta tgcgccagga atttgaaaga cgccgtgatt
     4201 atttagtaaa tgaattgaaa cagattccat tagttgactg cccgttacca gatggcgcat
     4261 tttacgcgtt cccgaatatt tctcactata taaaaaacaa caagatcggc atccgtgatt
     4321 cggttcaatt atgtgagtat ttactgaaaa atttccacat cgctttggtc cccggttcgg
     4381 cttttggttg cgatgactgc ctgcgctttt cctatgccac cagtatggaa aatatcgtcg
     4441 aaggggtcaa acgtttcaaa tctggattgg caagtttata gacatcaggg ctttcctcaa
     4501 tttttgggaa tattcaatcc ctccaaggca aatatatttt gtaaggagat aattatctat
     4561 tatactctaa aatctaccgc accaattatt tatttctttg atgaattttc taatagcgaa
     4621 cgacggcaat tttaccagta atcgtatcgc cgtctggagt ataggcaaca aagatataga
     4681 cgccactgct gactaatttt ccttgttcat tccgcccatc ccagtaagct tgttgtccga
     4741 ctacttcccc atccttagga tccaggtgtc ggacaaacgt gccgtctaga agcattattt
     4801 tcacttccga atcctgtaac aatccgtcaa tagtcaaagg ttggtcggca ggaatccgaa
     4861 aaggcgatgg gaaagtgttc ggcttctggt attgattgcc ataataggca taaggcgatt
     4921 tatacacgga aatacctttg tcggtagaaa tatacactaa gccccgcggt ggatcgaaag
     4981 ctatatcgag aatattatcg cttaacaacc cagaattctt actggtaaat ccctctttat
     5041 cgtttagcca gataccatta taagtatata attttactcc ggaatttacc gtcgtaatcc
     5101 acttattatt taagccatca accttgatgc ggcattcttt ggcaaaggca atattggtca
     5161 aagccgcgta atcaatggcg tcgaagactc tagctgggaa actttccgaa acgctcgctg
     5221 attgaatacc gcccgctgtc attatccaca atttattttc cagatcgaat gccagagaat
     5281 aaaccatatt gctggcgaga ccgtcgctgg ttgttaccct gtaccattga tcatcacttt
     5341 tatcaaataa agtatttttg taatcaagca cggcaattcc gccgttggaa ccaggtggtt
     5401 ccgaagtggc ttcagaggca aaccacactc tgcccgccgc gtcaaacgcg attgaagtaa
     5461 ctaaataatt caaataattc cctgactctt gcccgctaaa atgtacccaa cggtcatcgg
     5521 gcgtcaaaac cgcaatgaaa ttatcgtttt gagcatattg attggcaatc cataaattgt
     5581 tctgattatc aagcgccata taagctattc ccaaataatc atctcgccca ccataacccg
     5641 ctgaaccagc cagtttaccg cctgtagtat cgtaaacgac ataattctcg agatcatcta
     5701 aattaaatct caacaaaccg ccgccgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5821 nnnnnnnagc gacgtattgc cgttatccaa gagcgaatta ggaacgaaaa atttttcact
     5881 cccattctca attttcaata agcctctgga actcgtagcg ccccaaataa ttccatcatc
     5941 gtcaatataa agctgattga cagcattctg actgatgatt tgccagacgc cgttaaaaag
     6001 acgaaaaata ccaccggtcg tcgccgcaat taaattacca tcctggtctt cagtcaattg
     6061 cacgatggaa cttgccaagc tatcaacaac aaccgctgaa gtacccttaa tcccgtaaat
     6121 ataatttccg caggagcaaa tcacttcctc gtcatattcg attatactgg taatgttccc
     6181 ctgcccgatt aaatccacca cttcccaatt attaggtcct ttaggaaaat tctcctgcaa
     6241 atcggcaaaa aacaaagccg catcggtagc tacccatagc gtatcgttaa ttgcaactaa
     6301 cttgttaatt ttaaaaaact ctgccggaaa attttcgcaa aaatcaatgt aggtgtactc
     6361 gttcttatca attttaaaat acattattcc ccaggtgata tttttttggt aagccacata
     6421 ggctttgtcc gcggtgaagg tgatcgctga aataagagtc aattcttcgc gtaactcggc
     6481 ggcattaatt actttcttac actggcgcgt aactggactc cagatattga cctcgccatt
     6541 tggtgaactc atccccaacc agatatcgcc aagttcatcg agtcccagac aattaatatc
     6601 ggtaaatgat aaaccatcat tcgggctata cactttgaat ttcgaactgg ttttattgaa
     6661 ctcgactaat ccaccgggac tggcaatata gataaaatca ccgacctgag tcaattctct
     6721 atggctaata atcgaagtgt aactactcca ttcccccact atgctctgag cttgaataat
     6781 cagcggcaca gctaaaagta ttagcaatcg tcgcatagtt tacttctgac tatggacacg
     6841 aattgaacga atcaaattcg ccatctcaat catattgaga gcggcatccc aacctttgtt
     6901 accggctttc gtgccagcac gttcgagagc ttgctcgata ttttccgtcg ttaggacacc
     6961 gaaaatcacc ggcacgtctg attccattga tagtcgagcg attcctttgg aagcctcatt
     7021 ggcaacgata tcaaaatgag gtgtcgcgcc acgaataacg gctcctagac aaacaacacc
     7081 gtcaaactgg ccagaatcag ctaattcgcg agcaatgcgc ggaatttcaa gcgcgcctgg
     7141 cacccagtaa acgtggatat cattttcatc aacgccatgc cgtttcaaac agtccagcgc
     7201 accggcaagc aattctttag taatcatatc gttaaaccga ctggcaacga tcccgacttt
     7261 cattttctcg gcggaaagca cgccttcgtg tagtttaatc atttggcacc tttttattat
     7321 ttaagattaa atgtcccatt ttgtcccgtt tagttttgag atatttttca ttttggggat
     7381 ttggtggaat ctcgatcgga atgcgctcca ctacttcgag attaaatccc tgcaagccaa
     7441 caattttctt tgggttattg gtcagcagtc gcatcttatg tactcccaga tcagccagaa
     7501 tttgggcacc catgccgtaa tcacgcaaat ccggcagaaa acccaatttg atattagcct
     7561 cgactgtatc catgccttca tcctgcagtt tataagccga aattttatga cgtaaaccga
     7621 ttccgcgtcc ctcctgccgt aaataaagca agacaccgcg tttgttctgc gcgattatct
     7681 ccagcgcttt gtgcaactga tcaccgcaat cacaccgctc tgagctaaaa acatcgccag
     7741 tcagacattc agaatgcacc cgaacaatca ccggttcatc cgattttata tctcccataa
     7801 ccaaagccac atgttcttta tcggtaagcc ggtcaacata aataatgatt cgaaaaacgc
     7861 caaattttgt cggcaaatcg gcttcactca cccgctcgat aaagcgttcc gttcttcgtc
     7921 ggtattcaat caaactctga acggtaataa ttttaacgtt atgagtctgg gcgattttct
     7981 ccaatgcttc accacgcgcc atttcgccat tttcgtccat aatttcgcac atcacactga
     8041 cgggagtcaa tccagccaat cgcatcaaat ccactgaggc ttctgtatga ccagcacgtc
     8101 gcaggactcc acccggtcgc gcaataatcg ggaaaatatg tcccggacgc cctaaatctt
     8161 ccggtttggt gttcggatta gcgagagcat taatcgtcaa agcgcgatca ttagccgaaa
     8221 ttccggtcgt cgtgccttcc tttaaatcca cactaaccgt aaagcgagta ttatgcagag
     8281 cggtattatc ctggaccatc aattctaaat taagttcttt cgctcgctcc tccgtcaaac
     8341 ctacacacag cagaccgcgc gcatattttg ccataaaatt gaccgattct ggcgttactt
     8401 tttcagccgc catgataaaa tcgccttcgt tttcgcgatt ttcatcatca acgacgatga
     8461 caaagcgccc cttttttaga tcttcgacgg cggcatctat tgagtcaaat ttcatcaaat
     8521 cctcttaata tcttaaaatt gctattgatt aaatccccat tgtttaattt tttcaaaagt
     8581 tagcgtttct ttctcaaagt gctcaagaat tttttcaata tacttagcta atatatcaac
     8641 ctcaaggtta acttcgtcgc caattttttt ctcgccgaga ttagtttcgg tgagcgttac
     8701 tggaatcaag gctatttcaa ttacctcatt ctcaattcca gccaccgtca aactaacacc
     8761 attaaccgcc aaagagccct tctcgacgat ataatgccga atcgaatccg gtacggcgat
     8821 tcgcatagta gccgcattgc caattcgctg taaatcaacg atcggcgcaa cgctatctat
     8881 gtgcccttgg acaaaatgtc ctccaaaacg tccacttgcc gccatagcgc gttccagatt
     8941 aacttttgca ccacggggca ccttagccaa cgtcgtccgc gcaagtgtct cagtgaccgc
     9001 ctggacttca atataatctt taccgaaatt ttcgactgtc aaacaaacgc catctatggc
     9061 gatgctgtcg ccgatattaa tgccgtttaa aattcgattc gccgcaatct ggaaacggcg
     9121 tcctgcgaca ttctcggaga cggctcgaat ttcacccatt tcttcgataa ttccagtaaa
     9181 cattagacta atttcccttg tagaagcaca tccgctccaa gacgctcgac tttttcccat
     9241 tcaactatca aagcctgatc cagtgattgc ataaatccgg aaaacgcccg caaaccttcc
     9301 ccgagaattt tcggtgcaat aaatacttgt aattcatcca ctaggcgttg attcaacagc
     9361 gatcccgcca cctgactacc gccttcgcaa aaaatggaag taacgccaaa ctcagccagt
     9421 ttagcaagag ttttcggcaa atcca
//
LOCUS       DAOOMU010000085         9375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_8553_length_9375_cov_4.201073, whole genome shotgun sequence.
ACCESSION   DAOOMU010000085 DAOOMU010000000
VERSION     DAOOMU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8553_length_9375_cov_4.201073"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..2430
                     /locus_tag="PLI55_08160"
     CDS             112..2430
                     /locus_tag="PLI55_08160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB00847.1"
                     /translation="MKNLKKLFFIFLITGTYINLYSQVREYRIHNRGILHETVFNTGE
                     IGRAWQTGEAGNETSVPLMEWPSNSRTILDGIEYSGQHNIIGAGVYIGANKEGLPGKS
                     NRLFALCGAVGASDPEVAIGRWSFPLYMEEIENFPLNSDGSLNENYNPNEAEEIISAG
                     WSTPTGISVYRTSRAWSYPDYDDLIIYEYEFEYTGDTDGDPSTIEMNDKLKDVMICFN
                     YGLYPSMYGAQRVYLEWKYEGGLYRGDVNNFWDADYWLTWNMNLRNNITDENGMGKPE
                     PNPDYFLQFARSGEHGGGLNSPQAPGYCVLFYDTTHLAVVIPPEMDTLGLNESEAQAI
                     LRSTTVTSLSQYSGIDVYTETTGGYKWYYELDKSFHIRQPWDNKVSTGNTNSEKIMYE
                     KEPFNPSTRWSGVYSPGSATWPEVKSDDWYGRAAYPYRQSADAGMRLHAFGPYTLKPG
                     DKIRLAYAEVIGYGAQEGKRVEGGQTATQWAKIPSLNRKVTYKDSKGNEKVLTEHYLD
                     DFGYPDYVNSNIITVHDVARKAFEAYLGNEPVVPVQPEKHPSRGTYSVPTPPPAPVIS
                     IKNYGLAEVKITWGRAQEQFTHPRLTGDLAKYMVQRAVSGMGPWITLDTIDVGDNEYL
                     NENDEYEYIDSDPTYNVGEFRYYAVVSVDEHGNKSGKTNMTCFQKNIPAVEKIDKVYV
                     VPNPFICKSGFTTEGDERMIGFYGLPDVCTIRVFSYSGQLIDTIEHKNSAYSHEKMLV
                     TRNNQDMASGIYFYVVTTPEGEKSFGKFIILK"
     gene            2452..3426
                     /locus_tag="PLI55_08165"
     CDS             2452..3426
                     /locus_tag="PLI55_08165"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPB00848.1"
                     /translation="MNRRIKVAFLGIMIGTNLFAFEKVGVTSFQFLKVIPDARSTAMG
                     EAYTSIANGADGLYWNPAAMMRSHALSINISQVDYIFDTKHFGTSISYTFNRFALGFT
                     FSGVDYGEIEVTDVNHLGFLPDGTYNPGVTGETIQPGAFVVGVGGAQRLTNKFTYGIN
                     MKYVQEDMEVAQKSLLMWDMGILFETGFRTVTLSATIRNFGPQVEYFDYSYPLPQTMN
                     IGISSYLIGDNSPLLLKNDLLKLLVAFDLVQPRDYDQQYNIGLEYTFRNIMILRAGYK
                     INYDTEGLTLGTGLRLKGLGIDYSYCSYGKYLSAVHRFSLSFNREIFD"
     gene            3440..4951
                     /locus_tag="PLI55_08170"
     CDS             3440..4951
                     /locus_tag="PLI55_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00849.1"
                     /translation="MKKEIRMLVIISLYILVSYNFINAQNRGNNIFFQGVENIAESNA
                     SVLGLGGAYTSIYGTVNVLSYNTACLAGINNIQVSISGGSVTRKWTENQEYYPNRLFV
                     TLPFYLQGLYIPDPANNGKRDSDIFYQGLEDSLYRVAFPDTGLESFSDEAADWIKKGK
                     AAGLTNIAIAYPVKIANKNFVLAASWNKKLDFFDYDRNETYLDPHLGYTEYDMPQMVN
                     GTDSVQVNWYIFDRYRSGSLQEYRGALAVDLFKNLKLGIGFAYLYGKTSDNQILDKVG
                     WFRLFDINRYSFSYDTLKMTTSGTSKFSGYKADLGLLLKLERLNLGLNLKMPYTIIKK
                     SDYDIAIADTDSENVITQKSTDKLKVPFCYTVGVSLYPTDKFMISLDYDLNLYSQAKW
                     EIASNDTIHQEWVDQKIIKFGLQYSPFKFLDVRAGYSLIPQVWVPDGSAGRNRGPEAK
                     ALTIGAGFKLDNYGTIDIAWEHRSLKYFDQYFSNTNYCKDITNNVLISYRYEF"
     gene            4973..6691
                     /locus_tag="PLI55_08175"
     CDS             4973..6691
                     /locus_tag="PLI55_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB00850.1"
                     /translation="MRKLATVLIITLLFIAITYAQMPSKIEVSADFMAQYFNNDFSKV
                     LDYTAQGVDTIVLTTSGHVYTTTDTIPMVIENPVVIVAQEGLAEKPIFTHPNTGFKSG
                     EPSSSMEIFRICNHVEFQGIAFRGDVAETEGCKYGLRYGDWTVPATGREIKGRMGTRM
                     IFKNCDFIGFHSLKDQAAQGNALYYLKPTDTNLDHLKNTKVVFENCMFKDIGDEAIRI
                     TENEKYGGPNGVVACDSLVVKNCTFDDIDAECIRIYADLDTSQAGSTFVDGTIFVDHV
                     TVVNSSPRFIYAKNFRNAMVQNVLIAYGREPGISRPDRGDYAIQIQLSGSSVAYVDTF
                     NLVFTLEYNPRIGCTKGGYVDETTVYGYDPMFVDYENGDYTLASNSPLYWLSSDGTAI
                     GDLRWATKTPTIAPLIVKVEGSGSVLFEPENTGFYNLNTVVTLTAVPEEGWEFKGWGG
                     DLTGSDNPANVTIDGAKNITATFELSSGIKEESTPKEYRLAQNFPNPFNPTTTIEFDL
                     KQAGWTTLSIYNILGQEVTVLVNKPMNAGWYTINFNASHLSSGIYYYRLKSGDFNSLK
                     KMLLIK"
     gene            6975..>9375
                     /locus_tag="PLI55_08180"
     CDS             6975..>9375
                     /locus_tag="PLI55_08180"
                     /inference="COORDINATES: protein motif:HMM:NF028470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5123 domain-containing protein"
                     /protein_id="HPB00851.1"
                     /translation="MFKLTGKNAAYVFKGLEIDGTNGTGTALAKYFLRIDNADSTGKM
                     FVSVRDCYLHDFADKFIKPYANSGIDELIVDNSVFSGGATEGIVLYSGTSSDPAVHLR
                     KASITNSTFYKIEREAIKGQTFDSTLVLIDRCTFYDCGNVEAKSILYFRNMEDVEVKN
                     SIFANNHNPDAGEEFADFASSVSLFHHNVAWDIVNIEVGSATVSDTLRADPQFADASN
                     GNFTIGNTTLLTFADDGGSVGDPRWVPEPTVIQIAAGTDVLSAAIASVEDGGTIELTT
                     SGGIYSESAQISSISKNITIRAAEGLANKPIIRVNTTDYILKLTGVNSRYVFKGLEID
                     GSNGTGSSVSKYFLRIDNLDPAGNMEVIVDDCVIHHFTDKFIKPYGNTGMDSLVVRNS
                     ILYNGGSEGIVFYSGSSGDPAAIIAYGEVSNTTMYNITRECIKGDTYTDGKIVIDHLT
                     AYNCGGGTKGMIYFDDWLDVTVKNSIFVKNTFASNFARFESDANIVKNCVIWDVASYK
                     TEGTTSVTDTLHVDPQFADAPNGDFTIGNPILFTFADDGSPVGDPRWVSLEGKYTLNV
                     IIEGSGQVILDPPGRIYDAGTVVKLTAVADSYWKFTGWSDNISVFPPNNPVAQVTMNQ
                     NMEVTAYFEPTINKYEVTVSSIGLGHVEIEEYSIFELPGYYEGDSLVLKPIADTTSWE
                     FAYWVNTAGDSIGNANPLSYIVHADTAFTALFRSKLPQVSLTVNISGLGSVLIDPQPV
                     EGFDTYDQGTEITLVAEPELGWEFQDWSGGITGTEATVVLTLNSDMTITATFIEKAVP
                     NG"
BASE COUNT         2782 a         1598 c         2103 g         2892 t
ORIGIN      
        1 acaatactcg ccatttgtaa ccagtcaaga tatttacctt ttgactaatg agccaagaaa
       61 tgtgcgagtg ggattaattt ttaagttcta aaattggatt aataaaatac tatgaagaat
      121 ctcaaaaaac tatttttcat ttttctgata acaggcactt atatcaactt gtatagccaa
      181 gtaagagagt accgtatcca taaccggggt atacttcatg aaacagtatt taatactggt
      241 gagatcggtc gggcttggca aacaggggag gctggtaatg aaaccagtgt ccccttaatg
      301 gagtggccat ctaactccag aacgatttta gatggaattg aatatagcgg acaacataac
      361 attattggcg ccggtgttta tatcggtgcc aacaaagagg ggttaccagg taagtccaat
      421 cgtttatttg ccttatgtgg tgccgtaggg gccagtgatc cggaagttgc aattgggcgc
      481 tggagctttc ccttatatat ggaagagatt gaaaacttcc cgttaaattc agatggttcg
      541 ctgaatgaaa attataatcc aaatgaagct gaagaaatta tcagcgctgg ttggtccaca
      601 ccaacgggaa taagtgttta ccgtacatct cgggcttgga gctatcccga ttacgatgat
      661 ttaatcatct atgaatacga atttgaatat actggcgata cagatggtga cccctccacg
      721 attgaaatga atgataaatt aaaagatgta atgatttgct ttaattatgg tttatacccg
      781 tcgatgtacg gagctcaaag agtttatctt gaatggaaat atgaaggcgg tctatatcgg
      841 ggagatgtta ataatttttg ggatgctgat tattggctga cctggaatat gaatcttcgc
      901 aataatatta ctgatgaaaa tggcatgggg aagcccgaac caaatcctga ttattttctc
      961 caatttgccc gaagtggtga acatggtgga ggactaaatt caccccaagc tccaggctat
     1021 tgtgttcttt tttacgacac tactcacctg gcagtcgtaa ttcccccaga aatggataca
     1081 ttgggtttaa atgaatcgga agcccaagcg attcttcgtt cgactacagt tacatctctt
     1141 agccaatatt ctggaattga tgtatataca gagacaacag gtgggtataa atggtactat
     1201 gagttagata aaagttttca tattcgacag ccatgggata ataaagtcag tacgggcaac
     1261 accaacagtg aaaaaataat gtatgaaaag gagccgttta atccatcaac acgctggtct
     1321 ggggtttata gccctggcag tgctacctgg ccggaagtaa aaagtgatga ttggtatggt
     1381 cgagcggcct atccttatcg tcaaagtgct gatgctggaa tgcgcttgca tgccttcggt
     1441 ccatatacct taaaacctgg tgataaaatc cggctggctt atgccgaagt aattgggtat
     1501 ggtgctcaag agggtaaaag agttgaagga ggtcagaccg caacacaatg ggcaaaaata
     1561 ccgtcgctaa atcggaaagt tacctacaag gatagtaaag gtaatgagaa ggttttaact
     1621 gagcattact tggatgattt tggttatcct gattatgtta attctaatat aattactgtt
     1681 catgatgtag cccggaaagc tttcgaagcc tatctcggga atgaacccgt ggtacccgtt
     1741 cagccagaga aacatccttc acgaggaaca tattcagtac ctacaccacc acctgccccg
     1801 gttatttcaa taaagaatta cggtttggct gaagtaaaaa taacttgggg cagagctcag
     1861 gaacaattta cccacccacg tttaactggt gatttggcaa agtatatggt tcaacgcgct
     1921 gtctccggga tgggcccgtg gattacactt gatactattg atgttggcga taatgaatat
     1981 ctcaatgaga atgatgaata tgagtacatt gattccgacc cgacctacaa tgttggtgaa
     2041 tttcgttact atgctgttgt atctgttgat gagcatggta ataaaagcgg taaaactaat
     2101 atgacctgtt ttcagaaaaa tattccggcg gttgaaaaaa tagataaagt ctatgtcgtt
     2161 ccaaatccgt ttatttgcaa atcaggcttt accactgaag gtgacgagag gatgatcggt
     2221 ttttatggat tgcctgatgt ttgtactatt cgagtgtttt catattcggg tcagttgatt
     2281 gatacaatcg agcataaaaa ttccgcttat tcccatgaaa aaatgcttgt tactcgaaat
     2341 aatcaggata tggcatcagg gatttacttt tatgtggtaa ctacacctga gggagaaaaa
     2401 tcattcggaa aatttattat tcttaaataa tagtctgatg gggatgaaga tatgaatcgt
     2461 agaataaaag tggcattttt aggtataatg ataggcacta atctttttgc ttttgaaaaa
     2521 gttggggtaa cttccttcca atttctaaag gtaatcccgg atgcgcgttc aacggcgatg
     2581 ggagaagctt acacatctat tgctaatggt gccgacggat tatattggaa tccagcggct
     2641 atgatgcgaa gtcatgcttt atcaataaat atttcccagg tagattatat ctttgatact
     2701 aaacattttg ggacgtcaat cagctatacc ttcaatcggt ttgcactcgg attcaccttt
     2761 tcaggagttg attatggcga aattgaggta actgatgtaa accatctcgg ttttttacca
     2821 gatggtacat ataaccctgg cgtgactggt gaaacgatac agccaggtgc ctttgtggtt
     2881 ggtgtaggcg gtgcccaacg tttgactaat aaatttactt acggtattaa tatgaaatat
     2941 gttcaggaag atatggaggt tgctcaaaaa tcccttttaa tgtgggatat gggaattcta
     3001 tttgaaactg gttttagaac ggttactcta tcagcaacta tccgcaattt tggtccacag
     3061 gttgaatatt ttgattattc ctatcctttg cctcaaacta tgaatatcgg aatttcatcc
     3121 tatttaattg gcgataattc accattgtta ttaaagaacg atttactcaa attattagtg
     3181 gcatttgact tggttcagcc acgcgattat gatcagcagt ataatattgg tttggaatat
     3241 accttcagga atattatgat actgcgagca gggtataaaa ttaattatga tactgaaggt
     3301 cttactttgg gcacagggtt aagactgaaa ggattgggga ttgattattc ctattgttcc
     3361 tatgggaaat atttatccgc tgttcatcgt ttttctctgt catttaaccg tgagatattc
     3421 gattaggaga aaatattcaa tgaaaaaaga aattcggatg ctggttatta tctcattata
     3481 tatattggtg tcatacaatt ttattaatgc tcagaaccgc ggcaataata tattctttca
     3541 aggagtggaa aatattgccg agtctaatgc atccgtttta ggtttaggtg gagcatatac
     3601 ttccatctac ggtacggtta atgttctatc ctataatacc gcctgcttag caggaattaa
     3661 caatatccaa gtatctattt caggtggttc agttactcgt aaatggaccg agaatcaaga
     3721 atactatccg aaccgcttat ttgtcaccct gccattttat ctccagggtc tttatatccc
     3781 ggatccggct aataacggta aaagggattc tgatatattt tatcagggct tagaagattc
     3841 tttgtacagg gttgcttttc cggatacggg attggaatct ttttcggacg aggcggctga
     3901 ttggataaag aagggaaaag ccgcaggact gaccaatatt gcgattgcct atcctgtaaa
     3961 aattgccaat aagaattttg tgttagcggc atcttggaat aaaaaattgg atttttttga
     4021 ttatgatcgg aatgaaacct acctcgaccc ccatttaggt tatactgaat atgatatgcc
     4081 ccaaatggtt aacggcactg attcagtcca ggttaattgg tacattttcg atcgctaccg
     4141 gagcggcagt cttcaagaat atcgaggcgc tctagctgtt gatttgttta aaaatctaaa
     4201 actcggcatt ggatttgcct atttatatgg taaaacctct gacaaccaga tcttagataa
     4261 ggtaggttgg ttcagacttt ttgatataaa tagatattca ttttcgtatg atacgttaaa
     4321 aatgacgact tcgggaactt caaaattctc cggttataaa gccgacttgg gcttattatt
     4381 gaaattagag agattaaatc tcggactgaa tcttaaaatg ccctacacga ttattaaaaa
     4441 aagcgattac gatattgcca ttgctgatac ggattcagaa aatgttatta cccaaaaaag
     4501 tactgataaa ttaaaagtgc ctttttgtta tacggttggg gttagcttgt atcctaccga
     4561 taaatttatg atttcgctgg actatgatct aaatctctat agtcaggcga agtgggaaat
     4621 cgctagtaat gatactattc atcaagaatg ggtcgatcag aaaataatta aatttggttt
     4681 acaatatagc ccattcaaat tcctggatgt tcgggctgga tatagcttaa tacctcaagt
     4741 ttgggttccg gatggttctg caggtagaaa tcgcggtcca gaagccaaag ccttgacaat
     4801 tggagccgga tttaaattag ataattatgg tactattgat atcgcttggg agcatcgttc
     4861 attgaaatat tttgatcaat actttagtaa tactaattat tgtaaggaca taacaaacaa
     4921 tgtcttaata tcatataggt atgaatttta atttaaaaga aaggagcaaa ttatgagaaa
     4981 attggctaca gtattaatca ttacgttact gttcatagca attacttatg ctcagatgcc
     5041 ttcgaaaatc gaagtgtccg cggattttat ggctcagtat ttcaataatg attttagcaa
     5101 agttttggac tatactgctc agggagtcga tacaattgtt ctgacgacca gtggacatgt
     5161 ttataccacg accgatacta ttcctatggt tatagaaaat ccggtcgtta ttgtggctca
     5221 ggaaggatta gcagaaaagc cgatatttac ccatccaaat accggattca aaagtggtga
     5281 accaagtagt tcaatggaaa tatttagaat ttgtaatcac gtcgaattcc agggtattgc
     5341 tttcagggga gatgttgctg agacggaagg ttgtaaatat ggcttgcgct atggtgattg
     5401 gacggttccg gcaactggtc gtgagattaa aggcagaatg ggtacgcgta tgattttcaa
     5461 aaattgtgat tttattggtt ttcatagttt gaaagatcag gcagctcagg gtaatgcatt
     5521 atactactta aaaccgactg atactaatct tgatcatcta aagaatacca aagtagtttt
     5581 tgaaaactgt atgtttaaag atatcggcga tgaggctatt cggataactg aaaatgaaaa
     5641 atatggtggt ccaaatggag ttgtggcttg tgattctctt gtggtaaaaa attgtacttt
     5701 tgatgacatt gacgccgaat gtatccggat atatgctgat cttgacacca gtcaggccgg
     5761 gtcaaccttt gtagatggga ctattttcgt tgatcatgtc acggtagtaa atagttctcc
     5821 gcggtttatc tacgcgaaaa attttcggaa tgcgatggtt cagaatgttt taattgcata
     5881 tgggagagaa ccaggaattt ctcgcccaga ccgaggtgat tacgctattc agattcagtt
     5941 gagtggttct tcggtagctt acgtggatac atttaatttg gtgtttacat tggaatataa
     6001 tccaagaata ggctgtacga aaggtggcta tgttgatgaa acaactgttt acggatatga
     6061 tccgatgttc gttgactatg aaaatggtga ttatactctg gcctccaact caccactcta
     6121 ctggctatca tcggacggta ccgccatagg tgatttgcga tgggcaacta aaactcctac
     6181 gattgcgccg ctgatagtaa aagttgaggg ttctggtagt gttctttttg aacccgaaaa
     6241 cactggattt tacaacctga atacagtggt gacacttacc gctgttcctg aagaaggctg
     6301 ggagtttaag ggctggggtg gtgatttaac cggttcggat aatccggcaa atgtaacaat
     6361 agacggagcc aaaaatatca cggcaacctt tgagttatct tctgggatta aggaagaaag
     6421 tacacccaag gaatacagac ttgcccagaa tttccccaat ccttttaatc caaccacgac
     6481 tattgaattt gatttgaaac aagccggttg gacaacactg agtatttata atatactagg
     6541 tcaggaagtg acggttctgg taaacaaacc aatgaatgca ggatggtaca cgatcaattt
     6601 taatgcctca catctcagtt caggaatata ttattatcgc ttgaaatccg gtgatttcaa
     6661 ttcattaaag aaaatgcttt taattaaata acaattagga ggaaaacatg aagttcaaaa
     6721 atttagtgct tattctttcg atttttttgt tgggtagtat aacgtatgct tcagtcattc
     6781 aagtttctga gggaactggt gtactcagcg cggcgatatc atccgcggcc gatggtgatg
     6841 tgatcgagct gataagtgat ggtggaatct atgccgagag tgcaaaattc ccttcaatta
     6901 gtaagagcat tagcatcgtg gcggctgaag gacttgttca taaacccgtt attcggacac
     6961 ccgaggcgga ttatatgttc aaattgactg gcaaaaacgc cgcttatgtt tttaaaggtt
     7021 tagagataga tgggacaaat gggactggga ctgctttagc taaatatttt ctgcgtatcg
     7081 ataatgcaga ttcgaccgga aagatgtttg tcagtgttcg agattgttac ttacacgatt
     7141 ttgccgataa atttattaaa ccttatgcaa atagtggaat cgatgaattg atcgtcgata
     7201 attcagtttt tagcggtgga gctacagagg gtatagtact atactcaggc acttcgagcg
     7261 atccggcagt tcatctcaga aaagcatcca taaccaattc cacattttat aaaatagaaa
     7321 gggaagcaat taagggtcag acgtttgatt ctactttggt tttaattgat cggtgcactt
     7381 tctatgactg tggtaatgtg gaagccaaat caatcctcta ttttcgtaat atggaagatg
     7441 tagaggtgaa gaacagtatt ttcgctaata atcataatcc agacgctggg gaagaatttg
     7501 cggattttgc ctcaagtgtt tcacttttcc atcataatgt tgcctgggat atagtaaata
     7561 tagaggttgg tagtgctacc gttagtgata cccttcgtgc tgatcctcaa tttgcggatg
     7621 catcgaatgg gaattttaca attggtaata ccaccttgtt aacctttgcc gatgatggtg
     7681 gttctgttgg agaccctcgc tgggttcctg agccgaccgt tattcaaatt gcggcaggaa
     7741 ctgatgttct cagtgcagca attgccagcg ttgaagatgg aggtactata gagctaacaa
     7801 cctccggtgg tatttattcg gagagtgcac aaatttcttc gattagtaaa aatataacca
     7861 tccgcgcggc tgaaggatta gcaaataaac ctataatcag agttaataca accgattata
     7921 tccttaaact cacgggtgtt aattcaagat atgtttttaa aggtctagaa attgatggtt
     7981 caaacggtac gggttcgtcg gtatcgaagt actttttgcg aattgataat ttagaccctg
     8041 ccggtaatat ggaagttatt gttgatgact gcgttatcca tcattttact gataaattta
     8101 ttaaaccgta tggaaataca gggatggatt cattagtagt aagaaatagc atcttatata
     8161 atggtggttc tgagggtatt gtattctatt ccgggtcttc cggtgacccg gcggcaatca
     8221 ttgcttacgg tgaagtgtcc aatactacga tgtacaatat tacccgtgaa tgtattaaag
     8281 gcgatactta tacagatggc aaaatagtaa ttgaccatct aacggcttac aattgtggtg
     8341 gcggaacgaa gggtatgatc tattttgatg attggctgga tgttactgtc aagaatagta
     8401 tttttgttaa aaatacattt gcgagtaatt tcgctcgttt tgaatcagac gccaacatcg
     8461 ttaaaaattg tgttatttgg gatgttgcca gttataaaac agagggtacg acttcggtta
     8521 cggatacctt gcatgtagat ccacagtttg ccgatgctcc aaatggcgat tttaccattg
     8581 gcaatcctat cctatttaca tttgccgatg atggtagccc agtaggagat ccacgttggg
     8641 tctcgttgga ggggaaatat actctcaacg taataattga agggagcggt caggtaatat
     8701 tagatcctcc cgggagaatt tatgatgccg gtactgttgt aaaattgact gctgttgcgg
     8761 atagttattg gaaatttacg ggctggtcag ataatatatc ggtttttccg ccaaataatc
     8821 cggtagcaca ggttacaatg aatcaaaata tggaagtgac tgcttatttt gaaccaacaa
     8881 tcaataagta tgaagttact gtcagttcaa tagggctcgg gcatgttgag attgaagaat
     8941 actctatttt cgaattaccg ggttattatg aaggagattc actggtttta aaacctattg
     9001 cggatactac gagttgggaa tttgcctatt gggtcaatac cgcgggtgat agcatcggga
     9061 atgcgaatcc gctatcctat atcgtgcatg ccgatacagc ttttacggct ttgtttcgta
     9121 gtaagttgcc tcaagtttcc ttgacggtca atatctcagg tcttgggagc gtattgattg
     9181 atcctcagcc tgtggaagga tttgatactt acgatcaagg tacggaaata actttggtgg
     9241 cagaaccgga gcttggatgg gaatttcagg actggagtgg tggtattacc ggtactgaag
     9301 ccactgtagt cttgacactt aacagtgata tgacgataac agctacattt attgaaaaag
     9361 ctgtgccaaa tggag
//
LOCUS       DAOOMU010000086         9227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_8741_length_9227_cov_19.111208, whole genome shotgun sequence.
ACCESSION   DAOOMU010000086 DAOOMU010000000
VERSION     DAOOMU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 9227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9227
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8741_length_9227_cov_19.111208"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..804
                     /locus_tag="PLI55_08185"
     CDS             244..804
                     /locus_tag="PLI55_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPB00852.1"
                     /translation="MNRAVFLDRDGTLNYDSRDYIKNVAEFQLFPITVAALSKLYSAG
                     FKLIVITNQACVAKGLTTVAAVEEIHQLLRKKLSEAGIELAGIYYCPHDPEAGCDCRK
                     PRIGNILLAARELEISLSDSYFVGDSHRDVEAGRAAGCRTILVTTGVRQSSYADIAEW
                     QVQPDYVVPDLLTAAEMILSLERKMN"
     gene            804..1793
                     /locus_tag="PLI55_08190"
     CDS             804..1793
                     /locus_tag="PLI55_08190"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPB00853.1"
                     /translation="MKIAVLMGGVSSEREVSLSSGRNIVAALRENGHEVLALDTVLPI
                     EQLSQDIQPTSEHLQKGEENLISLLSNATVRAVDFVFNALHGGSGENGAVQSVLEIMG
                     YKYNGSDHEGCAIAMDKIVSKMIFEGIGIRTPEWISFDLKVGYDPVAIQEEILRKIEP
                     PLVIKPAHEGSTVGLSIVKEVAGIQPAIATAFKYNRALLVERYIPGREITIAILGEMA
                     LPLVEIRPKHGIYDYECKYTSGMSEYIVPALVDEKIVKQIQEQALVAFKALRCSGYGR
                     FDLRLTDEGNAFFLEFNTLPGMTATSLVPKAAKAIGMNFNALLEKIIEIGRDR"
     gene            1790..2386
                     /locus_tag="PLI55_08195"
     CDS             1790..2386
                     /locus_tag="PLI55_08195"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPB00854.1"
                     /translation="MSDKKNVDPELIKSNSNSLDTKGKHWYAVYTRPRHEKKVNGLLQ
                     EQQIESYLPLVSTKRQWGNRWAQVEEPLIRSYVFVHIRLNQTLYVLETYGVVRFVMFA
                     RELAVVPDFQIEALKKSLESGFTLSPINYMQVGQLVEVTEGPLKGVIGKIQQIGKNER
                     FVLTLDAVQAAYTIQIDPQWLRPLSPEKKKIFTLPLGF"
     gene            2388..3785
                     /gene="cysS"
                     /locus_tag="PLI55_08200"
     CDS             2388..3785
                     /gene="cysS"
                     /locus_tag="PLI55_08200"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPB00855.1"
                     /translation="MSIYFYNTLSRQKEEFRPIEEGVVRLYTCGPTVYNFAHIGNYRA
                     FVFEDLLRRFLKFKGFRVIQVMNITDVDDKIITAANREGVSIREFTEPFRQAFMEDLT
                     TLGIEPAEYYPRATEHINEMVALIETLLKKGVAYRSEDGSIYFKVSEFPRYGQLANLN
                     VTEMRRGDRVANDSYEKEEIRDFALWKAWKPEDGAVFWETSLGKGRPGWHIECSAMSM
                     KYLGRHFDIHTGGVDNIFPHHENEIAQSEAATGEKFVNYWMHCEFLLVNDEKMSKSIG
                     NIIYLRDLVKKGYNPTAIRLTLLGTHYRQKLNFSFEKLEESGKIITRLRDFKKSLEGE
                     FPMGNAGAMEIVEKGEADFEAALDDDLNISNALAAVFKLLHEINLYRQKTPLTQADVE
                     LIRQSLKRIDSVLNILPEPLDMLSEEERKLIDARNQARRERNWAEADRLRNLLLERGI
                     ILEDTPKGTTWKRKI"
     gene            complement(3986..4969)
                     /gene="rfaE1"
                     /locus_tag="PLI55_08205"
     CDS             complement(3986..4969)
                     /gene="rfaE1"
                     /locus_tag="PLI55_08205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HPB00856.1"
                     /translation="MLETLPTDLNNLDKIFQEIQNLHILVVGDLILDQYIWGKVERIS
                     PEAPVPIITVSRKENRLGGAGNVAANLSALECPVTLAGVCASDQAGRDLQTLCHQKKI
                     NSIIATLPERRTSCKARIIAQQQQLLRIDEETVEPVDQKETEQLLKNITAFSDRFDAV
                     ILSDYAKGLVTEDFIQSILEIFKDKPVLVDPKGFNYQKYRGATALKPNFREFCMAVRH
                     PELTLTKIEPLARQLIDELNLKGLIVTLGEQGVFVLDTDQKAALIPTKAREVFDVSGA
                     GDTFIAVFTAVLMVTNDWKIAAVVANLASGIAVGKIGTATVTTAEILAEDY"
     gene            complement(4975..5934)
                     /locus_tag="PLI55_08210"
     CDS             complement(4975..5934)
                     /locus_tag="PLI55_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPB00857.1"
                     /translation="MSILVTGSAGFIGFHLAHRLLQEGIEVVGYDNVNDYYDPSLKEA
                     RLRILNDFDKFKFYRADLCDFATLQKVFSDQTIEKVVHLAAQAGVRYSLINPFAYQKS
                     NLEGFLNIIELSKRAKVKNFIYASSSSVYGNNRHLPFSVQDNVDRPISLYAATKKSNE
                     LIAHVYAHLYQLPCTGLRFFTVYGPFGRPDMALFIFTKNILEGKPIDVYNFGQMKRDF
                     TYIDDIVAGLRSAIDKPFPYEIFNLGNHRSENLMDFIHLIEEYSGRKAQINFQPIQPG
                     DVPETYADISTATEKLGFVPRTQIREGIKLFLDWYFEYYHIKK"
     assembly_gap    6557..6656
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6657..8084
                     /locus_tag="PLI55_08215"
     CDS             <6657..8084
                     /locus_tag="PLI55_08215"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB00858.1"
                     /translation="YQVTAMNGYSVNKPKLDVVGDQAMITFATNFNFYSFPLTGPTSP
                     IENPVKPDMNAWGPNHVDIAHANGQVYIVYTDYKRNDQNGLYEVRYITKTIGSPEWTI
                     PTILDTVSIGYSIMMTYTNQDLQVGANGAYLLWRNQLDDNSIYFSKSYGSLFSQKRKV
                     LGDSLSLIKDYSFTVIPVEGTDHLFMIYAQDSSLFFVSSSDEGNTFSEPTKIDSLEGF
                     WSNFYDLQIVGLSNGTLYAKGQFWQFVQHWDETEPPGEDEPPMDMYGTRLYRSTDFGA
                     HWSIVNTLPEGNSQIALTPGGTIVLTQYPDVNLYVKTSTDAISWSDSVRINPNENTAT
                     GWSDIGFDVWIIDETTMAVAWIDTTTGYDEIWYRTLTIPTPPLVSIEKDGINTPQKAI
                     LVQNYPNPFNPVTKFSYQLPSESFVELSIFDINGRLVDKIVNQFQPAGYYSVQWNAGN
                     YSTGVYLYKFRADNFQQVGKCLLLK"
     gene            8581..8653
                     /locus_tag="PLI55_08220"
     tRNA            8581..8653
                     /locus_tag="PLI55_08220"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8614..8616,aa:Thr,seq:tgt)
     gene            8814..8897
                     /locus_tag="PLI55_08225"
     tRNA            8814..8897
                     /locus_tag="PLI55_08225"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8848..8850,aa:Tyr,seq:gta)
     gene            8906..8976
                     /locus_tag="PLI55_08230"
     tRNA            8906..8976
                     /locus_tag="PLI55_08230"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8938..8940,aa:Gly,seq:tcc)
     gene            9020..9092
                     /locus_tag="PLI55_08235"
     tRNA            9020..9092
                     /locus_tag="PLI55_08235"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9053..9055,aa:Thr,seq:ggt)
BASE COUNT         2670 a         1767 c         2071 g         2619 t
ORIGIN      
        1 ataaataaaa ccttctgcag gtttttttat gccctgccct attattttac gcctccggat
       61 tgtaatttat gtctggattt ttatggagaa atgaaggtga gtgtcaatag cgatgtttga
      121 ggatgtagaa ggcgaggaca gccaagtatg ccaaagtcag ataatagttt attttatgtt
      181 atttaagttg acaaacttat ccacagcatc ggcgctaaat ttaggacagg taattttatt
      241 aaagtgaata gagcagtttt tttagatcgg gacggcaccc tgaattatga tagccgcgat
      301 tatatcaaga acgttgcgga atttcagctt tttccaatta cggtcgcggc tttaagtaag
      361 ttatattctg ctggattcaa attaatcgta attaccaacc aggcttgcgt cgctaaaggc
      421 ttgacaacag ttgccgccgt cgaggaaatt caccaactat tgcggaaaaa attaagcgaa
      481 gccggcattg agttggctgg tatttattat tgtccgcatg atccggaagc cggttgtgat
      541 tgtcgcaaac cacgtattgg taatattctt cttgccgccc gtgaacttga gattagtcta
      601 tcagattcat actttgtggg ggattcacat cgagatgtcg aggcgggacg cgccgccggt
      661 tgtcgaacta ttttagtcac aactggtgtt cgtcaatcca gttatgccga tattgcggaa
      721 tggcaagtcc aaccggatta tgtggttccc gatttactga ctgcggctga gatgatttta
      781 agtttagaaa ggaaaatgaa ttaatgaaaa tcgccgtcct gatgggtggt gtttcgtccg
      841 aacgggaggt atcgctctcc agcggccgca atatcgtggc ggctttacgg gaaaatgggc
      901 atgaggtgct ggcgctcgat acagttttac cgattgagca attaagtcag gacattcaac
      961 caaccagcga gcatctgcag aaaggcgaag aaaacctcat tagtttatta agcaatgcga
     1021 ccgtccgcgc cgtggatttc gtttttaatg ccctgcatgg cggcagtggc gaaaacgggg
     1081 cggttcaaag cgtactcgaa attatgggtt acaaatataa cggcagtgac catgaaggtt
     1141 gtgcgattgc tatggataaa attgtttcga agatgatctt cgagggcata gggattagaa
     1201 cgccggaatg gatttctttc gatctaaaag tcgggtacga tccggtcgcg attcaagaag
     1261 aaatattacg aaaaattgaa ccgccgctgg tcatcaagcc cgcccatgaa ggctcaaccg
     1321 tcggcctctc cattgtaaaa gaggttgccg ggattcaacc ggctattgct accgccttta
     1381 aatacaaccg ggctttgttg gtcgagcgct atattcccgg gcgcgaaatt accatagcga
     1441 ttctcggtga aatggctttg ccgctggttg aaattcgtcc caagcatggc atttatgatt
     1501 atgaatgtaa atacacttcc ggtatgagtg agtatattgt gccggctttg gtggacgaaa
     1561 aaattgttaa gcagattcaa gaacaggcgt tagtggcttt caaagcttta cgttgttctg
     1621 gatacggccg ttttgatttg cgtctgactg atgaaggcaa tgcctttttc ctggaattta
     1681 ataccctgcc cggtatgacg gcgaccagtt tagtaccgaa agccgccaaa gctatcggta
     1741 tgaatttcaa tgctctactt gaaaaaatta tagaaatcgg tagggataga tgagcgataa
     1801 aaaaaatgtt gacccggagt tgattaaatc caattccaat agtttggata ccaaaggtaa
     1861 acattggtat gcagtctata cccgaccgcg acatgaaaaa aaagtcaatg gacttcttca
     1921 agagcaacag attgagagtt atttaccttt agtatccacg aagcgacaat ggggcaaccg
     1981 ctgggctcag gttgaagagc cattgattcg tagctacgtc tttgttcaca ttcgtctcaa
     2041 tcagacgctt tatgttctgg aaacctacgg cgtggtgcgc tttgtaatgt ttgcaagaga
     2101 gctggcggtc gtgcccgatt ttcagattga agctttgaaa aagagcctgg agagtggttt
     2161 tactctttca ccaattaatt atatgcaggt tggccaattg gttgaagtta ccgagggacc
     2221 attaaaaggc gtaatcggaa agattcagca aatcggcaaa aatgagcgat ttgttctgac
     2281 gctcgacgcg gtgcaagccg cttatacaat tcagattgat ccccagtggc ttcgtccgtt
     2341 aagtcctgag aagaagaaaa ttttcacgct accactcgga ttttaatatg agcatttatt
     2401 tttataatac cctgagtcgc caaaaggaag aattccgtcc gatagaggaa ggtgtggttc
     2461 gcctttatac ctgtgggcca acggtctata attttgctca tatcggtaat taccgggctt
     2521 tcgttttcga agatttattg cgacgattcc tgaaattcaa aggtttccgt gttattcaag
     2581 taatgaatat taccgatgtt gatgataaaa ttatcacagc ggcgaatcgc gagggcgttt
     2641 ccattcgcga atttaccgag ccctttcggc aggcgtttat ggaagaccta acgacgttgg
     2701 gaatcgaacc agctgaatat tatccgcgcg ctaccgaaca tatcaatgag atggtggcat
     2761 tgattgaaac tttgttgaaa aagggtgtcg cttatcgctc tgaggatggt tcgatttatt
     2821 tcaaagtttc cgaatttccg cgctatgggc agttagccaa tctgaatgtt acggaaatgc
     2881 ggcgtggcga tcgagtagcc aatgattcct acgagaaaga agaaattcgt gattttgcac
     2941 tctggaaagc ctggaaaccg gaagacggtg cggttttctg ggaaacgtcg ctcggcaagg
     3001 ggcgtcccgg ctggcatatc gaatgttccg cgatgagtat gaaatatctt ggacggcatt
     3061 tcgatattca taccggcggc gttgataata tttttcctca tcatgaaaat gaaattgccc
     3121 aaagtgaagc cgccaccggc gagaaattcg ttaactattg gatgcattgc gaatttttac
     3181 tggtaaatga tgaaaaaatg tcgaagtcaa tcggcaatat tatttacctg cgggatttgg
     3241 tcaaaaaagg atataatccg actgctatta ggttaacttt gctgggcacc cactatcggc
     3301 agaaattgaa tttcagtttc gagaaactcg aagagtccgg caagatcatt acgcgcttgc
     3361 gcgattttaa gaaatcgctg gaaggtgaat tcccaatggg aaatgccggt gctatggaaa
     3421 tcgtcgagaa aggtgaagcc gacttcgagg cggcgctcga tgatgatctg aatatctcca
     3481 atgccctggc ggctgttttt aagttattgc acgaaatcaa tttatatcgc caaaaaactc
     3541 cgctgaccca ggcggatgta gaattaattc gacaatctct aaaacgaatt gattccgttc
     3601 tgaacattct gcccgaaccg cttgacatgc tttctgaaga agagcggaag ttaattgatg
     3661 ccagaaatca agcccggcgg gaacgtaatt gggcggaagc cgatcgcttg cgaaaccttc
     3721 tcctcgagcg aggcattatt ctcgaagaca ctcccaaagg taccacctgg aaacgcaaaa
     3781 tctgagtttt caaaaggcca ataaattcaa aagaaactaa actctaaatc ccgagcgtcg
     3841 ggttgatttc ttgttggtta taaaagtttg ggaaattttt ctcccgtatt atgccgtaaa
     3901 tgttccgccg gaagaaaaac taatggaatc aattttaaaa ataacgaata tttcttagtg
     3961 ttctttgtgc cttagtggtg aatagctaat aatcttcagc gagaatttca gcggttgtga
     4021 cggtagccgt gccgattttc ccgacggcaa tcccggatgc taaattagct actacagctg
     4081 ctattttcca atcattagta accattagaa cggcggtgaa aacggcgata aaagtatcac
     4141 cggcaccgct gacatcgaag acttcgcgcg ccttagtggg aattagagcg gctttctggt
     4201 cagtatcgag tacgaaaacc ccttgttcgc ccagagtaac gatcaaaccc ttcaggttaa
     4261 gttcatcaat caattgtcga gcaagcggtt cgattttagt gagggtcaat tcgggatgac
     4321 gcaccgccat acaaaattca cggaaattgg gcttgagggc ggtcgcgcca cgatattttt
     4381 gatagttgaa acccttggga tcgaccagta ctggtttatc cttgaatatt tccagaatgg
     4441 attggataaa gtcttccgtg accagacctt tggcgtagtc ggaaagaatt acggcatcga
     4501 accgatcgga aaaagccgta atatttttaa gaagttgctc ggtctctttc tggtcaaccg
     4561 gctcgacggt ttcttcgtca atgcggagca attgttgctg ttgcgcgatg attcgcgctt
     4621 tgcaagatgt acgccgttcg ggcaatgtgg cgataattga gttaattttt ttctggtgac
     4681 ataaagtctg taaatcccgc ccggcttgat cggaagcgca cacaccagct aaagttaccg
     4741 gacattccaa agcgctcaag ttggcggcga cgttgccggc tccgccgagg cgattttctt
     4801 tacggctgac agttataata ggcacgggcg cttccggtga aatgcgttcg accttacccc
     4861 aaatatattg gtcgagaatt aggtcgccca ccaccagaat atgcaggttt tggatttcct
     4921 ggaaaatttt atccaggttg ttcaaatcgg taggtagtgt ttcaagcata ttttctactt
     4981 tttaatatga taatattcga aataccaatc gaggaataat ttgatgcctt ctcggatctg
     5041 cgtgcgcggc acgaagccga gtttttccgt agcggtggag atgtcggcat aggtttccgg
     5101 tacatcgccg ggttgaatcg gctgaaaatt tatctgagct tttcgccccg agtattcttc
     5161 gattaaatga atgaaatcca tcaagttttc actgcgatga tttccgagat taaaaatttc
     5221 atacggaaaa ggtttgtcaa ttgctgacct caaaccagcg acaatatcat caatataagt
     5281 aaaatcgcgc ttcatctgac caaaattata gacgtcaatc ggtttaccct caagaatatt
     5341 tttagtgaaa ataaacagcg ccatatccgg tcgcccaaaa ggaccgtaaa ccgtaaagaa
     5401 acgcaagccg gtgcaaggca gttgataaag atgagcataa acatgtgcga ttaattcatt
     5461 ggatttttta gtggcggcgt agagcgaaat tgggcgatca acattgtctt ggacggagaa
     5521 cggcaggtgg cgattattgc cgtaaacgct ggaactggaa gcataaatga aattcttgac
     5581 cttagcgcgc tttgataatt cgatgatgtt caaaaaacct tccagattcg atttctggta
     5641 agcgaaaggg ttaatcagcg agtagcgtac cccagcttgg gcggcaagat ggaccacttt
     5701 ttcgatcgtt tgatcgctaa agactttttg aagggtggcg aaatcgcaca gatcggcacg
     5761 ataaaattta aatttgtcaa agtcattcaa aattcgcaga cgggcttcct tcaatgacgg
     5821 atcgtaataa tcattgacat tatcatatcc gacgacttct atgccctcct gcagtagccg
     5881 atgagctaaa tgaaaaccga taaatccggc acttccagtt acgagaatac tcacttaatc
     5941 acacctaaaa ataaacgcaa aagattaaac caattatgtg agaaattcaa atgatttgtt
     6001 catacaaaaa aagtaggaat gggtgagcgc ttgaacaaat ttggtaacat ttgtcccaaa
     6061 acagatgtca agtgagtagt aattgttatc gggtgtgaat ttatgattgc taacataacg
     6121 agttgatttc ttggagattg ggtggttttt cggaattaaa tacagaaaat tcaccaatat
     6181 ttcccaaaaa tagctttaaa ataagccgaa gttttattaa aatattctac attagatcga
     6241 tttaataaac tacactttta tacttttaag agcagctttt tacttatgat tgaactttta
     6301 acatcaatat attataagag gttttgatga acacaaacca gcgtcaaaat actcgcttga
     6361 attaaaaatc cacaaaaatc cgttgagaat atgtaactta tttataagaa atatgctcta
     6421 atcagccgtg aaattaaact ttaacaaagg agttatttta ttatgaaaac tatcaaagtt
     6481 tccgtactta tgcttttgct actagcgtct tcgttgcttt ttgggcaagc ctatcaggtt
     6541 actgcaatga acggatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntatc
     6661 aggttactgc aatgaacgga tatagcgtta ataaacctaa attggatgta gtaggcgacc
     6721 aagcaatgat tacgtttgca accaatttta atttctacag tttcccgcta acaggtccca
     6781 catcacccat cgaaaatcca gttaaaccag atatgaatgc ttggggacca aaccatgttg
     6841 atatagccca tgctaatgga caggtgtata ttgtgtacac tgattacaaa aggaatgacc
     6901 agaatggcct ttatgaagtg cgatatatta ccaaaactat tgggagtccc gaatggacaa
     6961 tcccgacaat attagatacg gtttcaattg gatattcaat tatgatgact tacacgaacc
     7021 aggatctgca agttggagca aatggagctt atttactgtg gagaaatcag ctcgatgata
     7081 atagcattta tttttcgaaa tcgtacggct cccttttttc tcaaaagagg aaagtactcg
     7141 gagactctct ctcattgata aaagattact cgttcactgt gataccagtt gagggcactg
     7201 atcatctctt catgatttat gcgcaagatt cgtccctctt ttttgtatca tcttcagatg
     7261 aagggaatac tttttccgag cccaccaaga ttgacagtct cgaaggattc tggagtaact
     7321 tctatgatct tcagatagta ggcttatcga atggcactct ctatgcaaag gggcagtttt
     7381 ggcaatttgt acagcattgg gatgaaactg aaccgcccgg ggaggatgaa ccaccgatgg
     7441 atatgtatgg cacacggctg tatcgttcaa cggattttgg tgcgcattgg tctattgtta
     7501 atacgctccc cgaaggtaac tctcagattg cacttactcc cggtggaacg atcgtgctga
     7561 ctcaatatcc tgatgttaat ctgtatgtta aaacgagtac cgatgctatt tcatggtcag
     7621 attctgttcg tattaatcca aatgaaaata ctgctactgg atggtctgac attggtttcg
     7681 atgtgtggat tattgatgaa acaacgatgg cggttgcctg gattgatacc acaacaggat
     7741 atgatgagat ttggtaccgc actcttacaa ttccaactcc accacttgtc tcaatagaaa
     7801 aagatgggat taacactcct caaaaagcca ttctcgtaca gaattatccc aatccgttta
     7861 acccagttac taaattttcc tatcagctac cgagcgaatc ttttgttgaa ctatccatat
     7921 ttgatattaa cggtagattg gtagataaaa tagtaaatca gtttcaaccg gctggttatt
     7981 attcagttca atggaatgca ggaaattata gtacaggcgt ttacttgtac aaattccgag
     8041 ctgataattt tcaacaggtt gggaagtgtt tattattgaa ataaattcat aaaaatatgt
     8101 agctattggc tactcaacga ataccggaat ttactaaagt agagaagatt ttgcggatta
     8161 tagaattgga attgaaaagt acgaagacga actagattgt tcaggcgtta ataacaagat
     8221 ttcccaaaat attttatgtc tatccactat aaatagagta gcgatagttt ttatgatctt
     8281 attttagtag tggtttatcc ggttattccg ttcacaaatg ttttgcttgc tatgcggtgg
     8341 agtctaatct aaaactatta attccaaatt ggcatggaag actttgagta tagtagagat
     8401 tattcaccct cgagaacttt actttacagg gagtaactta cctacaaaaa ttggattttt
     8461 aaattggtga aagtaatagt cataaattaa aaattgagat tctgataaaa taaaaactta
     8521 ttgatttaat taataaattg attaaatttc gaggcttttt tgaaatttga tagacgacct
     8581 gccaccatag ctcagttggt agagcagctg atttgtaatc agcgggtcgt gggttcaaat
     8641 cctactggtg gctctaggat ttgatgatga tgctatttga aatggagatt tgacggatac
     8701 caaagcctga tttgcagagt aaatttggga gagagtaatt actttcgcta agtcaaacat
     8761 ttaaaatagc attttgcgaa tacaaattat ctttccaaaa cataaataaa agcgggtcgg
     8821 tggccgagtg gttaatggca gcagactgta aatctgccgg cgtaatgcct acggtggttc
     8881 gaacccatcc cggcccacag cttctgcggg tgtagcttag tggtaaagct ccagccttcc
     8941 aagctggcta cgagggtccg attcccttca cccgctcaac ttgcgattaa aagaaaagta
     9001 attgatttat cggaataatg cccacgtagc tcagtaggta gagcacatcc ttggtaagga
     9061 tggggtcacc agttcaactc tggtcgtggg ctcatacctt aatcggtttt tgggtaagtg
     9121 tcaaatcaaa ttgagaattt ttgtaaagga aaaataaaaa aaaggagcac caatggcaaa
     9181 gcaacaattt aagcggacga agccgcacct caacattggt acgattg
//
LOCUS       DAOOMU010000087         8726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_9442_length_8726_cov_17.509053, whole genome shotgun sequence.
ACCESSION   DAOOMU010000087 DAOOMU010000000
VERSION     DAOOMU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9442_length_8726_cov_17.509053"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..335
                     /locus_tag="PLI55_08240"
                     /pseudo
     CDS             36..335
                     /locus_tag="PLI55_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011841288.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(362..1615)
                     /locus_tag="PLI55_08245"
     CDS             complement(362..1615)
                     /locus_tag="PLI55_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HPB00859.1"
                     /translation="MKLKKYSLGIGDRFGHQAEAQLTALVKAAEKGVNITPVWNKSFR
                     EHNIIGSQPADVRHSAEKAVQALNWSKGYFVDADHINLKTVPHFIESSDFFTLDVADA
                     IGVPPNENDIREFLKFAEKYTQPFGIPGILQKIAVDHKSIEKIARKYLAAVLAAADTY
                     HYIQDNCQHDYVIELSVDETDKPQSPLELFFILSMAAWQELPLATIAPKFSGRFNKGV
                     DYVGDPAKFAVEFEQDVAVVKFAIKEFSLPDGLKLSVHSGSDKFSLYPHINSIITKTN
                     AGLHLKTAGTTWLEELIGLAAAGGEGLEIAREIYCRAYERFEELVKPYATVVDIDRTK
                     LPAPSVVNGWTSREYVSALRHNQSEKAYNPHLRQLLHVGYKIAAEMGERYLSALKKYA
                     EFIAPNVTENLYQRHIQPLFGVPVD"
     gene            complement(1717..2970)
                     /locus_tag="PLI55_08250"
     CDS             complement(1717..2970)
                     /locus_tag="PLI55_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017730486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 domain-containing protein"
                     /protein_id="HPB00860.1"
                     /translation="MSKPRLIIVAMLFLTSVFSCNNRSAKERPDLPLSFKIIVKNPTK
                     QVRKDAMVRINLADIKAKYPDFNPDAWVIFAGETEIPSQLNAEKTEILFVTDFAPSEK
                     KTFTIHYAKEGTITHNYPKRTQAELSHKVGGRFVNRIYEGGQFQNVKYLRVPPEHTDH
                     SLFIRYEGPGWESDKVGYRFYLDWRNAIDVFGKKVPDMVLQEIGQDGFESYHSMCPWG
                     MDVLKVGESLGLGSLGMWYEGKAERIAVTDSIICEILQDGILESRIRTRYYGWQIGGQ
                     KYDLVSELSIAAGSRLTRHDIQINDEPPNLCTGIVKMENVSLLSSTDQNRDWMYFATY
                     GKQSLANDKLGLAVLFRRQDLIQITDDENSYVVILKPTAGKLTYYFLAAWEQEPDGIK
                     TESQFADYLNETVAMLDNDLIITIK"
     gene            complement(3018..4280)
                     /locus_tag="PLI55_08255"
     CDS             complement(3018..4280)
                     /locus_tag="PLI55_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HPB00861.1"
                     /translation="MRKIALLTSLLICLAQIGTASEEFDNLMIVDQSGAGQFTSITAA
                     LAALPDMAYQRVVIFIRNGIYEEKIRLDQNYVTLRGENRDSTIIRFCQKRTDWEASKD
                     YIGPAVVNIYADDIILENLTVENTQPEIEPHAFAIYGKGTRTILRNCNLLGKGGDTVS
                     LWNYQQGLYYHSNCYFRGAVDMVCPRGWCFIRDSQFFEVKTSATIWHDGHYDPDQKFV
                     IVNSTFDGVPDFQLGRHHYDAQFFLINCNFSENMADQPIYLKTYDDVSRNNPYLWGER
                     KYFFNCRKAGKAFDWYRNNLATAPGKPQPEEITPAWTFADRWDPESSAPVTVTAYRID
                     GRTVELTFSESVTVGGNPIFMSQTGFKFSIMPQRFTDNCRLIFFAERPISQKDLTGEM
                     RLVEGKIIASQAYVTERGVARNFRIINN"
     gene            complement(4350..5759)
                     /locus_tag="PLI55_08260"
     CDS             complement(4350..5759)
                     /locus_tag="PLI55_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020528372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HPB00862.1"
                     /translation="MANCNEINRQSTDQSSLIKQIRLSILSLNHTDFYLILFLGLFIV
                     CQSTAFAQQLAFPGAEGFSRFTSGGRGGKVIAVTNLNDKGPGSLRSAIETNGARTIVF
                     RVSGTIALKSELTIQNGNLTIAGQTAPGDGICIRNYPLIIAADNVIIRYLRVRLGDEC
                     KVEGDAISAIFQKDIIIDHCSFSWGTDEVASIRDNVNSTVQWCLISESLTHSIHHKGD
                     HGYGGIWGGQGATFHHNLIAHNSSRNPRFNGSRYSHKPEAEYVDFRNNVIFNWGFNSA
                     YGGEGGHQNIIANYYKAGPASECPDRIVEPWDSTGQWYVAENFVFGFPAITADNWAGG
                     VQGKFRNFGRVDQPFAAAPVVTHSAEAAYELVLANCGAVLPKRDAVDERLVAEVRDGI
                     VHFGKNGIIDSQKEVGGWPTLNSTTPVKDADQDGMADDWELANGFNPNDPNDHNGDFD
                     GDGYTNLEEYLNSLDKLFY"
     gene            complement(5743..7152)
                     /locus_tag="PLI55_08265"
     CDS             complement(5743..7152)
                     /locus_tag="PLI55_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HPB00863.1"
                     /translation="MKKLSPVHRHLRLNLTIIILLGMICTWGIAQTPESGWNQVPAIL
                     EKIVPPEFNARDFNITDYGAIGDGQFDCTQAFVKAIDACHKAGGGRVVVPSGKFLTGA
                     IHLKSNVNLYITREGNILFSRDIHKYLPVVFTRFEGVECLNYSPFIYAYEQENLAVTG
                     SGTIDGQADNETWWNWVMNKKFGWKEGMPNQEKDRDLLMEMADKNVPVKERIFGEGHY
                     LRPNFIQFYKCKNILIEGVTLKQSPMWEIHPVLCENVTVKKVTVISHGPNNDGCDPES
                     CRNVLIKDCYFDTGDDCIAIKSGRNGDGRRLKAPSENIIIQDCRMKDGHGGVVIGSEM
                     SGNVRNVFAEDCIMDSPNLDRALRIKTNSLRGGIVENIYMRNVTVGVVSDAVIRIYFY
                     YAEGDVGQHTPIVRNIYVENVTSQASDYALFLQGYERSPITNVQLSNCRFNGVKKPNI
                     IKNVEGLKMKDVYINGELQ"
     gene            complement(7197..8420)
                     /locus_tag="PLI55_08270"
     CDS             complement(7197..8420)
                     /locus_tag="PLI55_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPB00864.1"
                     /translation="MKVKNNLKKKHFISGLILITLIPLIGRAALNQEIKPTSAWSVRM
                     AESVIQRHPQPWLLESPEKPKWEYTYGLVMKSILDVWEKTGEQKYFQYVESYYEYFID
                     DSGRILTYRPEEYNLDRINAGKPLFRLYAETGKVKYQKALMNLRQQMATHPRTKEGGF
                     WHKKIYPHQMWLDGLYMAGPFLAQYAVVFNEPALFDDVVKQFVLIEEKTRDPQTGLLY
                     HGWDESRQQAWANPETGRSPNFWGRAMGWYAMALVDVLDFLPADHPGRPEIVAILKRL
                     ADAVVKVQDPATGVWYQVLDKGGQPGNYLESSASCMFVYSLAKGVRLGYLDPVYLDAV
                     KKGYSGILNQFIEVDSAGLVNINQACAVAGLGGKPYRDGSYDYYVSAKTARNDPKAVG
                     PFILASLEMEMLNEK"
     gene            complement(8508..>8726)
                     /locus_tag="PLI55_08275"
     CDS             complement(8508..>8726)
                     /locus_tag="PLI55_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB00865.1"
                     /translation="TTQIAFDLDKSGYTTLTIYNLLGQMVDKPVAKHLPAGSYRITFD
                     ASHLAGGMYFYKLECAGQVNVRKMMLLK"
BASE COUNT         2434 a         2100 c         1770 g         2422 t
ORIGIN      
        1 gacgattctg accgataata gctaggactt ccaaccatcc ctatgaacta tgtctggaat
       61 tgtcggatat tgaacatcgt aatcccgcct gggcgggacg ccatccgcaa accaatggtt
      121 ttgtagaacg attcaatcgt accgtcctaa atgaattttt ccgtccggct ttccgaacta
      181 aaatgtacga atcagtcgaa gctttacaaa aagatctgga tatatggctg agagaataca
      241 atgaacaaag accccaacgg ggttaccgca acatgggcaa acgcccaatt gatcgcatca
      301 atgaatattt gaaaattgtt tcgccagaag gttaatcata cccatttttt gccctcacaa
      361 tttaatccac aggcacccca aacaacggct gaatatgtct ttggtagaga ttttcggtca
      421 cgttcggggc aataaattca gcgtattttt tgagagccga aaggtagcgc tcgcccatct
      481 cagccgcaat cttatagccg acgtgtaata gctggcgcag atgcgggtta taggcttttt
      541 cactctgatt atgacgtaaa gctgatacat actcgcgaga agtccaaccg ttgacgaccg
      601 aaggcgccgg caatttagtc cgatcaatgt ccacaacagt tgcatagggc ttgactaatt
      661 cctcaaaacg ttcataagca cggcaataga tttcacgcgc tatttccaag ccttcgccgc
      721 cagccgcggc taaaccaatc agttcctcca gccaggttgt cccggcagtc ttgagatgca
      781 gtccggcatt agtttttgta ataatgctat taatatgtgg atagagtgag aacttatcac
      841 tgccggagtg aacgctaagt ttcagcccat ccggcaaaga aaattccttg atcgcaaatt
      901 ttaccacggc gacatcctgc tcgaattcaa ccgcaaattt tgccgggtcg ccgacgtagt
      961 caacgccctt attgaaacgt cccgaaaatt tcggcgcaat tgtagccagc ggcagttcct
     1021 gccaagccgc catactcaga atgaaaaata attccaacgg gctttgtggc ttgtcagttt
     1081 catcaacaga aagttcaatc acataatcgt gttgacaatt atcctgaatg tagtgataag
     1141 tatcagccgc ggctaatact gccgctaagt atttgcgggc gatcttttcg atagatttat
     1201 gatcaactgc gattttctgc agaatgcccg gaataccaaa aggttgcgta tatttttcag
     1261 caaattttaa aaattcccga atatcgtttt cattgggcgg aacaccgatg gcatcagcca
     1321 catccagcgt aaaaaaatca gaggattcga taaaatgggg cacggttttg agattgatat
     1381 gatcggcatc aacaaaatac cccttcgacc agttcaaagc ctggaccgct ttctcggccg
     1441 aatgacgcac atcggccggt tgactgccga ttatattatg ttcgcgaaaa gacttattcc
     1501 agactggcgt gatattgacg cccttttcgg cggctttgac tagcgcggtt aattgggctt
     1561 cagcttgatg cccgaaacga tcccctatgc ccagtgaata ttttttcaat ttcattttga
     1621 ctatttcctt ttagcaatta aaaaaaccgg tctgtattta cgaattctta cgaatttaaa
     1681 aaatgaaaat tccagaaatt aatgttttcc gatgttctat tttatcgtaa taattaaatc
     1741 attatccagc attgccaccg tttcattcag ataatctgcg aactggcttt cggttttaat
     1801 gccgtccggc tcctgttccc aggcggctaa gaaataatac gtcaattttc ccgccgtcgg
     1861 tttcaaaatc acaacatagc tattttcatc atcggttatc tggatcaaat cttggcgacg
     1921 gaaaagcacc gccagtccta atttatcatt cgccagactc tgtttaccat aggtggcaaa
     1981 gtacatccag tcacgatttt gatcagtcga actaagtagc gacacgtttt ccattttcac
     2041 aatgccggta cacagattgg gcggctcgtc gttaatttgg atatcgtgac gggtcaagcg
     2101 actaccggcg gcaatcgaga gttcggacac caaatcatat ttttgacctc cgatttgcca
     2161 gccataataa cgggtacgga tgcgcgattc aagtatgcca tcctgtaaaa tttcgcaaat
     2221 tatactgtca gtaacggcaa tccgctcggc tttcccttca taccacatac ccaacgaacc
     2281 aagaccgagc gattcgccga ctttcagcac atccattccc cagggacaca tactatggta
     2341 cgattcaaag ccatcctggc cgatttcttg taaaaccata tcgggtactt tcttgccaaa
     2401 gacatctatg gcattgcgcc agtcgagata aaaacgatag ccgactttat cggactccca
     2461 acccggtcct tcataacgaa tgaacagcga atggtcggta tgttcgggtg gtacccgcaa
     2521 atattttaca ttctgaaatt gtccaccttc ataaatccga ttgacaaaac gtccgccgac
     2581 tttatgagat aattccgcct gcgttcgctt gggataattg tgagtaatcg ttccttcctt
     2641 agcataatga attgtgaagg ttttcttctc ggaaggagca aaatcggtaa cgaataaaat
     2701 ttcggttttc tccgcattta gctgagaggg gatttccgtc tcaccggcga aaattaccca
     2761 agcgtccgga ttaaaatccg ggtatttggc ttttatgtca gccagattta ttctaaccat
     2821 cgcgtcttta cgaacctgtt tggtaggatt ttttacaatt attttaaagg aaagcggcag
     2881 gtctggacgc tctttagcag aacggttatt acaagaaaaa acacttgtta aaaacagcat
     2941 tgctacgatt attaatcttg gtttagacat tggtgactcc ctgatattat ttttattttt
     3001 taacctaaaa ttcatatcta attgttaata attctaaaat tacgagctac gcctcgctcg
     3061 gtaacgtaag cttgcgaggc aattattttc ccttctacaa gcctcatttc acccgtcagg
     3121 tctttttggg atattggccg ctctgcaaaa aatatcaagc ggcaattgtc ggtaaaacgc
     3181 tgaggcataa tgctaaattt aaagccggtc tgactcataa aaatcggatt gccacctacc
     3241 gtcacacttt cactaaaagt taattcaact gttcgaccat caatgcgata agctgtaacc
     3301 gtaaccggcg cgcttgattc cggatcccag cggtcagcaa aagtccaggc tggtgttatt
     3361 tcttccggtt gcggttttcc aggagctgtc gcgagattat tacgatacca atcgaaagct
     3421 ttgcccgctt tccggcaatt aaagaaatat ttacgttcgc cccacaaata aggattattg
     3481 cgactgacat cgtcataagt tttcagatag atcggctgat ccgccatatt ttccgaaaaa
     3541 ttacaattga taaggaaaaa ttgagcgtcg taatgatggc gtcccagctg aaaatccggc
     3601 acgccgtcaa aagtggaatt tactataacg aatttctgat ccggatcgta atgaccatca
     3661 tgccagatcg tcgccgaggt ctttacctcg aaaaattgcg aatcgcggat aaaacaccag
     3721 ccgcgcgggc aaaccatatc aaccgccccg cgaaaataac aattagaatg gtaatacaaa
     3781 ccctgctgat aattccagag cgaaacggta tcgccgcctt ttcccagcag gttgcagttc
     3841 cgtaaaattg tgcgagttcc ttttccataa atagcgaagg catgcggttc aatctccggc
     3901 tgagtatttt ccaccgtcaa attttccaaa ataatgtcgt cagcatagat attgacgact
     3961 gccggtccga tataatcctt gctggcttcc caatcagtgc gtttctgaca gaaacggata
     4021 atggtgctgt cgcgattttc accgcgaaga gtgacataat tctggtctaa tcgaattttt
     4081 tcctcgtaaa taccatttcg aataaaaata accacccgtt gataagccat atccggtaaa
     4141 gccgccagcg ccgccgtgat cgaggtaaat tgtcccgcgc cactttgatc aacaatcatc
     4201 aggttatcga attcttcgga ggcagtcccg atttgagcca gacaaatcag tagcgatgtc
     4261 aaaagggcaa tttttcgcat aaaagacctt cagttataac atcgctacca atttcctcaa
     4321 tcactacatt tatctttttc agaaacattt taataaaata atttatccag actatttaaa
     4381 tattcttcaa gattagtgta accgtcaccg tcaaaatcgc cgttatggtc gttcgggtcg
     4441 ttgggattaa agccatttgc cagttcccag tcgtctgcca taccatcctg atctgcgtct
     4501 ttgaccggtg tagtagaatt caaggtcggc cagccgccaa cttccttctg agaatcgata
     4561 atgccatttt taccaaagtg aacaatccca tcccgaactt ccgccaccag acgttcatcc
     4621 accgcatcgc gtttgggcaa aacagcaccg cagtttgcca atactaattc gtatgccgct
     4681 tccgccgaat gagtaacgac cggcgccgcg gcaaatggct gatcaacgcg cccaaaattg
     4741 cgaaacttac cttgtacacc gcccgcccaa ttatccgccg taatggctgg aaatccaaac
     4801 acaaaatttt cggcaacgta ccactgaccg gtgctgtccc atggctcgac aattcggtcg
     4861 ggacactccg aagcgggacc ggctttataa taattggcga taatattctg atggccacct
     4921 tcgccgccgt aagcactatt aaaaccccaa ttgaaaatca cattattacg gaaatcaacg
     4981 tactcggctt caggtttgtg actatagcga ctgccattga agcgcggatt gcgactggaa
     5041 ttatgcgcaa ttagattatg gtgaaaagtc gcgccttgac cgccccagat gccgccataa
     5101 ccgtggtcgc ctttatgatg aatcgaatga gtaaggctct cgctgattag gcaccattga
     5161 acggtggagt tgacattatc gcggatcgaa gcgacttcat ccgtgcccca gctgaacgaa
     5221 caatgatcta taataatatc tttctggaaa atggcagaaa tggcatcgcc ttcaacttta
     5281 cattcgtcac ccaacctgac acgaagatag cggattatga cattatcagc cgcaattatc
     5341 agagggtaat tacgaataca aataccgtcg cccggcgccg tttgaccagc gatcgttaga
     5401 ttgccattct gaatagtcag ttcggatttt aaagcaatcg tgcctgaaac cctgaaaaca
     5461 attgttcggg cgccattggt ttctattgcg gatcgcaaac tgcccggacc tttatcattt
     5521 aagttcgtta cggcaatgac tttaccaccg cggccaccgg aggtgaaccg gctaaatcct
     5581 tccgcgccag gaaatgccaa ttgctgagca aatgccgttg attggcagac gataaacaaa
     5641 ccgagaaaca agatcaggta aaaatcggta tgattcaaag atagtattga taaacgaatt
     5701 tgttttatta aggacgattg atcagttgac tggcgattga tttcattgca gttcgccatt
     5761 gatatagacg tccttcatct ttaagccttc cacattttta ataatgttgg gcttttttac
     5821 tccgttaaaa cgacaattac taagttgcac gttagttata ggcgaccttt cataaccttg
     5881 taaaaataaa gcataatcgc ttgcctggct ggttacattt tcgacgtaaa tgttacggac
     5941 aatcggcgta tgctgaccaa cgtctccttc ggcatagtag aagtaaattc gaataaccgc
     6001 gtcactgaca accccgacag tcacgttgcg catgtagata ttctcgacga ttccaccccg
     6061 cagggaattg gttttaatcc gcaaagctcg atccaggtta ggactatcca taatacaatc
     6121 ttccgcaaag acattccgga cgttgcccga catttcactg ccgattacta caccaccatg
     6181 cccgtctttc attcgacagt cttgaataat gatattttcg ctcggggctt tgagacgtcg
     6241 tccgtcgcca ttgcgtccgg attttatggc gatacaatca tcgccagtat cgaaataaca
     6301 gtctttgatc aagacattgc ggcaactttc ggggtcacag ccatcgttat taggaccgtg
     6361 gctgatgacg gtaacttttt tgacggttac gttttcacac agcaccggat gaatttccca
     6421 cattggtgat tgcttaagcg taacgccttc aatcagaata tttttacact tataaaactg
     6481 gataaaattc ggccgtaaat aatggccttc accgaagata cgttctttta ccggtacatt
     6541 tttatctgcc atttccatca ataaatcacg gtctttttcc tgattcggca ttccttcttt
     6601 ccagccgaat ttcttgttca tcacccaatt ccaccaggtt tcattatcag cctgaccatc
     6661 aatggtaccc gatccggtga cagccagatt ctcttgttca taggcataga taaaaggcga
     6721 ataattcaga cattccaccc cttcaaatcg ggtaaatacg accggcagat atttatgtat
     6781 gtctcggctg aatagaatat ttccttctct ggtaatatac agattgacat tacttttcag
     6841 atgaatagca cccgtcagaa atttaccgga cggtacgacg acgcgtccgc ctccggcttt
     6901 atgacaggca tcaatggctt taacgaaagc ctgagtgcaa tcgaattgac catcaccgat
     6961 agcgccgtaa tcagtgatgt tgaaatctct ggcattgaat tcggggggaa cgattttttc
     7021 cagaattgct ggtacttgat tccaacccga ttcgggagtt tgagcaattc cccaggtaca
     7081 aatcattccc agcagaatga taattgtcag attcagacgt agatggcgat gaacaggcga
     7141 taattttttc attttgtact agttacactt tcatttattt attcaaataa ttattttcac
     7201 ttttcattca gcatttccat ttccagactt gccagaataa atggcccgac ggctttggga
     7261 tcgttgcgcg cagttttggc gctcacataa taatcgtaag aaccgtcacg gtaaggttta
     7321 ccgcccagcc cagctactgc gcaagcttgg ttgatattga ccagtccggc ggaatccact
     7381 tcgataaact gattcaaaat gccggaatat cctttcttaa ctgcatcaag gtaaaccgga
     7441 tcaagatagc cgagccgaac gcctttggct aacgaataga caaacataca ggacgccgag
     7501 gattccaaat aattacctgg ctggccacct ttatccagaa cttgatacca aacgccggtt
     7561 gccggatctt gaactttaac gacagcatcc gctaatcgtt ttaaaatagc aacgatttct
     7621 ggtcgccccg gatgatcggc gggtaaaaaa tcgagcacat caaccagcgc catcgcgtac
     7681 catcccatcg cgcgccccca aaaattggga gaacgaccgg tttctggatt agcccaggct
     7741 tgctgacgac tctcgtccca gccatggtag agcaaaccgg tttgtggatc gcgcgttttt
     7801 tcttcgatta aaacgaattg cttgacgaca tcatcaaata acgccggctc gttaaagacc
     7861 accgcatatt gagctaaaaa aggtcctgcc atatataaac catccagcca catttgatgc
     7921 ggatagattt tcttatgcca gaaaccacct tctttagtgc gcggatgagt cgccatttgt
     7981 tgccgcaaat tcatcagcgc cttttggtat ttgaccttgc cggtttcggc atagagtcga
     8041 aaaaggggtt taccagcatt gatccggtca aggttatact cttctggacg gtaagttaaa
     8101 atgcggccgc tatcgtcaat aaaatattca taataggact cgacatactg gaaatatttt
     8161 tgttcaccgg ttttttccca aacatccaga atggatttca taaccagtcc gtaagtatac
     8221 tcccatttag gtttctccgg actttcgagt agccagggtt gcggatgccg ctggataacc
     8281 gattccgcca tccgcaccga ccaggctgag gttggtttaa tttcctgatt caatgccgct
     8341 cttcctatta gaggaataag ggtaatcaga atcaatcctg atataaaatg tttcttctta
     8401 agattatttt ttactttcat tttaactcaa ttggtaaaaa tatgggttgg gaggacatta
     8461 catcctccca acccattacc catttcagat taatattcta ttagaaatca cttcaacaac
     8521 atcattttac gaacattaac ctgaccagca cattccagtt tataaaaata cataccacca
     8581 gctaaatgac tggcatcgaa agtaatccgg tacgaaccag ccggtaagtg tttagcaacc
     8641 ggtttgtcaa ccatttgccc caacaagtta tagatcgtta gagtcgtata accggattta
     8701 tccagatcaa atgcaatctg agtagt
//
LOCUS       DAOOMU010000088         8712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_9458_length_8712_cov_21.090563, whole genome shotgun sequence.
ACCESSION   DAOOMU010000088 DAOOMU010000000
VERSION     DAOOMU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9458_length_8712_cov_21.090563"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..447
                     /locus_tag="PLI55_08280"
     CDS             73..447
                     /locus_tag="PLI55_08280"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPB00866.1"
                     /translation="MKRSTLVRFAIFFSLTLVLIFFSGCATEAQRHYDLGKWYYEKGL
                     INEAILEFKAATQKDPDFYQAHQSLAIAYAKKGWYDYALKEAEISFDLHPSDESYNLI
                     QIIRHKKILEPAFESALTDTLQ"
     gene            583..>1105
                     /locus_tag="PLI55_08285"
     CDS             583..>1105
                     /locus_tag="PLI55_08285"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HPB00867.1"
                     /translation="MKHILSVSRRSDIPAFYGEWFLQKVREGSVTLSSPFSNRIQSVS
                     LCPEDVSAIVFWSKNYQPFLPILTQIKSLYHGRFLFHLTMNGFSGPAKILLEPGAPDT
                     EKALKTAHLLCDEYSPEKILWRFDPIIFSNLTPPEERLEAFSKLAEQLRGLTQRCYVS
                     FVDLYGKVKRRFNA"
     assembly_gap    1106..1205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1283..2140
                     /locus_tag="PLI55_08290"
     CDS             1283..2140
                     /locus_tag="PLI55_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPB00868.1"
                     /translation="MSKTTKTSSRDSSRTLSRYLEEISHFSPLSPEKEIELARRIKNG
                     DRQAAKELTEANLRFVVSVAKDYQGQGLPLMDLINEGNLGLLKAVERFDETRGFKFIS
                     YAVWWIRQSILQALSEQSRLVRLPMNRVGTLTKIAREAEVLEKQNERSPRYDEIAAGL
                     DVDREVVNDTLHIARRHRSLDEPMGDSDSNSLIDVLTDENVTDPDWQLMVESLSHDIN
                     TSLAALNKREREVLKMYFGIGSEYALTLNEIGEHFNLTRERVRQIKERAIRRLRHKSH
                     SSLLKTHLG"
     gene            2339..3694
                     /gene="glmM"
                     /locus_tag="PLI55_08295"
     CDS             2339..3694
                     /gene="glmM"
                     /locus_tag="PLI55_08295"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPB00869.1"
                     /translation="MLIESLSGVRGYDTDLTKELISSYAAALKELLACRRVVLGRDTR
                     ASGVRIREQLTEALRRYGVAVIDLGICPTPTVEMAVVQHRADAGVVITASHNPLPWNG
                     LKFLGADGLFLDPEQMLWIKQNRQSLLAKYSDAHFSPGDLIFYEWANRDHINAILKLP
                     YVEIESIRRQHFKVAVDAVNGAGSKIIPELLKEFDCEVIAIHCDPHQPFPHPPEPLPE
                     NLTDLCQAVKDNSADLGLAIDPDGDRLAIVSDKGVPISEEYTLVLATKLVLSKTGKKN
                     QKVVANLSTTRALEDLAARYNAKVIRTPVGEINVARRMQMEKALIGGEGNGGVILPEA
                     HLGRDSLVGTALVLQLLADEKRSLSAVMPTLPQYFMVKRKIPRGKADFNEIMNHLIQS
                     VDNAEINTEDGLKFSWSDRWVHIRTSNTEPIMRIYAEAPTSQAADQLADRFVTLLQKF
                     I"
     gene            3728..4429
                     /gene="deoC"
                     /locus_tag="PLI55_08300"
     CDS             3728..4429
                     /gene="deoC"
                     /locus_tag="PLI55_08300"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPB00870.1"
                     /translation="MTYASIINPEEARLAIAHLIDHTLLKPEATPNDIVRLCEEALKY
                     QFATVCVNPSFVELCANCLYNSKVAVATVIGFPLGANTMEIKAAEVEQALNHGATEFD
                     MVINIGRLKAGDLEYVEDDIHAVVDAADGNLVKVIIETALLTDEEKKLACHLALKAGA
                     HFVKTSTGFSKYGATVEDVRLIRSIVGNQMGVKAAGGIRTYSDAILMIEAGANRIGTS
                     AGVQIVQESKQFKNH"
     gene            4642..5514
                     /locus_tag="PLI55_08305"
     CDS             4642..5514
                     /locus_tag="PLI55_08305"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPB00871.1"
                     /translation="MGIPGLTIIGESINDSVPSTHDLFQANDLDGIVGLARFQAEKGA
                     AYIDVNVGRRSPAFMAEVVQKIQQHVSVPLSIDTPDPEIALAGLEAYNPKLAGNRLPV
                     LNSISEARLEIFDLYSKQPFIPILLITEGKNESGEIVMNKTPEQNYSTAKSLVEIARE
                     RIQGVANNRLILDPGIAPIGSDMEGNFKRLMKVMEMIHQDPDLAGINMSVGLSNFTVM
                     LPSKKSDGSPVKSPLESAFLTLAMPLGLNFIVGSVKRKYEILPDDHPAIQCLKNILAS
                     DGLNAVLQIMNFIA"
     gene            5556..5999
                     /locus_tag="PLI55_08310"
     CDS             5556..5999
                     /locus_tag="PLI55_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPB00872.1"
                     /translation="MDLVTFDKPPISARERKLSRFMLFFEDLIKVPVFHCQHCGECLL
                     SSTGFVCSQRCPKRLRNGPCGGTRDDGHCEVFPERKCIWYRIYRRSKLLGRIKLLYKI
                     NRIHNWNLEHTSAWLNVFRKRIEGLVLFVPKNLDIENINDTQRKN"
     gene            5977..6867
                     /locus_tag="PLI55_08315"
     CDS             5977..6867
                     /locus_tag="PLI55_08315"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPB00873.1"
                     /translation="MTLREKINSGKFIVCGEIGPPQSCDGDVIRRKAQYFKGIADAVN
                     ITDNQTAIPRLSSIASAKILLDEGVEPIIQMTCRDRNRLAIQSDLLGAAALGIRNVLC
                     LTGDYQKLGDNPEAKGVFDLDSIQLIATVAQMNSGYLLSGREMKKAPEFLIGGAANPF
                     AVPFEMRLIRLYKKVKAGVNFIQTQPVFDPELFIRWMEKVVEMGLHEKVAILAGVMPV
                     KSVNTLLHMREDVPGVKINDVYIRRMEKAADPQAEGIKIAIEIIQTVKNIKGVRGVHI
                     MPFMWESIMPTVVKEAGLCN"
     gene            6885..7550
                     /locus_tag="PLI55_08320"
     CDS             6885..7550
                     /locus_tag="PLI55_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HPB00874.1"
                     /translation="MQDIIEKIALCVEKGKISRNHPFPPDMKDQDGADEIAAAALKEG
                     VPPDVLLQASMLGMDRIGKKFSENLVFVPHLLVAAQAMNAVLKHLQPYLEAGSVKRKG
                     KFVIGTVAGDLHDIGKNLVAMIVRGGGFEVIDLGTDVSTQKFLSAVEQHPGCFVGLSA
                     LLTTTMLNMGKIVSAIRETFPTTKVLIGGAPVTEEFRAQIGADFYSPNPQQAVEYLNQ
                     FVS"
     gene            complement(7638..8537)
                     /locus_tag="PLI55_08325"
     CDS             complement(7638..8537)
                     /locus_tag="PLI55_08325"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HPB00875.1"
                     /translation="MKRAIYITISLALVISVFISELLGEGKPFYQMSNPEIDSLLTIV
                     STQNLTITDRINLFSDKFLGMPYNQNCAGDGPYALYEPWPLVNFKETNCMVFCEHVLA
                     LSISDSWDNFFNNLQQIRYQDGIIGMRTRNHYTMADWLPENSWLLEDVSHQVGGQYIQ
                     QTTRTISHRKFFEDKGIADLRYVKPDRTITLEYVPKEYLFKVENNICNGDILVLIFAN
                     KSDIFSAHMLISVVKNGKLFIRESSSRKMTTCETPFTEWGKSIQSQDQYAGLCFIRIR
                     AELNTPGQIILPWEIDELKKNHE"
BASE COUNT         2422 a         1773 c         2023 g         2394 t
ORIGIN      
        1 tttttacctt gtttttatgc ctttataatt gaaaattaag cgtaaatgac gagtaattcc
       61 aggagaaata atatgaagcg ttccaccctt gtccggtttg ctattttctt ttctttgacc
      121 ttagtgttaa tatttttctc cgggtgcgcg acggaagcgc agagacatta cgatctcggc
      181 aaatggtatt acgaaaaagg cttgatcaat gaagccattc tcgaattcaa agccgctacc
      241 cagaaggatc ccgattttta ccaagcccat cagagcctgg cgatcgccta cgccaaaaag
      301 ggctggtacg attacgctct gaaagaagcc gaaatatcct tcgatttaca tccatcggat
      361 gaaagctaca atcttatcca gattatccga cataagaaaa ttctcgaacc ggctttcgag
      421 tcagccttaa ctgatactct tcaatagtag attaaaacca cgactattta ctaccgggcg
      481 aggagcaatt gcttccggtc gttaaatttg cgtcattttg aaagggagga tttttaaaat
      541 tttatttcgt attctttgcg atctttgcgg taatttatac cgatgaagca tattctatcc
      601 gtcagtcggc gtagtgatat tccagctttt tatggggagt ggtttctaca aaaagtccgc
      661 gaaggcagtg ttaccttgtc cagcccattt tccaaccgaa tccagtcggt ttctctatgc
      721 cccgaagatg tcagtgcgat tgtattctgg tcgaaaaatt accagccatt tttgccgatc
      781 cttacgcaga taaagtcgct ttatcacggt cggtttctgt ttcacttgac gatgaatggt
      841 ttcagcggtc cagcaaaaat cctgctggaa cccggtgcgc ctgatactga aaaggctttg
      901 aaaacagctc atttattgtg tgatgaatat agcccggaaa aaattttatg gcgcttcgat
      961 cccatcattt tttccaatct aacaccgcca gaagaacgtc ttgaggcttt cagtaaatta
     1021 gccgagcagt tacgggggct gactcagcgt tgttacgtga gtttcgttga tctctatggc
     1081 aaagtcaaac gccgttttaa cgccgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnattaa atgcgaatgt aattgacaaa atagaaaaat aaccttattt ttaatcttga
     1261 atgaaaaatt aaaaagtaga aaatgagtaa gacaaccaaa acctccagcc gcgattccag
     1321 ccgaaccttg agtcggtatc tggaagaaat cagccatttt tcaccgttgt cgcctgagaa
     1381 ggaaatcgag ttggcgcggc ggatcaagaa cggtgatcgt caagcggcta aggaattgac
     1441 tgaagccaat ttaagatttg ttgtcagcgt agctaaggac taccaggggc aaggattacc
     1501 tctgatggat ttaattaacg aaggcaatct tggcttgtta aaagccgttg agcgtttcga
     1561 tgagacccgc ggtttcaaat ttatttccta tgccgtttgg tggatcaggc aatcaattct
     1621 gcaagcgctt tctgaacaat cgcgtttagt gcggctaccg atgaatcgag tcggcactct
     1681 caccaaaatt gcccgggaag ccgaagtgct cgagaaacaa aatgaacgct caccgcgcta
     1741 tgatgaaatt gccgccggtc tggacgttga tcgcgaagtt gtgaacgaca ccctccatat
     1801 cgcgcgccgt caccgctccc tggatgaacc tatgggcgat tctgatagca atagtctgat
     1861 tgatgtactg acggatgaga atgttaccga tccggattgg cagttgatgg tagaatcgct
     1921 aagccacgat atcaatacct cgttagccgc cttgaataaa cgtgagcgag aggtgttgaa
     1981 aatgtatttc ggaattggca gtgaatatgc gctgactttg aacgaaattg gtgaacattt
     2041 taatctgacg cgtgaaagag tacgccagat taaggagcga gctattcgtc ggcttagaca
     2101 taaatcgcat agttcgttat tgaaaacaca tctgggttaa ttctgttttc aatataagat
     2161 tagaaaaata gtttctgtcg gtgagtagag gggaaattat ttatccagaa agaagtatac
     2221 tagatctaag actggcaact tgtgaacttg ccggattgat actatattat taaaagtatg
     2281 cttgattatt acttagttaa aaatataaat atatctgaca ggagggaagg aatcgaaaat
     2341 gttgattgaa agtctatcgg gggtgcgcgg ttatgatacg gacttgacca aggaactaat
     2401 atcgtcgtat gccgccgccc taaaggaatt gctcgcctgc cgaagagtcg tccttggacg
     2461 tgatacgcgc gccagcggcg ttcgcatccg ggaacaatta actgaggctc tgcggcgcta
     2521 tggggttgcg gtaattgacc tgggaatttg tcctacaccg acggtcgaaa tggctgtagt
     2581 tcaacatcga gccgatgccg gcgtcgtcat aactgccagt cacaatcctt tgccctggaa
     2641 tggattgaaa tttctgggag ccgatgggct ttttcttgat cccgagcaga tgctctggat
     2701 taaacaaaac cgccaaagtc ttttagcgaa atacagtgat gcccatttta gtccgggcga
     2761 tttgatattt tatgagtggg caaatcgtga ccatattaat gctatcttga aattaccgta
     2821 tgtcgagata gaatctattc gtcgtcaaca ttttaaagtt gccgtggatg ctgtcaacgg
     2881 tgcgggttcc aaaataattc cagaattact taaagaattt gattgtgaag taattgcgat
     2941 tcactgcgat ccccatcagc cttttccgca tccgccggaa cccttgccgg agaatctgac
     3001 cgatttgtgc caagcggtaa aggacaactc ggctgatcta ggcttagcaa ttgatccaga
     3061 cggtgaccgc ttagccattg tctcggacaa aggtgtgccg atttccgaag aatatacctt
     3121 ggtgctcgcg acaaaactgg tattgtcaaa gaccggcaag aagaaccaaa aggttgtcgc
     3181 taacctttcg actactcgag cgttggagga tcttgccgct cgatataatg ctaaggtaat
     3241 tcgtactccg gtcggcgaaa tcaacgttgc ccggcgtatg cagatggaaa aggctttaat
     3301 cggcggtgaa ggcaacggcg gggtaatttt accggaagct catctgggac gtgattcgct
     3361 ggtaggaaca gctttagttt tgcaattgct ggcggatgaa aagcgctcac tttcagcggt
     3421 gatgccgact ctgccgcaat attttatggt aaagcgtaaa attcctcgcg gaaaagccga
     3481 ttttaacgaa attatgaatc atctgattca gtctgttgac aatgccgaaa ttaacacaga
     3541 agacggtttg aaatttagct ggtcggatcg ctgggtgcat atccgcacgt ccaataccga
     3601 accgattatg cggatttacg ccgaagcgcc aaccagtcag gcggctgatc aattggctga
     3661 tcgtttcgtc acattattgc agaaatttat ttaaagctaa tatatttaaa ttaataagga
     3721 aattatcatg acttatgcct cgataattaa tcccgaagaa gcgcgcctcg ctatcgcgca
     3781 tttaatagac catactttgt tgaaaccgga agcgacgccg aacgatatcg ttcgcctttg
     3841 cgaagaagct ctgaaatatc agtttgcaac cgtatgcgtc aacccatcct ttgtggaatt
     3901 gtgcgccaat tgcctctata attctaaagt cgccgtagcg accgtaatcg gctttccgct
     3961 gggtgctaat acgatggaaa tcaaagccgc tgaagtggag caagcgctaa atcatggcgc
     4021 caccgagttc gatatggtca tcaatatcgg tcgcctgaaa gccggtgatt tggaatacgt
     4081 cgaagatgac attcatgccg ttgtggacgc ggctgatggt aatttggtga aagtgattat
     4141 cgaaaccgca cttctaactg acgaagaaaa aaaactggct tgtcatctgg cgctcaaagc
     4201 cggtgctcac tttgtaaaaa cttcgaccgg tttcagtaaa tacggggcaa cagtcgaaga
     4261 tgtgcgcctg atacgaagca ttgtcggtaa ccagatggga gtcaaagcgg ctggcggcat
     4321 tcgcacttac tccgatgcta tattgatgat cgaagcgggc gccaaccgca tcggaaccag
     4381 cgccggggta cagatagttc aggaaagtaa acagtttaag aatcattgaa tcttcgacga
     4441 tgagcatcag attatttgag tcggtgcggt aagattggaa tgagagcagg gagggcttta
     4501 taatgttcct aaatacaacg aagctcccat tctttgaata aaattctctg taacgaacac
     4561 agtaattaat ccttggaaaa aacagttgaa atgttgaaaa tagcggctat gaatttacta
     4621 taaaatgaaa ggtctttata aatgggtatc ccaggactga ctattatcgg tgaatcaatt
     4681 aacgattcgg tgccttcgac tcacgatctt tttcaggcga acgacctgga tggtatcgtt
     4741 ggactggctc gtttccaggc tgaaaaggga gcggcttata ttgacgtgaa tgtcggtcgg
     4801 cgttcacctg cttttatggc ggaagtcgtc caaaaaatcc aacagcacgt ttccgttcct
     4861 ttatcaattg atacgccaga tccggaaatc gctctcgcag gtctggaagc ctacaatcct
     4921 aaactggctg gtaatcgttt gccagtcctg aattcaattt cagaagctcg tctggaaatt
     4981 tttgatttat actccaaaca gcccttcatt cccattttac tcattactga aggaaaaaat
     5041 gagtcgggcg aaatcgttat gaataaaaca cccgaacaga attattccac ggcaaagtct
     5101 ttagtagaaa ttgcccggga aaggattcag ggagtagcca ataatcgcct tatcctcgat
     5161 ccgggcattg ccccgatcgg cagtgatatg gagggcaatt tcaagagact gatgaaagta
     5221 atggaaatga ttcatcagga tcctgacctg gctggtatta atatgtcggt tggtttgagc
     5281 aactttaccg ttatgttgcc ctcaaaaaag tccgacggct cgccggtgaa aagtccgctg
     5341 gaaagcgctt ttttgacact ggcgatgccg ctgggtttga attttatcgt cggttcggta
     5401 aaacgtaaat acgagattct tccggatgac catccggcta tacaatgttt aaaaaatatt
     5461 ctggcatccg atggattaaa tgccgtcttg caaattatga actttatagc ctgattctta
     5521 ataaccgtta agtcgaattt tcaaaaaggg aaaatatgga tttagtaact ttcgataaac
     5581 cgccaatatc tgctcgcgag cgcaagttat cacgcttcat gctctttttt gaagacctta
     5641 taaaagtccc cgtatttcat tgtcagcatt gcggcgaatg tctgctttcg tcaactgggt
     5701 ttgtctgttc tcaacgctgt cccaagcgac tgcgcaatgg tccgtgcggc ggcacgcgag
     5761 atgacggcca ctgtgaggtt tttcccgaac gcaaatgtat ctggtatcgg atataccgac
     5821 gatcaaagtt attagggcgc atcaaacttt tgtataaaat taatcgaatt cacaactgga
     5881 atctggaaca tacttccgcc tggctgaatg tatttagaaa acgcatcgaa ggcttggttc
     5941 tatttgttcc taagaattta gatatagaga atattaatga cactcagaga aaaaattaat
     6001 tccggaaaat ttatcgtgtg tggcgagatt ggtccgccgc agagctgtga cggcgatgtg
     6061 attcggagaa aagctcagta tttcaaggga atcgccgatg ctgtcaatat taccgataac
     6121 caaactgcca tcccgcgtct gtcgagcatt gcttcggcta aaatattgct ggacgaggga
     6181 gtagaaccga ttattcaaat gacctgccgt gaccgcaacc gattggcgat tcaaagcgac
     6241 ctgctggggg cggcggctct cggtattcga aatgtgctgt gcctgaccgg cgattaccaa
     6301 aaattaggcg ataacccaga agccaagggc gtattcgatc tagattcgat tcaactgata
     6361 gccactgtag cccaaatgaa ttcaggttat ttactctccg gtcgtgaaat gaaaaaagcg
     6421 ccggaatttt tgattggcgg ggcggcaaat ccttttgccg tgccttttga aatgcgtttg
     6481 attcgtcttt ataaaaaagt caaagccggt gtaaacttta ttcaaaccca gccggttttc
     6541 gatccggagc tcttcattcg ctggatggaa aaagtagtgg aaatgggatt gcatgaaaaa
     6601 gtcgctatcc tagctggtgt tatgccagta aaatctgtca atactttact gcatatgcga
     6661 gaagacgttc ccggcgttaa aattaatgat gtatatattc gacggatgga aaaagccgcc
     6721 gatccgcagg ccgaaggcat taaaattgcc atagaaatta tccagacggt taaaaatatc
     6781 aagggagttc gcggagttca tattatgcct tttatgtggg aaagtattat gccaactgta
     6841 gtgaaagagg ctggtctatg caattaaaac taaggagtta gataatgcag gatattattg
     6901 agaaaatcgc gctctgtgtc gaaaagggga aaatttcacg caatcacccc tttccgccag
     6961 atatgaaaga tcaagacggc gctgatgaaa ttgccgccgc ggcgttgaaa gaaggcgttc
     7021 cgccggatgt tttactgcag gcttctatgc tgggaatgga tcggattggg aaaaaattca
     7081 gcgaaaatct ggtttttgtg ccgcatttgc tggttgccgc tcaggcaatg aatgccgtct
     7141 tgaaacattt gcaaccttat ctggaagccg gttcggttaa gcgcaaaggt aaatttgtaa
     7201 tcggcaccgt tgctggcgat ttgcacgaca tcggtaaaaa tctggtagct atgatcgtaa
     7261 gaggcggcgg ttttgaggtt attgatctgg gcaccgatgt ttccactcaa aagtttttaa
     7321 gtgctgttga acaacacccc ggttgttttg ttggacttag cgccttacta accactacca
     7381 tgttaaatat gggaaaaatt gtgtcggcaa tcagagaaac ttttccgact actaaggtat
     7441 taattggagg cgcaccggtt accgaagaat tccgtgccca aatcggagcg gatttttact
     7501 cacccaatcc ccaacaggcg gtcgagtatc tgaatcagtt tgtgagctga ttttcataac
     7561 gtttaattat tcaccttgtc cattctatta tctgtcagta ttcgatgggg gtataagtat
     7621 caatgggctt cgtgatctta ttcgtgattt ttcttaagtt cgtcgatttc ccagggcaag
     7681 ataatctggc cgggtgtgtt caattcggcg cgaatccgta taaaacataa tccagcgtat
     7741 tggtcttggg attgaatgga tttgccccat tccgtaaatg gggtttcgca agtagtcatt
     7801 ttacgactgg aagattcgcg aatgaacagt tttccatttt taactactga gataagcata
     7861 tgcgctgaaa aaatgtcgct tttgttggcg aaaattaaca ccagaatatc gccgttgcag
     7921 atattatttt cgactttaaa cagatattcc ttgggcacat attcgagggt aatagtacgg
     7981 tcaggcttga catagcgcaa atcggcaatg cctttatcct cgaaaaattt gcgatgtgaa
     8041 attgtgcgag tcgtttgttg aatgtattgt ccacccactt gatgtgagac gtcttccaat
     8101 aaccagctgt tttccggtaa ccaatcagcc attgtataat gattacgggt gcgcatgccg
     8161 ataatgccat cctgataccg aatttgctgg agattattga aaaaattgtc ccagctatca
     8221 gaaattgaga gcgccaatac atgttcacaa aaaaccatac aattagtttc tttgaaatta
     8281 actaatggcc agggttcata aagggcataa gggccatcgc cggcgcaatt ttggttgtat
     8341 ggcataccga gaaatttatc agagaagaga tttatgcgat cagtaattgt taggttctgg
     8401 gtgctgacaa ttgttaggag gctatcaatt tctggattgc tcatttgata aaacggcttt
     8461 ccctcaccca acaattcgga aataaataca ctgatgacca aagccagtga aattgtgata
     8521 tagatagctc ttttcataga tagctccata aaattgcttc aatagcggtc gaatttacag
     8581 cactgattac gaaatgcaaa tgatggcatg tttgctcttt aatcggcgag taatagaacg
     8641 gacaaaatag gaataaaaat tttaatccag cgaatggagc actaatccgc ttcttaattt
     8701 gggttcgaac ca
//
LOCUS       DAOOMU010000089         8442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_9858_length_8442_cov_21.743889, whole genome shotgun sequence.
ACCESSION   DAOOMU010000089 DAOOMU010000000
VERSION     DAOOMU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9858_length_8442_cov_21.743889"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLI55_08330"
     CDS             <1..327
                     /locus_tag="PLI55_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00876.1"
                     /translation="PHFSQTDLTKIRIVPNPYNINDPLLAAQGWTDQRGIQFYNLPGK
                     VTIKIFTENGDLVQVLKHDSPVEAGYYFWDMITLSQQVISSGVYIVVFQKPNGETAFQ
                     KFLVVR"
     gene            345..1361
                     /locus_tag="PLI55_08335"
     CDS             345..1361
                     /locus_tag="PLI55_08335"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPB00877.1"
                     /translation="MKKLIYLTITCILLLSLSSPINAQVGLKKVAQSTMNFLLVGVAP
                     RASAMGEAYCAFGTGAESIFFNPAGIAQSTHRFELQTYNTRWIADINYLAGSFIWNLH
                     QYGTLGLSMLSVDYGTIYGTSLLSAADKDLYPAGYIDLGNVDNVGSYAFGLTYGRAIS
                     TQFLIGGTVRLVGQNLGQSQMADGLKDNNAVKLAFDAGVKYLTDFKSFRFAMSIRNFS
                     SNIKREEMDEQLPLLFTMGVAMDLLDVIAPPFSQNNSLNLAVDFQHPNNYSERINYGL
                     EYLLFDQFAIRAGYQTNQDLASWSLGMGINVNIIGNTVQFDYSYSDFDLFDGVNRFSI
                     GIAL"
     gene            1358..3016
                     /locus_tag="PLI55_08340"
     CDS             1358..3016
                     /locus_tag="PLI55_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 43 family protein"
                     /protein_id="HPB00878.1"
                     /translation="MIIKKMMSSRHQRSCFHQRIFLIGFGLLTILAVNPLGAELPDLT
                     YSNPIIHTDYSDPDVIRVGDDFYLISSSFSQAPGVPVLHSRNLVNWQIIGYAVPHLPD
                     SIYDLPQPGRGIWAPALRYHNGEYWVYYGDPDLGIFMVKAQNPAGPWEPPVLVERAYG
                     WIDPCPFWDDDGQAYLIHAWAKSRVGFNSILTLHKLSSDGRQILDEGVTVFDGHSTHP
                     TIEGPKLYKRNGYYYIFAPAGGVTNGWQTGLRSKNILGPYEDKIVLEQGTTAINGPHQ
                     GAWIETNQGESWFIHFQDKGAYGRILHLQPVEWLNDWPLIGIDLDGNGIGEPVQCFRR
                     PDVDNPISAFQIQTSDEFESNSLGLQWQWRANFKSEWFTLSARSGYLRLFTVNPAPVC
                     YNLGDLPNILTQKFPAEAFTVTTKLSFHPDSNDDQAGLIIIGNDYAALKVTHRGARYQ
                     LTYAECTEIEKGKSEIISETVGLNKPEVFLKANVGEGAVCTFSYSTDGEKYLPLGRKF
                     QAVKGKWVGSQIGIFATTTPGKISSGYADFDFFRVMSIGKDEPK"
     gene            3013..4527
                     /locus_tag="PLI55_08345"
     CDS             3013..4527
                     /locus_tag="PLI55_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HPB00879.1"
                     /translation="MNIMKPKINFTLGRFLIFLTAAALTIIIYAAHNKPRVHLIGDST
                     MADKPLDDNPERGWGQLFPLYFTQEIQIINYARNGRSTKSFIDEGLWANVYNNLKPGD
                     FVFIQFGHNDAKFSDTTRYAPARTTYRANLVRFIEESRGKGAYPILITPVNRRKFNEA
                     GEFVDQHGDYPVVVRELAAEYDAPLIDLHARSRELFSQLGAEKSAELFLRAPAGVYRH
                     YPDGKSDNTHFNRYGAIRICELVIEEMKAIDLPLVKYLKDGLELSDMGVGKVVGLDYF
                     YNHEFKNGCQYHYTWEDRENSGFYELGNLFENCGAFIAEVCAEPTPEELARLSIYIIV
                     DPDTPQETTVPNYISEKAIKTITNWVESGGVLVLLGNDAGNAEFEHFNRLAQNFGIHF
                     NEVSLNRVTNKNYEMGKFDNLPDHPLFENITKIYMKEVASLTLVGNAEPILMSGGNVI
                     IASSKYGQGLVLAIGDPWLYNEYIDNRILPADFENYSAALNFCKWLLNQSRPVR"
     gene            4571..>6573
                     /locus_tag="PLI55_08350"
     CDS             4571..>6573
                     /locus_tag="PLI55_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HPB00880.1"
                     /translation="MKKRSCLELILTICILVTATVQAQTLAFVNWKCAPPDSQKVSAT
                     AGNLLGLPQIGAPGFVVRDYKEGPGPDQRWWPYENGSATYWGAESLQVDTRWVQFAAY
                     PKENITFQADSFTCFIGAKGTGEMRANVWYATDTSFADAIQINDSVLVLAKDNHIFYN
                     FAIDTTITSADTLFIRIYPWYQGSSSNSKYLYLLNATIWGTTEGEFVANPPTVTTAPV
                     TDISTTFAISGGTVTDDGGAPVTARGVCWKTTSMPDTSDNKTNDGESSGAFVSLITGL
                     EPGTTYYVRAYASNSAGIGYGEEVTFKTLDSLEVPTVETAKVTNIMVKTATCGGEVTA
                     WGGDTVRVRGVCWNTTGNPTITDSKTENGSGLGTFQSILYPLTENTTYYVRAYATNSK
                     GTGYGEVDSFTTQTPAPDVLKIVAADGSGDYPTVQAAFDDVPDYYTGRYIIYVKKGVY
                     HEKLFLDRYKMNVILIGEDRDSTILTYDDYAGKAGGTSNSYSVAIDADDFVAMNITFQ
                     NTVKNDGAFSDQQAVALRVNGDRQAYYNCSLLGYQDTYYLWGAWGNRVTGRVYMHKCY
                     IEGSVDFIFGRDIAVFDSCHINVNRNAGTITAASTEPGAKFGLVFRDCIITADEIGFN
                     ELPIGNIYLGRPWAAAPRTVFIKCEEPEVLNPGGWLEWNVVPAL"
     assembly_gap    6574..6673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6896..>8442
                     /locus_tag="PLI55_08355"
     CDS             6896..>8442
                     /locus_tag="PLI55_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00881.1"
                     /translation="MFRLDSQRRFIIKLPQNSQFINRGIIRKSWSTTPFFTSMILNWL
                     IIGQLLAQTSATSTWPLTANQNASVTGNLNAASQTLSNMQVKYESGVQRSSPSGIAGS
                     WPEETSENINRFMQFAVTPVEGYIFNVNSIEMKLYVNSGSNMRANVYYATDPLFVTKT
                     QIGSTYTLSSSVPSAPNITATLNYEVAYGDTFYVRIYPWYTTSTTGKYVITNSVVISG
                     TTIPSTYMLASPAALDGFIQENAAIPSSAQTYLLSGKNLIDSVTVDPPSNFEISADNG
                     STWISSGDSLRLPVLDGDIVGQPLSIAVHLKADSSGEYSGTIEHKSDATATFVNVSGA
                     FLANEPTVASVISADSVTGKTATLFFSGGNGMRRVITIRPGTEITWLPKDGKAVRGVS
                     SIFTEAIDQGDGTRIVYDGEDTTVTVTGLTGNTTYTAAIFEYNVATGNTYNYLTTQYG
                     IFTFTTAIVPELSVSPIVLNFGSVLISQKLIKSYTLTGRHLIENDVIHIIAPPDFDLS
                     LAPDTGFV"
BASE COUNT         2188 a         1973 c         1968 g         2213 t
ORIGIN      
        1 cctcactttt cgcaaaccga tttgactaaa atccgtattg ttcccaatcc ttacaatatc
       61 aacgatcctt tacttgccgc tcagggctgg actgaccagc gcggcattca gttctataat
      121 ttgcctggta aagttacaat caaaatcttt accgaaaatg gcgacctggt tcaggttctc
      181 aaacatgata gcccggtgga agccggttac tatttctggg atatgattac tctcagtcaa
      241 caggttatca gtagtggtgt ttatatcgtt gtgtttcaga aaccgaacgg cgaaacggct
      301 tttcagaaat ttctggtggt acgatgaagg cgcgaggaat tattatgaaa aaactgattt
      361 atttgactat tacttgtatt ttgctcctaa gcctttcctc gccaatcaat gctcaagtcg
      421 ggctgaagaa agtagcgcag agtacgatga actttttgct ggtgggtgtc gcaccgcgtg
      481 ccagcgctat gggcgaagcc tattgcgctt tcggaaccgg cgcggaaagc atttttttca
      541 atccagccgg tattgctcaa tctacccatc ggttcgaact gcaaacttac aatacacgct
      601 ggattgccga catcaattac cttgccggta gctttatctg gaatttgcac caatacggaa
      661 cgctgggatt gagtatgctt tcagttgatt acgggacaat ttatggtacc agcctcttgt
      721 cggcggcgga caaagacctc tatcctgccg gttatattga tttgggcaat gttgataatg
      781 tcggctccta tgcgtttggt ctaacttatg gtcgcgcaat cagcacccag tttttaatcg
      841 gcggcactgt acgtctcgtc ggtcaaaatt taggtcaaag tcaaatggct gacggcttga
      901 aagataataa tgcggttaaa ctggcgttcg atgccggggt aaaatacctg acagatttca
      961 agagctttcg gttcgctatg tccattcgca atttctcatc caatatcaaa agagaagaaa
     1021 tggatgaaca attaccgttg ctctttacga tgggggtcgc aatggatttg ctggatgtca
     1081 ttgcgccccc attctcgcaa aataattctc tcaatttagc ggttgatttt cagcatccga
     1141 ataactattc cgaacggatt aattatggac tggaatattt gctattcgat caatttgcga
     1201 tccgcgccgg gtaccaaacc aatcaagacc tggcttcttg gtcgcttgga atgggaatca
     1261 atgtaaacat aatcggcaat accgtccagt ttgattattc ctattctgac ttcgatttat
     1321 ttgatggcgt taatcgtttc tcaataggaa tagccttgtg ataattaaaa aaatgatgag
     1381 ttcacgtcat cagagaagtt gttttcacca gagaatcttt ctgataggct tcggattatt
     1441 gacgattctg gcagttaatc ccctcggcgc cgagttgccc gatttgacct attctaatcc
     1501 gataattcac accgactatt ccgatccgga cgttattcgg gtaggcgacg atttttattt
     1561 gatttcgtca tcgttttccc aggcgccagg tgttcccgtt ctgcattcgc gtaatctggt
     1621 aaactggcaa atcattggat atgctgtccc gcatttgccc gattcaatct atgatttacc
     1681 tcagcctgga aggggcatct gggcgccggc tcttcgttac cataacggcg agtactgggt
     1741 ctattacggt gatcccgatc tgggtatttt tatggtcaaa gcgcaaaatc cggctggccc
     1801 gtgggagccg ccagttctgg tcgaacgagc ttatggttgg atcgatcctt gtcctttttg
     1861 ggatgacgac ggtcaggctt atttgatcca cgcctgggca aaaagtcggg taggatttaa
     1921 cagtatcttg actttgcata aattgagctc cgacggtcgc cagattctgg acgaaggcgt
     1981 gaccgttttc gacggacatt ccactcaccc aacgatcgaa ggtcctaaac tttataagcg
     2041 taatggatat tactatattt ttgcgccagc cggtggcgta acgaacggct ggcagactgg
     2101 cttgcgttca aaaaatatct tgggtcccta cgaagacaaa atcgttctcg agcagggcac
     2161 tacagccatc aacgggccgc atcaaggggc ttggatcgaa acgaaccaag gcgaatcctg
     2221 gttcattcat tttcaggata aaggggctta cggccgcatc ttgcatcttc agcctgttga
     2281 atggctaaat gattggccgc taatcggtat tgatctggac ggcaatggga ttggtgaacc
     2341 ggttcaatgc ttccgccgac cggatgttga caacccaatc tctgcgttcc agatacaaac
     2401 cagcgatgaa ttcgagagta atagtctcgg gcttcaatgg cagtggcgcg cgaattttaa
     2461 atcggaatgg tttacactct cggctcgatc cggttatctg cgattattta ccgtgaatcc
     2521 ggcgcctgtt tgctataatc ttggcgattt acccaatatt ctaactcaaa aattccccgc
     2581 ggaagcgttc acggttacca cgaagttatc tttccatcct gatagtaacg acgatcaggc
     2641 tgggctgatt attatcggca acgactacgc cgcactcaaa gtgactcacc gcggtgcgag
     2701 ataccagctg acttacgccg aatgtaccga aatcgagaaa ggcaaatcgg aaataattag
     2761 cgaaaccgtt ggcctcaata agccggaagt attcctaaaa gctaacgtcg gcgaaggtgc
     2821 ggtttgcaca tttagctaca gtactgatgg cgaaaaatat ttaccgctcg gccgaaaatt
     2881 ccaagccgtc aaaggcaaat gggtcggctc tcaaattggt atttttgcta cgactacgcc
     2941 ggggaaaatt tccagtggct atgccgattt tgacttcttt cgcgtaatgt caataggcaa
     3001 agacgaaccg aaatgaacat tatgaagcca aagataaact ttacattggg caggttttta
     3061 atttttctaa ctgccgccgc tctgacgatc attatatatg ccgcgcataa taagcctagg
     3121 gttcacctga tcggcgattc gaccatggca gacaaaccgc tggatgataa tcccgaacgc
     3181 ggctggggac agctttttcc gctgtatttt acgcaggaga ttcagattat caactacgcc
     3241 cgtaacggcc gcagtacgaa gagttttatt gacgagggat tatgggctaa tgtttataat
     3301 aacctaaagc caggtgattt tgtttttatc caattcggtc ataatgacgc caagttcagc
     3361 gatacgactc gctacgctcc cgcccgaaca acctaccgcg ccaatctagt tcgattcatc
     3421 gaggaatcgc gcggcaaggg cgcctatccg atattgatta ctccagtcaa tcgccgcaaa
     3481 ttcaatgaag ccggcgaatt cgttgaccaa cacggtgatt atccggtcgt tgtccgcgaa
     3541 ttagccgctg aatatgacgc gcctctgatt gacttacacg cccgcagtcg ggaactattt
     3601 agccagctgg gtgcagaaaa atctgcggaa ttgtttctac gtgcaccggc cggtgtatat
     3661 agacattatc ccgatggaaa atccgacaac acgcatttca atcgctacgg cgcaatccgg
     3721 atttgcgaat tagttatcga ggaaatgaaa gccattgatc tgccgctggt aaagtatctc
     3781 aaagatggac tggaattatc ggatatgggc gtcgggaagg ttgtcggtct ggactacttt
     3841 tacaatcacg aattcaaaaa cggttgccag tatcactaca cctgggaaga tcgtgagaac
     3901 tccgggtttt acgaactggg taatttgttc gaaaattgcg gggcatttat tgccgaagta
     3961 tgcgctgaac cgacgccaga ggaactggct cgacttagca tttatatcat cgttgatccg
     4021 gatacgccgc aagaaaccac cgtgccgaat tatatttccg aaaaagcaat caagaccatc
     4081 acgaattggg tggaatccgg cggcgtatta gtcctgctcg gcaatgacgc tggcaatgcc
     4141 gaattcgaac actttaaccg gctggcacag aatttcggga ttcacttcaa tgaagtcagc
     4201 ctcaaccggg ttaccaataa gaattacgaa atgggcaaat tcgacaatct gccggatcat
     4261 ccgttatttg agaatattac caaaatttat atgaaagaag tcgcatcgtt gactttagtg
     4321 gggaatgccg aaccgattct gatgtctggc ggcaatgtaa tcatcgccag ttcgaagtac
     4381 ggtcagggat tggtattggc tattggtgac ccttggctat ataacgagta cattgacaat
     4441 cgcatactgc ccgctgattt cgagaattat tcagccgcct tgaacttttg taaatggtta
     4501 ttaaatcagt caagaccggt tcgctgattg aattggttat ttaaaattta tgattaaaca
     4561 ggaggtatgt atgaaaaaga ggagttgcct agaattaatt ctgactatct gcatactggt
     4621 caccgccacc gttcaagccc aaacgctggc gttcgtaaat tggaaatgtg cgccacctga
     4681 tagtcagaaa gtctccgcca cagctggcaa tctcttggga ctaccccaga tcggcgcgcc
     4741 gggctttgtc gtccgggatt ataaagaagg acccggaccc gatcaacgct ggtggcctta
     4801 tgaaaacgga tctgcgacct actggggagc agagagcctt caggttgata ctcgctgggt
     4861 acagtttgcg gcttacccca aagagaatat cacttttcag gcagacagtt tcacctgttt
     4921 cattggcgcc aaaggtaccg gtgaaatgcg tgctaatgtc tggtacgcaa ccgataccag
     4981 cttcgcggat gcaattcaaa taaatgacag tgtattggtc cttgccaagg ataatcatat
     5041 tttctacaac ttcgccattg ataccactat tacatccgcc gatactcttt tcattcgaat
     5101 ctatccctgg tatcagggtt catcttcaaa ctccaaatat ctttacttgc tcaatgctac
     5161 tatctggggc accaccgaag gcgaatttgt ggcgaatcct ccgacagtaa caacggcgcc
     5221 agtgactgat atttcgacaa cctttgctat cagtggcggt actgtcaccg atgacggcgg
     5281 cgcgccagtg accgcccgtg gcgtttgctg gaaaacgacc agcatgccgg acacttctga
     5341 caacaagacc aatgacgggg aaagcagtgg cgcttttgtc agcctcatca ccggattgga
     5401 acccggcacg acttattacg ttcgggcgta tgccagcaat agcgctggaa taggttatgg
     5461 cgaagaagtt acttttaaga cactggattc actagaagta cccacagttg agacggctaa
     5521 agtaaccaat ataatggtaa aaaccgccac ctgtggcgga gaagtaaccg cctggggcgg
     5581 tgataccgta cgcgtccgcg gcgtttgctg gaatacaacc ggcaatccta ctattaccga
     5641 cagcaaaacc gagaacggca gcgggctggg tactttccag agcattcttt atccgctgac
     5701 cgaaaataca acttattatg tgcgcgctta cgccactaat agtaaaggca ctggctatgg
     5761 agaagtggat tctttcacaa cgcaaactcc agctccggat gtgctgaaaa tcgtagccgc
     5821 ggacggcagt ggggattacc caaccgtgca ggcggccttt gatgatgtgc ccgattatta
     5881 caccggtcgt tacattattt atgttaaaaa aggtgtttat cacgaaaaat tgttcctgga
     5941 tcgctacaaa atgaacgtca ttctcatagg tgaagaccgc gacagcacca ttttgaccta
     6001 cgacgattac gccggtaaag ccggcggaac gtcgaattct tatagcgtcg ccattgatgc
     6061 cgatgatttc gtggcgatga atatcacctt tcagaacacg gtaaaaaacg atggcgcttt
     6121 cagcgatcag caggcggtgg ctttgagagt caacggcgac cgtcaggctt attacaattg
     6181 tagtctgttg ggttaccagg acacttatta tctctggggc gcctggggta atcgcgtcac
     6241 tggtcgtgtt tatatgcaca aatgctacat cgaaggctcg gtagatttca tcttcgggcg
     6301 cgatattgcc gtcttcgatt cctgccacat taatgtcaat cgcaatgccg gcacgataac
     6361 cgccgccagt accgaaccgg gagcgaaatt cggcttggtc ttcagagatt gcatcattac
     6421 cgccgacgaa atcgggttca atgaattgcc gattggcaat atctatctcg gtcgcccttg
     6481 ggcagcggca ccacgaacgg tttttattaa atgtgaagaa ccggaagtcc tgaatccggg
     6541 tggctggctg gaatggaatg ttgttccggc gctnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnttagcct gacagtgcat aatttgctgg gtcaaaaagt cctgactgtc
     6721 gttgatactt accagccggc cggatattat acggtaactc tggatggaag acagttatcc
     6781 agcgggttat atttctaccg gatcgagacg gaaaatttcc gccagactcg caaaatgatg
     6841 attttgaagt aatgaacggt tgattctata acattattat gactgaaata atttgatgtt
     6901 ccgattagat tcccagcgcc gatttattat taaactaccc caaaatagcc agtttattaa
     6961 tcggggcatt attcggaaaa gttggagtac aacaccgttt tttacttcaa tgattttaaa
     7021 ctggctcatc atcggtcaac tgctcgccca aaccagtgct acgagcacct ggccgctaac
     7081 cgccaatcaa aatgcctcag ttaccggaaa ccttaacgcc gcttcccaga ctttaagcaa
     7141 tatgcaagtc aagtacgaaa gcggtgtgca acgcagtagt ccgagtggaa tagccggttc
     7201 ttggccggag gaaaccagcg agaatatcaa ccgttttatg caattcgcag taacaccggt
     7261 cgaaggttat attttcaacg taaattccat tgaaatgaaa ctgtatgtca attctggaag
     7321 taatatgcgc gctaacgtgt attacgcgac cgacccgctc tttgtgacca agacccaaat
     7381 cggttcgacc tacaccttaa gttcatcggt accatcagcc ccaaatatca cggctacttt
     7441 aaactacgaa gttgcttacg gtgatacttt ctatgtccgc atttatccgt ggtacaccac
     7501 ctcgacaacc ggcaagtatg tcatcaccaa ttcggtggtt atatcgggta caactatacc
     7561 atctacttac atgctggctt caccggcggc gctggacgga tttatccaag aaaatgccgc
     7621 aattccctca tcagcccaga cctatttgct aagcggtaaa aacctaatag attccgtaac
     7681 agttgatcct ccatcgaact tcgagatctc agcggacaac ggctcgacct ggattagtag
     7741 cggcgactcg ctccgcctgc ccgttttaga tggcgatatc gtcggtcaac ctctttctat
     7801 cgccgttcat ctgaaagccg actcatccgg cgagtatagc ggaacaatcg aacataaaag
     7861 cgacgccaca gctactttcg tgaacgtatc gggagccttt ctggcaaatg aaccgaccgt
     7921 agcttcggta atatcagctg actcggtaac cggtaaaacg gctactctat ttttcagcgg
     7981 cggtaatggt atgcggcggg tgatcacaat tcgccccgga actgagatca cctggttacc
     8041 gaaagatggt aaggcggtca ggggcgtttc gtcaattttt actgaggcaa ttgatcaagg
     8101 cgatggcacc agaatagttt atgacggcga ggatactacc gttactgtta ccggcttgac
     8161 cggcaatacg acttataccg cggcgatttt cgaatacaac gtcgcaaccg gaaatactta
     8221 taactacctc acaactcaat atggcatatt tacctttaca acggcaatcg tacctgaact
     8281 tagcgtatca ccgattgtgt tgaatttcgg cagtgtgctt atttcccaga aattgatcaa
     8341 aagctacaca ctcacaggcc gtcacttgat tgaaaatgac gtgatccata ttattgctcc
     8401 accggatttt gatttatcgt tagcgcccga tacgggattt gt
//
LOCUS       DAOOMU010000090         8426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_9881_length_8426_cov_8.100108, whole genome shotgun sequence.
ACCESSION   DAOOMU010000090 DAOOMU010000000
VERSION     DAOOMU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8426
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9881_length_8426_cov_8.100108"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2276
                     /locus_tag="PLI55_08360"
     CDS             <1..2276
                     /locus_tag="PLI55_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB00882.1"
                     /translation="FAQGSYNTQDRTYPGQNFKVWGSVSNRFFNDKLGIFIQGNADRS
                     NVGNDETTAGYDIDNSAAPYGEGEYQMLNFVFLDEENIITNYGGSVFLDYKLPNGKII
                     LQNALAHTLNDNAKYRLEYDLSVTEVGYALNRDKHNKELMINALQVSYNFGKVKADLG
                     LSHSFSDKATDIRYGDPGNEFGFQNVASDTTFIDNNGAPITYEGARRELTPEDVFNIK
                     LKSDNWENAPIYGWCVTRNEAFKQHLYNGTLDFTVPVSLLRAISGDIKLGGKFTRSVR
                     TNDVEAAYHRTGDDDFYNGVLDFLGPDDSLHVKKRPLYFSDVRNYDYDRGKYFFEGDY
                     EFLYAMDKDMMDEFIPNAVSGWIKSRHEGNSLRDDFDGSELFTAAYIMGDFNIGPRLS
                     LIGGGRFEHFNMDYTANFVYVTHSVDGDANEKTRTIERNDDTFFPNAQIRYKFTEWGD
                     IRAAYSKTISRPDYIAIKPNVYYENGGAVSAGNSALKPSIANNIDVYMSLYNNQIGLF
                     TIGGFYKKIDDVFFQTKIYYPFLSYYGVSCPDSAGMVGAGLNIIPSQVPGRSTEVTTY
                     VNNPNAAYIKGFELDWQTYFYYLPKPFNTLVLSANYTKTWSEMDYLQLELKDSTYKDG
                     KIIKHIYKMVENKRNMRLLGQADDVINVALGVNHKGFAGRISYRYTGEMLTWIGVRPE
                     EDEYIQPISRWDISLKQKLPIKGLVIGFEGINIFHSPEKTYRVFRRVANGPIFENEVT
                     TRYIPRKFSLYLRYNFE"
     gene            2415..4670
                     /locus_tag="PLI55_08365"
     CDS             2415..4670
                     /locus_tag="PLI55_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HPB00883.1"
                     /translation="MKKGLLLSVVVCLLLFSAEFALGALGDYVSEVRGDTLVIKDYAD
                     FEPYAAGSLREAIIADSVDVPAGRVYELKANGYYPLSSNLTTPASRAVVIVGADDTRL
                     VKNANEDSAPPVICGFGSNSGGINFANDLTVKNCNIIQAASRAQLGWAFFGAAASNKT
                     LRVENCLFEHTRWVMIQSNDADGTSVYINDCYFVNMSGQACRRNGGVYDNVGRNTNTM
                     WVENSTHVMAQGMIYKFRNFQVGKIVFNHNTFVNMSGQVFETLGYQSDMVVVNNIFVN
                     CNLQPYMTGLDYGETDADYLPMGIINVDTLPAGYTDMPRKVLVDRNVVYWDPRFADMV
                     QTCKDNMVNQNDDWHDQMITMNTRTQAMFDDDETYPYLVEGAWLRKCPTFTDSKDLLT
                     VGVDSIKAFSVGTVDTLSGDVLMVWRKVYTNLDDYYVYSDWPIPVDLSYTDKDLLAAA
                     YGRFPVGDLNWFPDKKAAWAAQREAEIAAIEELRNSGKIDIATNGGFEQTPAGSVTNL
                     AKGITGWVIEVAAGIDPAPAFEIVTDPVQEGDQALAITVNALGTNAWDIQVVAEDIPV
                     KPGATYTYSIWAKASAAAKANFTVGNADYNEYTRVHEKALTTEWTEIRKEFTVNDQYT
                     VIRAPIHVNIAGNEGITIYIDNLTITEKPSAIAPKTTPEKFALRQNYPNPFNPTTTIN
                     FTLPEPGNVTLKVYNMLGQEVATLINNEHRGANATHSILFDGSKLSSGVYFYTLTYGD
                     KTVSKKMVLMK"
     gene            4900..>5927
                     /locus_tag="PLI55_08370"
     CDS             4900..>5927
                     /locus_tag="PLI55_08370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB00884.1"
                     /translation="MKSNFYLLILLISMTANIFTNCAKQTELAQKTVAVIDDHYKISF
                     ADLHKYVYDEFFHRRYRDMSEAYRRALNEMVLDQLKCIDFFKRGLHNDPQLTQELQRY
                     INEELIVKYFELEYLGKYTSEEFARKMYDQMGKEVHYQQIVLNKPKNVSDDQLKSLEA
                     QAKEIKAQADKGINFKELIKQYSETELENIDHSFSIMGWRESLENPHNRVIFNLNKGE
                     IRILDEYHAYYIIKITDVKKINLEPFKKIKNELISKLSESYYYTSLNEYDRDKEALIN
                     EDSIVWNEKALDQLVKWSRIPNFYKAIYRDTLQNAISSGRNLVILTYPEGQVDLKEYL
                     RLLNEILT"
     assembly_gap    5928..6027
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6157..>8426
                     /locus_tag="PLI55_08375"
     CDS             6157..>8426
                     /locus_tag="PLI55_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00885.1"
                     /translation="MNYKKLAYLNLFLVSATVICFEIIATRISSVIFVSNYAFIILSL
                     AIFGLGMGGVFSYYRFNSSKIPQVIRTIACFIFLTGISLLLFIISVIGFSITNPFIYF
                     LLLILPFFFAGVVYALIFKIFADESFKLYASDLIGAAVGSGVALLIFNIFNAPNAVLF
                     LSLIMFLSAAIFAHEFFKKKMAIGIYTLIFLGMIILSCFGHKEFLGEVPIGKFPEKDF
                     YYVYPNLTPFDYKIIDSRWSLYGRSDLVEYRQQDMVKQLFIDGAAGSPMYRFNGNLQN
                     PDVLLLNLLINQSTTIPFMFLQGNARDNMLVIGPGGGKEVLIGLLTGIRQITGVEVNP
                     DFVDIVKGQSFFNGGIYTGFPNVRILVKEGRHFIKSTNQKYDLIVMALPSTEQLQNID
                     HLAASENYLLTVEAIQDYLKILTPEGSLIFTVHNRWELIRLIVNTLYAMEDIGISNQE
                     ALNHFIILAEDYAPTIVIKKNAYRPDDMAVINNIIEQLPPELPHITYRPYHWNQINNT
                     IENQLLRNIRDNRISLQQYIEQDEFDISPVYDDSPYFYKISRGIQRDYLWLLIGVAFF
                     SIILITVPLIKIKKSISKNGMHSLIFPLKIFILIGIGFMIIEVTLFQKLILYLGSPTI
                     SLSVLLSSLLIGMGIGSYFGKRIQPENPPKRLKVVSLAIVIVGVFTILFCPYLLNKLL
                     KYSQVLRLIVGFVLLLPCGFLLGIPFPSALELLKLSNQEKYIPWMYGVNGSMSVLGSV
                     LAVILSMVCGYTLTFY"
BASE COUNT         2443 a         1523 c         1785 g         2575 t
ORIGIN      
        1 tatttgccca gggtagctat aatacccagg atcgaactta cccaggccag aatttcaaag
       61 tatggggcag tgttagcaac cgttttttca atgataagct gggaatattt atacagggca
      121 atgcagaccg gtctaatgtt ggaaatgatg aaaccactgc cggttatgac atcgataatt
      181 ctgcggctcc gtacggcgag ggtgagtatc aaatgctaaa ttttgtgttt cttgatgagg
      241 aaaatataat taccaattac ggtggtagtg tatttctgga ttataaactc cccaatggta
      301 aaataattct gcaaaatgca ttagcccaca ccctaaatga taatgccaaa taccgactgg
      361 aatatgatct ttctgtaact gaagttgggt acgccctcaa tcgcgataag cataataagg
      421 aattgatgat taatgctctg caggtaagct ataattttgg taaggtaaaa gccgatctgg
      481 ggctctctca ttccttcagt gataaagcta ccgatattcg ttatggagat cctgggaatg
      541 aatttggttt tcagaatgtc gcatccgata ctaccttcat cgataacaat ggcgctccca
      601 ttacctatga gggagccagg cgggaactta caccagaaga tgtatttaat ataaagctca
      661 aatcagacaa ttgggagaat gcgcccatat atggctggtg tgttacccgt aacgaggcat
      721 ttaaacagca cttatacaat ggcaccctgg attttaccgt tcccgtatct cttttaagag
      781 cgatatcggg tgatattaaa cttggtggca aatttactcg ctcggtgcgg accaacgatg
      841 tggaagcggc ttatcaccga accggtgatg atgattttta caacggagtt cttgactttt
      901 taggccctga cgatagctta catgttaaaa agcgcccatt gtacttctcc gacgtaagaa
      961 actacgatta tgatagaggc aaatatttct ttgaaggtga ttacgaattc ctctatgcca
     1021 tggataaaga tatgatggat gagttcatac ccaatgcagt ttcagggtgg ataaaatccc
     1081 gacatgaagg caattcactg cgagatgatt ttgatggctc ggaacttttt actgcggctt
     1141 acataatggg tgattttaat attggcccgc gtttatcgtt aatcggtggc ggtcgattcg
     1201 agcacttcaa tatggattat acagcaaatt ttgtctatgt aactcattca gtagatggcg
     1261 atgccaatga aaagaccaga actattgagc gcaatgacga tacctttttc ccgaatgccc
     1321 agattcgtta taagttcacg gaatggggag atatccgggc ggcttattcc aagactattt
     1381 cgcgacctga ttatatagcc attaaaccga atgtctatta tgaaaacgga ggtgccgtat
     1441 ctgctgggaa ttcagctcta aaaccatcta tcgcgaataa tattgatgtg tacatgtcac
     1501 tatataataa tcagattggt ttatttacca ttggtggctt ttataagaaa attgatgatg
     1561 ttttctttca gaccaaaata tattacccat tcttaagtta ttatggtgta tcttgcccgg
     1621 attcagccgg aatggttgga gccggactga atattatccc ctcacaggta cctggaagaa
     1681 gtacagaagt gacaacttat gtcaataatc ctaatgcggc ttatatcaaa ggtttcgaac
     1741 ttgattggca gacctatttc tattatttac caaaaccatt taatacactg gttttgagcg
     1801 cgaattatac caaaacctgg tcggaaatgg attatctgca acttgaactt aaagattcaa
     1861 cctacaagga cggaaaaatt atcaagcata tttataaaat ggtcgagaat aaacgcaata
     1921 tgcgacttct cggtcaggct gatgatgtca taaatgttgc gttgggagtg aatcataaag
     1981 gttttgcggg gcgtatctcc tatcgatata ccggcgagat gcttacatgg attggagtaa
     2041 gaccggaaga agatgaatat atccagccta tttccagatg ggatatatca ttaaaacaga
     2101 aattacctat caaaggtttg gtgattggtt ttgaaggtat caatatattt cactcaccgg
     2161 aaaagaccta ccgggtattt cgcagggtag ctaacggtcc gattttcgag aatgaagtca
     2221 cgactcgata tatcccgcgc aaatttagcc tttacctgag gtataatttt gaataaaagg
     2281 aatcattttt tgctactaaa ccaaataaac gaaaggaggt tggatggttt aaaaaattgc
     2341 gttaaggctg tttgtactaa cagctcgtta ttttcattgt tggtagtatt aatttaacta
     2401 aaaggagatt tgttatgaaa aaaggattgt tattatcagt ggtagtctgc ctgttgctat
     2461 tctccgctga attcgccctt ggagctctgg gtgactatgt ttccgaggta agaggagaca
     2521 ccctggtaat taaggattat gccgattttg aaccgtatgc ggcaggctct ctaagggaag
     2581 ctataattgc tgattctgta gatgtacccg cgggtcgtgt ctatgagttg aaagctaatg
     2641 gatattatcc gctttcgtct aatctgacca cccctgcgag tcgggctgtg gtaatagttg
     2701 gcgccgatga tactcgttta gtgaaaaatg ctaatgaaga ctcagcgcct ccggtaatct
     2761 gcggatttgg ttccaattca ggcggcatta attttgcgaa tgatttgacg gtgaaaaatt
     2821 gcaatattat tcaagccgcc agtagagcgc agttaggctg ggcatttttt ggtgcggccg
     2881 cgtccaataa gacgcttcgg gtcgaaaact gcctgtttga acataccaga tgggtcatga
     2941 ttcaatccaa tgacgccgat ggaaccagtg tatatattaa cgattgctat tttgttaata
     3001 tgagtggtca ggcttgccgg cggaatggtg gtgtctatga taatgtcggt cgtaatacca
     3061 acactatgtg ggttgaaaac agcacgcatg tcatggctca gggcatgatc tataagttcc
     3121 gcaacttcca ggttggcaaa atcgttttca accataatac ctttgtcaat atgtcaggcc
     3181 aggtttttga aactctcgga tatcagagtg acatggtcgt agttaataat atttttgtta
     3241 actgcaatct acagccttat atgactggtt tggattatgg tgagaccgac gccgattatc
     3301 tgccgatggg tattattaat gttgacaccc tgccagctgg ttatacggat atgcccagaa
     3361 aagtgctagt tgatcgtaat gttgtctact gggatcccag attcgcggat atggttcaga
     3421 cctgcaaaga caatatggtc aatcaaaacg atgattggca tgatcagatg attacgatga
     3481 ataccagaac tcaggcgatg tttgacgatg atgaaactta tccttacctg gtcgaaggcg
     3541 cctggttaag aaaatgtccc acttttaccg attctaagga tctgctgacc gttggagtcg
     3601 attcaatcaa agcctttagc gtaggtacgg tagacactct cagtggcgat gtactgatgg
     3661 tctggagaaa agtatatacg aatctggatg attattatgt atattccgat tggccaattc
     3721 cagttgatct ttcttatacc gataaagacc tcttggcagc cgcttatggc cgatttccag
     3781 ttggtgactt gaactggttc ccggataaaa aagccgcgtg ggcagctcaa cgagaggctg
     3841 aaattgctgc tatcgaagaa ttaagaaata gcggtaagat tgacatcgcc actaatggtg
     3901 gttttgagca gacaccggct ggttctgtga ccaatttagc caaaggaatt accggctggg
     3961 ttattgaagt agccgctggt attgatccgg caccggcttt tgagattgtt accgacccag
     4021 tacaggaagg tgatcaagct ctcgctatta ccgtcaatgc ccttggaact aatgcctggg
     4081 atattcaggt agttgctgaa gatattccgg taaaacctgg tgcaacttat acctattcaa
     4141 tatgggcgaa agcctcagcc gcggctaagg ctaatttcac agttggtaac gctgattaca
     4201 atgaatatac gagagttcat gaaaaagctc taactacaga atggacagaa attcgcaaag
     4261 agtttaccgt caacgatcag tacaccgtaa tcagggcacc tatccatgtc aatattgccg
     4321 gaaatgaagg cattactatt tacattgata acctgaccat caccgaaaaa ccttctgcta
     4381 ttgcgccgaa aacgactccg gaaaaattcg ctttacgtca gaattatccg aatccgttta
     4441 acccgacgac gactataaac ttcactcttc cggaaccggg caatgttact ctgaaagttt
     4501 ataatatgtt gggtcaggaa gttgccacat taattaataa tgaacaccgc ggagctaatg
     4561 caactcatag tattttattt gatggttcca aactttccag cggagtatat ttctataccc
     4621 ttacctatgg tgacaaaaca gtatccaaaa agatggtatt aatgaagtag acatttctat
     4681 tattcaatta atgaatgact tgtgcaaaac cccggtatat tttgccgggg ttttgcataa
     4741 aaaaacaatg gtactcaaat tcattagtga cataaagaat ataaaaactc ctattttatt
     4801 ggaactaatt attactactc gcccgttaat tgtcgtgaat ttaccagtca gtttgttaaa
     4861 ttttaatcca gaaaatcttc caattaaatg aggtttatga tgaaatcaaa tttttactta
     4921 ttgattctgc ttatttcgat gacggctaat attttcacca attgtgctaa acaaaccgaa
     4981 ctggctcaga aaaccgtcgc ggtaattgac gaccattata aaatcagttt cgccgattta
     5041 cataagtacg tctatgatga attttttcat agaagatatc gcgatatgtc cgaagcctac
     5101 aggcgagcgc taaacgaaat ggtattggat cagctgaagt gtattgactt ttttaaaaga
     5161 ggtttgcata atgatccgca attaacacaa gaacttcagc gctatattaa tgaagaatta
     5221 atcgtcaaat atttcgaatt ggaatatctg ggtaaataca ccagtgaaga gtttgcccgg
     5281 aaaatgtacg atcagatggg aaaagaggtc cattaccaac agattgttct gaataaaccc
     5341 aaaaacgtct cagacgacca attgaaatca ttggaggctc aagccaaaga aataaaagcg
     5401 caagccgata aaggcattaa tttcaaagaa ttaattaaac aatattcgga aacagaattg
     5461 gaaaatattg atcattcttt ttctataatg ggctggaggg aaagtctgga aaatccgcat
     5521 aacagggtga tttttaatct taataaaggt gaaattcgga ttctggatga atatcatgcc
     5581 tactatatta tcaagataac cgatgttaag aaaatcaacc ttgagccttt taagaaaata
     5641 aagaacgaat tgatatctaa acttagtgaa agttactact ataccagttt gaacgaatat
     5701 gatcgagaca aagaagcact aatcaatgag gattctatag tatggaatga aaaagcactt
     5761 gatcagctgg tcaagtggtc gagaattccc aacttctata aggctattta tcgggacact
     5821 ttacaaaatg cgatttcgag cggtagaaat ttagtgatct taacttatcc ggaaggtcag
     5881 gtggacctca aggaatatct gcgactttta aacgaaatat taactttnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnntct tcttcaaaaa agaaaaaatg agaagagttt
     6061 gcaaaataat acaaaataaa gcgtgaagat attttttaca gttacttgac ttaattgggg
     6121 tatgtattta gattccgaag tgggttagcc tgatttatga actataagaa attagcgtat
     6181 cttaatttat ttctcgtttc ggctacggtt atttgttttg agattattgc tacccgaata
     6241 tcatcagtga tttttgttag taattatgct tttataattt tatcgcttgc cattttcgga
     6301 ttagggatgg gcggcgtttt ttcatactat agattcaatt cctctaaaat tccccaggta
     6361 atccgaacga tcgcctgctt catttttctc acgggaattt ccttacttct atttataatc
     6421 agcgttatcg gcttctccat cactaatcct tttatctatt tcctgcttct tattttgcct
     6481 tttttctttg ccggggtcgt ttatgcccta atttttaaaa tattcgccga tgagagtttc
     6541 aagctttatg catccgacct gattggagcc gctgtgggct cgggtgtagc actcctgatt
     6601 tttaatattt ttaatgcgcc taatgcggtt ttatttctgt ctcttattat gtttttgtca
     6661 gccgctattt ttgcccacga atttttcaaa aagaaaatgg caatcggtat ttatacgcta
     6721 atctttttag ggatgataat cctaagctgt tttgggcata aggaattttt aggggaagtg
     6781 ccgatcggaa agttccctga gaaggatttt tattatgtct atcctaatct cactccattt
     6841 gattataaaa tcatagatag ccggtggagt ttgtatggtc gttcggatct ggtggagtac
     6901 cgccagcaag atatggtcaa gcagttgttt attgacggtg cggctggttc accgatgtat
     6961 cgttttaatg gtaatctcca gaatccggat gttttgctgt tgaacctttt aatcaatcag
     7021 tcaaccacga ttccctttat gtttctccag gggaacgcga gggataatat gctggtaatt
     7081 ggtcccggcg ggggtaagga agtattaatt ggtttgttga cgggaatcag gcagattacc
     7141 ggagtggaag tgaacccgga cttcgttgat attgtgaagg gtcagagttt ttttaacggt
     7201 ggaatttaca ctggtttccc caatgttcga attttagtta aggaaggtcg tcatttcatc
     7261 aaaagcacca atcaaaaata tgacctaatc gtgatggctt tgccttcaac cgaacagctg
     7321 cagaatattg atcaccttgc cgctagtgag aattatcttc tgacggtcga agctattcaa
     7381 gattatctga agatattaac tcccgagggc agtctgattt ttaccgtgca taaccgttgg
     7441 gaattgatcc ggcttatcgt gaacacgctt tatgctatgg aagatattgg aattagcaat
     7501 caagaggcgt tgaatcactt cattatcttg gcggaggatt atgcgccaac gatagttatc
     7561 aaaaaaaatg cctatagacc ggatgatatg gctgtcatta ataatataat cgaacaattg
     7621 ccgccggaac tgccgcatat cacctatcga ccctatcatt ggaatcagat caataatacc
     7681 attgagaatc aattattgag aaatatcagg gataatcgga tttctctaca acaatatatc
     7741 gagcaggatg aatttgatat ttctccggtt tatgacgata gtccatattt ttacaaaata
     7801 agtcggggga ttcagcggga ttatttatgg cttttgattg gagtggcatt ttttagcatc
     7861 attttgatta ctgtgcctct aattaaaata aaaaaatcca ttagtaaaaa tggaatgcat
     7921 tctcttattt tcccgttgaa aatctttatt ttgattggta tcggatttat gataatcgag
     7981 gtaactcttt ttcaaaaatt aatcctatat ctgggttcgc cgacgatttc attgtcagta
     8041 cttttatcct cgttattaat cggtatggga atcggtagtt acttcgggaa aagaatccaa
     8101 ccggaaaatc ccccaaagcg gctgaaagtt gtcagcctgg caattgtcat agtaggagtt
     8161 ttcactattt tattttgtcc ttatttgctg aacaaactat taaaatatag ccaggttttg
     8221 cgtttgattg tgggctttgt cttattactg ccctgtggtt ttttgctcgg aattccattc
     8281 ccttcggcgc tggaattatt aaagctctcg aatcaggaga aatatattcc ctggatgtac
     8341 ggagtgaatg gtagtatgtc ggttcttggt tccgtgctgg cggtaatttt atcaatggtt
     8401 tgcggttaca ctttaacctt ttatat
//
LOCUS       DAOOMU010000091         8376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_9963_length_8376_cov_19.471097, whole genome shotgun sequence.
ACCESSION   DAOOMU010000091 DAOOMU010000000
VERSION     DAOOMU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9963_length_8376_cov_19.471097"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..2280)
                     /locus_tag="PLI55_08380"
     CDS             complement(82..2280)
                     /locus_tag="PLI55_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00886.1"
                     /translation="MRRDYVKFAIMPAVMGAVAMIAQVAYLRLTAQSFYGNELTMCIA
                     LGHWLIWTGLGSLVGARLVRRNLSEKWLTAIVLSYGMVLIIFAGLLFLNRRIAGILAG
                     TVVGLGTIFAWTFILFSLPAWLNGLFFPLIVNWVKVKAELSPVHTVYAGEVIGSALGS
                     LLFAGLILSGVSTIDCLLVIVWVMMVLAIGFFIYSERMRIFLIIGASLIVILASVGFR
                     GPITNWRWAPFRALLSRESPHLALTTVRYQDAISVYGDSQPLWTFGDREKAEEWTHFA
                     LLNHPDPRSVLVIGIGSIEICREISRHPSVERITILQPDRILHQMVTQFGSIDALDTN
                     VQVIFADPALYLGKTDERFDVVLMNIPLPVNAQWNRFYTREFFEIVHSRLSPGGLLML
                     NLPGDEEFLPPAQVEFLKTMQNTANRVFQETTWIPGLTVHLLASDSTLHNDRAFFLDG
                     LRSRNLEPIYISDYFLHDRLSNWKIDFLTNRISEAEIQEVNTIAKPIGYYYDTVLWGQ
                     RTGGIVKKVYPFLRELGFIPILIGFIVVILIVRPISHRKKIDPAVRMAGIGFWVMGLE
                     SVAIILFQSLVGSVYLWIVFLTFAYMLGSGLGAIMQLKWKRADIILERVGFLLMLILP
                     FLLALPLTGGWPILLVTLWIGLILFIGGVVAGCIFPLMVKASTVQADKIANSAGLIYA
                     ADIFGSTLGTYLISGIVIPVYGIIPALGIVVAVELGIGAFNFPVTHLTEK"
     gene            complement(2327..3160)
                     /locus_tag="PLI55_08385"
     CDS             complement(2327..3160)
                     /locus_tag="PLI55_08385"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HPB00887.1"
                     /translation="MKKQTRKLTILFLCFLIWAVGLFSDAPEPVPKTSDVAQNVVRIG
                     VLKLPAVLVKGGTFEMGSDDESSPEDTRPAHTVTLTDFYISVTEVTVGQYKEYCRQTG
                     KAMPEQEAGTNDSYPIAFVTWYEANEFCQWVGGYLPTEAQWEYAARSRGLNLKYPNGN
                     TIDHSFANYSGTGQTDKWKRVAPVAKFPPNSLGLYDMAGNLYEWCQDYYRSDYYKNSS
                     QINPTGPASGMFKVIRGGSWYHGKEAMRTTSRYRYMPVARLSFLGFRVAWDPDAVRVS
                     E"
     gene            3385..5913
                     /gene="pulA"
                     /locus_tag="PLI55_08390"
     CDS             3385..5913
                     /gene="pulA"
                     /locus_tag="PLI55_08390"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HPB00888.1"
                     /translation="MKNYLNNLKTGLIIFGLIASMITSIPKGANEMQTLTVHYHRYDG
                     AYQNWTLWTWSENFSTEIKATGQDDYGLIFNLDLSIYPNPKSIGLLPKYGSWERKDDP
                     DRYWKPNSHHEVWLLQGDETVYNSRPDISPKIRRAFLDSPTTITLVFSNAITKEMSTS
                     LAPTIKLKDGQTIQPGRICLIPENSHSSEIVRLLDLPAIEIDNLPATVSIPNFADCPV
                     WLRYIRDDEKFITQEPLGAFYSPDSTKFAIFAPGATAVVLHIYDQPQGGKPTKYKLTK
                     IQNSLWSVTVKGDLKGKYYVYSVAGPDPAYEPKKEVLDPNTTCATTHDGRALIFEENT
                     QIAVAPKFLLKDAIIYEMHVRDFTIAENSGVKNKGKYLGFTEEGTTIPGTSISTGLDH
                     LTELGINTIQLLPIQDFEHDNKVNNYFWGYMTVNFNSPDGWYTTNENDESRVREVKQM
                     VSALHKRGIKVVMDVVYNHTAETNPQIRYNFNGIVPSFYYRQREDGSYWNGSGCGNEV
                     RSENPMVRRFIIESLKYWVKTYDIDGFRFDLMGLIGIETMRQIVKELTALKPDIFLYG
                     EPWIVGETPIKPILKGAQREEGFAVFNDNFRDALKGPWHNTDPGYVQTGKNIEAVKIG
                     IRGSIDDFAADPIETINYVSCHDGRTLWDQLVASTGNNKMYTDTQLKAMDKLAAFIIF
                     TSQGIPFMQGGEEFLRTKFGSNNSYNQPDKINKIRWDYKQENHDIFAYYQGLIRIRRD
                     HPMFRMETAEEVRRNLAFFEELGLSVPPNCIAYRLQRGTSGDIWREVIILINPNSRPE
                     TFKIPKAKWILAVDHTYADPEIISPISGPLVTVKPISAMLIYRL"
     gene            5942..8350
                     /locus_tag="PLI55_08395"
     CDS             5942..8350
                     /locus_tag="PLI55_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPB00889.1"
                     /translation="MKTTRFHSCLRLIVLPLLICGLFNCTQRNVLNKPPEIIEFVRID
                     SGCSTSLSLSEIFTAKKYKPIFSPNPDIQLVYQPDSNIIILTPNNSFSGLTMINFTNQ
                     KKDLCLPIIVKQKNLVTFSYKPPKPVREIFVMGNFNDWNRSSLPMSDEDSDGIFTRDV
                     WLDDGLYEYQFVVDQNEIYDPANPEKTDNGFGYFNSVLRVTAPLADYAANLYILPTRQ
                     NDTITLAVDCLGDTSDIRYFVLLDNHLCPPKSYHHKNKQLQIYLNSLENTIGMHILRA
                     TAQYKNQPGNVITIYLKNGKPLDNNTFIWNDACLYAIMTDRFKNGNSGNDRPVVHPHL
                     ASQANFKGGDFDGIRRTIESGYFDDLGINTLWISPVNKTTDSAYQEWPEPHNYFSGYH
                     GYWPTDARQTDSRFGSLQELKKLVEVAHRHNIKILLDFVSNHVHIEHPYFREHREWFG
                     QLELPDGSLNIRRWDEYRLTTWFDTFLPSFDYEGSHEALQTMTDNAVWWLKQTDADGF
                     RHDATKHVPHAFWQTLTSKINKRINPHRNLDIFQIGECFGSNELIKSYVNNGMLESQF
                     NFNQFFTARRVFVEPTGDFRDLELAIKKALEVYGYNHLMGNLMDSHDQVRIMAFLDGD
                     LTLSDNGTMRAWQEPPITVDNPLTYRKELVFLGYILTVPGIPVIDYGDEFGLTGANDP
                     DNRRMMRFGEELSDLEREQLQQVSNLIQLRNMHPALRRGDYLPLKAEKDIFIYSRGDA
                     NERLIIALNKSPDIQNPIIFLPDWLRGQSLRSLTNNNTWPIDYNTVKVSLAGYSVDIF
                     TINP"
BASE COUNT         2495 a         2015 c         1669 g         2197 t
ORIGIN      
        1 tttctcggct caggtattca accgtgagac ggtgaagtaa caaacaatta attagagact
       61 tatcactcag cctttatccg gctatttttc ggtcaggtga gtaaccggaa aattgaatgc
      121 gcctattccc aattctaccg ccaccacaat tcccagcgcc ggtataattc catatactgg
      181 aatgacaatt ccggaaatca aataagttcc gagtgtcgag ccaaaaatat cggcggcata
      241 gatcagaccg gcagaattgg ctattttatc tgcttgtacc gtgctggctt ttaccatcaa
      301 tggaaatata cagccagcca caactccacc gataaataaa attagtccaa tccagagcgt
      361 caccagcaaa atcggccatc caccggttag cggcaacgcc aggagaaacg gtaaaattag
      421 catcagtaaa aagccaaccc gctcgagaat aatatctgct ctcttccatt ttaattgcat
      481 aattgcgccc agaccagaac ccagcatata agcaaaagtc aaaaatacga tccacaaata
      541 aacactaccc actaaactct gaaaaaggat aatggcaacc gattccaagc ccatcaccca
      601 gaaaccaatc cccgccattc gcaccgccgg gtctattttt tttcgatggg aaatcggtct
      661 aacgattaaa atcaccacta taaaaccaat cagaatcggg ataaagccca gttcccgcaa
      721 aaacggatat acttttttaa cgatcccgcc ggtgcgttga ccccaaagca cggtatcata
      781 ataatagcca atcggtttgg cgattgtgtt cacctcttga atttccgcct ccgaaattcg
      841 attggtcaga aaatctattt tccagtttga gagccgatca tgtagaaaat aatcgctaat
      901 atagattggt tcgaggttac ggcttcgcag accatccaga aaaaacgctc gatcgttatg
      961 tagagttgaa tcgctcgcca ggaggtgaac tgtcagaccg ggaatccagg ttgtttcctg
     1021 gaaaacccgg ttggcagtat tttgcatagt tttgagaaat tcgacttgag ccggtggcaa
     1081 aaattcctca tcaccaggta aatttagcat caaaagcccg cccggactta atcggctatg
     1141 tacgatttcg aaaaattccc gagtataaaa gcgattccac tgggcattga ctggcagagg
     1201 aatattcatt aaaaccacat cgaatcgttc gtcagttttc cctaaatata aagctggatc
     1261 agcgaaaatg acctgaacat tggtgtctaa cgcatcaatt gatccgaatt gagtaaccat
     1321 ttgatgcaga atccggtcag gttgaaggat tgtaatacgc tcgactgacg gatgccgact
     1381 aatttcgcga cagatttcaa tgcttccgat tccaatgact aaaaccgatc gcggatcagg
     1441 atgattcagc aaagcaaaat gcgtccactc ctcggctttc tcacggtctc cgaaagtcca
     1501 gagcggctga ctgtcgccat agacactgat ggcgtcctgg taacgcacgg tagtcaacgc
     1561 aagatgcggc gattcgcggc tgagcaaagc cctaaacggt gcccatctcc agttagtaat
     1621 tggccctcgg aaaccaactg aagccaaaat aactatcagc gaagccccga tgattaaaaa
     1681 tattcgcatt cgttcggagt aaataaagaa tccgatcgcc aaaaccatca tcacccatac
     1741 aattaccaac aggcaatcta tagtactcac tcccgataga atcaagccag caaaaagtaa
     1801 acttcccaaa gccgagccga ttacttcacc agcgtaaact gtatgaaccg gggaaagctc
     1861 cgctttaact ttgacccagt tcacgattag cggaaaaaac aatccattca accaggctgg
     1921 cagactgaaa aggataaaag tccaggcgaa aatcgtcccc aagccaacta ctgtgccagc
     1981 gagaatcccg gcaatccggc gatttaaaaa taacagtccg gcaaaaataa tcagaaccat
     2041 tccgtagctt agaacgattg cggtcaacca tttttctgat aaattgcgcc gcactaatcg
     2101 cgcgcctacc aaactaccca gcccagtcca gattaaccag tgaccgagtg caatgcacat
     2161 cgtcagttca ttgccgtaaa atgactgagc cgtcaggcgc aaatatgcaa cctgcgcaat
     2221 catagcaacc gcgcccatca cagcaggcat tatggcaaat tttacgtaat ctcgcctcat
     2281 aaaaatgcct atgcgaaaat cttaaggtaa attttattag taaacactat tccgaaactc
     2341 ggaccgcgtc aggatcccag gcgactctga atccgagaaa acttagccga gcaaccggca
     2401 tatagcgata acgactggtc gttcgcattg cttctttgcc atgataccag cttcctcccc
     2461 ggataacttt gaacataccg gaagctggcc ccgtgggatt tatttggctg gaatttttgt
     2521 agtaatcact gcggtaataa tcttgacacc attcataaag attgccagcc atatcataaa
     2581 gaccgaggct attcggtgga aattttgcca ccggcgcaac tcttttccat ttgtcagttt
     2641 gaccggttcc ggaataatta gcaaatgaat gatcaatagt attgccattc gggtatttca
     2701 agttcaaacc cctcgaacga gcggcatact cccattgcgc ctcagtaggc aaataacccc
     2761 caacccactg acaaaattcg ttggcttcat accaggtaac aaatgcaatc gggtaagaat
     2821 cattagttcc cgcttcctgt tccggcatag ctttacctgt ttgacgacaa tactctttgt
     2881 attgaccgac agtcacctcc gttacactaa tgtaaaaatc agtcagcgtg acggtatgag
     2941 ccggtcttgt atcctcggga ctactttcat catcagagcc catttcgaaa gtcccaccct
     3001 tgaccagcac cgctggtagc tttaaaactc caattcttac cacattctga gccacgtcac
     3061 tcgtctttgg aaccggttca ggagcatcac tgaaaagccc aacagcccaa attagaaagc
     3121 acagaaaaag aattgttaac tttcgcgttt gctttttcat ttcctattac atccctaaat
     3181 ctataattaa actataaaaa gaacttcgga atatcaagga ctttttatct cgtactcgct
     3241 tttgacgact taaatgagct ttaaaaacac gataaaattc cacatatcgt cagaattatt
     3301 tagaaatggc aattttttat gttatttaaa aacaaagttt gttaatattg gatatgttaa
     3361 tcattcctaa aaaggaatcg ttcaatgaag aactacctaa acaatttaaa aaccggattg
     3421 atcatatttg gtttgattgc cagtatgatc acctctatac cgaaaggcgc taacgaaatg
     3481 caaaccctca ctgttcatta ccaccgctac gatggagctt accagaattg gacgctctgg
     3541 acctggtctg aaaatttcag caccgagatc aaagcgaccg gtcaggatga ctatggtctg
     3601 atttttaacc tcgatctgag tatttatccc aatcccaaaa gtatcggtct tttaccaaag
     3661 tatggcagtt gggaaagaaa agatgatccg gatcgttact ggaaaccgaa ttcccatcat
     3721 gaagtctggc tcttacaggg cgatgaaaca gtctataact ctcgtcctga tataagcccc
     3781 aaaatccgcc gggcttttct cgatagtcct accaccatta cgctcgtctt ctccaatgca
     3841 attaccaaag aaatgtcaac ttccctcgcc ccaaccatca aattgaaaga tggtcaaaca
     3901 atccagcctg gccgaatttg tctgattccc gaaaactccc attcctcgga aatagtaaga
     3961 ttattggatt tgcctgcgat tgaaattgat aacttgccgg caaccgtttc aatacccaat
     4021 ttcgcggatt gtcctgtctg gctcaggtat attcgagatg atgaaaagtt cataacccag
     4081 gagccacttg gcgcttttta ttcacccgac tctaccaagt ttgcaatttt cgcgcccggt
     4141 gctacggcag tagtgctcca tatttatgat caaccgcaag gtggcaagcc taccaaatat
     4201 aaattgacca agattcagaa cagcctctgg tctgtaacgg tgaaaggtga tctgaaaggg
     4261 aaatattatg tttatagcgt cgccggtccc gatccggctt atgaaccgaa aaaagaggtt
     4321 ctcgacccta ataccacctg cgctactact cacgatggac gggctttgat tttcgaggaa
     4381 aatactcaga tagcggttgc acctaaattc ctcctaaaag atgcaattat ctatgaaatg
     4441 cacgtccgcg actttactat cgccgaaaat tccggtgtaa agaacaaagg caaatacctc
     4501 ggtttcaccg aagaaggcac aaccattccc ggcacttcaa tcagcaccgg tctggaccat
     4561 ctaactgaac tgggaatcaa taccatccag ctactaccca ttcaagattt cgaacatgac
     4621 aacaaggtta acaattactt ctggggctat atgacggtca attttaattc gccggacggt
     4681 tggtacacca ccaacgaaaa tgacgagagc cgggtgcgcg aggtcaaaca gatggtttct
     4741 gcccttcaca aacgcggcat caaagtcgtt atggatgtag tttacaatca taccgccgaa
     4801 accaatccgc aaatccgtta taatttcaat gggattgtgc cgagctttta ctatcgccaa
     4861 agagaggacg gatcttattg gaacggttcc ggatgcggca atgaagtgcg cagtgaaaat
     4921 ccgatggtac gtcgctttat catcgaatca ctcaaatact gggtcaagac ttatgacatt
     4981 gacggatttc gcttcgattt aatgggatta attggcatag aaactatgcg ccaaatcgtt
     5041 aaagaactaa ccgcccttaa gcccgacatt tttctctatg gcgaaccctg gattgtcggt
     5101 gaaacgccca tcaaaccgat tttgaaaggc gctcaacgcg aagaaggctt cgccgtattt
     5161 aatgacaact tccgtgacgc tctcaaagga ccgtggcata atactgatcc gggatacgtt
     5221 caaactggca aaaatatcga agccgttaaa atcggcattc ggggtagtat cgacgatttt
     5281 gctgctgatc cgatcgaaac cattaattat gtttcctgcc acgacggccg cacactctgg
     5341 gatcaattag ttgcctccac cggcaacaat aaaatgtata ccgacacgca actaaaagca
     5401 atggataagc tagccgcttt tatcattttc acctcgcagg gtattccttt catgcaaggc
     5461 ggtgaggaat tcctgcgcac caaattcggt tccaataata gctacaacca gccagacaaa
     5521 atcaataaga ttcgctggga ttacaaacag gaaaatcatg acattttcgc ctattatcag
     5581 ggactaatcc gtatccgcag agaccaccca atgttccgaa tggaaaccgc cgaagaagtg
     5641 cgccgcaatt tggcattttt tgaagaactc ggactcagcg taccacccaa ttgcatagcc
     5701 tatcgtctcc agcgcggtac cagcggcgac atctggcgcg aagttattat cctaatcaat
     5761 ccgaacagcc gtccggaaac ctttaaaatc ccaaaagcta aatggattct ggctgtagat
     5821 catacttatg ccgatccgga aattatttcc ccgattagtg gacctttagt taccgttaaa
     5881 cccatttcgg caatgctgat ttaccgcctt taattacagg taatcctccc taaagaacta
     5941 gatgaaaaca actcgatttc attcttgttt acgcttaatt gttttaccct tgctgatatg
     6001 cggtctcttt aactgtaccc aacggaacgt attaaataaa ccaccggaaa taatcgaatt
     6061 tgttcgaatt gattccggat gtagtacatc attgagtctc tctgaaatat ttaccgcaaa
     6121 gaaatataaa ccgattttca gtcccaaccc ggatattcaa ttagtctatc aacccgattc
     6181 caatataatt attctgactc cgaacaactc attttccgga ttgactatga ttaattttac
     6241 caaccagaaa aaagatcttt gtctgccgat cattgtcaaa caaaagaatc tggtcacctt
     6301 ttcctataag cctcccaaac cggttcggga aatattcgta atgggtaatt ttaatgattg
     6361 gaatcggagc tcattaccaa tgtctgatga agacagtgac ggaattttta cacgggatgt
     6421 ttggctcgac gatggtttat acgaatacca gttcgttgtt gaccagaacg agatttatga
     6481 tcccgccaat ccggaaaaga ccgataatgg atttggctat ttcaattcgg ttttacgcgt
     6541 tacggctccg ctggctgatt atgccgccaa tctatatatt ttgccgacca gacagaacga
     6601 tactattacg ttagctgttg actgcctcgg tgataccagc gatatccgtt atttcgtctt
     6661 gctggataat catctttgtc cgcccaaaag ctatcatcat aaaaataaac aactgcagat
     6721 ctatttaaat tcacttgaaa atacgatcgg gatgcatatt ttgcgggcaa cggctcaata
     6781 caaaaatcaa cccggcaatg tgatcactat ctatttgaaa aatggtaaac cactcgataa
     6841 taataccttc atttggaacg atgcttgtct ttacgccata atgaccgacc gttttaaaaa
     6901 cggcaactca ggaaacgacc gaccggtagt tcaccctcac ttagcgtcac aagcaaattt
     6961 caagggcggt gatttcgacg gaatccgccg gactatcgaa tccggttatt tcgacgacct
     7021 cggcatcaat actctctgga tttcaccggt caataaaacc actgatagtg cttatcagga
     7081 atggcctgag ccccataatt acttttccgg ttatcatggt tactggccaa ctgatgctag
     7141 acagactgac tcgcgttttg gctccctcca agaattaaaa aaattagtcg aggtggctca
     7201 tcgtcataat atcaaaattt tactcgattt tgtctccaac catgtccata tcgagcatcc
     7261 ctatttcaga gaacatcgtg agtggttcgg tcaattggag ttgccagacg gatctctgaa
     7321 tatccggcgc tgggacgaat atcgcctgac aacctggttc gatacttttc taccgtcttt
     7381 tgattacgaa ggctcgcacg aggcccttca aactatgact gacaacgctg tctggtggct
     7441 aaagcaaact gatgcggacg gttttcgaca cgatgctacc aaacatgtgc cccatgcttt
     7501 ctggcaaact ctgacatcca aaattaataa gcggatcaat cctcaccgga atctggatat
     7561 ttttcaaatc ggtgaatgtt tcggcagtaa tgaattaatt aaatcttatg ttaataatgg
     7621 aatgctcgaa tcgcaattta attttaatca atttttcact gctcgtcggg ttttcgtgga
     7681 accgaccggc gactttcgcg atttggagct ggctattaaa aaggcgttgg aagtttatgg
     7741 atacaatcac ctgatgggca atttaatgga tagtcacgat caggtgcgca ttatggcttt
     7801 tttggatggg gacttgactt tgtctgacaa cggcacgatg cgcgcctggc aagaaccgcc
     7861 cataacggtg gataatccgc ttacttaccg gaaagaatta gtcttcctcg gatatatatt
     7921 gactgtaccc ggaattccgg ttattgatta cggagatgaa ttcggcttga ccggcgctaa
     7981 cgatccggac aatcgtcgta tgatgcgttt tggtgaagaa ttatctgatc tggaacgaga
     8041 acaattacag caggtaagca atttaatcca attgaggaat atgcatccgg ctttacgacg
     8101 cggcgattat ttaccactga aagccgaaaa ggacattttc atttacagcc gtggcgatgc
     8161 caatgaacgg cttattatcg cattaaataa aagcccggat attcaaaatc cgataatatt
     8221 tttaccggat tggttaagag gccaatctct acgatcgtta accaataata acacctggcc
     8281 aattgattat aatacggtca aggttagttt agccggttac agtgtcgata tttttaccat
     8341 taatccgtag gacagccttt ccaggctgtc atcgct
//
LOCUS       DAOOMU010000092         8203 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_10250_length_8203_cov_13.998405, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000092 DAOOMU010000000
VERSION     DAOOMU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10250_length_8203_cov_13.998405"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /gene="araA"
                     /locus_tag="PLI55_08400"
     CDS             complement(<1..1472)
                     /gene="araA"
                     /locus_tag="PLI55_08400"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016776897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HPB00890.1"
                     /translation="MKVFENYEVWFVTGSQHLYGEDVLRQVADHSREMAKYLSDSPNI
                     PAKVIFKSVLTEPEAILHLALEANSAKNCIGLIVWMHTFSPAKMWIAGLKNLQKPFVH
                     LHTQYNRDLPWSEIDMDFMNLNQSAHGDREFGFICSRMKINRKVIVGHWRDENVINEL
                     AIWVRAAVAWQDSRTGKIARFGDNMREVAVTEGDKVEAQIRFGYSVNGYGIGDLAKVV
                     QNIPEADIDKLIEEYNAKYIVAKEAAKGGEQYDALREAARIELGLRQFLKCGNFTAFT
                     TTFEDLHGLKQLPGLAVQRLMADGYGFGAEGDWKTAALVRAMKIMATGLTGGTSFMED
                     YTYHFNPSGMKVLGAHMLEVCESVAANKPRLEVHPLSIGGKADPPRLVFNAPTGPALN
                     VSLIDLGNRFRMILNEVESVQPDADLPKLPVARVVWMPKPNLKTAAQCWILAGGAHHT
                     GFSQALTAEYLRDFAEIAGIELVEINNSTNVTEFKKELRW"
     gene            complement(1491..2186)
                     /locus_tag="PLI55_08405"
     CDS             complement(1491..2186)
                     /locus_tag="PLI55_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008722423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HPB00891.1"
                     /translation="MLEELKAEVLNANRALVQYGLVILTWGNVSGIDREKNLVVIKPS
                     GVEYDKLTIDDLVVVNLDGRVVEGNRRPSSDTPTHLALYRAFDNIGGISHTHSDYATT
                     FAQAGCAIHCYGTTHADYFNGSIPITRLLTESEVNQNYEMNTGQVIIERFQDLNPLAM
                     PAVLVAGHAPFTWGKNATEAVKNSFILEKVAQLAFQSILLNENLTELPEYILQKHYLR
                     KHGPSAYYGQNDK"
     gene            complement(2171..>2491)
                     /locus_tag="PLI55_08410"
     CDS             complement(2171..>2491)
                     /locus_tag="PLI55_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HPB00892.1"
                     /translation="TGRPMKVSTSEQTPALGAAIFAAVSAGKAHGGYDGVTEAQRAMT
                     STGKDYLPDNRNHQTYKELYDLYKQMHDAFGTPEWSGKMFNVMKDLLNIRDRVRQEKL
                     CSKN"
     assembly_gap    2492..2591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2592..3716)
                     /locus_tag="PLI55_08415"
     CDS             complement(<2592..3716)
                     /locus_tag="PLI55_08415"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HPB00893.1"
                     /translation="MVKTKKYTIGLDFGTNSCRSLIVDISNGRELATHVFNYPSGEAG
                     IIVDSANPNLARQNPADYLLGLEVTIREALRKASQIEPDFSPKDVIGIGVDTTGSSPM
                     PVDQEGMPLCFQAKFKDNPSAMVWLWKDHTSYAEAQKITELARQLRPQYLAKIGGVYS
                     SEWFWSKLWHLQSECPEVFRNTFSFVEICDWIPAILTGNTDPLLIKRSICAAGHKALF
                     NEQWGGLPDEEFLKELSPEIAAMRQRLYRKAYSAEEKMGNLSPEWAEKLGLTTEVAVA
                     VGAFDAHMGAVGAGIATGILVKILGTSTCDIMVWPNSEKLNDIPGVCGIVDGSVLKGY
                     FGIEAGQSAVGDIFLWFVNNLVPDSFGKTQEEKFENLAKAA"
     gene            complement(3750..>4270)
                     /locus_tag="PLI55_08420"
     CDS             complement(3750..>4270)
                     /locus_tag="PLI55_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/glucose cotransporter"
                     /protein_id="HPB00894.1"
                     /translation="VATAVMVLIGLIWIPVIRGGKGLYDYLQGVQSYLAPPIFVVFFL
                     GVFWKRANAKGCLATLITGFTLGLFRLAVDTPVKLLNVKYAEGSFLWIVNNIYFQYYS
                     LLITIISILVMIFVSLGTKEPDYEKIKGLTYGTRTEEDRKELRANRSRGDVIASVILV
                     VLIVLCYIYFSG"
     assembly_gap    4271..4370
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4371..5317)
                     /locus_tag="PLI55_08425"
     CDS             complement(<4371..5317)
                     /locus_tag="PLI55_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096059.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HPB00895.1"
                     /translation="MLQTLDWIIIVGYFGIILGIAWWVMRQKQETSTDYFLASRNLGW
                     FIIGASIFASNIGSEHLVGLAGSGATDGVAMAHYELHAWCLLILGWVMIPFYMRSKVF
                     TMPEFLERRFSPRARTVLSLISLVAYVLTKIAVGIFAGGIVFSVLLPDLNFMGLDSFW
                     VGSVLVVVLTGLYTILGGLKAVAYTEALQTIVLVFGSILVTLFGLKALGGWGELREIA
                     GSDMFNLWKPLIPHGVEGTWAPIKESGRMAWYFNDNYPWIGMLLCAPIIGLWYWTTDQ
                     YIVQRALAARDIKTARRGTIAAGFFKLLPVFIFIIPGIIA"
     gene            complement(5345..6472)
                     /locus_tag="PLI55_08430"
     CDS             complement(5345..6472)
                     /locus_tag="PLI55_08430"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPB00896.1"
                     /translation="MDKKLLIAITALTSILTINCAKKEQGSQASVEIFGTLSNGTEIK
                     KYTISNSKGLTAEIINYGARVVSLKAPDRTGKIEDIIFGFDDLASYERDNSYQGAIVG
                     RYGNRIRDGKFTLEGKTYQLDINDGENHLHGGKDGFYRQVWECREADSRSIQLCYISP
                     DGEGGYPGQVEITVIYTITDDNGLKIEYSAKTDKTTILNPTSHCYFNLSGFPEKSILD
                     HELMINADYFTPVDAGLIPTGEIRPVVGTPMDFRTVKAIGKDIEADDEQILYGNGYDH
                     NWVLNDYNGQVRLSATLYEKETGRLMEVLTDQPGLQFYSGNFLDGSMTGKGGVKYNHR
                     TALCLEAQAFPDSPNKPNFPSVVLRPGEEYKQTTIYRFSTK"
     gene            complement(6684..>8203)
                     /locus_tag="PLI55_08435"
     CDS             complement(6684..>8203)
                     /locus_tag="PLI55_08435"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPB00897.1"
                     /translation="LYFAAQAYYNNRDWPGNNQKYWRARSGSKTKWRWIIYDLDFGFN
                     LYESDGHKEDHISFMFTVNNSNYAQVEWAIKLQKKLIENPTIRNRFINQIADLLNTNF
                     KSERVVNTINEMADHIADELPRHRQRFGIGGENRDNMITFAKQRPAYLRNHVRNYFKC
                     GVDGNLTIDITEGGTVKLNTLSLQAADAPWTGIYFQGNAVNLEAIPKPGYKFDGWSGA
                     VTSSDVSLSINVTISTNLIATFSATNNAVKNVVINEINYNSADDFDSGDWVEFYNNSD
                     SPIDISDWFFKDNDDSHIFTFPSGTILESSAYLVLGEESEKFIACYPDVTNFVGEMNF
                     GLSGSGELIRLFDSEGQIVDSLTYSDKAPWPAEADGQGATLELFAPNLDNTNGENWRA
                     SVGHGSPGQQNTVTVAIAPNDSREIPEKFNLMQNYPNPFNHSTVILFQISEPSMVKLS
                     VYDLNGRLIETLVNEYRNPGYYAVHWNAENVVSGIYFYRIEAGSLSSVKKCLLIK"
BASE COUNT         2267 a         1894 c         1554 g         2288 t
ORIGIN      
        1 ttccaacgca gttctttttt gaattcggta acattagtgc tgttgttgat ttcaactagt
       61 tctattccgg cgatttcggc gaagtcgcgc agatattctg cggtcaaggc ttgactgaag
      121 ccggtatgat gagctccacc tgccagaatc caacattgag cggcagtttt caggttaggt
      181 ttaggcatcc agactacccg agcgactggt agttttggta aatcggcatc cggttggaca
      241 ctctcgactt cgttgagaat cattcggaaa cgattaccaa ggtcaatgag cgaaacattc
      301 agcgccggac cggtcggagc attgaatacc aaacgcggcg ggtcagcttt gccaccgatt
      361 gagagtggat gaacttctaa gcggggttta ttggcggcga cagattcaca tacttccagc
      421 atatgtgcgc ctaagacttt cattcctgat ggattgaaat gataagtata atcttccata
      481 aacgaggtgc caccggttaa gccggttgcc ataattttca tcgctcgtac taatgcggcc
      541 gttttccaat cgccttccgc tccaaaaccg taaccgtccg ccattaagcg ctggactgcc
      601 aaaccgggaa gttgcttcaa accgtgcaaa tcctcgaagg ttgtcgtaaa tgcggtaaaa
      661 ttgccgcatt ttaggaattg tcgcaaaccc agttcaattc gggcggcttc tcgcagagcg
      721 tcgtattgct cgccaccttt agcggcttcc ttggcaacga tatatttagc attgtattct
      781 tcaatcaatt tatcaatatc ggcttcagga atattctgaa ctactttggc taaatcgcct
      841 attccataac catttactga atatccgaac cggatttgag cttcgacttt gtcgccttct
      901 gtgacggcaa cctcgcgcat attatcgcca aagcgcgcga ttttcccggt gcggctatct
      961 tgccaggcga ccgcggcgcg gacccagatt gccaattcat ttatgacgtt ttcatcgcgc
     1021 cagtgcccga caatcacctt gcggttgatt ttcattcggg aacaaatgaa gccgaattcg
     1081 cgatcgccgt gggctgattg attcagattc ataaaatcca tatcaatttc cgaccagggc
     1141 agatcgcggt tatactgggt atgcagatga acaaatggtt tttgtaggtt tttcaatccg
     1201 gcgatccaca tttttgccgg tgaaaaagta tgcatccaga ctatcaaacc gatacagttt
     1261 ttggcgctat tggcttccaa cgcaagatgc aaaatcgcct ctggttcagt taaaactgat
     1321 ttaaaaatta ctttggcagg tatattcggc gaatcagata ggtacttagc catttcgcgg
     1381 gaatggtcgg ctacctggcg caaaacatct tctccgtaca ggtgctgact gccggtaaca
     1441 aaccaaactt cgtaattttc aaatactttc attttttatt cctttattga ttatttatca
     1501 ttttgaccgt aataggctga tggtccatgt ttccgtaagt aatgcttttg caggatatat
     1561 tcaggtaact ccgttagatt ttcattcagt aaaatgcttt gaaatgccag ctgggcaacc
     1621 ttttccagaa tgaagctatt tttaaccgct tcggtggcat tttttcccca ggtaaaaggt
     1681 gcatgcccag ctactagaac ggccggcatt gcgagcggat tcaaatcctg aaaacgctcg
     1741 atgataacct gcccggtgtt catttcgtaa ttctgattaa cttccgattc agttaataag
     1801 cgagtgattg gaattgaacc attgaaataa tcggcgtgcg tcgtgccata gcaatgaatt
     1861 gcgcaaccgg cttgagcaaa ggttgtagcg taatcgctat gagtgtggct gattccgcca
     1921 atgttgtcaa acgctcgata taatgccaag tgtgtcggag tatccgatga agggcggcga
     1981 ttgccttcaa ctactctccc atccaaattc actactacca gatcgtcaat cgtcaactta
     2041 tcatattcaa cgccactcgg tttgataaca accaggtttt tctcacgatc aatgccactg
     2101 acattacccc aggttaaaat caccaaaccg tattgtacca atgctcggtt ggcattcaga
     2161 acttctgcct ttaattcttc gagcacaatt tctcctgcct tacccggtcg cgaatattta
     2221 acaaatcttt cataacgttg aacattttcc ccgaccattc cggagtacca aaagcgtcat
     2281 gcatctgttt ataaaggtca tataactctt tgtatgtctg gtgattacgg ttatcaggta
     2341 aataatcctt gccggtgctg gtcatcgctc gctgagcctc agtaacacca tcatatccac
     2401 cgtgcgcttt gccggcagaa accgccgcaa aaatcgctgc gcccagcgcc ggagtttgtt
     2461 cactagtaga aactttcatc ggtcggccgg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nggcggcttt cgccagattt tcgaactttt cttcttgagt tttaccgaag
     2641 gaatcaggca ctagattatt aacaaaccac agaaaaatat cgcccaccgc tgattgcccg
     2701 gcttcgatac cgaaatagcc tttcagaact gaaccgtcca ctatcccgca tactcctggt
     2761 atgtcattca atttctcgct attcggccag accattatat cgcaagtgct ggttcccaga
     2821 attttcacca gaatcccggt tgcaattccg gctccaactg ctcccatatg ggcatcaaaa
     2881 gctccaacgg caacagccac ttcagttgtc aatcccaatt tttccgccca ttccggggat
     2941 agatttccca tcttttcttc ggccgaatag gctttccggt aaaggcgctg acgcattgcc
     3001 gcgatttctg gcgatagttc tttcagaaat tcttcatcag gcaaaccgcc ccactgctcg
     3061 ttgaacaggg ctttatgccc agcggcgcag atgcttctct taattagtaa tgggtcggta
     3121 ttgcctgtca atatggctgg aatccagtca catatttcga caaaactgaa agtgttccga
     3181 aaaacttccg gacattcgct ttgcagatgc cagagtttgc tccaaaacca ttctgacgag
     3241 tacacaccac cgatttttgc caaatattgt ggccgtaatt gacgagcaag ttcggttatt
     3301 ttttgagctt cggcataact ggtatgatct ttccagagcc agaccatagc cgaagggtta
     3361 tctttaaatt tagcctgaaa acagagcggc ataccttctt gatctacggg cattggacta
     3421 cttccggtag tatctactcc gattccaatt acgtccttag gtgaaaaatc gggttcaatt
     3481 tgggaggctt tacgaagcgc ctctcgaatt gtaacctcta agcccagcag ataatcagcc
     3541 gggttctggc gagcaaggtt aggatttgcc gaatcaacaa taatcccggc ttcacccgaa
     3601 ggataattaa aaacatgtgt cgctaattcc cggccgttag aaatatctac aatcagcgaa
     3661 cggcaagagt tagtgccaaa atctaatccg atagtatatt tttttgtctt aaccattttg
     3721 tccagtaaat aatgttaact gcttgaatat caacccgaaa aatagatata acaaagaaca
     3781 atcagaacta ctaatatgac cgaagcgatt acatcgccac gtgaccgatt agcacgtaat
     3841 tccttgcgat cctcttcggt tctggtacca taggtcaaac ctttgatctt ttcataatca
     3901 ggttcttttg tgcccaaact aacaaaaatc ataacgagaa ttgatattat cgttatcaaa
     3961 aggctgtagt attggaagta gatgttattc acaatccaca ggaaagaacc ttcggcatat
     4021 ttaacattta acaattttac cggtgtatca actgccaatc ggaaaagacc aagggtaaag
     4081 ccggttatca aggtagccag acagcccttt gcattcgccc gtttccaaaa aactcctaag
     4141 aaaaagacca caaaaatagg cggtgctaaa taagattgaa caccttgtaa ataatcataa
     4201 agtccctttc caccccgaat taccggaatc catatcagac caatcaatac cataacggca
     4261 gtagctaccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcaattatac
     4381 caggtattat gaaaataaaa actggtaata atttaaaaaa tccagcggca atggtgccac
     4441 gccgcgctgt ttttatatcc cgagccgcta atgctctctg cacaatatac tggtcggtcg
     4501 tccaatacca tagtccaata attggagcgc ataacagcat tcctatccag ggataattat
     4561 cgttgaaata ccaagccatc cggccgcttt ctttaattgg tgcccaggta ccctcgacac
     4621 catggggaat aagtggcttc cagaggttaa acatatccga accggcgatt tcccgtaatt
     4681 ctccccaacc gccgagagcc tttagaccga acagggttac caaaattgaa ccgaaaacca
     4741 aaacgatcgt ctgtaaggct tcggtataag caacggcttt caaaccgccc aatatcgtat
     4801 ataatccggt caacaccact accagtaccg aaccaaccca gaaactatct aatcccataa
     4861 aattcaaatc cggcaatagc acactaaata caataccacc ggcaaaaatt cctacggcaa
     4921 ttttagtcag cacataagcc actaacgaaa ttagagacaa aactgtccga gctctgggcg
     4981 agaaacgtct ttccaaaaat tccggcatcg tgaatacttt cgaacgcata tagaaaggta
     5041 tcatcaccca cccaaggatt aataagcacc aggcatgtaa ttcgtaatgc gccatagcaa
     5101 cgccgtcggt cgctcccgaa ccggctagtc cgactaaatg ctccgaaccg atattcgaag
     5161 cgaaaattga agctccgatg atgaaccagc ccaaattgcg gctggcaaga aagtaatctg
     5221 tggatgtttc ttgcttctgc ctcattaccc accaagctat tccgagaatg attccaaaat
     5281 agcctacaat gattatccag tctaaagtct gtaacatagc tatttcccct tgttaaaagt
     5341 tgaactattt tgtcgaaaaa cgatagattg tcgtctgctt atattcttca ccaggtctta
     5401 aaaccaccga cggaaaattc ggcttattgg gcgaatccgg aaaagcctga gcttccagac
     5461 aaagggcggt acggtgatta tactttaccc ctcccttgcc agtcattgaa ccatctaaga
     5521 aattgccaga ataaaattgc aaacccggtt ggtcggttaa aacttccatc aatcggccag
     5581 tttctttttc gtacaaagtc gccgaaagtc gaacctgacc gttatagtca ttcagaaccc
     5641 agttatgatc gtagccgttg ccatataaaa tttgttcatc atcggcttca atgtctttgc
     5701 cgatcgcttt taccgttcga aaatccatcg gtgtacctac aacaggccga atctcaccgg
     5761 ttggaattaa accggcatcc accggcgtaa aatagtcggc atttatcatt agttcgtgat
     5821 ctaagatact tttttccgga aatccactca aattgaaata gcaatgactg gttggattca
     5881 aaatcgtcgt cttgtcggtt ttggcactat attcgatctt gagtccatta tcatccgtaa
     5941 ttgtataaat aacagttatt tcaacttgtc ccggatatcc gccttcccca tccggactga
     6001 tgtaacatag ctgaattgat ctggaatcag cctcgcgaca ttcccaaacc tgtctataaa
     6061 atccgtcttt tccaccgtgt agatgattct caccatcatt aatatcaagc tgatacgtct
     6121 ttccctcaag agtaaactta ccatcccgaa tgcggttacc gtagcgccca acaatagcgc
     6181 cttggtaaga attatcgcgc tcataagaag ccaaatcatc aaaaccaaag ataatgtctt
     6241 caattttgcc ggtacgatca ggtgctttca atgatactac tctggctccg taattgataa
     6301 tttcggctgt caatccctta ctattggaaa tcgtatattt ttttatttcg gtgccatttg
     6361 acaatgtccc gaagatttct accgaagctt ggctaccctg ctcctttttc gcacaattaa
     6421 tcgtcaaaat gctggttaga gcggtaatgg caatcaataa ctttttgtcc atctttatta
     6481 ctctccttat ctttctcaat tcaaatccta ttttgagtcc aattataata aagaaattga
     6541 ttaaaaaaaa gacgttttat ataaagccac taagtcacta aggcacgaag aatttattaa
     6601 cttttaaact acttaagcat tataaagaca atcagattaa aaaattttaa acaatgcgaa
     6661 agccttccga agatcaaacg attctattta atcaataaac atttctttac actgcttaga
     6721 ctaccagcct caattcggta aaaatatatt cctgagacga cgttttcagc attccaatga
     6781 actgcataat aaccgggatt tcggtattca ttcaccaatg tttcgattaa tcttccgttg
     6841 agatcgtaaa ctgataattt gaccatcgac ggctctgata tttgaaataa tatgacggtg
     6901 gagtggttga acggattggg gtaattttgc atcaaattga atttttcagg gatttcccga
     6961 ctatcatttg gagcaatcgc aactgttaca gtattttgtt gaccaggaga accgtgtccg
     7021 acggaagccc gccaattttc accattagta ttatccaaat tcggagcgaa tagctctaaa
     7081 gttgctccct gtccatcagc ttcggctggc cagggagctt tatcgctata ggtgagtgaa
     7141 tcaacaatct gaccttctga atcaaacaac cggattagtt caccgcttcc gcttaatccg
     7201 aaattcattt caccgacgaa attggtcaca tccggataac aagcaataaa cttttcgctt
     7261 tcttcgccta aaacaagata ggcagacgat tcgagaattg ttccgctcgg aaaagtaaat
     7321 atgtggctgt cgtcgttatc tttgaaaaac caatcgctta tgtcaatcgg agaatcacta
     7381 ttattataaa actcgaccca atcacccgaa tcaaaatcat cggcagaatt ataattaatt
     7441 tcattgatga ctacattttt tacagcatta ttagtagctg aaaaagttgc aattaggttc
     7501 gtggaaatag tgacattgat gcttaatgat acatcggaac tggtaactgc tccactccag
     7561 ccatcgaatt tatatccggg ttttggtata gcttcaaggt tcaccgcgtt tccttgaaaa
     7621 tagattccgg tccatggagc atctgcggct tggagtgata aagtgttgag ttttacggtg
     7681 ccgccttcag ttatatcaat tgtcagattc ccatccacac cacatttgaa ataattccgc
     7741 acatgattgc gtagataggc cggtctctgt ttggcaaaag ttatcatatt atcccgattt
     7801 tctccaccaa ttccaaatcg ttgtcggtgt ctgggaagtt catcggcaat atgatctgcc
     7861 atctcattga tagtattgac aacccgttcg cttttaaaat tggtattgag caaatcggcg
     7921 atttgattga tgaatcggtt tcggatcgtc ggattctcaa ttaatttctt ttgtaatttt
     7981 attgcccatt cgacttgagc ataattgcta ttattaaccg taaacataaa agatatatga
     8041 tcttccttat gaccgtccga ttcataaaga ttaaaaccga aatccaaatc gtaaataatc
     8101 cagcgccatt tagttttaga acccgacctg gcgcgccagt atttctgatt attgcctggc
     8161 caatcgcgat tattataata ggcttgagcg gcaaaatata aaa
//
LOCUS       DAOOMU010000093         8186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_10277_length_8186_cov_17.490592, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000093 DAOOMU010000000
VERSION     DAOOMU010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10277_length_8186_cov_17.490592"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="PLI55_08440"
     CDS             <1..1395
                     /locus_tag="PLI55_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00898.1"
                     /translation="TVGWLIPLWAQTRLTGGKRPSWIDHPAPGYYIGISRRWAEEADS
                     RADALNNAKRQIIESLGGVIESEFIDRIIETDGTVESVDAFTDSRIKVVAKNIINVKP
                     EKVYTEQWQEGKGRQKKILYQTYMAVYFDENAHRAFMKQLVEETVQLGEIQLSSAITL
                     ARQGRIFLAIDQIKAATQHLQPLTEITGIAPTDLIKIKTLNGQMLDWINLLQNGIRIE
                     GLGDKQTTKWGAGLPEPLQVSVFWQESGKRYPISGLPVEFKLLAGKAAFNPYSQTDAS
                     GCAFCSVREIRTAGRVEFEAQVTFPEGYQMAQTSTRFNLLPDNKVIVMVSETNFYHPV
                     NTPILGNLLLQKLTESGFHILEANPFSRFTGSQIEQMSPTEIQQAATNSGADLILLAS
                     VASDQPNRVQDGFYFAHARGVLKVYNISQQAFVGNYLVEDKNAGNSPENAGAKAIKKV
                     SDALIQKFSDEFGL"
     gene            1569..5990
                     /locus_tag="PLI55_08445"
     CDS             1569..5990
                     /locus_tag="PLI55_08445"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPB00899.1"
                     /translation="MLISLNKKIILIGTILAFLPLSVLATNYYVRKSGNDSNNGLTPA
                     TAWKTVTKATNYNLQPGDTVFIGTGIYNEQLIPSRSGSSTAYIVYYGDYKGLKTQDPA
                     GRVTIGGLAAGSPVFVPQSASQNNFTFTLANIINNGDGTSTIKVGIRNNNRSNLNNTA
                     ISLPSGVTALYPANGSTWTSPKNIKYTINNPYSNPFYCIRYNRSSGNVYRGGSDTLIY
                     KLTTQQAEALTSVRIRATTVSGTTGDVTLSVVYQSPSGTSYDIACKVNDKDNLVFQGI
                     TFGYVSNKAIDLYNTTNIVIRDCDTRNATNHGIYASSWDLHGVLTIESDSILSGNSAG
                     ISVQASTAHTDFVININNNYISDCGKGIELNRAYLKNGNINNNIIRNSSSHAIYIYQP
                     TEKALSVSHNQISNVTSGSGLYLYQCGVQNLDDNKISNTGSHGMYISDATSQYVPKIN
                     TICNNEVSNVNNNCHGIYLDRVYVGQIANNLIFNITSHGIYADQVSQFAILALDSNLV
                     YNCTGSGIYTMQTMSFNSVKGNTIYLVQNGLYLYANNFFSVANFSDNRIFSYSLQGIY
                     CKYLQNTTIENNLVYGCTGSGSSTYGIQIYNDNLYSNTIKNNTIYNGGYYGIYGNRTT
                     GSWRNNIVTGSTYGFRDNVSSGITATYNCAYNNTYNYSNITPGTGSITQNPLFVDPDG
                     ADNILGGNNWLDDDLHIKSTGGSYHFGAWSFDDQDSPCLDTGDPNDDYSREPEDNGDR
                     INIGCYGNTRQASKKKSACPMTAVYKNFPNNKWVMIGVPIAPTEGTPPGDPLAIFGDD
                     FGGKMPDGTNWTCIHWVTEDSVMEYYEYGDGTIYQPPTPYPGLGYFVWQNTGAPVNVD
                     VRGCPVDHCLLEVAQAPYVDWESEYGVALGFNQFANPYYFTIDWSNTEIFKYASADKT
                     GTPVKYTLSEAAANGWISQYAYTWDHNLSQYNIVVPNANNHGDSISVWQGFYFTQIDS
                     VSYLRLNIPNRRVLEKPSPISGNLQSFGEKYTYRSSSVSPEWDWFLKIGVVSKELKLQ
                     DTENGIGTAAAAKDGFDGLDALEMLGTDVAGNYLQFKFVIDRAPEIAYDLHAPFETSS
                     TWKIQVETPAANKQKTAEIVWSQIRLVPQNLKFSLLANDNSTVLVEDLRTATSYKFKI
                     KDELTTFYLKVDKVNDSVAPTFSFLFISNPLAPNDLTFFVIPSEPLESITATVNEASV
                     NLTPVASPPNIYYGKYYISSSGSVNIAVSGWDASGNSGSGSATMQALLAKSDATEKIT
                     LSDGGIVIELPAGSLSEKTAVYAARCAMDLSLLDENTPIGEPLYIGPDNVKFNQKAVL
                     HMSDLAESENVGLYRLTDGTWEFVGQYQNSGVPINSTGTYGFFSHKADQSQPDNTPLV
                     FAINNCYPNPFNNTTTIRYTVETYGKVRLTVYNLLGQTVRNLVRENQYAGAYLVTWDG
                     RNNNGKQVASGIYYLKMEVLNGDKVLFQSSKKLMLVK"
     gene            6000..6923
                     /locus_tag="PLI55_08450"
     CDS             6000..6923
                     /locus_tag="PLI55_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPB00900.1"
                     /translation="MRKRVILNFFVWGLITAGFLSRCEKIIYTYDDQKPQITTPLTIS
                     VTDSSAEIYWETDEACHIKIKYGLTTDYGLEYEDPENCEIHLATLRGLEPHALYHLQV
                     YCWDFAANGPVKSPDTTFTTLANEYSYLREGWQFFREKNYSAAKTAFDSAHQINPFLA
                     EIQAAYGWLQIRTNDLDSARQNLTAAYNLDHYLPITLAGLAVISMIDDAPLAAINYVK
                     AVLSLDQQWEYRYNPEISWKSLLLIRAQAYFQTNQMNLAQADLDSLWPQNGLNPDLPS
                     SWVVNKQSYDTYESAFGAALNYLIANPEKMG"
     gene            complement(6939..7163)
                     /locus_tag="PLI55_08455"
     CDS             complement(6939..7163)
                     /locus_tag="PLI55_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HPB00901.1"
                     /translation="MASEKETEDKNISRRNFLKKLAATAVFTVPTIQSFSLLQTQPDG
                     GWWWWTGHHHHHAPGPGEPTPPLPPPPPGL"
     gene            7269..>8186
                     /locus_tag="PLI55_08460"
     CDS             7269..>8186
                     /locus_tag="PLI55_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00902.1"
                     /translation="MTITQKIILTISLAFVTLSLINCEKEVIKVIDDLDPPVILTEPD
                     VEIAANRAMIFWTTDEPCSVSVKYFVIGNADTLTASGREYRQNHQVLLSNLLPNTIYR
                     FFTLSYDVFGNFTKTALDSFTTALDTTYFYSHGWDLYAAGDYTGACESFQQYLTYYPN
                     DLAGLTALGWSQLQDSLIDSSIVSFNSALNLDINYPDALAGLTVALYHNFHLYAMEIP
                     AVRLLATDSLYVFSHDERYDHRVIRLMLADFYNMDGRLEKAQQIIDLLYPANGLDPND
                     PNTWKIDDGEEILTFDTYESALTSLIEYLK"
BASE COUNT         2281 a         1868 c         1811 g         2226 t
ORIGIN      
        1 acggtaggtt ggctaattcc gctttgggct caaacgcgat taaccggggg aaagcgtccc
       61 agctggattg accacccggc gcctgggtat tacatcggca tttcgcgtcg ttgggcagaa
      121 gaagctgaca gccgcgccga tgctttgaac aacgctaaac gtcaaataat cgaatccctc
      181 ggcggcgtca ttgaaagtga atttattgat cgcatcatcg aaactgacgg tacagtggaa
      241 tcagtggacg cttttaccga ttcacgcatc aaagtcgtag ccaaaaatat tataaacgtt
      301 aagccggaga aggtctatac ggaacagtgg caggagggca aaggtcgtca aaagaaaatt
      361 ctatatcaga cctacatggc ggtttacttc gacgaaaatg ctcaccgcgc ttttatgaag
      421 caattagtgg aggaaaccgt tcagctgggc gaaatccaac tgagttcagc aatcacctta
      481 gcccggcaag gtcgtatttt cctggcaatt gatcagataa aggcggctac tcaacattta
      541 caaccgctga ccgagattac cgggatagcg ccgactgatc tgataaaaat aaaaacctta
      601 aacggacaaa tgttggactg gattaatctc ttgcaaaacg ggatccgcat tgaaggtctg
      661 ggtgataaac aaactaccaa atggggcgcc ggattacctg aaccgttgca ggtgagcgta
      721 ttttggcagg aaagtggcaa gcgctacccg atttccggac tgccagtcga gtttaaattg
      781 ctggcgggca aagccgcatt taatccctac agtcaaaccg atgccagcgg ctgtgccttt
      841 tgttcggtca gagaaatccg taccgctggc agagtcgaat tcgaggctca ggttactttt
      901 cccgaaggct atcagatggc acaaaccagt acccgcttta accttttacc ggacaacaag
      961 gtaatcgtta tggtctctga aaccaatttt taccacccgg ttaatactcc gattctgggt
     1021 aatctgcttc tccagaaatt gaccgaatcc ggctttcata ttcttgaggc taatcctttt
     1081 agtcggttca caggctcgca gattgaacaa atgagcccaa ccgaaattca gcaagccgcc
     1141 accaatagcg gtgctgattt gattctgctg gcgagcgttg cttcggacca acctaatcgg
     1201 gttcaggacg gattttactt cgcgcacgcc cgcggcgttt tgaaagttta taacatttcc
     1261 caacaggcgt ttgtcggcaa ttatctggtt gaggataaaa acgccgggaa ttcaccagaa
     1321 aacgccggag ccaaagctat caaaaaggtt agcgatgctc ttatacaaaa attcagcgat
     1381 gagtttgggc tttgattgaa atttgttttg tggtataatt caggtggctt tttattgaaa
     1441 tggttagaaa gaactcataa tggactgtag agtaggtatt ttaagcattt gatttttatg
     1501 acaatggtca caaacactac ttcaataatt tatgttctta aaaagatttt aattggcgga
     1561 ggaaattcat gttgatttcc cttaataaaa aaattatcct tattggaacg atactggcat
     1621 ttctaccgct ttcggttttg gctacgaact attatgtccg gaaaagcggc aatgacagca
     1681 ataacggttt gactccggca accgcctgga aaacagtcac taaagccact aattataatt
     1741 tgcaacccgg cgatacggtc ttcatcggaa cgggaattta caacgagcag ttaataccct
     1801 cgcgttccgg ctcgtcaact gcttatattg tttattatgg tgattacaaa ggattaaaaa
     1861 ctcaggatcc ggctgggcgg gtaaccatcg gaggtcttgc cgccggtagc ccggtattcg
     1921 tgccccagtc tgcttctcag aataatttca ccttcaccct ggcaaacatc atcaataacg
     1981 gcgatggaac cagcacaatc aaggtcggaa tccgcaataa caatagatcc aatctaaaca
     2041 atacagcgat cagtttacct tcaggagtca ccgcgctata cccagccaac ggctcgacct
     2101 ggaccagtcc caaaaatatc aagtacacca ttaataatcc atacagtaat ccattctact
     2161 gcattagata caatcgttcg agtggaaatg tttatagagg tggctctgat acattaatct
     2221 ataaacttac tactcagcaa gccgaagcgc tgacctcagt taggattaga gctacaacgg
     2281 ttagcgggac gactggagac gttactctta gtgtagttta ccagtctcca tcgggaactt
     2341 cctatgatat tgcctgtaag gtaaacgaca aagataactt ggtatttcag ggtattacct
     2401 tcggttatgt cagcaataaa gccatagacc tctataacac cactaatata gtaatcaggg
     2461 actgtgatac gagaaatgcc accaatcatg gtatttatgc ctccagctgg gatctgcatg
     2521 gagtcctgac tattgaatct gattctattc taagtggtaa tagcgctgga atctcagtac
     2581 aagcaagcac tgcgcatacc gactttgtca ttaatatcaa taataactac atctccgatt
     2641 gcggtaaggg cattgaactg aaccgtgcgt atctcaaaaa tggtaatatc aataataata
     2701 tcattcgcaa tagttccagc catgctattt atatttacca acccacagaa aaggcgcttt
     2761 cggtttcgca caatcagatt agtaatgtaa ccagcggtag tggactttat ctctatcaat
     2821 gcggcgtcca aaatctcgat gataacaaaa ttagcaatac cggttcgcac gggatgtata
     2881 tttccgatgc cacttcccag tatgtcccta aaataaatac catctgtaat aatgaggtta
     2941 gcaatgtaaa caacaattgt cacgggattt accttgatcg ggtatatgtt gggcagatag
     3001 ctaataatct catatttaac ataaccagcc atggcattta tgctgatcag gtaagccagt
     3061 ttgcgatttt ggcacttgat tccaacttgg tttacaattg cacgggttca ggtatttata
     3121 ccatgcaaac gatgtcattt aattcggtca agggcaatac tatctatctg gttcagaatg
     3181 ggctttatct ttatgccaat aattttttta gtgtggctaa tttcagtgat aatcgcattt
     3241 tttcttattc gttacagggt atctattgta aataccttca gaacactact atagaaaata
     3301 atcttgttta tgggtgcacc ggtagcggtt caagtactta cggtattcag atatacaacg
     3361 acaatttata ttctaatacc attaaaaata acactattta taacggtggc tattatggga
     3421 tttacggtaa tcgcacaacc ggcagttggc gcaacaatat cgttaccggc tcaacttatg
     3481 gctttagaga taacgttagt tcaggtatta ccgccactta taattgcgct tataataaca
     3541 cttacaatta ttccaatatc acaccgggaa ctggttcgat tacacagaac cctttgtttg
     3601 ttgatccgga tggagccgat aatattttag gcggaaacaa ttggctggat gatgacctgc
     3661 atatcaaaag cacgggcggt tcttatcatt tcggggcatg gtcatttgac gatcaggatt
     3721 caccctgtct ggatactggt gatcctaacg atgattatag tcgagagccg gaggataatg
     3781 gcgaccgcat taatatcggt tgttatggca atactcgcca agcgtccaag aaaaaaagtg
     3841 cctgcccgat gacggcggtt tataaaaact ttcctaacaa taaatgggtt atgattggag
     3901 ttccgattgc gcctacagaa ggtactccac ctggcgaccc cctcgccatt ttcggcgatg
     3961 atttcggtgg caaaatgccg gatggcacca attggacttg cattcactgg gtaacggaag
     4021 actccgttat ggagtattat gaatatggtg atggcacgat ttatcaaccg cctactcctt
     4081 atcccgggct cggctatttc gtctggcaga ataccggcgc tccggttaat gtggacgtca
     4141 gaggttgccc ggttgatcat tgtctcttag aagttgctca ggcgccttat gttgattggg
     4201 aatctgaata tggagttgcg ctcggtttta atcaatttgc taacccctat tatttcacga
     4261 ttgattggtc caataccgag attttcaaat atgcctccgc tgataaaacc ggcaccccgg
     4321 taaaatacac tctctctgaa gccgcagcta acggctggat cagtcagtac gcctatacct
     4381 gggatcacaa cctctcccaa tacaatatcg tagtgccgaa tgccaataat cacggtgata
     4441 gcatctcggt ctggcaaggc ttttatttta cacagattga ttcggtcagc tatctgcgac
     4501 tcaatattcc caatcgtcgc gttctggaaa aaccatctcc gatttccggc aatctgcaat
     4561 ccttcggtga aaagtacacc tatcggagtt cgagcgtctc gcccgaatgg gactggtttc
     4621 tcaaaatcgg tgtcgtttcc aaagaattga aattacagga tacggaaaat ggtatcggca
     4681 ctgccgccgc ggctaaagat ggtttcgatg gtctggatgc tctcgaaatg ctcgggaccg
     4741 acgttgctgg caattatctt caatttaaat tcgtcataga ccgtgccccg gaaatcgcct
     4801 atgacttgca tgctccgttc gagacatcca gcacctggaa aattcaggtg gaaactccag
     4861 ccgctaacaa gcagaaaacg gctgagatcg tctggtcgca gattcgcctg gtgccccaaa
     4921 acctgaaatt ttcactacta gctaacgaca acagcaccgt tttggtcgag gatttaagaa
     4981 cagcaacttc ctataaattt aaaatcaagg acgaactcac taccttttat ctcaaggttg
     5041 acaaagtaaa tgacagcgtt gccccgacct tttcctttct ttttatcagc aatccgctgg
     5101 cgccaaatga tttaaccttt ttcgtaatac cttccgaacc gctggaatcg atcacggcta
     5161 ccgttaatga agcgtcggta aatttgactc ccgtggcttc gccgcccaat atttactacg
     5221 gcaaatacta tatctcctcc agcggctcag taaacattgc cgttagcggc tgggatgctt
     5281 ccggaaattc ggggagcggc tcggcaacga tgcaagccct gcttgccaaa tcggacgcta
     5341 ctgaaaaaat taccctatcg gatggcggta tcgtgataga attgcccgcc ggttcattat
     5401 ccgaaaaaac cgcggtctat gcggcgcgct gtgcgatgga cttaagccta ttagacgaaa
     5461 atactcctat cggtgaaccg ctttacattg gcccagacaa tgttaaattc aaccaaaaag
     5521 ccgttctaca catgagcgac ctcgctgagt ccgaaaacgt gggactttac cgactgacgg
     5581 acggcacctg ggaattcgtg ggacaatatc agaattccgg cgtgccgatc aattctaccg
     5641 gcacttacgg ctttttctcg cataaggctg accaatccca gccggataat actccgctgg
     5701 ttttcgccat taacaattgc tatcccaatc cctttaataa tacgactacg attcgttaca
     5761 ccgtcgagac ttacggcaag gtgcgcctga ctgtttacaa tctgctgggt caaaccgttc
     5821 ggaatctggt acgggaaaat caatatgcgg gagcttattt agtcacctgg gacggtcgta
     5881 ataataacgg caaacaggtt gcttccggca tttattattt gaaaatggaa gtcctcaacg
     5941 gcgataaggt cttgttccaa agcagtaaaa aattgatgtt ggttaagtaa ggtagggaaa
     6001 tgaggaaacg cgtcatccta aattttttcg tttggggctt aatcactgcc ggatttttat
     6061 cccgatgcga gaaaattatc tatacctacg atgaccagaa accgcaaatc accacgccgc
     6121 tgaccatttc ggtaactgac agttccgctg agatttactg ggaaaccgat gaagcctgcc
     6181 atattaaaat aaaatatggc ttgacgaccg attatggtct cgaatacgaa gacccggaaa
     6241 attgcgaaat tcacctagct actctaaggg gcttggaacc gcatgctctc tatcatttgc
     6301 aggtttattg ctgggatttc gccgccaatg gcccagtaaa atcgcccgat accactttta
     6361 ctactctggc taatgagtac agctacctgc gagaaggctg gcagtttttc agagagaaaa
     6421 attactccgc cgctaagact gcttttgatt ccgcccacca gataaatcca tttttagctg
     6481 aaattcaagc ggcttatgga tggctacaaa tcagaacgaa tgatttggat tccgcccgcc
     6541 agaatttgac cgccgcctat aatctcgacc actatttgcc gattactctg gcgggattgg
     6601 cggtaatcag tatgattgac gatgcgccgc ttgccgcgat taattatgta aaagcggtgt
     6661 taagccttga tcagcagtgg gaatatcgtt ataacccgga aatcagctgg aaaagcctgc
     6721 tcttgattcg ggcgcaggct tactttcaga cgaatcaaat gaacctcgct caagctgatc
     6781 tcgattccct ctggccgcaa aacggattga atcccgactt gccgtccagc tgggtggtca
     6841 ataaacaatc ttatgatact tatgaaagcg cctttggagc ggcattaaat tacctgatag
     6901 ctaatccgga gaaaatggga taatgttcgg tgaaaaattt acaaacccgg gggcggtggg
     6961 ggcaaaggcg gagtcggttc accaggaccg ggggcatggt gatgatgatg accggtccac
     7021 caccaccagc ctccatccgg ttgagtctgt aaaagcgaga aagattgaat ggtcggaacc
     7081 gtaaaaaccg ccgtagccgc cagttttttc aagaaattgc gccgactgat atttttatct
     7141 tcagtttctt tttcgcttgc catattaagt accttgttaa tattgaaaaa actaatcata
     7201 atataaagtg attaattgct ataatcaata gagaataatt cgatcggtag aattcacgga
     7261 gtcttgtgat gaccattact caaaaaataa tcttaaccat ctcactggcg tttgtcaccc
     7321 ttagtctgat taattgcgaa aaagaagtca tcaaagtcat agacgatctg gaccctccgg
     7381 taattctgac cgaacccgat gtggaaatcg cggctaatcg cgcgatgatc ttttggacga
     7441 ccgacgaacc gtgttcagtt tcggttaaat attttgtaat tggaaatgct gatactctga
     7501 cagcctccgg tcgggaatac cggcagaatc atcaagttct cttgagtaat ttgttaccga
     7561 atacaatcta ccgatttttt actctgagct atgatgtttt cggcaatttc accaaaaccg
     7621 cattggatag ttttaccaca gcgctagaca cgacctattt ttactctcac ggctgggatt
     7681 tgtatgcggc tggtgactat accggcgcct gcgagtcttt ccagcaatat ttgacctatt
     7741 acccgaatga cctcgccgga ttgacggcgc tgggctggag tcaactgcaa gacagcttga
     7801 ttgatagcag tattgtctct tttaattcgg ctctgaatct ggacattaac taccccgacg
     7861 cgctggcagg actgaccgtt gctctctacc ataactttca cctctatgcg atggagattc
     7921 cagctgtgag gctactggca accgattcgc tctatgtttt tagtcatgac gaacgctatg
     7981 atcatcgcgt gattcgtttg atgctagccg atttctataa tatggatggg cgtttagaaa
     8041 aggctcagca aatcattgat ctattatatc cggctaatgg attagacccg aatgatccaa
     8101 atacctggaa aatcgacgat ggcgaggaga ttcttacttt tgatacctat gaatcggctt
     8161 tgaccagcct gatcgaatat ttgaaa
//
LOCUS       DAOOMU010000094         8156 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_10331_length_8156_cov_12.773114, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000094 DAOOMU010000000
VERSION     DAOOMU010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10331_length_8156_cov_12.773114"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLI55_08465"
     CDS             <1..901
                     /locus_tag="PLI55_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HPB00903.1"
                     /translation="ANDDSHIFWYEIAPNGKNNTVEIVCTPLNIANDIFEKVYKNVSS
                     AMLTSATLQVAGAFDYVLSRTGLNLIEDHNVVSRALGSPFYYNDQMAFFTYHQNDNEI
                     SEAFIAARLIKRLSSETGKGILVLFTSYNFMYEVYQLCLPTFIEMGVTLLAQGQGASR
                     TALLEQFRLEQNSVLLGTDSFWEGVDVVGPALEILVITKLPFPVPTEPIIEANVEKLK
                     AEGQDPFNEYYVPESVLKFRQGVGRLIRSINDVGVVINLDSRIDKKAYGRYFKKSLPV
                     EPISIVGEDELLAAVRKFFRIGK"
     gene            903..3056
                     /locus_tag="PLI55_08470"
     CDS             903..3056
                     /locus_tag="PLI55_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPB00904.1"
                     /translation="MRIFRICLCFLLSTSVILAENSKKLTVREIIDDPGLDARTYRQK
                     QFTWTPKGDGVLFLEKNKNQNCDLLKFSINSLKIDTVFTYEQLRWSNDTHSIQLDPFG
                     FISAPDGFRYLLTGENDLFLGDERDGTIVRLTNDGTQKSVPKFSPNGKWVSYICDHNI
                     WIVNCESRSSLQLTTDGNESIFNGESDWVYTEEFDFEQAYQWSPDSKMIAYCQLNENG
                     VSRYPLVDWSPMHPEIEWQYYPNAGEKIPEVKVFTVDIETQKIVCMNNFQSKNEYIVR
                     IDWLADTSLVVIQSINRLQNNQKVTYGNPRTGQTKVILEEKDPYWLNITDLYYFMKNS
                     RNFIYYSEIDNFMHLYLYDFEGNLIKPITSGDWMVTELNGVNEKDSLVYFTATKNSVL
                     ERHIYSVHIGSGAISQIDTGDGVHSAVFSPDMKYYLDFFSNVNSQVDVRIIQNNGRKI
                     TDLYHNNDFSPQKYNFGVTRFIKIPAIDGDSLYASLLYPSDFDPQKKYPVIVYVYGGP
                     GVQVVQNRYGGAWTQLLAQQGYLIFSLDNRGSYGRGRYWERRIYQQLGKIELQDQLDG
                     VKYLKSLPYIDPGRIGIWGASYGGYMTLYALTKTLEVFKTGIAISPVTDWKYYDAPYT
                     ERYLGLPINSQNIYKESAPLNYVNQIKANFLLIHGTYDDNVHLQQSLEFIDELISNNV
                     RFELMLYPGRKHGITDREGQIHMYETFLEFLQRNL"
     gene            3072..4013
                     /gene="menA"
                     /locus_tag="PLI55_08475"
     CDS             3072..4013
                     /gene="menA"
                     /locus_tag="PLI55_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HPB00905.1"
                     /translation="MSANRKISDSLKLWLRAYRAPFLTASVAPVILGTSVGWYENGRI
                     NFVSGLLALLGTIFAHFGANIFNDYYDHLTGCDDNNLDFTPFSGGSRTIQNGLLTARQ
                     AFWAGLVAFLLVVLIGLLLIVYTGRPALIIFGIVGLAIGFLYTAIPVKLAYRGLGEMG
                     IFIAFGPLIVIGASYVQTGTISFLAFLASVPAGLLVMLILYANEILDEKADRSADKNT
                     IIVKIANRNTALKIYRLILYVVPIWIIVFVIVGYFPYWSLIILCLMPKYIGTIFLSKK
                     PFNDVKEMVPLSANTIQLQFLMTITLAISFILAGITG"
     gene            4070..4140
                     /locus_tag="PLI55_08480"
     tRNA            4070..4140
                     /locus_tag="PLI55_08480"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4102..4104,aa:Gln,seq:ctg)
     gene            4171..4243
                     /locus_tag="PLI55_08485"
     tRNA            4171..4243
                     /locus_tag="PLI55_08485"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4205..4207,aa:Glu,seq:ctc)
     assembly_gap    4265..4364
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4453..4647
                     /locus_tag="PLI55_08490"
     CDS             4453..4647
                     /locus_tag="PLI55_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00906.1"
                     /translation="MCQKWACCVFYYGGILSMLLGILEKLFGFMIFDLKPSRFLMFAA
                     VCALYAIVSIMCQPVCGKKE"
     gene            complement(5193..5810)
                     /locus_tag="PLI55_08495"
     CDS             complement(5193..5810)
                     /locus_tag="PLI55_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HPB00907.1"
                     /translation="MNNEAIKISVIVVIAAYISAQMLSDITSLKIISLLGFSIDAGTL
                     IYPFTFTLRDLVHKTIGLKGTRIVIICAAVINLLMALLFWIVSYIPGDPNVGPQLEFA
                     KVLSPVWRIVIASIIAEVIAELLDTEIYRIWVNKVTKRHQWARVLVSNSLSIPIDSLI
                     FVWGAFGGIYNPSVVWSIFFANLILKGAITIISLPMIYLVKDKNY"
     gene            6043..7269
                     /gene="manA"
                     /locus_tag="PLI55_08500"
     CDS             6043..7269
                     /gene="manA"
                     /locus_tag="PLI55_08500"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HPB00908.1"
                     /translation="MENNFEIKPFVLRNKIQHYEWGTRGEQAFIPRLLGIQSEGHRPY
                     AELWIGAHPSAPSQVNISNDLIDLTELIKKHPVEILGETVFKRFGANLPFLFKVLSAG
                     KPLSIQAHPNKSQAVRLHQRDPIHYPDSNHKLEIAIAIDNLKALVGLLNIVELRKKLE
                     LYPPIQSFTDRVLPGDSTISEADETTIVKQFYSTLIRKSVGEPQLYGNLVESLAESIL
                     TRGNRISEQEQLFLELKKCYPSSDIGLILILLMNLVQLTSGQALNISAGIPHAYISGN
                     IIECMTNSDNVVRIGLTNKYKDVAALLEILDFSPNEIQILNPDCREGISVYKSSAEEF
                     LVMNMKVAENFEQKIYTNDKVQIGLILNGRIELIWNSAEKLILKKGESVLLPACIKEY
                     VIRSQNWAEVYIASVN"
     gene            7673..>8156
                     /locus_tag="PLI55_08505"
     CDS             7673..>8156
                     /locus_tag="PLI55_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HPB00909.1"
                     /translation="MKKLTSIVVVIFLISILKAFSYGQGDSAAVNPNHPPIERDERLS
                     RRFPHSKNEMDFTEKRDILSERMEHHKGERAGAQKQSLSNDKEPEWVSHYALGLALAY
                     DVATDMAVDNIGNVYVTGYSPELPFGVDYFTAKYDASGNPIWSVRYDYNGDDFANAIA
                     V"
BASE COUNT         2420 a         1446 c         1705 g         2485 t
ORIGIN      
        1 tgccaacgac gattcgcata ttttctggta tgaaattgcc cccaatggta aaaacaatac
       61 tgtagaaatt gtttgcactc ccctcaatat tgcgaacgac attttcgaaa aggtctataa
      121 gaatgtttcc agtgcgatgc tgacctcggc gactttgcaa gttgccggag cttttgatta
      181 tgttcttagt cggaccggct tgaatttgat agaagatcat aatgttgtaa gtcgcgcgct
      241 cggttcacct ttttattata acgatcagat ggcttttttt acctatcacc agaacgataa
      301 tgaaattagt gaagcattta tagccgcgcg gttgataaaa cgcctttcca gcgaaaccgg
      361 gaagggcatt ctggttttgt tcacttctta taattttatg tacgaggttt atcaactatg
      421 tctgccgact tttatcgaaa tgggtgttac tcttttagcg caaggacagg gcgcatcgag
      481 gacagcgctt ctcgagcaat ttcgcttaga gcagaattcg gttttactgg gtaccgatag
      541 tttttgggaa ggagtggatg tggttggacc ggcattggaa attttagtta tcaccaagtt
      601 accgtttccg gtgcctaccg aaccgattat cgaagctaat gtcgaaaagt tgaaagccga
      661 ggggcaagat ccatttaacg aatactatgt cccggaatcg gtcttgaagt ttcgccaggg
      721 agttggtcgc ttaattcgct caattaatga tgtgggagtc gttattaatc ttgacagtcg
      781 cattgataag aaagcctatg gccgctactt caaaaaatcg ttaccggttg agccaatttc
      841 aattgttggc gaagatgaat tattagccgc tgtccgcaag ttttttagaa taggaaaatg
      901 aaatgaggat attccggatt tgtttatgtt ttctgctaag tacttcagta attttggcgg
      961 agaactccaa gaaattaacc gtgcgggaaa tcattgatga tccgggattg gatgcgagaa
     1021 cttaccggca gaaacaattt acctggactc cgaagggcga cggagtatta tttctcgaaa
     1081 aaaataaaaa tcaaaattgc gatttgctga aattttcgat taattccctt aaaattgaca
     1141 cggtttttac ttatgaacaa ttaaggtgga gtaacgatac tcactcgatt caacttgatc
     1201 cgttcggttt catatcggct cccgatggat tccgatattt actgactggt gagaatgacc
     1261 ttttcctagg cgatgaacgc gatggaacta tagtgcgttt gacaaatgac ggaacccaaa
     1321 aaagtgttcc aaaattctct cccaacggga aatgggtcag ctatatttgc gaccataata
     1381 tctggattgt caactgtgaa tcgagaagct cgctccagtt gacaaccgac ggcaatgaat
     1441 caatttttaa cggtgaatcc gactgggttt ataccgaaga gtttgatttt gaacaagcgt
     1501 atcagtggtc gccggacagt aaaatgattg cctattgtca gttaaatgaa aatggtgtca
     1561 gtcgctaccc gctggtagat tggtctccta tgcatccaga aatcgagtgg caatattatc
     1621 cgaatgcagg cgagaaaata ccagaagtca aagtattcac agttgatata gagacccaaa
     1681 aaattgtttg tatgaacaat tttcaatcca agaatgaata cattgtccgg atagattggt
     1741 tggctgacac cagtttggtg gttattcaat caattaatcg gcttcaaaat aaccagaaag
     1801 ttacctatgg taatccgcga accggccaga caaaagttat tctcgaagaa aaagatccgt
     1861 attggttaaa tattacagac ttatactatt ttatgaaaaa ttcgcgcaat tttatctatt
     1921 attcggaaat agacaatttc atgcatcttt atctctatga ttttgaaggc aatttgatca
     1981 aaccgattac tagcggcgat tggatggtta ccgaattgaa tggcgtgaac gaaaaagaca
     2041 gtctggttta tttcacagcg actaaaaatt cggtactcga acgacatatt tatagcgtgc
     2101 atattggctc cggtgccatt agccagattg ataccggtga tggagtgcac tcggcagttt
     2161 tttcaccgga tatgaaatat tatcttgatt ttttctcaaa tgttaactcg caagtagatg
     2221 tcagaattat tcaaaataat ggcagaaaaa ttacggactt atatcataat aatgatttca
     2281 gtccgcaaaa atataatttc ggcgttacgc gttttatcaa gataccggca attgatgggg
     2341 attctcttta tgctagccta ttatatccga gtgattttga tccgcaaaaa aaatatccgg
     2401 ttattgttta tgtctatggt ggtcctggag tgcaagtcgt tcaaaaccgt tatggaggtg
     2461 cctggaccca attgttagca caacagggtt atttaatttt cagcctcgac aatcgcggtt
     2521 cgtatgggcg tggacgatat tgggaacgca ggatttacca gcaactgggg aaaatagaat
     2581 tacaggatca gctggatggg gtgaaatatt taaaaagtct gccgtatatt gatccaggaa
     2641 gaatcggaat ctggggcgct agttatggtg ggtatatgac cttgtatgcc cttaccaaaa
     2701 cgctagaggt ttttaaaacc gggattgcta tttctccggt aacggattgg aaatattatg
     2761 atgccccata tactgagcga tatttaggat tgcccattaa tagtcaaaat atttataaag
     2821 aaagtgcacc gcttaattat gttaatcaga taaaagctaa ttttttacta attcatggca
     2881 cttatgatga taatgtccac ttacagcaaa gtctggaatt tattgatgaa ctaatcagca
     2941 acaatgtccg ttttgagttg atgctttatc ctggacgaaa acatggaata accgaccgtg
     3001 aagggcaaat tcatatgtac gagactttcc ttgagttcct tcagagaaat ctttgaccga
     3061 tttaggtaat gatgtcagct aatcggaaaa tatcggattc tctaaaatta tggttaagag
     3121 catatcgggc tccttttctg actgccagcg ttgcgccggt gattttaggt acttctgtag
     3181 gttggtatga gaacggtaga attaattttg tttccggttt actggcttta ttaggaacga
     3241 tttttgccca ctttggtgca aatattttta atgattatta cgatcacctg acgggctgtg
     3301 atgacaataa tctcgatttt actccattct ctggtggaag tcgcacgatc cagaacggcc
     3361 tattgacggc acgacaggca ttttgggctg gtttagtagc attcttgttg gtagttctaa
     3421 ttggattatt actaattgtc tatactggtc gcccggcttt aattattttt ggaatagtcg
     3481 gattggcaat cggtttttta tacactgcga ttcccgtcaa gttggcatat cgcggtctgg
     3541 gagaaatggg gatatttatt gcgtttggtc cattgatagt aataggagca tcctatgtcc
     3601 agactggcac aatttccttt ttagcgttct tagcttcggt accggctgga ttattggtga
     3661 tgttgattct atatgccaat gaaattctgg atgagaaagc cgaccgctcg gctgataaga
     3721 atacaattat cgtaaaaatc gcgaatcgta atacggctct taaaatatat cgtttgattt
     3781 tatatgtggt gccgatttgg attatagttt ttgtaattgt cggatatttt ccatactggt
     3841 cgttgattat tctctgcctt atgcctaaat acattggaac tatttttctc tcgaaaaagc
     3901 cgttcaatga cgttaaagag atggttccgc tcagtgctaa tacgattcag ctccaatttc
     3961 tgatgactat tacgctggcg atttccttta tattggctgg aatcaccggt tgatttattt
     4021 gctcatttcg tcgtttgggg tgtaaattta gtccgctttt tgtaaaaatt gggaagtcgt
     4081 ccaacggcag gacatcagac tctggatctg agtatcgggg ttcgaatccc tgcttcccag
     4141 cgaaagccgc ctcgggcggt tttttgaaat ggcgcattcg tctagtggcc taggacgctg
     4201 gcctctcacg tcggtaacag gggttcaaat cccctatgcg ctacaaatgt ggtcggttta
     4261 cgtannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnagattc ttgtgaatga
     4381 atattttaca aattatattt taactagtta ggttttgaaa aatgaaacaa taaatgtaaa
     4441 ggaggatttg ttatgtgtca aaagtgggcg tgttgtgtgt tttattatgg tggaattctt
     4501 agtatgctac tcggaatcct cgagaaattg tttggattta tgatatttga cctcaaaccg
     4561 tctcgcttcc tgatgtttgc cgccgtttgc gcactttatg caattgtcag cataatgtgc
     4621 cagccagtct gcgggaaaaa agagtagaat tagcctaagt cattatttat cacaaatatc
     4681 tcttcaataa tcttgaagag agaggatatt attaagtatt ttgcttcctg caatagttag
     4741 ttgatttaga aataaatcta atgttctaag aatgattgta ataaaatacg aataatctca
     4801 cattacccat tttatctgga tatatttatt cgatttttcg aatctggaaa agggctggaa
     4861 tcttggttca agtatttctg gctggggtgg aggtagtagt ggctagctgg atgttgaatt
     4921 tgatttgcca aatcattaag aggatagcgt agtcatcagt tggattttta gctatggtat
     4981 tattgacgat gcaagtttga ctggtttttt tcgttgatga tattttcgaa ataaccagct
     5041 gggtattata ccgctaagca gacggctgaa aatttcccat ccggaatcta tttttactaa
     5101 tggaatcctg ggaaatatag ccggactaga agatgtgttt tacagaaaaa taactgatat
     5161 tattcaacaa aagaagtcat tactttacta tatcagtagt tcttgtcttt caccagatag
     5221 atcatcggta ggctaatgat agttatagcg cctttcaaaa tcagattggc gaagaaaatt
     5281 gaccaaacga cagacggatt ataaatccca ccaaacgcgc cccatacaaa gattaggcta
     5341 tcaatcggaa tgctcaatga attacttacc aaaactcgtg cccactgatg acgtttggtg
     5401 actttattaa cccaaatgcg ataaatctcg gtatctaata attcggcaat gacttcggcg
     5461 attatgctgg caataacaat tcgccacacc ggcgaaagta ccttagcaaa ttctaattgt
     5521 ggacctacat tcggatcgcc gggtatataa gagactatcc aaaacaatag tgccattaat
     5581 agattaatca ccgccgcgca tataataact atacgtgtgc ctttcaatcc gatagtttta
     5641 tgcacaagat cacgcagggt aaaagtaaat ggatatatca gtgtaccggc atcgattgaa
     5701 aagcctaata aagaaattat tttcaaactt gtaatatccg acagcatttg agccgatata
     5761 taagcggcaa tgacgactat caccgaaatt ttgattgctt cattattcaa cgaagtttct
     5821 ttttccaagt gcacggttca atttaaaatt atttttctaa taggagaact attttttgtc
     5881 tgagtcgtaa aatagcgtga ttttattgat tatttctaaa tctaaacaat cataattgaa
     5941 aaataacttc ctcctcctca ctcgcaggtc tgctctccgc agaccttttt tattgaaaaa
     6001 tccattggta aaattgttac taaaacatta ttttagaaca tgatggaaaa taattttgag
     6061 attaaaccgt tcgttctgag aaataaaatt caacactatg aatggggaac gcgtggcgag
     6121 caggcattta tcccacgttt attaggaata cagtctgaag gacatcgtcc ctatgctgag
     6181 ctctggatcg gtgcccatcc gtcggcgcct tcacaggtaa atatttccaa tgaccttatt
     6241 gatttgactg aattgatcaa aaaacatcct gttgaaattc tcggtgagac cgtttttaaa
     6301 cggtttggag ccaatttgcc atttttattc aaagtcctat cagcaggcaa accactttct
     6361 attcaagcgc atcccaataa atcacaagct gttcgattac atcagcgtga cccaatccat
     6421 tatcccgatt ccaaccacaa attggaaata gcaatagcta tcgataatct taaagcttta
     6481 gtagggttgc taaatattgt tgaattaagg aaaaaactcg aattatatcc tccaatccaa
     6541 tcttttacag atagggtatt gccaggcgat agtacaattt cagaagcaga tgaaaccacc
     6601 atcgtcaaac agttttattc aaccttaatc cggaaatctg ttggcgaacc gcaattatac
     6661 ggaaatttag tcgaatccct tgcagaaagc attcttactc gtggtaatcg aatttcggaa
     6721 caggaacagc tgtttttaga attaaaaaaa tgctatccat cttccgatat cggtttgatt
     6781 ttaatattat taatgaattt ggtccaattg acctcgggac aggcgcttaa tatttcggca
     6841 ggtattccgc atgcctatat ttctggaaat ataattgagt gtatgactaa ttcagataac
     6901 gtcgtccgga ttggcttgac aaataaatat aaagatgttg ccgctctgct ggaaattctc
     6961 gatttttcac ctaatgagat tcaaatctta aatccagatt gccgggaggg aattagtgtt
     7021 tataaatcct cagctgaaga atttttagta atgaatatga aagtcgcaga aaattttgag
     7081 caaaaaattt ataccaacga caaagttcaa attggattaa ttctaaacgg tagaatagaa
     7141 ttaatttgga attcggcaga aaagttaata ttgaagaaag gggagagtgt tctcttaccg
     7201 gcttgtatta aggagtacgt tattcgctca caaaattggg ctgaagttta tattgcttct
     7261 gtcaattaga taattaaata ccgcatattt taggacaaga cattctttca taagaatata
     7321 tggagtgcac cgacccccgc cacgctctgc tgcggggcag gcgcgtcggt gcatgcaatg
     7381 accccgccac gcttcgctgc gggatcattg caccccaaag aaagattaag gctgaggtta
     7441 agtattcatt acagagcaga gcactatagc gaggggaaaa tagtagaatt aaataccgca
     7501 atattttagg acaagacatt ctttcaaatt caggaaaagg agttgaatca gaaataaata
     7561 tcaattatat tcttaacagg tgagaaataa tagttagatg aagattaatc atttaaacat
     7621 aatcctggac aaataaaaag ggaagaaaat taaataaaag gaggagttaa aaatgaagaa
     7681 gttaacctca atcgtagttg taattttttt gatttcaatc ttaaaggctt tttcttatgg
     7741 gcaaggggat tcagcagcag taaatcccaa ccacccgcct atcgagaggg atgagcggct
     7801 gagtaggaga tttccgcatt cgaagaatga aatggatttc actgagaaac gggatattct
     7861 ttcagaaaga atggagcatc ataagggaga aagagctgga gcccaaaagc aaagtctctc
     7921 taatgacaaa gagcctgaat gggtaagcca ctacgcatta ggacttgccc tagcctatga
     7981 tgttgcgact gatatggcag ttgataatat tggtaatgtc tatgttacag gctatagccc
     8041 agaactaccc tttggagtag attactttac tgcgaaatat gatgcctcgg gtaacccaat
     8101 ctggtcagtt agatacgact ataatggaga tgattttgca aacgccattg ccgttg
//
LOCUS       DAOOMU010000095         8101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_10424_length_8101_cov_13.698857, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000095 DAOOMU010000000
VERSION     DAOOMU010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8101
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10424_length_8101_cov_13.698857"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            555..1589
                     /locus_tag="PLI55_08510"
     CDS             555..1589
                     /locus_tag="PLI55_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB00910.1"
                     /translation="MTATLEDVAKKSGYSVSTVSRVLNNKGKVNRIKDETADKIHKIA
                     QELNYVPNQLARGLRLRKTHAIGLVIPDISNPFFAHVSRIIQIESYKLGYTLIVCNTD
                     ENQDTEIEQIQLLRSKGVDGFIIMPVGLKSDHIQELIDDKIPLVLLDRCFDSIDTNSV
                     VVDNYTGSYKAVEYLIKNGHKRIAIIQGLPNTSTNNMRVKGYKNALIKYNIEIDENLI
                     VGNDYRRENGYIETKLLLKMSNPPTAIFSTSDLITLGTLDAIIEEKLNVPQDVSLISF
                     DDVDFAPYLFTPLTAIQQPRQIMGEMAVKLLYDDLKSKGRKEKTRIELKPVLNIRKSV
                     RNLNSVINPD"
     gene            1615..3006
                     /locus_tag="PLI55_08515"
     CDS             1615..3006
                     /locus_tag="PLI55_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPB00911.1"
                     /translation="MQFKNIGNQHSPQKCANVFTANLRGWSRYFLLSFCLLVFIGCGK
                     KEATKDDKITITFWHSFVASTIPALEDLIEQYETENPNVKIKAQYVPTGDALIQKLIT
                     AIQSKTAPDISWIHSDFLADLVDADAIYEMDHFIKSNNGLSKSEIEDIYPALLQYSSL
                     RGKIYCIPMEATNLALIYNVDMVKEAGYQDGRAPSNWEELEEYAVRLSKDFNNDGKFD
                     RIGFFLPIFPAAGPLSGWMVWQWIPYLWQAGGYIIAEDQSKVLYNSEAGVKALTLWKN
                     IYDKLNLRTFTTDYDVSFASKLVAMAMDGPWNLPRFNRMLKNLNWSFAPLPKGPVKQA
                     TICGGEYLVIFKQSEHPDEAWSFVKWIIRPDIQARWAMKSGYLPVRRSVSEIPEFKKY
                     LIENPNFKVFVDQMEVAQIQRSMDYGALQITRHMAEAIEEATLGGGDPKAALDKAAAK
                     SNKVLQSYWQNAK"
     gene            2996..3928
                     /locus_tag="PLI55_08520"
     CDS             2996..3928
                     /locus_tag="PLI55_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HPB00912.1"
                     /translation="MLNKSTRVKKTIINRRDGNSKKNILTALGFLSPTLVIFCAFVLF
                     PVFFSFYLSFHKWNMFSSGREFLGLENYRRLFSDPEFWMVLKNTFIYTLGTVPLNMVI
                     ALLTAIILNRRIAGKRFLRTAIFTPVVISPVAAALIWRWIYDPNFGLLNYLMDLVGLP
                     SVNWLNEPKAAMVALIIMGVWKTFGTNMVLFSAGLQGIPDHYYEAAEIDGAGWWSKFW
                     NITMPMLSPTTFFIMIMSMISSFQVFDIVYVLTSGGPLGATKVMVFYIYENAFKFFEM
                     GYASAAAYVFFAILAVLTVLQVKFMQTRAGYEAV"
     gene            3928..4770
                     /locus_tag="PLI55_08525"
     CDS             3928..4770
                     /locus_tag="PLI55_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009593380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB00913.1"
                     /translation="MDIDFRKSSHIVKDIILHVIIYALALATIAPFIWMLLTSVKDIG
                     DIFVYPPKWLPSKFHFENYARAFQAAPFGRYYLNSLIVACTVTLGQLITCSMAAYAFA
                     RLQFKGRDVLFYIFLGTMMIPYQVTMIPSFMVLYWLGWVDSYQAMIVPGLASAFGTFL
                     LRQFFLTIPKELEEAAYIDGCGKFRVLWQIIIPLSKPALATLAIFTFMGTFNDFIWAL
                     IVVNSDHLRTVQLGLAIFRDRYITDWDLLMAGSVMATLPILIVFFFAQKYFIKGITLS
                     GIKG"
     gene            4831..5796
                     /locus_tag="PLI55_08530"
     CDS             4831..5796
                     /locus_tag="PLI55_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00914.1"
                     /translation="MNYLFKPNHFISASSILILFLLCLLRYCGEKNTLAPYQGARPMN
                     NIIIEQETFTPKFTWLGGYGCAFGVNRGDQARLDSTLQWLIKVDGDNLHYPVKYGELP
                     EGVTDITNEYGGHFEALIEDSTYTFWIVKEAAWDAVVENAGKVILMAELDTVTTIANE
                     DTLWIDSYSHVQKSCWIDVYTNVDMASIKLRGGFAKITLIETDTSNSPVITWEIIDSS
                     VEDSLVAAVGICLGESYSPKKIVWEAWSVDTVGGQKVYGKNNVIGQPLVIGSPIPGTK
                     VFVEYPAKGLSRNKTYLFWLAGKDWNGVDHLRSTKYHSYITFNTW"
     gene            5830..7131
                     /locus_tag="PLI55_08535"
     CDS             5830..7131
                     /locus_tag="PLI55_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HPB00915.1"
                     /translation="MRKKIIFWTISMVLILSDFTKAGDASTAPITSAKSLALGGYNYA
                     GLDGITTTFQNPGKILSTPGFGLAMNIIDRAGQQQCQKGSRDIHRSYLFNDFSGSAGI
                     YWVPNQKFGLAMVYNRALDYSVRWPLALMFTENGITRLYGFQLKSRLIADAYSQVFGL
                     QVGKMSLGAAINIYQIYQQFEFPISNPEWQTNRTNPVYALNLEQEDNAIGWSLGFILP
                     ITTTIKVGGSIRSGVDFDLEGNAKTDCFYALDSLAKKSRLHSRFQMPMTGGIGILYKP
                     TDQLNLNIDLSSEFWGSTQSKLDFNYSDTAWSAIIAAAPRDSITGYWRSGMPFYDHNS
                     YVFAIGLEYSGENNLKYRVGYNYQTTPNSDHSYSLLLPNVNQHRISAGIGFLFNQFYL
                     DLSIAYSVGVAKDIKKSQDEYFYGKYDTKTIIPSINVEYQL"
     gene            7245..>8101
                     /locus_tag="PLI55_08540"
     CDS             7245..>8101
                     /locus_tag="PLI55_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00916.1"
                     /translation="MRKVNMCLLALLLLVAVQSVALSQEEIVRLELNVPKVSPADVVI
                     DGVMDESVWGNAAEVNLVTETGYNIWASSYGREIAEPEYDELYGRLLWTDDTLYVFMH
                     IDEFVNDTTDLYWGGQFLDPTDTSGHWASDQLFIGVSNRLGVESWDNWEGNPMAAPDG
                     PYHLMIMGDRVTFNDGYPVYVPELDSAVIFDPSTFIRSAAQWNKETGVWDVELAIYNP
                     NVKASASVGFNVGGSFSSTYSRETTGDSYGYYTWQPNVPNEPFADPGDLGWCATYIQQ
                     NSEYWALLN"
BASE COUNT         2394 a         1354 c         1817 g         2536 t
ORIGIN      
        1 ttaagacggt tgtggtaaag aataaaatcg tcagcatcgt cctaaaataa aatggcacgt
       61 tagttattgc ccacgaacca cgcgaaaagg cgcgggatat attatggatt tatcagcggt
      121 ttacctaaaa taaaccagta accaaaacca gctaacagta accatttacc acaatcaatt
      181 atgcaaaaaa gcatttatgc agttctatgt gtcatttagt attttttgtg gttaacactc
      241 attgccctga cgagtataag tttggctggt ggacttatca ttgataaaaa cttagcgctc
      301 ttagcggtta gaagaaaaac aatattaatc tgactcgact tattagcggc aggtagaaaa
      361 tcatccccag attgtcgcga tttaaaggta tgtaacaata aatttaaaat actatatatt
      421 gaatgatatc aatgatttat tagatgaacg tagaaaaatt tcgtaatact ctgaaaaatt
      481 agcttgacaa atattttttt ctttactata cttattgcga aaatgattgc gcagatttac
      541 aaagtggttt caaaatgaca gcgaccctcg aagatgttgc caaaaaatca ggatattccg
      601 tttcaacagt ttctcgggta ttgaataata aagggaaagt aaaccggatt aaagatgaaa
      661 ctgccgataa aattcacaaa attgctcaag aactcaatta tgtcccgaat caattagccc
      721 gtggtttaag actgcggaag acgcatgcta ttggattggt aattccagat atttcaaacc
      781 ccttctttgc tcatgtttcg cgcattattc aaattgaatc ttataaactt ggttatactt
      841 tgattgtgtg caatactgat gaaaatcaag atactgaaat tgaacaaata caattactgc
      901 gtagcaaggg agtagatggt tttattataa tgccggtggg gttaaaatca gatcatatcc
      961 aggaactcat tgatgataaa attcctttgg ttttactgga taggtgtttt gattccattg
     1021 ataccaattc tgtggttgta gataactata ctggttccta taaagctgtt gaatatttaa
     1081 tcaaaaatgg tcataaaagg atcgcaatta tacaagggct tcccaatact tctactaaca
     1141 atatgcgggt taaaggttat aaaaatgccc ttattaaata taatatcgaa atcgatgaga
     1201 atttaatcgt tggtaacgat tatcgaagag aaaatggata tattgagacc aaactactat
     1261 tgaaaatgtc caatccacct acggcgatat tcagtacaag cgatttaatt actttgggta
     1321 ccttagatgc aattatcgaa gaaaaattga atgtccctca agatgtttcg ctgatttctt
     1381 ttgatgatgt agattttgcc ccgtatcttt ttaccccgct gacagctatt cagcaacctc
     1441 ggcagatcat gggtgagatg gcggttaaat tgttgtatga cgatttaaaa agtaagggaa
     1501 gaaaagagaa aaccaggatc gaattaaaac ctgtcctcaa tattcgaaaa tccgtccgaa
     1561 atctaaattc tgttattaat cccgattaaa accaataaaa attaggtccg aactatgcaa
     1621 ttcaaaaata tcggcaatca acactcaccc cagaaatgcg caaacgtttt tacagccaat
     1681 ctgaggggat ggagtagata ttttttacta tcgttttgct tattagtatt tattggatgt
     1741 gggaaaaaag aagctactaa agatgataaa atcaccatca ctttctggca tagcttcgtc
     1801 gcatctacta taccagcgct cgaagattta atcgaacaat atgaaactga aaatcccaat
     1861 gtcaaaataa aagctcaata tgtgccaact ggtgatgctc taattcaaaa acttatcacg
     1921 gcaattcaga gtaagaccgc gcctgatatt tcctggattc actctgattt tctggctgat
     1981 ttagttgacg cggatgcgat ttatgagatg gatcatttta tcaaaagcaa caatggactt
     2041 agtaaatccg aaatagaaga tatctatcct gctttattac aatattcttc tctgcggggg
     2101 aagatatatt gtattccaat ggaagccact aacctggcgt tgatatataa tgttgatatg
     2161 gttaaagaag ctggctatca ggatggtcgt gctccctcga attgggaaga gttagaagaa
     2221 tatgccgtgc gattatctaa agattttaat aatgacggga aatttgaccg gatcggattt
     2281 tttctgccca tatttccagc cgcgggccct ctaagtggct ggatggtttg gcagtggatt
     2341 ccctatttat ggcaagcggg tggttatatt atagctgaag accaatctaa agtattatat
     2401 aatagtgaag cgggagttaa ggctctgaca ctctggaaaa acatatatga caaattgaat
     2461 ttacggacat ttactacgga ttacgatgta tcttttgcct cgaagttggt ggctatggcg
     2521 atggatggac cttggaacct cccgcgattt aatcgaatgc tgaaaaattt gaattggtcg
     2581 tttgcaccgt tgcccaaagg tcctgtaaaa caggcgacca tctgcggtgg cgaatatctg
     2641 gttattttca aacaatctga acatcccgat gaagcctggt catttgtgaa gtggatcatt
     2701 cggcctgata ttcaagctcg ctgggctatg aaatccggtt atttgccggt gcggcggtca
     2761 gtatcagaaa taccggagtt caagaaatat ctaatagaaa acccgaattt taaagtattt
     2821 gtagaccaga tggaagttgc ccagattcag cgctctatgg attatggggc tttacaaatt
     2881 actcgacata tggcggaagc cattgaagaa gcaacacttg gtggcggtga tccaaaggca
     2941 gctctggata aagcggccgc gaaatctaat aaggtcttac aatcgtattg gcagaatgct
     3001 aaataaatct accagagtta aaaagacgat tataaatcgc cgggatggga atagtaaaaa
     3061 gaatattctc acagcgctcg gatttttatc tccgacgttg gttattttct gcgcttttgt
     3121 tttattcccg gtattcttct cgttttatct cagttttcat aaatggaata tgttcagtag
     3181 tgggcgggaa ttccttggtt tagagaacta tcgtcggtta ttttctgatc ctgaattctg
     3241 gatggttctg aaaaatactt ttatttacac acttggtacg gttcctttaa atatggtaat
     3301 tgctctgtta actgctatta tacttaaccg acgcatcgca ggaaaacggt ttttacgtac
     3361 ggcgattttc actcctgtgg ttatatcccc ggtagcggct gcgttaatct ggcgttggat
     3421 ttatgacccc aattttggtt tgttaaacta tttaatggat ctcgtggggc ttccctcggt
     3481 aaattggctc aacgaaccga aagccgctat ggttgctctt attatcatgg gagtgtggaa
     3541 aacatttggg acaaatatgg ttttattttc cgccggatta caagggattc ccgatcatta
     3601 ctatgaagcc gccgaaattg atggcgccgg atggtggtcg aaattttgga atattacaat
     3661 gccgatgtta agtcctacca catttttcat tatgataatg tcaatgatca gctcgtttca
     3721 ggtctttgat attgtctatg ttttgacttc agggggacct ctgggcgcaa cgaaggtgat
     3781 ggtattttat atttatgaga atgccttcaa atttttcgag atggggtatg cctctgcggc
     3841 ggcttatgtt ttctttgcaa tattagcggt gctaacggta ttacaagtaa agtttatgca
     3901 aacccgtgcc ggttatgagg cggtataatg gatatagatt ttcggaaaag tagtcatata
     3961 gtaaaagata ttatccttca tgttattata tacgctttag ctttagcgac aattgcacct
     4021 tttatctgga tgctcctaac ttcggtaaaa gatattgggg atatatttgt gtatccacca
     4081 aaatggctac cttcaaagtt ccattttgag aattatgctc gggcttttca ggcggctccc
     4141 tttgggcgat attatttaaa tagtctcata gtagcttgta ctgtgacatt agggcagcta
     4201 attacttgtt ctatggcggc ttatgctttt gctcggttac aattcaaagg acgggatgtt
     4261 cttttctata tttttttagg aactatgatg attccttacc aggtaacgat gattcctagt
     4321 tttatggttt tatactggct cggatgggtt gacagctatc aagctatgat tgttccgggc
     4381 ttggcttcgg cgtttggcac atttttattg agacaatttt tcttgacgat tcctaaggaa
     4441 ttagaagagg cggcctatat tgatggctgt ggaaaattta gagtattgtg gcagataatt
     4501 attcctttat cgaaaccggc tctggcaaca ttagccattt ttacatttat ggggactttt
     4561 aatgatttta tctgggcttt aatcgtggtc aattcagatc atttacgcac ggttcagtta
     4621 ggactagcta tattccgcga ccgatatatt acagactggg atttattaat ggctggttcg
     4681 gtaatggcaa ctttaccaat tttaatcgtc tttttcttcg ctcaaaaata tttcataaaa
     4741 ggtatcactt taagtggaat taaaggatag tatcgtgatt aagaagaggc taataataat
     4801 tattagtaaa aacagcataa ggtttattct atgaattact tatttaaacc aaaccacttt
     4861 atttcggcaa gtagcatttt aattctattt ttactttgcc ttttaaggta ctgtggggag
     4921 aagaatacgc tggctccgta tcaaggcgcg cggccaatga acaatattat tattgaacag
     4981 gaaacattta cgcccaaatt tacttggttg ggtggatatg gatgtgcttt tggagtaaat
     5041 cgcggcgatc aagcacggct tgacagtact ttgcaatggt taataaaggt tgatggtgac
     5101 aatttgcact acccggttaa atatggcgaa ttgcccgaag gggtaactga tataacaaat
     5161 gaatatggtg gacattttga agcattaatt gaagacagca cctatacatt ttggattgta
     5221 aaggaagccg cttgggatgc cgtagtagaa aatgccggta aagtaatctt aatggcagaa
     5281 ctggacacgg taacgacgat tgccaatgag gatactttat ggatagacag ttacagtcat
     5341 gttcaaaaga gttgctggat agatgtttat acaaatgttg atatggcaag cattaagttg
     5401 aggggtggat ttgccaaaat cactcttatt gaaacggata ccagcaattc accggtcata
     5461 acctgggaga ttattgattc aagcgttgaa gattctctcg tagcggcggt cggaatctgt
     5521 ttaggtgaga gctatagccc caaaaaaata gtctgggaag cctggtcggt ggataccgta
     5581 ggtggtcaaa aggtatatgg aaaaaacaat gtaataggtc agccgctggt aataggaagc
     5641 ccgattcccg ggacgaaggt atttgttgaa tatcctgcaa aaggtttgtc ccgcaataag
     5701 acctatttat tctggctggc tggtaaggat tggaatgggg ttgatcactt aagatccact
     5761 aagtatcatt catatataac ctttaatact tggtgatttg aaagagtttt attgaagagg
     5821 aaattctaag tgagaaaaaa aattatcttt tggacaattt caatggtttt gattctttcg
     5881 gattttacaa aagccggaga tgcgagtact gctccaataa ccagtgcaaa atccctagct
     5941 ttggggggtt ataattatgc cggattagat ggtataacaa ctacgtttca aaatccgggt
     6001 aaaattttaa gcacaccggg ttttggtctg gcgatgaata tcattgatcg agctgggcaa
     6061 caacaatgtc aaaaaggaag tcgtgatatc catcgttcct atttattcaa tgatttcagc
     6121 ggttcagcgg gtatttactg ggtgcctaac caaaagtttg ggctggctat ggtctataat
     6181 cgagcccttg attattcggt tcgatggcca ctggcattaa tgtttactga aaatggaata
     6241 acccgtttgt atggctttca attaaaatca cgtctgattg cggatgccta ttcgcaggtt
     6301 ttcggattac aagtcgggaa aatgtcattg ggtgccgcga ttaatattta tcagatttac
     6361 caacaatttg agtttccgat ttcgaatcct gagtggcaga cgaacaggac taatccggtc
     6421 tacgctttga atttagaaca agaggataat gccataggtt ggagcctagg atttatttta
     6481 cctataacaa cgacgattaa ggtaggcggg agcattcgga gcggcgtaga ttttgacctt
     6541 gaaggtaatg ctaaaacgga ttgcttttat gcgcttgata gtctggcgaa gaaatcgcga
     6601 cttcacagtc gttttcaaat gccgatgact ggtggtatag gaattttata caaacccacc
     6661 gatcaactaa atctaaatat tgatctatcc agcgaattct ggggcagcac tcaatccaaa
     6721 ctggatttta attatagtga cactgcctgg tctgccataa tagctgcggc tccgcgtgat
     6781 agtatcaccg gttactggcg ttcgggaatg ccgttttacg atcataatag ttatgtattt
     6841 gctatcggat tggaatattc aggcgagaat aatttgaagt accgggttgg atataattat
     6901 cagacaaccc cgaattcaga tcatagttat agtcttttat tgccgaatgt aaatcaacat
     6961 agaatatcgg ctggtattgg atttttattt aaccaatttt atctagatct cagtattgct
     7021 tattctgtgg gagttgctaa agatatcaag aaaagccagg atgaatattt ttatggtaaa
     7081 tatgatacta aaacaattat tccatcaata aacgttgagt atcaattatg agaaaggtaa
     7141 aaaaacaaat gaaaggaggt gtaatttata agtcaggctt actttatttt tgtaaaacag
     7201 gacaaggaat tattaaccaa tttattaacc aaatggaggt aagtatgcgt aaggttaata
     7261 tgtgtttgtt agcattattg ctattagtgg cggtacaatc ggtggcatta agccaagaag
     7321 agattgttcg tttagagttg aatgttccga aggtatcacc tgcggatgta gtgattgatg
     7381 gagtaatgga tgagtcggtt tggggtaacg cggcggaagt gaatttagtg actgagacgg
     7441 gttacaatat ctgggcgagt tcttatggtc gtgagatagc cgagccggag tatgatgagt
     7501 tgtatgggcg tttgttgtgg acggatgata ctctatatgt atttatgcat attgatgagt
     7561 ttgtcaatga cacgacggat ttgtactggg gtggtcaatt tttggatccg actgacacat
     7621 ctggacattg ggcgagtgac cagttattta taggggtttc gaaccgttta ggagtggaat
     7681 cttgggataa ctgggaaggg aatccgatgg cggcaccgga tggtccttat catttgatga
     7741 taatgggaga tcgggtaaca tttaatgatg gttatccggt atatgtacct gaattagact
     7801 cggcggtaat atttgatcct tcgacgttta tacgatcggc ggcgcagtgg aataaggaga
     7861 ccggagtatg ggatgtagag ttagcgattt acaatcccaa tgttaaggca agtgcttcgg
     7921 ttggatttaa tgttggtggt agtttttctt cgacgtattc tcgtgagacg acgggtgatt
     7981 cgtatggata ttacacctgg cagccgaatg tgccgaatga accgtttgca gatccgggag
     8041 atctgggttg gtgtgccact tatattcagc aaaattctga atactgggct ctgctgaatt
     8101 t
//
LOCUS       DAOOMU010000096         8092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_10438_length_8092_cov_17.762100, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000096 DAOOMU010000000
VERSION     DAOOMU010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8092
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10438_length_8092_cov_17.762100"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1062
                     /gene="trpS"
                     /locus_tag="PLI55_08545"
     CDS             73..1062
                     /gene="trpS"
                     /locus_tag="PLI55_08545"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPB00917.1"
                     /translation="MENRKVKGRILSGMRPTGKMHLGNYVGALENWVKLQDEYENYHL
                     IADYHALTTNLDTSELFSNTIEMVYDWLAVGIDPQRSPIFRQSQVKEHCELFLIFAML
                     ITTARLERNPTLKEQVRDLNIGTLSFGHLGYPVLQAADILIYKANLVPVGEDQVPHVE
                     ITREIARKFNQTWGKVFPIPEAKLTKFARLPGLDGNKMSKSVGNTILLADSDEEINSK
                     IKKAITDPQKIRRGDPGRPEICLVYTYHQKFNPAQCPEIYAGCTSGALGCVECKMKCG
                     KAIQSVIAPYRTHRMYYATHQSEVIDILIDGEKRARQVAQNTMADVHNKMRIG"
     gene            1116..1856
                     /locus_tag="PLI55_08550"
     CDS             1116..1856
                     /locus_tag="PLI55_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPB00918.1"
                     /translation="MSYNVKLNVYEGPLDLLLFFIKRDEINIYDIPIAYITDEYLNYL
                     QLMQSLNLQLAGEFILMASILIRIKVQMLLPRLETEKLEDIEDPRTELVQMLVEYQKY
                     KEIGEQLKRLEANQSRHFPVTLTSDQRNYDPELFLSDITLIELGLTFQQILEKMPKVE
                     PVNLEIMKVNIRQQILFIRECLKKRSRIRFSEIAAKLTDRLEVVVTFLAILEMIKSME
                     INVSQKDIFGEIWITKKKTIQDNVISNA"
     gene            1837..2397
                     /gene="scpB"
                     /locus_tag="PLI55_08555"
     CDS             1837..2397
                     /gene="scpB"
                     /locus_tag="PLI55_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPB00919.1"
                     /translation="MLSQTHRIVLALLFGATEPLTKSQVDICLEEDIDLTQIIADLNQ
                     YLETEGLPVWIQLVAGGYRLMTRAEYEPWLSRLFQNKGKLRLSRSALETLAIIAYKQP
                     VTKSEIDAIRGVDTSLRPLVEKNLIKITGRQEGPGRPLLYGTTNHFLEYFGLASLQDL
                     PQVKELAEIAGEDNLQSDIVEQDATE"
     gene            2384..3097
                     /locus_tag="PLI55_08560"
     CDS             2384..3097
                     /locus_tag="PLI55_08560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPB00920.1"
                     /translation="MRLNRYLAQGGFGSRRKCEDLIRGGRVKINGCIVTNLATQVKET
                     DQVTVDNRRIVPPKPVYILLNKPAGYITTMHEQFGRKAVTDLVNNAFAKPIGRLDMAT
                     TGVLLLTNDGELAYRLTHPKFGVERIYRATIAGRVSPDIKKQVAEGISIGGAQKASGQ
                     ILTIKYLDGFSSVTIELKEGQNREVRRIFAVLGYKVISLDRLSFAGVRYSGLERGQWR
                     NLNESEVRRLKTLVGLADQ"
     gene            3221..3940
                     /gene="cmk"
                     /locus_tag="PLI55_08565"
     CDS             3221..3940
                     /gene="cmk"
                     /locus_tag="PLI55_08565"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPB00921.1"
                     /translation="MGIIIAIDGPAGSGKSTTAREVARRLNFTYIDTGAMYRAITLAV
                     IRQNVAVEDHQSVIKIAEQADIQFEWADGNLRTILNGEDVSNEIRSAQVARLVSPVSA
                     IGGVRDILVKRQREMAKKANIVMEGRDIGTNVFPDADFKFYLEADLETRARRRINDYQ
                     KIGQNLSITEIMTEIEQRDKIDSSREHSPLKKAPDAIVIDTSNLKFEEQVEMIIQIVK
                     SSELFKKIEKAPVINGTNCKN"
     gene            3965..>6023
                     /gene="rpsA"
                     /locus_tag="PLI55_08570"
     CDS             3965..>6023
                     /gene="rpsA"
                     /locus_tag="PLI55_08570"
                     /inference="COORDINATES: protein motif:HMM:NF004953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPB00922.1"
                     /translation="MLEEEQTLNPKPSEESEVIPDINPADPFAEPVTARDATIEERTS
                     ATSDYTTELVTSGPLSEIVRVYDLKDIDKLNEVQAPKAEDHTKIYENSLKDIAKDQRI
                     LGTVVAINENEILVDVGFKSEGIITREEFEEGELPKIGDQIEVFVDVLEDENGQMILS
                     KRKADFMRIWERIKEIYAAGETVEGQIVSRIKGGMVVDIMGIDAFLPGSQIDIRPVTD
                     FDSYIGKTFEFRIVKLNELRKNIVLSRKELLEESMKEKRDDLLSKIKVGDILTGRVKN
                     ITDFGVFIDLGGLDGLLHITDISWGRINHPREVVQMDQELTVKVIDYDQEKQRVSLGL
                     KQLTTHPWEGIESKFPVGTIVKGKVVNITNYGVFVELEKGVEGLIHISEISWTQHIKH
                     PSEVFKLGDEIEAKVLGIDSAERKISLGYKQLEPDPWESIEQKFTVGSVHKGIVRNLT
                     PYGAFIELQEGVDGFAHISDMSWTKKIRHPRELLKKDDEVEFKILEISKENRKISLGL
                     KQVTEDPWPMIETLFSVGSIVEGEVAKVTDKFIVVNLEHDLEGIIPLGQMPKKDRKDL
                     SKAVEIGEKLQLKVLEVNRQEKKIILSREQAIPREPKTEVEAYMMQQAETTNKLEIPS
                     EIIQKIAESEEKTQEKEEKSAAKVKSLAKKAKKAEKVEKPEDLTPEEEKPKKKSTKKV
                     ISEE"
     assembly_gap    6024..6123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6124..7095
                     /locus_tag="PLI55_08575"
     CDS             <6124..7095
                     /locus_tag="PLI55_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPB00923.1"
                     /translation="QAVRKWITTNTRTKIWIFVFTVFLWLYVVLNNTYSYTFSAKLEV
                     RNIDPTKTTIEKLPSQVQANFSGKGIDLFYLILSPQQSIKFVVDCYSIKQYYDFPLNE
                     YFKNNPEKVITPRNINVKLDHIIWPETLRVVLDDLVTAKVPVKPEVEIQLAPGYVLAG
                     SMRIIPDSVTLRGPRTYVRQYTAVPTKKLVLTNVSSSISQEIDLEFVPKYNVYIDVTR
                     VRIQQKVDQLGEKKLTDIPVRVINLAQDQKAEIIPPTATLTISGGIEKLKTVQPEDIK
                     IVFDFATDWRPDESFYTPTVELPPGLLNWWNLEPDTFEIRIIRERQP"
     gene            7096..>8092
                     /gene="tsaD"
                     /locus_tag="PLI55_08580"
     CDS             7096..>8092
                     /gene="tsaD"
                     /locus_tag="PLI55_08580"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPB00924.1"
                     /translation="MLILGIETSCDETAVAILNDNEILVNLVSSQLLHQQFGGVVPEF
                     ASREHMRLLDQMVKEAFVQTGLQPENLDGIAVTRGPGLMGALLVGLSFAKGLAYSLRK
                     PLLGVNHIEGHIMANFLTCPDLKFPFLCLLVSGGHTQIIQVNDFGNYQILGHSVDDAA
                     GEAFDKAARILELPYPGGPQIDQMARRGNPNFYQFPRAKVRSEQYDFSFSGLKTAMLY
                     LVRQRGQKWAQENLADLCAAYQEAIVDVLKINTFTAVKNLHANKLVLAGGVAANSRLR
                     QVFQEQADYYQIELIIPSLKYCTDNAAMIARAGQEMLSRNQISPFSISAVPNLTLT"
BASE COUNT         2541 a         1628 c         1702 g         2121 t
ORIGIN      
        1 atacattaca acgatttgcc gaatcaccaa ttgacgagtg gcggccatat ttatttttag
       61 aataaggaca aaatggaaaa tcgcaaagta aaaggcagaa tcttaagcgg aatgcgcccg
      121 accggtaaaa tgcatttagg taattacgtc ggagctctcg aaaactgggt aaaattacaa
      181 gacgaatacg aaaactatca tctcatcgcc gattatcacg cgctcacgac taatctggat
      241 acaagtgaat tattctcgaa cacgattgag atggtttacg attggctggc agtcggaata
      301 gatcctcaac gcagtccgat ttttcgtcag agtcaggtca aagaacactg cgaactattt
      361 ctaatattcg caatgttaat taccacagcc cgccttgaac gtaaccccac ccttaaagaa
      421 caagtgcgtg atttaaatat tggcactttg tcgttcggac atctcggtta ccccgtgctc
      481 caagccgccg atattttgat atataaagct aaccttgtgc cggttggcga agatcaggtg
      541 ccccatgtag aaattacacg ggaaatcgcc cggaaattca atcagacctg gggtaaagtt
      601 ttccctatac cggaagccaa actaaccaaa ttcgcccgcc ttcccggttt agacggcaat
      661 aaaatgagta aatccgtcgg caatacgatc ctcttagcgg attcggatga agaaatcaat
      721 tccaaaatta aaaaagctat cactgatccc cagaaaataa gacgcggcga tcccggtcgt
      781 cccgaaattt gtctggttta tacctatcat caaaaattta atcctgccca atgtcccgaa
      841 atttacgccg gttgcacaag cggcgctctc ggttgtgtcg aatgtaaaat gaaatgcggg
      901 aaggcgattc aaagtgttat cgcaccttat cgtactcatc ggatgtatta tgccactcac
      961 caatctgagg tcattgacat tttaattgat ggcgagaaac gggctcgtca ggttgcccaa
     1021 aacactatgg ctgatgtcca taataaaatg agaatcggat aagacgtgat aatccattac
     1081 ctggactggc gaatccagca attttaatcg gattaatgag ttacaacgtc aaactaaacg
     1141 tatatgaagg gccactcgac ctcttgcttt tctttattaa gagagatgag attaacattt
     1201 acgacatacc cattgcctat ataacggatg aatatcttaa ttatctacaa ctcatgcaga
     1261 gtttaaatct gcagttagcg ggtgaattta tcctgatggc ttccatcctt ataagaatca
     1321 aagttcaaat gctcctgccg cgtttggaaa ctgaaaaact cgaggatatt gaagatccac
     1381 gcaccgaatt agtccaaatg ctcgtcgaat atcagaaata caaagagatc ggcgagcaac
     1441 tgaagcgact tgaagcaaat caatcccgcc attttccagt cacactgacg tcagaccagc
     1501 gcaattatga tcctgaactt ttcctaagtg atataacttt gatcgagcta gggctcacct
     1561 ttcagcaaat cctcgagaaa atgccaaaag ttgagccggt caatctcgaa attatgaagg
     1621 tcaacattcg tcagcaaata ttattcataa gagaatgttt aaaaaaaaga agccgtattc
     1681 gattttccga aattgcggct aaattaactg accgactcga agttgtggtt acttttctgg
     1741 ctattctgga aatgatcaaa tcaatggaaa ttaacgtatc gcagaaggat attttcggtg
     1801 aaatatggat aaccaagaaa aaaactattc aggataatgt tatctcaaac gcatagaatc
     1861 gtactagcgc ttttattcgg cgcaaccgag ccgttgacta aatcacaggt agatatttgt
     1921 ctcgaagagg acattgattt gacacaaatt atcgccgatt taaatcaata tcttgaaacg
     1981 gaaggacttc cggtctggat tcagttggtg gcgggaggtt atcggctaat gacaagagct
     2041 gaatacgaac cctggctaag tcggcttttt caaaataagg gcaaattacg tctgtcacgc
     2101 tcggctctgg aaacgctagc tattattgct tacaaacaac cggttaccaa aagcgaaatt
     2161 gatgcgattc gcggggttga taccagtctc agacctcttg tagaaaaaaa tctgattaaa
     2221 attaccggtc gtcaagaagg acctgggcga cctctgctat atggtaccac taaccatttt
     2281 ttagaatatt tcggattggc gtctttgcaa gatttaccac aggttaaaga actggccgaa
     2341 attgccggtg aagacaatct ccaatctgat attgtcgaac aggatgcgac tgaatagata
     2401 tttagcgcag ggcggttttg gttcacgacg caaatgtgaa gatttgatcc gaggaggtcg
     2461 ggttaaaatt aacggatgta tcgtaaccaa cctggctact caggtaaaag aaaccgatca
     2521 agtgactgtt gacaatcgcc gaattgttcc acctaaacca gtgtatattc tcttaaacaa
     2581 accagctggc tatataacaa caatgcacga acaattcggc cgcaaagcag tcaccgattt
     2641 agtaaataat gccttcgcca aacctattgg tcgtttggat atggctacaa ccggcgtgct
     2701 tctgctgact aatgatggcg aattagccta ccggttgact caccccaagt ttggtgttga
     2761 acgcatttat cgagcaacaa ttgctggtag agtctctccc gatattaaaa aacaggtagc
     2821 ggaaggaatt tccatcggag gggcgcaaaa agcatctggt caaatcctta ctattaaata
     2881 tttagatggt ttttccagcg tcaccatcga attaaaggaa ggtcagaatc gcgaagtgcg
     2941 tcgcattttt gctgttttag gctataaagt catttcactg gatcggctaa gtttcgccgg
     3001 tgtgcgctat tcaggattgg aacgtggtca atggcgcaat ttaaatgaat ctgaagtacg
     3061 gcgactgaag actctggttg gtttggcaga tcaatgacgg agtgacaaat ttttacaatt
     3121 gattattcaa atattgtatt aatttacctt aagatagctg aaaggaattt gaattgttat
     3181 cctatggaat taaatttacg cgctttttga aaggtagaat atgggcatta taatagcaat
     3241 cgatggaccg gccggtagcg gaaaaagtac cactgcgcgg gaagtagctc gccgactgaa
     3301 tttcacttat attgataccg gcgcaatgta ccgagcgata actttggcag ttatccggca
     3361 aaatgttgca gtcgaagatc atcaatctgt gataaaaatt gccgaacaag ccgacattca
     3421 gtttgagtgg gcggatggca atttgcgtac gattttaaac ggtgaggatg taagtaatga
     3481 aatccgttca gctcaagtcg cgcgtttggt aagtccggtt agtgccattg gaggcgtacg
     3541 tgacatttta gtcaagcgcc agcgcgaaat ggctaagaag gcaaatattg ttatggaagg
     3601 tcgcgatatc ggcaccaatg ttttccccga tgctgatttt aaattttatt tagaagctga
     3661 tttggaaacg agagctcgcc gacgaattaa cgactatcaa aagatcggcc agaatttatc
     3721 tataacggaa attatgactg agatcgaaca aagagataaa atcgattctt cgcgcgaaca
     3781 ttcacctctt aaaaaagccc ctgatgctat tgtaattgac acctccaatt taaaatttga
     3841 agaacaggtt gagatgatta tacaaattgt gaaatcatct gaactcttca aaaagataga
     3901 aaaggctccg gtcattaacg gaaccaattg taaaaattaa ccctaaacca aaggaacatt
     3961 ttcaatgtta gaagaagaac aaaccttaaa ccctaaaccc tccgaagaat cggaagtaat
     4021 tccggacatt aacccggctg atccattcgc cgagccagtg actgccagag atgccacgat
     4081 tgaggaaaga acatccgcca cttcggatta tactaccgaa ttagtcactt cggggccatt
     4141 gtcagaaatt gtaagagtat atgatttaaa agatattgat aaattgaatg aagtccaggc
     4201 tcccaaggcc gaagaccata caaaaatcta tgaaaactca ctaaaagata ttgcaaaaga
     4261 tcagcgcata ctcggaacag ttgtagccat taatgaaaat gaaattttag ttgatgtcgg
     4321 cttcaaatcc gaaggtatta ttacccgtga ggaatttgaa gaaggggaat taccgaaaat
     4381 cggcgaccag attgaagtat ttgtagatgt acttgaagac gaaaatggtc agatgattct
     4441 ttccaagcgg aaagccgatt ttatgcgaat ctgggaacga attaaggaaa tttatgcggc
     4501 tggtgaaact gtagaagggc aaattgtcag ccgtattaaa ggtggaatgg tagtggatat
     4561 tatgggaatt gatgcctttc tgcccggctc acaaattgat attcgaccgg ttaccgattt
     4621 tgattcctat atcgggaaaa cttttgaatt tcggattgtc aaattaaatg aattacggaa
     4681 aaatattgtc ttgagccgta aagaattact cgaagaaagt atgaaggaaa aacgtgatga
     4741 tttattatcc aaaatcaaag tcggtgatat tctgactggc cgggttaaaa atataaccga
     4801 ttttggagtt tttattgatc tcggtggatt agacggctta cttcatatca ccgatatttc
     4861 ctggggacgt atcaatcacc cgagagaagt agttcagatg gatcaggaat tgacagtcaa
     4921 agtcattgat tacgatcaag aaaaacagcg tgtttcgctc ggactcaaac aactgaccac
     4981 ccatccctgg gaaggtattg aaagcaaatt tcctgttggg acgatcgtta aaggcaaagt
     5041 tgttaatatc actaattatg gcgtattcgt ggaactcgaa aaaggggttg aaggactaat
     5101 ccatatctcg gaaatatctt ggactcagca tattaaacat ccttcagagg tgttcaaatt
     5161 gggcgatgaa atcgaggcta aagtgctggg catagacagt gccgaacgga aaatttcatt
     5221 aggatataaa cagcttgaac cagacccgtg ggaatcaatt gaacagaaat tcacagtggg
     5281 ttctgttcac aaaggtatag ttcgaaatct gactccttat ggcgccttta tcgaacttca
     5341 agaaggtgtt gacggatttg cccatatttc agatatgtcc tggacgaaaa aaatccgcca
     5401 tccccgggaa ttgctaaaaa aagacgatga agtcgaattc aagattctgg aaatctcgaa
     5461 ggaaaatcgc aaaatctctc tcggtttaaa gcaggttact gaagatccct ggccaatgat
     5521 tgaaactctt ttttccgtcg gaagtatagt ggaaggcgaa gtcgctaaag tgactgataa
     5581 attcattgtg gtcaatctag aacacgatct cgaaggaata attccattag gacaaatgcc
     5641 taaaaaagat cgtaaagacc tcagcaaggc agttgaaatt ggtgaaaaac tgcagctcaa
     5701 agtcttggaa gtcaaccgac aagaaaagaa aatcatacta tcacgtgaac aggctattcc
     5761 gcgcgaaccc aagaccgaag tagaagctta tatgatgcaa caggctgaaa cgactaataa
     5821 actcgaaatc ccgagtgaaa taattcagaa aatcgccgaa tcggaagaaa aaactcagga
     5881 aaaagaagaa aagtcagccg caaaggttaa atctctcgcc aaaaaagcca agaaagcgga
     5941 aaaggtcgaa aaaccagaag atctcacccc agaagaagaa aaacccaaga aaaaaagtac
     6001 caaaaaagtc atttctgaag aaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnncaagccg tccgaaaatg gattaccacc aacacgcgta ctaagatttg gatcttcgtt
     6181 ttcaccgttt ttctctggct ctacgtcgta ttgaataata cttattctta tactttttca
     6241 gctaaactgg aggtgagaaa tattgacccg actaaaacga cgattgagaa attaccgtcg
     6301 caagtccaag ctaatttttc aggtaaagga attgacctgt tttatcttat cctatcacct
     6361 caacaatcaa tcaaatttgt cgtagattgc tactccatta aacaatatta cgatttccct
     6421 ttaaatgaat actttaaaaa taatcccgaa aaagtcatca ctccacgaaa tattaatgtc
     6481 aaactcgatc acattatctg gccggaaacc ttgcgcgttg ttttggacga tttagtcacg
     6541 gcaaaagtac cggtcaaacc ggaggttgaa atacaattag cacctggcta tgtcttagcc
     6601 ggttcaatgc gtataattcc agacagcgtt accttaagag gtccgcgaac ttatgtcagg
     6661 caatatactg cagtgccaac taaaaagcta gttctcacta atgtttccag ttcgatttcg
     6721 caggaaattg atttagaatt tgtccctaag tataatgttt atattgatgt aacgcgcgtc
     6781 cgcattcagc agaaggtcga ccaattgggt gaaaaaaaat taaccgacat cccagttaga
     6841 gtaatcaatc ttgctcaaga ccagaaagcc gaaattattc ctccaacagc cacattgact
     6901 atttccggtg gaatcgaaaa attaaaaaca gttcagccgg aagatattaa aatcgtattt
     6961 gattttgcca cagattggcg accggatgag tccttttaca ctccaaccgt agaactgccg
     7021 cccggactct taaactggtg gaatctcgaa cccgatactt ttgaaattcg gataattcgc
     7081 gagaggcaac cctgaatgct aattctcggc atcgaaacat cctgcgacga aaccgccgtt
     7141 gccattttaa atgataatga aattttagtc aatctggtct cttctcaatt acttcatcag
     7201 cagttcggag gcgttgtccc ggaatttgct tctcgtgaac atatgcgtct gcttgatcag
     7261 atggtaaaag aagcattcgt ccagaccgga ttgcagcccg aaaatcttga tggtattgct
     7321 gtgactcgcg gtcccgggtt aatgggagct ttattggttg gattaagttt cgcaaaaggt
     7381 ttggcatatt cacttcgtaa acccttactg ggtgtcaacc acatcgaagg tcatattatg
     7441 gctaattttt tgacctgccc ggatttaaaa tttccctttc tctgtttact ggtttccggc
     7501 ggccataccc aaattatcca ggtgaatgac tttggtaatt atcagattct tgggcacagt
     7561 gttgatgatg ccgccggtga ggcttttgac aaagccgccc gcattctgga attaccttat
     7621 ccgggaggtc cacaaattga tcaaatggca cgtcgtggta atcccaattt ttaccaattt
     7681 ccgcgggcta aagtccgctc cgagcagtac gatttcagtt tcagtggact gaagaccgcg
     7741 atgttatatc tagttcgtca acgaggccaa aaatgggctc aggaaaattt agccgattta
     7801 tgcgccgctt atcaggaagc gatcgttgat gtcctgaaaa tcaacacttt cactgcagtc
     7861 aaaaatctgc acgccaataa actggtctta gccggtggcg ttgccgctaa ttccagacta
     7921 agacaagttt tccaggaaca ggctgattac taccaaatcg aattgataat tccgtctttg
     7981 aaatattgta cggacaatgc cgcgatgatc gccagagccg gacaggaaat gttgtcacgt
     8041 aaccaaattt cgcctttttc aatctcagct gtaccaaatc taacacttac ta
//
LOCUS       DAOOMU010000097         7898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_10795_length_7898_cov_9.243912, whole genome shotgun sequence.
ACCESSION   DAOOMU010000097 DAOOMU010000000
VERSION     DAOOMU010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7898
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10795_length_7898_cov_9.243912"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLI55_08585"
     CDS             complement(<1..241)
                     /locus_tag="PLI55_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00925.1"
                     /translation="MKKWSKILLVIGFLSAESLFAQVIWEKNQYKVHLQNNVLSLFYS
                     QDKIVEISSFSFNFIPVDTIIVEQVFEDSLILKLRL"
     gene            complement(261..1706)
                     /locus_tag="PLI55_08590"
     CDS             complement(261..1706)
                     /locus_tag="PLI55_08590"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HPB00926.1"
                     /translation="MRSNLTVLAVDLGAASGRVIAVNYDGNNFELKELHRFLNIPIVK
                     NGTLYWDISRLWQETKAGLEKGKSLKPASMGVDAWGVDFALLDKQGVLLDNPVHYRDR
                     RTDGMQEKVIARISKQEIYSQTGIQILPINTLFQLSYLVEKQSPVLLNAETFLTIPDL
                     FNYWLVGVKACEFTNATTTQLYNPLQKNWAESLINKLQIPYHIFPDVISPGIQLGKYD
                     GIDVMTVAAHDTASAVAAVPTEIKNFTYISSGTWSLVGQEIATPLINESALKANITNE
                     GGVFGTFLFLKNVMGLWLIQECQRQWRKEGENYTSSDLVKLAKQEPPLRSLIIPDDPQ
                     FLHQGNFPQMIANFCQITSQPMPTTPGAIVRCVLDSLALDYKNVLEKLVGISNRPVEL
                     IHIVGGGSQNDLLNQLTANATGLSVFAGSAEATVLGNAIVQLITLGEIKNLSEARQIV
                     AKMKGIKRYEPKDSALWDEAYQRFLTLQKQF"
     gene            complement(1736..2941)
                     /gene="rhaI"
                     /locus_tag="PLI55_08595"
     CDS             complement(1736..2941)
                     /gene="rhaI"
                     /locus_tag="PLI55_08595"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HPB00927.1"
                     /translation="MNPSGKSSNIFDNPHIDFEKVIHALKSQRIETPSWGYGNSGTRF
                     NTFACAGAARNVYEKIADAAYVHKLTGIAPAVALHIPWDKTDDYTALKQYAESKGIKV
                     GAINPNLFQDKQYKLGSLCNPSKAVRQKSIDHILACCEIMEKTGSNVLSLWLADGTNY
                     AGQDDLFRRKENLESGLKEIYSNLPQNCRMLIEYKFFEPSFYHTDLADWGMAYVMAMK
                     CGPRADVLVDTGHHALGTNIPHIVAFLLSEGKLGGFHFNARKYADDDLIVGTVNPFEI
                     FDVFTKLVSAGSKADKIVYMIDQSHTIETKIEAMIMSIINCQTALAKALIVDYPALQE
                     AQMNGDILVAQSILTKAFETDVYPLLEEVREEMGVPRDPLLAYRKDNYRNKIISERGI
                     SEANNGYPI"
     gene            complement(3002..3178)
                     /locus_tag="PLI55_08600"
     CDS             complement(3002..3178)
                     /locus_tag="PLI55_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015265444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00928.1"
                     /translation="MKKILIIISFIGLALTLLPSFLVFANAISWDTYLLLMNVGTIMW
                     FVSAPFWMEKKDEA"
     gene            complement(3175..4587)
                     /locus_tag="PLI55_08605"
     CDS             complement(3175..4587)
                     /locus_tag="PLI55_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013928217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00929.1"
                     /translation="MKSKRGNVLVKKLDEINEFERTPVPPSKLKGAGSFWGMYAGEHT
                     AGTEFVIGPLFVAHGVSAFDLVTGLLIGNLLAVLSWTFITGPIATRARITLYYFLEKI
                     AGKKVTLLYNLVNALMFCGLAGAMIAVSATAIGIPFNLPMPSLNDFFPNSIGWIVAVI
                     VVGAVTTAVAMFGYSVVAKFGKFAAPWMPLVFIASAVAVLPEIGVSSFSNFWEVAKER
                     IWNGVPLEGQSKFTIWHIIFFSWFCNLAMHIGMADLSILRYAKKWYYGVTSSAGMFVG
                     HYIAWLASGILYSLFLLESNNSLAFSPGQVAYRAAGIAGIACVIIAGWTTANPTIYRA
                     GLALQAVMPKSKTWKVTMIIGAITTFAAFFPALVMRLLDFVSLYGLILMPMGAVIFTD
                     FWLFPKLKLTQNYAEVRKIQFSWPVFLTWCITLLIAFLLPMEIYFKALPGWFIAIILY
                     TGLSYFQQKVLEVRGKKEIL"
     gene            complement(4644..6335)
                     /locus_tag="PLI55_08610"
     CDS             complement(4644..6335)
                     /locus_tag="PLI55_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPB00930.1"
                     /translation="MKKKLSKKIACIAIFLNLVTQLLYSQENVIRIACVGNSITEGNA
                     MATKTLDAYPVALGRYLGAGYEVKNCGVSGTTLLKKGDYPIWNEKLFAEALNFNPNIV
                     TIMLGTNDSKPYNWIYKGEFISDYLAMIDTFRQLSSRPEIYICLPLPAFSGAWDIRDS
                     IIVNDIIPMIRQIADSANVNLIDLHTPFLDKSSLMPDGIHPLIEGSDLIARILMEELT
                     GRRIESQTENDVALCKLAIQSGTNVPELTDGNSNTYAVFSSTSTPVTINLGDEVEVDL
                     VQINFTNISISEFAFTFAASIDSLDWTTLIDTTLLSASLLKCDSTALFSKTFQPIPAR
                     FLKFQIKENGIPDDSGLLINQINIFERRVVHAPIWGWELVSTSDKYMRVKVRTQKTSN
                     IGEYIKIYKQSKADGQFELYANYGSDKPLEMTSFISYGSLNRYYSVVFYNDVEIASDT
                     MTIIARSASDIKNNPKQVLLTNYNLFQNFPNPFNSATNIGFYLAELGEVNIEIIDLNG
                     RLIRSLSPRIFSSGINQIRWDGCNQNGIPSASGIYFYRLKIGDKPSLLKKMVLLR"
     gene            complement(6353..7189)
                     /locus_tag="PLI55_08615"
     CDS             complement(6353..7189)
                     /locus_tag="PLI55_08615"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007808993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB00931.1"
                     /translation="MKCLKFYDIKIGFIILFGIVSIFRENGMAQKNEKNWWNNKKCAV
                     CLTYDDALNVHLDSVIPVLDSLGLKGTFYLSGNFQTVQERANDWKSAADNGHELGNHT
                     LFHPCDGKMPGRSWVNPDYDLSTYTLSRITDEIKMANVLLQIIDGKSKRTFAYPCGDT
                     QVGGVSYINVIKENFVGARGTETRLETIDDIDLYNIGCFMINGESGVELIDLAKTALK
                     NKALIVFLFHGVGGEHNINVSLEAHNQLVNFLKQNEKDIWIAPLAEIAKYIVNYRGKI
                     GK"
     gene            complement(7227..>7898)
                     /locus_tag="PLI55_08620"
     CDS             complement(7227..>7898)
                     /locus_tag="PLI55_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB00932.1"
                     /translation="GGWSPVTTENLTGAVDSLNAYPDKFWVSSFANVARYIQERNNVS
                     ITESVVTDSSITFQVADTLDNEIYNVPLTIRRVLPEGWSAAEIFQNGEKINSQIVEIG
                     TARYLMFDIIPDEGDIILVKSVDNAIQLKNKNTPVIPYLKQNYPNPFNPKTTIAFSIP
                     QKSFVTLKIYNLFGQEVTTLVNESKTPGEYTVEFDGSYLPSGTYFYKIQAGDFSSTKK
                     LILLK"
BASE COUNT         2350 a         1745 c         1489 g         2314 t
ORIGIN      
        1 ccaggcgtaa tttcaaaatt aatgaatcct cgaaaacctg ttcaactata atagtgtcta
       61 ccggaataaa attgaaggaa aaagaactaa tctcgacgat tttatcttgg ctatagaata
      121 aactcaatac attattctgc aaatgaactt tatattggtt tttctcccag attacctgag
      181 caaataatga ttccgccgaa aggaatccaa ttaccaataa aattttagac cattttttca
      241 tcagataatt cctaccaaat ttaaaattgt ttttgcaaag ttagaaatcg ttgataggct
      301 tcgtcccaga gagcgctatc tttaggttca taccgtttta tgcccttcat tttggcaact
      361 atttgtcgtg cttcactcag attttttatt tctccgagag taattagctg gacaatggca
      421 ttccctaata cggtggcttc ggctgagccg gcgaacacgg acaagccggt cgcattggcc
      481 gtcaattgat taagtaaatc attttgtgat ccgcctccga caatatggat aagttccacc
      541 ggacgattcg atattccaac caatttctcg agtacattct tataatctaa tgccagactg
      601 tctaatacac accgtacaat ggctcccgga gtagtcggca tcggttggga ggtaatttgg
      661 caaaaattgg ctatcatctg agggaaattc ccttgatgta aaaattgggg atcatctggt
      721 ataatgagcg accgtaaggg cggttcttgt ttcgccagtt ttactaaatc tgaactggta
      781 taattttccc cctctttccg ccactgtcgt tgacattctt gaattagcca gagtcccata
      841 acatttttta ggaacaaaaa tgtgccgaat acgccacctt cgttggtgat attagccttt
      901 agagccgatt cattgattag cggggtcgcg atttcctgcc caaccaggct ccaggtgccg
      961 ctactgatat aggtaaaatt tttaatttcg gtcggaacag ctgcgaccgc actggcggta
     1021 tcatgagccg ctactgtcat tacatctatg ccgtcatatt tacccagctg aatacctggt
     1081 gaaataacat ccggaaaaat atgatatgga atttgtaatt tattaattag cgactcagcc
     1141 cagttttttt gcagtggatt gtatagctga gtagttgtcg cgttagtaaa ttcacatgct
     1201 tttaccccaa cgagccaata attaaataaa tcgggaatcg tcaggaatgt ttccgcattt
     1261 aataaaaccg gggattgctt ctcgactaaa taacttagct gaaatagggt attgatgggc
     1321 aatatctgaa tgccggtttg ggaataaatt tcctgtttag aaattctggc aatgaccttt
     1381 tcctgcatac cgtctgttct tcggtcgcga taatgaaccg gattatccaa aagaacacct
     1441 tgtttatcga gcaacgcaaa atcaactccc caggcatcaa ctcccatact ggcaggcttt
     1501 aggcttttcc ctttttctag accggctttg gtttcctgcc agagtcgtga aatatcccaa
     1561 tataaagtgc cgttctttac tatcggaatg tttaaaaagc gatgtaattc ctttaattca
     1621 aaattattcc cgtcatagtt tacggcgata acccgtcccg atgcggctcc caggtcaacg
     1681 gctagtacag ttaaatttga tcgcatttaa attttagtga atttagagat tgggtttaaa
     1741 tgggatagcc attattggct tcagagattc ccctttcact tataatttta ttccgataat
     1801 tatccttacg gtaggctaat aaaggatcgc gcggcactcc catttcttcc cgaacctcct
     1861 cgagtaatgg atatacatcc gtctcaaagg ctttggtcaa aatgctctgc gctacgagaa
     1921 tatcgccatt catctgtgct tcttgaagag ccgggtaatc tacaatcaac gccttggcca
     1981 aggcagtttg acaatttatg atactcatta tcatcgcttc tatcttagtt tcgatcgtat
     2041 gactttgatc aatcatatac acgattttat cggctttcga accggctgaa accaatttgg
     2101 taaaaacatc gaaaatttca aacgggttaa cagtacctac aattaaatca tcatctgcat
     2161 atttccgggc attgaaatga aatccgccca gtttaccttc actaaggaga aaagccacga
     2221 tatgaggaat attcgtaccg agggcatgat gtccggtatc aactaacaca tcggctcgcg
     2281 gtccacattt catcgccata acgtacgcca tcccccaatc cgccagatcg gtatgataaa
     2341 aagacggttc gaaaaattta tactctatta acatccggca gttttgcggc aaattggagt
     2401 aaatttcctt caatccggat tcaaggtttt ctttacgccg gaaaagatca tcctgtccgg
     2461 catagttggt accatcagcc agccacaagc tcaaaacgtt gctcccggtt ttttccataa
     2521 tttcacaaca agctaaaata tgatctatgg acttttggcg gactgcttta gagggattgc
     2581 ataaggagcc taatttgtac tgtttatcct gaaacaaatt gggatttatc gcgcctactt
     2641 taatcccttt ggattcggca tattgtttga gagcggtata atcatcggtt ttatcccagg
     2701 gaatatggag tgccacggcg ggagcaatac cggtcaattt atgaacataa gccgcatcgg
     2761 caatcttttc ataaacattc cttgccgccc cagcgcaggc gaatgtatta aaacgcgtcc
     2821 cactattacc gtatccccag ctgggagttt caattcgctg acttttgagt gcgtgtatga
     2881 ctttttcaaa atcaatatgg ggattatcaa aaatattgct cgattttccg gatggattca
     2941 tcactttctc caaataatat tatcaacttt cacttccatg ttttctatgt caatatcaac
     3001 tttaggcctc atccttcttt tccatccaaa agggcgctga tacgaaccac ataatagttc
     3061 caacattcat taataaaaga taagtatccc atgaaatagc gttggcaaat accaggaacg
     3121 acggcaaaag agtcaaagcc agaccaataa acgatataat gattaaaatt ttcttcataa
     3181 gatttccttt ttccctctta cttcgagaac tttctgctga aaatagctta gcccagtata
     3241 taaaatgata gcaataaacc atcccggcaa agccttgaaa taaatttcca tcggcaataa
     3301 aaatgcaatt aataaagtta tacaccaggt taaaaatacc ggccagctga actgaatttt
     3361 cctcacctcg gcataattct gagtcagttt caatttagga aataaccaga aatcggtgaa
     3421 aattactgct cccatcggca tcaaaatcaa cccatagagc gacacaaaat ccagcaatct
     3481 catcaccagc gctggaaaaa aagccgcaaa ggtcgtgatg gcgccgatga tcatcgtcac
     3541 tttccaggtt ttagattttg gcatcactgc ctgaagtgcg agcccagccc gatatatggt
     3601 cggattggcg gtagtccaac cggcaatgat tacgcaagcg atcccggcta ttccggcagc
     3661 acgataagca acctgccccg gtgaaaatgc cagactatta tttgattcca ataaaaaaag
     3721 cgaatataaa attcccgatg ccagccaggc aatgtaatgc ccgacaaaca taccagccga
     3781 agaagtaacg ccataatacc actttttggc ataacgcaaa atggatagat cagccattcc
     3841 gatatgcatc gctagattac aaaaccaaga gaaaaatata atgtgccaaa tggtgaattt
     3901 actttgacct tccaggggaa cgccgttcca gattctttcc ttggcaactt cccagaaatt
     3961 ggaaaaagaa ctaactccta tttccggtaa aaccgctacg gctgaggcaa taaataccaa
     4021 aggcatccaa ggtgccgcaa atttgccgaa cttggcaacc accgaatagc cgaacattgc
     4081 caccgcagtt gtgacggcac caaccacaat caccgccaca atccagccaa tactattggg
     4141 gaaaaagtca tttaaagaag gcattggcag attaaatgga atcccaatcg ccgtcgctga
     4201 tacggctatc attgcgcctg ctaaaccaca aaacattaga gcattgacca gattatacaa
     4261 caaggtcact tttttaccgg cgattttttc taaaaaataa tagagggtaa tacgggctct
     4321 cgtcgctatc ggaccggtaa tgaatgtcca actcaaaacg gctaaaagat ttccaatcag
     4381 caatccagtt actagatcaa aagcgctgac gccatgggcg acaaaaagtg gcccaatgac
     4441 aaattcggtt ccggctgtat gttcaccggc atacattccc cagaaactgc ctgccccttt
     4501 taatttcgag ggaggcacgg gagttcgttc gaactcattg atttcatcta gtttctttac
     4561 taaaacgtta ccccgtttag acttcatgcg atttctccaa actttgatat cgaatatcta
     4621 cttcaatcac caaatgaacg ttcctaccgt aataatacca tttttttgag caggctgggt
     4681 ttatccccga ttttcaaacg ataaaaatat attcctgacg ccgaaggtat gccgttctga
     4741 ttacagccat cccacctgat ctggttgatg ccgctggaaa aaatccgagg gctaagcgac
     4801 cggattaatc tcccattcaa gtcaattatc tcaatattta cttcacctaa ttccgctaaa
     4861 taaaagccga tattagtagc gctgttaaaa ggattaggga aattctggaa aaggttatag
     4921 ttcgtcagaa gaacttgctt cggattattt ttaatgtcag aagcgcttcg ggcaattatc
     4981 gtcattgtat cggaggcaat ttcgacatca ttataaaata cgacactgta atagcgattc
     5041 aaggaaccat aggaaatgaa actcgtcatc tccagtggtt tgtccgagcc ataattggcg
     5101 tataattcaa attgcccatc ggccttcgac tgtttgtaga tttttatata ttcaccgatg
     5161 tttgaggtct tttgcgtacg cacttttacc cgcatatatt tgtcggaagt cgagactaat
     5221 tcccagcccc agatgggagc atgtacaact cgcctctcga aaatatttat ctggttaata
     5281 agtaaaccag agtcgtcggg aattccattt tctttaatct gaaattttag aaatcgagcc
     5341 ggaattggtt gaaatgtctt actgaataat gccgtactat cacattttaa taacgaggca
     5401 ctaagtaaag tagtgtcaat tagcgttgtc caatcgagcg aatcaatcga agccgcaaaa
     5461 gtaaaggcaa attccgaaat tgaaatattg gtaaaattaa tctgaactaa atcaacttca
     5521 acctcatcgc ccaggtttat tgtaaccggc gtgctggtag aagaaaatac agcataagta
     5581 tttgaattcc catcagtaag ttctggtaca tttgttccgg attgaattgc cagtttgcaa
     5641 agagcgacgt cattttcggt ctgggattca attcttcgac cggttaattc ctccataagt
     5701 atgcgggcta tcaaatcaga accttcgatt agcgggtgaa ttccatccgg cataagactg
     5761 cttttatcca gaaatggtgt gtggagatca atcagattaa cattggctga gtcggcgatt
     5821 tgtcttatca tagggataat atcattgacg atgattgaat ctctaatgtc ccaggcaccg
     5881 ctgaaagccg ggagtggcag gcaaatataa atctcaggac ggctggataa ctgccggaaa
     5941 gtatcaatca tcgcgagata atcggaaata aattcacctt tataaatcca gttgtaaggt
     6001 ttgctatcgt tcgtacccag cattatcgtg acaatgttcg gattgaagtt gagcgcctcg
     6061 gcaaataatt tttcgttcca tatcggataa tcgccttttt tcaataatgt cgtgccggaa
     6121 acgccacaat tttttacttc atacccagcg cctaaataac gccctagagc caccggataa
     6181 gcatccagag tcttagttgc catagcgttg ccttccgtaa tgctatttcc gacgcaagca
     6241 attcggatga cattttcttg ggaataaagt aattgagtaa ctaggtttaa aaaaattgct
     6301 atacaagcta ttttttttga cagttttttc ttcattcaaa tctctctatc tatcatttgc
     6361 cgatttttcc tcgataatta acgatatact tggcaatttc tgccagcggc gcgatccaga
     6421 tgtctttctc attttgcttt agaaaattaa ctagttgatt gtgagcctcc agagatacat
     6481 taatattatg ttcgccgccc acaccgtgaa acaagaatac gattaaagcc ttatttttta
     6541 aagcagtctt agctaggtca atcaactcga ctcccgactc tccattgatc ataaaacaac
     6601 cgatattgta taaatcaatg tcatcaattg tttcgagccg ggtttcagta ccacgggcac
     6661 cgacgaagtt ttctttaatt acattaatgt acgaaacacc accgacctgc gtatcgccac
     6721 atgggtaagc aaatgtcctt tttgatttcc catcaataat ttgcaacaga acattagcca
     6781 tttttatttc atccgttata cgcgaaagcg tgtaagtgct gaggtcataa tctggattga
     6841 cccaactccg acctggcatt ttcccgtcac aaggatgaaa taaagtatga ttaccgagtt
     6901 catgtccgtt atcagcggct gatttccaat cgtttgcccg ctcttgaact gtttggaaat
     6961 ttcccgacaa ataaaaagtg cctttaagtc ccagagaatc caacaccggt atcaccgaat
     7021 ccaaatgcac gtttagagca tcgtcataag tcaggcaaac cgcacatttt ttattattcc
     7081 accaattctt ctcatttttc tgcgccattc cgttctccct gaaaattgaa acgattccga
     7141 acaaaatgat aaatcctatt tttatatcgt aaaattttaa gcatttcata aatcactccc
     7201 tataattatt cgaactgatc atttgactat ttcaataaaa tcaatttttt agtactggaa
     7261 aaatctccag cctgaatttt atagaaatag gtaccgctag gaagataact gccgtcaaat
     7321 tcaaccgtat attcacccgg agttttactc tcatttacca aggtggttac ctcctgcccg
     7381 aataagttat atattttcaa ggtcacgaaa gacttttgag gaattgaaaa agcaatggta
     7441 gttttcggat taaagggatt agggtaattc tgctttaaat aaggaataac cggcgtattt
     7501 ttatttttta attgaatggc attgtctacg cttttcacta agataatatc gccttcgtcc
     7561 ggaataatgt cgaacattag atacctggct gtaccgatct caacaatttg ggaattgatc
     7621 ttttcgccat tttgaaaaat ttcagccgct gaccaacctt ccggtaagac gcggcgaata
     7681 gttaacggaa cattataaat ttcattatcc agagtatcgg caacttggaa agtaatacta
     7741 ctatcggtaa caaccgattc ggtaattgat acattattgc gttcttggat atagcgcgcc
     7801 acgttggcaa aggaggatac ccagaattta tccggatagg catttagaga atcaaccgca
     7861 ccagtcagat tttccgttgt cactggcgac caaccgcc
//
LOCUS       DAOOMU010000098         7655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_11285_length_7655_cov_23.128684, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000098 DAOOMU010000000
VERSION     DAOOMU010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7655
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11285_length_7655_cov_23.128684"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1149
                     /gene="carA"
                     /locus_tag="PLI55_08625"
     CDS             97..1149
                     /gene="carA"
                     /locus_tag="PLI55_08625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPB00933.1"
                     /translation="MNKRTALLTLEDGHVFEGFSFGWEGATAGELVFNTGMVGYPESL
                     TDPSYYGQILVMTYPLIGNYGVPEFKPDSLYFESDRIQVRGLIVADYSADYCHWNAHQ
                     SLGDWLKSQKIPAITGIDTRTLTQILREEGTMLAKITFDDDIDYYDPNRYNVVAEVTC
                     SEPRTYGQGKKRIALLDCGCKRSIISQLSQHKVEIMRLPWDSDLTKYDFNGLCISNGP
                     GDPRNCEASVASVRLALENKIPTLGICLGNQILALAIGAKTYKLKYGHRGQNQPVMDQ
                     TSGCCYITSQNHGFAVDDKSLPEDWRIWFSNLNDRTNEGLIHRSGRYMSVQFHPEAAT
                     GPLDTMFVFDRFLDIL"
     gene            1298..4477
                     /gene="carB"
                     /locus_tag="PLI55_08630"
     CDS             1298..4477
                     /gene="carB"
                     /locus_tag="PLI55_08630"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HPB00934.1"
                     /translation="MVKKVLILGSAALKIGEAGEFDYSGSQAIKALKEEGIHTILVNP
                     NIATIQTSENLADEIYFLPVNAEFVEKVIKKTRPDGILLSFGGQTALNTGLDLHRRGI
                     LQKYDVAILGTPIQAIKDTEDRQLFIQRLQEIEVKTARSQIATTLDEALQAAEALAFP
                     VIIRIAYALGGLGSGVCRNLEEFRDKAEKALTYAPQILIEEYLEGWKEIEYEVICDSF
                     NNCLTICNMENVDPMGIHTGESIVVAPCQTLSNSESHKLRSIAIKVVRHLGIVGECNI
                     QFALDPRSEDYRVIEVNARLSRSSALASKATGYPIAFIAAKLALGYGLHQLPNKVTRT
                     TTAFFEPALDYIVVKIPRWDLKKFRNVSHRIGSGMKSVGEVMAIGRTFEEALQKGLRM
                     LETGANGIVGNDGVIFENLERELSEPTDERIFAVAEALQQGYDVAKIAKLTNINPWFI
                     YKIRNIVAMNTHLSQYRNQELPTDTLLAAKKLGFSDKQIAFAIGKSEAEVHQLRLKAN
                     IRPYIRQIDTLAAEFPAYTNYLYLTYNAIRDDISFTDSNVVMILGSGAYRIGSSVEFD
                     WSCVNCGQTLRRLGYNTIMLNYNPETVSTDYDESDFLIFDEISFENVVEIYAKFSALG
                     VIVSMGGQIPNNLALKLHHANVRILGTSPESIDRAENRRKFSDLLDKLNVDQPIWKEI
                     TSVELAKEFAHEHGYPVLIRPSYVLSGAAMAVASNDEELVQYLQRAVKISPEYPTVIS
                     KFLENAKEIECDAVARNGEIITYAISEHVENAGVHSGDATLVLPPQRTYLETIRRIRI
                     ITSEIAQNLKINGPFNIQFIAKQNEVKVIECNLRASRSFPFVSKVLKRNFIDIATQVI
                     MHVPLEPIPVSLFELNHVGVKAPQFSFTRLQGADPTLGVEMASTGEVACLGDDFDEAF
                     LKALISVGYHFPPRAALISSGPIESKAELLESIRTLQNMNVKLYATGGTAKFLEANKV
                     SVETLFWPLEKKEPNVITYLKNGKIDLVINIPKSYQERELTNGYLIRRTAADFNVMLI
                     TNRQIAMRFIEAISRLKMDDLKIRSWGEY"
     gene            4490..5317
                     /gene="mutM"
                     /locus_tag="PLI55_08635"
     CDS             4490..5317
                     /gene="mutM"
                     /locus_tag="PLI55_08635"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HPB00935.1"
                     /translation="MPELPEVQTIINDLIAANLVGLKILEVEIRWQKIIAEPSAAKFT
                     TEIQNREITKITRRGKFIVIELKPVKFLLVHLRMSGKFSLTVPDYPISKHEHLIFTLS
                     DGRQLRYYDTRKFGRFWLVNQPEKVIGKLGVEPLSADFTVEILTENLRKHHRQIKPFL
                     LDQTIIAGLGNIYVDEALWEACLHPLRMADSLNDQEIASLHKAIPKVLRQGLANQGTS
                     LGNGKGNFQSVGKRARNQEALKIFRRTGQACPRCGAIIERLKVGQRSTHICPHCQLI"
     gene            5449..6339
                     /locus_tag="PLI55_08640"
     CDS             5449..6339
                     /locus_tag="PLI55_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPB00936.1"
                     /translation="MYKVYLRIFQVSTALLVIALAISTFLSPEAGWGVPLTQPISQIV
                     RALSLDHFPNSWLNVVLWLVLITGMLAAVFLKGIKGFAQKWLHFWLAAVFLLIFYDKA
                     FNQRFVISIQEGQEVNFAQFLKKPKPEYNIPLRLLDFDIKIHPDGKTAASYASRLVVN
                     QTDTAILAVNHPLSIGKYRLYQSAFRKDYLFEVICDGDTIFSPFNGACNFNQQEIFLR
                     GVDTLAQALTVEIDRNEYKIPPGEVCNVAGRQIKINPLSPKYTSIIEVAEVTGLKFLA
                     FFSLMYLGVLTYIILWKKIR"
     assembly_gap    6476..6575
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6576..6999
                     /gene="ccsA"
                     /locus_tag="PLI55_08645"
     CDS             <6576..6999
                     /gene="ccsA"
                     /locus_tag="PLI55_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPB00937.1"
                     /translation="PALNSFWMYLHVPAYFFGYMALVLAVIYALVFLVQNKNESSSDG
                     MTLLRKMDNEVQIAFLFLNIGLITGAFWGYQSWGNYWSWDVKEVWALLNILVLAFYFH
                     LLKPRGVKKAVIVIIAFLTVIFTYFGVSFLLKGLHSYL"
     gene            7134..7580
                     /locus_tag="PLI55_08650"
     CDS             7134..7580
                     /locus_tag="PLI55_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPB00938.1"
                     /translation="MERSQFENILDFAIEREWESVRFYHRLLQEAPSLAMMEAIRGVE
                     LVERQHINTLEQFRKAGFKSQAIPSVDNIQLGEYYVPAVENQNYRNMLMTAIQREELS
                     HKLYKALAETSRDPEVADLFEKLASEETQHKSYFESLYDSEVLKEN"
BASE COUNT         2159 a         1584 c         1677 g         2135 t
ORIGIN      
        1 atttactgaa attcttgaat tgattgatta aattacgccc cgattgaaga tctatgaaat
       61 taactgaata aaattcttgg aaagtcaagt aaatcaatga ataaacgcac ggcgctttta
      121 accctcgagg acggtcatgt tttcgagggt ttttcttttg gctgggaagg tgcgaccgcc
      181 ggtgaactgg tctttaatac cggtatggtc gggtacccag agagtctgac tgatccttcc
      241 tattacggac agattttagt gatgacttac ccattaatcg gtaattacgg tgtgccagag
      301 ttcaagcccg atagcctgta ttttgaatcc gatcggattc aggttcgtgg gctgatagta
      361 gctgattatt cagcggatta ttgtcattgg aatgcccacc agtcgctggg cgattggcta
      421 aaatcacaaa aaattccagc cataaccggc attgataccc gcactttaac tcagatttta
      481 cgggaagaag gcacaatgct cgccaaaatt acttttgacg acgacattga ttactatgat
      541 cctaaccgtt acaacgtggt agcggaagta acctgctccg aaccgcggac gtatggccag
      601 ggcaagaaac gaatcgcact tttggattgt ggatgtaagc ggagtataat ctcgcaattg
      661 tctcagcaca aagttgaaat tatgcgtttg ccttgggaca gcgatttgac caagtatgat
      721 tttaacgggt tgtgtatttc taatggccca ggtgatcctc gaaattgtga agcttctgtt
      781 gcctcagttc gactggcatt agagaataag ataccgactc tgggcatttg cctcggtaat
      841 caaattctag ctttggcgat aggggcaaag acttataaac ttaaatatgg ccatcgggga
      901 caaaatcaac cggtaatgga tcaaaccagc ggatgctgtt acatcacctc gcagaatcat
      961 ggctttgcgg ttgatgataa gtccttgcct gaggattggc gaatctggtt tagcaatttg
     1021 aatgatcgaa ctaatgaagg cttaatccac cgctccgggc ggtatatgag cgttcagttt
     1081 catcctgaag ccgcgactgg cccgcttgat acgatgtttg tattcgaccg attcctggat
     1141 attttataaa aggagagaac gcgaaaaacg attcagtgtt aattaatgga tattataatg
     1201 ccaattataa aaattctcaa aacaaataac cgggaaaaaa gtaggattga attatcagtc
     1261 ttggcgatct cagcgccctc agcattgaaa ggaaaaaatg gttaaaaaag ttctcatcct
     1321 cggcagtgcc gcccttaaaa tcggagaagc cggggaattt gattattctg gcagtcaagc
     1381 catcaaagcc ttaaaagaag aaggcattca taccattctg gtaaatccca atattgctac
     1441 gattcagacc tcggaaaatt tagccgatga aatctacttc ctaccggtta acgccgaatt
     1501 tgtcgaaaaa gtaattaaaa aaaccagacc tgatggtatt ttgcttagct ttggcggaca
     1561 gaccgccctt aatactggtc tggacttgca ccgtcgcggt attctccaaa aatatgatgt
     1621 tgcaattctg gggactccta ttcaagccat taaagatact gaagaccgcc aactttttat
     1681 ccaacgtctg caggaaattg aggtcaaaac tgcacgtagt cagatagcca ctactctgga
     1741 tgaagcttta caagccgctg aagctctagc tttcccggtt attattcgga tagcgtatgc
     1801 tctcggcgga ctgggttcag gcgtctgccg taatctggaa gagttccgtg ataaagccga
     1861 aaaagcactg acttatgccc cccagatttt gatcgaggaa tatctggaag gctggaaaga
     1921 gatcgagtac gaagtaattt gcgatagttt taacaactgc ctgacgatct gcaatatgga
     1981 aaatgtggat ccgatgggca ttcataccgg cgaaagcatc gtggtagcgc cctgccaaac
     2041 actttccaat agcgaaagcc ataagttgcg ttctatagcg ataaaagtcg ttcggcacct
     2101 cgggatagtc ggtgaatgta acatccagtt tgcactcgat ccgcggtcag aggattatcg
     2161 cgtcatcgag gtgaatgccc gattgtcgcg cagttcggcg ctggcttcta aagcaaccgg
     2221 ctatccgatt gccttcattg ccgcaaaact agcactcggc tacggattgc atcaattgcc
     2281 taataaagta acacgtacaa cgactgcctt cttcgaaccg gctctggatt atatcgtggt
     2341 taaaattccc cgctgggatt tgaaaaaatt ccgcaacgtc agtcatcgca tcggttcagg
     2401 gatgaaatca gtcggcgaag taatggcgat tggacgaaca ttcgaggaag ctctccaaaa
     2461 aggcttgcgt atgctcgaaa ccggtgccaa tggtattgtc ggcaatgacg gtgtaatatt
     2521 tgaaaatctg gaacgagaac tgagcgaacc gaccgatgaa cgtatctttg cggtcgcgga
     2581 agctctccag caaggctatg acgtcgctaa aatcgccaag ttaaccaaca taaatccctg
     2641 gtttatttac aaaatccgca atatcgtcgc aatgaataca cacttgagcc agtatcgaaa
     2701 tcaagaatta ccgaccgata ccttactagc ggctaaaaaa ctcggctttt ccgacaagca
     2761 aatcgctttc gctatcggga aatcagaagc cgaggtacac caattgcgtc tgaaagccaa
     2821 tattcgccct tatattcgac agattgacac gctagcggct gaattcccgg cttataccaa
     2881 ttacctttac ctaacctata atgccattcg cgatgatatt agttttaccg attccaatgt
     2941 ggtcatgatt cttggttcag gggcttaccg tatcggtagt tcggtcgaat ttgattggag
     3001 ttgcgtgaat tgcggccaaa ctctgcggag gctcggatat aataccatta tgctgaatta
     3061 caatccagaa acggtcagca ccgattatga cgaatccgat ttcctgattt ttgatgaaat
     3121 tagttttgaa aatgtagtgg aaatttatgc caaattcagc gccctcggag taatcgtttc
     3181 gatgggtggt cagattccca ataacttggc tttaaaactg caccacgcca atgtccgaat
     3241 tttgggcaca tcgccggaaa gcattgatcg ggctgaaaat cggcgcaaat tctcggattt
     3301 gctcgataaa ctcaacgttg atcaaccaat ttggaaggaa attacttctg tagaattggc
     3361 taaggaattt gcccacgaac acggctatcc ggttttaatt cggccttcgt atgtcttgag
     3421 cggagccgct atggcggtgg cttctaatga cgaggaactt gtccaatatt tacaacgcgc
     3481 cgtaaaaatt tcgccggaat atccgaccgt cattagcaaa tttctggaaa atgctaagga
     3541 aattgaatgt gacgccgtcg cccgtaatgg cgaaattatc acctacgcta tttcggaaca
     3601 tgtcgagaac gccggtgttc attccggcga tgccaccttg gtcctgccac cgcaacgcac
     3661 ttatctggaa actatccgac gcattcggat tattaccagc gagattgctc agaatttaaa
     3721 aattaatggt ccgttcaata ttcagttcat tgccaaacag aacgaggtaa aagtaatcga
     3781 atgtaattta cgggcatcgc gcagttttcc gtttgtttca aaagtcttga agcgcaattt
     3841 tattgacatc gctacccagg ttattatgca cgtgccactc gaaccaatac cagtatcact
     3901 gttcgaactc aatcatgtcg gcgtaaaggc gccccagttt tcctttacac gtttgcaagg
     3961 tgctgatccg actctcggcg tagaaatggc ttctaccggc gaagtcgctt gcctcggtga
     4021 cgatttcgat gaagcttttc tgaaagcgct gatttcggtc ggctaccatt ttccgccgcg
     4081 cgcggcttta atttcatccg gcccaatcga atctaaagcc gaactactgg aatcaatccg
     4141 aaccctccaa aatatgaacg tgaagctgta cgccaccggt ggaacggcaa aattcctcga
     4201 agcaaataaa gtttcggttg aaaccttgtt ctggccgctc gagaaaaaag aaccgaacgt
     4261 cattacctat ctcaaaaatg gcaaaattga tttggtgatc aacattccga aaagctacca
     4321 ggaacgcgaa ttgactaatg gctatttaat tcggcgaaca gcggctgatt ttaatgtaat
     4381 gttaattacc aatcgccaga tcgcaatgcg cttcatcgag gctatctctc gcctgaaaat
     4441 ggatgatttg aaaatcagaa gttggggcga atattgagga aaccgctccg tgcccgaatt
     4501 accggaagtc caaactatca tcaatgatct tatagccgca aatttagtcg gtctgaaaat
     4561 tcttgaggtt gagattcgct ggcaaaaaat catcgccgaa cccagtgccg caaagttcac
     4621 aaccgagatt caaaaccgag aaataactaa aattacccgg cgcggaaaat ttattgtaat
     4681 tgaactaaag ccggtcaaat ttctcctcgt tcatctgcgt atgtcaggga aattctccct
     4741 gacagtgccc gattatccaa ttagcaaaca tgaacattta attttcacgc taagtgatgg
     4801 tcgccaacta cgttattatg acacccgcaa gttcgggcgt ttctggttag ttaatcagcc
     4861 ggaaaaagta attggtaaac tgggcgttga gccgctaagt gccgatttca cggttgaaat
     4921 tctaactgaa aatttgcgta aacatcaccg tcagattaaa ccgtttttat tggatcagac
     4981 aattatcgcc ggtttgggca atatttacgt tgacgaagcg ctctgggaag cctgccttca
     5041 tcctttaaga atggctgatt ctctcaatga ccaggaaatt gcatctttgc ataaagctat
     5101 tccaaaagtt ctacgacagg gtttagccaa tcaaggaaca agcctcggca acggcaaagg
     5161 caattttcaa tcggttggca agcgcgctcg taatcaagaa gcgctgaaaa tctttcgccg
     5221 taccggtcag gcttgcccgc gttgcggcgc gattattgaa cgtctcaaag ttggccagag
     5281 aagtacacac atctgtccgc attgccagct aatctgacct atctcaatta ttttatctga
     5341 atgtccgtct ttttgattcc ctgaaaattt cctttaattt taatacgtga ataaagatag
     5401 ctttaaacga tttcccaatt tggcagattt tattatacag taattgtctt gtataaagtt
     5461 tacctaagaa tatttcaggt ttcgacggcg ttgctggtca tagctttggc cataagtact
     5521 tttctgtctc ccgaagccgg atggggcgtt cctctgactc aaccaatatc gcaaattgtg
     5581 cgagcgctta gccttgacca ctttccaaat tcctggttaa atgtcgttct ctggctggtg
     5641 ttgataactg gaatgttagc ggctgtattt ctaaaaggca taaagggttt cgctcaaaaa
     5701 tggctgcatt tttggctggc ggctgttttt ctcctaatct tttatgataa agctttcaac
     5761 caacgttttg ttatctcgat tcaggagggg caggaagtca attttgccca atttttgaaa
     5821 aaaccaaaac cagaatacaa tattccattg cgacttctcg atttcgatat aaaaatccat
     5881 cctgatggaa aaacggcggc gtcttacgcc agccgactgg tggttaatca aaccgataca
     5941 gccattctgg ctgttaacca tccgctcagt atcggtaaat atcggttata tcaaagtgct
     6001 tttagaaagg attatttatt tgaagtaatt tgcgacggag ataccatatt ttcgcctttt
     6061 aatggggctt gcaactttaa tcagcaagaa attttcctga gaggtgttga tactttagca
     6121 caggcgttga ctgtcgaaat tgatcgaaac gaatataaaa ttccacccgg tgaggtatgc
     6181 aatgtcgccg gtcgccaaat taaaattaat ccgctaagtc caaagtacac ctcaatcatt
     6241 gaagtcgccg aagtaacagg cttaaagttc ctcgcatttt ttagtctaat gtatttaggt
     6301 gtgctgacct atatcatttt atggaagaaa attcgttaaa aaaggggaaa ccgcagtgga
     6361 acaaccgctt ttaatcggcg ccttgatttt atacacaatt gcttttgtta gtctgattac
     6421 aaaagtcaaa gaccaaattt gttccgttgt gatctggtta gccattttaa cccatnnnnn
     6481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcctg ccttgaatag tttctggatg
     6601 tatctgcacg tgccggcata tttttttggt tatatggcgc tggttctagc agttatttat
     6661 gctttggttt ttctggtgca aaataaaaat gaaagttcaa gcgatggaat gacgttactg
     6721 agaaaaatgg ataatgaagt gcaaattgct tttctctttc tgaatatcgg attaataact
     6781 ggagcattct gggggtatca gagctggggc aattattggt cgtgggatgt caaagaagtc
     6841 tgggcattgc ttaatattct ggtcttagcg ttttattttc acttgctaaa gccgagaggg
     6901 gtcaagaaag cagtaatcgt cataattgct tttctgacag tgatttttac ttatttcgga
     6961 gtgagttttt tattaaaagg actgcatagt tatctttgat aaatactatt tttctattca
     7021 agacgatata gtaatgtggt ttgaaatccg ggagcggcag aaatcttgcg ttggagaata
     7081 aattaggcta aattgcatat agtttattga gttaataaat cgcaggcgaa acgatggagc
     7141 gcagtcaatt tgaaaatata ctggattttg caattgagcg cgaatgggaa tctgttcgtt
     7201 tctatcaccg tttgttgcag gaagcgccat cactggctat gatggaagct attcgaggag
     7261 tcgagctggt ggagcgtcag catatcaata cattagaaca atttcgtaaa gccggtttca
     7321 aatcgcaggc aattccatcg gttgataata ttcaacttgg tgaatattat gtacctgcag
     7381 tcgaaaatca aaattatcgc aacatgctta tgaccgctat tcagcgcgaa gaactttctc
     7441 ataaattata taaagcttta gccgaaacca gtcgtgatcc cgaggtagcg gatttgttcg
     7501 aaaaattagc ttcggaagag acacagcata aaagttattt tgagagccta tacgattccg
     7561 aagtgctcaa ggaaaattaa accgggtgaa ttggagagaa tcaagatttg tcttgatttt
     7621 aacttacgga aattttcagt gaaaattcat taaac
//
LOCUS       DAOOMU010000099         7366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_11930_length_7366_cov_17.815484, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000099 DAOOMU010000000
VERSION     DAOOMU010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11930_length_7366_cov_17.815484"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLI55_08655"
     CDS             complement(<1..395)
                     /locus_tag="PLI55_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00939.1"
                     /translation="MKIRRVLLCIVLLVSGTVLAGGLVTNTNQSAAWVRTLNRNASTD
                     VDAVYYNPAGLTKLFEDGLFLDVSNQTIWQTKTVTNDTLTLNNHEFVGKVFAPVFPSA
                     YVAYKMNKLVLSGGFMPIGGGGSAKFEKG"
     gene            complement(571..1890)
                     /locus_tag="PLI55_08660"
     CDS             complement(571..1890)
                     /locus_tag="PLI55_08660"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPB00940.1"
                     /translation="MKIIWDKRRSFFGELILLLLVFSEINGQTAKLRHSTVITLPVDE
                     ETQDFRIGLDLLIDDNFHALDNYKFMVIADRNSFAQGNKYIFDIVNIRLGTRCRSCVQ
                     VLDDRSLLTGSGFQLSQSQLYGITKYYILNSERDRLSASMLNGAEMIVLDLQDFGLRE
                     SEALRILIEALKFSAKQKIPIIVLDRPNPLRGVEIGGPVSIQSKFAADFSLPARHGMT
                     LGELALLMNEENWLNAGDKSQLTVIKMANYYRDMPWQQTGLKWSFADESVQDEETAVL
                     YAGLYFFQFTNISVGTGTPHPFHVVGAPWMSANELVNALAKYDLAGLEISQVKFIPRR
                     ELNATELPLYMDTECLGINLKVSDLNKFDAYKFSATLISVISRLYPKHFKWIKTTEAD
                     AYFGNADFRTWVNIGADLNPMLADWEAKASRFQKLRLKYCLYPVQKN"
     gene            complement(1898..3571)
                     /locus_tag="PLI55_08665"
     CDS             complement(1898..3571)
                     /locus_tag="PLI55_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB00941.1"
                     /translation="MASEPATVKNRSPWLWVPSLYFAEGIPYVIAMTVSVIMYKTMGV
                     SNAALAFWTSVLYLPWVIKPIWSPFVDVISTKRNWVLITQFILGCCFIGVALVVRLPL
                     WFALTLIVLWIIALASATHDIAADGFYMIGLDAHQQTWFVGIRSTFYRMAMIVGQGLI
                     VMLTGYIMSKTGLKPVSITAMAVPPNEIIQVLDENNLPAIKASEQPNIVVIPAEVKVP
                     IYNPAEPGSDSANVFICLSAPPKKEETVVVNFGRKKGSKDIWLTSASRLEFTNENWNK
                     PVKVTLKVDHRLREPVVSQFEATAGDIPFSWAVSFGILGVFFLLICLYHKVMLPTGLE
                     TVRTKSQELKASWFMVFSSFFRKKGIVSALLFLLFYRLAESQLVKMASPFLLDSQEAG
                     GLALTTTQVGFVYGTVGIICLTIGGILGGIVAARNGLKRWVLWMALAINIPDLVYVYL
                     SYVQPDNFMIVTICVAIEQFGYGFGFTGYMLFMLYLAEGEYKTSHYAIATAFMALGMM
                     LPGMISGKLQDLMGYRTFFVYIMFCTIPSFLVASLVKIDPDFGKKKIEN"
     gene            complement(3591..4940)
                     /locus_tag="PLI55_08670"
     CDS             complement(3591..4940)
                     /locus_tag="PLI55_08670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPB00942.1"
                     /translation="MLEPNVIPTEEPILRVGIFLPIDKCQRIALVIPQSPVYELIPES
                     GPRQILKGGEQIEFILFENQIGIQINELTPNAQLSWKIIPRGGEIQIANKAGIKVKNV
                     VAGRGFHWEKYIDVYLPGSIEISRMNDNLMMINVLPLEQYLMCVATSEMGAACPLSVI
                     KSQTIIARSWLLANVEMKHRNLGMDVCNDDCCQRYQGTTFLSEHSIKGALETTGQVLI
                     YGEKICDTRYHKNCGGVTESFENVWNGSAVPYLDSVIDIPPDCPHSALPLDTEAKVRN
                     WIENPPLAFCCPSIVPEKELKKYLGNVDKEGHYFRWHFRMKQREITALFNLKLNLDAA
                     AIKSIEPLKRGKSGRLTSLAINYRDKANVDKQIVINDQYAIREAFHELFLYSSAFVVD
                     VEEGSENIPAAFVLKGAGWGHGVGYCQIGALGMALKGYSTLEILEHYYPGVKLHQAY"
     gene            complement(4922..6169)
                     /locus_tag="PLI55_08675"
     CDS             complement(4922..6169)
                     /locus_tag="PLI55_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00943.1"
                     /translation="MANCKKDIADDEFLLIGIDGGATKVSGWTVKAENHGTAFALGNF
                     HATKSYTEIPGYIHNFQPVDLNRQIAEWEAGKIVPTTAELVQSNCFIKAASWCIEQIT
                     KRSGTNRVLIGIGMPGIKTRDCRGIAVLKNGPRITNYAVRLENEITFAGIELVQPINQ
                     IGSDAYYCGIGEEYAGEGSFRNVRNSYYLGGGTGAADALKLDGKVIPLDETKGWFVKT
                     WEMKCPDGFSVEKYVSSSGIQSIYGELIGKSPEELTRNGIFPPQIRRRALDGEKAAVI
                     TMQKVAHFLALLFYERITSIYAGWQGIFDFVNPDRPRPQTENNYKKALFDNLVIGQRL
                     GELLCEAQGDEVLWLPFVNELTQLIINSSVLDEVAKEHYCPQKHLNLARIQISPLREA
                     PALGAAIDAYLTWKEKKLCLNRM"
     gene            complement(6172..>7366)
                     /locus_tag="PLI55_08680"
     CDS             complement(6172..>7366)
                     /locus_tag="PLI55_08680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPB00944.1"
                     /translation="IQEIRLLKHHPGRLRDNQDYGKVFLFTKGALEAVGGFDQSLKYN
                     ALYDIRLKLSEKYKLVHIANKIDGSLYRVVSGGVKHNVFDYLLVSKEAQLEAEVVLTN
                     HLKRIGAYLAPDQDYCLRPDLNEKYDYIASVIIPVYHRPEFIGNALNSVFSQSIKDLE
                     IIVVVNGGILDPTVTEVRRYMAGGDRYDAQQPPVRLIVADINNIGLSLNLGLKIARGR
                     YYVQLDSDDRLKSDAIEKIVAVFNSDERIGMVIGSYEVWEKKPSGEFVRMEEIPIVKH
                     EEWTDANGRNNLLRINGAGAPRAIPIKIIREMGYFGMNDEPFACNYGEDYDLVLKISE
                     QYRIGRVWQPIYEVVRHTGGTDHSIDQNTIDRNDEAKDYMRLKAVHRRKKINEITNRN
                     LEKSL"
BASE COUNT         2110 a         1801 c         1397 g         2058 t
ORIGIN      
        1 aaacccttct cgaatttggc actgccgccg cccccgatcg gcataaagcc tccggaaagt
       61 acaagtttat tcattttata agcaacgtaa gccgagggaa aaaccggcgc aaatactttg
      121 ccgacgaact catgattgtt caacgtcagg gtgtcattag tcaccgtttt ggtttgccag
      181 atcgtctgat tgctgacatc cagaaacaaa ccgtcctcga ataatttagt caatccagcc
      241 ggattgtagt aaaccgcatc cacatcggtg gaagcgttac ggttcaaagt ccgcacccaa
      301 gccgcgcttt ggttagtgtt cgtaaccaaa ccaccagcta aaacagttcc gctcaccagt
      361 agaactatac ataacaaaac acgtctgatt ttcatgtttt tctcctttat ttttactcgt
      421 tattagagtt atgatgaaga atttttgttt tgattttttc cccttcggct accctacagt
      481 cctgctttac cggtatttaa aataaccact caatctataa ttaaccacta acctcagtat
      541 aaatatatcg aaaagaaaag gaaaacaaaa ttaatttttt tgaaccggat acaggcaata
      601 tttcaatcgt aatttttgaa atcggctggc tttcgcctcc cagtcagcta acatcggatt
      661 caagtcagcg ccaatattga cccaggttcg aaaatcagca ttgccgaaat aagcatcggc
      721 ctctgtggtt tttatccatt taaaatgttt tgggtaaagc cttgaaatca ctgaaattaa
      781 agtcgcgcta aatttataag catcaaattt gttaaggtca ctaactttta ggtttattcc
      841 caaacattct gtatccatat ataaaggcaa ctcagtcgca ttaagttcac ggcgcggaat
      901 aaacttgacc tggcttattt ccaatcccgc aagatcatat ttcgccagag cgtttactaa
      961 ttcattagcc gacatccaag gcgctccgac gacgtgaaaa gggtgaggcg ttcctgtccc
     1021 cacactaata ttagtaaact gaaaaaaata caatccggca tagagaacag cagtctcttc
     1081 atcttgcacc gattcatccg caaatgacca tttcagtccg gtttgttgcc agggcatatc
     1141 gcgataataa tttgccattt taattacggt aagttgagat ttatctccgg cattaagcca
     1201 gttttcctca ttcattagca aagctaattc gcccaaagtc ataccgtgtc gagcaggtaa
     1261 agaaaagtca gccgcaaact tactctgaat tgacaccgga ccacctattt caactccgcg
     1321 taaaggattg ggtctatcaa gcacgatgat aggaattttt tgcttagcgc tgaactttaa
     1381 agcctcaatc aaaatcctga gcgcctctga ctcccgcaat ccgaaatcct gcaaatcgag
     1441 cacaatcatc tcggcgccat ttaacatact tgcactaagt cggtctctct cgctatttaa
     1501 aatataatat ttggtaattc cataaagctg actctgactt aattggaatc ctgagccggt
     1561 cagtaacgac cggtcatcca aaacctgtac acacgaccga cagcgcgttc ccaatcttat
     1621 attgacaatg tcaaaaatat acttatttcc ctgagcaaaa ctattgcgat cggcaatgac
     1681 cataaattta taattatcga gagcgtgaaa attatcatca attaacaaat ccaagccgat
     1741 tctaaagtcc tgcgtttctt catcaaccgg caaagtaatt accgtagaat gccgtaattt
     1801 ggcagtttga ccgtttattt cgctaaagac taaaagtaat aatattaatt cgccgaaaaa
     1861 tgaccgacgt ttatcccaaa ttattttcat aagcctgcta attctcgatc tttttctttc
     1921 caaaatccgg gtcaattttt accagactgg cgactaaaaa actgggaatc gtacaaaaca
     1981 ttatatagac aaagaaagtt ctatatccca tcaaatcctg caatttgccg ctgatcattc
     2041 ccggaagcat cattcccagc gccataaaag ccgttgcaat cgcataatgc gacgtcttat
     2101 attcaccttc cgccaaatac aacataaaga gcatatagcc ggtaaaaccg aaaccgtaac
     2161 cgaattgttc gatggcaaca caaattgtaa ctatcataaa attatccggc tgaacatacg
     2221 ataagtaaac atagactaaa tcaggaatat taatagccag cgccatccat aacacccatc
     2281 ttttcagccc gttgcgagcg gcaactattc caccgagaat gcctccgatt gtcagacaaa
     2341 ttattccgac ggttccatag acaaatccaa cctgcgtcgt tgtcagggct aatccgccag
     2401 cctcttggct atccagtaga aaaggcgaag ccatttttac caattgcgat tctgccaggc
     2461 gataaaacaa cagaaataat aaagccgaaa cgatgccttt tttccggaaa aaactggaaa
     2521 ataccatgaa ccagctggct ttgagttctt ggctttttgt tctgactgtc tccaatccgg
     2581 taggcaacat tactttgtga tacaaacaaa ttagcaggaa aaatacgcct aaaattccga
     2641 acgataccgc ccagctaaaa ggaatatcac cggcggtagc ttcaaattgc gacactaccg
     2701 gttctcgcag acggtgatca actttcaaag tcaccttgac cggcttattc caattctcat
     2761 tcgtaaattc caggcgactg gctgacgtca accagatgtc cttactacct ttcttgcggc
     2821 cgaaatttac tacaacggtt tcctcttttt tgggcggcgc tgacaggcaa ataaaaacat
     2881 tagcggaatc gctacctggc tcggctggat tatagatcgg cacttttact tccgccggaa
     2941 taacgactat attaggttgc tcagatgcct tgatcgctgg caaattattt tcatccagca
     3001 cttgaataat ttcattcggc ggaacagcca tagccgtaat actaacaggc tttagcccgg
     3061 ttttactcat aatataacca gttagcatca cgattaagcc ttgcccgacg atcattgcca
     3121 ttcggtaaaa cgtactgcga ataccgacga accaggtctg ctggtgcgca tccaagccaa
     3181 tcatataaaa tccatcagcg gcgatatcgt gagtcgccga ggctaaagca attatccata
     3241 aaacgatcaa cgtcaaagcg aaccagagcg gtaatcgaac gactagcgct accccgataa
     3301 aacaacatcc gagtataaat tgagtgatta atacccaatt gcgtttggtg gaaattacat
     3361 ctacaaatgg gctccagatt ggtttgatta cccagggcaa atacaaaaca ctggtccaaa
     3421 acgccaaagc cgcattagaa actcccatcg ttttgtacat aattaccgaa accgtcatcg
     3481 ctatgacgta gggaatgcct tcggcaaaat aaaggcttgg tacccaaagc cagggtgaac
     3541 gatttttcac agtcgccggt tcacttgcca ttacttttct cctgagtaat tcaatatgct
     3601 tggtggagtt taacacccgg ataataatgt tctaatattt ccaaggttga atagcctttc
     3661 agagccatcc cgagagcccc aatctgacaa taaccaaccc cgtgtcccca cccagctcct
     3721 ttcagaacaa aagccgccgg gatattttca gatccttcct ccacatcaac tacaaaagcc
     3781 gaactgtaaa ggaaaagttc atgaaatgct tcacgaatgg catattgatc attaatcacg
     3841 atttgcttgt caacattggc tttatcccga tagttaatcg ccagactggt cagtcgtccg
     3901 gacttgcccc gttttaatgg ttcgatgctc ttgatggcgg ccgcgtccag attcaatttc
     3961 agattgaata gcgccgtaat ctctctctgc ttcatccgaa aatgccagcg aaaataatga
     4021 ccttctttat caacattgcc aagatacttt ttcaactctt tttccggtac gatggatggg
     4081 caacaaaaag ccaacggcgg gttttcgatc caattgcgga ctttcgcctc tgtatcgagt
     4141 ggcaaagccg aatgtgggca atcaggcgga atatcaatca ctgaatccaa atatggcacg
     4201 gctgaaccat tccacacatt ttcaaaagat tcggtaactc cgccgcagtt cttgtgatag
     4261 cgggtatcac aaattttttc accgtatatt aagacctgac cggtagtttc aagcgcacct
     4321 ttgatcgaat gctctgaaag aaatgtcgtc ccctgatatc gctgacagca atcgtcattg
     4381 caaacgtcca ttccaagatt gcggtgtttc atttccacgt tcgccagcaa ccaactgcgg
     4441 gcgatgattg tctgcgattt gataacggat aatggacaag ccgctcccat ctcggaagtt
     4501 gccacgcaca ttaaatattg ctcaagcggc aatacattta tcatcattag attgtcattc
     4561 atccgactta tttcgattga gccgggcaga taaacatcaa tatatttttc ccaatgaaaa
     4621 ccacgtccgg caaccacatt cttgacttta atcccggctt tgttcgctat ctgaatttca
     4681 ccgcctctcg gaatgatttt ccaactcagt tgggcgttgg gtgttaattc attaatttga
     4741 atcccgattt ggttttcaaa taaaataaat tcaatctgtt cgccgccttt caaaatctgc
     4801 cgcgggccgc tctccggaat taattcataa actggagatt gtggaatcac gagcgcaatc
     4861 ctttgacatt tatcaattgg caggaaaatt cctacccgca ggattggttc ttcggtcgga
     4921 atcacattcg gttcaagcat aactttttct ccttccaggt taaataagcg tcaatagccg
     4981 cacccagcgc tggcgcttct cgcagagggg aaatttgaat tcgcgccagg tttaaatgct
     5041 tttgcggaca ataatgttct ttggctacct cgtcgaggac cgaagaattg ataattaatt
     5101 gagtcaattc attgacaaac ggcaaccaga gcacctcatc accttgggct tcgcacaata
     5161 attcacccaa tcgctgtccg atgaccagat tgtcaaataa cgccttttta taattatttt
     5221 cagtttgggg tcgtggacga tcgggattga caaagtcaaa tattccctgc caaccggcat
     5281 atatcgaggt aattcgttcg tagaaaagaa gtgccagaaa atgagcaact ttttgcatcg
     5341 taataaccgc cgctttttca ccatccaaag cccgacgacg gatttggggc ggaaagatac
     5401 cgttccgggt tagttcctct ggcgactttc cgattagctc gccataaatt gactgtattc
     5461 cactggaaga aacatacttt tccacggaaa agccgtctgg acacttcatt tcccaggttt
     5521 taacaaacca acccttggtt tcgtcaagcg gaatgacttt cccgtcgagt ttcagagcat
     5581 ccgccgcacc ggttccccca ccaaggtaat aagaattgcg aacattccga aacgaacctt
     5641 caccggcata ttcttcgccg attccacaat aataagcgtc actgccgatc tgattgatcg
     5701 gttgaactag ctctattcca gcaaaggtta tttcattttc gagtcgaacc gcataattgg
     5761 taatgcgcgg gccatttttc aggacagcga tgccgcggca atctctggtt ttgatacctg
     5821 gcataccaat tccaataagc acccgattgg tgccgctccg tttggtaatt tgttcaatac
     5881 accaggacgc ggctttgata aaacaattcg attgaacgag ttctgcggtc gtcggcacga
     5941 tttttcctgc ttcccactcg gcaatttgcc gatttaagtc aaccggctgg aaattatgga
     6001 tatagccggg aatttcagta taggactttg tcgcgtggaa attgccaagt gcaaaagcgg
     6061 ttccgtgatt ttcggctttc actgtccaac cgctgacttt ggttgcccct ccgtcaattc
     6121 caattaacaa aaattcatca tctgctatgt ctttcttaca attagccaat tttaaagtga
     6181 cttttccaaa ttacgattag tgatttcatt tatttttttc cgtcggtgaa cggctttcaa
     6241 acgcatatag tctttggctt cgtcgttgcg atcaatagta ttttggtcaa tgctatgatc
     6301 ggttccaccg gtatgccgca cgacttcata aatcggttgc cagactcgtc cgatgcgata
     6361 ttgctcactg atttttaaca ccaggtcata atcttcgcca taattgcaag caaacggttc
     6421 gtcgttcatc ccgaaataac ccatttcccg gataattttt atcggaatcg cccgtggagc
     6481 tccggcacca ttgatacgca agagattatt acgtccgttg gcatccgtcc actcttcgtg
     6541 tttgacaatc ggaatttctt ccattcggac aaattcacca cttggtttct tttcccagac
     6601 ttcgtacgaa ccgataacca ttccgattct ttcatcagaa ttgaagacag ctacaatttt
     6661 ctcaatcgca tctgatttta aacgatcatc agaatcaagc tggacgtagt agcgaccgcg
     6721 ggcaattttt aaaccaagat tcaacgatag tccaatattg ttaatatccg ctacgatcag
     6781 tctaaccggc ggttgctgag catcataacg gtctccaccc gccatatagc gccggacttc
     6841 agtaaccgtc ggatcaagaa tgcctccatt tacaacgacg ataatttcaa ggtctttgat
     6901 gctctggctg aagaccgaat tcagcgcatt accgataaat tccggccgat gataaaccgg
     6961 aattattacc gaagcgatgt agtcgtattt ttcgtttaga tccggccgca ggcaataatc
     7021 ttgatcaggt gcgagataag ctccgattct cttcaaatga ttagttaaaa ctacttcggc
     7081 ttccagctgg gcttctttgc tcaccaacaa ataatcaaaa acattatgtt taactccgcc
     7141 ggaaaccact cgataaagcg agccgtctat tttattggca atgtgaacca atttatattt
     7201 ttccgataac tttagacgaa tgtcatataa ggcgttgtat ttcaatgact gatcaaaacc
     7261 gccaactgct tccaaagccc ctttagtgaa caggaacacc ttgccataat cctgattatc
     7321 acgtaaacga ccgggatgat gtttcagaag tcggatttcc tgaatt
//
LOCUS       DAOOMU010000100         7315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_12046_length_7315_cov_6.397521, whole genome shotgun sequence.
ACCESSION   DAOOMU010000100 DAOOMU010000000
VERSION     DAOOMU010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12046_length_7315_cov_6.397521"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PLI55_08685"
     CDS             <1..713
                     /locus_tag="PLI55_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00945.1"
                     /translation="KNNLYLLYSGGDDLFIVGRWDKVIDFAALIREEFKKFTCHHPDI
                     SISGGIVLTPPKYPIHRSADLAGDAESASKSKRLINGNVKEKDAISFLGKTLSWNDFE
                     VAKALKDDLVQWIENPSEGKTKMNRGLINRLNQIYSTYQKEADLVKKQEKEGKIKIEE
                     VADRIQYNKWRWRLVYSLKRFLNQNENYNNEIENIQKALIDSNIYRGQLSEQPIIEYL
                     DLSVKWAEYQLRTNKKED"
     gene            715..1194
                     /gene="csm2"
                     /locus_tag="PLI55_08690"
     CDS             715..1194
                     /gene="csm2"
                     /locus_tag="PLI55_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HPB00946.1"
                     /translation="MEIENPKIDPNLVTIKKLTEFGKSNVKEWLRNGINKDSLKFCEE
                     FGFCLSRYCENEKPKPLSTNQIRNVYGEVKRIEMLLGSDPKEKEWKTVESKVLLLKPK
                     MAYSAKRDKSIPAKCLYEIVKASIEVVENSNLEEKITSFQNFAKLFEAIIAYHRAYD"
     gene            1231..1944
                     /gene="csm3"
                     /locus_tag="PLI55_08695"
     CDS             1231..1944
                     /gene="csm3"
                     /locus_tag="PLI55_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HPB00947.1"
                     /translation="MKLIKKIFIEGKITAQTGLHIGGSDVGLSIGGADSPVIRDPITN
                     VPYIPGSSIKGKIRSLLEKVDPTIQYNKLVGSARIHQCQVEEEKDVCLICRLFGLPAE
                     EEKANTPQRLIVRDAMMQPEWVDKLFKSKNTDMPYTEVKTEVVIDRVTSAANLRQIER
                     VPAGAVFELEMILNIYDSDIQDDLMKLLEKGRRLLNDDYLGGSGTRGYGKVNMTFAEI
                     KYKDEKIYQGDNQPLPLEG"
     gene            1945..2964
                     /gene="csm4"
                     /locus_tag="PLI55_08700"
     CDS             1945..2964
                     /gene="csm4"
                     /locus_tag="PLI55_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm4"
                     /protein_id="HPB00948.1"
                     /translation="MQFTIFKLKFRTPLHIGDNRPNYESCERFVHSDTLFSALINTWK
                     LLYPDDLDVFFPESAPASISFPYFRISSAFPFAGEEFFFPKPFYSPPYPQDGDSGDAK
                     TFKKLIWIGKNHLEKWLKGESLKIEDACLSPDKHFLFSDKIPKYQPNPVIVQDSPRIR
                     KDRETEQTEIFYYSRLYFAENAGLYFLATFQNEEWERKFNAVLNLLGDEGLGGDRTVG
                     NGLFEVIEKKEGFSLNLPEKSNHFLTLSLYHPKSGEMSKNLDNSYYELINRSGWVYSG
                     RGHSLRRNNVRMFSEGSVFLGSGNERGDVVKVLDPLPELNLPHAVYRYGQVLSLPISV
                     AEEAL"
     gene            2961..>3921
                     /gene="csm5"
                     /locus_tag="PLI55_08705"
     CDS             2961..>3921
                     /gene="csm5"
                     /locus_tag="PLI55_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HPB00949.1"
                     /translation="MNNKNITKKFRITVLTPVHIGTGFRKMKGIDFYFDERTSQVILV
                     DLDEIGTLLVDNSQALNELANLDEQNDIGDLTRRYHLNLKNNKNYPINSVGREFLEFV
                     RTGLGQPLIPGSSLKGSLRTAILRNIIDQKSDVERRELFKEIPNGGNPKSASDKLLKK
                     IFGSNPNYDLMRVLIISDSHFSEADLMLIESRVLSQNRNGWHWKPMPVWCEVLRPGTK
                     AEVGLSLDNFLLSDSIAVKKLNFPNRIPDDLNQFINQINHYSASMIGAEITYFEERDS
                     EKPELNEIIRNLNLVKKHIPANDSAMVIRLGWGSGWKHMTGDYL"
     assembly_gap    3922..4021
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4022..5464
                     /locus_tag="PLI55_08710"
     CDS             <4022..5464
                     /locus_tag="PLI55_08710"
                     /inference="COORDINATES: protein motif:HMM:NF021207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00950.1"
                     /translation="NVGNRDVKFDNQNIKVPRLSGKDYLDRFEKVAEHLTFPIITKGL
                     ECVFQDLQDIPLEQLILFYTDQDQNEVSEQHYQSDTLYFADLIEKLVNLRIPEKVKNV
                     VKIKIPHSPNDYDFTYEFFSFRLTEIENEVKPDRVYLSPAGGIPACNMNLLLQGSRIF
                     TRRAVTLLIPDNPNKPAKLQKLGEMINRENNRLIIQRLLSEYDFGGVAEVLENESDDK
                     SKSLLALARSVQYRLYFDFQTALNYLSNVNPEVLQIAETNIANFGTVENLINTIQENF
                     TALIKPIEPLHGNQTDFDQKNKWLELQKRLNAEVLLNVRIKWLSKQYIDFLGRIFRLQ
                     EGVLRWIFEKETGYSADSEQFPNDYPDFLKQNPDFEDYLKNESINGKNPVANRLNRGT
                     LLLYLRFLSETHAEYQPILKQCNKLDHLSALRNKCALAHGNEPVNDCIISQIYGKNVL
                     GNLSKLASTFGLEDHLPQIDCIRNIIIENF"
     gene            5669..6643
                     /gene="cas1"
                     /locus_tag="PLI55_08715"
     CDS             5669..6643
                     /gene="cas1"
                     /locus_tag="PLI55_08715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HPB00951.1"
                     /translation="MAFIYFTEQGSKLLKSGNRLIVEKDGEKIADIPLIKIETILLYG
                     NIQITTPALKMFMDEGIDIAFFTMNGKLCGILNPIKSKNIILRLKHFELARNPDFALK
                     IAKSLVSAKIKNMQAVLKAYLKNYSDGEIANAVQDLKLRLAGIAPKCKAANLLGVEGS
                     STQVYYSALRRMFRGELRFNGRNRQPPRDEVNALLSFCYVILGNEITMLLNGCGLDPY
                     LGFYHGIQYGRASLAMDLLEPFRPAIDRFVLSLANLGIFKKADFEQREGGIYLTQSSM
                     KRFFRHWQEFLTVSSGSHKSTRDNIYSQIESLIKGLNQSGEFEPFLIE"
     gene            6653..6928
                     /gene="cas2"
                     /locus_tag="PLI55_08720"
     CDS             6653..6928
                     /gene="cas2"
                     /locus_tag="PLI55_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPB00952.1"
                     /translation="MFYVVSYDIKDDKRRLKIAKTLLDFGTRVQYSVFECNLNDKQIQ
                     KLQDKINNLIDNRADTVRIYSLCEACHHRVTIHGTGVLTEDADLYVF"
BASE COUNT         2316 a         1344 c         1461 g         2094 t
ORIGIN      
        1 ttaaaaacaa tctgtattta ctctattccg gcggtgatga cctttttatt gtgggacgct
       61 gggacaaagt aatagacttt gccgctttga ttcgagaaga attcaaaaag tttacctgtc
      121 atcatcctga tatatcaatc tccggaggta tcgtactgac gccgccaaaa tatcccattc
      181 atcgttccgc tgatttggct ggtgatgcgg agtcagcctc caaatccaaa cgtcttatca
      241 acggtaacgt aaaggaaaaa gacgcaataa gtttcctcgg caagactctt agctggaatg
      301 atttcgaggt agccaaggct cttaaagatg atttggttca gtggatcgaa aatcccagcg
      361 agggtaaaac taaaatgaat cgaggtctta ttaaccgcct caatcaaata tactcaacct
      421 atcaaaaaga agcggatctg gtaaaaaaac aagaaaaaga agggaaaata aaaatcgaag
      481 aagtcgctga tcgaattcaa tataataaat ggcgctggcg tctggtctat tctctcaaac
      541 gcttcctaaa ccaaaatgag aattacaata acgagattga aaatatccaa aaggcactga
      601 ttgatagtaa tatatatcgc ggccaactaa gcgaacaacc cataatcgaa tatctcgatt
      661 tgtccgtaaa atgggcagaa tatcaactaa gaaccaataa aaaggaggat taaaatggaa
      721 atcgaaaatc caaaaattga tccgaattta gtaactatta aaaagttaac agaattcggg
      781 aaaagcaatg tcaaggaatg gctaagaaat ggcattaata aggattcatt aaaattttgt
      841 gaagaatttg gattctgcct atctcgatat tgtgaaaatg aaaaaccaaa gccactatca
      901 acaaatcaaa tcagaaatgt gtacggtgag gttaaaagaa ttgaaatgtt attaggtagt
      961 gatcctaaag aaaaagaatg gaaaacagta gaatctaaag tgctattact caaaccaaaa
     1021 atggcctatt cagcaaaaag agacaaatca attccagcta aatgccttta tgaaattgtt
     1081 aaggcaagta tcgaagttgt tgaaaattca aatttggaag agaaaataac ttcctttcag
     1141 aattttgcaa aattatttga ggcaataatt gcctatcatc gtgcttatga ttaaagtatg
     1201 tagaaataat aattaaagga gaaaaataaa atgaaattaa ttaaaaaaat tttcattgaa
     1261 ggtaaaatta ctgctcagac tggactgcat attggcggct cggacgtcgg attatccatc
     1321 ggtggtgccg attccccggt aattcgcgat cctattacta atgtccccta tatcccgggc
     1381 tcatcaatca aaggtaaaat tagaagcctt cttgaaaaag ttgaccctac tattcagtac
     1441 aacaaactgg taggaagtgc cagaattcat caatgtcaag ttgaagaaga aaaagacgta
     1501 tgcctgattt gtagattgtt tggtttaccg gcagaagaag agaaggcaaa cactccgcaa
     1561 cgtctcatcg ttcgagatgc tatgatgcaa cccgagtggg tggataaact ctttaagagt
     1621 aagaataccg atatgcctta tactgaagta aaaactgaag ttgtcattga tcgggtcact
     1681 tctgccgcta atctacgaca aattgaacgt gttccggctg gcgcagtctt cgaattagag
     1741 atgattctta atatctacga tagtgatatc caagatgatc ttatgaagtt acttgaaaaa
     1801 ggccggcgcc tattgaatga tgattatctc ggtggtagtg gaacgcgtgg ttatggtaaa
     1861 gtcaatatga cctttgccga aatcaaatat aaagatgaaa aaatctatca aggagacaat
     1921 cagccactcc cactggaggg ataaatgcag tttacaattt ttaagctcaa atttcggacg
     1981 ccactgcata tcggcgacaa ccgaccgaat tacgaaagtt gcgaaagatt tgttcatagc
     2041 gatacgctat tcagtgccct gataaatacc tggaaactgc tttatccgga tgatcttgat
     2101 gtctttttcc ccgaatccgc tccggcatcc atttcttttc cctattttcg gatttcatcg
     2161 gcttttccat ttgccggcga agagtttttc tttccgaagc ctttttacag tccaccgtat
     2221 ccgcaagatg gcgattctgg cgatgccaaa acctttaaga aactgatctg gattggaaaa
     2281 aatcatctgg aaaaatggct caaaggcgaa tcgttaaaaa tcgaggatgc ttgtctttcg
     2341 cctgataaac atttcctctt ttctgacaaa ataccaaaat accagcctaa tccagtgatc
     2401 gtgcaggatt cgccgcggat tagaaaagat cgggaaaccg agcagaccga aatcttctat
     2461 tattctcgct tgtattttgc cgaaaatgcc ggcctctact tcctggcaac cttccaaaat
     2521 gaagaatggg aaagaaaatt caatgccgtt ctgaatttac tgggtgatga aggactcggc
     2581 ggcgatcgca cggttggaaa tggacttttt gaagttatcg aaaagaaaga aggattctca
     2641 ctcaatctac cggaaaaatc aaatcatttt cttacacttt cactttatca tccaaaatca
     2701 ggagaaatgt ctaagaatct ggataattct tactacgaac ttattaatcg tagtggctgg
     2761 gtttactctg gtcgtggtca ttcgttaaga cgtaataatg tccggatgtt ttcggaaggc
     2821 tctgtatttt taggttcggg aaatgaacga ggcgatgtcg tcaaggttct cgatcctctg
     2881 ccggagctga atctaccgca tgccgtttat cgctatggcc aggtcttgag tctgccgatt
     2941 agcgtcgcgg aggaagcact atgaataaca aaaacataac taaaaagttt agaattaccg
     3001 tcctgacgcc ggttcatatc ggtactggtt tccgcaaaat gaaaggcatt gatttctact
     3061 tcgatgaaag aactagccag gttattctgg tagatttaga cgaaatcggg acacttctgg
     3121 tagataattc tcaggctttg aatgaattag ccaatctcga tgaacaaaac gatattggtg
     3181 acctgactcg acggtaccac ctgaacttga aaaataataa gaattatcca attaatagtg
     3241 tcggaagaga atttctggag tttgtccgca ccggcctcgg tcagccgctg atccccggca
     3301 gctccctgaa aggttcttta cgtacggcta tcttgagaaa tattattgat caaaaaagtg
     3361 atgtggaacg tagggaacta tttaaggaaa taccaaacgg agggaatcca aaatcggcgt
     3421 ctgataaatt acttaaaaag atttttggta gtaatccaaa ctacgatcta atgcgcgttc
     3481 tgataataag cgactcccat ttttcggaag ccgacctgat gctaattgaa tcacgcgtcc
     3541 tatctcagaa tcgtaatggc tggcattgga aacctatgcc ggtctggtgt gaagttctgc
     3601 gtcctggtac taaagctgaa gtcggcttgt cactggataa tttcctgcta tctgattcaa
     3661 tagccgtaaa aaaattgaat tttcctaata ggattccaga tgacctgaac caatttatta
     3721 atcaaattaa tcattattca gccagtatga ttggtgctga gatcacctat tttgaagaga
     3781 gagatagcga aaaacctgaa ttgaacgaga ttatcagaaa tcttaatctg gtcaagaaac
     3841 atattcccgc taatgattcc gctatggtta tcagactcgg ctggggtagc ggctggaaac
     3901 atatgaccgg tgattattta gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 naatgtcggt aatcgagatg tgaagtttga taatcagaac attaaagtgc ctcgcttatc
     4081 cggtaaagat tatctagacc ggtttgaaaa agtcgctgag catttaacat ttccgattat
     4141 cactaaggga ctagaatgtg tattccaaga cttgcaagat atacctttag agcagttaat
     4201 ccttttttat accgatcagg atcaaaatga agtatccgag caacattatc aatcggacac
     4261 tctctatttc gccgatctta tcgaaaaact tgttaatctt agaataccgg aaaaagtcaa
     4321 gaacgttgtc aaaataaaaa tacctcactc tcctaatgac tatgacttca cctatgagtt
     4381 cttttcattt cgacttactg aaatcgaaaa cgaagtcaag cccgaccgcg tctatctttc
     4441 tccggcaggt ggaattccag cttgcaatat gaatcttctc ttgcaaggta gtcgtatttt
     4501 tacgcgacgc gcggtaactc ttcttattcc tgacaatccc aataaaccgg ccaaacttca
     4561 gaaactcgga gaaatgatca atcgggaaaa taatcgcttg attatccaac gtcttttaag
     4621 tgaatatgat tttggtggcg tagcggaagt actggaaaat gaatccgatg ataagtcaaa
     4681 atcgcttctg gcgctggctc gttcggttca ataccgactc tatttcgatt tccaaacggc
     4741 tttgaattac ctgtctaatg tgaatcctga agttctacaa attgccgaaa ctaatatcgc
     4801 caattttggc accgtcgaaa atctcataaa tactatccag gaaaatttca cggcgcttat
     4861 caaaccaatt gaaccactcc atggtaatca aactgatttt gatcaaaaaa ataaatggct
     4921 ggaattgcag aaaagactga atgccgaggt tctactgaat gtcagaatca aatggctttc
     4981 taaacaatat attgatttcc ttggtcgaat atttcgtctg caagaaggtg tcctgagatg
     5041 gatttttgaa aaggaaaccg gatattcagc cgactctgag cagtttccca atgattatcc
     5101 tgattttctt aagcaaaatc ctgatttcga agactatttg aaaaatgaat caatcaatgg
     5161 caaaaatcct gttgctaatc gcttaaatcg gggtactttg cttctttatt tacgatttct
     5221 cagcgaaact catgctgagt atcagccgat tttgaagcaa tgcaataaat tggatcactt
     5281 atcggctctg cgcaataaat gcgccctggc tcatggcaat gaaccggtaa atgattgcat
     5341 catttcccag atatatggta agaacgtatt ggggaatctt tccaaattgg caagtacatt
     5401 tgggcttgag gaccacttgc cccaaattga ttgtatcaga aatattataa tcgaaaattt
     5461 ttgatttttt cgctctttga cattgtgata aaaaaatagt tctctgtaag tgaatttttt
     5521 atgaaccgtg gcgaaggcat cggcggctta aggaggtctc tgctcaattg actgggagtt
     5581 atatttcctt tttaataatc cgattttgaa gaacttattc cgatagtagt ttcagaagag
     5641 tcttatctaa aaaatagggg gtttgaaaat ggcttttatc tattttactg aacaaggttc
     5701 aaaattactc aaatcaggta atcggctaat cgttgaaaaa gacggggaaa agatagccga
     5761 tattccacta attaaaattg agacgatttt actctatggc aatattcaga tcactactcc
     5821 ggccttgaaa atgtttatgg atgagggtat agatatcgct ttcttcacta tgaacggtaa
     5881 attgtgtggt attcttaatc ccataaaatc aaaaaatatt atacttcgtc ttaaacactt
     5941 cgaactagcc cgtaatccag attttgctct gaaaatcgcc aagagcttag tttctgctaa
     6001 aatcaaaaat atgcaagcag ttctaaaagc ttatctaaaa aactattctg atggagaaat
     6061 cgcaaacgca gtccaggatt tgaaattacg cctagccggt atcgctccta aatgtaaagc
     6121 cgctaatctc ctgggtgtgg agggttcgtc tacacaggtt tattacagtg ccttgcgacg
     6181 tatgtttcgg ggtgaacttc gcttcaatgg acgcaaccgc caaccaccgc gcgatgaagt
     6241 caatgctttg ttgagctttt gttatgtaat attgggaaat gaaattacca tgttgctgaa
     6301 cggatgtgga cttgacccct atttgggttt ttatcacggt attcaatatg gtcgggcttc
     6361 tcttgcaatg gatttactcg agccatttcg gccggcaatt gaccgctttg tattaagttt
     6421 ggctaacctg ggaatattca aaaaggctga ttttgaacaa cgcgagggtg gaatttactt
     6481 gactcagtca tctatgaaac gcttttttcg tcattggcaa gaatttttga ccgtttcctc
     6541 aggcagtcat aaaagtactc gagataatat ctatagtcag attgaaagcc ttattaaggg
     6601 cttaaatcaa tccggcgaat ttgaaccttt cttgatagaa tgagagattc gtatgtttta
     6661 tgttgtttct tatgatataa aagatgataa gcggcgcttg aaaattgcca agactctgtt
     6721 agattttggc acacgagttc aatattcggt atttgaatgt aatctaaatg ataaacagat
     6781 tcaaaaattg caagataaaa tcaataactt gattgataat agagctgata cagtccgcat
     6841 ctatagtcta tgtgaagcct gccatcaccg agtaacgatt catggtaccg gagtcttaac
     6901 tgaagatgct gatctgtatg ttttttgata accgtaacct taaaattggg tttaaaagac
     6961 ttaggctacg aaaattaata taccaaattt aatttccttg tgttatgaac acaataaacc
     7021 gaaaaaaaag tataaagaaa ggctatttag gatttatatt tggactggtt acgaaattac
     7081 tttgaaaaaa gaattgaaaa tcaggtggtt actaaatgtg ctgtgataat cattgacccg
     7141 atgataaggg gattaagaca tccaggcgct tttcgatgcc agtgccttta gatcaggtga
     7201 taatcattga cccgatgata aggggattaa gacaccagct tctaattcag gcagaatatc
     7261 tgtttccgtt acgtgataat cattgacccg atgataaggg gattaagact catag
//
LOCUS       DAOOMU010000101         7135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_12458_length_7135_cov_20.663136, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000101 DAOOMU010000000
VERSION     DAOOMU010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 7135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7135
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12458_length_7135_cov_20.663136"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="PLI55_08725"
     CDS             <1..588
                     /locus_tag="PLI55_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB00953.1"
                     /translation="SIILDIGAGNGSFAKFIGRNDVFMIDGNPETVVNNQKFFPNYQY
                     VHLPDLPFQNNKFDIIHCSHVIEHLPPNDFFLLLKNCDRCLKISGYIIISSPLMWYHF
                     WDDLSHVRPYSPRIFEKYLCEKSSPNSTREKISNKYSIIELQYRYHLTEIGADVINTR
                     SSFVNQVYRIVMQFLTRVGFRRIEKTGYTIVLKKS"
     gene            598..2526
                     /gene="asnB"
                     /locus_tag="PLI55_08730"
     CDS             598..2526
                     /gene="asnB"
                     /locus_tag="PLI55_08730"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HPB00954.1"
                     /translation="MCGICGIYHHGKIEVSPAEICRMRDFMIERGPDSSGYELLPEIA
                     LGHRRLKIIDLSDAATQPMTNADRSIWTVFNGAIYNYRELRQELLAVGYHFKSNSDTE
                     VLLQGYAEWGTDLFQKITGMFAIAIYDSQKKQLILARDRVGKKPLYFTEQSGIVYFAS
                     DIKAVVAVLPAQPPVSLTALDCYLHHIAIPDQHTIFEGIQKVQPGSYHVFSENGQVVT
                     TYWEWNYAHKVVNDEPIILQNCEQLLIQAVLRRTVGDVPIGTMLSGGVDSSIITAILC
                     RNSTQRIKTFTMGFKNYPMEDILAAREVAKLYDTEHTELILDHDVTENLLELVWQFGE
                     PFADSSAIPTYLISRAARQYITVMLTGDGGDEAFGGYGRTIVPRNAQIFSQIIPPFLH
                     PLVGGILKGIGVNPESNSIFGKILFYINYLKGFPYESFYNTMGFYKYRNSLWSPEYLS
                     KIAGHNPLHPFHENFRKVSDLDPIDQVLFTDGMTRLVYDYLVKIDRATMANSIECRSP
                     FLDTALLEYVAKIPPKMKFKNHQTKYLLKKIAEKYLPHKLIYTEKRGFGVPVDRWIQN
                     DLSGIFETVVFNRYARQRNYFNYDFINKIWNGQRTNVQNHKHRLWALFWLELWHLMFI
                     DKIISRDMPLREVERILK"
     gene            2523..3680
                     /locus_tag="PLI55_08735"
     CDS             2523..3680
                     /locus_tag="PLI55_08735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB00955.1"
                     /translation="MKQINVLHIIGSLQLGGAEKSLLLLLQNIDRAKFNPVVVTMYSD
                     GLNDFFSDDFRKLNIPLYHLHLKSWRDIETFRGLRQIIRRHRIDIAHSHYGALEFFGT
                     LYARLAGVRHCVYTKHNLRFKTSLLFKLGRILLNRILAERILSISKTVTEHLVNTEYA
                     SRSRIRLVYNPVENPKIPNAAHQASIKQKWQIPTGQFIIGNTSRFDSSKGFDIFYLVL
                     KYLRKMGIPVHAVVMGNESSQMEHQRLISENDLHELITILPYQKDLSPVYSILDCYLF
                     PSIHTEGFGIALLEAMSYGLPVVGLNVGVMNEIIRNQSNGLLPYPEKWEKTFHGNRTD
                     AAKALAKAIAELYNSPVLLRNISANAKVTAEKYSVTNFVNKLEAVYLELFK"
     gene            3677..4825
                     /locus_tag="PLI55_08740"
     CDS             3677..4825
                     /locus_tag="PLI55_08740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB00956.1"
                     /translation="MKILVLTDLFPTLSNPNHGIFIYQWAYHLAQSCDLTVYQIIFKE
                     NQQSVSELELKRFRENYDANQRFAWTQRIKTIGRFDRIWRRSRQFYRQSKLDLSQTIG
                     EFDIIIGQMGCPGGYAAVRLAKKFGKKSIVGLRGSDVNAYLSRPVLKHYMHWTLKNAD
                     ILVTVSEAMKVELIKRGYPESKINVIYNGVDEKLFHPLDQVEAQKKLKLPPNYKYLLF
                     VGTLSQRKGINLLLSAFAELNRPEVKLILIGQGNDRHLIENFRIEHSLQDRILLSGVI
                     PHAQLPLWYAASDVVVLPSLSEGVPNVILEALAIGKPVVASQVGGIPEVLAGNKAAFL
                     CQPGDLNSLFEAIKKALNYSSKIEPFHKFTWEMNTKQFLQIFQEVIRR"
     gene            4822..5994
                     /locus_tag="PLI55_08745"
     CDS             4822..5994
                     /locus_tag="PLI55_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB00957.1"
                     /translation="MKSNSTQIIFYFCFEGFSRLKDINNHIRETVSWLAKFGYEVHFF
                     NPNIVKPSFSSKVHIHLIPVINYPFLRWATFEFLSFFILTWNALKRRPTHLYFRETSS
                     FTPLLVSKLCHIPLTIEVNGWVLEELRQIGYSRWKLAYIRLNQRLNFVHCNRIIPVTE
                     GLKNLIVTNYPVKSVKIIPIANGTNPEVFRPMPKSEALAKTGLPNTPTVGFIGSCYHY
                     HGVQYLIKAAPLVLQKMPQVRFVVAGDGAQLEAWKTLSRELKVNEAFTFTGKVPFSLA
                     PYYINSFDICVAPWDTDLLPKDGQSPMKFFDYLACGKPVIASPVSSVVKLINKFRCGI
                     TCDVKNSEVFAALIIELLSNPKIREPLEKTAREVVTANFTWEITSRKIEQVLKGIR"
     gene            5994..>7135
                     /locus_tag="PLI55_08750"
     CDS             5994..>7135
                     /locus_tag="PLI55_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00958.1"
                     /translation="MYDQFVRDFYFPLTQHIKGEKIAQYLKILRYNEHLPLEKLQKIQ
                     LAKLHHILNVAYQNVPYYQAIFNQLGIKPEDISLPQDFAHLPLLNKKDIRENLSRLTN
                     PNYSGKIYSGKTSGSTGIPLKFYITNEYSSWDWASRWRARNWFGVQIGDPEVAIWGRP
                     LHSTIRKVIDALKGRIRNTLLISGFEYSEEMLEKLSRQIQRFNPCYFYGYSNSIYRLA
                     LYYKENNLPLPGRLKAIFVTAETLFPQERKIVESVFHVPVSNEYGCSEIGGFAYECPA
                     GNWHVSLENVYLEFIENENGVREIVGTSLTNEYMPFIRYRIGDVGEWINGKCPCGCNL
                     PLMKLHIGKATDIVVMKNGQTFSSEIFDYLNLALLDLNRQPFDQYQ"
BASE COUNT         2077 a         1587 c         1460 g         2011 t
ORIGIN      
        1 tcaataatcc tcgatattgg ggcaggaaac ggttcttttg ctaaatttat tggcagaaat
       61 gacgtcttta tgattgacgg aaatcctgaa acagttgtca ataaccaaaa atttttcccg
      121 aactatcagt acgtccactt accggactta cctttccaaa acaacaaatt cgatatcatt
      181 cattgtagcc atgtaattga acacctgccc cctaatgatt tcttcttact actaaaaaat
      241 tgcgatcgtt gtctgaaaat tagtggttat ataattattt ctagcccttt aatgtggtac
      301 catttctggg atgatttatc ccatgttcgg ccttattctc ccagaatctt tgaaaaatac
      361 ctctgcgaaa aatccagccc taactctacc cgagaaaaaa tttctaataa atattctata
      421 atagaattac aatatcgata ccatctgaca gagattggcg cagatgtcat taacactcgc
      481 tcttcatttg tcaatcaagt ttaccgaatt gttatgcaat ttttaactcg agttggtttt
      541 agaaggatag aaaaaaccgg ttacacaatt gtcctaaaga aatcgtgaat tttaattatg
      601 tgtggcatct gcggaatata tcatcacggt aaaatcgaag tcagcccggc ggaaatctgc
      661 cggatgcgcg actttatgat cgagcgtggt cccgactcat cgggatatga acttctgccg
      721 gaaatcgctc tcggtcatcg ccgccttaaa atcattgacc tatcggatgc cgccactcag
      781 ccgatgacca atgcagatcg tagcatctgg acggttttta acggtgcaat ttataactac
      841 cgcgaactcc gccaggaatt actcgcagtc ggttaccact ttaaatccaa ttccgatact
      901 gaagtgcttt tgcagggtta cgcggagtgg ggaaccgatt tattccaaaa aattaccggc
      961 atgttcgcca ttgctattta cgattctcag aagaaacaat taatcctggc tcgcgatcgt
     1021 gttgggaaaa agccactcta cttcaccgaa cagtccggta ttgtctattt cgcatcggat
     1081 attaaagcgg tggttgcggt tttaccggct cagccaccgg tcagcctgac ggctttggac
     1141 tgctacctgc atcatattgc cattcctgat caacacacaa tcttcgaagg aattcaaaaa
     1201 gtccagccgg gatcttatca tgtattctcg gaaaatggtc aggtcgtcac gacctactgg
     1261 gaatggaatt acgctcacaa agtcgtgaac gatgaaccta taattttgca gaactgtgaa
     1321 caattattga ttcaggctgt attgcgccgc acggtcggcg atgttccaat cggtacgatg
     1381 ctcagcggcg gagtggattc gagcattatt accgctattc tatgccggaa ttccacccag
     1441 cggatcaaaa catttacgat gggttttaag aattacccga tggaagacat tcttgccgcg
     1501 cgggaagtcg ccaaattata cgataccgaa cacaccgaac tgattttaga tcacgatgtc
     1561 actgaaaatc tactggaact cgtctggcag ttcggtgaac cgtttgccga ttcttcggcg
     1621 atccccactt atttaatttc cagagctgcc cgccagtaca ttaccgtaat gttgactggt
     1681 gatggtggcg acgaggcttt tggcggttac ggccgaacaa ttgtgccacg caatgctcaa
     1741 attttttccc aaataattcc acccttcctg catccactgg taggcggaat tttaaaaggt
     1801 atcggagtaa atccagagag caactcgatt ttcggcaaga ttcttttcta cataaattat
     1861 ttaaaaggct tcccgtacga aagcttttat aatacgatgg gattttataa atatcggaat
     1921 tctctatgga gtccggaata tttgtcaaaa attgctggac ataatccgct tcacccgttt
     1981 cacgaaaatt tccgaaaagt aagtgacctc gatcccatag atcaggtgct gtttacggac
     2041 ggtatgaccc ggctggttta cgattattta gtaaagatag accgggcgac catggcaaac
     2101 tctatcgaat gccgctcacc ctttctcgac acggctttac tggaatatgt cgccaaaatc
     2161 ccgcccaaaa tgaaatttaa aaaccaccag accaaatacc tgttgaagaa aatcgccgaa
     2221 aaatatctac ctcataaact catttacacc gagaaacgcg gattcggtgt cccggttgac
     2281 cgctggattc aaaacgactt gagtggaatt ttcgaaacgg tcgtattcaa ccgttacgcc
     2341 cggcaaagga attattttaa ctatgacttc attaataaga tttggaatgg ccagcgcact
     2401 aatgtccaaa atcataaaca tcgtctttgg gcactctttt ggctggaact ctggcattta
     2461 atgttcattg ataaaatcat tagtcgcgac atgccgctca gggaagtcga acgaatactg
     2521 aaatgaaaca aatcaacgta ctgcatatta tcggaagttt acagctcggc ggcgccgaaa
     2581 agtcactact tttgcttttg caaaatattg accgtgcaaa attcaatcca gtggttgtta
     2641 cgatgtattc cgacggtttg aacgattttt tctctgacga tttccgcaaa cttaatatcc
     2701 cgctctatca cctgcattta aaatcctggc gagatattga gacttttcgg ggattgcgcc
     2761 aaattattcg gaggcaccgc atcgacatcg cccattcaca ctatggcgca ctggaattct
     2821 tcggaacact ttacgcgcgg ctggccggcg tccggcattg tgtttatacc aaacataatc
     2881 tacgcttcaa gaccagcctt ttattcaaac tcgggaggat attgcttaac cgcattctgg
     2941 cagagcggat tttatcaatc tcaaaaaccg tcactgaaca tttagtaaat accgaatatg
     3001 cttcccgaag ccggatcagg ctcgtttata atccggtcga aaatcctaaa ataccgaatg
     3061 ccgcccatca ggcttcaatt aaacagaaat ggcagattcc aactggacaa tttatcatcg
     3121 gaaataccag tcgctttgac tcgtccaaag gtttcgatat tttctaccta gtgctgaagt
     3181 atctgcggaa aatgggcatt ccggtacatg cagtggtaat gggcaatgaa tccagtcaga
     3241 tggaacatca aagacttatc agcgagaatg atcttcacga attaataaca atcctgcctt
     3301 atcaaaaaga cctgagtcca gtctactcga tcctcgattg ttatctattt ccttcgattc
     3361 ataccgaagg atttgggata gccttactag aggcaatgag ttacggattg ccggtcgtcg
     3421 gattgaatgt gggcgtaatg aacgagatta tccgcaacca gtctaatggg cttttaccgt
     3481 acccggaaaa gtgggagaag accttccatg gtaatcggac cgatgcggca aaagctctgg
     3541 ctaaggccat tgccgaatta tacaatagcc cagttttact tcggaatatt tctgcaaacg
     3601 caaaagtaac ggctgaaaaa tattcagtca ctaattttgt taacaaatta gaagcggtat
     3661 atttggaatt attcaagtga aaattctggt actcactgat ctcttcccga cactctcaaa
     3721 tcccaaccac gggattttta tttatcaatg ggcatatcat ttggcgcaaa gttgcgacct
     3781 gacagtttac cagatcattt ttaaggaaaa tcagcaatca gtcagtgaac tggaactgaa
     3841 gcgtttccgt gaaaattacg acgccaatca gcgctttgcc tggactcagc gcataaaaac
     3901 aatcggtcgc tttgatcgta tctggcgacg ttcgcggcaa ttttatcgtc agtcaaaatt
     3961 ggatttatcc caaacaatcg gtgaattcga tattatcatc ggacaaatgg ggtgccccgg
     4021 cggttacgcg gcggtgcgat tagcaaagaa attcggaaaa aagtcaatcg ttggtctgcg
     4081 cggttcggat gtcaatgctt acctgtcgcg gccagtctta aagcactata tgcactggac
     4141 attgaagaac gctgacatcc tggtcacagt ttctgaagct atgaaagtag aactaattaa
     4201 aagaggatat ccggaatcaa aaattaacgt tatatataac ggtgtggacg aaaagctttt
     4261 ccatccgctt gatcaggtag aagcccaaaa aaaattaaaa ttgccgccaa attataaata
     4321 cctgcttttt gtcgggacgt tgagccaacg caaaggaatc aacctattac tttcggcatt
     4381 cgcggaactt aatcggccag aagtaaaatt aattttaatc ggtcagggca acgaccgcca
     4441 cctgatcgaa aatttccgta ttgaacattc actacaggat agaattctcc tatcgggagt
     4501 cattccgcat gcgcaattac cactttggta cgccgcgtcg gatgtagtag tactaccgag
     4561 tctttcggaa ggcgtcccga acgtaattct ggaagcactg gcaattggca agccggtggt
     4621 agcctcgcag gtcggaggta ttccggaagt attagctggc aacaaggcag cttttctatg
     4681 ccagccaggc gatttaaatt ccctgtttga ggctatcaaa aaggcactca attattcaag
     4741 taaaattgaa ccttttcata aatttacctg ggaaatgaac accaaacaat ttctacaaat
     4801 atttcaagaa gttatcagga gatgaaatcg aattctaccc aaataatctt ttacttttgc
     4861 ttcgagggtt ttagtcgctt aaaagacatc aataatcaca ttcgtgaaac ggtcagctgg
     4921 ttggcgaagt ttggatacga agtgcatttc tttaatccaa atatcgtcaa accaagtttc
     4981 agctctaaag tccatattca tttaatcccg gtcattaatt atccattcct acgctgggca
     5041 acctttgagt ttttatcttt ttttatttta acctggaatg cacttaagag acgaccaact
     5101 catttatact ttcgcgaaac gtcatcattc actccactgc ttgtctcaaa gctctgccac
     5161 ataccactaa ctatcgaagt caacggttgg gtattggaag aactccgtca aatcggttac
     5221 tcgcgctgga aactcgccta cattcgtttg aatcagcgcc tcaattttgt tcactgcaat
     5281 cgtatcatac cggttactga gggtttgaaa aacctgattg ttactaatta tccggtcaaa
     5341 tccgttaaga tcattcccat cgcaaatggc acgaatccgg aggtctttcg gcctatgccg
     5401 aagtcggaag ctttagccaa aaccggcttg cccaatacgc cgactgtcgg atttattgga
     5461 agttgttatc attatcacgg tgtacaatat ctaatcaagg ctgctccact tgttctccaa
     5521 aaaatgccac aagttcgctt cgtggttgct ggcgacggtg ctcaattaga agcatggaaa
     5581 accttgagcc gtgaactgaa agtaaatgaa gcttttacat tcaccggcaa agtccctttt
     5641 agtttggcgc cctattatat caattcattt gatatttgcg ttgcgccgtg ggatactgat
     5701 ctactaccaa aggacggaca atcgccgatg aaattttttg actacttggc atgtggcaaa
     5761 ccagtcattg ccagcccggt ttccagcgtg gtcaaattga tcaacaaatt tcgttgtgga
     5821 attacctgcg acgtaaaaaa ctcagaggtt ttcgccgcgt taattattga gcttttatct
     5881 aatccaaaaa tccgtgagcc attggaaaaa accgctcgcg aagttgtcac cgcgaatttc
     5941 acttgggaaa tcactagtcg taaaattgag caagtattaa agggaattag ataatgtatg
     6001 accagtttgt ccgggacttt tatttcccct tgactcagca cattaaaggt gagaaaattg
     6061 ctcaatattt aaaaattctt agatacaatg aacatttacc gctggaaaaa ttgcagaaaa
     6121 tccagctggc taaacttcat catattctta acgtagctta tcaaaacgta ccctattacc
     6181 aagcaatatt taaccagctt ggtatcaagc cagaggacat ctccttgccc caggatttcg
     6241 cccatcttcc gttgttaaat aaaaaagata ttagggaaaa tctcagtcgt ttaacaaatc
     6301 caaattattc cggtaaaatt tattctggaa aaactagtgg ctcaacgggt attccattga
     6361 aattttacat tactaacgaa tatagcagtt gggactgggc tagtcggtgg cgagcacgga
     6421 actggtttgg agttcaaatc ggggatccgg aagttgctat ctgggggcga ccgctccact
     6481 cgactattcg caaagtcata gatgccctga aaggacgcat tcggaataca ctgctaattt
     6541 cgggctttga atactcagaa gaaatgctgg aaaaattaag tcgtcagatc caacgcttta
     6601 atccgtgcta cttttatggt tattctaaca gcatttaccg gcttgcactc tattataaag
     6661 agaacaacct gccacttcct gggcgcttaa aagcaatttt cgtaaccgca gaaactctat
     6721 ttccacagga acgcaaaata gtcgagtcag tttttcacgt tccagtttct aacgaatatg
     6781 gttgttcaga aatcggtggt tttgcttacg aatgtccggc tggtaattgg cacgtatctc
     6841 tggaaaacgt atatctcgaa tttatcgaaa atgaaaacgg agtccgcgaa atcgtcggaa
     6901 cttcgctgac taatgaatac atgcctttta ttcgctatcg cattggtgac gtcggcgaat
     6961 ggattaacgg aaaatgccca tgcggttgca atctgccact catgaaattg cacatcggaa
     7021 aagccactga tattgttgta atgaaaaacg ggcaaacttt ttcaagtgaa attttcgatt
     7081 acctgaatct ggctttactc gatctgaatc ggcaaccttt tgatcagtac caaat
//
LOCUS       DAOOMU010000102         6948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_12891_length_6948_cov_23.901204, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000102 DAOOMU010000000
VERSION     DAOOMU010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12891_length_6948_cov_23.901204"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            591..2339
                     /locus_tag="PLI55_08755"
     CDS             591..2339
                     /locus_tag="PLI55_08755"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HPB00959.1"
                     /translation="MKGYFSLVLHSHLPYVINHGEWPHGMDWLYEAAAETYLPLLREF
                     NSLIADGISPNITIGITPILAEQLAAERFKDGFINYLEIKIKAARDNREEFATVGNLH
                     LEKLAGMWENYYQGLIDEFNSLPDRNIIQAFKKLQDGNHIEIITCAATHGYLPLLGND
                     EAIDAQVRIGKETYRRHFGCDPRGMWLPECAYRPSYQWSSPIKEYPKTFIRRGIEEFL
                     FKYDIQYFIVDKHLILGGKNRGVYIDRYESLKQLWQQFEKNWTPPPEEKSRSIYQPYL
                     VSSTGTGRVAVAYGRHEESALQVWSGEWGYPGDGRYLEFHKKHFPGGHRYWRVTSAKA
                     DLADKQEYVPENVEEALENQSDHFVEVLRNYILKYYAESGKIGVVTAPFDTELFGHWW
                     FEGPRWLGKVLRKINANPDIQAISLGNHLAKNRPTQVITMPEGSWGQGGFHYIWLNQW
                     TEWTWKHIYAAEDKMVELAEKYRETGDVNLRRILNDLARELLLLESSDWQFLISTWSA
                     RDYAENRVGVHRDQFQNIVRILNDYENNGRLSETDQKYLERMERIDDIFPGLDFTYWS
                     KLPPETGRVDSNNSSK"
     gene            2336..3793
                     /gene="glgA"
                     /locus_tag="PLI55_08760"
     CDS             2336..3793
                     /gene="glgA"
                     /locus_tag="PLI55_08760"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HPB00960.1"
                     /translation="MNIIFIAAEASPFAKVGGLGDVIGALPPVLANRGHSVKVIIPGY
                     RSTWNFKTEPLTQLSNLTVTLGNRVVESQVRRWQNPAQIQHEVYFIDNPEYFGQRNVY
                     CAENGEPYPDNPERFVFFQKAALKLIAETRWSAEIIHCHDNHTALIPVYLKNNLAKVT
                     PFNSLKSVLTLHNIAYQGVCEMRLKEICDLPEELFKPFGALEWYNSLNPLKGGIIFAD
                     AVTTVSPTHAREIMTDEKLSAGMSVVLANRPTPVVGILNGIDTAEWNPATDVHLFAPY
                     SISNLAGKKINKMKLIETLRLNPALKSRPLLGMVSRLVEQKGVDLLIDSLEKILSLGV
                     GFIILGSGEQHYQFELERLANQFRDQMAVKFDYDNILSHRIIAGSDLFLMPSRFEPCG
                     ITQMYALNYGAVPVVHHTGGLVDTVIPWNGKDGNGFAFCEYRSEAMLKTVLEALQVFE
                     KPDEWQKIILNGMSADYSWDKAATKYIELYENLAE"
     gene            3965..5128
                     /locus_tag="PLI55_08765"
     CDS             3965..5128
                     /locus_tag="PLI55_08765"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPB00961.1"
                     /translation="MKTVVMKFGGSSVADTTRLRQVAEIVVRRQQADNRVVVVVSAMG
                     KTTDALIAQAREVSANPQQRELDMLVTAGERISMALLSMAIQELGGDAISFTGSQSGI
                     ITEPTHQGARILEVRPFRLIEALEKGYIVIVAGYQGVSTTKEITTLGRGGSDTTAVAL
                     AAALGADACEIYSDVDGVYTGDPHICPQAQLLREIPYEFMQTMALAGAKVLNADAVEF
                     AKKAGITILARKTGDSSGRETKVHNTAMGSGATAIVLAEKVSQINGNGNLPELLTEIN
                     KAGGRVMALYQRATFDLLVDRTGIPGGDAGRVAQLAKAAGFSANEVAVVTVVGSGLGA
                     RPSLFEKVKVTLSEINIPVLGVFTNDLALSIVIEPTYATGAVRALHQALIVGK"
     gene            5326..>6948
                     /locus_tag="PLI55_08770"
     CDS             5326..>6948
                     /locus_tag="PLI55_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB00962.1"
                     /translation="MKIYLIQPPIEDFYTTPIRNIPQGLLALAANLPKYEINLLDLRS
                     RVRREIVPPPELASVRQFYRPDDASPFGLYKRYYRFGLSEMEIRQTLPRDGDYYLISS
                     LFITYAETVFELIRLIREIVPKSKIIVGGAAATIQPREFLEQGADFVIKGEGELALPR
                     LLNELEKDQPSLAPIPNLVWRNETGVIQENPVNFIEDLNSLPSADYQTPGTPTYYYQK
                     KPHAILITSRGCPHHCSFCCIHQTMGSRYRLRSVENVLNEMAEKIAQGFRSFDFEDDH
                     LGGERDWFLALLKGIAERFTGLNLSLQAMNGITATNLDDEILTAMWRAGFRSLNLALV
                     TPSEAHQRQVGRPFGTEQYLKVVECANKLGFFITTYLIIGLPGDRAEELLESILFLAP
                     LPVLIGPALFYLVPGTASFQQFSRLGLIPSSVLCYRSSFYPVDSKECSRVAAMTLFRI
                     CRILNFMREVIDNEKTPHCPEIESDRIFLEKDLNGRESRYSLGLGLLKLLFERGILYG
                     TGRKEGNFYPIIREICDQKMIERFLSSTWQISG"
BASE COUNT         1917 a         1409 c         1678 g         1944 t
ORIGIN      
        1 ctatggcgag atcggcgatc ctacccaact gactgatgtc tatcggtcag gcgggtttac
       61 gaatttcgat ttgtagtgaa tcgaggacaa aggtatctac aacaggtaat agtttttgaa
      121 caaaagtctt ccgtaaattt ttaatgaatt gatcgagatg tttccgacgc cgattatgtt
      181 gggttcaagc gatcagtgat gaaaaattgg cacgacattc agattggcag tttagcgaac
      241 aggagatttt tctggctacc taaattttaa attcgcttaa taatttcctc gtcggtctca
      301 taatatacta cctgactcaa agtgtttaat cccggttcac ctaaaacagt accaccggct
      361 ctcttaagtg gatataaata tagctgtttt taggcttatg gaatggatag caagtattta
      421 gataatgctt ggaatgctga gataactcat agcggtttgg tttatttctc tcaaactttc
      481 gctgaataaa aagaatctac aaactcgatt agattctttt cccgcagttt gaaatcttgt
      541 ggcaatgtcg tatttttagc cgtttgtcaa ttgataagta aggattaaag atgaaaggtt
      601 atttttcgtt agtattgcat agtcacctgc cctatgttat taatcacggt gaatggccgc
      661 acgggatgga ttggctttat gaagctgcgg ctgagactta tctcccgctt ctccgcgaat
      721 tcaatagcct aattgccgat ggcattagtc caaacataac gatcgggatc acgccgattt
      781 tagccgagca attagccgcg gaaagattca aggacggttt cattaattat ctggaaatca
      841 aaatcaaagc cgcccgggat aaccgagaag aatttgcaac agtcggcaat ctgcatctgg
      901 aaaaattagc tggaatgtgg gaaaattatt accagggctt aattgatgaa tttaattcct
      961 taccggatcg caatattatc caggctttca aaaaactcca ggatggcaat catatcgaaa
     1021 taattacgtg tgccgctacg catggctatc ttcctttatt aggcaatgat gaagccatag
     1081 atgctcaagt tcgaatcggc aaggaaactt accgccgcca tttcggatgt gatccgcgcg
     1141 gaatgtggtt accggaatgc gcctaccgtc cgagttatca atggtcatca cctatcaagg
     1201 aatatcccaa aaccttcatt cgccgaggta tcgaggaatt cctcttcaaa tacgacattc
     1261 agtatttcat tgttgataag catctaattc tgggcggtaa aaaccgaggc gtttacattg
     1321 atcgttatga atcactgaag caactctggc aacaatttga gaagaactgg acgccgccgc
     1381 ccgaggaaaa gagtcgctcg atttaccaac cttatctagt atcttcgact gggacgggac
     1441 gagtagcggt ggcttatggc cgacatgagg aatccgcctt gcaggtctgg agcggcgaat
     1501 ggggctatcc gggcgacggt cgctatctgg aatttcataa gaaacatttc cctggcggac
     1561 atcgctattg gcgagtaacc agcgcaaagg ctgatttagc cgataaacaa gaatatgtgc
     1621 cagaaaatgt agaagaagcc ctcgaaaacc aatctgatca ttttgtcgaa gtacttagaa
     1681 actacatttt gaaatattat gcggagagcg gcaaaatcgg cgttgtaacg gcgccattcg
     1741 atacggagct tttcgggcat tggtggttcg aaggcccacg ctggctcggt aaagtcttgc
     1801 gtaagattaa tgccaatccg gatattcaag caatttcact cggtaatcat ttagccaaaa
     1861 atcgtccaac tcaggtgatt acgatgccgg aaggtagttg gggacagggt ggcttccact
     1921 atatctggct aaatcaatgg accgaatgga cctggaaaca tatttatgcc gccgaagata
     1981 aaatggtcga attagccgag aagtatcgcg aaaccggcga tgtcaatcta cgacgcattt
     2041 tgaatgactt agcccgcgaa ctgctattgc tggaaagttc cgactggcaa tttctgatca
     2101 gcacctggag tgcgcgggat tatgcggaga atcgcgtcgg cgtgcatcgc gaccagttcc
     2161 agaatatcgt tagaatttta aatgattatg aaaacaacgg ccggctttcc gaaactgatc
     2221 aaaaatattt agaacgtatg gaacgcatag atgacatttt cccgggactc gattttactt
     2281 actggagtaa acttccgccg gaaactggac gggttgattc taataatagt tcaaaatgaa
     2341 tattattttt attgccgctg aggcttctcc atttgctaaa gttggtggct tgggcgatgt
     2401 aatcggcgct ttaccgccgg tgctggcaaa tcgtggtcat tcggtcaaag tcataatccc
     2461 gggctatcgc tcgacctgga actttaaaac cgagccgtta acgcaattat cgaatcttac
     2521 agtcacgctg ggtaaccgcg tggtggaaag ccaggtcagg cgctggcaaa atccagccca
     2581 gattcagcac gaggtctatt ttattgataa tccggaatat tttgggcaac gcaatgtcta
     2641 ttgcgctgaa aatggcgagc catatccgga taatccggaa cgttttgtgt ttttccaaaa
     2701 agccgcttta aaattaattg ccgagactcg ctggtcggct gagattattc actgccatga
     2761 taatcatacg gctttgattc cggtttatct taaaaataat ttggcgaaag taacgccttt
     2821 taattcgcta aaaagtgtcc taactctcca taatattgcc tatcaaggtg tttgtgaaat
     2881 gcggttaaag gaaatctgcg atttgccaga agagctattt aagccgtttg gagcattaga
     2941 atggtataat agcctcaatc ccctaaaagg cggaattatt tttgccgacg ctgtcacaac
     3001 cgtcagccca actcacgctc gtgaaattat gacggatgaa aagctaagcg ccggaatgag
     3061 tgtggtttta gccaatcgtc caactccggt ggtgggaatt ttgaacggta ttgatacggc
     3121 ggaatggaac ccggcgacgg acgttcatct ttttgcgcct tattcgatta gcaatttggc
     3181 tgggaaaaaa attaataaaa tgaaattgat tgaaactttg cgtttaaatc cggcgctaaa
     3241 atcacgacca ttgttgggaa tggtttcacg tttggtggaa caaaaaggcg ttgatctttt
     3301 gattgacagc ttagaaaaaa ttctaagttt aggcgtcggc tttatcattc ttggcagtgg
     3361 cgaacagcat tatcagtttg aattagaacg cctggcaaat caatttcgtg accagatggc
     3421 ggtgaaattc gactatgata atatcttatc acaccgaatt atagccggaa gcgatctttt
     3481 tctgatgcct tcccgtttcg agccgtgcgg aataactcaa atgtatgccc tgaattacgg
     3541 cgcagtgccg gtggtgcatc ataccggcgg cttggtggat acggtaattc cctggaatgg
     3601 taaagatggc aatggttttg ctttttgtga atatcgatcg gaagctatgc ttaaaaccgt
     3661 tttggaagcc ttgcaggttt ttgaaaaacc agacgagtgg caaaagataa ttttaaacgg
     3721 tatgagcgcc gattactcgt gggataaagc cgccaccaag tatatagaat tgtatgaaaa
     3781 tctggcggag taagagtgaa atgacattaa aaaggatcag gtgttgaaga aactgataaa
     3841 tgaaaaaaac actgagcctt ttgctatcgt ctaaagaatg ttagctactt ttagcctgtt
     3901 gttgaaaaat gcaattttca gctgaatcat aaattaaagt gaaaggaagc gcgtcgaacc
     3961 gattatgaag accgttgtga tgaaatttgg cggcagttcg gtggcggaca ctacgcgact
     4021 gcggcaggtt gccgaaattg tcgtgcggcg tcaacaagca gataatcggg tggtagtggt
     4081 cgtcagcgct atgggtaaaa caacagatgc cttgatcgcc caggcgcgcg aagtctccgc
     4141 caatccccaa cagcgcgaac tcgatatgct ggttaccgct ggtgagcgca ttagtatggc
     4201 tcttttatca atggcgatcc aggaactggg cggagacgca atctctttca ctggaagtca
     4261 aagtggcatt atcaccgagc cgacccacca gggcgctcga attcttgaag ttagaccgtt
     4321 tcgtctgatc gaagcccttg aaaagggcta tattgtgatc gtcgccggtt atcagggtgt
     4381 atctacaact aaggaaatta cgaccctcgg tagaggtggt tcggatacta cggcggtagc
     4441 attagccgcc gcgctcggtg ctgatgcctg cgaaatttac tcggacgttg atggagttta
     4501 tacaggcgat ccccatatct gtccccaggc acaattattg cgcgaaattc cctacgagtt
     4561 tatgcaaaca atggcgcttg ccggggcaaa ggtgctgaat gccgacgctg ttgaatttgc
     4621 caaaaaggct ggaatcacga ttctggctcg taaaaccggt gacagtagtg gtcgtgagac
     4681 aaaagtgcat aatactgcga tgggttcagg tgccactgcc atcgttttag ccgagaaagt
     4741 ctcgcagatt aatggcaacg ggaatttgcc cgagttactg acggaaatca ataaagctgg
     4801 tggccgggta atggctctat atcagcgcgc aacttttgat ttgctggttg atcgaactgg
     4861 cattccggga ggcgatgccg gtcgtgtggc tcaattggct aaagccgccg gattctcagc
     4921 taatgaagtc gcggtcgtta ccgtagtggg cagtggttta ggagcgcgac cctcactttt
     4981 tgaaaaggtg aaagtgacat tatccgaaat taatatcccc gttctcggag tttttacgaa
     5041 cgatttagct ctgagtattg taattgaacc gacgtatgcc accggagcag ttagggcatt
     5101 gcatcaagcg cttattgtcg gtaaatgaac gccttaattc agtcaatttt gtaaaaatag
     5161 aaacgtaaat aagatcgaca tttatcggtc aatgactttt tatcattaat ctaaatagat
     5221 tggcgataat tactttcttt tgggaattaa ggtgcatcag gcagaatatc aaaaaaataa
     5281 ttgacagcca gaaaaaccat tttatcttag attataagtg gaaatatgaa gatttattta
     5341 attcaaccgc ctattgagga tttttatacg acgccgatac ggaacattcc gcagggttta
     5401 ttagcgctgg cggcgaattt acccaaatat gaaattaatc tgctggattt gcgttcaaga
     5461 gtgcgacgag aaatcgtgcc gccgccggaa ttagcttcgg ttcgacaatt ttatcggccg
     5521 gatgacgctt ccccttttgg cctttataag agatattatc gctttggact gagtgagatg
     5581 gagatcagac aaactctgcc aagagatggc gattactatc taatttccag cctttttata
     5641 acttacgctg aaacggtttt tgaattaatc cggttaatcc gtgaaattgt accgaagtcg
     5701 aaaatcattg taggcggcgc ggcggcgact attcaaccgc gagaattttt agaacaaggc
     5761 gctgattttg tcataaaagg tgaaggcgaa ttggctttac cccgtttgtt gaatgaattg
     5821 gaaaaagacc aaccgtcatt ggcgccaatt ccgaatcttg tctggcggaa tgaaacgggt
     5881 gtaatacaag aaaatccggt gaattttata gaagacctga attctctacc ttctgccgat
     5941 tatcaaacac cgggcactcc gacttattat tatcaaaaga aaccgcacgc tatcctcata
     6001 accagccggg gttgtccgca tcattgctct ttctgttgta tacatcagac aatgggttca
     6061 cgctaccgct tgcgctcggt tgaaaacgtt ctgaatgaaa tggctgagaa aatcgctcaa
     6121 ggattccgtt ctttcgattt tgaagatgat catctcggcg gtgaacgtga ctggtttctg
     6181 gctttgctga agggaatagc ggaacgattt accggtttga atttgtcatt acaagcgatg
     6241 aacgggatca ccgctactaa tctggatgat gaaattttga ctgctatgtg gcgggctggt
     6301 tttcgctcgc ttaatttagc tctcgtgact ccttctgaag cgcatcaaag gcaagtcgga
     6361 cgcccctttg gtaccgagca atacctcaag gtcgtcgaat gtgccaataa attgggattt
     6421 ttcatcacga cttatcttat tatcggattg ccgggtgatc gggcggagga attactggaa
     6481 tctattcttt ttttagcgcc attgccggtt ttgattggtc ctgctttgtt ttatttagtg
     6541 ccaggcacgg cgtcgtttca gcaatttagt agacttggtc taataccttc gtcagttcta
     6601 tgttatcgtt catctttcta tcctgttgat agtaaggaat gtagccgggt agcggctatg
     6661 actcttttcc gaatctgccg gattttgaat tttatgcgtg aggtgataga taacgaaaaa
     6721 acaccgcatt gccctgaaat cgaatcagat cggatttttc tggagaaaga tttgaacggt
     6781 cgtgaaagtc ggtacagcct cggattgggt ttgcttaaat tgctgtttga gcgtggaatt
     6841 ctctacggta ccggtcgcaa agaaggtaat ttttatccaa ttataagaga aatatgtgac
     6901 cagaaaatga tcgaacggtt tctaagtagc acctggcaaa ttagcggt
//
LOCUS       DAOOMU010000103         6771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_13379_length_6771_cov_9.377159, whole genome shotgun sequence.
ACCESSION   DAOOMU010000103 DAOOMU010000000
VERSION     DAOOMU010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13379_length_6771_cov_9.377159"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLI55_08775"
     CDS             <1..398
                     /locus_tag="PLI55_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB00963.1"
                     /translation="FDHIALEFGGDGNNVRLVFDEITIASSFADLTKTSGISRKETVS
                     FEYSLSQNYPNPFNPTTNITYSLKQTGDVRLSIFDILGREVAVLVDGVQNAGQHTITF
                     TGTDLSTGIYLYRLQVGKEVITKKMVLVK"
     gene            740..3973
                     /locus_tag="PLI55_08780"
     CDS             740..3973
                     /locus_tag="PLI55_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB00964.1"
                     /translation="MKKIIRIIIPAFLLATCLVSLLWSGTTGKITGIITDKKTGEPII
                     GANVIVMGTSLGAATDQNGQYTILYVPPGVYNVNISVIGYAKTTLSDVEVHIDQTTRL
                     DVNLEEEAVVGETVVVVAERKIIKRDVATSSVAVSANEIEILPASNVDDVISMQAGIR
                     GNMAIRGSEAEDMLFLVDGITVRDPRNNEPVTKIPLSAVKEISVERGGFNAEYGQVQA
                     GLVNVVTREGDKSNYSGNFQIKYSPPAPKYFRVDGIPDVHDPDSYWMRPYLDDAVCWT
                     GTTQGEPFTDLNGNGKWDTGEDFEDLNSDGSHTFWDQYMRDQYPSFVGWNEISRQLLT
                     DNDPTNDLTPLGAQRVFLYETRKKQVNDMPDYDIDAGFGGPVPLISEKLGNLRFYTSY
                     RRHRDVLLWAQATPDYVDYDWTMQLTSDISETMKLRISSLLGSISTIAENWNYAYYPH
                     WPNEIANGTGGLLMFNMFSDWAYSKTDIGHRSFAIKLTHTLSSKTFYEVSLENFQRDY
                     HTRPIGLRDTTKTTEIVPGYWVDEYPFGYWPYEVVGITPEIKDGIQASLARDYSNASA
                     TTFKADLSSQVNFHNLIKTGVEFVYNDLDLDYGFIQMQTKGQAYASRVQMQNFPIRAA
                     FYIQDKLETDGFTMNAGLRLDYSDSRVEWWDIFSDPFNPYFISSKYKEDLEFDMVKSK
                     PQWQLSPRLGISHPITENSKLFFNYGHFKQMPQYETLFRVQRSSTGSLRAIGDPNLTL
                     AKTISYELGYDHLLFNSVLVQLAAFYKDISDQQNETTYESINNDTYILITSNGYEDIR
                     GFELTLRKSQGRWFTGLINYTYQASSRGYFGRREIYQDPAHQQRYDEETSNLYQERPV
                     PTPYARANLSFYTPDDFGPVVLNHHILGGFMLNLLLTWEQGGWTTYNPKNASGVINNV
                     QYLDYYGGTLRAGKTVTSKRFRIQFLVDVNNLFNRMTLWDTGDQKYRESLHLPKSDAY
                     GNIPGDDKLGDYRKPGIEWQPMKYQEIIQGTIPPALNTNTIPIYYEGSTGTYWEIVDN
                     QWIEVSKSKIDQINADKAYIFNPGASTWWFLNPRTVVFGVRLSFDLD"
     gene            3998..6259
                     /locus_tag="PLI55_08785"
     CDS             3998..6259
                     /locus_tag="PLI55_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00965.1"
                     /translation="MFKTKIKNHLLFLISAVTVVLMFSNTTFAQDQRWIIVGETQCFF
                     MDYGAETERELNFLTWPTQYGDNQHTLRMNGLWLGARNFFDPVENKEKSVKVIGSGPR
                     LPANHYTMIFPQSIKLIGRHSHPVVAVDNQTGTNNLLYDQVDDYDSNLPCDRMVVAEF
                     NTSMGISVTRKVYGFTQPDHDNYFIQEYVFKNTGIYNENGDVHHQDLQDFWVYLCWRP
                     AFAGVTSSGWTDQPAWGAFASEWGSSTLIHTFNVNSPEGMRGFYSYYGPTNDSNHPLT
                     PAEDWGCPDHLETGVLGSAKYLGGVVLFASNPNNHTVDDPSQPATDAYIGSDGVPMET
                     NVSQYDISFMQQRYNIMTEGHLPASQDEDFFNSVGHNGYVEEWTTGNTYRNTYTQGSS
                     SQGWGFGPYNLADGDSIRIVFAIGVTGLSWEKCREVGANWYAYYKSLTNKPDLYMPDG
                     SLASTTNSWGYAAHNAYKRAWCETGRDSILQVLSNAIDNYNKGYNVPQPPPPPESFTV
                     TSGGDRIRLTWSDNAVNSPNFNGYVIYRSEGNVKHYKTVYTKIFECDKASCVHEFDDV
                     TAARGNNYYYYIQSKSDGQENNGKPLYSSLFYTLTSVAAYLRRPAGNFLEHVRVVPNP
                     YDIRARMWQFGEDFQYDRIAFYELPPICKVKVFTERGDLIWEKYHDNGSGDELWDSMT
                     SSRQIIVSGIYILYVEVPQDIYAEQDIVAARDYYNPKTGEKEISKGDVIFQQGDKMYN
                     KGDSVFRKFVVIR"
     gene            6273..>6771
                     /locus_tag="PLI55_08790"
     CDS             6273..>6771
                     /locus_tag="PLI55_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3308 domain-containing protein"
                     /protein_id="HPB00966.1"
                     /translation="MKTIFKSIVIGIVVGCLTIPVELSAKNEKLAQAGFEFLSVVSDA
                     RAAAMADAMTSLQIGSSALFFNPAGMANIEQLIDLTASNNQWITDIQHNTFSLAFSPT
                     HGRYGVIGLSYQNVNYGDFYGTRVNMRVDAGYDDTGVFQLSAHALGLGYAKRLTEWFS
                     VGGQIR"
BASE COUNT         1923 a         1356 c         1560 g         1932 t
ORIGIN      
        1 gatttgatca tattgcattg gaatttggtg gtgatggtaa taatgttaga ttagtttttg
       61 atgaaatcac aattgcatcc tcttttgctg acttaactaa gacaagtgga ataagtcgta
      121 aagagacagt atcatttgaa tattctttgt ctcagaacta tcccaatccg ttcaatccga
      181 cgaccaatat tacttattct ctcaaacaga ctggcgatgt ccgcctgtca atatttgata
      241 ttttgggtag agaagttgcg gtgttagtag atggtgtcca aaatgccgga caacatacga
      301 ttacttttac tggtactgac ttatccacgg gcatttatct gtatagacta caggtcggca
      361 aagaagtaat caccaagaaa atggttttgg taaaatagcg gttttcaatt cggcgaatgt
      421 gaaatttgtt tagttttttc acatttttga tcaatgaggg gtgacaacag gggaaggcgt
      481 tgaaaagcgc cttcccctat cctgttcaat taaatgcaag gaagttgttc tttgacaata
      541 cggctattta ctcgattcaa ttaacgagac gaatattaat cgagcataag aaataattaa
      601 tctgaagcga ccgaaaatat cacaaggtta aaagaattta tgaggttttt taaaaagata
      661 aaatgtgtga tttatgttca aattttgcta aatccatctc aggatttttc cgttttctct
      721 ctgaaaggag gaattgagaa tgaaaaaaat tattaggata ataattcctg cctttttgtt
      781 agccacttgt ctggtgagct tgctttggtc aggaacgacc gggaaaatta ccggtattat
      841 taccgataaa aagaccggag aacccatcat tggagccaat gtcatcgtga tgggaacttc
      901 tctcggagcc gccactgacc aaaatgggca atacacgatt ttgtatgtgc cgccaggtgt
      961 ttataacgtc aatatttccg taatcggata tgcgaaaacg acgcttagtg acgtcgaggt
     1021 acatattgac cagactacta ggctggatgt gaacttggag gaagaagcgg ttgtcggtga
     1081 gacggtagta gtcgtggctg aacgtaaaat aatcaaaaga gatgtggcta ctagtagtgt
     1141 agccgtttca gccaatgaaa ttgaaatttt accggcatcg aatgttgacg atgtcattag
     1201 tatgcaggca ggtatcagag gcaatatggc aattaggggt agtgaagctg aggatatgct
     1261 atttctggta gacggaatta cggtccgcga tccgcgtaat aatgaaccag taaccaaaat
     1321 tccattgagt gcggtgaagg agatttcggt cgagcgggga ggttttaatg ccgagtatgg
     1381 gcaggttcaa gccggattgg tcaatgttgt tactagagaa ggggacaaaa gcaattatag
     1441 tggtaatttt cagataaagt attctccgcc ggcgcctaaa tattttcggg ttgacggcat
     1501 accggacgtc cacgatcccg attcctattg gatgcgtcct tatctcgatg atgctgtctg
     1561 ctggacggga actactcagg gtgagccttt taccgattta aacggcaatg ggaaatggga
     1621 tacaggtgaa gattttgaag atttaaacag tgatggaagt cataccttct gggatcaata
     1681 tatgcgtgat cagtatccgt cgtttgtcgg ctggaacgaa atttctcgcc agctattgac
     1741 tgataatgac cccacaaatg atctgacacc tctgggagct caacgggtgt ttttgtacga
     1801 aacccgtaaa aagcaagtaa atgatatgcc tgattacgat attgatgccg gattcggtgg
     1861 cccggtgcca ctgatttccg agaagctcgg caatctccgg ttttatactt cgtatcgtcg
     1921 tcaccgtgat gttttactat gggcacaggc tactcccgat tatgttgatt atgactggac
     1981 tatgcaacta acttcggata tttcggaaac gatgaaatta cgcatttcaa gcctgctggg
     2041 gagcatctct actattgctg aaaactggaa ttatgcctac taccctcatt ggccgaatga
     2101 aattgccaac ggtaccggcg gcttactaat gtttaatatg ttcagtgact gggcttattc
     2161 taaaactgat atcggtcatc gttccttcgc aataaaatta actcacacct taagttctaa
     2221 aaccttctat gaagtttcac tggaaaattt tcagcgtgac tatcataccc gcccaattgg
     2281 attacgggat acgactaaaa ccaccgaaat agtgcctgga tactgggtcg atgagtaccc
     2341 tttcggttac tggccttatg aagtagtcgg aattactccc gagattaaag acggcattca
     2401 ggcctcattg gctcgtgatt attcgaatgc cagtgctaca actttcaaag ccgatctttc
     2461 cagccaggtt aatttccata atttgattaa aacgggtgtg gaatttgtct ataacgatct
     2521 ggatctcgat tatggtttta ttcagatgca aactaaaggt caagcctacg ccagccgggt
     2581 gcaaatgcaa aatttcccaa tccgagccgc cttttatatc caggataaac tggaaactga
     2641 tggttttaca atgaatgccg gattaaggtt ggactatagc gactcgcgag tcgagtggtg
     2701 ggatattttt tctgacccat ttaatcccta tttcatttca tccaaatata aagaagacct
     2761 cgaatttgat atggtaaaat ccaaaccgca gtggcaatta agtccccggc tgggtatttc
     2821 acatcccata accgaaaatt ccaaattgtt tttcaactac gggcatttta agcaaatgcc
     2881 ccaatacgaa accctgtttc gggtacaacg cagttcaact ggcagtctcc gggcaatcgg
     2941 cgatcccaat ctaaccctag ccaagactat ttcttatgaa cttggttacg accacctgct
     3001 tttcaattcg gttctcgttc aactggctgc gttttataaa gatatcagcg atcagcaaaa
     3061 tgaaacaact tacgaatcta ttaataacga tacctatatt ttaatcacca gtaacggtta
     3121 cgaggatatt cgtggttttg aactgacact ccgtaaaagt caggggcgct ggttcactgg
     3181 tctgataaat tacacttatc aggcttcgtc tcgcggctat tttggacggc gcgaaattta
     3241 tcaggatccg gctcatcaac aacgttatga cgaagaaacc agtaatttat atcaagaacg
     3301 accggtgcca actccctatg cgcgtgccaa cctgagcttc tatactccgg atgatttcgg
     3361 gccggttgtt ctcaaccacc atatcctggg tggttttatg cttaaccttt tattgacttg
     3421 ggagcaaggc ggctggacta cctataatcc caaaaatgca tcgggtgtta tcaataacgt
     3481 ccagtattta gattattatg gtggaactct acgggctggc aagaccgtta ccagtaaacg
     3541 atttaggatc caatttttgg ttgatgttaa caatctattt aatcgtatga cattatggga
     3601 taccggcgac cagaagtatc gtgaatcatt gcatctgccc aagagtgatg cctatggtaa
     3661 tattcctgga gatgataaac tcggtgatta ccgtaaaccg ggaattgagt ggcaaccgat
     3721 gaaataccag gaaatcattc aaggaacaat tccacctgct cttaatacga ataccattcc
     3781 aatttattat gagggtagca ctggaacata ttgggaaata gttgataatc aatggataga
     3841 ggtttccaaa tccaaaattg accagatcaa tgccgataaa gcctatattt ttaaccccgg
     3901 ggcatcaacc tggtggtttc tgaatccgcg gacagtggtt tttggcgtcc ggctttcttt
     3961 tgatttggat taattataaa agaaatgagg aataccaatg tttaaaacga aaataaaaaa
     4021 tcacctctta tttctaatta gtgcggtgac agtcgtttta atgttttcta acacgacttt
     4081 tgctcaagac cagcgctgga ttatcgtcgg tgaaacgcag tgcttcttta tggactatgg
     4141 cgcagaaacc gaacgcgaat taaattttct aacctggcct actcagtatg gtgacaacca
     4201 gcacacgctc cggatgaatg gattgtggct gggagctcgc aatttcttcg atccagtcga
     4261 aaataaggaa aaatcagtga aagtaatcgg atctggtcca agactcccgg cgaatcatta
     4321 cactatgatt tttccgcaga gtatcaaact tattgggcga catagtcacc cggtggttgc
     4381 tgtggataat caaacaggca ctaataatct tctctatgat caggttgatg actatgatag
     4441 taacctgcca tgtgaccgta tggtagttgc cgagttcaat acttcgatgg gtatatcagt
     4501 aaccagaaaa gtttatggtt tcactcagcc ggatcacgat aattatttta ttcaggagta
     4561 tgtatttaaa aatacgggca tatataacga aaatggcgac gtacatcatc aggatctgca
     4621 agatttctgg gtttatttgt gttggaggcc ggcatttgcc ggagtgactt cctctggctg
     4681 gactgatcaa ccggcttggg gcgcttttgc ctcggaatgg ggtagcagta ctcttattca
     4741 tacattcaat gtcaattctc ctgaaggaat gcgaggattt tatagttatt atggtcccac
     4801 taatgattct aatcatccac taactccggc tgaggattgg ggttgtcctg atcatttaga
     4861 gaccggtgtg cttggttcgg ctaagtattt aggaggtgtc gttctatttg catcgaatcc
     4921 gaataatcat acggttgatg accccagcca gccagctacg gatgcctaca ttggcagcga
     4981 tggcgttcca atggagacta atgtgagtca atatgatatc agttttatgc aacaacggta
     5041 taacattatg accgaaggtc atttacctgc tagccaggat gaagactttt tcaattctgt
     5101 tggccacaat ggttatgtgg aggaatggac tactgggaat acttaccgga acacctatac
     5161 ccaaggcagt agcagtcaag gatggggatt tggcccttat aatctggctg atggcgatag
     5221 catccgaatc gtatttgcca tcggagtgac cggattaagc tgggaaaaat gtcgagaagt
     5281 cggggctaac tggtatgcct attataaaag tctaactaac aaaccggatt tgtatatgcc
     5341 cgacggttcg ctagcatcaa ctaccaattc ctggggctac gcggctcata atgcctataa
     5401 acgagcctgg tgcgagaccg gccgcgattc gattctgcaa gtcctaagta atgctataga
     5461 taattacaat aagggataca atgttccgca accgcctccc ccacccgaat catttacggt
     5521 tacttctggt ggagatcgga ttcgcttgac ctggagtgac aatgccgtaa attcgcctaa
     5581 ttttaacgga tatgtcatct atcgctcaga aggcaatgtc aaacattata aaaccgtcta
     5641 tacaaaaata tttgaatgcg ataaagcaag ttgcgtacat gagttcgacg atgtcactgc
     5701 cgctcgcggt aataattact attactacat ccaatctaag agtgacggtc aagagaacaa
     5761 tggcaaaccg ctatacagca gtctctttta tacactgacc agcgtagcgg cttatctgcg
     5821 tcgcccagct ggaaactttt tggaacacgt acgtgttgtc ccgaatccct acgacattcg
     5881 cgctcgaatg tggcaattcg gcgaggattt ccagtatgat cgaattgctt tctatgaatt
     5941 accgcctatc tgcaaggtaa aggtttttac tgaaaggggc gacctaatct gggaaaaata
     6001 tcacgataac ggttccggcg acgaattgtg ggattcaatg acctcttccc gtcagattat
     6061 tgtcagtggt atatatatcc tttacgtgga agtaccgcag gacatctatg ccgaacagga
     6121 tatcgtagcc gcccgtgatt actacaatcc gaaaaccggg gaaaaagaaa ttagcaaggg
     6181 cgatgtgatt ttccagcaag gtgataagat gtacaataag ggcgattcgg tgtttcgcaa
     6241 attcgtggtg attaggtaag gaggggcgga aaatgaaaac aatattcaaa tctatagtta
     6301 ttggaatcgt agttggatgt ttgacaatac ctgtcgaatt atccgccaaa aacgaaaaat
     6361 tggctcaagc tggttttgaa tttctcagtg tggtatccga tgctcgagcg gcggcgatgg
     6421 cggatgcaat gacaagtctt cagatcggtt caagcgctct attctttaat ccggcaggta
     6481 tggctaatat cgagcaatta attgacctaa ctgccagtaa taatcagtgg ataacggata
     6541 ttcaacataa tacttttagt ctggcgttta gtccgactca tggccgatat ggagtgatag
     6601 gcttaagtta ccagaatgtg aattatgggg atttttatgg cacgcgggta aatatgagag
     6661 ttgacgctgg ctatgatgac accggcgtct ttcagctgag tgcccatgcc ctagggctgg
     6721 gttatgcgaa gcgcttgacg gagtggttca gtgtgggcgg tcaaatccgc c
//
LOCUS       DAOOMU010000104         6718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_13534_length_6718_cov_3.556806, whole genome shotgun sequence.
ACCESSION   DAOOMU010000104 DAOOMU010000000
VERSION     DAOOMU010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13534_length_6718_cov_3.556806"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            859..1290
                     /locus_tag="PLI55_08795"
     CDS             859..1290
                     /locus_tag="PLI55_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00967.1"
                     /translation="MSQSVDELLQDAGVRLATARKYAKELGTAGISAEYLTQFEGQIG
                     TLRTENTAYGEAKNRKSELSESQGVQVLASQDVIRKLRAAAQSAFYGDKITQKEFGIG
                     KDVPATVKALSTELPLLKEVAGRYGFFTLSSSTCRLRLFKW"
     gene            1393..4743
                     /locus_tag="PLI55_08800"
     CDS             1393..4743
                     /locus_tag="PLI55_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006560708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPB00968.1"
                     /translation="MVIDSSIRDNRNRGSVADFLLENLKANSKVNIVSAYFTIYAYET
                     LKNSLDGIEKLNFLFGEPKFVKALDPSKTDKKTFKIEDDGITLSNRMRQSQVAKECAE
                     WIQKKVDIRSIKQTNLMHGKLYHIENNGVEVGLMGSSNFTQRGLGVATSSNIELNMVV
                     DSDRDRKDLKNWFFEIWNDETLVEDVKKEVLQYLEQLYLDQPPEFLYFKTLSHIFKEF
                     LEDQTKGGLLAEQHLLTETEIWKILFDFQKDAVKGAINKINKHRGCIIADSVGLGKTF
                     EALAIIKYFELHNERVLVLCPKKLKDNWTVYQAHNASSLNPFLKDRFGYSVLCHTDLS
                     RENGHSGDIDLANFQWGVFDLVVIDESHNFRNNIKGKRDEYGNVVRRSRYERLMQDIL
                     KSGVHTKVLMLSATPVNNTLSDLRNQISFITEDKDNAFAASESMGIPSIKETLRVAQM
                     KFTEWAAKRVKEQRTSRDLLNELDSAFFRLLDELTIARSRKHIHRYYQGEIDKIGGFP
                     KRSKPESIFSEIDLSGRFLSYDKVNDEIEKYALSLFNPSKYILSEWVSHYEEKARTSG
                     FTQFTQRDRESFLIGMMKINFMKRLESSIESFEITIGRTIEKIEDLEKKIDQFQQLKN
                     KPVEFDNSETEIPTDEDEELKDAFEVGKKVTFKLEHLDLDRWLTDLKKDKDQLSVLYN
                     SAVLVSPNKDAKLKDLKSLIEKKVNHPTKNSQGKVNKKVLIFTAFADTAKYLYNEIKN
                     WAENDLNVHIALVTGGSGGCTTTFAPKGFTGRTDYAWILANFSPISKKRNQMKGMPQE
                     GEIDILIATDCISEGQNLQDCDYLINYDIHWNPVRIIQRFGRIDRIGSLNPEVSLVNF
                     WPTKDLEKYIKLKYRVEARMALVDITATAEDNILSTDEIKDLIKDELKYRDKQLMRLK
                     DEILDLEDFDESVNLSEFTLDDFRIELANYIEANKKLLEAAPLGLYSVVPHVIERTTL
                     FDQKIAEIIQPGVIFCLMQKQNAIENQKVNPLQPYFLVYIRDDKVVRFTFTHPKQILE
                     IFRLLCVGQTKPFEDLCRQFDSETQNGNDMQKYTELMKTAVKSIVHTFEKRATGQLFD
                     SRGAVLPPQEAFLNEETEFELITWLVIK"
     gene            4755..>6718
                     /locus_tag="PLI55_08805"
     CDS             4755..>6718
                     /locus_tag="PLI55_08805"
                     /inference="COORDINATES: protein motif:HMM:NF021424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HPB00969.1"
                     /translation="MKTEEREQKERIQNHICRFQTGDFMTNAESLFTTLGYASDRRVK
                     IDKDYLCNKGLNQEKALFSDWREAGLLYQVTGEDLSNQGFLFEIKFDIDRMQSYLFYA
                     IELEPKIYQRGKLAQITREVNKLFPMPVFILFKYGNRLTFSIIDRRQHKCDESKDVLE
                     KVTLIKDISIINPHRAHIEILYDLSYPELLTNFRFSNFEGLHKAWQKTLDTKELNKKF
                     YRELSAWYFYAKQNVLFPDDVEKDADKRNSINLIRLITRLIFVWFVKEKGLVPEELFD
                     EDFLRGILNYSDKTGSTYYKAILQNLFFATLNLEMNKDKPDSRKFVEREDGFHSDQHL
                     QAAYRYVRFFKEPDKALTLFENIPFLNGGLFENLDFEITKDGKREIRRIDCFSDHPQN
                     ETRLKVPDELFFSDLQKVDLSAVYEDNKKRSEEFRGIINIFKRYKFTIEENTPIEEEI
                     ALDPELLGKVFENLLASYNPETHTTARKATGSFYTPREIVNYMVDESLVAYLETQLKE
                     QVPEFKEMETLGELLRDTLSYTEKEHPFTKAEEIDALINAIDNCKILDPACGSGAFPM
                     GILHKLVYLLHKLDPQNEKWMQRQIERVQSAIREAEKVEDTAIRDHLLHVFLPPTETA
                     EDFVDLVTRLERAAAAVGTPLVVEGYGPPV"
BASE COUNT         2065 a         1433 c         1536 g         1684 t
ORIGIN      
        1 gctgaaatct accttatcgg ttttagagga cataatttta tctgtttttt cgatgcctat
       61 cataattaac cggcaaatat agaacaactg gaggttaatt gcaaacagga aacgggggga
      121 attttcaagc ggcgcccata aacgacccgg aaaattcaaa tgcgtccgaa accgacaaat
      181 attttattgc ataccccgcg cctgtgaata aattaaggcc ggtcgggata acgtaacatt
      241 ataaatatga aagtccggtg cgtattttga gtcttttgct ctttgacaag cgagggtctt
      301 ttcgcaaccg ttttttccgg cggacaattt tagcggagcg tttataatcc aatcaacggc
      361 ttcagggttt ttcgcccggg cggctctgtt ttgcattcat ccggaaaata tttggccgcg
      421 ggcggaaata aattggggta aggccgggat aaattgttct taggtggtta caaacgactc
      481 cagggcgttt ccaaattgca aaaagtttgc gcccaacgac aaaagggcgg aaataaaata
      541 gtgtagggtt gcgccaaatt actcacagat ttttcccaat aacaaaaagg tgtaaatatt
      601 ttgcctgcag gcggttccaa acggctaaag ggtggttata aattgctctg ggatggttcc
      661 aaattagtcg cgggtggtaa taaattgtct gcggatagat ttattttgct tacgggtgga
      721 aatcaaccgc tttaaggtgg taaaaaacta tcaacggatg caagttgaca aatgaacaag
      781 acaaaaaaat attctaagaa gttgaaacat cctcccggcc ggaaataacc ggcgggaggg
      841 taaaacaaag gaggacttat gtctcaaagc gtggatgaat tattgcagga cgccggggtc
      901 agattggcca cggcccggaa gtatgcgaag gaattgggaa ccgccggaat cagtgcggaa
      961 tatctgaccc agtttgaagg acaaatcgga accctccgaa cggaaaatac ggcctatggc
     1021 gaggccaaga accggaaatc ggaattgtcc gaaagccagg gtgtccaggt cctggcttcg
     1081 caggacgtca tccgtaagtt gcgggcggcg gcccagtcgg ctttctacgg cgacaagatc
     1141 acgcagaaag agttcggaat cggcaaggac gtcccggcta cggtcaaagc cctttcgacc
     1201 gagctgcccc tgcttaagga ggtcgccggg cggtatggtt tttttactct ttcgtcttct
     1261 acctgccgcc tgcgcctgtt caagtggtga aaatctgctt gtttaatgtt ggaaaataag
     1321 ttacaataag tgcgtactgt gatcattctc ggcaatcttt gaaaaatcat aaaaaaccta
     1381 aggggatttt gaatggttat agactcttcc attcgtgaca atcgcaatcg tggtagcgta
     1441 gcggattttc ttctggaaaa tttaaaagcg aattctaagg tgaatatcgt ctcggcatat
     1501 tttacgatct atgcctatga gacattgaaa aattctctcg atggaatcga aaaactgaac
     1561 tttctattcg gcgaaccaaa attcgtcaag gcactggatc cgagtaaaac cgataaaaag
     1621 acattcaaaa ttgaagatga tggaatcact ctttcgaacc ggatgcgtca aagtcaggtt
     1681 gctaaagagt gcgctgaatg gattcagaaa aaagttgaca tccgctcgat taaacagacc
     1741 aatctgatgc acggtaaact gtaccacatc gaaaataacg gtgtggaagt ggggctgatg
     1801 ggaagttcaa atttcaccca aagagggtta ggtgttgcca cttcctcaaa catcgagtta
     1861 aatatggtcg tcgatagtga cagagaccgg aaggatttaa aaaactggtt ttttgagatt
     1921 tggaatgatg aaactctagt cgaagatgtt aaaaaagaag tactacagta ccttgaacaa
     1981 ctttacttag accaaccgcc cgaatttctc tactttaaaa cgctctccca tatattcaag
     2041 gaattccttg aagaccaaac taaaggcgga ttgctggccg aacagcatct cctaaccgag
     2101 accgaaatct ggaagattct cttcgatttt cagaaagacg ccgtcaaagg cgcaattaac
     2161 aagatcaata agcaccgcgg ttgtattatc gcagatagcg ttggactcgg aaaaacgttc
     2221 gaagcgctgg caattatcaa gtattttgag ttgcataatg agcgggtact ggtgctttgt
     2281 ccgaagaaac taaaggataa ttggacggtt tatcaggcgc acaatgcgag ttctcttaat
     2341 ccgtttctca aagaccggtt cggatattcg gttctttgtc ataccgattt gagcagggaa
     2401 aacggtcact ccggcgacat cgatctggcg aatttccaat ggggtgtttt tgatctcgtc
     2461 gtcattgacg aatcgcataa tttcagaaac aacatcaaag gcaaacgaga cgaatacggg
     2521 aatgtcgttc ggcgtagtcg ctatgaaaga ttgatgcagg atattcttaa atccggtgtc
     2581 catacaaaag tgctgatgct ttccgcaacg cccgtcaaca acacgctgag cgatctccgg
     2641 aatcagattt cattcattac cgaagataaa gataatgcct ttgccgcgtc ggagtctatg
     2701 gggattccca gcatcaagga gactctaaga gtcgcgcaaa tgaaattcac cgaatgggcg
     2761 gcaaaacgtg taaaggaaca aagaacctcc cgcgatttgc taaatgaact agattccgcg
     2821 tttttccgat tattggatga acttacgatt gctcgctccc gtaaacatat ccatcgttac
     2881 taccagggag agatcgacaa aatcggtggt ttcccaaaac gctctaaacc ggaatccatc
     2941 ttttctgaga tagacttatc aggcaggttt ttatcatacg acaaggtcaa tgatgaaata
     3001 gagaaatatg ccctgtctct attcaatcca tcgaaataca ttttgtccga atgggtgtct
     3061 cattatgagg aaaaagcccg aacgtccgga tttacccaat tcacccagcg cgatcgtgaa
     3121 agttttttaa tcgggatgat gaaaatcaat ttcatgaaac ggttagagag ttcgatagaa
     3181 tcgttcgaaa tcaccattgg tcgtaccatc gaaaagatcg aagacctgga aaagaaaatc
     3241 gaccaatttc agcaattaaa aaataagccg gtggaatttg ataactccga aacggaaata
     3301 ccaaccgatg aagatgaaga attgaaagat gcttttgaag ttggtaaaaa agtcacgttc
     3361 aaactcgaac atttagattt ggacagatgg ctgaccgacc tgaaaaaaga taaagatcag
     3421 ttgagcgttc tctacaactc tgccgtcctc gtatcaccga acaaagatgc caaactaaaa
     3481 gatcttaaat ccctaattga gaagaaagtc aaccatccga cgaaaaacag tcagggaaaa
     3541 gtgaacaaga aagtactcat atttaccgct tttgctgaca cagccaaata cctatacaac
     3601 gaaatcaaga attgggcgga aaatgatctc aacgtgcata ttgctttagt gaccggtggt
     3661 tccggcggct gtacgacgac attcgcaccg aaaggattta ccggtagaac cgattatgcc
     3721 tggattctgg cgaacttctc accgatttcc aagaaacgca accagatgaa aggtatgcca
     3781 caggaaggcg aaattgacat cctgattgcg accgattgta tttccgaagg acaaaactta
     3841 caggattgcg attatctgat caattacgat attcactgga atccggtgcg gattattcaa
     3901 cgattcggaa gaattgaccg gatcggttca cttaatcctg aagtctcttt ggtcaatttc
     3961 tggccgacca aagaccttga gaagtatatc aaactcaaat accgtgtcga agcccgcatg
     4021 gcgttagttg atattacggc aaccgccgaa gataatattt tgagcaccga tgaaatcaag
     4081 gatttgatca aagacgaact gaaatatcgc gataaacagt taatgagact caaggacgaa
     4141 atcctcgacc tcgaagattt tgacgaatcg gtcaacctca gcgaatttac tttggatgat
     4201 tttcggattg agttggcaaa ttacatcgaa gccaacaaga aactgttgga ggcagcgccc
     4261 ttaggcttat attcggtggt cccgcatgtt atcgaaagaa ctacattatt tgatcagaaa
     4321 atcgctgaga tcattcagcc aggcgttatc ttttgcctga tgcaaaagca aaacgccatt
     4381 gaaaaccaaa aggtcaatcc gctgcaacct tatttcctcg tctatatccg ggatgataag
     4441 gttgtacgtt ttacgtttac tcaccccaaa caaatattgg aaatcttccg attattatgc
     4501 gtgggtcaaa ctaaaccatt cgaggatttg tgccgccaat tcgactccga aacgcaaaac
     4561 ggcaacgata tgcagaaata tactgagctg atgaaaacgg cggtcaagtc gatcgtgcat
     4621 acctttgaga aacgcgcaac cggccaactt ttcgattcgc gtggagctgt tttaccacca
     4681 caggaggctt tccttaatga agagaccgaa tttgaattga tcacctggtt ggtaattaaa
     4741 taaagggaaa taaattgaaa acggaagaac gggaacaaaa agagcgaatt caaaaccata
     4801 tatgtcgatt tcagaccggc gattttatga ccaatgccga aagtctattt acgaccctgg
     4861 gatatgccag cgaccggcgc gtaaaaattg acaaagatta tctctgcaat aaaggcttaa
     4921 atcaggagaa ggctttattt agcgactggc gggaagccgg tttgctctat caggtcacag
     4981 gcgaagacct gtctaatcag ggatttttat tcgagatcaa gtttgatatt gatcggatgc
     5041 aatcctatct gttttatgca atagaacttg agcctaaaat ttatcagcgt gggaaattgg
     5101 cacaaattac ccgtgaagtg aataaactct ttccgatgcc ggttttcatt ctgtttaagt
     5161 atggaaatcg tctgactttc tcgatcattg accgccgaca acataagtgc gacgaatcca
     5221 aggatgttct ggaaaaagta actctgatca aagacatcag catcatcaat ccgcaccggg
     5281 cgcatatcga aatactttac gatctgtctt acccggaatt actgacgaat ttcaggttta
     5341 gtaattttga aggactgcac aaagcctggc aaaaaacact cgacactaaa gaactcaata
     5401 agaaatttta ccgcgaactt tcggcgtggt atttttatgc caagcagaat gttttatttc
     5461 cagacgacgt cgagaaagat gccgataaac ggaattcgat caatctgatt cgtttgatta
     5521 cccgtctgat ctttgtctgg ttcgtcaaag aaaagggatt ggtcccggaa gaattgttcg
     5581 atgaggattt tctaagaggc attttaaatt attccgacaa aaccggcagc acttactata
     5641 aagcgattct ccagaacctg ttttttgcca ccctgaatct ggaaatgaac aaggacaaac
     5701 cggatagtcg caaattcgtt gagcgcgaag acggctttca cagcgatcag catttgcaag
     5761 cggcgtatcg ctatgtccgc tttttcaagg aaccggataa agcgttaaca ttatttgaaa
     5821 acattccatt cctgaacggc ggcttatttg agaacctcga ttttgaaata acgaaagatg
     5881 gtaagcgcga aattagaagg atcgattgct tttcggatca tccacagaat gagacaagac
     5941 tcaaagtccc ggacgaactc ttcttttcag atttacagaa agtcgatctc agcgcagttt
     6001 atgaggataa taaaaagcgt tctgaggagt tccgtggaat aatcaatatt ttcaaacgct
     6061 acaaattcac cattgaagaa aatacgccta tcgaagaaga aatcgccctc gatcctgaac
     6121 tcttaggtaa agtcttcgaa aatctgttgg caagttacaa tcccgaaacc cataccactg
     6181 cccgaaaagc aaccggatcc ttttacacac cgcgggaaat tgtcaactac atggtggacg
     6241 aatctttagt ggcttacctc gaaacgcagt tgaaagagca agtccccgaa ttcaaagaga
     6301 tggagacgct cggcgaactg ctgcgggaca cattatccta taccgaaaag gagcacccat
     6361 ttactaaagc tgaagaaatt gatgccctga ttaacgctat agataattgt aaaatacttg
     6421 atccggcttg cggctcaggc gccttcccga tgggcatact gcataaactg gtttacctgc
     6481 tccataaact agatccgcag aatgaaaaat ggatgcagcg ccagattgaa cgcgtgcaaa
     6541 gtgccatccg cgaagccgaa aaagtcgagg atactgcgat ccgcgatcac ctgctgcacg
     6601 tcttcctgcc gccgaccgag accgccgagg acttcgtcga cctggtcacc cggctggagc
     6661 gcgccgcggc ggcggtcggc accccgctgg tggtcgaggg ctacggcccg ccggtcga
//
LOCUS       DAOOMU010000105         6536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_14060_length_6536_cov_11.291776, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000105 DAOOMU010000000
VERSION     DAOOMU010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14060_length_6536_cov_11.291776"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="PLI55_08810"
     CDS             <1..204
                     /locus_tag="PLI55_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HPB00970.1"
                     /translation="LTREFIQHQGIFNYEYLQRILNYPPDPRLRWHYNFLWIVLGLAI
                     WERMFIETENYRKHIVVPKEYYQ"
     gene            304..1080
                     /locus_tag="PLI55_08815"
     CDS             304..1080
                     /locus_tag="PLI55_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00971.1"
                     /translation="MASNPFNIEKKIRQKSRLNEYRELLLFARDEGYILTSLIEWHRN
                     YRQNGQRVLILRHDVDIDPEGAAKMYAIEKSLGAHATYYFRWKTVSEALVHKMRRDGF
                     EVSLHYETLATYCKKHHIKDAKKVTDTVMKTCFNLLEEEISRFKKQFGDIQTIASHGD
                     KRNRILGIPNHAILKYGNRADLDIQFEAYDPEITMLFDAYISDSSITNNFNWKYGLTP
                     QEAIRSHLTTICLLTHPEHWNFNFKKNMKTIITDICERLS"
     gene            1089..2282
                     /locus_tag="PLI55_08820"
     CDS             1089..2282
                     /locus_tag="PLI55_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB00972.1"
                     /translation="MILDKEFPPDERVENEALILLEGGHEVYVLCYGYSRSQTKIENY
                     RGIVIQRIPTSKGLIKRLRVFQHPSLNLYKNFWERKVKKFVVHNHIDVLHIHDLYMVP
                     SGIKARRYIGIQVVVDLHENYPAALKAYTWANTLTGRLIIRPQDWKDIEAVYFQNVDR
                     IIVLSQYYRIQLLRKYTFLKDDDITVYPNVPCVKGLLNYKIRRVLDKEDNFTLLYFGV
                     IARRRGIFTVFEALRILIQQGRQKIKLLLIGPIDTNEKKEFNKYLSDNTLKDQIIHYR
                     WKDISEMPSYITDSDVCLSPILKNDQHESGIANKVFQYMLFARPVIVSDCGPQKDLIE
                     TTGCGLVHKSDNAIDLAMKIQYLQDNPELRKTMGEKGRKAVLEIYNTEIQGKQLIELY
                     NSIDI"
     gene            2310..3401
                     /locus_tag="PLI55_08825"
     CDS             2310..3401
                     /locus_tag="PLI55_08825"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: protein motif:HMM:NF019073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0036065 - fucosylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HPB00973.1"
                     /translation="MLTVHIFTRESLYSYKFINFLYKHFDISEHFFIYRSPHKEQYEY
                     PPSFQIKNILYIDSKSKYILKLIPLLLRSQIIYIHFLPIGITPIIWLIFPSLIPKTSW
                     ILWGDDLYWVETRKKGFKAAIYESIRRRVIVKLPRIIAFMEGDYRIAKSRYNTRAIYQ
                     YVVYPLPTDFEYLESIRCNDLVNEEITIQVGNSGDPSNDHIGVLEHLKRFSDRHFKIF
                     CPLSYGGTKEYINSVIQYGQDAFGEKFSPLLEVIQPKDYSKLLLKVNVAIFGSSRQQG
                     LGNILSLLFLGKKVYIRKETTPYEYFKKINVVIFPYSDLENVSFQELAYISPLIAENN
                     YKIIKNHFNEENYVRMWKEVLSIDTNKRK"
     gene            3398..4483
                     /locus_tag="PLI55_08830"
     CDS             3398..4483
                     /locus_tag="PLI55_08830"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003018136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPB00974.1"
                     /translation="MTMINVTKTYLPQKQKYLEYINKIFKSGWLTNNGELVQNLEKRL
                     EDYLGVPHVILTANGTLALQIAYHLLELKGEVITTPFTFIATTSSLVWEKLIPIFADI
                     EPDTFNIDPIQIEKNINNNTSAILGVHVFGNVCNVGKIEEIANSYKLKVIYDAAHAFG
                     VKYYNKSISEFGDVSIFSFHATKIFHTIEGGAIVLKDDDMAKKARLLINFGIPGPDEI
                     ATLGINAKMNEFQAAMGLCVLDDIQDIIEKRHVVYNRYLEAFIDNNKLTLQQIAPHST
                     QNYSYFPVLFESEKILLQIKQILNQAEIFPRRYFYPSLDTLHFIHPKQKMPISRNFSS
                     RILCLPLYEDLAEEIQDQIIKIILMYC"
     gene            4496..5041
                     /locus_tag="PLI55_08835"
     CDS             4496..5041
                     /locus_tag="PLI55_08835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPB00975.1"
                     /translation="MTSFYNEEEIVKLGLKKYGKNVLISRKASFYSPELIQLGSNVRI
                     DDFCILSGNISIGSYVHISAYTALYGKFGICIEDFVTISGRVLVYSQTDDYSGEVMTN
                     PMVNPNLTRVTGGPVVFKKHSIIAAGCIIFPEVTIGEGAAIGAMSLVNKNLEPWSIYV
                     GIPAKYLKPRSKKIIELEHLI"
     gene            5093..>6536
                     /locus_tag="PLI55_08840"
     CDS             5093..>6536
                     /locus_tag="PLI55_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPB00976.1"
                     /translation="MLNYVKSALKNSAIYSIGNVSTKLIGIILVPLYTQHLSLKEYGI
                     LSILEVSSQLLVTIFSANLYTAFFRWYWEKEYSDRQKSIFFTTFLSLIFISGLMVAGI
                     YGFSNRLSVLLFDNSDFNYLIRLMSIAAALQILMDLVSALMRVQEKPVLYTSVNVAKL
                     TISLILTVYFISSLHMGIDGIYKAQIISYVVYFILVSRYLIKNIRVHLEIRVLKEMIR
                     FSLPLMISSVSGILLSIADRFCLKFLGELSYVGIYSLGYKIANTVKVFIISSVNLAIS
                     PVMYRVMDDPGNKRLYSKTMTYFAFGTMICVIGISVFGQEIIKFLAQNEAYWNAYKVI
                     PIISFGLFFEMLKDTSLIGLNITKKTPVIARIIITSSIINIALNLLLIPYFSYIGSAL
                     ASLVTQIIYFLLIYHSAQKYYPIPYEMEKIGKIMLCGMVLIVFGLILSPYALWIRLTG
                     KLLLILSFPFILYLWNFYEPIEIQTIKGAWR"
BASE COUNT         2170 a         1055 c         1182 g         2129 t
ORIGIN      
        1 ctgacgcgtg aattcatcca acatcaggga attttcaatt acgaatacct tcagcggatt
       61 ctgaattacc cgcctgatcc gcgcctgcgc tggcattata atttcctgtg gatcgtttta
      121 ggattggcga tttgggagag gatgtttatc gagacggaaa attatcgtaa gcatatcgtt
      181 gtcccgaaag agtattatca atgagttcca atagccgcaa actgagaatc gggatggtgc
      241 tggacaatcg ttttccaatt atgaaaaacg tggtatatat ttttataaaa tatcattggc
      301 ttaatggcat caaatccatt taatatcgaa aaaaaaatcc gtcagaagtc acgtttgaat
      361 gaataccgtg aactattgtt gtttgcccgc gatgaaggct atattcttac atcattgata
      421 gaatggcatc ggaattatag acaaaatgga cagcgtgtgt taattctgag gcacgatgtg
      481 gatatagacc cggaaggcgc cgcgaaaatg tacgcaatcg aaaagtcgtt aggcgctcac
      541 gcaacttact atttccgatg gaaaactgta agcgaggcac tcgttcataa aatgcggaga
      601 gatggtttcg aagtttcatt gcattatgaa actttagcca catattgtaa aaagcatcac
      661 attaaggatg ccaaaaaagt gactgatacc gtaatgaaaa cctgttttaa tttgctggaa
      721 gaagaaattt ctcgcttcaa aaaacaattt ggcgatattc agacaatcgc atcccatggt
      781 gataagcgta atcgtatatt gggaattcct aaccatgcta tcttaaaata tggaaatcgt
      841 gccgacctgg atatccaatt tgaagcctat gatccagaaa ttactatgtt attcgatgct
      901 tatatttcgg atagttctat aaccaataat tttaattgga aatatgggct tacgccccag
      961 gaggcaattc gttcacactt aactaccata tgtcttttga ctcatccaga gcactggaat
     1021 ttcaatttta aaaagaatat gaaaacaata ataactgaca tttgtgagcg attatcatga
     1081 gaataggaat gattctggat aaggaatttc caccggacga acgagtggaa aatgaagctc
     1141 ttattctatt agaaggtgga catgaagttt atgttttatg ttatggttat tccagaagtc
     1201 aaactaaaat agaaaactac agagggattg taatacaacg aattccaact tcaaaaggtt
     1261 taattaaacg tttaagagta tttcagcatc catctctgaa tctttacaag aacttttggg
     1321 aacgtaaagt aaaaaaattt gtagttcata accatataga tgtactacat atacatgatt
     1381 tgtatatggt tccatctggt attaaagctc gacgctatat tggtattcag gtggtagttg
     1441 atcttcatga gaattatccg gcagctttga aagcatatac ttgggcgaat acattgacgg
     1501 gccgattaat tatcagacca caagattgga aagatatcga agcggtatat tttcaaaatg
     1561 tggatcgaat tatcgtatta agtcaatatt atcgaataca attacttaga aaatacacat
     1621 ttctcaaaga tgacgatatc actgtttatc caaacgtacc ttgtgttaag ggtttactta
     1681 actataaaat tcggagagta cttgataagg aagacaattt taccctcctg tattttgggg
     1741 tgattgctcg tcgtcgcgga atattcacag tttttgaggc attgcggatt ctaatccaac
     1801 aagggcgaca aaaaattaaa ttattgctga taggaccgat tgatacaaat gaaaaaaaag
     1861 aattcaataa gtatttatcc gacaacacgt tgaaagatca gataatccac tatcgttgga
     1921 aagatattag tgaaatgcca tcatatatta ccgacagtga cgtatgtctc tcaccaattt
     1981 taaaaaatga tcagcatgaa tccgggatag caaataaagt atttcagtat atgctctttg
     2041 ctcggcctgt tatagtcagt gattgtggac cacagaaaga ccttatcgaa acaactggtt
     2101 gtggattagt acataaaagt gataatgcta ttgacttggc aatgaaaata caatatttac
     2161 aggataatcc cgaacttcgg aaaacaatgg gcgagaaagg gcgcaaagcc gtattggaaa
     2221 tctataatac cgagatacag ggaaaacaac taatagaatt atataattct attgacattt
     2281 agagaaatat taatgattga aaaattttta tgctgactgt tcatattttt actcgcgaaa
     2341 gtctttatag ttacaaattt attaattttt tatataagca tttcgatata agtgagcatt
     2401 tttttattta tcgctctcct cataaagaac aatatgaata tccaccatca tttcaaatta
     2461 aaaatatttt atacattgat tcgaaaagta aatatatact taaactaatt cctttattat
     2521 tacggtcaca aataatatat atacactttc taccaattgg aattacccca ataatatggt
     2581 taatttttcc ttctttaatt ccgaaaacta gctggatatt atggggtgat gatctatatt
     2641 gggttgagac tagaaaaaaa ggatttaagg cagccattta tgaatctatc cgtcgccgag
     2701 ttattgtaaa attgcctcgc ataattgctt ttatggaagg agattatcgt atcgccaaat
     2761 caagatataa cacaagagcc atttatcaat atgttgttta tccattacca actgactttg
     2821 agtatctaga aagtataaga tgtaatgatt tagtcaatga agagattacc atccaggttg
     2881 ggaattccgg tgatccatct aatgatcaca ttggagttct tgaacaccta aaacgttttt
     2941 cagaccgaca ttttaaaata ttttgccctt tatcctatgg cggaacaaaa gaatatataa
     3001 actcggttat tcaatatggt caagatgcct ttggagaaaa attttcgccg ttattagagg
     3061 ttatccaacc gaaagattat tcaaaattat tactcaaagt aaatgttgca atctttggct
     3121 catcgcgtca gcaaggactt ggcaacatac tttctttact ttttcttggg aaaaaagtat
     3181 atatcagaaa agaaactact ccgtacgaat acttcaaaaa aattaatgtt gtcatttttc
     3241 catattcaga tcttgaaaat gtaagcttcc aggagctggc ttatatctcg ccgctaatag
     3301 ctgaaaataa ttataaaata atcaaaaacc atttcaatga agaaaattat gttagaatgt
     3361 ggaaagaagt tctaagtatt gatactaaca agagaaaata acgatgatta acgtaacgaa
     3421 aacatattta ccccaaaagc aaaagtattt agaatatatt aataagattt ttaaatccgg
     3481 ctggttaacc aacaatgggg aattagtaca gaatctagaa aaacgtttgg aagattactt
     3541 aggcgttcct catgtaattt tgacagctaa tgggacgcta gctttacaaa ttgcgtatca
     3601 cttattagaa ttaaaaggtg aagtaataac cacgcctttt actttcatag ccacgactag
     3661 tagtttagtc tgggaaaaac tgatccctat ttttgctgac attgaaccag atacttttaa
     3721 tattgatcct atacaaatcg agaaaaatat taataataat acatcagcta ttttaggcgt
     3781 tcacgttttt ggcaatgttt gcaatgttgg aaaaatagaa gaaatagcta attcttataa
     3841 actaaaagta atatatgatg cggcacacgc ttttggagta aaatactaca ataagagtat
     3901 tagtgaattc ggggacgttt ctatatttag ttttcatgct acaaagattt ttcatactat
     3961 tgagggggga gcgattgttc ttaaagacga tgacatggca aaaaaggcaa ggctgttaat
     4021 aaattttggt attcccggac cagatgaaat cgccacttta ggaattaatg ctaaaatgaa
     4081 tgagtttcaa gctgcaatgg gattatgtgt tcttgatgac atccaagata ttatcgagaa
     4141 aagacacgtg gtttataacc gttacttgga agcatttatt gataataata agttaaccct
     4201 ccaacaaata gctccacact ctactcaaaa ttactcctat tttccagttt tattcgaatc
     4261 tgaaaaaatc cttttacaaa taaaacaaat tttgaatcag gctgaaattt ttccgcgacg
     4321 ctacttttac ccttctttag atactcttca ttttattcat ccaaagcaaa aaatgccgat
     4381 ttcgagaaat ttttcatcca gaattttgtg tttaccatta tatgaggatt tggctgaaga
     4441 aatccaagat cagattataa aaattatatt aatgtattgc tgaggaagtg caatgattac
     4501 atcattttat aatgaagaag aaatagtaaa attgggactt aaaaagtatg gtaagaatgt
     4561 attaattagc cggaaagcta gtttctactc gcccgaactc atccagttag gcagtaatgt
     4621 taggatagat gatttttgta ttctctcggg gaatatatca atcggttcgt acgttcatat
     4681 ttcggcctat acagctttgt atggaaaatt cggtatttgt attgaagatt tcgttactat
     4741 ttcagggcgt gttttagtat acagccagac tgatgattat agcggtgaag ttatgaccaa
     4801 tccaatggta aatccgaatt tgacgcgagt aaccggaggt cctgtagtat ttaaaaaaca
     4861 ctcgatcata gcggcaggtt gtataatatt tcccgaagtt acaattggag agggtgctgc
     4921 tataggtgca atgagcctag taaataagaa tcttgaacca tggtcaatat atgttggtat
     4981 tccggcgaag tatttaaaac caagatcaaa aaaaataatt gaacttgagc atctgattta
     5041 gaattaattt aatttttagt taattgtgta ctcgttattg taacactttc caatgcttaa
     5101 ttacgtcaaa tcggctttaa agaattctgc tatttacagt atcggtaacg tttccacgaa
     5161 attaattggg attatattag ttccgcttta tacccaacat ctctctctaa aagaatatgg
     5221 aattctgagt attttggaag ttagttcgca actgctagtt acgattttca gtgcgaattt
     5281 atatacagcc ttttttcgat ggtactggga aaaagaatat agcgatcgtc agaagtcaat
     5341 attttttact acatttttat ctctaatttt tatctcagga cttatggtag ctgggatata
     5401 cggtttttcc aatcggttat ctgttttatt gttcgataat tcagatttta attatctaat
     5461 aagattaatg tccattgcgg cggctttaca gattctgatg gacttagttt cagcacttat
     5521 gcgagttcag gaaaaaccgg ttttatatac ttccgtcaat gtcgccaaat taacaattag
     5581 tctaattctg acagtatatt ttatcagcag tttacatatg gggatagatg gaatatataa
     5641 agcccagatt atcagctatg tcgtttattt tattttagta tcaagatatt tgattaaaaa
     5701 tatacgggtt catctggaaa tacgagtttt aaaggaaatg attcgtttta gtctgccctt
     5761 gatgatctct tctgtatcgg gaatattatt aagtattgcc gatcgatttt gtctgaaatt
     5821 cttaggagag ctctcttacg ttggaatcta ctcgctaggt tataagattg ccaataccgt
     5881 caaggttttt atcattagtt cggtaaatct ggctatttcg cccgttatgt atcgagtgat
     5941 ggacgatccc ggtaataaac gtctttattc caagactatg acctatttcg cgtttggaac
     6001 tatgatttgt gttattggga tttccgtctt tggccaggaa attatcaaat tcttggcgca
     6061 aaatgaagcc tactggaatg cgtataaggt aattccgata atttcgtttg gtttgttttt
     6121 cgaaatgttg aaagacactt cgctaattgg attaaatata actaaaaaaa cacccgttat
     6181 cgcacgaata ataataacat cttcgattat caatattgct ctaaatcttt tacttatacc
     6241 atatttcagt tatattggtt cagcgctggc atcactggtt acgcagataa tttatttcct
     6301 tttaatatac cactctgcac aaaaatacta tcccatacct tacgagatgg aaaaaattgg
     6361 caaaataatg ttatgcggaa tggtattaat tgtatttgga ctaatcttat ccccctatgc
     6421 tttgtggatt cggctaactg gaaaattgct attaattcta tcatttccat ttatcttata
     6481 cctctggaat ttctacgagc ctattgagat tcaaaccatc aaaggcgcct ggcgca
//
LOCUS       DAOOMU010000106         6482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_14222_length_6482_cov_16.156994, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000106 DAOOMU010000000
VERSION     DAOOMU010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14222_length_6482_cov_16.156994"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLI55_08845"
     CDS             <1..336
                     /locus_tag="PLI55_08845"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPB00977.1"
                     /translation="QREGLKDPTAHAEMLAITSAATFLDSWQLGDCTLYVTLEPCPMC
                     AGAILNARISRVVFGAYDDAAGMCGSVENLCDQNLLNHKALVRGGVKAEICRSLLNSF
                     FNKIRSTEN"
     gene            349..1671
                     /locus_tag="PLI55_08850"
     CDS             349..1671
                     /locus_tag="PLI55_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009606163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPB00978.1"
                     /translation="MNADLFIIIGGLVIISLLVIIMAKVRKKTDQSGDAALEALKEKI
                     IALESQLNIFPELVQSKTADIVQKRFTEFTDNVSTKNREMIEKFGNFQVEITKSLGET
                     SKKQTEEFSQFKDTFKRAVSEDFEHLTKNVAEKLDAINNKVQENLSEGFKKTNETFTN
                     VIERLAKIDEAQKNIESLSTNVVSLQEILSDKKSRGIFGEVQLNQVLYAIFGEKNDKI
                     FQIQYTLSNDKTVDAILFLPEPIGNIPIDAKFTLDNYKRSIDKSLSEAERIAATKLFK
                     ADVKKQIDEIADKYILPGETSNQAIMFIPAEAVFAELYAYHDDLIEYSHSRRVWLTSP
                     TTFMAVLNTVQLVLRDIERRKYADIIQQEIMKLSEEFRRYRDRWNKLSAHIDTVQKDV
                     KDIHTTSNKISNSFEKIAKVELGEKSELPPEQEVKYLNVINEEESEQE"
     gene            1672..2232
                     /locus_tag="PLI55_08855"
     CDS             1672..2232
                     /locus_tag="PLI55_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006783692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPB00979.1"
                     /translation="MNLLALILIAIGLAMDAFAISITCGLAAQPHNKFKFLKVPLAFG
                     GFQALMPLIGWLAGQTLQRFLANFDHWVAFGLLVFIGIRMIYEAFSKKECTNAIDPLN
                     SHRLFGLSLATSIDALAVGITFAFLEINIVVPIIIIGVITFIISFTGIIIGKRIGHYL
                     GKKVEVVGGIILIAIGFKILIEHLVA"
     gene            2244..2720
                     /locus_tag="PLI55_08860"
     CDS             2244..2720
                     /locus_tag="PLI55_08860"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HPB00980.1"
                     /translation="MIYPACREVKHLLSGEVRTFDCELIFLDEDFGILRYILEKPYVV
                     NNQILPKNTQTYAFYWESQPFTLYRWFAPQGGHLGDYFNIADRIHLSNFQFEWRDLIV
                     DVFISPQGEISLLDENELPADIDGNLARYIDQSTQFIIGNHRKILSELNSLTAKLF"
     gene            2821..4035
                     /gene="serA"
                     /locus_tag="PLI55_08865"
                     /pseudo
     CDS             2821..4035
                     /gene="serA"
                     /locus_tag="PLI55_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020598794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
     gene            4063..6477
                     /gene="leuS"
                     /locus_tag="PLI55_08870"
     CDS             4063..6477
                     /gene="leuS"
                     /locus_tag="PLI55_08870"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPB00981.1"
                     /translation="MYPFKEIELKWQRYWEDNKTFKTPDKSNKPKYYVLDMFPYPSGA
                     GLHVGHPEGYIASDIIARYKRKKGFNVLHPMGWDAFGLPAENYALQTGTHPKITTERN
                     INTFRRQIKSFGFSYDWDREINTTDPKYYKWTQWIFQQIYKMGLAYLAEVPVNWCPEL
                     KTVLANEEVINGKSERGGYPVIRIPMRQWMLKITAYADRLLEDLDLVDFPESIKEMQR
                     NWIGRSEGADIDFKIEDSDQTIRVFTTRPDTLFGATYMVLAPEHPILKTLVPPEYKKT
                     VEAYQQAAASKSDLDRTELAKEKTGVFTGLYAINPANNRRIPIWTSDYVLMSYGTGAI
                     MAVPGHDQRDWEFAKKFGLEIVEVISGGDITKEAYVGDGILVNSGFLNGLNVAEAKRR
                     IIEWLSEKGIGQSRVQYKLRDWIFSRQRYWGEPFPLYYDEDNNIHLIDESKLPLELPP
                     LEKYEPSGTGESPLANLPEWVDFIDPETGKHCRYDTNTMPQWAGSCWYYLRFIDPHND
                     EAFCDREKEKLWMPVDLYIGGAEHAVLHLLYARFWHKVLYDLGYVSTPEPFMKLVNQG
                     MILGEDGEKMSKSRGNVINPDEIIDTYGADTFRMYEMFMGPLTRVKPWNTSSIEGVFR
                     FLNRVWNLYVTEEGLISPDIKDITPDASTNSILHKTIFKITDDIENLRFNTAISQMMI
                     LVNEIAKLAVKPLSVMKTFLHLLNPFAPHISEHIWNLMGNEQSLAYLDWPTYDPSLII
                     DDLVTMAVQVNGKIRGTFDIERDSEDQACIDKALQIDNVQRYLEGHTIVKTVVVKNKI
                     VSIVLK"
BASE COUNT         1990 a         1233 c         1416 g         1843 t
ORIGIN      
        1 cagcgggaag gcttgaaaga cccaaccgcc catgctgaaa tgttggcgat cacctcggcg
       61 gctacttttt tagattcctg gcagttaggg gattgtactt tatatgttac tctagaacct
      121 tgtccgatgt gtgccggggc tatcttaaat gctcgaattt cacgagtcgt tttcggtgcg
      181 tatgatgatg cggctggaat gtgcggttcg gtcgaaaacc tttgcgacca aaacctgctc
      241 aatcataagg ctttggtgag aggcggagtt aaagctgaaa tatgccgatc tttactaaat
      301 tcatttttca ataaaatccg ttcaacggaa aattaaaagg caatagttat gaatgcagac
      361 ttatttatca taatcggtgg cttagttatt atcagcttac tggtaattat aatggcaaaa
      421 gtgagaaaaa agacggatca atccggcgat gccgctctgg aagctttaaa agaaaaaatt
      481 attgccctgg aatcgcagtt gaacattttc ccggaattag ttcagtcgaa aacggctgac
      541 attgtccaaa agcgttttac cgaatttacc gacaacgttt ctaccaaaaa tcgtgaaatg
      601 atcgagaagt ttggcaactt tcaagtagaa atcaccaaat cgctcggtga gacaagcaaa
      661 aagcagactg aagaattcag tcagttcaaa gatactttca aaagggcggt atctgaagat
      721 tttgagcatt tgactaaaaa cgtcgccgaa aaattagatg cgatcaataa taaagttcag
      781 gaaaatctca gtgaaggttt caaaaaaacc aatgaaacat ttacgaatgt gatcgagcgg
      841 ctggcaaaaa ttgatgaagc tcagaaaaat atcgaaagcc tctcgactaa tgtcgtctca
      901 cttcaggaaa ttcttagtga taaaaaaagc cggggcatat ttggtgaggt gcagctcaac
      961 caggtcctct atgctatttt tggcgaaaag aacgataaaa tattccagat tcaatatacg
     1021 cttagcaacg acaaaactgt ggatgcaatc ttgtttttac cggaaccaat cggcaatatt
     1081 ccaattgacg ccaaattcac actggataat tacaaacgct cgattgataa gagcctgtcc
     1141 gaagctgaac gtattgcggc aacaaagttg ttcaaagctg acgtgaagaa gcagattgac
     1201 gagattgccg ataagtacat tttgccgggc gaaacttcca accaggcgat tatgtttatc
     1261 ccagccgaag cagtttttgc cgaattatat gcttatcatg acgatttaat agaatattct
     1321 cattctcgtc gagtttggtt gacttcgcct actactttta tggcggtttt gaatacggtt
     1381 caactcgtct tgcgtgacat agagcgccgc aaatatgccg acattatcca acaggaaatt
     1441 atgaaattat ccgaggagtt ccgtcgttat cgcgaccgct ggaataaatt atccgctcat
     1501 atagacacgg ttcaaaaaga tgtcaaggac attcatacaa cttccaataa aatttccaat
     1561 tcattcgaga aaattgccaa agtcgagctg ggagaaaaat ccgagctgcc gccggaacag
     1621 gaagttaagt atcttaatgt gattaacgaa gaagagtcgg agcaggaata attgaattta
     1681 ttagcattaa tattaattgc aattggtctg gcgatggatg cttttgcgat ttcgattacc
     1741 tgcggtttag cagctcagcc tcataataag ttcaagtttt taaaagtccc tttagccttc
     1801 ggcggttttc aggctttaat gccgctgatc ggctggctgg caggccaaac tctccagaga
     1861 tttctggcga atttcgacca ttgggttgct tttgggctat tggttttcat tggaatacgg
     1921 atgatttatg aggctttctc gaaaaaagaa tgtaccaatg caatcgatcc attgaatagt
     1981 catcgtctat ttggtttatc gctggcaacc agcattgacg ctctggcggt tggaattact
     2041 tttgcttttc tagaaattaa tattgttgta cctatcatca ttattggagt tatcaccttt
     2101 attatttcat ttaccggaat aataattggc aaacgaattg gacattacct tggtaaaaaa
     2161 gtcgaagtag tcggaggaat tatcctgatt gccattggtt ttaaaatttt aattgagcat
     2221 cttgttgcct gaacgaaaat aaaatgattt atcctgcctg tcgcgaagta aaacatttat
     2281 tatccggtga agtccggact tttgactgcg agttgatttt tctggatgaa gatttcggga
     2341 tattaagata catacttgaa aaaccatacg ttgtcaataa tcaaattttg ccgaaaaata
     2401 cgcagactta tgctttctat tgggaatcgc aaccctttac tttatatcgg tggtttgctc
     2461 cgcagggtgg ccatctcggc gattacttta atattgctga ccgaattcac ctgagtaatt
     2521 ttcagtttga atggcgtgac ttaatcgtgg atgtttttat ttcgccgcaa ggtgaaataa
     2581 gcctgctcga tgaaaatgaa ttaccggctg acattgacgg aaatctcgcc agatatattg
     2641 atcaatctac ccaatttatt atcggaaatc acagaaaaat acttagtgag ttaaattctt
     2701 taactgctaa gctattttaa taattaaaaa aatatttaaa attggattca ctcaaacggt
     2761 gaaaaagtat taaattgttt taaatttgag cctgaaaaaa tggataataa tatggatgta
     2821 acatttcaga aaagtaaaac caatgtatta ttactggaaa atatttctcc gacggcacag
     2881 cgtatttttc agaacgaagg ttttccggtt gaatcgctgt ccggaagtat tcctcatgaa
     2941 gttttaaagg agaagatcaa agacatctca gttcttggaa tccgctcgaa gacaattatc
     3001 gatgccgaaa tactggaaaa tgcccaaaat ttgatggcta ttggagcctt ttgcattggt
     3061 actaatcaga tagacctgaa agcctgttcc cagaaaggaa tagctgtttt taatgctccc
     3121 tttagcaata cgcgcagtgt ggtagaattg gctatcggag agatcattat gctatttcgt
     3181 ggcattttcg acaaaagtca gaaacttcac gatgggatct gggataaaaa ttccaaaaac
     3241 tgctacgagg tgcgtgggaa gaaaatcggc atagtcggtt atggaaaaat tggagcgcaa
     3301 ctttcaatat tagccgaaga tatgggaatg caggtttact attatgatat cgtcgaaaaa
     3361 ttagcgctcg gcaatgcaaa aaaatgcaat tctctcgatg aacttttggc tatagccgat
     3421 gtaatttcaa ttcacgtgga tggccgtaaa gaaaattacc atctgatcgg cgccgagcaa
     3481 tttaagaaga tgaagccggg cgttttattt ctcaacctca gtcgtggtca cgttgtggat
     3541 attgaagctt tagctgaaaa ccttcaaagt ggtcaggtca gaggagcggc tattgatgtt
     3601 tttccgactg aaccgttaaa taatgaggaa agattttatt gtcaattgtg ccatttgccg
     3661 aatacaatca ttacaccgca tattggcgga aataccgagg aagcccagga aaatattgct
     3721 caattcgtac cctcgaaaat tattagcttt atcaactcgg gcaatacgat tatgagtgtg
     3781 aattttccca atttgcaatt gccccaattc aatggcgccc accgccttat acacgtccac
     3841 aataatgtcc ctggcatatt gtcccaaatc aatcacattt tagccaggca tggtattaat
     3901 atcgttggac aatatttaaa aactaccgag gatatcggtt acgtgataac cgatattgaa
     3961 aaaaatcacg ataatgaagt gctccttgaa ataatgaata taccaaatac aatcaagttc
     4021 agagttttat actgatattt ccaaatttag aacgagtaat taatgtatcc tttcaaagaa
     4081 attgaactga aatggcagag atattgggaa gataacaaga cttttaaaac ccctgataaa
     4141 tctaataaac caaaatacta cgtgctggat atgtttcctt atccatcggg agccggcctg
     4201 cacgtcgggc atccggaagg ttatatcgct tccgatataa tcgctcgtta taaaaggaaa
     4261 aaaggtttca acgtcttaca tccgatggga tgggacgctt tcggattacc agctgagaat
     4321 tatgcgctcc aaaccggaac tcatccgaaa ataactacgg aaagaaatat caataccttt
     4381 cgacgacaaa ttaaatcatt tggattttcc tatgactggg atcgcgaaat taatacaacc
     4441 gacccaaaat actacaaatg gactcagtgg atttttcagc aaatctacaa aatgggtttg
     4501 gcttacctgg cggaagtgcc ggtcaactgg tgcccggaac tgaaaaccgt tctggctaat
     4561 gaagaagtca taaacggcaa atccgagcgc ggtgggtatc ccgtcattcg gattccgatg
     4621 cggcagtgga tgttgaaaat caccgcttat gctgatcgcc ttctggaaga cctggatctg
     4681 gttgatttcc ccgaaagtat taaggaaatg caacggaact ggatcgggcg gagcgaaggc
     4741 gccgatatag attttaaaat cgaagattcg gatcagacta tcagagtctt cactactcga
     4801 ccggatactt tatttggggc tacttatatg gttctggcgc ccgaacatcc gatcttgaag
     4861 actttagttc caccggaata caagaaaaca gtagaagctt atcaacaagc ggccgcttcc
     4921 aaaagtgacc tggaccgcac cgaattagcg aaagagaaaa ccggcgtatt taccggttta
     4981 tatgcaatta atccagccaa taatcgacga attccgatct ggacttccga ttatgtgtta
     5041 atgtcgtatg gaaccggagc gataatggcg gttcccggac acgatcaacg tgactgggaa
     5101 tttgctaaga aattcggact tgaaattgtc gaggtcatat ccgggggcga tattaccaaa
     5161 gaagcttatg tcggcgacgg catattagtt aattcgggat ttctgaatgg actaaatgtg
     5221 gctgaggcta aacgccgaat tattgagtgg ttaagtgaaa agggaattgg acaatcgcga
     5281 gttcaatata aattgcgtga ttggatattt tcccgtcaac gttactgggg tgagccgttt
     5341 cccttgtatt atgatgagga taacaatatt cacctgattg atgaatcgaa attgccgctg
     5401 gaattgccgc ctctggaaaa gtatgaacca agcggaacag gcgaatctcc gctggcaaat
     5461 ttgccggaat gggttgattt tatcgatcca gaaaccggga aacattgccg ctacgatact
     5521 aacacaatgc cgcaatgggc gggatcgtgc tggtactatc tgcgttttat cgatcctcac
     5581 aatgatgaag cattttgcga cagggaaaaa gaaaagttat ggatgccggt tgatttatat
     5641 atcggtggtg ccgagcacgc cgttttgcat ttgctatacg ctcgtttctg gcataaagta
     5701 ttatatgatt tgggatatgt ctcgacgcct gaacctttta tgaaattagt caatcagggt
     5761 atgattctgg gtgaagatgg cgagaaaatg agcaagtcac gcggtaatgt cattaaccca
     5821 gatgaaatca tagataccta tggcgcggat acttttcgca tgtatgaaat gtttatggga
     5881 ccgctgacac gcgttaagcc ctggaatact tccagcattg aaggtgtatt tcgattttta
     5941 aatcgcgtct ggaatcttta cgtaacggaa gaaggtttaa tcagcccgga tattaaagat
     6001 attacgcccg atgcttcgac taattctatt ctacataaaa ccatattcaa gattacggat
     6061 gacattgaga atctgcgctt taataccgcc atctcccaga tgatgatctt ggtcaatgaa
     6121 atcgccaaat tggcggttaa accgctatcg gtaatgaaga cttttctgca tttattgaat
     6181 ccatttgcgc ctcatattag cgaacatatc tggaatctaa tgggcaatga gcaaagtctg
     6241 gcatatcttg actggccaac ttacgacccg agtttaatta ttgatgatct cgttacaatg
     6301 gcggtgcagg ttaatggtaa aattcgcggc actttcgata ttgaacgcga ttccgaagac
     6361 caggcttgca ttgataaagc tctacagatt gacaatgtcc agcgttacct tgaagggcat
     6421 acaattgtta agacggttgt ggtaaagaat aaaatcgtca gcatcgtcct aaaataaaat
     6481 gg
//
LOCUS       DAOOMU010000107         6355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_14590_length_6355_cov_25.665873, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000107 DAOOMU010000000
VERSION     DAOOMU010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14590_length_6355_cov_25.665873"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="PLI55_08875"
     CDS             complement(<1..95)
                     /locus_tag="PLI55_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HPB00982.1"
                     /translation="MSVKVLIKKDREKSLLRHHPWLFSGAIERIEG"
     gene            complement(105..1988)
                     /gene="selB"
                     /locus_tag="PLI55_08880"
     CDS             complement(105..1988)
                     /gene="selB"
                     /locus_tag="PLI55_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPB00983.1"
                     /translation="MQSLVIGTAGHIDHGKSALVKALTGTDPDRLAEEHLRGMTIDIG
                     FAFLNENIAFIDVPGHERFIKNMVTGASTIDVAMLVIAADDGIMPQTREHFEILKLLG
                     IRHGIVVVNKIDLVQPDWLEMVVEDIRQLVEGSFLENAPLFKTSTVSGAGIAELRRFL
                     IELPNQIPEVHSTGLFRLPVDRVFSIKGFGTVVTGTVLSGEIKVGEEIEIMPSGKRAK
                     IRSVQSHNQPLDSVARGQRAALNLVGVSKEQIERGMIIAPPGAFVKTNLLTVYIESLP
                     SAPPIEYNDQLRLHLGTGEYIVRIRLIGRDRLFPGKQGVAQIVLDQPISAGFRDHFIL
                     RSYSPLVTIGGGIVLENSPEPLRKKDEPIAAELERLVCAGNEEWLKWLIFRQGEKLIT
                     IEKLAQVLTATVAEISPLLAKLVKENVIREREGKIIAAERLAECAEKITATLAEFHRQ
                     NPLLPGLEKATLFGNLHFDETLGDWLTEELTQAGKIRAIGDKLAIAGFTPQLSERQTT
                     RQEQILAEITKSALTPPTLAELAEKLQTDLAEMRQLINLLVSQQEVVLIDRIYPFSMA
                     AVAEAETKLIEFLRGKSGATVSELKEVLGISRKFALPLLNYFDQKGMTARSGDKRRLG
                     SID"
     gene            complement(2000..3418)
                     /gene="selA"
                     /locus_tag="PLI55_08885"
     CDS             complement(2000..3418)
                     /gene="selA"
                     /locus_tag="PLI55_08885"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPB00984.1"
                     /translation="MAGRENYPNELKRLPSVQKVLDRLAQSESPIKPAILTTLVRQEL
                     NELRAAIRTGNFISPKSDDLIEVVAEKVAGRINALTNCCLKKVINATGIVLHTGLGRA
                     PFGTEILPKMTELLSGYVNLEFDLDSGERGERLNLTNEKICLLTGAESSAVVNNNAAA
                     VWLALNSLADSREVIVSRGELIEIGGSFRLPDIMAKSGARLVEVGTTNRTYLQDYQKA
                     RTRKTSAILLAHSSNYRIEGFATKPTASEIITWAHSQNLPVIFDLGSGALFPMENVEL
                     PYEPVVAEVVKQGFDLITFSGDKLLGGPQAGIIVGREKYIRCLRKNPLMRILRCDKTT
                     FFLLDYTLSQYLLDKPFPEIETYRLLTTKPAQLKAQAIQILNAIEPEIIKTLNLTVRE
                     VPTEAGSGAMPTVQIPSVAIVAIPRYISETQLAQKFRSYSPPIVGYCRNGEFRLDLKA
                     VQSDDLPLIQSALQAIGRSLLN"
     gene            complement(3430..5274)
                     /locus_tag="PLI55_08890"
     CDS             complement(3430..5274)
                     /locus_tag="PLI55_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HPB00985.1"
                     /translation="MNIYRRVLKLVKPYWGVLTVSIITSLIYVALNSTSIWLTASFTK
                     VLFPATSDTETSQVMPVVPEKGKGSLNDTLKNYTDRLILRDTPLESLRALCLIIFLTF
                     LLKNVFLYIKNVSVGFVQLRMINDVRNKLYERLHDLSLSYFNRKRSGEITSIIMNDVN
                     AMRNSFTVSFDKLLVEPINILVFMVLLFIISWQLALLAVVILPVSGFIISKIGKSIRR
                     KSLRSSRQIAGIMAILDETLQGMRVVKAFAMEKFEIDRFRKESRKYFNLVFRRKKLRE
                     FSSPINEVFGVFIGVLLLWFGGSLVLTGKGIDAEDFVRFAVMLFAIMNPIKSLTNVSM
                     EMQEGLASAQRVFSLLDEKPDVVEKPNAIEIKGFNDKIVYDNVSFKYETSRTEVLSGV
                     NLEIKKGEIVAIVGHSGAGKSTLVDLLPRFYDPTGGRILIDGHDIRDLTFASLRSLMG
                     IVTQHTILFNDTIFNNIAYGMQDFDPEKVRAAAEAANALEFIEEFPEKFETMIGDKGL
                     RLSGGQRQRLAIARALLKNPPILILDEATSSLDSESEQKVQAAIENLMKNRTVLVIAH
                     RLSTIHNADKIVVLEHGRIVEIGTHNGLLSREDSIYRTFYETQFAKNE"
     gene            complement(5271..5906)
                     /locus_tag="PLI55_08895"
     CDS             complement(5271..5906)
                     /locus_tag="PLI55_08895"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPB00986.1"
                     /translation="MPEIISLAKAADIDCQKVLAILQSGAIIAYPTDTIYGLGVDAFQ
                     PQAVQKLLQLKGRTAGKPISVLYADVPSLLSDFKHLNDYQRRIVKTLLPGKITLILPL
                     SSNSIFPVEISADGSVGVRVISLEPMNRILRQYPHPITTTSVNPANAPPAHSAAEIIS
                     YFGDQISLILKYEIADDSLPSTLVKVGKSELEIIRIGAVTMAEIEEKMKQL"
     gene            complement(5899..>6355)
                     /locus_tag="PLI55_08900"
     CDS             complement(5899..>6355)
                     /locus_tag="PLI55_08900"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPB00987.1"
                     /translation="MIFDARSQSGRNKEQIFYKSKANSPRIWKYLRDENILYLVADQD
                     ARRDGIFVKFFDQWSSSHRGPALFALRQKAPLIAASCLMNTKGKYVIRLKELNTDVPP
                     ENKSDPVAWLTQQIAIYFEEQIRKCPEQYYWFHRRWKTKPPPSFIEQNA"
BASE COUNT         1721 a         1477 c         1422 g         1735 t
ORIGIN      
        1 ccttcaattc tttcaatcgc acctgaaaag agccacggat ggtgacgcag gagcgatttt
       61 tcacgatctt tctttattaa tacctttaca ctcatactat ttccttagtc aatagacccc
      121 agcctgcgtt tatctccgct ccgcgccgtc atcccttttt ggtcgaagta attcaacagt
      181 ggcaaggcaa atttgcgcga tataccgagc acttccttca actcgctgac ggtagcgccg
      241 ctttttccac gtaggaattc tatcaacttt gtttccgcct cggcgactgc cgccatgctg
      301 aacggatata ttctgtcaat taaaactact tcttgttgac tgactaagag gtttatcaat
      361 tgacgcattt cggcgagatc ggtctgtaat ttctcagcca gttcggctaa agtaggcggc
      421 gtcaaagctg attttgtgat ttcagccaga atttgttcct gccgagtagt ttgccgctcg
      481 cttaattgcg gagtaaaacc ggcaatagct aatttatcgc cgatagccct gattttgccc
      541 gcctgagtca attcctcagt caaccaatcg cccagcgttt catcgaaatg caaattgccg
      601 aataatgtgg ctttttccag accgggaagg aggggattct gtcggtgaaa ttcagctaaa
      661 gttgcggtaa ttttttcagc gcattcagcc aatcgttcgg cggcgataat tttgccctcg
      721 cgctctcgaa tgacattttc cttaaccaat ttcgccaata gcggtgaaat ttcagccaca
      781 gtagcggtca gaacttgcgc cagtttttcg attgtaatca gtttttcacc ctggcggaat
      841 attaaccact tcaaccattc ctcatttccg gcgcaaacca accgttcaag ttccgcggca
      901 atcggttcat cctttttgcg cagaggttct ggagaatttt ccagcacaat tccaccgcca
      961 atcgttacca aaggtgaata cgagcggaga ataaaatgat cacgaaatcc ggcgctaatc
     1021 ggctgatcga gaactatttg cgcgactcct tgctttcccg ggaagaggcg atcgcgacca
     1081 atcaatctga tccgcacaat atactcaccg gtgccgagat gcaatcgcaa ctgatcattg
     1141 tattcaatcg gcggcgccga cggcagagat tctatataaa ccgtcagcaa attcgttttc
     1201 acgaaagcgc cgggcggtgc gataatcatt ccgcgttcga tttgttcttt acttactcca
     1261 accagattaa gggccgcacg ttgaccgcgg gcaacggaat caagaggttg attatgcgat
     1321 tgcacactgc gaattttcgc gcgctttccg gacggcataa tctcaatttc ttcgccaact
     1381 ttaatttcac cagagagcac cgtccccgtg acaactgtgc cgaagccttt gatcgaaaaa
     1441 acgcgatcta ccggcaggcg aaacaaaccg gtggaatgca cttcgggaat ttggtttggc
     1501 aattcaatca aaaaccggcg caattccgca attccggcac cgctaaccgt cgaagtttta
     1561 aaaagcggcg cattttccag aaaagaacct tcgactaatt gtcgaatatc ctctacgacc
     1621 atttccagcc aatcgggctg aacgaggtca attttgttca cgacgacgat cccgtgccga
     1681 attcccagca gtttgagaat ttcaaaatgc tcacgggttt gcggcattat gccgtcatcg
     1741 gcggcaatta caagcatagc gacatcaata gtactggcac cagtcaccat atttttgata
     1801 aaacgttcat gccccggcac atcaataaac gcaatatttt catttaaaaa ggcgaagccg
     1861 atgtcaatgg tcataccgcg caggtgttct tctgccagac gatcagggtc ggtaccggta
     1921 agggctttga ctagcgccga tttcccatga tcaatatgtc cggcggtacc gataacgaga
     1981 gattgcattt tctttataac taatttagta aggaacgacc aatcgcctgt aaggcagatt
     2041 gaataagcgg caaatcatca gattgaacgg ctttcaaatc caggcgaaat tctccgttcc
     2101 ggcagtagcc gacaatcggc ggtgagtaac tgcgaaattt ttgggctaat tgggtctcgg
     2161 aaatataacg gggaattgcc acgatggcaa cgctgggaat ttggacagtt ggcatagcac
     2221 cactcccagc ctcagtcggt acttcacgca ccgttaggtt gagcgtttta ataatttccg
     2281 gctcaatagc atttaggatc tggatagctt gggcttttag ctgagcgggc ttagtcgtta
     2341 acaaacgata tgtctcaatt tccggaaacg gtttgtcaag caaatattgg ctaagagtat
     2401 aatcgagcag aaaaaatgtt gttttatcac aacgtaagat gcgcatcagc ggatttttac
     2461 gtaagcacct gatatatttt tcgcgaccga cgattatccc cgcttgtggt ccacccagca
     2521 atttatcacc gctaaaagta atcaaatcga atccttgttt gactacctcg gctacgaccg
     2581 gctcgtaggg caattctaca ttttccatag gaaaaagcgc accgctacca aggtcgaaaa
     2641 tgaccggcag gttttgagaa tgcgcccagg taatgatttc ggatgcggtc ggcttggtgg
     2701 caaagccttc aatgcgataa ttggaggaat gcgccagcaa aatcgcgctg gttttccgcg
     2761 tgcgggcttt ttggtaatcc tggagataag tgcgattggt cgtgccaacc tcaaccagcc
     2821 gagccccact ttttgccatt atatccggta aacgaaaact accgccgatc tcgattaatt
     2881 cgccgcgcga tacaatgacc tcccgactat ctgccagcga attcaaagcc agccagacag
     2941 ccgcggcatt gttattgaca actgctgagg attcggcgcc agtcagcaag caaatttttt
     3001 cattggtaag gttaagccgt tcgccgcgtt cgccggaatc cagatcgaat tccagattta
     3061 cgtatccaga aagtaattca gtcatttttg gtaaaatttc agtcccgaaa ggtgcgcgtc
     3121 ccagtccagt atgcagaacg attccagtag cgttgattac tttttttaaa caacaattag
     3181 tcagcgcatt aatccgaccg gcaacttttt cggcaacgac ttctatcagg tcgtcactct
     3241 tgggggaaat gaaatttcca gtacggatag ccgcgcgcag ttcattgagc tcctggcgga
     3301 cgagtgttgt caggatagct ggtttaattg gcgactccga ttgtgccaga cggtcaagga
     3361 ctttttggac tgacggcaac cgttttagct cgttcggata gttttctcta cccgccaaaa
     3421 gtttcgccct tattcatttt ttgcaaattg ggtttcatag aaagtgcggt aaatgctatc
     3481 ttcgcgactc aatagtccgt tatgtgtgcc gatttcgaca attcgtccgt gctctaaaac
     3541 aacgatttta tcggcattat gaatggtaga aagccgatgg gcgattacca gaacggtacg
     3601 gtttttcatc aaattttcga ttgcggcttg aactttttgc tcagattcag aatcgaggga
     3661 tgaagtggct tcgtcgagaa tcaagattgg tggatttttc agtagagctc gagcaattgc
     3721 caagcgctgg cgttggccgc cggaaagtcg caagccttta tcaccgatca tagtttcaaa
     3781 tttttcggga aattcttcga taaattccaa ggcgttggcg gcttccgccg cggcacgcac
     3841 tttttccgga tcgaaatctt gcataccgta agcgatgttg ttgaaaattg tatcgttaaa
     3901 caaaatcgta tgctgggtaa cgattcccat taatgaacgc aggctggcaa aagtcaagtc
     3961 gcgaatatcg tggccatcaa tcagaatgcg gccacctgtg ggatcataaa agcgcggcag
     4021 aagatcaacc agagtggatt taccggcgcc actatggcca acgatggcga cgatctcgcc
     4081 cttcttgatt tctagattta caccgctgag aacttcagtc cgcgaagttt catatttaaa
     4141 agagacattg tcataaacta tcttgtcgtt aaagccttta atctcgatcg cattcggttt
     4201 ttctactacg tccggttttt cgtcaagcaa ggaaaaaacc cgttgagctg atgccaagcc
     4261 ttcttgcatt tccatcgaaa cgttggtcag acttttgatt ggattcataa tggcaaacag
     4321 cataacggcg aaccggacaa aatcttcggc atcaatacct ttcccagtca aaaccagact
     4381 accaccgaac cagagcaaaa gaacgccaat aaatacgcca aaaacctcat taataggcga
     4441 agagaattca cgtagtttct tgcgtcgaaa aacaaggtta aagtatttac ggctttcttt
     4501 acggaaacga tcaatctcaa atttttccat agcaaaagct ttcacaacgc gcataccttg
     4561 gagagtctca tccagaatcg ccataatgcc ggcaatttga cgggaagatc gcagtgattt
     4621 gcgtcgaata ctcttgccga ttttggaaat aataaaacca ctaaccggca aaatgaccac
     4681 cgccagtaat gccaattgcc aggaaataat gaaaagcaat accataaaaa ccagaatatt
     4741 aatcggctcg accagtagtt tatcaaaact taccgtaaag gaattacgca tagcgttgac
     4801 atcgttcatt attattgagg taatttcgcc gcttcgtttg cggttaaaat atgacagtga
     4861 aaggtcatgc aaacgctcgt aaagtttatt gcggacatca ttaatcatcc gcagttggac
     4921 gaaaccgacc gagacattct tgatatatag aaaaacgttt ttcaatagaa aagttaagaa
     4981 aattatcagg caaagagctc gcaaagattc aagtggcgta tccctcagaa tcaatcggtc
     5041 ggtataattt ttcagggtat cgttgagact tcctttgcct ttttccggaa cgacgggcat
     5101 aacctggctg gtttcggtat cagatgttgc cggaaaaagc actttggtga aagaagccgt
     5161 cagccagatt gaggtactgt tgagagcgac ataaatcaaa gaggtaatta tactgactgt
     5221 cagtaccccc caataaggct taactaattt taaaacacgc cgataaatgt tcataattgt
     5281 ttcatcttct cttctatttc agccatagta acggcaccga tccgaattat ttccaattca
     5341 cttttaccga ctttaaccaa cgttgatggt agtgaatcat ctgcaatttc atactttaaa
     5401 attaatgaaa tttggtcacc aaagtaactg attatttcgg cggcggaatg agccggtggc
     5461 gcgttagctg gattcacgct ggtggtcgtt attgggtgcg ggtattgtcg taaaatgcga
     5521 ttcatcggtt cgagactaat aacccggacg ccgacagagc cgtcagcgga aatttcaaca
     5581 gggaaaatgc tattcgagct gagaggcaaa atcagggtaa ttttccccgg taataaggtt
     5641 ttgacgattc tccgctgata gtcattcagg tgtttaaaat cgctcaatag cgacggaaca
     5701 tcggcatata gcacactaat cggttttcca gcggtgcgac ctttcaactg tagtaatttt
     5761 tgaactgctt gcggttggaa ggcatccaca ccgagaccgt aaattgtatc ggttggataa
     5821 gcgatgatgg cgccgctttg caaaatagca agtacttttt ggcagtcaat atcagccgct
     5881 ttggcaagtg aaattatttc aggcattttg ttcgataaaa gatgggggtg gtttggtttt
     5941 ccagcgacgg tgaaaccagt agtattgctc gggacacttg cgaatttgtt cttcaaaata
     6001 gattgcgatt tgctgagtca accaggcgac cggatcgctc ttattctcgg gcgggacatc
     6061 tgtattcaat tctttcaacc tgattacgta tttgccttta gtattcatca aacaacttgc
     6121 ggcgattagc ggcgcttttt ggcgcaaagc aaataaggct ggaccgcggt gactggatga
     6181 ccattgatcg aaaaatttaa caaatatccc atcacgacgg gcatcttgat cagctactaa
     6241 atagagaata ttttcatcgc gcaggtattt ccagatacgg ggtgaattcg ccttcgattt
     6301 gtaaaatatt tgctctttat tcctgccgct ctgacttctg gcatcaaaaa tcatc
//
LOCUS       DAOOMU010000108         5943 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_15989_length_5943_cov_16.522418, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000108 DAOOMU010000000
VERSION     DAOOMU010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5943
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15989_length_5943_cov_16.522418"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..779)
                     /locus_tag="PLI55_08905"
     CDS             complement(6..779)
                     /locus_tag="PLI55_08905"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPB00988.1"
                     /translation="MIIKMVLVGIFTGIVTGLTGASGVMVVVPLLSILTDFSVHEAIG
                     TSLIVDVIAPLAISYVYFKHKNIDIKSGIWIAVGSIIGAQLGASFAGGISEVGLGGTF
                     GIFMVIMGILIWKKGLNHESLAKVMKKVAKFETQTQRIVTALILGFLVGIMTGVLGAG
                     GGGMILLILIFVLNFPIHIAIGTSALIMAITACSGAIGYALHGNTKPVEGLILGISAA
                     GSGMFSAKFANKANEQTLGKIIGVIFLLLGIIMTILNFR"
     gene            complement(975..2408)
                     /locus_tag="PLI55_08910"
     CDS             complement(975..2408)
                     /locus_tag="PLI55_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPB00989.1"
                     /translation="MANQRKRKVENGLVEGRLSRSIWKLAAPMMIGGALLDLFSIVDL
                     FFVGRLGHIEVAALSIAGTVMAIMMMLVQGIGVGTTALISHFTGEKNYEKSDEVLGQT
                     LILGLIGSAIMLVVSFFLIEPLLKLFGATGEVLTYAADYLRINFNYSIIIFLFSGVNS
                     ALQGSGDVRTPLNALIIGNVLNIILDPLLIMGYGPFPAMGVSGSAVATVITRGIGLIY
                     LFSHVLFGHSTIHLGKRHFKPDLILMRRIVSIGFYASLQVFIREVSFLFLMRLVTSYG
                     DVTLAAYGIGSRIRSFIMVPGFGFASAAAVLVGQNLGAGKPERAKKSAWRAVIYYETI
                     AVPVALIFLIFAPTIVGIFNDHPEVITIGSSFLRYLAVTFPFLAFSLVFSQALNGAGD
                     TRTATIVNAIGQLGFRIPLAYFFARTVGLGYTGVWLGINASDVVQGVGMILVFNAGHW
                     QKVFLKHKKKLAYDLEPREIITELPTN"
     gene            2668..3423
                     /locus_tag="PLI55_08915"
     CDS             2668..3423
                     /locus_tag="PLI55_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPB00990.1"
                     /translation="MTKTILITGTTSGFGKSIAERFAAEGWNLILIGRRSDRLMAISA
                     KLGEKVTVLPITLDIRQRQAVMETLTNLPEKFKAVDVLVNNAGLAAGLEPAQSADLDD
                     WDAMVDTNIKGLMYVTRAILPGMVTRNAGHIINIGSVAGSYPYEGGNVYGATKAFVAQ
                     FSRNLRTDVSGTAIRVTNIKPGNAETEFSLIRFKGNVAKAQAVYQGYQPLTAEDIAEM
                     VFWVTHLPSHVNVNELEVMPVAQSWAGYKIHRQ"
     gene            3482..4537
                     /gene="fni"
                     /locus_tag="PLI55_08920"
     CDS             3482..4537
                     /gene="fni"
                     /locus_tag="PLI55_08920"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HPB00991.1"
                     /translation="MIHKITERKKDHLRIICEENVQYEKVTTGFEQFTFIHNALPEID
                     LREVDCTCEFLGYQLQIPLIICPIGGGESDGKELNKALAKVANKARIALGIGSLRPAL
                     IDKSVLGTYAVVRENAPDIPLIANLGAVQLAEGIDLQTLKLLLNEIGADALSIHLNPL
                     QEALQPEGDPAFRNVSQAIEILKDTLPIPIIVKEVGFGLSSGVIRRLGKMGVKWVDVA
                     GAGGTSWSRIEAKRITDSYRKRLAAEFFEWGIPTVSALLEAVKIKGINVIASGGIETG
                     LQFAKAIALGATLGGGAALFVRTWYAQGAEGLAETVNLFGDTLRHAMFCTGCKTLGTF
                     RGNSEIIKNNSNLSNVK"
     gene            4534..4659
                     /locus_tag="PLI55_08925"
     CDS             4534..4659
                     /locus_tag="PLI55_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00992.1"
                     /translation="MKKLFKLICIILVGLLSQVNAAERFAILYTGSVMGETEPCG"
     gene            4741..5655
                     /locus_tag="PLI55_08930"
     CDS             4741..5655
                     /locus_tag="PLI55_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB00993.1"
                     /translation="MDAGNLFFDRDEIETKQLELKQETARLIVDIYNNLKYDAYNVGG
                     HDFALGLDFLKELQAKARFTFISANVVDTLTGKPVFEPYKIVKVGNKNFGIIGVTSVW
                     NEKYRGVGIADPIQTLNKYLPALRKKADYIIVLAALTAGDENKLGAIKEKIDFILMAG
                     SYRYSRNLEVVDGRFIARCGTTGKYVGIITAEINQPKKPLQDISNISVQLESATNRLK
                     SFKTNAGDKSIEEYYADKPTQLKVIRDLQETQNELLRQKSQIVNPVNYELIELDEKIE
                     DDPAIRKKLNDFERRMNAKGYKITPAGD"
     gene            5707..5779
                     /locus_tag="PLI55_08935"
     tRNA            5707..5779
                     /locus_tag="PLI55_08935"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5740..5742,aa:Ala,seq:ggc)
BASE COUNT         1839 a         1273 c         1169 g         1662 t
ORIGIN      
        1 caacattatc taaaatttaa aatagtcatt attatcccaa gtagtaaaaa tattacccca
       61 atgatcttac ctaaagtctg ttcgttggct ttgttagcga acttagcgct gaacattcca
      121 ctacccgccg cgctaatgcc caatattaat ccctcaactg gctttgtatt accatgtaaa
      181 gcatacccta tcgctccaga acaagccgtt atcgccatta ttagagctga ggtcccaatt
      241 gctatatgaa taggaaaatt tagaacgaat attaaaatta ataatatcat acctccaccc
      301 cctgccccta atactcctgt cataatacca actaaaaatc ctaaaattag agcagtcaca
      361 attctttgag tttgggtttc aaattttgcg acctttttca ttacttttgc caaagactcg
      421 tgatttagtc cttttttcca gataagaatg cccataatca ccataaaaat tccaaatgta
      481 ccgcctaaac ctacctctga tattccaccg gcaaaacttg cgcctaattg ggctccgata
      541 attgacccga cggctatcca tattccggat tttatatcta tatttttatg cttaaaataa
      601 acataagata ttgccaaggg agctataaca tcaactatca aacttgtccc aatagcttca
      661 tggacagaga aatccgttag tatactcaat aatggcacca ccaccatcac acctgatgca
      721 ccggtcaagc cagttactat acctgtgaaa atacctacca agaccatttt gattatcata
      781 ttaacgttct ccctttattg aatcgtatct aattttttat gattttagct tgcgacccat
      841 ttttttataa tgatttagga ttctttcaaa gcaattacaa ttcaaaatat ctgaaaatta
      901 attttcaatt tatctcacca aaattttcat taagctaggt aacgattact gaattctgaa
      961 agttcctatt tgcgctaatt tgtaggcaac tctgtgataa tttctcttgg ttctaaatcg
     1021 taagctaatt tctttttatg ctttaaaaat accttctgcc aatgtccagc attaaagaca
     1081 agtatcattc caacaccttg aacaacatca gaggcattaa tacccagcca gacacctgta
     1141 taacctaatc caaccgtacg agcaaagaaa taagccaaag gtatccgaaa accgagctgg
     1201 ccaatggcgt tcacaatggt agcagttctg gtatcaccgg cgccgttcaa agcttgtgaa
     1261 aagaccagcg aaaatgccaa aaaaggaaaa gtaactgcca aataacgtaa gaaggaactg
     1321 ccaatcgtta tgacttcagg atgatcatta aaaatcccta caatcgtggg cgcaaagatc
     1381 agaaaaatta atgctactgg gaccgcaata gtttcgtaat atataaccgc acgccaagct
     1441 gattttttag cccgctctgg ttttcctgcc ccaagatttt gacccaccag gaccgccgcc
     1501 gcgctggcaa atccaaaacc cggtaccata atgaaagacc tgattcgtga accaattcca
     1561 tacgccgcca gtgtaacatc gccatatgac gtaaccaaac gcataagaaa aagaaatgac
     1621 acttcccgaa taaatacttg aagcgaggcg taaaagccaa tactcacaat tcttcgcatc
     1681 aggattaggt ctggtttaaa atgtcttttt cctaaatgaa tagtagaatg gccaaaaaga
     1741 acgtgactaa aaagatatat gaggccaatt cctctggtta ttaccgttgc tactgcggaa
     1801 cctgatactc ccattgccgg aaatggtcca tatcccataa tcagtaaagg atcaagaata
     1861 atattaagaa catttcctat tatcagggca tttaaaggcg ttctgacatc tccggaaccc
     1921 tgtagtgcac tattcacacc gctaaaaaga aaaataatga tagagtagtt aaaatttatt
     1981 cgcagataat cagcggcata ggttaatact tcacccgtgg caccaaaaag tttcaaaagg
     2041 ggttcaatta gaaaaaacga gactaccagc attatagccg agccgattaa tcctaaaata
     2101 agcgtctgcc ccagaacttc atccgacttc tcataattct tttcaccggt aaaatgcgaa
     2161 attaaagctg ttgtccctac acctataccc tgaactaaca tcatcatgat tgccataact
     2221 gtcccggcaa tagagagagc agctacttct atatgtccca acctgccaac aaaaaataaa
     2281 tctactatcg agaaaaggtc aagcaaagcc ccgccaatca tcatcggagc cgctagtttc
     2341 cagatcgaac gcgaaagtcg gccttctact aaaccatttt caaccttccg cttgcgttga
     2401 tttgccatat tattccttag tgtatcgtaa gagaatggag taagtcaaat atttacacat
     2461 ttttgtagtg tttctgactt atatttttat tttttataat tctaaacatc ttcaaacttt
     2521 ggattttaat tttgccagct gagatttcca atagttttta tgtacatttt tataatttaa
     2581 atttaatttg tattcgcttc tcgatcgcat tttccagttg catttttaaa ttgttactct
     2641 gaaattttcg cctatgaaag gagaaatatg actaaaacaa ttttaatcac aggaaccact
     2701 tccggatttg gaaaaagtat cgccgagcgt ttcgccgcag aaggatggaa tttgattcta
     2761 ataggtcgtc gctccgatcg ccttatggct atttcagcca aactcggcga aaaagtaact
     2821 gtattaccaa ttactctcga tattcgtcag cgtcaggcag ttatggaaac tctcaccaat
     2881 ttaccggaaa aatttaaggc agtggatgtt ctggttaaca atgccggttt agccgccggt
     2941 cttgaacctg ctcaatcggc cgatcttgat gactgggatg cgatggtaga taccaatatc
     3001 aaaggcttaa tgtatgtgac tcgggcgatt ttgcccggta tggttacgag gaatgccggg
     3061 catattatta atattggctc ggtagccggc tcctatcctt atgaaggcgg taatgtctat
     3121 ggcgccacta aagctttcgt agctcagttt agtcgtaatc tccgaacaga tgtcagcgga
     3181 acggcgattc gagttactaa tatcaagccc gggaatgccg aaaccgaatt ttcactgatc
     3241 cggttcaaag gtaacgttgc caaagctcaa gcagtctatc aaggctatca gcctttgacc
     3301 gctgaagaca ttgctgaaat ggtcttctgg gtcactcacc ttccctcaca tgtcaatgtc
     3361 aacgaattag aagttatgcc agttgctcaa tcctgggctg gttacaaaat tcaccggcaa
     3421 taatatttat actgataaac gtaattttga attatactga cgattaaata gatttgccgc
     3481 aatgattcat aaaataaccg agcgtaaaaa agatcatctc cgaattattt gcgaggagaa
     3541 tgttcagtat gaaaaagtaa caaccggttt tgaacaattt acctttattc ataacgcttt
     3601 accagaaata gacctccgtg aggttgattg tacctgcgaa tttctcggat atcaactcca
     3661 aatacccctg ataatttgtc caattggcgg cggcgaaagc gatggtaaag aactcaataa
     3721 agctctcgcg aaagtagcca ataaagctcg tatcgctcta gggattggca gtttgcgacc
     3781 ggctttaatt gataaatctg tattgggaac ttacgcagtc gtgcgtgaga atgcaccgga
     3841 tattccatta attgctaatc tgggcgcagt ccagttggcg gaaggaatag atcttcagac
     3901 attaaaatta ttactgaatg aaataggagc agacgcactc tcaattcacc tcaacccact
     3961 tcaagaagct ctccaaccgg aaggcgatcc tgcattccgt aatgtcagtc aggcaattga
     4021 gattttaaaa gatactttgc caattccaat tattgtaaaa gaagtaggtt ttggactttc
     4081 gagcggggtc attagacgct taggcaaaat gggagttaaa tgggttgacg tggcgggtgc
     4141 cggtggcact tcctggtcaa ggattgaagc caaacgcatt accgattcct atcgaaaacg
     4201 attagccgct gaatttttcg aatggggaat tccaaccgta tcggctttat tggaagccgt
     4261 aaaaatcaaa ggcattaatg tcattgcatc cggaggaatc gaaaccggtc ttcagttcgc
     4321 gaaagccatt gctcttgggg caactctggg cggtggtgcg gctttattcg tacgtacctg
     4381 gtacgcccag ggcgccgaag gattggctga aaccgtgaat ttattcggcg atactttgcg
     4441 gcacgcaatg ttttgcaccg gttgtaaaac tttgggaact ttccgcggta attcagagat
     4501 tatcaaaaac aatagcaatt taagtaatgt aaaatgaaga aattgtttaa gctaatttgc
     4561 ataatactcg tcggcttgct gagccaggta aacgccgccg agcgctttgc aatcctatac
     4621 acaggttcgg ttatgggtga aaccgaaccc tgtgggtgac gaacgcatcg gcttggcggt
     4681 ctggctaggc gtgcagaagt tttcaataaa tatcggacga attacccgaa cagtttgaca
     4741 ttggacgccg gtaacctttt cttcgatcga gacgaaatcg agacaaagca attagaactt
     4801 aagcaagaaa ctgcgcgact gatcgttgat atttataata atttaaaata tgacgcttat
     4861 aatgtcggtg gacatgattt cgccttaggt ctcgactttt taaaagaatt acaagccaaa
     4921 gcccggttta ctttcatctc cgcaaacgtg gtggatacat tgaccggcaa accggttttt
     4981 gaaccatata aaatcgtcaa agtcggtaat aaaaattttg gtataatcgg tgtgacttcg
     5041 gtctggaatg aaaaataccg cggtgtcggg attgccgatc ccattcagac tttaaataaa
     5101 tatttgccgg cactgcgtaa aaaggctgac tatatcatag tattagcggc tttgaccgct
     5161 ggtgacgaaa ataaactcgg agcaattaaa gagaaaatag attttatttt aatggcgggt
     5221 tcctatcgct attcgcgtaa tctggaagtg gttgacggca ggtttatcgc ccgctgtggt
     5281 acaactggga aatacgtagg aatcattaca gctgaaatta atcaacctaa aaagccgttg
     5341 caggatattt ccaatattag cgttcagctg gaatctgcca ccaatcggct caagtctttt
     5401 aaaacaaatg ccggcgataa aagcatcgag gaatattatg ccgacaaacc gactcaactg
     5461 aaagtaattc gcgatctcca agaaactcaa aacgaattat tacgtcaaaa aagtcaaatc
     5521 gttaatccgg tcaattatga attaatcgaa ttggatgaaa aaatagaaga cgatcctgca
     5581 attcgtaaaa aattgaacga ttttgagagg cggatgaacg ccaaaggtta taaaataaca
     5641 ccagcgggcg attaacaagg cttgcattac taaacaattc atagtaattt tacgcgcttt
     5701 ttgtaagggg ctgtagctca gctgggagag cgcttgaatg gcattcaaga ggtcgccggt
     5761 tcgatcccga ccagctccac atcaccaaaa cgtagaacag gctttttagc ctgtttttta
     5821 tttaaaggct gtagttcaat tgcctgcccg actgactaat attaattggt caggcgagcc
     5881 tgcctgactt atcccggtca ggagagggag agcgtcccga ttggtcggga aggtcgccgg
     5941 ttc
//
LOCUS       DAOOMU010000109         5875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_16260_length_5875_cov_23.794845, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000109 DAOOMU010000000
VERSION     DAOOMU010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16260_length_5875_cov_23.794845"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /gene="rpsF"
                     /locus_tag="PLI55_08940"
     CDS             <1..383
                     /gene="rpsF"
                     /locus_tag="PLI55_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPB00994.1"
                     /translation="KTNHINIRSSDLRYYENLFIVNPNIEQEKFTQLIQTVKAEIERL
                     DGNILNLEDWGKRRLAYPIQKHRYGSYVLAQFETENVELVKELESWLRLNQGILAFMT
                     VALDEKPRPKTTQPTDQPVDETIG"
     gene            422..658
                     /gene="rpsR"
                     /locus_tag="PLI55_08945"
     CDS             422..658
                     /gene="rpsR"
                     /locus_tag="PLI55_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPB00995.1"
                     /translation="MVLLSKRKICKFCENPEEKIDYKNYKVLRRFITEQGKIIPSRIT
                     GTCARHQRELTKAIKRARNIALLPYTYDVTQNYK"
     gene            680..1123
                     /gene="rplI"
                     /locus_tag="PLI55_08950"
     CDS             680..1123
                     /gene="rplI"
                     /locus_tag="PLI55_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPB00996.1"
                     /translation="MRVILIKDHENLGKIGDTVEVKDGYARNYLIPQGIALVANSAGM
                     KQMQEQIRLKELRHNKALKKAHELAEKLNTLSLTIPVQVGEEDRVFGAVTSQLIAEQL
                     QEKGYEIDKRNIQLEEPIKALGIFDIPIKIFQDVTAKVKLWVIKA"
     gene            1219..3660
                     /gene="priA"
                     /locus_tag="PLI55_08955"
     CDS             1219..3660
                     /gene="priA"
                     /locus_tag="PLI55_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPB00997.1"
                     /translation="MSAKVQYVDVIFPATLEKSFTYQAPSGYDGTLAPGQRVVAPLKS
                     SSAIGFIINLDPIVPANLKIREIQEIIDPQPIFPPELLTFLKKIADYYLTPFGRVLAA
                     AIPAEYRFIKTRTLKASRLDGAQPTEYGDLFAYIAERREVKYSQLKRQFETIYLQKGL
                     MSLKKEGFIAELVEFKPSKYQGHIEKTVRLLNTDEETLSAIARKAPRQAEILNALQSV
                     GEPLKGRDLRRFSPVALNALARKELIAIDTTDRTLSDLWSEFGERRKTITLTEDQYQV
                     FQAISDSIKSAKYNAFLLEGVTGSGKTEIYLEAIRETLAQGRTALVLVPEITLTTHLA
                     ARFRGEFQDQIAIWHSRLTQAQRSAIWGAVLRGEFPVVIGARSAIFMPLPNLGLIIVD
                     EEQDNSFKQKSPEPRYHARDAALLRGVESQATVVLGSATPSLESQYNAIIGKLNKLAL
                     PRRYSKAPTAVIQVVDMKAEIQETGDVQNPFSRLLTAKIKDRLENGQQVLLLQNRRGY
                     SPVLLCPDCGWVPECRNCDIAMTYHKKSATLICHYCNLHETPPAVCPRCRSSNFLYPG
                     VGTQKVEYYLKTAFPDCRYERLDLDASRIRERAPRVLKQFEEGQIQLLIGTQMIAKGL
                     DFPNVTLVGVINADLGLYLPDFRARERVFQLLYQVSGRAGRGDIQGEIIIQTYNPQDF
                     TIRCALQQNLAGFSNFELNERNPLNYPPFSRMALIQISDLNNQRAATTAAAVADFLNA
                     HRGKIELLGPAVAPLARLLNRYRYQIILKSRREKDPNGRILRQLLSNFLNSREYRDLS
                     RQARIIIDIDPLDLL"
     gene            3660..4547
                     /locus_tag="PLI55_08960"
     CDS             3660..4547
                     /locus_tag="PLI55_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HPB00998.1"
                     /translation="MSRRIFIVLIITYQILMATPPIKVVYPDGYEKLAEFTHTAILNA
                     LPNVPAIWQTTTDTIRIQIVARSADFEGAVGSDLPQWVGAVTLFPQDLMVVRSPDLSR
                     STLREYRKTVVHELIHLLQGQFVPLNLTPVWFNEGLALYYAGEFDLREQIVISKALIR
                     NQLIPLEKLEQILKFSQPQADLAYAESASIIEFMENVYGNGTAAAILARMRNGENFEQ
                     AVLKVTDCEYDDLMNQWQTYVEKKYNWIFLLDIQNILWLIIPLIAFLAYWSIRRRNKV
                     TLEQWNDEEINENSDEQEI"
     gene            4507..5442
                     /locus_tag="PLI55_08965"
     CDS             4507..5442
                     /locus_tag="PLI55_08965"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPB00999.1"
                     /translation="MKKSMKIAMNRKFRLILLGMALVSAASAQALYQGLYQPIDARGW
                     GMGSAMVAQTRNSSGVAYNPAILAVVPNKWQVNYTRYVLDIQSTNVLFVWQAPGKGKL
                     AALGGYLDYGSFSESDDNGNELGEFHVSDLNLRLAYGVPITRKLSAGLAAIFVNSDLA
                     NYNSKALLGSAGLLYYDPVSTFSAGLAYTNFGKMLSGYINDDEKIHSALMAGISKKLE
                     HLPMVIAADFMHYSSGENIIKVGGEFILGQNYFLRWGTSSRRFEINTQQTITNFFSSS
                     SLGGGLVINRLQIDLTWYSLGHTGSVFALSIAQNL"
     gene            5454..5849
                     /locus_tag="PLI55_08970"
     CDS             5454..5849
                     /locus_tag="PLI55_08970"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01000.1"
                     /translation="MRKIILILGGTICVVLVGLGLIGCEGDEANQPSYILDIDPIFGS
                     AKYNCKGCHTGATSPYLDLTTYEGLMAGSDNGKVVIPGDADHSFLYEKISQPIPSRGE
                     RMPLGGPFYLTSGEIRLIEDWINLGAKDN"
BASE COUNT         1631 a         1301 c         1405 g         1538 t
ORIGIN      
        1 aaaaaaccaa tcatattaac ataaggagtt cggacttgag gtactatgaa aatttattta
       61 tcgtaaatcc caatatcgaa caggaaaaat tcacgcaatt gattcagact gtcaaagccg
      121 aaatcgagcg tttagatggt aacattttaa atctggaaga ctggggcaaa cgccgtctgg
      181 cttatccaat ccagaaacat cgttacggct cgtatgtgct ggctcaattt gaaaccgaaa
      241 acgtagaact ggtcaaggag ctggaaagct ggctacgttt gaatcagggc attctggctt
      301 ttatgacagt cgcgctggat gaaaagccgc gtcctaagac tactcagcca accgaccagc
      361 ccgtggacga aactattgga tgaacgaaga ttggatgatt taaaaaagaa aggaaaaaaa
      421 tatggtgtta ttgagcaaac gcaaaatttg caaattttgt gaaaatcccg aggaaaagat
      481 cgactataag aattacaagg tattgagaag gttcataacc gagcagggga aaattattcc
      541 cagccggatc accggcacct gtgcccgcca tcagcgcgaa ttgacgaaag cgattaaacg
      601 agcgcgcaat atcgctttac tgccttatac ctacgacgta acccaaaatt ataaatagta
      661 tcagcaacag gaggtatccg tgcgcgttat tttaatcaaa gatcatgaaa acttgggcaa
      721 gattggggat accgttgaag taaaagacgg ctacgcccgc aattacctca ttccgcaagg
      781 catcgcgttg gttgccaata gcgccggaat gaaacaaatg caggaacaaa tccgcctaaa
      841 ggaactgcgc cataataagg ctcttaagaa agcccacgag ctggcggaaa aactcaacac
      901 cctgtcgcta acgatccccg ttcaggttgg agaagaagat cgcgtcttcg gcgccgtgac
      961 ttcccagctg attgccgaac agttgcagga aaagggctat gaaattgaca agagaaatat
     1021 acaactcgaa gagccgatca aagccctcgg cattttcgat attcccataa aaatcttcca
     1081 ggatgtgacc gccaaggtca aactctgggt aatcaaagcc tgatatattt ttcaaatatt
     1141 tattaatcca aagaccgaat cgttccggtt taatcttgtc ttttttattt cttttggata
     1201 atctttatag cgaaaattat gtctgccaaa gttcaatatg tagatgtcat ttttccggcg
     1261 actctcgaga agagtttcac ctaccaggcg ccgagcggtt atgacggaac acttgctccc
     1321 ggtcagcggg tagtagcgcc gctaaaatcc agctcggcga tcggttttat aattaatctc
     1381 gacccgatcg taccagccaa tttgaagatt cgcgagattc aggaaatcat tgatccccaa
     1441 ccgatttttc ctccggaatt gctgaccttt ttgaaaaaaa tagcggacta ttacctcact
     1501 ccctttggcc gggttttagc cgcagctatt cccgcggaat atcgctttat taagacccgc
     1561 accttaaaag caagtcgcct tgacggtgcg caaccgactg aatatggcga tctttttgct
     1621 tatatcgccg aacggcgtga ggtaaaatac agccagctta aacgccaatt tgagacgatt
     1681 tacctgcaaa aaggactgat gtcgttaaaa aaagaaggtt tcatcgccga gctagtggaa
     1741 tttaaaccgt caaaatacca gggacatatc gaaaagaccg taagattatt gaacactgac
     1801 gaagaaacct tgagtgctat tgcgcgcaaa gcccctcgac aagcggaaat cctgaatgct
     1861 ttacagagcg tcggggagcc actgaaaggc agagatttgc ggcgtttttc accggtagcc
     1921 ctcaatgctt tagcccgcaa agaactaatt gcgattgata caaccgaccg aaccctttct
     1981 gatttatgga gtgaattcgg tgaacggcgc aagacgatta ctcttactga agatcagtat
     2041 caagtttttc aggcaatttc cgattcgatc aaaagcgcta aatataacgc ctttttgctg
     2101 gaaggcgtaa ccggtagcgg caagaccgaa atctacctgg aggcaattcg agaaacctta
     2161 gctcaagggc gcacggcgct cgttttggtg ccagaaataa ctttgacaac gcaccttgcc
     2221 gcccgttttc gtggggaatt tcaggatcag attgccatct ggcatagccg actgacccaa
     2281 gcccagcgca gtgctatctg gggtgcggtt ttgcgcggtg aatttccggt tgtcattggc
     2341 gcgcgcagtg ccatttttat gcctctgccc aatctcggat tgattatcgt cgatgaagag
     2401 caggataatt cgtttaagca aaaaagtccc gaaccgcgct atcatgcccg agatgcggct
     2461 ttgttgcgcg gtgtcgaaag ccaggcgacc gtcgtgctag gttcagccac tcccagcctc
     2521 gaaagtcagt acaatgccat tatcggtaaa ttgaataagc tggctttgcc ccgccgttat
     2581 tccaaggctc cgactgctgt aatccaggta gttgatatga aagcggaaat tcaagaaacc
     2641 ggtgatgttc agaatccatt ttcacgtttg ttgactgcca agattaaaga ccgactggaa
     2701 aatggtcagc aggtgcttct cctccagaat cggcggggtt actcgccggt cttgttatgt
     2761 ccggactgcg gctgggttcc ggaatgtcgc aattgcgata tcgctatgac ctatcataaa
     2821 aaatccgcga cgttaatttg ccactactgt aatttacatg aaacaccacc ggcggtttgt
     2881 ccgcgctgta gaagtagtaa ttttctctac ccgggagtag ggactcaaaa ggttgaatat
     2941 tatttaaaga cggcatttcc tgattgccgt tatgaacggt tggatttgga tgctagtcga
     3001 attagggagc gagcgccccg tgttttaaag caattcgagg aaggccaaat tcagctttta
     3061 atcggaactc agatgattgc taaagggctg gattttccca atgtgactct ggtaggagtc
     3121 attaatgccg atcttggtct ttacctacca gatttccggg cgcgcgaaag agtttttcaa
     3181 ttgctttatc aagtttcggg gcgcgccggg cggggtgata ttcagggtga aataataatc
     3241 cagacctaca atccacagga ttttacgatt cggtgtgcgc ttcagcagaa tttagccggt
     3301 ttttccaatt ttgaacttaa tgaacgcaat ccgctcaatt atccgccttt tagccgaatg
     3361 gcgctcatcc agatttccga tttgaataat caacgcgccg ccacgaccgc ggccgcggtt
     3421 gccgatttcc tgaatgccca tcgtggtaaa attgaattac taggaccggc ggtggcgcct
     3481 ttagctcgtt tgttaaatcg ttaccgctat caaataatct tgaaatcacg acgtgaaaaa
     3541 gaccctaatg ggagaatcct gcgccagctg ttgagcaatt ttttgaattc ccgggagtat
     3601 cgagacctga gtcgtcaagc acggatcata attgatatag atccactcga tctactataa
     3661 tgtcgcgccg catttttatc gtattgataa ttacttacca aatccttatg gctacgcctc
     3721 cgataaaagt tgtctatccg gacggttatg aaaaactggc tgaatttacc cacactgcga
     3781 ttttgaatgc tttgccgaat gtgcctgcca tctggcagac gacgactgat acgattcgca
     3841 tccagatcgt ggcgcgctca gccgatttcg agggagctgt cggtagcgat ttaccccagt
     3901 gggttggggc agtcactctc ttcccacagg acttgatggt agtccgatcg cccgacctga
     3961 gtcgtagtac tttgcgggaa tatcgcaaga ctgtcgtcca cgaacttatt catttattgc
     4021 agggacaatt tgtgccgttg aatctgacgc cggtctggtt taatgaaggt ctggcgcttt
     4081 attatgccgg agagttcgac ttacgggagc agattgttat atccaaagcc ttaatccgaa
     4141 accagttgat tccactggaa aaattagaac aaattttgaa attcagtcaa ccgcaggctg
     4201 atttagctta cgccgaaagc gcttcgataa ttgaatttat ggaaaatgtt tacgggaatg
     4261 gaacagccgc ggcaattttg gcaagaatgc gtaacggtga aaatttcgaa caagctgtcc
     4321 taaaagtaac cgattgcgaa tatgacgatt tgatgaacca gtggcaaact tatgttgaaa
     4381 aaaagtacaa ttggatattt cttctggaca ttcagaatat tttgtggtta attataccgt
     4441 tgatagcgtt tttggcttat tggagtattc ggcggcggaa caaagtgact ctcgagcagt
     4501 ggaatgatga agaaatcaat gaaaatagcg atgaacagga aatttaggtt aatattactc
     4561 ggaatggcat tagtgagcgc cgcgtcggcg caagcccttt atcagggact ttatcagccg
     4621 attgatgccc gcggctgggg tatgggttca gcaatggttg cccagacgcg caattcgtcc
     4681 ggcgtggcat ataatccggc gattctagcg gttgttccga acaaatggca ggtgaattat
     4741 acccgctacg tattggacat tcaatcaacc aacgttctat ttgtctggca ggcgccggga
     4801 aaaggtaaat tggcggcatt gggcggctat ctggactacg gcagttttag cgagagtgat
     4861 gataatggaa atgaactcgg cgaatttcac gtcagcgatt taaatctgcg actagcttat
     4921 ggagtcccaa tcacccgcaa actcagcgcc gggctggcgg caatcttcgt gaattccgac
     4981 ttagcaaatt acaattcaaa ggcgttatta ggttcagcgg gtttattgta ttatgatccg
     5041 gtctcgacgt tttcagctgg actggcttat acgaattttg ggaaaatgct ttcaggatat
     5101 atcaacgatg atgagaaaat tcattccgcg ctaatggctg gcatttccaa gaagcttgaa
     5161 catttgccaa tggtgatcgc ggctgacttt atgcattatt catctggaga aaatatcatc
     5221 aaagtcggcg gagaattcat tctcggacaa aattatttct tgcgctgggg gacatcgagc
     5281 cggcgttttg aaatcaacac ccagcagacc atcactaatt ttttcagttc ttcttcgtta
     5341 ggcggcggct tggtaattaa ccgtttacag atcgacctga cctggtacag tctcggacac
     5401 accggcagtg tttttgccct cagcattgcc cagaacctat agaaaggtgc attatgcgaa
     5461 aaataatact aattttaggc gggacaatat gcgtcgtctt agtggggctt ggactaatcg
     5521 gatgtgaagg tgatgaagcc aatcaaccca gctacatttt agacattgat cccatcttcg
     5581 gaagcgctaa atataactgt aaaggatgcc acaccggtgc tacttcgcca taccttgatc
     5641 tgacgaccta cgaaggttta atggccggca gtgataatgg caaggtggtg ataccgggtg
     5701 acgctgacca tagtttttta tatgaaaaaa tttcccagcc aatcccttcc agaggcgaac
     5761 gtatgccgct cggcggtcct ttttatttaa cctcggggga aatcaggtta atcgaagatt
     5821 ggatcaacct gggcgctaag gataattaag ccgagttaga tagattagcc gaata
//
LOCUS       DAOOMU010000110         5594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_17384_length_5594_cov_19.860444, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000110 DAOOMU010000000
VERSION     DAOOMU010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17384_length_5594_cov_19.860444"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLI55_08975"
     CDS             <1..427
                     /locus_tag="PLI55_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01001.1"
                     /translation="VDVLPAKKGDDIFYQSQPVDKKASGFAASLSFRDSDGDGFLMPG
                     EEAALQIDLKPLYAEKRDSVRLQLLCSAESREVSYDHELIFKDCQSNQWNQMIVPIKA
                     LPKLQNRSIVFWGIIETKYGFQILPASLIINGKNPEKNI"
     gene            588..>1303
                     /locus_tag="PLI55_08980"
     CDS             588..>1303
                     /locus_tag="PLI55_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01002.1"
                     /translation="MKANALTQIILMTAALIISSCYTQFSAPIVRQETYQQSVTDTTY
                     QEQETYENYTFDSSYLNDYRLGYRDGYWDSMNSQLWLGFNIYSPFYGGYRYHSPYLGY
                     YDPWFYDPFYDWGFYGYFSFYGGYYPYYGYYPHYGYYGYYYGGYPYHRHHWHGYRDGT
                     YYNEGRDELKADYVPKSTPSGSRGSSNDAGSQSGASYLPTSTSAITTTSVSGEKTTVT
                     TTNSGSTKARYSPKTTGAGN"
     assembly_gap    1304..1403
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1544..2869
                     /locus_tag="PLI55_08985"
     CDS             1544..2869
                     /locus_tag="PLI55_08985"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HPB01003.1"
                     /translation="MSKFCRYFFILAMLVFFLPMSISAQHFDQVMSSEALRPFWSIYG
                     IGASSFNLLNNSHLETDIGSTQSNPAFLTNVKRPKGSFSGISITPKNLTTLANVGKDY
                     SASTTKYNVSYIGFAYPFPVYQGSFVMAASYSPAAYYYSSSYTKGTTGATYYDGDGNA
                     HIIDTDLEYDINESGTLNVFRFAGATEFMKNFNLGLSFNFYNGQRQYESTEIGVDVND
                     ELDFNSIIYQENIKPSYSGFNMDFGICYQSENYKIGLRLSTPLKLKVHEVSELTETYS
                     YDTVDSIFPNSYDFKYRTRYPLEIAPNFAIKVGKISLGMDFIFHNWQKIEVDLLDEMR
                     EVNRDLYWNLRNTTDIGAVLTIPFGNFVSTRLAYRLVPSPYAEYEPDEEYYHVMGAGI
                     ETILKESIIIGCSFQHGFGNLSNFYRYFETTTTQAYTENRCSISLAVLF"
     gene            2985..3335
                     /locus_tag="PLI55_08990"
     CDS             2985..3335
                     /locus_tag="PLI55_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01004.1"
                     /translation="MQFSIAGEIPHIYHIPPKTLIQNNPAEITCVITPGDSQLKWVKI
                     FLRDDSSELYKEFNMQYRDGIWFLPLIPEMVSGNYLDYFIIAEFEDFTALAFPPERPA
                     EKPIRVQIVSTKKI"
     gene            3399..4715
                     /locus_tag="PLI55_08995"
     CDS             3399..4715
                     /locus_tag="PLI55_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HPB01005.1"
                     /translation="MRSRFYLPKIFLILLVGSILLTATPKEIWVLEVDGVINPVSASY
                     INDNLRKAQPDQVECLIIQMDTPGGLMTSMRDIIKNILNSEIPVVVYVSPRGAQCASA
                     GVFIAVSAHFVAMSPGTNIGAAHPVNLGGGGIGGQPDSASSQTMIEKATNDAVAYIRS
                     LARERGRNEEWLEKAVRESASITETEAVKLQVVDVVVDDLDELLEILDGKTFKLPSGE
                     KTIHTQDATIRFRPVGIHHRILDVISDPTVAYILLMLGFYGIYFELSNPGAIFPGVLG
                     AIFLILAFFAFQVLPINYAGLALIILGIILFILEVKIISYGLLTIGGIVAMTLGSLML
                     IDVNQAPEILEAVSLKVILPIVIFTAAFVIISLTLALKAHKAKPTTGIEGMIGETGQT
                     ITDVDKTGMAIVHGEYWKVSSDTPIPKGTEIVVLDVDRMVLKVKPK"
     gene            4744..5508
                     /locus_tag="PLI55_09000"
     CDS             4744..5508
                     /locus_tag="PLI55_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPB01006.1"
                     /translation="MSIPLITIIILLIFLISSAIRVLREYERGVIFRLGRLIAAKGPG
                     LFFLIPVVDRMVKVSLRTVVMDVPPQDIITKDNVSVKVNAVVYFRVVDPSKAVVEVED
                     YLYATSQLSQTTLRSILGQAELDELLSSREKINEELQSILDTHTDPWGIKVSQVEIKH
                     VDLPQEMQRAMAKQAEAERERRAKVINAEGEFQAATKLAQAAEIMNKEPITIQLRFLQ
                     TLREVAAENNSTTIFPVPIDLFKPFVKILENKAEQK"
BASE COUNT         1549 a         1150 c         1138 g         1657 t
ORIGIN      
        1 cgttgacgtt ttgcctgcca agaaagggga tgatattttc tatcaatccc agcctgtgga
       61 taaaaaagcc agtggttttg ccgccagctt gtcttttcgt gattccgatg gagacggctt
      121 tttaatgcct ggggaagaag ccgctttaca aattgattta aaacctctgt acgccgaaaa
      181 gcgcgacagc gtacggctcc aactcctatg ttctgcagaa agcagagaag tgagttacga
      241 tcacgaatta attttcaaag attgccaatc taatcaatgg aatcaaatga ttgttcccat
      301 aaaagcttta ccaaaattac aaaatcgcag tattgtgttc tggggtatca tcgaaaccaa
      361 atatggtttt caaatattac cagccagctt gatcatcaat ggtaaaaatc cggagaaaaa
      421 tatataagtt agttgaagga acttttttat attagagcga gtgaaattta acctataatg
      481 aaaactaatt gtcaaattta ctgcttatta tcattgttaa ctggattaaa tttggaaatc
      541 tcacggtcta attaaatgct atgaaaacta aaaataaagg aatgaatatg aaagcgaacg
      601 cattaactca aattatttta atgacggccg ccctcattat ctccagttgt tatactcagt
      661 tttccgcacc gatcgtccgc caagaaacct atcaacaatc agttaccgat acaacttacc
      721 aggaacaaga aacttatgag aattacacat ttgactcttc ttatttaaat gattatcgtc
      781 ttggttaccg tgatggttat tgggactcaa tgaatagtca actctggtta ggtttcaata
      841 tttactcgcc attttatggt ggctatcgct atcattcacc gtatctcgga tattatgatc
      901 cgtggtttta cgatccgttt tatgactggg gcttttatgg ttacttttcc ttctacggcg
      961 gttattatcc ctattatgga tattaccctc attacggcta ctatggctat tactatggcg
     1021 gataccccta tcataggcat cattggcatg gatatcggga tggaacctac tacaacgaag
     1081 gacgtgatga gttaaaagct gattatgtcc cgaagtcaac tcccagtggc agtcgtggtt
     1141 cttcaaatga tgctggttct caaagtggtg catcttacct gcctacttcc accagtgcca
     1201 taacgaccac gtccgtatca ggcgaaaaaa ctacggtgac taccaccaat tccggctcga
     1261 ccaaagctcg ttattcgccc aagacgacag gagcgggaaa tagnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnntcatcgt ccagcagctc gtcttcatcc agtagttcat
     1441 cttcatccgg cagtcgttct tcttccagct ctggtagtag ccgttcatct tcgcctaaag
     1501 cttcggggga ttcatcgaaa aacaatcgca aataggagtt aatatgtcca aattctgccg
     1561 gtattttttt attttggcga tgttggtgtt ttttttacca atgtcaattt ccgctcagca
     1621 tttcgatcaa gtaatgagta gcgaagccct tcgaccattc tggagtattt atggtatagg
     1681 agcttcatcg ttcaatttat tgaataattc tcacttggaa acggatattg gttcgactca
     1741 gtccaaccct gcttttctga caaatgttaa acggcctaaa ggcagttttt ccggaatcag
     1801 tattactcct aaaaatttga ctactcttgc caatgtgggg aaagactatt cggcttccac
     1861 cactaaatat aatgtttcct acataggatt tgcttatcct tttccggttt atcaaggtag
     1921 ttttgtaatg gcggcttcct attcaccggc ggcttattat tattcttcct catataccaa
     1981 aggaactacc ggcgcgactt attacgatgg ggacggaaat gctcacatca ttgatactga
     2041 ccttgaatat gatattaatg agtccgggac tttaaatgta ttccggtttg ctggtgccac
     2101 tgaatttatg aaaaatttta accttggttt gtcatttaat ttctacaacg gccagcgcca
     2161 atatgagtca actgaaatag gtgttgacgt caatgacgaa ttagatttta attctattat
     2221 ttaccaagaa aatatcaaac caagctattc tggattcaat atggatttcg gaatatgcta
     2281 tcaatcggaa aattataaaa tcggcttacg cctttcgact cctctgaaac ttaaagtgca
     2341 tgaggtgagc gaactgacgg agacttattc ttatgatact gtggattcta tctttccaaa
     2401 ttcttacgat ttcaaataca gaacgcgtta tcctcttgag atcgctccca attttgccat
     2461 aaaggttggt aaaatttcgc tgggtatgga tttcattttc cataactggc aaaagatcga
     2521 agtggattta ttggacgaaa tgagggaggt caaccgcgat ctctactgga atctgcgcaa
     2581 tacgaccgat atcggtgcgg ttctgaccat tccattcggc aattttgttt cgacgcgcct
     2641 agcttaccgc ttagtgccat cgccttatgc ggaatatgag cctgatgagg aatattacca
     2701 cgttatgggg gcgggtatcg aaacaattct caaagagtct atcatcatcg gctgtagttt
     2761 tcagcatgga ttcggcaatc tgtctaattt ctatcgctat tttgaaacga cgacaaccca
     2821 agcctacacc gaaaatcgct gctcaatctc gctggctgtg ctattctaaa ttaatttttc
     2881 tcacgatagt atcggctaaa aacactgaaa aagattaaaa gccctttgag attgatatta
     2941 cggtgagagc gatttcatct ttaatatttt acctccttct attgatgcaa ttttccatcg
     3001 cgggcgaaat cccacatatc tatcatattc caccaaaaac tttgattcaa aataatccgg
     3061 cagaaattac ctgtgtgatt acgccgggcg acagtcaatt aaaatgggtt aaaattttcc
     3121 tgcgcgatga ttcttctgag ttatataaag aatttaatat gcaatatcgg gatggcatct
     3181 ggtttctgcc tctaatcccg gaaatggtca gtggtaatta tttagattac tttatcatcg
     3241 cggagtttga agattttact gcgctggctt tccctcccga acgaccggct gaaaagccca
     3301 tcagggtcca aatagtcagc actaagaaaa tttaacctca ccgactttta aaaaaagatt
     3361 tgagattcac gcctatacgg attatattca atataattat gaggagtcgc ttttatttac
     3421 cgaaaatttt cttgatcttg ctggttggca gtattttgct gaccgcgact cctaaagaaa
     3481 tttgggtgct ggaagttgac ggagttatca atccggtttc agcttcctat atcaacgaca
     3541 atttgcgcaa agctcaaccc gatcaggtcg agtgtctgat aattcagatg gacactcccg
     3601 gcgggctaat gacttctatg cgggatataa tcaagaatat tcttaactca gaaattccag
     3661 ttgttgttta cgttagtccg cgcggggcgc agtgtgcttc cgcgggagta tttattgccg
     3721 tttcagccca ttttgtggcg atgtcgcccg gaacgaatat tggcgcggct catccggtca
     3781 atctgggtgg cggaggaatc ggcggccagc cagattctgc tagttcccag actatgattg
     3841 aaaaagccac taatgatgcg gtcgcttata tccgaagtct tgcccgcgaa cgtggtcgta
     3901 atgaagagtg gctggaaaaa gccgtgcgcg agagcgcttc cattaccgag accgaagccg
     3961 ttaaacttca ggtcgtggat gttgtagtag atgacttaga tgaactatta gaaattctcg
     4021 atgggaaaac tttcaaactt ccttccggtg aaaaaaccat ccacacccaa gatgcgacta
     4081 tccgattccg tccggttggc atccaccatc ggattctgga tgtaatcagt gatcctactg
     4141 tggcttatat tctactaatg ctgggctttt acggaattta ttttgaattg tctaatcctg
     4201 gtgcaatttt tcctggagtg ctgggtgcca tttttcttat tttagcattt ttcgctttcc
     4261 aggttctacc gatcaactat gctggtctgg cgttgattat tctcggtatt atactcttta
     4321 tacttgaagt aaaaataatt agctacggat tgttaaccat tggagggatt gttgcaatga
     4381 cactcggttc attaatgttg atcgatgtaa atcaagctcc tgaaatttta gaagccgtct
     4441 cgttaaaagt gatactgccg attgtaattt ttacggccgc ttttgttatt atatctctta
     4501 cgctagcact caaggctcat aaagccaaac caactaccgg aattgaagga atgattggcg
     4561 aaaccggcca gacaattacc gacgtagata aaaccggcat ggcaatcgtt catggcgaat
     4621 actggaaggt cagttccgat acacccattc ccaaaggaac ggaaattgtt gtgcttgatg
     4681 ttgatagaat ggttttgaaa gtcaaaccca aataaataac aagtaacaag taaggagatt
     4741 aagatgagca ttccattaat aactattatt atcctgctga tttttctaat ttccagcgca
     4801 attcgggttc tgcgtgaata cgaacgtggt gtcatattcc gcctaggccg cttgattgcc
     4861 gcaaagggac caggactctt tttccttatc ccggtagttg atcggatggt caaggtaagt
     4921 ctgcgaacag tggttatgga cgtaccaccg caggatatta tcaccaaaga caatgtatca
     4981 gtcaaagtca atgcggtggt ctatttccgg gttgtcgatc cctccaaagc cgtcgtcgaa
     5041 gttgaggatt atttatatgc cacttctcaa ttatctcaga caacgcttcg tagtatttta
     5101 ggtcaggctg aactcgatga actgctttcc agtcgtgaaa aaataaacga ggaattgcag
     5161 tcaattttag atactcatac cgatccctgg ggcatcaagg tttcacaagt cgaaatcaaa
     5221 cacgttgacc taccccaaga aatgcaaaga gcaatggcta agcaggctga agctgagcgg
     5281 gaacgccgcg ccaaagtcat caatgccgaa ggcgaatttc aggctgccac caaacttgcc
     5341 caagccgcag aaattatgaa caaagagcct attacaatcc aattacgctt tctgcaaact
     5401 ttacgcgaag ttgccgctga aaataattca acgacgattt tccccgtacc aattgacttg
     5461 ttcaagccat ttgtaaagat actggaaaat aaggcggagc agaaataagc ccggcggtaa
     5521 ttttttaagt agtccgttaa tttttactgg ataaattggt gactgaattg gttaagtgct
     5581 gataagtgct acta
//
LOCUS       DAOOMU010000111         5444 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_18030_length_5444_cov_16.802932, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000111 DAOOMU010000000
VERSION     DAOOMU010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18030_length_5444_cov_16.802932"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="PLI55_09005"
     CDS             complement(<1..872)
                     /locus_tag="PLI55_09005"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPB01007.1"
                     /translation="MKLKAQLVEYFKSNQADVRSQIIALVTEMVRQKTINVVTEKLPE
                     FPFLKMRGEEYRIAEIVQRELDKAGIPYEIFARAEGRPNVISRLGQNKSGQRLLMPAH
                     MDVVPAGEGWLTDPFEVVEKEGKLYGRGTLDNKGPLASILIAAQILKKLGIDQQLTGE
                     LMVAALSDEEAEDPDGINYGIGYLVEENLINPTMAIVPDIGGNMLEIDVAEKGRTVIK
                     ITALGKQAHGSTPEKGINAIFMMAKLVDELERLKFKYEVHPLLGNPTLNLGEIHGGVA
                     SNIVPGSCYIYLDIR"
     gene            complement(988..1887)
                     /locus_tag="PLI55_09010"
     CDS             complement(988..1887)
                     /locus_tag="PLI55_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPB01008.1"
                     /translation="MSNAYLGELAALLVAICWSFSSLSFEAAGKRIGSLQVNFIRLVL
                     AFIFLTLFNGIFRHQFWPRSFSAHAWFWLSLSGLVGFVFGDYFLFNAYVIVGARVSML
                     VMSLVPLITAVIGWLFLGEVMNLREILAMLVTICGVAIVIVGRNTENGKLKFSQPATG
                     ILYALGGAIGQAVGLVLSKYGMSDYNAFGATQIRVITGMIGFAVIFLILKRFPLLAKV
                     VHNRDAMKYTTIGAFIGPFLGVSLSLYAVQRTITGIASTIMAIVPVLIIPLEVLIKKE
                     KITFREVAGAIIAVGGVALYFIE"
     gene            complement(1964..4246)
                     /locus_tag="PLI55_09015"
     CDS             complement(1964..4246)
                     /locus_tag="PLI55_09015"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPB01009.1"
                     /translation="MNLNYLFLIAPIGSIIALVTAWIFYKLMLKNDPGNDQMQKIALA
                     VREGAFAYLKQQYKVVALFFLIIFVLFLIMSFGLNVQNKWVPFSFLTGGFFSALAGYF
                     GMSTATLASNRTAQGARKSLNAGLQIAFRSGAVMGLVVVGLGLLDISMWFAILYWLVP
                     SLGFDQMSLAAITTTMLTFGMGASLQALFARVGGGIFTKAADVGADLVGKVEAGIPED
                     DPRNPATIADNVGDNVGDVAGMGADLYESYCGSILATAALGVAAFQGQPHQKILAALL
                     APMIVAGVGNIFSVIGAFAVRTEEKADQRTLLKALSRGVNLSVVLIAIFTAILMYFLK
                     LPMGIVASVITGLLVGWTIGKITEYYTSQSYKPTRWIAEQSKTGPATVIIAGLSTGML
                     STGLPVLTAGIGIILSYAFAGGFGADPNHINLGLYGISFAAVGMLSTLGITLATDAYG
                     PIADNAGGNAEMSGLPKEVRQRTDALDSLGNTTAATGKGFAIGSAALTAMALIAAYIE
                     EIKAQFSHIINNPAILQNLLSEVNIQFDPGASLKTLQELVANNLHLPQWIEYYNVTLM
                     NPRVLVGMFLGSMVTFVFAALAMKAVGRAAGNMVEEVRRQFREKPGIMEGKDEPDYAS
                     CVAISTKGAQREMILPSLLVITVPVLTGLILGVAGVLGVLAGGLTTGFVLAVMLNNSG
                     GAWDNAKKYIEEGNLGGKGSDAHKAAVVGDTVGDPCKDTAGPCLNIVIKLMSTVSIVT
                     VGLAVKFGPVIGTWLGIGPR"
     gene            4516..>5444
                     /locus_tag="PLI55_09020"
     CDS             4516..>5444
                     /locus_tag="PLI55_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01010.1"
                     /translation="MKRFGLSYKLILASIVVLFMITLTWAEKPTLVIMDFEANGIPPE
                     EAKVFTEHLKVELFNSENYSIIEKSGINQKLQEEGFSGDKCATVDCLVQAGKFCGAEQ
                     AVGGSITKIGDTYSVMVRLVDVNHSQLLKTFAYDHKGDIISLLSDGTSKVAAKISEPK
                     KLNAEPVVAEKVGTVVEDTVIIAPARGKGGIVPALASCMIGPRVGLEMNEGNDTIYQS
                     EWIALGGSVVGSALTGYFAPLGSAISVGTRAYMAYDMGGKNNGLEGALASYCLGPRVG
                     NELHYRKIRTLEWLQLVPCVCIYPMIALPLEAY"
BASE COUNT         1611 a         1316 c         1114 g         1403 t
ORIGIN      
        1 cgtatatcga ggtaaatata gcaagaaccg ggcacaatat tagaagccac tccgccgtga
       61 atttcaccta aattgagagt tggattacca agtagcggat gaacttcata tttgaacttc
      121 aaacgttcaa gttcatcaac taatttcgcc atcataaaaa tagcattgat gcccttttcc
      181 ggcgtagagc cgtgcgcctg cttgccgagg gcggtaatct taatgaccgt acgacctttt
      241 tcggcgacat caatttccag catattgccg ccaatatccg gcacaatcgc catcgtcgga
      301 ttaatcaaat tctcttccac aagatagccg ataccataat tgatcccgtc tgggtcttct
      361 gcttcttcat cagaaagtgc cgcaaccatt aattcgccag ttaattgctg atcaattccg
      421 agttttttca aaatttgagc cgctatcagg attgatgcta gcggtccttt attgtcaaga
      481 gtcccgcggc catagagttt gccttctttc tcaacaactt caaatgggtc ggttagccaa
      541 ccttcccccg ctggaacgac gtccatgtga gccggcatca ggaggcgctg tccactttta
      601 ttctgaccta aacgcgatat aacattcgga cggccttccg ctcgagcaaa aatttcgtag
      661 ggaatcccag ctttatccaa ttcccgttga acgatttcag ctattcggta ctcttcacca
      721 cgcattttca ggaatggaaa ttcaggcaat ttttcggtta cgacattaat cgttttttgt
      781 cgtaccatct ctgtgaccag agcgatgatt tgggatcgaa cgtccgcttg attggatttg
      841 aaatattcga ctaattgcgc tttgagcttc ataaagcctc ctgataattt aaatgcgaca
      901 ttcttgtgag aaattagttg atttgcaaat acttatcaac aggtaaaaat ttaaaaatcc
      961 cgacatattc ttaaaacgac cataaattca ctcaatgaag tagagggcta ccccaccgac
     1021 ggcgataatc gctcctgcca cttctctaaa tgttattttt tctttcttta ttaaaacttc
     1081 taaaggaatt atcaaaaccg gcacaatagc cataatcgtg gaagcgattc cggttatagt
     1141 acgttgaacg gcatagagcg agagtgagac ccccagaaat ggtccgataa acgctccgat
     1201 agttgtgtac ttcatagcgt ctcgattatg aactactttg gccaaaagcg gaaagcgttt
     1261 caagattaaa aatattactg caaaaccgat cataccagta atgacccgga tctgagttgc
     1321 cccaaaggcg ttgtaatcac tcataccata tttgctaaga accaagccga cagcctgtcc
     1381 gattgcgcct cctaaagcgt atagaattcc ggtcgccggt tgcgaaaatt ttaatttgcc
     1441 gttttcagta ttccgcccga caattacaat tgccacaccg caaatcgtca cgagcattgc
     1501 cagtatttcc cttagattca tcacttctcc gagaaacaac cagccgatca cagccgttat
     1561 cagcggtact aatgacatta ccagcattga aacgcgtgct ccgacgatta cgtaagcatt
     1621 gaataaaaaa taatcgccaa atacgaagcc aaccaggccg gacaaagaaa gccagaacca
     1681 ggcatgagcg ctgaacgatc gcggccaaaa ctgatgccta aaaataccat tgaacagcgt
     1741 caggaaaata aatgccagta ctaagcgtat gaaattaacc tgtagggaac cgatccgctt
     1801 accagccgcc tcgaatgaaa gtgaagaaaa agaccagcat atagcaacca gcagagcggc
     1861 taattctccc aaataggcat tactcatcgc gttcggcatc aaatttaaaa ataaaaaaat
     1921 cggcgcaatc ttcggagact acgccgattt ttaaaatgtt caattatctg ggaccaattc
     1981 ccaaccaggt tccgataacc ggtccgaact tgactgctaa tccgacggtc acgatcgaaa
     2041 ctgttgacat caatttaatc acgatattga gacatggacc ggcagtatcc ttgcaaggat
     2101 cgccgaccgt atcgcccact actgcggctt tatgtgcatc cgaacctttg ccgcctaaat
     2161 taccttcctc gatatatttc ttggcgttat cccaggcacc gcccgaattg ttcagcataa
     2221 ccgccagcac aaaaccagtc gtcaaaccac cggctaatac acccaatacg ccggcaactc
     2281 ccaaaattag accggtcaaa accggtacag taatcactaa cagtgatggt aaaatcattt
     2341 cgcgctgagc gcctttggtg ctgattgcta cgcaactagc gtaatcgggt tcgtccttgc
     2401 cttccataat tccaggcttt tcacggaatt gacggcgcac ttcctcgacc atattaccag
     2461 cggcacgacc aactgctttc atcgctaaag ccgcaaaaac gaaagtcacc attgagccga
     2521 gaaacattcc taccagaacg cgcggattca ttaaagtaac gttataatat tcaatccatt
     2581 gcggtaggtg cagattgttc gccaccaact cctgcaaggt cttcagtgaa gcaccggggt
     2641 caaactgaat atttacttca ctcagcaagt tctgtaaaat cgccggatta ttgataatgt
     2701 gggaaaattg agctttaatc tcttcaatat aagcggcaat caaagccata gccgtcaaag
     2761 cggctgagcc aatggcaaag cccttgccgg tcgcggcagt agtattaccc agcgaatcca
     2821 gagcgtccgt acgctggcgc acttctttcg gcaatccgct catttcggca tttccaccgg
     2881 cattatcggc aattggtccg taagcgtcgg tcgccaaggt aattcccaat gtagagagca
     2941 tcccaactgc cgcaaagctg attccataaa gcccaagatt tatgtgattc ggatcagcac
     3001 caaatccacc cgcaaaagca taactcaaga taattccaat accggcggtc agaaccggta
     3061 atcccgtcga aagcatacca gtcgaaagcc cggcaataat gactgtcgcc ggaccggttt
     3121 tggactgttc agcgatccag cgcgtcggtt tataactttg tgaggtgtag tactcagtta
     3181 tttttccaat tgtccagccg accaataatc ccgttataac ggaagccacg atgcccatcg
     3241 gcaatttcag aaagtacatt aaaattgcag taaatatcgc aattaaaacg acacttagat
     3301 tgactccgcg ggaaagcgcc ttcaataatg ttcgttgatc ggctttttct tcggtgcgga
     3361 cggcaaaagc gccgatcaca gaaaaaatat taccaactcc agcgacgatc attggggcta
     3421 aaagtgccgc caaaattttt tgatgcggct gtccctgaaa agcggctacc ccaagcgccg
     3481 ctgtggctaa aatcgaaccg caataagatt cataaagatc ggcgcccatt ccggcgacat
     3541 caccgacgtt gtcgccgacg ttgtcggcaa tcgtggctgg attgcggggg tcatcttctg
     3601 gaattccggc ttcaacttta ccaaccaaat ctgctccgac atccgcggct ttagtaaaaa
     3661 taccaccgcc tactcgagca aacaacgcct gtaaagaagc gcccatccca aatgtcaaca
     3721 tcgtagtcgt aatcgccgcc agactcatct ggtcgaatcc cagacttggt accaaccagt
     3781 atagaatcgc aaaccacatc gaaatatcca acaatcccaa acccacaact actaagccca
     3841 ttacggcacc ggaacgaaag gcaatttgaa gtccggcgtt cagtgattta cgagcacctt
     3901 gggcagtgcg attggatgcc agtgttgccg tactcattcc gaaataacca gctaaagccg
     3961 aaaagaaacc acctgtcagg aatgaaaatg gcacccattt attctgaaca ttaagtccaa
     4021 atgacataat cagaaataac acaaaaataa tgagaaagaa taatgccacc actttatatt
     4081 gctgtttaag gtaggcaaaa gcgccctcgc ggactgctaa ggcaattttc tgcatttggt
     4141 cattacccgg atcgtttttc aacatcagtt tataaaaaat ccaggcggta accagggcaa
     4201 tgatcgaacc aattggtgca attaaaaata aatagtttag attcattctt aacaaacctc
     4261 ctttaagttg tgactaaaat cgtgcggacc caatccgacc ccaaattact tttactgaaa
     4321 attatcatta ttaaaataac tcggaaatat ataactattt cagctaattc tcaaaaaatt
     4381 atctgaaaaa taaaccataa aaccaactct cattggacta tatttccaaa ataatcagaa
     4441 tccaactcat ttttgattta aatctttttg tttaccagaa ttttcgctat tttatatcat
     4501 attaaggagt ttaatatgaa acgatttggt ctttcttaca agttaatctt agcctcgata
     4561 gttgtgctct ttatgataac tcttacttgg gcggaaaagc caactttagt tattatggat
     4621 ttcgaggcaa atggaattcc acccgaagag gcaaaggttt ttaccgaaca cctaaaagtc
     4681 gaattattta acagcgaaaa ctattctatc attgaaaagt ccgggattaa ccagaagctt
     4741 caggaagaag gattctccgg cgacaaatgt gctacagttg attgcctggt tcaggctggt
     4801 aaattctgcg gagcagaaca ggcagttggt ggctcaatta caaaaatcgg cgatacttat
     4861 tcggttatgg ttcgtttagt tgacgttaac catagtcaac tgctaaaaac atttgcttat
     4921 gatcataaag gcgatattat cagcctcctc tccgatggta catcaaaagt tgcggcgaaa
     4981 attagtgaac cgaagaagtt aaacgctgaa ccggtggttg ctgaaaaagt cggcacggta
     5041 gtcgaggata cggttattat tgctcccgca cgcggcaaag gtggaattgt tccggcgctg
     5101 gcaagttgta tgattggacc gcgggttgga ttagagatga acgaaggcaa tgatacaatt
     5161 tatcaaagtg aatggatcgc attaggtggt tccgttgtcg gcagtgcgtt gactggttat
     5221 ttcgcaccgt tgggaagtgc aatttcagtt ggaacacgag cctatatggc ttatgatatg
     5281 ggcggtaaaa acaacggtct cgaaggtgcc ctcgcgagtt attgcctcgg gcctcgagtc
     5341 ggtaatgaat tacactatcg taagattcgg acattggagt ggcttcaact ggttccctgt
     5401 gtctgcattt acccgatgat tgcccttcca ctggaagcct acaa
//
LOCUS       DAOOMU010000112         5211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_19098_length_5211_cov_7.179209, whole genome shotgun sequence.
ACCESSION   DAOOMU010000112 DAOOMU010000000
VERSION     DAOOMU010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19098_length_5211_cov_7.179209"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2246
                     /locus_tag="PLI55_09025"
     CDS             <1..2246
                     /locus_tag="PLI55_09025"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB01011.1"
                     /translation="LMKSADQYDLSVQYGERFINNILGVQMTGNMEKRIRSNERITLD
                     YNQNPTQSDAGYFIDDFILEFTDEIRKRDGFSLLLDINTPDEGSIRFNNVFGRTKRDY
                     FWYSRDYPSNGGGDQSGNPEYDYRDREQEIKTFSSSIHGDNKLFNFGINWGLSFSESE
                     SRHTFDYEAYFVEPNGMNPSPKVQSNPKQLIDYAVNDFSAANLYWVYYRPQHNYDKER
                     TLYLNIDRQYSFAQTFSGQFKFGGKYKIKDRSNVRSEYFTPYYLGKWQKYEKLPDGTI
                     REKDFSGTYFEEWYNKEVLFIPISEFYSSHESRNIYDSYALDPLIDRDRMRQWWYLNR
                     YGVDVTGNIDEVWTNPLIKYDDYDITERVSAGYLMNTLNIGQAVTFIAGVRVENEDND
                     YISCYMPNMVSGFPVPANSILDTTSSSSQTVWLPNFNLAISPFSFMKLRLAAYKALAR
                     PDFNMRLERYIAGRPAEVGTSLQVYVGNPNLKTAQAWNYEINTSFFGDAIGLISVSAY
                     YKEIEDMFHMLNNFNTTAVKDDQGVYQDTLMQRFGIKWSSQMGSAAYNLTLPYNSPKP
                     TKVWGFEFEHQINFTHLPGFLKYFVLSYNASVVRSEAYIWASKIDSVYYDPPGPAPAT
                     YKKFYVLTERKQKLEGMPEFFGNVALGYDIKRFSGRISVFHQGEYNISYSASGLSDRV
                     NNPFTRVDLTLKQGLNDNISVFLNVSNLTDIEEGSSIYNRVYDRKLFRNSERYGITAD
                     FGINIKL"
     gene            2355..>5211
                     /locus_tag="PLI55_09030"
     CDS             2355..>5211
                     /locus_tag="PLI55_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01012.1"
                     /translation="MLKEKIGAIVIITFLLFAGLYGQDVIVTLVPYDGSEATFLQNQI
                     KADTLASGGLLANHVYELKRDEYYFANATFTVPPGATLRLRAQEGTGKKPIVYLWETG
                     TGSSPTRPPGWFVTLNGGNIELKDICIAGYYEWEPERVSGVQGGLISTSAAGGSIVID
                     GAILSNTNGNHIRTNQNVFKVKVTNTIFANMGALTTSNLGAGKGLDLRDVRVDSLILV
                     NNTFVNYQDRAVRHYTTDPTKGPIMYGLIDHNTFINGMGYHGLLSLGNVGPEIIITNN
                     LFMDAFALGEDSTDATRAAEWANTGETYPNGNNKITWIFSTPNDTTQWTVSKNYWAIS
                     DSGWAFLNDFNFGPGSPLSDHIYGKLGSGASQAFTQTNIALGNTPRLMTNMMRWYESP
                     TGGNKTKNTPSDLFVVERDDYDRRLLEYYRDTLDASYPETHSAFVGADNNYPVGDLNW
                     FPELKAQWEQGVIIQPTEVVVILEPYDGESEATFLQNQIKADTLASGGLLANHVYELK
                     RDEYYFANATFTVPPGATLRLRAQEGTGKKPIVYLWETGTGSSPTRPPGWFVTLNGGN
                     IELKDICIAGYYEWEPERVSGVQGGLISTSAAGGSIVIDGAILSNTNGNHIRTNQNVF
                     KVKVTNTIFANMGALTTSNLGAGKGLDLRDVRVDSLILVNNTFVNYQDRAVRHYTTDP
                     TKGPIMYGLIDHNTFINGMGYHGLLSLGNVGPEIIITNNLFMDAFALGEDSTDATRAA
                     EWANTGETYPNGNNKITWIFSTPNDTTQWTVSKNYWAISDSGWAFLNDFNFGPGSPLS
                     DHIYGKLGSGASQAFTQTNIALGNTPRLMTNMMRWYESPTGGNKTKNTPSDLFVVERD
                     DYDRRLLEYYRDTLDATFSSSSPAYTGADKGYPVGDLNWYPVLKAKWLKGEDAAIAEY
                     TSTKASKFMLKQNYPNPFNPTTQIAFDLDKSGYTTLTI"
BASE COUNT         1531 a          875 c         1269 g         1536 t
ORIGIN      
        1 atttgatgaa gtcggctgat caatatgatt tatctgttca atatggtgaa aggtttatta
       61 acaatattct gggcgtgcag atgaccggta atatggaaaa gcgcatcagg agcaatgaga
      121 gaatcacctt agactacaat cagaatccga cacagtccga cgcaggttat tttattgatg
      181 attttatact tgagtttact gatgaaatta ggaaaaggga tggatttagc cttttgctcg
      241 atattaatac accggacgaa ggctcgatac gttttaataa tgtttttgga agaaccaaac
      301 gggattattt ctggtattca agggattatc cttccaatgg agggggcgat cagtctggta
      361 atccggaata tgattatcga gatcgcgaac aagaaataaa gacttttagc agttctattc
      421 acggtgataa taaactattc aactttggga taaattgggg cttatctttt agtgaatcgg
      481 aatcgcgtca taccttcgat tatgaagcat attttgtcga gcctaatgga atgaatcctt
      541 caccaaaagt tcaatctaat cctaagcaat taattgatta tgcggtcaac gatttttcgg
      601 cagctaattt atactgggtg tattatcgtc cgcaacataa ttatgataaa gaacgaacat
      661 tatatttgaa tattgatcgg cagtattctt ttgcacagac tttttcaggt caatttaaat
      721 tcggcggtaa gtataaaata aaggacagat caaatgtaag aagtgaatat tttaccccat
      781 attatcttgg taaatggcaa aagtatgaaa aactgcccga tggtacgatt agagaaaaag
      841 atttctccgg tacttatttt gaagaatggt acaataaaga ggttctcttt attcctattt
      901 cagaattcta tagtagccac gaatcaagaa atatttatga ttcttatgca ttagatccat
      961 tgattgatag agataggatg cggcagtggt ggtatctaaa ccgatatggg gttgatgtca
     1021 ctggcaatat agatgaagta tggactaatc cattgataaa atatgatgat tatgatatta
     1081 ccgaaagggt cagtgccggt tacctaatga atactttaaa tattggtcaa gcggtaactt
     1141 ttattgccgg tgttcgggta gaaaatgaag ataatgatta tatctcctgt tatatgccta
     1201 atatggtgtc gggttttcct gtgccagcca actcgattct cgataccact tccagtagtt
     1261 cccaaactgt ttggttgcca aattttaatc tggcaatttc gccattcagt tttatgaaat
     1321 tacggttagc cgcatataaa gctcttgctc ggcctgattt taatatgcgt ctggaaagat
     1381 atatagccgg tcgtccggct gaggttggga ctagcttaca agtttatgtt ggcaatccaa
     1441 atttgaagac cgcccaggca tggaattatg agattaacac atcatttttt ggcgatgcta
     1501 ttggattaat ttcggtttcg gcctattata aagaaatcga agatatgttt cacatgctga
     1561 ataatttcaa tacgacagcg gtgaaagatg atcaaggagt ctatcaagat acccttatgc
     1621 aacgctttgg gataaaatgg tcaagtcaaa tgggctcagc ggcttataat ttaacgcttc
     1681 cttataattc gcctaaaccg accaaagtat ggggatttga attcgagcat cagattaatt
     1741 ttactcatct acccggattt cttaaatatt ttgtattaag ctataatgct tctgtggtac
     1801 gttcagaggc ctatatttgg gcttcaaaaa ttgattcagt ctattatgat ccgccagggc
     1861 ctgcaccggc aacctataag aaattctatg tgcttacaga aagaaagcaa aaattagagg
     1921 ggatgccgga attctttggt aacgttgctt tgggttatga tataaagagg ttttcgggaa
     1981 gaatttcggt tttccatcag ggggaatata atatttccta ttccgcttct ggtttaagtg
     2041 atcgggtgaa taatccattt acgcgcgtag acctgaccct gaaacaggga ttaaatgata
     2101 atatttcggt attccttaat gtatccaatt taaccgatat tgaagaaggc agttcaatct
     2161 ataatagagt atatgatcgg aaattgttta gaaatagtga aaggtacgga ataactgccg
     2221 attttggtat taacataaaa ctttgatagc aatgaaaccg gaaaatattt ttattagaaa
     2281 ggaggttgta atcaaaagta aagagttaat tattgatcta ttaaagcgaa aaaattagca
     2341 tgaaaggaga aattatgtta aaggaaaaaa ttggggcaat tgttatcatt acttttctgt
     2401 tatttgcagg actgtatgga caggatgtca ttgttacgtt agtaccgtat gatggttcgg
     2461 aagccacatt tctgcaaaat cagataaagg ctgatacatt agcctcgggt ggattattgg
     2521 caaatcatgt atatgagctt aaaagagatg aatactattt tgccaacgct accttcacgg
     2581 tgccgcctgg tgcgacctta cgtttgagag cacaggaagg cacaggcaag aagccgattg
     2641 tatatttgtg ggagacaggc acgggttctt cgccgacgcg gccgccgggc tggtttgtga
     2701 cattgaatgg tggtaatatt gagttgaaag atatttgtat agctggttat tatgaatggg
     2761 agcctgaacg ggttagcgga gtacagggtg gtttgatcag cacctcagcc gcgggaggtt
     2821 cgatagtcat agatggagct atattgtcta ataccaatgg taatcatatt cgtactaatc
     2881 agaatgtatt taaggtaaag gtgacgaaca cgatatttgc gaatatggga gcattgacga
     2941 cctcgaattt aggtgctggt aagggcttgg atttgcgaga tgtacgagtt gattcgttga
     3001 tattggtcaa taatacattt gtgaattatc aggatcgtgc ggtacgacat tatacgacgg
     3061 atccgacgaa gggtccgata atgtatggct tgatagacca taataccttt atcaatggga
     3121 tgggctatca tggtttatta tcgcttggca atgtagggcc ggagataata atcacgaata
     3181 atttatttat ggatgccttt gcattaggtg aggactcgac ggatgcgaca cgagcggcgg
     3241 aatgggctaa cacaggagag acctatccga acggtaataa caagataacg tggatatttt
     3301 cgacgccgaa tgatacgacc cagtggacgg tatcgaagaa ttattgggcg ataagtgatt
     3361 ctggctgggc atttttgaat gattttaatt ttgggccggg ctcgccgtta tcggatcata
     3421 tatatggtaa gttaggttcg ggggcgtcgc aggcatttac gcagactaat atagcgttag
     3481 gcaacacgcc gcggttaatg accaacatga tgcgctggta cgagagcccg acaggaggca
     3541 ataagactaa gaatacgcct tcggacttat ttgtagtaga gcgtgatgat tatgatcgcc
     3601 ggttactgga atactaccgc gataccttgg atgcatccta tcctgagact cattctgcct
     3661 ttgtaggagc cgataataat tatcctgtgg gtgacctaaa ttggtttccc gaattaaaag
     3721 ctcaatggga acagggcgtt attatccagc ctacagaagt cgtagtaatc ttggagccgt
     3781 atgatggtga aagtgaagcc acatttctgc aaaatcagat aaaggctgat acattagcct
     3841 cgggtggatt attggcaaat catgtatatg agcttaaaag agatgaatac tattttgcca
     3901 acgctacctt cacggtgccg cctggtgcga ccttacgttt gagagcacag gaaggcacag
     3961 gcaagaagcc gattgtatat ttgtgggaga caggcacggg ttcttcgccg acgcggccgc
     4021 cgggctggtt tgtgacattg aatggtggta atattgagtt gaaagatatt tgtatagctg
     4081 gttattatga atgggagcct gaacgggtta gcggagtaca gggtggtttg atcagcacct
     4141 cagccgcggg aggttcgata gtcatagatg gagctatatt gtctaatacc aatggtaatc
     4201 atattcgtac taatcagaat gtatttaagg taaaggtgac gaacacgata tttgcgaata
     4261 tgggagcatt gacgacctcg aatttaggtg ctggtaaggg cttggatttg cgagatgtac
     4321 gagttgattc gttgatattg gtcaataata catttgtgaa ttatcaggat cgtgcggtac
     4381 gacattatac gacggatccg acgaagggtc cgataatgta tggcttgata gaccataata
     4441 cctttatcaa tgggatgggc tatcatggtt tattatcgct tggcaatgta gggccggaga
     4501 taataatcac gaataattta tttatggatg cctttgcatt aggtgaggac tcgacggatg
     4561 cgacacgagc ggcggaatgg gctaacacag gagagaccta tccgaacggt aataacaaga
     4621 taacgtggat attttcgacg ccgaatgata cgacccagtg gacggtatcg aagaattatt
     4681 gggcgataag tgattctggc tgggcatttt tgaatgattt taattttggg ccgggctcgc
     4741 cgttatcgga tcatatatat ggtaagttag gttcgggggc gtcgcaggca tttacgcaga
     4801 ctaatatagc gttaggcaac acgccgcggt taatgaccaa catgatgcgc tggtacgaga
     4861 gcccgacagg aggcaataag actaagaata cgccttcgga cttatttgta gtagagcgtg
     4921 atgattatga tcgccggtta ctggaatact accgcgatac cttggatgcg actttttcga
     4981 gttcgtcacc ggcttatact ggtgccgata agggatatcc ggttggtgac cttaactggt
     5041 atcctgtttt gaaagctaaa tggttgaaag gtgaagatgc ggctattgct gaatatacct
     5101 ctacaaaagc gtcaaaattt atgctcaagc agaactatcc gaatccgttc aacccgacta
     5161 ctcagattgc atttgatctg gataaatccg gttatacgac tctaacgatc t
//
LOCUS       DAOOMU010000113         5177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_19260_length_5177_cov_21.397111, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000113 DAOOMU010000000
VERSION     DAOOMU010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19260_length_5177_cov_21.397111"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PLI55_09035"
     CDS             <1..957
                     /locus_tag="PLI55_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB01013.1"
                     /translation="LGLFFDFRNNVVYNWAGSSPGYNADKEPISRYNFIGNVYILGPE
                     SSTSSKLFKEDAINAYAYFEDNMFNGVVPEDQWSLVKFNGFTTDQIKAYKQRSSLIPM
                     EPVVTTSPEQAYLDVLASAGCSFPKRDTIDCRIIQDVINTTGHSIDSTSAQPEGGWPL
                     LSSLPAPTDTDHDGMPDDWEIAHSLNPADAADRNLLDENGYTRLENYLNGLVEQNPNR
                     VVSKPNVVADFRLAQNYPNPFNATTTIQFSLFKTGLAEIRIYDLAGQVVKTYPRQSLM
                     PGEYRLVWQGDNDAGKPVASGIYFCVMQFASNRQILKMQIIR"
     gene            1000..4305
                     /locus_tag="PLI55_09040"
     CDS             1000..4305
                     /locus_tag="PLI55_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB01014.1"
                     /translation="MLNNKPIIFFFCTFLVLAVGFTSNLQGQNRSRISGSVRDAETGE
                     PLIGVNIIIEETDLGAATDTDGNYIIINVPVGTHRVRASMIGYETKIFTNVMVSADRI
                     TRLDFELHRTVIQGQEVIVTAKRDELHKEVSTTQMVVDAMQISDATGIREINAFLAKL
                     PGIGEQNGYLTIRGGSADQTGMMVDGLSYVNAATGNAESSIPLSAIEQVSLLSGGYNA
                     EYGNFRSGLINITTKSGSSKDYHGTFSYSRDNAHIRRFGDSFYDAHNAALRSYLDPEV
                     AFIGTNTAWKDDAYLRDQYPSFTGWITQAKNYNIGKTEDQWATPFDYYLLACWMFMTI
                     PDYKGLANLSDSMKQVIGYQEVSKEQRKLFTQHHNREEYPDWNFDGGFGGPVPLIGKA
                     LGNATFYLSFNAKERHFVMPVSRNSDETYTTLLTMKSQPTKKTTLTYNGLYKYEDGVS
                     PIRPASGDYPDASRQGGFMPVNNIKYIHKNPEYWYDPPFFPIMSQRTAVNGVTLNHII
                     NKTTFWELKLSALQIRDNSPTGNTRDMRAITHFGPFVVNEMPYGKLLDGTNRVTGIFN
                     GDTLSYKFPNYDALPGVAYRFRRKEGDLYNNVRVGQYGAKFDLSSQIGRHNFVKTGFE
                     YNYIDLDHNLWMMWNENAYNVYEFNYHRTPSQTGIYVQDQLNFESMIANIGLRFDYYY
                     GGGGKWPTGDPFATEAFTPQSYASDSVLFDYLKRGKSYIWDTWVRYDSLHPGFLQPIK
                     NHYALSPRIGISFPVTEQSKFYFNYGHFRSNPPYYSMYLYRYRYTKNGLYDMSNPNLE
                     PPRTISYELGIAMTLFQTSVLKMSWYSKDVTGQHGEVTYQNASGTINYNAWANNEYQD
                     IQGIEINFSKSDASWLTGWINFNYMLKKTGLTGRSLITDVTINNDQEGLYAGQETRSL
                     PQPKINANLTVRSFDKWGPKISGIYPLADWTITLFAEWQAGEYFTWNPLNELHVNNNM
                     RWPDYYMVDLKVDKKFNLFGLTASFYVDISNLFNFKTTLMDDGYCFVREDGDLEEWSD
                     FVKYFGSLRLPMYESAKYDALRQQYPGMYIAGNDRIGELRSKEKPYINDPNYSFWLYE
                     QPRDIWFGLRFSF"
     gene            4333..>5177
                     /locus_tag="PLI55_09045"
     CDS             4333..>5177
                     /locus_tag="PLI55_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01015.1"
                     /translation="MKNYRENNLRINFSLILLAILCAGQFGFSQIQPELATALIKMGH
                     VWCGVTANGDKGSFEYRAGFFPNDYDIIAIRGQELDAWAGAGFRLAATNWQDPVYPDS
                     IHKVAIYGPTNDYMRTGKVIVPLTNYVRYTYPEQIINFNPINLEMFGTYDPSKFGEET
                     YDQIVEVTTQNILGVNIHRKILAWTQNYNDDYIIVDVTFTNVSEDTLHDFFINMESNG
                     VNAFRSNGSNPSPPSNERFNPTIVWQHYYGGRVGDSLRVFYEYSADDPTIAGDNMGAP
                     VTAQGG"
BASE COUNT         1413 a         1183 c         1160 g         1421 t
ORIGIN      
        1 ctcggtttat ttttcgattt ccgcaataat gtggtttaca attgggctgg ttcatccccg
       61 ggatataatg ctgacaagga accaattagc cgttacaatt tcatcggcaa tgtttatatc
      121 ttagggccag aatccagcac cagtagtaaa ctatttaaag aagatgctat taacgcttat
      181 gcttattttg aagataatat gtttaacggc gtcgtcccgg aagaccagtg gagtttggtc
      241 aaattcaacg gttttactac cgaccagatc aaggcttaca aacagcgctc ttctctaata
      301 ccgatggagc cagtcgtgac gacctcgccg gagcaagctt atcttgacgt cctggcttcc
      361 gccggttgca gttttccaaa acgcgacacg attgactgtc ggatcataca ggatgtgatt
      421 aacactaccg ggcattcgat tgatagtacc agcgcccaac ccgaaggcgg ttggcctctt
      481 ctaagcagtt tgccggcgcc aaccgacact gatcatgacg gtatgcccga cgactgggaa
      541 attgctcata gtttgaatcc cgccgatgcc gctgaccgca atttgctaga tgagaatggc
      601 tatacccgtt tggaaaatta tttgaacggt ctggtcgagc agaatcctaa tcgggttgtt
      661 tccaaaccga atgtagtcgc tgattttcgg cttgcccaaa attatcccaa tcccttcaat
      721 gcgacgacta ccatacagtt ttcattattc aaaaccgggc tggctgaaat caggatttat
      781 gaccttgccg gtcaggttgt gaaaacttat ccgcggcaaa gtctgatgcc gggtgaatac
      841 cggctagtat ggcaaggcga caatgatgct ggtaaaccag tggcttccgg aatttatttc
      901 tgcgtaatgc aatttgctag taatcgccaa attttgaaaa tgcagatcat tcgttaaaaa
      961 aataatgttg gaaaataaca cacggcttta ggagtagtta tgttaaacaa caagccgata
     1021 atttttttct tctgcacctt tttagtcctt gccgtaggtt ttacgagtaa tttacagggg
     1081 cagaaccgca gccgaatcag cggcagtgta cgcgacgccg agaccggtga accgctgatc
     1141 ggagttaata ttatcattga agaaactgat ttgggcgccg ccacagatac cgacggaaat
     1201 tatatcatca ttaacgtacc ggtcggcact catcgcgtgc gggcttcaat gataggttac
     1261 gagaccaaaa tatttacgaa tgttatggtt tctgccgatc ggattactcg cctcgatttc
     1321 gaactgcatc gcaccgtcat ccaggggcag gaggttattg ttactgctaa gcgtgatgaa
     1381 ctgcacaaag aggtttccac cacccagatg gttgttgatg cgatgcagat cagcgatgct
     1441 accggtattc gggaaatcaa cgccttcctg gctaaactac ccggaattgg tgagcaaaac
     1501 ggctatctga ctattcgggg cggttcggcg gatcaaaccg ggatgatggt tgacggatta
     1561 tcgtatgtca atgccgccac cggcaatgcc gaaagcagta tcccgttgag cgctatcgaa
     1621 caagtctctt tgctttcggg tggttataat gccgaatacg gcaatttccg ttcgggattg
     1681 atcaacatca cgaccaagtc gggaagctcc aaagactatc acggaacttt cagttactct
     1741 cgtgataatg cccatatccg ccggttcggc gattcatttt acgatgctca taatgcggct
     1801 ttgcgctctt accttgatcc tgaggtggct tttattggga caaacaccgc ttggaaagac
     1861 gacgcttact tacgtgatca gtatcccagt tttacgggct ggatcacgca agccaagaac
     1921 tataatatcg gtaaaaccga agaccagtgg gcgactccgt tcgactatta cctgctggct
     1981 tgctggatgt ttatgaccat accggattat aaaggattgg ctaatctttc cgattcaatg
     2041 aaacaggtta tcggttacca ggaagtatcc aaagaacaaa gaaaactctt cacccagcat
     2101 cacaaccgag aagaatatcc cgattggaat ttcgacggcg gttttggtgg tccggtgcct
     2161 ctgatcggca aggcattagg taacgctact ttttatctat catttaatgc caaggagcgc
     2221 cactttgtta tgccggtttc gcgcaacagt gacgagacct atacgacttt actaactatg
     2281 aaaagccaac cgactaagaa gaccactcta acctataatg gcctttataa atacgaagac
     2341 ggggttagcc cgattcggcc ggcttccggt gattatcccg atgccagtcg ccagggtgga
     2401 tttatgccgg tcaataacat caagtatatt cataaaaatc ccgaatattg gtatgaccca
     2461 ccttttttcc ctattatgag tcaaagaact gcagtgaacg gcgtaacctt gaaccacatt
     2521 atcaataaaa cgactttctg ggaactcaaa ctcagcgctt tacagattcg ggacaattct
     2581 ccgactggca acacgcgcga tatgagagcc attacccact tcggaccctt tgtagtcaat
     2641 gaaatgccgt acgggaaatt actcgacggg acgaatcgcg tcaccggaat attcaatggc
     2701 gatactctta gctacaaatt ccctaattat gacgctctgc caggcgtcgc ttatcgcttc
     2761 cgccgcaaag agggcgattt gtataataat gtgcgagtcg gccagtatgg agcgaagttc
     2821 gatctatctt ctcagatcgg acgccacaat tttgtcaaga cgggctttga gtataactat
     2881 atagatttag atcataatct atggatgatg tggaacgaaa acgcttacaa tgtttacgaa
     2941 ttcaattacc atcgcactcc atcccagacc ggcatttacg ttcaagacca attgaatttt
     3001 gaatctatga tcgccaatat cggtttgcgc tttgactatt attacggcgg cggcggtaaa
     3061 tggcctactg gcgatccatt tgctaccgaa gcttttaccc cccagagtta tgcgtccgat
     3121 tcggttcttt ttgattattt gaaaagagga aagtcttaca tctgggatac ttgggttaga
     3181 tatgattcac tccatcccgg cttcctgcaa ccgattaaaa atcattatgc tttgagtcca
     3241 cgtattggga tttccttccc agtcacggaa caatcgaaat tttatttcaa ctatggacat
     3301 ttccgctcca atccacctta ctattcgatg tacctgtatc gttatcgtta taccaaaaac
     3361 gggctatatg atatgtcgaa tcccaatctg gaaccgcccc gtacgatttc ctacgaattg
     3421 ggaattgcga tgacactttt tcaaacgagt gttttgaaaa tgtcctggta ttccaaagat
     3481 gtcaccggac aacatggtga agtaacctac cagaacgcca gcggcacgat caattacaat
     3541 gcttgggcta ataacgaata tcaagatatt cagggaattg aaattaattt ttccaaaagt
     3601 gatgccagct ggctgaccgg ctggatcaat tttaattata tgctaaaaaa gacaggcttg
     3661 accggacgaa gcctgattac cgacgttacc atcaataacg atcaggaagg cttatatgcc
     3721 ggtcaggaaa cgcgcagttt gccacaacct aaaattaatg ccaatctaac cgtgcgatca
     3781 ttcgacaaat ggggcccaaa aatttcaggt atttacccgc tggcggattg gacaattact
     3841 ctcttcgcag aatggcaagc cggtgaatat tttacctgga atccgctgaa cgaacttcat
     3901 gtcaacaaca atatgcgctg gcccgattat tatatggtag atttaaaagt tgataagaag
     3961 tttaacttgt tcggattaac agcctctttc tacgttgata tcagtaattt attcaatttt
     4021 aagaccacct tgatggatga tggatattgt tttgtcagag aagatggtga tctggaagaa
     4081 tggagtgact tcgtcaaata ttttggctcg cttcgtctcc cgatgtacga gtctgccaaa
     4141 tatgacgctc ttcgtcagca atatcccgga atgtacattg ccggcaatga tcggatcggc
     4201 gaattgcgct caaaagaaaa accatatatc aacgatccca actattcatt ctggctctac
     4261 gaacaaccgc gcgatatttg gtttggtctg aggtttagtt tttaaggatt gatttttgaa
     4321 ggagtatttg acttgaaaaa ttacagagag aacaacctga ggattaattt ttccttgatc
     4381 ttgctggcaa tattatgcgc gggtcaattc ggcttttccc agattcagcc ggaactcgcc
     4441 acggctttaa tcaaaatggg tcacgtttgg tgcggggtga ccgccaatgg tgacaagggt
     4501 agtttcgaat atcgggccgg ctttttccct aatgattacg atattatcgc tattcgggga
     4561 caggaactcg atgcttgggc gggtgccggt tttcggctgg cggctaccaa ttggcaggac
     4621 ccggtttatc ccgactccat acataaagta gctatttatg gacccacaaa cgattatatg
     4681 cgcaccggca aagtaattgt cccattgacc aattatgtcc gttatactta tcccgaacaa
     4741 ataattaatt ttaatcccat taatttagaa atgtttggga cctacgaccc aagtaaattc
     4801 ggcgaagaga cttacgacca aatcgtcgag gtaaccactc agaacattct gggagtcaat
     4861 attcatcgca agattttagc ctggactcaa aattataatg acgactatat catcgtagat
     4921 gtcactttca ccaatgtcag cgaggatacg ctccacgact tttttatcaa tatggaatcg
     4981 aacggcgtca atgcttttcg ttccaatggt tcgaatcctt cgccgccctc taatgaacgc
     5041 tttaacccta caatcgtctg gcaacattat tacggcggtc gggtcgggga ttcgttgcgg
     5101 gtattttacg aatacagcgc cgatgatccg acaattgccg gcgacaatat gggcgcacca
     5161 gttaccgcgc agggcgg
//
LOCUS       DAOOMU010000114         5176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_19268_length_5176_cov_5.792033, whole genome shotgun sequence.
ACCESSION   DAOOMU010000114 DAOOMU010000000
VERSION     DAOOMU010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19268_length_5176_cov_5.792033"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..1677)
                     /locus_tag="PLI55_09050"
     CDS             complement(328..1677)
                     /locus_tag="PLI55_09050"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPB01016.1"
                     /translation="MKRNTKITMACNNLLGLVLMILILGQFTSILIASDTSQAAYYPD
                     PTQYPDHIILSWSDDPISTQSVTWRTDTSISTAYAEIARADASPNFVYQAQRIPAKTE
                     LLHFDGKSANYHSVTFTDLQSNTLYLYRVGLERRWSEWFQFRTADKQPMPFKFIYLGD
                     AQKNILFLWSRSIRTAYKTSPDARFIIHCGDLVQSAYDYQGWNEWFTSGSWINATIPI
                     MPVIGNHEYSGKSDEEDLFTPYWRTQFTLPLNGITELPETNYYFDFQGLRMIVLNSTR
                     YKLEQAQWLEEILKNNPNRWTIIAFHHPIYCSKEGRGILEIRDLWNPIFKKYKVDLIL
                     NGHDHTYARGNNPERLPIRPNDPTGPVYVVSVSGPKLYNITKNRWMDRAAENTQLYQI
                     ISINNNILDYKAYTVTEELYDAFQIIEKSKGYKVFIDLQPKNYPERMFINTLIAPND"
     gene            complement(2050..3237)
                     /locus_tag="PLI55_09055"
     CDS             complement(2050..3237)
                     /locus_tag="PLI55_09055"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HPB01017.1"
                     /translation="MLIALIASVQCEELQLNFFRRPELEQLKNALVEPEGELLSYKKT
                     LMCSANLLLKEKSVSIVDFPCPNSNIDRHDYYSESTYWWPNPKDLDGPYIRKDGVRNP
                     NRFLKHKEALNHLCQSTLTLSASAYLFDNIQYAEKAKELIYVWFVDSATRMTPHAKYA
                     QVIPNSDKQRGVGILDTRRLIFVVEAIQILKLAGYWDVDLEIELTKWFSEYLDWLLYS
                     EYGKDERQRGNNHSTWYAVQVITYANYLGNKIVANETWDYVCNNLLARQIEADGKMPL
                     EMDRTQSLGYFIFNLEAWSYLSRLAENNHINLWKFQNAKGGSLAKSVQYILPYLERPD
                     TWPLEQINSIRRHEESFLLFGGKALENSKMVEIYCSVMSKEIIKEEVFLFILNMMVLS
                     GIL"
     gene            complement(3285..>5176)
                     /locus_tag="PLI55_09060"
     CDS             complement(3285..>5176)
                     /locus_tag="PLI55_09060"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPB01018.1"
                     /translation="PAEYIEKILITIPQVSKKSAETSLNVLLNNSRDRAVEGELLAHL
                     IDVNGKVVATVKQNLKLVTGNQEATIIFPKISNPSLWSPVSPNLYTIEVSLNIDGKIV
                     DEMTEKFGYRWFEFKEHGPFYLNGEKFLLRGTHRHEDWAGFGNAIPNELHRKDMQMIK
                     EIGANFVRLAHYPQDPEIYRACDELGLLVWDEVPWCRGGMGGEKWKQNTKNMLKDMII
                     QNYNHPSIIIWSLGNELDWLPDFENGGNMDSLIIFFSEMNDLVHKLDPYRLTATRKFA
                     EGSNIVDVYSPSIWAGWYSGVYKSYEKILNQSRYEFKHFFHAEYGGSSHLGRHVENPI
                     TGEGFVDLEDWEEEVNQVKVQNISQYGDWSENYIVDLFDWYLHISENLDWFAGNAQWA
                     FKDFATPLRMENPIPYMNQKGLVDRAGNPKDAYYVFKSYWTKTPEFCYIESHTWTERN
                     GPKNVPREVCVYSNASQVELIVNSTSLGTRERNIHDFPACGLYWDVIFKNGQNKLVAV
                     GYNSQGISCRDSIIINYSTKKWGKPDRITLSSYQLSNGRLQIEALVVDEDSHQCLDYN
                     KRVYFSMNGCGKLITNMGTYTGTDAIEMANGRAVIEFIPVPGEEAVIECRNQDFKGSY
                     LRVKK"
BASE COUNT         1631 a         1028 c          852 g         1665 t
ORIGIN      
        1 tctgctataa atgtgatcac gattgcctta aaaaacaaaa aaagagatgc tggtagacct
       61 taattaatat attgcctggc cttcaatttt tcaccgacga aaaccaggat ttttaacaat
      121 atgtccgata attatctact aaattgttga caatctgggt aacatagcat tataaacaag
      181 tggtctagca taatgttaaa atatatttat gaaagtcatg ttaaatttca ctgaaaatag
      241 cgggaatcac ctttcaagtt cggacattgt aagatgtttc tgctattgaa tattatgcta
      301 acaaatatca tattgaaaaa taaggtatta atcattcgga gcaattaagg tattgataaa
      361 cattcgctcc gggtaatttt tcggctgtaa atcaataaat actttataac cttttgattt
      421 ttctataatc tggaaggcat cataaagttc ctcagtaacg gtataggctt tataatcgag
      481 aatattatta tttatactta ttatttgata gagttgggta ttttcagcgg cacgatccat
      541 ccaacggttt ttagtaatat tatataattt tggtccgctg actgagacaa cgtaaactgg
      601 tccagttgga tcattaggac gaatcggtag gcgctcagga ttattcccac gggcataagt
      661 atggtcgtga ccattgagaa tcaggtcaac cttatacttt ttgaaaattg ggttccagag
      721 atccctaatt tccagaatac cacgaccttc ctttgagcaa taaattggat gatggaaagc
      781 tataattgtc catctattag gattattttt caaaatttct tccagccact gagcctgctc
      841 taatttatac ctagtggaat taagtacaat catacgcaag ccctgaaaat caaaatagta
      901 attggtctcc ggaagttcag tgattccatt taatggtaac gtgaattgtg tccgccaata
      961 aggtgtgaag aggtcctctt catcactttt accactatac tcatggttac caattacggg
     1021 cataatcgga atagtggcat tgatccagct accagacgta aaccattcat tccagccttg
     1081 ataatcgtaa gcactttgaa ccagatcgcc gcagtggatg ataaatctag catcgggact
     1141 ggtcttgtac gctgttcgaa ttgaccgtga ccatagaaaa agtatatttt tttgcgcatc
     1201 acctaagtaa ataaatttaa aaggcatagg ctgtttatca gcggttcgaa attgaaacca
     1261 ctcactccat cgtctttcta aaccaactcg atataaatat aaagtattgg attgtaaatc
     1321 ggtgaaggtc actgaatgat aattagcact tttcccatcg aaatgaagta gttcggtctt
     1381 ggctggtatt cgctgagctt gatagacaaa attgggcgac gcgtcagctc gagctatttc
     1441 tgcataagct gtggaaatgc ttgtgtctgt tcgccaagtt accgactgag tgctgatagg
     1501 atcatcactc caggaaagaa taatatggtc gggatattgt gtcggatcag gataataagc
     1561 cgcctgggat gtatcgctag caattaaaat tgaagtaaat tggcccagaa ttaatatcat
     1621 taaaactaat cccaacagat tgttgcaagc cattgttatt tttgtatttc ttttcatgac
     1681 aaatttactt tcctttttgt taatataatt tactgctttc cagttaaaaa ttagatcata
     1741 ctaagtaaac tctaacagta agataatttg gcaaacatca tacaaagaaa atgattccaa
     1801 ccataaaatg tctactaacg ataatatccc tatgttttta aaaaacttat cgaattcata
     1861 cctgagttat ttatgaaaat actttacgtt ctggatttat atgttatcaa ttttttaatt
     1921 taataatata ataaatcaaa taaaacataa aagttcaaac cgaaaaatcg cttatcatgc
     1981 tcaaattact tgattttcaa taaggaatat ccttcgtgaa cttgtttata aggactgttg
     2041 tgacattact tataaaatac ccgataaaac catcatattt aaaatgaata aaaatacttc
     2101 ttctttaata atttctttcg acataacaga acagtaaatt tctaccattt ttgaattttc
     2161 caaagctttt ccgccaaata gtaaaaatga ttcttcatga cggcgaatac tatttatttg
     2221 ttctaagggc caagtatcag gtctctcgag atagggcaaa atatattgaa ctgattttgc
     2281 taaagaacca cctttggcat tctgaaattt ccataaattg atatgattat tctctgctag
     2341 acgactgaga tatgaccaag cttccaaatt aaaaataaaa taacctaaag attgagttct
     2401 atccatttct aaaggcattt tgccatctgc ttcgatctgc ctagctaaaa gattattaca
     2461 tacataatcc caggtctcat ttgccacaat cttattgcca aggtaattgg cataggtaat
     2521 tacttgaact gcgtaccaag tggaatgatt atttccacgc tgccgctcat ccttaccata
     2581 ttcactatat aatagccaat ctaagtattc actaaaccac ttcgttaatt caatctctaa
     2641 atcaacgtcc caatagccag ccaatttaag aatttgaata gcttcgacaa caaatattaa
     2701 cctacgggta tctaagatcc ctactccccg ttgtttatca ctatttggaa tcacctgagc
     2761 atatttagca tggggagtca ttcttgtcgc tgaatcaacg aaccaaacgt agattagttc
     2821 tttcgccttt tcagcatatt gaatattatc aaacaaatag gctgaggcac ttaaggtcaa
     2881 agttgattga caaagatggt ttaaagcttc tttgtgcttc aagaagcggt tcgggttgcg
     2941 taccccgtct ttcctgatat atggtccatc taaatccttg gggtttggcc accagtaagt
     3001 gctttcacta taataatcgt gacgatcaat attagaatta ggacagggaa agtctacaat
     3061 actgaccgac ttctctttta gtaataaatt agcgctacac attagcgtct ttttataaga
     3121 tagcaattca ccttcaggtt cgactaaagc attttttaac tgctcgagtt ctggccttct
     3181 aaaaaaattt aactgtaact cctcacattg caccgaagcg attagtgcaa ttagcaaaag
     3241 aagtaatttt gattcgaaaa acgaacaaag gctttttagg tgcactattt ttttactctt
     3301 aaataactac ctttaaaatc ctgatttcgg cattcaatta cagcttcttc ccccggcact
     3361 ggaataaatt caattaccgc gcggccatta gccatttcaa ttgcatcggt accggtatag
     3421 gtacccatat tagtaattaa ttttccacac ccattcatag aaaaatagac gcgcttatta
     3481 tagtctagac attgatgact atcttcatca acaaccagcg cctcaatctg taatctgcca
     3541 tttgacaatt gatatgaaga taatgtaatt ctatcaggtt ttccccattt tttcgttgaa
     3601 taattaatta tgatagaatc acggcatgag attccctgag aattatagcc gactgctact
     3661 aatttatttt ggccattctt gaaaatgaca tcccaatata atccacaggc tggaaaatca
     3721 tgaatgttac gttcccttgt tcccaggctg gtactattca ctatcaattc cacttgggag
     3781 gcattactat atacacaaac ttcgcgaggc acattcttcg gaccgttgcg ctcagtccat
     3841 gtatgcgatt cgatatagca aaattctggc gtcttagtcc aatagctctt aaaaacgtaa
     3901 taggcatcct tcggatttcc agcacgatca accaagccct tttggttcat ataaggaatt
     3961 ggattttcca tacgcaatgg tgttgcgaaa tctttaaatg cccactgagc attaccagca
     4021 aaccagtcaa gattctcgga aatatgtaaa taccaatcaa ataaatctac tatataattc
     4081 tcgctccaat caccatattg tgaaatgttc tgcaccttga cctggttaac ttcctcttcc
     4141 caatcttcta gatcgacaaa accttctccg gtaatcggat tttcaacatg acgtcccaga
     4201 tggcttgaac ccccgtattc agcatgaaaa aagtgcttaa attcataacg ggattgatta
     4261 agaatttttt cgtaactttt gtatactcct gaataccaac cagcccaaat tgatggtgaa
     4321 tatacatcta cgatattaga accctcagca aattttcttg tagcagtcaa cctataggga
     4381 tcaagtttat ggactaaatc attcatttca gagaaaaaga ttattaacga atccatgttc
     4441 ccgccattct caaaatcagg caaccagtcc agttcgtttc ctaatgacca aattataatg
     4501 cttgggtgat tataattttg aattatcata tccttaagca tattctttgt gttttgtttc
     4561 catttctcac ctcccatacc accgcggcac catgggactt catcccatac caataaaccc
     4621 agctcatcac aagctcgata aatttccgga tcctgtggat agtgggctaa gcgaacgaaa
     4681 tttgccccga tttccttaat catttgcata tctttacgat gtagttcatt aggaatggca
     4741 ttcccaaacc cagcccaatc ttcatgacga tgagtacctc gcagtaagaa tttctcccca
     4801 ttcaggtaaa aggggccatg ctctttgaat tcaaaccagc ggtaaccaaa tttttcagtc
     4861 atttcatcta caattttacc atcaatattc agactcactt cgatagtgta tagatttggt
     4921 gaaactggag accagagaga aggattagaa atttttggaa atataattgt tgcctcttga
     4981 ttaccggtta cgagtttcaa attttgtttt actgttgcga ctactttacc attaacatca
     5041 attaaatgag ctaaaagctc gccttcaaca gccctatctc tgctattatt taggaggaca
     5101 ttaagactgg tttccgctga ttttttcgaa acttggggaa tagtgattaa tattttctcg
     5161 atatattccg ctggca
//
LOCUS       DAOOMU010000115         5100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_19687_length_5100_cov_23.144301, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000115 DAOOMU010000000
VERSION     DAOOMU010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19687_length_5100_cov_23.144301"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1234)
                     /locus_tag="PLI55_09065"
     CDS             complement(11..1234)
                     /locus_tag="PLI55_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB01019.1"
                     /translation="MNFDEGLKLAFQTVKSNKMRTFLTTLGIVIGVTAVIGMMSIINA
                     LDRYMTKSLSSIGGNVFWIQKYPAVQVGHLDQKYRMRKDLKYEHAEAIKRSATLVSAV
                     SAEFFRWGKTVKYKDRATNPNVLVYGGDEYWADVNGRNISEGRMISLMDVQHRRAVAV
                     LGADVAEKLFPFTYPIGEQVKIDGIRYEVIGVMEKMGQAMMGNQDNIVTIPNSTFIKT
                     YGDWGSINIAVKAKSAETILDAMDQVTAILRIARKVPPGDENDFEVVTADSIMNTLRN
                     LTGFIFIAAVIICGISLLVGGIGIMNIMLVSVTERTREIGIRKAVGAKRRHILTQFMT
                     EAVGICLFGGLIGILLGVVVGLLIAQAFKLPPVIPLWSIFVGMGFSLLIGVVFGTYPA
                     LKAAKLDPIEALRYE"
     gene            complement(1348..2577)
                     /locus_tag="PLI55_09070"
     CDS             complement(1348..2577)
                     /locus_tag="PLI55_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPB01020.1"
                     /translation="MISFFIKLWENVKIALRALMQSKMRTILTTLGIVIGVLTVVSVA
                     SIIEGLNRGFASQIANLGSNALYVERYPWATTEDFSKFRNRPRLTLKEADFIRERMRL
                     AEAVSPSTSTRQDIRYRNRTLNGISVNGVSDHYDVVTGLVIEEGRFFSFAEIERNKNN
                     VVLGWEIKDKLFENRNPIGEKIKIGATTYTVIGVTEKRGSFLGESLDAEVYVPLGTVF
                     KNFGWHRSISIAVKISDPARVEEAKDELRFLMRLARKLTPTQEDNFSINQQEVLTDMY
                     NRLTRGLYTAAIGIGTLSLLVGGIGIMNIMLVSVTERRKEIGIRMALGAKRKVITFQF
                     LVESVIICGVGGIIAIILSFIVSLIISKITPFPSAVPAWSVFMGLGFSAVVGLFFGIY
                     PASKAARLNPIECLRYE"
     gene            complement(2582..3265)
                     /locus_tag="PLI55_09075"
     CDS             complement(2582..3265)
                     /locus_tag="PLI55_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPB01021.1"
                     /translation="MDSLIELKDIVKIYKVGSEEVHALRGVNLSIQPNEYVSIMGPSG
                     SGKSTLMNIIGCLDTPTSGRYELEGVPVHEMDDNQLAEIRNRKIGFVFQTFNLLPRVN
                     ALHNVELPLIYSGIPAAKRKILAEAAIEKVGLSDRKNHKPNELSGGQRQRVAIARALV
                     NNPSIILADEPTGNLDSATGEEIMQIFKDLHEAGNTVVIITHEKDIAERAKRIVHIRD
                     GNIAEDIRN"
     gene            complement(3265..4182)
                     /locus_tag="PLI55_09080"
     CDS             complement(3265..4182)
                     /locus_tag="PLI55_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01022.1"
                     /translation="MKKLFILMFGLAFTTFLSAADSTNTNDPFDELKAIPTQDTLRDE
                     LSEEEEKSLDEIVEDENWKSRLPLNDWNWNWEEDDKEAYRRGQKHGYFRGAAGGWDIF
                     YLPLNLDDLNRSLNPIGVTDFDTYMILNGGGGWVFLGKSIRIGGFGAGGEVIAHGKPG
                     DIQKEVKLSISFGGFTIDKVFHPFSKTELYLGLMIGGGSNTINFYQHGTIVSWDSLIA
                     GYTPNTLTTSHTFHDYSSSLSADYFTLMPTVGFRYNIFRWFAVGGNVGYIYTHIDQKG
                     WRMNHYRVEGVPDLNLDNVIYRLNFYFGG"
     gene            complement(4198..>5100)
                     /locus_tag="PLI55_09085"
     CDS             complement(4198..>5100)
                     /locus_tag="PLI55_09085"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPB01023.1"
                     /translation="KALYERNLAPEADMEAAEAQMELAESQVVQAEAALKQALDDLDK
                     TRITAPMAGIITSVKKEVGEIALGSVFQEDVILVVSDMSKVQVEVDVDETDVVNVSIG
                     DTALIELDAIPNTKFKGVVSEIAHSATTTGVGTQEQVTNFLVKIAVLGQDARFRPGMS
                     ATVDIITDVKEDAIAVPIQALTARPPLVKPPHGEQSPTEKTEKSQYPTPTAVPFGQIK
                     TEEVVFVVKSPVGDTTKTNKPLKVSTGPMAEQRAVKIGISSDTHFEILEGLDEGEEIV
                     VGPYKVISKDIKDGSILKITNKIG"
BASE COUNT         1347 a         1239 c         1077 g         1437 t
ORIGIN      
        1 ctcagcctct ttactcgtac ctgagtgctt cgatcggatc gagtttagcg gctttcagcg
       61 ccggataagt tccgaaaacc acaccaatta aaagcgagaa acccattccg acaaagattg
      121 accagagagg aattaccggc ggcaatttaa atgcctgggc aattaacaat cccactacca
      181 ctcctaatag aattccgatc aagccaccaa acaggcaaat gccaaccgcc tcagtcataa
      241 attgagtaag aatatgacgt cgcttggcac caaccgcctt gcgaatgccg atttcccggg
      301 tacgttctgt caccgaaacc agcataatat tcataatacc gatcccgccg actaacaatg
      361 aaattccgca gataatcacc gccgcaatga atataaaacc ggttagatta cgcagggtat
      421 tcataatcga gtcagctgtt accacctcga aatcattctc gtctccgggc ggaactttgc
      481 gcgcaatccg taatatcgcc gtcacctgat ccattgcatc gagaatcgtc tctgcacttt
      541 tagctttaac ggcaatatta atggaacccc agtcaccgta ggttttgata aaggtcgagt
      601 tcgggattgt gacgatgttg tcctggttac ccatcatcgc ctgacccatt ttctccatca
      661 ctccgatgac ttcatagcga atgccgtcaa ttttgacttg ctcgccgatt ggataggtaa
      721 atggaaataa tttttccgct acatcagctc ctaatacagc tactgcccga cgatgttgca
      781 catccatcag gcttatcatt cgtccttcgc tgatattgcg cccgttgaca tccgcccaat
      841 actcgtcacc gccgtaaact aacacgttgg gattagttgc tctgtcttta tacttgacgg
      901 ttttgcccca gcggaaaaat tccgctgaaa ccgccgaaac cagggtagca ctgcgtttaa
      961 tggcttcggc gtgttcgtat ttcaagtcct tacgcatgcg atatttttga tcgagatgtc
     1021 ccacctgtac ggccggatat ttttgaatcc agaaaacatt acccccaatg ctacttagac
     1081 tcttagtcat atagcggtcc agagcattta taatggacat catcccaata acggccgtaa
     1141 cgccgataac gattcccagc gtcgtcaaga acgtgcgcat cttattggac tttactgtct
     1201 gaaaagcgag ttttaaacct tcgtcaaaat tcatcttgac ctttttccag gaataaaggt
     1261 ggttttaatt tggtggtcgt ttgtgatcat tcagtggtcg ttcgatagtc attcatttaa
     1321 aaatttggta attacggttt tcatgtctta ttcatatctc aggcattcga ttggattgag
     1381 acgggcggct ttgctcgctg ggtaaattcc gaaaaatagt ccgactaccg ctgaaaatcc
     1441 taagcccata aaaaccgacc aggctggaac agccgaggga aacggtgtga ttttgctaat
     1501 gattaatgag acgataaaac tcaaaataat ggcgatgata ccgcctacgc cgcaaatgat
     1561 tactgattcg accagaaatt ggaaggtaat gactttgcgc ttggcgccga gcgccattcg
     1621 gataccgatt tctttgcgac gttccgtgac tgaaaccagc ataatattca taatgccgat
     1681 tccgccgacg agtagtgaca gcgtcccgat cccgatcgcg gcggtataaa gtccacgagt
     1741 caatctattg tacatatcag tcagcacttc ctgttgattg atcgaaaaat tatcttcctg
     1801 ggttggtgtc aatttgcggg caaggcgcat tagaaaacgc aattcgtctt tggcttcttc
     1861 gactctggca ggatcactta tctttaccgc aattgaaata ctacggtgcc agccaaagtt
     1921 tttaaaaacg gtgcccagcg ggacataaac ctcggcatcg agactttcgc ctaagaaact
     1981 accgcgtttc tcggtcacac cgatcacggt ataggtcgtt gcgccgattt taatcttttc
     2041 gccaatgggg ttacgatttt cgaataattt atctttgatt tcccagccta aaacgacatt
     2101 atttttgttg cgttcgattt cggcaaaaga gaaaaaacgc ccctcctcaa tcacaagtcc
     2161 cgtgaccaca tcataatggt ccgagacgcc attgactgaa atgccattta gggttctatt
     2221 gcgataacga atatcctgac gcgtactggt agaaggcgaa accgcttccg ctaatcgcat
     2281 ccgttcccga ataaaatcgg cttcctttaa tgtcagcctc gggcgattgc ggaatttgga
     2341 aaaatcttcc gtagtcgccc agggatatct ctcgacataa agagcattgc taccaagatt
     2401 ggcgatttga ctggcaaatc cgcgattcaa tccttcaatg atcgaagcca ccgaaacgac
     2461 cgtcagaaca ccgattacga ttccgagtgt cgtcagaata gtgcgcattt tgctttgcat
     2521 aagcgcacgc aaagcaattt tgacattttc ccagagttta ataaaaaagg aaatcacgtt
     2581 ttcaattccg aatatcttca gcgatattgc cgtcgcgaat atggacaatc cgcttggctc
     2641 tttcggcaat gtctttttcg tgagttatga ttacgacggt attgccagcc tcatgcaaat
     2701 ccttaaaaat ctgcatgatt tcttcgccgg tggccgaatc cagattgcca gttggttcat
     2761 cggcaagaat aatagacgga ttattaacca atgctcgagc gattgccacg cgctgacgtt
     2821 gaccgccgga tagttcattc ggtttgtgat ttttgcgatc agaaagacca actttctcaa
     2881 tggcggcttc ggctaatatt ttccttttag ccgccggtat gccactgtaa atcaatggca
     2941 gttccacgtt atggagggcg tttacccgcg gtagtaaatt aaaagtctga aacacgaagc
     3001 caattttgcg attgcggatt tcagccagct gattgtcgtc catttcgtga actggtacgc
     3061 cttctaattc gtagcgaccg gaggtcggag tatccagaca gccgataata ttcatcaagg
     3121 tggatttacc ggaaccggat ggtcccataa tcgagacata ttcgttcggt tggattgaga
     3181 gattcacacc gcgtaacgca tgaacctctt cgctaccgac tttatatatt ttaacaatat
     3241 cttttaattc aattaaagaa tccattagcc accgaaatag aaatttaaac gatatattac
     3301 attatcaagg ttcagatccg gcacaccttc aacgcgatag tgattcatcc gccaaccttt
     3361 ttgatctata tgggtgtaga tatatccgac atttccacct actgcaaacc agcggaagat
     3421 attatagcga aaacctaccg taggcataag ggtgaaataa tcggctgaaa gagaactgct
     3481 atagtcatga aaagtatgag atgtcgttag agtattggga gtatagccgg caattagact
     3541 atcccaagaa acaatcgttc catgttgata aaagtttatg gtattactgc caccaccaat
     3601 catcaaaccg agatacagct ccgttttact aaaggggtga aaaactttgt caattgtaaa
     3661 acctccaaaa ctaattgaaa gtttaacttc tttctgaata tcacccggtt tgccatgggc
     3721 aatcacctct cctccggcac caaatccccc aattcggata ctttttccta aaaataccca
     3781 gccgccacca ccgttcagaa tcatatatgt atcaaagtca gtgacgccga tcgggttaag
     3841 agagcgattc aaatcatcta aattcaacgg caggtaaaaa atatcccaac cgccagccgc
     3901 gccacggaaa tagccgtgtt tctgacccct tctataggct tctttgtcat cctcttccca
     3961 attccaattc caatcgttga gcgggagacg acttttccaa ttttcatcct cgacgatttc
     4021 atccagcgat ttttcttctt cttcagaaag ttcatcgcgg agggtatcct gggtgggaat
     4081 tgctttcaat tcatcaaagg gatcattggt attggtcgaa tcagccgcgc ttaaaaacgt
     4141 agtaaatgcc aatccaaaca ttagaatgaa taactttttc attgctttgc tccttcttta
     4201 tcctattttg tttgtgattt ttaatatcga accgtctttg atatctttgc tgatgacttt
     4261 ataggggccg acaacgattt cctcgccttc atcaagacct tcgaggattt caaaatgggt
     4321 gtcggagcta atgccgattt taacagcgcg ttgttcagcc attgggccgg ttgatacttt
     4381 taaaggttta ttagtcttgg ttgtatctcc gactggagat ttcacgacga acacgacttc
     4441 ctcagtcttg atttgtccga agggaaccgc agtcggtgtc ggatattgtg atttttctgt
     4501 tttttcagtt ggggattgtt cgccatgtgg cggttttaca agcggcggtc gcgccgtaag
     4561 cgcttggatc ggaactgcaa tcgcatcttc ttttacatcg gtaataatat ccacggtggc
     4621 cgacataccg ggtcggaagc gagcatcttg accgagaacg gcaatcttaa ccagaaaatt
     4681 ggttacttgc tcctgggtac cgactccggt cgtagtggcg gagtgggcaa tttccgatac
     4741 gactccctta aatttcgtat tggggatagc atccagttcg attagtgccg tatcgccgat
     4801 tgatacattg acaacgtcag tttcatccac atccacttcc acctgaactt tggacatatc
     4861 cgaaacgacg aggattacgt cttcctgaaa gaccgagccg agggcgattt cgccgacttc
     4921 ttttttcacg ctggtaatga ttccagccat tggagctgtg atacgggttt tgtcgagatc
     4981 gtccagtgcc tgcttcagag cggcttccgc ctggacaacc tggctctccg ccaattccat
     5041 ctgagcttca gccgcttcca tatctgcttc cggcgctaaa ttcctttcat agagagcctt
//
LOCUS       DAOOMU010000116         5059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_19893_length_5059_cov_16.363709, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000116 DAOOMU010000000
VERSION     DAOOMU010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19893_length_5059_cov_16.363709"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..1009)
                     /locus_tag="PLI55_09090"
     CDS             complement(176..1009)
                     /locus_tag="PLI55_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01024.1"
                     /translation="MVKRIILLLLAIFSVIFSTENLGDYYNAKQHYFEAITEYKRQLF
                     FGQYESQDEILLKMAKVYHAVQQDLFAEEILLQAISNDEASAFDWECSLELARLHWDN
                     YNYFEMRNVLDFISINSEQPIQDTINYIKAWTYFYEAKWGEGLALLDSVDFIDSKSLQ
                     KEISGVHKIPQKSRTLALVMSNIIPGTGHLYAHDYQNALYSSLLVGGTMVSIISNVID
                     RAYFVALIKYLFIYSRYNNGTLVNLAKKVDRDNIDAIGNYLKTVSQKYPAPLELLECL
                     R"
     gene            complement(1003..1839)
                     /locus_tag="PLI55_09095"
     CDS             complement(1003..1839)
                     /locus_tag="PLI55_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01025.1"
                     /translation="MKKSILFLLILSISTTANAQIESSLFEPAHLKEFGLYLFQQKDY
                     LRAAMELERYRYFAKSGDDSITFVIGLCHQFRQRYDFAAATFRELAEDDSSKFKESAR
                     LAMLYNYTKLEDWSKILGLSYQNDAEFYFYYLASVKTAPTTITTSFFQNVNDESLRNQ
                     LKALEQDRNKIHPKSPFLAGTLSTLIPGLGKVYINRSGDALFSFGMINLFALLTYKAF
                     QAELPVTGVLSGGLTLSLYLGTIYGSYVGAELYNRNLDADWQSKMTELNPVDRHPYWS
                     TW"
     gene            complement(1852..2193)
                     /gene="yidD"
                     /locus_tag="PLI55_09100"
     CDS             complement(1852..2193)
                     /gene="yidD"
                     /locus_tag="PLI55_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPB01026.1"
                     /translation="MLSQEHQKWTDYWEIPLNLPKEPGEIFSTNLFSSSAIAWIKIYQ
                     KVIASQDLPSCVFHPSCSRFALTSIQEFGIVKGALLASDRLMRCNPFAMRYYPFDGEK
                     LADPPQKYRLK"
     gene            complement(2329..2772)
                     /locus_tag="PLI55_09105"
     CDS             complement(2329..2772)
                     /locus_tag="PLI55_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01027.1"
                     /translation="MLKKKNTILIFWLLASFAFAEEKGGVMPCLASFFIGPRVGLEMN
                     EGKQIETIEWIHFGSNFVPYVGLVVKAYTSYEIGGKTNGAGGFFASCCIGPRVGAELN
                     YRKIRTLEWLRLVPCINIYPLIKIPLEAYQGKTMTEIEQKEGLRK"
     gene            3133..5013
                     /gene="ftsH"
                     /locus_tag="PLI55_09110"
     CDS             3133..5013
                     /gene="ftsH"
                     /locus_tag="PLI55_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPB01028.1"
                     /translation="MKKNEKFSLIYFWLITLMIIFTQLLFFPDRPANVRLSYRDFLNA
                     LDSGKVARIVIFEDRIVGEFKSEDINEEQALTHAPTAPWRLRVPSIEKQVARQFIVSR
                     IPELPDPELLAKLQASGVNFEGRYESNSLRNFFLNWVFPIIFFIGLWGVIFRRMGSSN
                     PMLDFGRNKAKIYAEKPENQVKFSDVAGVDEAVEEVKEVVDFLKNPKKYTRLGGKLPK
                     GLLLVGPPGTGKTLLARAVAGEAGVPFFNLSGSDFVEMFVGLGAARVRDLFKQAKAAA
                     PCIIFIDEIDAIGKSRGNALTYGGGYDERENTLNQLLVEMDGFDPGVGVIIIGATNRP
                     EVLDPALLRPGRFDRQVLVDRPDMKGREAIFKVHTRNLKLSSNVDLKQLAAQTPGFAG
                     AEIANVCNEAALLAARNNRDEVTQSDFEAAIERLIAGLEKKNKIINEKERKIVAYHES
                     GHAIVGYFTPGAEQVQKVSIVPRGIGALGYTLQMPLEDRYLMTKSEIIGKIKGLLGGR
                     AAEEIVFNEISTGASNDLERVAQLVKNMIVVYGMSEKLPNYSLVNRSTPGFLNQSAGI
                     ERRSEHLERLIDEEVKEIIDKCYRETKEMLIEKRELLDKMANTLLEKEVLEKEEIEAI
                     LS"
BASE COUNT         1588 a          961 c         1054 g         1456 t
ORIGIN      
        1 gtgacagtat ggctcagagt gacagtatgg ctttttttct cagcctcagt ctcttttctc
       61 agcctcttta ctaatgccca atgaccaatg accaatgacc aataaccaat gactactctc
      121 agcctcaacc ttattaatcg tgctttagat cctttgtggt taaaaggctt ttatatcatc
      181 gcaaacactc cagcaattcc agaggcgctg gatatttctg cgagacagtt tttagataat
      241 taccaatcgc atcaatatta tctctatcta cttttttggc gaggttaacc aatgttccat
      301 tattataacg ggaatagata aatagatatt taatcaaggc aacaaaatag gctcgatcga
      361 tgacattcga aatgatcgaa accatcgttc cgccaaccaa aagagaagag taaagggcat
      421 tttggtaatc gtgcgcgtac aaatgcccgg tgccgggaat gatatttgac attaccagcg
      481 ccagagtgcg cgatttttga ggaattttat gaacaccgga aatttctttc tgcaatgatt
      541 ttgagtcaat aaaatcgacc gaatctagca aagccagacc ttcgccccac ttggcttcat
      601 aaaagtaagt ccaggctttg atataattga ttgtatcctg aattggttgc tctgaattta
      661 tactaataaa atccaagaca ttgcgcattt cgaaatagtt ataattatcc caatgcagac
      721 gagctaattc gagtgaacat tcccagtcaa aagcagacgc ctcatcattg gagatggctt
      781 gtaaaagtat ttcttcggcg aataaatcct gctgaacggc gtggtatacc tttgccattt
      841 ttagtaaaat ttcatcttgg ctttcgtact ggccaaaaaa tagttgccgt ttgtactcag
      901 taatggcttc gaaataatgt tgtttggcat tatagtaatc gccgagattt tccgtagaaa
      961 aaattaccga gaagatcgca agtagtaaaa gaattattct ttttaccatg tggaccaata
     1021 agggtgccga tcaaccggat tcagttcagt cattttactt tgccaatcgg catctaagtt
     1081 acgattataa agttcagccc cgacataact gccgtagatt gttccgagat aaagcgagag
     1141 agtcaatcca cctgacagaa cgccagttac aggcagttcg gcttgaaagg ctttataagt
     1201 caaaagcgca aagagattga tcattccgaa cgaaaagagc gcatcgccgg accgattgat
     1261 gtaaactttt cctaatccgg ggataagagt tgataatgtt ccggctaaaa aaggtgattt
     1321 ggggtgaatt ttattacgat cctgttcaag tgctttaagt tgattgcgca gactttcatc
     1381 attaacattt tgaaaaaagg aagtggtaat tgtagttgga gctgttttca cgctggctaa
     1441 ataatagaaa taaaactcgg catcattttg ataacttaat cccaggattt tagaccaatc
     1501 ttctaatttt gtataattat ataacatagc taagcgcgca gattctttaa atttactgga
     1561 atcatcctca gccagttccc gaaaagttgc ggcggcaaaa tcataacgct ggcggaattg
     1621 atgacatagc ccaataacaa aagtaatcga atcatcgcca ctttttgcaa aatagcgata
     1681 acgttcgagc tccatcgccg ctcgcaggta atctttttgc tgaaacaggt aaagtccaaa
     1741 ttcttttaag tgtgccggtt caaaaagaga agattctatc tgagcattgg cagttgttga
     1801 aatcgaaaga atgagaagaa ataaaataga ttttttcaca atggctctgg tctattttaa
     1861 acgatatttt tgaggtggat cggctaattt ttcgccgtca aatggataat agcgcatagc
     1921 aaatgggtta cagcgcatca agcgatcact ggctaataaa gcgcccttga caatgccgaa
     1981 ttcctgaatg gaggtcaagg cgaaacgtga acaagaaggg tggaagacgc acgatggtaa
     2041 atcctgactg gcaatcactt tttggtaaat tttaatccag gctatagccg aagaagaaaa
     2101 taagttggta gaaaatattt ccccgggttc ttttggtaaa tttaaaggta tttcccagta
     2161 gtctgtccat ttttggtgtt cttgggaaag caacaaattc caaaataata agatggaaaa
     2221 aattaccaat tttattttta tctttttacc catcgaattg gatttaaaaa gaaagctgaa
     2281 attaaaccaa ctttaattcc agctttcaat tcataaaaaa atctgaattt acttgcgaag
     2341 tccttctttt tgttcaattt cggtcattgt tttgccctga taggcttcca gtggtatttt
     2401 gatgagtgga taaatgttaa tacacggaac taatcttaac cattcgagag ttcttatttt
     2461 tcggtaattt agctcagccc caactcgcgg accgatacaa caacttgcaa aaaaccctcc
     2521 tgctccatta gtttttcccc caatttcata acttgtataa gcctttacaa ctagaccgac
     2581 atatgggaca aaattactgc caaaatgaat ccattcgatt gtctcaatct gcttaccttc
     2641 gttcatttcc aaacctacac gcgggccaat aaaaaaactt gctaaacaag gcattacccc
     2701 gcctttttcc tcggcaaatg caaaactcgc caagagccaa aaaatcaaaa tggtgttctt
     2761 ctttttcaac ataaattgtc ctccatgttg tcgattctcg ttttaaaagt gataaattat
     2821 agtgagttat ttctggaaat gcaagaattt tcgcgagtca aaaaagatgt ttagtttaaa
     2881 attactggca ttttcagcat attcgtaaga atttgatatt taataatttt caaagtaatt
     2941 tcaagaaaat gcggtttgat tcaagcctta taacacctac tgaaaaaatt aaatatgttt
     3001 tcaggctgac ataactcctt aatcttatga atcctgagtt accgagagaa attataatgg
     3061 caaaataagc ggaaatgatt taaatttttc ataaatgaag tgtcaattga aaactgatga
     3121 ggtgagacag ttatgaagaa aaatgaaaaa ttttcactga tttacttctg gttaatcaca
     3181 ctgatgataa tttttaccca gttgttattt tttccggata gaccggctaa tgtccgcttg
     3241 agctatagag actttttaaa tgctttggat tccggaaaag tagcgcgaat cgtaattttc
     3301 gaggatcgca tcgtaggcga attcaaatcc gaagatatta atgaagagca agcgcttact
     3361 catgccccga ccgcaccgtg gcgattgaga gtgccatcga ttgaaaaaca ggtagcccgg
     3421 caatttatcg tcagccgtat tccggaactt ccagatccgg aactgttggc gaaattacaa
     3481 gcctcgggtg ttaattttga aggtaggtat gagtcgaact cgcttcgtaa tttttttctg
     3541 aactgggtat ttcctattat cttttttatc ggtttatggg gggtaatttt cagacgaatg
     3601 ggaagtagca acccgatgct cgatttcggc cggaataaag ctaaaatata tgccgaaaag
     3661 ccggagaatc aggtcaaatt ttccgacgtg gctggcgttg atgaagccgt tgaagaagta
     3721 aaagaagttg tagatttttt aaagaatcct aaaaaataca caagattagg gggcaaatta
     3781 ccgaagggct tattgctagt tggaccgccc gggactggca agactttgct agccagagcc
     3841 gtggcgggag aagccggagt gccatttttt aacctcagcg gttcagattt tgtggaaatg
     3901 tttgtcggct taggcgcggc tcgggtcaga gatttattca agcaagccaa agccgccgct
     3961 ccctgcatta ttttcattga tgaaatagat gctatcggga aaagccgtgg taatgctctg
     4021 acttacggag gcggctacga cgaacgtgaa aatacgctca accagttact ggttgaaatg
     4081 gacggtttcg atcccggagt aggggtaata attatcggag ctactaacag accggaagtg
     4141 cttgaccccg ctctcctaag acccggacga ttcgatcggc aggtactggt tgaccgtccc
     4201 gatatgaaag gtcgagaagc gatatttaaa gtgcatactc gtaatctaaa actcagctcg
     4261 aatgttgatt taaagcaatt agccgcccaa acgcctggat ttgccggtgc tgaaattgcc
     4321 aatgtatgta acgaggccgc tcttttagcc gcccgaaaca atcgcgatga agttacccaa
     4381 tctgattttg aagccgctat cgaacgtctt attgccggtt tggaaaagaa aaataaaatt
     4441 atcaacgaaa aagaacgtaa aatcgtcgcc tatcatgaat cgggtcatgc gatcgtagga
     4501 tattttactc caggcgccga acaggtgcaa aaagtatcca tagtgccgag aggaataggt
     4561 gcgctcggtt atactttgca aatgccactc gaagatcgtt acctgatgac taaatctgaa
     4621 attattggaa aaataaaggg attactcggc gggcgtgccg ctgaagaaat cgtctttaat
     4681 gagatctcga ctggcgcttc caatgatttg gaacgcgtgg ctcaattagt gaaaaatatg
     4741 atcgttgtgt atggtatgag cgaaaaactg ccgaattatt ctctagtcaa ccgttcaaca
     4801 ccgggatttc tcaaccaatc agccggaatt gaaaggcgct cagaacatct cgaaagattg
     4861 attgacgaag aagtcaaaga aattattgat aaatgctacc gtgaaaccaa agagatgtta
     4921 attgagaaaa gggaattact ggataaaatg gcaaatacgc ttttagaaaa agaagtgtta
     4981 gaaaaagagg aaattgaggc gattttatcc tgattaattc ggataataga taatcataga
     5041 gattaagaaa gaacttaag
//
LOCUS       DAOOMU010000117         5038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_20010_length_5038_cov_17.345374, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000117 DAOOMU010000000
VERSION     DAOOMU010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20010_length_5038_cov_17.345374"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="PLI55_09115"
     CDS             complement(<1..694)
                     /locus_tag="PLI55_09115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPB01029.1"
                     /translation="MLKLKQIGIITVLILMNTVHAGEINPSYEVGIWQGFRSAAVTFT
                     FDDGCANQFTVAIPLFDQHGFKLTLYPVINWSGGIWSKLQSAAANGHEVGSHTVTHPD
                     LSGLSEAEQITELQNSQNEINSRISGQRCITIAYPYCNAGKTSICKQYYIAARGCSGQ
                     IEPKTPSNFMNISSIVCGTEGSVKTANDFFNKAKSAANSKGWVVFLIHGIDNDGGWSP
                     VTTENLTGAVDSL"
     gene            complement(817..2979)
                     /locus_tag="PLI55_09120"
     CDS             complement(817..2979)
                     /locus_tag="PLI55_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65"
                     /protein_id="HPB01030.1"
                     /translation="MGKNRQKYDKKRNLNELLAIVVFLSSFVVFFSCENNAKIDREAL
                     VKRHSPTLNASDPLNPFTVGNGEFAFSTDFTGLQSFPECYEETIPLVTQAQWGWHSFP
                     NLNQYSLDQVYEEYEVNGRKVAYASHQDSEAGEYLRSNPHRLHLGQIGFKFKDQDGLP
                     IGVDEIRNIKQTENIWEGIIYSSFEVLNTMMEVETACHPVYDQIAVRIKSALLNQGKV
                     AISFNFPYASSRWGKNPADWYSPEKHISAIVSESPNSVLIKRTLDTTEYYVKIYWRGD
                     AEFTKTDRHSFNLAIKSGKNFEFTCLFLERESGQGSASVTQTFSAARSFWQNFWETGG
                     AIDLSASKDSRAKELERRIILSRYLTRIQCAGSLPPQETGLTCNSWYGKFHLEMHWWH
                     AVHFVLWQKPELFEKSLPFYQRILPMAIRTAERQGYAGARWPKMVGPDGEESPSSIGV
                     FLIWQQPHPIYYAELLYRYHQDPVILEKYKDIVFSSAEFMASFVQWDAKNGCYILGAP
                     LIPAQEIYPPDITLNPGFELSYWRFGLETAQKWRERLGLKREEKWDTILELLAPLPQN
                     NGYYQNAENAMNTFENSSHRKDHPTLLASYGMLADKTVDQKVMEKTLEKVMQSWDWET
                     TWGWDFPLIAMTAARVGRADLAIEALLMDVPKNTYLLNGHNYQNESLPVYLPGNGSLL
                     TAVAMMAAGWDGAPDIYAPGFPQDGQWSIKYENLVPLP"
     gene            complement(3182..>5038)
                     /locus_tag="PLI55_09125"
     CDS             complement(3182..>5038)
                     /locus_tag="PLI55_09125"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HPB01031.1"
                     /translation="FSVLLLVLLALTVNAQNFSITIDAEKDAFYSELTGPSNGLVFMP
                     YLCYLRDIGTPPTDNADLSAKVWFSYDADYLYCYAEVKDDIVAATNTERFQNDCMELK
                     FDPDPNSGTNTKTANCRITAKGADNAEEPTGVDNLNGSGHLEDVEGTDYVVSESDYAR
                     RLTDDGYVLEFRVPFDYINEPEDNRFMVERTVGNKFGMAINIGDNDGTTREHMIQWSA
                     GHTDGAHSYPALCGTVTFMDNHVLKLEAVGPRPQDTVVVNPNAGWYIDPNFAVAIDAE
                     KDAFYSELTGPSNGLVFMPYLCYLRDIGTPPTDNADLSAKVWFSYDADYLYCYAEVKD
                     DIVAATNTERFQNDCMELKFDPDPNSGTNTKTANCRITAKGADNAEEPTGVDNLNGSG
                     HLEDVEGTDYVVSESDYARRLTDDGYVLEFRVPFDYINEPEDNRFMVERTVGNKFGMA
                     INIGDNDGTTREHMIQWSAGHTDGAHSYPALCGTVTFMDNHVLKLEAVGPRPQDTVVV
                     NPNAEEWYTKDPTVAIKVEPSVAKSFNLLTNYPNPFNPSTTIKFSVPKTAFVTLKVHN
                     TLGQEVAQLVAQRLNEGVYTVNWNAAEFPSGIYIVRMVAGDLINTSKMILLK"
BASE COUNT         1446 a         1199 c          952 g         1441 t
ORIGIN      
        1 ttagagaatc aaccgcacca gtcagatttt ccgttgtcac tggcgaccaa ccgccgtcgt
       61 tatcaatgcc atgaattaga aaaacgaccc agcccttgga gttcgccgcg cttttggctt
      121 tattaaagaa atcgttagcg gtttttactg aaccttcagt accgcatacg atagaactga
      181 tattcataaa attactcgga gttttcggct caatttgccc ggagcaaccg cgggcggcga
      241 tgtagtattg cttgcaaatc gaagttttac cggcattaca gtagggataa gctattgtga
      301 tgcatcgctg tccgctgatg cgagagttaa tttcattttg cgaattttgt aattctgtga
      361 tttgttccgc ctccgataaa ccgcttaaat cagggtgagt tacggtgtga ctgccgactt
      421 catggccgtt tgccgcggcg gattgtagtt ttgaccaaat tccacccgac cagtttatta
      481 ccggatataa cgtgagtttg aatccatgct ggtcaaagag tggaatagcc acggtaaact
      541 ggttggcgca accatcatca aaggtaaaag tgaccgccgc ggaacgaaat ccctgccaga
      601 ttccgacttc atagctcgga tttatttccc cggcgtggac cgtgttcatt aaaattaata
      661 ctgtgatgat accaatttgt ttgagcttca acataaatta ctcctgtatt aaataaggct
      721 aaatttcaaa tttttgaacc gagaaatatt atttattttt ttatccttaa tgcaaatttt
      781 atttaaccta cttatcttag aaattctcat aatgttttat ggtaaaggca ctaagttttc
      841 atatttaata gaccattgcc catcttgcgg aaatccagga gcataaatat cgggagcacc
      901 gtcccatccg gccgccatca tcgccacggc cgtcaataaa cttccgtttc caggcaaata
      961 aaccggtaag gattcatttt gataattgtg accattcaaa aggtaagtat ttttgggtac
     1021 atccatcagt aaagcctcga tcgccaaatc cgctctgcct acccgagccg ccgtcattgc
     1081 tatcaaggga aaatcccagc cccaagtcgt ttcccaatcc caggattgca taaccttttc
     1141 aagcgttttt tccataacct tttgatcaac agtcttatcg gctaacattc cataggaagc
     1201 caataaggtt gggtgatctt tcctatgact actgttctca aaagtattca tcgcattttc
     1261 ggcgttttga taataaccat tattttgggg aagcggcgct aataattcga gaatagtgtc
     1321 ccatttttct tcccttttca atcctaaccg ttctctccat ttttgggcgg tttccaatcc
     1381 aaaacgccaa tatgacagct caaaaccagg attaagtgtg atatccggtg gatagatttc
     1441 ctgagcgggg attaacggtg ccccgagaat atagcagccg ttttttgcgt cccattggac
     1501 gaaagatgcc ataaattcag ctgaactgaa tactatgtct ttatatttct caagaataac
     1561 cggatcttga tgataacgat acaataactc cgcataataa attgggtgag gttgttgcca
     1621 gattaaaaat actccgatgc tcgacggact ttcttctcca tcgggtccaa ccattttcgg
     1681 ccagcgtgct ccggcatacc cttgccgctc ggcagtacga atcgccattg gcaatattct
     1741 ttgataaaaa ggcaaactct tctcaaaaag ttccggtttc tgccaaagca caaaatggac
     1801 ggcatgccac cagtgcattt ccaaatgaaa tttcccatac cagctgttgc aggtcaaacc
     1861 ggtctcctga ggtggcaaac ttccggcgca ttggatacga gtcaaataac gtgataggat
     1921 aattctgcgc tctaattcct ttgcccgtga atctttactt gccgaaagat ctatagctcc
     1981 gccggtttcc cagaaatttt gccaaaatga tcgtgcggcg gaaaaagtct gggtaacgct
     2041 tgcggatccc tggccacttt ctctttctaa aaacaggcaa gtgaattcaa aatttttacc
     2101 ggatttgatt gccagattaa acgaatgtcg gtcggttttt gtaaactcag catctcctcg
     2161 ccagtaaatt ttgacatagt actccgtggt atctaaagtc ctcttgatta aaactgaatt
     2221 agggctttcc gaaacgatgg cggaaatatg tttttcgggg ctgtaccaat cggctggatt
     2281 tttcccccat ctcgacgaag cgtagggaaa attaaaactt attgcaactt ttccttgatt
     2341 caacaatgcc gacttgattc tgaccgcaat ttgatcatat accggatggc aggcggtctc
     2401 tacttccatc atagtattca agacttcaaa ggaactgtaa attatcccct cccagatatt
     2461 ttcggtttgc ttgatgttgc ggatttcatc tacaccaatc ggtaaaccat cctgatcttt
     2521 gaacttgaaa ccaatttgtc ctaagtgaag gcgatgcgga tttgagcgta aatattctcc
     2581 tgcttctgaa tcttggtgcg aggcataggc tactttccga ccgttaactt catattcttc
     2641 ataaacttga tccagcgaat attggtttaa attaggaaaa gaatgccagc cccactgcgc
     2701 ctgagtcact aacgggatgg tctcttcata acattctgga aaactttgta atccggtaaa
     2761 atcggtagaa aaggcaaatt caccgtttcc taccgtaaat ggattcagag gatcgcttgc
     2821 gttcaaagtg ggactatgac gtttgaccaa cgcctcgcgg tcaattttcg cattattttc
     2881 acaggaaaaa aatacgacga aagaacttaa gaaaacgaca attgccaata attcgttcag
     2941 gtttcgtttt ttatcgtatt tttgtctatt ttttcccatt acaaatgcca tcaaaaacaa
     3001 gaggttgtct taaaagcccc attgtgtttt cccctttttg gtcattgcta tcttaacgac
     3061 cccgcgcctg cttagccaaa atctctactt cgtcaaaaat ccaggataca atttccacaa
     3121 aggcgctatg aaacacaaag taaaaaaact aataagacaa cctctaattt tttgcgtcgg
     3181 tttacttaag caaaatcatc ttgcttgtat tgattaaatc acccgcaacc atccgaacga
     3241 tataaattcc actcggaaat tcagcggcat tccaattaac ggtatagaca ccttcattca
     3301 atctctgcgc taccaattga gcgacttctt gacctaaggt attatggact ttcaaagtga
     3361 caaaagccgt cttaggaacg gaaaacttaa tcgtagtgct tggattaaat ggattcggat
     3421 aattagttaa aagattaaaa gacttggcta cagacggttc aactttaata gctacagttg
     3481 gatctttcgt gtaccattct tcggcattgg gattcactac cacggtatcc tgaggacgtg
     3541 gtccaacagc ttctaacttc aatacatgat tatccataaa tgtcaccgtt ccacaaagtg
     3601 ccggataaga atgagctcca tcggtatgcc cagctgacca ctgaatcata tgttcacggg
     3661 tagtcccatc attatcacca atattgatcg ccatcccaaa cttattccct accgtgcgct
     3721 ctaccataaa ccgattatct tccggctcat tgatgtaatc aaacggcacc cgaaattcaa
     3781 gaacatagcc atcatcagtc aatctacggg cataatccga ttccgaaaca acataatcgg
     3841 taccttcaac atcttccaga tgaccagaac cgttcaaatt atcaactcca gttggttctt
     3901 cagcattatc ggcaccttta gcagttatcc gacaattcgc ggtcttcgta ttggtaccac
     3961 tgttcggatc aggatcaaat tttaactcca tacagtcatt ctggaacctc tctgtattgg
     4021 tagccgctac gatatcatct tttacctcag cataacagta gagataatcg gcatcgtaag
     4081 aaaaccagac tttagccgag agatcagcat tgtcagtcgg tggcgtccca atatcccgca
     4141 gatagcataa ataaggcata aacaccaacc cgttcgacgg accggttagt tctgaataaa
     4201 atgcgtcctt ctcagcatca atcgcaactg cgaaattagg atcaatatac caaccggcat
     4261 tgggattcac taccacggta tcctgaggac gtggtccaac agcttctaac ttcaatacat
     4321 gattatccat aaatgtcacc gttccacaaa gtgccggata agaatgagct ccatcggtat
     4381 gcccagctga ccactgaatc atatgttcac gggtagtccc atcattatca ccaatattga
     4441 tcgccatccc aaacttattc cctaccgtgc gctctaccat aaaccgatta tcttccggct
     4501 cattgatgta atcaaacggc acccgaaatt caagaacata gccatcatca gtcaatctac
     4561 gggcataatc cgattccgaa acaacataat cggtaccttc aacatcttcc agatgaccag
     4621 aaccgttcaa attatcaact ccagttggtt cttcagcatt atcggcacct ttagcagtta
     4681 tccgacaatt cgcggtcttc gtattggtac cactgttcgg atcaggatca aattttaact
     4741 ccatacagtc attctggaac ctctctgtat tggtagccgc tacgatatca tcttttacct
     4801 cagcataaca gtagagataa tcggcatcgt aagaaaacca gactttagcc gagagatcag
     4861 cattgtcagt cggtggcgtc ccaatatccc gcagatagca taaataaggc ataaacacca
     4921 acccgttcga cggaccagtt agttctgaat aaaatgcgtc cttctcagca tcaatcgtaa
     4981 tggaaaaatt ctgagcattt acggtcaatg ctaacaaaac aagaagcaaa accgaaaa
//
LOCUS       DAOOMU010000118         5018 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_20119_length_5018_cov_16.786420, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000118 DAOOMU010000000
VERSION     DAOOMU010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20119_length_5018_cov_16.786420"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLI55_09130"
     CDS             <1..573
                     /locus_tag="PLI55_09130"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPB01032.1"
                     /translation="LGYAKRLTEWFSVGGQIRLVKQDLGQSVIPKISTIPDTTEYVVK
                     NTLTPLAFDFGTQFKTGFKSLVFGMSIRNFSTDIKYVEEPFQLPLVFNLGISMDLFDL
                     ISQINQNQSLYMSIDASHYRSRAEQVKIGLDWQLLKVLSLRCGYITNEDESGLTFGIG
                     VSKFGFTFDYAYTPYGVFNQVQRLTVRMSF"
     gene            596..1786
                     /locus_tag="PLI55_09135"
     CDS             596..1786
                     /locus_tag="PLI55_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01033.1"
                     /translation="MKSNRFLIISALCLAVMKCEYDVTQPQWYQDYTEPATPAITNIE
                     PDSAVAGCNYINIIGENFSGTPTVYFDNVTAEVISNLGNSIQVRRPNLASDGCLVKVA
                     CDSALVVAQSTEPYKITQVVQKYGNFVENKELNAVAVDSVENVYVIDNTNIIYKVTPA
                     GDKTTMPDKAKRTTYDAIIGPDGKLYLLGNNRSIDVLDPQSGKVAEWIKLKAGKVVKF
                     GDFDKNGYLYTGGRSTGLVVVAPDLTPNYTSLYEKDEIKGIRVFGNYVYVSLVCTTPD
                     LQNPALAIWRNPIDNSGNPGEKELVLDLTAYKNYSADALNSFSLSSDGKLFLGINAQT
                     PLLAIDLNTTSADYFYLGIIAPYCKSFSWGWGNYLYMICGDTALGVEWTLYRIDMGVT
                     SARE"
     gene            complement(1879..4668)
                     /locus_tag="PLI55_09140"
     CDS             complement(1879..4668)
                     /locus_tag="PLI55_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPB01034.1"
                     /translation="MKSQITYSLIILALAVSQCGQSGKVKPIQNGVDLKTRDLNVRVQ
                     FYADDIARVIKWRPESSAEKLSLMVIRDSLPDLSITVEENDNDVILKSERMTIRLVKK
                     DGHLEYFTTSGTSILKENKKAVFTPVTLPYEKAFNIQQNFKLTPDEGLYGLGQHQDGY
                     MNYRGRTVKLVQTNTDAVTPFLISTRGWGILWDNYSKTIFQDTAETMSLWSEVGDNLD
                     YYLIYGGTMDSVISGYRYLTGKAPLYGKWAYGYWQSKEHYKNRAELLSVVSEYRLRRI
                     PIDNIIQDWNYWDGNENWSQMFFDPKKYPEPEEMIRQIHNQNFHIMISIWPGLGPNTA
                     IYQEMEQNGFLYNTVGWANFKYFDAFNPAANDLYWKYIKNGLISVGIDALWIDSTEPD
                     IVNATTKESQEYEMKKVGRNYLGSWARYLNAYSLVMMDKLYQNQRQDSDRKRVYMLTR
                     SVFAGQQRTGATTWSGDIGASWEIYQNQISAGLNFCMAGIPYWTFDIGGFVIGAYGGV
                     FINGGQDPAYQELYTRMFQFGTFCPIFRAHGSETPREIWCFGEFSPHLIKFDHLRYRL
                     MPYIYSLAWRVTNDDYTMMRGLPMDFPNDPETYSLGDQYMFGPAIMVCPVTEYMLHRP
                     PGKSILISGDHFKTNDGKAGLKVQYFRDPDFRILSHEQIEPDINHLWYTGRPDYMTDS
                     AFSVRWEGKLIPSQTGSHQFHLKSYDAKRIFINGKELPIVYTSVEQYTDLINLKAGQG
                     YDFRLEMQNRSTGAAKMQLNWKTPAIFAQEKIVETRPQTRNVYLPAGTQWIDFWTGTT
                     YSGGQTIVADAPIDKIPLLIRAGSIVPMGPFLQYSTEKPADPLEVRIYRGADGSFTLY
                     EDENDNYNYEKGAYSTINFYWDDTKNELTIGERNGSFPGMLMERQFQIVLVSPNHGIG
                     IEITPRPDKIIKYLG"
BASE COUNT         1408 a         1038 c         1081 g         1491 t
ORIGIN      
        1 ctgggttatg cgaagcgctt gacggagtgg ttcagtgtgg gcggtcaaat ccgcctggtc
       61 aaacaggatt taggccaaag tgtgataccg aaaatttcta ccattcccga cactaccgag
      121 tatgtagtca aaaacacact gactccgctg gcttttgatt tcgggacgca gttcaaaacc
      181 ggttttaaaa gcttggtctt cgggatgtct attagaaatt tctccaccga cattaaatat
      241 gtcgaagaac cgtttcaact acctttggtt tttaatctgg gaatttcgat ggatttgttc
      301 gatctgatca gtcaaattaa ccagaatcaa tcactgtata tgagtattga tgccagtcat
      361 tatcgttccc gtgctgagca agtgaaaatc ggtctggatt ggcaactatt gaaagtgttg
      421 tcattacggt gcggttatat cactaacgaa gatgagagcg gtttgacttt tggaatcggc
      481 gtatccaaat tcggttttac gtttgactat gcttatacgc cgtatggtgt tttcaatcag
      541 gtacagcgtt tgacagtacg aatgtcattt tgagcgatag aaggagaaaa gtatcatgaa
      601 atctaatcgc tttttaataa tctcagccct ctgtttggca gtaatgaaat gcgaatatga
      661 tgttacccaa cctcaatggt atcaggatta taccgaacca gcaaccccag ctattactaa
      721 tattgagccg gacagtgctg tagccggttg caattatatt aacattattg gtgaaaattt
      781 cagcgggacg ccaacggtct attttgataa tgtaacagcg gaagtaatct ccaatttggg
      841 caattctatc caggttcgtc gtcccaatct ggcaagtgat ggttgtctgg ttaaggtggc
      901 ttgcgatagc gctcttgtgg tcgcgcaaag cactgagcct tataaaatta ctcaggttgt
      961 ccaaaaatat ggtaattttg tcgaaaataa agaattgaat gccgtagcgg ttgatagtgt
     1021 tgaaaatgta tatgtaattg ataacaccaa tattatttac aaagtaaccc cagctggtga
     1081 caaaaccacc atgcccgata aggctaaacg cacaacctat gatgctatca ttggaccaga
     1141 tggaaaattg tatcttttgg gtaacaaccg ctcaattgac gttttagatc cacaaagcgg
     1201 caaggttgct gagtggatta agcttaaggc aggcaaggtg gtaaaatttg gcgattttga
     1261 taagaatggt tatttatata ccggaggtcg gagcacggga ctggttgtcg tcgctccaga
     1321 tcttacgccg aattatacca gtttgtatga aaaagatgaa atcaaaggta ttcgcgtgtt
     1381 tggtaattat gtgtatgtaa gcctggtttg taccaccccc gacttgcaaa atccggctct
     1441 ggcaatttgg cgtaatccaa ttgataatag tggcaatcca ggagaaaaag aattagtgct
     1501 tgatttgacg gcttataaaa actattctgc tgatgcttta aatagctttt cgctttcatc
     1561 tgacggcaaa ttatttctgg gtattaacgc gcagactcct cttcttgcca ttgatcttaa
     1621 caccacgagc gccgattatt tttatctggg tataatagct ccctattgta aatcttttag
     1681 ctgggggtgg ggaaattacc tatatatgat ttgtggcgat acggctcttg gagtagagtg
     1741 gacactctat cgcattgata tgggagtcac cagcgctcgg gaataatctg tctgtcactc
     1801 aactaaagaa aaacggccgg cttttgactg gccgttttgt ttttatgaag aataaaatta
     1861 gagcctgatt gtctgcgctt agcccaaata cttaatgatt ttatccggtc gcggggtaat
     1921 ttcgattccg atgccatgat tgggtgaaac aagcactatc tggaattgac gttccatcaa
     1981 catacctgga aaagaaccgt tacgttctcc aatcgtcaac tcatttttag tatcatccca
     2041 ataaaaatta atagtcgaat aggcaccttt ttcataattg taattatcgt tttcatcctc
     2101 ataaagagta aaactgccat cggcgccccg ataaattcgc acttcgagcg ggtcggctgg
     2161 cttttcggtg gaatattgga ggaaaggtcc catcggtaca attgaaccgg ctctgattaa
     2221 taagggaatt ttgtcaattg gagcatcggc gacaatggtc tgaccgcccg aataggtcgt
     2281 tccagtccag aaatcaatcc actgcgttcc ggcaggtaaa taaacattgc gggtttgggg
     2341 tcgcgtctcg actattttct cttgggcaaa gattgcgggg gttttccaat ttaactgcat
     2401 cttggcggct ccggtcgaac gattttgcat ttcgaggcga aaatcatatc cctgtccagc
     2461 cttcaggttg atcaggtcgg tatattgctc gacactggta taaactatcg gcaactcctt
     2521 gccattgata aaaatgcgtt tagcgtcgta gctttttaag tgaaattgat gcgagccggt
     2581 ctgactgggg atcaatttac cttcccagcg taccgaaaaa gcggaatcgg tcatataatc
     2641 cggtcggccg gtgtaccaga gatgattaat atcgggctca atctgttcgt gagataggat
     2701 tctaaaatct ggatcacgaa aatattgaac ttttaagcct gcttttccat cattagtctt
     2761 aaaatggtca ccgctgatta aaatgctttt tccaggtgga cgatgcaaca tatactccgt
     2821 taccgggcag accataatag ccggaccgaa catatattgg tcgccgagag agtaagtctc
     2881 aggatcgttc gggaaatcca tcggcaaacc gcgcatcata gtgtaatcat cattagtcac
     2941 ccgccaggct aaggaataaa tataaggcat aagccgatag cggagatggt caaattttat
     3001 taggtgcggg ctaaattcac caaagcacca gatttcccgt ggcgtttcac tcccgtgtgc
     3061 ccggaaaatt ggacagaaag ttccaaattg aaacatacga gtataaagtt cttgataagc
     3121 cggatcctga ccgccattaa tgaatactcc accataagcg ccaatgacga atccaccgat
     3181 gtcaaaagtc caataaggaa tgccagccat acaaaaattc agaccggcgc taatttgatt
     3241 ttgataaatc tcccagctgg cgccaatgtc gcctgaccag gttgtggcac cggttctctg
     3301 ttgaccagca aagacggacc gggtcagcat atatactctt ttgcggtcgg aatcctgacg
     3361 ctgattctgg taaagtttat ccatcattac caaagaatat gcgttcagat aacgcgccca
     3421 actgccaagg taattccgac ctaccttttt catttcgtat tcttgggatt ctttggtggt
     3481 ggcattgacg atatccggtt cagtggaatc aatccagagc gcatcgatgc cgaccgagat
     3541 taaaccattt tttatatatt tccagtaaag atcattagcg gctggattga aagcgtcgaa
     3601 atatttgaaa tttgcccatc cgacggtatt gtagaggaac ccgttttgct ccatttcttg
     3661 ataaattgcg gtattcggtc caagtcccgg ccagattgaa atcattatat gaaaattttg
     3721 attgtggatt tgccggatca tttcctcagg ctcgggatat tttttcggat caaagaacat
     3781 ctggctccaa ttttcattgc cgtcccaata attccaatct tggatgatat tatcaatcgg
     3841 tatgcggcgc agacgatatt cactcacaac gcttaataat tcagcgcggt ttttataatg
     3901 ctccttgctt tgccaataac cgtaagccca ttttccatac aaaggagcct ttccagttag
     3961 ataacgatac ccactaatga cggaatccat tgttccgcca taaattaagt aataatcgag
     4021 attgtcaccg acttccgacc aaagtgacat cgtctcagcc gtatcctgaa aaatagtctt
     4081 ggagtaatta tcccacagaa tgccccatcc gcgagtagaa atcaaaaaag gagttaccgc
     4141 gtccgtattg gtttggacga gtttgacagt tcgcccgcgg taattcatat atccgtcttg
     4201 atgttgacca agcccgtaga gcccttcgtc tggtgttaat ttgaaatttt gctgaatgtt
     4261 gaaagctttt tcatacggaa gcgttaccgg agtaaaaacg gcttttttat tttcttttaa
     4321 aatacttgtg ccgctggtcg taaaatattc caaatggcca tctttcttaa ccaggcgaat
     4381 tgtcattcgc tccgatttta ggattacgtc gttgtcattt tcctcaaccg taatagataa
     4441 atccggcaaa ctatctctaa tgaccattaa acttagcttt tcagctgaag attccggccg
     4501 ccacttaatg actcgggcga tgtcgtcagc gtaaaattgg acacgtacat tcaaatcacg
     4561 agtttttaaa tcaaccccat tctgaatggg tttaactttt ccggactgac cgcattgtga
     4621 taccgccagc gctaaaatta ttaacgaata agtaatctgt gatttcattt attcccctca
     4681 ttttttaggt ttgttaaaag accgctggct ataatttttt taataactat cggaactgat
     4741 tcacatttga ccggacttga tggcggctct gcttcaaaaa ccttcgtaat atcaatggaa
     4801 ttatattagt caatcttcac tatgaaaaca agcttttgaa tggtaaaaaa gtagcagatt
     4861 aaaccgatgg agcctaaaat tatataattg gacactgagg cgcacagagg taagccggag
     4921 agcacggagc atttttaagt acttttaagt tctattaagt ttttttaaca tttttaagtg
     4981 cctttaggaa tttaatagta cttaagcaca cttatcag
//
LOCUS       DAOOMU010000119         5007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_20180_length_5007_cov_19.759289, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000119 DAOOMU010000000
VERSION     DAOOMU010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20180_length_5007_cov_19.759289"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /gene="purF"
                     /locus_tag="PLI55_09145"
     CDS             <1..1028
                     /gene="purF"
                     /locus_tag="PLI55_09145"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPB01035.1"
                     /translation="RDLESHGALFQTTSDTEYIIHLFAHSGGDSLVEKFLQVFRRIKG
                     AFSVLILFDDKMIVARDHRGFRPLCIGTLDGSYIFASETCALDLIGATYERDVKPGEM
                     IVLDGTNVKSYQIAPPEPKHCIFEYVYFSRPDSQIFGEYVDKTRRKLGKILAMEKPAP
                     EAEIVISVPDSSNTIALGYSQRSTARFEIALIRNHYIGRTFIHPTQTMRDFTAKIKFN
                     PVGGVLKDRKIVVVDDSIVRGTTLRKLARMLRSAGAKEIHIRIGSPPVKFPCYYGMDF
                     PSREELIANQKSITEIRDYLEVDSLEYISPEGLLNAMSLPAENFCTACFTGHYCEKIN
                     QEKENSC"
     gene            1022..1987
                     /locus_tag="PLI55_09150"
     CDS             1022..1987
                     /locus_tag="PLI55_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HPB01036.1"
                     /translation="MLKVPSKSIAWLDYLFIFRPTLFFAVWIITLAGYSGYFIFSEKQ
                     PAWWSLEVNWKLVLGFIVVTMATGATFILNQLHDIESDKENRKLFLISEEHIKADLAL
                     KIALITIGVSLVLALIINWQFFIALGICVFFWGYLYNYKPFEWKDKPMLGVLVNFISG
                     LCLFICGWIIAGKWQLKDIIYAIPYLLAWTAVAVLTTIPDKEGDLKTGKKTISVHYEN
                     RVTIWLAFICLVLSFALGMYFNDPVISLSCLLSSPLFIIMLFKPTEAWVLRSIRYSIL
                     FLALILCVEFPLFFIVLLINYYLCRFYYLSRFNLNYPTFQVEESV"
     gene            1984..2157
                     /locus_tag="PLI55_09155"
     CDS             1984..2157
                     /locus_tag="PLI55_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPB01037.1"
                     /translation="MIEVNPDCCDLCGTCISVCPKDCITLTESRLIVDDSQCNACQIC
                     VRVCPFKALRLVE"
     gene            2166..>2770
                     /locus_tag="PLI55_09160"
     CDS             2166..>2770
                     /locus_tag="PLI55_09160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB01038.1"
                     /translation="MRKEYDVIVVGGGPAGSTAARFAKTATNTVALFERDREIGIPVR
                     CAEATSIPDLERFVKIDPHWIATTITKARLISPSDQKVWLNLPNDGVILNRRLFDNGL
                     AEIAATQGVEIFTKANVYKIVSDGEQHWEVFVRHLDRDYIVKSKIIIAADGVESRIAR
                     FAGIRTQTVLKDIESCAQVLLGNLDVAENQIDFYFSSRWAPG"
     assembly_gap    2771..2870
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2871..3221
                     /locus_tag="PLI55_09165"
     CDS             <2871..3221
                     /locus_tag="PLI55_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01039.1"
                     /translation="ARQVNPVTGGGILAAITAGKIAGLVAAQAIARNDWSVNVLKEYQ
                     TEWDKNVGKDYARFYKIKEWMTELTDDELDEIATLLQKIPPDDINIMTVFKIALRNKP
                     ALLLEAIKLFAQMI"
     gene            3221..>5007
                     /locus_tag="PLI55_09170"
     CDS             3221..>5007
                     /locus_tag="PLI55_09170"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPB01040.1"
                     /translation="MDDFLTLAGLNEFIALDVETTGLNPQQDAVIEFAAVKFINGKPS
                     NTLNFLCNPGFPISPEIEQLTGIKDEMLKDKLPFEQHLDEVLEFIGRIPLVGHNIGFD
                     LGFLKEYLKNFKSHRKRILYNPLYDTLLLAQAFLFFLYNHRLSTIAEFYGLSAKGAHR
                     AENDALNTGLIFVKLIEEVQSYDLETIQTINMVLKDTDDPNKLLYKNAAEALLLQRSL
                     GSEEAPQIGWNPPVNIFGQSIEKNKESAKASLKIEKFFEPAGPIAQNFVGFEDRPQQK
                     EMAQLVLNSLQAGDSAIIEAGTGVGKSLAYLIPAVVWLNQQKKEKKRVVIASNTKTLQ
                     EQIFYKEIPFVSEKLKLPFKAVLLKGRSNYICLTRWHRYLNALPQQLHLAARSAIIPI
                     IIWLKHTRTGDIAENNGFNLAAHYYIWNEICSEPGYCTTSVCQKYGGCFLGKIRYHAM
                     NADIMVVNHSLLLADSAADNQILPGFDALIIDEAHNLEKNSYNYFAQKVNLPTLIYFL
                     NGIYSGSAPERGLLVDLAESAHQLKSLDKIEPLRQMILEKTSDVKIAAEVFFRKIVAT
                     KFNPANGNQKSFGIKKRYKDFSREFPNLE"
BASE COUNT         1371 a          980 c         1077 g         1479 t
ORIGIN      
        1 gccgagattt ggagagtcat ggggctttgt ttcaaacgac ctcggacacc gaatatataa
       61 tacatttatt tgctcattcg ggcggtgatt cgttggttga gaaattttta caggttttcc
      121 gtcggataaa gggtgctttc tcggttttaa ttttgtttga cgacaaaatg atagtggctc
      181 gtgatcatcg cggattccgg ccgttatgta ttggaactct cgatggttca tacatttttg
      241 cttccgagac ttgtgctttg gatttaatcg gtgctactta tgagcgagat gtcaaaccgg
      301 gcgaaatgat agtattggac ggtactaatg ttaaatctta tcaaatcgct ccacctgagc
      361 cgaagcattg tatttttgag tatgtgtatt tttcacgtcc ggatagccag atatttggcg
      421 aatatgttga taaaaccagg cgcaaattgg gtaaaatttt agctatggaa aagcccgccc
      481 cggaagctga gatcgtgatt tcggtaccag actcgagtaa tactattgcg ctcggttatt
      541 ctcagcgttc aacggctcgg ttcgaaatcg ccttgattcg gaatcattat atcggacgga
      601 cttttatcca tcccacccag actatgcgtg attttacagc taaaattaaa ttcaacccgg
      661 tcgggggagt attaaaagat cgcaaaattg tcgtcgttga tgactcgatt gtgcgcggta
      721 cgactttacg taaattagcg cgaatgttgc ggtccgccgg agctaaagaa attcatatcc
      781 gcattggttc tccgccagtc aaatttcctt gttattatgg tatggatttt ccgtcgcgcg
      841 aagaattaat cgccaatcag aaatccatta ctgaaataag ggattattta gaggttgaca
      901 gccttgaata tataagcccg gaaggtttgt taaatgcgat gagcttaccg gctgaaaatt
      961 tttgtacagc ttgttttacc ggccattact gtgaaaaaat taaccaagag aaggagaact
     1021 catgttaaaa gttccaagta aatcaattgc ctggcttgat tacctgttta ttttccgacc
     1081 gaccttattc tttgcggtct ggattatcac acttgccggt tacagcggct attttatttt
     1141 cagcgagaag caaccagcct ggtggtcatt agaagtcaac tggaaactgg tattaggttt
     1201 tatagttgtt acaatggcga cgggagcgac ttttatctta aatcaactgc acgatatcga
     1261 aagcgataaa gaaaaccgta aacttttttt aatttcagaa gaacatatca aagctgattt
     1321 agccttgaaa attgcactga taaccatcgg tgtttcattg gtattagcct tgataataaa
     1381 ctggcagttc tttattgcgt tgggaatttg tgtatttttc tggggttatt tgtataatta
     1441 taagccattt gaatggaaag acaagccgat gttgggcgtg ctggtcaatt ttatcagcgg
     1501 cttgtgtctt tttatttgcg gctggatcat cgccggtaaa tggcaattga aagacattat
     1561 ttatgcgata ccctatttat tagcatggac ggcggtagcg gttttgacta ctattccgga
     1621 taaagaaggc gatctcaaaa cagggaagaa gaccatctcg gttcattatg aaaatagagt
     1681 tacgatttgg ttggcattca tttgtctggt tttgagcttc gcactcggga tgtattttaa
     1741 tgatccggtc atcagtttat cctgtttgct ttcatcacca ctttttatta ttatgctttt
     1801 taagccgact gaagcctggg tcttgcgatc tatacgctat tcaattttgt tcctggcttt
     1861 gattttatgc gtcgagttcc ccctattttt tattgtacta ttgataaact attatctttg
     1921 ccgattttat tatttatctc gatttaatct gaattatccg acatttcaag ttgaggaatc
     1981 agtatgatag aagttaaccc agactgttgc gatttatgtg ggacgtgtat ttcggtatgt
     2041 ccgaaggatt gtatcacatt gactgaatcg cgcctgatag ttgatgattc ccaatgtaat
     2101 gcttgtcaga tttgtgtgag agtatgccca tttaaagcgc taaggctggt tgaataggaa
     2161 aaattatgcg aaaagaatat gatgtgattg tcgtcggtgg cggccctgcc ggtagtacgg
     2221 cggctcgttt cgctaaaact gctactaata cagtcgctct ttttgaaaga gatcgggaaa
     2281 ttggtattcc ggttcgatgt gccgaagcca cctcgattcc ggatctcgaa cgattcgtaa
     2341 aaattgatcc acactggata gcaactacca ttaccaaagc ccgcttaatt tctccatccg
     2401 atcaaaaggt ctggttgaat ttgcctaatg atggcgtaat ccttaaccgg cgtttattcg
     2461 ataatggatt agccgaaatc gccgctactc aaggagttga aatattcact aaggctaatg
     2521 tttataaaat tgtatcagat ggagagcaac actgggaagt gtttgtgcgc cacctcgacc
     2581 gcgattatat cgtgaaatct aagataatca tagcggctga tggggtggaa tcgcgaattg
     2641 cccgctttgc cggaatccgc actcagaccg ttttaaaaga tattgaatcc tgtgcccagg
     2701 ttttacttgg caatctggat gtagctgaga atcaaataga tttttatttt tccagcaggt
     2761 gggcgccggg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcccggcagg
     2881 tcaatccggt aaccggcggt ggaatcttgg cggcaattac agccggtaaa attgccggtt
     2941 tggtcgccgc tcaagcgatt gcccgcaatg actggagcgt aaatgtattg aaagaatatc
     3001 agacggaatg ggataaaaat gtcggcaaag attatgcgcg cttttataaa ataaaggaat
     3061 ggatgaccga gcttactgac gacgaactcg atgaaattgc caccttactc caaaaaatcc
     3121 ctcccgacga tattaacatc atgaccgtct tcaaaattgc tttacgtaat aaaccggctt
     3181 tattgcttga ggcgatcaaa ttatttgctc aaatgatcta atggacgatt ttttaactct
     3241 cgccggatta aacgaattta tcgccctcga tgttgagacg accggcttga atccgcaaca
     3301 agatgccgtg attgaatttg cggcggtaaa atttataaac ggtaagccgt ccaatacctt
     3361 gaattttctc tgtaatcccg gttttcccat ctctccggaa atcgagcaac tgaccggcat
     3421 caaagatgaa atgctaaaag ataagttgcc ttttgaacaa catcttgatg aagtcctgga
     3481 gttcatcggt agaattccat tggtcggaca taatatcggt tttgatctcg gttttttaaa
     3541 agaatatctc aaaaatttca aaagccaccg gaaaagaatt ctatacaatc cgttatacga
     3601 taccttgctt ttggcgcaag cttttctctt ttttttatat aatcatcgct tgtccactat
     3661 cgctgagttt tatggattaa gtgccaaggg cgcacaccga gcggagaatg atgctctcaa
     3721 caccggcttg atttttgtca aattaatcga agaagtgcag agttacgacc tggagacgat
     3781 ccagactatc aatatggtgc taaaagatac cgacgaccct aataaattac tttataagaa
     3841 tgccgccgaa gctctcttac ttcagagaag cctcggctct gaagaagcac ctcaaatcgg
     3901 ttggaatccc ccagtaaata tttttggtca gtctattgaa aaaaataaag aaagcgcaaa
     3961 ggcttcctta aaaattgaga agttttttga accggctgga ccaattgccc agaacttcgt
     4021 tggttttgaa gaccgaccac aacaaaagga aatggcgcaa ttggtgctta atagtttgca
     4081 ggcaggcgat tcagccatta tcgaagccgg aaccggagtc ggtaaatcac tggcttacct
     4141 aataccggcg gtagtttggt tgaatcaaca gaagaaggag aaaaagcggg tagtaatcgc
     4201 cagtaacacc aagactcttc aggaacaaat attttataag gaaattccct ttgtatcgga
     4261 aaaattgaaa ctgccattca aagccgtttt acttaaggga cgttcaaatt atatctgctt
     4321 gacgcgctgg catcgttatc taaatgctct gcctcaacag cttcacctgg cggcgcgctc
     4381 cgccattatt ccaatcataa tttggcttaa gcatactcgc accggcgaca ttgcagaaaa
     4441 taatggattt aatctggcgg cgcattacta tatctggaac gaaatttgtt ccgagcccgg
     4501 ctattgtact accagtgttt gccagaaata tggcggctgt tttctgggca aaatacgtta
     4561 tcatgcgatg aatgccgaca ttatggtagt taatcattcc ctgcttcttg ccgactcagc
     4621 cgccgataac cagattctac ctggatttga tgctttgatt atagatgaag ctcataatct
     4681 ggaaaagaat tcctataatt attttgcgca aaaagtcaac ctaccaacgc ttatttattt
     4741 tctaaacgga atctatagcg gctcggcacc tgaacgtggc ttgctggtag atttagccga
     4801 aagcgctcat cagctgaaaa gtctcgataa aatcgaaccg ctccgtcaaa tgattctcga
     4861 gaaaacctcc gatgtcaaaa ttgcagcgga ggtatttttc cgaaaaatcg ttgccactaa
     4921 attcaatccg gctaatggca atcaaaagtc ttttggcatt aaaaaacgct ataaagattt
     4981 ttcccgtgaa tttcccaatc tggaaat
//
LOCUS       DAOOMU010000120         4929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_20606_length_4929_cov_27.402544, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000120 DAOOMU010000000
VERSION     DAOOMU010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20606_length_4929_cov_27.402544"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLI55_09175"
     CDS             <1..447
                     /locus_tag="PLI55_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPB01041.1"
                     /translation="LVGNEEDLQKGLGIPGPEITAKSKLDPSAFIAMISDVVKKYPQI
                     KVVATTLREVHSTNRHSWSAVAWINGETYQAPTAELDVYDRVGGGDGFASGFFYGLLK
                     NVPPEEAVKLGWAHGALVTTYPGDTTMATLAEVENFIKGGSARIQR"
     gene            469..1743
                     /locus_tag="PLI55_09180"
     CDS             469..1743
                     /locus_tag="PLI55_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HPB01042.1"
                     /translation="MLYYAKSSADYGLTETEIRDGFFSALAKLGARRKVIAVPPDFTR
                     FHSQAGQLTRLAFQYYGKNLQAILPALGTHFPMTEAEIEAMFGDLPRHLFRIHHWKED
                     LFTLGEVPGEFVFEVSEGKLDYSWPAQVNKLLVEGGFDLILSIGQVVPHEVIGMANYN
                     KNIFVGTGGQEGINKSHFLGAVYGIERIMGRADTPVRRVLNYASDNFARQMPIVYVLT
                     VIGKNSTGQMAMKGLFIGDDYECFQLASELSLTINFDLLDKPLKKVVVYLDPQEFKST
                     WLGNKSIYRTRMAIADGGELIVLAPGLREFGEDPEIDRLIRKYGYRTTPEVMQFVKEN
                     SDLQNNLSAAAHLIHGSPENRFTVIYCPGYISREEIESVNFRYGELAPMLARYDPAKM
                     QDGFNILPDGEEVFYISNPALGLWATKERFNN"
     gene            1770..3221
                     /gene="gnd"
                     /locus_tag="PLI55_09185"
     CDS             1770..3221
                     /gene="gnd"
                     /locus_tag="PLI55_09185"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HPB01043.1"
                     /translation="MNKADIGLIGLAVMGENLVLNMERHGFTVAVFNRTVEKVDKFLA
                     GRGKGKNIIGTHSIPEFIASLKKPRKIMLMVKAGQPVDDFIELLIPHLEKGDIIIDGG
                     NSHFPDTIRRTKYLEEKGFLYIGTGVSGGEEGALNGPSIMPGGSPEAWPHVKPIFQAI
                     AAKVEDGTPCCDWVGENGAGHFVKMVHNGIEYGDMQLICEAYQIMKDLLGLSYPEMHQ
                     VFADWNEGDLKSYLIEITRDILAYKDEDGQPLVEKILDTAGQKGTGKWTVVASLDAGA
                     PLTMIGEAVYARMLSAMKDERVAASKVLKGPQPEFKGDKQQFIADLGQALYAAKIVSY
                     AQGYVLMRMAAKDFGWHLNYGGIALMWRGGCIIRSVFLGKIKEAFDQNPNLSNLLLDP
                     FFKEKIESSQNAWRRVIATAVTNGIWVPALASALNYFDGFRNSRLPANLLQAQRDYFG
                     AHTYERIDKPRGEFFHTNWTGRGGETSSTTYTV"
     gene            3248..4129
                     /locus_tag="PLI55_09190"
     CDS             3248..4129
                     /locus_tag="PLI55_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPB01044.1"
                     /translation="MTLADSLKFEKKHDFLVAIDSDGCAFDTMEIKHKECFIPNIIYY
                     WNLQAVSKYARAAAEFVNLYSKWRGINRFPALTMTFDLLIDWPEVQRRGVTIPRAQSL
                     RDWIARETKLGNPALQAEVERSGDAVLQQALAWSEAVNADIARMVHDVPPFPYVRESL
                     QRLAEVADVMVCSATPTEALRREWEEHNIARYVRAIAGQEVGSKKEQIEQAASARYEP
                     QKILMIGDALGDLKAARANSARFFPINPGHEEDSWENFFKKYLDDFINDQYSEEKEGS
                     LISEFESFLPASPPWKK"
     gene            4156..4929
                     /gene="kduD"
                     /locus_tag="PLI55_09195"
     CDS             4156..4929
                     /gene="kduD"
                     /locus_tag="PLI55_09195"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HPB01045.1"
                     /translation="MENKNVLDLFKLDGKVALVTGAARGLGQAIATALASAGADVSLV
                     DILPTEETHETIAGYGRRALSLTANLMEKANIEPVVDATVKKFGRIDILVNNAGIIRR
                     APLTEFSEKDWYDVINLNQNTVFFLSQMVAKLMIARGEGGKIINIASLLSFQGGILVP
                     SYTASKSAVMGLTRAMANELAPHHINVNAIAPGYMATDNTAPLRADPARAESILERIP
                     IGRWGEPADLQGVAVFLASSASDYMTGYTVAVDGGWLAR"
BASE COUNT         1284 a         1105 c         1302 g         1238 t
ORIGIN      
        1 ctggtcggca acgaagagga tttacagaaa ggtctgggga ttcccggccc ggaaataact
       61 gccaagtcaa agctcgatcc atcggctttt attgctatga ttagcgatgt ggtcaaaaaa
      121 tatccccaga ttaaggttgt ggcaactacc ctgcgggaag ttcattcgac taatcgccac
      181 agctggagcg cggttgcctg gatcaatggc gaaacttacc aagcgccgac ggctgaattg
      241 gatgtttatg atcgggttgg cggcggcgac ggctttgcgt ccggtttttt ctatggttta
      301 ttgaagaatg ttccgcccga agaagccgtc aaactcggct gggcacacgg agctctcgta
      361 acgacttatc cgggcgatac gacgatggca actttagccg aagtagaaaa ttttattaaa
      421 ggcggctcag cccgcatcca gcgctaagcc gttttaggac aatccataat gttgtattac
      481 gctaaaagtt cagctgatta cggactgacg gaaaccgaga ttcgggacgg ctttttttca
      541 gctctggcga aattgggagc gcgccgtaaa gtgatagccg ttccgccgga ttttacccgt
      601 ttccattctc aagctggcca actgacgcgt ttagcatttc aatattatgg caagaatcta
      661 caggcgattt tgccggcgct cggcacacat tttccgatga ccgaagcgga aatcgaggcg
      721 atgttcggtg atttgccgcg ccaccttttc cgcattcatc actggaaaga agacctattt
      781 accctcggcg aggtgcccgg tgaattcgta ttcgaagtct ccgaaggaaa actggattat
      841 agctggccag cgcaggtcaa taaattgttg gtcgaaggtg ggttcgatct gattttatcc
      901 atcgggcaag ttgtgccaca tgaagtgatc ggtatggcaa attataataa gaacattttc
      961 gttggaaccg gcggtcagga aggtataaat aagagccatt ttttaggtgc ggtttacgga
     1021 atagaacgca ttatgggacg cgccgatacg ccggtgcgcc gcgtgctgaa ctatgcttcg
     1081 gataattttg cccggcaaat gccaatcgtc tatgtcttga cggtaattgg aaaaaatagc
     1141 actgggcaaa tggctatgaa aggcttattt atcggcgacg attatgagtg tttccagctg
     1201 gcgagcgaac tgtcgctgac tattaacttc gatctgttgg ataaaccgct caagaaagtc
     1261 gtcgtttatc tcgatccgca ggaattcaag agcacttggc tcggtaataa aagcatttat
     1321 cgaactcgta tggcgattgc cgacggtggt gaattgatcg tgctggcgcc cggtttacgt
     1381 gaatttggtg aggatccgga aattgatcga ctgattcgta aatacggcta ccgcactacg
     1441 ccggaagtta tgcaattcgt aaaggaaaat agtgacttac agaataatct cagtgcggcc
     1501 gcccatttga ttcacggttc gccggaaaac cgctttacgg taatatattg ccccggttat
     1561 attagtcggg aagaaatcga aagcgtcaat ttccgatatg gcgaattggc gccaatgttg
     1621 gcacgttatg atccggcgaa aatgcaggat ggtttcaaca ttctaccgga cggcgaagag
     1681 gtattttata tctcgaatcc ggcgttaggg ctctgggcta ccaaggaaag atttaataat
     1741 tgaattataa tatcaggagg atttaaaaaa tgaataaagc ggacataggt ttaattggat
     1801 tagccgttat gggcgagaat ttggtgctga atatggagcg gcatggtttc acggtggcgg
     1861 ttttcaatcg caccgtcgaa aaagtggaca aatttctggc cgggcgtggc aagggcaaaa
     1921 atatcatcgg cactcactcg attccagaat ttattgcctc gttaaaaaaa ccacgcaaga
     1981 ttatgctgat ggtaaaagcc ggtcaaccgg tggatgattt tatcgaatta ctaatcccgc
     2041 atttagaaaa aggcgatata attattgacg gcggcaattc gcattttccg gatacaattc
     2101 gccgcacaaa atatctggag gagaagggtt ttctgtatat cggcaccggg gtctccggcg
     2161 gagaagaagg cgcgttaaat ggtccgtcaa ttatgccggg tggttcaccg gaggcttggc
     2221 cgcatgtcaa gccgattttt caggcgattg cggctaaagt cgaggacggc acaccgtgtt
     2281 gcgactgggt tggtgaaaac ggcgctggtc attttgtcaa aatggttcac aacggcatcg
     2341 aatacggtga tatgcaattg atttgcgaag cctaccaaat tatgaaagac ctgctcggct
     2401 tgagttaccc cgaaatgcat caggttttcg ccgattggaa tgagggtgat ctaaaaagct
     2461 acctgattga gattacccgc gatattctgg cttataaaga tgaagacgga cagcctctgg
     2521 tcgagaaaat tctggatacc gccggccaaa aaggtactgg caaatggaca gtggtggctt
     2581 cactggatgc cggcgcaccg ctaacgatga ttggtgaagc cgtttatgcc cggatgcttt
     2641 cggcgatgaa agatgagcgc gtcgcggctt ctaaagtttt gaaaggaccg caaccggaat
     2701 tcaaaggtga caagcaacaa ttcatcgccg acctcggtca agccttgtat gcggcaaaaa
     2761 ttgtatccta tgcccagggc tacgttctga tgcgtatggc ggctaaggac ttcggctggc
     2821 atttaaatta cggcggtatc gccttaatgt ggcgcggcgg ctgtatcatt cgttccgtat
     2881 ttctgggaaa aatcaaagag gcgttcgatc aaaatcccaa cctgagcaat ctacttctcg
     2941 acccattttt caaggaaaaa atcgaatcgt cgcaaaacgc ttggcgtcgg gtaattgcta
     3001 ccgccgttac taatggcatc tgggttccag cgctggcttc cgctttgaat tacttcgatg
     3061 gattccgcaa cagtcgcctg ccggctaatc ttctgcaagc tcagcgtgat tatttcggcg
     3121 cacatactta cgagcggatt gacaagccgc gtggagaatt ctttcatact aactggaccg
     3181 gacgtggtgg agagacatct tccacgactt atacggtctg attcatttcc acgaaggaga
     3241 cagaacgatg acgctggcag attcgctaaa atttgagaaa aagcacgact ttctggtcgc
     3301 gatagattcg gacggctgtg ctttcgatac gatggaaatc aaacacaagg aatgctttat
     3361 tcccaatatt atctattatt ggaatttaca agccgtttcg aaatatgccc gcgccgcagc
     3421 tgaattcgtc aatctctatt ccaaatggcg ggggatcaac cgctttccgg ctttgacgat
     3481 gacttttgac ttattaattg attggccgga agtgcaacgg cggggcgtaa ccattcccag
     3541 agctcagtcc ttgcgagatt ggattgcccg cgaaaccaaa ctcggcaatc cggctctcca
     3601 agccgaagtc gaacggagcg gcgatgcggt tttgcaacaa gctctggctt ggagcgaagc
     3661 cgtcaatgcc gatatcgctc gaatggttca cgatgtaccg ccgtttcctt atgtaagaga
     3721 atcattacag cgtcttgccg aagtagctga tgtaatggtt tgctcagcga cacccacgga
     3781 agctctgcgg cgcgaatggg aagaacataa cattgcgcgc tatgttcggg cgattgccgg
     3841 gcaggaagtt ggcagtaaaa aggagcagat cgagcaggcg gcaagcgcaa gatatgaacc
     3901 ccagaaaatt ctaatgattg gcgacgctct cggcgatctc aaagccgccc gcgctaattc
     3961 agctcgtttt ttcccaataa atccgggaca cgaggaagat tcctgggaga attttttcaa
     4021 aaaatacctg gatgatttca tcaacgacca atattcagaa gaaaaggaag gttctctgat
     4081 tagtgaattt gagtcgtttc taccggcgtc tccgccgtgg aagaaataaa tttcaagaca
     4141 attaaagagg agtatatgga aaacaaaaat gttttagatt tattcaaact ggacggtaaa
     4201 gtcgcgctgg ttaccggtgc cgcgcgggga cttgggcagg cgattgccac ggctctggct
     4261 tcagctggag ccgacgtcag cttggtagac attttaccaa ctgaagaaac tcacgaaact
     4321 attgctggct atggtcgccg agcgctcagc cttaccgcta atttgatgga aaaagccaat
     4381 attgagccgg tagttgacgc cacagtgaaa aaattcggcc gcattgatat tctggtcaat
     4441 aatgccggaa ttattcgacg cgcaccgctg acggaatttt ccgaaaagga ctggtacgat
     4501 gtcattaacc ttaatcagaa tacggtcttt ttcctcagtc agatggtggc taaattgatg
     4561 attgcgcgag gagaaggcgg taagattatc aatatcgctt cactattgtc ctttcagggc
     4621 ggaattctgg tgccgtccta taccgcttcg aaaagcgccg ttatgggatt gacccgcgca
     4681 atggctaatg aactcgcgcc gcatcatatc aatgtcaatg ccatcgcacc cggttatatg
     4741 gcgaccgata acaccgcgcc cctgcgcgct gatccggcga gagcagaatc cattcttgag
     4801 cgtattccta tcgggcgctg gggcgaaccg gctgacttac aaggcgtggc ggttttccta
     4861 gcttcgtcag catccgatta tatgaccggc tataccgtgg cagttgacgg cggctggctg
     4921 gcaagataa
//
LOCUS       DAOOMU010000121         4743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_21697_length_4743_cov_3.977602, whole genome shotgun sequence.
ACCESSION   DAOOMU010000121 DAOOMU010000000
VERSION     DAOOMU010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21697_length_4743_cov_3.977602"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="PLI55_09200"
     CDS             complement(<1..1703)
                     /locus_tag="PLI55_09200"
                     /inference="COORDINATES: protein motif:HMM:NF019113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HPB01046.1"
                     /translation="MKKHLYLQLLILIIPQLLAAGNNDLLIQEIGQQLIRKDEVFNGR
                     FPEYTENGIWQFRKNPNWLSGFIGGELWYLYEMTGEEEYKLRALKHADQMIDYATIDY
                     THDMGFIFMPTCVKSYQMTKDAKYRDAAIKAADMLVKRFNDKGQYIRAWGKLNSNDEA
                     GLMIIDTIMNLELLFWAAQETGNYKYYEIAYRHALTAMKELVRPDGSSFHVVEFDPES
                     GQVLKKFTHQGFADSSTWSRGQAWGIYGFTLAYEYTQDERFLKTAEIMADYFIKHLPK
                     DQVPYWDLSLSEEGTLRDASAGAIAASGFFELADVASKKQEEQKYRRIASDITSSLVN
                     NYLFSQTSRRDEEGILLHTIYHFHKKWGVDESFPAGDYYLIEALKRQWDLEQFQRLDE
                     SNSARQIINLNKNWYYLEEAIPYINHLHLVAQEWEKINLPHTWNVTDATDNIPGYRRS
                     TSWYQKEIFIPTLNYDQCLLLSFESVNISCIVLVNGQKVGEHVGGYLGFDVDITPFIK
                     PNEKNIILVKVDNSYNPDIIPSQKSDFFIYGGITRDVWLKVMPAEYIEKILITIPQVS
                     K"
     gene            complement(1705..2880)
                     /locus_tag="PLI55_09205"
     CDS             complement(1705..2880)
                     /locus_tag="PLI55_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019388497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HPB01047.1"
                     /translation="MNFNNGKIALILLLASEVFFLCSGKAVNINIAEVDHDRILTAAD
                     RYLNEKPITITSYPCERSAGGIHDFYSEGDYWWPNPDDPNGAYIRRDGISNPDNFIKH
                     RQLMSRLSIQVPTLVAAYKLTHDKIYADKAVKHLQAWFVADETRMNPNLMYAQAIKGF
                     VTGRGIGIIDTIHLVEVAQSITILENNQAIGSADLQAIKKWFSEYLDWIFTHEYGIAE
                     RDNGNNHSTCWALQVAEFAKLVDDQDKVTFCRNFYKTVLLPEQMAIDGSFPKELARTK
                     PYCYSLFNLDAMAMICHILSTEEDNLWYFQLKDGRGMQRAVEFMFPYVRNKSNWPFPP
                     DVMYFDLWPVRQPFLLFAGIAYNESEYIDLWKTLNPDPDNEEIVRNYPIRQPILWMN"
     gene            complement(2889..>4743)
                     /locus_tag="PLI55_09210"
     CDS             complement(2889..>4743)
                     /locus_tag="PLI55_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB01048.1"
                     /translation="AQGVWQSQILYYGSDSNLVYIPDSEGNRIPDFSYAGYKGGLEPI
                     PDIPVVKTLSPISGDNTTRIMNALSQVYNMPIDENGFHGALLLEPGVWEITGKIVFKY
                     SGVVLRGCGAGEDETTNTILRATGNSPAKRTVLVVGGGSTSCWRDEVSGTRTNIITDT
                     IYVGEREFEVEDVSSYSVGDNIIIYHPCTEEWLEAIDYGGTHSGEPGAEADDVPWTVN
                     SQPLVFNRYITAIQNNRITIDAPVFNTLIKSLSQSYIYKYRRDNLKTNIGIENLRIDI
                     ETAGGTDEEHAANAIDLYQIEDAWVRNCTMLHFSASGVRTNTASRITIENCKALDPIS
                     VIEGEKRYNFQVYTASQQILFKNCHATNGRHHYISNGTSWTSGIVFLNCTSSGAYTSS
                     EGHRRWSMGLLFDNHIELDGPRAGVNPRLLGLYNRGYYGTSHGWSSAHSVAWNCNVAK
                     GDLVVQKPPTAQNYAIGCFGGKVTGTNSPSLFDEPEGYIEGANVAGLEPQSLYLAQLE
                     ERRRKVNVSVSGNLNSSNRFKLLPNFPNPFNASTTIPFSLKVAADVKLVIYDVQGKVV
                     KILIDKHLPAGDHLPVWNGRDNSGKISPTGLYFYVLNTGKQVCSGKMVLIK"
BASE COUNT         1432 a         1043 c          845 g         1423 t
ORIGIN      
        1 tttttcgaaa cttggggaat agtgattaat attttctcga tatattccgc tggcattacc
       61 ttaagccaaa catcacgagt aataccacca taaatgaaaa aatccgactt ttgcgatgga
      121 ataatatcgg gattatatga attatcaacc tttaccaata ttatattttt ttcatttggt
      181 ttgataaagg gcgtaatatc cacatcaaat cctaaataac caccaacatg ttcgccaacc
      241 ttttgcccat ttaccaagac tatacagctg atattgacac tttcgaaact tagcaacaaa
      301 cattgatcgt agttcagggt aggaataaat atttctttct gataccagct ggtactcctc
      361 cgatagcctg gaatattatc ggtcgcatct gtaacattcc aggtatgcgg tagatttatt
      421 ttttcccatt cctgagccac taaatgtaaa tgatttatat aaggaattgc ctcttccaga
      481 taataccagt ttttatttag attaataatt tgccgtgcag aattgctctc atctagtcgc
      541 tgaaattgct ccaaatccca ttgtcttttt aaggcttcga ttaaataata atcaccagcc
      601 ggaaaggatt catctactcc ccacttctta tgaaaatggt aaatagtatg aagcaagatt
      661 ccctcttcat cacgtcggga agtttgacta aaaaggtaat tattaactaa actgctagta
      721 atatcgctag caattcgacg atatttctgt tcttcttgtt ttttactagc tacatcagct
      781 aattcgaaaa atcccgaagc cgcaatggca ccggcagagg catcacgtaa agtaccttcc
      841 tcagaaaggc tgaggtccca atatggtacc tgatcttttg gtaaatgttt aataaaatag
      901 tcagccatta tttcggcggt cttcaaaaaa cgttcatcct gggtatattc ataggccagc
      961 gtaaaaccgt aaattcccca agcttgaccg cgggaccaag ttgagctatc tgcaaaaccc
     1021 tgatgagtaa acttcttcaa gacttggcca ctttctgggt caaattctac tacgtgaaaa
     1081 gaagaaccgt caggtctaac cagttctttc atagctgtca aagcatggcg ataggcgatc
     1141 tcataatatt tatagttacc ggtttcctga gccgcccaaa acaacaattc caaattcata
     1201 atcgtgtcaa taatcatcaa accagcctcg tcattggaat tcagtttacc ccatgcccgg
     1261 atgtattgcc ccttatcatt gaatcgtttg actagcatat cagcggcctt aatcgctgcg
     1321 tcccgatatt ttgcatcctt ggtcatttga taagatttaa cacacgttgg catgaaaata
     1381 aaacccatat cgtgagtata atctatagtg gcataatcaa tcatttgatc agcatgtttt
     1441 aaggctctta gcttatactc ttcttcgccc gtcatttcat aaaggtacca taattcccca
     1501 ccaataaagc cggatagcca attgggattt ttacgaaatt gccatatccc attttccgta
     1561 tattctggaa agcgaccatt aaaaacctca tctttacgga tcagctgttg gccaatttct
     1621 tgaattagca gatcattatt gcctgccgcc agcaattgcg gaataattaa aataagtaat
     1681 tgcaagtaaa gatgtttctt caatttagtt catccataat ataggttgac gaattgggta
     1741 atttcttact atttcctcat tgtcaggatc aggattcaaa gttttccata ggtcaatata
     1801 ttccgattcg ttatatgcga ttcccgcaaa tagtaaaaat ggttgccgta ctggccataa
     1861 atcgaaatac attacatcgg gtggaaaagg ccaattagac ttgtttcgaa cataaggaaa
     1921 cataaattcc actgctctct gcataccacg gccatccttt agttgaaaat accaaagatt
     1981 atcttcctca gtcgataata tgtgacaaat cattgccata gcatctagat taaacaggga
     2041 gtagcaatat ggtttcgtac gggcaagttc tttcgggaaa ctaccatcaa tcgccatttg
     2101 ctcaggtaat aaaaccgtct tgtaaaaatt gcggcaaaaa gtaaccttat cttggtcgtc
     2161 aactagcttt gcaaattctg cgacttgtag cgcccaacag gtactgtgat tgttaccatt
     2221 atcccgttca gcaataccat attcatgggt aaatatccaa tcgagatatt cgctaaacca
     2281 cttcttaata gcttgtaaat cagcagatcc aatagcttga ttattttcca atatagtaat
     2341 tgactgggcg acttcgacca aatggattgt atcaatgatg ccaataccac gcccggttac
     2401 gaatcccttg atagcttgag cgtacattag attgggattc atacgagtct catcggcaac
     2461 aaaccaagct tgcaagtgct ttacggcttt atcagcataa attttatcat gagtaagttt
     2521 ataggccgca actaatgttg gaacttgaat actaaggcgg gacatcaatt ggcgatgctt
     2581 aataaaattg tccggattac ttatcccatc tcgacgaata tacgctccgt ttgggtcatc
     2641 tggatttggc caccaataat cgccttctga ataaaaatcg tgaatcccgc cagccgagcg
     2701 ttcacaggga taagacgtta tcgtaatagg tttttcatta agataacgat cggccgctgt
     2761 caaaatacga tcatgatcaa cttcggcaat attaatgtta accgcttttc ccgaacatag
     2821 aaaaaaaacc tcactagcta gtaataaaat aagtgcaatt tttccgttat taaaattcaa
     2881 attgattttt atttaattaa aaccattttc ccggaacaga cttgttttcc ggtatttagc
     2941 acataaaagt ataaacctgt tggtgaaatc ttaccgctat tatcacgtcc attccatacc
     3001 ggaagatgat ctccggcagg taagtgttta tcaattaata ttttaacaac cttaccctgg
     3061 acatcatata tgactaactt aacatcagcc gcgaccttga gtgagaaagg gattgtagta
     3121 ctggcattaa atggattggg aaaattgggt aataatttaa atctattgct ggaattcaga
     3181 tttcctgata cacttacatt tacttttcga cgccgctctt ccaattgtgc caaatacaga
     3241 gattgtggtt caagtccggc aacatttgca ccttcaatat aaccttccgg ttcatcaaat
     3301 agtgaaggag aattcgttcc agtaaccttg cctccaaaac aaccaatcgc gtaattctga
     3361 gccgttggcg gcttttgcac gacgaggtca cccttcgcta cattgcagtt ccaagctacg
     3421 gaatgagcac ttgaccaacc gtgactggtt ccgtaatatc cccgattata aagaccaagc
     3481 aagcgcggat ttaccccggc tcgggggcca tccagttcta tatgattatc aaataatagc
     3541 cccatactcc agcggcgatg gccctcacta ctcgtatagg cgccggacga ggtgcaattg
     3601 agaaaaacaa ttcctgatgt ccaggatgtt ccattggata tatagtgatg ccttccgttt
     3661 gttgcatgac aatttttaaa taatatctgc tgagacgcag tataaacttg gaaattgtag
     3721 cgcttttcac cttctataac cgatattgga tccagtgctt tacaattttc gattgtaatt
     3781 cgggaagcag tgttagtccg aactccagat gccgaaaaat ggagcatagt gcaattccgc
     3841 acccaggcat cttcaatttg ataaagatca atcgcattgg cggcatgttc ttcatctgta
     3901 ccgccagcag tttcaatatc gatccgcaga ttttcgatac cgatattggt ttttaaatta
     3961 tcccgtctat atttataaat atacgactgg gaaagactct taattaaggt attgaatacc
     4021 ggggcatcta tggtaatccg gttattttga atagccgtga tataccggtt gaaaactaat
     4081 ggttgactat ttactgtcca agggacatca tccgcttctg ctcccggttc cccggaatga
     4141 gttccaccgt agtcaatggc ttctaaccat tcctcagtac aaggatgata aataatgata
     4201 ttatctccaa cagaatacga ggaaacatct tcaacttcaa attcccgctc tccaacataa
     4261 attgtatcag taataatatt agtccgagta ccgctaactt catcccgcca gcaagacgta
     4321 cttccgccac ccacaactaa aactgtccgt ttcgcaggag aatttccggt ggcacgtaaa
     4381 attgtattcg tagtttcgtc ttcaccagca ccacaaccgc gcaagactac tccagaatat
     4441 ttaaatacaa tcttcccggt aatttcccaa acccccggtt ctaataataa agcaccgtga
     4501 aaaccatttt catctatggg catgttataa acttgcgata atgcattcat tatacgggta
     4561 gtattatcac cacttattgg cgaaagggtt tttacgacag gaatatcagg aatgggctcc
     4621 agcccacctt tataaccggc ataactaaaa tccggaatac gattaccttc tgaatcaggg
     4681 atatatacaa gattactgtc gcttccataa tataggattt gagattgcca aactccctga
     4741 gca
//
LOCUS       DAOOMU010000122         4581 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_22702_length_4581_cov_20.618869, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000122 DAOOMU010000000
VERSION     DAOOMU010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22702_length_4581_cov_20.618869"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..270)
                     /locus_tag="PLI55_09215"
     CDS             complement(1..270)
                     /locus_tag="PLI55_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01049.1"
                     /translation="MPKKLSNKRLLELRTECEHLLERLGYIVVYGLGNFKEGACLVQS
                     EKKIVINQYTPLDLQVDFLLKILKSQDLSGVFILPALRNLLEQDK"
     gene            complement(276..1343)
                     /locus_tag="PLI55_09220"
     CDS             complement(276..1343)
                     /locus_tag="PLI55_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPB01050.1"
                     /translation="MYSVIMAGGIGKRFWPRSRRERPKQLLDVIGETTMLQMTYERLK
                     SVSDEKKIFIITAQNMKESILKTLKGFPEKNIICEPYGKNTAPAIGLATALIVNKDPE
                     AVIGVFPADHYISNIDEFKKSIGIGFRFARDHTALVTFGIKPTRPATGYGYIQVDKNV
                     KVIEDSVYKVKTFAEKPNLSTAERFLESGEFLWNSGMFIWKGINILNAIKNFIPELYA
                     CLQSIAKSIDTPEYESTLKRHWAMIQSISIDYGILEKAKNVYVVQGNFDWSDVGSWDA
                     VYDLKTKDKNGNVLIGNVATLDTHGCYFYSRKNLLAAVGVSDLIIVQSKDSILVVNRG
                     DSERVKDLVEMLAREGFADHL"
     gene            complement(1494..3197)
                     /locus_tag="PLI55_09225"
     CDS             complement(1494..3197)
                     /locus_tag="PLI55_09225"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPB01051.1"
                     /translation="MSVLKLMITFTRQIKWASLIAICAVLIGCAAQGGPGGGPVDRTG
                     PVLLASFPQDGATNVDLNSDLILNFSEPIDARITDGILQVTPNLSTAPQVKVKHRKVI
                     VKLNEPLQPDRTYIFNFGRNLKDYQNNATAQEIKIAFATGDSIDQGMIAGVVTDIPTN
                     TKTEVWFYKKNTVFPDSLWLAEPDYIISVDKDGNYQATNLPVGEYRALAVSGERLRPK
                     FVSENDLLALPQTEPLIIKNRHDYLENINFRLSKRYLKPFRLINAKPIDGYLELNFSR
                     PLMDTRLMPANFVFADEKVIVKHAWISEEQPTRIILLAENLTPQSEHQISVSGIRDVE
                     ADTLPIDSRTAQFIWTTRSDTLKPKIISTNPPANATNVELSTAIQINLSEPVYQDTLA
                     QNIALFYHDTVNVAIHSSWIDANSFIIEPQKPLISASNYLLQINSRNWCDYAGNFFQD
                     SLFILKFSTIDQNLFGSISGKIISRDGGNLKNLIIDAQLTGSDKFFRQTRPDSTGFYE
                     FIDLLPGKYKFAIWEDRNCNGKYDFGRLAPYLPAEPYRTYQDEINVRSRWETAEVNWS
                     Y"
     gene            complement(3266..4447)
                     /locus_tag="PLI55_09230"
     CDS             complement(3266..4447)
                     /locus_tag="PLI55_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPB01052.1"
                     /translation="MDLFKKCYAYKDAQIARENGYYPYFIPFSGGDDTVMIYKGQPKI
                     MIGSNNYLGLTHHPKVIEAAQKALNEYGTGRTGSRFLNGTLDIHEEFERELAEFTNKE
                     AALVFSTGYLVNMGVISSLLHKKDYAIIDRLNHASIVDGCRFSGANTVRYRHNDMSDL
                     AKVLKNVPDNSGKLVIVDGIFSMEGDIANLPEIVQIAKASGARIMVDDAHSLGVLGPN
                     GNGTAAHFGLSDQVDLIMGTFSKSFGAVGGFVSGEFEVIDYIKHIARSMIFTASLPPS
                     VVASTRAALQIIKSEPERRQRLIDIGNYMRREFKRLGFNTSTSETPVIPIVIGDDMKT
                     FTFWKMLFEAGVYTNAVVSPAVPSNSSRLRTSYIATHTQEQLDFVLDKFAQIGKQLAI
                     I"
BASE COUNT         1313 a          970 c          971 g         1327 t
ORIGIN      
        1 ctacttatcc tgctcgagca aattgcgcaa agccggtaaa atgaaaacgc cggataaatc
       61 ctgacttttt agtattttta gtaaaaagtc aacctgcaaa tccaacggag tgtattggtt
      121 gattacaatt ttcttttcac tctgcaccag acaagcgcct tccttaaagt ttcccagtcc
      181 gtaaacaacg atataaccga ggcgttcgag taaatgttcg cattcagttc gtaattccag
      241 gagacgttta ttggataatt tcttaggcat attatttaca ggtgatcagc aaagccttcc
      301 cgcgccagca tttcgactaa gtctttgact ctctctgagt caccgcgatt tacgaccaga
      361 atggaatctt tagattgcac gatgattaaa tcgctcactc cgacggccgc taacaaattt
      421 ttccgcgaat agaaataaca accatgggta tccagagtag ctacgttgcc aatcaaaaca
      481 tttccgttct tgtctttggt ctttaagtca tatacagcgt cccatgaacc gacatcgctc
      541 cagtcgaaat ttccctgaac aacgtagacg ttcttggctt tttccagaat tccataatca
      601 attgaaattg actgaatcat cgcccaatgg cgtttcagag tggattcata ttcgggcgtg
      661 tcgatagatt tagcgatgga ttgtaagcag gcgtataatt cggggatgaa gttttttatg
      721 gcatttaaaa tattgatgcc tttccagatg aacataccgc tgttccagag aaattcgccg
      781 ctttccagaa atcgttcggc tgtagataga ttgggttttt cggcaaaggt tttcacctta
      841 taaactgaat cttcgataac tttgacgttt ttatcaactt ggatataacc gtagccggtt
      901 gccggacgag tcggtttaat tccaaaggtt acaagagcgg tatgatccct ggcgaatcga
      961 aaaccgatac cgattgattt tttaaactcg tcgatattgc tgatataatg atcagctgga
     1021 aaaacgccaa taactgcttc cgggtcttta ttaacgatta aagctgtagc cagaccgata
     1081 gctggcgccg tatttttacc ataaggttca catattatgt ttttctccgg aaagcctttt
     1141 aaggttttta aaatactctc tttcatattt tgggcagtaa tgataaagat tttcttctca
     1201 tctgaaacac ttttcaatcg ttcataagtc atctgaagca tagtggtttc accaatgaca
     1261 tctaataatt gcttcggacg ttcgcggcga ctgcgaggcc agaaacgctt accaattcca
     1321 cctgccataa ttacggaata catttttgaa tactcctatt taaactaaaa aatttcacaa
     1381 gagactgcgc aaggaaatcc actctgaatt tttataaaat aaaggtgagt ctgctaaaga
     1441 atataacagg atgttatcag cctttaatct tttctgataa ttcaccgcag tcattagtaa
     1501 ctccaattaa cttcggctgt ttcccagcgg gaacgtacat taatttcgtc ttgatatgtt
     1561 cgatacggtt ccgccgggag atagggtgct aagcgcccga agtcgtattt tccattgcaa
     1621 ttgcggtctt cccagatagc aaatttatat tttccaggta gtaaatctat gaattcgtaa
     1681 aatccggtac tgtcaggccg agtctggcgg aaaaatttat cagagccggt gagttgagca
     1741 tcaataatca gatttttgag attaccgcca tctctggaaa taattttacc cgaaatactg
     1801 ccgaacaaat tttgatctat tgtactgaat ttcaagatga ataaagaatc ctgaaaaaaa
     1861 ttaccggcat agtcgcacca atttcgggaa ttgatctgta acaaataatt ggaagccgaa
     1921 attagtggct tctgcggttc aatgataaaa gaattggcgt caatccagga tgaatggatt
     1981 gcaacgttta ctgtatcgtg gtaaaaaagg gcaatgttct gcgctaaagt atcttgataa
     2041 accggttcac tcaggttgat ttggattgcc gttgacaatt caacgttggt ggcgttagct
     2101 ggtgggtttg tcgagataat tttcggcttt agggtatccg agcgagtcgt ccaaataaat
     2161 tgggcagtgc ggctgtcaat gggaagagta tcggcttcta catctctgat tcccgacacg
     2221 ctaatctggt gttcgctttg aggcgtaaga ttttcagcta acaaaataat ccgagttggt
     2281 tgctcctcac ttatccaggc gtgttttact ataacctttt catccgcaaa aacaaaattg
     2341 gctggcatca atcgagtatc catcaaaggg cgagagaagt tcaattccaa ataaccatca
     2401 atcggtttag cgtttattag ccggaacggt ttcaaatacc tttttgataa gcgaaaattg
     2461 atattttcaa gataatcgtg ccggttttta ataataagcg gctcagtttg gggcagtgcc
     2521 agcagatcgt tttcggagac gaatttagga cggagtcttt caccggaaac ggccagagcg
     2581 cgatattcac cgaccggtaa attagtcgcc tgataattgc catctttatc caccgaaata
     2641 atataatccg gctcagcaag ccagagggaa tcggggaaaa ccgtattttt tttatagaac
     2701 caaacctcgg ttttggtatt agttggaatg tcagtcacga ctccagctat cattccttgg
     2761 tcaatcgagt cgccggtggc aaaggcaatt ttgatttcct gagcggtggc attgttctgg
     2821 taatctttca gattgcgccc aaaattgaag atgtaggtgc gatctggttg taatggctcg
     2881 tttagtttga cgatcacctt acggtgtttg acttttacct ggggcgcagt tgagaggttc
     2941 ggtgttactt gcaaaatccc atcagttata cgtgcatcaa ttggctcaga aaaattcaag
     3001 atgagatcag agttaagatc aacgttggta gcgccatctt gtggaaaact tgccaaaagc
     3061 accggaccgg tgcgatcgac tggtccccca ccaggaccgc cctgagccgc acacccgata
     3121 agaaccgcac aaatcgctat cagagatgcc cattttatct ggcgtgtaaa ggttatcatt
     3181 aatttgagaa ctgacattat actaagttta ttagaacaga aatgtaggga atcgttccgg
     3241 ttcggtttaa ccggtttaat tgtcactaaa taatggctaa ttgtttacct atttgggcaa
     3301 atttgtccaa aacaaaatcc agctgttcct gggtatgagt ggcgatatag ctggtccgaa
     3361 ggcgcgaaga gttgctggga actgccggac tgacaacggc attggtataa acaccggctt
     3421 cgaataacat tttccagaac gtgaaagttt tcatatcgtc accaataaca atcggaatca
     3481 ccggggtctc gctagtcgag gtattaaatc ccaaacgctt aaattcccgg cgcatataat
     3541 taccaatatc aattaatcgc tgacggcgtt cgggttcaga tttgatgatc tggagagcgg
     3601 cccgggtaga tgccaccaca ctcggcggca gactggcggt aaaaatcata ctccgggcga
     3661 tgtgtttgat gtaatctata acttcaaact cgccggacac aaagccgccg acagctccga
     3721 aagacttaga gaaagtgccc ataatcaaat caacctgatc actcaaacca aagtgagccg
     3781 cagtgccatt accattaggt cccagaacac ccaaagagtg ggcgtcatca accataattc
     3841 tagccccgga agctttagca atctgcacaa tttcgggcaa gttggcgata tcgccttcca
     3901 ttgaaaagat gccgtccaca attactaatt ttccgctatt gtctggcacg tttttcaaga
     3961 ctttagccag atcgctcata tcgttatggc gataacggac ggtattagcc ccggagaaac
     4021 ggcaaccatc cacgatgctc gcgtgattaa gccgatcaat aatagcataa tcttttttat
     4081 gtaggagact tgaaataact cccatattaa ccagataacc ggttgaaaat accagagcgg
     4141 cttctttgtt ggtgaactca gccagttccc gctcgaattc ctcatgaatg tccagagttc
     4201 cgttcaaaaa ccgactaccg gtgcggccgg tgccatattc gttgagtgcc ttctgagcgg
     4261 cttcaataac ttttggatga tgagtcagtc ctaagtaatt attagaacca atcattattt
     4321 tgggctgacc tttataaatc atcaccgtgt cgtcaccacc cgaaaatggt ataaaatagg
     4381 gataatatcc attttcacgc gcaatctgag catctttata agcgtagcac tttttaaata
     4441 aatccattgt tcacaccgta ataaatttca atatgcgttg caaatttaaa gtgggatata
     4501 ttgatttgca aattgataaa taactgaggg aagcataaca aatagtgtgg cgctcacctg
     4561 gtataattta aagcttgtga t
//
LOCUS       DAOOMU010000123         4552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_22910_length_4552_cov_22.802090, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000123 DAOOMU010000000
VERSION     DAOOMU010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22910_length_4552_cov_22.802090"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLI55_09235"
     CDS             complement(<1..480)
                     /locus_tag="PLI55_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HPB01053.1"
                     /translation="MEWTELIVLVISFVFLLLAGVPISFSIGIATLSTMLLSIDTLPA
                     LTTVAQRMATGIDSFALLAIPFFILSGLLMSRGGIARRLIDFAKVLVGRWPGGLAYVN
                     ILAAMFFGAISGSAAAAAAAIGGFMTPLMEKEGYDTNYSASVNITSATTGLLIPPSNI
                     "
     gene            complement(467..1000)
                     /locus_tag="PLI55_09240"
     CDS             complement(467..1000)
                     /locus_tag="PLI55_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPB01054.1"
                     /translation="MLKIRKILDNILKWVVVVLMAISVFNVLWQVFTRFILRNPSSYT
                     EELARYLLIWVGLLGAAYAAGNKMHLAIDLFSAKMKRNNALRLQIFIQICVLFFAISV
                     MVIGGLRLVTITLTLNQLSAALKVKLGYVYMVLPISGILIAIYSIVEIIGLFGQLFRP
                     DSTNNLPEKRTEYHGVD"
     gene            complement(1000..1995)
                     /locus_tag="PLI55_09245"
     CDS             complement(1000..1995)
                     /locus_tag="PLI55_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPB01055.1"
                     /translation="MRTKFLTIIGLGTAIIISGFCLNGCESEARTKTIKLAHALDTSH
                     PVHKSLVYMSERFAEKSGGKYRIDIYPSGQLGSEREYVELLQIGSLGMTKVSCSVLEG
                     FVDKMKVFSMPYLFRDDEHRFKVLEGEIGRELLLEGEKYWIRGLCYLDAGCRSFYSVK
                     KPINTPEDLRGLKIRTQESTTSIQMIQAMGGSATPIAWGELYTALQQGIVDAAENNPP
                     SFYLSHHHEVAKYFALDEHTAVPDILLISTKVWNDLPPQVQEWLQSAADEAAIYQREL
                     WKIATQEALEEVQKAGVVVSYPDKRPFQEKVKDIYESYRSDPEIYELIQRIRAVQ"
     gene            complement(2013..3416)
                     /gene="uxaC"
                     /locus_tag="PLI55_09250"
     CDS             complement(2013..3416)
                     /gene="uxaC"
                     /locus_tag="PLI55_09250"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HPB01056.1"
                     /translation="MILPSDRFFDSDPAIRSLARELYQQVKDLPIISPHGHIEAKILA
                     ENQPFPDPTALFILPDHYIYRLLYSQGIPLESLGISTRDGTPVETDHRKIWQIFADNY
                     YLFAGTPTSLWLAYEFSEVFGVTEKLNSANAMAIYDHLQNQLQKPEFRPRALYERFNI
                     EVLTSTDGATDSLKYHRQIKESGWKGRIVPCFRPDALFNINAVGWRQEIEKLGDAVGY
                     EVNTYSKFITAIENRREFFKTMGAVSTDQGVESAYTHRLSASQAEEIFQRARNGKASA
                     EDARLFTANMLMEMARMSVADGLVMQMHVGALRNHNLRNFRRFGPDTGADIPVRSEFT
                     VNLRELLNEYGTDSRLTLVVFTLDESTYSRELAPLAGYYPAMKLGAAWWFHDSIQGMI
                     RFREMVTETAGFYNTVGFTDDTRSFPSIPARHDLCRRVDSNFLAGLVVRHIIDLDEAH
                     VLIRALTYDLAKKTYKL"
     gene            complement(3413..4468)
                     /locus_tag="PLI55_09255"
     CDS             complement(3413..4468)
                     /locus_tag="PLI55_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPB01057.1"
                     /translation="MNKPPNRSPNVTLDDIARQLKVSKVTVSKALRDHPDIGSATKRE
                     VQALAAKLGYVPNFMARNLSARSSRTIGLVVPKLAHYFFATCIESIYNAANASQYDII
                     MTVSQENPEYELKHIQTLLAMRVEGLLVSVTENTTDTSIFQDIKKRGVPIVFFDRIPF
                     GLESNYVVTDDRAAAARLTNYVIQAGYRKIAHFAGYSHTNIGYERQTGFRQALSDNNL
                     PIREDWIIEGGFGERAGYEALKKVAETKDLPELIFAVTFPVALGIFEAASELGLKIPG
                     DIDLVCFGGGQFNRYLSPALTFMDQPASAIGEKAFNLLLDELHSSERRSEQHIVVPSQ
                     LVIGETCRKLQYNYEEL"
BASE COUNT         1291 a         1103 c          960 g         1198 t
ORIGIN      
        1 gatattactg ggaggaatca ataatccagt cgtagctgag gttatattaa ccgatgcgga
       61 ataattggtg tcgtagccct ctttttccat cagtggggtc ataaaaccgc caattgccgc
      121 ggccgccgcc gccgctgaac cggaaatcgc cccgaaaaac atcgccgcca ggatattgac
      181 ataagccagc ccacccggcc agcgtcccac caacaccttg gcaaaatcaa tcaggcgccg
      241 cgcaatccca ccccgactca ttaataagcc agataagata aagaacggga ttgccagcaa
      301 ggcaaaacta tcaatgccgg tcgccattcg ttgggcaaca gtcgtcagag ccggcagagt
      361 atcaatactg agcaacatag tgctcagagt agcgatacca atactgaagg aaattggtac
      421 tccggctaat agcagaaaaa cgaaacttat aactagtact attaattcag tccactccat
      481 ggtattccgt ccttttttca ggtagattat ttgttgaatc gggtcgaaat agctggccaa
      541 ataaaccaat tatttccaca attgagtaaa tggcaattaa tatcccacta ataggtaaaa
      601 ccatataaac atagccaagt tttaccttca aagccgccga taattggttt aaagtcaagg
      661 taatagttac taggcgcaac ccgccaatta ccattacgga gatcgcaaaa aacagaacac
      721 aaatctggat gaatatctgc aagcggagtg cgttatttct tttcatcttg gcactgaata
      781 aatcaatcgc cagatgcatt ttatttccgg cggcatatgc ggcgccgagt aagccaaccc
      841 aaattagcag atagcgcgcc agttcttcgg tataggaact cggattacga aggataaagc
      901 gtgtaaatac ctgccagagc acattaaaca ccgagatagc cattaaaacc actacgaccc
      961 attttaaaat attatcgagt attttgcgta tttttaacat cattgcaccg ccctgattct
     1021 ttggattaat tcataaatct caggatcact tcgataactt tcgtaaatgt ccttgacttt
     1081 ttcttgaaat ggacgtttgt caggataact cacaaccacg cccgcttttt ggacttcctc
     1141 cagcgcttcc tgcgtggcaa ttttccacag ttcacgctgg tatatagcgg cttcgtccgc
     1201 cgcagactgc aaccactcct gaacttgagg tggtagatca ttccagacct tcgtgctaat
     1261 caataatata tccggcacgg cagtatgttc gtccagcgca aaatatttag ctacttcgtg
     1321 atgatgcgat aggtaaaagg agggaggatt attttccgca gcatccacaa taccttgttg
     1381 cagagccgta taaagttcgc cccaggcgat cggcgtagcc gaaccgccca tagcctgaat
     1441 catttgaatg gaagtcgtgc tttcctgtgt tcgaatcttt aaacctcgta aatcctccgg
     1501 agtattgatc ggctttttga ccgaataaaa actgcgacag ccggcatcca gataacataa
     1561 tccacgaatc cagtattttt ccccttcgag taaaagctcg cgaccgatct cgccttccag
     1621 aaccttaaac cgatgctcgt cgtcgcggaa aagatagggc atactgaaaa ctttcatttt
     1681 atccacaaaa ccttccagca cactgcacga gacctttgtc attcccagac tgccgatctg
     1741 aagcaattcc acatactcgc gttccgaacc caattgaccg ctgggataaa tatcaatacg
     1801 atattttcca ccggattttt ccgcaaagcg ttcgctcata taaaccaagg acttgtgaac
     1861 gggatgcgag gtatccagag catgcgccaa tttgatggtt ttggtgcgtg cttctgactc
     1921 acaaccattc aggcaaaagc cacttattat tattgcggta cctagtccaa ttattgttag
     1981 aaatttagtt ctcataaaat ttctcgatgt gtttagagtt tataggtttt ttttgctaaa
     2041 tcgtaggtca gcgctctgat taaaacgtgc gcttcatcta aatcaataat gtgtcgaacg
     2101 accaaaccag ctaggaaatt agaatctacc cggcgacaaa gatcgtgccg cgccggtatt
     2161 gacggaaagg aacgcgtatc atcggtaaat cccacggtat tataaaaacc ggcggtctcc
     2221 gtaaccattt cccgaaagcg aatcataccc tgaatactgt catgaaacca ccaagccgca
     2281 cccagcttca tagccggata ataaccggcg agtggcgcta actcgcggga ataggttgac
     2341 tcgtccaaag taaaaaccac cagtgtcaaa cggctatcgg taccgtattc attcagcagt
     2401 tcgcggagat taactgtgaa ctccgaacgc accggaatat cggctccggt atcaggtcca
     2461 aatcgccgaa aatttcgcaa attgtgattt cgcagagcgc caacgtgcat ctgcatcact
     2521 aaaccgtcgg ctacactcat ccgagccatc tccattaaca tattagcggt aaataaacgt
     2581 gcgtcttcgg cgctagcttt tccattacgc gcccgttgaa aaatttcttc agcctgggac
     2641 gcgctcaatc ggtgagtata ggcgctttcg actccttgat cagtcgaaac cgcgcccatt
     2701 gttttgaaaa attcgcgccg attttcgata gcggtaatga atttagagta agtattaact
     2761 tcatagccga cggcgtcccc cagtttctcg atttcttgac gccaacccac tgcatttata
     2821 ttaaataaag cgtccggccg gaagcagggt acgattcttc ctttccatcc ggattccttt
     2881 atctggcgat gatattttag gctatcagtg gcgccgtcag tggaagtcaa aacctcgata
     2941 ttaaatcgct catataaagc gcgcgggcga aactccggct tctgcagttg attttgcaaa
     3001 tgatcataaa ttgccatcgc attggcgctg ttgagctttt cggtgactcc aaaaacttca
     3061 ctaaattcat aagccagcca caaactggtg ggtgttccgg caaacagata ataattatcg
     3121 gcaaaaattt gccatatttt tcgatgatcc gtttcgaccg gcgtgccgtc gcgagtcgag
     3181 attcccagcg attccagcgg aattccttga gaatacaaaa ggcgataaat gtaatgatca
     3241 ggcaaaatga aaagagccgt cggatccgga aacggttgat tttcggctaa aattttagcc
     3301 tcgatatgcc catgcggcga gattatcggc aagtccttga cttgctggta aagttcacgt
     3361 gccagcgatc gaatcgccgg gtctgaatca aaaaaacggt cagaaggtaa aatcataact
     3421 cctcataatt gtattgtaat ttgcgacagg tttcaccaat tactaactgg gatggaacga
     3481 caatatgttg ttcgctacgt ctctccgacg aatgcaattc atccaataat agattaaagg
     3541 ctttctcacc gatggcactg gctggttgat ccataaaagt caatgctggc gacagataac
     3601 gattaaattg accaccccca aaacaaacta ggtcaatatc gccaggaatt ttcagcccca
     3661 gttcgctagc ggcttcgaaa atgccgagcg caaccggaaa agttaccgca aatatcaact
     3721 cgggtaaatc tttagtctca gctacttttt ttaatgcttc atatccggct cgctcaccaa
     3781 aaccaccttc gataatccaa tcttcgcgaa tcggcaaatt attgtcagat aatgcctgtc
     3841 gaaaaccagt ctgacgttcg tagccgatat tggtgtgact gtaaccggcg aaatgggcaa
     3901 ttttgcgata cccggcttga atgacataat tcgtcaagcg cgccgccgcc gctcggtcat
     3961 cagtcacaac gtaattactt tccaaaccga atggaatccg gtcaaaaaat acaatcggta
     4021 caccgcgctt tttgatatct tgaaaaatgc tggtgtcagt tgtattctcg gtgactgaaa
     4081 ctaataagcc ttcaactcgc atcgccagta aagtctggat gtgtttgagt tcgtattccg
     4141 gattctcctg ggatacggtc atgattatgt cgtattgact ggcattggcg gcattgtaaa
     4201 ttgattcaat gcaggtagca aaaaaatagt gagccagctt cggtacgacc aaaccaatcg
     4261 tccgtgagct tctggctgaa agattacgcg ccataaaatt gggaacgtaa cccaatttag
     4321 cggctagcgc ctggacttcg cgcttggtgg ctgagccgat atcagggtga tcccgcaacg
     4381 ccttagaaac agtgacttta gaaaccttta actggcgagc gatatcatcc agggtaacgt
     4441 ttggcgatct gtttggcggt ttgttcacta ttttttcact ttaccgttaa ctttatcggt
     4501 taagtaaaat gcaaatcttt ttccaaattg tcaagtaaat tttttatgaa tt
//
LOCUS       DAOOMU010000124         4538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_23007_length_4538_cov_4.871738, whole genome shotgun sequence.
ACCESSION   DAOOMU010000124 DAOOMU010000000
VERSION     DAOOMU010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23007_length_4538_cov_4.871738"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1819
                     /gene="cas10"
                     /locus_tag="PLI55_09260"
     CDS             173..1819
                     /gene="cas10"
                     /locus_tag="PLI55_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HPB01058.1"
                     /translation="MNDNTLSYAGFTIGPIYDVMKHARKTREMWFGSYFFSWYMEVLE
                     RELQKNKKIEFLTPFIDNKENDRRAGLYPDRFVLESSLSPEEIRNTIKTANEKALKHF
                     IQIINNLGSLPAITFFKKKEIGNILREYLQTNMVVLPAEKIAGKNVIETFWDYLDATE
                     EHRHFKLGKSEKTCERCHTLLSVATAKVMDNNKKKDFDLCPLCLLKLFTLEVVDVQNK
                     ISGWHRYPSTGEISAVELVEPFRKNDGTYQDELQNYFDKHDEIEFDDIEFRQIVNKEI
                     KPYHKYIAILDADGDKIGELAKKVKDPTAFSEMLFNFGKESYEICSDYSRSGKGIGEP
                     IYIGGDDVLAFLPVAYRKSDKDITTIIDLAMDLNKAFKVQMKSTEVGFSIGISIAYYK
                     HPLAVTLNKSREMLKIAKAQDGKDTLAIHLKKHSGNDAQVVFSFKESERLKEFNGLLK
                     AVLLSEEDLMGSIHYNLLRFKNLLIQIETKEQLTNFFDNNFSDQKKAGYTGIDHVQNM
                     FEMALGLNSVAIPANSREKAVNEVLDQLWFIKFLVGKEEE"
     gene            1822..2967
                     /locus_tag="PLI55_09265"
     CDS             1822..2967
                     /locus_tag="PLI55_09265"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HPB01059.1"
                     /translation="MQYLITFKPYSTFFFGTFKSFGEGYYAESSRFPLQTSIVGCLRK
                     TILKQNGWLDQKGNYPDKKYRNQYEQITGSVATVSMDSSDLNLGIIEKVSPVFIVRQD
                     PGHFCPDDFLFPVPQDIDYRYDPVEKDEEGNKLIIGFDYLAYQNESNAEEGSEIVTTK
                     IRNPKNDPPDYLGDRDFWENYVRHEKIPYHPGCESGNIFLNVVQQGIARDENRQTVEG
                     AFYEKHAFCLAKGYSFGVIAVLNGETPFKKEKDDVFLGGDRSLFQMQIKPITAPYELR
                     YTGHPVMNRILGSEPHYEPPDDDNKYVCISALYGDQEPLDLEHALILSLEANRFLEYQ
                     KKKTDSYCGIPHSSVLYPKKGKSFIYKGYDMATKIGYNFIIKAKKGF"
     gene            2969..3835
                     /gene="cmr4"
                     /locus_tag="PLI55_09270"
     CDS             2969..3835
                     /gene="cmr4"
                     /locus_tag="PLI55_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HPB01060.1"
                     /translation="MYTYQAYLIETLTNLHVGSGGEQIGIVDNQIQRDPVTGFPSIYA
                     SSLKGSLRDHVKKNSNDSDKNTVIDIFGGSLDLEADEKKKQRTLEPGKVVFFDAHLLT
                     LPFRSSQKVYYNCTCPEILDNFTEQVETFNIPIKQTGKPSFGNDTSDFRVFDLVNPNL
                     EIEDFSVGNLTPGSENIDSFLTPVAGIIKTNFALFQNNIFTEICKTGLPVIARNSLDE
                     NGISQNLWYEEFLPRRTRLWFILGFPEDYKNKKWLEERICADKIVQIGANFSIGYGYC
                     RIHKLPFPEVQS"
     gene            3832..4299
                     /locus_tag="PLI55_09275"
     CDS             3832..4299
                     /locus_tag="PLI55_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01061.1"
                     /translation="MNRKNIEDWIPIAIEITQQWRSEKLKHPDKLSNDEEKQWELGIP
                     SKYFGYLDSFGPTILQSGIINSLTFYCQESGGADRIEIANLIRDVLISSNTLTRIPNP
                     NLSLLELTQDKISQDRFYWRNRIFEAIIACKMALKLFHRAKPEDVQSKDGEAV"
     gene            4296..>4538
                     /locus_tag="PLI55_09280"
     CDS             4296..>4538
                     /locus_tag="PLI55_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01062.1"
                     /translation="MTTNAPHVFYISKAEKNGQDIDQRTIGELLGTNIKDVMVNNINY
                     EDYYQIPGAVSFPLKTIYPGLLIGAGYTHTYKSVKKS"
BASE COUNT         1480 a          866 c          959 g         1233 t
ORIGIN      
        1 aggatcataa agatgatagt aaggtaactt ggaaggtgat aataccgact attcctgatt
       61 ttgatctaaa cgattttatt aagtgggcat gttctgaaat caataaggat aatactaatg
      121 ttatttttcc aaaatttgaa aatatttgat ctgaatggaa aagaggtgat gtatgaatga
      181 taatacttta tcctacgccg gtttcactat cggcccaatc tatgacgtca tgaaacatgc
      241 ccgtaagacc cgcgagatgt ggtttggttc gtattttttc tcgtggtata tggaagtgtt
      301 agaaagagaa ttacaaaaga ataaaaaaat tgaatttctg actccattca ttgataacaa
      361 agaaaatgac cgcagagccg gactctaccc ggaccggttt gtattggaat catcattatc
      421 accggaagaa atacgaaata caattaaaac ggccaatgaa aaagctctca aacattttat
      481 tcaaattata aacaacctcg gtagtctgcc tgcgataact tttttcaaaa aaaaggaaat
      541 cggcaatatt ctgcgggaat atctccaaac caatatggtt gtccttccgg ctgaaaagat
      601 agccggaaaa aatgtcattg agacattttg ggattactta gacgcaaccg aagaacatcg
      661 ccatttcaaa ctcggtaaaa gtgaaaaaac ctgcgagcga tgtcataccc tactatcagt
      721 cgctactgcg aaggtgatgg ataataacaa aaaaaaagac ttcgatttat gtccgctatg
      781 tctgctaaag ttatttactt tagaggttgt tgacgttcag aataaaatct cgggatggca
      841 tcgatatcca tctaccggtg aaatcagcgc ggtggaactg gtcgaacctt ttcgaaaaaa
      901 tgacggtaca tatcaagatg aattgcaaaa ttactttgac aagcatgatg aaattgagtt
      961 tgacgatatt gaatttagac aaatcgttaa taaggaaatc aaaccctatc ataaatatat
     1021 tgcaattctg gatgccgatg gcgataaaat aggtgaatta gcaaaaaaag taaaagatcc
     1081 aacagcattt tcagaaatgt tattcaactt tgggaaagag tcttatgaaa tctgttcaga
     1141 ttattcaaga tcaggaaagg gaataggcga gccgatctat atcggtggcg acgatgtcct
     1201 ggcatttttg ccagttgcat atcgaaaatc tgataaggat attacgacta taattgatct
     1261 tgcaatggat ctgaataaag cctttaaagt acaaatgaaa tcaacagagg ttggttttag
     1321 catcggcatc agcatcgcct attacaaaca cccgctggct gttacactca ataagtcgcg
     1381 ggagatgtta aagatcgcta aagcgcagga tggcaaggat acactggcta tccaccttaa
     1441 aaaacactcc ggcaatgacg cgcaagtagt gttttcattt aaggaatccg aaagactcaa
     1501 agaatttaat ggattattaa aagcagtatt actttctgaa gaagacctga tgggttcgat
     1561 tcactacaat ctgctgaggt ttaaaaatct gctgatccag atcgaaacga aggaacagtt
     1621 aacgaatttc ttcgataata atttcagtga ccagaaaaaa gcaggttata ccggaattga
     1681 ccatgttcaa aacatgttcg aaatggcgct gggactaaat agtgtcgcta tcccagcgaa
     1741 cagtcgtgaa aaagctgtca atgaagtact tgatcaactt tggtttatca agtttttagt
     1801 cggcaaggag gaggaatgaa aatgcaatat ctgattactt tcaagccgta cagtaccttt
     1861 ttcttcggga cgttcaaatc atttggcgaa ggttattatg ccgaatcgag ccggtttcca
     1921 ctccagacca gtatcgtcgg ttgcctgcga aaaactatcc tgaaacaaaa cgggtggttg
     1981 gatcagaaag gcaactaccc ggacaagaaa taccgaaatc aatatgaaca aataaccggg
     2041 agtgttgcaa cagtttctat ggattcgtct gatctgaatt tgggaattat tgaaaaagtc
     2101 tcacccgtct ttatcgttcg gcaggacccc ggacatttct gcccggacga ctttctgttt
     2161 ccggttccac aagatattga ttatcgctac gatccagtcg aaaaggatga agagggaaac
     2221 aaattaatta ttggttttga ttacctggca taccaaaatg aatcgaatgc agaagaagga
     2281 tcagaaatag tcacaacgaa gatccgcaat ccgaaaaacg atccgcctga ttatcttggt
     2341 gaccgggatt tctgggaaaa ctatgttcgc catgaaaaaa ttccttatca tcccggttgt
     2401 gaatcgggca atatcttttt aaatgtcgtt cagcagggaa tcgcccgtga tgagaaccgg
     2461 caaactgttg agggtgcctt ctatgaaaaa catgccttct gtctggcgaa aggctattct
     2521 tttggagtga ttgccgtgct taacggagaa accccattta agaaagaaaa agacgatgtc
     2581 tttttgggcg gcgaccgatc gttgttccaa atgcaaatta agccgattac cgctccttat
     2641 gaactaagat atactggtca tccggttatg aatcggattc tgggatcaga accacactat
     2701 gaaccacctg acgacgataa taaatatgtc tgtatctcgg ctttgtatgg agatcaggaa
     2761 ccgctggatt tggaacatgc gttgatcctt tctctggaag ccaatcgttt tcttgaatat
     2821 caaaaaaaga aaaccgattc ctactgcggt attcctcata gttcggttct atatcctaaa
     2881 aaaggaaaat catttattta taaaggctac gatatggcta cgaagatagg atataatttt
     2941 ataataaaag cgaagaaagg attttaatat gtacacatat caagcttatc tcattgaaac
     3001 ccttaccaac ctgcacgtcg gtagcggcgg ggaacagatc ggtattgtgg ataaccaaat
     3061 tcagcgcgat ccggttactg gttttccatc aatatatgct tcgagtctaa aaggctcgct
     3121 cagagatcat gttaagaaga attctaatga ctctgacaaa aacactgtta ttgatatttt
     3181 cgggggctct ttagaccttg aggcagacga aaaaaagaag caacgcactc ttgaacccgg
     3241 caaagtcgtt tttttcgatg cgcatctgtt aaccttgcct ttccggtctt ctcaaaaagt
     3301 atattataac tgcacctgtc cggaaatcct ggataatttt accgagcagg tggaaacttt
     3361 taatattccg ataaaacaaa ccgggaagcc atctttcggg aatgacacat cggattttcg
     3421 ggtgttcgat ttggttaatc cgaatttgga gatagaagat ttcagtgtcg gaaatctgac
     3481 tccgggtagt gaaaatatag atagtttttt aacgccagtt gccggaataa tcaaaactaa
     3541 ttttgcgctt tttcagaata atatttttac cgaaatctgt aaaaccggtt tgccggttat
     3601 cgcccgtaac agcctggatg aaaacgggat cagtcagaat ctgtggtatg aggagttcct
     3661 gccacgaaga acccggctgt ggtttatttt aggatttcct gaggattata aaaataaaaa
     3721 atggctggag gaaagaatct gcgccgataa aatcgtccag atcggagcca atttctccat
     3781 cggttatgga tattgccgga ttcataaact accttttccg gaggtgcaat catgaaccgg
     3841 aaaaacatcg aagactggat tccgatcgcc attgaaataa cccagcaatg gcgaagtgaa
     3901 aaattaaagc atccggataa attatcgaat gacgaggaaa aacagtggga attaggaata
     3961 ccgtccaaat acttcggcta cctcgattca ttcggaccga ccatattaca atcgggcatt
     4021 atcaattccc tgacatttta ttgccaggaa agtggcggag cggataggat cgagatcgcc
     4081 aatcttattc gcgatgtttt aattagtagc aacactttaa ccagaatacc aaatccgaat
     4141 ctgtctcttt tggaactaac ccaagataaa ataagccagg atcgctttta ctggcgcaac
     4201 cggattttcg aagcgatcat tgcctgtaag atggccctta aattatttca tcgagcaaaa
     4261 ccagaagacg tgcaaagcaa agacggggag gctgtatgac aaccaatgcc ccgcatgttt
     4321 tttacatttc aaaagcggag aaaaacggcc aggacattga tcagagaacg atcggcgaat
     4381 tgctgggaac aaatataaaa gacgtaatgg tcaacaacat taattatgaa gattactacc
     4441 agattccggg cgccgtttca ttccccttaa aaaccattta tcccggatta ttgatcgggg
     4501 cgggttatac tcatacctat aaatctgtaa aaaaatcc
//
LOCUS       DAOOMU010000125         4536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_23018_length_4536_cov_4.514840, whole genome shotgun sequence.
ACCESSION   DAOOMU010000125 DAOOMU010000000
VERSION     DAOOMU010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23018_length_4536_cov_4.514840"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..3788
                     /locus_tag="PLI55_09285"
     CDS             393..3788
                     /locus_tag="PLI55_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPB01063.1"
                     /translation="MSNFITNQPTKELQKRLSELITHSDELKFLVGFFYFSGIRELYD
                     GLKANDRVIIKVLVGLNVDLYNYKLVEFASQEQLNDAEKANQFFQSVKNSINTEYFDN
                     KEFYEQVRFFIELIQEDRLLIRKTDYPNHAKLYIFKLEEGQVGKKELFITGSSNLTRA
                     GLTTQNEFNVEISDYGFAEADRYFDTLWNKAVKITESAEYKTKLIEVIKKETLVKEIT
                     PFEAYCLVLKSYLDCFTHKEIGTAIIEILEKNGYKSYQYQLDAVREAISIIENNNGVL
                     IADVVGLGKSIIACAVALHLRERGLVICPPGIIGDVNKKSGWRMYIEQFGLFGWEVRS
                     LGDLENTLNFVKEHDDIKVIIVDEAHRFRNQDTESYDLLKNICRNRKVILLTATPFNN
                     KPEDVLSILSLFITPKKSSITLSNNLTEIFRHFDQLFKKLSFITKNHNSQDPKKKAKV
                     QLYYKSLFDEDTIDLKKIKKRTHMIARQIRNVIEPVTIRRNRLDLEKNPYYKNEVKNL
                     SKVADPIEWFYELTPEQSAFYDKIIQDYFGDSNNGRKFHGAIYRPFEYEEGKMEDVEE
                     GKLSLEKQREYIVQRALFDIIRRLLVKRFESSFGAFQQSITNFKNFHTDVLDFITKMG
                     NGDPLKGEYILDRDLLEKIFAMDVDEIEEHLKEYEEKIKRGEYPKKHRRYKIAKFKYA
                     NEFVNDIRSDIEMFAEILETLNALNLVNDDPKTKCLIKNIQSKFAEPATAGEPKRKIV
                     IFSEYADTVKYIGNDLCRCYPDRVLVVYGKLSDAKIEVINRNFDASYKKPSDEYDILL
                     STDKLSEGFNLNRAGMIINYDIPWNPVRVIQRLGRINRISKKVFDTLYIVNFFPTEKG
                     AEYVKSREIAQTKMFMIHNTLGEDSKIFDIDEEPSPAALYQKINQNPDEMESESFYTR
                     VINLFNEIQKQYPDVIQNLNDAPRRIKVAKNFHQDELIVFFKKNHFYVKTAKISDPNL
                     EITDSSLEEVLEKIQCQPETPSLPISEQFWDSYEAVKFSLGNKKALSKMPNIEREAFN
                     NIEFLLKFNKSELLTESKSFLRTLREDILDYGTLPDYTLRRIANLKTANHSIEKIIAE
                     IEKLRAELGADYLDKEKARLKNLDREIIIAIHNQNLNDLQKVG"
     gene            3791..>4536
                     /locus_tag="PLI55_09290"
     CDS             3791..>4536
                     /locus_tag="PLI55_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01064.1"
                     /translation="MTNNEILLDFINDFKPAKVNPLFRELSRGTLRPYEEHLPQYTSA
                     PFSDGYWHGAMQLTDENELIVCSFMIHKELSERSGKKEQYNLAKKILKDFQRYTGGFF
                     IFYDTKGDFRFSLIYDIPGPDGKRYGNNFRRYTYFVSKEQTNKTFRTRLSEADFSYFE
                     NIRDAFSVEKVTKAFYSELQNWYFWAMNIVEFPDDEDKDKNSRNAKNLIRLITRIIFI
                     WFMKEKRLIPSSLFDKQFIDNLLNYNDKTG"
BASE COUNT         1509 a          913 c          883 g         1231 t
ORIGIN      
        1 ctgacttacc gcctttatcc gacgctatta tgcagaaaat ccaggaaatt tacgagcacg
       61 atgtgcgccc ccatgtgcat caatactggt aaaaagttac tataataaaa gcgcggaatg
      121 agcatctatt gaggagtaga ttttctatcc ctgttgctct ataatactct tactactacg
      181 cggcgggtgc taacttagga acgcatttat tcctaatagg aacgtagttt ttgtgacttt
      241 ggagtagaat ataaccgtca gtagttccta cttcgcttag agtaaacatt tcaaaaatta
      301 gagggactgc tgaggataaa aaggttgcat ttatggctaa atctaaataa cattgacaaa
      361 attgatttaa caataaaggc aaggataagg ggatgtcgaa ttttatcact aatcagccta
      421 cgaaagaact acaaaaacgc ctgagtgaac ttattaccca tagcgacgaa ctcaagtttt
      481 tagtagggtt tttctatttt tcgggtattc gtgaattgta cgatgggctt aaggcaaatg
      541 acagagtcat tatcaaagtt ttagtgggat taaatgttga tctttataac tacaagttgg
      601 ttgagtttgc atctcaggaa caactcaacg atgccgaaaa agcgaaccag ttttttcaat
      661 cagtcaaaaa ttccattaat accgaatatt tcgataataa ggagttctac gaacaggtgc
      721 gattctttat cgaactaatt caggaagatc gcttgctaat ccgcaaaact gattacccca
      781 accatgccaa actttatatc ttcaaactcg aagaagggca ggtaggtaaa aaagaactat
      841 ttattaccgg aagtagcaat ctgaccagag ctgggctgac tactcagaac gaatttaatg
      901 tcgagattag cgactacggt ttcgccgaag ccgatagata ttttgatact ttatggaata
      961 aggcggtcaa aattactgaa tccgctgaat acaaaactaa gctcatcgag gtcatcaaaa
     1021 aagagactct agttaaagaa attacaccat tcgaagctta ttgtctggta ctcaaatctt
     1081 accttgactg ttttacgcat aaagagatcg gcacggcgat tattgaaatt ttggaaaaaa
     1141 acggctataa atcttatcaa taccagttgg acgcggttcg ggaagccatc agcattattg
     1201 agaataataa cggagtgttg attgccgatg tggttggact gggaaaatcc atcatcgcct
     1261 gcgcggtcgc tctacactta cgtgaacggg gattggttat ctgcccaccg gggattattg
     1321 gcgacgttaa taaaaaatcc ggctggcgaa tgtatatcga gcagttcgga ttattcggct
     1381 gggaagtgcg ttctttaggt gatttggaaa acacactcaa tttcgtcaaa gaacatgatg
     1441 atattaaagt gataattgtt gatgaagccc atcgttttcg caatcaggat acggaaagtt
     1501 atgatctgct gaaaaatatt tgccgcaatc gtaaggtgat tttattaacc gctaccccgt
     1561 tcaataataa gccggaagat gttctttcta tcttgtcatt gttcataaca ccgaaaaagt
     1621 catctattac acttagtaat aatctgactg aaatattcag gcattttgat cagcttttta
     1681 agaagttgag ttttattacc aaaaatcata attcccaaga cccgaagaaa aaagcaaaag
     1741 tccaattata ctataaatct ctattcgatg aggatacaat agatttaaaa aagatcaaga
     1801 agcgaaccca tatgatagcc agacaaattc gtaatgtcat cgaaccagtt actatccgac
     1861 gcaatcggct cgacttagag aaaaaccctt attacaaaaa cgaagtcaag aacctatcta
     1921 aagttgctga cccaatcgaa tggttctacg aattaacacc ggaacaatcg gctttttatg
     1981 ataaaatcat tcaagactat ttcggagact cgaataacgg cagaaaattc cacggcgcca
     2041 tttaccgccc atttgaatat gaagaaggca aaatggagga tgtcgaagaa ggcaaattaa
     2101 gcctggaaaa acaacgagaa tacatcgttc aacgcgcctt attcgatatt atccgtcgtc
     2161 tgttggtcaa gcgctttgaa agttcattcg gtgcattcca acaaagtata actaatttta
     2221 aaaattttca taccgatgtg cttgacttta ttaccaaaat gggaaatggc gatcctctca
     2281 aaggcgaata tatcttggac cgagacctgc tcgaaaaaat ctttgcgatg gatgtagatg
     2341 aaatcgaaga acatctcaag gaatatgagg aaaaaatcaa acgtggtgaa tatcctaaaa
     2401 aacacaggcg ttataaaata gccaagttta aatatgccaa tgaatttgtt aatgatatcc
     2461 gtagtgacat cgaaatgttt gctgaaatcc tcgagactct gaatgcattg aatctggtca
     2521 atgatgatcc taagaccaaa tgcttgatta aaaatattca atcaaaattc gccgaaccgg
     2581 ctacggcggg cgaacctaag cgcaaaatcg tgattttttc cgaatacgcc gataccgtta
     2641 aatatatcgg gaatgatctc tgccgctgtt atcccgatcg cgtgctggtc gtttatggca
     2701 aattgtccga tgccaaaata gaagttatta accgtaactt cgacgcttcc tataaaaaac
     2761 caagtgatga atacgatatt ttactcagta ctgataaatt gtcggaaggc ttcaacctga
     2821 atcgcgccgg aatgatcatt aactacgata ttccttggaa cccagtccga gtgattcaac
     2881 gtctgggacg aatcaaccgg atcagtaaga aagtgtttga taccctgtac atcgttaatt
     2941 ttttcccaac ggaaaagggc gctgaatacg tcaagtcgcg cgaaattgct caaaccaaga
     3001 tgtttatgat tcataatacc ttaggagaag attccaagat tttcgatatt gacgaagaac
     3061 cttctccggc ggctctttat caaaaaatca accagaatcc cgatgaaatg gaaagcgaaa
     3121 gcttctatac ccgcgtaatc aatcttttca atgaaatcca aaagcaatat ccggacgtca
     3181 ttcagaatct caatgatgct cccagaagaa tcaaagtcgc caaaaatttc catcaagacg
     3241 aactaattgt ctttttcaaa aagaaccatt tttacgtcaa aaccgctaaa attagcgatc
     3301 ctaaccttga aattaccgac tcgtccctcg aggaagtttt agaaaaaatt caatgtcagc
     3361 ccgagacgcc ttctttaccg atttcggaac aattctggga ttcctatgag gctgtaaaat
     3421 tttctttagg caataaaaaa gctctttcca aaatgccaaa tatcgagaga gaagcattca
     3481 acaatatcga atttttactg aaattcaata aatccgaact gctgaccgaa tccaaaagtt
     3541 ttctgcgaac cctgcgcgag gacattctcg attatggcac tctgccggat tataccctgc
     3601 gtcggattgc caatctgaaa actgccaacc atagtatcga gaaaattatc gccgagatcg
     3661 aaaaactgcg cgctgaactg ggcgcagatt atcttgacaa agaaaaagcg cgcctgaaaa
     3721 atctggatcg cgaaattatt atcgccattc ataatcagaa cttaaatgat cttcagaaag
     3781 ttggttaaat atgacaaaca atgaaattct gctcgacttt atcaatgact tcaagcccgc
     3841 aaaagtcaat cccctctttc gcgaattaag tcgaggaact ttgcgaccat acgaagagca
     3901 tttaccgcaa tatacatcgg cgcccttttc ggatggctat tggcacggcg caatgcaact
     3961 caccgacgaa aatgaactca tcgtctgcag ttttatgatt cataaagagc tgagcgagcg
     4021 cagtggcaag aaagaacagt acaatttagc taaaaaaata ctcaaagatt ttcagcgcta
     4081 taccggcgga ttttttatct tttacgacac gaaaggcgat tttcgcttct cgctgattta
     4141 tgatattccc ggtccggacg gcaaacgcta tgggaataac ttccgccgct atacctattt
     4201 tgtaagcaaa gagcagacca ataaaacatt tcgcactcgc ttaagcgagg cagatttctc
     4261 gtattttgaa aatatcagag acgccttcag cgtagaaaag gtaacaaagg cgttctattc
     4321 tgaattgcag aactggtact tctgggcaat gaacatagtt gaatttcccg atgatgagga
     4381 taaagacaaa aacagccgca atgcaaagaa tcttatccgg ctgataacac gcatcatctt
     4441 tatctggttt atgaaagaaa aacggcttat tccatcgtcg cttttcgata aacaatttat
     4501 tgataacctc ctcaattaca acgataaaac cggcag
//
LOCUS       DAOOMU010000126         4532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_23046_length_4532_cov_24.400045, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000126 DAOOMU010000000
VERSION     DAOOMU010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23046_length_4532_cov_24.400045"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1497)
                     /locus_tag="PLI55_09295"
     CDS             complement(16..1497)
                     /locus_tag="PLI55_09295"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPB01065.1"
                     /translation="MGKQVSNEQMDKLVSLCKRRGIIFQSSDIYGGLSSTWDYGPLGV
                     ELKRHVKDLWWHDMVTSRENVVGMDAAILMHPKVWEASGHVANFHDPLIDCKKCQARF
                     REDKAPAYFKCSNCNFNFYVNDGLIEVKKEFAAHFPDLYAREIAHYRDSDCPHCGGQR
                     TIKLEKIACPNCGGSELSPARQFNLMFKTHFGPSEDSASEVYLRPETAQGIFVNFDNI
                     ISTTRVKLPFGVGQIGKAFRNEITTGNFIFRSREFEQMEMEFFVKPEESGRWYEYWVN
                     ERFNWYKKLGVRAENLRRRPHEKEELAHYAKACTDVEYKFPFGWSELEGIADRQTFDL
                     EQHMKASGKKLTYFDSETNTHIVPAVVESSAGVDRTILTVLCDAYWEDAEHDRVVLRL
                     DPKIAPTKVAILPLVNRDGMPEIAEAIFNDVKKQFAAFYDQAGSIGRRYRRQDEAGTP
                     FGITVDSQTLQDGSVTLRDRDTLEQIRLPKENIVKVLQEKLAL"
     gene            complement(1579..3786)
                     /gene="topA"
                     /locus_tag="PLI55_09300"
     CDS             complement(1579..3786)
                     /gene="topA"
                     /locus_tag="PLI55_09300"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPB01066.1"
                     /translation="MELLIVESPSKAKTIKKYLGNNFKVMASVGHIKDLPEKELGIDI
                     QHDFKPKYVTIKGKSKIISNLKESAQSAQRVYLAPDPDREGEAIAWHIANVIDVPSDR
                     VQRVLFNEITESGIRYGLSHPRQIDMSLVNAQQARRVLDRLVGYQVSPFLWRTLYSGL
                     SAGRVQSVAVRIICEREVEIEQFIPLEYWEITALLQTPQNEKFSAKLIKIDNKKTAIS
                     NASDANKHLAILKNATYQVSNIEEKEVTKSPAPPFTTSTLQQEATRRFRYSPEKTMRI
                     AQNLYEGVEIGGEPVGLITYMRTDSVRISKEAITGIREYIEKHYGAKFLPPNPRHFKT
                     TKRNVQDAHEGIRPTHFEYTPEVVAPYLSPEQKKLYTLIWNRFAACQMSAAVYKQKII
                     DVTADNYLFRATGSDLIFDGYLRAWREEKAEDEKKIALPINIQKDDRLTLLELQPEQK
                     FTEPPPRFTEGTLIKELDNLGIGRPSTYATIVSTIITRKYVERKGGYLVPTDLGKTVN
                     KLLVDGMPDIFNVKFTARMEAELDDIEGNRKEWQKVISEFYQPFHKSLEELQAQRREI
                     KTGLQEKTNEKCEICGAPMVIKWSRNGRFLACSAFPECKNTRPLNTAPVAPTESKTCP
                     NCGSPMTIKDGRFGKFWACTKYPKCKTTEPFTLNIKCPEPDCDGQIVQKRTKKGKIFY
                     GCNRYPNCKFATWNEPVARPCQNCDSQFLERKIKRDGSVLLICPKCKTETADE"
     gene            4342..4415
                     /locus_tag="PLI55_09305"
     tRNA            4342..4415
                     /locus_tag="PLI55_09305"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4376..4378,aa:His,seq:gtg)
     gene            4429..4503
                     /locus_tag="PLI55_09310"
     tRNA            4429..4503
                     /locus_tag="PLI55_09310"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4462..4464,aa:Glu,seq:ttc)
BASE COUNT         1156 a          995 c         1035 g         1346 t
ORIGIN      
        1 aacctcgacg gttccttaca acgctaattt ttcctgcagt accttgacaa tattctcttt
       61 gggaagacgg atctgctcga gtgtatcgcg atcgcgcaat gttacactgc cgtcttgtaa
      121 ggtctgcgaa tccacggtaa ttccaaacgg cgtcccggct tcatcctggc gccgatagcg
      181 tcgtccaatg gaaccggctt ggtcataaaa agccgcaaac tgttttttta catcgttaaa
      241 aatggcttcc gcgatttccg gcattccatc acgattgacg agcggtagga tagcgacttt
      301 ggtcggagct attttcggat cgagtcgcaa gacgacgcgg tcatgctcag catcttccca
      361 gtaagcatca cacaaaaccg tcagaatggt gcgatctacg ccagcagaac tttcgacaac
      421 ggctggtacg atatgcgtgt tcgtttcgct atcaaaataa gtcagcttct tgccgctggc
      481 tttcatgtgt tgctcaaggt cgaaagtttg ccgatcggcg atgccttcca gttccgacca
      541 gccgaaggga aatttatatt caacatcagt acaggctttg gcataatggg ccagttcttc
      601 tttttcgtgc ggacgccgcc gcaaattttc cgcacggaca ccgagttttt tataccagtt
      661 gaagcgttca ttgacccaat actcgtacca gcgcccgctc tcttccggtt tgacaaaaaa
      721 ttccatttcc atttgttcaa attcgcggga gcgaaaaatg aaattgccgg tcgtgatttc
      781 attgcgaaaa gcctttccga tctgaccgac accaaacggt agtttgacac gcgtagtgga
      841 aatgatatta tcgaaattga caaaaatgcc ctgcgccgtt tccggccgta aatagacttc
      901 agaagcagaa tcttccgatg gaccaaagtg ggttttgaac attaaattga attggcgggc
      961 gggtgatagt tccgaaccgc cgcagttcgg gcaggcgatt ttttccaatt tgatggttct
     1021 ctggccgccg caatggggac agtcggaatc gcgataatgc gctatttcgc gagcgtataa
     1081 atcgggaaaa tgcgccgcga attccttttt tacttcaatt aaaccatcat tgacgtaaaa
     1141 gttaaaatta caattggagc atttaaaata ggctggagct ttatcctccc ggaaacgcgc
     1201 ctgacatttc ttgcaatcaa tcagtgggtc atgaaaattg gcgacgtgcc cagaggcttc
     1261 ccagactttg gggtgcatca atatagcggc gtccataccg acgacatttt cccgactggt
     1321 aaccatatca tgccaccaca aatccttgac atgacgctta agttcgacgc cgagcgggcc
     1381 ataatcccag gtactgctca gcccgccata aatatcactg gattgaaaga tgatgccgcg
     1441 ccgtttacat agcgagacta atttatccat ttgttcgtta ctaacttgtt ttcccatttt
     1501 ttccctaatt ttcacatttc aatatttaat ttacaaatag ttcagaagga aatttctaat
     1561 taagcaacat gtgatttttc actcgtctgc tgtttcagtt ttgcattttg ggcaaatcag
     1621 cagaacactg ccatcgcgtt taatcttgcg ttccaaaaat tggctatcgc agttctggca
     1681 ggggcgagcc accggttcat tccaggtagc gaacttgcag ttcgggtagc gattacatcc
     1741 gtaaaaaatt ttgccttttt tggtgcgctt ttgaacgatt tgcccgtcgc aatctggttc
     1801 cgggcatttg atattcaaag tgaatggttc ggtcgtttta catttaggat atttggtgca
     1861 agcccagaat ttgccgaaac gcccatcttt gatcgtcatc ggactgccgc agttgggaca
     1921 ggttttggat tcggtcggcg caaccggcgc ggtatttaac ggccgggtgt ttttacattc
     1981 gggaaaagcc gagcaggcta aaaaacgccc attgcgactc catttgatga ccatcggagc
     2041 gccgcagatc tcgcattttt cattagtttt ttcctggagg ccggttttga tttcgcggcg
     2101 ctgggcttgg agctcttcga gcgatttatg aaaaggctga tagaattcgc tgatgacctt
     2161 ttgccattct ttacgattgc cttcaatgtc atccagttcg gcttccattc gagcagtgaa
     2221 cttgacattg aaaatatcag gcataccgtc cacgagtaat ttattgacag ttttgcctag
     2281 atcggttggc accagatagc cacctttacg ttcaacgtat ttacgggtaa taattgtcga
     2341 aacgatggtg gcataagtgc ttgggcgacc gataccgaga ttatctaact ccttgataag
     2401 cgtaccttcg gtaaagcggg gcggcggttc ggtaaatttt tgctccggtt ggagctctaa
     2461 caaggtgagc cggtcgtctt tttgtatatt gatcggcaga gcaatttttt tctcgtcctc
     2521 ggctttttcc tcgcgccagg cacgcagata gccgtcaaaa atcaaatcac tgccagtagc
     2581 gcgaaaaagg taattatcgg ctgtcacatc tataattttt tgtttgtaaa cagccgcaga
     2641 catctgacag gcggcgaagc gattccaaat tagagtataa agctttttct gctcgggcga
     2701 aaggtaggga gcaactactt cgggcgtgta ttcaaaatgc gtggggcgaa ttccttcatg
     2761 cgcgtcttgc acgtttcttt tagtagtttt aaaatggcgc gggttaggtg gaagaaactt
     2821 tgcgccgtaa tgtttttcga tatattcacg gataccggtg atagcttcct tagaaatgcg
     2881 taccgaatca gtgcgcatgt aggttatcaa gccgaccggc tcaccaccaa tttcgacacc
     2941 ttcgtaaaga ttctgagcaa tgcgcatagt tttttccggc gagtagcgaa aacggcgcgt
     3001 ggcttcctgt tgcagggtgc tggtcgtgaa aggtggcgcg ggtgatttgg tgacttcttt
     3061 ctcttcgata ttgctgactt gataggtagc atttttcaga atcgctaaat gcttgttggc
     3121 gtcggatgcg ttcgaaatag cagttttttt attgtcaatt ttaatgagct tggcagaaaa
     3181 tttttcgttt tggggtgtct ggagcagtgc cgttatttcc cagtattcaa gaggaatgaa
     3241 ctgctcgatt tcgacttcgc gttcacaaat gatacgcacc gcgacggact gcacacgacc
     3301 ggcgctcaat ccactataaa gtgtgcgcca tagaaaaggg ctgacttggt aaccgaccag
     3361 ccgatcgagg acccgtcggg cttgctgggc attcaccagg ctcatatcta tctggcgcgg
     3421 atggctaagt ccataacgaa tgccactttc ggttatttca ttgaaaagaa ctcgttgaac
     3481 gcgatcggat ggaacgtcaa taacattggc tatatgccag gcaattgctt caccttcgcg
     3541 gtctggatca ggggctaaat aaacacgttg agcagattga gcggactctt tcagattact
     3601 tataatttta gatttgcctt tgattgtaac gtatttcggt ttgaaatcgt gttgaatatc
     3661 aataccaagt tctttctccg gtaaatcttt gatgtgacca accgatgcca tgactttaaa
     3721 attgttaccg aggtattttt tgatagtctt ggctttagaa ggcgattcga cgattagtaa
     3781 ttccatatca ctttactttt cttaaggcgg ggtaaattac aaatactgat cagaattcca
     3841 agcattttta aaataaaact tgttcatttc aaaaatactc actgatattg agacttccac
     3901 aaattttaag aaaaataccc gcgctttttg attatcagat tagttttcgc ctcatcatcc
     3961 aattttgttg aatcacgaaa aaaagatgtt tatttccacc gctgaaagta ataatagtga
     4021 attcagaaat accgaatcct gttttaggaa aaggctaaaa tatgaacgca caaaaactga
     4081 cctgcacgtt ttaagatagg taagcacaat agattttaat aatttttaaa aaaatgtaat
     4141 ttaatatcca gttaaagtaa tatcattctg attatcagca tattagcaga taagttcggg
     4201 aaatattagg ggatatcctg gtgcttggtg tattgacatt ctgattataa ataacttaaa
     4261 tttcacagct tttttaaaat aattgatcca ttcatgaaat tgatctgggc ttactaacct
     4321 cctgcgggag tgatatagat ggtgagtgta gctcagtatg gtagagcgac aggttgtggc
     4381 cctgttggcc gcgggttcaa gtcccgtcac tcacccgctc tttttaatgg cgctatcgtc
     4441 tagtggttag gacaccacgc tttcaatgtg gtaaccggag ttcaattctc cgtagcgcta
     4501 ccaatcgttc caagtctttt ctttagcctt ta
//
LOCUS       DAOOMU010000127         4483 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_23376_length_4483_cov_16.763324, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000127 DAOOMU010000000
VERSION     DAOOMU010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23376_length_4483_cov_16.763324"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            258..1889
                     /gene="pruA"
                     /locus_tag="PLI55_09315"
     CDS             258..1889
                     /gene="pruA"
                     /locus_tag="PLI55_09315"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPB01067.1"
                     /translation="MSNAKFTLPQPVNEPQFSYLPGSSERQALKIALNKIAGEQIEIP
                     LIIGGKEIRTGQTAKCIMPHNHQHILAIYHQATAKEVSLAVEASQKAWEFWSVMPWHE
                     RAAIFKKAAALLSGPWRMTLNAATMLNQSKNVFQAEIDSACELIDFLNFNVKFMEQIY
                     EQQPLSSPGQWNRLEQRPLEGFVFAVTPFNFTSIAGNLPTAPAIMGNTVLWKPASTAV
                     LSAYYFMKLLQEAGLPDGVINFIPGTGRNVGPNVLSNSLLAGVHFTGSTAVFQSMWKT
                     IGENIANYRGYPRIVGETGGKDFIFAHVSADLDELNTAIIRGAFEYQGQKCSAASRVY
                     IPKSFWPPLKEKMLASLSTIKIGDVRDFRNFINAVIDKTAFDNILSYINYAKNSRDAE
                     IIAGGKGDATNGYFVEPTVIITTDPHFKTLEEEIFGPVMTVFVYDDNKYEETLNLCNM
                     TSPYALTGAIFARDRQAILLADKILLHAAGNFYINDKPTGAVVGQQPFGGGRASGTND
                     KAGSMINLLRWVSPRATKELFVPPKDSHYPYMIEE"
     gene            complement(1955..>2665)
                     /locus_tag="PLI55_09320"
     CDS             complement(1955..>2665)
                     /locus_tag="PLI55_09320"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPB01068.1"
                     /translation="EKALRAIEILHRLDESSQPDQEFVKYQGAYIRKSVLKGAIQNYE
                     LIPKIFYGNLIMQKIGSIDNLTWDKVKNIFTGEADKSAENWEDLSGMFATSAEISKIN
                     LALRENRLNSIVDLECALRDVHQNYEENTWQFCLNLLFKRFATNALTSEQVCQLLDEW
                     QASVVRFNNLVLKDAEKEFAQCSRVGYGIDGDATIRDQDFEAVRGTFADNKFVVELKK
                     ESEEAVKKVERICAALRK"
     assembly_gap    2666..2765
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2766..3526)
                     /locus_tag="PLI55_09325"
     CDS             complement(<2766..3526)
                     /locus_tag="PLI55_09325"
                     /inference="COORDINATES: protein motif:HMM:NF027638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HPB01069.1"
                     /translation="MENRQLTQAEINLLEKQGCRAENWEKIIIKPDFNPANVHNVTFA
                     GAVEIGNCCGVQEVKPGCLKNCGIYDSYIRNCTIADNVYIANVKNLINYTIEKDVIIE
                     NVGDLIVTGETTFGNGTELEILNEGGGRILPIYDRLTAQIAYMIVVYKHDPALQKALK
                     KLVSDYVAKRKANRGVISQGVRIYNSNIIRNVQIGEKATVSGALLLEDGTIAGSEVDP
                     VYIGEGVIAKHFIIQSGVRVDSSAVLDKCFVGQGTR"
     gene            complement(3542..4348)
                     /gene="nagB"
                     /locus_tag="PLI55_09330"
     CDS             complement(3542..4348)
                     /gene="nagB"
                     /locus_tag="PLI55_09330"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HPB01070.1"
                     /translation="MRVIIHNNYEKLSDWVARYVAAKIIHANQTSAKPFVLGLPTGSS
                     PIGTYQALVRLYKEGKVSFKNVITFNMDEYVGLSEDDPESYHYFMRHYLFDHIDIPPE
                     NINILDGNAKDLEKECEEYEAKIKRVGGIDFFLGGIGVNGHIAFNEPGSSISSRTRIK
                     TLSYDTIVANSRFFGGDLSRVPKTALTVGVGTVLDAREVMVIASGYTKARALQKVIEE
                     GVNHMWTVSMLQLHPHGTIVCDDEATLELKVGTVEYFKDIEDEAINNLPV"
BASE COUNT         1262 a         1009 c          801 g         1311 t
ORIGIN      
        1 caactttaaa tattcaacct cataattttc gcaaaaatca ctatgaaaaa tatgtattat
       61 aaagccaaac aatggcgatt aatactgtat tcgtcgtcat aaacaacttc atatttccaa
      121 aattccacta aaatataaat ttttactact taacaacgtt taatcctacc aattcagaaa
      181 atagtttatt aatttaagtc agtgttaagc attttaacat tgtatcccaa gttcaacgcc
      241 agagaggaga gttatcaatg agtaacgcca agttcacctt accccaaccc gttaatgaac
      301 cgcaattttc ctatttgccc ggcagttcgg agcgccaggc tttaaaaatt gctttaaata
      361 aaatagccgg cgaacagatt gaaatcccgt taattatcgg cggcaaggaa atccgcaccg
      421 gacaaaccgc gaaatgcatt atgcctcaca atcatcagca tattctggct atctatcatc
      481 aggcaactgc caaagaagtt agcctggcag tcgaggcttc tcaaaaagcc tgggaatttt
      541 ggtcagttat gccctggcac gaacgcgccg caattttcaa aaaagccgcg gcattgctat
      601 ccggtccttg gcgtatgacg ctcaatgcgg cgacaatgct caatcaaagt aaaaatgttt
      661 tccaggcaga aattgattca gcgtgcgagt taatcgattt cctcaatttt aatgtcaaat
      721 ttatggaaca aatttatgag caacagccgt tatcatcacc tggtcaatgg aaccgattgg
      781 aacagcgtcc cctggaaggt tttgtttttg ccgtaacgcc gttcaatttt acttccatcg
      841 ccggtaattt gccgactgct ccagctataa tgggcaatac cgttctctgg aaaccagcca
      901 gcaccgcggt gctctccgct tattatttta tgaaattatt acaagaagcc ggactgccag
      961 acggtgtaat taactttatc cctggaaccg gtcgcaatgt aggaccgaat gttctttcta
     1021 attcgctact ggcaggtgtg catttcaccg gctcgacggc cgtttttcaa agtatgtgga
     1081 aaactattgg tgaaaatatt gccaattacc gtggttatcc acgcatcgtc ggggaaaccg
     1141 gcggtaagga tttcatcttt gctcacgtca gtgccgatct ggatgaactt aataccgcga
     1201 ttattcgcgg cgcttttgaa tatcaaggcc aaaaatgttc ggcggcttct cgggtttaca
     1261 ttccaaaatc gttctggcca ccattaaagg aaaaaatgct cgcttctctt agcacgatta
     1321 aaatcggcga cgtgcgcgat ttccgcaatt ttatcaatgc tgtaattgac aaaaccgctt
     1381 tcgataatat tttatcgtat atcaattacg ctaaaaattc ccgcgatgcg gaaataattg
     1441 ctggtggaaa aggcgacgct actaacggct atttcgtcga gccaactgtt ataataacga
     1501 ccgatccgca tttcaaaact cttgaagaag aaatattcgg cccggttatg accgtttttg
     1561 tctatgacga taataaatac gaagaaactc tcaatctctg taatatgact tcgccgtacg
     1621 ccctgaccgg cgctattttc gctcgtgatc ggcaggctat cctgctcgcg gacaaaattc
     1681 tactccacgc cgccggtaat ttctatatca atgataaacc aaccggtgca gtcgtcggtc
     1741 aacaaccatt cggtggcgga cgtgcttccg gtacgaatga taaagccggt agtatgatta
     1801 accttttgcg ttgggtctcg ccacgcgcga cgaaggaact tttcgttccg ccaaaagatt
     1861 ctcactatcc gtatatgatc gaagaataaa ttaataagcc cgtgaattaa ttcatgggca
     1921 tgttattagt gagtttaatg tttttgaggc ttttttattt tcggagagcc gcgcaaatac
     1981 gctcgacttt ttttacggct tcttccgatt cctttttaag ttccaccacg aatttattat
     2041 ctgcaaaagt ccctcgcacg gcttcaaagt cctgatcacg gatagtcgcg tcaccatcaa
     2101 ttccataccc gactcgactg cattgagcaa attccttttc agcatctttg agaaccagat
     2161 tattaaagcg aacgacactt gcctgccatt catctagcaa ttggcaaacc tgttcggaag
     2221 tcaaggcgtt agtcgcaaaa cgtttaaaaa gcaaattgag acagaattgc caggtatttt
     2281 cttcataatt ttggtggaca tctcttaaag cacattccaa atcaacaatt gaattaaggc
     2341 gattctcccg cagtgctaaa ttgatcttac ttatttccgc gctggtagca aacataccgc
     2401 taagatcttc ccaattttct gccgatttat ccgcttcacc agtaaaaata tttttcactt
     2461 tatcccaggt taaattatcg atcgaaccaa ttttctgcat aatcaaattg ccatagaaga
     2521 ttttcggaat caactcataa ttttggatag ccccttttaa gactgacttt cgaatatacg
     2581 caccttgata tttaacgaat tcttgatccg gctgtgaaga ttcatcaaga cgatggagta
     2641 tttcaatagc gcgaagagcc ttttcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnncgcgt tccctgacca acaaaacatt tgtcaagcac tgcagaactg tccactcgca
     2821 cgccgctttg tattataaaa tgtttagcga ttacaccttc tccaatgtaa accggatcca
     2881 cctcagaacc ggcaatggtt ccgtcttcga gtagtaatgc tcctgaaacg gtagcttttt
     2941 cccctatttg cacgttgcgg atgatattac tgttgtaaat tctgactcct tgagaaatta
     3001 caccacggtt cgctttgcgt ttagcgacat aatcgcttac caactttttc agtgctttct
     3061 gcaaagccgg atcgtgcttg taaacgacaa tcatataggc aatttgagcc gtcaaacgat
     3121 cataaatggg taaaatgcgc ccgccaccct cattgagaat ttccagctcg gtaccgttgc
     3181 cgaaagtcgt ttcgccggta acaatcagat caccaacgtt ttcaattatg acatccttct
     3241 cgattgtata attgattaaa ttttttacgt tagcgatata gacattatcg gcaatcgtac
     3301 aattgcggat atagctatca taaatgccac aatttttcag acagcccggt ttgacttcct
     3361 gaacaccaca gcaattacct atttctactg ctccggcgaa tgtaacatta tggacattag
     3421 caggattaaa atcgggctta attatgattt tttcccagtt ttctgcccga cagccttgtt
     3481 tttcgagaag gttaatttct gcttgagtta attggcgatt ttccattgcc taattttcct
     3541 actaaaccgg caggttattg atagcttcat cctcgatatc tttgaaatat tctacggtac
     3601 cgactttcaa ttcgagcgtg gcttcatcat cacaaacaat agtgccgtgc ggatgcaact
     3661 gtaacatcga aaccgtccac atatggttga caccttcttc gatgactttc tgcaaggctc
     3721 gcgctttggt gtatccagag gctatcacca ttacttctcg ggcgtccagc accgttccga
     3781 cacccacggt taaagccgtc ttaggtactc ggctaaggtc accaccgaaa aagcgcgaat
     3841 tagcaacaat cgtatcatag gacagcgtct ttattcgagt ccgtgaagaa attgaagaac
     3901 ccggttcgtt gaaagcaata tgtccattca ccccgattcc tcccagaaaa aaatcaatgc
     3961 cgccgactct tttgattttg gcttcgtatt cttcacattc tttttcaaga tctttggcat
     4021 ttccatccag tatattaata ttttcaggcg gaatatcaat gtgatcaaat aaataatgcc
     4081 gcataaaata atgataactt tccgggtcat cttccgaaag tcctacatac tcatccatat
     4141 taaaagtaat gacattttta aatgatactt taccttcttt atacaatctt accagcgcct
     4201 ggtaagtacc gattggcgaa gagccagttg gtaaaccgag cacaaaaggt ttagcgctag
     4261 tctgattggc atgaataatt ttagccgcaa catatcgggc tacccaatca ctcagtttct
     4321 cataattatt gtgaattatt accctcattt taaattcctt tactaatgaa taattttact
     4381 tttactgaat tactgaatca aaaataccat ttaaagttct tgtagttaca attttatata
     4441 aaattaccaa tcacaaaaat aatatataac aaaaatataa tta
//
LOCUS       DAOOMU010000128         4413 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_23858_length_4413_cov_7.449518, whole genome shotgun sequence.
ACCESSION   DAOOMU010000128 DAOOMU010000000
VERSION     DAOOMU010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23858_length_4413_cov_7.449518"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLI55_09335"
     CDS             <1..215
                     /locus_tag="PLI55_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HPB01071.1"
                     /translation="RQPALFRKVRKRIETLFSQLVDQFVIRVNYAKTFVGLATRILSK
                     VTAFILLQFLNSINNKPLNHIKHALA"
     gene            262..954
                     /locus_tag="PLI55_09340"
     CDS             262..954
                     /locus_tag="PLI55_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01072.1"
                     /translation="MKRIILIVLIGLINTTIFTQVKYPSRDSKEWNDLTVAQRWQAVN
                     IPEDQLSQMSTNDLIEHCIDFDFMWDIFNHSNYGIGLNVVIENHNGLRELLNRSDAGE
                     LLLDFYNKIDLNKIKEITESADRGEFVGQIFFLELFLSYPSVLKQFQGNEKILIDSIL
                     KTYDTCLEMNEKSEKDYYCGYSVNTKILAMGRALDQLKGRETTAAALEEMDLSQLTEE
                     QCQEILNEAREL"
     gene            954..1883
                     /locus_tag="PLI55_09345"
     CDS             954..1883
                     /locus_tag="PLI55_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HPB01073.1"
                     /translation="MRKMLRDILFIGMLFHFLSYQFSCDDSVEPSEYDSWIPELVLID
                     EDLTFTMGCVDSLGFIGVHRDVYPYFTAILDSYYIGKYEVRNDEYNYFVADSGYYDAS
                     LWSETGWEYIQSERRTRPVDWVEGNEPWINCELSNTPDRPINNITWYEAEAYCNWLSR
                     ETGETYALPTEAQWERAARGPDPGRPFAYGRVHDADKYNNMMYSGELYPVGYYKKDKS
                     YDGCYDMAGNLLEFCSDLYDVEIYQEYKENEPVYNPKGPDSSLIGSRSVRGIFNFYHQ
                     DWDIEYQIQTITRISIDPNAYVQSDGFRVARIK"
     gene            1900..2595
                     /locus_tag="PLI55_09350"
     CDS             1900..2595
                     /locus_tag="PLI55_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01074.1"
                     /translation="MKKLIIMLILLLVAINIYAATLYTPNGKTFTTTDANRLSYDADW
                     FERHKENYGPGTLYPNSEVMGEWEDGLWEYNCHVFAWNNWQAADRWSSESDMWKLGKP
                     SPYNLMWRSYPDVWYTDTDNPIGIVSYVSASSSEAAICTYSGNHSARIVGNGTYFISK
                     WGSNPIIKHPPTEVPTSYYSVNAYYKINPAYRPIGEGDPAGRDWETISNAISGQGTGK
                     TFSNGLWSGLGDN"
     gene            2525..3283
                     /locus_tag="PLI55_09355"
     CDS             2525..3283
                     /locus_tag="PLI55_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01075.1"
                     /translation="MRLVVKVPEKLSRTGFGRDWETISNAISGATSGSLISVLSGTHT
                     LTGNVTVPSGVTLTIASGAMVNYNGYTLSSSGGTISIQSGGIITLNPATYVDFSGSGN
                     TYEVNGINVGASFNGIGQSIKAIPPSGYGIAGWSDGVGGNYREINGNVTVNARLKALH
                     KSNTIAAWDNTSQRKLIQTSAGGNPRWLHQVYTSAGHVWLEHSGDGGSTWTLGNNGQP
                     LDGTAGGKCPSIAFTTHYTGSTTDNYIGVVWQEK"
     gene            3371..3658
                     /locus_tag="PLI55_09360"
     CDS             3371..3658
                     /locus_tag="PLI55_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01076.1"
                     /translation="MKPEERKTVGFFISNSEGKDCRKGMDLSRDSDFQTNNIPVNRNK
                     SNNGNFRSSGRIPFKVYKPENYAGLLSLTISLFFKYQLFCQTGGAKFIFRV"
     gene            3840..>4413
                     /locus_tag="PLI55_09365"
     CDS             3840..>4413
                     /locus_tag="PLI55_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01077.1"
                     /translation="MTSDKKFKLLLNTMIVTVLSLLLFLSSCPEELDYYDDSDTTSHD
                     FLWETYTFGDLASNDLNDVVVIDENNIWAVGEIFVYDPDSSWNGTGIETFNAVHWNGT
                     EWEYIHIQGSLMNDAGPLYSIWYFDECNIWVTTFGLPIHWDGNEWTLYHIQNMGIDAS
                     AGYGIWASSPSNIYFVGYKGSIVHYDGKNFR"
BASE COUNT         1352 a          812 c          992 g         1257 t
ORIGIN      
        1 gaagacaacc ggcattgttt cgaaaagttc gtaaacggat tgaaaccttg ttttcgcagt
       61 tggtagatca attcgtgatt cgtgtaaatt atgccaaaac ctttgtcggc ctagctacca
      121 ggattttgtc gaaagtgact gccttcattt tgctccagtt cctgaattct atcaataata
      181 aacccctaaa ccatattaaa catgccctcg cttaattcca cacaacgggt tatatctatg
      241 tataaatatt atggaggtaa aatgaaaagg attattttaa tagttttaat tggtttaata
      301 aacaccacca ttttcacgca agtaaagtat ccaagccggg attcaaagga atggaatgat
      361 cttacagtag ctcagagatg gcaagctgtg aacatacctg aagatcagct cagtcaaatg
      421 tctacgaatg acctaattga gcattgtatc gatttcgatt tcatgtggga cattttcaat
      481 cattcgaatt atggtattgg attaaatgtg gttattgaga atcataatgg attgcgagaa
      541 ttgttgaatc ggagtgatgc tggagaattg ctcttggatt tttataataa aatcgatctg
      601 aacaagatta aagaaataac agaatcagca gaccgtggag aattcgttgg ccagattttt
      661 tttcttgaac tctttctaag ttatccaagc gtattgaagc aatttcaggg aaatgaaaag
      721 atattgatcg actctatttt gaagacatat gatacttgtc ttgaaatgaa tgagaaatca
      781 gaaaaagatt actattgcgg ctattccgtt aatacaaaaa ttctggcaat gggaagagca
      841 ttggatcaat taaaaggcag agaaacgaca gctgcggcat tagaagaaat ggatttgagt
      901 cagttgacgg aagaacaatg tcaggaaata ctcaatgaag cacgggaatt gtgatgagaa
      961 aaatgttaag agatatcctg ttcataggga tgctatttca ttttctatcc tatcaatttt
     1021 catgcgatga ttctgtagag ccgtctgaat acgatagctg gataccggag cttgtcttga
     1081 tcgacgagga tttaaccttt accatgggat gtgtggattc tctgggtttc attggcgtgc
     1141 atcgtgatgt ctatccctat tttacggcga ttttagattc ctactatatc ggcaagtacg
     1201 aagtgcggaa tgatgaatac aattattttg tggccgatag cggttattac gacgcgtctc
     1261 tctggtctga aacaggctgg gaatatatcc aatcggaaag acggacgcgt ccggttgatt
     1321 gggttgaagg caatgaacct tggataaatt gcgaactttc gaatacaccc gacagaccta
     1381 ttaacaatat cacttggtat gaagcggaag cctattgtaa ctggctcagc agggaaacgg
     1441 gagagaccta tgccttgcca acggaagcgc aatgggaaag agcggcgcgg ggtcccgatc
     1501 cggggcggcc atttgcctat ggcagggttc acgacgcgga caaatacaac aatatgatgt
     1561 acagcggtga attgtatccg gttggatatt ataagaaaga taaatcatac gatggctgtt
     1621 atgacatggc cggtaatctt ttggaattct gttctgactt atacgacgtt gaaatctatc
     1681 aagagtataa agaaaacgaa ccagtatata atccaaaagg gccggatagt agtctaatag
     1741 gtagtagatc tgtgcgagga atttttaatt tttatcacca agactgggat atcgaatatc
     1801 aaatacaaac tattacaaga ataagcatag atccaaatgc atatgttcaa tctgatggtt
     1861 ttagagtggc aagaattaaa taagagagga aaataaatta tgaagaaatt aatcataatg
     1921 ttgatattac tccttgtagc aatcaatatt tatgctgcaa cgttatacac acccaatggc
     1981 aaaacattta caactacgga tgcgaaccgt ttatcttatg acgctgattg gttcgagagg
     2041 cataaagaaa actatggacc tggaactcta tatccaaatt cagaagttat gggcgaatgg
     2101 gaagatggac tctgggaata caattgtcat gtttttgcct ggaacaactg gcaggccgca
     2161 gatagatgga gtagcgagag cgatatgtgg aaactgggaa aaccttcacc ttataatttg
     2221 atgtggcgat cctatccgga tgtctggtat actgataccg ataatccgat tggcattgtg
     2281 tcctatgttt ctgcatcttc aagcgaagcg gcaatctgta cttatagcgg gaatcattcc
     2341 gcaaggatcg ttggtaatgg aacatatttc atctccaaat gggggagcaa tccaattatc
     2401 aaacatccac caacagaagt acctacaagc tattactctg taaacgcata ttacaaaatc
     2461 aatcctgcct accgaccgat tggcgagggc gatcctgccg gtcgggattg ggagacaatt
     2521 agcaatgcga ttagtggtca aggtaccgga aaaactttct cgaacgggct ttggtcggga
     2581 ttgggagaca attagcaatg cgattagtgg tgcaaccagt ggaagtttga tttcagtatt
     2641 gtccggcaca catactttaa ctggaaatgt tacggttcct tccggtgtta ccttaacaat
     2701 tgcttcaggt gcaatggtca actataatgg ctatactctg agttcatcgg gtgggacaat
     2761 atccattcaa agtggcggaa tcataacact caatcccgca acgtatgttg atttttcagg
     2821 tagtggcaat acttatgaag taaatggaat caatgttggg gcatcgttta acggcattgg
     2881 acaatcaatc aaagctattc cgccgtctgg ctatggtatt gccggttgga gtgatggtgt
     2941 tggaggtaat tacagagaga ttaatggaaa tgttaccgtt aatgcaagac taaaagccct
     3001 tcacaaatcc aataccattg ccgcttggga taataccagc cagcggaaat taatccagac
     3061 ctcagccggt ggaaatcctc gctggcttca tcaggtttat accagtgccg gccatgtgtg
     3121 gcttgaacac tccggtgatg gcggcagtac ctggacgctt ggcaataatg gacagccctt
     3181 agatggtacc gccggtggca agtgcccttc gattgccttc actacccatt atacaggttc
     3241 aacgacagat aattatatcg gtgtagtatg gcaggagaaa taatcttaca aaaaaacttt
     3301 ttaaaaaaac tcttgacttt accccccccc cccatcatat agattatacc agaagcaaga
     3361 agagtcttcg atgaaaccag aagagcggaa aaccgtagga ttttttattt ctaactcaga
     3421 agggaaggat tgtaggaaag gaatggattt gtctcgagat tcagatttcc aaacaaacaa
     3481 tattccggtc aatcggaaca aatcaaataa tggcaacttt aggagcagtg gtaggattcc
     3541 ttttaaggtt tataagcctg aaaattatgc gggcttactg tcccttacca tttctctttt
     3601 tttcaaatat caacttttct gccagacagg aggggctaaa tttattttcc gtgtttagaa
     3661 atatgtgagc taattttagg agttaaaaat gcaactcaaa aaaccgctca atacccgcgc
     3721 ttgactatga tagcagtccg ggcgcgggag ctattcgcca gaggcggaaa cacaacggtt
     3781 gaaattatga tgaaatgagt gtactttcaa ttgtgaaaag aaaaaaataa ggaaaaacga
     3841 tgacctctga caagaaattc aaactactcc tgaatactat gattgtgact gttctatctc
     3901 tacttttatt cctctccagc tgtcccgaag agctggatta ctatgatgat tccgatacta
     3961 ccagccatga ttttctatgg gaaacctata cttttggtga tcttgccagc aatgatctca
     4021 atgatgttgt cgtaatagat gagaacaaca tctgggctgt aggtgagatt tttgtttacg
     4081 atccggattc ctcctggaat ggcaccggca tagagacctt taatgccgtc cattggaatg
     4141 gaaccgaatg ggaatatatc cacatacaag gtagtcttat gaatgatgcc ggcccacttt
     4201 atagtatctg gtatttcgat gaatgcaata tttgggtcac tactttcgga ttacctattc
     4261 attgggatgg aaacgaatgg actttatatc atattcaaaa tatgggtatt gatgccagcg
     4321 ccggttatgg tatctgggcg tcttctcctt ctaacattta tttcgtaggg tataagggtt
     4381 cgattgtcca ctacgatggc aagaatttca gga
//
LOCUS       DAOOMU010000129         4363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_24196_length_4363_cov_16.455200, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000129 DAOOMU010000000
VERSION     DAOOMU010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24196_length_4363_cov_16.455200"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   19..276
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=93..130
                     /rpt_unit_seq="gtctaagtcctttatccactaaaccaaggattgaaacc"
     gene            604..2568
                     /locus_tag="PLI55_09370"
     CDS             604..2568
                     /locus_tag="PLI55_09370"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPB01078.1"
                     /translation="MKRFLLTIIFLISITAFSLAQISSDWIEQEIRTKTVWLEIQRAA
                     LLQLPSYANTNAYDVTYYTLDFRFDIASSILYGEVTIRGLAVESNLQQIDLDMDDNLS
                     VTSISGDAFSYTHRNNILSLQLNKILKENEIFNVSITYSCLEKKNNDRGIYFQYHDSA
                     PVISTLSEPYYAHTWFPCKDRLGDKADSVDINITVPSELKAVSNGTLVRIIENDDGTC
                     TYCWQERYPIAVYLISLAISDYTYWSENYFGIDGFSSMPLEFWIYPDFDTIARPFLNM
                     TADMMTHFASLWGEYPFIKEKYGQVQFSWGGGMEHQTATSLGSFSEMLICHELAHSWW
                     GNDVTCASWKHIWLNEGFARYAEALWREKDEGTIGLKNYMTDLNRPSRWRAGSLYVQD
                     TSDVNNIFNQIVYDKGAWVLHMLRGLTGYEKFFEILKRYRQEYSGGFATTADFQKKCE
                     EIYDQSLDWFFEQWVYGVGQPHYQVNWQAYRHSLNSWKLKVAISQIQTGATIFRMPLT
                     LLFSDSLNDTTFIVTDSLATQYFTFYCNFKPDSVALDPDNWVLKNATYFNSYSEPQTR
                     PEEFRLHPPYPNPFNQWVNIKLDLPYDIPGRLAIYDLTGREVAILQDGLFKAGQYKTQ
                     WQPQGSASGLYFVSFKARYIHLRQKIVYMK"
     gene            2642..3175
                     /locus_tag="PLI55_09375"
     CDS             2642..3175
                     /locus_tag="PLI55_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01079.1"
                     /translation="MLTKIKNALRQPENRRIIAIVILLFIIINVIAWLSLRPYMKQPP
                     CYVCGRAKTRPVKTLYQYRVEVLPYMKSVDIWYCERHYQHAAEIITKLPIKNDSVASR
                     FRIAVIAGTFSLFSLLFIIILLEISFNWLFIHSGLIALTYLIGGVTSNLTVVILLIST
                     FALAIGIFYFWNRWFNK"
     gene            3185..4297
                     /locus_tag="PLI55_09380"
     CDS             3185..4297
                     /locus_tag="PLI55_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPB01080.1"
                     /translation="MDKVDIVIIGAGVVGLAIARELSAKLQAEIVLLEKHPYFGAETS
                     SRNSEVIHGGMYYPTDSLKARFCVRGNRQLYEICQKYRIPYRKTGKLIVASTAEELAK
                     VEALHQQGNLNGVPNLVMLSEKQIREIEPEVRAKNALYSPETGLIDVHRLMQFYESQA
                     LSNGVLIAYQNTVTGIEKQSDEYRITVSDETGADWDILSQVVINSAGLNADKIAALVG
                     INIDENHYRLHYCKGEYFNVSNRHQGKIQHLIYPVPTDYSLGIHARLRLDGTLSLGPN
                     AIYVDEINYEVDPGHRADFYNSVKDILPYIKYEDLTPEMAGIRAKLQGHGERFRDFVI
                     REESDKGLPGFINLIGIDSPGLTSAPAIAEYVASLL"
BASE COUNT         1281 a          916 c          921 g         1245 t
ORIGIN      
        1 ttttctgtcg gttgccgtgt ctaagtcctt tatccactaa accaaggatt gaaactttca
       61 acaccagaga aattggcggt gagtttcaac gggtctaagt cctttatcca ctaaaccaag
      121 gattgaaacc gcactgctat tcaagtcaat atggactaga gaaatgtcta agtcctttat
      181 ccactaaacc aaggattgaa acccaagcct accatagcga tgataggcga agctggtggt
      241 ctaagtcctt tatccactaa accaaggatt gaaaccaccg gcgaggaaag tagatatttt
      301 ggttattcat gaatggcaaa gagtgaataa atatgtggtt aattggcaac acaaaagtct
      361 cgctatggac tatacattta ccgattaacc attaaatctg caaattttaa aagaatattg
      421 aacaagaaaa tggtagttat agagtaaagg aatatggagt tcctatggag tgcatatatg
      481 gagtgcagtg accatgtcac tgcatgcacc gacacggtcg gtgcactcca aaattacact
      541 ccaaaactgc aaaattgaaa ccgctggaaa acttatcagc aatagttaac ttttcactgc
      601 cttatgaaaa gatttttatt aactattatt ttccttataa gcatcactgc cttctctcta
      661 gctcaaatta gctccgattg gatcgagcaa gaaatccgta ctaaaaccgt atggctggaa
      721 atccagcgag ccgcactgct ccaactaccg agttacgcaa atacaaacgc ttacgacgta
      781 acttattaca ctctggattt tcggttcgac attgccagct caattttata cggtgaggtt
      841 accatccgcg gattagccgt tgaatctaac cttcaacaaa ttgacctcga tatggatgac
      901 aacctatcgg taacatcaat tagcggagat gccttcagct atactcatcg caacaatatt
      961 ttatcattgc aactgaataa aattctgaaa gagaacgaga tcttcaacgt ctcgattacc
     1021 tatagttgct tagagaaaaa gaataatgac agagggatat attttcaata tcacgattca
     1081 gcgccggtca tctcaactct gagcgaaccc tattatgctc atacttggtt tccttgcaaa
     1141 gatcggctcg gtgataaagc cgattccgtg gacattaata ttaccgtacc gtcggaattg
     1201 aaagccgttt caaacggcac tctggtcaga ataattgaaa atgatgatgg cacctgcact
     1261 tattgctggc aggaacgcta cccgattgcc gtttatctaa tttcattagc aatttccgac
     1321 tacacttatt ggagtgaaaa ttatttcggt atagacgggt tcagctcgat gccgctggaa
     1381 ttctggattt atccagattt tgacacaatt gctcgtccgt ttctgaatat gactgccgat
     1441 atgatgactc actttgcttc cctctggggt gaatatccgt tcatcaaaga aaaatacggt
     1501 caagttcaat tttcctgggg cggtggtatg gagcaccaaa ccgctaccag tctgggatcg
     1561 ttttctgaga tgctgatttg tcacgaatta gcgcattcct ggtgggggaa tgatgttact
     1621 tgcgccagct ggaagcacat ctggcttaat gaaggctttg cccgctatgc agaggcactt
     1681 tggcgggaaa aagatgaagg taccattgga ttaaagaatt acatgacgga tctgaatcgc
     1741 ccttctcgct ggcgcgccgg tagtttgtat gttcaggata ccagtgacgt taacaatatc
     1801 ttcaatcaga tagtatacga taaaggcgcc tgggtattgc atatgctaag aggcttgacc
     1861 ggatatgaaa aatttttcga aattcttaag cgataccgcc aggaatactc cggcggtttt
     1921 gccaccactg ccgatttcca gaaaaagtgt gaagaaattt acgaccaaag tttagattgg
     1981 ttcttcgagc aatgggttta cggcgtcggt cagcctcatt accaggttaa ttggcaagcc
     2041 tatcgccatt ctcttaattc ctggaaatta aaggttgcta tctcacaaat ccagaccggt
     2101 gcaacaattt tccgaatgcc gctgactttg ttgtttagcg acagcctcaa tgatacaact
     2161 tttatagtca ccgacagttt ggcaacccaa tatttcactt tttactgcaa ttttaaaccg
     2221 gattccgtcg ccctcgatcc agacaattgg gtattaaaga atgcgactta tttcaattcc
     2281 tactcggagc cgcagactcg accggaggag ttccggcttc atccgcccta ccccaatcct
     2341 tttaaccaat gggtaaatat aaaattagat ttgccgtatg acatccccgg gcggctagca
     2401 atttacgatt taaccggccg ggaagtcgcc attctacaag atggtttatt caaagccggt
     2461 caatataaaa cccaatggca accgcaaggt agcgcctccg gtctttattt cgtcagtttc
     2521 aaagcgaggt acattcatct ccggcaaaaa attgtatata tgaagtaaga tttatcttaa
     2581 ccgccagaat taatttttat aaattcagcc caaaatataa acaacgagaa ttttgagata
     2641 aatgctgact aaaattaaaa atgctttgcg ccaaccggag aaccggcgaa ttattgccat
     2701 tgttatcttg ctatttatta tcataaatgt gatagcctgg ctatcgcttc gcccgtatat
     2761 gaaacagccg ccttgttatg tttgcggacg ggcaaagacc cgtccggtca aaacgcttta
     2821 tcaataccga gttgaggttt tgccctatat gaaatccgtt gacatttggt actgcgaacg
     2881 gcattatcaa catgcggctg agattatcac caagttaccg attaagaatg attcggtggc
     2941 tagtcgtttt agaatcgccg ttatcgccgg aactttttca cttttttcgc tactatttat
     3001 aatcatttta ctggaaatta gttttaactg gctttttatc cattctggat taattgcgct
     3061 tacttacttg ataggaggcg tgacttccaa cttaactgtg gtaatattat taatttcaac
     3121 ctttgcactc gcaatcggaa ttttttattt ttggaatcgt tggttcaata aataatagac
     3181 taaaatggac aaagtggata tcgtaattat cggagctggt gtggttggat tagcaatcgc
     3241 acgtgaactt tccgcaaaac ttcaggcaga aattgtgttg ctggaaaaac acccttattt
     3301 cggagcagaa acctcttcgc gtaacagcga ggtcattcat ggcggaatgt attatccgac
     3361 ggacagtctc aaagcccggt tttgcgtgcg cggtaatcgt caactctacg aaatttgtca
     3421 aaaataccga atcccctatc gcaaaaccgg caaactgatt gttgcttcta ccgctgaaga
     3481 attggcaaaa gtcgaggcac tccaccagca aggcaatctg aatggtgtcc ccaatctagt
     3541 gatgttgagc gaaaaacaga ttcgggaaat agaaccggaa gtaagggcga aaaatgctct
     3601 atacagtcca gaaaccggct taattgatgt tcaccgtcta atgcagtttt atgagtcgca
     3661 agccctttca aacggcgtct tgatcgccta ccaaaatact gtaaccggga tagagaaaca
     3721 gtccgatgag tacaggatta cagtatccga tgaaacaggt gcagactggg atattttaag
     3781 tcaagttgtg attaattcgg ctgggctgaa tgcggacaaa attgcggctt tagttggtat
     3841 caatattgac gagaatcatt atcgtttgca ttattgcaag ggtgaatatt tcaatgtttc
     3901 taaccggcat caggggaaaa tccagcacct aatttatccg gtaccgactg attatagcct
     3961 gggcattcat gctcgcctac gattagacgg gacgctgtca ctcggaccaa atgctattta
     4021 cgttgatgaa attaattacg aggtagatcc cgggcataga gcagattttt ataacagcgt
     4081 gaaagatatt ttaccttata ttaaatacga agaccttacg cccgaaatgg ctgggattcg
     4141 agcaaagttg cagggacatg gtgaaaggtt tcgcgatttt gtgattcgtg aggaaagtga
     4201 taaaggattg ccgggtttta tcaatttaat tggcattgat tcaccgggat tgacttcggc
     4261 accagccatc gccgaatatg tagccagctt gctttaaatt aataagccat tttttcaatt
     4321 aaaacttgta ggacagcctt tcaaggctgt cgaaaataga caa
//
LOCUS       DAOOMU010000130         4268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_24916_length_4268_cov_4.239259, whole genome shotgun sequence.
ACCESSION   DAOOMU010000130 DAOOMU010000000
VERSION     DAOOMU010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24916_length_4268_cov_4.239259"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="PLI55_09385"
     CDS             <1..1373
                     /locus_tag="PLI55_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB01081.1"
                     /translation="CTVWNIGKEGIFDNACGTSDYIELKNSTFHHINREILRLKTQNP
                     KVIIDHVTVDSCGFGYGVEGAKFAAFKVEVPNEISITNSIVSNVPNSVYGYSVRFYGE
                     KSYMNNVLMYNTPKIDDNNGSVLGRDIYWYDPMFAYATCYDYTLKDSSVAYHLAGDGS
                     VSIGDLKWATSTNIATYYMLDIVTGGHGKVSVTPAPMAKFYIPSTMVTLTAIPDTLYK
                     MGEWSGDATGTESLIQITMNSNKTIQVSFLKAYYLVTMNVNMSYWTYLGKFSIENDSV
                     DVAGNFNNWGEKSIWMSDENADSIYTADIKIDENYPDIQWKFRINGSWDNATCEFPFG
                     GPNRSYTVICDTVLTYWYNDQQFPSDGLLSELIPDHYELNQNYPNPFNPVTTIKFALK
                     QDGFTTLTVYDMLGREVARLVNQEMRAGYHQVVFSDIGKLASGVYIYKLTSGNFNSVK
                     KMMLMK"
     gene            1443..4229
                     /locus_tag="PLI55_09390"
     CDS             1443..4229
                     /locus_tag="PLI55_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HPB01082.1"
                     /translation="MNCKIKGILNSVISSIEIRNFQKYFTLSLVLLITQMSLLEAGEY
                     YVKSASEIYSLISTIQPGDTITMSNGTWKDQHIVFHANGTQEKPILIRAETPGYVRLQ
                     GNSYLKFSGSWITVNGLLFRNGYCASGQSVIEFRSSFGRANHCRLTNSVIVDYNPSDK
                     NIDYKWVSLYGRNNRVDHCHFEGKNHSGTTFVIWLDKESDRENYHQIDHNYFGPRPEL
                     GYNGGETIRIGTSDYSLTNSNSIVEYNVFEECNGETEIISSKSCENIYQYNTFINCKG
                     CLTLRHGERCIVRGNYFFGNRQDGTGGVRIIGVDHQVYNNYFDGLNGSGYSSALCIVK
                     GVQNSPLNRYYQVERALVAFNTFINCGYSLLIGYGSDSDQTLPPKDCIIANNAIQTNY
                     QVVKIGDSQGAPVNFTWEGNIFYGSSLGISNPGGITWQSPELSISSDGLYRPSMSSPL
                     IDAAVGDYPSITNDFDGQNRTSRFDVGCDELSSDSITIRPLTAEDVGVTWFVPGPLSL
                     EIVAGENTLLDAISLLGDEDTLKLITDGGLYQLTEKLSVNRQIIIKAASGLAEKPVVT
                     RSVIDSGDSVLIEMNMQGKLYLDGVILDGGANTENRISAIISSSVSPFSVYFRIKANN
                     CEFRAISNNGNGSLIRLLAGSQADSVIFTNCLFSGCDGRAIRMNEETEESGKFNVLYL
                     KMKNCTFWDIASEAVNIYGGDSNPFTIGPSVKIEHCTFDDCGFYGTPTINLAEVDNAV
                     IRNSIISNSSPDTASVIIYGWAYIEYCDIFNSGLVSLNRGAHQYNGMINVDPQYYDAS
                     RGDFRLADSSPVLTAGSDGKALGDLRWVQSSSNEISHNGRISIKKFAIFRNYPNPFNG
                     ATQIVFILEKSSQVKIEIYDLNGKLVTQFNETYTIPGQYSVNWQPERNASGIYFCRLA
                     TGNLSKMIKIMYLK"
BASE COUNT         1259 a          716 c          947 g         1346 t
ORIGIN      
        1 attgtacagt ttggaatatt ggcaaagaag gtatttttga taatgcctgt ggaacttctg
       61 attacattga gcttaagaat tctactttcc accatatcaa tcgggaaatt cttcgtttga
      121 aaacgcaaaa tccaaaagta attattgacc atgttactgt tgatagctgt ggatttggtt
      181 atggagttga gggggctaaa tttgcggctt tcaaagttga agtacctaat gaaatatcca
      241 tcaccaattc aattgtctcc aatgtgccca acagtgttta tgggtattct gttcgcttct
      301 atggagaaaa gagttacatg aataatgtcc tgatgtataa tacccccaaa attgacgata
      361 acaatggtag tgtattaggt cgggatatat actggtacga tccgatgttc gcctacgcta
      421 cgtgctatga ttatacgctg aaagacagct cggtggcgta tcatttagct ggtgatggtt
      481 cggtatctat cggtgattta aaatgggcaa ccagtacgaa catagccact tattatatgc
      541 ttgatattgt aactggtgga catggaaaag tgagtgttac tccggcaccg atggctaaat
      601 tttatatccc atctaccatg gtaactttga cagcaattcc agatacttta tataaaatgg
      661 gcgaatggtc tggagatgca actggtacag agtctctaat tcagattacg atgaattcta
      721 ataagacgat ccaggttagc tttctgaaag cttattactt ggtaacgatg aatgtgaata
      781 tgagttattg gacttatctg ggtaaatttt ctattgaaaa tgattcggta gatgttgctg
      841 gtaattttaa taactgggga gaaaaatcaa tttggatgtc tgatgaaaat gctgatagta
      901 tctataccgc tgatataaaa attgatgaaa actatccaga tatccagtgg aaattccgga
      961 taaacggttc atgggacaat gctacttgtg aatttccctt cggaggacct aatcgtagtt
     1021 atactgttat ttgtgataca gttctgacat actggtataa cgatcagcaa tttccgagtg
     1081 atggtcttct atctgaattg atcccggatc attatgagct taatcagaat tacccaaatc
     1141 cctttaatcc tgtaacgact attaaatttg ccttgaagca ggatggcttt actacactga
     1201 cagtttacga tatgttggga agagaggtag cacggttggt taaccaagaa atgagagccg
     1261 gataccacca ggtagtattt tccgatatag gaaaacttgc gtcgggagtt tacatctata
     1321 aactgacttc tggtaatttt aattcggtta agaaaatgat gctaatgaag taatccttag
     1381 tgtatggtcg ggcagattat ttatctgccc gaccattttt tgtatccaga attgcaattg
     1441 ttatgaactg taaaataaaa ggtattttga attcggtaat tagttctata gagattagaa
     1501 attttcaaaa gtatttcact ctttcactgg tacttttaat tactcaaatg tctttacttg
     1561 aggctggaga atattatgtc aaaagtgcct cagaaattta tagtctgatc agtaccattc
     1621 agcctggcga tacaattacg atgtcaaatg gcacctggaa ggatcaacat atagtttttc
     1681 acgccaatgg tactcaagaa aaaccaattt taatcagagc cgagacgccc ggctatgtcc
     1741 gattgcaagg caatagctac ttaaagttca gcggtagctg gattacagtc aatggattat
     1801 tatttcgtaa tggttattgt gcatccggcc agagtgtgat tgaatttcgc agcagttttg
     1861 gccgggctaa tcattgtcgc ttgactaatt ctgtgatcgt tgattataat ccttccgata
     1921 aaaatattga ctataaatgg gtgtccttat acggtcggaa taatcgcgtt gatcattgtc
     1981 attttgaggg taaaaatcat agtgggacga cctttgtcat ttggttagat aaggaatcag
     2041 atcgcgaaaa ttaccatcaa attgaccata attactttgg gcctcggccg gagttgggat
     2101 ataatggcgg cgagaccatt cgaataggga cttcggatta ctcgctgact aattcgaatt
     2161 ctattgtaga gtataatgtt tttgaagaat gtaatggcga gacagaaatt atttccagta
     2221 agtcttgtga aaatatttat caatataaca catttattaa ttgtaaaggt tgccttaccc
     2281 tgcgtcatgg tgagcgttgc attgtacggg gaaattattt tttcgggaat cgccaggacg
     2341 gtactggcgg tgttagaatc attggagtcg atcaccaagt ttataataat tatttcgatg
     2401 gtctgaatgg ttcagggtat tcatcggcgt tgtgcatcgt aaaaggtgtg cagaattcac
     2461 ccttgaatcg ctattatcag gtagaacgag cgctggtagc tttcaataca tttattaact
     2521 gtgggtattc attattaatt ggatacggca gtgatagtga tcagactttg ccgccgaaag
     2581 attgtatcat tgctaataat gctattcaga ctaattatca agttgtcaaa attggtgaca
     2641 gtcaaggtgc gccggttaat tttacctggg aaggtaatat tttttacggt agcagtttgg
     2701 gaataagtaa tcctggtgga attacttggc agagtcctga attatccatt tcctcggatg
     2761 gactttatag accttcgatg agcagtcctt tgattgatgc cgccgttgga gattacccat
     2821 ctattaccaa cgatttcgat ggacagaatc ggacaagccg cttcgacgtc ggttgtgatg
     2881 aattgagcag tgattcaatt acgataagac cacttacggc tgaggatgta ggggtaacct
     2941 ggtttgttcc gggaccgtta tctctcgaaa ttgtcgctgg agaaaatacc ttactggatg
     3001 caataagctt gttaggtgat gaagacactc taaagttaat taccgatggt ggtttgtatc
     3061 aattgactga gaagttaagt gtgaaccgcc aaattataat caaggcggct agcggcttag
     3121 ccgaaaagcc agtagtaaca cgcagtgtga ttgattctgg ggattcagtc cttattgaaa
     3181 tgaacatgca ggggaaattg tatttagatg gagttattct ggatggtgga gcaaatactg
     3241 agaatcgaat atcggctatt atctccagct cagtttcgcc attttcggta tattttcgaa
     3301 taaaagccaa taattgtgaa tttagagcta tcagtaataa cggcaatggc agtctgatca
     3361 ggttgttagc aggatcgcaa gctgattcgg tcattttcac aaattgttta ttcagcggtt
     3421 gcgatggaag ggccatccgg atgaacgaag aaactgaaga gagtggcaaa ttcaacgttc
     3481 tatatttaaa gatgaagaac tgtacttttt gggatattgc tagtgaagct gtgaatatct
     3541 acggaggaga ttcaaatccc tttactattg gcccatctgt aaaaattgag cattgcactt
     3601 tcgatgattg cggattctac ggaactccta ccatcaattt ggctgaggtg gataatgccg
     3661 tgattcgtaa ttcaataatt agtaattcct cccccgatac ggcttcagtc attatctatg
     3721 gatgggctta catcgaatat tgtgatattt tcaatagtgg gctagtcagt ttgaatcgag
     3781 gtgcccatca atataacggg atgattaatg ttgatccaca atattatgat gctagtcgcg
     3841 gcgactttcg tttggcagat tcgtctcctg ttctgactgc gggaagtgat ggaaaggcct
     3901 taggggattt acgctgggtt caatcctcaa gtaatgagat ttcacataac ggtagaatat
     3961 ctataaaaaa attcgcgatt tttcggaatt atcctaatcc atttaatgga gcaacgcaga
     4021 tcgttttcat cctcgaaaaa tcctcacaag taaaaataga aatttatgat ttaaacggca
     4081 aattggttac acagtttaat gaaacatata ctataccagg ccaatattcc gtgaattggc
     4141 agccggagcg aaatgcgtct gggatttatt tctgccgatt agcgacaggt aatttgtcca
     4201 agatgataaa aataatgtac ctaaaatagt atgaaaaatc tcactttatt cttaatcact
     4261 gccttatt
//
LOCUS       DAOOMU010000131         4174 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_25660_length_4174_cov_17.521971, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000131 DAOOMU010000000
VERSION     DAOOMU010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25660_length_4174_cov_17.521971"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLI55_09395"
     CDS             <1..238
                     /locus_tag="PLI55_09395"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543917.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPB01083.1"
                     /translation="GFHAPTVYFPLIVKEALMIEPTETESKETLDRFIEALVKIDQEA
                     TNEPEKLRNAPWTTPVRRLDELKANKELNICWGK"
     gene            223..624
                     /locus_tag="PLI55_09400"
     CDS             223..624
                     /locus_tag="PLI55_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPB01084.1"
                     /translation="MLGEVERQIVNTTDAPRAIGPYSQAVISGNFVFTAGQLGLDPVA
                     GKIVSNDFEQQVRQIMRNLKAILQAAESDLKNVVKFTVFVTDLDNFGLLNQVFGEYFP
                     ESAPARSAVQVSSLPLDGLVEIEAIAVKRGK"
     gene            625..1509
                     /locus_tag="PLI55_09405"
     CDS             625..1509
                     /locus_tag="PLI55_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPB01085.1"
                     /translation="MPYKFAVIVNPVAGKGKTVHNLPLLKEIAAKTKSRFDYFITEGP
                     LHATKIADRIHQEYDAVVAYGGDGTANEVMNGLAGTETPIGVIPEGTGNDFARCINVP
                     HKFERAIQILDEYKTRLMDLGTIGNRVFINGVGVGFDAYVNVKTKSLKMLKGSMAYLY
                     SVLSSLAFWKSLPLAIEIDGCPISTDRSFLVAVGNGWACGGGLKLNPQANIHDSLLDV
                     CLIPEMPVWKLFLNLRRLKDGTISEVPGVLTMRCQAINISSEKPLPVHFDGEIFDAGA
                     GEVDIKIVPQSASVVGAW"
     gene            1528..2046
                     /locus_tag="PLI55_09410"
     CDS             1528..2046
                     /locus_tag="PLI55_09410"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPB01086.1"
                     /translation="MKRIFLVVGLLIILKSNLQSASPADTLMKISPKGAFIRSAIIPG
                     WGQVYNKKPVKGLVLLSAEGYFLYNFFYYNRIYDYVKTTKETLGIETWVGLTEEEKKA
                     QVLAVTGYELTLNSWRPREKRNKYGWWSLGTYIFCLLDAVVDAHLINFPKGEIELGST
                     EIGLNLSVRVNF"
     gene            2048..3403
                     /locus_tag="PLI55_09415"
     CDS             2048..3403
                     /locus_tag="PLI55_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPB01087.1"
                     /translation="MTKSREQWGSKIGFVLAAAGSAVGLGNIWRFPYLVGENGGAVFI
                     FIYLICVAVIGLPVIISEVIIGRNAQKDPVGSFKKLSNNNPFWKGVGFLGVFTGFIIL
                     SYYNVIAGWCLGYIVEAVKGSFQTITDTAQAVALFSRLSGSGSWSVPYLTIFMALTVG
                     IVYFGVTRGIEKSSKIMMPSLIIIILILVVRGITLENSKAGILYLLKPNFANVTGRTV
                     LVALGQAFFSLSIGMGVMITYGSYLSKENNIPSQATRIVVIDTSVSLLAGFMIFPAIF
                     ALGLKPHQGPSLLFNTSPILFNYIPGGYWFRILFFILLALAALTSTISLLEVITAYFV
                     DEFKLNRKMIAIIGGAISLLLGIPSALSFGRWSSFQIMNLSIFNFCDYLASNICLPIG
                     GLFITIFVGWFWGKNNALLEMRQGADRTPNWFFSVWRFLIRYVAPVLIAMVLLSSLGI
                     I"
     gene            3400..3759
                     /locus_tag="PLI55_09420"
     CDS             3400..3759
                     /locus_tag="PLI55_09420"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HPB01088.1"
                     /translation="MKNWWLLLLIFAIGGRLLAVPPVGEIIKSKPDDEWFAMDKLKHF
                     STSFYLTTTLFYSQNRIFDLKTDQANRNAAGLTISLGLLKEIRDLKQKENYFSWRDLA
                     ADILGAGAAILFINGIE"
     gene            3756..>4174
                     /locus_tag="PLI55_09425"
     CDS             3756..>4174
                     /locus_tag="PLI55_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPB01089.1"
                     /translation="MKTLNEYIGCIHIHTVYSDGSGTYEEVIRAAQEAGLDYIMVSDH
                     QTLRGRDEGYAGWHANLFVSIGYEIQDLEDRHHYLVFGIDKTLPADLTHQQYIKAVKA
                     AGALGIAAHPLEQRDKNYSLPGFPAITWSTLDYPEIEV"
BASE COUNT         1139 a          745 c         1019 g         1271 t
ORIGIN      
        1 tggttttcat gctccgacgg tttactttcc gctgattgtc aaagaggctt taatgattga
       61 gccaactgaa accgagagca aggaaactct ggatcgcttc atcgaagccc ttgtaaaaat
      121 agaccaggag gcaactaatg aacctgaaaa attacgtaat gctccctgga cgacgccggt
      181 cagacgttta gatgaattaa aagccaataa ggagttaaac atatgctggg ggaagtagag
      241 cgccaaattg tcaataccac ggatgcgccg cgtgccattg gaccttatag tcaagccgta
      301 atttccggaa attttgtatt tacggcggga caattaggac tcgatccagt cgctggaaaa
      361 attgtctcta atgattttga acagcaagtg cgtcaaataa tgcgcaacct aaaagcgatt
      421 ttacaggcgg ctgagagcga tttgaaaaat gtggtgaaat tcacggtatt tgtaaccgat
      481 ttagataatt ttgggctatt aaatcaggtt tttggcgagt attttccaga atcagcgcca
      541 gctcggtcag ccgtgcaggt tagtagcttg ccactggatg ggttggttga gattgaagcc
      601 atagccgtta aaaggggaaa ataattgccg tacaaatttg ccgttatcgt aaatccggtt
      661 gccgggaaag gtaagacagt tcacaacctg ccacttttga aggaaatcgc ggctaaaacg
      721 aaatcgcgct tcgattattt tattaccgaa ggaccgcttc atgctactaa aattgctgac
      781 cggattcatc aggaatatga tgccgtagta gcctatggcg gagatgggac ggctaatgaa
      841 gttatgaacg gtctggcggg aactgaaaca ccgatcggcg tgattcccga aggaacagga
      901 aatgactttg ctcgttgtat caatgtgccg cataaatttg aaagagccat ccagatttta
      961 gatgaatata aaacgcgctt aatggacctc gggacaatcg gcaatcgtgt ttttatcaat
     1021 ggggtgggag tgggttttga cgcctacgtt aatgtgaaaa ccaagagcct taaaatgttg
     1081 aaaggcagta tggcttatct atactcggtg ctatcgagtt tggcattctg gaagtcgctt
     1141 ccgttggcaa tcgaaataga cggctgtccg ataagcaccg atcgctcctt tttggtggct
     1201 gtcggcaacg gctgggcatg cggtggtggt ctgaaactta atccgcaggc taatattcat
     1261 gattcgttgt tggatgtatg cctgattccg gaaatgccgg tctggaaatt attcctgaat
     1321 ctaagaaggc taaaagacgg cacaatatcg gaagttccag gagttttgac gatgcgttgt
     1381 caagcaatta atatttccag tgaaaaacca cttccggtgc atttcgacgg ggaaatattc
     1441 gatgccggtg ccggagaagt tgatatcaaa atagttcctc aatcggcttc agtcgtaggg
     1501 gcttggtaat aggattaaaa gctggatttg aagcgtattt ttctggtggt aggattatta
     1561 ataatattga agagcaattt gcaaagtgct agcccagctg atacacttat gaaaatttca
     1621 ccgaaagggg catttatccg ttcggcaata attcccggct ggggacaagt ctataataag
     1681 aaaccggtca aaggtttagt actattgtca gcggagggat atttcctcta taattttttc
     1741 tattataatc gaatttatga ctatgtcaaa acgacgaagg aaacgctggg aatcgaaacc
     1801 tgggtagggt tgaccgaaga agagaaaaaa gcccaagtgc tggcggttac tggttacgaa
     1861 cttacgctga attcctggcg tcccagagaa aagcgtaata aatatggctg gtggagttta
     1921 ggcacttaca ttttttgttt actggatgct gttgttgatg cccatttgat aaatttccct
     1981 aagggagaga ttgaactggg tagcactgaa atcggactaa atttatcagt cagggtgaat
     2041 ttttaacatg accaagtcac gtgaacaatg gggctcaaaa attggatttg tacttgcggc
     2101 cgctggttcg gctgtaggac tgggaaacat ctggagattt ccttatttgg tcggtgagaa
     2161 tggcggtgca gtatttattt tcatctattt gatttgtgtt gcggttatcg gtttacccgt
     2221 cattattagc gaagttataa ttggacgaaa tgctcagaaa gatccagtcg gtagttttaa
     2281 aaaactatca aataacaatc ccttctggaa gggagtaggt tttctcggag tttttactgg
     2341 ctttattatc ctctcatatt ataatgtgat tgccggctgg tgtctgggat atattgtcga
     2401 agccgtcaaa ggttcatttc aaacgataac ggataccgcg caggcggtag cattatttag
     2461 tagattatcc ggcagtggca gttggagtgt accttacttg acaattttta tggcgctgac
     2521 ggtaggcatt gtatattttg gggttactcg tggcattgag aaatccagta aaattatgat
     2581 gcccagtttg attataatca ttttgatatt ggtagttaga ggaattacct tagagaattc
     2641 aaaggctggg attctctatt tgttgaaacc gaactttgcc aatgtcaccg gtcgaacggt
     2701 tttagtggct ctcgggcagg catttttttc acttagtatt ggtatggggg tgatgattac
     2761 ttacggtagt tatctatcaa aagaaaataa tatcccgtcg caagcaaccc ggattgtggt
     2821 aattgataca tctgtttcgc ttttagccgg atttatgatt tttcccgcaa tttttgcgct
     2881 ggggctaaaa cctcaccaag gaccaagttt attattcaat acttcgccga ttttatttaa
     2941 ttacattccg ggtggatact ggtttcggat tcttttcttt atcttattag ccttagccgc
     3001 cttgacctcg acgatttctt tactggaagt catcacggct tattttgtgg atgaatttaa
     3061 gttaaatcgg aaaatgatag caattatcgg aggtgccata agtttattac ttggaattcc
     3121 cagtgctctc tcctttggtc gctggtcgag tttccagatt atgaaccttt ccattttcaa
     3181 tttttgtgat tatttagcct ctaatatttg tttgccgata ggtggtttat ttataactat
     3241 atttgttggc tggttttggg gcaagaataa tgctttgtta gaaatgcggc aaggcgctga
     3301 tagaacaccg aattggtttt tttctgtttg gcgattttta attcggtatg tagcgccggt
     3361 tttgattgca atggttttat taagttcact cggtataata tgaaaaattg gtggctattg
     3421 ttattgatat ttgcaattgg cggtaggctt ttagccgtcc caccagttgg agaaataatc
     3481 aaaagtaaac cggatgatga atggtttgcg atggacaaac tgaaacattt cagcacctcg
     3541 ttttatctga ctacaacgct tttttatagt caaaaccgga ttttcgattt aaagactgac
     3601 caggctaatc gaaacgccgc cggcttgacg atttctttag gattattgaa ggaaataaga
     3661 gatttaaaac aaaaggaaaa ttacttcagc tggcgcgacc tggcggctga tattctgggt
     3721 gctggcgccg cgatattgtt cattaacgga attgaatgaa aaccttgaac gaatatattg
     3781 gttgtattca tattcatacc gtctattccg atgggagcgg gacgtatgaa gaagttattc
     3841 gagccgctca ggaagccgga ttggattata taatggttag cgaccatcaa actttgcgcg
     3901 gtcgagatga aggatatgcc ggctggcatg ctaatttgtt tgttagcatt ggttatgaaa
     3961 ttcaggattt agaagatcgc catcattatt tagtgtttgg tatagataaa accttaccgg
     4021 ctgatctgac tcatcagcaa tatattaagg cggtgaaggc ggctggtgct ctgggaattg
     4081 ccgctcatcc gctggaacaa cgtgataaga attattcatt acccgggttt ccggctatca
     4141 cctggagcac tctggattat cccgaaatag aagt
//
LOCUS       DAOOMU010000132         4171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_25686_length_4171_cov_6.458941, whole genome shotgun sequence.
ACCESSION   DAOOMU010000132 DAOOMU010000000
VERSION     DAOOMU010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25686_length_4171_cov_6.458941"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2573
                     /locus_tag="PLI55_09430"
     CDS             <1..2573
                     /locus_tag="PLI55_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB01090.1"
                     /translation="LPDANAAEAIGRLPGVSVLRSGGEANKVVLRGLSDKYSAITIDG
                     VRIASTDVDARGVDLSVISQGSLAGIELHKALTSDKDADAIAGAVNLVTKKAPEKRLL
                     RLDSKGSYNSLENSADQYDFNFRYGERFWHNLLGVQITANAEKRIRSREYYDVDYNKN
                     LNNWTDWEISDLALYYTDEIRKRQGAGLMLDFSTPDGGVIRFNNIYNFTERDYTEYYR
                     SYSSTEMAEYEVRDRIREIKAINSYIQGDNYILGLNLNWGLSYAESQTENPFDYEVTF
                     QEPSVTVGDSIIAGMRRVPVGFKGPLESLPDYAVNNFKKAYLYSAFYRTEEADEIDRN
                     VFLNISKKYTLSSSISGEIKTGGKYKDKSRSRSNTELFAPYYNVNYCYYERLEDGSIV
                     PKDFTGTAFENLETEGGKILTTNFLHETPRGRELFDLYRLYPLIDKNSMRELWELGEN
                     GVSDTLGSLPEYYENKEPNVHYYDINERITSGYLMNTFNFGQSVTWIAGLRVESEDNI
                     YKSRYCPTELSGFPVPSGPILDTTATHKEIVWLPNTHLNVRVTDFMSIRGAAYRALAR
                     PDFNRRLANYVARRRGTFYEGNNFTVGNPGLRAAKAWNYELNTSFYGDKIGLFSVSTF
                     YKDIEDMYHIMNGLVFEGTEVLDSLGIKFIPSYVKAGQPYALIYPYNSTKPTRVWGVE
                     VEHQANMRFLPGLLKNIVLNYNFSFVKSETWIPSVRIDTSWVTVPGLPFPMPKYIYRV
                     QEDKRKLEEQPEFFGNFAIGYDIKGFSIRLSMFHQGEFYRSYSPNRRSDLLQNSFTRS
                     DLAIKQQITKGISLMLNINNLTNTKEGTSLVDRIDHRTDINNEETYGLTADLGLRIEF
                     "
     gene            2692..>4171
                     /locus_tag="PLI55_09435"
     CDS             2692..>4171
                     /locus_tag="PLI55_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01091.1"
                     /translation="MKKLWVFQVFVVLTLLMTTSAFSQHELVVPPATGFSDVLNDVIA
                     GDTTATGERNDSARVYVLQRGGIYFVNSSIRNNGWPLNIIAQEGDGALPIIYQTPTSE
                     ATNGFDLIRIKGDCYVKDIVYVGYLESDPSTIAKIGNSFIRTDAAGYDIVVDNCILSQ
                     TQGQFFRTESAARLVKLTNCIFANMGDLGRSNFGAGKCVDFRGNSCDSAIFINNTFVN
                     FQDRIIRHRSSTAALNNLIFDHNTIINGFSYHGTLALGYVGDKVQITNNLWYDSFIAG
                     QDTDRVRQSEFDECQEQDDWGFSKMTWISSVPNDSTEWDVKFNYYAVSPDVQAFYDSV
                     GNVDQAFKGAGIPLTDHIAGKPGVEAFIKLESFDVVNRPEPMIAMAKWYRKPIANGGA
                     GKTKATDNFNRATDDYDRKDINYFLNDFDASYSTTSAAYTGASGGAPVGDLNWFPEFV
                     GVSKEKVTSPVSFMLKQNYPNPFNPTTQIAFDLDKSGYTTLTI"
BASE COUNT         1284 a          712 c          907 g         1268 t
ORIGIN      
        1 aattacccga cgccaatgcc gccgaggcta ttggcagatt accaggcgtg tcagttctac
       61 gatctggagg cgaagccaat aaagtagttc tccgtgggct tagtgataaa tattcggcga
      121 taacaataga tggtgtgcga attgcttcaa cagatgtgga tgcgagagga gttgatttga
      181 gtgtcatttc tcaaggtagt ttggcgggaa tcgaattgca taaagcttta acttccgaca
      241 aagatgctga tgctatcgct ggtgcggtta atcttgttac taagaaggcg cccgaaaaac
      301 gtctattaag attagattca aaaggttcat ataatagtct tgaaaatagt gctgaccagt
      361 atgatttcaa ttttcgttat ggcgagcgtt tctggcataa tctgctggga gtacagataa
      421 ctgcaaatgc tgaaaaacgt attcgaagcc gtgaatatta cgatgtagat tataataaaa
      481 accttaataa ttggaccgat tgggaaatct cagatttagc actttattac actgacgaaa
      541 taagaaaaag acagggcgca ggtctgatgc ttgatttcag tacgccagat ggaggagtga
      601 tccgttttaa taatatttat aattttaccg agcgcgacta tactgaatat taccgtagtt
      661 attcaagtac agaaatggct gaatacgaag tcagagacag gataagagaa ataaaagcta
      721 tcaatagtta tatacaaggt gataattata tacttggttt aaatttgaac tggggtcttt
      781 cctatgctga atcacagaca gaaaatccat ttgattatga agtaactttt caagagccgt
      841 cagtaactgt gggcgatagt atcattgctg gaatgagaag ggtaccagta ggttttaagg
      901 gaccgctgga atctttaccg gattatgctg taaataattt taaaaaagcg tatttatatt
      961 ctgcttttta tcgtacagaa gaggccgatg aaattgatcg gaatgtattt ttaaatattt
     1021 caaaaaagta taccctttcc agttcaatat ccggagaaat aaaaacagga ggaaaataca
     1081 aagataaatc ccggtcaaga tctaacactg aactttttgc cccgtactat aatgttaatt
     1141 attgttatta tgagagatta gaagatggta gtattgtacc aaaggatttt actgggaccg
     1201 catttgaaaa tttggaaact gagggtggca agatattaac tacaaatttt ttgcatgaga
     1261 ctccccgggg cagagaatta tttgatttat atcgtctata tccgctaata gataaaaatt
     1321 caatgaggga attatgggag cttggagaaa acggtgtctc ggatacacta ggctcactac
     1381 ccgaatatta tgaaaataag gaaccaaatg tacattatta tgatattaat gaacgaatta
     1441 cctccggtta cctaatgaat acttttaatt ttggtcaaag tgtaacctgg attgctgggc
     1501 ttcgagttga atctgaagat aatatttata aatctcgata ctgtccgacc gaattaagtg
     1561 gatttccagt tccaagtggc ccaattttgg ataccacggc aactcataaa gaaattgtgt
     1621 ggttacctaa tactcatctc aatgtgagag ttacagattt tatgagtatt cgcggtgcgg
     1681 cttaccgagc attagcccgt ccagatttca atagacgact agctaattac gtagcccgta
     1741 ggcgaggtac tttttatgaa gggaataatt tcaccgtcgg caatccaggt ttaagagccg
     1801 caaaagcctg gaactatgaa ttgaatacgt ctttttatgg agacaagatc ggtctgtttt
     1861 ccgtttcaac tttttataag gatattgaag atatgtacca tataatgaat ggtctggttt
     1921 ttgaaggcac cgaagtttta gatagccttg gcataaaatt tatcccgagt tatgtcaagg
     1981 ctggccaacc ttatgctttg atttatcctt ataattcgac caagcctacc cgagtctggg
     2041 gagttgaagt tgaacaccaa gctaatatgc gctttttacc tgggttattg aagaacattg
     2101 tgcttaatta taacttttca tttgttaaat ccgaaacatg gattcccagt gtaagaatag
     2161 acacctcatg ggttacggtc ccgggcttgc cttttccaat gcctaaatac atttatcgag
     2221 ttcaggaaga taaacgaaaa ctggaagaac agccggaatt cttcggcaat tttgctattg
     2281 gctatgatat aaaaggattt tcgatccgct tatcaatgtt tcatcaaggt gaattctatc
     2341 gatcgtattc gcctaatcgt cgatccgatt tgcttcaaaa tagttttact cgttcggatt
     2401 tggctatcaa acagcaaatc actaaaggaa tttcccttat gttgaatata aataatttaa
     2461 caaatactaa ggagggtact tcattagtgg atagaataga ccatcggact gacattaata
     2521 acgaagagac ctatggcttg actgccgacc tgggacttag aatagaattt taagtttaat
     2581 aagaaaataa ttgattcaaa atattagaaa agaggtgata agaatatgat ttaaaatgtc
     2641 caaacgtata tactaaagaa actaacccgt aactaacaaa aggagactat tatgaaaaaa
     2701 ttgtgggtat ttcaggtgtt tgtcgtgctg actttgctaa tgacaacttc ggctttcagc
     2761 cagcacgagt tggttgtgcc gcctgcgaca ggatttagtg atgtgctaaa tgatgtaatt
     2821 gcaggagata ccaccgcaac aggggaacgg aatgattctg ccagggtata tgttttacaa
     2881 cgtggtggaa tttatttcgt caattcttcg attcgcaata atggttggcc attaaacatt
     2941 attgcgcagg agggagatgg ggctttacca atcatttatc aaaccccaac ttctgaagcg
     3001 actaatggat tcgatttgat tcggatcaaa ggtgattgtt atgtcaaaga catagtatat
     3061 gtaggatacc ttgaatccga cccaagtaca atcgcgaaga ttggtaattc ttttataaga
     3121 acagatgcag ctgggtatga catagtggtt gataattgta ttttatctca aactcagggt
     3181 caatttttcc gaactgaaag cgcggcgcga ctggtaaaat tgactaattg tattttcgcc
     3241 aatatgggtg atttaggtcg ctctaacttt ggtgctggta aatgtgttga tttccgtggg
     3301 aattcttgcg attctgcgat ttttattaac aatacttttg tcaatttcca ggatcggatt
     3361 attcgtcacc gctccagtac cgcggcattg aacaatctta tttttgatca taatacgatt
     3421 attaacggtt tttcatatca cggtacatta gccttaggtt atgttggaga taaggtgcag
     3481 attaccaata atctctggta tgattccttt atagctggcc aggacactga tagagttcgg
     3541 cagtcggaat ttgatgaatg tcaagaacaa gatgattggg gtttttctaa aatgacctgg
     3601 atttcttcgg taccgaatga ttcaaccgaa tgggatgtaa aatttaatta ctatgccgtt
     3661 tcaccagacg ttcaagcctt ttatgatagt gttggtaatg ttgatcaagc atttaagggt
     3721 gccggtatac ctttaaccga tcacattgcc gggaaacctg gcgtggaagc atttattaag
     3781 ttggaatcct ttgatgttgt gaatcgtcca gaaccgatga tagctatggc aaaatggtat
     3841 cgtaaaccaa tagctaatgg tggagccggg aaaaccaaag caacggataa cttcaataga
     3901 gcaactgatg attatgatcg aaaagacatt aattactttc tgaatgattt tgatgccagc
     3961 tatagcacca cgtcggctgc ttataccggc gcttctggcg gagctcccgt cggtgatctg
     4021 aattggttcc cggaatttgt tggcgtgagt aaggaaaagg tgactagccc ggttagtttt
     4081 atgctcaagc agaactatcc gaacccattc aacccaacta ctcagattgc atttgatctg
     4141 gataaatccg gttatacgac tctaacgatc t
//
LOCUS       DAOOMU010000133         4129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_26028_length_4129_cov_18.747914, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000133 DAOOMU010000000
VERSION     DAOOMU010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26028_length_4129_cov_18.747914"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2246)
                     /gene="nifJ"
                     /locus_tag="PLI55_09440"
     CDS             complement(<1..2246)
                     /gene="nifJ"
                     /locus_tag="PLI55_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006488153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPB01092.1"
                     /translation="MSKTMKTMDGNTAATHIAYALSEVAAIYPITPSSVMGELADEWS
                     AQGRKNIFGQPLRVVEMQSEAGAAGAVHGSLSAGALTTTFTASQGLLLMIPNMFKIAG
                     ELMPAVFHVSARAVAGQALSIYGDHSDVMATRTTGFGLMASGSVQEVMDLSLVTHLAS
                     LKASLPFVSFFDGFRTSHEIQKIEVIDYETIKSLVDFDDIDRFRARCLNPEHPQLRGT
                     AQNPDIFFQVAELANKYYDAVPEIVAEMMRKVGEVTGRHYNLFDYAGDPNAERVVVIM
                     GSGSETVQGVAEYLNARGEKVGVLKVHLYRPFSAKHFMAALPKSAKRIAVLDRTKESG
                     AFGEPLYTDVCAVLQREKDNRLVVGGRYGVGQKEFSPAMAKAVFDNLKLPEPKNGFTV
                     GIEDDVTFRSLPVTEDIDTVPEGTVQSIFWGFGSDGTVGANKDAIKIIGDNTDLYAQG
                     YFSYDSKKSGGLTVSYLRFGPKELHTPYRINLADYVACHKQSYINNYDMLVNLKPGGI
                     FVLNTLWDDAELDANLPAAMKRAIATKKVRFYAVDANRIAGEVGLGGRINMVMQTAFF
                     ALSKVLPFEEAIKHLKNAIQKTYGAKGEKIVKMNWQAVDSAINAIREVKYPASWAEAK
                     ETAESPKPVPDFVKKVLQPINAFNGDNLPSSVFTPGGIFPTATTKYEKRGIAIEVPEW
                     IKENCTQCNQCSFVCPHAVIRPVLVTPEEKAKAPTTFETIKAIGKGLENYEYRMQVSV
                     LDCTGCGN"
     gene            complement(2680..3870)
                     /locus_tag="PLI55_09445"
     CDS             complement(2680..3870)
                     /locus_tag="PLI55_09445"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPB01093.1"
                     /translation="MKKRIGILTGGGDVPGLNPAIRGITLRALKEGFEVIGIRRGWAG
                     MINIIRDKEADNSQNFIMLNQEIVNKAGRTGGTFLHTSRTRPSSVKLDAVPEHLRDKY
                     TAEVNDLTPEVLKNLDFLGIDYLIPIGGDDTLSYGVTLHKAGVKVIGVPKTMDNDVPG
                     TDYCIGFSTCVTRTLELTHSLRTSAGSHERFLVIEVFGRYAGFTALLPTLAGAANRCV
                     IPEYKFNMERLTELLVQDRFTNPSRYSVVLVSEGAMMQNQDQMMFQSEETDAFGHKKL
                     GGIGDVVARQLKELSGKYNNGHKINVISQKLGYLVRCGDPDALDSIVPMAYGNIAFEL
                     IMNGRSGLLVNLRNGVYGHTDLEVVTSTKKVVDVEKFYETDRLRPKYKSFENKSFFIM
                     TSDK"
BASE COUNT         1168 a         1016 c          825 g         1120 t
ORIGIN      
        1 cagttaccgc aaccggtgca atccagcacc gagacctgca tacgatactc gtaattttcg
       61 aggcctttgc ctatggcttt aatggtctcg aaagttgtag gcgccttggc tttttcttcc
      121 ggagtgacca gtaccggacg aattacagca tgtgggcaga caaaactgca ctgattgcat
      181 tgggtacaat tttccttgat ccattctgga acttcgatgg cgattccacg cttttcatat
      241 ttggttgttg ccgtcgggaa aatgcccccc ggcgtgaata cgctacttgg cagattgtca
      301 ccattaaagg catttatcgg ctgtaaaact tttttaacaa aatccggtac aggttttggc
      361 gattcggccg tctctttggc ttccgcccaa gacgctgggt attttacttc acgaatggca
      421 ttgatggcag aatctacagc ctgccagttc atcttgacga ttttttcacc cttggcaccg
      481 taagttttct gaatagcatt ttttaagtgc ttgatggctt cctcgaaagg caaaactttg
      541 gacagcgcga agaaggctgt ctgcattacc atattgatgc gacctccgag tcccacttca
      601 ccggcaattc tattggcatc taccgcatag aaccgaactt tcttggttgc gatcgcccgt
      661 ttcatagcgg ctggcaaatt agcgtccagc tcggcgtcat cccaaagcgt attcagtacg
      721 aaaatgccac ccggtttaag attaaccagc atatcgtaat tattgatata actctgttta
      781 tgacaggcga cataatctgc cagattaatc cgatagggtg tatgtaattc ttttggtccg
      841 aaacgcaaat agctgaccgt caaaccaccc gattttttcg aatcgtaaga gaaatagccc
      901 tgcgcgtata aatcggtatt gtcgccgata attttaatcg cgtccttatt ggcaccgacc
      961 gttccatccg agccaaaacc ccagaaaata gattggacgg taccttccgg cacagtgtca
     1021 atatcttcgg taactggcaa actgcggaag gttacatcat cttcgatgcc caccgtaaaa
     1081 ccgtttttcg gctcgggcag tttcagattg tcgaaaacgg cttttgccat cgccggactg
     1141 aattcttttt ggccaacgcc gtagcgcccg ccaacgacta agcgattatc tttttctcgc
     1201 tgtaaaacag cacacacatc ggtataaagc ggttcaccaa aagcgccgga ttctttggta
     1261 cgatccagca cagcaatccg cttagcactc ttgggcaaag ccgccataaa atgcttagcc
     1321 gagaaggggc gatacaggtg aacctttaat acgccgactt tctcacctct ggcatttaag
     1381 tattcagcaa caccctgaac ggtctcggac ccggaaccca ttataaccac cactcgctcc
     1441 gcattcggat caccggcata atcgaatagg ttataatgac ggccggtaac ttccccaact
     1501 ttccgcatca tttcagcgac gatttcggga acggcatcgt aatatttatt agccaattca
     1561 gctacttgaa aaaagatgtc cggattctga gccgtgccac gtaattgcgg gtgttctgga
     1621 ttcaaacagc gggctcggaa acgatcaatg tcatcaaaat caactaatga cttaattgtt
     1681 tcataatcta tgacctcgat tttctgaatt tcatggctgg tgcgaaaacc atcaaagaaa
     1741 ctgacaaacg gcaggctggc tttcagcgaa gctaaatgag taaccagtga taaatccatc
     1801 acctcttgca ccgatccgga tgccattaaa ccaaaaccgg tcgtgcgagt agccataacg
     1861 tccgaatgat caccgtaaat cgagagtgct tgcccggcta ccgctcgcgc cgaaacatga
     1921 aacacggctg gcatcaattc accagcaatc ttaaacatat taggaatcat taatagaaga
     1981 ccctgcgaag cggtaaaggt tgtagtcagc gcgccagctg aaagactgcc gtgcacggct
     2041 cccgccgcac cggcttcact ctgcatttca accactcgca ggggctgtcc gaaaatattt
     2101 ttccgacctt gcgccgacca ttcgtcagcc agttctccca tcacagacga tggtgtaatc
     2161 ggataaatag ccgcaacttc actaagagca taggctatat gagttgccgc tgtattgcca
     2221 tccatagttt tcatagtttt agacatggtg attctccatt gaatatttta atgtttataa
     2281 aattatttga attattgctg ggtttttttg aattagtaaa ttcatcactt tcaaaactaa
     2341 agaaagttac gaccgaaatg cccagaaaag caaccgctat ctctataagg gaggtaattg
     2401 cagagccaca cgtatttttt cggaaaaaaa tgaagggaat attttcaatt agtcttaaaa
     2461 agggaggaaa gattgaccct tcttaaccta atgctatcca tttcatcatc ataatcagct
     2521 tcaacaccat aaatcagtta aaggataaat tatggttcca ataaaaaagt gaagcccaaa
     2581 aaatatcacc ttgccgctcc ttttaaaagc aaaaagggca atcatgcgat tgcccttttg
     2641 cggtgtcctc atagagagga gggaggaggg aaactttgtt tacttatcgc tggtcatgat
     2701 aaaaaacgat ttgttttcaa aactcttata cttaggacgc aggcgatcgg tttcataaaa
     2761 tttttcaaca tctaccactt ttttggtgct agtaaccact tccaggtccg tatgaccata
     2821 gacaccatta cgcaagttga ccagcagtcc ggaacgacca ttcataatca actcaaatgc
     2881 gatattacca taagccatcg gcacaatcga atcgagggca tctggatcac cgcatcgcac
     2941 caaataaccg agtttctggc tgatgacgtt aattttatga ccgttattat attttccgga
     3001 tagttcttta agctgccttg caactacatc cccgattccg cccagttttt tatgaccaaa
     3061 ggcatcagtc tcttcgctct ggaacatcat ctgatcctga ttctgcatca tagcaccttc
     3121 tgaaactaga accaccgaat agcgactggg attagtaaag cgatcctgca ccaatagctc
     3181 ggttaagcgc tccatattga atttatactc gggaattaca cagcggttgg cagccccagc
     3241 tagagtcgga agcaaagccg taaaaccggc atagcgtccg aaaacttcga ttaccagaaa
     3301 acgttcgtgt gaaccggctg aagtccgcaa actatgagtc aactccagcg tccgcgtcac
     3361 acaagtactg aaaccgatac aataatccgt accggggaca tcattatcca tagttttggg
     3421 aacaccaatc accttcaccc ccgctttatg caaagtgacg ccgtagctca gggtatcgtc
     3481 cccgccgatc ggaattagat aatcaatccc gagaaaatcg aggtttttaa gtacttcagg
     3541 ggtcagatca ttaacctcag ctgtatattt atcccgcaaa tgctccggta cagcatccaa
     3601 tttaacgcta cttggccgcg tgcgggaagt atgtaaaaat gtcccaccgg tgcgcccggc
     3661 tttattgacg atttcctgat tcagcatgat gaaattctgg ctgttatcag cctctttatc
     3721 tcgaatgata ttgatcatcc ccgcccagcc gcgtcggata ccaataacct caaagccctc
     3781 ttttaatgcc cttaaagtaa tgccgcgaat tgccggattt aaaccaggta catcgccgcc
     3841 acctgtcaat attccgattc tctttttcaa ttaatgctcc ttatattgtt tcaactaaaa
     3901 cagatcatca aatcggattc aattttaaca taaattaagc aaattttcaa caatcttatt
     3961 aaactttgga tttaggattt ttcttaaaat gaataaaacc tattgcccgc cagaatatcc
     4021 ataaaattac gccatataca attataagcc cgaatgtaat ccagtatcct ttgaggctat
     4081 tattgaaaaa attcatcggc acatttagca gtattaaaag tggcaaacc
//
LOCUS       DAOOMU010000134         3992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_27268_length_3992_cov_3.865634, whole genome shotgun sequence.
ACCESSION   DAOOMU010000134 DAOOMU010000000
VERSION     DAOOMU010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27268_length_3992_cov_3.865634"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLI55_09450"
     CDS             <1..609
                     /locus_tag="PLI55_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01094.1"
                     /translation="VTFKIEKRFSQNYSFRADYTYQTAEGTYSDPTDAFNSALNNEQP
                     VLALVPMEWDQRHTVNAQIIYDISGWTFSLIGRYWSGRPYTPSFPSSETVGGAAVSGL
                     TTNSARRPSQRNIDLTLNKILMVKSKVRLELFANIYNVLDIRDELNVYTDTGTADYTT
                     TINPKKVVYSPDRVSTVEDYIKQPTWYTSPRQIQVGLAIGFN"
     gene            628..>3992
                     /locus_tag="PLI55_09455"
     CDS             628..>3992
                     /locus_tag="PLI55_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01095.1"
                     /translation="MNKKIFILFIILGCFFTSGLFAQMTYLHGTDNEFRMGLHAGNQF
                     RTTFYNDGTYGAINPWTPDEIIGEWPINSSHYYLMDGNIFVGSEVIDTNNQLIHILSE
                     NKSYNTGGSTGDKRPDGSWRTFLPLPGFANQSSDRIAMAKGGSEWPDSWPSYWPDIAD
                     PNNPYGIYDPDGWAGCWNGYFGKNVFNADEESYFVADDYENDEFAFFPDANNPNRRGL
                     GLRIYVRGFQWAKAPVEDGIFCLFDIENIGTHLHNKMVFGYKIGNNIGMCSENDQDTG
                     DDNAAFDRELDLAYMWDNDLIGGACWGPDPVGYMGGAFLESPGNPYDGIDNDNDGIGG
                     SGKTITEDLFAPKVLTLSDQIVLINYDTYDRIVTTLRDTLTQLGKNSTDTLSITFNNA
                     TFRYWAGKTLTEDPTGSNLFDDNLNGLVDENRKPEGATQALYLGYKYVDYFTGEGLNN
                     PLIDERRDDGIDNDNDWDIIYDDLGVDGIGPNSRDYDGADIGEGDGQPTAGEPHFDKT
                     DIDETDMLGLTSFNLYEWTSINQNDDEAYWQAMSPGYFSNNMTGSNVELLYGCGYFPL
                     SPGQVERLSIAIICGTNYNDLVRNKNNFADAYNRNYNFAKAPNIPTVRAVAGDNKVTL
                     FWDTFAEDSDDPITGKDFEGYKIYRSTDPGWNDCTPITDGYGSVIFREPLAQFDLDNG
                     IYGFADIPTQGVHFWLGNDTGLRHYWVDTTAVNGYTYYYAVTSYDHGTQQPSGSLIID
                     SLKIDPSECSKFISIAASGTVEKGSNVAIVRPEAPSAGYKPAEMESGIIPGPNNTATG
                     TINYTIYDNTNIKNNHQYQITFKDSLSGTGVTATKSTKSFTLVNTTTNDTLIYDHSLE
                     GGAEGLPIVDGFQLAFANNPEKLEVDMDRSGWNTPGIPEFRFRPENKAKPEDLYTSDF
                     KIAFRDTPDPSLPKPIFYYKGSSSKLYEPIPVNFEVISLKENKNIPFAFREKHATSTA
                     DSGIFSFDLTSTVKYDEIIFLSPDSLPSWKAIYYITATTKDSLVPGTGDTLTIRLKFP
                     FLSHDVFEFTSKVDTIDVEKAKNDLSKIRVVPNPYVVTNTWEPKNPYSSGRGERQLHF
                     IHLPAKCTIKIFNVRGQLVDTIKRDAQSDDGTE"
BASE COUNT         1196 a          758 c          874 g         1164 t
ORIGIN      
        1 gtgaccttca aaatagaaaa acgatttagc caaaattatt cctttagagc tgattacacc
       61 tatcaaacag cggaaggaac ctattccgat cctactgacg ctttcaattc ggctttaaat
      121 aacgaacagc cggttttagc actagtgcct atggaatggg atcaaagaca tacggtaaac
      181 gcccagatta tctacgatat ttccggctgg acattttcac tcatcggacg ttattggagt
      241 ggcagacctt atactcctag tttcccgtct tcggagacag taggaggtgc ggctgtatcg
      301 ggattaacca cgaatagcgc tcgccgacct tctcaaagaa atattgatct gacactcaat
      361 aaaattttaa tggttaagtc taaggttagg ttagaattat tcgccaatat ttataatgtg
      421 ctcgatatta gagatgagtt aaatgtttac accgatactg ggacagccga ttatactact
      481 actatcaatc ccaagaaggt tgtatatagt ccggaccggg taagcacggt cgaagattat
      541 attaaacaac caacttggta cacttcgccg cgccagattc aggtcggttt agccataggt
      601 ttcaattgag aatcaggaga ttaataaatg aataagaaaa tctttatcct atttataatt
      661 ttaggatgtt tttttacttc gggtttattt gcccagatga catacctgca tggaactgac
      721 aacgaatttc ggatgggact acatgcgggc aatcagttcc gcactacttt ttataatgat
      781 gggacttatg gagccatcaa tccgtggact ccggatgaaa tcatcggtga atggccaatt
      841 aacagttctc attattactt aatggatggc aatattttcg tcggctcgga agtaatagat
      901 actaataatc aactaattca tattttaagt gagaataaaa gttacaatac cggcggctca
      961 accggagata aacgaccgga cggctcctgg cgcacttttc tacctttacc tgggtttgct
     1021 aatcaatcga gcgaccggat cgcaatggct aaaggcggtt ccgaatggcc tgattcctgg
     1081 ccttcatatt ggccggatat agccgatccc aataatccat atggtattta tgatccggat
     1141 ggctgggctg gttgctggaa tggatacttt gggaaaaatg tattcaatgc cgatgaggaa
     1201 agttactttg ttgccgatga ttatgaaaat gatgaatttg cctttttccc ggatgccaat
     1261 aatccaaatc ggcgtggttt gggattaaga atctatgttc gaggatttca atgggcaaaa
     1321 gctccggtcg aagatgggat attttgtctt tttgatattg aaaatattgg tacgcattta
     1381 cataataaaa tggtcttcgg ctataagatc ggcaataata tagggatgtg cagtgaaaat
     1441 gaccaggata ccggagatga taacgccgca tttgaccggg aattggattt agcttatatg
     1501 tgggataatg atttgatcgg aggcgcatgc tggggaccag atccagtcgg atatatggga
     1561 ggtgctttcc tagaaagtcc tggcaatcct tacgacggca ttgataacga taatgacgga
     1621 ataggtggct caggcaagac tattacagaa gatttatttg cacccaaagt cctcactctg
     1681 agtgatcaaa tcgttttaat caattatgat acctatgata gaatcgtcac gactttgaga
     1741 gatactctaa cacagcttgg gaaaaattcg accgatacgc tttcgattac ttttaataat
     1801 gcgacttttc gctattgggc gggcaagact ttaaccgagg atccgaccgg ctccaattta
     1861 tttgatgaca acttaaatgg tctggttgac gaaaatagaa aaccggaagg agccactcag
     1921 gctctttatt taggttataa atatgttgat tattttacgg gtgaagggct caataatcct
     1981 ttgattgatg aacggcgtga cgatggaatt gacaatgata acgactggga tattatctat
     2041 gatgatctgg gggtggatgg aattgggccc aattcccgtg actacgatgg tgcagatatt
     2101 ggcgaaggtg atggacaacc tacagccggt gaaccacact tcgataaaac tgatatagat
     2161 gaaacagata tgctgggatt aacgagtttt aatctttatg aatggactag tattaaccag
     2221 aatgacgatg aagcatattg gcaagcgatg tcgcccggct atttttccaa caatatgacc
     2281 ggttcgaatg tggaactgtt gtatggttgt gggtatttcc ccttatcgcc gggtcaggtt
     2341 gaaagactgt ctattgcgat tatttgtggt actaattaca atgatttagt acgcaataag
     2401 aataattttg cagatgctta taatcgtaat tacaattttg ctaaagcccc gaatatccca
     2461 accgttcgag cggtagcagg tgataataaa gtaactctgt tctgggatac cttcgctgag
     2521 gattccgacg atcccatcac cgggaaagat tttgaaggtt ataaaattta tcgttcgact
     2581 gatcccggtt ggaacgattg taccccgatt actgacggtt atgggagtgt aattttccgg
     2641 gagccactgg ctcaatttga cttggataac ggaatttacg gttttgccga tattccgact
     2701 cagggagtgc atttctggtt gggaaatgat actggcctcc gtcattattg ggtagatact
     2761 acggcagtaa atggttatac ttattattat gctgtaacct cttatgacca cggaacacaa
     2821 caaccctccg gttcgctgat aattgactct ctaaagattg acccttcaga atgcagcaaa
     2881 ttcatatcaa ttgccgcttc tggaaccgtg gaaaaaggtt caaatgttgc gattgtcaga
     2941 ccggaagcac catcggctgg gtataagccg gcggaaatgg aatctggaat catacctgga
     3001 ccaaataata ctgctactgg cacaattaat tacaccatct atgacaatac taatattaaa
     3061 aataatcatc aatatcaaat cacttttaaa gattctctaa gtggaacagg tgtcacggct
     3121 acaaaatcta ccaaaagttt cactttggtc aatacgacta ctaatgatac attgatctat
     3181 gatcattcgc tagaaggtgg cgccgaaggc ttaccgattg tggacggatt tcaattagct
     3241 tttgccaata atccggagaa gttagaagtg gatatggatc gttcgggatg gaatacgccc
     3301 ggaattcccg aatttagatt ccgaccggaa aataaggcta aacccgaaga tttatataca
     3361 agtgatttta aaatagcttt tcgggatact cctgatccat ctctaccaaa gccaattttt
     3421 tactataaag gcagtagtag taagctatat gaaccaattc cggttaactt cgaggtaatt
     3481 agtcttaaag aaaataaaaa tatcccgttt gcctttcgtg aaaagcatgc aacttctaca
     3541 gccgattcag ggattttttc tttcgattta accagcactg tcaaatatga tgaaataatt
     3601 ttcctgagtc ctgattcgtt acctagctgg aaagctattt attacatcac ggcgacgact
     3661 aaagactcct tagtacccgg cacaggtgat actttaacta ttcgattgaa atttccattc
     3721 ctctcgcatg atgtttttga atttactagt aaagttgata ctattgacgt cgaaaaagca
     3781 aaaaacgacc tgagtaaaat tagagtagtt cccaatccat acgtcgtgac gaacacttgg
     3841 gaacctaaaa atccttattc ttcagggcgt ggtgaaaggc agctccattt tattcattta
     3901 cctgccaagt gtactattaa aatattcaat gtcagagggc agttagttga tactattaaa
     3961 cgagatgccc agagtgatga tggaacggaa at
//
LOCUS       DAOOMU010000135         3973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_27425_length_3973_cov_16.555896, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000135 DAOOMU010000000
VERSION     DAOOMU010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27425_length_3973_cov_16.555896"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1335)
                     /locus_tag="PLI55_09460"
     CDS             complement(139..1335)
                     /locus_tag="PLI55_09460"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPB01096.1"
                     /translation="MSNNIFLKILSICSILVLLIQSSAISQVILDPDAVVEEIVSGIQ
                     QPEGPVWREGLGLLFSDIKGNKIYKWTSEKGKEIYLDPSYNTNGLAYDLEGRLVAGQM
                     GLRRVVRFEKDGSQTSLADSYDGKKLNSPNDLVIKSDGAIFFTDPDFNIPSGESKELN
                     FNGIYRISPSGVLQLLTSSLTLPNGICFSPDESKLYVNDSQVHKIYVWDVVDDSTITN
                     KKLLYTIPVPFGYADGMKIDEDGNIYCTCSSYVWVISPEGELIGKIDLPTSVSASNCA
                     WGDDDNRTLFITAGNSVYRVRPFVTSINKEDSSLPKSFGLSQNYPNPFNPTTKITYSI
                     PVTSNVMITVYDVSGKEVVTIEDCQKKAGTYTLLFNAEKLASGVYYYNLTANEFSSTK
                     KFVILK"
     gene            complement(1488..3953)
                     /locus_tag="PLI55_09465"
     CDS             complement(1488..3953)
                     /locus_tag="PLI55_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HPB01097.1"
                     /translation="MAFALIISSCAKKPDRNSDSADNPMKLWYQQPAKQWVEALPIGN
                     GRLGAMIFGDPLREEIQLNENTIWGGKPHRNDNLEAKEALPKIRQLIFNGKYKEAQEL
                     ADQKFISKNSQGMPYQTAGSLYLSFPEHKNFTNYYRELNIETAVVTTRYEVDGVTYKR
                     EVFASFPDQVIICRISTSKPGKINFTASMNHPGTVNIFTEGNDILIMSGITSDCDSIK
                     GEVKFQVQVKVTTESGSIEASQNSLTVNNADIATIYISMASSFKNYSDISENAGEKAQ
                     SYLRNALRKNYKQALENHIADYQRYFKRVSINLGVADSIKNPTDVRIERFAKGNDPQL
                     VALCFQFGRYLLISSSRPGTQPANLQGIWNNQLYPPWDSKYTVNINTEMNYWLSEPTN
                     LSEMNEPLVQMIEELSETGRQTAESMYGAEGWVLHHNTDIWRICGPVDGAFWGMWPMG
                     SAWLCQHLFEKYEYSGDREYLKSVYPAMRGAVEFYLDFLVEEPQYKWLVVCPSVSPEN
                     APACHPEFSITAGTTMDNQLLFDLFTKTIKVAEILNTDKELVIKIKETLHQLPPMQIG
                     RWGQLQEWMQDWDDPEDHHRHVSHLYGLYPSNQISPYRTPELFSAAKTSLLARGDEST
                     GWSMGWKVNLWARLLDGDHALKLITNQLSPSIQPDGSQKGGTYPNLFDAHPPFQIDGN
                     FGFTAGVVEMIVQSYDGFIFPLPALPEIWKSGSITGIRLRGGFEIVRLEWQNGKISTL
                     TIKSNLGGNCRIRSYDKLIPVGNFKLEEAKGENPNPFYAISQVKEPLISPDANIIPDE
                     LRKSHLYDFNTKAGKEYTLILLE"
BASE COUNT         1201 a          821 c          691 g         1260 t
ORIGIN      
        1 tgggagtaca aaatagtact gctcattaaa cacaataata atgctgtaca tagtgacttt
       61 ctgattaatt tttccatgct tacgctccta ttaattggtt atatttttta attaataatc
      121 tcaaatgacc acctaaaact attttaatat cacaaatttt ttggtggatg aaaattcatt
      181 cgcggtaaga ttgtagtaat atactccact cgctaatttc tcagcattga acaatagagt
      241 ataagtcccg gcttttttct gacaatcctc tatcgtcact acttcttttc ccgacacatc
      301 ataaaccgtt atcattacat ttgaagtaac tgggattgaa taagtaattt ttgtagtcgg
      361 attgaaggga ttaggataat tctgagaaag tccaaaactc ttaggtaaac tcgagtcttc
      421 tttattgatt gaagtaacaa acggacgcac tctataaacc gagttaccag cggtaatgaa
      481 taatgtccta ttgtcatcat cgccccaggc acaatttgaa gcagaaacac tcgttggtaa
      541 atcaattttc ccaattaatt cgccctcagg agaaataacc caaacataag acgagcaggt
      601 acagtaaata ttaccatctt catcaatttt catcccgtcc gcatagccga atggtacggg
      661 aatagtatat aatagttttt tattggttat ggtagaatca tcaaccacat cccatacata
      721 aattttatgt acttgagaat cattgacata gagtttggac tcatcaggag aaaagcagat
      781 accattaggc aaagtcaatg acgacgtcag aagctgaaga acacctgaag ggctgattct
      841 ataaattcca ttgaagttga gttccttgct ttctcctgat ggtatattaa aatctggatc
      901 ggtgaagaaa atcgcaccat cagatttaat aaccaaatca tttggactgt ttaatttctt
      961 tccatcataa ctatctgcca gcgatgtttg cgagccatct ttttcaaaac gaacaactct
     1021 tctaagcccc atctgcccgg caacaaggcg accttccaaa tcataagcta atccattggt
     1081 gttataagaa ggatctaaat aaatctcctt gcctttttcg gaagtccatt tataaatctt
     1141 gtttccctta atatcagaaa ataataaacc gaggccttct ctccaaaccg gtccttccgg
     1201 ttgctgaatt ccagaaacta tctcttcaac tacggcatct ggatcaagta ttacttgaga
     1261 aatagccgac gattgaatca ataggaccaa aatgctacaa atagatagaa tctttaaaaa
     1321 aatattatta ctcattttcc tctcactttt acattaaaat tggactaata taaatactac
     1381 cagagatata catttattaa atctatcatt aatactagtt ttaattctct aaacgctaat
     1441 cggatggtaa acttttcggg caagattctt ttcataaatt ttaatcatta ctctaataag
     1501 atcaaagtgt attcctttcc agctttagta ttaaaatcat acagatgact tttgcgaagt
     1561 tcatcaggaa taatattcgc atcaggagaa atcaaaggtt cctttacttg agatatagca
     1621 taaaatgggt tgggattttc cccttttgct tcttcaagtt taaaattgcc cacaggaata
     1681 agcttatcgt agcttcttat tctacaatta ccgcctaagt ttgattttat agttaacgtg
     1741 gaaattttac cattttgcca ttctaatctc acaatttcaa atcctccgcg taaacgaata
     1801 ccggtaatac ttccgctttt ccaaatttca ggtaatgccg gcaatggaaa gataaaacca
     1861 tcatagcttt gtacaatcat ttccacaacg cctgccgtaa atccaaaatt tccatcaatt
     1921 tgaaaaggtg gatgagcgtc gaacagattt ggatatgtcc cgcctttttg gcttccatct
     1981 ggttggattg atggagataa ttgattggta atcagtttca aagcatgatc gccatcaagg
     2041 aggcgagccc ataagttcac tttccacccc attgaccaac cggtggattc atcgccgcga
     2101 gctaataaag aagttttggc ggctgaaaat aattccggtg ttcgataggg cgatatttga
     2161 ttggatggat ataggccata caaatgcgaa acatggcgat ggtggtcttc aggatcatcc
     2221 caatcctgca tccattcttg caactgcccc cacctgccaa tttgcattgg cggcaattga
     2281 tgcaaggttt cttttatttt tatgacaagt tctttgtcag tgttgagaat ttccgccacc
     2341 ttaattgttt tagtaaataa gtcaaaaagg agctgattgt ccatagttgt cccagctgta
     2401 attgaaaatt ccggatgaca cgccggcgca ttttccggag aaaccgatgg acagaccact
     2461 aaccatttgt attggggttc ttcaactaaa aagtcaagat aaaattcaac agcgcctctc
     2521 attgctggat acacagattt aagatattct ctatcgccgc tatattcata tttttcaaat
     2581 aaatgttgac aaagccaagc actgcccatt ggccacattc cccaaaacgc accatctacc
     2641 ggtccgcaaa tacgccaaat atctgtatta tgatgcaata cccacccttc cgcaccatac
     2701 atactttccg cagtttgtct gcctgtttca gagagttctt ctatcatctg aactagcggc
     2761 tcattcattt cgcttaaatt ggttggctca gaaagccaat agttcatctc cgtgtttata
     2821 ttcactgtat atttactatc ccagggtggg taaagctggt tgttccaaat tccctgcaga
     2881 ttagcaggct gtgtaccagg gcgcgatgaa gaaataagca agtaacgacc aaactgaaaa
     2941 cacaatgcaa ccaattgcgg atcgttacct ttagcaaatc gttcaatccg aacatcggtc
     3001 ggatttttta tagaatcagc tactcctaaa ttaatactga ctcgcttaaa atagcgctga
     3061 taatcagcaa tatgattttc taacgcttgt ttgtaatttt ttctcaaggc gtttcgaaga
     3121 tacgactgtg ctttctcgcc agcattttcg ctaatatcgc tgtaattttt aaaactcgat
     3181 gccattgaaa tatagattgt agcaatatct gcattgttta cagttaggct gttttgagat
     3241 gcctcaattg agccgctttc tgtagttact tttacctgta cctgaaattt tacttcgccc
     3301 tttatcgaat cgcaatcgct cgttattcca gacataatca atatatcatt tccctcagtg
     3361 aaaatattta ctgtgccagg atgattcatt gaagcagtaa aattgatttt accgggtttg
     3421 ctcgttgaga ttcggcaaat gataacctga tcaggaaatg aggcaaaaac ttcacgctta
     3481 tacgttactc catcaacttc atagcgggta gtaacaacag cagtttcaat attcagctct
     3541 cgataataat ttgtaaagtt tttgtgttcc ggaaaagaaa ggtaaaggct tcctgcggtt
     3601 tgatagggca taccctgcga atttttacta atgaattttt gatccgctag ttcctgggct
     3661 tctttatatt taccattaaa aatcaattgc cggattttgg gcaatgcttc tttggcttca
     3721 agattatcat tgcgatgtgg cttaccaccc cagatagtgt tttcattgag ctgaatttct
     3781 tcacgcaatg gatcaccaaa aatcatggca cccagacgac cattaccaat tggaagcgct
     3841 tcgacccatt gtttggctgg ctgttgatac catagtttca ttggattatc ggcagaatct
     3901 gagtttctat caggtttttt tgcgcaacta ctaataatca gagcgaaagc caataaaatg
     3961 gaaaattttg gga
//
LOCUS       DAOOMU010000136         3952 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_27622_length_3952_cov_21.380293, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000136 DAOOMU010000000
VERSION     DAOOMU010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27622_length_3952_cov_21.380293"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(339..1082)
                     /locus_tag="PLI55_09470"
     CDS             complement(339..1082)
                     /locus_tag="PLI55_09470"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPB01098.1"
                     /translation="MKIRFSKKLIFIGWIGLTNLLFAQNRIISRFPIGEELTYSAWLN
                     FMKVGYSTITIKAIESVDGFPVYHVESITKTSPFYGKIYRVNDRFNSWIDLDGLFSRR
                     YEKSLREGKYKKDYQAYFDYNEKKAYTSRDTLDIDGRMHDALSVIFWVRAEYLFPGRI
                     IRLNNFDNNKLRLFNVIVSGVETLKVPAGEFQCFVLEPYSDDGKLFKNQNKMTIYLSN
                     DSLRLPVMIDNEANFGRMIFKLENRTVHQ"
     gene            complement(1079..1897)
                     /locus_tag="PLI55_09475"
     CDS             complement(1079..1897)
                     /locus_tag="PLI55_09475"
                     /inference="COORDINATES: protein motif:HMM:NF018047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HPB01099.1"
                     /translation="MSGFEMCCRALHGEELTPEQLKHLCDRDWIRNNARHIYKDKVNI
                     LAEIEVEINGVPQAVVIKLFGWRNRISYWLSPFMRSRAKKSWDASFSLLKCGVKVPKP
                     LTVYTQRRFGFIKCNFLLTEKIENFTLARNWLRDPHAKPSDKRIIVETLAEIVRKMHQ
                     AGMVHSDLTPGNFLINNNNPSEIYLIDLNRLKHKIFLTRSLKMDDVAKLNLCQCGFAE
                     EHSDCLWIIFLEKYDKEEFEKNKLALRLAMRRLKNRRAMKSLRKSSKPKNLPTP"
     gene            complement(1884..2918)
                     /locus_tag="PLI55_09480"
     CDS             complement(1884..2918)
                     /locus_tag="PLI55_09480"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HPB01100.1"
                     /translation="MRYLFFITKPYSISVIRPLYEEIFRGAWGEALIFATPPVKALID
                     FAAPSTDSIKAAIDFQPEIVFVPGNFVHDKIPGLKVQIFHGLCEEKGGHYKITGFFDL
                     YCTSGPLITEKFLQLSKKYGFFKVKETGWPKIDNLLKPYNRNEICQSLQIDPQSKIIL
                     YAPTFSPKFKSSNEILKVLNDIPRSGEQWIIKFHDLMASEDRRCFEKLPQDKFRIYTG
                     YDNTPLLQVADVLISDTSSIVYEFMLLDKPVVTIGAKVRQEKALNIESASDLRAAVER
                     SLENPQEFAENRRRTLAQIHPYIDTLNSQRVLQAVEEFLSGTTPGELKKKPLNLYRKY
                     KVRRQLGVWF"
     gene            complement(2921..>3952)
                     /locus_tag="PLI55_09485"
     CDS             complement(2921..>3952)
                     /locus_tag="PLI55_09485"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HPB01101.1"
                     /translation="VAQAMERDGDFEVFYSTVIDKSRNDYEWSRKIIGDLGGNFITAD
                     NEKERVEKIIKQNFDVVVFGKSRGAEKFCRPSSLAVLLYHGIGVKACYYTDYNPRIDV
                     RYVESQYRLEELRRRNIETELVVTGFPKLDPLIDSSVRDYYSDKYHLAKSKPTILYAP
                     TFYPSSIEVFGESLAEQTKDFNLIVKLHHFSWLMKKYQHQRDLWQTLMQKYEHVRLAP
                     VEEYNIVPLFSCSDILVTEASSTAFEYLATSRPIIIADFVKLRWKHRLFRKNFERQRL
                     DSAIIDELDFAYHLEKKQDLHDVLNRAIHDVEGKRHLLTRKRDQFLGNVDGKASERVV
                     ADLKNRLNK"
BASE COUNT         1159 a          866 c          735 g         1192 t
ORIGIN      
        1 ggctaaagtt caatggtatt aagtttttct ccgattattt agttttatga ataacccaac
       61 accggctcag tggtaagtcc cgcctagcgg gatcataagc cgtatgtcta atgtaaaatt
      121 aaaaaccttg tgtaggttgt gtatgataac caatctaacc ataaattctt gagtgaattt
      181 cagaaaccga gcatcatttt tcatctaccg atatctattg attaagtttg agttttagga
      241 tagagaaaaa aataggttaa agtaagtggt cggaattttt attttaattt cattaaaaat
      301 tatgaccttg aaaatgattg cggttttctg cgaacctgct attggtgaac tgtccggttc
      361 tcgagtttaa aaatcattcg cccaaaattg gcttcgttat caatcatcac cggtaaacgc
      421 aaggagtcat tactaagata aatcgtcatt ttattctgat ttttgaacaa tttcccatca
      481 tcggaatatg gctctaatac aaaacactgg aattcgccag ccggaacttt caatgtttcg
      541 acaccactga cgattacatt aaaaagtcgc aatttattat tgtcgaaatt attaagtcga
      601 ataatccgac caggaaataa atattccgcc cgcacccaaa atattaccga tagagcatca
      661 tgcattcgac cgtcaatatc caacgtgtca cgcgaggtat aggctttttt ctcattataa
      721 tcaaaataag cctgataatc ctttttatat tttccttccc gcaaactttt ttcataacgc
      781 cgcgaaaaga gaccatccag atcaatccaa gaattaaacc gatcgttcac ccgataaatt
      841 ttaccgtaga agggacttgt tttggttatt gattcaacgt gatacaccgg aaatccatct
      901 accgattcga tggctttgat agttatagtc gaataaccaa ccttcataaa atttaaccag
      961 gcggagtaag tcaattcctc accaatcgga aagcggctaa taatacgatt ttgggcaaat
     1021 agcaggttag ttaatccaat ccagccgata aaaattaatt ttttagaaaa ccttattttc
     1081 acggtgtcgg caaatttttc ggttttgatg atttccgtaa agatttcata gctcgacgat
     1141 ttttaagacg acgcatcgcc aaacgcaggg ctaatttatt tttctcaaac tcttctttat
     1201 catatttttc aagaaatatt atccataagc aatcggaatg ttcctcagca aagccacatt
     1261 gacacaaatt caatttggcg acatcatcca tttttaacga ccgagtcaga aatattttat
     1321 gttttagacg gttaagatca attagataaa tttcagacgg attattgtta ttgatgagaa
     1381 aattccctgg cgtcaaatct gaatgcacca taccggcttg gtgcattttc ctgactattt
     1441 cagcaagagt ttcaacgata attcttttat cggacggctt agcgtgtggg tctcttaacc
     1501 aattacgcgc cagggtaaaa ttttctattt tctcggttaa taaaaaattg catttgataa
     1561 atccaaaacg acgctgagta taaaccgtaa gcggcttggg aactttgacc ccacatttca
     1621 gcaaactaaa tgaagcgtcc caggatttct tggcacgtga acgcataaag ggacttaacc
     1681 agtaggaaat ccggttgcgc cagccaaata atttaatcac gactgcctgc gggactccgt
     1741 ttatttccac ttcgatttcc gctaggatat tgaccttatc tttgtaaata tgacgagcgt
     1801 tgttgcgaat ccaatcgcgg tcgcacaaat gcttcagctg ttctggagta agctcttcgc
     1861 catgtaacgc tcgacaacac atttcaaaac cagacaccta actgtctgcg cactttatat
     1921 ttacgataaa gatttaacgg ctttttcttc aattctccgg gagtcgtgcc gctcaaaaat
     1981 tcttctactg cttgtagtac gcgctgacta tttaatgtat caatgtaagg atgaatctgc
     2041 gccagcgttc ggcgccgatt ttcagcgaat tcctgcggat tttccagtga acgctcgacc
     2101 gccgcccgca aatctgacgc tgattcaatg ttaagtgctt tttcttgcct gactttcgcg
     2161 ccgattgtta ccaccggttt atccaacagc ataaattcat acactattga cgaagtatcc
     2221 gatatcaaaa catcggcgac ttgcaataac ggagtattat cgtagccagt atagatccgg
     2281 aatttatcct ggggcaattt ttcaaaacac cggcggtctt cggaagccat tagatcgtga
     2341 aacttaatga tccactgttc gccagagcgg ggtatatcat tgagaacttt tagtatttcg
     2401 tttgaggatt tgaatttcgg gctaaaagtc ggagcgtata gaattatttt gctctgcggg
     2461 tcaatctgta gactttgaca aatttcattt cgattatatg gcttcagtag attgtcaatt
     2521 ttcggccagc cagtttcctt tactttaaaa aaaccatact tttttgacaa ttgcaaaaat
     2581 ttttcagtaa tcaagggacc gctggtacag tagagatcga agaaaccagt aattttataa
     2641 tgtccaccct tttcctcaca cagtccgtga aaaatttgca cctttaaacc gggaatttta
     2701 tcatgaacga aattgcccgg cacgaaaaca atttccggct ggaaatctat cgccgcttta
     2761 atcgaatcag ttgaaggcgc cgcaaaatca atcagggctt tgactggcgg tgtagcaaat
     2821 attaaagcct ccccccacgc tccgcggaaa atttcctcat acaatggccg gataacgcta
     2881 attgaatacg gcttggtaat gaaaaataaa tagcgcattt ttacttattc agacgatttt
     2941 tcaaatcagc gactactcgc tcggatgcct tgccatcaac atttcccaga aactgatccc
     3001 gtttccgggt cagcaaatgt ctcttcccct caacgtcatg tattgcacga ttcagtacat
     3061 cgtgcaaatc ttgcttcttt tcgaggtgat aagcaaaatc gagttcgtca attatcgccg
     3121 agtcaagacg ttgccgttcg aaattttttc gaaaaaggcg gtgcttccag cgcaatttaa
     3181 caaaatcagc gatgattatc gggcggctgg tagcgagata ttcaaaagcc gttgacgacg
     3241 cttctgttac taaaatatca ctgcacgaaa aaagcggcac tatattgtat tcctcgaccg
     3301 gagccaaccg gacatgttca tacttttgca ttagggtttg ccagaggtcg cgctgatgtt
     3361 gatatttttt cattaaccag gaaaaatgat gcaatttgac gatgaggtta aaatccttcg
     3421 tctgctctgc cagcgattcg ccaaaaactt caatcgaact cggataaaag gtcggggcat
     3481 ataaaatcgt cggtttgctt ttcgccaaat gatacttgtc ggaataataa tctctaacag
     3541 agctgtctat cagtgggtcc aatttgggga aaccagttac gaccaactca gtctcgatat
     3601 tccgtcgccg caattcttcc aggcgatatt gactctcgac atagcgcaca tctatccgtg
     3661 gattgtagtc agtataataa caggctttta cccctattcc gtgatatagc aaaactgcca
     3721 gggatgaagg ccggcaaaac ttttccgcac cgcggctttt gccgaaaacc acaacatcga
     3781 aattttgctt aattatcttt tcgacacgct ctttttcatt atcagctgta ataaaatttc
     3841 cgcccaaatc accgataatt tttcgcgacc attcataatc attacgactt ttatcaatta
     3901 ccgttgagta aaacacttca aaatcaccat cacgctccat cgcctgcgcc ac
//
LOCUS       DAOOMU010000137         3881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_28327_length_3881_cov_21.328542, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000137 DAOOMU010000000
VERSION     DAOOMU010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28327_length_3881_cov_21.328542"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1416
                     /locus_tag="PLI55_09490"
     CDS             97..1416
                     /locus_tag="PLI55_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01102.1"
                     /translation="MEQEKRERIYTFDFAKGVAILLIIPLHALICQIGKNDPAFFQPL
                     ITAIPENVIYLLIPAMILSMWGPIFTLITGANIAYGYLRVYSRNPDDSSAYILRRILS
                     AVMLIFVSRAAVFIFKGGLFTNGFFNIFNYKIRYYADTLDSIALTGIIVPVFILLFLD
                     KSKSIKQKNETGKAVSQKDVKRIYIGMTVITVIWFVFTPVVHALTPYVQDFASKHHLR
                     LLMLVWSKLTAGRFRFFPILGFGYAGAVIGTAIHAKSRYKNIRKYSGIFFLVTLVGFL
                     IWILLKGNPMVNIASDDIPIMMQVLNLGTMTFAMVLVLGYLDYCPPEVRRRRIERTLW
                     IRRFSIVSLTIYVMEFFLAEGVYWVFAQFWDNAVALVNEVPIFIWNFAQIMVFIIVIC
                     LIWGLIVKLWEKVDFIFSLEWFMIKAVALFQRDKHARINSHKILYGQ"
     gene            1419..2510
                     /locus_tag="PLI55_09495"
     CDS             1419..2510
                     /locus_tag="PLI55_09495"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB01103.1"
                     /translation="MNKVRVGVVGTSWWADGMYLPALQSHPQAEIVAICGRNRPRATE
                     MAEKYNIPKIYTDYRVMIEQGDLDAIIIGVPDDLHYDIAIRSLEAGLNVLCDKPLALK
                     EQQAYEMWQKAEAAHVQNMVLFTYRWMPFFQYVHDLIKQGYIGQCYQAEFSFLVGHGR
                     KPDYQWRLDSKRSNGALGDLGSHLIYLARWLIGEIAQVDAQLASFAKRLGPDGEPISP
                     ANDSAVLLFEFTNGAQGVIHTSFMTYLADRDMQIYVKLYGSDGSLEINFPSSGSDSTA
                     SIFGAHNSDEKFRRLEIPASYWGDVNPAEPFGIFTRQAVGCRLFIDAILENRPVSPNF
                     FDGYKVQQVIEAAINSDRTGKRVAIDHSR"
     gene            2575..3843
                     /locus_tag="PLI55_09500"
     CDS             2575..3843
                     /locus_tag="PLI55_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPB01104.1"
                     /translation="MLEFSPLLVVSDGHGRVFELEQYQALGISGENVVEIPPDEWIPL
                     PEGSQLMELPGRLPVGKDRRSSEVATLTENGAGKLLAVAAFVAPAYTIFYHAAWEKLP
                     RARRLSLFAYSAVGWFEGCFYVPAVRIDPDIRQDPPNFNQAEIARSAQRQLKLFPHNR
                     LVAHLVNNCALTYGCPAALNYLLNRWEMPLPTARTCNSACLGCISLQRNSGVCSAQHR
                     INFTPTVAEIVEIAVPHLDTAPNPVVSFGQGCEGEPLMNPQLLYQAIKGIRQKTARGT
                     INLNSNASRPDVIIKLIEAGLDSLRVSLNSARPQFYTRYFRPRNYTFDAVLESIRLMK
                     NANRFVSINYFTFPGFSDQPSEIKAFEKILADYNIDLIQWRNLNIDPEWYWEELNPPE
                     EDGLGIRQLIEYYQAEFPRLRHGYFNPSLK"
BASE COUNT          990 a          807 c          911 g         1173 t
ORIGIN      
        1 ccagctatga ggtttaattt gctagtgatt ttcttggttc atgtctggct cggtagtata
       61 ttataaaaaa tatgtagtga ttgcatttta ttaaatatgg aacaggaaaa acgcgaaaga
      121 atatacacct tcgattttgc taagggagta gccattctcc tgattattcc attgcatgcc
      181 ttgatatgtc aaatcggtaa gaacgacccg gcttttttcc agcctttaat aacggccatc
      241 cctgagaatg tgatttacct actgatacca gctatgatat tatcaatgtg gggcccgatt
      301 tttactctga taactggcgc taatatcgcc tacggctatt tacgtgtata tagtcgtaat
      361 ccggatgata gttcagccta tattctgcgg cgaattcttt cagccgtcat gctgattttt
      421 gttagtcgag cggctgtgtt cattttcaaa ggtggtttgt ttacgaatgg ttttttcaat
      481 attttcaatt ataaaattcg ctattatgcc gatacgctgg attcgattgc gctgaccgga
      541 attatcgtac cggtatttat tttacttttt ctggataagt ccaagtcaat aaagcaaaaa
      601 aatgaaaccg gtaaagccgt tagccaaaag gacgtcaaac gtatttatat cggtatgacg
      661 gtaattactg tgatttggtt tgtatttact ccggtagtgc atgctctgac gccctatgtg
      721 caggattttg cctccaaaca tcatttgcga ttattgatgt tggtctggag taaattgaca
      781 gcggggcggt ttcgcttctt cccaatcctg ggttttggat atgccggtgc ggttatcggg
      841 acggctattc atgccaaaag tcgctataaa aatattcgta aatattccgg tatattcttt
      901 ttagtaacgc ttgtgggatt tttgatctgg attttgctta agggaaaccc aatggtcaat
      961 atagcttcgg atgacattcc gattatgatg caagtattga atctgggaac aatgacattt
     1021 gcgatggttc tggtgctggg ttatttagat tattgtccac cggaagttcg ccgacgtcgg
     1081 atcgaacgga ctctgtggat tcggcggttt agtatcgtgt cattgacaat ttacgttatg
     1141 gagttttttc tggctgaggg tgtttactgg gtttttgccc agttctggga taatgccgta
     1201 gcccttgtga atgaagtccc aatatttatc tggaatttcg cccaaattat ggtatttata
     1261 attgtcattt gtctaatctg gggtttgatc gtcaaactct gggagaaagt ggattttatt
     1321 ttttcactgg aatggtttat gattaaagcg gttgctcttt ttcagcgtga caaacatgcc
     1381 cggattaatt cccataaaat tctatatggt caataactat gaataaagtc agagtcggcg
     1441 tcgtcggaac cagctggtgg gcggacggaa tgtacctacc ggctttgcaa agccatccgc
     1501 aggcagagat tgttgcaatc tgtggccgga atcgcccgcg tgccaccgaa atggctgaga
     1561 aatataacat tccaaaaata tatactgatt accgggtaat gatcgaacag ggtgatttgg
     1621 atgcaataat catcggcgtt cctgacgatt tgcactatga tattgcaata cgaagtctcg
     1681 aagccggttt gaacgtgctt tgtgataaac cgctggcgtt aaaggagcaa caggcttacg
     1741 aaatgtggca aaaagcggaa gcggctcacg tccaaaatat ggttttattt acttaccgct
     1801 ggatgccgtt ttttcaatat gtgcatgatt taattaagca gggatatatt ggacaatgtt
     1861 accaggccga gttcagcttt ttagttggtc acggccgtaa gccggattat caatggcgac
     1921 tggatagcaa gcggtctaat ggcgctctcg gcgatctggg atcacatttg atctatttgg
     1981 cgcgctggct gattggtgaa attgcccagg ttgatgcgca attggcttcc ttcgctaaac
     2041 ggctaggacc agatggcgag ccaatatctc cagctaatga ttcggctgtt ttattgtttg
     2101 aatttaccaa cggggcgcaa ggagtaattc acacaagttt tatgacttat ctggctgatc
     2161 gcgatatgca aatttacgtc aagttatatg gttccgatgg tagtctggaa attaatttcc
     2221 caagttcggg tagcgattcc accgcgagca ttttcggtgc gcataattct gacgaaaaat
     2281 tccggagatt agagatacca gcctcgtatt ggggtgatgt gaatccggca gaaccttttg
     2341 gaatttttac ccggcaagcg gtggggtgtc gtctgttcat tgatgctata ttggaaaatc
     2401 ggccggtcag tcccaatttt ttcgatggat ataaagtcca acaggttatc gaagccgcga
     2461 ttaattctga ccgtacggga aaaagagtag ccattgatca ttcgcggtaa attgcctgtt
     2521 aacggacgta acttttcgct caaagaaatc aattaatgat ggtgatgtaa gcaattgctt
     2581 gaattttctc ctttattagt ggtatccgac ggtcacggac gcgttttcga acttgagcaa
     2641 tatcaagcgc tgggcatttc cggtgaaaac gtcgtagaaa tacctcccga tgaatggatt
     2701 ccgctcccgg aaggtagtca actaatggaa ttacccggtc gtctgccggt tggcaaagac
     2761 cgacggagta gtgaagtagc gactctgacc gaaaacggcg ctggaaagtt gctggcggtc
     2821 gcggcttttg tggcaccggc ttacactatt ttttatcatg ccgcttggga aaaattgcca
     2881 cgtgcccggc gactttcact ttttgcttac tccgccgttg gctggtttga gggatgcttt
     2941 tatgtcccgg cggtgcgcat tgatccggac atccgtcagg atccgccgaa cttcaatcag
     3001 gcagaaattg ccagatcagc ccaacgtcag ttaaaacttt ttccccataa ccggttagtg
     3061 gcgcatctcg tcaataattg cgctttaact tacggctgtc cagcggcttt gaattactta
     3121 ttgaatcgct gggaaatgcc gctaccaacg gcgcgcacct gcaactccgc ttgcttgggt
     3181 tgtatttctc tccaacgaaa ttctggcgtt tgcagtgctc agcatcgcat taatttcact
     3241 ccaaccgtgg cagaaatcgt tgaaattgcc gtgccacatc ttgacaccgc gcccaatccg
     3301 gtggtcagct ttggacaggg ttgtgagggc gagcccctga tgaatccgca attactctat
     3361 caagctatca aaggcattcg gcaaaaaacc gccagaggca ctatcaatct aaatagcaat
     3421 gccagtcgtc ccgatgtaat tataaaatta attgaagctg gtttagactc cttgcgggtc
     3481 tcattgaatt cggctcgacc gcaattttac actcgctact ttcgtcccag gaattataca
     3541 tttgatgccg tactggaatc aatccgtcta atgaaaaacg ctaatcgttt tgtctctatc
     3601 aattatttta cttttcctgg ttttagtgac cagccttcgg aaatcaaagc ctttgaaaaa
     3661 atactagctg attataatat agatctgatt cagtggcgca acttgaacat tgacccggaa
     3721 tggtactggg aggaactcaa tccgccggaa gaagacggcc tcggtattcg gcaattaatc
     3781 gaatactatc aagctgaatt tccccgccta cgtcacggtt atttcaatcc ctctttgaag
     3841 taatcaaagc tattccacca ccaaggcacg aaaaataagg c
//
LOCUS       DAOOMU010000138         3789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_29247_length_3789_cov_10.498125, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000138 DAOOMU010000000
VERSION     DAOOMU010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29247_length_3789_cov_10.498125"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PLI55_09505"
     CDS             complement(<1..1441)
                     /locus_tag="PLI55_09505"
                     /inference="COORDINATES: protein motif:HMM:NF033578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HPB01105.1"
                     /translation="MVNYEDPNQTKIVKFTLPFSGHLDPNNRWIRLAEMVPWQELYGK
                     YTKQLCRDFGRMSIKPRVAIGALIIKHFKQLSDEETIQEIQENPYLQYFLGYSSYQFK
                     PGFDPSLFVTIRRRLPARTIEAINEIFIQKVREYEQIEAEHKQSRRQSKACKKGGKTS
                     FPLENSDSSSNKGKLIVDATIAPSNIKYPTDLDLLNDSREISEKLIDKLYLKGLFEHK
                     PRTYRQKARQDFLNVIKKKRKSKKALCKGIRKQLQYLRRNLTHLDQMLAVFKDQPDAL
                     SEREIELLEVIRKIYAQQKEMYINQRHTVKDRIVSIHQPYIRPMVRGKANAEVEFGAK
                     ISVSVVAGYVFLDRLKWDAYNENMDLVDQVNRYRQRFGYYPAVVIADGIYGTRDNRSY
                     LEEHGIRFSGKKLGRPPKEISAIAWEMERLRILEQGERNEVEGKIGTAKTRYGLGKVL
                     ARTKETSENWIAMALLSMNMATALRLTAKP"
     gene            complement(1897..3105)
                     /locus_tag="PLI55_09510"
     CDS             complement(1897..3105)
                     /locus_tag="PLI55_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB01106.1"
                     /translation="MRIFRVVLILSTVFVISSAQWVQMNAPSSSGITYSSFVSTEGAF
                     LVGSNGLGIHQSIDEGLNWTKIANATIENSTHIMTAIDTNIYLGMNTTGVAFSSDNGA
                     TWTNISNGITNKKIMTIAKMGRYLFAAGTSFYRSSDLGATWTESGAGLPTSDKKDIII
                     MDNKVFICAKGSGVYLSTDSGATFTSHNGNLPHTYTKRLLQKGEKVFLWLGNAPYVST
                     DSCATWTTASTGSVDAEPLCVYTDGNIIFNGTFGSGIYYTNNDGGQWLSDNTGLTNLN
                     VRGIIVRGGYVFVGTDGAIWRRPLCEFTTAISKDRRTSTGNFSLFPAYPNPFNPSVTI
                     PYSLAEDGQMSLKVFNLRGELVEVLISNYTLKGTYSVTWQPQNISAGIYLVRLESGNK
                     TNLQKVVFVK"
     gene            3588..>3789
                     /locus_tag="PLI55_09515"
     CDS             3588..>3789
                     /locus_tag="PLI55_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01107.1"
                     /translation="MKPLSGEIDLCFRPPELVTEINPCLIKYKGAPKPEQALMAKSAN
                     GIAVVTGCSHPGIITILTKVKTI"
BASE COUNT         1046 a          882 c          739 g         1122 t
ORIGIN      
        1 ccggtttggc agtaaggcgc aaagcagtcg ccatattcat cgagagtagc gccatggcta
       61 tccagttttc actggtttct ttagtcctgg ccagaacttt tcctaatcca tagcgggtct
      121 tggctgttcc tattttccct tcgacctcat tcctttcacc ttgctccagt attctcaatc
      181 gttccatttc ccaggctatc gcacttatct ccttaggcgg acgacccaat ttcttaccag
      241 agaatcgaat gccatgttcc tccagataac tgcgattatc ccgggttccg tatatgccat
      301 ctgcaatcac aactgccgga taatagccaa acctttgcct gtatctattc acctgatcaa
      361 ccaagtccat gttctcgttg taggcatccc acttcaatcg atctaaaaat acataccccg
      421 ccacaacact tactgatatt ttagctccaa actcaacctc cgcattggct ttccccctga
      481 ccatcggtcg tatataaggc tgatgaatac tcacaatccg atcctttaca gtatgtcgct
      541 gattaatata catctccttc tgctgagcat aaatcttacg aataacctcc agtaattcga
      601 tctcacgctc actaagagca tcaggttgat ctttaaatac cgccaacatc tgatcaagat
      661 gagttaaatt ccgcctcaaa tattgcaact gcttccggat tcccttacaa agcgctttct
      721 tacttttgcg cttcttcttt attacattca gaaaatcctg gcgcgctttc tgacgataag
      781 tcctcggctt atgctcaaat aatcccttca aatacagttt atcaatcaat ttttcactga
      841 tctctcgcga gtcattgagt aaatccaaat cagtcgggta tttgatatta gagggtgcaa
      901 tcgtagcatc tactatcaat ttgcctttat tgctacttga atcactattc tccagcggaa
      961 aagacgtttt tccccctttc ttgcaagcct tggactgtct ccgactctgc ttatgctcgg
     1021 cttctatttg ctcgtattct ctcactttct gaatgaatat ctcattgatg gcttcaatcg
     1081 tccgggctgg caagcgcctc cgaatagtca caaataagga aggatcaaag ccgggcttga
     1141 attgataact cgaatagcct aaaaagtatt gcaaataagg attctcctga atctcctgga
     1201 tcgtctcctc atcacttaat tgcttgaagt gcttaataat caatgcacct atggctactc
     1261 gtggctttat ggacatacgc ccaaaatctc gacacagttg cttagtgtat ttcccataga
     1321 gttcctgcca cggtaccatc tcagctaaac gtatccagcg attgttagga tcaaggtgac
     1381 cagaaaaagg caaggtgaat tttacaatct tagtttgatt ggggtcttca taatttacca
     1441 tttagagctc caaattgatt tgttaaatgc ataggttttg ccaaatatca tcctaaatat
     1501 atacatttac atcaaataaa gcaagcgata aatatctgtt attacaatat attagatact
     1561 ttttcaggaa gccctaatta ttattttgac gtaaacgtgg cttcagtcaa aatcttatta
     1621 aagactcgta tcaaaataaa aagtgtgggc cgttaaatcc atcataatta gttacgccgc
     1681 tggagcggtg taaccaggag atcaaaatga ttggtatgaa aaagggcatt ccgatgtgtc
     1741 gggactgtgt gccccaacag aaacatgcca gtataaataa gaagagtccg gcagctgccg
     1801 gacccctata taatattctc cgtgttcccc gaaaatatcc tccgtggaac tctccgcctg
     1861 tatgactgcg tcagcagtcg ggcagacggg ttgcaattat ttgacgaaga ccactttctg
     1921 caggttggtt ttgttaccgg actccaggcg taccaggtaa atcccggcgc tgatattctg
     1981 cggctgccag gtgaccgagt aagtgccttt aagagtataa ttgctgataa gtacttctac
     2041 taattcaccg cgcagattaa aaaccttcag cgacatctgg ccgtcttctg ccagactgta
     2101 tggaattgtc acgctcggat tgaacggatt cggataggcc ggaaataatg agaaattacc
     2161 cgtcgaagtc ctgcggtctt tgctaatcgc cgtcgtaaat tcgcataacg gtcgccgcca
     2221 gattgctcca tcggtaccaa caaaaacgta tccaccacgg acaattattc cgcgtacatt
     2281 cagattagtc aaaccggtgt tatccgatag ccactgcccg ccgtcgttat tagtataata
     2341 aataccgcta ccaaaggtgc cgttaaatat gatgttgcca tcggtgtaga cgcataatgg
     2401 ttcagcatcg acggaacctg tcgaggcagt cgtccaggtg gcgcaagaat cggtactgac
     2461 gtatggagca ttaccaagcc ataaaaacac cttttcccct ttctgtaaga gtcttttggt
     2521 ataagtgtga ggaaggtttc cgttatgtga tgtaaaggtg gctcccgagt cggtggaaag
     2581 gtaaacaccg gagccttttg cgcaaataaa caccttatta tccataatga ttatgtcttt
     2641 tttatcggaa gtcgggagtc ccgccccgga ttcagtccag gtcgcgccta gatcgcttga
     2701 gcgatagaag gaggtcccgg ctgcgaacaa gtagcggccc attttcgcga ttgtcatgat
     2761 ttttttattg gttattccat tcgaaatatt tgtccaggtc gcaccgttgt ctgaagagaa
     2821 ggcaacacca gtggtattca tacccaggta gatatttgtg tcgatcgctg tcatgatgtg
     2881 ggttgagttt tcgatggttg cattggctat ctttgtccaa ttcaatcctt catcgatact
     2941 ttgatgaata cctaaaccat ttgaaccgac caggaatgca ccctctgtcg acacaaagga
     3001 actataggtt attccacttg aactcggtgc gttcatctgt acccattgtg ccgaactaat
     3061 cacaaaaacc gtgcttaaaa ttaaaacaac acgaaaaatt ctcatacgct ctctcctttt
     3121 tatattaagt cttctctgac ttggtgctta tctaccaagc aacagatcaa tttatctatc
     3181 aattttataa tcgaaaatat aaatatttac cattctgaga attgcagata atcttctcac
     3241 atgacctcac ttggttcaga ttaatgtaaa aatccactaa aactatacgg attaaattta
     3301 taattaattt tcctgatgtg caataatatt ttcaatttaa ccattcaaat ggttggcagt
     3361 gatctgttaa aagtccgtca ggattcgaca tctgacaaaa ctgaagaatc gcttaggtta
     3421 cgtgtttttc tcttgtcact ccggggatgt acacggcgac gagacctgaa ctattcctct
     3481 tattcagaaa tacaagggcg cgccgcgtac ccctacaagt aggaaatatc ctccgtgttc
     3541 ttgtacgttt tgttgtgaat aaaataaata agttagaaat taggaatatg aaaccattga
     3601 gtggagaaat tgatctgtgt tttaggccac cagaacttgt cacggagatt aatccctgcc
     3661 taataaaata taagggagcg ccgaaaccgg aacaagcctt gatggctaaa tccgcgaatg
     3721 ggatcgcggt agtcaccggc tgttctcatc cgggaatcat tacaattctt acgaaagtga
     3781 agacaattt
//
LOCUS       DAOOMU010000139         3731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_29864_length_3731_cov_4.765506, whole genome shotgun sequence.
ACCESSION   DAOOMU010000139 DAOOMU010000000
VERSION     DAOOMU010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29864_length_3731_cov_4.765506"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..525)
                     /locus_tag="PLI55_09520"
     CDS             complement(157..525)
                     /locus_tag="PLI55_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01108.1"
                     /translation="MERILLTLFIAISALLHSCSQWDVLKPVNGQLTVKVLNVEITFD
                     YYEEDITVFNQNWNGARVIISRRESWSEYLDIYDRYVLGQKTDNETAYFEAGDKIKVK
                     IKTTNDEGKDVSRESFFELG"
     gene            complement(541..813)
                     /gene="cas2"
                     /locus_tag="PLI55_09525"
     CDS             complement(541..813)
                     /gene="cas2"
                     /locus_tag="PLI55_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPB01109.1"
                     /translation="MNVLIVYDISDADSRMKISKYLESIGHRVQESVFECKFNHSEMD
                     NVVGILQNLLILPGSIRFYPICRECFSKILYIGKNDKMGECGYIIC"
     gene            complement(815..3634)
                     /gene="cas1"
                     /locus_tag="PLI55_09530"
     CDS             complement(815..3634)
                     /gene="cas1"
                     /locus_tag="PLI55_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HPB01110.1"
                     /translation="MDLTFLDNLSFRQYFMQMRLEEPSNFHYFHGPALNSLLCKALNQ
                     SSLGKNIVIFPVEVGKVDYQAGDLYNFSYTVFFDSGDFGARIRDALIARSEFRDNTGP
                     YFRFSVVDYSEIFTPSMADLQDWLGKCDDVAIRFMTPLRMERQNPKTGKTYFDPGYFE
                     PDRFFQLLYNRIYDLNKLSGKPVPEYQSPDITDFSLVDRNLIWVDAPYTSLKKTFGGI
                     IGTVRLRLNPQDYWRKVLILGQFTHAGNNSAKGFGKYIIDEPEFHSLLTKPAMSVLDR
                     VVRDENLKEAFREVKANSEVPGTDGQSINEFEAGLFDNLKRLKKEIVTGKYRSEDLRG
                     IILPVNEKKVRALAIPSVRDRVFQRAACQILAPAVDQLLEDCSFAYRRGFSREGAAKA
                     IQQAYNEGYRYVLESDIEAFFDNVDWSLLFRKLDTLLFNDPMVPIIHRWIMQTVEYNG
                     QKVQRVRGLPQGSAISPLLANLLLDEFDEQLGDDFRLIRYADDFVILCKTKEDAENAL
                     TQAESVVKQLKLELKPSKTNLTNFDIGFQYLGYIFCKSAVLETKKKSGKEKTKPVMIP
                     VPDIVPKNSWIANVDFRKIKEIHPVLYGNKKEFALIDSSHQDRKAFPVYLSDSEVFAR
                     LSYQTLIITNHDHCGKPGRKIPLSQIKFIVDYGKPSITLPAVIELSRRNIPTYFCRRT
                     GETYCVVPASQTDYNIWQKQLEKSLDKRFVIGFAREIVRAKIHNYAVIARRQQWNDSI
                     LQQFSLMEDRCEQEDDMDSLRGFEGKSAAIYFKTLAEQLPQEFNFNGRQKHPPTDPIN
                     ALLSFGYSVLYHHLATTLMGNNLNPAIGWYHFSHDRYLALACDLQEEFRHIIDSLVIQ
                     MVHRGQVNSSDYFRSEKMKYPFLMKKDFQSKYISLIEDRLLTKFTHLESETEIDLLGL
                     FDYQARQIIELCRGKQEKYRPYRIH"
BASE COUNT         1075 a          859 c          667 g         1130 t
ORIGIN      
        1 aggctcggga ggttcggata taaccggatt atcgcaactt aatatccagg gcgctagagc
       61 tgtagccggt agtaaacgga tcaattgctg gcaaaactgc cgacgagaaa tattttcagc
      121 taaactttcc attttttctc tttcctaaga aatgttttac ccaagttcaa aaaaagattc
      181 tctactaaca tcttttcctt catcattcgt ggtttttatt tttactttaa tcttatcacc
      241 tgcttcgaaa taggcggttt cgttatctgt tttttgcccg agaacatagc gatcatatat
      301 atccagatat tcactccaac tttcacgacg actgattatt acccgggctc cgttccagtt
      361 ctgattaaaa acagtaatat cttcttcata ataatcgaag gtaatctcaa cattcaggac
      421 tttgacagtc aactgaccgt tcactggttt caatacgtcc cattgagaac agctatgtaa
      481 aagagcagaa atggctatga ataatgttaa tagtattctt tccatattaa ctccattaat
      541 ttaacagatt atgtacccac attcacccat tttatcgttc tttccaatat ataaaatctt
      601 ggaaaaacat tcgcgacata taggataaaa ccgaatacta ccgggaagta ttaatagatt
      661 ctgtagtatt ccgacaacat tgtccatctc agaatgatta aacttacatt caaataccga
      721 ttcttgcacc ctatgcccaa tagattctaa atatttggat atcttcatcc tggaatcagc
      781 atcgctaata tcataaacaa tcagaacatt catactaatg aatacgatag gggcgatatt
      841 tttcctgttt accacggcac aattcaatta tctgccgtgc ctggtaatca aacaatccta
      901 ataaatcaat ttccgtttca gattcaagat gtgtgaattt tgtcaacaat cgatcttcaa
      961 tcagtgaaat atatttgctc tgaaaatctt tcttcataag gaacggatat ttcatttttt
     1021 cactcctgaa ataatcagac gagtttacct gacctcggtg gaccatctga attaccagac
     1081 tatcaattat atgcctgaac tcttcctgta aatcacaggc tagtgctaga taccggtcat
     1141 gtgaaaaatg ataccatcct attgccggat tcaggttatt acccatcaat gttgtcgcca
     1201 aatgatggta aagaactgaa tacccaaacg aaagcaatgc gtttatcgga tcggtcggcg
     1261 gatgcttttg acggccattg aaattgaact cctggggtaa ctgctcggca agagttttaa
     1321 aatatatcgc ggcagatttt ccctcaaaac ctctcaaaga atccatatca tcttcttgct
     1381 cgcaccgatc ttccataagc gagaattgct gtaatatgct gtcattccat tgttgtctcc
     1441 tggcaataac agcataatta tggattttag cccgcacgat ttcacgggca aaaccaataa
     1501 caaaccgctt atcgagggat ttctccagtt gtttttgcca gatattataa tcagtctgag
     1561 acgccggaac aacacaatag gtttcgccgg ttcgcctgca aaaatatgtc ggaatatttc
     1621 ggcggctaag ttcaatcact gctggcagag ttatgcttgg tttcccgtaa tcaacaatga
     1681 acttaatttg agataatgga attttccgcc ccggcttccc gcaatgatca tggttggtaa
     1741 ttataagcgt ctgatatgac agtctggcaa aaacctccga atccgataag tacaccggaa
     1801 atgctttcct gtcctgatga ctgctatcaa tcagagcaaa ttccttttta ttaccgtata
     1861 aaacggggtg aatttccttt atttttctaa aatcaacgtt ggctatccag ctatttttag
     1921 gcactatatc cggaaccgga atcatcacag gctttgtttt ttctttccca cttttctttt
     1981 ttgtttccaa tacggctgat ttacagaaga tatatccgag atattgaaat ccaatatcaa
     2041 agtttgtcag gttggttttt gaaggtttta attcaagttt aagttgttta acgaccgact
     2101 cggcttgcgt caaagcattc tctgcatctt ctttggtctt gcataaaatt acaaaatcat
     2161 ccgcgtatct gatcagccga aaatcatcac ctaattgttc atcaaattca tccagtaata
     2221 aatttgccag aagcggagaa attgccgaac cttgaggtag tcccctgact ctttgcacct
     2281 tttgaccatt atactcaacg gtctgcatta tccatctatg tatgatcgga accattgggt
     2341 cgttaaatag aagcgtatcc aattttcgaa ataataagct ccaatccaca ttatcaaaaa
     2401 atgcctctat gtctgattcc agaacatagc gatatccctc attataggct tgctggatcg
     2461 ctttggctgc cccttcccgt gaaaatcctc tccgatatgc aaaggagcag tcttccaata
     2521 attgatctac tgccggagcc aatatctggc aggcggctct ttgaaaaacc cggtctctaa
     2581 ccgaggggat tgccaatgcc cggaccttct tttcattgac tggtaatatt atcccgcgta
     2641 gatcctcaga gcgatattta cctgtaacga tctctttttt cagtcgcttc agattatcga
     2701 acaacccagc ttcaaactca tttatggatt gcccgtctgt accaggcacc tcgctgttgg
     2761 ctttgacttc ccggaatgct tctttcaggt tttcgtcccg gactacccgg tctaagaccg
     2821 acattgccgg ttttgtaagc aaactatgga attccggttc atctatgata tatttcccaa
     2881 aacccttggc gctgttatta ccggcgtgcg tgaattggcc aagaatcagt acctttctcc
     2941 aataatcctg cggattcaag cgcaggcgca ccgttccgat aattccccca aaagtcttct
     3001 tgagagaagt atagggcgca tcaacccaaa tcaaattccg gtcaactaag gaaaaatctg
     3061 ttatatcggg agactggtat tcaggcactg gtttaccgga cagcttattc aaatcataaa
     3121 tacggttata caataactga aagaaccgat caggctcaaa ataaccagga tcgaaatacg
     3181 ttttccccgt cttagggttt tgccgctcca tcctcagtgg agtcataaac cgaatagcta
     3241 catcatcgca ctttcccagc caatcctgca ggtctgccat agatggtgta aaaatctccg
     3301 aatagtctac aaccgaaaac cggaagtagg gaccggtatt atcccggaat tccgagcggg
     3361 cgatcagcgc atcccggatt cttgcaccaa aatctccaga atcaaaaaat actgtatatg
     3421 aaaaattata taaatcgccc gcctggtagt caaccttacc gacttcaacc ggaaaaatca
     3481 ctatgttttt ccccaatgaa ctctgattaa gcgccttgca cagcaaactg ttcaacgccg
     3541 gtccgtgaaa ataatgaaaa tttgacggct cttccagtcg catttgcatg aagtactgtc
     3601 gaaaagacag gttgtctaaa aaagttaaat ccaatattgg ttcctttcgt tgaattctga
     3661 aatttacctt ttagaagatt ggctcaacgc ctcatttccc gcagttgcgg gacttcgctt
     3721 cgctcagctt t
//
LOCUS       DAOOMU010000140         3523 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_32233_length_3523_cov_2.201557, whole genome shotgun sequence.
ACCESSION   DAOOMU010000140 DAOOMU010000000
VERSION     DAOOMU010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32233_length_3523_cov_2.201557"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            268..2151
                     /locus_tag="PLI55_09535"
     CDS             268..2151
                     /locus_tag="PLI55_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPB01111.1"
                     /translation="MANYIKRGTFVLSNTYWNLLIAFYCFWIIVNYIEKKYQRLERVN
                     FLGAIALSARTNLYILFLISFVTILMRLPGFSRMHVLSCCAILFVTESTLFGINNHKL
                     RSQSSILITGGYRPALFKISWVRFFLDLFLLLLSFTIVNEIRRSSFHFSDDYLLIAYI
                     ITGLWFITSIFTQKFQKNGYQKNIWYYISPFIKSLILIIFGLSISLFLLKYYHLSHFE
                     LFGTLLIFGLLELTIFSTYYLIGKSESRNGDIESIEEIQKITKQKLLNIDENDQLDEY
                     CVSPSLEYLLESDKRELYEFINSNIDVVNIPNNSLTVLDTRTDINIVNLKSESISLLI
                     NLHKLNDIRWINRYLLTLYEKVSIGGYIVGNLQTNEQYKEQFFCKMPSYLARVLFIPN
                     FIFRRAIPKLPFFKQIYFSLTDGKNRALSKAEVFGRLHFCGYSVIAEKEINKHLWFIS
                     RKVKTISADQNPSYSPLIKLPRVGLNGNTIYLYKIRTMHPYSEYLQEYIYKNNLLDEK
                     GKIKNDFRVTGWGKQLRKLWIDELPQIINWWRGDVSLVGVRALSHHFFDLYPEDMQKL
                     RVQFKPGLVPPYYADMPRTFDEIVESERNYLLRKKEKPFTTDVIYFFKAFYNILFKHA
                     RSR"
     gene            2351..>3523
                     /locus_tag="PLI55_09540"
     CDS             2351..>3523
                     /locus_tag="PLI55_09540"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HPB01112.1"
                     /translation="MKKHLKFIFLLLSLTSGLLAKSTPLIYLDNELIYHYLDYQINTG
                     KNIPQFALQQPFNISLLQLPDTSDKINQYFLSRLIEFYGVKNTAVGIEHQGFIKKSDS
                     WSYSYKFDFDFHYNSDLISIGNRTRVDRNYNNDPYYAGDLSEASHWIYGRVNDAYLSL
                     NFQRIGIFIGKTDRNWGPIGENSLILSGNPYSYDHIMFNYTGRRMLLTAFFSQLENAR
                     GTEYNPDDSTCIFFQKVNRYLSGHRLDISISERFQIGLTEMAIYGGENRNVELAYLNP
                     LTFYYGVQRNDNKGMSGLWALDIMFKPVQRVTLYGQFLIDDIIVNNDPGVNDRERYPD
                     RLGLLTSLRTGDLLFEGLNIDVSFVKIWNRTYQSLRSWENYHYRGLSLGYPIVSCEE"
BASE COUNT         1112 a          527 c          604 g         1280 t
ORIGIN      
        1 tatgatatcc ttgcgagtct tattggtatt gccattggct tgagtttgtt tcagttttat
       61 catattttca gtttaaaaca taagcgaatt gatctatatc cagagtgatt cagggatatc
      121 tttccttaaa ttaaaaacta agacttcaag tcttcgtatt aataatttta ttcattaatg
      181 ccaatgaact attctaataa taaattagac aggacaaaaa aatggattaa gctagtagat
      241 tttctactgg ttggattttc gttttttatg gctaattata ttaagagagg aacttttgta
      301 ctatcgaata cttattggaa tcttttgatt gctttttatt gtttctggat aatagttaat
      361 tacatagaga aaaaatacca gcgcctggaa cgtgttaatt ttcttggtgc tatagcgcta
      421 tctgctagaa ctaatctcta tattttattt ctgatttcct ttgttactat cttaatgcga
      481 ttacccggtt tctcacgtat gcatgttcta agttgttgtg cgattctctt tgttactgaa
      541 agtactctat ttggtataaa taatcacaaa ttaagaagcc aatcttcaat tttgattacg
      601 ggtggatatc gccccgcatt attcaagatt tcctgggttc gatttttttt agacctattt
      661 ttattattat taagttttac tattgtaaat gaaataagac gcagtagttt tcatttttcc
      721 gatgattatt tgttgattgc ctatatcata actggtcttt ggtttattac ttcgattttt
      781 acccaaaaat ttcaaaagaa cggttatcag aaaaacattt ggtattatat ttcgcctttt
      841 atcaaatctc taatattaat tattttcgga ttgagtatat cattatttct attgaaatat
      901 tatcatcttt cgcattttga actgtttggt acattgctta tttttggatt actggaattg
      961 actatatttt cgacttatta tcttataggt aaaagtgaat ctcgaaatgg tgatattgaa
     1021 tctattgagg aaatccaaaa aattaccaaa caaaagttgc taaatataga tgaaaatgac
     1081 cagttagatg agtattgcgt cagtccatca ctagagtatc ttctagaaag tgataagcgt
     1141 gagttatatg agtttatcaa tagtaatatt gatgttgtga atataccaaa taattcattg
     1201 actgtattgg atacaaggac tgatattaat atcgtgaatt tgaaatctga atctatttca
     1261 ttgcttatta acctgcataa attgaatgat atccgctgga ttaatcgcta tttattgacc
     1321 ttgtatgaga aagtatctat cggaggctat atagttggta atctccaaac taatgaacag
     1381 tataaagagc agtttttttg taaaatgcct tcttacctgg cgcgagtttt atttataccg
     1441 aattttattt tcagacgagc tatccccaaa ttaccgtttt ttaaacagat atacttttct
     1501 ttaaccgacg gcaaaaatcg tgcactatca aaagccgaag tttttggtcg tctgcatttt
     1561 tgtggttact cagttattgc tgaaaaggag ataaataaac atctttggtt tatttcccgc
     1621 aaagtcaaga ccatatcagc tgatcaaaat cctagttatt cgcctttgat caaattgcca
     1681 cgggttggac tgaatggtaa cactatttat ttatataaaa ttaggacgat gcatccttat
     1741 tccgaatatc ttcaagaata tatttataaa aataatttgc tagatgaaaa aggtaaaatt
     1801 aaaaatgatt ttcgggttac cggctggggc aaacaactca ggaaactatg gattgacgaa
     1861 ttgcctcaga tcatcaattg gtggcgcggt gatgttagtt tggtaggtgt tcgagcgctc
     1921 agtcaccatt tttttgattt atatccggag gatatgcaaa aattacgtgt acaatttaag
     1981 ccaggtcttg taccccctta ttatgctgat atgcccagga cttttgatga aatcgttgaa
     2041 tccgaaagaa attatctctt gcgtaagaaa gaaaagccct tcacaacaga tgtaatctac
     2101 tttttcaaag cgttttataa tattttattt aaacatgctc ggagtagata atcaaagttg
     2161 ttattttgaa cgaagtgaag aatttccaag atttcttcgg tttattttta ttaagattaa
     2221 attgtaattt acctataatt ttagccgaat gacttaacga agaatcccca taatcgagcc
     2281 taataattca aactttttca aatatttcag tcatttcatt ttcttcaaaa tgattctttt
     2341 aagaaaaata atgaaaaaac acttaaaatt tatattctta ttacttagtt taaccagcgg
     2401 actattagct aaatcaacac ctttgattta cctcgataat gagctgattt atcactattt
     2461 agattatcag atcaataccg gtaaaaatat tccacaattt gcattgcaac agccctttaa
     2521 tatttcattg ttacaattac cagatacatc tgataaaata aaccaatatt ttttatcaag
     2581 attaatcgaa ttctacggtg tgaaaaatac agcagttgga atcgagcacc aaggatttat
     2641 caaaaaatcg gattcctgga gttactcata taagtttgat tttgattttc attataattc
     2701 agatttaatt agtataggga atcggactcg agttgatcgg aattataata acgacccata
     2761 ttacgcagga gacttatccg aagcaagcca ttggatttac ggtcgcgtca atgacgccta
     2821 tttaagccta aattttcaaa ggatcggaat ttttataggt aaaacagacc gtaattgggg
     2881 tccgattgga gaaaacagtt taatattatc cggtaacccc tattcctatg atcatattat
     2941 gtttaattat actgggagac ggatgctgtt aaccgcattt ttttctcagc ttgaaaatgc
     3001 gagaggtact gaatataatc cagatgattc gacttgcatt ttttttcaaa aagtaaatcg
     3061 ttatctttcc ggccatcgtc tggatatttc catttctgaa agatttcaaa ttggtttgac
     3121 tgaaatggca atctatggtg gtgaaaatcg taatgttgaa ttggcttatc ttaatccgct
     3181 gactttttac tacggagttc agcgtaacga taataagggt atgagtggtc tttgggcttt
     3241 ggacattatg tttaaacctg ttcagcgagt tactttatat ggacagtttt taatcgatga
     3301 tattattgtc aataatgatc caggagtaaa tgatcgagag cgatatcccg atagattggg
     3361 attattgact tcgttaagaa caggcgattt gctatttgaa ggattaaata ttgatgttag
     3421 ttttgtgaaa atttggaata gaacctacca gtcattaaga agttgggaaa attatcatta
     3481 tagaggatta agtcttggtt acccgatcgt aagttgtgaa gaa
//
LOCUS       DAOOMU010000141         3468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_32894_length_3468_cov_16.650747, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000141 DAOOMU010000000
VERSION     DAOOMU010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32894_length_3468_cov_16.650747"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3468
                     /locus_tag="PLI55_09545"
     CDS             <1..>3468
                     /locus_tag="PLI55_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB01113.1"
                     /translation="FITKEKNEVPVLLSATARKDEEDNIFGYFMSMLDITKRRQYEQA
                     LKESERRFSAFMETLPHTAFIKTGDHKYLYINNAMKKRFGIEEWVGKTTFDLFPKEIA
                     EKLVKADNISLVKGYHIEESTVPDKSGNECIWETYTFRIERENQEPLIGGFSMDITEK
                     KEAEKKLRENEEFLKSIIESIQDGISILKPDLTINFTNDVMKKWYAENLPLEGKKCYE
                     CYHNANAPCDPCPTLRCIQSGETEWNIVPGLPDSPIKWIELFSYPIKDPDTGKVTGVA
                     EFVRDITSKRQAEEALRQSENKYRLLFQTSPDLIAQIDKEGRFITANPAMADSFGISA
                     EELIGKTFFEVMPPDIAQHRLEMVRKALKEDRIQNFEDERDGKYYYNLFVPVKISEQE
                     EATVQVIARDITESKQMQNQLLESERKYRDLVENMAEGLITIDEQKIVTLVNSKICHM
                     LEFKPEEMVGKNIFDFFDSKNQKILKQELVKRQEGTFSRFEITFTTKSGKQIPVLISG
                     APNFDEKGRFRGSYANVLDFTERKQAEEALRQSEEKYRLITETTSDLISLHTFDLNAS
                     YTYVSPSIKRIAGYEPEELLGKSAFDFIHPDDRKDLLKILETYIGYMFKNLMSGKKLE
                     FTETIEYRFKHKSGKWIYLQSVGNTLGKQLLFVSRDVTQRVQMEASLRENEERYRSVV
                     ENSAMGIFVIDDNYTFEFVNQRLGQILGYNESEIIGHKFMEFLAPESIELVQDRYQHR
                     QKGEAVPTNYEFEVIRKDGERRQVEIYSNVVKDTDGKNHTIAQILDITERVKTEAERD
                     SLASQLHQAQKMEAIGRLAGGIAHDFNNILTIILGYSQMALMNLQPDQKFYSQFQKIN
                     DAARRSANMIGKMLAFSRQQNAEPQRLNLNTHLESTKGLLEKMVREDIKMSFIPGSRL
                     HDIYLDPTQFDQIIMNLVVNARDAMPKGGTLIIETANFTCDSEYSLKHHGFQPGNYVM
                     ISVSDTGCGMDKETLEHIFEPFFTTKGEGEGTGLGLATVYGIIKQNNGFINVYSEPGK
                     GATFKIYFPVYEGQDEKPYQAEDLENIMGQETILLAEDDTDVRTFTRNALQQLGYQVI
                     DFGEPIEALEYFKKHSDEIDLLFTDLVMPGMNGQELHRQIEQIKPGIKVLFTSGYTSN
                     IIAVQGIIK"
BASE COUNT         1157 a          655 c          738 g          918 t
ORIGIN      
        1 tttcattacc aaagaaaaaa atgaagttcc ggttcttctc tccgccaccg ccagaaaaga
       61 tgaagaagac aatattttcg gctattttat gtcaatgctg gatatcacca agcgcaggca
      121 atacgaacaa gccttaaaag aaagtgaacg acgattttcc gcttttatgg aaacactccc
      181 ccatacggct tttatcaaaa ccggcgatca caaatatctt tatatcaata atgcaatgaa
      241 gaagcgcttt ggaattgaag agtgggttgg taaaactact tttgatcttt tcccaaagga
      301 aatagctgaa aagttggtta aggctgataa tatttcactt gtcaaaggtt atcacataga
      361 agagagtacc gtccccgata aaagtggcaa tgaatgtatc tgggaaacct atacttttcg
      421 aatcgagagg gaaaatcaag agccattaat cggcggtttt tcaatggata ttaccgagaa
      481 gaaggaagct gaaaagaaat tacgggaaaa cgaagaattt ctaaaaagta ttatagaaag
      541 tatccaagat ggcatcagca ttctaaaacc ggatttgacg atcaatttta ccaatgatgt
      601 gatgaaaaaa tggtatgccg agaacctgcc acttgaaggg aaaaaatgct acgaatgtta
      661 tcacaatgct aacgctccct gcgatccttg tccaactctt cgctgtattc agtccggcga
      721 aaccgagtgg aatattgttc cagggctccc cgactctcct atcaagtgga ttgaattatt
      781 cagctatcct ataaaagatc cggatacagg caaagtaacc ggtgtggctg aatttgtgcg
      841 tgatattact agtaaaaggc aggcggaaga agctctacgt cagtccgaaa acaaatatcg
      901 cttgttattt cagacttctc cggacttaat tgctcaaatt gataaagaag gtaggtttat
      961 taccgcaaat ccagcgatgg cagatagttt cggcatctca gcagaagaac tgattggtaa
     1021 aacatttttt gaagtaatgc ctccggatat tgctcagcat cgtctcgaga tggtacgaaa
     1081 ggctttaaaa gaagacagaa tccaaaattt tgaagatgaa agagatggaa aatattatta
     1141 taatttattc gtcccagtca aaatttctga acaggaagaa gccaccgtcc aggtaatcgc
     1201 gagagacatt actgagagca aacaaatgca gaaccagtta ttagaatcgg agcgtaaata
     1261 tagagatctg gtcgagaata tggccgaggg tttaatcact attgacgagc aaaaaatcgt
     1321 gaccttagtg aacagcaaga tttgtcatat gttagaattt aaacctgagg aaatggtcgg
     1381 aaaaaatatt ttcgatttct ttgattccaa gaatcaaaag attctaaaac aagaattggt
     1441 taaaaggcaa gaaggcacat tttctcgatt tgaaatcacc tttactacaa aatctggaaa
     1501 acaaatacca gtgcttattt cgggagctcc taattttgat gaaaaaggta gatttcgtgg
     1561 ctcttacgcc aatgtattag actttaccga acgcaaacag gccgaagaag ccctccggca
     1621 gtcagaagaa aaataccgct tgattacaga aactacgagt gatcttattt ccttacatac
     1681 tttcgatttg aatgcttcat atacctatgt aagtccttct attaaaagga ttgccggtta
     1741 cgaaccggaa gaactactgg gcaaatcggc attcgatttt atccatccgg atgatcgaaa
     1801 ggatttactt aaaatattgg agacttatat cggttatatg ttcaaaaatc tgatgtccgg
     1861 aaaaaaatta gaattcacag agacaatcga ataccgattc aaacataaat ctggaaaatg
     1921 gatttatctt cagagtgttg gtaataccct cggcaaacaa ctcctattcg tatcaagaga
     1981 cgtcactcag cgcgtacaga tggaagcctc tttacgtgag aatgaagaaa gatatcgctc
     2041 agtggtggaa aacagcgcta tgggaatttt tgttattgac gataactata cttttgagtt
     2101 tgtcaatcaa cggctcggtc aaattctagg gtacaatgaa tccgagatta tcggacataa
     2161 gtttatggaa tttttagcac cggagagtat cgaactggta caagatcgtt atcagcatcg
     2221 ccagaaaggg gaagcggttc cgacaaatta tgaatttgaa gtaattcgca aggacggcga
     2281 aagacgccag gttgagattt attcgaatgt ggtaaaagat actgatggaa aaaaccacac
     2341 tatcgcccag attctcgaca ttacggaacg ggtaaaaacc gaagccgaac gggacagtct
     2401 ggcatctcaa ctccatcaag ctcagaaaat ggaggcaatt ggacggctgg ctggtggtat
     2461 cgcccatgat tttaataata tactaactat tattctgggc tactcgcaga tggcgttaat
     2521 gaatttacag ccagatcaaa aattctactc ccaatttcaa aaaattaatg atgccgctcg
     2581 tcgttcagcg aatatgattg gcaaaatgct ggcattctcg cgccagcaaa atgccgaacc
     2641 tcaaagacta aatctgaata ctcatctgga atctaccaaa ggtctgttgg aaaaaatggt
     2701 gcgcgaagac attaaaatga gttttattcc cggatctagg cttcatgaca tttatctgga
     2761 tccaacccaa ttcgatcaaa ttattatgaa tctggtcgtc aatgcccgtg atgctatgcc
     2821 aaaaggggga actctaatta tcgaaacggc taatttcacc tgtgattccg aatattccct
     2881 gaagcatcac ggctttcaac cgggcaatta tgtcatgatt tctgtcagtg ataccggttg
     2941 cggaatggat aaagaaaccc tggaacatat attcgaaccg ttttttacga ccaaaggaga
     3001 aggtgaggga accggccttg ggcttgctac ggtttacgga attattaaac aaaacaatgg
     3061 atttattaac gtatatagcg aaccaggtaa aggtgccacg tttaagatct atttccccgt
     3121 ttatgaaggt caagacgaaa aaccttatca agcggaagat ctggaaaata taatgggaca
     3181 agagacaatc ctgctggcgg aggatgatac cgatgttcgc acttttaccc gaaatgcact
     3241 gcaacaatta gggtatcaag ttatagattt tggcgagccg attgaagcgc tggaatactt
     3301 taaaaaacac agtgacgaaa ttgatttatt gttcaccgat ttggtcatgc cgggaatgaa
     3361 tgggcaggaa ctgcatcgcc aaattgaaca gattaagccc ggcattaaag tgctctttac
     3421 atccggatac acctctaata taattgccgt tcagggtatt attaaaag
//
LOCUS       DAOOMU010000142         3424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_33430_length_3424_cov_12.718314, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000142 DAOOMU010000000
VERSION     DAOOMU010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33430_length_3424_cov_12.718314"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1718)
                     /locus_tag="PLI55_09550"
     CDS             complement(<1..>1718)
                     /locus_tag="PLI55_09550"
                     /inference="COORDINATES: protein motif:HMM:NF023128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB01114.1"
                     /translation="KDKQVFAWAMYDWANSAFATTVMAGFFPVFFKGYWSAGTDVNVS
                     TAMLGYANSIGSLIVALISPILGAIADRSSSKKRFLIFFAYLGVLTTTCLFMVDYGQW
                     LWAVIFYVAGSLGFAGANTFYDSLLPEVAPPHKIDYVSAKGYALGYLGGGLLFLINVL
                     WYLSPATFGFPVEQQANVYQALTQNATVIQIEPGKDFSIPKKHSIGKAVVTSEIKLPI
                     TIVDYHSDDSVWISVPVPPEVDQDLIVSAISFGQFKSGEIVAKNHSKNEYLIGNLNQK
                     LSPGDSAVIIIRNPITYQSFRDQRIEGVEGLSNHCGRITITTDYLTPAQENLPIRVSF
                     LSVGLWWGIFTIPLILYVKEKRREPRPNRRSTYIRQGFKQLSQTFQQVRHLKVVMIFL
                     VAYWLYIDGVDTIIRMAVDYGLSIGFPSSSLIVALLIVQFVGFPAALAFGKLAEKWNP
                     KKVIFATIAVYLGVTAYSVLMKDVKEFYILAIVIGCVQGGIQALSRSFYLRLIPKEQS
                     AEFYGFFNMIGKFSVIFGPFLIATVGLLARRAGFSADLASRIGIGSIGILFLVGAILL
                     CFVNEE"
     assembly_gap    1719..1818
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1819..2813)
                     /locus_tag="PLI55_09555"
     CDS             complement(<1819..2813)
                     /locus_tag="PLI55_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPB01115.1"
                     /translation="MKRDLLLIVALISLISIEGTMAKERTGWGWGGVPAINYNADEGF
                     GYGMIFDIFNYAKGGYSPYYFKIDNQIFFTTGGKQDHTIFFDSPYILGNGFRISVRTR
                     YKKEKFQPYYGLGNDSEFDPSYIEVDEDGNSLDSLHGKHYYTFNSEQIIFYTNILKAL
                     KYREDGRPLISVLGGLGFYQLDCGANDNGGIPTKFEEDLNAGILKKHDTDKSFNDYLK
                     FGLVYDSRDNEPAPNRGVWTDIVGEWYTSLIGSDNDFLRLTLTDRRYFTLRKNLVYAN
                     RIIVENIFGEAPLLLYYPLGGSFRIDEGVGGYRSIRGAYKNRYVGSAKFIMNMELR"
     gene            complement(2885..>3424)
                     /locus_tag="PLI55_09560"
     CDS             complement(2885..>3424)
                     /locus_tag="PLI55_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPB01116.1"
                     /translation="ISPNENLNIGIRYETITKMELENDTKKDDTGLFPDSAKYGADIP
                     SQLAVGVSYNMGKIKLMMDFNYYNNGAVDWDGDEKNFDNTTEFGLGAEYALTEKLKVS
                     LGGQYSMMGAKPESQSDMDFNLDAFTIGGGVEYQISPKVKLNVGALDAFYIEAENASG
                     IEKYNQTNYLVAFGVQVKL"
BASE COUNT         1045 a          800 c          590 g          889 t
ORIGIN      
        1 ttttcttcat tgacgaaaca gagtaaaatt gccccaacca gaaataatat cccaatcgaa
       61 ccaattccaa tccgtgaagc caaatcagcc gaaaatccgg cgcggcgagc caatagaccc
      121 acagtcgcga ttaaaaacgg cccaaaaatt accgagaact taccaatcat attgaaaaaa
      181 ccatagaatt cggcgctctg ttctttgggt attaaccgta aataaaacga acgactaaga
      241 gcctgaatac cgccctgaac acatccgatt acaattgcca gaatataaaa ctctttcaca
      301 tctttcatta aaacgctata ggctgttact cccagataga cggcaattgt cgcaaaaatt
      361 actttcttgg gattccattt ttcagctaat ttaccaaacg ccagcgccgc cggaaaacca
      421 acaaactgaa caatcaacaa agccacaatt aatgaactcg acggaaatcc aattgacaat
      481 ccataatcta ccgccattcg gataatcgta tcaacaccgt caatatataa ccagtaggct
      541 actaaaaaaa tcattacaac tttgagatgg cgcacttgct ggaaggtttg gctcagttgt
      601 ttgaatccct gacggatgta cgtagaacgt ctattcggtc tcggctcccg gcgtttttct
      661 ttgacataaa gaattagcgg aatagtgaaa attccccacc agagccctac cgataaaaaa
      721 ctcacccgaa tcggcagatt ttcttgggct ggagtaagat aatcggtggt aatcgtaatc
      781 cgcccacagt gattggaaag tccctccact ccctcaatcc gctgatcgcg aaatgattga
      841 taagtaatcg gatttctaat gataattaca gcggaatctc ccggactcaa tttttggttc
      901 agattgccaa tcaaatattc attcttacta tgatttttag cgacgatctc acccgattta
      961 aattgaccga aactaatcgc agacactatc aagtcctgat caacttcagg tggaacaggc
     1021 acactgatcc agactgaatc atcagaatga taatcaacta tcgtgatggg taatttaatt
     1081 tcactggtga caaccgcttt accgatggaa tgcttcttgg gaatcgaaaa atccttacct
     1141 ggttcgatct ggataaccgt agcattttga gtgagagctt gatagacatt tgcctgttgc
     1201 tcaaccggaa atccaaaagt cgccggacta agataccaca aaacattaat gagaaataat
     1261 aatccacctc ccagataacc cagcgcatag cctttggcag acacataatc aattttatga
     1321 ggcggggcaa cctccggcaa taaactatcg taaaaggtat tcgcaccggc aaaacccagc
     1381 gacccagcta catagaaaat aaccgcccac aaccattgcc catagtcaac cataaaaaga
     1441 caggtcgtcg tcagaacacc caaataagcg aaaaatatca aaaagcgttt cttcgatgaa
     1501 ctccgatcgg caattgcgcc cagaattggc gatattagag ctacaattag cgaaccaatc
     1561 gagttagcgt atcctaacat tgcggtactg acatttacat ccgtaccggc gctccaataa
     1621 cctttgaaaa acaccgggaa gaacccagcc attaccgtcg tggcaaaagc cgaattagcc
     1681 caatcataca ttgcccaggc aaaaacttgc ttgtctttnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnncg caattccata ttcataataa atttggctga gccaacatag
     1861 cggtttttat aagcgccgcg aatcgaacga taaccaccaa cgccttcatc aatgcggaat
     1921 gaaccaccta atggataata caacagaagt ggcgcctcgc caaatatatt ctcaactata
     1981 attcgattcg catataccag attcttacga agtgtaaaat aacggcgatc agttagcgtc
     2041 aatcttaaaa aatcattatc actaccaatt aaactggtgt accattcgcc cacaatatcc
     2101 gtccagacac cgcgattcgg cgccggttca ttatcccgcg aatcgtaaac caacccgaat
     2161 ttgaggtaat cgttaaacga cttatcagta tcatgttttt ttagtattcc agcgttaagg
     2221 tcttcttcaa atttggtggg aattccgccg ttatcatttg cgccacaatc tagctgataa
     2281 aagcccagtc cacccaatac tgaaatgagc ggccgcccat cctcccgata tttcaaagct
     2341 ttcaaaatat tagtataaaa aatgatttgc tcggaattaa aagtatagta atgcttgcca
     2401 tgcaaggaat caagactatt gccatcctca tcaacttcga tataactggg atcgaattcc
     2461 gaatcgttgc ccaatccata atagggctgg aatttttctt ttttataacg ggttcggacg
     2521 cttattctga aaccattacc caaaatatag ggtgaatcga aaaagatggt gtggtcttgt
     2581 ttcccaccgg tcgtgaagaa aatttggtta tcaattttaa aatagtaagg cgaatagcca
     2641 cctttggcat aattgaaaat atcaaagatc attccgtaac caaatccctc atcggcgttg
     2701 taattgatag ccggaacgcc accccaaccc caaccggtgc gttccttagc cattgtccct
     2761 tcgatggaaa ttagcgatat tagtgctact attaataata agtctctctt cattttaacc
     2821 tcaaaatata aataggagaa gccgtaacga cttctcctat ttatcattta atcctggcat
     2881 aattttacag ctttacctga acgccgaaag ctacgagata attagtctga ttgtactttt
     2941 caattccgct ggcattctca gcctcaatat agaaggcatc cagtgcaccg acgttgagct
     3001 ttactttcgg tgaaatttga tactcaacgc caccgccaat tgtaaacgca tcgagattga
     3061 agtccatatc ggactgcgat tccggttttg cacccatcat actatattgt ccaccgagac
     3121 tgacttttaa tttttcagtt aaagcatatt cagcacccaa cccgaattcc gtcgtattgt
     3181 cgaagttttt ctcatcgcca tcccagtcaa cggcgccgtt attgtaataa ttaaaatcca
     3241 tcattagttt aatctttccc atattataag atacgccaac ggctagctga gaaggtatat
     3301 cggctccata tttagcgcta tcagggaaaa gaccggtatc gtcttttttc gtatcatttt
     3361 ccagttccat cttagtgatg gtctcataac gaataccgat attcaagttt tcattcgggc
     3421 tgat
//
LOCUS       DAOOMU010000143         3399 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_33782_length_3399_cov_5.212022, whole genome shotgun sequence.
ACCESSION   DAOOMU010000143 DAOOMU010000000
VERSION     DAOOMU010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33782_length_3399_cov_5.212022"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3399
                     /locus_tag="PLI55_09565"
     CDS             <1..>3399
                     /locus_tag="PLI55_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01117.1"
                     /translation="IIIQTNNTSMGDIVTGTTIFDQLQLEVGGGSNLTSQDVTVTFSV
                     DMSSFNGEFAAVSLQGGTLPLNWYPGSTPMNDSDGDGIYTLDILFPAGTGKIVEYKYT
                     ALNTSLTDTAWFWENFAGNRSFEIDDSSPIQRLRLDSWISGGVTTAALSYSFDRTTSD
                     PLISTWATGSGQINLSDNISIIKEGIAALQADWTINHGDDWGGNTSIEYQKTAPFGEY
                     LDVSGADYLTLWVYNMTSASQSGKTSFWFNLLEAGGDAVYDGTYNSAEHWLYRISGVF
                     DQGPGWNQIRMPLIDRGAENPNDQGFSAPGWNGVANNGILDKDRIVGWHIIIQTENTA
                     IGDFVSGTTLFDQLQLETGEIVGEFSVTFQADLTDFIANGWFNPANPADSIVVNGDFN
                     GWDSHQKMLPSESNPNIYLFTTVVTGNPGDIVGWKFRIFPDSKWTNNGWESIENRELI
                     LRENNIVLESIKPIIYYRGNPITQDVTVTFTVDMSNFDGSFSAVSLQGNALPLNWSPG
                     SLPMDDSDKDGIYTVAVFFPSGTGKTIEYKYTALTDTAWVWESFVGNRSFEIDDSSPD
                     QILAVDIFNGYGTALNASVYLNGQKARLRVTDSAPINPAANPSAYVITGKAITVETWV
                     FPLNLPENGNCAVLVSRPYWNTDPWYAYELRINNFGPTDEPRFDFVVSDGNPGGTHNG
                     VISSNPITIGEWTHVVGTYDGSNLHLYVNGELVGEASFTANIGQGDAGFYLGGTWSNY
                     FDGVIDEVRLWNVCRSQADIQNTMNQQLVGDEAGLVGYWPLDEEIEVNGSYPVCADIT
                     TNHNDLLMQNGARIISASAPLVEPEIAPEFQIQELYGIANINFSYQILCSGWHKPTFT
                     LVNGPQGLILNNGWVYWTPTTAGHYSLVLEARNSAGVTQGIYHIWVDSILVSTDTHNN
                     NNVLLNVYNNGFIGNTLPDLDGNGFQYNGTNGLFIGSLIIAQSANQVAGKIYNNEFGT
                     LEPVHSVTSMLTGFDQAFESRFNDTRKLNPIGVEVVQRTHSKSTNPDQDYVLVEYEIY
                     NKSGRQLTDLYIGLAMDWDVGNDATHNFSGYDAEKKLSYAFLNNETPYFGITALTGDV
                     TGYKTWLANSGEDENSDEFLYLAMTQFSDLPSSAGDIRNVLGTG"
BASE COUNT          952 a          664 c          783 g         1000 t
ORIGIN      
        1 aaataatcat tcaaactaac aacacttcta tgggtgatat tgttactggg acgaccattt
       61 ttgaccagtt gcagttagaa gtcgggggtg gtagtaatct tacctctcaa gatgttacag
      121 ttaccttttc agtggatatg tcgagtttca atggtgagtt cgctgctgtt tctctacagg
      181 gtgggacatt acctttaaac tggtatccgg gttcgactcc gatgaacgat tcagatggcg
      241 atggcattta tactttggat attctatttc cagctggaac cggtaaaata gtcgagtata
      301 aatatacggc acttaataca tcgcttaccg atacagcctg gttctgggaa aattttgccg
      361 gcaatcgcag ttttgagatt gacgacagtt caccaataca gagacttcgc ttggattctt
      421 ggattagtgg cggagttact acagcggctt taagttattc ttttgacaga accaccagtg
      481 atccactgat ttcaacctgg gcaaccggat caggtcaaat taacttatca gataacatct
      541 ctataattaa agaaggaata gcggcactcc aagccgactg gacgattaat catggagatg
      601 attggggtgg taatacctca attgagtatc aaaaaactgc tccatttggt gagtatcttg
      661 atgtttcagg cgctgattat ctgacgttat gggtatataa catgacaagt gcctcgcaga
      721 gtggaaaaac ctctttttgg ttcaatcttc tggaagccgg tggcgatgcc gtctatgatg
      781 ggacttacaa tagcgcggag cattggctat accggatatc aggtgtattt gatcaaggac
      841 ccggctggaa tcagatccgg atgccgctaa ttgaccgtgg tgctgaaaat cctaatgatc
      901 agggttttag cgcgccgggc tggaatggag tagctaacaa tggtattttg gataaagatc
      961 gaatcgttgg ctggcatatt attattcaga ctgaaaatac cgctataggt gattttgtta
     1021 gtgggacgac tctttttgac cagttgcagt tagaaactgg ggagattgtc ggtgagtttt
     1081 cggtaacctt ccaggctgac ttaaccgact ttattgctaa tggctggttt aatcccgcaa
     1141 atccagctga ttctattgtg gttaatggcg atttcaatgg ctgggattct catcagaaaa
     1201 tgttgcccag cgaaagcaat ccgaatattt acctctttac aaccgttgta actggtaatc
     1261 ccggcgatat cgtcggttgg aaattcagaa ttttccctga ttctaaatgg actaataatg
     1321 gttgggaaag tattgaaaac cgagaattaa tattaagaga aaataatatt gtcttggaat
     1381 caattaaacc gatcatttac tatcgtggta atcccattac gcaggatgta accgttacat
     1441 ttacagttga tatgtctaac tttgacggta gttttagtgc agtctctctg caaggcaatg
     1501 ctttaccatt gaattggtcg cccggctcgt taccaatgga tgattccgat aaagatggta
     1561 tttatacagt ggctgtattt tttccaagtg ggactggcaa aactatagag tataaatata
     1621 cagcgcttac cgatacagct tgggtctggg aaagttttgt cggcaatcgc agttttgaga
     1681 ttgatgatag ttcacccgac caaatactag ccgttgatat ttttaatgga tatggtactg
     1741 ctctcaacgc atcggtttat ttaaatggtc agaaagccag actacgtgtg accgacagtg
     1801 ctccaatcaa tccggcagcc aatcccagcg cctatgtaat taccggaaaa gccataacgg
     1861 tcgaaacctg ggtcttcccg ctgaatttac ctgaaaatgg taattgtgct gtgctggttt
     1921 cgcgtcctta ttggaataca gacccctggt acgcttatga actccggatc aacaatttcg
     1981 gtccgaccga tgagccgcgc ttcgatttcg ttgttagcga tggcaatcct ggcggtactc
     2041 ataatggggt catcagttcg aatccgatta cgatcggcga gtggacccat gtagttggaa
     2101 cttatgacgg cagtaatcta cacctctatg ttaacggtga acttgtcggc gaagcatctt
     2161 ttactgccaa tatcggacaa ggagatgcag gattttatct cggtggcacc tggagtaatt
     2221 attttgacgg tgttatagat gaagtaagac tctggaatgt ctgtcgtagt caagcagata
     2281 ttcagaatac aatgaaccag cagctcgtgg gtgatgaagc cgggctagtt ggctattggc
     2341 ctcttgatga agagattgaa gtaaatggtt catatccagt ctgtgccgat attactacta
     2401 atcataacga tcttctaatg cagaatggag ctcggattat atcggcttcg gctccattag
     2461 tagaaccaga aattgctcct gagttccaaa ttcaagaact ctacgggata gcgaatataa
     2521 atttctcata tcaaatactc tgttccggat ggcataagcc aacctttacg cttgtcaacg
     2581 gtcctcaggg cttaattcta aacaatggat gggtgtattg gacacctact actgccggac
     2641 actactcgtt agttctggaa gccagaaatt cagccggtgt aactcaaggg atctaccata
     2701 tctgggtaga ttcgatacta gtaagtacag atactcacaa caataataat gtattactaa
     2761 acgtttacaa taacggattt atcggcaata ctctacccga ccttgacggc aacggctttc
     2821 aatataatgg cacaaacggg ctattcatag gtagtcttat tattgctcag agtgcgaatc
     2881 aggttgcggg gaaaatttac aataacgaat ttggaactct ggaaccggtc cattctgtaa
     2941 caagtatgtt gacaggcttc gatcaggctt ttgaaagtcg ttttaatgat acacggaaat
     3001 taaatcctat cggtgtcgaa gttgtccaaa gaactcattc caaatctacc aatcctgatc
     3061 aggattacgt tttagtggaa tatgaaattt acaacaagtc ggggaggcaa ttaacggatt
     3121 tgtatattgg tctagcaatg gattgggatg ttggaaacga cgccacccac aattttagcg
     3181 ggtatgatgc cgagaaaaaa cttagctatg cttttttaaa taatgaaacc ccttattttg
     3241 gaataactgc tctaaccggg gatgttaccg gttataaaac atggctggct aattcgggag
     3301 aggatgaaaa ttccgatgaa ttcctatatc tggcaatgac ccaattttct gacctacctt
     3361 cctcggcagg tgatattcga aatgttttgg gaactggac
//
LOCUS       DAOOMU010000144         3269 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_35618_length_3269_cov_23.744866, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000144 DAOOMU010000000
VERSION     DAOOMU010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35618_length_3269_cov_23.744866"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1028
                     /locus_tag="PLI55_09570"
     CDS             54..1028
                     /locus_tag="PLI55_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPB01118.1"
                     /translation="MSKTFISDLKENDRVTSFFGVFSKAFRRTKENKIYLDLILVDRT
                     GKINAKIWDDVENLAEQFEAGDAVKVQGTVTIFNNELQIKVEKIRRVIPERDRAEGYD
                     AGDLILSTSKNIEGMWEDLQQIIQSLKNPFLKAVVGAIYEKYGEQLKIHPGSMILHHA
                     FRGGLLEHIYTMCQVAEGVCQAYTELDRDLVMSGILLHDIGKLVELEAGMAVSYTDAG
                     YFLGHIVLGRDIVRDQCLAINGFPEILRLQLEHILLSHQGKHEFQSPREPQFLEALVV
                     YYIDELDTRFNQMKNDIATDLNEGKWTAKGGYFKRVLFKGERESDQNS"
     gene            1086..1646
                     /locus_tag="PLI55_09575"
     CDS             1086..1646
                     /locus_tag="PLI55_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01119.1"
                     /translation="MNSRIRKITYSAIFIAIIVGVGYALAFVPNVELVTALIFLSGVL
                     MGWKSGLIIGGLGEFLFSALNPLGSGLIFPPMLIAQVLAMVIIGGVGGLCRNFVLSWQ
                     PRLRNFIAIGLLGGLLTIFYDICVSIAYPLSAGFNWRGLVTTLAAGLAFSIIHIIVNL
                     IVFLFLVPVTAQRIARAIPYFREERR"
     gene            1648..>3269
                     /locus_tag="PLI55_09580"
     CDS             1648..>3269
                     /locus_tag="PLI55_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01120.1"
                     /translation="MKALRLLLIVGWISIFLEAGMSDNPYQADSSRVETETQSVGIYN
                     IADHIFERSNYIFLPYDDWGVKGVIQTFGLPVTALTMRNNGLLMTDPVYGQAPLTWLN
                     PRFDQIELEATAIKMTPRYFLRDKVFSRFDYYRGDYGFMNFSMLISGKLADTTTVWRF
                     VGENLGYDSYYGNLGAKLSQTGESLSQIYRLDLRAFWKSWQIDLNAAYQKYLPGYVKV
                     SPTTSSAYLSPSWNGNVKEYRAALGGIAIRASERDSTALGFQQNNFIYGQYVKDPRFG
                     FKGVADQSEFIFYHDRQISHFRLAMHFNPVTRSVTLRNGFYERQNLAAGGIGISSKKI
                     KSHWSLYLGAIDQYFDGEFHLRYHLSPKNQVGGRIASTYALYPLIYHLDFPVNQPISK
                     EGFQYQIGTIDFLHASRSLQLSLAFNQVNSNFYVPYKSAINDTTLIYLPQKLDAAYIT
                     ADGRLILPWRMTLTVRGLISNNEEGVWLQGWGQIRQEIDLFQNNLRLYVAGELTYWDM
                     SKNLGWFEELRGIGQFGTEYFTNNQLNIVARIG"
BASE COUNT          878 a          630 c          779 g          982 t
ORIGIN      
        1 cttgactttg ggagtctaat tagctttaat tctggaaaat tcagagggaa aaaatgagca
       61 agacatttat tagcgatctc aaagaaaatg accgggtcac ttcgtttttt ggcgtattct
      121 cgaaggcatt tcggcggacg aaggaaaata aaatttatct cgacctgatc ttagttgacc
      181 gcaccggcaa aataaatgct aaaatctggg acgatgtgga gaatttagcc gagcaattcg
      241 aagctggaga cgccgtcaaa gttcaaggca cagtcacaat ttttaacaat gaacttcaga
      301 tcaaggtgga aaaaattcga cgcgttatcc cggagcgtga ccgcgccgaa ggctacgatg
      361 ccggtgattt aatacttagt acgagtaaaa atatcgaagg tatgtgggaa gatttacagc
      421 aaattatcca atcgttaaaa aatcccttcc tcaaggcggt cgttggcgct atttatgaaa
      481 aatatggcga acagttgaaa atccacccag gctcgatgat tctccatcat gctttccggg
      541 gcggattatt ggaacatatc tatacaatgt gtcaggtagc agaaggagtt tgtcaagcct
      601 atacagaact cgatcgcgat ttggtaatga gcgggatttt gttgcacgac atcggcaagt
      661 tagttgagtt ggaggctgga atggcggtat cttataccga tgccgggtat tttctcggtc
      721 atattgtatt gggacgagat atcgtccgtg atcaatgcct agcaataaat ggttttcctg
      781 aaattttacg tttgcaactc gagcatattc tcctttctca tcagggcaag cacgaatttc
      841 aatcgcctcg tgaacctcaa tttttagaag cattggtagt ttattatatc gacgaactcg
      901 atacgcgctt taatcaaatg aaaaatgaca ttgcaactga ccttaatgag ggtaaatgga
      961 ctgccaaagg tggttatttc aaacgggtat tattcaaagg cgaacgcgaa tccgaccaga
     1021 atagctaaag aatccttgaa ttatcggcgt cgctttgtaa attgcagcga cttaatggtg
     1081 gttttatgaa ttcaaggatt agaaaaataa cttactcggc aatttttata gcaattatcg
     1141 tcggggtcgg ctacgcgctg gcattcgttc cgaatgtcga attagttacg gctttgatat
     1201 ttttatccgg ggttttgatg ggctggaagt ccgggctgat tatcggcgga ttgggcgaat
     1261 ttttgttctc ggctcttaat ccattgggct cgggactaat ttttccgcca atgttgattg
     1321 ctcaagtgct ggcgatggtc attattggtg gtgtcggcgg actttgccga aattttgtac
     1381 taagctggca accgcgactg agaaatttta ttgcaattgg attactcggt gggcttttga
     1441 caatttttta tgatatttgc gtttctattg cctatccctt atcagcggga tttaattggc
     1501 gtgggttggt gacgacctta gcggctggtt tggcattcag tattattcac ataatcgtta
     1561 atctgattgt ttttctgttt ttagttccgg taacggcaca gcggatcgct cgagcaatac
     1621 cttatttcag ggaagaaaga cgctgaaatg aaagctctca ggcttttgct gattgtcggt
     1681 tggatttcaa tttttttaga agctggtatg agtgacaatc cgtaccaagc ggattccagc
     1741 cgggttgaaa ctgaaaccca atcggttggg atttacaata ttgccgacca tatttttgag
     1801 cgaagtaatt atatttttct gccttatgac gattggggag tcaaaggcgt aatccagact
     1861 ttcggtttgc cggttacggc tctcactatg cggaataatg gtctgctgat gactgatccg
     1921 gtttacgggc aagcgccatt aacctggctg aatccgcgtt tcgatcagat tgaattggaa
     1981 gcgactgcca taaaaatgac accgcgctat tttttgcggg ataaagtatt ttcgcggttc
     2041 gattattacc ggggcgacta tggctttatg aattttagta tgttgattag tggtaaatta
     2101 gctgatacta cgacggtctg gcgttttgta ggcgaaaatc tgggctatga tagttattat
     2161 ggtaatctcg gagcaaaatt atcccagacc ggcgagagtc tttcgcaaat ttaccgactg
     2221 gatctgcgag ctttctggaa aagctggcaa attgacctga atgccgctta tcaaaaatat
     2281 ctgccgggtt atgttaaagt ttctcctact acatcgtcgg cttatttgtc gccttcttgg
     2341 aacggtaatg taaaagaata tcgcgccgca ctgggtggta tagctatcag agcaagtgaa
     2401 cgcgattcaa ccgcgcttgg ttttcagcaa aataatttta tttacggaca atatgtcaaa
     2461 gatcctcgat ttggattcaa aggcgtggct gatcaatcgg aatttatatt ttatcacgac
     2521 cgacagattt cccacttccg cttagcgatg cactttaatc ccgtaactcg ctctgtaact
     2581 ctccgtaatg gcttctacga gcgtcaaaat ttagcggctg gtggcattgg catttcatcg
     2641 aaaaaaatta aaagtcattg gagtctttac ttaggtgcga tcgatcaata tttcgacggc
     2701 gaatttcatc tccggtatca cctgagtccg aaaaaccaag tcgggggacg gattgcctcg
     2761 acttatgccc tttatccgct aatttaccat ttggattttc cggtgaacca gccgattagc
     2821 aaggaaggct tccaatacca gatcggaacc attgactttc tacacgccag tcggagttta
     2881 caattaagtt tggcatttaa tcaagtgaac tcgaattttt atgttcccta taaatcggca
     2941 atcaatgaca cgactttgat ttatctgccc cagaaattgg acgcggctta cataaccgcc
     3001 gatggaagat taatcttgcc atggcgtatg acgctaacgg tgcgcggttt aatttccaat
     3061 aacgaggaag gcgtctggtt gcaaggctgg gggcaaatcc gacaggaaat tgaccttttc
     3121 cagaataatt tgcgattgta tgtcgctgga gaattgactt attgggatat gtcgaaaaat
     3181 ttggggtggt ttgaagaact gcgaggaata ggccaattcg gaacggaata ttttactaat
     3241 aaccaattaa atatagttgc ccgaattgg
//
LOCUS       DAOOMU010000145         3216 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_36417_length_3216_cov_11.323632, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000145 DAOOMU010000000
VERSION     DAOOMU010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36417_length_3216_cov_11.323632"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..759)
                     /locus_tag="PLI55_09585"
     CDS             complement(388..759)
                     /locus_tag="PLI55_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01121.1"
                     /translation="MAKLILPRPALLIAQLQMMSAALAKYAETYVPVSPSKDELDACV
                     TELTSALQNQVQAAGIAEKATKDLYAVRDRSVDLLRRMRDALYAHFGKYDPRLVEFGL
                     DTMKARKPKNGNGSDETGNLQ"
     gene            1018..2046
                     /locus_tag="PLI55_09590"
     CDS             1018..2046
                     /locus_tag="PLI55_09590"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPB01122.1"
                     /translation="MQRLISILRREREKAHLTQNDLAQRMNCSRDTIARIETGRQVIN
                     LDQLLGYIRICQINPNVILKAFEPEKPAEFAFMLRMHNVHYITDQTRQQFADWYSACE
                     KSRTNEHIIIRELPDLKYDESQDPRHSGEHHAIVTRANWNLGSVPIQDPVSLISDLGI
                     FIQGADLGNNDLFALTGKRNDSNQYGMIINTNSAITIERQRFSIFHELAHLIAHRDDF
                     SIDYNETGKGRSKDPREIYADAFAGAFLVPQDELERFIQIANARSPLNLGSWILEAKR
                     YFGVSYQTILLRLKSIGYFKNDRQFGIIFGKLRKIYGSNEPNPITTPLSFINTPDWPD
                     TIVFKFSS"
     gene            complement(2150..2923)
                     /locus_tag="PLI55_09595"
     CDS             complement(2150..2923)
                     /locus_tag="PLI55_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01123.1"
                     /translation="MKESKFFSILVLAIAFMLTLTINSFAQEEMPPVEEEVVEEAVEE
                     EVVTEEPDVFMPSWIAKAYLNFGPIEPWGDPGTGALFNMGMRIQGGAQLNIGNWVQLP
                     MVLQPLTAEVMVGYGMWNIKDDYTGVYVDGKTNVISVLALGRYDLTDLIMKAIGFEYP
                     ALGIFVVAGLQYNYQSWDFPNWSREFDPASSFGLNLGVGVKYNLQSLVGKPVEIDLRF
                     TQGVFVMGDVKDQNGDPFYPDADYQHTENGLLLGIAYPF"
BASE COUNT         1010 a          632 c          597 g          977 t
ORIGIN      
        1 aaacgattta gccaaacatt ttacccgttt aacaaagtgg caaaacgatt tagccaaaca
       61 ttttaccggt caaacaaaat ggccaaatgg tttatcaaaa ttgataagtg agtaaaatgt
      121 ttttccaatt ggttaaaatt gacaacgcta gggcaattta tcataatgta aattataggg
      181 tacttaactt aattacgcat tactatggtt atgatatttt gatctagagc cgagaatagt
      241 attattttct cctattgact aaagattaaa taaccaatat atttcagata gaattgattt
      301 ttgatatttt agttatttag tataaaaagc aaaacctccc agaactggag ttccgggagg
      361 tttcagagac gaggaatggt tcagttgtta ctggagatta ccagtttcat ctgaaccatt
      421 tccatttttc ggcttacgtg ccttcatagt atcgagcccg aactcaacca atcgtgggtc
      481 atatttaccg aaatgggcat aaagcgcatc tcgcatacga cgcaaaagat caactgaccg
      541 atcgcgtacg gcatataagt ctttagttgc tttttcagca ataccagccg cttgtacctg
      601 attttgaagt gcactggtta attccgtaac gcaagcgtcg agttcgtctt tcgacggact
      661 tactggaacg taagtctcgg catattttgc caatgccgcc gacatcatct gtaactgcgc
      721 aatgagcagc gcaggccttg gtagaatcaa tttagccatg gttctcacct ccttcctaat
      781 ttgttcctgt aaaaaaagcc agaaaaacca gaaactttgg gttaaagtac aattgggctt
      841 gtttttattt tttcgataat gtcaaaaagc aaatatcgtt cgactaccct taatatatct
      901 attaattgcg ttatttgcaa gtaaaaataa aatatatgtg tgtttatcgc acaatggtct
      961 tgcatatcat caagtattgc tttaaattaa gtcagcaatg aaaaaggagt aaaaaaaatg
     1021 caacgtctaa ttagcatcct gcgtcgggag cgggaaaaag cgcacctgac gcaaaatgat
     1081 ttggcgcaac gtatgaattg ttctcgggat acaatcgctc gcatcgagac cggacgacag
     1141 gtcattaatc tcgaccagtt gttgggatat attcgtattt gccagatcaa tccgaatgtt
     1201 attttaaagg cttttgagcc tgaaaaaccg gctgaatttg cctttatgtt gcgtatgcat
     1261 aatgtccatt atattactga ccaaaccaga caacagtttg ccgattggta ctcggcttgt
     1321 gaaaaaagtc ggactaatga acatatcatt atacgagaat tacctgatct taaatacgat
     1381 gaaagccagg accctcgtca cagtggcgaa caccatgcaa ttgtcacccg cgcaaattgg
     1441 aatttaggta gcgttcctat tcaagacccc gttagtctga ttagtgatct tggaattttt
     1501 atccagggtg ccgatttagg caataacgat cttttcgctt tgaccggtaa gaggaatgat
     1561 tctaaccagt atgggatgat tatcaatact aatagtgcca ttaccatcga acgccagaga
     1621 ttttccattt ttcatgagtt agcccatctg atagcccacc gcgatgattt ttccattgat
     1681 tataatgaaa ctggtaaagg aaggtctaaa gatcccagag aaatttacgc tgatgccttt
     1741 gccggtgcgt ttttagttcc tcaagatgaa ttggagcgtt ttattcaaat tgctaatgcc
     1801 agaagccccc ttaatcttgg tagctggatt ctggaagcca aacgttattt tggagtcagt
     1861 tatcaaacca tattactgcg actaaaatct attggatatt ttaaaaacga ccgtcaattc
     1921 gggataatat ttggcaaatt gcgaaaaatt tacggttcta atgagcctaa tccaattacc
     1981 acaccgctga gtttcatcaa tacgcccgat tggccagata ctatcgtctt taaattctcc
     2041 agctaaaata gcaatgtgct attgagatta agttttaatt ggctttaaaa aagaaaaagt
     2101 cccgaaaatc gggacttttt ttattaacac tgatattttc taaaagacct tagaatgggt
     2161 aagcaatacc aagcagtaat ccgttttcgg tatgctggta atcagcatca ggatagaacg
     2221 gatcgccgtt ctgatcttta acatcgccca ttacaaaaac gccctgagtg aagcgcaaat
     2281 caatttcgac tggcttgccg accaagctct gcaggttgta ttttacccct acgcccagat
     2341 ttaacccgaa ggaagatgcc gggtcaaatt ctctactcca attcgggaaa tcccaggatt
     2401 gataattgta ctgcagacca gcaacaacaa aaatacctaa tgctggatac tcaaatccaa
     2461 tggctttcat gattaaatcg gttagatcat aacgtccgag tgccagaact gagatgacat
     2521 tggtttttcc gtcaacatat acgccagtgt aatcgtcttt aatattccac atgccatagc
     2581 cgaccataac ttcggcagtc agcggttgga gaaccattgg taattgtacc cagttgccaa
     2641 tattcagttg agcgccaccc tgaattctca tacccatatt gaataatgca ccggttcctg
     2701 gatcgcccca gggttcgatt ggaccaaaat tcaaataagc cttggcaatc cagctaggca
     2761 tgaaaacgtc tggttcttca gtaactactt cttcttcgac tgcttcttca acaacctctt
     2821 cctcaacggg aggcatctcc tcctgtgcaa atgaattgat tgttaaagtg agcataaaag
     2881 caatagcaag aactaagata gaaaagaact tactttcctt cacaataacc tcctttatta
     2941 aatattgatt ttctcatagc cattttaact tatagttaat atatatatag ttttttaata
     3001 tgtcaataga attttttaat tagtatttaa aaataaaata attttatccg gcttatattt
     3061 accataaaag gaatacaagt aattgatatt atatgaataa gaataaaatg gtgatttata
     3121 tcacggttct tctggaggaa ttaatgagga atttttagac aggattaata agatatttct
     3181 ttattcggta aatgagtaaa atcccaaact ccaaat
//
LOCUS       DAOOMU010000146         3079 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_38571_length_3079_cov_12.323743, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000146 DAOOMU010000000
VERSION     DAOOMU010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38571_length_3079_cov_12.323743"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2641
                     /locus_tag="PLI55_09600"
     CDS             <1..2641
                     /locus_tag="PLI55_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HPB01124.1"
                     /translation="MKSCFFKFIESWYFAIPMIAFLGLFASLNAADKKGLVLNDQDYF
                     EMPGLNVLFFHNTFSEGHQGGLEIIQHENRVATNGEIRLNATPGQWQPTAKLGEGLQK
                     RGVSPQTLAIESRKVDRKKNEISIPCSFPDESLNRKGFNPIIYPDLHIQYTIRVRAKG
                     QSIFVTVDFDEPIPPEWVGKIGFNLELFPGNLYGKHYYMDGKSGIFQRQPEGEAVLND
                     AGEAEAPALAEGQVLVVAPESDLFRMKIESKGSQLVLLDGSLQHNNGWFVVRSLVPSG
                     VTKNAIEWVITPNVIPDYKYEPVIHISQVGYHPNQKKIAYIELDKRETNIQNVTLYKI
                     TDNGEYKVITDRKPKEWGQYLRYKYALLDFSDIKESGIYVVKYGNKVSNSFQIDPDIY
                     KHNVWQPTLDYFLPVQMCHMRVNQKYRVWHGACHMDDALMMPLNIRHFDGYNNEGMTS
                     TLTQYKPLDHVPGLNVGGWHDAGDYDLRVESQAQAVLTLALAYEEFGIEWDNTFVDQE
                     NHHVEIHQPDGKPDILQQVEHGVLAILAGYRQFGRLYRGIICPTLRQYVLLGDGSTMT
                     DGKVFDDPEAKAKAANIDELWYKKVANRYSKIFDPELNAHEIQVYIPDLDDRLVFTDE
                     KADLQLVGATGLAAAARVLKGYNDELAEECLTVAEALYEANKNQGSVSEFMRDNARVN
                     ALCELIITTNSQKYKDELCGMKDEIVKNFQLSATAMARVMPYIDCEEFKTAIYTAAKE
                     LKPELDEMMSETPFGCPTRTPEYLGYSAYFLHKAWPDVFPPDHLYSVVNFLLGCRPGS
                     TTNSLVSGVGANSPTIAYGANRADWSYIPGGTFWAAVNLVRPDFPEDKDWPFLWQERE
                     YIIGGACTYMFIVMAANHLLTGN"
     gene            2700..>3079
                     /locus_tag="PLI55_09605"
     CDS             2700..>3079
                     /locus_tag="PLI55_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HPB01125.1"
                     /translation="MSKKSKILAIDKTGIIGLLLVFTALNLNAQLVTNGSFEATALGV
                     VSGRDVAGWVIEKSAGSADFEIVDSVVQHGNRALKVVVTEAGSNQWDLQIVADSIPVV
                     PGGNYRFTVWAKAQQAGAQVNFTVG"
BASE COUNT          871 a          616 c          752 g          840 t
ORIGIN      
        1 tatgaaaagt tgttttttta aattcattga atcctggtat tttgcaattc ctatgatcgc
       61 ttttttagga ttgtttgcct ctttgaatgc cgccgataaa aaaggtctgg ttctcaatga
      121 tcaggactat tttgaaatgc ccggactgaa tgttcttttt ttccataata ctttctccga
      181 aggacatcag ggcggtctgg aaattattca gcatgaaaat cgcgtggcga ctaacggaga
      241 aattcgacta aacgccacac ccgggcaatg gcaaccgacc gctaaattgg gtgaaggctt
      301 acaaaaacga ggcgtcagtc cccagactct ggcgattgaa agtcgtaagg ttgaccggaa
      361 aaagaatgaa atcagcatcc cttgcagttt tccggatgaa tctttgaatc ggaaaggatt
      421 caatcctatc atttatcctg atcttcacat ccaatatact attagagtaa gggcgaaagg
      481 tcagtccatt ttcgtaacgg ttgacttcga cgaaccgatc ccgccagaat gggtggggaa
      541 aattggattc aatctggaat tattcccggg caatttgtac ggtaaacact attatatgga
      601 cgggaaatcc ggtatttttc aacgccagcc ggaaggcgaa gcggtgctta acgatgccgg
      661 tgaagccgaa gcgccagctt tagccgaagg tcaggtgctg gtcgttgcgc cggaaagcga
      721 tctctttcgt atgaaaatcg aaagtaaggg gagtcagctc gtattgcttg atggtagttt
      781 gcaacataat aacggctggt ttgtggtgcg ttcgctggtt ccaagcggcg ttactaaaaa
      841 tgcaattgaa tgggtaataa cccctaatgt cattcccgac tataaatatg aaccggttat
      901 tcatatttcg caggttggtt atcatcctaa tcagaagaag attgcctaca tcgaattgga
      961 taaacgcgaa acgaatattc aaaatgtgac attgtataaa attaccgaca atggtgaata
     1021 taaggtgata accgaccgga aaccaaagga atggggacaa tatctccgat ataaatatgc
     1081 attactggat ttttccgata tcaaggaatc tggtatctat gtggtgaaat atggcaataa
     1141 ggtcagtaac tcatttcaaa ttgatcctga tatttacaag cacaatgtct ggcaaccgac
     1201 gctggattat tttttaccag ttcaaatgtg ccatatgcgc gtgaaccaaa aatatcgcgt
     1261 ctggcatggc gcttgtcaca tggatgatgc cctgatgatg ccgctgaata ttcgtcattt
     1321 cgacggttat aataatgaag gaatgacctc gactttaact caatataagc ctttagatca
     1381 tgtgccgggt ttgaacgtcg gcggttggca tgatgccggt gattatgatc ttagagtgga
     1441 atcccaagct caagcggttc tgaccctggc gctggcttat gaagaattcg gaatcgagtg
     1501 ggataatact tttgttgatc aggaaaatca tcacgtcgag attcaccagc cggatggtaa
     1561 gcctgatatc ctacaacagg tagagcatgg tgtgcttgcg attctggcgg gctatcgcca
     1621 gttcggtcgt ctttatcgcg ggattatctg tcctacttta cgccagtatg tattgctcgg
     1681 tgacgggtcc acgatgacgg acggcaaggt cttcgacgat ccagaagcca aagccaaagc
     1741 tgccaatatt gacgaattgt ggtacaaaaa agtagctaat cgctactcta aaattttcga
     1801 tccggaattg aatgcccacg aaattcaagt ttatattccc gatttagatg accgtctggt
     1861 ttttaccgat gaaaaggcgg atttacaact cgtgggagca accggtttag cggctgccgc
     1921 tcgcgttttg aaagggtata acgacgaact ggcagaagag tgcctaaccg tcgccgaagc
     1981 gctgtatgaa gccaataaga atcaaggcag cgttagtgaa tttatgagag ataacgctcg
     2041 tgtaaatgct ttatgtgaat taatcataac gaccaattcc cagaaataca aggacgaatt
     2101 gtgcggtatg aaggatgaaa ttgtgaaaaa ctttcaatta agcgctacgg caatggcgcg
     2161 agttatgcct tatattgatt gtgaagaatt taaaacggca atctatacag cggcgaaaga
     2221 attaaagccg gaattagatg aaatgatgag tgaaacgcct tttggttgcc cgacccgcac
     2281 cccggaatat ttgggatatt ccgcctattt tctgcataaa gcctggccag atgtgttccc
     2341 gccggatcat ttatactcgg tagtcaattt cttactcgga tgccgaccgg gtagcactac
     2401 caatagcctg gtttccggag tcggcgcaaa ttcaccgacg attgcctatg gagcgaatcg
     2461 cgccgattgg agctatattc ccggtggtac attttgggcg gctgttaatc tggttcgacc
     2521 ggacttcccc gaggacaaag attggccgtt tctatggcag gaacgcgaat atatcatcgg
     2581 cggcgcctgc acctatatgt tcattgtaat ggcggcaaat catctcttga caggtaattg
     2641 attaaatgtt atatttaaaa atgtaggata agaataacga aaaaaataag gagaacaata
     2701 tgtctaaaaa atcaaaaatt ttagcaattg acaaaactgg catcattggt ctgctattgg
     2761 tttttacggc tttaaattta aacgcccagc tggtcactaa tggtagtttt gaagcgaccg
     2821 cgctcggcgt agtctccggt agagatgttg ctggctgggt aatagagaaa tcagccggta
     2881 gtgccgattt cgaaattgtt gacagcgtcg tccagcatgg gaatcgggct ctaaaagtcg
     2941 ttgttactga agctggatca aatcagtggg atttacaaat agtggctgat agtattccgg
     3001 tagttccagg cggaaattat cgctttaccg tctgggctaa ggctcaacaa gccggggcgc
     3061 aggttaattt taccgtcgg
//
LOCUS       DAOOMU010000147         3059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_38893_length_3059_cov_14.188083, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000147 DAOOMU010000000
VERSION     DAOOMU010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38893_length_3059_cov_14.188083"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="PLI55_09610"
     CDS             complement(<1..1451)
                     /locus_tag="PLI55_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB01126.1"
                     /translation="MNLLFFLDTFAASISGTVKVIESLEPMSGVNVFLANTNLGCSSD
                     PNGYFTITGIEPGQYQLVASHIGYGVYQEFIEINAGDRRFIDIRLQALPVLIDTVSVF
                     GERRFRRELEGDLLNISSVELNLAPKLGEPDLFRVIETFPGVTTVNDYNMGLHIRGGN
                     SDQNLILLDGMPIYNPFHLVGIFSTFDESAIQSANVSKSGIDPVYGNRLSSVIDVNVR
                     DGTAAEHAGYINLSFLSSKVRAEGPLPFGSYLLTVRRTYADLFVNTVTKIISSDIILP
                     YHFIDGMGKVVIKPGERNRLEITSYLGKDVYDMRVIDGDDSSGVYNWQNKALGFNYSN
                     VINPQMVFYLRGSVSRFRAEWVPEDTTKSEMIDNVFTGKTINAYFRYENAIIGQIKVG
                     AEYCNNDYHLKTKGFSYQPIYFDKNQSLENSAFFSINRQLGALFNVGGGLRMTHFSIQ
                     DTLTFSPQLSLGWKIAPSHEIQLHWGKYHQDLMT"
     gene            complement(1557..2219)
                     /locus_tag="PLI55_09615"
     CDS             complement(1557..2219)
                     /locus_tag="PLI55_09615"
                     /inference="COORDINATES: protein motif:HMM:NF022745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3103 family protein"
                     /protein_id="HPB01127.1"
                     /translation="MKRKWRTDGESAILVAYTPLTTDDMEWEEIYAYDADLKEYILDA
                     KTEPDYPVIVVGINERIEYNKATIVQTADKIIAKTAEIRQSEEILDSIRIAIDGEGWP
                     NGDPEIYIVAAGTNTTWDRRERVNLASVNKINKWYPIDGALFSWETKYWGTHTGYGVL
                     EYDPDGYNYSVTILSEDVEVEVKKKSDGNDEFIGNISSIPYDHTNGVIWLLDNAKIGL
                     SY"
     gene            complement(2304..2648)
                     /locus_tag="PLI55_09620"
     CDS             complement(2304..2648)
                     /locus_tag="PLI55_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01128.1"
                     /translation="MDRKSALKVRESGQDWAEKNICTINANPAKGKVQPVSAGNSKFQ
                     ASNSKEAPQTKYQTLPIPDRCGHWYLKFIWYLVLETWYLIPGIRTFVFANNQKKGVYY
                     VMSVRKAEARSQ"
BASE COUNT          892 a          675 c          576 g          916 t
ORIGIN      
        1 gtcattaaat cctgatgata ttttccccaa tgtaattgaa tttcgtgaga tggagcgatt
       61 ttccagccca gagataactg cggtgaaaat gtcagcgtat cttgaataga aaaatgcgtc
      121 attcgcagac cgccgccaac attgaacagc gctcccaact ggcgattaat gctgaaaaag
      181 gctgaatttt ccagggattg gtttttgtcg aaatatatcg gctgatagct gaatcccttg
      241 gtcttcaggt ggtaatcatt attgcaatat tctgccccga ccttgatctg cccaataata
      301 gcattttcat accggaagta agcgttaatc gtcttaccgg taaagacatt atcgatcatt
      361 tcagatttcg ttgtatcttc gggaacccat tcagcccgga accggctgac actgccgcgt
      421 aaataaaaaa ccatttgcgg attgatgaca ttggaataat taaagccgag cgccttattc
      481 tgccagttat aaacccccga actgtcatct ccatcgatca ctcgcatatc ataaacgtct
      541 tttccaagat aggaggtgat ttccaggcgg tttcgctctc cgggtttaat gacgactttt
      601 cccatcccgt cgatgaaatg atacggcaat attatatcag acgatatgat tttggtaaca
      661 gtattgacga acaaatcggc ataagtccgg cgaaccgtca gaagataact accgaatggc
      721 aacggtccct cagcccggac tttggaactc aggaaactga gattgatgta gccggcatgt
      781 tcggcggcgg ttccgtcccg gacattgacg tcaataactg aactgagacg attgccatag
      841 accggatcga ttccgctttt agaaacattg gcagattgaa tcgccgattc gtcaaaagtc
      901 gaaaatattc ctaccaggtg aaaaggatta taaatcggca taccgtccag caggatcaga
      961 ttctgatcgg agttgccgcc ccggatatgc aaacccatgt tatagtcgtt cacggtggtc
     1021 acaccgggaa aagtctcgat gacccgaaat agatcgggct cgccgagttt cggggcgaga
     1081 ttcaactcga cggaactaat attcagcaga tcgccttcga gttcgcgtct gaatcgccgc
     1141 tcgccgaata ccgaaaccgt atcaatcaag accggcaaag cctgcaaacg aatatcaata
     1201 aaacgcctgt ctccggcatt gatttcgata aactcctgat aaactccata accaatgtga
     1261 ctggcaacca gttgatattg gcccggttca attccggtaa tcgtaaaata accattggga
     1321 tcggaactgc atcccaggtt agtgtttgcc aggaaaacat tcacacccga cattggttcc
     1381 agcgattcga taaccttaac agtaccggaa atgctggcgg caaaggtatc taaaaaaaat
     1441 aataagttca atattgaaat agtcaataca gtatcgaaga attttagcaa catagacaac
     1501 tcacaattta attgataaat ggaggcgata tttttatacc gcctccatta ttttcatcaa
     1561 taactaagtc ctatttttgc attgtcaagt aaccagatta ctccattagt atgatcataa
     1621 ggaatactac taatatttcc aataaactca tcatttccat cactcttttt tttgacttct
     1681 acttcaacat cttcgcttag gatagtaacg gaatagttat atccatctgg atcatattca
     1741 agtactccat atccggtatg tgttccccaa tattttgtct cccaactgaa aagagctcca
     1801 tcaatagggt accatttatt aattttattc acacttgcta agttcaccct ttctcttcta
     1861 tcccaagtcg tattggttcc tgctgctact atgtaaattt ctgggtctcc gttaggccat
     1921 ccttcaccgt ctattgcaat ccttattgaa tctaatattt cctctgattg acgaatttct
     1981 gctgttttag caattatctt atcagcggtt tgaactatag tcgctttatt atactctatt
     2041 ctttcattta tccccaccac tattaccgga taatcaggct cggttttggc atccagaata
     2101 tattctttca gatcggcatc ataggcataa atctcttccc attccatatc atcagtagtt
     2161 agaggagtat aggcaaccag aatagccgat tctccatctg ttctccattt tcttttaatt
     2221 tcaaaaaaat cttattaaaa tcgtgcggaa ttagtgtaat ctctgctgag attacgattg
     2281 ttcggcccgg agaaaatgcg caatcattga ctccgggctt ctgctttcct gacggacatg
     2341 acatagtata ctcctttctt ctgattatta gcgaatacaa aggtccgaat gccgggaatt
     2401 aaataccagg tttcaagcac caaataccaa ataaatttca aataccaatg tccgcaacga
     2461 tccgggattg ggagagtttg atatttggtt tgtggtgctt ctttggaatt tgatgcttga
     2521 aatttggaat ttcctgcaga aacaggttgt acttttcctt tcgcaggatt tgcgttgata
     2581 gtacagatat ttttctcggc ccaatcctgg ccggattccc ttactttcag ggctgatttt
     2641 cgatccattc tatccctctt gatagaaatt tacattcatt aaattgccga acaaaccaaa
     2701 aactctgaga aaaaaaccaa ctacactcag ttgaagaacc gctaaacggg gagaataacg
     2761 cttcttcgca agcccaatta gaaataacgg ttaaatttta tatagtattt ttgatttagt
     2821 caagaactat tttaggatct tgaaactatc tgttttattt ttcaattaat ttatattgac
     2881 attgcttaag tcagctaatc atacgatagc ctttccaggc tgtcggcctg gcaatataat
     2941 tcgtaaataa aatagacagg aatagacagg cggacatgcc tgcaccgtat gataatagat
     3001 aggaatagac aggcaagaat gcctgtccta cggctaatat aattttactt ttaacaatt
//
LOCUS       DAOOMU010000148         3039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_39226_length_3039_cov_18.387399, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000148 DAOOMU010000000
VERSION     DAOOMU010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39226_length_3039_cov_18.387399"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="PLI55_09625"
     CDS             complement(<1..294)
                     /locus_tag="PLI55_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005898336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HPB01129.1"
                     /translation="MSLKSKVLLIITISIICSCSPNLYQLKVVNNGVITWRTETGEKI
                     VTRFYSLSDNSLKFVKVVLPGGKEYTLPQVVSASGVRYTDEREIVFWMKGETAF"
     gene            complement(397..834)
                     /locus_tag="PLI55_09630"
     CDS             complement(397..834)
                     /locus_tag="PLI55_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01130.1"
                     /translation="MKKLVLSAVVVMFLMASLSYGFGLGIMLGEPSGLSGKYWMSEKN
                     AIAGGLAWSLSHDYMHVHADYLIHNNNLINVGGTILPFYYGLGAGIGFGDDFHLGARI
                     PVGIEYFFADRKFDVFFELVPVVGLLPDFGFGVSGAIGLRYNF"
     gene            complement(972..>1252)
                     /locus_tag="PLI55_09635"
     CDS             complement(972..>1252)
                     /locus_tag="PLI55_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB01131.1"
                     /translation="TSAFVMGFFLLGVGPLGFQYGAEISYPAPEGTSNGLLLLMGQIS
                     GVLFIFGMDGLKSPQTGSMTLPLVILIALMVLCTLLSVNLKESNLLRK"
     assembly_gap    1253..1352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1353..1753)
                     /locus_tag="PLI55_09640"
     CDS             complement(<1353..1753)
                     /locus_tag="PLI55_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPB01132.1"
                     /translation="MEEVRFKVYPYRWVVLIAFMVIIAVNQLCWITFAPITGRAAQFY
                     GVSDLSIGLLSMIFMIVYIVISIPASWIIDTYGIRIAVGIGAALTGVFGLMRGLLATS
                     YQYVLFAQIGLAIGQPFILNSITKVAARWFP"
     gene            1931..>3039
                     /locus_tag="PLI55_09645"
     CDS             1931..>3039
                     /locus_tag="PLI55_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HPB01133.1"
                     /translation="MKRREFIGTLGITAAAARISQAFPFISQLKTKTTDSGMKVRYEI
                     KALKLKHTWTISRNSSDVKDNVFVYLEKDGITGIGEAAPNIRYDETPESTIELIKKAT
                     PLIERLNPSEFTQFGFALKEIESGQTAGKCAIDMALMDWIGKSLNLPLYKFWGLDPTK
                     APLTSFTIGIDTAEIVKQKTREAEPYKILKIKVGGPNDEEMITTVRSVAPDKPIRVDA
                     NEGWKDKEIAIRKIEWLAKNGIELVEQPLPSEMLEETRWIRERIEIPIIADEAVKTAS
                     DIPKLADAFDGINIKLMKAGGLQEALRMIWLAKSLGMKIMIGCMIESSVAISAATQLC
                     SLIDYADLDGNLLISNDPFSGAIFRDGRQFPADRPG"
BASE COUNT          971 a          696 c          544 g          728 t
ORIGIN      
        1 aaaggccgtc tcgcccttca tccagaacac gatttcccgt tcatcggtat agcgcacacc
       61 ggaagcagat acaacttgtg gcaatgtgta ctctttgcca cccggtagaa ccactttgac
      121 gaatttcaga ctattatccg ataaagaata aaatcgcgtc acgattttct cgccggtctc
      181 agttcgccag gtaattacac cattattcac aaccttcaac tgataaagat ttggcgaaca
      241 actacatatt atagaaatgg taattattaa cagtacttta cttttaagac tcatacagga
      301 aattcaacct atataccgat tctcaacgat aaaataaaaa atagccggag gaatttatcc
      361 tcacggctat ttttcataat tttttaaaat cgcctgttag aaattatatc tcaagccgat
      421 cgcaccacta accccaaaac caaaatcagg caataagccc actaccggaa ccaattcaaa
      481 aaagacatca aatttccgat cagcaaagaa atattcaatc cctactggaa tccgtgcccc
      541 gagatgaaaa tcatcgccaa aaccaatacc tgcgcccaac ccataataaa atggaagaat
      601 agtgccacca acattaatta agttgttgtt atgaataagg taatccgcat gcacatgcat
      661 ataatcgtga ctcaatgacc aggctaaccc accagcaatc gcattctttt cactcatcca
      721 gtatttgcca ctcaaaccgg aaggttcacc cagcataata ccaagaccaa atccgtaact
      781 taaactagcc attaaaaaca tcacaactac tgcacttaaa actaacttct tcataatgac
      841 tcctttctca atttttaaaa tactccaaaa atataaatat caactatgac aatagcaatt
      901 aatatttgcc cttaaaatag ccataaaaat tagaaaattt tgtaattggt ttttgaatcg
      961 gatgcatttt tttatttccg gagcaaattc gactctttca gattaacgct gagcaaagta
     1021 cataaaacca tcagagcgat taaaattacg agtggaaggg tcatcgagcc ggtctgcggt
     1081 gatttcaaac cgtccattcc gaaaatgaaa agcaccccag aaatttgtcc cattagcaac
     1141 aataacccgt tcgaggttcc ttctggagct gggtaggaaa tttcagcccc gtattgaaat
     1201 cccaacggac ctactcccaa caggaaaaat cccataacga aagccgaagt cannnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatcggaaa ccaacgcgcc gcaactttcg
     1381 taatggaatt caaaatgaat ggctgaccaa tggcgagtcc aatctgggca aatagcacat
     1441 attgataact cgtcgccaac aaaccgcgca ttaagccaaa aactccagtt aaagcggccc
     1501 cgattccgac cgcaattcta attccgtacg tgtcaataat ccaagaagcc ggaatcgaaa
     1561 taacaatata cacgatcata aaaatcatag acaataaacc gatactgagg tcagaaaccc
     1621 catagaattg cgcggctcta ccagtgattg gcgcaaatgt gatccagcag agctgattca
     1681 ccgcgatgat aaccataaat gcaattagca ctacccaacg ataaggatat actttaaacc
     1741 gaacttcttc catctggcaa atcctccgat gaattggaaa ttaattgcgc cataaattcg
     1801 actgatttta tgacatttag aattatgaca caagctttag gtagaattat ttacaccaaa
     1861 agaactttgc tcagttatat aaagagatta aattgcaacg taatcggatt taaattttaa
     1921 caggatcgaa atgaaacgac gtgaatttat cggtacttta gggattactg ccgcggccgc
     1981 acgtatatca caggcattcc cctttatatc tcaattaaaa accaaaacca ccgattctgg
     2041 catgaaagtg cgttacgaaa tcaaagcttt gaaattgaaa catacctgga caatttcacg
     2101 caattccagc gatgtgaaag acaatgtttt tgtttatctt gaaaaggacg gcatcaccgg
     2161 tatcggcgaa gccgcaccga atattcgcta tgatgagact ccagaatcaa ctatcgaact
     2221 gatcaaaaaa gccacacctt tgatcgaaag actaaatccc tcggaattta cccaatttgg
     2281 cttcgcgctg aaagaaatcg agtccggaca gacggccggg aaatgcgcca ttgatatggc
     2341 tttaatggac tggatcggca agtctcttaa cctcccactt tataaattct ggggactcga
     2401 tccgactaaa gctcccttga cctcctttac gattggcatt gatacggcag aaattgtcaa
     2461 gcagaaaacg cgcgaagctg aaccatataa aattctcaaa atcaaggtcg gcggtccaaa
     2521 cgacgaagag atgattacta ccgtgcgcag tgtcgccccg gataaaccaa tccgggttga
     2581 cgccaatgaa ggctggaaag ataaggaaat cgccattcgt aaaattgaat ggttagccaa
     2641 aaatggcatc gagctagttg aacaaccttt gccatccgag atgctggaag aaacgcgctg
     2701 gattcgtgag cgcattgaaa ttccgatcat cgccgacgaa gccgttaaga ctgcttctga
     2761 tatccctaaa ctcgccgatg ctttcgatgg cattaacatc aaactgatga aagccggtgg
     2821 tctgcaggaa gccctgcgaa tgatctggct ggctaaatca cttggcatga aaattatgat
     2881 cggttgtatg atcgaaagtt cggtcgccat ttccgccgca actcaactct gttcgctgat
     2941 tgattacgct gacctcgatg gtaatctgct aatctcgaac gatcccttta gcggtgcgat
     3001 tttcagggac ggccgccagt tcccggcgga tagaccggg
//
LOCUS       DAOOMU010000149         3006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_39862_length_3006_cov_3.354117, whole genome shotgun sequence.
ACCESSION   DAOOMU010000149 DAOOMU010000000
VERSION     DAOOMU010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39862_length_3006_cov_3.354117"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3006)
                     /locus_tag="PLI55_09650"
     CDS             complement(<1..>3006)
                     /locus_tag="PLI55_09650"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPB01134.1"
                     /translation="ADPTTAPNVNGIYAIGNTVATGEEVVYIGTATSFTDENLSSETT
                     YYYRVYTVDKAFNYSAGVPVNGTTLGVTGPDDPVPTLSGKTTTSLSFTWNKNGAGDNV
                     MLVYKTTDDIGTPVNGTAYAVGEHLADGAAVLYNGGDQTSYTHTGLTPNTTYYYKFFS
                     VDGSLNYSTGVSSSAKTLMAEPSNHVASFTATEVTYTSIKLTWLDNDGDYPAEQFLVV
                     LETASSFAPVDGQVYSDIGENKWVKYVNHGVQTVTFTGLDDGVTYYAKIWPVSNTSVG
                     GEYYDYKTDGIVPELSQATQVYPSLPFVENFDYEIGELLTNHGWVAHSGSGSQATDVT
                     SGLVFDGYLSSGIGGAANLDNTGEDVHRVFDPVTSGTVYASFIIQTASPNAAGYFFHL
                     GQSTIGTTWLGRVWVNATGDGVAISSGTAPSNYVAITPGKPTLLVVKYDLATKVSSLY
                     VFDTFPTAEPAEPNLIFNETSIFDNVGSVALRQYSASQRVIVDGIRVDTTWAGAVAPA
                     PLDLVSTPTFDPPAGNYYTAQNVTISTTTDGATIYYTTDGSDPTESSTVYTNQIPVSS
                     TTTIKAKAYKTGYAPSSVATAVYSFPIEVANIAALRASPVGSTWYKLIGESILTFKSI
                     TRNAKYIQDTTGAILIDDYSGIITTSYNLYDGITGIIGSLQDYNGMLQFVPVTDPGPA
                     TSTGNIVVPVEVTLAELNTDYQGKLVKVKNVVISATGNFEVSKNYTISDITGSGVIRT
                     QYADLDYLGKPIPTTLQNLTGVVLQYQTTTQLIPRSLTDFEDASLTQYRSKISGNWTD
                     AGTWQKLVGSEWIDATNAPEGFSDYITIKASDTVTVNDTLRIGSGATVKVDRYLKNIA
                     SIISNGTFVFSNGSIYEHAIDKDAIPIATWEEGATCLITGIAGTSAPTGLNQEFYHFT
                     WNCPSQSGNLNFNANLTTIKGDFNVLNTNGKQLRLGSKDLTVNISGDVNIGPNALLTS
                     SGSAGTYTITVNIGGDINISEGMLYLENAG"
BASE COUNT          848 a          674 c          631 g          853 t
ORIGIN      
        1 ccggcatttt ctaagtataa cattccttcc gaaatattaa tatcaccacc aatgtttacc
       61 gtaatcgtgt atgtaccggc tgaaccagag ctggttagca atgcatttgg tccgatattc
      121 acatcaccgc tgatattaac agtcagatct ttactaccca atcgtaactg tttaccgttg
      181 gtatttagaa cattgaaatc gcctttgatg gtggtcagat tagcattaaa attgagattt
      241 ccgctctgag atgggcaatt ccaggtgaaa tgatagaact cctggttaag tcctgtaggc
      301 gctgaggtac cggctatacc ggttatcaaa caggtcgccc cttcttccca ggttgcaata
      361 ggaatggcat ctttatcaat ggcatgttca tagatacttc cattggaaaa aacaaatgtg
      421 ccgttactga tgatacttgc gatattcttt aaataacgat caacttttac agttgcacca
      481 gaacctattc gaagagtatc atttacagta acagtatcac tagctttaat agtaatataa
      541 tcggaaaagc cttccggcgc gttggtggcg tctatccatt cagatccaac caatttctgc
      601 caggtgccgg cgtcggtcca gttgccacta attttgctac ggtactgcgt caatgatgcg
      661 tcttcgaaat cggtgagact acgtgggatt agctgagtcg ttgtctgata ttgcagaact
      721 actcccgtca aattctggag agtagtagga attggtttac ctaaataatc caaatcggca
      781 tattgggtac gtataacacc cgatccagtt atatcggaaa tcgtataatt tttacttact
      841 tcaaaattac cagttgctga tattactaca ttttttacct taaccaattt accttgatag
      901 tcggtattta gttctgcgag agtaacctcg actggtacaa caatgttccc tgtactggtg
      961 gctgggccgg gatcggtcac tggcacgaat tgtaacattc cattataatc ctgcaaagaa
     1021 ccgataattc cagtgattcc atcatataag ttatatgaag tagtgattat gccagaataa
     1081 tcgtctatta aaatagcacc agtagtatcc tgaatatatt tagcattcct ggtaatagat
     1141 ttaaaagtca aaatggattc tccaatgagt ttataccagg ttgacccaac tggtgaagct
     1201 cttaaagccg caatatttgc cacttcgatt ggaaaggaat aaacggctgt ggcaaccgaa
     1261 cttggtgcat acccggtttt atatgcctta gcttttatgg tcgttgtact actaacagga
     1321 atctggttcg tataaactgt actactttca gttggatcac tgccatcagt agtatagtaa
     1381 atagtcgctc cgtcggtagt agttgagata gttacgtttt gagcggtata ataattaccg
     1441 gctggtggat caaaagtcgg tgtactgacc agatcgagcg gtgcgggagc aactgcccca
     1501 gcccaggtcg tgtccacccg aatgccatca acaataaccc gctgagaagc actatattgt
     1561 cgcagggcaa cactgccgac attgtcaaaa atactggttt cattaaagat caaattgggt
     1621 tcagccggtt ctgcggttgg gaatgtatca aaaacgtata gactgcttac tttcgtagcc
     1681 aaatcatatt tcacaactaa gagtgtcggt ttacccggtg tgatagcaac ataattagat
     1741 ggcgcagtac cactactgat cgctacacca tcaccagtgg cgttcaccca tactcttcct
     1801 aaccatgtcg taccaattgt tgactgacct aagtggaaga aataccccgc cgcattcggg
     1861 cttgcggttt ggataataaa tgaggcatag actgttcccg aagtgaccgg atcaaaaact
     1921 ctgtgaacat cttctccagt attatcaaga ttagccgctc cgccaatacc agaacttagg
     1981 tagccgtcaa ataccaatcc gctggttaca tcagtagctt gacttccact accactatga
     2041 gcaacccaac cgtgattagt gaggagctca cctatttcat aatcaaaatt ttctacgaac
     2101 ggtaggctcg ggtagacttg agtcgcttga gaaagctcgg gaactattcc atctgttttg
     2161 tagtcatagt attcaccacc aacactagta ttggaaaccg gccagatttt agcgtagtaa
     2221 gtaacaccgt catccagacc agtgaaagtc acggtttgga caccgtgatt aacatatttc
     2281 acccatttat tttcaccaat atcagaatat acttgtccat ctaccggggc aaaactactg
     2341 gctgtttcca gaacgaccag aaattgttcg gccggataat caccatcgtt atccaaccag
     2401 gtcagcttga tactggtata ggtcacttcg gtggccgtaa aagaggcgac atggttggaa
     2461 ggctccgcca tcaaagtctt ggcgctacta ctaacacccg tggagtaatt caggcttcca
     2521 tctacggaaa agaatttata ataataggtc gtattcggcg tcaagccggt gtgagtgtaa
     2581 cttgtctgat ctccaccatt gtaaagcaca gccgcaccat cagctaaatg ttcaccaacg
     2641 gcataggcgg ttccattaac cggagtccca atatcgtcag tagttttata gactagcata
     2701 acattatcac cagcaccatt tttattccag gtaaagctga gcgaagttgt tgttttacca
     2761 gaaagagttg gaactggatc atctggtccg gttactccta atgtggttcc gttgaccggg
     2821 accccggcgg aataattaaa agccttgtca acagtataaa cccgatagta gtaagttgtc
     2881 tcactcgaca gattttcgtc ggtaaaagag gtcgccgtac caatataaac tacttcctct
     2941 ccggtagcaa cggtatttcc aatcgcataa attccattga cgttcggagc ggtagtcgga
     3001 tcggct
//
LOCUS       DAOOMU010000150         3001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_39939_length_3001_cov_25.432111, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000150 DAOOMU010000000
VERSION     DAOOMU010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39939_length_3001_cov_25.432111"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /locus_tag="PLI55_09655"
     CDS             <1..1047
                     /locus_tag="PLI55_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB01135.1"
                     /translation="VGTLTYWKRAHELNGNVCRYVTWYRSFGGYPEDICLNLPLIASK
                     PWFIKPRLAFIRHIDRRDPYAALPGFPPYWNPPNWQKKLFRMREILWQPIIDRAIREY
                     GLEDFDVYHFEWGADFYRDARFAKKMKSRGKKIICSYHGQDMRNRGVIPEMDKISDLN
                     LTSELDLTFRHPNLKYLFLPYDVLAFQPKNTPGKPLTICHATTNRRIKGSDEIIHVCQ
                     ELERSHGIRFIFIENQPHSEVIELKKQADIYIDQITDHAWGYGMNSLEALSLGLVCVT
                     YLNPTYENFIPDHPFVNAGISNLKEKLLELIENPANLPARMAASREWVERRHDYRNVV
                     KELYRYYESIGVRF"
     gene            1049..2107
                     /locus_tag="PLI55_09660"
     CDS             1049..2107
                     /locus_tag="PLI55_09660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPB01136.1"
                     /translation="MKITFVLPGFIKIPMGGVKVVNEYANRLAEKGHEVTLVYPLALR
                     TGNRGYALRKKISSLIDRIQRVPESLYYTPNPIVNVMAVRNTTSKYIPNGDTIIAVGW
                     QTAEAVAELPAEHGRKFYLLQSFETYFSQKNRILATYHLPLEKIAVSHWIIDELEKLG
                     EKCFGPLGNAINPTEFFIENPQPERSNDVLMIYHPHKIKGAKEGLAALQLIKRQFPTL
                     TAIIVAPRKPVHRIPSWIKVIIRPSISELRHLYNISKIFLHPSRWEGWPLPPLEAMAC
                     GCAVVATANRGVQEYLVDRENALLCPIDDVKTLAQNVITLLESDQLREELAKGGIKTV
                     QRYNWRECTSRLEQYLMG"
     gene            2178..>3001
                     /locus_tag="PLI55_09665"
     CDS             2178..>3001
                     /locus_tag="PLI55_09665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB01137.1"
                     /translation="MCLNSLLRQNYPQEKYSVIVVNDGSNDATGTLLRQMNLPENFGI
                     IEHPENCGLAAARNTGIQKSNAEIIIFLDSDMEVAPDFISRHIAWYKRPEVVGIVSEL
                     QPAPESPYDKYQRYLYESKRGARVIGANKPLPFQYFIFGITSVRWSALATVGGFNADI
                     RHYGGEDTELAYRLWQKYPTGLFFDPEIIVIHHHYRPFQQVLKIVEQFGRQVVPYIVE
                     QHPELNRLYGMQFVKPLGGNNWKLKYWIGRTLQTNVALEILWFLFEIKPYPISNFLV"
BASE COUNT          842 a          678 c          688 g          793 t
ORIGIN      
        1 gtcggcacgc ttacttactg gaaacgggca catgaattga acggcaatgt ctgccggtat
       61 gtaacctggt atcgcagttt cggcggttat ccggaagaca tttgtttgaa tctgccactg
      121 attgcgtcta aaccgtggtt tatcaaaccg cgactggctt tcattcgcca tatagatcgc
      181 cgcgatccgt atgccgcatt gcctggcttt ccaccttact ggaatccgcc gaactggcag
      241 aaaaagcttt tccgaatgcg cgaaatattg tggcaaccga ttattgaccg tgccatacgg
      301 gaatatggct tggaggattt cgatgtttac catttcgaat ggggcgcaga tttttaccgc
      361 gacgcgcgtt ttgctaaaaa aatgaagtca cgcggcaaaa aaatcatctg ctcctatcac
      421 ggtcaggata tgcgcaaccg cggcgtcatt ccggaaatgg ataaaatcag cgacttgaat
      481 ttaaccagcg aactggattt gacctttcgc catccgaatt tgaaatatct ttttttaccg
      541 tatgacgtgc tggcttttca gccgaaaaat acgcctggca aaccgctgac gatctgccat
      601 gcaaccacga atcgccgaat aaaaggctct gatgaaatca tccacgtctg ccaggaactc
      661 gaacggagtc acggtattcg ttttattttc atcgagaatc aaccacattc cgaagtgatc
      721 gagctgaaga aacaggctga tatttacatt gaccagatta ccgatcatgc ctggggctac
      781 ggtatgaatt cattggaggc actttcgctg ggattggttt gcgtgactta tttaaatccc
      841 acttacgaaa attttatacc cgatcatccg ttcgtgaatg ccggtatttc caacctgaaa
      901 gaaaaattac tggaactgat tgaaaatcca gccaatttgc cggctcggat ggcggctagc
      961 cgcgaatggg tggaaaggcg gcatgattat cggaatgtcg taaaggaatt gtatcggtat
     1021 tacgagtcca tcggagttcg attctaatat gaagataact tttgttttac ctggtttcat
     1081 taaaattccg atgggcgggg tcaaagtcgt caacgagtat gccaaccgcc tggctgaaaa
     1141 ggggcatgaa gtaactctgg tttatccgtt agctctgaga accggaaatc gaggctatgc
     1201 acttcgcaaa aaaatctcct cattaattga tcgaattcag cgcgtccccg aaagccttta
     1261 ttataccccg aatccgattg tgaacgttat ggcggtgcga aatactacat cgaaatatat
     1321 cccgaatggc gataccatta tagccgtcgg ttggcaaacg gcggaagccg tagcggagtt
     1381 accggcagaa cacggtcgca aattttattt attgcaaagt ttcgagacct atttttcgca
     1441 aaaaaatcga attctggcaa cttatcatct gcctctggaa aaaattgcag tgtcgcactg
     1501 gatcattgac gagctggaaa agcttggtga aaaatgcttt ggtccgctcg gtaatgcaat
     1561 taatcccacc gaatttttca ttgaaaaccc tcaaccagaa cgtagtaacg acgtactgat
     1621 gatttatcat ccgcataaaa tcaaaggcgc caaagaaggt ttagcggctt tgcaattaat
     1681 caagagacaa ttcccgacat tgaccgcaat aatcgttgca ccccgtaagc cggttcatcg
     1741 aatcccttcc tggattaaag taattatccg gccgtccatc agcgagttga ggcatttata
     1801 caacataagt aaaatatttc ttcatccgag tcgctgggaa ggctggcctc taccgccttt
     1861 ggaagctatg gcttgcggtt gtgcggtagt ggcaactgcc aatcgcggtg tgcaggaata
     1921 tttagttgac cgggaaaacg ctcttctatg tccaattgat gatgtcaaaa ccttagccca
     1981 aaatgtaatc accctacttg aatcagatca actgcgagag gaactggcaa aaggtggtat
     2041 taagaccgtc cagcgttata attggagaga gtgcaccagc cgactcgaac aatatttaat
     2101 ggggtaatag cctgaataat tatcaaccaa ctgtcagcgt tatcgttccg gcttataatg
     2161 ccgctaaaac tctaccaatt tgcttaaatt cattgttacg gcaaaattac ccacaagaaa
     2221 aatattcggt tatagttgtt aatgatggtt caaatgatgc taccggaact ttacttcgac
     2281 aaatgaattt gccggaaaat tttggcatca ttgaacaccc agaaaactgc ggcttagccg
     2341 ccgcgcgcaa caccggaatc caaaaatcga atgctgaaat cataatcttt ctggactccg
     2401 atatggaagt agcgccggat ttcatcagtc gtcatattgc ctggtataaa agaccggagg
     2461 tcgtaggaat agtgagtgag ttgcaaccag cgccggaaag tccatacgac aaatatcagc
     2521 gctatctgta cgaaagtaag cggggagcac gcgttatcgg cgccaataag ccactgccgt
     2581 ttcagtattt tatttttgga ataacctcag tcaggtggtc ggcactggcg actgtcggcg
     2641 gtttcaatgc tgatattcgc cactacggtg gcgaagacac cgagctggct taccgactct
     2701 ggcagaaata tccgacaggg ttgttcttcg atccggaaat tatagttatt catcaccatt
     2761 accggccctt tcagcaggtg ctgaaaatcg ttgagcagtt cggacgccaa gttgtgcctt
     2821 atattgtcga acagcatcca gaactaaacc gattgtatgg catgcaattc gtcaaaccct
     2881 tgggcggtaa caactggaag ctaaaatact ggattgggcg aactctgcaa accaacgtgg
     2941 cgttagaaat tctctggttt ctatttgaaa taaagcccta tccaatttct aattttctgg
     3001 t
//
LOCUS       DAOOMU010000151         2992 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_40116_length_2992_cov_6.427647, whole genome shotgun sequence.
ACCESSION   DAOOMU010000151 DAOOMU010000000
VERSION     DAOOMU010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40116_length_2992_cov_6.427647"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..>2992
                     /locus_tag="PLI55_09670"
     CDS             181..>2992
                     /locus_tag="PLI55_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPB01138.1"
                     /translation="MKELNYYYNKFKYGEDCYHYLMANKVKEILLVSTFYDAFIFEQD
                     GRLSEQIYGEYRQLNLSTAPMITSVPTGEDALAILKEKKFDLVITMLHIGAITPFDLA
                     RRIKEENPELPVLLLLNMLSDVKLVNIREGKLKYFDDIFLWSGDTKLFLAMVKSVEDK
                     RNLEFDTKNGLVRVILFVEDSVQFYSILLPLLYEEIIKQTQLLIDEELTDINKRLLMR
                     TRPKVILAHNYEEALRIFTDYQEYILAVISDISFSYKGKIDPEAGIKLISKVKENEYD
                     IPTILLSSEEENYNKVSQLGSFFLYKQSKHLLLHIRSFIKDNLGFGDFIFRDENGHEI
                     DRAHNLIELEEKLQIIPDESLVYHSKSNHFSSWLIARGETRIARQLRLVTVEDFETVA
                     ELRQHLITTFRTIRKARNMGKIISFSPQNLSENTLISKLSEGSLGGKGRGLAFLNALL
                     VAMDFSKKFQKVQISIPVTAIIGTNEFEDFIIRNKINELPLEEKSDGEIDQIFINGQL
                     SEELTHKLEIYLDYIHYPLAVRSSGLLEDSYAQPFAGIYRTYMLPNNSSNSKTRFEQL
                     VAAIKLIFASIYIKNARNYIESLNHRIEEEKMAVIIQQMVGSPMGNDYFMPHFSGVAQ
                     SYNFYPITGLKNEDGIASVAIGMGKLVIEGGRSFQFCPRYPHLEFIQPRALVNNSQKK
                     FLALNMKNSVYSLINGEEVTITELPLSEAEKFGILDQMASVWNMEDERLIPGINIPGP
                     RVITFENILKNETFPLANILDNLLEIGEKALGTPVEIEFAVNLTNKPQNNVFPTFYLL
                     QIRPLAVESSSALLFDEIPDHTKLLLYTEKGMGNGVIQNLYDIIYLDPEKFDNLKTLE
                     MQAEIESLNEIMKNKNLQYILIGPGRWGSQDRFLGVPVKFIQVSNARVIVEVGLENFN
                     IEPSQGTHFFHNIVAMKVGYFNI"
BASE COUNT          993 a          539 c          528 g          932 t
ORIGIN      
        1 aatttattcg cacctacctt aagttttcgt aaaatttcct cactcgcaga aaacaggcgc
       61 agaaacccaa attttctgtg cctgttggtt ttaaaggttt ggattaaatt aaaaaatatt
      121 cttaaaaaat tagaccaatg tcaatataat tttatttaaa ttaattaaga tattatgaat
      181 atgaaagaac tcaattacta ttataataaa ttcaaatatg gtgaggattg ttaccattac
      241 ctgatggcta ataaggtaaa agaaatttta ctggtttcga ctttctatga tgcctttatt
      301 ttcgaacagg atggccgttt atccgaacaa atttatggtg aataccgtca acttaatctc
      361 agcactgccc cgatgattac cagcgttccg actggtgagg atgccttggc aatattaaag
      421 gaaaagaaat ttgatttagt gataacaatg ttgcacattg gtgcaattac accattcgac
      481 ctagcccgtc gcattaaaga agaaaatcct gaactgccgg ttttgcttct attaaacatg
      541 ctatcggatg tcaaactggt caatattcgt gagggtaaat taaaatattt tgatgatatt
      601 tttctctgga gtggcgatac caaattattt ctggcgatgg taaaatcagt tgaggataag
      661 cgtaatttgg aattcgatac aaaaaatgga ttggttagag tcattctatt tgttgaggat
      721 tcggtgcagt tttattcaat tttacttcca ttattgtatg aagaaatcat taaacagaca
      781 caacttctga ttgatgaaga attaactgat ataaataagc gtcttttaat gcgtactcgg
      841 ccgaaagtca ttctagctca taattatgaa gaagccttaa gaatttttac tgattaccaa
      901 gaatatattc tagcagttat ttcagacatc agtttttcat ataaaggcaa gattgatccc
      961 gaagccggca ttaaattgat ttcaaaagtc aaagaaaacg aatatgatat tccgacgata
     1021 ctactatcat ccgaagagga aaactataat aaagttagtc agttaggttc tttcttccta
     1081 tataaacaat ccaagcacct tctacttcat attcgatcct ttatcaaaga taatcttggt
     1141 ttcggcgact ttatttttcg agatgaaaac ggtcacgaaa tagatcgggc tcataatctg
     1201 atagaattgg aagaaaaact acagatcatt cccgacgaat ctctcgttta tcatagtaaa
     1261 tcaaatcatt tctcttcctg gcttattgcc cgaggggaaa caagaatcgc tcgtcagcta
     1321 agacttgtga ctgttgagga cttcgaaact gttgcagaat tgcgtcaaca tcttatcacc
     1381 acattcagaa caatacgcaa agcccgcaat atgggtaaga ttattagctt cagccctcaa
     1441 aatctttccg aaaacactct gatttcaaaa ctcagcgaag gttcattggg tggcaaaggt
     1501 cgcggtttag catttctgaa tgctctttta gtagctatgg atttctcaaa aaaattccaa
     1561 aaagttcaaa ttagcattcc tgttacggct atcattggta caaatgaatt tgaggatttt
     1621 attattcgca acaagatcaa tgaactaccg ctagaggaaa aatctgatgg cgaaattgat
     1681 caaatattta taaatggcca actttctgaa gaacttaccc ataaattgga gatttatctt
     1741 gattacattc attacccatt agcagtacgc tcttccggtt tacttgaaga ttcttatgct
     1801 caaccgttcg caggaattta ccgaacttat atgttgccca ataactcgtc caattcaaaa
     1861 accagatttg agcagctagt ggcagctata aaattaatat ttgcctctat ttatataaaa
     1921 aatgcacgta attatattga aagtttaaat catcgaatag aagaagaaaa aatggcagtc
     1981 attatccagc aaatggtagg ttcccccatg ggtaatgatt attttatgcc gcatttttct
     2041 ggtgtcgcac aatcatataa tttctacccg attaccggct taaaaaatga agatggcatc
     2101 gctagtgtag caattggtat gggaaagtta gttatagaag gcggtcggag ctttcaattt
     2161 tgcccacgtt atccacatct tgaatttatc caaccgcgag ctttagtgaa taattcacag
     2221 aaaaaattcc tggctttaaa tatgaaaaat tccgtttaca gccttataaa tggagaagaa
     2281 gttaccatca ccgaattgcc attaagcgaa gcggaaaaat tcgggattct ggatcaaatg
     2341 gcttcagtct ggaacatgga agacgaacgg ttaatcccag ggattaatat tccaggtccg
     2401 cgagtaatca cattcgaaaa cattctaaaa aatgaaactt ttccactggc taatattcta
     2461 gacaacctgc tggaaattgg agaaaaagct ctcggtaccc ccgttgaaat cgaattcgca
     2521 gttaatctta ctaataaacc acagaataat gtctttccaa ctttctacct tttacaaatc
     2581 cgtcctttag cagttgaatc gtcaagcgct ttacttttcg atgaaatccc agatcatact
     2641 aagttattgc tttataccga aaaaggaatg ggcaatggtg ttattcaaaa tctatatgac
     2701 attatctatc tcgatcctga aaaatttgac aatctcaaaa ctttagaaat gcaagctgaa
     2761 atagaatctt tgaatgaaat aatgaaaaac aaaaacctcc aatatatctt aattggccca
     2821 ggccggtggg gttctcaaga tcgcttcctt ggcgttccgg taaaatttat ccaggttagt
     2881 aatgctagag taattgtcga ggttggtttg gaaaatttca acatcgaacc gtcacaagga
     2941 actcatttct ttcataatat tgtggcaatg aaagttggtt atttcaatat tc
//
LOCUS       DAOOMU010000152         2929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_41332_length_2929_cov_4.837161, whole genome shotgun sequence.
ACCESSION   DAOOMU010000152 DAOOMU010000000
VERSION     DAOOMU010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41332_length_2929_cov_4.837161"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="PLI55_09675"
     CDS             complement(<1..1300)
                     /locus_tag="PLI55_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01139.1"
                     /translation="MNFQNNNDPSHGFYDLKLLLIILLFFVLFISGFSQNKPLDNSQI
                     VAQIGNMTITVEDFLQRSELTVRPVYLKDKNTAINNLIAEKTLALEAEGSATEFLNKP
                     GIAGKLRGIKEQMMREALYYEVAFNKAQVDTTEINKVYRSSMREYELEFYVLKSKDLA
                     QKVQTVLDSMPELTDKMFEEFAGAIDKKPVHRINYNDNEDDVIHKALYSELLDTGTVL
                     GPLRLNDGNYIIMKVTDYIDYPLISSEDQRLRWNKVQNKLHQAAASKLWQEFQADLMH
                     GKKVEFDPNSFEVLKNLAFEMYLSSDATDSINFQIAEIVPSKPEINPNAPFFRIDDQT
                     WTIDDFKRELLAHPLIFRTRNLTKDNFAEQFKLAVVDLIRDYYLTQESYKRGLDQSPE
                     IDRTLAIWQDALIAQNQIKTVLEEAVSNGIINDDLEELAQY"
     gene            complement(1465..>2929)
                     /locus_tag="PLI55_09680"
     CDS             complement(1465..>2929)
                     /locus_tag="PLI55_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HPB01140.1"
                     /translation="QMLLGNYNGIPSRYVTPLQGIRNKVSDPTTVIYEPGCNLVEPGN
                     FIYNLSANVLSSEGQPGLKVEYFASQDLSGQPFFSRVDKIHGLNWSYGTTIPSFKGRE
                     NIASICWSGMFKAPTSGRYKFTIKSDSRVRLIIDNKIIIEDWKERKFASLSNSIKLKE
                     GEMYPLVLEFSRCGNRPQLFVEWELLNHNSFKNALDLAQKSDVVIFVGGLTAQLEGEE
                     MAVSYDGFYKGDRLSLDLPKVQEKLLKELHATGKPIVLVLTSGSALSVNWAQENLPAI
                     IQLWYPGQEGGTALADVLFGDYNPAGRLPITFYKSVEQLPPFEDYNMRGRTYRYFEGE
                     PLFPFGYGLSYTNFAYSNLIIDEEIKAGEIIKVAVDVSNRGKIAGDEVVQLYVKNLDT
                     TVPTPIHSLQGFKRINLKPGETRRVEFLLQPRQLAVIKDQDMLNSENESSRKVQYIVE
                     PGAYEISVGGMQPVTISETTQIISKKIKIIGESYLIK"
BASE COUNT          821 a          619 c          571 g          918 t
ORIGIN      
        1 gatattgagc caattcttcc aggtcatcat tgattattcc attactaacg gcttcttcta
       61 ataccgtttt gatttgattt tgagcgatta gcgcatcctg ccagatagcc agagttcgat
      121 ctatttcagg agattgatcc aatcctcgtt tgtatgactc ctgggtcaaa taataatccc
      181 gaatcaaatc aacaactgct aacttaaatt gctcggcgaa gttgtcttta gtcaggtttc
      241 tggtgcggaa tatcaatgga tgagccagta actcccgttt gaaatcatca atagtccagg
      301 tctggtcatc aattctaaaa aaaggagcat taggattgat ctccggctta gacgggacta
      361 tttccgctat ttgaaaatta atagaatcag ttgcgtcaga agacagatac atctcaaaag
      421 cgagattctt caggacttca aatgagtttg gatcgaactc gacttttttc ccatgcatta
      481 gatcggcttg aaattcttgc cacaatttgg aagccgcggc ttgatgtaac ttattctgta
      541 ccttattcca tcgtaagcgt tgatcctcac tgctgatgag gggataatca atgtaatcag
      601 ttactttcat gataatataa ttgccgtcat ttagccgaag aggaccaagc actgtgccgg
      661 tgtctaaaag ttccgagtac aatgctttat gaatgacgtc gtcctcgtta tcattgtaat
      721 tgattcgatg gacgggcttt ttgtcaattg ctcccgcaaa ttcttcgaac attttatcgg
      781 tcaactccgg cattgaatcc agaaccgttt gaactttttg ggctaaatct ttgcttttga
      841 gcacgtagaa ttcaagttcg tactcccgca tagatgagcg gtaaactttg ttaatttcag
      901 tggtatcaac ttgtgcttta ttaaaagcca cttcataata gagcgcctcg cgcatcattt
      961 gttcttttat gcctcgtaat tttccggcaa tccccggttt attcaagaac tccgtagcgg
     1021 agccctcagc ctcgagcgcc agagtttttt ccgctatcaa gttattaata gctgtattct
     1081 tatctttaag gtaaaccggg cggacggtta actcactgcg ctggagaaaa tcttctacgg
     1141 tgattgtcat attaccaatt tgagcgacta tttgggaatt atctaaaggc ttgttttgag
     1201 aaaagccgct aatgaaaaga acgaaaaata gtaagataat tagtaataat tttaaatcat
     1261 agaaaccatg agaagggtcg ttattatttt gaaaattcat catttatctt tctgttaata
     1321 gtgaaatatt tttcgaatga ccaaccaatt taaatgttaa atataaacta atttacccca
     1381 cctttgtttc agtatcatga acgtcaaagc taaattcatg gactaaattc cgcatgtttt
     1441 agtaaatcaa taaattgcac gcatttattt gatcagatat gattctccaa tgattttaat
     1501 tttctttgat attatttgag tggtttcgga aatagtgacg ggttgcattc cgcccacact
     1561 aatttcataa gcgccgggtt caacgatata ctgaaccttg cgagatgact cattttcact
     1621 attgagcata tcctgatctt taataaccgc cagttgtctg ggctgtagga gaaattccac
     1681 tcggcgagtc tcgcccggct ttaaatttat gcgtttaaac ccttgcaagg agtgaatggg
     1741 agttggtacg gtagtatcca ggtttttcac atacaattgc acgacttcat ccccagctat
     1801 tttaccacgg ttgctgacat ctacagcaac tttaatgatc tcaccggctt ttatttcttc
     1861 atcaataatg agattactat aagcgaaatt ggtgtagctc aatccatatc caaatggaaa
     1921 tagcggttca ccctcaaaat agcgatacgt gcgcccgcgc atgttatagt cctcaaaagg
     1981 cggtaattgc tccacagatt tgtagaaggt gattggcaat ctgccggccg gattataatc
     2041 accaaataat acatcagcca gagccgttcc gccttcctgg ccgggatacc aaagctggat
     2101 aatggcaggt agattttcct gcgcccaatt tacggacaaa gcactaccgc tggtcaatac
     2161 cagtactatc ggctttcccg tggcatgcag ttcttttaat aatttttcct ggactttggg
     2221 tagatccaga ctcagcctat cgcctttata aaagccgtca tagctgaccg ccatttcttc
     2281 accttctaat tgagcagtta gccctccgac aaaaatcact acatcagact tttgagctaa
     2341 atcaagagca tttttaaagg aattgtggtt cagtaactcc cattcaacga agagttgcgg
     2401 gcgattacca cagcgtgaaa attccagaac aagaggatac atttcacctt cttttaattt
     2461 gatggaatta ctcaggctgg cgaatttccg ctctttccag tcttcgatga ttattttatt
     2521 gtcgattatc aagcgcactc ggctatcgct ttttatcgtg aatttgtaac ggccggaggt
     2581 gggtgcttta aacataccgc tccagcagat cgaggcaata ttttctcgac ctttaaatga
     2641 gggtatggtc gtgccgtagc tccagttcaa accatgaatt ttatcaactc tggaaaaaaa
     2701 tggttgcccg cttaggtctt gactggcaaa atattcgact ttcaatcctg gttgaccttc
     2761 actgctcaaa acatttgcgg ataaattata gataaaatta ccaggttcaa ccaagttaca
     2821 accgggttcg taaattacag tcgtcggatc ggaaacctta tttctaatcc cttgtaaagg
     2881 agttacatac cgggaaggga taccattata attgcccagt aacatctga
//
LOCUS       DAOOMU010000153         2929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_41334_length_2929_cov_4.251218, whole genome shotgun sequence.
ACCESSION   DAOOMU010000153 DAOOMU010000000
VERSION     DAOOMU010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41334_length_2929_cov_4.251218"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2699)
                     /locus_tag="PLI55_09685"
     CDS             complement(<1..2699)
                     /locus_tag="PLI55_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB01141.1"
                     /translation="MKDRVIFGIWLLLISILLNGILFGGTTGKITGVITDASTSAPII
                     GANVILKGTTMGAASDSEGNFTILNVPPGTYTVVISMIGYQQIQFEDVRVSIDLTTRL
                     DATLKQTALDLGESVIVTAERKLVLRDMTSSLSTIDADQISNLPVQQIQDVLRLNAGI
                     IESDGRLHIRGGRAGEVAYWVDGISATDVYDGRIGINIENSAVQELQVISGTFNAEYG
                     QAMSGIVNIITKEGGQDYTGYIKVYGGDYVSDDNVFGLYKRILYDSTRSNILSKEWEN
                     PLEKFNPIYNGEFSLGGPVPLFKDNLSFFINGRYYYDEGYYYGRTWYQPNGAPIDSTV
                     LTSQMKRLYATNGITFGGSSLIPMNPMERTTFQGKLSARLSRSMKLNYNFFWNESYQE
                     RAYFRTNTNDYQFNPTGTANYQQFNTHNYKYNPYGLPQIHNQGMAHTLTLNHIINPSN
                     FYELRVSRYESKSKQYVFDNPNASNDYLVYVKEDTSKDIIAEIFDPFTERGAIKFDSL
                     KYLGAQYEYIVNPEGSVGYIDPDDITAPTSYSFMNSGMDVTQANRKTAYWVGKLDFTS
                     QVNKYNQLKFGSEVRLYELTLHSYQIVAATNESGQEITPFQPAIPEVGSIYRNDYFRE
                     PKEFSAYIQDKLERNNIILNVGIRYDYFDANSVILSDPTDPNIYSPFKYSNKYAGWID
                     VPNNYRGTIDQWVDSLLASGIIREYTPDERRAFMHKKVKAKSSWSPRLGLAFPITDRG
                     VIHISYGHFFQIPEFQYLYTNPDFKITGGSGTALFGNPDLKPQKTVMYEIGLQQQFGE
                     NIGIDLTLFYRDVRDWVGTSPIIPTVKTGVSYSQFVNKDYSNVRGVTFKIEKRFSQNY
                     SFRADYTYQSAEGTYSDPTDAFNSALNNEQPVLALVPMEWDQR"
BASE COUNT          904 a          602 c          502 g          921 t
ORIGIN      
        1 tgcctttgat cccactccat tggcactaac gctaagactg gctgttcgtt atttaaagcc
       61 gaattgaagg cgtcggtggg atcagaatag gttccttcgg ctgattgata agtataatca
      121 gctctaaagg aataattttg actaaatcgt ttttctattt tgaaggttac tccccgaaca
      181 ttggaataat ccttattcac aaattgagaa taagaaacgc ctgtttttac cgtcggaatt
      241 atcggacttg taccaaccca atcgcgtaca tcacgataaa ataaagtcag atcaatccct
      301 atattctcac cgaactgttg ctgtaatcca atttcataca ttactgtttt ttgaggctta
      361 agatcaggat ttccaaaaag agcagtcccc gagccacctg taatcttaaa atcaggattg
      421 gtataaagat attggaattc aggaatttgg aaaaagtgac cataggaaat atgaatcacg
      481 ccccggtcag taatcgggaa tgccaatcct aaacgagggc tccaggagct cttggctttt
      541 acctttttat gcataaaagc ccgtcgttca tccggagtat attctctaat tattccacta
      601 gctaaaagag aatcaaccca ttgatcaata gttccacggt aattatttgg cacatcaatc
      661 caaccagcgt atttatttga gtatttaaac ggagaataaa tattggggtc ggtcggatca
      721 gataatataa ccgaattagc atcaaaataa tcatacctga ttccgacatt gagaattata
      781 ttatttcttt ccaatttgtc ttgaatatag gcggaaaatt ccttcggctc cctgaagtaa
      841 tcgttgcgat agattgaacc gacttctggg atagccggtt ggaatggcgt aatttcctga
      901 ccactttcat tagttgccgc cacaatctga taactatgta aagtcagttc atataaccga
      961 acctccgatc caaatttaag ttgattatac ttatttacct gactagtgaa gtccaatttc
     1021 ccgacccagt aggctgtttt ccgattagct tgggtaacat ccatcccgct attcataaaa
     1081 ctataagaag taggagcagt aatatcatcc ggatcaatat atccgaccga gccttctgga
     1141 tttacaatgt actcgtactg agcgcctaaa tactttagtg aatcaaattt aattgcaccc
     1201 cgctcagtaa aaggatcaaa tatttctgct ataatatctt tacttgtatc ctctttaacg
     1261 taaactaaat aatcattaga ggcattggga ttatcaaaaa cgtattgttt tgatttcgat
     1321 tcatagcggc tgactcgcag ttcatagaag ttcgaggggt tgattatatg atttaacgtt
     1381 aatgtatgag ccattccttg attatgaatt tggggcaaac catagggatt atatttgtag
     1441 ttatgagtat taaattgctg atagtttgca gttccagttg gattaaattg ataatcgttg
     1501 gtattcgttc ggaaataagc tcgttcttga taggattcat tccagaaaaa attataattt
     1561 agcttcatcg aacgacttaa acgggcactt aatttccctt gaaacgttgt tctctccata
     1621 ggattcatcg ggataagtga gctcccgcca aatgtgattc cattggtggc atacaatctt
     1681 ttcatttgcg aagtaagaac tgtactatct atcggggcgc cattaggttg ataccacgta
     1741 cgtccgtaat aataaccctc gtcgtagtaa tagcgtccat taatgaaaaa acttagatta
     1801 tctttaaata aaggtactgg cccacctaaa ctgaactcac cattatagat gggattgaat
     1861 ttttctagcg gattttccca ttccttggac aatatattcg agcgtgttga atcgtataaa
     1921 atcctcttgt ataaaccgaa aacattatca tcacttacat aatctccacc ataaacttta
     1981 atatatccag tataatcctg cccgccctct ttcgtgatga tatttactat acccgacatt
     2041 gcctgtccat attcggcatt gaaagtacca cttataacct gtaactcctg tacggcggaa
     2101 ttttcgatgt tgatgccaat tcgcccgtca tatacatcgg tggctgaaat gccatcaacc
     2161 caatacgcta cttctccagc ccgaccacct cggatatgca atctaccgtc agattcgata
     2221 ataccggcgt ttaatctcag aacgtcctgg atttgctgaa ccggcaaatt cgaaatttga
     2281 tcggcatcta tggtagaaag cgaagaagtc atatctctaa gaaccaattt cctttcggca
     2341 gtcacgatca ccgattctcc taaatctaaa gccgtctgct ttaaagttgc atccaatcga
     2401 gtagtaagat caatacttac tcgaacatct tcaaactgaa tctgttgata accaatcata
     2461 ctaattacca ctgtgtatgt gccaggtgga acatttaaaa tggtaaaatt accctcgcta
     2521 tcacttgccg ctcccatagt agtgccttta agtataacgt ttgcaccgat aatcggagcc
     2581 gaagtactgg catcggttat cactccggtt attttaccag tagtcccgcc aaataatata
     2641 ccattaagta aaatactaat taggagtaac catatcccaa atattactct gtctttcatc
     2701 acagccgcct ttatttttag agtgagtaaa attgtcttca taaaaaaatt cactataact
     2761 aaaatattaa attacagagc aaattaatag caagtattta atttaaaaag agatagacac
     2821 tgaactccga aacttcaatt gatatattta ttgtcatcat cattgcttat acaatttttc
     2881 ttcatcattt gtgaaaaaat tttacatttt tagtcttaat aatacatag
//
LOCUS       DAOOMU010000154         2898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_41944_length_2898_cov_7.930355, whole genome shotgun sequence.
ACCESSION   DAOOMU010000154 DAOOMU010000000
VERSION     DAOOMU010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41944_length_2898_cov_7.930355"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /gene="cas6"
                     /locus_tag="PLI55_09690"
     CDS             <1..893
                     /gene="cas6"
                     /locus_tag="PLI55_09690"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HPB01142.1"
                     /translation="KFNRFPSATLRGAFGTSLKSTLCIHSAINANCEKCLIKKTCAYY
                     YLFETGTDLHGENNPHPFVILLPLELANRTILPGEIFSFHLVLFGYGLDYLPHIIYAF
                     ENLGKKGLGSRKGKFDLVEVKFVNQEAEKIIYFESDKILNNEDQNYLFEFHNDNGVVN
                     QIKINLLTPLRMKNEGRLVTEVNFKIFIINLLRRISKIAEIHCGEEIAIDWSELLKIG
                     EETKIISDNTYWKEYKRFSSRQKQEMLLGGLLGNFEVQGKFRELLPYLYLGEIIHVGK
                     STSFGFGKYQMQIIKKEESL"
     gene            890..>2898
                     /gene="cas10"
                     /locus_tag="PLI55_09695"
     CDS             890..>2898
                     /gene="cas10"
                     /locus_tag="PLI55_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HPB01143.1"
                     /translation="MMNDKIRESIILGALLHDIGKFWQRCEEKGGIDNASFLDSNVRN
                     TISNDICPGRKYGDVTILTHQHVAWTYQFLTKFESKIYPTGTSDEESILQFASGHHRS
                     IPSTSLQTLIQVADWISAAQDRNGDTGDIKDELSRSYNYRKQRQRPVFENLFRDDYDS
                     RGSTYRYNIGELKLDEILMPQDFKNEDRKLSSEYQKLWENFVAEFINIPNKDFNAYLE
                     TLLCLLQKYTWCIPSSTNDSPDISLYDHSRSTASIATCLYDYLQETNPSLIQPGEKTQ
                     QQKTKAFFTNNEKFALLITADISGIQKFIYQISSVKAATALKGRSFYLQLISDAAARY
                     FLTQLNLPYTNLIYSSGGNFFLLAPNTKKVQLEIARTTEQINRSLLAKYDSSLFLGVG
                     YEELSGSDFIHNLSEKWEKAIRKANSQKRRKFRDSVESDWYFFQPRPKNNAKPCDNCG
                     ADCNEAELSKIKEEEEEKYCPLCREFIQAGTRLKSAQILLEFVTSEANELDYNEIIYP
                     ITGVPVGYILTGSTQLDQNAGIISQAIFKKIYYLNQHDFIGKLSGERFTTFAWGFKFY
                     GGNHLPLNDQGEPSTFDYLAGVRENKAKETISFKRLGILRMDVDNLGKIFSKGFLLNS
                     LHNPKISNESVKAPYSISRISTLSSMLDIFFCGYLNTLHQQNEDF"
BASE COUNT          981 a          517 c          527 g          873 t
ORIGIN      
        1 ttaaatttaa tagatttccg tcggcgacct tacgaggagc ctttggaacc agtttaaaat
       61 ctaccttatg tatccattca gcgattaatg ccaattgtga gaaatgtctt atcaagaaaa
      121 cctgtgccta ttactacctt tttgaaaccg gtacggattt acatggagaa aataatcctc
      181 acccttttgt aattttacta ccgctagagc tagccaatag aaccatatta cctggagaaa
      241 tattttcatt tcatttagtt ttatttggat acggtctgga ttatcttcct catataatct
      301 atgcttttga aaatctggga aaaaagggac tgggaagtag aaagggcaaa tttgatttag
      361 tagaggtaaa atttgttaat caggaagccg aaaaaattat ctatttcgag tcagataaaa
      421 ttctaaacaa tgaagaccaa aactatctat tcgaatttca taatgataat ggagtagtta
      481 atcaaataaa aataaatttg ctaactccgc tacgaatgaa aaatgaaggg agactagtaa
      541 ctgaagtcaa tttcaaaata ttcataatca atctactgcg tcgaatctca aaaatagccg
      601 agattcattg tggtgaagaa atagcaattg attggtccga gttacttaaa attggagagg
      661 aaactaaaat aatatcagat aacacttatt ggaaagaata caaacgcttc tctagtcgtc
      721 aaaaacagga aatgttatta ggcggcttac tcggaaattt tgaagttcag ggcaaattta
      781 gagagctttt gccgtattta tatcttggag aaataatcca cgtcggtaaa agcactagtt
      841 ttggctttgg aaaatatcaa atgcaaataa taaaaaagga agaatcttta tgatgaatga
      901 caaaattcgc gaaagtatca ttctcggagc attgcttcac gatattggga aattctggca
      961 aagatgtgaa gaaaaaggtg gaatagataa tgctagtttt cttgactcga atgtaagaaa
     1021 tacgattagt aatgatattt gccccggtcg gaaatatgga gatgttacaa tattgactca
     1081 ccagcatgtg gcctggacat atcaattcct tacaaaattt gaatcaaaaa tatatcccac
     1141 tgggacttct gatgaagaat ctattttgca atttgcttcc ggtcaccata gatcaatacc
     1201 ttctacttcc cttcaaactc taattcaagt tgccgattgg atttcagccg ctcaagatcg
     1261 taatggagat actggagata tcaaagatga actaagccgt agttacaact atcggaaaca
     1321 gcggcaacgt ccagttttcg agaatctttt tagagatgac tatgatagca gaggaagtac
     1381 ttatcgctat aatatcggag agcttaaact cgatgaaatc cttatgccac aagattttaa
     1441 aaatgaagat cgaaaattat cttccgagta ccaaaaacta tgggaaaatt tcgtagctga
     1501 atttataaat attcctaata aggattttaa cgcttaccta gaaactttgc tctgtctttt
     1561 gcagaaatat acctggtgta tcccaagttc taccaacgat agtcccgaca tttcacttta
     1621 tgatcattct cgttcgacag catctattgc tacctgtcta tatgattatc tacaagagac
     1681 taatccatct ttaatccagc cgggagagaa aactcaacag caaaaaacca aagccttctt
     1741 tactaataat gagaaatttg ctttattaat tacagcagat attagcggta ttcaaaaatt
     1801 tatttaccag atttcatcgg taaaagccgc tactgcctta aaagggcgat ccttttattt
     1861 acagctgata agcgatgccg ccgcacgcta ttttcttacc caattgaatc ttccttatac
     1921 aaacttaatt tattctagtg gaggtaattt ctttttattg gctcctaata caaaaaaggt
     1981 tcaattggaa atcgccagaa ctaccgagca aataaaccgt agtctcttag ctaaatacga
     2041 tagcagtttg ttcctcggcg tgggttatga agaattatca ggttcagatt ttattcataa
     2101 tctatcggaa aaatgggaaa aggcaatccg aaaagctaac tctcaaaaac gccgtaaatt
     2161 tcgcgattca gttgaaagcg attggtattt ttttcaaccg agaccaaaga ataatgccaa
     2221 accatgcgat aactgcggcg ctgattgcaa tgaagccgaa ctaagtaaaa ttaaagaaga
     2281 ggaagaagaa aagtactgcc cgctttgtcg agaattcatc caagccggaa cccgactgaa
     2341 atcagcccaa attttactcg agtttgtcac ttctgaggca aacgagctag attataacga
     2401 gattatttat cctataaccg gagtgccggt tggttatatt ttaaccggta gcacgcaact
     2461 cgatcaaaat gccggaatca ttagtcaggc tatttttaag aagatatatt atttaaatca
     2521 acacgatttt ataggaaaat tatcgggaga acgatttact acctttgcct ggggattcaa
     2581 attctatggt ggcaatcatt tgccgctcaa tgatcaggga gaaccttcta cttttgatta
     2641 tttagccggc gtacgagaaa ataaggcaaa agaaactatc tctttcaagc ggctcgggat
     2701 tttacgtatg gatgtagata atctgggaaa aatctttagt aagggctttc tattaaattc
     2761 gctccataat cctaaaattt caaatgaatc agttaaagcg ccttattcga tttctcgaat
     2821 ttctaccctc agttctatgc tcgatatttt cttttgcggc tatctgaata ctttgcatca
     2881 gcaaaatgaa gattttaa
//
LOCUS       DAOOMU010000155         2752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_45118_length_2752_cov_19.639229, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000155 DAOOMU010000000
VERSION     DAOOMU010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45118_length_2752_cov_19.639229"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2652
                     /locus_tag="PLI55_09700"
     CDS             <1..2652
                     /locus_tag="PLI55_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HPB01144.1"
                     /translation="AECYALNDKAVESELILREALRRAPTNAMIYLHLIEAYLRGEKY
                     DEAKSAYEKIYQLDPDIPGVLEYRISELLAKKNYDEAKNLLDRLAQLIPDSPNLQLQL
                     IAYYSKRGLTEKVIEITNAAYHQFPDNWNIVETKANLEAYVKQNNKAAIPILRKYVKN
                     HTEQTAFLKLADYYKKAADLKNWEKTYRQAIDYYPASPGLFYQMAQNFYNRHNYSTAL
                     DYIEKALQICPQCAGYWGTRAEIQRSSGQSAAAIKSYQRALELSPTYYDARDKLRQLQ
                     GRASIFNNFDTVDIDSLFRNCPSSEQYPGEKAVILYNACRRVVYGGATEMSQEILATV
                     FNLEGIDAFKEYRISYNAYNEELIVEKAQTLKKDGSKIDADVSWGYIVFKSLEPGDAV
                     YLKWKIRNYYSGKLSQHFWDDFSFNTFFPVREVCYALLIPPNVKFQYKGQFMPDEPSA
                     IRETPDGILYEWRLNDVPAIKEEYNMPPLADIGKILHISSIPDWDYIVNWYIDLAETR
                     TRSCYEIDEFLKTLLPENEPLTEHEKITRIYNYITENIRYSSVSFRQSGLIPQKARDV
                     FVTRIGDCKDKAALGIAMLRSAGFTANFVLVNARYEGLNKHVLPSIAFNHAIVSVATQ
                     ERTLYLDLTAQNFPVGSVPLADQGAFALEIKKGCQPFYLTRHFFMGCVRQRRTKVEVQ
                     TDNSIQVELHTTRKGAQTAEFRAVYRFKSQADRDKELIESLAQDFPNVELISFNLFDI
                     DSIKPTLDYEYTFMVPDYLTEAGKFLILRIPWADRLSPIRALSYDKRNYPFTYWPNAD
                     TISEEITITFPAGYQPLEFTPYYEYSCPIADYRLTLDYNDRILKGARLMINKESEITP
                     EIYPAFKEFYNKVVKADGYQILIKRLE"
BASE COUNT          787 a          614 c          585 g          766 t
ORIGIN      
        1 gcggaatgct atgctttaaa cgataaagca gtcgagtccg aattgatttt acgcgaggct
       61 ctgcgacgcg ctccgactaa tgctatgatt tatttacatt tgatcgaggc ttacttgcgc
      121 ggcgaaaaat acgacgaagc caagtccgct tatgaaaaaa tttaccagct tgatccggat
      181 attccaggcg ttttagaata ccgaatctct gagctcctcg caaagaaaaa ttatgatgaa
      241 gccaagaatt tactcgaccg actggcgcaa ttgataccgg attcgcccaa tctacaattg
      301 caactcatcg cttattattc caaacgcggt ctgaccgaga aggtaatcga gataaccaat
      361 gccgcttacc atcaattccc ggataactgg aacattgtcg aaaccaaagc caatctcgaa
      421 gcttacgtta aacaaaataa caaagccgct attcccattt tacgtaaata cgtgaaaaac
      481 cacaccgagc agacggcttt tctcaaatta gccgattatt ataaaaaagc cgctgacctt
      541 aagaactggg aaaaaaccta tcgacaggcg attgattatt atccggcttc accgggcctt
      601 ttctaccaaa tggcgcaaaa tttctataat aggcataatt attccaccgc tttggattat
      661 atcgagaagg ctctccaaat ctgtccgcaa tgtgccggct actggggcac tcgcgccgaa
      721 atccaacgtt cgtccggtca atccgccgcg gctattaagt cctaccagcg agcgctggaa
      781 ttgagtccga cttattatga tgcgcgcgat aaattaaggc aattgcaagg acgcgcatca
      841 atttttaaca atttcgatac ggtggatatt gatagtcttt ttcgcaattg cccatcgtcg
      901 gagcagtatc ccggtgaaaa ggcggttatt ctatacaatg cctgtcggcg cgtcgtttat
      961 ggtggcgcaa ctgaaatgag ccaggaaatt ctggcgacgg tttttaatct ggaaggaatc
     1021 gatgctttca aagaataccg gatttcttat aacgcttata acgaagaact gattgtcgaa
     1081 aaggcgcaaa ctttaaaaaa ggatggctcc aaaattgatg ctgacgttag ctggggctat
     1141 attgtattta aatcactcga acccggtgac gccgtttacc ttaaatggaa aattcgcaat
     1201 tattactccg gcaaactatc ccagcatttc tgggacgatt tcagtttcaa tacatttttt
     1261 cctgtgcggg aagtttgcta tgccttattg attccgccga atgtgaaatt tcagtataaa
     1321 ggtcaattta tgccggatga accttcggct atccgggaaa cgccggatgg aattctctat
     1381 gaatggcgcc tgaacgacgt tcccgccata aaagaggaat acaatatgcc tcccctagca
     1441 gatatcggaa aaatattgca tatttccagc attccggact gggattacat cgtgaactgg
     1501 tatattgatc tggcagaaac cagaactcgt tcatgttatg agattgacga atttctcaag
     1561 actttattac cggaaaatga accgctgacc gagcatgaga aaattacccg tatttataat
     1621 tatatcaccg aaaatattcg ctatagcagt gtatccttcc gccagtccgg tttgattcct
     1681 caaaaagccc gcgatgtatt cgtaacccgc atcggtgatt gcaaggataa agccgccctc
     1741 ggaatcgcta tgttgcgctc agcgggattc acagccaatt tcgtgttggt aaacgctcgc
     1801 tatgaaggtt tgaataaaca cgtattgcct tcgatagcct ttaatcatgc catcgtcagc
     1861 gttgccactc aagagagaac gctctatctc gatttgacag cgcagaattt tccggtcggt
     1921 tcggtgccct tggcggatca aggggctttt gcgctcgaaa tcaagaaggg ttgccaaccg
     1981 ttttatctaa cgcgccactt ttttatgggg tgtgttcggc aacgacgtac caaagtcgag
     2041 gttcaaaccg ataatagtat tcaggtcgag cttcacacca ctcgcaaagg tgctcagacg
     2101 gctgaatttc gtgcggttta tcgattcaag agccaagccg atcgcgacaa agaattaatc
     2161 gaatcgttgg ctcaagattt tcccaacgtg gaactgatta gctttaattt atttgacatc
     2221 gactcaatta aaccgacttt ggattatgaa tacactttta tggttccaga ttatttaacg
     2281 gaagcaggaa aatttctcat tttacgcatc ccctgggctg atcggctttc gccaattagg
     2341 gcgctatcgt atgataaaag aaattatccg tttacttact ggccaaatgc cgatacgatt
     2401 agcgaggaaa tcacaatcac cttcccggct gggtatcaac cgttggaatt tactccgtat
     2461 tacgaatatt cctgtccgat tgccgattat cggcttacgc tggattataa tgatagaatt
     2521 ttaaaaggcg ctcggctaat gataaataag gaatccgaga ttacgccgga aatttatcct
     2581 gcattcaaag aattttataa taaagtcgtc aaagccgatg gctatcagat tctgataaaa
     2641 cgtctggaat agactatttt gtctcaggct tctttttatc gttatcgtct ttacagtaat
     2701 ttggatataa ttttttcata caatccgggc aaatgccgtg ggtaaattcc ac
//
LOCUS       DAOOMU010000156         2723 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_45765_length_2723_cov_22.983508, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000156 DAOOMU010000000
VERSION     DAOOMU010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45765_length_2723_cov_22.983508"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            62..2047
                     /locus_tag="PLI55_09705"
     CDS             62..2047
                     /locus_tag="PLI55_09705"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPB01145.1"
                     /translation="MMAPTNTPLGSALFIVLLVVALSWFVWLMYRRIAVIAAGQPQNR
                     FDHIGRRIGAFFTQGIAQTKIRDRRFWSAGYMHAFIFWGFLVVSINTIQMIGQGFNPN
                     FVLPGFHTGQTLGQIYLWLKDVFEVLVLLSVIYALIQRLIIRPKRLTLSASANLILIM
                     IAILMVTDLLMTGLTVTFEADADRTVVGVAFARIFRNTSPEFTVKFFHLNWWLHLITL
                     LSFFPFLPLSKHFHIITSLPAVFFKRLDSGYVRFLDVENGERFGASRIEHLGWKDLLD
                     VFTCTECGRCQTVCPAFESGKPLSPKEFNIALRHHLIKYTTDIIKDKRQKSQSPRAEK
                     LVGDTIREETLWACTTCRACEEVCPLSIEFIDRIVEMRRHLVLEESRFPEAAELAFRN
                     LESYGNPWGASDREKWTEGLAIPRAEEKADFEYLFWVGCAGAFDEHAQKTSRAMIKLL
                     NAARVNYAILGNAETCTGDAARRLGNEYLFQTLANQNIETFHNLGVQKIITQCAHCYN
                     TLKNEYPQLGGKFEVISHIEMIANLLAEGRLKINQPLKGKLTYHDACYLGRHNGIYDA
                     PRQIIAALQADFQEATRHGSNALCCGAGGGRMWLEEAPAQRVNTLRAKELTTLNPDII
                     ATACPFCTTMLYDGLKEQNSSVKCLDLAVLVASQLAE"
     gene            2104..>2723
                     /locus_tag="PLI55_09710"
     CDS             2104..>2723
                     /locus_tag="PLI55_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPB01146.1"
                     /translation="MIPFLLDERYYRYGRYLRERFQTRVYRITLDAGFTCPTRDGTKG
                     TSGCLYCNNESFAPKRAEELPSLSDQIQKGIASARRFHQADKFLAYFQPFTNTYAPVA
                     KLERLYHEVLAFSEIVGICIGTRPDCVSEEILQLLEHLNHETYISIEFGIESVYDKTL
                     DWAQRGHDFAQTRWAIQAAKAHGLHVAGHLILGFPTETRAEILATPG"
BASE COUNT          686 a          645 c          647 g          745 t
ORIGIN      
        1 tgattttgga atcaagctgg aaatgtttaa aatttcacct taaaaaagga gaaactggta
       61 aatgatggca ccaacaaata ccccactcgg atcggcactt tttatagttc tgctggtagt
      121 cgcactcagc tggttcgttt ggctgatgta tcggagaatc gccgtaattg cggctggtca
      181 gccccaaaat cgctttgacc atatcggacg acgaatcggc gcttttttca cacaaggaat
      241 tgctcaaacg aaaatccgtg atcggcgttt ctggtcggct ggctatatgc atgcatttat
      301 tttttggggc tttctggtag tttcgatcaa tacgattcaa atgattgggc agggattcaa
      361 tcccaatttc gtcctgccgg gttttcatac tggacagact ctcggacaga tttatctctg
      421 gctcaaagat gttttcgaag ttttagttct actcagcgtg atttatgccc ttattcagcg
      481 cctgatcatt cgcccaaaac gcctgacatt atccgcttca gcgaatctga ttttgataat
      541 gattgcgatt ttgatggtta cagacctttt aatgaccggg ttgaccgtca cgtttgaagc
      601 ggatgccgat cggacggtcg tcggggtagc ctttgcccgt attttccgca ataccagtcc
      661 ggagttcacc gtaaaatttt ttcatcttaa ttggtggttg catcttataa ctctgttgtc
      721 ctttttccca tttctgccgc tttccaagca ctttcatatt ataacgtcct tgccagcggt
      781 ttttttcaaa cggcttgaca gtggatatgt gcgctttttg gatgtagaaa acggcgagcg
      841 cttcggggcg tcccgcattg aacatctcgg ctggaaagat ttgctggatg tttttacctg
      901 tacagaatgc ggtcggtgtc agacggtttg cccggcgttc gaatccggca agccactgag
      961 tccgaaggaa ttcaacattg ctttgcggca tcatcttatc aagtacacga ctgatattat
     1021 taaagataaa agacaaaaat ctcaatcgcc acgcgctgaa aaattagtgg gcgacaccat
     1081 cagagaggaa acgctttggg cttgtaccac ctgccgggct tgtgaagaag tctgtccact
     1141 gagcatcgag ttcattgatc ggattgtcga aatgcgccgt cacctggtac tggaagaaag
     1201 ccgtttcccg gaagcggcag aattggcatt ccgtaatttg gaaagctacg gtaatccctg
     1261 gggcgcctct gatcgcgaaa agtggacaga aggtctggca attccgcggg cagaggaaaa
     1321 agccgacttt gaatatctct tttgggttgg atgcgccgga gctttcgatg aacacgccca
     1381 aaaaacttcc cgcgcgatga taaaattgct gaacgccgct cgggtcaatt atgccattct
     1441 gggcaatgcc gaaacttgta ccggtgacgc cgctcgccga cttggtaacg aatacctttt
     1501 ccagacttta gccaatcaaa acatcgagac tttccacaat ctcggcgtcc aaaaaattat
     1561 cacccaatgc gcccattgct acaatactct gaaaaatgaa tatccccagc taggtggcaa
     1621 attcgaggtt atcagtcata tcgaaatgat tgccaatcta ttagcggagg gacgtttgaa
     1681 aatcaatcag ccgttaaaag gtaaattgac ctatcacgac gcctgttatc tcggacggca
     1741 caatggaatt tatgatgcgc cgcgtcagat aatcgcggca ttacaagctg attttcaaga
     1801 agcgactcgc cacggtagta atgctctatg ttgcggcgcc ggcggtggcc ggatgtggct
     1861 ggaagaagcc ccagctcagc gcgtcaatac cctgcgggca aaggaactca ccacattgaa
     1921 tccggatatt attgccaccg cctgtccttt ctgcacgact atgctttatg acggattaaa
     1981 ggaacaaaat tcgagcgtaa aatgcctcga tctggcggtg ctggtcgcca gtcaattagc
     2041 tgaataaccg gtgaattttg atttgaaatt aaagattctt aaatttgaaa ttttaattaa
     2101 aaagtgattc cctttctgct ggatgaacgc tattatcgtt atggtcgcta cctacgcgag
     2161 cgctttcaga cgcgggttta tcggattacg ctcgatgctg gttttacgtg cccaacccgt
     2221 gacggcacta aaggcacttc cggctgtctg tattgcaata acgaaagctt cgccccaaag
     2281 cgtgctgaag aattgccgtc gcttagcgat caaatccaaa aaggcatagc gtcggctcgc
     2341 cgttttcacc aggccgataa atttctggct tattttcaac cgtttaccaa tacttatgcg
     2401 ccggtcgcaa agctggaaag actttaccac gaggttttag ctttttctga aattgtagga
     2461 atctgcatcg gtacgcgccc ggactgcgtt tcggaggaaa tattacaact actcgaacac
     2521 ctgaatcacg aaacttacat ttccattgag ttcggtattg aatccgtcta tgataaaacg
     2581 ctggattggg cgcagcgcgg acacgatttt gctcaaactc gctgggcgat tcaggcggcg
     2641 aaagcccacg gtctccacgt tgctggacac ctaattctcg gatttccgac tgaaacgcgc
     2701 gctgagattt tagctactcc cgg
//
LOCUS       DAOOMU010000157         2637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_47837_length_2637_cov_6.857475, whole genome shotgun sequence.
ACCESSION   DAOOMU010000157 DAOOMU010000000
VERSION     DAOOMU010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47837_length_2637_cov_6.857475"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            417..1925
                     /locus_tag="PLI55_09715"
     CDS             417..1925
                     /locus_tag="PLI55_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPB01147.1"
                     /translation="MILSVSTSLFAEKGFRLLTTISGEKSGDQFCTVKNVGDVNGDGF
                     NDLLVGAAEASYTKLYYGGSDFDTIADLTMRVPKHYDYFGQTLAAGDLNNDGFSDFAI
                     GAPYYDEYNRCGKIFVYFGGTNIDSTADMEIEGYGYWHLMGSRIVCDIDVNGDGYQDL
                     LTCSGQYPRKTYIYFGGANMDIVPDICLSGNDSIICNVGGPSGIGDINNDGYDDFFVS
                     VTSDVGLPNYLPGTGNYLIYGGENLSLENSVIFQIDSTLEWTNKKGSIGDLDRDGYND
                     FYISYWYNRVYFYSGSSLCVLDSINIDGFISSTCADFDIDEDGNNDLMCGFTNFNNQY
                     RGYVYIFTSVIGNYDTTPKYVLSGESELEYFGQSLDFLGDINNDGLPEIAIGKRSGNG
                     KVYIYSFGDFQDRIDDRYGVKSDEFTVLKAYPNPFNNSTVIEYCLKAKSQVRIDIYDL
                     SGQKVITLSNEVRQPGINRVVFNSHNYVSGIYICRITINDIQEETMKILLLK"
     gene            1957..2553
                     /locus_tag="PLI55_09720"
     CDS             1957..2553
                     /locus_tag="PLI55_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01148.1"
                     /translation="MKTYFLKKPLLAVIVLFLTGCMTTAYISSLTDTRYAPVKTDPIF
                     ILIADNASITDRQFYTFLKNEMILNGFNIVDEAPIARYFLLFQTDSKTSQINSTLFLP
                     STSTTSGYIGNTYYSGSTKSTSAVPYSYNYTVKKIYLDLYAVEDVKNEKYITVWEGYI
                     GAGQKEYQTYSRAIIKSLLDKFGTNYEAHTPIDIYYAK"
BASE COUNT          847 a          416 c          527 g          847 t
ORIGIN      
        1 attcggaagt aatttaagtt ttaatacaac tcaaaacata tcaaaaaata atggaggcca
       61 accttcagaa ggctttttgt gtagatattc aggcttttgc tagtggatac taagctctga
      121 tattttataa aatttttgaa tatttatatt gattttaccc ccccatgcgt atatattacg
      181 cctgtaaatg gaaggcattt aaaaatatgg cgcatgcgca ggtttttatc gattggtttt
      241 accggacagg gaagataagg acggagaatg cagataggaa aaacatagcc tcggaagttc
      301 cggatcgttt taaaacaagg ctaataatat agcggcagac ttattgggaa gggggcgggc
      361 tgaaaacaaa taaggataaa tctcaacaaa aatcaaaaag aattgctgct cttttagtga
      421 ttctatctgt atcaacatca cttttcgccg agaaaggctt ccgtctgctg accactatca
      481 gcggtgagaa gtcgggggat caattctgta ccgtgaaaaa tgtcggcgac gtcaacggtg
      541 acggttttaa cgatttgctg gttggagccg ccgaagccag ttataccaaa ttgtattatg
      601 gtggttccga tttcgatacc attgctgatc tgacaatgcg ggtacccaaa cattacgatt
      661 atttcggaca aaccctcgcg gctggcgact tgaacaatga tggtttttct gattttgcga
      721 tcggagcccc ctattatgac gaatataatc gttgtggaaa aatatttgtc tatttcggtg
      781 gaacaaatat tgattcaact gccgatatgg aaatagaagg ttatgggtat tggcatttaa
      841 tggggtctcg gattgtttgc gatattgatg ttaatggtga tggttaccag gatttgctca
      901 catgttccgg gcaatacccg cggaaaacct atatctattt tggaggcgct aatatggata
      961 ttgttccgga tatatgtctt tcagggaatg attcaattat atgtaatgtg ggaggaccat
     1021 ccggaattgg tgatatcaat aatgacggat atgacgattt ctttgttagc gtaacgtccg
     1081 atgttggact accaaattat cttcctggaa ccggcaatta cttaatttat ggtggagaaa
     1141 atttatctct tgaaaattcc gttatttttc agattgattc aactttagaa tggacgaaca
     1201 aaaaaggtag cattggcgat cttgatcgtg atggatataa tgacttctat atttcatact
     1261 ggtacaatag agtttatttt tattctggta gttcattatg tgttttagat agtattaata
     1321 ttgatggatt tatttcttct acctgcgctg attttgatat tgatgaagat ggaaataatg
     1381 atttgatgtg tggttttact aactttaata atcagtatcg aggttatgta tatattttca
     1441 cgagtgtcat tggtaattat gataccaccc caaaatatgt cctatcagga gaatctgaat
     1501 tggaatattt tgggcagtca ttagattttt taggtgatat aaataatgat ggacttcccg
     1561 aaattgctat tggaaaacga tctggaaatg gtaaagtata tatttattca tttggtgact
     1621 ttcaagatag gattgacgat cgttatgggg tgaagtcaga tgaattcact gtcttaaaag
     1681 catatcctaa tccatttaat aattctaccg taattgaata ttgcttaaag gcgaaatccc
     1741 aggtaaggat tgatatctac gatttatccg ggcaaaaggt cataacccta tcaaatgaag
     1801 tcagacagcc cggtatcaac cgggttgtat ttaatagtca taattatgtt agcggcattt
     1861 acatctgcag aataactatc aacgatattc aggaagaaac gatgaaaata ttattgctta
     1921 aataagactc ataataacaa aacaaggagg tgaatgatga aaacttattt tctcaaaaag
     1981 cctttattag cagtaatagt actttttctt acaggatgta tgacaactgc ttatatttca
     2041 tcattgaccg atactcgata tgcaccagta aagacagatc caatattcat acttatcgca
     2101 gataatgcta gtataaccga tagacaattc tatacttttc taaaaaatga aatgattctc
     2161 aatggattta atattgttga tgaggctcct atagcacgat attttctttt gttccaaact
     2221 gattccaaga cgtctcaaat taactcaaca ttatttttac catccacctc tacgacttct
     2281 ggatatatag gaaatacata ctattctgga tcaacaaaat caacaagtgc ggttccctat
     2341 agttataatt atacagtaaa aaaaatatat ttggatttat acgctgttga ggatgtaaaa
     2401 aatgagaaat acataacggt ttgggaaggc tatattggtg caggtcaaaa ggaatatcag
     2461 acttattctc gagcaattat aaaaagtctt ttagataaat ttggaacaaa ctacgaggct
     2521 cacacaccta tcgatatata ttatgcaaaa taagcataac cattcgctcc agccaacgct
     2581 gggagctgtt cgatgaaagt gaagttttac gagttttctg aagatttgag aataaac
//
LOCUS       DAOOMU010000158         2597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_48856_length_2597_cov_25.382376, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000158 DAOOMU010000000
VERSION     DAOOMU010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48856_length_2597_cov_25.382376"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..1097
                     /locus_tag="PLI55_09725"
     CDS             138..1097
                     /locus_tag="PLI55_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HPB01149.1"
                     /translation="MPKILITGALGQIGSELVPALCQRYGSRNIIASDIRPASDQTIL
                     SAATFETLDVTNFNQIAEIVNKYQITTIYHLAALLSAVAEAHPQKAWHINMNGLYNML
                     EVARQYHCAIFMPSSIAAFGASTPRYMTPQDTIQRPDTIYGVTKVAGELLCDYYFKRF
                     GVDTRGVRYPGIISSKTPPGGGTTDYAVHIFYAAIKQKRYTCFLKPGTYLDMMYIPDA
                     IKAAINLMEADPARLQHRNAFNITAMSIDPEMLAAEIRKHIPDFTMGYAIDEVRQRIA
                     DSWPSHIDDSAARAEWDWRPDYNLETMTTDMLKQLSVKLKNTK"
     gene            1125..1499
                     /locus_tag="PLI55_09730"
     CDS             1125..1499
                     /locus_tag="PLI55_09730"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPB01150.1"
                     /translation="MIYGIGIDLIEVERIARQLIGKTHFREKIFSLNEIAYCEDKSNQ
                     AEHYAARFAAKEALFKALGTGWRGGLNFTEVEVLNDDLGKPAFNFLGKTSQIIEELNL
                     THIHLSLSHLAQIAVAVVILEK"
     gene            1831..>2597
                     /locus_tag="PLI55_09735"
     CDS             1831..>2597
                     /locus_tag="PLI55_09735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPB01151.1"
                     /translation="MSNNKLDIKSLESWLWEAACKIRGPVDAPKYKDYILPLIFLKRL
                     SDVFEDELERLAQNYGSKETVEELLAQDHKLVRFYLPKNARWEVIAKHTTQIGEHLTD
                     AVRLIAKENPKLQGVVDTVDFNATTSGQRILGDDVLKELINVLSKYRLGLNDVEADIL
                     GRAYEYLLRKFAEGSGQSAGEFYTPGEVAILMSHILDPEPGNEIYDPCCGSGGLLIKC
                     FLRFKEKYDSDPALEPLKFYGQEVLPSTFAMAKMNAF"
BASE COUNT          749 a          598 c          555 g          695 t
ORIGIN      
        1 tttttaagaa attatacccg tgactattga tgtgtattta agcgacttaa ttaattgcta
       61 aataaaaagt ggattgaggg atttttagac ggctcgccgc aattcaggcg agccttcttt
      121 ttcaaatgga ggtgccaatg ccaaaaattt taattaccgg cgcgctgggg caaatcggct
      181 cggaactggt tccagctctc tgccagcgat atggctcccg caatattatc gcgtctgaca
      241 tccggccggc aagtgaccaa actattctta gcgccgcgac ctttgaaacg cttgatgtca
      301 cgaattttaa tcaaattgcc gagatcgtta ataaatatca aataactacc atttatcatc
      361 tggcggctct attgtcggct gtagccgaag cccatcctca aaaagcctgg cacatcaata
      421 tgaacggact ttacaatatg ttggaagttg cccgacaata tcattgcgcc atttttatgc
      481 ccagtagcat cgccgcattt ggcgcatcaa caccgcgtta tatgactcct caagatacta
      541 ttcaacgtcc cgacacaatc tacggagtta ctaaagttgc cggggaatta ctttgcgatt
      601 actactttaa acgttttggc gtggacacgc gtggcgtccg ttatcccggg attatctctt
      661 ctaaaactcc acccggaggg ggaacgaccg actacgctgt gcatattttt tatgcggcaa
      721 ttaaacagaa acgctatacc tgtttcttga aaccaggaac ttatctggat atgatgtata
      781 tcccggacgc tatcaaagcc gctattaatt taatggaagc cgacccagcc agattgcaac
      841 atcgcaatgc tttcaacatt actgctatga gcattgatcc ggaaatgctc gccgccgaaa
      901 tccgcaaaca tattccggat tttacgatgg gctatgccat tgatgaggtt cgacagagaa
      961 tcgcagactc ctggccaagc catattgatg actctgccgc ccgcgccgag tgggattggc
     1021 gtccggacta taacctcgag acgatgacca ctgatatgtt aaagcagctt tcggttaaat
     1081 taaagaatac taaataattc aatttttcat tttacattcc aattatgatt tatggaatcg
     1141 gaatagacct gatcgaagtc gaacgcatcg cccggcaatt aattggcaaa actcatttcc
     1201 gcgagaaaat cttctcccta aatgagatcg cttattgtga ggacaaatcc aaccaggctg
     1261 aacactacgc cgcccgtttt gccgccaagg aagctctttt caaagctctc ggtaccggct
     1321 ggcgcggcgg gctgaatttc actgaagtag aagtacttaa cgacgatctc ggcaaaccag
     1381 cattcaactt tctcggaaaa accagccaaa tcattgaaga attaaacctg acccacatcc
     1441 atctttcgct gagccatctg gcacaaatcg ccgtagcggt cgtcattctc gaaaagtagc
     1501 gtcggcttat ttgaagtttc cttcttcggt aaccgtatta atttttgaga aaataattaa
     1561 tgttcaggtc attacgccta gcagaaaaaa ttcatcgaga tggcgattct taataggtaa
     1621 aaatttaaat aatgggccaa caatctataa aaagttgctt aactggtttt atttcagtta
     1681 gccgattaaa ttttgtcatt aaaaaagtga aaccgttaat cgaaattatg tctgctatga
     1741 actctcatcg agcaactccg ttctatttgc taaaaataga aaacaaaatg aaagccttag
     1801 aagatttcta tttaatataa ggactttact atgtcaaaca acaaactcga tatcaaatct
     1861 ctggaaagct ggctctggga agccgcgtgt aagattcgcg gaccagttga cgcaccaaag
     1921 tataaagatt acattttacc gctcatcttc ttgaagagac tctcggatgt gtttgaggat
     1981 gaactcgagc gtcttgcgca aaattatggc tcgaaagaaa cagtcgaaga gttgctcgct
     2041 caggatcaca aactcgtgcg tttttatttg cccaaaaacg ctcgatggga agtgattgca
     2101 aaacatacaa cgcaaattgg cgaacatcta acggatgccg tgcgactcat tgcaaaagaa
     2161 aatccgaaac tgcagggcgt ggtggatacg gtggatttca atgctacaac ttccgggcag
     2221 cgaattcttg gcgatgatgt attgaaggaa cttatcaacg tgctgagcaa ataccgactc
     2281 ggcttgaacg atgtcgaagc cgacattctg gggcgagcgt acgaatacct gctgcggaaa
     2341 ttcgccgaag gttccggaca gagtgcaggc gaattctaca ctcccggcga ggttgccatc
     2401 ctgatgtcgc acattctcga tcctgaacct ggtaatgaaa tctacgatcc ctgctgtggc
     2461 tcgggaggac tcctcatcaa atgctttctg cgcttcaagg aaaaatacga ttctgatcct
     2521 gctcttgaac cgctcaagtt ctatggacag gaagtactgc cttccacatt tgccatggca
     2581 aagatgaatg cctttat
//
LOCUS       DAOOMU010000159         2584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_49210_length_2584_cov_22.359431, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000159 DAOOMU010000000
VERSION     DAOOMU010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49210_length_2584_cov_22.359431"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..>2584)
                     /gene="dnaE"
                     /locus_tag="PLI55_09740"
     CDS             complement(98..>2584)
                     /gene="dnaE"
                     /locus_tag="PLI55_09740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPB01152.1"
                     /translation="IDKNSTLAQLAPSDYKSERHFFRTEKEMIDLFPNSLNAINNSQF
                     LAERCKTDWNYTNTIFPGLSLKETNLANRQLRQLVYDGAKIRYGKIDQVVKKRIEYEL
                     SLITQKGYAPYFLIVYDIVQQTPTTIGRGSGAASIISYCLFITQVDPIKYNLCFERFI
                     HPERVDMPDIDIDFAWDERDAILDYIFKKYGLERTAMVSNQVMLQPRSAIREVAKVYG
                     LSNEEIKAVTKRVGYFARKTELLEWLSTDPRFRNLDLDDTLREIILQSEKIVGVFQVS
                     SVHPGGVIIVPDEIRKYVPVLTATKGVQIVEWEKDQVEDSGLLKIDILGNRSLAVVRD
                     TLKQIGLYRNKFMDYHKIQPVGDPATQDLMQSGRTMGIFYIESPATRLLLKKAGRVDF
                     EHVVIYSSIIRPAANRFINIMLARIHGQPWQLLHPDLKCLEESYGIMVYEEQVSMVAR
                     EIAGFSYAESDYIRKVISRPALAHLVPIWKKKFINGALARGYSPKLAETLWEMIESFS
                     GYSFCKPHSASYAMLSFTCAYLKAHFPAEFLAAVISNQGGYYSTYAYLSEARRWGIQI
                     LPPHINRSWQEYRGRRDKIRMGFMAIRNLQDKAIEAILKERKNGDFKNLDDFLQRVDL
                     DLADAMALTNAGCFAEFEPQLSHREIAFKVASYYLQKGDRRPLPPPVNCQPISRLEKL
                     TLEIESFGFPIAEHPLERYLPVFAGKVKKARDLAQYVGQTVNLLGVYITRKVATTKQH
                     DPMEFVTFEDETDIFECVMFPESYKEFGDLLNWETLFVIRGKVEEAYQTYTVTIEKLA
                     SLQRTVEKMSSGRRMLVKTSSAETHFAKEY"
BASE COUNT          662 a          574 c          664 g          684 t
ORIGIN      
        1 gaaaaacggc gagctacaaa caaatggaag gaagaaatac cgaaagtatt atattgacaa
       61 agacaaaggg taaaattaat aagcgtgatc tgtattctta atattcttta gcaaaatgtg
      121 tttctgcgga actcgttttg acaagcattc gccgtcctga cgacattttc tcgacggtgc
      181 gttgcaggct ggcgagtttc tcgatcgtga cagtgtaggt ctgataagct tcctcgacct
      241 tgccgcggat gacgaaaagc gtttcccagt tcagtaggtc gccgaattcc ttgtatgatt
      301 ccggaaacat aacgcactcg aaaatatcgg tttcatcctc gaaagtgaca aattccatcg
      361 gatcgtgctg tttagttgta gcgactttac gcgtaatgta aacgcccagc aaattgactg
      421 tctgtccgac gtactgcgcc aggtcgcggg cttttttgac tttgcctgca aaaaccggca
      481 gatagcgttc cagcggatgt tccgcaattg gaaatccgaa cgattcgatt tcgagggtaa
      541 gtttttccaa ccgagagata ggttgacagt taactggagg cggaagcggt cggcggtcgc
      601 ctttttgtaa gtaatagctg gcgaccttga aggcaatttc gcggtgggac aattgcggtt
      661 cgaattcggc aaaacaaccg gcgttggtca gtgccatagc gtcggccagg tcaaggtcaa
      721 ctctctgtaa aaaatcatcg aggtttttga aatcgccgtt tttacgttcc tttagaattg
      781 cttcgatggc tttgtcctgt aggttgcgga tcgccataaa tcccatccgg attttgtcgc
      841 gacgcccgcg atattcttgc caactacggt tgatatgcgg tggcaggatt tgaatgcccc
      901 aacggcgggc ttcactcaga taggcgtaag ttgaataata gccgccttgg ttggaaatta
      961 ctgccgccag gaattccgcc gggaaatggg ctttcagata agcacaagtg aacgagagca
     1021 tagcatacga ggctgagtgc ggcttgcaga acgaataacc tgagaaggat tcgatcattt
     1081 cccagagcgt ttccgctaat tttggggaat aaccgcgtgc cagtgcgccg ttgatgaatt
     1141 tctttttcca gataggaact agatgcgcca gtgccgggcg cgaaattact ttgcgaatgt
     1201 aatctgattc agcatagcta aagccggcga tttcgcgcgc taccattgaa acctgttcct
     1261 cgtaaaccat aatgccgtag ctttcctcta aacatttcaa gtcgggatgt aggagttgcc
     1321 agggttggcc atgaattcgc gccagcatga tattaatgaa gcgattagcg gcgggacgaa
     1381 tgattgagga gtaaatcacg acgtgctcaa aatcaactcg tccagccttt tttaataaca
     1441 agcgcgtggc gggactctcg atataaaaaa tgcccatcgt gcggccgctt tgcatcaaat
     1501 cctgcgtagc cggatcgcca accggttgaa ttttatgata gtccataaat ttattgcggt
     1561 acagaccaat ctgtttcaga gtatcgcgca cgaccgccag cgagcgattg ccgagaatgt
     1621 caatcttcag cagaccggaa tcctcaactt gatctttttc ccattcgacg atttggacgc
     1681 ctttggtagc cgttaacacc ggcacgtatt tgcggatttc gtccggtacg atgatcacac
     1741 caccggggtg taccgatgaa acctggaaaa cgccaacgat tttctcactt tgcaggataa
     1801 tttcacgcaa agtgtcatcg aggtcgagat tgcggaaacg cggatcggtg ctgagccatt
     1861 ccaatagttc ggtttttctg gcaaagtagc cgacgcgttt ggtaacggct ttgatttcct
     1921 cgttagacag accatagact ttggcgactt ctcgaatagc cgaacgtggc tggagcataa
     1981 cctggttgga aaccatagca gtgcgttcga gcccgtattt tttgaaaatg taatctagaa
     2041 tagcgtcgcg ctcgtcccag gcgaaatcaa tatcaatatc cggcatatca acccgttcgg
     2101 gatgaataaa gcgttcgaag cacaaattgt atttgatagg atcgacctgc gtaatgaaaa
     2161 ggcaataact gataatggaa gccgctccgc taccgcgccc gatagtggtt ggagtctgct
     2221 ggacgatgtc atagacaatg agaaaatacg gcgcgtagcc tttctgggtt atcagagaga
     2281 gttcatactc aatgcgcttt ttgacaacct gatcaatttt gccgtagcgg attttagccc
     2341 catcgtaaac caattggcgt agttggcgat tggcgagatt ggtttcctta agtgaaaggc
     2401 cggggaaaat cgtattagtg taattccagt cggttttaca gcgttcggca agaaactgcg
     2461 agttgttgat agcattcagg ctattgggga agaggtcaat catttccttc tcggtgcgaa
     2521 agaaatgccg ttcgcttttg taatcgctgg gggcgagttg tgccagcgtg gaatttttat
     2581 caat
//
LOCUS       DAOOMU010000160         2570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_49597_length_2570_cov_7.215905, whole genome shotgun sequence.
ACCESSION   DAOOMU010000160 DAOOMU010000000
VERSION     DAOOMU010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49597_length_2570_cov_7.215905"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLI55_09745"
     CDS             <1..181
                     /locus_tag="PLI55_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01153.1"
                     /translation="NYESAITALKIPFFPLGLCFPAGDVLDAVMVNWMVSFSAPLLLF
                     SQSLFSASLLFTNDQ"
     gene            complement(489..>2570)
                     /locus_tag="PLI55_09750"
     CDS             complement(489..>2570)
                     /locus_tag="PLI55_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HPB01154.1"
                     /translation="SNYNVILDSLNSEHEKKLAELFAPRGEFETTQQYQQRLKNAEQQ
                     KAMLTNLYQQKRQYYEQQIQSEIAQSRKNVTLSHSGTLGRYNIDKQVFPFTYQNQTYE
                     LKVPLNQASDFKDRFETFIIESERQLNAQGDWEYFNYALVDPQRGARYPFGEQRGVTT
                     ATIAQKPTFPPRLQANLSFTEPSGEGFLDAGEKGTVTVEISNSGQGDAFNIIAELKSM
                     SASSGIYFEIMKSIAQIKPNEKKSLIFTLEASDNLPTGTLNFEVKFSEANGFEPEPIH
                     FSLETHALRPPEIVIADFAVRDQSGNGKIERAEPATLTLRLHNRGTGTSHASKAVVQF
                     GANVFPFADSPTEFSLGNIPPGGIKDVEFTFYTNNRVQDKIPINLRIDDARPQFQKTL
                     ALDLQVNVPQKSLEQLTFKGTEKTSVATETPSLSVDIEQNIPQVQISKPDAIAVVIGN
                     RDYTRVKNVEFAIRDAEYVKDYLVKMLGYKEGNILFIKNASKADFEACFGTKDNPRGR
                     LADLVKSGKSEVFIYYSGHGAPGLKTHEGYFVPVDCDPQRVELTGYPLDVFYNNLAQI
                     PAQNFTVVIDACFSGTNIYENISPIMIEILAPEAVLPNGIVFTSAGPQQVSAWYNEKF
                     HSLFTYFFLKGLHNYNADLNKDRQITYQELYQYLNDQNEGVPYWARRLHGVEQTPVFF
                     GTRQDEVLIKY"
BASE COUNT          680 a          600 c          533 g          757 t
ORIGIN      
        1 taattacgaa agtgctatta cagcgctgaa aattccattt tttccgctgg gactttgctt
       61 cccagccgga gatgttcttg atgccgtaat ggtaaactgg atggttagtt ttagcgctcc
      121 tctgctcctc ttttctcaat ctcttttctc agcctcttta ctatttacca atgaccaatg
      181 actaatgacc aatgaccagt gactaatgac caatgcccat tcttaacctt atttttcttg
      241 ttttggtctt aagagaataa accgccaagt tcgccaagaa ccgctaaatg agtaaaatcc
      301 caaactccaa attccaaatc acaaatagcc aaaccaattt ccgcgaaact gtctcctgcc
      361 ttccttctct atctcttttc tcattctctt tactcagtct ctttaccaat taccaattac
      421 caatgaccaa ttaccaatga ctaatgacca atgactaatg accaataacc aatgacagcc
      481 tgttttctct aatacttgat cagcacttca tcctgccggg taccaaaaaa caccggcgtc
      541 tgctctactc cgtgcaggcg tcgcgcccaa taagggacgc cttcattctg gtcgttgaga
      601 tattgatata attcctgata ggtaatttgc cggtctttat tcaaatcggc attataatta
      661 tgtaagcctt ttagaaaaaa gtaagtgaac aggctatgga atttttcatt ataccaggcg
      721 gaaacctgtt gcggtccagc actggtaaat acaattccat tcggtaagac cgcttccggc
      781 gctaagattt caatcattat cggactgata ttctcgtaga tattcgtacc ggaaaagcaa
      841 gcgtcaatga caacggtaaa attctgggca ggaatttgcg ccagattatt ataaaaaaca
      901 tccagcggat agccagtcag ttctacccgc tgagggtcac aatccactgg tacaaaatat
      961 ccttcgtgcg tttttaagcc cggcgcaccg tgcccggaat agtaaatgaa aacctcgctt
     1021 ttgccgctct ttaccaaatc tgccagtctc cctcgcggat tatctttagt accaaagcaa
     1081 gcctcaaaat cagccttgct cgcatttttg ataaacaaga tattgccttc tttatagccg
     1141 agcattttga ccaaataatc cttgacatat tcagcatcac ggatagcaaa ctcgacattc
     1201 ttcactcgcg tataatcacg attgccaatc accacggcaa tggcatccgg cttagatatt
     1261 tgcacttgcg gaatattctg ctcaatgtca accgaaaggc tcggcgtttc tgtagcaacg
     1321 gaagtctttt ccgtgccttt gaaggtcaat tgctctaaac ttttctgagg aacgttgacc
     1381 tgcagatcaa gtgccaacgt tttctgaaat tgaggtcggg cgtcatcaat cctcaaatta
     1441 atgggaatct tatcctgaac gcggttattg gtatagaaag taaattcaac atctttgata
     1501 ccgcctggtg gaatattacc gagtgaaaat tcggttggtg aatcagcgaa ggggaagaca
     1561 ttagcgccaa attgcacgac ggctttgctg gcatgactgg tgccggtgcc acgattgtgc
     1621 agacgtagcg tcagagtagc aggttcagcg cgttcgattt tgccgttacc ggattggtcg
     1681 cgcacggcga aatcagcgat gacaatctcc ggcggtcgca aggcatgagt ttcgagtgaa
     1741 aaatgaatcg gctctggctc aaagccattg gcttcgctga atttcacctc aaaattgaga
     1801 gtgccggtag ggagattatc actggcttca agggtgaaaa tcaggctttt cttttcattc
     1861 ggcttgattt gggcgatact tttcataatt tcaaaataaa taccgctact cgcagacata
     1921 ctcttcaatt cggcaatgat attgaaagca tcaccctgac cgctattgct gatttcgacc
     1981 gtaaccgtgc ccttttcgcc ggcatctaaa aaaccctcgc cggaaggttc agtgaaactc
     2041 agattggctt gcaggcgtgg cgggaaagtc ggcttctggg cgatagtggc agtggtaacg
     2101 cctctctgct caccgaacgg atagcgcgct cccctttggg gatcaacgag agcataattg
     2161 aaatactccc aatctccttg cgcattcagt tgtctttcgc tttcaattat aaaagtttca
     2221 aaccgatctt tgaagtcaga cgcctggttt aatggcactt ttagttcata ggtttgattt
     2281 tgatatgtaa aagggaaaac ctgcttatca atattatagc gtcccaatgt tccggagtgt
     2341 gataaggtaa cattcttgcg agattgagcg atttctgatt ggatttgctg ttcatagtac
     2401 tgcctcttct gctgataaag gtttgtcagc atggcttttt gctgttcagc atttttcagg
     2461 cgttgctggt actgctgggt ggtttcgaat tcaccccgag gggcaaataa ctctgccaat
     2521 tttttttcgt gctcactatt tagagagtca agaataacat tgtaattaga
//
LOCUS       DAOOMU010000161         2556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_49991_length_2556_cov_16.386246, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000161 DAOOMU010000000
VERSION     DAOOMU010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49991_length_2556_cov_16.386246"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /gene="nuoE"
                     /locus_tag="PLI55_09755"
     CDS             complement(<1..1642)
                     /gene="nuoE"
                     /locus_tag="PLI55_09755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPB01155.1"
                     /translation="MQVLDRVKINQEISDLKKTYGDDRSALLPILQELQEKYGYLPDL
                     VMQETAHALGIHPVEVESVASFYSFFRRKEKQGKYVLRLCQTISCDLSGKAKVARQLE
                     NELGIKFGETTRDGMFTLEYTNCLGMCDQGPALLINDRLFARVTPEKVPQIIADCRRD
                     FIKSEFPKVVPSDVRKSGPILSHDLKTGEGLKAALKNTRSGVIGLIRESGLKGRGGAG
                     FPTAVKWQLAGAAQSDKKFVVCNADEGEPGTFKDRYLLFQHTEMIFDGMTIAAYAIGA
                     MKGFVYLRGEYLYLKKYLQEVLEKRRQNNLLGENILGKEGFNFDIEIRMGSGAYVCGE
                     ETALIESLEGYRGEPRNRPPFPVDTGFQNHPTIVNNVETFLDAALICVKGAKWFNEHG
                     TEKSSGTKLFSVSGDCEKPGIYELPFGITIEQLLKEVKGENAKAVQIGGASGRCVARK
                     DFNRTIAFEDISSGGSIIVFGPQVDMLEVAINFMEFFVEESCGQCTPCREGNVKLLEG
                     LKLLEEGRCSASYLNELMRLAETIQLASKCGLGQSSPNA"
     gene            complement(1657..2022)
                     /locus_tag="PLI55_09760"
     CDS             complement(1657..2022)
                     /locus_tag="PLI55_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB01156.1"
                     /translation="MAKVLIVDDDPDFVSAVTIVLESAGHIVSKAYSRQEGMQVFAKE
                     KPDIVVLDVMMQEPDDGFVMAQDLKAKARVPILMLTNVGQVTGLDFDKDSEMVPVDAF
                     ESKPIKPSVLLERINQLLQ"
     gene            2077..2244
                     /locus_tag="PLI55_09765"
     CDS             2077..2244
                     /locus_tag="PLI55_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01157.1"
                     /translation="MDKLMDELGCNTNLPQDWSGSAVDSYGFYDSRYLIILAILYRFS
                     NFLSCQKSAQS"
BASE COUNT          695 a          561 c          532 g          768 t
ORIGIN      
        1 aggcattcgg actggattga cccaaaccgc atttggaagc tagttgaatt gtttcagcta
       61 gccgcatcag ttcatttaaa taggaagctg aacagcgtcc ctcctcaagc agttttaaac
      121 cctcgagcag tttgacattg ccttcacggc agggggtaca ttggccacag gattcttcta
      181 cgaagaattc cataaaattg atggcgacct cgagcatatc aacttgcggt ccgaaaacaa
      241 taatagagcc gccggacgaa atatcctcga aggcaatagt ccggttgaaa tccttacgag
      301 ctacacaacg accggaagcg ccgccaattt gcaccgcctt tgcattttcg cctttgactt
      361 cttttaacaa ttgttcgatg gtaataccaa atggtaattc gtaaatgccg ggtttttcgc
      421 agtcgccgga aacactgaaa agtttggtgc cgctggattt ttcagtgccg tgttcattga
      481 accatttagc gcctttgaca caaatgagag ccgcatccag aaaagtctcg acgttattga
      541 caatagttgg atgattttga aacccggtat caaccggaaa aggcgggcgg tttcgcggtt
      601 caccgcggta gccttccagc gattcgatca gggcggtttc ttcgccacag acataagcgc
      661 cggaacccat tctaatctcg atgtcgaaat tgaaaccttc cttgccgagg atgttttcac
      721 cgagtaggtt attctggcgg cgtttttcga gtacttcctg gagatatttc tttaaataga
      781 ggtattcacc tcgtaaataa acaaaacctt tcatagcgcc aatggcataa gcggcgatcg
      841 tcataccatc gaaaatcatt tcggtgtgct gaaaaagtaa atagcgatct ttgaaggttc
      901 ccggttcacc ttcatcggca ttacagacta caaatttttt atcgctttgc gccgctcccg
      961 ccagttgcca cttgaccgcc gttgggaaac cggcgccgcc gcgacctttc agtccggatt
     1021 cgcgaatcag accgattaca ccggagcgag tatttttcag agcggctttc aggccttctc
     1081 ctgtttttaa gtcatgagat aggattgggc cgctttttct gacatcggat ggtactactt
     1141 tcggaaattc ggattttata aaatcacggc gacaatcggc aatgatttga ggcacttttt
     1201 caggtgttac tctggcaaat aaacgatcgt tgattagcaa agccggtccc tgatcacaca
     1261 ttccgaggca gttagtatat tcaagagtaa acatcccatc tctggtagtt tcaccgaatt
     1321 tgataccgag ttcattctcc agttggcgcg caactttggc ttttccgctt aaatcgcagg
     1381 aaatcgtctg gcagaggcgc agaacgtatt ttccctgttt ttctttgcgt cggaaaaaac
     1441 tataaaagct agcgacgctt tcaacttcga cgggatgaat gcccaaagca tgcgccgttt
     1501 cttgcattac taaatccggc aaataaccgt atttttcctg taattcctgt agaatcggta
     1561 gcagggcaga gcggtcatcg ccataggttt ttttcaggtc ggaaatctcc tgattgattt
     1621 taacgcgatc caatacttgc attggttttt ccttttttat tgcaataatt gattaattct
     1681 ttctaacaga acagatggtt ttatcggttt tgattcaaat gcatctaccg gcaccatttc
     1741 ggaatcttta tcgaagtcca gaccggtaac ctgtccaacg ttggtcagca tcaaaatcgg
     1801 aacacgggct ttggctttca aatcctgcgc cattacaaaa ccgtcatccg gttcctgcat
     1861 cataacatcc agaacgacta tatccggctt ttctttagcg aatacttgca ttccttcttg
     1921 gcgggaatag gctttagata ctatatgccc ggcactttcc aggacaatcg tgacggctga
     1981 tacgaaatcc ggatcgtcat caacgattaa aacttttgcc ataaataatt cctccgttta
     2041 tataataaat caagttgatc agataatatc cgcaaaatgg acaaactaat ggatgaatta
     2101 ggttgtaata caaatctccc tcaggattgg agcggttcgg ctgtagattc atacggtttt
     2161 tacgactctc gatatttgat aatattagct attctgtatc gtttttctaa ttttctatct
     2221 tgccaaaaaa gcgcccaaag ttaggtaaaa ttatttaatt tttcacgtga aattttgagg
     2281 atttattata aattcaatat agacaaagag aaaaagcggt aaagttcaaa gaggtgcttt
     2341 aatattatta aaatcatatt ttgttattgt tttctattta actgaaaatc tttacattac
     2401 tttccgtgaa tgaattaatt gctataatag atgatgagcc agacattctg cagttggtag
     2461 ttttgcaact caagaaaaat ggtttccgaa ctgcgggatt tgccaatgcc agtggatttt
     2521 atagctttct aaatgaaaaa tcgcctgact tggtca
//
LOCUS       DAOOMU010000162         2536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_50562_length_2536_cov_4.950423, whole genome shotgun sequence.
ACCESSION   DAOOMU010000162 DAOOMU010000000
VERSION     DAOOMU010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50562_length_2536_cov_4.950423"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..968
                     /locus_tag="PLI55_09770"
     CDS             75..968
                     /locus_tag="PLI55_09770"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPB01158.1"
                     /translation="MIRTKYSIFDYLIISENDKNWGLYVTGSGNADIPPHTPYPPTKH
                     PKGYMFDWQNGRILTEFQILYITRGSGIFESKATGRKKIEEGYIIHLFPGVWHRYMPD
                     PKTGWKEHWISFNGSIPNDLLNNGILSREKAVLEIGLNEKLINLYQEILELIESQKIG
                     YQEIIASLTFQIVANIQAIEKSKKFFGIEFETTINKAKIFMADRIDKNINMEKLASEL
                     GVGYSWFRRMFRHYTGLPPVQYFLELKLNKAKELLVNTSLPIKEIAVITGFESQFYFS
                     KFFKNRVGMSPIKYREFSRGK"
     gene            1270..>2536
                     /locus_tag="PLI55_09775"
     CDS             1270..>2536
                     /locus_tag="PLI55_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPB01159.1"
                     /translation="MPADTINPPDYLNSNLPVSVRVDDLIARMTLEEKVSQMVNNASA
                     IPRLQIPEYNWWNECLHGVALSGIATVFPQAIALAATWNTDLMFEIADVISTEARAKY
                     HDYISKDERDIHKGLTFWSPNINIFRDPRWGRGQETYGEDPYLTARFGVEFVRGLQGN
                     DPKYFKVISTPKHYAVHSGPEPDRHTFNAIVENRDLYDTYLPAFEACVKEAGAFSVMC
                     AYNRLYGEACCGSPLLLSKILREDWGFKGYVVSDCGAILDIYQNHKIVATAPEAAALA
                     VKAGTDLNCGQVYSSALIEAVQNGLITEHEIDTAVARLFTARFKLGQFDPPELVPYSD
                     IPIEENDSEEHRQLSIRAAQESIVLLKNSKRILPLKKDLKRIAVIGPTADSYQMLLGN
                     YNGTPSRYVTPLQGIRNKVSDQTSVFYEPG"
BASE COUNT          781 a          524 c          492 g          739 t
ORIGIN      
        1 ttacctttaa taatacatag attttatgag tttttgtttg tactttttgg cacttgaaaa
       61 ttgaggaatt ttaaatgatc agaaccaaat actctatttt cgattatctc ataatctccg
      121 aaaatgataa aaattgggga ttatatgtta ctggcagtgg caatgccgac attccacctc
      181 atactcccta tcccccgact aaacatccca aaggatatat gttcgactgg caaaacggac
      241 gaatcctgac tgaattccag attctataca ttacccgggg aagcggtatt tttgaatcaa
      301 aagccaccgg ccgcaagaaa atcgaggaag gctatattat tcacttattt cccggcgtct
      361 ggcatcgtta tatgcccgac ccaaaaaccg gctggaaaga acattggatc tcattcaacg
      421 gttcaattcc taatgatttg cttaacaacg gtattctctc acgagaaaaa gccgttttag
      481 aaattggact caatgaaaaa ttaattaacc tctaccagga aatcctcgaa ctaatcgaaa
      541 gtcaaaaaat cggataccag gaaatcatcg cctccctcac tttccagatc gtagccaata
      601 ttcaggcaat cgaaaaatca aaaaaattct tcggcattga attcgaaact accattaaca
      661 aagcaaaaat ctttatggct gatcgaattg ataaaaacat taatatggaa aaattggcat
      721 cagaactcgg agtaggttat tcctggttta gacgtatgtt tcggcactat accggtttac
      781 caccggtaca atattttctc gaattgaaat tgaataaagc taaggaatta ttggttaata
      841 cttctttgcc aatcaaggaa attgccgtga ttaccggctt cgagtctcag ttctactttt
      901 ctaaattctt taaaaatagg gttggtatgt cacctataaa atatcgcgaa ttttcaagag
      961 gaaaataacc tgtgccgaat atgtgtttcg atcactttaa ttatcacagt cattttttaa
     1021 ttttcccata caaactaaat tactcttaaa gtaatgaaat tttaaatcaa aaatagtgaa
     1081 aatatttaaa gcatatacca tgcgaatcgt ttctgaaaaa ttaatctcaa aatcaatggc
     1141 aagtgcaata ttctaaatta ttaaagtcta ataaattgaa tttaaatgta aaattagtaa
     1201 caggagtaaa cttgtatgcg taggtttatg gcagttctac ttctcggtag taacatttta
     1261 ttcgcccaaa tgccggctga cacaatcaat ccacccgact atttaaattc taatctgccg
     1321 gtatcagtca gagtcgatga cctaatcgct cgtatgactt tagaggaaaa agtttcgcag
     1381 atggtcaata acgccagtgc cattccgcga cttcaaattc ccgaatataa ttggtggaat
     1441 gaatgtctgc acggagttgc cctatcggga attgcgactg tttttcctca ggcaatcgct
     1501 ctcgcagcca cctggaacac tgatttaatg ttcgagatag ccgacgttat ttcaaccgaa
     1561 gctcgcgcaa agtatcacga ttatataagc aaagacgaac gagatattca taagggactg
     1621 actttttgga gccccaacat caacattttc cgtgatccac gctggggacg tggtcaggaa
     1681 acttacggtg aagatcctta tttgaccgct cgttttgggg ttgaattcgt cagaggttta
     1741 cagggaaatg atccaaagta tttcaaagta atttccactc ctaaacatta tgcagttcac
     1801 agcgggccag aacccgaccg gcatactttc aacgccattg ttgagaatcg tgatttatat
     1861 gatacttatc taccggcttt tgaagcttgt gtgaaggaag caggagcttt ttctgtgatg
     1921 tgcgcttata atcgtctcta tggcgaagct tgttgtggtt cacctctttt attatcaaag
     1981 atattacgcg aggattgggg atttaaaggc tatgttgtct cggattgtgg ggcaattttg
     2041 gatatttacc agaatcacaa gatagttgct actgcccccg aggcggccgc gctggccgtc
     2101 aaagccggaa ccgatttgaa ttgtggtcag gtttattcct cagcccttat cgaagctgtt
     2161 cagaatggct taataacgga acacgaaata gacacggcag ttgctcggct cttcacggcc
     2221 aggtttaaac ttggtcagtt cgacccaccc gaattggtgc cctactctga tattcccatc
     2281 gaagaaaatg attccgaaga acaccgacag ttgtctatcc gggcggcaca ggaatcaatc
     2341 gttttattaa aaaatagcaa gcgaattctc cccttgaaga aagaccttaa aagaattgct
     2401 gtaatcggac cgacagcgga ctcctaccaa atgttactgg gcaattataa tggtactccc
     2461 tcccgatacg taactccctt gcaagggatt agaaataagg tttccgatca aaccagtgtg
     2521 ttttatgagc ccggtt
//
LOCUS       DAOOMU010000163         2527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_50792_length_2527_cov_7.740291, whole genome shotgun sequence.
ACCESSION   DAOOMU010000163 DAOOMU010000000
VERSION     DAOOMU010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50792_length_2527_cov_7.740291"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..1483
                     /locus_tag="PLI55_09780"
     CDS             317..1483
                     /locus_tag="PLI55_09780"
                     /inference="COORDINATES: protein motif:HMM:NF040341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HPB01160.1"
                     /translation="MHKSVIVLILLFFINCEKDINNPIIEPEPGIGKTTILIERGLVD
                     YNSYNFPIVSLTYAKISISDIDTSLYFAMSESSPNDTIVILSHGVFNSELNTTGIPIA
                     DECLFIKAYFRNEDTSGTVLLYENLWNYSFIDSVIFEPYKLIGLSDTIRIGKQRYLSW
                     KENYRIDTLKFNVYYKPNINIVYDRFSLSTMDFLYKNSAYDSVKYIAKFIVGIPSMTS
                     EPNIVHYPYSTIELVYESSILKVISRLNYQVEYKLPTWKLNHVIDSLQICRNNWKSLS
                     IHDYSIYQTIKSWYPWYGDTLKIAVYSDTINDIQKVPDLSTIDRSVWERYKTIDELFE
                     LAGLDSTRYELRVRYNSDYYYPEFLYYKLKPPIYTEGGFEYYTWNLEIRRLLVS"
     gene            1553..2416
                     /locus_tag="PLI55_09785"
     CDS             1553..2416
                     /locus_tag="PLI55_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01161.1"
                     /translation="MKSMRKNWIHESHKSGATHSYLNVKWMRYRKFALIIYSFSLINC
                     SPFEDNLFPLEGRIFFKISEDYKQYNRENNPEIFLFLETEKIYGCCNYGISTDCDIDG
                     RKIEVDILGINQPEVCLTAIGPAIARIHLPLSNKLYSLKISGDDFTNNYAVIVTDSSI
                     SVTGDSTVNTKPEIELFWRYPPNSFVYLYGSTFEDSSLANVFLDTLMQVIDITEFYFP
                     QYGEIPYPKSSQGHYWDMPARYFYYNSEDDFNKIEDILRDFKNSYIQDKQGISITIRN
                     WRNRKFQSWLL"
BASE COUNT          894 a          351 c          438 g          844 t
ORIGIN      
        1 gcatccttcg ttttactgaa atttgatttt caggaacaac attttaagac ctgcgatcta
       61 ttttctccca ccactcggcg agaaaggtaa ggtagagaaa acagttatct tatttcttga
      121 caatcggaga ataatttatt aaaattatta caaatggata gaaacccgca aaatcggtta
      181 ggaaattggt ttgtggtgga attatcagtt ttttcgatta agaaaccacc cactaaggtt
      241 actttatata gaaggcagtt ttatatggaa agaattggtt aaatctatga tatgtaataa
      301 agtgtggttg aatattatgc ataaatcggt tattgttctt attcttttat tttttattaa
      361 ttgtgaaaaa gatataaata atccaattat agagccggaa ccaggaatcg gcaaaacaac
      421 gatattaata gaaagaggac tggttgatta taattcttac aattttccta tagtaagtct
      481 tacctacgca aaaattagta tatcggatat tgatacatcc ctatattttg caatgtcaga
      541 atcttctcct aatgatacaa tcgttattct ttctcacggt gtatttaata gtgaactaaa
      601 tacaacaggg atacctatcg cagatgaatg tttgtttatc aaagcctatt tcagaaatga
      661 ggatacgagt gggactgtct tattatatga aaatctctgg aattattcgt ttattgacag
      721 tgtaatattt gagccatata aacttattgg attgtctgat accattcgga tcggtaaaca
      781 acgttactta agttggaaag aaaattatag aatagatacg ttgaaattta atgtttatta
      841 taagcctaat atcaatatag tatatgatag attctctttg tcaacaatgg attttcttta
      901 taaaaattca gcatacgatt cggtaaaata tatagccaaa ttcattgttg gaataccgtc
      961 aatgacttca gaacccaata ttgtacatta tccatatagt acaatagaac ttgtttatga
     1021 gagtagcatt ttaaaagtaa tatcaagatt aaactatcaa gttgaatata aattaccaac
     1081 ctggaagtta aaccacgtta tagattcgct gcaaatttgc cgtaataatt ggaaatcatt
     1141 atcaattcat gattattcga tatatcagac tattaagtcc tggtatccct ggtacggtga
     1201 caccttaaaa atcgcggtct attcagatac aataaatgat attcagaaag tacctgattt
     1261 atctactatt gatcgttcag tttgggaacg atataaaacg attgatgaac tatttgaatt
     1321 agcgggttta gattcaacta gatatgagct ccgagtaagg tataactcag attattatta
     1381 tccagaattc ttatattata aattgaaacc accaatatat accgagggag gatttgagta
     1441 ttatacctgg aatttagaaa tcagacgact acttgttagt tgaaactgct aaataaaata
     1501 agcacataac cgttatttag agttaatata tgatttaaag tttgatgagt ttatgaaaag
     1561 tatgagaaaa aattggattc atgaatccca taaatccggc gcgactcata gctacctaaa
     1621 tgtaaaatgg atgcgttata gaaaatttgc gcttattatt tattcattct cgctaataaa
     1681 ctgcagtcca tttgaggata atttatttcc tttagaggga agaatatttt tcaaaatatc
     1741 ggaggactat aagcaataca accgtgaaaa taaccctgaa atatttttat ttctggaaac
     1801 tgagaaaata tacggttgct gtaattatgg catttctacg gattgtgata tagacggtag
     1861 aaaaattgag gtggatattt taggaataaa tcaaccggaa gtttgtttga cggcaattgg
     1921 accggcaatc gcgagaattc atctcccatt atctaataag ttatattcat taaagatatc
     1981 aggcgatgat ttcactaata attatgctgt gatagttaca gattcatcaa tatcggtaac
     2041 gggggactct accgtcaata cgaagccgga aatagaatta ttctggcgct atcctcccaa
     2101 ttcatttgta tatctgtatg gtagcacttt tgaagattct tctttggcaa atgtattttt
     2161 ggatactttg atgcaagtta tagatattac tgaattttat ttccctcaat atggagaaat
     2221 accttatcca aaatcatctc aaggtcatta ttgggatatg cccgcaagat atttttatta
     2281 taattctgaa gatgatttta ataagattga agatatttta agagacttta aaaatagtta
     2341 tatacaggat aaacaaggaa tatctataac aatacgaaat tggcgtaata ggaagttcca
     2401 aagttggtta ttataaaaag tatctaacca tcagatgaag gcaacgaggt aagctctgtg
     2461 ttatgattta attggatatg gaaaattgtt aagtttaaaa taagagtcaa aaagaaaaga
     2521 gccaaga
//
LOCUS       DAOOMU010000164         2486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_51966_length_2486_cov_6.786919, whole genome shotgun sequence.
ACCESSION   DAOOMU010000164 DAOOMU010000000
VERSION     DAOOMU010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51966_length_2486_cov_6.786919"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>572
                     /locus_tag="PLI55_09790"
     CDS             <1..>572
                     /locus_tag="PLI55_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01162.1"
                     /translation="IGDPIAAIGWGKMVAEIAVDPLAAGNKVLKFTPNNYNAAPVLEV
                     TLPAGKTLADYSTFKFKGYFAQGDVGWKDIIVEAYQTLPTGQAYKNEAAKIGSWNRAK
                     GGSTAWEDISISISGSSSLTGTIYLAFGINCAGTGDIGGSGLTTIWYADNIQLVLSPL
                     VTQWGKAPGREFWPVGNDSNTVAGEAILGD"
     assembly_gap    573..672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <673..>2486
                     /locus_tag="PLI55_09795"
     CDS             <673..>2486
                     /locus_tag="PLI55_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB01163.1"
                     /translation="VKIDKGEPITVPEAPWQAFAFDKWGKAPGREFWPVGNDSNTVAG
                     EAILGDGTNTSSGGWASIRGSFDGDPVTVPSDKAVIISGSMELIGGGAGSDYTFMRYA
                     LGFTDSLELVDQYTDSARWVSVADSAKNANKTNGYLFTPVSGTGTMSNSWRGENGGAG
                     TVWRVNYGDWVSTNNPNSPQIAAVRQAPRNAEMVAGVYKWAISIQDMGDGTNEVRWYM
                     VEENDAYWFGGTVIDEAQITNKVNGVMFGFNGGEYTRVDFWNVKIDKGEPITVPEAPW
                     QAYKVDQWGFSGGNLGGWDLTPSIVGEVSIGGTAAPTGWVTLRGGFDPFELNDEYALK
                     VSGQIELVGGGFDNAGSLRFGLFYSDSAGTTWQDSTLDSNWVWRGTDNYHSGYLITPP
                     SGSNVATWSNGKGTWGSIANGKWWDISASTAHALGNPVQDPASGPAGAGMYDFTISVS
                     KGTGGDMIVFTISKTDGTYYFAHTTTSPVTSVVEKINSIGFAINNTTATAMNLYEVTV
                     DRGEHFDGVDTKTAGVPKIYALKQNYPNPFNPTTNIRFDLPKDSDVNLVVYDLMGREV
                     AKLVNNRLNAGYYTINFNAANLPSGVYIYRLKAGDFVS"
BASE COUNT          594 a          539 c          667 g          586 t
ORIGIN      
        1 tatcggcgac cccatcgccg ctattggttg gggtaaaatg gtagccgaaa ttgcggttga
       61 tcctcttgcc gctgggaata aagtcctaaa atttacgcca aacaactaca atgcggcgcc
      121 agtactcgaa gtgaccctgc cagccgggaa gacgttagcc gattatagta cctttaaatt
      181 caaggggtac tttgctcagg gtgatgttgg ctggaaagac attattgttg aggcttatca
      241 gactttgccc acagggcagg catacaaaaa tgaagctgct aaaatcggca gctggaatcg
      301 cgccaaaggt ggctcgacag cctgggaaga tatctcgatt agtatcagtg gtagctccag
      361 tctcacgggt acgatatatc tcgcttttgg tatcaactgt gcgggtacag gagatatagg
      421 cggctcagga ttaaccacta tatggtatgc cgataacatc cagcttgtac ttagtccttt
      481 ggttactcag tggggcaagg ctccgggacg tgagttttgg ccggttggca atgattcgaa
      541 tacggttgcc ggtgaagcca tactcggcga tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnacgttaag attgacaagg gcgaaccgat cacggtgccg gaagcgccct
      721 ggcaagcatt tgcctttgat aaatggggca aggctcccgg acgtgagttc tggccggttg
      781 gcaatgattc gaatacggtt gccggtgaag ccatactcgg cgatggtaca aatacttctt
      841 ccggtggctg ggccagcatt cgcggtagtt ttgatggtga tcccgtcacg gttccttcgg
      901 ataaggctgt gatcatttcc ggatccatgg aactcatcgg cggcggagca ggcagtgatt
      961 acacctttat gcgctatgcc cttggtttta cggacagcct tgagctggtc gatcaataca
     1021 ccgattcggc ccgctgggtt tccgtagccg attccgcgaa gaatgcgaac aagacgaacg
     1081 gttacctctt tacgcccgta tcggggactg gaacgatgtc caacagttgg cgcggtgaga
     1141 acggcggagc aggaacggtc tggcgagtca actacggcga ttgggtcagt actaacaatc
     1201 cgaacagccc ccagatagca gctgtaagac aggcgccgcg caatgctgaa atggtcgcgg
     1261 gtgtctataa gtgggcgatc tccatccaag atatgggcga tggcaccaat gaggtccgct
     1321 ggtatatggt ggaagagaac gacgcctact ggttcggtgg caccgtcatt gacgaagccc
     1381 agataaccaa taaggtcaac ggcgtgatgt tcggatttaa cggtggagag tacactcggg
     1441 ttgatttctg gaacgttaag attgacaagg gcgaaccgat cacggtgccg gaagcgccct
     1501 ggcaagccta taaagttgat cagtggggat tcagcggagg taatctgggc ggttgggact
     1561 tgactcccag catagttggc gaagtatcta ttggaggtac tgcggctcct accggatggg
     1621 taacactacg cggcggattt gatcctttcg aactgaacga tgagtatgcc ttgaaagtat
     1681 cgggacaaat cgaactggtc ggcggcggtt tcgacaatgc aggcagtctg cgttttggat
     1741 tgttctacag cgacagtgcg ggaactacct ggcaggattc aaccctggat tccaactggg
     1801 tctggagagg tacggataat tatcattccg gttatctaat cacacctcca agcggttcta
     1861 atgttgcaac ctggtcgaat ggcaaaggaa catggggttc tatcgcaaat ggtaaatggt
     1921 gggatatcag tgcttctaca gctcatgctc tcggcaatcc tgttcaggat cctgccagtg
     1981 gacccgccgg agccggaatg tatgatttta cgatttcagt atccaaaggc acgggcggag
     2041 acatgatagt atttactatt tccaagacgg atggtactta ctactttgcc cataccacca
     2101 ccagtccagt aacatcagtt gtagaaaaga tcaatagtat aggattcgct attaacaata
     2161 cgacagctac ggctatgaac ttatacgaag ttacggttga cagaggcgag cattttgatg
     2221 gtgttgatac taaaactgcc ggcgttccga agatctatgc tttgaagcag aactatccga
     2281 acccattcaa cccgacgacc aatattcggt ttgatttacc gaaagacagc gatgtcaatt
     2341 tggtagtcta tgatttaatg ggtcgtgaag ttgccaagct ggtcaataac cgtctgaatg
     2401 ccggttatta cacgattaac ttcaatgcgg cgaatctgcc ctccggagtt tatatctata
     2461 gactcaaggc tggtgatttt gtcagc
//
LOCUS       DAOOMU010000165         2481 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_52121_length_2481_cov_13.526381, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000165 DAOOMU010000000
VERSION     DAOOMU010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52121_length_2481_cov_13.526381"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLI55_09800"
     CDS             complement(<1..190)
                     /locus_tag="PLI55_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007039818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HPB01164.1"
                     /translation="MESLKITPTELLEYYFCPRFIYFMNVLKIPQYEDRRYKVQKGRE
                     VHAERQSHNKDYLWKKVGA"
     gene            complement(242..547)
                     /gene="cas2"
                     /locus_tag="PLI55_09805"
     CDS             complement(242..547)
                     /gene="cas2"
                     /locus_tag="PLI55_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014780681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPB01165.1"
                     /translation="MKPTDQLVWVIYDISKNKIRTKVIKATELAGLYRVQKSVFLGTI
                     NHTLLDELVMKVKDLIDPDTDSVYIFPMCEPDFKKVITAGQAFDKKLVTDEVKALLF"
     gene            complement(534..1649)
                     /gene="cas1"
                     /locus_tag="PLI55_09810"
     CDS             complement(534..1649)
                     /gene="cas1"
                     /locus_tag="PLI55_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HPB01166.1"
                     /translation="MNRGSAYTFYYYPSTSFVGIPPPHRPSSTPRFQSWTIHPWNPEM
                     QIILSTFGSSISKKGSMFEVFSQGERHTISPNKVSSILIFTGTNITSDAIELALQSNI
                     DILILNKYGDPIGRFWHARMGSTTRIRRAQLQLVKSAAGIQYGLQWISGKFDNQIKLL
                     TTMRERRTRLSAEITNALNTIQKARESVDKLEGSIEEVRQQLLGIEGSAGKAYWDIFA
                     KFVPEHFAFNGRSRQPAKDEFNAMLNYGYGVLYGVVEKAVVVAGLDPYIGFIHTDNYN
                     KVSLVYDVIEKYRIWAEETILDLFVKKAIRKDLFDKLANGYNLGAEGKKVFLPALNEY
                     LDSPVHYKSRNVRRRDIIQLDLHRFAQELLEKYNETD"
     gene            complement(1686..2042)
                     /locus_tag="PLI55_09815"
     CDS             complement(1686..2042)
                     /locus_tag="PLI55_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01167.1"
                     /translation="MTDETQTETTKERKMTKTSFAETINSGKLMATGLQANAEAIARR
                     GISADFIATLNTTIGNVERLNAEQESLKANLKAKTAELDAELANLNKMYSEAKKVVKL
                     AIDKTRWLEFGVTDKH"
     gene            complement(2039..>2481)
                     /locus_tag="PLI55_09820"
     CDS             complement(2039..>2481)
                     /locus_tag="PLI55_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01168.1"
                     /translation="YYYEESDLVSHDFSRGHSASQSPLQHSTISILDNRIFLPRQPFS
                     HSGHSPSYLGQPFPNVGLSPSYLGQPFPNVGQSMSDLGRPFSGIGRKISDYGLRFTGD
                     GNVHSGVVITLPKHLSGASSQSGLKMLSDISKTLYKINSQEVSL"
BASE COUNT          637 a          525 c          595 g          724 t
ORIGIN      
        1 ttgcgccgac ttttttccat aggtaatctt tattatgaga ttgacgttcg gcatgtactt
       61 ctcgtccttt ttgaacttta taccgtcggt cttcgtattg cgggatcttt agtacgttca
      121 taaaataaat gaaccgcggg cagaagtaat attccaataa ttcagtcgga gtaatcttca
      181 gggattccat aaaaagtgcc gctatctcat actacacgaa ttaattcgtg gactagggtg
      241 gttaaaataa taaggctttg acttcatcgg taaccaattt tttatcgaaa gcttgtccgg
      301 cggtgatgac tttcttaaaa tccggttcac acattgggaa gatatagacg ctgtcagtgt
      361 ccggatctat caagtctttg accttcataa ctaattcgtc cagcagagtg tgattgatcg
      421 taccgagaaa tactgacttc tggacgcggt aaaggcctgc taattcagta gcttttataa
      481 ccttcgtccg gattttattc ttggaaatat cataaattac ccatactaat tgatcagtcg
      541 gtttcattgt atttctccag taattcctgc gcaaagcggt gcagatcgag ttgaattata
      601 tctcgccgcc gcacattacg cgatttataa tgaacaggcg agtctaaata ttcattcaga
      661 gccggtagaa aaactttttt accttccgct ccgagattgt agccattggc tagtttatca
      721 aataaatcct tgcgaatagc ttttttgaca aataaatcca gaatagtttc ctcagcccag
      781 atgcgatatt tctcgataac atcatagacc agcgagactt tgttgtaatt atcggtatga
      841 atgaaaccga tatagggatc gaggccagca acgactacgg ctttctcgac tactccgtaa
      901 aggacaccat agccgtaatt cagcatggca ttgaactcat ctttcgccgg ttgacgactg
      961 cgaccattga aggcgaaatg ctccggaaca aatttcgcaa atatgtccca gtaggctttt
     1021 ccggctgaac cttcgatgcc gagtagttgc tgacgcactt cttcgatcga accttccagt
     1081 ttgtcaacgc tttctcttgc tttctgaata gtgttcaggg cgttggtaat ttcggccgag
     1141 aggcgggtgc gacgttcccg catagtagtc agtaacttta tctggttatc gaatttgccg
     1201 gaaatccatt gcagaccata ctgaattcct gcggctgatt ttaccagttg taattgtgcc
     1261 cgacgaatgc gggtagtcga gcccatccga gcatgccaga agcggccgat tgggtcgccg
     1321 tatttattta gaatgagaat gtcaatatta gattgtaaag ccagttcgat ggcatccgaa
     1381 gtaatgttag ttccggtaaa gatcaggatc gaagagactt tattgggcga aatggtatgg
     1441 cgttcgcctt gcgaaaagac ctcgaacatc gagccttttt tggaaatgga tgaaccgaag
     1501 gtggaaagga tgatttgcat ttcaggattc cacggatgga ttgtccacga ttgaaatcgt
     1561 ggggtgctgg aggggcgatg gggaggagga atgcccacga atgaagtcga gggataatag
     1621 tagaaagtat aggcactgcc ccgattcacg gagcagtgcc tggttcaaat ggttacctgc
     1681 gaatctcaat gtttgtccgt aactccgaat tccagccagc gggtcttgtc aattgcgagt
     1741 ttgacgactt tcttagcttc ggaatacatt ttattcaaat ttgccaattc ggcatccaat
     1801 tcggcagttt tggccttcaa attggccttc aaggactctt gctcggcgtt gagccgctcg
     1861 acatttccga tggtcgtgtt caaagtagcg ataaaatcgg cgcttatacc ccgcctggcg
     1921 atcgcctcgg cattagcctg cagaccggta gccatcagtt taccgctgtt aatcgtttct
     1981 gcgaaacttg ttttggtcat tttgcgctcc tttgtggtct cggtttgagt ttcatctgtc
     2041 ataatgacac ctcctgtgaa ttaattttat atagtgtttt gcttatatcc gagagcatct
     2101 tcagaccact ttgtgaagac gctccagata aatgtttggg taaagttatt actactcctg
     2161 aatgaacatt tccatctccg gtgaatcgca gtccgtaatc ggaaattttc cgtccgattc
     2221 cggaaaaagg tcgtcctaag tcggacattg attgaccgac attcgggaaa ggttgtccta
     2281 aataggacgg agagagaccg acattcggga aaggttgtcc taagtaggac ggagagtgtc
     2341 cggaatggga gaaaggctgt cgcggcaaga atattcgatt gtccaagatt gaaatcgtag
     2401 agtgctggag gggcgattgg gaggcggaat gcccacgact gaagtcgtgg gatactaggt
     2461 cggactcctc ataatagtat c
//
LOCUS       DAOOMU010000166         2465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_52642_length_2465_cov_3.392116, whole genome shotgun sequence.
ACCESSION   DAOOMU010000166 DAOOMU010000000
VERSION     DAOOMU010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52642_length_2465_cov_3.392116"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..1361
                     /locus_tag="PLI55_09825"
     CDS             444..1361
                     /locus_tag="PLI55_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003682785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HPB01169.1"
                     /translation="MNKKERLAEKEKFLRLLNNFVGELGGYVSGTDGQWTVKGFIDIY
                     RNIYTISSDTKIVSKILEIHLFPRILQFAQDNGYSIVLTEHQNYYPDLSFIKQENPTI
                     KFAVDLKTTYRDPDFPGHVNGFTLGSHGSYFRERNSKKNIQFPYAEYSGHFCLGAIYT
                     RTSTKDLDETQIFRVKEIEAEEYRGRESRYRITSVDNLHSITSVVRDFQFFVCEKWRL
                     ASDKQGSGNTANIGSITFIEDIMAGNGVFARLGEQWFDEYWMNYNVTTWMKKGKATPI
                     RSIEDFLDFKGGDKSLIVPVKTKKKGNEK"
     gene            1367..2305
                     /locus_tag="PLI55_09830"
     CDS             1367..2305
                     /locus_tag="PLI55_09830"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HPB01170.1"
                     /translation="MQVIVPPIKSQGIKTKLVPWIQLLVPDVKGRWIEPFFGTGVVAF
                     NSGFSQALLNDINPHIIRFYQAIKDRDITPESVREYLERESKLLAEANDKGYAHFRLV
                     KDRFNKQHDPLDFLFLSRAGFNGMMRFNKKGEWNIPFCQKPERFSKSYITKIVNQVNK
                     VACLIQPEWIFTAESFEQTILKARSNDIIYCDPPYIGRYVDYFSGWTEADELRLFSLL
                     SETPAKFILSTWHHNDYRENKFIHSLWSKFNIVTRDHFYHGGGKIENRKSIVEALVFN
                     FEANIEKHNAYVQPKPEQMVLFEQRSEYIKRKLRRG"
BASE COUNT          743 a          486 c          567 g          669 t
ORIGIN      
        1 gcgctatacc gatgaacggg aaatcgtgtt ctggatgaag ggcgagacgg cctttgtcca
       61 gcaacgcgct ccgagtggtg aatggcagat tttgtacaat aactgtcgga taatttcaaa
      121 acggtaatgg aatacgctga tagccctggc gcggacttcc agagagacgg gaggcgtttc
      181 tttcctcgct gcgctcggaa atgcattaac gttgttaatg caatccggtt agatccagat
      241 gatttctacg gcaggtctga gaacctggca gaaccgagat aaaaaacctt ctgaaggtct
      301 atcaataaat tcatggctga tgataatctt cggttcaact ttcagaaggt tggcgctatg
      361 ccaaaatcga acttgacaaa agtgcgatga ttgcttaata tatatctgtt tataaaatcc
      421 ggataaattg gcaagggaag ttaatgaata aaaaagaacg gctggcagag aaagagaaat
      481 ttttaagact gctgaacaat tttgtcggtg aactgggtgg atatgtttcc gggactgacg
      541 gccaatggac ggttaaggga tttatcgaca tataccgcaa tatttacacg attagttccg
      601 atacgaaaat tgtgtcgaag attttagaaa tacacctgtt cccgcgcatc ctgcaatttg
      661 cgcaggataa cggttattca attgtactga cagagcatca gaactattac cccgatctga
      721 gtttcatcaa acaggaaaat ccgactatca agtttgcggt tgaccttaaa acaacttatc
      781 gcgatccgga tttccccggt catgtcaacg gttttacact ggggagtcat ggatcttatt
      841 ttcgggaaag aaattcaaag aaaaacatcc agtttcctta tgctgaatac tccggtcatt
      901 tctgcctggg agcgatctac acccggactt ctacgaaaga tttggatgag actcaaatct
      961 tccgcgttaa ggaaatagag gcagaggaat atcgcggccg ggagagccgc taccggatta
     1021 cttctgtcga taatttgcat tcaattactt cagtcgtcag ggatttccag tttttcgttt
     1081 gtgaaaaatg gcgcttggcg agcgataaac agggatcagg caatacggcc aatatcggtt
     1141 cgataacatt tattgaggat attatggccg gaaatggcgt ttttgccagg ctcggtgaac
     1201 agtggttcga tgagtattgg atgaattaca atgttactac ctggatgaaa aagggaaaag
     1261 ccacaccgat tcgttctatc gaagattttc tggatttcaa gggtggtgat aaaagtctga
     1321 tagtaccggt taagacgaag aaaaaaggga atgaaaagta gatagtatgc aggtaatcgt
     1381 acctcctata aaaagtcaag gaattaaaac caaactcgtt ccatggattc aattgctagt
     1441 tccagacgtc aaaggtcgtt ggatcgaacc gtttttcgga accggtgtcg ttgctttcaa
     1501 ttccggtttc tctcaggctt tgcttaatga tatcaatccg catataatcc ggttttatca
     1561 ggctatcaaa gatcgagata ttactccgga gtcggtgcgc gaatatcttg aacgggaaag
     1621 taaattactg gctgaggcca atgataaagg ctacgcccat tttcgattag taaaggatcg
     1681 tttcaacaaa cagcacgatc cgttggattt cctttttctg tcgcgagcgg gattcaacgg
     1741 gatgatgcgt tttaataaaa aaggagagtg gaacattccc ttttgccaga aaccggaacg
     1801 tttctcaaaa tcctatatca ccaaaatcgt gaatcaggtt aacaaagtgg cctgcttgat
     1861 tcaaccggag tggattttca cggcggaatc attcgagcag acgattctga aagccaggtc
     1921 gaatgatata atttactgcg acccacccta tatcggccgg tacgtggatt attttagtgg
     1981 ctggacggaa gctgacgaac tgcgcctgtt cagcttgctt tcagaaacac cagccaaatt
     2041 tatactctca acctggcatc ataacgatta ccgcgaaaat aaatttatcc attcgctctg
     2101 gtcaaaattc aacatcgtca cacgtgatca cttctatcac ggcggcggca agatcgaaaa
     2161 ccggaaatca atcgtcgaag ccttagtctt taacttcgag gccaatatcg aaaaacataa
     2221 tgcctacgtc cagcccaaac cggaacaaat ggtgttattc gagcagagat cggagtatat
     2281 aaagaggaaa ttgaggcgag gataacttga taatgagtat gggataaaat atgaagacaa
     2341 tagtagcata cttagtctcg attgttattt tacttgtgat agaaccgata atagtatttc
     2401 tcttctttcc aatattagcc tatcttagaa aaataacaat cgagactaag tatgctacta
     2461 ttgtc
//
LOCUS       DAOOMU010000167         2381 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_55328_length_2381_cov_9.612640, whole genome shotgun sequence.
ACCESSION   DAOOMU010000167 DAOOMU010000000
VERSION     DAOOMU010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55328_length_2381_cov_9.612640"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLI55_09835"
     CDS             <1..236
                     /locus_tag="PLI55_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01171.1"
                     /translation="LNRMICQNQFSPTCGGLQIGLSWYSEEPIFGEVKWNLIGLIKKF
                     FTSLRLNSLKIGGCPGLINLIFNDLLINMKMLF"
     gene            294..2141
                     /locus_tag="PLI55_09840"
     CDS             294..2141
                     /locus_tag="PLI55_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01172.1"
                     /translation="MKKIIVGLLFFLVMQEFGLSSNKVSQVAATWLLIPVSPTLNGMG
                     EIGVGLPSADIYAGYFNPANGIYGYRGLSLAYSGYKTNWLPQLSDFKFGHSVLGLSIL
                     PKSLPFQMVFSLYQTILDFGLQTPTDEDGNIIYKYRSLYTADAFSLASRYRNTFIKIP
                     IDISAGITMKKVVQSYAPGDSGKINNVFCDAGVLLSSPLKLGSNSNIKLDIIPAFGYS
                     ISNIGKEIYYKSPDMADPAPRYARVGLSLTMNLVLNNDWNLVSYRWGRSAGDILYHPR
                     NDGNPPFQYQSGLGDIDFVKNVLDYQGNEEVEISWGQEVVFLDFYALRWGRYLDLGGD
                     LKERRTGYSFYSTGLLRLIGYVTQCDLINKVSDHLTVSYNYAKLIGDSYSSLWTPRSD
                     TKFKSWTFTLNNIDWKSRPSEREVQKEIHPKFPISIITGINIADVRINKQEYKEKDIK
                     AIKMNPGYKIGIESKFGLIITGIAFDQRGANYLGEAKIDPDIHLFDFNIIDNYNYVSL
                     YGLLSIPVIKRIRIFSGIETGDCIRREMILQIAAGELSDENIEEHEDLPPTNINWDYG
                     LKVGADFMIFKSLGLRAAYYHGFNYVFKEVTALPNNKHRNIEFSLIYQL"
     gene            complement(2197..>2381)
                     /locus_tag="PLI55_09845"
     CDS             complement(2197..>2381)
                     /locus_tag="PLI55_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01173.1"
                     /translation="IYNLKGELIAKLTDDYFTPGYHSITWNGCDQYGQLSPTGIYFAE
                     IRVQGRTQTRKMLCIK"
BASE COUNT          743 a          380 c          487 g          771 t
ORIGIN      
        1 gattaaatag gatgatatgc caaaaccaat ttagccccac ctgtggcggg ttgcaaattg
       61 gtttgtcatg gtattcagag gaaccaattt ttggtgaagt taagtggaat ctaattggtt
      121 tgatcaaaaa attctttacc tcgttaaggt taaactctct aaaaattggg ggatgtcccg
      181 gattaattaa cttgattttt aatgatctat taattaatat gaaaatgtta ttttagagtt
      241 taatgtaata atgtctattt tccataatca gatgaaataa attgagggaa gtgatgaaga
      301 aaataatagt tggattacta ttttttttag taatgcagga atttggatta tcctccaata
      361 aagtgtcaca ggtagcagca acttggttgt tgatcccggt ttcaccgact ttaaatggta
      421 tgggcgaaat tggagtaggc ttgccctctg ctgatattta tgccggatat tttaatccgg
      481 ctaatggaat ttatggttat cgcggacttt cgcttgctta ctcaggttac aagactaatt
      541 ggttaccgca actatctgac tttaaatttg gtcactctgt tttaggttta agtattttac
      601 cgaaaagttt accttttcag atggtgttta gtttatatca gacaattttg gactttggat
      661 tacaaactcc cacagatgaa gatggtaata taatttataa gtatcgatca ttatataccg
      721 cggacgcttt ttcacttgca tctcgctaca gaaatacttt cattaaaata cctattgaca
      781 tttcagccgg aataacgatg aaaaaagttg ttcaatctta tgcccctggt gattccggta
      841 aaataaataa tgttttctgt gatgctggcg tactattatc atcacctttg aaactaggat
      901 caaatagtaa tataaaactg gatattatac cggctttcgg ttatagtatt tcaaatattg
      961 gtaaagaaat ctattacaaa tctcccgata tggccgatcc ggctcccaga tatgcccgag
     1021 tcgggctttc gcttacaatg aatttggtat taaataacga ttggaattta gtcagttacc
     1081 gatggggaag atcagcgggc gatattttat accatccccg gaatgatggt aatccaccat
     1141 ttcaatatca aagcggatta ggtgatattg atttcgtaaa aaatgtcctt gattatcaag
     1201 gtaacgagga ggtagagatt tcgtggggac aagaggtagt ttttctggat ttttacgcac
     1261 ttcgatgggg aaggtattta gaccttggtg gagaccttaa agaacggaga accggttata
     1321 gtttttacag taccggatta ttgcgtttga ttggatatgt aactcaatgc gatttaatca
     1381 acaaggtttc ggatcatttg acagttagct ataattatgc aaaattaata ggcgacagct
     1441 attccagctt gtggacacca agatcagata caaaattcaa atcctggact tttacgctga
     1501 ataatattga ctggaaatcc agaccgagtg aaagggaagt tcaaaaagaa atccacccga
     1561 aatttccaat ctcgattatt accggtataa atattgccga tgttcgtata aataaacaag
     1621 aatacaaaga aaaagatata aaggcaatta agatgaatcc cggctataag atcgggattg
     1681 aatcaaagtt tggattaatc attaccggaa ttgcatttga ccaacgcggc gctaattact
     1741 taggtgaagc taaaatagat ccagatattc atctttttga ttttaatata attgacaatt
     1801 ataattacgt ctctttatac ggcttgctat ctattcccgt aataaaacga ataagaattt
     1861 tcagtggaat agaaacaggc gattgtatta ggcgggagat gattttgcaa attgcggctg
     1921 gagaattatc cgatgaaaat atagaagagc acgaagattt gcctcccact aatattaatt
     1981 gggattatgg attgaaggtt ggcgcagatt ttatgatttt taaatcattg ggattacgtg
     2041 ccgcttatta tcatggcttt aattacgtat ttaaggaagt tacggcgtta ccaaacaata
     2101 aacaccgcaa tattgagttc agcctgatct atcaattgtg atcgcaaagt gccttttatg
     2161 ctttcaacga actaaattaa catagcaagc ctaagtttat tttatacaca acattttgcg
     2221 ggtttgagtc cgaccttgga ccctgatttc ggcgaagtag atgccggtcg gagataattg
     2281 tccgtattgg tcgcatccgt tccaagtaat tgaatggtaa ccgggtgtga aataatcatc
     2341 ggtcagtttg gcgattagct cgcctttgag attatagatt g
//
LOCUS       DAOOMU010000168         2370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_55692_length_2370_cov_19.457451, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000168 DAOOMU010000000
VERSION     DAOOMU010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55692_length_2370_cov_19.457451"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..>2370
                     /locus_tag="PLI55_09850"
     CDS             131..>2370
                     /locus_tag="PLI55_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01174.1"
                     /translation="MSKKTKKSTSAKNVPPQNKKPVNWLATINPRKATILAFLIVLLI
                     LIIFYKPYVIDRLEPAGGDRMASIAQSKFINDYREQTGEIPLWNPAIFCGVPIYYALW
                     SNSFHLDYLISKISKVIDWKLSWFILAMVGMLLIFWLLEFPWYYALLGTIAFLFYPHY
                     QALITVGHFAKVRAVCAMPLAVFGFLYLVKKRNFLSFLLFLIFFSLQLRTQHYQIVFY
                     TLLVFMALGIRQIVEWVKTKQAQKIYVSLGLFVAGLVTSVLMSAQPLFVVNEYTPYST
                     RGGQAINLKEDATQAEVKSSGVTFEYATRWSLNPKELATLIVPRFYGGTSQEPYTGKA
                     YPQLRGQPIPGYWGDMPFTQSCEYMGILIVILALLGLWYYRKDGVVISLFILLVFSLL
                     LGFGSHFPILYKPLFKYLPYFSKFRVPSMILILVDFILVILSVYGLKGLVEKFDLAKY
                     KSALMVSGFFILFGLFFLLAPGVLSYTSAQDAQYMNNPQVIEMLRTARREFLQADTLR
                     MLVFVICFVALLVLYKMKKIRPDFLIGAIVILVAVDMINVSKRFLRADELVDTKSLER
                     RYFVETQFDKILKQDQEIFRILGLGNHFQSNDLAYRYQIVTGYSPIKPQLIQDIIDNN
                     LLAGETQSSLNWNVINMLNAKYIIAPGMLNEANLTILDVNQQRKEVLYLNEGVLPRAY
                     FVSEVRFLPSEKDVVAFMNTTEFDPAKMALTSVALDTSAGFDTGGSVQVTDYTPNRVV
                     LNVETER"
BASE COUNT          622 a          455 c          532 g          761 t
ORIGIN      
        1 ctctggaatt tctacgagcc tattgagatt caaaccatca aaggcgcctg gcgcaaatgg
       61 cgtaatcctt taaattggaa aaagaaccgt tctaagatta aattagaccg taaataatga
      121 gggttgaatt atgagtaaaa aaaccaaaaa gtcaacatct gccaaaaatg tccctccgca
      181 gaataagaag ccggttaact ggctagctac gattaatccc cgaaaggcga caatcttagc
      241 atttcttatc gtattattga tattaattat tttttataag ccctatgtaa ttgatcgtct
      301 tgaaccggct ggtggcgatc ggatggcaag cattgcccag agtaaattta tcaatgatta
      361 tcgtgaacaa actggtgaaa ttccactctg gaatcccgcc attttttgtg gagtaccgat
      421 ttattatgct ctctggtcta attcattcca tctggattat ttaatcagta aaattagtaa
      481 ggtaattgat tggaaattga gctggtttat tttggcgatg gtgggaatgc ttttaatatt
      541 ttggttgttg gaattcccgt ggtattatgc cttgcttgga acgattgcct ttctctttta
      601 tccacattat caggcgctga tcaccgtcgg tcattttgcc aaagtgcgag cggtttgcgc
      661 gatgccgtta gccgtgtttg gctttttata tttagtcaag aagcgaaatt ttctgtcatt
      721 tttactgttt ttaatctttt tctcactcca attgcgtacc cagcattatc agatagtctt
      781 ttatacgcta ttagttttta tggctctggg catacggcaa attgttgaat gggtcaagac
      841 caaacaagcc cagaaaattt acgtttccct cggattattc gtagccggat tggtgacctc
      901 agttttaatg tcagcccagc cgttatttgt ggtgaatgaa tatacaccct attccactcg
      961 cggcggccaa gccattaatc taaaggaaga tgcgactcag gcggaagtaa agagtagcgg
     1021 cgtgactttc gaatatgcga cgcgctggtc gcttaacccc aaagaattag ctaccctgat
     1081 tgtaccgcgt ttttatggcg gcacatctca ggaaccctac accggcaaag cttatccgca
     1141 actgcggggt cagcctattc cgggttattg gggcgatatg ccgtttacac aatcatgtga
     1201 gtatatggga attttgatcg tgattctggc tttgctgggc ttgtggtatt atcgcaaaga
     1261 tggggtggta atcagtttat ttatcctgct ggtattttcg ctactgctgg gtttcggcag
     1321 tcattttcca attttataca aaccactttt caagtattta ccctatttta gcaagtttag
     1381 agttccatca atgattttga ttctggttga tttcattcta gtaattctct cggtttatgg
     1441 attaaaagga ctggtagaaa aatttgattt agccaaatat aaatcggctc taatggtcag
     1501 cggctttttt atattgttcg gtttattctt tttgctggcg ccgggagtgc tgtcgtacac
     1561 ttccgctcag gacgctcaat atatgaacaa tccccaagtc attgaaatgt tgcgcacggc
     1621 gcggcgtgaa tttttacagg cggatactct acggatgctg gtttttgtaa tttgtttcgt
     1681 agctctgctg gtgctatata aaatgaagaa gattcgcccg gattttttaa ttggcgcgat
     1741 tgtcattctg gtcgcagtag atatgattaa tgtcagtaag cgtttcctga gagctgacga
     1801 attagtggat accaaatccc tcgagcgacg ttattttgtc gaaacccagt tcgataaaat
     1861 tctcaaacag gatcaggaga tttttcggat tctaggattg ggtaatcact ttcaaagcaa
     1921 tgatttagcc tatcgctatc aaatcgttac gggctattca ccgattaagc cgcaattgat
     1981 tcaagatata attgataaca atttattggc gggagagact cagagctcgt tgaactggaa
     2041 tgtgatcaat atgctgaatg ccaaatatat tattgcgccc ggcatgttga atgaagctaa
     2101 tttgacgatt ttggatgtta atcagcaacg gaaagaagtg ctttatttaa atgaaggcgt
     2161 ccttccgcgg gcttatttcg tatctgaagt caggttttta ccgtcggaaa aggatgtggt
     2221 agcttttatg aacacgaccg agttcgatcc ggctaaaatg gctttgacga gtgtagcgct
     2281 tgatacttcc gcaggatttg atactggagg aagcgttcaa gtaaccgatt atactcccaa
     2341 tcgcgtggtg ctaaacgtcg aaaccgagcg
//
LOCUS       DAOOMU010000169         2369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_55749_length_2369_cov_3.632671, whole genome shotgun sequence.
ACCESSION   DAOOMU010000169 DAOOMU010000000
VERSION     DAOOMU010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55749_length_2369_cov_3.632671"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="PLI55_09855"
     CDS             <1..1198
                     /locus_tag="PLI55_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB01175.1"
                     /translation="TMNARTQAMFDDNETYPYLVEGSWLRKCPAFTAPESLLTTQVDE
                     LKTFSVSTVDEASGAILPVWRVVNTDLDNDYIYSDWPIPVDLSYTDKDLLTAAYGRFP
                     VGDLNWFPDKKAAWAAQRDAEITAIEALRNSGKIDIATNGGFEQTPAGAVTDLAKGVA
                     GWVIEVAASIDPKPAFEIVADPVQEGGHALAITVNVLGTNAWDIQVVAEDIPVKPGAT
                     YIYSIWAKASAAAKANFTVGNADYSEYMRVHEKALTTEWTEIRKEFTVNDQYTVIRAP
                     IHFNITGNAGVTFYIDNLTITEKPSAIAPTTKAEKFALRQNYPNPFNPTTTINFTLPE
                     PGNVTLKVYNMLGQEVATLINNEFRGANGNHSVLFDGSRLSSGVYFYTITYGNKTVSK
                     KMVLMK"
     gene            1429..>2369
                     /locus_tag="PLI55_09860"
     CDS             1429..>2369
                     /locus_tag="PLI55_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPB01176.1"
                     /translation="MKSNFYILILLLAMTTNIFTNCGKQTELAQKTIAVIDDHYKISF
                     ADLHKYVYDEYFHRRYRDKSEAYRRALDEMVLDQLKCIDFFKRGLNNDPQLMQQLQRY
                     INEELIVKYFESEYLGKYTSEEFARKTYDQMGKEVYYQQIILNKPENASDAQLDALKN
                     KAVEIKTKAEQGVDFNELIKQYATNNAPDVSVLTIDWEKSMVSPHNRAIFSLNKGDVR
                     ILDEYNAYYVVKITDVRPIKLKPFNKVKDELISQLQESYYYTSLDEYDRDKKALVNGD
                     SIVWNEKALDQLVKWSRIPNFYKAIYWDTLQSAISNGR"
BASE COUNT          755 a          442 c          493 g          679 t
ORIGIN      
        1 tacaatgaat gccagaactc aggctatgtt tgatgataat gaaacttatc cctatctggt
       61 cgaaggatct tggttgagaa aatgtcctgc ttttaccgct cctgaaagtc tgctaacaac
      121 tcaggttgat gaattaaaga cctttagcgt tagtacagtt gatgaagcca gtggtgctat
      181 cctacctgtc tggagagtag taaatacgga tcttgataat gattatattt attcagactg
      241 gccaattccg gtggatcttt cctatactga taaagacctc ttgacagctg cctatggcag
      301 gttcccagtt ggtgacttga actggttccc agataaaaaa gccgcctggg ccgctcaaag
      361 agatgctgaa attacggcta ttgaagcatt aagaaatagt ggtaagattg acatcgctac
      421 caatggtggt tttgaacaga caccggcagg tgctgtgacc gatttagcca aaggagttgc
      481 cggctgggtt attgaagtag ctgccagtat tgatccgaaa ccggcttttg agatcgttgc
      541 cgacccggta caggaaggtg gacatgctct cgctatcact gtcaatgtcc ttggaactaa
      601 tgcctgggat attcaggtag ttgccgagga tattccggta aaaccaggtg ctacttatat
      661 ctattcaata tgggcgaaag cctcagccgc agctaaggct aatttcacgg ttggtaatgc
      721 tgattatagt gaatatatga gagttcatga aaaagctctc actactgaat ggacagaaat
      781 tcgcaaagag tttaccgtca atgatcagta cacggtaatc agagcaccta tccacttcaa
      841 cataactgga aatgcgggag ttacgtttta tattgataac ctgacaatca cagagaaacc
      901 ttctgctatt gcaccgacta cgaaagcgga aaaattcgcc ttacgccaga actatccgaa
      961 cccgtttaac cctaccacga ctataaactt tactcttccg gaaccgggca atgttactct
     1021 gaaagtttat aatatgttgg gacaggaagt tgccacattg attaataatg aattccgcgg
     1081 agctaatgga aaccacagtg ttttatttga tggttccaga ctttccagcg gcgtctattt
     1141 ctatactatc acctatggta acaaaacagt atccaaaaag atggtattga tgaagtaggc
     1201 atttccatta ttcgattaat gaatgactta tgcaaaaccc cggtattttc tgccggggtt
     1261 ttgcataaaa aaacgacgat actcaaattc attaatgact tggaaaatat aaaactccta
     1321 tttttaggga actaactatt actacttgtc tgtttaatgg tcgtgaattt accggtcagt
     1381 tttgttaaat ttcaatctag aaaattttcc aattaagtga ggtttattat gaaatcaaat
     1441 ttttatatac taattctgct acttgcgatg acgactaaca tttttacaaa ttgcggtaaa
     1501 caaactgagc tggctcaaaa gaccatagca gtaattgacg accattataa aattagtttc
     1561 gccgatctgc ataaatatgt ctatgatgaa tattttcatc gaagataccg cgataagtcc
     1621 gaggcttacc gacgagcgct ggacgaaatg gtattggatc agctgaagtg tattgacttt
     1681 ttcaaaaggg gtttgaataa tgatccgcaa ttgatgcaac agcttcagcg atatattaat
     1741 gaagaattaa tcgttaaata tttcgaatca gaatatcttg gtaaatatac cagtgaagag
     1801 ttcgcccgga agacctacga tcagatgggt aaagaggttt actatcaaca aataatttta
     1861 aataaaccgg aaaacgcttc ggacgctcaa ttggatgctt tgaagaacaa ggctgtagaa
     1921 ataaaaacca aagccgagca aggggtagat tttaatgaat taattaagca atatgctacc
     1981 aataacgccc cggatgtttc tgttcttacc attgactggg aaaaaagtat ggtgagtcct
     2041 cataaccggg ctatttttag tcttaataag ggtgatgtcc gaatactgga cgaatataat
     2101 gcttattatg tggtcaaaat taccgatgta agaccaataa aactgaaacc ttttaataaa
     2161 gttaaagatg agttaatctc tcaactccaa gaaagttact actataccag cttggacgaa
     2221 tatgatcgcg ataaaaaagc actagtcaat ggggattcga tcgtatggaa tgaaaaagcg
     2281 cttgatcagc tagttaaatg gtcaagaatt cccaacttct acaaagctat ttattgggac
     2341 accttacaaa gtgcgatttc gaacggtcg
//
LOCUS       DAOOMU010000170         2301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_58136_length_2301_cov_9.064559, whole genome shotgun sequence.
ACCESSION   DAOOMU010000170 DAOOMU010000000
VERSION     DAOOMU010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58136_length_2301_cov_9.064559"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..2098
                     /locus_tag="PLI55_09865"
     CDS             230..2098
                     /locus_tag="PLI55_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HPB01177.1"
                     /translation="MLFSQLYPQVLRINGNVTSSTMPVQNAVVTFENAFDPSCRFSTL
                     TDSTGYYQLEVINSAITPPYRIPTKFDLGQNYPNPFSATTAIPYSLKQDSRVFLTIYD
                     LLGREVRKIEAGVQPVGSYQVLWDGCNNHGQKVATGLYFYRLEAGGESRIRKLIYDGS
                     GLHQSATTTPQSVQMSKIDLAKRVSLPGQNFTVKLDNCDSTFPVIIPIQIENVYLEND
                     TTINFTVSSVPVATVYPDSLKQYIRGFGAANILPWRPDMTDAEIEKAFGTGDGQIGFS
                     MLRLRLPSEETTASFQTQVATAKKAYDRGVLIFASPWSPPSRMKSNNSTIDGYLLESH
                     HQDYVDHLNSFIDYMADNGVDLYAVSVQNEPDVDVSYESCDWSPEQMRKFMAENAGTI
                     KTRVIAPESYQFRREMSDPILNDSAACANLDIVGGHIYGGGLGAYPLAEQKGKEIWMT
                     EHLTGEKSHSNDWTWAFDVAKEMHSCMEAGFSAYVWWYIVRYYGPISDGEKESSGGGR
                     KGEVTKKGYVMSQYSRFIRPGYHRIKCNSMPQKSVLATAYKDSASSKMVLVVLNISSS
                     STLQQTFTIPNGNVASFTPFTTTSTKNCQQAGNIPVINNRITVTLEPSSITTFVSD"
BASE COUNT          670 a          433 c          536 g          662 t
ORIGIN      
        1 gagatttggg gcgataaaat acctaatccg gtcgcggtga gatatgcctg ggctgataat
       61 cccgcgggag ccaatttgta taataaaggc ggtttaccag cctcaccttt ccgcaccgat
      121 gatttttaat aatttaattg gaatttcaat ggagtttaaa ttgcttttaa aaagccgtac
      181 taaaaggagt gcaaatcatg ttttaggggc tttggttctg aattcgataa tgttattttc
      241 acagttatat cctcaagtat tgcgaatcaa cggtaatgta accagttcga ctatgccagt
      301 tcaaaatgca gtagtaacct ttgaaaatgc ttttgatcct tcctgccgat tctcgacttt
      361 gaccgattct acgggctatt atcagctgga agtgataaat agcgcgataa ccccacctta
      421 tagaatacct accaaatttg atctgggaca gaattatccc aatccctttt cagccacgac
      481 tgccattcct tattctctga agcaagattc cagagttttt ttgactatct atgatctact
      541 tgggcgagaa gtacgtaaaa ttgaagccgg agttcagcca gtagggagtt accaggttct
      601 atgggacggt tgtaataatc atggtcagaa agtagcgacg ggattatatt tttaccggct
      661 ggaagcgggc ggtgaatcca gaatccgcaa actgatctat gatggaagcg gattacacca
      721 atcagcaacc acgacgcccc aatcggttca gatgtcaaaa attgatctgg caaaaagggt
      781 gagtctgccg ggacagaatt ttacggtaaa acttgataat tgtgacagca cctttcctgt
      841 aattatacca atacagattg aaaatgtgta tctcgaaaat gacacgacta tcaattttac
      901 tgtatcttca gttccagttg ctactgttta tccggatagt ctgaagcagt atattcgagg
      961 ttttggggcg gctaatattt taccctggcg gccggatatg accgatgctg aaatcgaaaa
     1021 ggcttttggc acaggcgacg ggcaaattgg tttctcgatg ttaagattga gactgccttc
     1081 agaagaaacc accgcgtctt ttcaaacgca agtggcgaca gcgaaaaaag cttatgaccg
     1141 gggagttttg atttttgctt caccctggtc gccaccatct agaatgaaaa gtaataacag
     1201 cactattgac ggttaccttc tggaatctca tcatcaggat tatgtcgatc atttaaattc
     1261 ttttattgat tatatggctg ataatggtgt tgatttatac gcggtctctg ttcagaacga
     1321 accggatgtt gatgtatctt acgaatcttg tgactggagt ccggaacaga tgcggaaatt
     1381 tatggcggaa aatgctggaa ctattaaaac cagagtcatt gcgccggaat cctaccagtt
     1441 caggcgagaa atgtcagacc caatattgaa tgactcggcg gcttgcgcta atctggatat
     1501 tgttggtggt catatttacg gcggtggttt aggggcttat ccgctggcag aacaaaaagg
     1561 caaggaaatt tggatgaccg agcatttaac cggagaaaaa agtcactcca atgattggac
     1621 ttgggcattt gacgtggcta aggaaatgca tagttgtatg gaggctggat tcagcgctta
     1681 tgtctggtgg tatattgttc gatattacgg acccattagc gatggtgaga aagaaagtag
     1741 cggaggcggt cgcaaaggtg aggttactaa aaaaggctat gtgatgtcgc aatattcgcg
     1801 ctttattcgc ccgggatacc atcggatcaa atgtaattct atgcctcaaa aatcggtatt
     1861 ggcgacggct tataaagata gtgcctcgtc caaaatggtg ttagtagtgc tgaatatcag
     1921 ttcgtcttcg actcttcagc aaactttcac gattccgaac ggaaacgtgg cgtcatttac
     1981 accatttacg actaccagta cgaagaactg ccaacaagcc ggtaatattc cggtaattaa
     2041 caataggatt acggttacgc tggaaccgtc aagtataacg acatttgtat cggattaatg
     2101 tgttatgtgt aaatttaatc tgtaaatgta accctaaata aaatgagagt gaaaactgat
     2161 atttcaagtc gtataattaa tccactttta acttagaaag gagtacgtca aaagtgagaa
     2221 aatggatcgt attagcactg ttgctgatat catttcttgg ggctaatgtt ttagcgcagg
     2281 aaatgccgtt ggtatatgaa g
//
LOCUS       DAOOMU010000171         2254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_59920_length_2254_cov_19.115962, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000171 DAOOMU010000000
VERSION     DAOOMU010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59920_length_2254_cov_19.115962"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="PLI55_09870"
     CDS             <1..585
                     /locus_tag="PLI55_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPB01178.1"
                     /translation="ANASGFYSFLNEKSPDLVILDLMLPDIDGVEICRQLRSNPQTAK
                     IPIIMLTARSEEFDKVLGLEIGADDYVTKPFSTRELVARVKAVLRRSQPTLSENILKI
                     TDSLTIDLEKYIVQADGKPIALTATEFNLLKILAQKPGHVFSREHLLDQLWGEDKIVV
                     NRTIDVHIKNLREKLGEYGSLIRNLRGVGYKLEI"
     gene            582..>2254
                     /locus_tag="PLI55_09875"
     CDS             582..>2254
                     /locus_tag="PLI55_09875"
                     /inference="COORDINATES: protein motif:HMM:NF028046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB01179.1"
                     /translation="MKKSFFYRIFSGYLILIVALTVLIVALSYNSLRKQFVEAQIANL
                     QDLNTSLSVLLLSAKNQSHEDLDHLTKSLGNEIRARITIIDTTGNVIADSESDPRLMK
                     NHRQRPEIKQALRGNVAHSQRFSTTVEENMLYVASPLVQDGQTVAVVRTSIYLSHLND
                     IFKQILNRIIKIAILIILIALSAAFLLARYYSKPVNQLVTAARQIGQGKLETRVNIQT
                     SDEFAELADSFNAMAVEINRLLADVARQRDELQTIVAAVPSGLLVLTADGRVAFYNRE
                     FQKITEREELQDRFYWEILRQPQFTEFIKDIQASRANRTQEIVINNKTYLCSAEAETS
                     SAEIVLIFSDISELKAVQQMKRDFVVNVSHELRTPLTAIKGFVETLQEETSGDERHYL
                     TIIARHTDRLINIVNDLLTLAEIEEKNQLEYELVNISNLINDTVKIFEERLQAKNLKM
                     IVEIADGLTTIKVDPFKFQQVLINLLDNAIKYTEQGQITIRVQKKADQVQIVIQDTGC
                     GIPPEHLPRLFERFYVVDKARSRQMGGTGLGLAIARHIVLLHNGTIEVE"
BASE COUNT          655 a          464 c          521 g          614 t
ORIGIN      
        1 gccaatgcca gtggatttta tagctttcta aatgaaaaat cgcctgactt ggtcattctc
       61 gacctaatgt tgccggacat tgatggggtg gaaatctgta ggcaactgag aagcaatcct
      121 caaacggcta aaattccgat tattatgttg actgctcgca gtgaagaatt cgacaaggtt
      181 ctcggattgg aaatcggtgc cgacgattat gtcactaagc cattttcgac gcgagaatta
      241 gttgcgcgag tgaaagccgt tttacggcgc tcgcaaccga ccttaagtga gaatattttg
      301 aaaatcaccg acagtttaac aattgatctg gagaaatata tcgttcaggc tgatggcaaa
      361 ccgattgcat taaccgccac tgagtttaat ctcctgaaaa tattggcgca aaaaccaggg
      421 cacgtttttt cacgggagca tctgcttgat caactttggg gtgaagataa aattgtcgtc
      481 aaccgcacta ttgacgttca tatcaaaaac ttacgggaaa agctgggcga atacggtagc
      541 ctgatccgca atttaagagg agttggttac aagctcgaaa tatgaaaaaa tctttttttt
      601 accgaatatt ttccggctac cttattctga ttgtagcgct aacggtctta attgtagcac
      661 tttcatataa ttcgctccgc aagcaattcg ttgaagccca gatcgctaac ctgcaggatt
      721 tgaataccag tctgtcggtt ttattgttat cggctaaaaa tcaaagccat gaggatttgg
      781 atcatctgac caagtcgctt gggaatgaaa tccgggctcg catcacaatt attgatacca
      841 ccggtaatgt cattgccgat tctgagtctg atccgcgcct gatgaaaaac catcgccagc
      901 gaccggaaat caagcaggct ttgaggggta atgtggcgca ttctcaacgt tttagcacga
      961 cggttgaaga aaatatgctg tatgtcgctt caccgctggt tcaagatgga cagactgtag
     1021 cagtcgtgcg tacgagcatt tatttgagcc atttgaacga tattttcaag caaattctca
     1081 atcgaattat caaaattgct atacttataa ttttaatcgc gctgagcgcg gcttttctgc
     1141 tggcgcgtta ttattccaaa ccggtcaatc aattggtcac agccgctcgt caaattgggc
     1201 agggcaaact cgaaacccgt gttaatattc agaccagtga tgaatttgcc gaactggctg
     1261 atagttttaa tgcgatggcg gttgagatta atcgcctgct ggcggatgtt gcccgacaac
     1321 gtgatgaatt gcagacaatc gttgcggcgg tgccttccgg tttactggta ctgactgccg
     1381 atgggcgggt ggcattttat aatcgtgaat ttcaaaaaat tactgaacgg gaggaactac
     1441 aggacaggtt ctactgggaa attttacgtc agccacagtt taccgagttt atcaaagata
     1501 tacaggcaag tcgagctaat cgcacccagg aaatagttat aaataataaa acctatttgt
     1561 gcagtgctga ggcggagacg tccagcgctg aaattgttct gatattttct gatatttcag
     1621 aactgaaagc cgttcaacaa atgaagcgtg acttcgtggt caatgtctcg cacgaattgc
     1681 gcacgccatt gacagccatc aaaggctttg tcgagacact tcaagaagaa acatccggcg
     1741 atgagcgtca ctacctcaca attattgcgc ggcataccga tcgcttgatc aatattgtca
     1801 atgacttgct gacactggcg gaaatagaag aaaaaaatca gctggaatat gagctcgtta
     1861 atatatcgaa cttgattaat gataccgtta aaatttttga agaacgcctg caagcgaaaa
     1921 atctaaaaat gatagtcgaa atcgcagatg gcttgacaac aattaaagtt gatccattca
     1981 aatttcagca ggttttaatt aatcttctgg ataatgccat aaaatacacc gaacagggac
     2041 aaatcactat tcgggtgcaa aagaaggcag accaggtcca aattgttatt caggataccg
     2101 gttgcggtat tccaccggaa catctaccgc ggcttttcga gcggttttac gtggtggata
     2161 aagcccgctc gcgtcagatg ggcggaaccg gtttgggatt agcaattgcc agacatattg
     2221 ttttacttca taatggtacg atcgaagtcg agag
//
LOCUS       DAOOMU010000172         2221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_61189_length_2221_cov_7.864728, whole genome shotgun sequence.
ACCESSION   DAOOMU010000172 DAOOMU010000000
VERSION     DAOOMU010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61189_length_2221_cov_7.864728"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLI55_09880"
     CDS             <1..518
                     /locus_tag="PLI55_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01180.1"
                     /translation="GALYAQIADFSGERLTFTIHSQFIEMTGIYYFTNRTGQEIKLPI
                     IYPFQVNSHQAFPDSIAVSLGNGSPLHFEIRKESIYFSVPLLNKEQNALEIYFRQPIT
                     RPQFEYILTSTSSWQKPLEFADFTIRVPLAYRLANLSFPYEKIDTTDDFQVYHLHFEN
                     FYPEQNLIFSW"
     gene            575..2122
                     /locus_tag="PLI55_09885"
     CDS             575..2122
                     /locus_tag="PLI55_09885"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB01181.1"
                     /translation="MKKSPAILILFWATVGLANPIAAQLFSELYFDSTGWKIEVMGDW
                     YGENNERIVIVSLTDSAVTDTSFHLSGEFPVITPADMETDLYINPAGDYLKIGILNEY
                     GLYFFNNLIFGENGVIAAPRAGESIAFGGFIEASELMYYLDNTPTLGAINDCGNSKGT
                     IQGIVTDSSGMPIPDVKVKYSVTCSSESEDMSAVTDANGRFEFECLTAQQIFSFQHDH
                     YEFLKVPVQIWPDSTIEKTFVLTAFPPNYQNYFPLQDGNYWNYYSYIEDSYSEHISIN
                     ISATILINDKTYYLMGNDTIGYDSLKNVVIYKNQRDSLLYPLSLSDGNSMITPDSLWM
                     MKLNYDQTPITVRVGTFTDNIIVEKTFIGVRKSGLPPDTILTYFSKNVGIIKNINICH
                     SLISKSVERKELYHAYVNGTLYYENFYQVEQSFTPIKYNLSLTNYPNPFNSSTTIKYH
                     LAETGDVRLSVFDLSGRLVAELFKGRQPAGDYHYLWSGDNLPSGVYLLSLQAGGKRVI
                     QKCLLMK"
BASE COUNT          650 a          449 c          429 g          693 t
ORIGIN      
        1 ccggtgcgct atacgcccag atcgccgatt tcagcggcga gcggttgact tttactatac
       61 actctcaatt cattgagatg accggcattt actattttac aaaccgcact ggacaggaaa
      121 tcaaactgcc gattatctac ccgtttcagg tcaattctca tcaagctttt cccgactcaa
      181 ttgcagtcag tttgggaaat ggcagtccct tgcattttga gattcgcaag gaaagtatct
      241 atttttcagt acctttattg aataaggaac aaaacgcgct tgagatttac ttccggcaac
      301 cgattactcg gccacaattt gagtatattc tcacctcgac cagcagttgg cagaaaccgt
      361 tggaatttgc cgattttacg attcgggtac cgcttgccta tcgtctggca aatctgtcat
      421 ttccgtatga aaaaattgac actaccgacg attttcaggt ttaccatttg cattttgaga
      481 atttctatcc ggagcagaat ttaattttta gttggtaggt taaaagcaca aaaacacaag
      541 gacttatgaa acctttaata atggagtaaa aactatgaaa aaaagtcctg cgatattgat
      601 tttattttgg gcgactgtgg gattagccaa tccaattgca gctcaattat tttcggaatt
      661 gtattttgat tctaccggct ggaagattga agttatgggt gactggtatg gcgaaaataa
      721 cgaacgtatc gtgatcgtgt ctttaactga ttcggcagtt acggatacgt ctttccatct
      781 ttcaggtgaa tttccggtta ttactccagc cgatatggaa acagaccttt atatcaatcc
      841 ggctggtgat tacttgaaaa ttggaatact taatgagtat ggattgtatt tcttcaacaa
      901 tttaattttc ggtgagaatg gtgtcattgc cgctccacgt gccggagaat ctattgcctt
      961 tggaggtttt atagaagctt cagaattgat gtactacttg gataataccc cgactctcgg
     1021 tgctattaat gactgcggaa attcaaaagg tactattcaa ggaattgtca ccgacagtag
     1081 cggcatgccg attcccgacg taaaggtgaa atattcagtt acttgcagtt ccgaatctga
     1141 agatatgtcg gcagttaccg acgcgaacgg ccggttcgaa ttcgaatgcc tgactgccca
     1201 acagatattt agtttccagc acgatcatta tgaatttcta aaagttccgg ttcaaatctg
     1261 gccggacagt accattgaaa aaacatttgt cttaaccgcc tttccgccta attatcaaaa
     1321 ttattttcct ttacaagatg gtaattactg gaattattat agttatattg aagactcata
     1381 cagcgaacac atttctatta acatctccgc gactatcttg attaacgata aaacatacta
     1441 cctaatggga aatgatacga taggttatga tagtcttaaa aatgtcgtga tctataaaaa
     1501 tcagcgtgat tcacttttgt atccactttc tttatcggat ggcaattcaa tgatcactcc
     1561 cgattctctc tggatgatga aattaaatta cgaccagact ccgattaccg ttagagtcgg
     1621 aacattcacc gacaatataa tcgttgaaaa aacgtttata ggagtaagaa agagtggact
     1681 accacccgat accattctaa cttatttctc gaaaaatgtt ggtattatca aaaatattaa
     1741 tatttgtcac tctcttattt cgaaatccgt tgagagaaaa gaactatatc atgcctatgt
     1801 aaacggcacc ttatattatg aaaactttta ccaagtcgaa caatctttta ctccgataaa
     1861 atacaacctg agtttgacca actatcctaa tcccttcaac tcctcaacta ctattaaata
     1921 tcatctcgcc gaaaccggcg acgtccggct gagcgttttc gacctgagtg gacgattggt
     1981 agcagagctt tttaaaggtc gtcaaccagc tggcgattat cattatctct ggtcgggtga
     2041 caatctgccg tcgggtgtgt atcttcttag tctgcaagcg ggtggtaagc gcgtaattca
     2101 gaaatgtcta ctaatgaaat aacaattctg gcttcggaaa gtttacttta ccataaaata
     2161 taatttatat aatttagcgt gccgtcctaa tcggcagatt ggtttttgca acttgtttta
     2221 g
//
LOCUS       DAOOMU010000173         2212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_61553_length_2212_cov_21.866481, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000173 DAOOMU010000000
VERSION     DAOOMU010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61553_length_2212_cov_21.866481"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLI55_09890"
     CDS             <1..298
                     /locus_tag="PLI55_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01182.1"
                     /translation="IEKTDKIMSSKTDKVDFSKNLSRHEKEMGIQISEMEKYKWICSK
                     DQGRDVGKMAYFEWTQKYGHKVRDWLESLSDEEIDRLFAELSDQVKNYIEEKSH"
     gene            360..>2212
                     /locus_tag="PLI55_09895"
     CDS             360..>2212
                     /locus_tag="PLI55_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HPB01183.1"
                     /translation="MESKDSTVTKLPENAYRPLKPGEEYIPVIPDAKIVPEVTPYSLG
                     WGLFYAVLFSMAAAYLGLKIGQVFEAAIPIAILAAGTSVLLKRKNALQENVIIQSIGS
                     SSGVIVAGAIFTIPGLYILGLDASYLQIFLASLLGGFLGILFLIPFRKYFVKDMHGEF
                     PFPEATATTEVLIAGEGGGAQAKVLVSAAIVGGLYDFCVAGFGFWKEVVSTSVFRWGA
                     LLADKLKWVLKINIGAAVTGLGYIVGLRYSAIIASGSFLAWWVIVPIVSYVAGLGAQT
                     PDAIFFGYVRPIGIGAIAAAGIIGILKSSKIIGGAFKLAGKELFGGSKGGTVEETSRT
                     QKDIPMKWVTVFLVLTLISIFFFFLFGVRITLKQAIVGLLIVAVISFLFTTVAARAIA
                     IVGTNPVSGMTLMTLLLSSFILVKVGLTGDHGMLAALLIGGVVCTALSMAGGFITDLK
                     IGYWLGTTPIKQESYKFLGSLFSAASVGLVILLLNKTFGFGGDSPLQAPQASAMAAVI
                     KPLMTGEQAPWVLYLAGVFLAIILEIIRVPSLAFALGIYLPIQLNTPLLFGGLVAYLV
                     SKSTKNETLAKARSDRGTLIASGFIAGGAIMGVVSAFIIFIGQNIFGDGWTL"
BASE COUNT          504 a          471 c          575 g          662 t
ORIGIN      
        1 catcgaaaaa acagataaaa ttatgtcctc taaaaccgat aaggtagatt tcagcaaaaa
       61 tctctcacgt catgagaagg aaatgggcat ccagatttcc gagatggaaa agtacaaatg
      121 gatatgtagt aaggatcagg gacgtgacgt cggtaaaatg gcttatttcg aatggactca
      181 aaaatacggt cacaaagtcc gcgactggtt ggagtcttta tcagatgaag agattgatcg
      241 gcttttcgcc gagttatccg accaggtcaa gaattatatt gaggaaaaat cacattagcc
      301 gatctttatg atcgcatcgc aatccacata cacttaacca taaaaatagc gggggaataa
      361 tggagtcaaa ggattcgact gttaccaaat taccggaaaa tgcttatcgt cctctcaagc
      421 cgggtgaaga atatatacca gtaattcccg atgcaaagat cgtcccggaa gttactccct
      481 actcgctcgg gtggggactt ttttacgctg tgttattttc gatggcggcg gcatatttgg
      541 gactaaaaat cggtcaggta ttcgaggcgg ctattccgat tgccattctg gcggctggga
      601 catcggttct tttaaagcgt aaaaatgccc tgcaggaaaa cgttattatt cagtccatcg
      661 gttccagttc aggggtaatc gtggcgggag cgattttcac aattccgggg ctttatatac
      721 tcggcttaga tgccagctat cttcagattt tcttagcgtc tcttttgggt ggctttctgg
      781 gaattctatt tctgattcct ttccgtaaat atttcgtcaa ggatatgcac ggcgaatttc
      841 ccttcccgga agcgacggcg acgacggaag tgctgattgc cggagagggc ggtggcgctc
      901 aggcaaaagt gctggtatcc gcggcgattg tcggtggact atatgacttt tgcgtagctg
      961 gattcggttt ttggaaagaa gtggtctcga cctcggtttt tcgctggggg gctttgctgg
     1021 ctgataaatt aaagtgggtt ttaaagatta atatcggtgc ggcggtaacc ggtctggggt
     1081 atattgtggg tctgcgctat tcagcgatca tcgccagcgg ttcattttta gcctggtggg
     1141 taatcgtgcc gatagtgagt tatgtcgccg gacttggtgc ccaaactccg gatgcgattt
     1201 tcttcggtta tgtgcggccg attggaattg gggcaatcgc ggccgccggt attatcggta
     1261 ttctaaaatc ttctaaaatc atcggcggcg ctttcaaatt agccggtaaa gaactattcg
     1321 gcggctctaa gggcggtact gttgaagaaa cttcccgcac ccagaaagac atcccgatga
     1381 aatgggtcac ggtttttttg gttctgacat tgatctccat ctttttcttc tttttattcg
     1441 gtgtccggat tacgctgaaa caggcgatcg tcggcttact aatcgtggcg gtgatctcat
     1501 ttctattcac tacggttgcc gcccgggcga ttgccattgt cggtactaac ccggtttccg
     1561 gtatgactct tatgacgctt ttgttatcat catttatttt agtaaaagtc ggactgaccg
     1621 gcgatcatgg catgctggcg gccctcttga tcggcggggt ggtgtgcacg gctttatcta
     1681 tggcgggtgg ctttatcact gacttgaaga ttggttactg gctcggtact acgccaatca
     1741 agcaggagag ttataaattc ctcggttcgc ttttttcggc ggcttcggtc ggattggtaa
     1801 ttttattact taataaaact tttggttttg gtggcgattc accgcttcaa gccccacagg
     1861 cttccgcgat ggcggccgtt attaaaccat tgatgaccgg ggagcaagca ccttgggttc
     1921 tttatttagc cggtgtattt ttggcaatta tcctggaaat tattcgggtg ccgtcgttag
     1981 ctttcgctct cggaatttat ttgccgattc agcttaatac acccctgctg ttcggcggct
     2041 tggttgccta tttagtttct aaaagcacaa agaatgaaac cctcgccaag gcgcgtagtg
     2101 accgcggtac tctcatcgct tccggattta ttgctggtgg cgccattatg ggagtcgttt
     2161 cagcctttat catttttatt gggcaaaata ttttcggcga cggctggacg ct
//
LOCUS       DAOOMU010000174         2193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_62344_length_2193_cov_12.237605, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000174 DAOOMU010000000
VERSION     DAOOMU010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62344_length_2193_cov_12.237605"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2129)
                     /locus_tag="PLI55_09900"
     CDS             complement(<1..2129)
                     /locus_tag="PLI55_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPB01184.1"
                     /translation="MSFIFGQSPSFQTYINPVIPGDHPDATLTRIGNDFYTTGSSFNV
                     TPVIYHSTDLVHWQAIAQPVSAAWSNYGDQAGGGCWGGQMVYYNGEYWHFFSRSNTMY
                     YVKASAPEGPWSMPVRINNPSTLPYGLGYDNSIFIDDDHKWYLVVKNGQPNNGIVELG
                     NDGQPTGVVYNLNWLNPGPDYPYSWAEGPVMWKYNGYYYYSFARDLGGGQKVMRSRTL
                     TADQSAWEMLGDFFNENDPQKGSSLFTSPNHASAAVMLDDSTSWVIHPLYAKGEWKGQ
                     GRQGLLNQVHYDANGKPVADYPVNKSFTAPKLPSSGIPWMVPHSDFFTSSELHPEWSF
                     LGYTPAVKYSLTDRPGWLRLSPKDKALANTVIKNDGEHNYSLITRLDFNAQSTTDQAG
                     LWIMRGDQTMEVKLVSTLSTEGFKVITYSFKSTIYETENTIGDTLWLKLVRINHKISG
                     YFSGDGFDWIEIGAPIDISEIDSYSDFSTFTGTRQGLYVKGMSDAWFDFYIYRDAYTP
                     IMAECPANQFGTALVLNKYPPNSLGKIHNDDWALYAGVEFGNTEYAKNCDSLKITASC
                     LIAGSAVEVYLDSIDVNTKIAVCAIDTTGSWTNFQTFTTDLLNPISGNHDVYLKFIGP
                     GSGELFRLQSFQFITNSKPTAINSCPENNLPRRFYLEQNYPNPFNPVTRIKYSVPKTS
                     YLTIKVYDVMGREVATVFDGVQQAGNYT"
BASE COUNT          620 a          489 c          483 g          601 t
ORIGIN      
        1 gtataatttc ccgcctgctg aacgccatcg aataccgtgg ctacctcccg ccccatcaca
       61 tcataaacct tgatggtcag gtaactggtt ttcggcactg aatattttat tcgagttacc
      121 ggattgaaag gattaggata attctgttca agataaaatc ttcgcggtag attattttcc
      181 gggcaactat tgattgcggt gggtttggaa ttggtaataa attggaacga ttggagtcga
      241 aataattccc cacttccagg accaatgaat tttaaatata cgtcatgatt tccagatatc
      301 gggttcaaca aatcagtggt aaaagtctga aaatttgtcc atgagccggt cgtgtcaatg
      361 gcgcaaaccg caattttagt atttacatct atcgaatcaa ggtaaacttc caccgcgctt
      421 ccggcaataa ggcatgaagc ggttattttt agtgaatcac aatttttcgc atattcagta
      481 ttgccgaact caaccccggc gtacaatgcc caatcatcgt tgtggatttt gcccagtgaa
      541 tttggcgggt atttattcaa tactaacgca gtgccaaact gattcgccgg gcattcagcc
      601 attatcggcg tataagcatc gcgataaata taaaaatcga accaggcatc gctcatccct
      661 ttcacgtaca ggccttggcg cgtaccggta aaggtcgaga aatcggagta ggaatctatt
      721 tccgaaatat ctataggggc gcctatttcg atccaatcga atccatcgcc gctaaaatat
      781 ccgctgattt tatgatttat acgaaccaat tttaaccaga gagtatcgcc gatggtgttt
      841 tcagtttcgt atatggtaga tttgaagcta taggttatca ccttgaagcc ttcagtcgat
      901 aaggtgctaa caagtttgac ttccatcgtt tgatcacctc tcataatcca gagaccggct
      961 tggtcggtag ttgattgagc attaaaatcc agtcgcgtaa ttagtgaata attatgttcc
     1021 ccgtcgtttt tgattaccgt gttagctagc gctttatctt tgggcgataa acgcaaccag
     1081 ccgggacgat cggtgagaga atatttaacg gcaggtgtat aacccaaaaa tgaccattcc
     1141 ggatgtaact cagacgaggt aaagaaatcc gagtgaggca ccatccaggg aataccgcta
     1201 ctcggcaatt tcggcgcagt aaacgattta ttaaccgggt aatcagccac tggtttgcca
     1261 ttcgcatcat aatgaacttg atttaacaat ccctgccgtc cctgaccttt ccattctccc
     1321 ttggcataga gtggatggat aacccacgaa gtactgtcat cgagcataac agccgctgaa
     1381 gcatgattgg gactggtgaa aagtgaggag cccttttggg gatcgttttc attgaaaaaa
     1441 tcgcccaaca tttcccaggc ggactgatcg gcagtcagag tgcgactgcg cataactttt
     1501 tgacctccgc caaggtcacg ggcaaatgaa tagtaatagt agccgttata tttccacatc
     1561 accggacctt ccgcccaact gtaaggatag tccgggcctg gatttaacca attgagatta
     1621 tagacgacgc cagtcggttg tccgtcattg ccgagctcaa cgatgccgtt attgggttgt
     1681 ccatttttca ctactaaata ccatttatgg tcatcatcaa taaaaatgga attatcgtat
     1741 cccaaaccat acgggagggt agatggatta ttgattctga ctggcatact ccagggacct
     1801 tccggcgcgg atgcttttac ataatacatc gtattcgacc gcgaaaagaa atgccagtat
     1861 tcaccgttat aataaaccat ttggccgccc cagcaaccgc caccggcttg atcgccataa
     1921 ttcgaccagg cggcgctcac cggctgggcg atggcttgcc aatgaactag atcggtagaa
     1981 tggtaaataa ccggagttac attaaatgac gaaccagtag tataaaaatc attgccgatg
     2041 cgggtcaaag tagcatcggg atggtcgcct ggaattaccg gatttatata ggtctggaaa
     2101 gacggcgact gcccaaaaat gaaactcatt ggaattaaac aaattaatat tttaggtaaa
     2161 aatcgcattg aagtcctcac atttaattta gtt
//
LOCUS       DAOOMU010000175         2184 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_62731_length_2184_cov_12.142320, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000175 DAOOMU010000000
VERSION     DAOOMU010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62731_length_2184_cov_12.142320"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="PLI55_09905"
     CDS             complement(<1..1066)
                     /locus_tag="PLI55_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HPB01185.1"
                     /translation="MTENKIPLGPFHPLLEEAEYFELKVDGETVVDIDMEIGWMHRGH
                     EFISESKTFTQSIYLVERICGICSTSHPLACVHAIEDIDNIEIPLRARYIRTLIGELE
                     RIHSHLLWLGLAGHFIGYDTLWMWAWKYREPILEMFEAITGNRNHYGMMRVGGVARDV
                     DIAIIPKLLKILDNVDRKMDMLAKAAHDDPVLKSRLKGVGVLTKADAIAYCAVGPVAR
                     ASGVAIDVRKDEPSDAYDLVEFDEIVRSNGDVYDKLVLRILEILESSKIVRQCLEKLK
                     VVTGPILNNVKEITPGEGIGRYEAPRGEVFHYIRSDGSNRPVRHKVRAPSYVNIPTYM
                     ASCKGIPVADALITLAAVDPC"
     gene            complement(1063..1494)
                     /locus_tag="PLI55_09910"
     CDS             complement(1063..1494)
                     /locus_tag="PLI55_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPB01186.1"
                     /translation="MDQFTQFMELMGDKVTQVIEKPRRLYFVVSNENLKEIARYLFVT
                     LGCRLSTATAQETYHGLEILYHFSHDASGCYYCPRVVLTDKKNPKVNTITDIVTGALW
                     IEREIFDLWGIEFVGHPRMEQLLTLNSPENPDRPLRFGRSL"
     gene            complement(1509..>2184)
                     /locus_tag="PLI55_09915"
     CDS             complement(1509..>2184)
                     /locus_tag="PLI55_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB01187.1"
                     /translation="PEKILSELESLLLTNKGNLVEIFIKDFISTEYIKYERFIEASNL
                     LDELLIYYEKYEMDEQAAYTLLNKTYLQQYLTNNLSIGKKGGIATQFKSVECLILLYS
                     KYPNSTAASLGKEELGDDFFINEPLPEQTEVIIADHRIYPNPFNINTTVAFDLPEEAG
                     VEIIVYDLMGREVWKSAKINYSAGTYSVIWHGVNQSGQPVGTGMYLVRLNSTKYNATQ
                     KVLLMK"
BASE COUNT          600 a          522 c          446 g          616 t
ORIGIN      
        1 agcacggatc cacggcggct aaagtaatta atgcatcggc aaccggaatc cccttgcagg
       61 aagccatata agtcggaata ttcacataac tgggggcacg cactttgtgt ctaaccggtc
      121 ggttactgcc atccgatcga atataatgaa aaacttcgcc gcgcggtgct tcatagcgac
      181 caattccttc acctggcgtt atttccttga cattattcag aatcggtcct gtgactacct
      241 ttaatttttc cagacattga cgaacaattt tactagattc cagaatttcc agaatacgta
      301 gcaccagttt atcgtaaacg tcaccattcg accttacaat ttcgtcaaat tcaaccagat
      361 catacgcatc actcggctcg tctttacgaa catcaatcgc caccccggat gcgcgcgcta
      421 ctggcccaac ggcacagtag gcaatcgcat cggctttggt aagcacaccg acgcctttca
      481 aacgcgactt caacaccgga tcgtcatgcg cggctttggc gagcatatcc atcttgcggt
      541 caacattatc cagaattttc agtaatttcg gtataatagc gatatcaaca tcgcgggcaa
      601 cgccgcccac gcgcatcata ccgtaatgat tgcggttacc ggtaatggct tcaaacattt
      661 ccagaatcgg ctcacggtat ttccaagccc acatccaaag cgtatcatat ccgataaaat
      721 gccccgccag ccccaaccag agtaaatgac tatgaattcg ttccagttcg ccaattaagg
      781 tgcggatata gcgcgctcgc agtggaattt cgatattatc aatgtcctcg atagcatgta
      841 cgcaggcaag cggatgcgaa gttgagcaga tgccacaaat tctttcaact agataaatag
      901 attgggtaaa agtcttggat tcgctgatga actcatgtcc gcggtgcatc cagccgattt
      961 ccatatcaat atcaaccacc gtctcaccgt ccactttcag ttcgaagtac tcggcttctt
     1021 ccaatagtgg atgaaatgga ccaagtggaa tcttgttttc ggtcataatg acctcccgaa
     1081 gcgcaacggt ctgtcgggat tttccggact attcaacgtc aggagttgtt ccatacgcgg
     1141 atgcccgaca aactcgatac cccataagtc gaatatttcg cgttcgatcc acagtgcgcc
     1201 ggtcacaata tcggtgatgg tattaacctt tggattcttc ttgtcagtta aaaccacccg
     1261 tgggcaataa tagcaaccgc tggcgtcgtg cgaaaaatga tacaggattt cgagaccgtg
     1321 ataggtttct tgagcagtag cggtcgagag ccgacagcca agcgtaacga acaaataccg
     1381 ggcaatttct ttgaggtttt cattcgacac cacaaaatag agccggcgcg gtttttcgat
     1441 tacctgcgta accttatcgc ccattaattc cataaattgc gtaaactgat ccatcaatat
     1501 ctccattttc atttcataag taagactttt tgggtggcat tatatttggt ggaattcagc
     1561 cggacgagat acatcccggt gccaaccggc tgaccggatt gattgacgcc atgccagata
     1621 acggagtagg ttccggcgga ataattgatc ttcgccgatt tccaaacctc ccgtcccatc
     1681 aggtcgtaaa cgatgatctc aaccccggct tcttccggca ggtcgaatgc aacagttgta
     1741 ttaatattga agggattagg atagatgcga tgatcggcta ttattacttc cgtttgttct
     1801 ggtaaaggtt cattaatgaa aaaatcatca cccaattcct cttttcccaa tgaagcggct
     1861 gtagaatttg gatatttgga ataaagtaat atcagacatt ctactgattt aaattgggtc
     1921 gctattcctc cttttttccc gattgataaa ttatttgtta ggtactgttg taagtaagtt
     1981 ttattcagaa gtgtatatgc ggcctgttca tccatttcgt atttttcata ataaatcaac
     2041 aattcatcaa gtaaatttga tgcctcaata aatcgttcgt atttaatata ttccgttgag
     2101 ataaaatctt ttataaatat ttcaaccagg ttacctttat tagtaagaag caatgattcc
     2161 aactcagata gaattttttc ggga
//
LOCUS       DAOOMU010000176         2151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_64149_length_2151_cov_15.343034, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000176 DAOOMU010000000
VERSION     DAOOMU010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64149_length_2151_cov_15.343034"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1920)
                     /locus_tag="PLI55_09920"
     CDS             complement(85..1920)
                     /locus_tag="PLI55_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPB01188.1"
                     /translation="MKNPDLRGNVVDVDVYGLGNLDYAENYASAYSAFFISSQGYGSF
                     FDTFAKGKYKLGIGGITEIYHQTGTLDWYIFYGPTGEKIHSEYYRLIGKPKYIPIWAC
                     GPIFWRDQNNGGKDELLNDIQKFNELQIPLTACFIDRPYSNGAHEWSKMDFSDKFAEP
                     EKWIKKINNYYGMQVMTWIGPLTFSDSDFPGLFPDSRNYIDLTNPMALAEFERRLASN
                     QYAVGIKGHKMDRADEHFPLTADWYEPVTETEARNKYVYLYAKTIDKFLQNAHGRDNF
                     NFARAAYHRSQPYLSALWGGDSRNNWYGMAGNMANAIRCGFMGFPVWGNDTGGYLGEG
                     KIDELLLYMRWLEWSVWNGMFEIKIDGAGGSGEDRPPWKYSEQLQQVFRNVCQFRMEM
                     LPYIYSCANTSYKNGVLMKPLAYMYPDDENTYTVWDEFIFGSAFLIAPVFSADNERQI
                     YLPQGNWIDYYEPNKEYSGPLTISQSIPLERIPVFIRQNSIYVSGQIYQGNSKTWAGN
                     LNGKENLIIHLYPGEINENTQFTYVDYLDGDTEKTMTLQHQEGKILFSSAAIPIPSVV
                     RVTCSEKPQKVFYGGKTATFKYDKQSKIAEIACPQNRSIQLEIRY"
BASE COUNT          623 a          482 c          402 g          644 t
ORIGIN      
        1 ataccatttt caatcgtagg acaggcattc ctgcctgtca actataggtt gacgatgaca
       61 gcctagaaag gctgtcctac gatgttaata tctaatttcg agttggattg agcgattttg
      121 cggacaggca atttcagcga ttttactttg tttatcgtat ttaaaagttg ccgttttgcc
      181 gccgtaaaat actttctgtg gcttttccga acacgtaacc ctgacaaccg atgggattgg
      241 aattgccgct gaagagaata gtattttccc ttcttgatgt tggagtgtca ttgttttttc
      301 tgtatcaccg tccagataat caacgtaagt aaattgggta ttttcgttga tttcaccggg
      361 atagagatga attattaaat tttcttttcc attcaggttg cctgcccaag tttttgaatt
      421 tccttgatag atttgtcctg aaacataaat tgaattttgc ctaataaata ccggtattcg
      481 ttctaatgga attgattgac taattgtcaa gggccctgaa tattctttat tcggttcata
      541 atagtcaatc cagttccctt gaggcaagta aatctggcgc tcattatccg ctgaaaagac
      601 cggtgcaatc agaaaagccg agccaaaaat aaattcatcc caaaccgtgt aggtattttc
      661 atcatcggga tacatataag ccaagggctt cattagtact ccgtttttat aactggtatt
      721 agcacaggaa taaatatatg ggagcatttc catccgaaat tgacaaacat tgcggaaaac
      781 ctgctgaagt tgttcagagt atttccacgg cggacgatcc tcaccgctcc caccagcgcc
      841 gtcaatttta atctcgaaca tcccgttcca aacactccac tccaaccagc gcatatatag
      901 cagcagttcg tcaattttcc cctccccaag atacccaccg gtatcatttc cccaaacggg
      961 aaaacccata aagccacatc gaattgcatt agccatattg cccgccattc cataccaatt
     1021 attacggctg tcaccacccc acaaagcact cagatatggc tgagaccgat ggtaagccgc
     1081 tctggcaaag ttaaaattgt ctcggccatg ggcattctgg agaaatttat caatcgtttt
     1141 agcatacaga taaacatatt tattgcgcgc ttcagtttcc gtaacaggtt cataccaatc
     1201 agcggttaga ggaaaatgtt catcagctcg atccatttta tgccccttga tgccaaccgc
     1261 atattggttc gatgctaagc gtcgttcgaa ttccgccaac gccatagggt ttgttaaatc
     1321 aatgtaattt ctagaatctg gaaacaagcc cggaaaatca ctgtcggaaa aagtcagtgg
     1381 cccaatccag gtcattactt gcattccata gtaattatta atctttttta tccacttctc
     1441 tggctcggca aatttatccg aaaaatccat tttcgaccac tcatgggcgc cattactata
     1501 aggccgatca ataaagcagg cggtcaaagg aatttgtagc tcattgaatt tttgtatatc
     1561 attaagcaat tcgtctttgc cgccattatt ctgatcgcgc cagaaaatcg ggccgcaagc
     1621 ccaaatcgga atatattttg gtttaccgat cagacggtaa tattcgctat ggattttttc
     1681 tccggtggga ccgtagaaaa tgtaccagtc cagcgttccg gtttgatgat aaatctcggt
     1741 gattccacca attcccaatt tgtatttccc cttggcaaag gtatcaaaaa aactgccgta
     1801 accttggcta cttatgaaaa aagccgaata agcggaagca taattttcgg cgtaatccaa
     1861 attaccaagg ccatacacat caacgtcaac gacattgcct ctcaagtcag gatttttcaa
     1921 attccccgga tacagtttct caattaagcc gaaataatgc tcattttgat cttttaagca
     1981 aactgtgata tgattaaaag tctgatgcga ggcaaaaaag tggaaagtat tttctgattg
     2041 cgtgatcttt aaattaattt cctaagggaa atccgcatgg aatccatcct tttccgccag
     2101 gcgtaatttc aaaattaatg aatcctcgaa aacctgttca actataatag t
//
LOCUS       DAOOMU010000177         2145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_64403_length_2145_cov_17.363158, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000177 DAOOMU010000000
VERSION     DAOOMU010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64403_length_2145_cov_17.363158"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..1336)
                     /gene="rho"
                     /locus_tag="PLI55_09925"
     CDS             complement(77..1336)
                     /gene="rho"
                     /locus_tag="PLI55_09925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPB01189.1"
                     /translation="MENELTIAKLQSMKIKELSDIANQLEIPETTGLKKMDLIYKILE
                     ASAQKEGKIFAQGVLQVMDEGYGFLRSEKSSYLPGSDDIYISPSQIKRFGLRTGHIVC
                     GQIRPPKDNERFFALLRVDAVNMMDPEEVKAHPVFDSLTPLYPQSRIQLETDSKNISM
                     RIMDLLCPIGKGQRGLIVAQPKTGKTTLLQQIANSITTNHPEITLMVLLIDERPEEVT
                     DMERSVKAEVISSTFDEPPERHVQVADMVLEKAKRMVELGFHVVILLDSITRLGRAHN
                     AVVPHSGKILSGGIDANALHRPKRFFGSARNIEGGGSLTIIATALIDTGSRMDDVIFE
                     EFKGTGNMELVLDRRLSDRRIFPSMDVNRSGTRKEELLLSAAELARVWILRKLLSEMT
                     PIEAMEFLIDRMNRTKSNREFLKSMNQ"
     gene            1943..>2145
                     /locus_tag="PLI55_09930"
     CDS             1943..>2145
                     /locus_tag="PLI55_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01190.1"
                     /translation="MPNFPVLFSFAEKRTKKGNGLRTSHGGSFSPSEWNLRRKSYQVH
                     ITLVIEERVMDNYESAITALKIP"
BASE COUNT          640 a          450 c          415 g          640 t
ORIGIN      
        1 atcaaataaa gcaagtgata aatatctgtt attacaatat attagatact gtttcagaaa
       61 gccctataat agccgtttac tggttcatag atttgagaaa ttcacgattg ctcttggtgc
      121 gattcatacg atcaatcagg aattccatag cttcaatcgg agtcatctcg cttaataatt
      181 tccgcaaaat ccagactcgt gccagttccg ccgctgaaag taagagttcc tctttgcgcg
      241 taccggaacg attgacatcc attgaaggga aaatacggcg atcgcttaac cggcggtcca
      301 gtactaattc catattaccg gtgcccttaa actcttcaaa aatcacgtca tccatacggg
      361 aaccggtgtc aatcagagcc gtggcgataa ttgtcaggct accgccgcct tcgatatttc
      421 gggcagaacc aaaaaagcgc ttggggcggt gtagagcatt ggcgtcaata ccaccggata
      481 ggattttacc actgtgggga acgacggcat tatgagctcg cccaagacgg gtaatactat
      541 ccagcaaaat gacaacatga aatcccagtt cgaccatacg cttggctttt tctaagacca
      601 tatctgccac ttgaacatga cgttccggtg gttcatcaaa agtcgaacta atgacctcgg
      661 ctttgacgga acgttccata tcagtgactt cctcgggacg ttcgtcaatt agcaatacca
      721 tcaacgtaat ttcagggtga ttggtagtaa tactattggc tatttgctga agcaaagtcg
      781 tcttcccggt ttttggttga gcgacaatca gtcctctctg gcctttgcca attggacaaa
      841 gcaaatccat aatgcgcatc gagatgtttt ttgaatcggt ttcgagctga attcggcttt
      901 gaggatataa aggcgtcagg gaatcaaaaa ccgggtgcgc ttttacctct tccggatcca
      961 tcatattaac cgcatcaacg cgaagaagtg cgaaaaaacg ttcgttgtct ttaggaggcc
     1021 gtatctgtcc acaaacaata tgaccggttc gcaaaccaaa tcgtttgatt tgagaaggtg
     1081 atatgtatat atcatcagaa ccaggtaaat aactcgattt ctcagagcgt aaaaatccat
     1141 agccttcatc catcacctgt agaacaccct gggcaaatat tttaccttct ttttgagcac
     1201 tggcttcaag aatcttataa atcaaatcca tttttttaag gccagttgtt tctggaatct
     1261 ccagctgatt agctatatcg gacaattctt tgattttcat cgattgcagt tttgcaatag
     1321 tcaactcatt ttccatagtt attccttttt taaaggtttt acacgaatcg ttagtctaaa
     1381 tagaggtagg tcaaaaaatt aattgattat taattagtat ctttaattta aatagtcaaa
     1441 ttcataagtc aaatataatt tttctcagca gtatattaaa tggacattag gtcagaagat
     1501 cgcgtttact cgtaatcagt aatttttcac tcttaaattc agcttttttt gctaaagcta
     1561 agattgggac taagcaagga gacttgactg tagtatcaaa aaacctgaat cattcttaaa
     1621 actttcttag caggcttgag tcgagttgaa ttttatgaat tctccggtaa atgctccctc
     1681 atcggagact cctccgaagc cactgaagaa ttcatggatt gttctaattt gtgccaaaac
     1741 tataacgttg acacgaaata ggtaaggcga ttggctaaat cgctaccttg gcactggccg
     1801 agtcgctaac tcgacccaca ttatcttttt actttacttc agcctaaaag aaagtttaag
     1861 gttgagaaaa aagactaaga tttgaattct ctctcaacct cttttctatt tactcagcct
     1921 ctttactaat gactaatgac caatgcccaa ctttcctgtt cttttttcct ttgccgaaaa
     1981 aagaaccaaa aaaggcaacg gcttaagaac atctcacggg ggttcgttca gtcccagcga
     2041 atggaattta aggcgcaaaa gttatcaagt tcatataacg ttagtgatag aggaaagggt
     2101 aatggataat tacgaaagtg ctattaccgc gctgaaaatt ccatt
//
LOCUS       DAOOMU010000178         2135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_64831_length_2135_cov_20.745192, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000178 DAOOMU010000000
VERSION     DAOOMU010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64831_length_2135_cov_20.745192"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="PLI55_09935"
     CDS             <1..822
                     /locus_tag="PLI55_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01191.1"
                     /translation="IKVAAAARKGLTSFRLMNAVANYLRAVPVEMAIVITMTDNEAMA
                     PILAGRVGMPPFHPINRLVINYIFPLFRPQVTGGYQIRSFRESDLDHLTRLFTDFFRN
                     YSLTFEWTPKRLSALLNQPNFSSENILIATKNGRPVAALTWWDQASFKRTIIEQYGGG
                     LKTLSGILKPLKILPRAGEPLNELHIRYIVYEEEHTSAARDLIRYLINEKKRKYRLFR
                     IGFQQNSPLAALLSGLPHLKIHLNCYIASDKKDDETRLLISGLRQGQIWEDLSLH"
     gene            819..1760
                     /locus_tag="PLI55_09940"
     CDS             819..1760
                     /locus_tag="PLI55_09940"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPB01192.1"
                     /translation="MKKKKSISIICNLQACGGKARRRWLDFLKIATQAGFSIDFHITE
                     YHGHATVLARERLAKGADRIIVFGGDGTLNEALNGIIVKDRPVNPDLRLVYLTAGSSC
                     DVAKMFPNPQTLLQRIADGKDYSVDVGKLECLNARAEKVVRYFLANSSIGVISRSIEK
                     FNQKRPTMNLLKRINIDLAAVSAGIKTIIQFENIDSRISYENQPFTTQKIKNITVFKS
                     PYFGGGMNYGVPTSYNDHFLHVATIAAMDAVHTFRMIPLLYSGTILKTPRAEYCRVQN
                     LSFDAPGQHIPIEADGEIIGFPPCHYTILPELIHLVV"
     gene            complement(1881..1953)
                     /locus_tag="PLI55_09945"
     tRNA            complement(1881..1953)
                     /locus_tag="PLI55_09945"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1918..1920),aa:Thr,seq:cgt)
BASE COUNT          615 a          477 c          455 g          588 t
ORIGIN      
        1 ataaaagttg ccgccgccgc tcgtaaaggc ttgaccagct tccgcttgat gaatgccgtt
       61 gccaattatt tgcgggcagt tcccgtcgaa atggctatag taatcacgat gactgacaat
      121 gaggcgatgg cacccatatt agctggtcgg gtcggtatgc cgccttttca cccaattaat
      181 cggttggtaa tcaattacat ttttccactt tttcgaccgc aagttactgg tggctaccaa
      241 attcgatcgt tccgtgaaag cgacctcgat catctgacac gattgttcac tgattttttt
      301 cgaaattact ctttgacatt cgaatggaca ccgaaacgcc tttccgcttt attgaaccag
      361 ccgaatttta gttcggaaaa catactgatt gccacaaaaa atggtcgccc ggttgcggca
      421 ttgacttggt gggatcaagc cagttttaag cgaacaatta tcgaacaata cggcggcggg
      481 cttaaaacac tatctggcat attgaaacct ttaaaaatac tgccgcgggc aggtgaaccg
      541 ttaaacgaat tgcatattag gtatatcgtt tatgaagaag aacatacctc agccgcccgc
      601 gatttaatcc gatatctaat caatgaaaaa aagcgtaagt accgcctctt tcgcatcggt
      661 tttcaacaaa attcacctct cgcggctttg ttatccggat taccgcatct gaaaattcat
      721 ctgaattgct acatcgcctc ggataaaaaa gacgacgaaa cccgcttgct aatttccgga
      781 ctgcgtcaag gtcaaatctg ggaggatctg tcattacatt gaaaaagaaa aaaagcattt
      841 ccatcatttg taacctgcaa gcctgcggtg ggaaagcccg tcggcgctgg ctggatttct
      901 tgaaaatcgc aacccaggct gggttcagta tagattttca tatcacggaa tatcacgggc
      961 atgcgacggt tttagcccgt gaacgtcttg ccaaaggagc cgaccggatc atcgttttcg
     1021 gtggcgacgg tactttgaac gaagctctca acggcataat tgtaaaagac cgtccggtga
     1081 atcccgactt gcgtttggtt taccttacgg ctggcagtag ttgcgatgtg gctaaaatgt
     1141 ttcccaaccc ccaaacactt ctacaacgga tcgccgatgg caaggattat tcggttgatg
     1201 tgggcaaact tgaatgtctg aacgcacggg ctgaaaaggt tgtgcgctat tttttggcta
     1261 attctagcat tggagtaata agtcgttcca ttgaaaaatt caaccagaaa cgcccaacga
     1321 tgaatttgct gaagagaatt aatattgatc tggcggcggt cagtgccggg attaagacca
     1381 ttatccaatt cgaaaatata gacagccgca tctcctatga aaatcagccc tttaccaccc
     1441 aaaaaatcaa aaacatcact gttttcaaat cgccctattt cggcggcggt atgaactacg
     1501 gtgtgccaac tagttataat gatcatttct tacacgttgc tacgattgcc gcaatggatg
     1561 ccgttcatac ttttcgaatg attcctttac tttactctgg aaccatcttg aaaacgccgc
     1621 gagctgagta ttgtcgggta caaaacctga gttttgatgc tcctggtcaa catattccca
     1681 ttgaagccga tggggaaatt atcggatttc cgccctgcca ttataccatt cttccagaat
     1741 taattcacct agtcgtctaa actttataac aaattgaact gatatttaaa gtctgaggta
     1801 caactgttgg cggtggtgtg gggaagacta tccaaaaata gccaactgaa aaaattttct
     1861 aactgtaaga aaatattttg agccacccaa cgggatcgaa ccgttgacct gacgattacg
     1921 aatcgtctgc tctaccagct gagctagggt ggcaaaaagc gatccaaatt tatcatattt
     1981 attcggttct tacaaaacaa gcaatatgca cgggagcggt tagttaaaga ttaagatttc
     2041 tcttagatat agtttaggta ccaacgctcg gaaaaaatta aatcaaacac aggagttgcc
     2101 taaaatcaaa tttcggtata atcacagttt attag
//
LOCUS       DAOOMU010000179         2131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_65017_length_2131_cov_15.391618, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000179 DAOOMU010000000
VERSION     DAOOMU010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65017_length_2131_cov_15.391618"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /gene="nuoB"
                     /locus_tag="PLI55_09950"
     CDS             complement(<1..380)
                     /gene="nuoB"
                     /locus_tag="PLI55_09950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020250377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HPB01193.1"
                     /translation="MGWYKRRLSKSLWVFHVSGSPCNNCDIEILDLLTPRYDVERFGI
                     KLVGSVRHADVLLVTGVFNKKVAPKIKKLYEQAPKPIFVVAVGTCVSGGCCFKDSYNI
                     VPSREAVIPIDLYIPGCPPKPEAM"
     gene            complement(382..1311)
                     /locus_tag="PLI55_09955"
     CDS             complement(382..1311)
                     /locus_tag="PLI55_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPB01194.1"
                     /translation="MTGWVFFYLLVFPGLLFSAVLGLLVGWVDRKVSARVQFRVGPPL
                     FQNFNDFFKLLGKETIIVKESLHSLFVLSPLVAFGVTTLITTMLGVALFYGIGFGGDL
                     LVIMYLLMIYSVMVILGGSATGNVYSSLGAGREIKLLLADELAFILVCLVPIIKSGYH
                     IKLEEILSAQVAEGMFIGSFSGALAFIVGLLCIQAKMTLPPFHIPEAETEIVEGPLME
                     YSGPLLGFWKLNQAMQWVAFPFLLILLFLGGFSEIGWGALWSVLKYLGIVVVMVLIKN
                     TNPRVRIDTALKFFWKYLTPVALLAVILAVLGV"
     gene            complement(1308..1811)
                     /locus_tag="PLI55_09960"
     CDS             complement(1308..1811)
                     /locus_tag="PLI55_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPB01195.1"
                     /translation="MKRLFIDIPMLYEAGDAVQKIECEYFYHRRNNGQFSLLEIAEFA
                     VYCRQCQDAFCVTVCPKEALERTDSGLIKRWNMRCVGCKSCTLACPFGTIFPEVINYV
                     TGKCDFCLNQLQDNPDYVPLCAKTAPLNSIRMEEIIQEEPSAQIFLYGEHLAIKSPSW
                     RHKEEKV"
     gene            complement(1808..>2131)
                     /locus_tag="PLI55_09965"
     CDS             complement(1808..>2131)
                     /locus_tag="PLI55_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HPB01196.1"
                     /translation="LRSVDKVPEGQSWDEEVCLVTKLLSKISVDPHNNPVVVCGPPVM
                     MKFGTFDLLKYGYPPQNIYLSMEKKMYCGIGQCRHCVMGPYFACKDGPVFTYEQIKDE
                     ENIWE"
BASE COUNT          654 a          553 c          417 g          507 t
ORIGIN      
        1 atcatggctt ccggcttggg cggacagccc ggaatgtaaa ggtcaatcgg tatgaccgct
       61 tctcgtgacg gcacaatatt gtagctatct ttgaaacaac aaccgccgga aacgcaagta
      121 cctaccgcca ctacgaaaat cggtttgggt gcttgttcat agagcttttt aatcttgggc
      181 gctacttttt tattgaacac acccgtcacc agaagcacat cggcgtgccg cactgaaccg
      241 accaatttaa taccgaaacg ttcgacgtca tagcgcggag tcagcaaatc gagaatctcg
      301 atatcgcaat tattgcaggg cgaaccgctg acgtgaaaaa cccacagtga tttcgaaaga
      361 cgacgtttat accatcccat ttcacactcc taaaaccgct aaaatgaccg ccagcaaagc
      421 cactggcgtc agatatttcc agaaaaattt tagcgctgta tcaatccgaa cgcgcggatt
      481 ggtattttta atcaaaacca tcactacgac gattccgaga tatttcaata ccgaccataa
      541 agcgccccag ccgatttcgc taaatccacc gagaaataac aaaattaata aaaatggaaa
      601 tgccacccac tgcatagcct gattcaattt ccagaaaccg agcaatggcc cggaatattc
      661 catcagagga ccttcgacaa tttcagtttc ggcttccgga atatgaaacg gcggcagagt
      721 cattttagct tgaatacata aaagcccgac aatgaatgcc agagctccgg aaaacgagcc
      781 gatgaacatt ccctccgcca cctgagcgct gagtatttcc tcaagtttta tgtggtagcc
      841 cgatttaatt attggcacca ggcaaactaa aatgaaagcc agttcgtcgg caagaagtag
      901 tttgatctcg cgccccgctc caagactgga gtaaacattg ccggtagccg atccgcccag
      961 aataaccata accgaataaa tcatcaataa gtacattatc accagcaagt caccgccaaa
     1021 gccaattcca taaaaaaggg cgacgcccag cattgtcgtg attaacgtcg ttacgccaaa
     1081 tgctaccagc ggcgacaaca caaaaagaga atgcaggctt tcctttacta ttatagtttc
     1141 tttgccgagt aatttgaaaa aatcgttgaa attctggaaa agcggcgggc caacgcgaaa
     1201 ttgcacccgc gcgctgactt tgcgatcaac ccacccgact aataatccca gaaccgcact
     1261 aaacaataat cctggaaaga ctaatagata aaaaaatacc catcccgtca tactttctcc
     1321 tccttatgtc gccagctcgg gcttttgatc gccaaatgtt caccatatag aaaaatctgg
     1381 gcagatggct cctcctgaat aatttcttcc atccgaatac tatttagcgg cgcggttttg
     1441 gcgcaaagtg gaacatagtc cggattatcc tgcagttgat tcaggcaaaa gtcgcacttg
     1501 ccggtcacgt aattaataac ctctggaaaa atcgtcccga aggggcaagc cagcgtgcag
     1561 gatttacaac cgacacaacg catattccag cgtttaatca agccggaatc ggtgcgttcg
     1621 agcgcttctt tgggacagac ggttacgcaa aaggcgtcct ggcattggcg acaatagacc
     1681 gcaaattcgg cgatttccag cagactaaac tgcccgttat tacgacgatg ataaaaatat
     1741 tcacactcga ttttttgcac cgcatcaccc gcctcatata acattggaat gtcaataaat
     1801 aaacgtttca ttcccaaata ttctcctcgt ctttaatctg ttcataagta aaaaccgggc
     1861 catctttgca ggcaaaatac ggacccatta cacaatgacg acattgaccg ataccacagt
     1921 acattttctt ttccatagat aaataaatat tctgcggtgg atagccatat ttcagcaaat
     1981 cgaacgtgcc aaatttcatc atcaccggcg gaccacagac aaccaccgga ttattatgcg
     2041 gatcaacaga aattttactc agcaatttag ttaccaaaca gacctcttcg tcccagcttt
     2101 gaccttccgg aactttatca actgaacgca g
//
LOCUS       DAOOMU010000180         2127 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_65208_length_2127_cov_9.603282, whole genome shotgun sequence.
ACCESSION   DAOOMU010000180 DAOOMU010000000
VERSION     DAOOMU010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65208_length_2127_cov_9.603282"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLI55_09970"
     CDS             complement(<1..188)
                     /locus_tag="PLI55_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018400018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01197.1"
                     /translation="MRCSLAVAFFGSFFGKGKKNRKVGHWALVIGHWSLVIGHWSLVI
                     SHWSLVIGHWSLVIGHWS"
     gene            878..1345
                     /locus_tag="PLI55_09975"
     CDS             878..1345
                     /locus_tag="PLI55_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01198.1"
                     /translation="MGTQQILMIVLSVIIVGVAVGVGITMFQNQAINSNRQAVIADLN
                     NFGAQAVAWYKLPKNMGGGGNEGFTTTPEGGGEPAITAGLLSYLGFVDEDGLPVEEYT
                     NDNGTYTVDFTEEKLTIEGVGKEKVATDTPVKATLTIDMGLAPGQQISVKIDN"
     gene            1680..>2127
                     /locus_tag="PLI55_09980"
     CDS             1680..>2127
                     /locus_tag="PLI55_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01199.1"
                     /translation="MLREYRYRCLAANGKAVQGIVSASSKTKARQMVQKVADKHHLKI
                     VAIQPRRNFIYTVKSPDGKKIKGKQAAFSKEEVSAALAKLGYQKATIQPVLFDIKMKP
                     PFNSVLMFIQMSSFLLNEQLSYDKILRMLADEETNPTLKETLRKIES"
BASE COUNT          623 a          390 c          504 g          610 t
ORIGIN      
        1 aatgaccaat gaccaatgac caatgaccaa tgaccaatga ccaatgacca atgactaatg
       61 accaatgacc aatgcccaat gactaatgac caatgaccaa tgactaatgc ccaatgacca
      121 accttcctgt tcttttttcc tttgccgaaa aaagaaccaa aaaaggcaac ggctaaagaa
      181 catctcacgg gggttcgttc agtcccagcg aatggaattt aaggcgcaaa agttatcgag
      241 ttcatatatt gttaaagaca taggcaagga tgattggtat agacgcaagt tttccgaaag
      301 cgctgaaaat tccatttttt ccgctgggcc tgcgcttccc agccggagat gttcttgatg
      361 ccgtaagtga aggtttggat cattggaatt ttggtaattg ggatttgttt aggatttgga
      421 atttgggatt tggatattga tggttttgcg aattgacggg atgaggattg cactcccgca
      481 acggcgggat cgttccggta ggaaatcccg ccggatcaat gacagcttgg gccattctcc
      541 ttttctcctt tctcctgttt cctgtctccc gtctccctgc ctgctcggcg cagtcaggca
      601 ggctttctcc tccaggcccg cgcttatcct ctcagtctta atattttcca gcactgtttt
      661 aacaaatccc ttgacattaa aaaaaactta attaaattaa tcggtaatta tgagtaattg
      721 gtagtgagca aagatagcca aaattgggaa agtcattcac ctatcacatt tattttattt
      781 ctattaagga agtgcttgac ttttaaaaaa acttaactaa attaataatg gcattgaaaa
      841 tgattaacaa cgcaaaaatt gcaaaggaga aaatgaaatg ggtactcaac aaatcttaat
      901 gatcgtcttg agtgtgatta tcgtcggagt cgccgtcggt gtcggtatca cgatgttcca
      961 aaatcaggcc atcaattcaa accgccaggc ggtaatagct gatttgaaca acttcggagc
     1021 acaagctgta gcctggtaca aattaccgaa aaatatgggc ggcggcggaa atgaaggatt
     1081 tacaactact cctgaaggag gaggagaacc cgcaataacc gctggtcttc tttcttattt
     1141 gggtttcgtt gatgaagatg gtttacctgt agaggaatac acaaatgata acggtactta
     1201 tactgttgat tttactgaag aaaaacttac aattgaagga gtaggtaaag aaaaagttgc
     1261 taccgataca cctgtaaaag caacattaac aattgatatg ggtttggcgc ctggtcagca
     1321 aattagtgta aaaatagaca actgattttt ttcttagttt tttattactc aaggtgtgag
     1381 gtatccggta tgagatgcct cacacttgtt atttttcggg taggaggttt tcggttatga
     1441 attgtttagt tcgaggccga gcgggatcgg ggggaggtgg cagggagggt ttgcgttata
     1501 atacgcggta ttgaaatggt ttttggtaat gtgtaaatta gaatttagaa tttagaattt
     1561 agaatttgga atttggaatt tggaatttag aatttgtttg gagcttggaa tttagaattt
     1621 agaatttgtg acttgtcctg cgtttgccgg actactttta cctttaaaaa gggattaaga
     1681 tgttaagaga gtatcgttat cgttgtttag cggccaacgg gaaggctgtt cagggcatcg
     1741 tctccgccag tagtaagact aaagcgcggc agatggttca aaaggtagcg gataaacatc
     1801 atcttaaaat tgtagcgatt caaccgcggc ggaattttat ctatacggtc aagagtcccg
     1861 acggtaagaa gatcaaaggc aagcaagcgg cgtttagtaa ggaagaggtg tccgccgcgc
     1921 tggcgaagtt gggttaccag aaagccacca tccagccggt gctattcgac atcaagatga
     1981 agccgccgtt caatagtgta ttgatgttca ttcagatgtc ttcctttctt ttaaacgagc
     2041 aactgtctta cgataagatt ctgcggatgt tagcggatga ggaaacgaat ccgactttga
     2101 aggaaactct acggaagatc gagagcg
//
LOCUS       DAOOMU010000181         2112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_65900_length_2112_cov_10.554205, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000181 DAOOMU010000000
VERSION     DAOOMU010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65900_length_2112_cov_10.554205"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1018..1896
                     /gene="mtgA"
                     /locus_tag="PLI55_09985"
     CDS             1018..1896
                     /gene="mtgA"
                     /locus_tag="PLI55_09985"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPB01200.1"
                     /translation="MFKKILKIILFIILIPVVIFVGLYIAAPDGSEFVHKNPETTALI
                     RQRTAEYQKAGKKVTLHWQWVPITKISPNIIHAVILAEDARFYEHIGFDVEAIKFALE
                     KNKKRKKYAVGGSTITQQLAKNLYLSTKKSIPRKVQELIIALKMERRLSKRRILELYL
                     NVIEFGPGIYGVEAACQHYFGKSAADVSIDEASRLVAIMPSPRRHSPYDGSNFTERRR
                     KWLLNWLYKTGHIDSLDYVILYKGRADNLLEVVDSTGIDQFMKEAIDDSIKSDLENVS
                     RFLPLDTKIQISDSIK"
BASE COUNT          660 a          367 c          369 g          716 t
ORIGIN      
        1 attttttccc tctgaatttt ccagaattaa agctaattag actcccaaag tcaagtaatt
       61 ttttaataag aaatcatttt gagtaatgat gtttagatag cgatcaatgg cgattggtgg
      121 aaagactcca atattgcgtg ggattcaaat ttagcagctt atattcttta ccgcctttcg
      181 cctgtccgct aatcaggtgg ggcaaataga taatatttcg attgtagctc gcgtatttcg
      241 agctttcatc cattaattag taaaataagc tggcttgcaa aattattttt cccaaaaaag
      301 cgcgcatttc tatatctatt tttctttaaa tacaggatta aaaagtttga ggttttccca
      361 aaaagaaaaa gcgaccagaa ttataattat taatccgaaa ttaatcggtt tcatatcgca
      421 tttcttacat ttaatttata tttttgaata cgaaaaataa atttagctct tcctgaccgg
      481 cagacaggtt gatatttgaa aaaagagaag taagggacag gaatgctgta gccagcctat
      541 attccagagt tattagcatc ttaaacagcc ttaacctgtc cccacagttc ccataatttg
      601 ttttgttccg atttgtcgga gcgatgtcat ttagcaaatt gaacctcgag ataattttgt
      661 tatctatgac taattcttta tctgccgatt tcagtttcat tttttttatc gcaaatcaat
      721 gtaaaaaata tcgaaaaatt gagagatcga tatttattaa tcttcatagt tagttccgtt
      781 cccttactaa aattatggtt ctatgttttt tgggaaagcg tcatttgaag gtcctgctag
      841 tagaataaat aatattgtaa tcgtgaaatc aaaaagcata tgaattctat atgctatttc
      901 ccaaataatc aatttgttat atatataatt ttctcattta aataggccaa aattgttcat
      961 aaagtctaaa ttatctttca ccttttattc ttttttctgt aaactcgtgt cagttttatg
     1021 ttcaagaaaa ttttaaagat aattctattt atcattttga ttccggtagt aattttcgtt
     1081 ggattatata ttgcggcgcc ggacggttcg gaatttgtcc ataaaaatcc ggaaaccacc
     1141 gctttgattc gccagcgtac agccgagtat cagaaagccg gtaaaaaagt tacactccat
     1201 tggcagtggg ttcctatcac taaaatctca ccgaatatta ttcatgctgt aattttagct
     1261 gaggatgctc ggttttatga acacattggt ttcgatgtgg aagccatcaa attcgcactc
     1321 gaaaaaaata agaaacgtaa gaaatacgcg gtcggcggtt cgacgatcac tcagcagtta
     1381 gcaaaaaact tatacctttc gactaaaaag tccattccgc gaaaagtcca ggagctaatt
     1441 atagcgctta aaatggaacg tcgcctatct aaaagacgta ttctcgagtt atatttaaac
     1501 gtcatcgaat ttgggcctgg aatttacggc gtggaagccg cttgccagca ttatttcggc
     1561 aaatccgcgg ctgatgtcag cattgacgaa gccagccgtt tggttgcaat catgcctagt
     1621 ccgcgtcgtc attcgcccta tgatggatcc aactttactg aacgacgacg taaatggctg
     1681 ttgaattggt tatacaaaac cggtcatatc gactcgctcg attatgttat cctctacaaa
     1741 ggcagggcag acaacctgct ggaggtggtt gattccaccg gaatagatca gtttatgaaa
     1801 gaagctattg atgattcaat caaatctgat ttagaaaatg tatcgcgatt tcttcctcta
     1861 gatacgaaaa ttcaaatcag tgattctata aaataatacc gaataacaag ttttaatgag
     1921 gcttttctat ttctaacctt ccttcagatc gaggtatgat tcttaatcaa tattttcatt
     1981 ttaataaatc agataatttt tctttgtttt tttccataat aatattaata ttgatgcaca
     2041 tttgtctggg tagacagggt tttttaaaat tattataaag gagagtaaaa tgaaaaaatt
     2101 gctggtaatt tt
//
LOCUS       DAOOMU010000182         2090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_66927_length_2090_cov_3.453563, whole genome shotgun sequence.
ACCESSION   DAOOMU010000182 DAOOMU010000000
VERSION     DAOOMU010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66927_length_2090_cov_3.453563"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLI55_09990"
     CDS             <1..317
                     /locus_tag="PLI55_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HPB01201.1"
                     /translation="ADRSEIQADGKDLSFITVRVADRNGLTVPQATNLITFKVEGCGE
                     IIATDNGDPTDFTPFPTSARKAFSGLALVIVRSRPGQSGTITVTAESPGLKKAKAFIT
                     SK"
     gene            357..>2090
                     /locus_tag="PLI55_09995"
     CDS             357..>2090
                     /locus_tag="PLI55_09995"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPB01202.1"
                     /translation="MQFYLKILICIIPLNFLLGQSPSFQTYINPVIPGDHPDATLTRI
                     GNDLYTTGSSFNVTPVIYHSTDLVHWEAIAQPVSAAWSNYGDQAGGGCWGGQVVYYNG
                     EYWHYFSRANTMYYVKASAPEGPWSMPVKVNNPSTLPYGLGYDNSIFIDDDNKWYLVV
                     KNGQPNNGIVELGDDGQPTGVVYNLNWLNPAPEYPYSWAEGPVMWKYNGYYYYSFARD
                     LGGGQKVMRSRTLTADQSAWEILGDFFNESDPQKSSSLFTSPNHASAAIMLDDSTSWV
                     IHPLYAKGEWKGQGRQGLLNQVHYDANGKPIADYPVNKSFTAPKLPSSGIPWMVPHSD
                     FFTSSALHPEWSFLGYTSAVKYSLTDRPGWLRLSPKNRTQANTVIKNDGERNYSLITR
                     LDFNAQSTSDQAGLWIMRGDQTMEVKLVSTFSTEGLKLITFSFKDTKYETGNTIGDTL
                     WLKLVRINHKISGYFSGDGFDWVEIGSPIDISEIDSYSDFSTFTGTRQGLYVRGTSDA
                     WFDFYIYRDAYTPIMAECPANQFGTALVVTKLPPNLLGKIHNDDWALYAGIEFGNAEY
                     AKSCDSLKITAS"
BASE COUNT          569 a          483 c          481 g          557 t
ORIGIN      
        1 tagccgatcg cagtgaaatt caagccgatg gaaaagattt gtcctttatt accgtgcggg
       61 tggctgatcg aaacggactt actgttccgc aggcaactaa tttaatcacc tttaaagttg
      121 aaggttgcgg cgaaattatc gccaccgata atggcgatcc gaccgatttc acgcctttcc
      181 cgacgtcggc aaggaaagct ttcagcggtt tggctctggt gattgtgcgt tccagaccag
      241 gtcaatccgg tacaataacc gtcaccgccg aatcgccggg actaaaaaaa gccaaggcat
      301 ttatcacgag caaatgattg gataatcaaa gaactaaatt aatcaagagg acttcgatgc
      361 aattttacct taaaatatta atttgtataa ttccactcaa tttccttttg gggcagtcgc
      421 cgtcatttca gacctatatc aatccggtaa tacccggcga tcatccggat gccacattga
      481 cccgcatcgg caatgattta tataccaccg gttcgtcatt taacgtaact ccggttattt
      541 atcattcaac cgatctggtg cattgggagg ccattgccca accggtgagc gccgcctggt
      601 cgaattatgg cgatcaagcc ggcggcggtt gttggggcgg tcaggtggtt tattataacg
      661 gtgaatactg gcattatttt tcacgagcca acacgatgta ttatgtaaaa gcatctgcac
      721 cggaaggccc ctggagtatg cccgtcaaag tcaataatcc atccaccctg ccgtatggtt
      781 tgggttatga taattccatt tttattgacg atgataataa atggtattta gtagtgaaaa
      841 atggacaacc caataatggc attgtcgaac tcggcgatga cggacagcca actggcgtcg
      901 tctataatct caactggcta aatcccgctc cggaatatcc ttatagttgg gcggaagggc
      961 cggtgatgtg gaaatataac ggctactatt actattcgtt cgcccgcgac cttggcggag
     1021 ggcaaaaagt tatgcgcagt cgcaccctga ctgccgatca gtccgcttgg gagatattgg
     1081 gcgacttttt caatgaaagt gatccgcaaa aaagttcctc acttttcacc agtcccaacc
     1141 atgcttcggc ggcgattatg cttgacgata gtacttcgtg ggtcatccat ccactttatg
     1201 ctaagggaga atggaaagga caggggcgac agggattgtt aaatcaagtt cattatgatg
     1261 caaacggcaa accgatagct gattatccgg tcaataaatc attcactgca ccgaaattgc
     1321 cgagtagcgg tattccttgg atggttcccc actcggattt ctttacttcg tccgctctgc
     1381 atccggaatg gtcattttta ggatatacat ccgccgttaa atattcactc accgatcgtc
     1441 ccggctggtt gcgtctatcg cccaaaaatc gtacacaagc taatacggtt atcaaaaacg
     1501 atggagaacg taattattcg cttattaccc gactagattt taatgctcaa tcaacttctg
     1561 accaggccgg tctctggatt atgagaggtg atcaaacaat ggaagtcaaa ctggtcagca
     1621 ccttttcgac ggaaggcctc aagttgataa cctttagttt caaagatact aaatacgaga
     1681 ctggaaacac catcggcgat actctttggt taaaattagt ccgcataaat cataaaatca
     1741 gcggctattt tagcggcgac ggattcgatt gggtcgaaat aggctctcca atagacattt
     1801 cggaaataga ttcctactct gatttctcaa cctttaccgg aacgcgccag gggttgtacg
     1861 tgagaggaac gagcgatgcc tggttcgatt tctatatcta tcgcgatgct tatacgccga
     1921 taatggctga atgcccggcg aatcaattcg gcaccgcttt ggtggtgact aaactcccgc
     1981 cgaatttact cggcaaaatt cacaatgatg actgggcatt atacgccgga atcgagtttg
     2041 gcaatgcgga atatgcgaaa agttgcgatt cactaaaaat aaccgcttcg
//
LOCUS       DAOOMU010000183         2075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_67597_length_2075_cov_9.103465, whole genome shotgun sequence.
ACCESSION   DAOOMU010000183 DAOOMU010000000
VERSION     DAOOMU010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67597_length_2075_cov_9.103465"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..1870
                     /gene="treF"
                     /locus_tag="PLI55_10000"
     CDS             326..1870
                     /gene="treF"
                     /locus_tag="PLI55_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HPB01203.1"
                     /translation="MKNRLLHYSLIVVLIWIPLFCGRTSKSLPEDLFEAVQMNQVFSD
                     QKKFVDCIPRIDPDSISKIYQQQKDRSDFNLRNFVGQYFDTCLVNPDTNLVLQQINFL
                     WDYLVRQPIIEHHKYSSLIELPKPYIVPGGRFREIYYWDSYFTMLGLREAGKNELIEN
                     MIDNFAYLVNSYGYVPNGNRKYYLSRSQPPFLALMIDLLNTIKNDEVYNEYLDILEKE
                     YCFWMRGAGDLGKHQNQIERVVLVEENGILNRYWDNLQTPRPESYKQDVELFLRSDRD
                     SMIFRDIRAAAESGWDFSTRWFSDGQTLETINTTQIIPVDLNCLLYHLEMTLSQAHLS
                     RNNLNESNKYKRLASRRKQLIGKYFWNEEKGYFFDYNIKTSCQMTRFTLAGLYPLFFN
                     LADTLQAEKVIKVIEDEFLKAGGLVTTVAPASGQQWDYPNGWAPLQWIGYKACQNYGF
                     YTTANEIARRWLALNAGVFFETGKMMEKYDVVNIHRAGGGGEYPLQDGFGWTNGVFLA
                     LWNELH"
BASE COUNT          601 a          405 c          421 g          648 t
ORIGIN      
        1 tggcttacca ggattgggga gtaatctatt ctcaactttc aatttatttt gaagagattt
       61 tgaaaaatta tgtctaagtt taaatgcagt ttacccaatt tgtcttatac tactcattag
      121 tatatctcct tgtttattat tgaattataa tacaataact taaggatttt caagctaatg
      181 cacatcttat tttttaggtt tttattccac acaacgggtg atgattatta aaatttcaac
      241 acaattatat tgtattgtcg attccatcca tttttatttt ttggcagtct gggttatctt
      301 acattataat taaatgaggt cattaatgaa aaacagatta ttacattatt ctttgatcgt
      361 tgttttgatc tggattccgc tattttgcgg cagaacatct aaatcacttc ctgaggatct
      421 tttcgaagcc gtgcagatga atcaggtctt cagcgatcag aaaaaatttg tggattgcat
      481 accacgtatc gatcccgata gtatcagtaa aatttaccaa cagcaaaagg atcgaagcga
      541 cttcaatttg cgcaatttcg taggtcagta tttcgatact tgtttagtaa atccggacac
      601 aaacctagtg cttcagcaaa tcaattttct ctgggattat ttagttcgac agccgataat
      661 tgaacaccat aaatattcct cactgatcga acttcccaaa ccttatatcg tgccgggtgg
      721 acgtttccgc gaaatctatt actgggatag ttacttcact atgctcggac tacgggaagc
      781 cggaaaaaac gaactgatcg aaaatatgat agacaatttc gcttatctgg taaattctta
      841 cggatatgtg cccaacggca atcgcaaata ttacctaagt cgttcccaac caccttttct
      901 agcgttgatg attgaccttt tgaacaccat taaaaatgat gaggtttaca atgaatatct
      961 ggatattctc gaaaaggaat attgcttctg gatgcgcggc gctggagact tgggaaagca
     1021 tcaaaaccaa atcgagcgag tcgtgttggt ggaggaaaat ggtatattga accgttactg
     1081 ggataatttg caaacgccgc gtccagaatc atataaacag gacgtcgaac ttttcctgcg
     1141 ttccgatcgg gattcaatga tattccgaga tattcgtgcc gccgccgaat cgggctggga
     1201 cttcagtacc cgctggttta gtgacgggca aactttggag acaatcaaca ctactcagat
     1261 tatccctgta gacctgaatt gtctattata tcatctggaa atgacactta gtcaggcgca
     1321 tctttcccgc aataatttga acgaatccaa caagtataaa cgacttgcct ctcgacgtaa
     1381 gcaattgatc ggaaaatatt tctggaatga agaaaagggt tatttttttg attataacat
     1441 caaaacatcc tgccagatga cgcgttttac tctcgccggt ctttacccac tattttttaa
     1501 tttagctgac actcttcagg ctgaaaaagt gataaaagta atcgaggatg aatttttaaa
     1561 agccggggga ttggtgacaa cggttgcacc tgcgtccgga caacaatggg actatcctaa
     1621 tgggtgggcg ccactgcaat ggatcggtta caaagcctgt caaaactacg gtttttatac
     1681 gacggctaat gagattgccc gacggtggct cgccctaaat gccggagtat ttttcgaaac
     1741 tggtaaaatg atggaaaaat atgatgtggt aaatattcac cgcgcaggcg gcggcggtga
     1801 gtaccctctt caggatggat ttggttggac caatggtgtg tttttagcac tttggaacga
     1861 gctccattga agtaaggaca acgctactaa cggacataat tccgttattc agcgtaaggt
     1921 actttcattc aattttttcc atcttagcca caaagactcc ccgatacggt cgttcctcct
     1981 tatgggttag gcgtgtcggt gcttgcaggt atttttaata atttagcctg cttaattcat
     2041 aaatccatat aaagttgttg atataatccg gtttt
//
LOCUS       DAOOMU010000184         2064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_68149_length_2064_cov_5.723743, whole genome shotgun sequence.
ACCESSION   DAOOMU010000184 DAOOMU010000000
VERSION     DAOOMU010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68149_length_2064_cov_5.723743"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..1162
                     /locus_tag="PLI55_10005"
     CDS             251..1162
                     /locus_tag="PLI55_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HPB01204.1"
                     /translation="MIKLTRISQEPILKPEPKHSWEQAAVFNAGAVYEKGLYHMIYRA
                     TNIGGHEKYGDYINNFGYAVSKDLFNWHRLSEPVMKNNVAQELRGPEDPRIVKIGDTF
                     YMTYVGFANRFPGDYRICLATSEDLVHWQRHGVLLDEENKNSSLFPEKIKGEYLLLHR
                     RHPDIWIASSLDLKNFTNHTRIMTTINDDWQCTRIGIAGPPVRHPQGWLLFYHAVDKM
                     NTYRLGVALLDYENPRRIIARHSRPVIEPELPWEINGFVPNVIFSCATVENDDKYVVI
                     YAGADTVIGAAYVKKTDVDFEKKDWLV"
     gene            1163..>2064
                     /locus_tag="PLI55_10010"
     CDS             1163..>2064
                     /locus_tag="PLI55_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01205.1"
                     /translation="MKYFKFLVIPLFLIFLRCDVLEKESPVNLTHALDLVDSIEVNGE
                     TLYYITIYSNYPDYKPVAAKSEGISCVDDVGRFMEVLESEILKSKNYSLLAIAEGMTK
                     FLLYMCRDDGLWYNFIDENGEINTSHVNSQADFGWWSTRGIRGLAAAHFIFKQFPAEK
                     DLLGQIDKCLELTLKQMDTDLAKYPLKVAGDFGSVPAWLIKSAPDMNSELLLALCKLQ
                     RSGDFELRLAIQQIAEGLIGFQYQNDGHPLNGMYFCWHNTWHAWGANQPIALLEAYKI
                     TNDSTFINSVRIWADDFVPFLIEH"
BASE COUNT          639 a          345 c          446 g          634 t
ORIGIN      
        1 agaaacagca taagagcttg aagacggtga ttgatttacg atgaaatctt ttgatataaa
       61 ctgcttggta gagatattag gtgacctgat attgtaggag tatggataag atttagtgga
      121 tgttaagttt gtcaaagatt ttgaaaatgc cgttataact aacattacca taggattagc
      181 acgaccgaag taaaatacaa caaaactaat gacttattag gtaaaaaagt taagaaagag
      241 aaagaaatta atgattaaat tgactcgcat ttcacaggaa ccgattttaa aacccgaacc
      301 gaaacatagt tgggaacaag ccgcagtttt taatgctggg gcagtctatg aaaagggact
      361 atatcatatg atatatcggg caactaatat cggagggcac gaaaaatacg gcgattatat
      421 taacaacttt gggtatgctg tcagtaaaga tttatttaac tggcatcgcc tatctgaacc
      481 ggttatgaaa aacaatgtag ctcaagaatt acgtgggccg gaagatcctc gcatcgtcaa
      541 gattggtgat acattctata tgacctatgt cggttttgct aatcgatttc ctggtgacta
      601 ccggatttgt ttagcaacaa gtgaggattt agttcattgg caacgtcatg gggtattgct
      661 cgatgaagaa aataaaaatt cttctctatt ccccgaaaaa attaaaggcg aatatctact
      721 attacatcgg cgccatcctg atatctggat tgcctcttca ctggatttaa agaatttcac
      781 aaatcatact cgaattatga ccacaattaa tgatgattgg cagtgtaccc gtattggaat
      841 tgcaggtccg ccggtaaggc atccccaagg ctggttgtta ttctatcacg ctgttgataa
      901 aatgaatacc tatcgtctcg gtgtagcctt attagattat gaaaatcccc gccggataat
      961 cgctcgtcac tcacgtccgg ttatcgaacc ggaattgccc tgggaaatca atggctttgt
     1021 tccaaatgtt atattcagtt gtgcaacagt cgaaaatgat gataagtatg tggtaattta
     1081 cgctggagct gatacggtta ttggtgcggc ttatgttaaa aaaacggacg ttgattttga
     1141 aaaaaaggac tggctggttt aagtgaaata cttcaaattt ttagttatac cccttttttt
     1201 aatcttttta aggtgtgacg ttttagagaa agaatcaccg gtaaatctga ctcacgctct
     1261 cgatttagta gattccattg aagtaaacgg tgaaacccta tattatatta cgatttattc
     1321 aaactatcct gactataaac cggtagcagc taaaagtgaa ggaataagct gcgttgacga
     1381 cgtaggacga tttatggagg tactggagag cgaaatatta aaatcgaaaa actattcttt
     1441 actcgccatt gccgaaggta tgacaaaatt tctactgtat atgtgtcgtg atgatggctt
     1501 atggtataat ttcattgatg agaatggtga aatcaatact tcccacgtta atagtcaggc
     1561 tgattttggt tggtggtcaa ctcgtggaat acgcggttta gcggcggcgc attttatttt
     1621 taagcaattt ccagcagaaa aagatttgtt aggtcaaatt gataagtgcc tcgaacttac
     1681 tttaaaacaa atggatacgg atctggcaaa atatcctctg aaagttgcag gtgattttgg
     1741 ttctgtacca gcgtggttga ttaagtcagc gcctgatatg aatagtgaac tcctcctggc
     1801 tctttgcaaa cttcagcgaa gtggtgattt tgaattacgt ctggcaatcc aacagattgc
     1861 tgaaggattg ataggatttc agtatcaaaa tgatgggcat cctctgaatg gtatgtattt
     1921 ttgctggcat aatacctggc atgcctgggg tgctaatcag ccgatcgctc ttctggaagc
     1981 ttacaaaata actaatgatt caacttttat aaatagtgtt cgaatttggg ctgacgattt
     2041 cgttcctttt ttaattgaac ataa
//
LOCUS       DAOOMU010000185         2051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_68803_length_2051_cov_7.960922, whole genome shotgun sequence.
ACCESSION   DAOOMU010000185 DAOOMU010000000
VERSION     DAOOMU010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68803_length_2051_cov_7.960922"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1064)
                     /locus_tag="PLI55_10015"
     CDS             complement(27..1064)
                     /locus_tag="PLI55_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01206.1"
                     /translation="MDITAEILNKPFGLAIPTDEEMEKYYRGERLQAFQLGWPSVKLG
                     LLPGNLTLISGYGNQGKGTFIRQVSLQMFVRYQIRSVYWAPEDSPAEYFYGDLIHTWT
                     GKSTEKHHSNYIPENEYKIAFKEVTDMVKLIDFGDEVPTVIQILEVFTHYYTRFGCRM
                     IVIDPFNNTSDMNASDRDDKVVRSVCLAAKLWLKSHQDCFVVIVLHPKAPSGVKSGED
                     PKCPTYNDLHYGSDWSKFGDDIFMWHHPSFNSNPENNDRILSPAKIKKQKISGRKVNY
                     NMIWKFEENRILDSDWQCGLPPIKTAPVKAEPKQTDLLDQRQPDASGANPGGMKPHKG
                     LEPIGKDDLPF"
     gene            complement(1048..1440)
                     /locus_tag="PLI55_10020"
     CDS             complement(1048..1440)
                     /locus_tag="PLI55_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01207.1"
                     /translation="MGKNNYIDKKSLYGEDRIRYEQSLFDTVEAKYAGKARRIIRAIQ
                     KIPANIDKSELIAAVTDYSKSTSKYIAWLGMERDEAAHVAAKSIVDRFELNYLRNEVA
                     RCHRVIDQTNSEIFTFTGLILKRNGHNG"
     gene            complement(1427..>2051)
                     /locus_tag="PLI55_10025"
     CDS             complement(1427..>2051)
                     /locus_tag="PLI55_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01208.1"
                     /translation="IIAIDKKSKALSKALSKALSKAMQKHYQKQSEYNKTNIQDTNIQ
                     GQPDKPAPRSKFIQPTKEQIEEYCLSANINIEIDEFMDHYIANGWVQSNRKPIVDWKA
                     AVRQWKRRDKIFKRNDTNDSSNFFTDAEADKIVSAINSISDELWNEKKSDFTLDAGIN
                     AIYSPNRLKYVTWDMITQCERLRRHAEDLFKKRELSNKFNPAGYGKK"
BASE COUNT          477 a          469 c          422 g          683 t
ORIGIN      
        1 tagttcctga ttcatggcaa tttgttttaa aacggtaaat catcttttcc aatcggctca
       61 agtcctttgt gcggcttcat cccgcccggg ttcgctccgc tcgcgtccgg ttgccgctgg
      121 tctaataggt cggtttgctt cggctctgct ttcaccggcg ccgttttgat cggtgggagt
      181 ccgcattgcc agtcgctgtc cagaatacga ttctcttcaa acttccaaat catattgtag
      241 ttgactttcc ggcctgatat tttctgcttt ttgattttcg caggcgatag tatccggtcg
      301 ttattttcag ggttcgagtt aaatgacggg tgatgccaca taaaaatgtc atccccaaat
      361 ttagaccaat cagacccata atgcaggtcg ttataagtcg gacatttcgg gtcttctccg
      421 gattttaccc cgctgggcgc cttcgggtgt aatacaatca caacaaaaca atcctgatgc
      481 gatttaagcc acaatttagc tgcaaggcaa acagacctaa caactttgtc gtcacggtcg
      541 gaagcgttca tgtcagacgt gttattgaac gggtcaatta cgatcatacg gcaaccgaag
      601 cgggtataat aatgcgtaaa tacttcgagt atctgaatta cggtcggaac ttcatcgcca
      661 aagtcgatca gtttcaccat gtcggttact tccttgaatg ctattttgta ttcgttttca
      721 ggaatatagt tgctgtggtg cttttcggta cttttacccg tccatgtgtg aataagatca
      781 ccatagaaat actccgctgg gctgtcttcc ggcgcccaat aaactgatct gatttgatac
      841 ctgacaaaca tctgaagcga tacctgccgg ataaatgtac ccttgccctg attaccataa
      901 cccgaaataa gcgtaaggtt accgggcaat agtcccaatt ttaccgaagg ccatccaagc
      961 tgaaacgcct gaagccgttc gcctcggtaa tacttctcca tttcctcatc tgtcgggatg
     1021 gcaagcccaa aaggtttgtt tagaatttca gccgttatgt ccattgcgtt tcagtatcaa
     1081 tccggtaaat gtaaatattt cagaatttgt ttggtcaatt actctatggc accgtgcaac
     1141 ctcattccgc aggtaattca actcgaaccg atcaactatg cttttcgctg cgacgtgcgc
     1201 cgcctcgtct cgttccattc caagccaagc gatatatttc gatgtcgatt tactgtaatc
     1261 ggtaactgca gcgataagct ctgatttgtc aatattggca ggtattttct gaatggctct
     1321 gattatcctt ctggccttac ctgcatattt cgcctcaacg gtatcaaaca ggctttgctc
     1381 gtatcgaatg cggtcttcgc cgtataacga ttttttgtcg atgtaattat tttttcccat
     1441 aaccggcggg attaaatttg ttagacaatt cgcgtttctt gaataaatcc tctgcgtggc
     1501 ggcgcagtcg ttcgcactgc gtaatcatgt cccatgtgac gtatttcaat ctattcggtg
     1561 agtagatagc gttgatgcca gcgtcaagtg tgaagtccga tttcttttcg ttccacaact
     1621 catccgaaat agagttaatg gcgcttacga ttttatcggc ctccgcatca gtgaaaaaat
     1681 tgctgctatc atttgtatca tttcttttga aaattttatc gcgtcgtttc cattgtctta
     1741 cggcagcctt ccagtcaact atcggctttc tatttgactg tacccagcca ttagcaatgt
     1801 agtgatccat aaactcatca atttctatat ttatgtttgc tgataagcaa tattcttcga
     1861 tctgttcttt tgtaggttga ataaatttag aacgcggtgc cggtttatcc ggctgtccct
     1921 gtatatttgt atcttgtata ttggtcttat tatactcgct ttgcttttga tagtgctttt
     1981 gcattgcttt tgatagtgct tttgatagtg cttttgatag tgcttttgac tttttatcaa
     2041 tggcaattat a
//
LOCUS       DAOOMU010000186         2007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_70961_length_2007_cov_13.406762, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000186 DAOOMU010000000
VERSION     DAOOMU010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70961_length_2007_cov_13.406762"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..143)
                     /locus_tag="PLI55_10030"
     tRNA            complement(70..143)
                     /locus_tag="PLI55_10030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(107..109),aa:Arg,seq:acg)
     gene            complement(215..610)
                     /locus_tag="PLI55_10035"
     CDS             complement(215..610)
                     /locus_tag="PLI55_10035"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPB01209.1"
                     /translation="MEMIIDFPKGLRVDAHLGKFTIRTDQPLSSGGEETAPTPFALFL
                     ASLGTCAGAYVLSFCRQRNLPTEKIRLVQRTERDTATGMISRIQLEIQVPEDFPEKYY
                     DALVRSANQCIVKKHLENPPHFEVFTKVV"
     gene            779..>2007
                     /locus_tag="PLI55_10040"
     CDS             779..>2007
                     /locus_tag="PLI55_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01210.1"
                     /translation="MKLLRKSFLIFITNLFVFITLNAQSLNKAVYKPRYHDPVLEQMK
                     EQREKDISRDDSLTAAIRTQQEARRKIDKKDELSLQSDLSGVKKPASLNVFKTIDHFP
                     PIAQYQSGMCWCFCGTSFLESEVMRLTGQKIKISELHTIYYEYLEKAARFIRERGDSF
                     IGEGGEVNGVFRIMKKYGAVPAEVYTGLPHGDKHDHTRLFTEIRNYLEYCKTNNYWDE
                     EIALNSIKLILNKYLGEPPQSFIYEGQEYTPVTFLNNVLKLQLDDYCDVMSTTAVPFY
                     IRAEFKVEDNWWHDSSYINIPLAEWYKFLKKAITGGYSLAIGGDVSEPGYLGFEDVAF
                     IPDFDIPFKYINQDSREFRIYNKTTTDDHGLHLVGYTRVDDRDWFLVKDSARSSRWGK
                     YEGYYFYREDYIKLKMLT"
BASE COUNT          597 a          417 c          390 g          603 t
ORIGIN      
        1 tgcagttttt tttactctaa ttaatctaaa caaaacagcc ggttgtcacc ggctgttttt
       61 attttaatgt acgcccggaa ggattcgaac cctcaacatt caggtccgta gcctgacggt
      121 ctatccagtt ggcctacggg cgcattactc aaaaagcgat ttaatttaga acagatataa
      181 ttttttgcca accatttttt tccattttat tcatttatac aaccttggta aaaacttcaa
      241 aatgaggtgg attttccaga tgttttttaa cgatacattg gttagctgaa cgcaccagcg
      301 catcataata tttttcggga aaatcctcgg gaacctgaat ctctaattgg attcggctaa
      361 tcatacctgt agccgtatca cgctccgtac gttgcaccag ccgaatcttt tccgtcggca
      421 aattgcgttg tctgcaaaaa ctaaggacat acgccccggc acacgttccc agcgaagcca
      481 aaaacaatgc aaacggagtc ggagccgttt cttcgccacc cgagctgagc ggctgatcag
      541 tccgaatggt aaacttacct aaatgagcat ctactcgtaa ccctttcggg aaatctatta
      601 tcatttccat tactgactcc tttttgttaa ttaatttatt gtttttagat aatttttcct
      661 cttcagtggt tacagttcga ttcaattttc agttgaaaac gtcttatgaa tttcttaaca
      721 tttattgaaa tgattaaaac aattttgaaa ttaataacca ctaaaacgga gtttaaaaat
      781 gaaactatta cgtaaaagtt ttctgatttt tattactaac ctttttgttt tcattacatt
      841 aaacgcgcaa agccttaata aagcggtata taaacccaga taccacgacc cggtactgga
      901 acagatgaaa gagcaacgtg agaaagatat ttctcgtgac gattcgctga ctgcagcaat
      961 tcgcacgcaa caagaagcac gccgaaaaat tgacaaaaag gatgaacttt ctctccaatc
     1021 cgacctctcc ggagtaaaaa aaccagcttc gctcaatgtc tttaaaacaa ttgaccactt
     1081 ccctcctatc gcccaatatc aatccggtat gtgttggtgc ttttgcggaa catctttttt
     1141 ggaatcggag gttatgcgtc tgaccggaca aaaaatcaaa atatcggaat tacataccat
     1201 ttactatgaa tatctggaga aagcggcgcg gttcattcgc gaaagaggcg attcattcat
     1261 tggggaaggt ggcgaagtca acggcgtttt ccgcattatg aaaaaatatg gagcggttcc
     1321 ggcggaggtc tatactggtc tccctcacgg tgacaaacac gatcacactc gccttttcac
     1381 agaaatacgc aattatttgg aatattgtaa aaccaacaat tactgggacg aagaaatcgc
     1441 tcttaatagt attaaactga ttctaaataa atatctcggc gaaccacccc agagttttat
     1501 ctacgaaggt caggaatata ctccggtaac ttttttgaat aatgttctaa aattgcaact
     1561 cgacgattat tgcgacgtaa tgtccacgac cgctgttcca ttttatatcc gagcggaatt
     1621 taaagtcgag gacaattggt ggcatgattc gtcttatatc aatattccac tggcagaatg
     1681 gtataaattt ttgaaaaaag cgattaccgg gggatatagt ctggcgattg gcggcgatgt
     1741 cagtgaaccg ggttatctgg gctttgagga tgtagctttc attccggatt tcgatattcc
     1801 gtttaaatat attaatcagg actcccgcga atttcggatt tataataaaa ctacgactga
     1861 cgatcacggt ttgcatcttg tcggatatac ccgcgttgac gatcgtgact ggtttttagt
     1921 aaaggactcg gctcgcagtt cacgttgggg aaaatatgaa ggctattatt tttatcgcga
     1981 agattacatc aaattgaaaa tgcttac
//
LOCUS       DAOOMU010000187         1995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_71577_length_1995_cov_2.804639, whole genome shotgun sequence.
ACCESSION   DAOOMU010000187 DAOOMU010000000
VERSION     DAOOMU010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71577_length_1995_cov_2.804639"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1443)
                     /locus_tag="PLI55_10045"
     CDS             complement(<1..1443)
                     /locus_tag="PLI55_10045"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB01211.1"
                     /translation="MRVLILCIILINTLLSQSISIHGLVCEKSTNEPIIGVNVFIEEQ
                     SIGCSTDENGYFTLVDLKAGKYEITFSHIQYEKHIVSIYLEERIEFLEVEMDKAEIKS
                     PEIEVYGNKYAQQYTTSHIRIDKNDIDYLPVIGEPDLFRTLESYPGVTTANDWNLGLY
                     VRGGNRDQNLILLDGMCIYSPYHLSGIFSTFDSDVIQQANMQKIICDASNGNRLSSVL
                     NINIRDGNSDVHSGYINISMLSSKMRLEGPLPFGSYMFSIRRTYADLLANAILTVANS
                     DWRFPYNFIDGTGKIVIKPWQRHRIELSSYISRDNYDVGTADQSDFPCYIWQNSAIGG
                     KHIYFISPKINLINQISYSSFKAEYLPDDTTYTEYIDNELSSIQYKSYFSYSGNSFGK
                     IRIGGEYQNIFYRLRSGGFSYQKLTIPRTEFEEISYFFDYSKKILSKIFFSIGSRYIV
                     AQKNKTGYSPFISLSYSINPSISTNIQFSRT"
     gene            complement(1507..>1995)
                     /locus_tag="PLI55_10050"
     CDS             complement(1507..>1995)
                     /locus_tag="PLI55_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01212.1"
                     /translation="VVGINERSEINNQGSQGLLAKTTGITPFQFTLPHPRVANGEVVV
                     QDIQIEDNCEPWYKGDAEVIAKQNLYDGWTTTVDMGSYDEGTYYGKNILIAWEGTNPV
                     SNPYVKIYEDDYSSDDLMLNVATASITDEGWLNGPNAKITLKVGRNVLMDFSSFWFSI
                     GD"
BASE COUNT          589 a          388 c          358 g          660 t
ORIGIN      
        1 agtccgactg aattgaatat tcgttgaaat tgaaggattg atcgaataac taagactaat
       61 gaatggggag taacccgttt tatttttttg cgcaacaata tacctgcttc ctatagaaaa
      121 gaagatttta ctaagtatct ttttgctgta atcgaagaag taggagattt cctcaaattc
      181 tgttcgtgga atggtcaatt tctgataact aaatccgcct gatctcaacc ggtaaaatat
      241 gttttgatat tcgccaccaa ttctgatttt tccgaaagaa ttgccagaat aactaaagta
      301 ggatttatat tgaatactac tcaactcatt atcgatgtat tccgtatatg tcgtatcgtc
      361 gggtagatat tctgctttga acgagctgta tgaaatctgg tttattaggt ttattttggg
      421 tgagataaag tagatatgtt ttccaccgat tgccgaattc tgccagatat agcatggaaa
      481 atccgattga tcggcggtgc cgacatcgta attgtcacga ctaatatagg atgataattc
      541 gatgcgatgt ctttgccagg gttttatcac gattttaccc gttccatcga tgaaattgta
      601 tggaaaccgc caatcggaat ttgcaactgt cagaattgcg ttggcaagca gatcagcgta
      661 ggttctgcga atactaaaca tataacttcc aaatggcagt ggaccttcaa gtctcatttt
      721 tgaactgagc atactgatat taatatagcc gctatgtaca tcactattgc catcgcgaat
      781 attaatatta agaacactgc ttaaccggtt tccattgctt gcgtcgcaga tgattttctg
      841 catgtttgcc tgctgaatga catcggaatc gaatgtgctg aaaattcccg ataggtgata
      901 gggactatag atgcacatcc catccagtaa aatcaggttt tggtcgcgat ttccacctct
      961 tacatatagc ccgagattcc agtcgtttgc ggttgttact cccggatatg attcaagcgt
     1021 ccggaataaa tcaggctcac cgattaccgg cagataatcg atgtcatttt tgtcgattct
     1081 gatatgactt gtcgtatatt gttgtgcgta tttattacca tacacttcaa tttcagggct
     1141 ttttatttcg gctttgtcca tttctacttc taaaaattca atcctttctt cgaggtagat
     1201 tgatacgata tgtttttcat attgaatatg ggaaaaagta atttcatact tgcctgcttt
     1261 taaatcaact aatgtgaaat agccgttttc atctgtcgag caaccgatgc tctgttcctc
     1321 tataaaaaca tttacgccaa tgattggttc attggtagac ttttcacaga ccaaaccatg
     1381 gatcgaaata gactgagata ataaagtatt gattaatatt atacataaga ttaaaactct
     1441 cattagacct cactaaaaag aaagccggtg ggtactagtc aataatacta atacccacct
     1501 acatttttag tcaccaatcg aaaaccaaaa tgaagaaaaa tccattaaaa cattacgacc
     1561 aactttaaga gtaatttttg cattaggacc attaagccat ccttcatcag taattgatgc
     1621 agtcgccaca ttaagcatta aatcatcaga tgagtaatca tcctcatata ttttaacata
     1681 aggattactg acaggattag taccttccca tgcaattagg atatttttcc cataataagt
     1741 gccttcatca tatgatccca tgtctacagt agttgtccag ccatcataga gattctgttt
     1801 ggcaataact tctgcgtctc ctttatacca gggttcacaa ttatcttcga tttgtatatc
     1861 ttgtactacg acttcaccat tcgcaacccg tggatgtggt aacgtgaatt ggaatggggt
     1921 tataccggtg gtttttgcta ataatccttg gctaccttga ttattaattt cacttctttc
     1981 atttatcccc accac
//
LOCUS       DAOOMU010000188         1983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_72166_length_1983_cov_11.941909, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000188 DAOOMU010000000
VERSION     DAOOMU010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72166_length_1983_cov_11.941909"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1031)
                     /locus_tag="PLI55_10055"
     CDS             complement(12..1031)
                     /locus_tag="PLI55_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HPB01213.1"
                     /translation="MKEKIHATIYDGKEFKLTYKSLREIRPNEVLVKVDVCGVCGTDI
                     KIIAGQSHATPPVILGHEFCGHIVEKGEEVSGFSIGDFVAVDPNIYCGYCQFCRRGQV
                     NLCENLQALGVDIDGGFAEYCIVQAGQCYRLPKNTDPIQASLLEPLSCVLYGFQKAAI
                     KAGERVLIVGGGMIGIIMLKLVRLSSACQIIVIEPDIERRKFCLKMGADLVFTTSEQD
                     ERKIFSHTQGGAEVVIECVGAIPAADQVFRLVKNGGRVIIFGVSPIGGKISISPYEIY
                     RRDISVIGSFLNPFTFKTAVDLLENGRIELNDLDIKTFKLADIHNAFDNQKQHKSLKT
                     VIDLR"
     gene            complement(1031..1840)
                     /locus_tag="PLI55_10060"
     CDS             complement(1031..1840)
                     /locus_tag="PLI55_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HPB01214.1"
                     /translation="MKKAIIYILLIIGGLMFAYPFLWMISATFKPEVEIGSIGLWSSN
                     FSLHNYAAVFQKIPIGRAFLNSIFVSACVTLSVILFGSIVGYALSRLNFFGRDFLMGV
                     ILFTMVIPFQITLIPMYILMVKFGWVDSYMALIVPSMISSFGILLFRQFFMDIPQDLI
                     DAAKIDGCNDLQILFRLIYPLSKPVIITVGIITFMSSWNDVLWPLIVIRLQKLMTLPQ
                     LVTIFAVGGQAEGQLGAQLAAATLLAVPIILVYAFFQRYFIESMATTGLKS"
BASE COUNT          659 a          442 c          356 g          526 t
ORIGIN      
        1 atcaaaagat ttcatcgtaa atcaatcacc gtcttcaagc tcttatgctg tttctggtta
       61 tcaaaagcgt tatggatatc agccaattta aaagttttta tatcgagatc atttagctca
      121 attcgcccat tttcgagtaa atcaacagcg gttttaaaag tgaatggatt caaaaatgaa
      181 ccgattacgc taatatcgcg ccggtaaatt tcataaggcg atatggatat tttccctccg
      241 atggggctaa cgccgaaaat tattacgcgc cctccattct ttaccaggcg aaaaacttgg
      301 tcggcggctg gaattgcacc aacacactca ataaccactt cggcaccacc ttgagtatga
      361 ctgaaaattt ttctttcatc ttgttcggat gtcgtaaaga ccagatcagc ccccattttt
      421 aaacagaatt ttcggcgttc tatatccggt tcaattacaa taatctgaca agctgatgat
      481 aagcgtacca gttttagcat gattataccg atcattccac cacctactat taaaactctc
      541 tcaccggctt tgatagcggc tttttgaaaa ccatagagaa cacaagagag cggttctaat
      601 aagctggctt gaataggatc agtatttttc ggtaaacgat agcattgccc cgcctgtaca
      661 atacaatatt ccgcaaatcc gccatcaata tcaacgccta gtgcctgaag attctcacat
      721 agattaacct gaccacgacg acagaattgg caatagccac aataaatatt tggatcaacc
      781 gctacgaaat caccaatact aaaaccactg acctcttcgc ccttttcaac tatatgaccg
      841 cagaattcat ggcctaatat aaccggcggt gtagcatgcg attgtcccgc aataatttta
      901 atatccgttc cgcaaacacc acaaacatca actttcacca agacttcatt gggacggatt
      961 tctctaaggg atttataagt aagcttaaat tctttaccgt cataaatggt ggcgtgtatt
     1021 ttctctttca tcagcttttt aatcctgtcg tagccatact ttcaataaaa taacgctgga
     1081 agaacgcata aaccaaaatg atcggtacag ctaaaagcgt tgccgcggcc aactgcgcac
     1141 caagctggcc ctcagcctgt ccacctaccg caaagatggt tactaattga ggtaacgtca
     1201 ttaatttttg taatcgaatt acaatcaatg gccagagtac gtcattccag ctgctcataa
     1261 acgtaataat cccgacggtg attataaccg gcttggagag tggataaata agccggaaaa
     1321 gaatttggag atcattacag ccgtcaattt tggcagcgtc aattaaatct tggggaatat
     1381 ccatgaaaaa ttgccggaaa agtaaaatgc cgaaagaact gatcatactc ggcactatca
     1441 aggccatata gctgtccacc cagccgaatt taaccatcag gatatacatc ggaattaacg
     1501 taatctgaaa cggaattacc atcgtaaata aaatgacacc cattagaaaa tcacgaccaa
     1561 aaaagttcaa acgtgataga gcatatccga caattgaacc gaataatatt actgataaag
     1621 tgacacatgc tgaaacaaaa atactattta aaaaggcacg accgattggg attttctgaa
     1681 aaacagccgc ataattatgc aggctgaaat tcgatgacca gaggccgatt gagccgatct
     1741 cgacttccgg cttaaaagtg gcagaaatca tccataaaaa tggataagcg aacatcaggc
     1801 cgccaataat caggagaata tagattatag cttttttcat tagtcttttc tctccacaat
     1861 ttttttctgg atcaggacaa ctatcaggat aataagcgca aagaaaaatc caagtgtcgc
     1921 cgcataaccc atatgaccga agtaaaaggc ttgattataa atataaagca tcgccgaaag
     1981 agt
//
LOCUS       DAOOMU010000189         1948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_74097_length_1948_cov_9.806656, whole genome shotgun sequence.
ACCESSION   DAOOMU010000189 DAOOMU010000000
VERSION     DAOOMU010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74097_length_1948_cov_9.806656"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..1856
                     /locus_tag="PLI55_10065"
     CDS             327..1856
                     /locus_tag="PLI55_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01215.1"
                     /translation="MKKYSAGFGLILLVSIGFAQTYHITDFIPDSLKTWQVYGTLSSS
                     LSGDDSEAERKDIGTSEYTDEDKNSTISLTPTVYYKYIWLTPNQEFSINSNIQGLYER
                     SNNRSDQNNFNLGFSIDDVTNKYIDGRFQGIIEYTRYLFCQNRLGVSVGTNFNLNENN
                     RKTTEIEKIQSSITDHWDKNNSESHHSHHSVSLYPGIVYGRIYNGNYAAKAEEILTEL
                     KKQSLLQRDLTVAEFQEFSHLIMNHVAAYHYDSRIKNIETLTDIIDYLESIGVLKELD
                     IASFVTINDIYLFSPARKIRNFGKQFYLQSGLRYSPDVYNYEDENWYRSWYADNGIFY
                     HDSLLENYRTVTSQKNRVLSHSYWYKIGFEHYQVKSWHLWYNYGAYFDHSFSLQRYTE
                     KLKTERIDITSRSFNRSRSDEVAAFAGFNYQFTSRSVLSLPVSITYSRDRTHWNSGSK
                     NIVYFTGVTMGAEFTYYLTPKWSLTSSGQIIHSYYRYSLDKQTNRSTYGTLRFSMTYY
                     W"
BASE COUNT          626 a          340 c          361 g          621 t
ORIGIN      
        1 aaacacatca atattaaata taaaaattct tttcctttgt tcttaggttt aattaatgat
       61 agccaaaaac tatactatcg tattatgaat atttcattca ccgtgagaat aagaataggt
      121 taatttcgtg ggatataatt ttcgatattc tcgtcttttg accgtaacta tagtttttga
      181 gaaatcattt cacgaatagt tcttactacg gcgccaacta ataaaatgtt agattatagt
      241 aaaagaagtt attacattgc tggcagttct ttaatagaac aaggtaattt ggattttaac
      301 aaggagttgc agttaaggag gttgaaatga aaaagtattc tgcgggattt ggattaattc
      361 ttttggtatc cattggtttt gctcagacct atcatatcac cgattttatt ccggattcac
      421 tgaaaacctg gcaggtttat gggacgctca gttcatcgct ttccggcgat gatagcgaag
      481 ctgaacgcaa agatattggt actagcgaat acacagatga ggataaaaat tcaactataa
      541 gtcttacgcc cacagtttac tataaatata tttggttgac ccctaatcag gaattttcca
      601 ttaattctaa tatccaaggg ctttatgaaa gaagtaataa tcgaagcgat caaaataatt
      661 ttaatcttgg ttttagtatt gacgatgtga caaacaagta tattgatggt agatttcaag
      721 gaataatcga atatacccgg tatctgtttt gtcaaaatcg gctaggcgtt tcagtcggga
      781 ctaattttaa tttaaatgag aataatcgca agacaacgga aatagaaaaa attcaatctt
      841 caatcactga ccattgggat aaaaataata gcgaatccca tcattctcac cattcggttt
      901 ctctgtaccc gggcattgtc tatggccgca tttacaatgg taattatgcc gccaaagccg
      961 aagaaatttt aaccgaattg aagaagcaga gtcttcttca gcgcgatttg actgttgccg
     1021 aatttcagga attctcccat ctaataatga atcatgttgc ggcttatcat tatgattcac
     1081 gaattaaaaa tatcgaaact ttaacagata ttatagacta tctcgaatca atcggcgttc
     1141 tgaaggaact cgacatagcc agttttgtga ctatcaatga catctatctt ttcagtccgg
     1201 cgcgtaaaat acgtaatttc ggtaaacaat tttatcttca aagcggactg agatattcac
     1261 ccgatgttta taattatgaa gatgaaaatt ggtatcgcag ttggtatgct gacaatggca
     1321 tcttctacca cgatagcctg ctcgaaaatt atcggacagt taccagccag aagaatcgag
     1381 ttttgtcgca ttcgtactgg tacaaaattg gattcgaaca ttatcaggta aaatcatggc
     1441 atttgtggta taactatgga gcttattttg atcactcatt ttctctacaa agatatactg
     1501 aaaagcttaa gactgaacgt attgatatta caagcaggtc atttaacagg tcgcgtagcg
     1561 atgaggttgc ggcgtttgcc gggtttaatt accaattcac tagccgatcg gtcctaagct
     1621 taccggtcag cattacttat tcaagagatc gcacacattg gaattcagga tcaaaaaata
     1681 tcgtatattt caccggagtg acaatgggag cggaatttac ctactatctg acaccaaaat
     1741 ggtcattgac ttcatcgggc caaatcattc attcttatta ccgttattct ttggataaac
     1801 aaacaaaccg aagtacttat ggtacattga gattttcgat gacctattat tggtaaatgc
     1861 tatagggcaa ggttcaatca cgacttaccc ggatttttac cttgttttta tgcctttata
     1921 attgaaaatt aagcgtaaat gacgagta
//
LOCUS       DAOOMU010000190         1878 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_78244_length_1878_cov_9.668129, whole genome shotgun sequence.
ACCESSION   DAOOMU010000190 DAOOMU010000000
VERSION     DAOOMU010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78244_length_1878_cov_9.668129"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="PLI55_10070"
     CDS             complement(<1..1293)
                     /locus_tag="PLI55_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01216.1"
                     /translation="MLKKGLVSLGLMVVSVLTVSIAGAQNLGLTEFPIAVGEESAFCI
                     GGALGSTNGLFAILGDAQSRYNITAQLISQTGISGSRISLGQATTAPFPAGLPLVAFD
                     GTNYLVVWMEEITSTNYEVYGQFIDTSGNLVETAFLIATTNAEEEDKLRAPAIAFGGN
                     TYLVTWVNNGALIGRLIDKSGNLVGTEISINSSARENAVAFDGTSYLVAWVDEIPDTD
                     RTISAQLVSQQGALVGGNFIVDDSSYPSDNPISIGFDGSRYLIVFHEEMENEEWDLYG
                     RFVTTAGTAAERIAIADETGDQQFPAIAFDGTNYLITWFDGSAGSSKGRFFNTSGTPL
                     DLEFTIFGPLENKFPLLTGVSFASDRFCAITTRVDEDFTDGDVYGVFISPSTGIDESN
                     PHPAPATFELIGNYPNPFNASTSIKYTLPTSAQVEIAIY"
BASE COUNT          573 a          385 c          346 g          574 t
ORIGIN      
        1 gtaaatggca atttcaacct gagcgctcgt aggaagagtg tatttgatag aagtggaggc
       61 gttgaacggg ttgggatagt ttccgataag ttcgaacgta gccggagcgg gatgaggatt
      121 gctttcatca attcccgtag agggtgagat aaatacccca tagacatcac catcagtgaa
      181 atcctcatca actctggtag tgattgcgca aaaacgatca gaggcgaaag agaccccggt
      241 aagtaatgga aatttatttt ctaatggacc aaatatggta aattcgaggt ctaacggagt
      301 accagaggta ttgaagaatc ttcctttaga acttccagct gatccatcga accaggttat
      361 taaatagtta gttccgtcaa aggctatcgc aggaaactgc tgatctccag tctcatccgc
      421 aatggcgatt ctctctgcag ctgtaccggc ggtagttaca aagcgcccat aaaggtccca
      481 ttcttcgttc tccatttctt catgaaaaac tatcaaatac cttgaaccgt caaagccaat
      541 agatattgga ttgtcactgg gataggaact gtcgtcaacg atgaaatttc cacctaccaa
      601 tgcgccttgt tgacttacca actgtgcgga gatagttcta tcggtgtcag gaatttcatc
      661 tacccaagct actagataac ttgtgccatc aaaggctact gcattctcac gggcactgct
      721 gttgatagat atttctgtcc ctacgagatt tcctgatttg tctatgagtc gtccaatcag
      781 agcaccattg ttaacccagg ttactagata ggtgttacca ccaaaggcta tcgccggagc
      841 ccttaatttg tcctcttcct cagcattagt cgtggctata agaaaagctg tttccaccag
      901 attaccggaa gtgtcgataa attgtccata gacctcgtaa ttggttgagg taatctcttc
      961 catccaaacc acaagataat tcgtcccgtc gaatgccact aaaggcaatc cagcaggaaa
     1021 aggagccgta gtggcttgtc ccaatgaaat tctgctacct gaaatacccg tttgagatat
     1081 caactgggcg gtgatattat atcggctttg agcgtcacca agaatagcga ataatccatt
     1141 ggtactgcct aaagcaccac cgatacaaaa agcactttct tcacctactg caatcggaaa
     1201 ctcggtgagt cctaagtttt gagcccccgc gatagagact gtcaaaactg aaacaaccat
     1261 taatcctaaa ctaactaatc ctttctttaa catatttacc tcctcttaaa actatttgca
     1321 atttaaaagt ctaaaattct tattatcaat gattttttac tttttcaatt aacaaagaaa
     1381 tttacattag aacttacatt tttaaatcaa tattctttta tcttaaaaat aatcatccag
     1441 cattccgccc atataaaaac tttgttttta atcaattgat ataagtgtga attttcccac
     1501 cccatcgcag tctgaatgac tatatgaaaa tctgattacc ttaaattcaa aggtatcaac
     1561 agtcgttatc aaatcttagg tttaagtcct gtttccacca gattttattc ttttaaaaat
     1621 tttcggaggc tttattatcg aaacgtactc ttattaattg tttttaggag ttttaatctc
     1681 accaatattt taccggtaga atttgtcgtt aaaatggcaa attttggctg aaaaaggatt
     1741 ctttgatttt agagaaagca ttttaaaggt aaaggaaacg gaatgttttt taataacatt
     1801 atcgtaagct aatccaaaac agattattaa gactcaagat ataagtgcga tttagatttt
     1861 ctgcttggcg tgttttta
//
LOCUS       DAOOMU010000191         1850 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_80069_length_1850_cov_15.761003, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000191 DAOOMU010000000
VERSION     DAOOMU010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80069_length_1850_cov_15.761003"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="PLI55_10075"
     CDS             <1..1046
                     /locus_tag="PLI55_10075"
                     /inference="COORDINATES: protein motif:HMM:NF028470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5123 domain-containing protein"
                     /protein_id="HPB01217.1"
                     /translation="LELDGNSKIKYLVSFYMPVAQTPTLIKKIQIEDCYIHDITDDVM
                     HGEKSSGIQGYVIFDSTIINNSILHRIGTLLQYKQAGSNYIAVTNSTIATVSSYGFRA
                     CGCDASEGSALPDHTPEVVIDHTTWYNIGTTDKREIILGEKGPLFRPWTITNSIFVKQ
                     CSSGGKKIFINIKDNTTSNESCMGTITNICFWKIDKIAFYMHTVRDTIRMDPQFADTN
                     NYDFTLPVGSPLLTFGTDGKPIGDPRWGTNYVALKPAVENIPAKFGLASIYPNPFNPT
                     TTISFGLAENGFTTLAIYDLRGNLVSQIVNEYLTAGEYRFAFDGSHLQSGIYLCRLTA
                     GKLTNTRKMVLLK"
     gene            1115..>1850
                     /locus_tag="PLI55_10080"
     CDS             1115..>1850
                     /locus_tag="PLI55_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01218.1"
                     /translation="MIKKYPGLILVMLIGVGSWKLLAQTIAFPGAEGSGRFAKGGRGG
                     EVYEVTNLNNSGPGSIVDAVSQSNRTVVFRVSGTIELGSVVLKPKSNITIAGQTAPGD
                     GICIKGRIKISNNNIIIRYIRVRVDAGAANSSGDAIDIDKGKNIIIDHVSASYARDET
                     ISCQEASDSVTVQWCLMSEALTFEGHSYGSLVRGDFGDQKSYHHNLYAHCNGRLPRPG
                     NYTSLATDSLGLFFDFRNNVVYNWAGS"
BASE COUNT          507 a          401 c          436 g          506 t
ORIGIN      
        1 gtttggaatt agatggtaat tccaaaataa agtatctggt ttcattttat atgcccgtag
       61 cgcaaactcc gaccttaatt aaaaaaattc agatcgaaga ctgctacatc cacgatatta
      121 ccgacgacgt tatgcacggc gagaaaagta gcggtattca aggctatgta attttcgatt
      181 cgaccattat caataattca attcttcatc ggatcggtac ccttcttcaa tataagcagg
      241 cgggatcaaa ctatattgca gtaactaatt ccaccatcgc tacggtttca tcctatggct
      301 ttcgggcttg tggatgtgat gctagcgaag gatcggcctt accagatcac acccccgaag
      361 tcgtgattga tcatactact tggtataata tagggacaac tgataagcgc gaaatcattc
      421 tgggtgaaaa gggaccactt tttagaccgt ggactataac caattctatt tttgtaaaac
      481 agtgttccag tggagggaaa aaaatattta ttaacattaa agataacact actagcaatg
      541 aatcctgcat gggaacgatt acaaatatct gtttctggaa aattgacaag attgcttttt
      601 atatgcatac cgtcagagat acgattcgga tggatcccca atttgcggat acgaataatt
      661 atgattttac cctgccggtt ggttcaccgc tactgacatt tggcacggac ggtaagccga
      721 tcggtgatcc gcgctggggc acgaattatg tcgcactaaa accggcggtc gagaatattc
      781 ccgcgaagtt cgggctggca tcaatttacc ccaatccatt caatccgacg acgactatat
      841 cgttcggatt agctgaaaat ggcttcacga cgctggcgat ttacgacctg cgcggtaatt
      901 tagtcagtca aatagtgaat gaatatttga cagctggcga atatcgtttt gcttttgatg
      961 gcagtcatct gcaatcgggt atttatctct gtcgcctgac tgccggcaag ttgactaata
     1021 cccgaaaaat ggttctcctg aagtaaccaa tggtaattgt taaaattgtt tggggcattg
     1081 acaaccggct aagttttgta attcagaggc ttaaatgata aaaaaatatc cgggattaat
     1141 cctggttatg ttaatcggcg tcggtagctg gaagctactg gcacagacaa tcgccttccc
     1201 cggtgcggaa gggagtgggc gctttgcgaa aggtgggcgc ggtggtgagg tttatgaggt
     1261 aaccaatcta aacaacagcg gacccggatc aatcgttgac gccgtcagcc aatccaaccg
     1321 cacggtcgtt tttcgagttt ccggaacaat cgagctaggg agtgtcgttc tcaagcctaa
     1381 gtccaatata acgatagctg gtcagaccgc gccgggcgac ggcatttgca tcaaaggacg
     1441 aatcaaaatc agcaataata atataatcat tcgttatatc cgggtgcggg tggacgccgg
     1501 tgcggctaat tcttcgggcg acgccattga cattgataaa ggtaaaaata tcatcattga
     1561 ccacgtcagc gcctcctatg cccgcgatga aacgatctcc tgccaggaag cttccgatag
     1621 tgttactgtg caatggtgtc tgatgagcga agccctgact ttcgaaggac acagttatgg
     1681 atcactcgtg cgcggcgatt ttggtgatca gaagagctac catcacaact tatatgccca
     1741 ttgtaacggg cgtttgccgc gaccgggcaa ttatacttct ctggctaccg attcgctcgg
     1801 tttatttttc gatttccgca ataatgtggt ttacaattgg gctggttcat
//
LOCUS       DAOOMU010000192         1844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_80463_length_1844_cov_14.233091, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000192 DAOOMU010000000
VERSION     DAOOMU010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80463_length_1844_cov_14.233091"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1572)
                     /locus_tag="PLI55_10085"
     CDS             complement(22..1572)
                     /locus_tag="PLI55_10085"
                     /inference="COORDINATES: protein motif:HMM:NF025418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HPB01219.1"
                     /translation="MIKKRILILSLFVMTLVTMALPQYYISPYEKVYDDLLYLQAGGF
                     LRDLDLNHTPISTIQIAQSLKLQLKSDNYTTAGATQRLLIDRIAVTYAPADTDLVLNL
                     KRKLSDLLKIESGQSYLHYGVKINLDYQNQPQSKLYPQLRTFGSLSVTKNLSITNVMT
                     LDPFATENPDYVGKEWRGLSGYTEQAYLMWSSRAFRLTAGRSYIVFGPGRKSSLLFSS
                     AARPLDNLKLDIFLKHLSFQGITAKLDKMDDNERYLSAHRLSFYLGNWAIGLTEAMLY
                     TGINQNLEFAYLNPFLLYHGEQLNGPDLNGNTLGTLELSYTGRNWIAYSEFLVDDIQF
                     DKEEPGDLEPNEVGCILGMDIADPFKIPGVYLGMEYTAITNRTYKTPTRSEWFLHRNV
                     PIGYELGSDLDRFNVLVRKYHKNLQIALEFDHIRQGEGDLNKPWDSPWDSLTIEQGYS
                     EKFPTGVVQTTSDVGLEVTWMPDYGKYIYARLTYEAIKNIDHGNKNAQNLIFSVGAYL
                     DLHGKIGF"
BASE COUNT          557 a          381 c          394 g          512 t
ORIGIN      
        1 tttcgtgtgt ttcgcgggaa attaaaaacc aatcttacca tgtaaatcca agtaggctcc
       61 aacggaaaaa atcaaattct gcgcgttttt attaccatga tctatatttt tgatcgcctc
      121 gtaggtcaga cgtgcgtaaa tgtatttacc gtaatccggc atccaggtaa cctccagtcc
      181 tacgtcactg gtcgtttgaa ctactccggt tggaaatttc tccgagtaac cttgttcgat
      241 agtcagggag tcccaaggcg aatcccaggg tttattcaaa tcaccttcac cctgccgaat
      301 atggtcgaac tctaatgcaa tttgcaagtt cttatgatac ttgcgaacga gcacattaaa
      361 acggtctaag tcactgccca gctcgtaacc aatcggcaca ttgcggtgta aaaaccactc
      421 gcttcgtgtt ggtgttttgt aggtgcgatt ggtaatggcg gtgtactcca ttccgagata
      481 aacacctggg attttaaacg gatcagcaat atccatcccc agaatacaac cgacttcatt
      541 cggttcaaga tcaccgggtt cttctttatc gaattgaatg tcatcaacta aaaattcact
      601 gtaagcaatc cagttccgac cggtataact gagttccagg gtcccgaggg tattgccatt
      661 taggtcagga ccatttaatt gttcaccgtg ataaagtaga aaaggattca gataagcgaa
      721 ttcaaggttc tggttaatac cggtatatag catcgcttcc gtcaagccga tcgcccaatt
      781 tcccagatag aaacttaggc ggtgcgctga aaggtagcgc tcgttatcat ccatcttatc
      841 aagtttagca gtgattccct gaaaactgag atgtttcagg aaaatatcca attttaaatt
      901 atctaatggg cgcgccgccg aagaaaatag taatgagctc ttgcgtcccg gtccgaaaac
      961 tatataacta cgcccggcgg tcagacggaa tgctctggaa ctccacatca aataagcttg
     1021 ctccgtataa ccggatagac cgcgccattc ctttccgaca tagtccggat tctcggtcgc
     1081 aaagggatca agtgtcataa cattggtaat actgagattt ttggttacgg aaagagaacc
     1141 aaaagtccgc agctggggat aaagtttgga ctgaggttga ttctgatagt cgaggttaat
     1201 tttaacccca tagtgtaaat atgattgacc gctttcaatc tttaataagt cagagagctt
     1261 gcgctttaaa ttcaaaacaa ggtcagtatc agcgggagca taagtcaccg caatcctatc
     1321 aatcaacaaa cgttgagtcg ctccggctgt ggtataattg tctgatttca actgaagttt
     1381 taaggattgc gcaatttgaa tggtcgaaat cggagtatga ttcaaatcga gatctcgtaa
     1441 gaacccaccg gcttgtaaat agagtagatc atcataaacc ttctcataag gcgaaatata
     1501 atattgtggc aatgccatag tcactaaagt cattacaaat agcgataaga ttaaaattct
     1561 tttttttatc atcttttttc tgccttccat caaatttatc ggaaacacga catatattaa
     1621 cgcctgaaag cgaaagacca aagaggaaaa tcaagcgctg tttcgcaagc tgaatttatt
     1681 aaatacgaaa gacaccaact gcacggaatt tagtatgaat gtatgcaaat ggtcattggg
     1741 cactggtcat tggtcattgg tcatttgtaa agaggctgag aaaaaagatt gagaaaatag
     1801 atagagaagg aagacgggag acagtttcgc ggaaattggt ttgg
//
LOCUS       DAOOMU010000193         1811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_82752_length_1811_cov_29.338838, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000193 DAOOMU010000000
VERSION     DAOOMU010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82752_length_1811_cov_29.338838"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1811
                     /gene="dnaG"
                     /locus_tag="PLI55_10090"
     CDS             <1..1811
                     /gene="dnaG"
                     /locus_tag="PLI55_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPB01220.1"
                     /translation="VMPRISSNTIEQIKARTDILEIVSDFVQLKQRGRNYFGLCPFHE
                     EKTPSFSVNPSLGIFHCFGCGKGGNAVTFIMEYEKIDYVEALKRLAERYSITIDWEGI
                     EDSQKGEVALIYELHEIAAEFYQKQLMTERGRSALEYIEKRGFDRETIEQFRLGYAPD
                     EWESLFRQIDLNRFTPAILEKSGLFVRREGNKIYDRFRNRLMFPIINLAGRVVAFGGR
                     ALDPNEEAKYLNSPETPIYFKSGIFYGLNFSKDAIQKAGDAIIVEGYTDFLRVYTGGF
                     PNVVAGSGTALTLHHARLIHRFTKKVTLCYDGDEAGQKATERAGFLLMREGLDVRVIR
                     LPESDDPDSYLRDHKPEAFRELYDSANDFMTYYIDSHSDELNSPAAKTQFVERIAEEL
                     IEIKNPVILDLVVGEVAERINVKEEHIRAQIRYIARRRTNQNRGERSTESPKSLRLRT
                     ADEKAEYELLKMILTRHQNLQTMIKNFLTAADFRHPIMHPIAEKLLSDLSSGKLAEAD
                     ELFNTEWNEEQRLYLSRLILEADPLQYVENMDDLRKLTSDCIAVLVTRNIDAEIAAVR
                     EKIKAAEKKGEDSTSLVIQLSNLRQKKRALEDKIKA"
BASE COUNT          514 a          414 c          433 g          450 t
ORIGIN      
        1 gcgttatgcc gagaatttcc tccaatacta tcgagcagat caaagcgcgt acggatatac
       61 tcgagatcgt ttcagatttt gtgcaattga aacagcgggg gcgcaattac ttcggactgt
      121 gtccttttca tgaagaaaaa actccgtcat tcagtgtcaa tcccagcctg ggaattttcc
      181 actgtttcgg ttgcggtaag ggcggcaacg cggtgacttt tataatggaa tacgaaaaaa
      241 ttgactatgt cgaggctttg aaaagattag ccgagcgcta ttctatcacc attgattggg
      301 aaggcatcga agattcgcag aagggcgagg tcgccttaat ttatgaatta cacgagattg
      361 ccgcagaatt ttatcagaaa caattaatga cagaacgtgg tcgttcggca ttggaatata
      421 tcgagaaacg cggtttcgat cgcgaaacaa tcgaacagtt tcggctcggc tatgcgccgg
      481 atgaatggga gagtcttttc cggcagattg atttgaaccg ttttacaccg gcgattctcg
      541 aaaaatccgg tctttttgtt cgcagagaag gaaacaaaat atacgaccgc ttccgtaacc
      601 ggcttatgtt cccgataata aacttagccg gacgagtcgt tgcctttggc gggcgcgcgc
      661 ttgatccaaa tgaggaagcc aagtatttga attcgccgga aacgccgatt tatttcaaga
      721 gcggcatttt ttacggattg aatttctcca aggacgctat tcagaaagcc ggtgacgcga
      781 ttatcgttga aggctacacc gattttttac gagtttatac cggtggcttt cccaacgttg
      841 tagccggttc cggaactgca ttgactttac atcacgctcg tttaatccac cgtttcacaa
      901 aaaaagtgac actctgttac gacggcgatg aagccggcca aaaagccacc gaacgcgccg
      961 gatttttgct aatgcgcgaa ggattagacg tccgtgttat tcgtctgccg gaaagcgacg
     1021 acccggattc ttacttacgc gatcataaac ccgaagcatt tcgcgagctt tatgattcgg
     1081 ctaacgattt tatgacttat tatattgata gccatagcga tgaactcaat tctcccgccg
     1141 ccaagactca gttcgtggaa cgcattgccg aagagctgat cgaaattaag aatccggtca
     1201 ttctggatct ggttgtcggc gaagtcgcgg agcggattaa tgtcaaagaa gaacatatcc
     1261 gggctcagat tcgctatatc gcccggcgac gcaccaatca aaatcggggc gagaggagca
     1321 ctgaaagccc gaaaagtttg cgtctgcgca ccgctgatga aaaagccgaa tacgaacttc
     1381 tgaaaatgat tttgacccga catcaaaatt tgcaaacaat gattaaaaat tttctcacgg
     1441 ccgccgattt ccgccacccc ataatgcatc caatcgctga aaaattactg agcgacctga
     1501 gctcgggaaa acttgccgaa gccgatgaac ttttcaatac cgaatggaat gaagagcaac
     1561 gcctttattt atcacgtttg attctggagg ctgatccgct ccaatatgta gagaatatgg
     1621 atgatctccg caaactaacc agtgattgta tcgcagtttt agtgacgcgg aatattgacg
     1681 ctgaaatcgc cgccgtgcgg gagaaaatca aagccgctga gaaaaaaggt gaagacagca
     1741 cttcactggt gattcaactc tcaaacctac gccagaaaaa gcgcgccctc gaggacaaga
     1801 ttaaagcctg a
//
LOCUS       DAOOMU010000194         1810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_82825_length_1810_cov_22.188034, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000194 DAOOMU010000000
VERSION     DAOOMU010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82825_length_1810_cov_22.188034"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..1570
                     /locus_tag="PLI55_10095"
     CDS             278..1570
                     /locus_tag="PLI55_10095"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HPB01221.1"
                     /translation="MHAYSFQLYSLALLATIIIATVTAIVLWPRRKFKGVLWMILLEI
                     AAAEWAFGILFESAATTHRLKELWSAIAYIGTCSVPLFFFLFTVEYYRGRLQISKIAL
                     TFLITIPIFIIILAFTNPLHNLLWPNITINPATNIAIYSHGIVWWITYVYEYALIIIG
                     MYFLLKMAFESGSFYTPHNLTIIIGALIPIIGNLIYVFGPNPVPGLDWAPVGFALSGI
                     VITWAILRLQLFKLRPIAYSFLVDKMLDGMIILDTNNVIVDINPASANIFHKRPKQMI
                     GQKINRFLPQSIDLKNLLLAENHSQIEIALGKIQPDRIFELRLSILRDNYSQIIGKSL
                     LLRDITEKKKNENEREKLIRDLQNAMSQIKTLSGLLPICAHCKKIRDDKGYWHAVEEY
                     IHEHSEADFSHSICPDCLEKYYPEIAKKMKSSQGKISS"
BASE COUNT          570 a          360 c          325 g          555 t
ORIGIN      
        1 caggcggttt ttcgaaaaca aataatcaag attgatgacc agacaaatag agttcttcag
       61 atatgaaata ttccaggata aattttggaa tagtggcatc tgccaatatc gggagaaaaa
      121 acgctacttg cctcactgaa aacatcttta cattctgcat ctaaatgtaa atgttagctc
      181 ttgattatac gcaaattaat gaacttagat ctcaattccc tttgtaatat gggatagatt
      241 atttaggttt atctgatatt tttttggagc tattaatatg catgcttatt cctttcaatt
      301 atatagtctt gccttgttgg caaccattat cattgcgaca gttactgcta tagtactctg
      361 gccgcgtcgc aaattcaaag gcgtactatg gatgatctta cttgaaatcg ccgcggcgga
      421 gtgggcattc ggtattttat tcgaatcagc cgccactact caccgattga aagaattatg
      481 gtcggcaatt gcctatattg gtacttgttc tgttccactc ttcttttttc tcttcacggt
      541 tgaatattac cgaggacgat tacagatttc taaaatcgca ctaacgtttc tcataaccat
      601 tccaattttc atcattattt tggcattcac taatcctttg cacaatttgc tctggcccaa
      661 tattaccatc aatccggcaa ctaatatagc gatctattct catggaatcg tgtggtggat
      721 tacttacgtt tatgaatacg ctcttatcat tattggaatg tatttcctac taaagatggc
      781 gtttgaatcc ggctctttct atacgcctca taatctgacg ataattatag gtgctttaat
      841 accaataatc ggcaacctga tatatgtttt cggtccgaat cctgtgccag gattagattg
      901 ggctccagtt ggattcgctt tatcgggaat tgtaatcacc tgggcaattc tgagattaca
      961 attatttaaa cttagaccaa tcgcttattc atttttagta gataaaatgc tcgatggaat
     1021 gattattctg gacactaata atgtaatcgt agatataaat ccagcgtctg ccaatatctt
     1081 tcataaacga cccaagcaaa tgattggcca aaaaataaat cgttttctcc ctcaatcaat
     1141 tgatctaaag aatttgttac ttgccgaaaa tcatagccag atcgaaatag ccctgggtaa
     1201 aatccaaccg gatcgcatat ttgaactacg gctctctatt ttgcgagata actattccca
     1261 gataattggt aaaagtctcc ttttacgcga catcaccgaa aaaaagaaaa acgaaaatga
     1321 gcgtgaaaaa ttaatccgtg acctacaaaa tgctatgagc caaattaaaa ctctcagcgg
     1381 tcttttgcca atctgtgcgc attgtaaaaa aatacgcgac gataaaggtt attggcacgc
     1441 cgttgaggaa tacattcatg aacattccga ggctgatttt tcccatagta tttgtccgga
     1501 ttgtctcgaa aaatattacc ctgaaattgc gaaaaaaatg aaatcaagcc agggtaaaat
     1561 atccagctaa cgtctcatta aattacctta aaaattaatt atcaatacag ctaataattg
     1621 aaaggataac gaaaatgaaa gccgaaggga aaaattcaga acaagatcaa tatcggtctc
     1681 gaatattaaa aacggccgat ctgggtaatt atcagaccgg agcaatcgtt agccggacct
     1741 tgattgacag accggctgga aacgtgactt tcttcgcctt cgacgaaggt cagcgactga
     1801 gcgaacacac
//
LOCUS       DAOOMU010000195         1806 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_83131_length_1806_cov_15.143918, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000195 DAOOMU010000000
VERSION     DAOOMU010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83131_length_1806_cov_15.143918"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1408)
                     /locus_tag="PLI55_10100"
     CDS             complement(188..1408)
                     /locus_tag="PLI55_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPB01222.1"
                     /translation="MKINIYRHSGNIKRLLLVLAIVMIFALLNYTQKIVTRLRQDSAN
                     LIRFYADIYAKAAMDMSGDDFSFIFEEIIQKTSTPMILSDAEAMEPTAWRNIGVPDND
                     RSPATLAEVKKIMQEMDRENQPIPLKYEDRVLQYIHFGDTNMIRQLRRLPYIEIAIVG
                     LFILLGYVGFSLIRENEKRSIWVGMAKETAHQLGTPLTALMGWLELLKDEIGDNANIS
                     EIARDIQRLEKVANRFSQIGSRPVLKPTNINLVINDAVSYYRRRLPQLGPGISLEFIP
                     SGDYIVNLNADLFSWALENLIKNAIDAVSQENGKIRVELHLLHGGKKIAVDVIDNGKG
                     ISKSNRKNIFRPGYSTKQRGWGLGLSLAMRIVKEYHRGQLFVLMSKPNEKTVMRIVLK
                     NPQENQKREQESAF"
     gene            complement(1451..>1806)
                     /locus_tag="PLI55_10105"
     CDS             complement(1451..>1806)
                     /locus_tag="PLI55_10105"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPB01223.1"
                     /translation="QVTDYTPNRVVLNVETERPAFLVLADAYYPKGWTARVAGVETPI
                     YQVNHVLRGVPVPAGNYAVEFKFLPRSYQIASRTSSISCDIVWLSLLGVLIYQNREKI
                     KNLKKKRPTPAKSNR"
BASE COUNT          487 a          421 c          383 g          515 t
ORIGIN      
        1 atacgggcga atatttacgg atgttttgga aaaaatgcaa gtatttttta taatttacag
       61 actgacctag atacacaaca tccacttctg gtgattacat ttaactttgt tgtagtttca
      121 gcgaatcgcc aggataagtt caaattaccc ataataaaaa ggtattcaaa ggcgtgtttc
      181 actattttta aaaagccgat tcttgctctc ttttctggtt ttcctgcgga tttttcagca
      241 cgattctcat aacggttttt tcgttgggct tgctcattaa gacgaataac tgtccgcggt
      301 gatattcctt cactatccgc attgctaaac tcagacccaa gccccagccg cgttgcttag
      361 tgctgtagcc tggacggaaa atattcttgc ggttgctctt actgattccc ttgccgttat
      421 caatgacatc aacggcaatc ttcttcccgc catgaaggag gtgtaattct actcttattt
      481 tgccgttctc ctgcgatacg gcgtcaatag cgttcttgat caaattttcc agcgcccagg
      541 aaaataaatc cgcattcagg ttgacgatgt aatcgccaga tgggataaat tccagactga
      601 tgcccggtcc cagttgtggt aaacgacgcc gataatacga aacggcatcg ttaatgacca
      661 aattaatgtt agttggtttc agaaccgggc gactgccgat ctgcgaaaaa cgatttgcca
      721 ctttttctaa ccgctgaatg tcgcgcgcaa tttcgctgat attggcattg tcgccgattt
      781 catccttcag caattccaac caacccatca gcgcggtcag cggcgtgcct aattgatgcg
      841 cggtttcttt tgccatacca acccagatcg agcgcttctc attttctcgg attaacgaaa
      901 aaccgacata tcccagcaga ataaaaagcc cgacgatggc aatttcgata tagggcaagc
      961 gccgcagttg gcgaatcata ttggtatcac cgaaatgaat atattgcaat actcgatctt
     1021 cgtatttcag tggaatcggc tgattttccc gatccatttc ctgcattatc ttcttgactt
     1081 cagccagagt agcgggcgaa cgatcgttgt ccggaacgcc aatgttgcgc caggcggttg
     1141 gttccatagc ctcggcatcc gagagaatca tcggagtcga ggttttctgg attatttctt
     1201 caaaaataaa actaaagtcg tcgccactca tatccatagc ggctttggca taaatatcag
     1261 cataaaaccg aatcaaattg gcggaatcct gacgcaggcg cgtgacgatt ttctgggtat
     1321 agttcagcaa ggcgaaaatc ataacaattg ccagaacaag cagcaagcgc ttgatattgc
     1381 cagaatgacg ataaatattt attttcatgg aattacgccg ataataatca ttttagttgc
     1441 attggtaaaa tcatcggttg cttttggcag gtgttgggcg ctttttctta aggtttttta
     1501 tcttctcccg attttgataa atcaacactc caagcaggct aagccagacg atgtcgcaag
     1561 aaatagaaga agtccgggac gctatctgat aactacgcgg taaaaactta aattccaccg
     1621 cataatttcc cgctggaact ggcactcctc gcaaaacatg attgacttga taaatcggtg
     1681 tttcgacacc tgccacacga gccgtccaac ctttcggata ataagcatct gccagaacca
     1741 agaaggccgg gcgctcggtt tcgacgttta gcaccacgcg attgggagta taatcggtta
     1801 cttgaa
//
LOCUS       DAOOMU010000196         1790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_84265_length_1790_cov_16.398847, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000196 DAOOMU010000000
VERSION     DAOOMU010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84265_length_1790_cov_16.398847"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLI55_10110"
     CDS             complement(<1..369)
                     /locus_tag="PLI55_10110"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HPB01224.1"
                     /translation="MNYKSVMPIWIAFLLLVSVFIANLNSANAPDIHTWEMQEIVLQA
                     EGKYENFYTDVTCWIDLTGPEFSKRIYGFWDGANIFKVRFVATKPGKWYWRSGSNQPN
                     DNGLNNLSGEFMAVEWTETEK"
     gene            complement(445..>1790)
                     /locus_tag="PLI55_10115"
     CDS             complement(445..>1790)
                     /locus_tag="PLI55_10115"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPB01225.1"
                     /translation="VFDIRQGVAIALFVKNKKPKESSVFHADLYGLREEKYQWLQKND
                     FQKNNYEKIKPTSPYYFFKKIDTGKVKQYLKWKQITDIFPVNSVGIVTARDHFVISFD
                     KRDLKNRILQLQNLAHPDEIILQAFNLKEKFGWKIKDSRQEVANISDLDIYIKEILYR
                     PFDKRYLFYHQSLIERMRFEVMQHMLEENIGLVTIRRSRNPEKWNFAFVANKLIAGAT
                     AITSLDINYLFPLYLYPEQSKKDNPGLNLTTMLIFEPGEKYGRKPNIAPIIFSRLAEH
                     YPKRPSPEEIFYYVYAILYSNIYRETYVEFLKIDFPRVPFTADYEVFKNLGKSGKQLA
                     DLHLLRSPALDPPIARYQGSSPNDRIEKVIYQKDEQRIYINNDKYFEGIAPEVWNYYI
                     GGYQVLHKYLKDRKGRTMDDAPHYCRIVTALSKTIEIQKQIDAIYPDVEKEIIKF"
BASE COUNT          508 a          373 c          320 g          589 t
ORIGIN      
        1 tttttcggtt tccgtccatt cgaccgccat aaactcgccg ctgaggttgt ttagtccatt
       61 gtcattcggc tggtttgaac cactgcgcca gtaccatttt cccggcttag tagcgacgaa
      121 acgcactttg aaaatattag cgccatccca gaaaccgtaa atccgcttgg aaaattccgg
      181 tccagtcaga tcgatccagc aagttacatc ggtatagaaa ttttcatatt ttccttcagc
      241 ctgcaagaca atttcttgca tttcccatgt gtgaatatcc ggcgcattag ctgaattcag
      301 attggcaatg aaaacactta ctaaaagcag aaaggcaatc caaatcggca tcacactttt
      361 gtaattcatc taaacttagt atcctttcat aacgaaatga aaaatttgaa taatcgcacg
      421 actaatttcg ataaaattca ttgtttaaaa ctttatgatt tccttttcta cgtcgggata
      481 gattgcgtct atttgcttct ggatttcaat ggttttactc aaggcggtta ctatgcgaca
      541 gtaatggggc gcatcgtcca tagttcttcc cttacggtct tttaaatatt tatgtaatac
      601 ctgataaccg ccgatgtaat aattccagac ttccggtgca atgccttcaa aatatttgtc
      661 attattaatg taaatgcgct gttcatcttt ctggtaaata accttctcga ttcggtcgtt
      721 tgggctactg ccctggtatc gggcaatcgg cggatcgagc gccgggcttc taagcaaatg
      781 caggtcagcc agttgttttc ccgatttgcc tagattttta aatacttcat aatcagcggt
      841 aaaaggcacg cgcgggaagt caattttcaa gaactcgaca taggtttcgc ggtaaatatt
      901 gctgtataag attgcgtaaa cataatagaa aatctcttcc ggcgacggtc ttttgggata
      961 atgctccgcc aatcgtgaaa aaattatggg cgcaatatta ggttttcggc cgtatttttc
     1021 gcccggctca aaaatcagca tcgtagtcag attcagaccg ggattgtctt ttttcgattg
     1081 ctccggataa agatataaag gaaataggta atttatgtcc aaagatgtaa tggcagtcgc
     1141 accagcaatc aatttattgg caacaaaagc aaaattccat ttttccggat ttcttgacct
     1201 tcgaatagtt accaacccaa tattctcttc cagcatgtgt tgcataactt caaagcgcat
     1261 acgttctatc agagattgat ggtaaaatag gtagcgttta tcaaatggac gatacaatat
     1321 ttctttgata taaatgtcaa gatcactgat gtttgctact tcttgccggg aatctttgat
     1381 cttccaaccg aatttctctt tcaaattaaa tgcctgtaaa atgatttcgt ccggatgagc
     1441 aagattttgt aattgtaata ttctgttttt taaatccctt ttatcaaaac taatgacaaa
     1501 atgatcgcgc gccgtaacaa tacctacact attcaccgga aaaatatcag taatttgttt
     1561 ccatttcaaa tactgcttta cttttcctgt atctatcttt ttaaagaaat aataagggct
     1621 tgtcggttta attttctcat aattgttctt ttgaaaatcg tttttttgca gccattgata
     1681 cttttcttca cgcagtccat ataaatctgc gtgaaatacg ctagattcct tcggcttttt
     1741 atttttcaca aataacgcaa ttgccacgcc ctgacgaata tcaaacacat
//
LOCUS       DAOOMU010000197         1773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_85477_length_1773_cov_24.644936, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000197 DAOOMU010000000
VERSION     DAOOMU010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85477_length_1773_cov_24.644936"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="PLI55_10120"
     CDS             <1..291
                     /locus_tag="PLI55_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01226.1"
                     /translation="ANLVDIIANAVKVPLMLSQQTVRIYTSPALFLQDNSTFWFRFFA
                     NLDLFVLWKVILLGIGVGIITKVKTSKSIWTILICWIIYCLAVAGLGGLAKV"
     gene            314..1618
                     /locus_tag="PLI55_10125"
     CDS             314..1618
                     /locus_tag="PLI55_10125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPB01227.1"
                     /translation="MKRVIFILLIGQILAYGADSLSLKDCINLAISQNQDLKISNKQL
                     QMAEEGIRSTYSAVLPSVSFDYSATKSTVGAGERFYQGVYIPTKDTSSNYFSTGLSLN
                     QNLYNGGKRRNQIKLAKGEAESSRLNLNRTQQAIIANVTEKFYTVLKAKELLKVYEMT
                     LKNSQEQLKKTEEMYKIGQVAKKDILKAQVSEGESRLNLIKQQTSLKNALNDLKMAIG
                     LASDYALDVYEDSYLKPEIIDQAVAEQKALENNPELKSLQIQKTNSYLNYKIAQGDLW
                     PSVNGSFSYGRDGSEIERTYSEFDKWWRTSLNLNISYPLFTGFNRKANIQQKKINYNL
                     YDDQIEKKKLEIISEVERLILTINTNLEMIAINEINILSAQEDLRLAQEMYRLNSATL
                     LDVLDAQVAFTKAQGNLVTTKYDAKIAEVQLAYLMGILQQPE"
BASE COUNT          577 a          335 c          382 g          479 t
ORIGIN      
        1 gccaatctgg tggacattat cgctaatgcg gtaaaagttc cacttatgtt atctcagcag
       61 actgtgcgga tttataccag cccggcttta tttctccagg acaactccac tttttggttt
      121 aggtttttcg ccaatcttga cctttttgtt ctctggaaag tgattttact gggtatcggt
      181 gtgggaataa taactaaggt gaaaaccagt aagagtatct ggaccatcct catttgctgg
      241 atcatttatt gtctggcggt tgccggtctg ggcggcttag ccaaagtata acataggttt
      301 taggttggta gctatgaaaa gagtaatatt tattttatta attgggcaaa ttctggcata
      361 cggtgccgat tcattgtcat tgaaggattg cataaatttg gcaatatctc aaaatcagga
      421 cttaaaaata agtaataaac aattgcagat ggcggaagaa ggtatccgca gtacctatag
      481 tgctgtacta ccttctgtca gttttgatta ttcggcgact aaatccactg tgggagcagg
      541 tgaacgattt taccaaggcg tttacattcc aactaaagac acgtcttcca attatttcag
      601 cacgggtctt tctctcaatc agaatcttta caatggcggc aaacgacgga atcagataaa
      661 actcgccaaa ggggaagcgg agagttctcg attgaatctc aatcgcactc aacaagccat
      721 tattgccaat gtcaccgaaa aattctatac agttttaaag gctaaagaat tattgaaagt
      781 ctatgaaatg actttgaaaa acagccagga acaattgaaa aaaacggaag aaatgtacaa
      841 aatcggacag gttgccaaaa aagacatact caaagcccag gtcagcgaag gtgagagccg
      901 tctgaatttg attaaacagc aaacctcatt gaaaaatgct ttgaacgatt taaaaatggc
      961 aattggtctc gcgtctgatt atgctctgga tgtttacgaa gattcatacc tcaagcctga
     1021 gataattgac caggctgtag ccgagcaaaa agcgctggaa aataatcccg agttgaaatc
     1081 cctccaaatt cagaaaacga actcctattt gaattataaa atcgcgcaag gcgatctctg
     1141 gccgtcggta aacggctctt tttcttatgg gcgagacggt tcggaaatcg agcgtactta
     1201 cagtgaattc gataaatggt ggagaacttc gcttaacctg aatatcagtt acccactttt
     1261 taccggcttt aatcgaaaag cgaacatcca gcaaaagaaa atcaactata atctttatga
     1321 tgaccagata gaaaagaaaa aactggaaat tatcagtgaa gtagagaggc taattctgac
     1381 cattaatacc aatctggaaa tgatcgcaat caacgagatc aatatcctat cggcgcagga
     1441 ggatttgcgt ttggcgcagg agatgtaccg tttaaattcg gctacgcttc tggatgtgct
     1501 ggatgcccag gttgctttca ccaaagctca gggaaatttg gtcacgacta aatatgatgc
     1561 caaaatcgct gaagtccagc tggcatattt aatgggaata ttgcaacaac cggagtgaat
     1621 tatgaaaaaa ggcggaaaga aaattttaat aacagtaata gtaattatcg tcattggtgc
     1681 aatcgttttg ctcaatatga ataagaagaa agaaaaacca ctggcggttc aaacggagaa
     1741 agtcaagcgt caaaccatcg ttcagactgt caa
//
LOCUS       DAOOMU010000198         1765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_86086_length_1765_cov_15.269006, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000198 DAOOMU010000000
VERSION     DAOOMU010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86086_length_1765_cov_15.269006"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLI55_10130"
     CDS             complement(<1..531)
                     /locus_tag="PLI55_10130"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01228.1"
                     /translation="MNVNRISLRFVVEYRLIKIVRRILRSLSLSERRMLADITGNFVY
                     VFLPYRRATMRDNLKRAFPESTARWRRRIIRKCYVHFARVYFDFFPLFEASDTQLDAM
                     VECCDLNILHNALARRRGVVLVSFHFGNWEVCGDWFRRHNYQIAVVAKKMKNHLVDRM
                     IFDARSQSGRNKEQIFY"
     gene            complement(598..1224)
                     /locus_tag="PLI55_10135"
     CDS             complement(598..1224)
                     /locus_tag="PLI55_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01229.1"
                     /translation="MHKITLWLGCILLFGNLFAQEPAVPEVIIQESDTSETALPDSAT
                     EAMPQVEIPTVPLTPAQLPSETLTEEPAQPEPAPEELKPPEEVLPVLVASQKAKMKPS
                     EITHFGQMIYWKSLTPSEKKVFLYAYLYHTYEILEQIKHDRNLKSCSKQFQQKIADPV
                     FDIFAQLTDSQKDDLIFWIDKFYRIDSNKDQPFSEALRYANEKMKTGS"
     gene            complement(1236..>1765)
                     /locus_tag="PLI55_10140"
     CDS             complement(1236..>1765)
                     /locus_tag="PLI55_10140"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPB01230.1"
                     /translation="ASLMIAANRLFSEKYDYPLHLGVTEAGPVWSGTIKSAIGIGTLL
                     AEGIGDTLRVSLTANPVEEVRAGFEILKALDLTKSGIRLVSCPTCGRTSVNLIEIVNE
                     VESKIRDIPKNLTVAVMGCEVNGPGEARSADLGIAFGKGVALLFKHGQVVARLKPEEA
                     VARLIAEIENWSPTQ"
BASE COUNT          486 a          406 c          394 g          479 t
ORIGIN      
        1 gtaaaatatt tgctctttat tcctgccgct ctgacttctg gcatcaaaaa tcatccggtc
       61 aacaagatga tttttcatct ttttagcgac aactgcaatc tgatagttat gacgcctgaa
      121 ccaatcgcca cagacttccc aattgccaaa atgaaaagag accagcacta cgccgcggcg
      181 acgggctaag gcattatgta aaatatttag gtcgcaacat tctaccatag catcaagttg
      241 agtatcagat gcttcaaaaa gcgggaagaa atcgaaatac acccgcgcaa aatgaacata
      301 acacttgcga ataatccggc gccgccagcg ggcggtggac tccggaaaag cccgcttcaa
      361 attatctcgc attgtcgccc ggcgataagg tagaaaaaca tacacaaagt taccggtgat
      421 atcagccaac atacgacgct ctgacagcga caaagagcgc agaatgcggc gaacgatttt
      481 tatcagtcgg tactcaacaa caaaacgcag tgaaattcta ttaacattca tcactggatt
      541 ttataagctg gttaatcttt ggattcacta ggagttttct ttcaaaagtt gcattaatta
      601 agaacccgtt ttcattttct cgttggcgta gcggagagct tcactaaagg gctggtcttt
      661 gttagaatca atccgataaa atttatcaat ccagaaaatc aagtcgtctt tttgactgtc
      721 ggtcaattga gcgaagatat cgaaaacagg atcggcaatt ttctgttgga attgtttgga
      781 acaggatttg aggtttcgat catgctttat ctgttccaag atttcatagg tatgatagag
      841 ataggcatat aaaaagactt ttttctcgga aggagtcagc gatttccagt aaatcatttg
      901 cccgaagtgg gtaatttcag atggtttcat tttggctttt tgggatgcaa ccagaaccgg
      961 gagaacttcc tccggtggtt ttagttcttc cggagctggt tctggctgag ctggttcctc
     1021 ggtaagcgtt tctgaaggca attgagccgg ggtcaatggt actgtgggaa tttcgacctg
     1081 aggcattgct tcagtagctg aatccggcag tgcggtttca gatgtatcac tttcttgaat
     1141 aatcacttcc ggcactgccg gttcctgagc gaacaggtta ccaaataata aaatacatcc
     1201 aagccagagt gtgattttat gcataatggg acctcctatt gcgtcgggct ccaattttca
     1261 atttccgcta tcaatcgcgc taccgcctct tccggtttca atcgtgcgac aacctgaccg
     1321 tgtttaaata atagagctac tcctttaccg aaagcaattc ccagatcagc cgaccgggct
     1381 tcaccggggc cattgacctc acagcccata accgcaacag ttaagttttt aggtatgtcg
     1441 cggattttac tttcaacttc gttcactatt tcaatcagat taaccgaagt gcggccgcag
     1501 gtgggacacg aaaccagccg gatgccgctt ttagtcaaat ccagcgcttt gagtatttca
     1561 aaaccggcgc gtacttcttc gactggattc gccgtcagag acacccgcaa agtatcgccg
     1621 attccttcag ctaaaagcgt tccgatacca atggcggatt taattgtacc cgaccagacc
     1681 ggaccggctt cggtgacacc gagatgaagg ggataatcat atttctcgga aaagagccga
     1741 ttagcggcga tcatcagact ggctt
//
LOCUS       DAOOMU010000199         1753 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_87013_length_1753_cov_10.752061, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000199 DAOOMU010000000
VERSION     DAOOMU010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87013_length_1753_cov_10.752061"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1297)
                     /locus_tag="PLI55_10145"
     CDS             complement(89..1297)
                     /locus_tag="PLI55_10145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPB01231.1"
                     /translation="MAKLIDYRGIVGDKVITGIFQKARGLYGKKIIHLNSTYMGGGVA
                     EILNSLVPLMNDVGLDTGWRIIRGNPDLFTITKKFHNALQGDPINLSDMKKEIYIKAS
                     EDFSVFTHLDHDCVIIHDPQPLPLIRYYVKQQPWIWRCHVDISHPNPVLWDFLKAFIL
                     KYDLAIVSHETYKKDDIYIEQKVIPPVIDPLSPKNKELSEKDTKRFLKKFGIPIDKPL
                     LTQISRFDKWKDPEGVVRIFKKVKKQIDCRLVLCGSMATDDPEGMKIYQKVVDYSNHL
                     IQSGDIIPITSENNILVNSLQRYSDVVIQKSIREGFGLVVAEALWKKAAVVASNVGGI
                     PLQVIDGETGFLCEPNDEEAFAERIIQLMKDKKLKEKLGQNGKEHIKSNFLITRLLCD
                     YLDVIRSLLS"
     gene            complement(1385..>1753)
                     /gene="otsB"
                     /locus_tag="PLI55_10150"
     CDS             complement(1385..>1753)
                     /gene="otsB"
                     /locus_tag="PLI55_10150"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HPB01232.1"
                     /translation="VAWHYRMAEPELGSLRAKELTDYLFNFIANIDIQVQQGKKVVEI
                     TNAGINKGAAGLQWIGKKDFDFILAIGDDWTDENLFEVLPERAYSIKVGMVETHARFS
                     LYNHTEVIELLKNLLKVNGN"
BASE COUNT          493 a          353 c          328 g          579 t
ORIGIN      
        1 ttattaagac tcaagatata agtgcgattt agattttctg cttggcgtgt ttttataata
       61 aaggaaataa atgagattag ctacagtgtc aggataataa cgatctgatt acatccagat
      121 aatcacaaag caggcgggta atgaggaagt tacttttgat atgctctttg ccgttttgcc
      181 ccagtttttc tttcagtttt ttatctttca ttaattgaat tatccgttcg gcgaaggcct
      241 cttcatcatt gggttcgcaa agaaaacccg tttcaccgtc aatgacctga aggggtattc
      301 caccgacatt ggaggcaact acagcggctt ttttccagag tgcttccgct actaccaatc
      361 caaacccttc tctgatagat ttctggatta cgacgtcgga ataacgctga agagaattga
      421 cgagaatgtt attttcggag gtaatgggaa taatatcccc ggattggatt agatgattag
      481 aatagtccac aactttctga tatattttca taccttccgg gtcgtcagta gccatactgc
      541 cacataaaac caaacggcaa tcaatctgtt ttttgacttt tttaaaaatt cttacgacgc
      601 cctccgggtc tttccactta tcgaagcgcg agatttgagt aagaagcggt ttatctattg
      661 gtattccaaa tttttttaaa aatcgcttgg tatccttttc ggacaattct ttatttttag
      721 ggctaagtgg gtcaatcacc ggtggaataa ccttttgttc gatataaata tcgtcttttt
      781 tataagtttc atgagataca attgctaaat cgtatttgag gataaacgcc ttcagaaaat
      841 cccataatac cggattggga tgcgaaatat caacgtgaca gcgccaaatc cagggttgtt
      901 gtttgacgta gtatctaatc aacgggagcg gttgtgggtc gtgaataatg acacaatcgt
      961 gatcgagatg agtaaaaacc gaaaagtcct cgctggcttt aatgtaaatt tcttttttca
     1021 tatcgctcag gttgatcgga tcgccctgga gtgcattatg gaattttttg gttatggtaa
     1081 ataaatcggg attgcctctg atgatccgcc aaccagtatc gagaccgacg tcgttcatca
     1141 acggtacgag actgtttaaa atttcagcca ctccaccgcc catataggta gaattcaggt
     1201 ggataatttt tttgccatat aaaccccgtg ctttttggaa tatgccagta attactttat
     1261 cgccgacaat tcctcgatag tcaattaatt tcgccatttt cttatcccct taattatagt
     1321 ttaggcttat tatgaattcc aatttatcga gactaaactt taatatgata ttactctcca
     1381 tttcttagtt tccattgact ttcaacaagt tcttgagcaa ttcaataact tcggtatgat
     1441 tatagagact aaacctggca tgagtttcaa ccataccaac cttgattgaa taagctcttt
     1501 cgggaagtac ttcgaatagg ttttcgtcag tccagtcatc gcctattgct aatataaaat
     1561 caaaatcctt ttttccaatc cattgaagtc cagcggcacc cttatttatg cctgcattag
     1621 taatttcaac cacttttttc ccctgttgca cctgaatatc aatattggcg ataaaattga
     1681 ataagtaatc ggtgagttct tttgcccgta aagaaccaag ttcaggttct gccatccgat
     1741 aatgccacgc cac
//
LOCUS       DAOOMU010000200         1750 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_87233_length_1750_cov_22.243658, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000200 DAOOMU010000000
VERSION     DAOOMU010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87233_length_1750_cov_22.243658"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PLI55_10155"
     CDS             complement(<1..1322)
                     /locus_tag="PLI55_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB01233.1"
                     /translation="MTFLNFLFPPDDPLSDILKKISTVAECNHKRVFLVGGVVRDRLL
                     GIDTKDIDISVIGNGIEFAHKLAKSLKVDNVIEYPRFGTAMIPYHDKLIEVATARMEE
                     YETSSRKPQITESSLRDDLARRDFTINAMALSLNRENYLELTDYFEGLKDLNAGIIRT
                     PLDPITTFSEDPLRMLRAIRFATQLNFRIEPNTFKAIEQVRERIKIISQERITDEVTK
                     ILMVPENPSRGFELLQESTLLDLILPEIAALDGVDQRNGYHHKDVFKHTLQVLDNIAQ
                     VSSKFELRLTALLHDVAKPLTKQFFPDKGWTFYGHEELGAQMIGEIGKRLRLPNKTIE
                     YAAKLTHLHLRPIAIASEGITDSAVRRLIVEAGEELDDLILLCRADITSKNVQKVKTY
                     RANFDRVVNLIESVREKDRLRAFQSPVRGEEIMRFCNLPPGPAVGFIKS"
     gene            complement(1557..>1750)
                     /locus_tag="PLI55_10160"
     CDS             complement(1557..>1750)
                     /locus_tag="PLI55_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01234.1"
                     /translation="EGKNPFQLESKEPNGKLMEFLDGEVRYATLKKSFPEESEKLRKA
                     LSEEVMERYQRYKRMAEGS"
BASE COUNT          471 a          404 c          373 g          502 t
ORIGIN      
        1 gatttgataa atccgaccgc tggcccgggc ggcagattac agaaccgcat aatttcctca
       61 ccgcgtaccg gcgattggaa agcccgcaga cggtcttttt cccgcacgct ctcgattaga
      121 ttgacgaccc ggtcaaaatt ggctcgatag gttttaacct tctgaacatt ttttgaagta
      181 atgtctgccc gacacagcaa tatcaaatca tctaattcct ctccggcttc gacgatgagg
      241 cgtcgcacag cactgtcggt aattccttcc gaggcgatag cgatcggccg caaatgcaga
      301 tgggtcagtt tggcggcgta ttcgattgtt ttgttcggta agcggagtcg cttcccgatt
      361 tcaccaatca tttgcgcgcc caattcttca tgcccgtaaa atgtccagcc cttgtcggga
      421 aaaaattgct tagttaaagg cttggctacg tcatgcaaga gagccgttag acgcaattca
      481 aattttgaac tcacctgggc aatattgtcc agcacctgta aagtatgctt aaaaacatct
      541 ttatggtgat agccgttgcg ttgatcaact ccatcaagag ccgcgatttc cggtagaatc
      601 aaatccagca aagtagattc ctgcagtagt tcaaaaccgc gcgaggggtt ttctggcacc
      661 atcaaaattt tggtaacttc atcagtgatg cgttcctgcg aaataatttt gattcgctcg
      721 cgaacctgct ctatagcttt gaaagtattg ggctcgatcc gaaaattcaa ctgcgtggcg
      781 aaacggatgg ctcttagcat tctcagcggg tcttcggaaa aagtggtaat cgggtccagc
      841 ggagtacgaa taattccggc attgaggtct tttagacctt cgaaataatc cgtcaactct
      901 aggtaatttt cccgattcaa ggaaagtgcc atcgcattga tcgtaaaatc gcggcgggct
      961 aaatcatcac gtaaactact ttcggtgatc tgaggtttgc gcgacgaggt ttcgtattcc
     1021 tccatccgag ccgtagcaac ttcgatcaat ttatcatgat agggtatcat agcagtgcca
     1081 aagcgcgggt attcaataac attgtcaact ttcagacttt ttgccagttt gtgcgcaaat
     1141 tctatcccat taccgataac ggaaatatca atgtctttag tatcaattcc caataaccgg
     1201 tcacgcacca caccgccgac caaaaagacg cgcttatgat tacactcagc cacggtggag
     1261 attttcttta aaatatcaga cagaggatca tctggaggga ataaaaaatt aagaaatgtc
     1321 ataataaaac ttttaaaatg ctcgtcaatt taccaacctg gtcagttaat cacaatacta
     1381 atcgaagaac aaatgttcca cgaatacgga tttaattttg acggcctgga tgtatggatg
     1441 cgtggatgag tagagattga attagaatag ccattctgat ttcttataag cacttataag
     1501 cacttaagag aacttaatat ccgagcccct ttctcattgg agtcgttata ttaaacttag
     1561 cttccttccg ccattcgttt atatctctga tagcgctcca ttacttcttc ggatagggct
     1621 tttctcaact tttccgattc ctctgggaaa gatttcttga gggtggcata acgcacttcg
     1681 ccatctaaaa attccattag cttaccattg ggttccttgc tttcgagctg gaacggattt
     1741 ttaccttcgg
//
LOCUS       DAOOMU010000201         1731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_88696_length_1731_cov_10.612172, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000201 DAOOMU010000000
VERSION     DAOOMU010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88696_length_1731_cov_10.612172"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            394..1584
                     /locus_tag="PLI55_10165"
     CDS             394..1584
                     /locus_tag="PLI55_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB01235.1"
                     /translation="MVELKTDQIKFRNVITKIVKYLQSPEAIVITGMRRTGKTTILNY
                     FYQQIESENKLYLDLENPINRMYFEESNYEKIKTSFEVLGLDFSQKSFVFLDEIQFVA
                     NLPSVVKYFIDHYRIKFFLTGSASFYLKNLFTESLSGRKVIFELFPLTFREFLIFKDS
                     SLKIPDDSKKVTRSIFDTIIPLYNEYLTFGGFPGVVLKSNADEKKRALEEIFTSFFQM
                     EVVQLGDFRKNDVIRDLILLLMQRVGTKLDVQKLSNELGVSRTTLNEHLSFLESTYFI
                     KRVRPFSKGRDYEIRKAPKVYLCDSGLANHFAKLSPGTIFENSVFQNLITNGEVNYYQ
                     KKSGVEIDFILNKKVAYEVKMTPDESDVRRLKHLSQELGIEEFKIISGNYTSLENALY
                     GFMI"
BASE COUNT          561 a          304 c          327 g          539 t
ORIGIN      
        1 tccctatccc agtctttatt attagccaat aatgccttct caatctttca ttgatcccgc
       61 tttggcggga tttcctaccg gaacgatccc gccgtagcgg gagaagcaat ccccatcccg
      121 taagattcat aagcctttac caacctgaat aattgaattt tcagcctctt acgagacttt
      181 ttgccaccaa ggcaccaaga cacaaaaaaa ttagatttaa ggtggagata gagagaaagg
      241 aaatcattga aagcccttga cttaagtcgg cgttagacaa cttgagttaa gggctttaaa
      301 ataaatcagg caaaggttaa atccgtgatc aaatgaccaa attaatatta tactttacat
      361 ttggttcaac tgtttgtata ttagacttag tatatggttg agttaaaaac tgatcaaatc
      421 aaattcagaa atgtcataac gaagattgtc aaatatctcc aatctccgga ggcaattgtt
      481 attaccggga tgcgaagaac tggtaaaacc acaatcctaa attatttcta tcaacaaata
      541 gaatcagaaa acaaactata tcttgacctg gaaaatccca tcaataggat gtatttcgaa
      601 gaatctaact atgaaaaaat taaaacctca tttgaggtcc tgggactaga tttttcccaa
      661 aaatcctttg tattccttga cgaaattcaa tttgttgcta atcttccatc agtcgttaaa
      721 tattttattg accactatcg gataaaattt ttccttaccg gttcagccag tttttatttg
      781 aaaaatttat ttactgagtc attatcgggc agaaaagtta tttttgaatt atttccgttg
      841 actttcaggg aatttttaat atttaaagat tcatcgttga aaatacccga tgattccaag
      901 aaggttactc gatcaatctt cgatacaata atacctcttt ataatgagta tcttactttc
      961 ggcggattcc ccggagtagt attaaaatct aacgctgatg agaaaaagag agccttggag
     1021 gaaattttta cctcattttt ccagatggaa gttgttcagc ttggtgattt cagaaagaat
     1081 gatgtaatca gggatctgat cctacttcta atgcaaaggg ttggtacaaa gttggatgtt
     1141 cagaaactat ctaacgagct gggtgtttct cggactacct tgaatgaaca tctgtccttt
     1201 ctggaaagca cctattttat caaaagagtg cgacctttca gtaaggggcg ggattatgaa
     1261 attaggaaag cgccaaaagt ctatctttgc gactccggac tggcaaatca ctttgccaaa
     1321 ttgagtcctg gaaccatttt cgaaaactct gtttttcaga acttgataac taatggtgaa
     1381 gtcaattatt atcagaaaaa gagtggcgta gaaattgatt ttattcttaa taaaaaagtc
     1441 gcctacgaag taaaaatgac tcccgatgaa tctgatgtca gacgattaaa gcatttatct
     1501 caagaattag ggatagaaga atttaagatt atttccggaa attacacttc tttagaaaat
     1561 gccttatacg gttttatgat ttaatgggtt aatgttgagt ttgctaatga catattaatc
     1621 gtgattaagg catagggcgt atggagtaca actgtcaagg atcgttaaaa gtgtacattt
     1681 ggagtgcacc gagcatttgg agtgcaccga ccgtgtcggt gcatgcagtg a
//
LOCUS       DAOOMU010000202         1726 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_89103_length_1726_cov_14.055057, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000202 DAOOMU010000000
VERSION     DAOOMU010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89103_length_1726_cov_14.055057"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLI55_10170"
     CDS             <1..189
                     /locus_tag="PLI55_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01236.1"
                     /translation="RKVTIIINKTLRSLRPSRLKKNLTRQVRQEPPMTKISNYSISII
                     SYDSEQYLIKLFNVNEEY"
     gene            192..1463
                     /locus_tag="PLI55_10175"
     CDS             192..1463
                     /locus_tag="PLI55_10175"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB01237.1"
                     /translation="MLSKDQIKQILADQHATILNKSLGIERTLLSDIDKTIKLPQVVV
                     ITGLRRVGKSTLMRQIIKKYYNDQHFYYINFEDERLFKFKAEDFNLLYEALIELYGEH
                     QTFFIDEIQNIEHFENFVRRFYDSGFKFFITGSSARLLSREIGTKLTGRHIDMVVRPF
                     SFCEYLSLKEVSLEKEMLYNTVERTKIKSHFQNYLVSGGMPEYLLYNEPEILSRIYED
                     IVIKDIAVRYNVANLSEMRDIYLYLITNVANKFSFNKLKTISGVGSITTIKNYLHYLS
                     ETYFLQVINKFDYSLKKQMVNDKKAYIIDNGFFNVLSTKITQDTGWLLENLVSNVLSA
                     NYRLYYFSDKYECDFVVQKENRIVSAIQVTAEINNINEKREYNGLLNALKTFNLKEGL
                     ILTNDQESTIQIEDKTIHIKPVWKWLVEAER"
     gene            1497..>1726
                     /locus_tag="PLI55_10180"
     CDS             1497..>1726
                     /locus_tag="PLI55_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPB01238.1"
                     /translation="MKFERFEEIIAWQKAKEMTTQIYNLFKDNKDYGFKDQIQRASVS
                     VMNNIAEGYERKSNTEFRQFLYIAKGSCGEVRS"
BASE COUNT          599 a          317 c          303 g          507 t
ORIGIN      
        1 cgcaaagtaa caataataat taataaaact ttgcgttctt tgcgtccttc gcggttaaag
       61 aaaaacctaa cccgccaagt acgtcaggag ccgccgatga ccaaaatttc gaattactca
      121 atctctataa tatcttatga cagtgaacaa tatcttatta aattgttcaa tgttaatgaa
      181 gaatattaat aatgctcagt aaagaccaaa tcaaacagat tctagctgac caacacgcaa
      241 ctattctcaa taaatctctg ggaatagaac gtaccctgct atccgatatt gataaaacaa
      301 tcaaacttcc tcaggttgtc gtcattaccg gcctgcgccg cgttggtaag tcaacgctaa
      361 tgcgccagat tattaaaaag tactacaacg atcagcattt ttactatatc aattttgaag
      421 atgaacgctt gtttaagttt aaagctgaag atttcaatct tctctatgaa gccctcattg
      481 aattatatgg cgaacaccaa accttcttca ttgacgaaat tcaaaacatt gaacactttg
      541 aaaattttgt tcgccgtttt tacgactccg gctttaagtt tttcattacc ggctcaagcg
      601 ctcgtcttct tagccgcgaa atcggcacca aactaaccgg gcggcatatt gatatggttg
      661 tacgtccctt ctcgttctgc gaatatctat ctctaaaaga ggtttcttta gagaaagaga
      721 tgctgtataa cactgtcgaa cggactaaaa ttaaatccca ttttcagaat tatcttgtta
      781 gtggcggtat gccggaatat cttctctata acgaacccga gattctcagc cgcatttatg
      841 aagatatcgt gataaaagat attgctgtcc gttataacgt tgccaatcta tctgaaatgc
      901 gcgatattta tctttacctc atcaccaatg tcgctaataa attcagtttc aataaactca
      961 aaacgatcag tggcgtaggc agtatcacta caattaaaaa ttatcttcat tacttatctg
     1021 aaacctattt tcttcaggta attaataagt ttgattatag tcttaaaaag caaatggtaa
     1081 atgataaaaa ggcttacatc atagacaatg gcttcttcaa cgttctttcc acaaaaataa
     1141 cacaagatac cggttggtta ttggaaaatc tggtgagtaa tgttctgagt gctaactatc
     1201 ggctgtacta ctttagtgat aaatatgaat gcgatttcgt cgttcaaaaa gagaatagaa
     1261 tcgtctccgc tatccaggtt actgctgaaa taaacaatat aaatgaaaaa cgcgaatata
     1321 acggattatt gaatgctttg aaaaccttta atctcaaaga gggactcatt cttaccaatg
     1381 atcaggaatc aacaattcaa atagaggata aaactatcca tatcaaacca gtctggaaat
     1441 ggctggtgga agccgagaga tgaaagttaa aagttagaaa gtttataaag ttaaaagtga
     1501 agtttgagcg attcgaagaa ataattgcgt ggcaaaaagc aaaggagatg acgactcaaa
     1561 tatataattt attcaaagat aataaagatt acggatttaa ggatcaaatc caaagagcat
     1621 cagtatctgt tatgaataat atcgcggaag gttatgagag gaaaagtaat acagaatttc
     1681 gccagtttct atacatagcc aaaggatcgt gtggagaagt tcgatc
//
LOCUS       DAOOMU010000203         1711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_90286_length_1711_cov_4.635870, whole genome shotgun sequence.
ACCESSION   DAOOMU010000203 DAOOMU010000000
VERSION     DAOOMU010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90286_length_1711_cov_4.635870"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(419..583)
                     /locus_tag="PLI55_10185"
     CDS             complement(419..583)
                     /locus_tag="PLI55_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01239.1"
                     /translation="MRCWDSTDARTATPTRLPFEVLEQLAQKITRTVPGVVSVTYNVT
                     SKPPSTIEAV"
     gene            complement(590..1381)
                     /locus_tag="PLI55_10190"
     CDS             complement(590..1381)
                     /locus_tag="PLI55_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPB01240.1"
                     /translation="MIKEITAQELDTESFIRTQIIEIQRIVGNGQAINALSGGVDSST
                     VTLLGHRALGERLKTVIIENGLMRAGEVEQVVSFFQKRGIRVEVIDVKAEFYAALKGI
                     TDPEAKREAITQTFYRDVFGRLVRESGAKHLLQGTILTDVDETVAGIKRQHNVFEQLG
                     IDSQVEFGYRILEPLIQLRKDGVRKVGKALGLSNEMFQRIPFPGPALATRVIGEVTPE
                     RIETVRQATQIVEELLANRGAFQYLAILHEDRVTGMCNNRREFSQ"
BASE COUNT          464 a          388 c          386 g          473 t
ORIGIN      
        1 aaaccggatt ttatcaacaa ctttatatgg atttatgaat taagcaggct aaataacctg
       61 tacctgcctg tcagcagaca ggaaggtggg ccattttttt atcttttgcc acactttttg
      121 agatgcttct gttgctttat atcccgacta aattacgccg tttttaggat cataagccct
      181 tttaaggcat gagactattt caccccgcag gttgtaaacg ccctgcgcaa tctgatcatc
      241 ttaaaccaga caaaaaaatt atcacgcttg gattgaacgg attgggacag gcttattctt
      301 taggctactg aatatttgcg aagtcaaaag tacaatccag gatgatatca actatatgta
      361 taaaaaatgg taaaacgaaa aaagaattct ggataattag tagccggaat cttgtttgtc
      421 agacagcttc aattgttgac ggcggcttgg aagtgacatt gtatgtaacg ctgacgactc
      481 cgggcacggt ccgagtaatt ttctgcgcca gttgttccag gacttcgaaa gggagccggg
      541 taggagtagc cgttcgcgca tcggtgctat cccaacaacg aatttcaatt tactggctga
      601 attcacgtcg attattgcac ataccggtca cgcgatcttc atgcaatatt gccagatact
      661 gaaaggcgcc gcgatttgct agtaactctt caacgatctg agttgcctgg cgaactgttt
      721 cgatgcgctc cggggtcact tcgccaatca cccttgttgc cagcgccggt ccgggaaaag
      781 gaatgcgctg gaacatttca ttggacaatc cgagggcttt gccaaccttt ctgacgccat
      841 ctttacgcag ttggatgagc ggttcaagga tgcggtagcc aaactcgacc tgcgaatcga
      901 ttcccaactg ttcaaagaca ttatgctggc gcttgatacc ggctacggtc tcatctacgt
      961 cggtcagaat agtgccttgt agtaaatgtt tcgcgccgct ttcgcgcacc agccggccga
     1021 aaacgtcacg gtaaaaagtc tgagtaatcg cctcacgttt ggcttccgga tcggtaatcc
     1081 ctttaagcgc cgcgtagaat tcggctttga catcgattac ttctactctg atgccgcgct
     1141 tttggaaaaa actgactacc tgttcgactt caccagcgcg catcagacca ttctcaatga
     1201 taactgtttt taaacgttcg cccaaagccc gatgaccgag caaggttacg gtggacgaat
     1261 cgactccgcc ggaaagcgca ttgattgcct gaccgttacc gacgatacgc tggatctcga
     1321 taatctgtgt cctgataaaa ctctctgtgt caagctcttg tgcggttatt tccttgatca
     1381 tatagtgttc tccttatagg acggtatttt aatagatttt gtcgagtaat taaagaacta
     1441 agcaaaaact ccgactacaa atttcggaaa tgaatattat gccatatcct aaaagattaa
     1501 cttataaaat ggaagccagg gaaagaatga aatagtacgt gagttcgatt cttagggatt
     1561 tgtccggccg ttgccggact ctaccgactg agttaactaa aaacaattat ttaaaaacta
     1621 tggaattgtt aaagattagt ggcgaggaac ggaattcccg cctgaccgat agacatcagt
     1681 cagttgggta ggatcgccga tctcgccata g
//
LOCUS       DAOOMU010000204         1683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_92471_length_1683_cov_14.382064, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000204 DAOOMU010000000
VERSION     DAOOMU010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92471_length_1683_cov_14.382064"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLI55_10195"
     CDS             <1..750
                     /locus_tag="PLI55_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01241.1"
                     /translation="FIAALTGALAISGIPPLNGFYSKWLIYQGLIEKMQNLTAGYQIW
                     LLICLILAVFGSALTLASFMKFIHAIFLGKRPKKWKDVKEAPFNQWFSVGVLALLCVV
                     FGLFAKPLPIGKFIAPIISELGLPTPVILGSYEPVQLFLLFGTAFLLGLVIYLLVKNV
                     RFDENYLGGMPSLEKFRIAGTEFYNEIRNISPLKSIYNAAEKKHFDVYDLGVRGSLSF
                     SGLLKRLHSGQLLNYNLWIIIGMLILLWVVW"
     gene            783..1334
                     /locus_tag="PLI55_10200"
     CDS             783..1334
                     /locus_tag="PLI55_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HPB01242.1"
                     /translation="MPTETWLIFILGFMLVGSVAALEIKDLLSAIIAVGIVGLGLSMA
                     FLLLQAPDLAIVQFLFEIFAIVIMVRAFMKPGYHFSDTKSKHRGRTIIVMAVLVAIFI
                     LSINVFKALPPFGEPLMTTSQHYLENAARETGSANIVASIVLDYRGYDTLGEATVLFV
                     AVLGTLTILRVKSNKKKEEESEK"
     gene            1331..>1683
                     /locus_tag="PLI55_10205"
     CDS             1331..>1683
                     /locus_tag="PLI55_10205"
                     /inference="COORDINATES: protein motif:HMM:NF015970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HPB01243.1"
                     /translation="MKQEPGLTLIAKTVTRVSVWLIILYGIYIILHGHLSPGGGFAGG
                     VVIALAFLNVMLTYGRETVTHWVNVEFLHDLEPACGAIYMAIGILGISISGGFLANFL
                     SKGTLFNLLSSGNIL"
BASE COUNT          408 a          313 c          421 g          541 t
ORIGIN      
        1 ttcatcgcgg ctttgaccgg ggcgctggcg atttcgggaa ttccgccgct aaacggtttt
       61 tactcgaaat ggctgatcta ccagggatta atcgaaaaaa tgcagaattt gacagccggt
      121 tatcaaattt ggctcttgat ttgcctgata ctggcggttt tcggaagtgc tttgacctta
      181 gccagcttta tgaagttcat tcatgctatt tttctcggga aacgtcctaa gaaatggaaa
      241 gacgttaaag aggcgccttt taatcagtgg ttttccgtgg gtgtattagc cttgttatgt
      301 gttgtttttg gattattcgc taagcctttg ccgatcggga aatttattgc tccgataata
      361 tccgaacttg gtttgccaac gccggttatt cttgggtcat acgaaccggt gcagttgttt
      421 ctgctgtttg gtacggcatt cctgctgggt ttagttattt atctgctggt caaaaacgtt
      481 cggtttgacg aaaattatct tggcgggatg ccgtctctgg aaaaattccg cattgccggg
      541 acggagtttt ataatgaaat caggaatata tctcctttaa agtcgattta caatgcggct
      601 gaaaagaagc attttgatgt ttacgacctt ggagtgcgcg gctctttgag tttttccgga
      661 cttctaaagc gcctgcatag cggccaattg ctaaactata atctctggat tattatcgga
      721 atgttaattc tgctatgggt ggtgtggtga tgttaacgat tgaatatttt atggaggaat
      781 aaatgccgac tgaaacttgg ttaattttca ttttaggttt tatgttagtg ggttcggtcg
      841 cggcgctcga aatcaaggac ttgctatcgg ctattattgc ggtgggaatt gtcgggttgg
      901 gactgtctat ggcatttttg cttttacagg cgcccgacct ggcaattgtc cagtttctat
      961 tcgagatttt tgcgatagtg ataatggtgc gagcctttat gaaaccggga tatcatttca
     1021 gcgatacgaa atccaaacat aggggtagaa caattatcgt aatggcagtt ctggttgcca
     1081 tttttatatt aagcatcaat gttttcaaag ccttgccgcc ctttggcgag ccattgatga
     1141 ccacttcgca acactatttg gaaaatgccg ccagagaaac cggttcagcc aatatcgtgg
     1201 cttcgatagt tctggattac cgcggttatg atacgctggg agaagccacc gtattattcg
     1261 tagcggtttt aggaacgctg actattctaa gggtgaaatc gaataagaaa aaagaagagg
     1321 aaagcgaaaa atgaaacaag aacctggttt aacattaatc gccaaaaccg tgacgcgggt
     1381 cagtgtctgg ttgatcattc tctacggaat ctatataatt ttgcacggtc atttgagtcc
     1441 cggtggcggt tttgctggcg gggtggtgat cgcattagca tttctcaatg tgatgttaac
     1501 ctatggtcgc gaaaccgtta cgcactgggt caatgtcgaa tttctgcacg atttggaacc
     1561 agcctgcggt gcgatatata tggcgattgg cattttagga atttctatca gcggcggttt
     1621 tttagccaat tttttaagta aagggacgct ttttaatctg ctcagttccg ggaatattct
     1681 att
//
LOCUS       DAOOMU010000205         1652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_95308_length_1652_cov_27.309330, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000205 DAOOMU010000000
VERSION     DAOOMU010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95308_length_1652_cov_27.309330"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="PLI55_10210"
     CDS             complement(<1..539)
                     /locus_tag="PLI55_10210"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPB01244.1"
                     /translation="MDNFDLIVIGSGPGGYVAAIRAAQLGMKVALVEKAELGGICLNW
                     GCIPTKALLKNAEVWQNLRTLDEFGITIAQPQLDFTQVIRRSREVAARLSKGIEFLFK
                     KNKVAVVRGYGELRDRQTVAVVNPDDNSTTGLKARNIVIATGARPRSIPNVEIDGKRV
                     ISSREAMILPQIPKSIIIIG"
     gene            complement(552..1400)
                     /locus_tag="PLI55_10215"
     CDS             complement(552..1400)
                     /locus_tag="PLI55_10215"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPB01245.1"
                     /translation="MPYLGCHVSIENSLEAAPMRGRELGCDAIQIFTSNQMQWKGVPV
                     ADETAEKFLNYQAEHKIAIVIAHDSYLVNLASPEPVKLAMSRKAFIEEIRRSQALKIP
                     YIVFHPGSHMGAGVDFALRKIAESLDYAIEKTPDCRPMFLLETTAGQGTNVGNTFEEL
                     RQIIDYSAHPERLGICFDTCHAYAAGYDLVSPAKYDETFARFDDVIGLSRLKCFHLND
                     SKKPLGSRVDRHANLGDGFLGWEVFYRLINDDRFGNLPMILETPGGDENFAKEIASLK
                     KALEKK"
BASE COUNT          434 a          403 c          373 g          442 t
ORIGIN      
        1 ccgatgatga taatggattt cggaatctgc ggcaaaatca tagcttcgcg actactgata
       61 acgcgttttc cgtcaatttc gacattagga atcgagcgcg ggcgggcgcc ggtggcaatt
      121 acgatatttc gcgccttgag cccggtggtg gaattatcgt ccggattaac gaccgcgacc
      181 gtctgccggt cgcgcaattc gccataacct cggacgactg cgactttatt tttcttaaat
      241 agaaactcga tgcctttcga aaggcgagcg gcgacctcac gactgcggcg gataacttgg
      301 gtgaaatcga gttgcggttg agctattgtg atcccgaatt cgtccaacgt gcgcagattc
      361 tgccagactt ccgcattttt gagcaaggct ttggtcggga tacagcccca gttcaggcat
      421 attccgccga gctcggcttt ttcaaccaat gcgaccttca ttccaagctg ggcggcgcgg
      481 atagcggcta cgtatccgcc cggtcctgat ccaatgacta tcaaatcaaa attatccatg
      541 gttattcctt attatttttt ctccaatgcc tttttcagag aggcaatttc tttagcaaag
      601 ttctcgtcgc cgccgggcgt ctccagaatc atcggcaaat tcccaaaacg gtcatcattt
      661 atcaaacgat aaaaaacttc ccagcccaga aaaccgtctc caagattggc atggcggtca
      721 acgcgcgacc cgagcggttt tttgctatcg tttaggtgaa agcatttcag tcggcttaaa
      781 ccgattacgt cgtcgaatct ggcaaaagtt tcatcgtatt ttgccggact aaccagatcg
      841 tatccagccg cataagcatg acaggtgtcg aaacaaatcc ccagtcgttc tggatgagca
      901 gaataatcaa taatctggcg caattcctcg aaagtgttac caacattcgt gccctgaccg
      961 gcagtcgttt ccagtagaaa catcggacga caatccggcg ttttttcaat ggcgtagtcg
     1021 agactctcgg cgattttacg caaagcaaaa tccacaccgg cacccatatg cgaaccggga
     1081 tgaaaaacga tatacggaat ttttagtgct tgcgaacgcc gaatctcctc aataaaagcc
     1141 ttgcgcgaca tcgccaattt aaccggctcc ggacttgcca gattgaccag ataagagtcg
     1201 tgagcaatta ctatcgcaat cttatgttcg gcttgatagt tcaggaattt ctccgccgtt
     1261 tcgtctgcaa ccggaacgcc tttccattgc atttgattgg aagtaaaaat ctggatcgcg
     1321 tcacagccca gctcacggcc gcgcatcgga gctgcttcca gactgttttc gatcgagaca
     1381 tgacaaccga gataaggcat ttttttagtt acgagtcagt ggttaattgc ggttctattt
     1441 tcacggctaa ctgcgaagta tttttattgt aataagagca agttccattt gttcgcgcga
     1501 atttagataa aattcggatt aatcgaaaga tttccgcaat agttaccttg caataataag
     1561 gtcaaacaaa atcctgacag gatagaaatt atctcctcta aaatatctgt gcctcgttga
     1621 ggtaaaacat tataaaaatt ggggattaat ta
//
LOCUS       DAOOMU010000206         1618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_98382_length_1618_cov_14.085733, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000206 DAOOMU010000000
VERSION     DAOOMU010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98382_length_1618_cov_14.085733"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..1608)
                     /gene="tviB"
                     /locus_tag="PLI55_10220"
     CDS             complement(328..1608)
                     /gene="tviB"
                     /locus_tag="PLI55_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012140193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HPB01246.1"
                     /translation="MSIIGLGYVGLPLAVEFATKFPVTGFDIKKERVEELRNGHDATL
                     EVEDDHLKSVLINQNGRTGLFLTDQLDDIRPCNYYIVTVPTPIDQYKRPDLTPVIKAT
                     ETVSRILKKGDIVIYESTVYPGCTEEVCVPILEKGSGLHFNTDFFCGFSPERINPGDK
                     VHTVTKIKKVTSGSTPEIARKIDALYQTIITAGTFLASSIKVAEAAKVIENTQRDLNI
                     AFVNELAKLFNMMSIDTKEVLEAAGTKWNFLPFQPGLVGGHCIGVDPYYLTFKAQGIG
                     YHPEVILAGRRINDSMGFYVAEQVVKLMIKKGHTINASQVLILGITFKENCPDVRNSR
                     VIDIITELQQYGCRVDVYDPWADSQEVKREYGIDLVAQFSQKMGNKQLPHYDAIVLAV
                     AHNEFKTLNLKPLKNNRTVIYDVKGILAEYDGRL"
BASE COUNT          441 a          338 c          341 g          498 t
ORIGIN      
        1 atggcacagc cccgtcaact acattcttcg acggagcata gttatattct ggtttctcac
       61 ggctaaatta agccgactca aatcatcagt gagttattgt caggaacagc ttttcagggc
      121 attcgtttca ggcattttga gtgacatact ttaataattc tttcccattt cctgtgacaa
      181 gctaattgat aatttaaatt aataaatcat agttaggatg tcaatcaaat tttaatcctt
      241 taagatcgaa ccaatgttta gttgagattg atgtgcgctg aatcactttt tacctactct
      301 tgcgattatc tcagaaaaag tatcttgcta taaccttccg tcgtattccg ccaggattcc
      361 tttgacatca tagataacgg ttcgattatt tttcaatggc tttaagttca atgttttaaa
      421 ttcattgtga gcaactgcca atacgatagc atcataatgg ggaagttgct tattacccat
      481 tttttggctg aattgcgcga ctaaatctat tccgtattca cgtttgactt cctgggaatc
      541 cgcccaggga tcgtaaacat caacccgaca tccatattgt tgaagttcag taataatgtc
      601 aatgactcga gaattgcgca catcggggca attttcctta aaggtgatac ccagaatcaa
      661 tacctgcgag gcattaattg tgtgcccttt tttgatcatc agtttgacga cttgttcagc
      721 cacataaaaa cccattgaat cgttgattct tcgtcccgct aaaatgactt ccggatgata
      781 gcctatgccc tgagctttaa atgttaaata atatggatca acgccgatgc aatgcccacc
      841 aaccaaaccg ggttggaaag gcagaaaatt ccatttagtc ccagccgctt ctaatacttc
      901 tttagtatca atgctcatca tattgaaaag cttggcgagt tcgttgacaa acgcaatgtt
      961 gaggtcgcgc tgagtattct caatcacttt agcggcttcg gcaactttta tagacgaagc
     1021 caggaaagtt ccggcagtga tgatggtttg gtaaagagca tcaattttgc gagcaatttc
     1081 gggtgtggaa ccggaagtaa cttttttaat tttagtaacg gtatgaactt tgtcgccggg
     1141 attaatgcgt tccggagaga agccgcagaa aaagtcggta ttgaaatgca agccactgcc
     1201 tttctcgaga attggtacgc aaacctcttc agtgcaaccc gggtaaaccg tggattcata
     1261 aatgacaata tcgccttttt ttagtatgcg actaactgtt tcggtggctt tgatgaccgg
     1321 tgttaaatca ggacgcttgt attgatcaat gggagtaggc accgtgacga tgtaataatt
     1381 gcagggtcga atatcgtcga gttgatcggt taagaacaga ccagttcgcc cgttttggtt
     1441 aataaggacc gacttcaggt ggtcgtcttc aacttccagg gtggcatcgt ggccatttcg
     1501 taattcttcc actcgttctt tcttaatatc gaatccggtt acgggaaatt ttgtagcaaa
     1561 ctcgactgcc agcggcaagc cgacgtaacc aagtccaatg atgctgattc tgtcggta
//
LOCUS       DAOOMU010000207         1617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_98499_length_1617_cov_3.512804, whole genome shotgun sequence.
ACCESSION   DAOOMU010000207 DAOOMU010000000
VERSION     DAOOMU010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98499_length_1617_cov_3.512804"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLI55_10225"
     CDS             <1..294
                     /locus_tag="PLI55_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HPB01247.1"
                     /translation="PNPYVVTNTWEPKNPYSSGRSERQLHFIHLPAKCTIKIFNVRGQ
                     LVDTIKRDSQLDDGTEIWDMLSKDNLEIAYGIYIYHVQAEGIGEKTGKFVVVK"
     gene            348..1364
                     /locus_tag="PLI55_10230"
     CDS             348..1364
                     /locus_tag="PLI55_10230"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HPB01248.1"
                     /translation="MKSKIFLFAGLLLATSQMLAGVTKVGTTAAGFLMIDVGSRANGM
                     GSAFVSIADDATAMYWNPAGIAGIDGFEAAFCNSKWIGDLGFTFTGAVQSLGQFGNIG
                     INATFLTMEEIERTTIAYPDGSGEYFDAGSYAVGLSYARNLTDNFSIGFNTKLINERM
                     YHSSASGFALDIGALFDTQLSGLKLGMCISNYGTKMHLDGRDLQVQHDISESISGNND
                     LINARLQTDYFDLPLLLRFGVSMDVLKGFMNSNLILSADALHPSDDTEYINLGAEYKL
                     FNLLSLRVGYKQLFAKDSEQGLTFGGGINYKLRGATAFYFDYSYSDFGLLNSVNIFSI
                     GLAF"
BASE COUNT          465 a          275 c          366 g          511 t
ORIGIN      
        1 cccaatccgt atgtcgttac aaatacctgg gaaccaaaaa atccctattc ttcaggtcgc
       61 agtgaaagac aacttcactt tattcatttg ccagccaagt gtactataaa aatttttaat
      121 gttagggggc agttggttga cacgataaaa cgggattccc aacttgatga cggaacggaa
      181 atctgggata tgttgtctaa agataatcta gaaattgctt atggcatcta tatttatcac
      241 gtccaggcgg aaggcatcgg tgaaaagacc ggtaaatttg tggtagttaa ataactaata
      301 aaataatgac cattaataga aatgttctct tatacggagt aatacaaatg aaaagtaaga
      361 tttttctatt tgccggattg ctattagcaa catctcaaat gctcgcggga gtgaccaaag
      421 ttggcactac ggcggccgga tttttgatga tagacgttgg ttcacgggcc aatggaatgg
      481 gaagcgcatt tgtttccata gctgatgacg ctaccgcaat gtattggaat ccagcgggca
      541 ttgccggaat tgatggcttt gaagcagctt tttgtaacag caagtggatt ggcgaccttg
      601 gatttacttt tactggcgca gtccagtcac ttgggcaatt cggtaatatc ggaataaacg
      661 cgacattttt aactatggaa gaaatcgaac gaactaccat cgcctatccg gatggatccg
      721 gtgagtattt cgatgccggt agttatgcag tcggattgag ttatgcccgt aatttgactg
      781 ataatttttc aatcggattt aataccaaac taatcaatga gaggatgtac cacagctccg
      841 ccagtggttt tgctctggat attggggcat tatttgatac ccagttatct ggtttaaaat
      901 taggcatgtg tatttccaac tatggcacca aaatgcactt ggacggacgt gatttgcaag
      961 tacaacatga tattagtgaa agtattagtg gcaataatga tctgattaat gcccgattgc
     1021 agaccgatta tttcgattta ccgctgctcc ttcgatttgg tgtgtcaatg gatgttctta
     1081 aaggttttat gaatagcaat ttgatcttat cggctgacgc tttacatccc agcgatgaca
     1141 ccgagtatat caatctggga gctgaatata agctttttaa tttgttatcg ttacgggttg
     1201 gatataagca actctttgct aaggactctg agcaaggatt gactttcggc gggggaatta
     1261 actacaaatt gagaggtgcg actgcttttt atttcgacta ttcttattcg gattttggtt
     1321 tacttaattc tgttaatata ttcagtattg ggctagcatt ttaagcctga ttaatctgga
     1381 tagtttataa aatggggcaa aattggaaat aaattttgcc ctatttattt atgcctaatc
     1441 aattatttta tttgtgctta gtagaatcta tggtttgttt agcataggtc aggtcgccgg
     1501 attggataag aaccggaaaa gctggaatcg tagtcggatg tggcataccg ggacggtagg
     1561 caaaaaaatc tacttttgta agggaaattt ggtcatatat tttccgatta atccaga
//
LOCUS       DAOOMU010000208         1616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_98571_length_1616_cov_22.392697, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000208 DAOOMU010000000
VERSION     DAOOMU010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98571_length_1616_cov_22.392697"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1616)
                     /locus_tag="PLI55_10235"
     CDS             complement(<1..>1616)
                     /locus_tag="PLI55_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB01249.1"
                     /translation="GNILGAVLVFRDITEQRKAKKELEFEKTLIDAMLNTFPDYIYFK
                     DLNSRFYRVSKSLATEVFGLPDPKDAIGKSDFDFFPPEFAQKDYNGEQEIIKTGKSII
                     DRSERKNWANGTVSWSSVTKIPLTDKDGQIIGTFGVSKNITESKKLEEALRQSEEKYR
                     KQFEESIDAIFLADAETGILIDCNKAAAKLVGREKSEIIGQHQRILHPPSEIINGQSR
                     TFLEHKKAKEGQTLESQIITKSGEIRDVEIKASVYEIENQKIVYALFHDVTEQKRIEA
                     ELAKERNYLRVLLNTTPDHIYYKDRDSRFLLMSKSQADRFGLSDPKEAIGKTDFDFFT
                     EEHARPAFEDEQRIIRTGQPIVGIEEKETWPDGGISWVSTTKMPLLDEKGEIIGTFGI
                     SRDITERKIAEEQLLKAMEEVKNSEDKLKNIINGSAIPQFFIDEDHKVVYWNTALTEL
                     TGIKAEEIVGTSNHWKAFYQEQRPCLADLLVEGNIKNLPEWYSDKYQKSHLVENGFEA
                     TEFFPNLGKDGKWLFFTAVAIKNSKGEIIG"
BASE COUNT          382 a          339 c          339 g          556 t
ORIGIN      
        1 tccgataatt tcaccttttg aattcttgat cgctaccgcc gtaaaaaata accatttccc
       61 atctttgcct aaatttggga aaaattcggt agcttcgaat ccgttttcga ctaaatgaga
      121 tttctggtat ttatcagaat accactcggg caagtttttt atatttccct ccacgagtaa
      181 gtcggcgaga cagggccttt gctcttgata aaaagctttc cagtgattgg atgtgccgac
      241 gatttcctct gcttttatgc cggttagttc ggtcaaagcg gtattccaat agacgacctt
      301 gtgatcttca tcgataaaaa attgggggat tgcagaacca ttaatgatat ttttgagctt
      361 atcctcactg ttttttactt cttccatagc ctttaataat tgttcttcgg ctattttacg
      421 ctcagtgatg tctcttgaaa ttccgaaagt gccgataatc tcgccttttt catcgagcaa
      481 cggcatttta gttgtcgaaa cccatgatat tcctccatct ggccaggttt ctttttcttc
      541 gattccgact atcggttgac cagtacgaat gatacgctgt tcgtcctcaa atgcaggtcg
      601 ggcatgttct tcagtgaaga aatcaaagtc ggtcttgccg atggcttcct tgggatcgga
      661 gagtccaaat cggtcagcct gggatttgct catcaataag aatcggctgt ctctgtcctt
      721 gtaataaata tggtcgggag tggtgttcaa taaaacgcgc aggtagttac gctctttagc
      781 gagttccgcc tcgattcgtt tctgttcggt aacgtcgtga aatagggcgt aaactatttt
      841 ttgattttcg atttcataaa cactggcttt gatttcgaca tctcggattt cgccgctctt
      901 agtgatgatc tggctttcta aagtttgtcc ctctttggct tttttatgct caagaaatgt
      961 tcgactttgc ccattgatga tttctgacgg aggatgcaga atccgctggt gttgtccgat
     1021 tatttccgac ttttctcgac cgactaattt ggcggcggct ttgttgcaat ctatcaagat
     1081 accggtttcg gcatcggcta agaaaatagc atcaatgctt tcttcgaatt gtttgcggta
     1141 tttttcttcg gattgtcgga gagcctcttc cagtttttta ctttcggtga tgtttttaga
     1201 aactccgaaa gtcccgatta tttgaccatc tttgtcggtg agtggaattt ttgtaactga
     1261 agaccaggag acggttccat tagcccaatt tttcctttct gatcggtcaa tgattgactt
     1321 gccagttttg atgatctctt gttcaccatt ataatctttt tgagcgaact caggggggaa
     1381 gaaatcgaaa tcgcttttgc caatcgcatc cttaggatct ggcaatccaa aaacctcggt
     1441 agctaaagat ttgctgaccc gataaaaacg gctatttaaa tccttgaaat agatataatc
     1501 agggaaggtg ttcagcattg cgtctattaa agttttttca aactcgagtt cttttttagc
     1561 tttgcgctgt tcggttatat cgcgaaaaac cagaactgcg cctaagatat tgccat
//
LOCUS       DAOOMU010000209         1605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_99610_length_1605_cov_12.201935, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000209 DAOOMU010000000
VERSION     DAOOMU010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99610_length_1605_cov_12.201935"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1605
                     /locus_tag="PLI55_10240"
     CDS             <1..>1605
                     /locus_tag="PLI55_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPB01250.1"
                     /translation="FENAAIGLYRSTPDGRILMANPTLVKMLGFDSFEELSQVNLEEG
                     GFDLKYPRSEFKKRIEAEGKVQNLESVWKRKDNSIIHVRESAIAVRDASGVTKYYEGT
                     VEDITARKKAEEELRESEKHYRELIDGMIETVWIIDFNGDLMDVNRTATEVLGYTKEE
                     LLKIGLFGIDSSLKREDIKTLARSMPKDKIQIFETTHKTKEGKSIPVEIYSSLIDYHG
                     QPAILSIARDITERKQAEEDLVRIMESAQAILWHSPVVRQEGTEPEFLWKTRYFNLDA
                     IKNIIPLPDYPTHDINDRYYYSILEEDRQAMDQVSSAALKSGADRYSQEFRLRDANGV
                     IHWMYEDARIKKIDESHFEVVGVITEVTERKKAETALKLSEERFKSLFEYNSSAVIIY
                     EVVDNGNDFIIRDINKAGEKIDKIKRSEVVGKSLLEVFSGVKDFGLFEVLQRVWRTGE
                     PENFPVTFYKDNRISGWRDNYVFKLPSGEVVAVYNDLTKQKQDEEERRKSEENFRLVW
                     ENSVDGMRLTDENGIVIMVNKAYCNL"
BASE COUNT          527 a          262 c          391 g          425 t
ORIGIN      
        1 tttttgaaaa tgcggcgatt gggctctatc gttctactcc ggatggcaga attttgatgg
       61 caaacccgac tctggtaaaa atgctcggct tcgattcttt tgaggaacta tcgcaagtga
      121 atctcgaaga gggaggattt gatcttaagt acccacggtc ggaatttaaa aaacgaatcg
      181 aagctgaagg aaaagtccag aatttagaat cagtatggaa gcgtaaagat aattcgatta
      241 ttcacgttcg cgaaagcgct attgcggttc gtgatgcgag cggtgtaact aaatattacg
      301 aaggcaccgt tgaggatatt acggcacgca aaaaggcgga agaagaatta agggaaagtg
      361 aaaaacacta tcgcgagtta attgatggaa tgatcgagac agtctggatt atagatttta
      421 acggcgatct tatggacgtt aaccgaactg caacggaagt cttaggatat actaaggaag
      481 aattactcaa gattggactt ttcggtattg attcctcatt aaaaagagaa gatatcaaga
      541 ctcttgcccg gtcaatgccc aaagataaaa tccagatatt tgaaactacc cacaaaacca
      601 aagaagggaa atctatcccg gtggaaatat attccagttt gattgattat cacggacaac
      661 ctgccatttt gagtattgcc agggacataa ccgaacgtaa acaagctgaa gaagatttag
      721 tccggataat ggaaagcgct caggctattc tctggcattc accggtagtc agacaggagg
      781 gaactgaacc ggaatttcta tggaaaactc gctatttcaa tctggacgcc attaaaaata
      841 ttatcccgct ccctgattat ccaactcatg atattaatga tcgttattat tacagtattt
      901 tagaagaaga ccgccaggca atggatcaag tcagttctgc tgcacttaag agcggtgccg
      961 atcgctatag ccaggaattt cgcttgcgag atgcaaatgg tgtaattcac tggatgtatg
     1021 aagacgcgcg aatcaagaaa attgatgaat cgcattttga ggtagttggt gtcattactg
     1081 aggttacgga acgcaagaaa gccgagacag ccttaaaatt aagtgaagaa agattcaagt
     1141 cattgtttga atacaacagt agtgccgtaa ttatatatga agtggttgat aatggtaatg
     1201 atttcattat cagagatata aataaagccg gtgaaaaaat tgataagata aaaagaagtg
     1261 aggtagtcgg taagagctta ttggaagtat tttcgggagt gaaagatttt ggtttatttg
     1321 aagttttaca acgtgtctgg cggacgggtg aaccggagaa ttttccagtg acattttata
     1381 aggataatcg catctccggc tggagagata actatgtttt taaactgccg agcggcgaag
     1441 ttgtagcggt ttataatgac ctaactaaac aaaagcagga tgaagaagag aggcgcaagt
     1501 ctgaggagaa ttttcggttg gtatgggaaa attccgtaga tggtatgcgc ctgaccgatg
     1561 aaaatggtat agtcataatg gtcaacaagg cttattgcaa tctgg
//
LOCUS       DAOOMU010000210         1563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_103641_length_1563_cov_10.750000, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000210 DAOOMU010000000
VERSION     DAOOMU010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103641_length_1563_cov_10.750000"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1563)
                     /locus_tag="PLI55_10245"
     CDS             complement(<1..>1563)
                     /locus_tag="PLI55_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01251.1"
                     /translation="LADEIGQDIDYFGNKVDPLFDAFGEHIDSVFTGTADFEFRVKIM
                     GSKYQDDMFVFSRTFFNRMEMIGELGETMAETMGDGFNQIIDSLNNNSTAIDPGVAVV
                     QEGLDSLNALIDNVQVLLLNQPFAPLELNLAWMDSLQKGINEFDAILAGKTYPIGPES
                     ENKVIRPRGILESLAHPDGPMGVFQDFYRGGENQSYTFYNTFPRGITTKMYAMIASDI
                     ILNSYDTREEFEARIHSYQADLKQKPSLNPDEHFGLALTLLYDLSGEFFGNVESAIQI
                     ISEGRIDSLADTYNWSDFDVQDQIDEIRYHVDQYIESEEPTNFVILTKEYGYSQDPYA
                     DPNPYVLDANSEFSLTFLAVPQVKIATAAIEEACEGMSKIGDVLNGLYNELDQIFILD
                     LDPNYLDFSEAKGPMDVINILEQSNPEFLTVTDYGVTKFHEMGDWLEGSFNSLWIFFN
                     NLSGLFNAMRPYQDDFGIDAAQMQGISTMSAEMAWALYRDFANPDSTIIIDGERVNVS
                     AWFDNPPTSFLV"
BASE COUNT          477 a          358 c          294 g          434 t
ORIGIN      
        1 taaccaggaa acttgtcggc ggattatcga accaggctga aacattgact cgctcgccgt
       61 caatgataat agtggaatcc gggttcgcaa aatcacgata gagagcccaa gccatttcgg
      121 ccgacatagt cgaaattcct tgcatttgag cggcatctat cccaaagtca tcctgatagg
      181 gtcgcatcgc attaaaaagt ccactcagat tattgaagaa aatccacaaa ctattgaacg
      241 aaccctcgag ccaatcgccc atttcatgaa atttcgtgac gccataatca gtaacagtta
      301 aaaattcggg attagattgt tccaatatgt tgatcacatc catcggtccc ttagcttcgg
      361 aaaaatccaa gtaatttggg tcgagatcca aaatgaatat ctgatcgagt tcgttatata
      421 aaccgtttaa aacatcaccg atttttgaca taccttcgca agcctcttca atcgcggcag
      481 tggcaatttt tacctgtgga acggcaagaa aagtgagtga aaattcactg ttggcatcta
      541 aaacataagg attcggatct gcgtatggat cctgactata tccgtactct ttcgtgagaa
      601 taacaaaatt ggtcggttcc tcactctcga tatattgatc aacatgatag cgaatttcgt
      661 caatctgatc ctgcacgtcg aagtcactcc agttgtaggt atccgctaaa ctatcaatgc
      721 gcccttccga aattatctga atggcactct ctacatttcc gaagaattct ccgctcaaat
      781 cgtaaagcag agtcaacgcc agaccaaaat gctcatccgg gtttaggcta ggtttctgct
      841 tcaaatcagc ctgataagaa tggatacgcg cttcaaattc ttctcgagta tcataactat
      901 tcaagataat atctgaagca atcatagcat acattttagt cgttatacct cgcggaaatg
      961 tgttatagaa agtgtaagac tggttttccc cgccgcgata gaaatcctgg aaaacgccca
     1021 ttggtccatc gggatgggct aggctttcga gaataccgcg cggacgaatg actttatttt
     1081 cagattcggg gccaatcgga taagtcttac cagctaaaat cgcgtcgaat tcgttaattc
     1141 ccttttgtag agaatccatc caggctagat taagttcaag cggagcaaat ggctgattta
     1201 aaagtaacac ttgaacattg tcaattaaag catttaggga atccagtcct tcttgaacaa
     1261 cggctactcc cggatcaata gccgtactgt tgttatttag cgaatctata atctggttaa
     1321 atccatcacc cattgtttca gccatagtct cgcccaattc accaatcatt tccatacgat
     1381 tgaaaaatgt tctggaaaat acgaacatat cgtcttgata ttttgaaccc ataatcttaa
     1441 ccctaaattc aaaatcagcc gtaccggtaa atacggaatc aatatgctca ccgaaagcat
     1501 cgaacaacgg atctacttta ttgccaaaat aatcaatgtc ctgtccaatc tcatcggcta
     1561 gtt
//
LOCUS       DAOOMU010000211         1529 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_107136_length_1529_cov_19.848033, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000211 DAOOMU010000000
VERSION     DAOOMU010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107136_length_1529_cov_19.848033"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLI55_10250"
     CDS             complement(<1..293)
                     /locus_tag="PLI55_10250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPB01252.1"
                     /translation="MPKKILVIDDDVDLIEVLRLMLEKNGYLVIDAQNGTRGYQLVEK
                     ERPDLIILDVMMATWDEGFEVVQKIRNNPKLAATPIIMLTAVSQQMGIRYAKD"
     gene            complement(344..1021)
                     /gene="hypB"
                     /locus_tag="PLI55_10255"
     CDS             complement(344..1021)
                     /gene="hypB"
                     /locus_tag="PLI55_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPB01253.1"
                     /translation="MEIKIVRHVLEANDKLAAEIRQRTANSKTLLVNLMSSPGSGKTT
                     LLEKLIPMLQSQGYKIGVIEGDITTTMDAERLQPLNIPIVQINTEPFGGDCHLGAEVI
                     MPALDSLDLANLDFVFIENVGNLVCPAEFDTGANVNVVVLSVTEGEDKPLKYPLMFRV
                     CQLALISKTDLLPYLNLNLDLLRQNMLRINPNLTIFPISAQRGDGLADLRNWLIEYKR
                     WISDVAE"
     gene            complement(1137..1490)
                     /locus_tag="PLI55_10260"
     CDS             complement(1137..1490)
                     /locus_tag="PLI55_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HPB01254.1"
                     /translation="MHELKIAQNIIAIAADELAQRQISTAVRVIHFKAGQMHAIIPET
                     LLFNFDTIKSEIPQFAHAVLSIEIIPLRIKCRQCQNISELDEPVFECRHCHGTDLEII
                     NGQEMFVDNIEMEDE"
BASE COUNT          382 a          315 c          362 g          470 t
ORIGIN      
        1 ttatctttgg catatcgaat gcccatttgc tggctaacgg cagttagcat aataatagga
       61 gtagcggcta atttgggatt attgcggatt ttttggacaa cttcaaatcc ctcgtcccag
      121 gttgccatca taacgtcgag aatgatgaga tcggggcgtt ctttttcgac taattgatag
      181 ccgcgggtgc cgttttgggc atctattacg agatagccat ttttctccag catcaaacgc
      241 aggacttcta ttaggtcaac atcgtcgtct ataactaata ttttcttagg cattactttc
      301 ctcatttcat attaataagc gttcgaagtt aaaatgcttg accttattct gcaacatctg
      361 aaatccagcg tttgtattca attagccaat ttcgcagatc ggcaaggccg tctccgcgct
      421 gagctgaaat cgggaaaatg gtgagattgg gattgattct caacatattt tggcgtagta
      481 aatccagatt cagattgagg taggggagta ggtcggtttt actgattaat gccagctggc
      541 acactcgaaa cattagtgga tatttgagcg gcttgtcttc accttcggtc acactcagaa
      601 cgaccacatt gacattggcg ccggtatcga attccgccgg gcaaaccaga ttaccgacat
      661 tttcaataaa aacgaaatcg agattagcca gatcgagact atcgagcgct ggcataatta
      721 cttccgctcc gagatgacag tcgccgccaa atggttcggt attgatttgt acaatgggaa
      781 tgttcaaagg ctgaagacgc tcggcatcca tcgtggtggt gatgtcgcct tcgatcacgc
      841 cgattttata gccttgcgac tgaagcatcg gaatcaattt ttccagcagg gtggttttgc
      901 cggagccggg tgaactcatt aaattgacga gtaatgtttt tgaatttgca gtgcgctgac
      961 ggatttcagc ggctaatttg tcattggctt cgagaacgtg gcgcacaatc ttgatttcca
     1021 tagtttgatc cttttgggtt acacggaggc gcacagagga ttcactgagg ttcacggagt
     1081 aatttttata attattaaaa tctctgtgtt cctctgtgtc ataattactt ctatcgctat
     1141 tcatcttcca tctcgatatt gtcaacgaac atttcttggc cgttgattat ttccagatcg
     1201 gttccgtgac aatgccggca ctcgaatacc ggttcgtcga gttcggaaat gttttgacat
     1261 tgacggcatt tgatccgtaa cggaattatt tcgatagaca gaacggcgtg tgcgaattgc
     1321 ggaatttcag attttatggt gtcgaaattg aataggagtg tttccggtat aatggcatgc
     1381 atctgaccgg ctttgaagtg gatgaccctg acagccgtgg aaatctgacg ttgagccagt
     1441 tcatcggcgg cgatagcgat tatattttgg gcgattttta gttcatgcat tgctttctcg
     1501 taaaaccgtt aggaataatc tctcgcaaa
//
LOCUS       DAOOMU010000212         1507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_109584_length_1507_cov_14.504132, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000212 DAOOMU010000000
VERSION     DAOOMU010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109584_length_1507_cov_14.504132"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLI55_10265"
     CDS             <1..425
                     /locus_tag="PLI55_10265"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPB01255.1"
                     /translation="GVASNIVPGSCYIYLDIRSVPGQTEEILLEQIQECIAKVSDGNN
                     FKVEIISTNQPHAISPDGELVRKIQSNAKDFLNIEPQAIGQGGGTYAKTLCLNGIAAV
                     GWGPGDDNAFHVANEYVEIKQLVDFALMTCLLAVDLLV"
     regulatory      480..603
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            588..>1507
                     /gene="ribD"
                     /locus_tag="PLI55_10270"
     CDS             588..>1507
                     /gene="ribD"
                     /locus_tag="PLI55_10270"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPB01256.1"
                     /translation="MREDEKSRAQSGFFMNQNEKWLKRALELAEKGRGFTSPNPVVGA
                     VIVSGEKVVGEGFHSEFGAPHAEIKALEQAGESARGARLYVNLEPCCFYGKTPPCTEA
                     IIKSGISEVYIGTIDPNPNVNGAGVKSLEQAGLKVQVGILEREARSANRGFFTFIQKK
                     RPWITLKMALTLDGFIADATGKSRWITGVEAREFVHKQRQQYDAVVVGMGTVYKDDPE
                     LLPENRSGYIPYRVVLDEALNLPPRMKLVTDGFRQRTVIVTACQEKAEKIRQFGSAGV
                     KIIQSSKDEFGWLDLPKTLAKLAEFGVTSI"
BASE COUNT          403 a          293 c          401 g          410 t
ORIGIN      
        1 gcggagtggc ttctaatatt gtgcccggtt cttgctatat ttacctcgat atacgttctg
       61 tgccgggtca gacggaggag attctattag agcagattca ggagtgcata gccaaagtca
      121 gtgatggtaa taattttaaa gttgaaatta tttccaccaa tcaacctcac gccattagcc
      181 cggatggcga attggtccga aaaatccaat ccaatgccaa agattttctc aatatcgaac
      241 cgcaagccat tggtcagggc ggcggtacct atgccaaaac gctttgtttg aacggtattg
      301 cggcagttgg ctggggaccg ggtgacgata atgcgtttca cgtcgccaat gaatatgtgg
      361 aaattaaaca gctagtagat tttgcgttga tgacctgtct gctggcggta gatttgctgg
      421 tttgaactcg aaaacatttt tacagtattg gaacttcgtt gattgtatta taaatttgtg
      481 ggcgtcttcg gggcagggtg taaatcctga ccggcggtga aagtccgcga gttccgttga
      541 ggcgggacag agtctgtgaa actcagacac cgacggtaaa agtccggatg agagaagatg
      601 aaaaatcccg agcgcaatcg ggttttttta tgaatcaaaa tgagaaatgg ctaaaacgag
      661 ctctggaact ggcggaaaaa gggcgtgggt ttaccagtcc taatccggta gtaggagccg
      721 tgattgtctc tggcgaaaaa gttgtcggtg aaggtttcca cagtgaattc ggtgctcctc
      781 atgccgaaat taaagcgtta gagcaagccg gtgaatccgc gcgtggtgcg agattatacg
      841 ttaatctcga accgtgttgt ttctatggca agacgccacc ttgtactgag gctattatta
      901 aatcaggaat tagtgaagtt tatatcggta caatcgatcc caatcccaat gttaacggcg
      961 ctggagttaa gagtctcgaa caagccggtc taaaagttca agtcggtatt ttggaaaggg
     1021 aagctcgctc tgccaatcgc ggctttttta cctttattca aaaaaaacgt ccttggatta
     1081 cgctgaaaat ggctctgact ttggatggtt ttatagccga tgctaccggt aaaagccgct
     1141 ggattaccgg tgttgaagcg cgggagtttg ttcataagca aagacaacaa tatgatgcgg
     1201 tggtagttgg aatgggcacg gtttacaaag acgacccgga gttacttccc gagaatcgtt
     1261 ccggatatat tccttatcga gtagtactgg atgaagcttt gaatctgccg ccgcgaatga
     1321 aattagtaac tgatggtttc cgacagcgaa cggtaatcgt taccgcttgt caagaaaaag
     1381 cagagaagat tcgccagttc gggtcggccg gtgtgaaaat tattcagagt tcgaaagacg
     1441 aatttggttg gctggatttg ccgaaaactc ttgctaaact ggctgagttt ggcgttactt
     1501 ccatttt
//
LOCUS       DAOOMU010000213         1502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_110164_length_1502_cov_17.632343, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000213 DAOOMU010000000
VERSION     DAOOMU010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110164_length_1502_cov_17.632343"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PLI55_10275"
     CDS             <1..227
                     /locus_tag="PLI55_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPB01257.1"
                     /translation="GNVTFFAFDEGQRLSEHTAPFDALVQILDGEAEITIAGQPFSVK
                     ADEMIIMPANQPHAVKAIQRFKMLLTMIKG"
     gene            complement(352..>1502)
                     /locus_tag="PLI55_10280"
     CDS             complement(352..>1502)
                     /locus_tag="PLI55_10280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPB01258.1"
                     /translation="LLRHHPWLFSGAIERIEGEPQLGETVELRAHDGRFLGFGAWSPK
                     SQIAVRIWSFNPDDEINADFLRRQLSWAIKRRESLFEPGKTDAVRLVNAESDGLPGLI
                     VDRYNDYLVVQFLSAGAEFWRETIVTVLAEIVPVTGIYERSDVEVREKEGLELRRGLL
                     QGNEPSELIEICENSRRFLVDIRNGHKTGFYLDQRDNRQKITEFSARREVLNCFAYTG
                     GFALAALSAGATKVTNVETSAEALELLNRNVALNGFNPAKVENLTDDVFQVLRKFRDQ
                     GRSFDLIILDPPKFAESKSQIERASRGYKDINLLAFKLLRPGGILFTFSCSGQISPEL
                     FQKIVADSALDAGKSAQILVWLNQSADHPTALNFPEGHYLKGLVCVVD"
BASE COUNT          410 a          382 c          347 g          363 t
ORIGIN      
        1 ctggaaacgt gactttcttc gccttcgacg aaggtcagcg actgagcgaa cacaccgcgc
       61 ctttcgatgc actggtgcaa attctcgacg gcgaagccga aatcacgatt gccggtcaac
      121 cgttcagcgt caaagccgac gaaatgatta ttatgcccgc caatcagccg cacgccgtga
      181 aagccatcca acgcttcaaa atgttgttga cgatgattaa aggataaaac tcaccgtcta
      241 tagtaatcga ataagtctcg attattatcc ggatcagggt aaaaatcatt tctctcgcaa
      301 acttgcggtg ctactcttta tcttaatatt gcgactgaag tttcacgtct atcaatcaac
      361 aacgcagacc aatcccttga gataatgacc ttcggggaag tttaaagccg tcgggtgatc
      421 ggccgactga ttgagccaga ccaagatttg ggctgatttt ccggcatcga gggcagaatc
      481 agctacgatt ttctgaaata attccggcga tatctggcct gaacaagaga aggtaaaaag
      541 tataccgccc ggccgtaaaa gcttaaacgc cagcaggttg atgtccttat agccgcgact
      601 ggcacgttca atttgacttt tcgactcggc aaatttggga ggatcgagga taattaaatc
      661 gaatgaccgt ccttgatcac gaaactttcg cagaacttga aaaacatcat cagtcaggtt
      721 ctcgacctta gccggattga aaccattcag ggcaacattg cggttcagta attcgagagc
      781 ttcggcggaa gtttcgacat tcgttacttt ggtggcaccg gctgacaaag ccgcgagcgc
      841 aaatccaccg gtataagcaa aacaattcag cacttcgcgc ctagctgaga actcggtgat
      901 cttttgtcgg ttgtcgcgct ggtcgaggta aaaaccagtc ttgtgaccat tgcgaatatc
      961 aactaaaaaa cgccgagagt tttcgcagat ttcgattaat tccgatggtt cgtttccttg
     1021 taataaaccc cgacgaagtt ccaaaccttc tttttcgcgc acttcaacat ccgaacgctc
     1081 ataaattccg gtaaccggga cgatttcggc cagcaccgtg acgatcgtct cacgccagaa
     1141 ttcagctccg gctgaaagga attgaactac cagataatcg ttataacgat caacgatcag
     1201 tcctggtaaa ccgtcggatt cggcgttgac caagcggact gcatcagttt tgcccggctc
     1261 aaaaagcgac tcgcggcgtt tgatcgccca actcaattga cgtcgcaaaa agtcagcgtt
     1321 gatttcatca tccggattga aactccagat gcgcacggca atttgagact ttggcgacca
     1381 ggcgccgaat cctagaaaac gaccgtcatg tgcccgtaac tcgacggttt cacccaattg
     1441 cggttcgcct tcaattcttt caatcgcacc tgaaaagagc cacggatggt gacgcaggag
     1501 cg
//
LOCUS       DAOOMU010000214         1499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_110515_length_1499_cov_18.022161, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000214 DAOOMU010000000
VERSION     DAOOMU010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110515_length_1499_cov_18.022161"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1263
                     /gene="rlmN"
                     /locus_tag="PLI55_10285"
     CDS             199..1263
                     /gene="rlmN"
                     /locus_tag="PLI55_10285"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPB01259.1"
                     /translation="MFHMRTPAKINLKGLNLEEITSLLAALGEKPYRARQIYQWLYQK
                     RATDWSQMSNIPLALRTKLAERFELSSVRLQNLVHSRIDGSVKFLFELTDGNLIEAVY
                     MPEDRRRTVCLSSQVGCPIGCAFCATGVMGLKRNLTAGEIIDQLLFINANMPVPATNV
                     VFMGMGEPFLNYENVIRAARIFNSELGPELSARHITISTCGVIPGIERFTAEDQRFKL
                     AISLNATTDEQRDKIIPINRKYPIKELLGVAKNYTRVSRRRVTFEYILIAGLNDTIKD
                     AQRLVKLLSPIPCKLNLIPYNDNQQFPYRAPSEEHLNEFIREIYQAPFAVTVRRSKGL
                     DIAAACGQLYVTEQNKRNLR"
BASE COUNT          425 a          347 c          294 g          433 t
ORIGIN      
        1 caggacggat agagaattca attccaaaat gggaatcatt aactaaagat acaacctctt
       61 gccaaaatta acccgaaaaa gcaattgtgg aaatcctatt atttttgtcc aaaacttttc
      121 acaacttttt atagaatact ggttaatgaa aaaattagac ttttccgaat caatctgatt
      181 aacttttgat gatttttaat gtttcatatg cgcacaccgg ctaaaataaa tctgaaggga
      241 cttaatctgg aagaaatcac ttcgctcctg gccgcattgg gtgaaaaacc gtaccgcgct
      301 cgccagattt atcaatggct ttatcaaaaa agagctaccg attggagtca aatgtccaat
      361 attcctctgg cgcttcgtac gaagttggct gagcgatttg aattgagctc tgttcgtctg
      421 caaaatttgg tgcatagccg gattgatggt tcggtgaaat ttctattcga actaactgac
      481 ggtaatctga ttgaggcggt ctatatgccg gaagatcgcc gccgtaccgt ctgcctgtct
      541 tcgcaagtcg gctgtccaat cggatgtgct ttctgcgcaa ccggtgtgat gggcttgaaa
      601 cgcaatctaa ctgccggtga aatcattgac cagttacttt ttatcaatgc caatatgccc
      661 gttccggcaa ctaatgtggt ttttatgggt atgggcgaac cttttttgaa ttacgaaaat
      721 gtcattcgcg ccgcccggat tttcaatagc gagcttgggc cggaactttc cgcacgtcat
      781 attacaattt cgacctgtgg tgtcattccg ggcatcgagc gctttaccgc tgaagaccag
      841 cgctttaaac tggcaatttc cctcaatgcc actaccgatg aacagcggga taaaattatc
      901 cctatcaacc gcaaatatcc cataaaagaa ctgttgggtg tcgctaaaaa ttatactcgt
      961 gtctctcgac gccgggtaac ttttgaatat atcctgattg ccggattaaa cgatactata
     1021 aaagacgccc agcgcttggt caaattgctc agcccgatcc cctgcaagct caatcttatt
     1081 ccctataatg acaatcagca atttccatac cgcgccccaa gcgaggaaca cttaaacgaa
     1141 tttatccggg aaatttatca agcacccttt gcggtaaccg ttcgacgcag taaagggctg
     1201 gacatcgccg cggcttgtgg acaactatat gttaccgaac aaaataaacg caacttgcgt
     1261 taagccggac tggctactct ttgcctccaa accgaactta agtcaagcca aatagagtgg
     1321 atgaactcaa gttcttactt tccacccaat tattcataca aacaatccta caatcatgcg
     1381 ttcaggctta catttttatt atctggctaa aaatgtttgc aatgattatc aatgaatttg
     1441 caaaaattac tcttttttga aactttcctt gaccttctca atggcttctt cagtgctct
//
LOCUS       DAOOMU010000215         1485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_112141_length_1485_cov_15.539860, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000215 DAOOMU010000000
VERSION     DAOOMU010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112141_length_1485_cov_15.539860"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLI55_10290"
     CDS             <1..403
                     /locus_tag="PLI55_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPB01260.1"
                     /translation="VPGSCYIYLDIRSVPGQTPEILLEQIQECIAKVPNGNFKVEIIS
                     TNQPHAISPDVELVRKIQSNAKDFLNIEPQAIGQGGGTYAKTLCLNGIAAVGWGPGDD
                     NAFHVANEYVEIKQLVDFALMTCLLAVDLLV"
     regulatory      458..581
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            566..>1485
                     /gene="ribD"
                     /locus_tag="PLI55_10295"
     CDS             566..>1485
                     /gene="ribD"
                     /locus_tag="PLI55_10295"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPB01261.1"
                     /translation="MREDEKSRAQSGFFMNQNEKWLKRALELAEKGRGFTSPNPVVGA
                     VIVSGEKVVGEGFHSEFGAPHAEIKALEQAGESARGARLYVNLEPCCFYGKTPPCTEA
                     IIKSGISEVYIGTIDPNPNVNGAGVKSLEQAGLKVQVGILEREARSANRGFFTFIQKK
                     RPWITLKMALTLDGFIADATGKSRWITGVEAREFVHEQRRLHDAVMVGMGTVYQDDPE
                     LLPENRSGYIPYRVVLDEALNLPPRMKLVTDGFQRRTIIVTACQEKAEKIRQFESSGV
                     KIIQSSKDEFGWLDLPKTLAKLAEFGVTSI"
BASE COUNT          395 a          295 c          388 g          407 t
ORIGIN      
        1 tgtgcccggt tcttgctata tttacctcga tatacgatcc gtgccgggtc agacgccgga
       61 gattttatta gagcagattc aggagtgcat agccaaagtt cctaacggta attttaaagt
      121 tgaaattatt tctactaatc aacctcacgc cattagcccg gatgtcgaat tggtccgaaa
      181 aatccaatcc aacgccaaag attttctcaa tattgaaccg caagccattg gtcagggcgg
      241 cggtacctat gccaaaacgc tttgtctgaa cggtattgcg gcggttggct ggggaccggg
      301 tgacgataat gcgtttcacg tcgccaatga atatgtggaa attaaacagc tagtagattt
      361 tgcgttgatg acctgtctgc tggcggtaga tttgctggtt tgaactcgaa aacattttta
      421 cagtattgga acttcgttga ttgtattata aatttgtggg cgtcttcggg gcagggtgta
      481 aatcctgacc ggcggtgaaa gtccgcgagt tccgttgagg cgggacagag tctgtgaaac
      541 tcagacaccg acggtaaaag tccggatgag agaagatgaa aaatcccgag cgcaatcggg
      601 tttttttatg aatcaaaatg agaaatggct aaaacgagct ctggaactgg cggaaaaagg
      661 gcgtgggttt accagtccta atccggtagt aggagccgtg attgtctctg gcgaaaaagt
      721 tgtcggtgaa ggtttccaca gtgaattcgg tgctcctcat gccgaaatta aagcgttaga
      781 gcaagccggt gaatccgcgc gtggtgcgag attatacgtt aatctcgaac cgtgttgttt
      841 ctatggcaag acgccacctt gtactgaggc tattattaaa tcaggaatta gtgaagttta
      901 tatcggtaca atcgatccca atcccaatgt taacggcgct ggagttaaga gtctcgaaca
      961 agccggtcta aaagttcaag tcggtatttt ggaaagggaa gctcgctctg ccaatcgcgg
     1021 cttttttacc tttattcaaa aaaaacgtcc ttggattacg ctgaaaatgg ctctgacttt
     1081 ggatggtttt atagccgatg ctaccggtaa aagccgctgg attaccggtg ttgaagcgcg
     1141 ggagtttgtt cacgaacagc ggcgactaca cgatgccgtg atggtcggaa tgggcacggt
     1201 ttaccaagac gatccagaac tattgcctga gaatcgttct ggatatattc cttatcgagt
     1261 tgtgctggac gaagctttga atttgccacc gcggatgaaa ttagtaaccg acggttttca
     1321 acggcgaacg ataatcgtta ctgcttgtca agaaaaagcg gaaaaaattc gccagtttga
     1381 atcatccggt gtgaaaatta ttcagagttc gaaagatgaa tttggctggt tggatttgcc
     1441 gaaaactctt gctaaactgg ctgagtttgg cgttacttcc atttt
//
LOCUS       DAOOMU010000216         1452 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_116183_length_1452_cov_4.251969, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000216 DAOOMU010000000
VERSION     DAOOMU010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116183_length_1452_cov_4.251969"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="PLI55_10300"
     CDS             complement(<1..1428)
                     /locus_tag="PLI55_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01262.1"
                     /translation="MVYESEGHIWYEAKPPDGDWRLIKVPGNNGHPYLDVNGGSSPAM
                     DCYNIDDWNEYRVYISFKEGNYIQVYRFAYYSYANNYAYDGMCNLYIAESFYSPNIAS
                     SKDGFFMIVYKTSSGINYRLGAGEPGNGIWILPKYGIISGTNSNSESAAISGISLETG
                     WSSFDIAWLQLTPPPPAPPLVSVKYCQLEYDHLYNNVTYSAPPQTVSSSSMKINSNPS
                     IISLPDVPRIGWIADMSGTYDPWQTYATMCKIYPSISYNMYNYQSKSASINKLDNDSK
                     YYFTWSQVYYMYGWIKNNFAVCSTSPNVIKNLNTWGQDLQLCNGPNSSNMYVLSFYPY
                     STPYYFVKSNSLGSAGLGKTTEYPYSGKRGIVLENNNAGLYYSIGNIAVDGVSINFIP
                     NVDEISKRGNRDSVKYHGSKSIYNNTETVNGLLVSEPFDLKKDSQIEFNDYFGVADSV
                     AGVSVLGENGYITFKVELLDDASDKV"
BASE COUNT          458 a          301 c          255 g          438 t
ORIGIN      
        1 taccttatcg gacgcatcat ctaataattc caccttgaaa gtgatatagc cattttctcc
       61 aagaaccgat actccggcta cagaatccgc tactccgaaa taatcattga attcgatttg
      121 agaatccttt tttaagtcaa acggttcaga aactagcaac ccattaacag tttctgtgtt
      181 attataaata gatttacttc cgtgatattt tacactatca cgattgcctc ttttggatat
      241 ctcatcgaca tttggaataa aattaatgga cacgccatca actgcaatgt tgccaattga
      301 ataatacaat cccgcattgt tattctcaag aactatgcct cgcttgccac tataaggata
      361 ctccgtagtt ttacccaatc cggcgcttcc cagactgttg gatttcacaa aataatatgg
      421 tgtggaataa ggataaaaag ataaaacata catattactg ctgttgggtc cattgcaaag
      481 ttgaagatcc tgtccccaag tattaagatt tttaataaca ttagggcttg tgctgcaaac
      541 cgcgaagttg tttttaatcc agccatacat ataatagacc tgactccagg tgaaataata
      601 ttttgaatca ttatctagtt tattaatgga tgccgattta gattgataat tatacatatt
      661 ataagaaatg gatggatata ttttacacat ggttgcataa gtttgccagg gatcatatgt
      721 gccactcata tccgctatcc aaccaatgcg aggtacgtcc ggcagactga taatcgaggg
      781 attggaatta attttcattg aactacttga aacagtctga ggaggagctg aataggtgac
      841 attgttgtac aaatgatcat attctaattg acagtattta accgaaacca gcggcggggc
      901 aggtggtggt ggggttagct gtagccaggc tatatcaaaa cttgaccaac ctgtttccaa
      961 actaatacca gagattgccg cgctttcaga attgctgttt gttccactaa taataccata
     1021 ttttggtaaa atccatatac catttcctgg ctcacctgct cctaaacgat aattaatccc
     1081 acttgacgtt ttataaacga tcataaagaa tccatctttg gaactggcta tattaggtga
     1141 atagaaggat tcagcaatat ataaattaca cataccgtcg taagcataat tgttagcata
     1201 cgagtaatat gcaaaacgat aaacttggat ataattcccc tctttgaaag aaatataaac
     1261 ccggtattca ttccaatcgt caatattata acagtccata gccggcgaac taccgccgtt
     1321 gacatccaga taaggatgac cattattacc aggcaccttt attagacgcc aatcaccatc
     1381 cggcggtttg gcttcgtacc agatatgtcc ctcactctca taaaccatat gtagccagcc
     1441 atcttgagta cg
//
LOCUS       DAOOMU010000217         1439 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_117797_length_1439_cov_19.080925, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000217 DAOOMU010000000
VERSION     DAOOMU010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117797_length_1439_cov_19.080925"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="PLI55_10305"
     CDS             complement(<1..583)
                     /locus_tag="PLI55_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HPB01263.1"
                     /translation="MSGKIKLDDKYLEKSLRLLERATTLLEKYNIPYWLEGGTLLGII
                     RENRLLPWDNDLDISIRSEDFDRLRRILPKFYYRGMIAKVREHKIDDPPFQKGEVRLI
                     KVYATKYLFFKTPLVLDIFVKKKLDDQYYWVVGVKRRAKKAVPARFYDELTTVQFNNK
                     TYSIPKLTDEYLTYRYGDWRTPVKTWNYIKDDQS"
     gene            complement(596..1324)
                     /locus_tag="PLI55_10310"
     CDS             complement(596..1324)
                     /locus_tag="PLI55_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HPB01264.1"
                     /translation="MKVIILAAGNAIRLASLANGSPKCLLPLGNTTILGKQLHNLEKV
                     GLTDVILSVGYKVEKVEEYLKNFRTNLKIHLEFNPFYDISNNIVSLWNVRHLVWNQPI
                     IIINGDNVFDYQILSLLLESPFENVLMIQEKDEYDGDDMKVKTENDRLLAVNKTMKSS
                     EANGESIGIMKFSAEGCAQLMQKIVEMTRNKENLQVWYLRSIQELINDGLPVHVCRIN
                     GLKWEEVDFPEDYLRVKNMNWEGN"
BASE COUNT          413 a          306 c          237 g          483 t
ORIGIN      
        1 ttgattgatc atcctttata taattccagg ttttgaccgg agtgcgccaa tcaccataac
       61 gataagtcag atattcatca gtcaatttcg gaattgaata agttttattg ttaaactgaa
      121 cggtcgtcag ttcatcataa aaacgagccg gaaccgcttt ctttgcccgc cgctttactc
      181 caaccaccca atagtattga tcatctaatt ttttcttaac gaagatgtcc agaacgagcg
      241 gagtcttgaa aaatagatat ttggtggcat aaacctttat tagccggacc tctccttttt
      301 ggaacggggg atcatcgatt ttgtgctctc tgactttagc gatcattccc cggtagtaga
      361 acttaggcag aatacgccgt aaacgatcaa agtcttcgga acggatggaa atatccaggt
      421 cgttatccca gggaagcagt ctattttctc tgataattcc taaaagagtc ccaccttcca
      481 gccagtatgg aatattatat ttctccagaa gtgttgtggc gcgctcaagc agtcttagcg
      541 atttttccag atatttgtca tccaatttta ttttacctga catatttttt cccgatcaat
      601 taccttccca attcatattt tttacgcgca aataatcttc cgggaagtcc acttcttccc
      661 atttcaatcc gtttattcgg caaacgtgaa ccggtaagcc atcatttatc aattcctgga
      721 ttgatcgtaa ataccaaacc tgcaaatttt ccttattgcg agtcatttcc actatttttt
      781 gcatcagttg tgcacaacct tcggccgaaa atttcattat tccgatagat tcaccgttgg
      841 cttctgaact tttcatagtt ttattaacag ccagaagccg atcattttcg gttttgacct
      901 tcatatcatc accgtcatat tcatcttttt cttggatcat cagaacattt tcaaaaggtg
      961 attccagtaa aagcgaaagt atttgataat caaatacatt atcgccatta atgataataa
     1021 ttggctgatt ccataccaag tgacggacgt tccagagtga aacgatatta ttggaaatgt
     1081 cataaaacgg attgaattcg agatggattt tcaaattagt ccgaaagttt ttcagatact
     1141 cttcgacttt ttcgacttta taaccaaccg agagaatcac atctgttaac ccgacttttt
     1201 ccagattgtg taattgttta cctaaaattg tcgtattgcc aagcggcaaa agacacttag
     1261 gacttccatt agcgagagaa gctaaccgaa tggcatttcc cgccgctaat attattactt
     1321 tcataaatac tcctgattca tattattaat taaatttaat ttactctact taatattgaa
     1381 ttccgaattc tttcagaggc tctgccatct aatttataaa gcatatctgc gatgtattt
//
LOCUS       DAOOMU010000218         1410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_121663_length_1410_cov_11.721033, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000218 DAOOMU010000000
VERSION     DAOOMU010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121663_length_1410_cov_11.721033"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..>1410
                     /locus_tag="PLI55_10315"
     CDS             49..>1410
                     /locus_tag="PLI55_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01265.1"
                     /translation="MKTVKLFILSLLALTFLTCERQKANDEPVFIFQSPKVLFKNLIA
                     NNLIVPRFSPDGLDLIFNGRIDGDLWDGIYQIPIVGGLPQKIYSTTDDILYPSYSADQ
                     RQVIFSQGLARQIYLLDIVTKNVTPLPIYGNYPTILPDGSTILYTGGLDGNIRLYDLR
                     ENRHRVLTKSHSGINIFPMMTQDRVGFIWLENRKPEQVRVNQTDFDALKTKILQIFAE
                     PLLSWTISPSGEWAIASRPNGEPFGFKLYDTAFASISIQPDTLVKNVKHLAFSVDWSP
                     TGRNVVYVGNTVPQFSRENPFTYRGIFKGDLVIANLRWENIEEAELLKSPAVSNWTIF
                     PYSEPNIQGSPRFVPTNINNPPVITSVPVETARQGELYFYRVQAVEIDLFDKITYSVV
                     SGPENATILKNTGILVWQPIDSGRFEFTVAVQDNYDGIDSQTFYVDVLPAKKGDDIFY
                     QSQP"
BASE COUNT          384 a          317 c          302 g          407 t
ORIGIN      
        1 actcttctga ggtgaataga atagtatgcg ctccaacaag atgagaatat gaaaacagta
       61 aaactcttta tcttaagttt attggcgttg acctttttga cctgcgaacg ccaaaaggca
      121 aatgacgagc cggttttcat ttttcagagt cctaaagtgc tctttaaaaa cctgattgcc
      181 aataacctga tcgtgccacg cttttcccct gatggattag acctaatttt caatgggcga
      241 atagatggcg atctctggga cggaatttat cagattccaa ttgtgggtgg tttgccccaa
      301 aagatttata gcaccactga cgatatactt tacccttcat attcagccga tcaacgccag
      361 gtaattttta gtcaggggct tgcccgccaa atctacctgc tggatattgt tacgaaaaat
      421 gttacgccgc ttccgattta tggcaattat cccacaattc tccctgatgg ctctaccata
      481 ctttacacgg gtggattgga tggtaatatt cgtttgtatg atttaagaga aaaccgacat
      541 cgtgttctga caaaaagcca ttccgggatt aatatatttc cgatgatgac ccaagatcgg
      601 gtgggcttta tctggcttga gaatcgaaaa cccgagcagg ttcgtgtcaa ccagactgat
      661 ttcgatgcgc taaagaccaa aatattgcaa atatttgccg agccgcttct gagctggacg
      721 atttcaccaa gtggcgaatg ggcaatcgcc agccgtccaa acggtgaacc tttcgggttt
      781 aaattatatg acacggcttt tgcatcaatt tccatccaac ctgatacatt agtcaaaaat
      841 gtaaaacact tagccttttc ggtggactgg tcaccaaccg gccggaatgt tgtttacgtt
      901 ggcaacaccg tgccgcaatt ttctcgggaa aatcctttta cctatcgcgg aatattcaag
      961 ggcgaccttg tcatagctaa cctgcgttgg gagaacatcg aggaggctga attactaaaa
     1021 tcgcccgcgg tttcaaattg gacaattttt ccctattctg aaccaaatat tcaaggatcg
     1081 ccccgttttg tgccgactaa tatcaataat ccgccggtaa ttacgagcgt cccagtcgaa
     1141 accgctcggc aaggcgaact ttatttctat cgagtacaag ccgttgaaat tgatctgttc
     1201 gataaaataa cctattctgt ggtgtccggt ccggaaaatg ccacaatcct gaaaaatacc
     1261 ggtattttgg tctggcaacc gatcgattcc ggtcggtttg agttcacggt agctgttcag
     1321 gataattatg atggcattga ttcacagact ttttacgttg acgttttgcc tgccaagaaa
     1381 ggggatgata ttttctatca atcccagcct
//
LOCUS       DAOOMU010000219         1402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_122793_length_1402_cov_11.990349, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000219 DAOOMU010000000
VERSION     DAOOMU010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122793_length_1402_cov_11.990349"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..>1402
                     /locus_tag="PLI55_10320"
     CDS             316..>1402
                     /locus_tag="PLI55_10320"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HPB01266.1"
                     /translation="MPYKTVFDAYHLYHLPQFDPVIDLLLNDNRFEVFLTTSSANLPD
                     EQNLTKHIFQRRNMQCIFANNEEERAAKIRSLNPDVFICGWSRYPIEKFVTEKTLVGM
                     IYHGIGVKPSYWRDNHPRLNIRFVEGPFRMRQLREKGIQTDLELTGFPKIDPLFNGQI
                     ASREEILRNLGLDFNKKTLLYAPTFYPSSFESFGMKLPEMAKDLNLIIKLHQWSYFLE
                     KFSGVNLRRHIKLANKIRKKYPRVVMIEPEHYNIIDLYQASDILLTEASSTIYEFMAL
                     RKHIIICDFFKKKIEHYLLPKRIYLHRLDPEMHGILTEFCYHIKKPQDLPVALDKCFQ
                     QPDPFVELREKYIADMLYKLDGRASERI"
BASE COUNT          427 a          264 c          271 g          440 t
ORIGIN      
        1 ccatccgccg atcatatctg ggacacactt ggccaattgt tatttgcggc tgggcgggtt
       61 gatgaagcta tccaggctat gcaaaaagcc agcgaactca atccgcagga aaagtcatat
      121 caggaactat tgataaagta caaaaaggcg ctgaatagtt gattggtcta tattttggat
      181 tttgacaatt attgaagaca ataaaatatt ttgcgcatta ttattttaaa agtaagtctg
      241 ttttggaagt ttttttgccg attcttttgg gcgattttaa tttgtctacc gtattaatag
      301 agttttagaa aatctatgcc atataaaact gttttcgatg cctaccattt atatcatctc
      361 ccgcaattcg atccggttat tgacctcttg ttaaatgata atcgctttga ggtttttctg
      421 acgacctcaa gtgctaattt gccagatgaa cagaacctga cgaaacatat atttcagcgg
      481 cgcaatatgc agtgcatttt cgctaataat gaagaagaac gcgccgctaa gattcgatct
      541 cttaatccgg atgtattcat ctgtggttgg tcgcgttatc caatcgagaa attcgttaca
      601 gaaaaaactc tggtcggtat gatctatcac ggaattggag ttaaaccatc ctattggcga
      661 gataaccatc cacgattgaa tatccgcttt gtcgagggtc cctttcgtat gcgacaactg
      721 cgtgaaaaag gaatacagac tgatttggaa ctgacaggct ttcccaaaat tgatccgctg
      781 ttcaatggcc agattgccag ccgggaggaa attttacgta atcttgggct ggactttaat
      841 aagaaaaccc tgttatatgc tccgactttt tatcccagta gttttgaatc atttgggatg
      901 aaattgccag aaatggctaa agatttgaac ttgataatca aattgcatca atggtcttat
      961 tttttggaaa aatttagtgg ggtcaatcta cgccgtcata ttaaactagc taacaaaatc
     1021 aggaagaaat atccccgagt ggtgatgatc gagccagaac actataatat tattgacctt
     1081 tatcaggcat ctgatatttt attaacggaa gcctcttcga ctatttatga gtttatggct
     1141 ttacgcaaac atataatcat ttgtgatttt ttcaagaaaa agattgaaca ctatctctta
     1201 ccaaaacgaa tttatttgca tcgtcttgat cccgaaatgc acggaattct gaccgaattt
     1261 tgctatcata tcaaaaaacc gcaagattta cctgtagctc ttgataaatg tttccaacaa
     1321 cctgacccgt tcgtcgaatt acgtgaaaaa tacatcgcag atatgcttta taaattagat
     1381 ggcagagcct ctgaaagaat tc
//
LOCUS       DAOOMU010000220         1374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_126756_length_1374_cov_16.633813, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000220 DAOOMU010000000
VERSION     DAOOMU010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126756_length_1374_cov_16.633813"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLI55_10325"
     CDS             <1..452
                     /locus_tag="PLI55_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007555735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPB01267.1"
                     /translation="LAAGRTGKVVNVKKGQFLAPQDVVNIVRKIESTGNHSILLTERG
                     VSFGYGGLITDVRAVPIMQETGYPVIFDATHSAQIPGGNVTGGAREYIPHLSRAMVAA
                     GCDGIFMEVHDNVAEAKSDRATQYPLEDLPKLLKELIELGELVRKFQ"
     gene            449..997
                     /locus_tag="PLI55_10330"
     CDS             449..997
                     /locus_tag="PLI55_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010946575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPB01268.1"
                     /translation="MNTDINELLSKIRLIIADVDGVFTDGALYIGTDGQEFKRFHVLD
                     GAGISLLKAVNFPLAIISGRKSAATEVRMQELGLGDALYQGNLAKLEPYAEIKKRYAV
                     ADQEIIYIGDDLPDIPLMKRVGLPVAVANALPEVKQFARYITVLGGGYGAVREVIDLV
                     LNAQHLKSKAIGILTGDTYAEK"
     gene            984..>1374
                     /locus_tag="PLI55_10335"
     CDS             984..>1374
                     /locus_tag="PLI55_10335"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPB01269.1"
                     /translation="MPKNKSTSEKYLEKARELLRSEAEAIATLPDKVDQNFKEVVEVL
                     FNTKGKVIVTGVGKSGHIGCKIAATFASTGTPSFFMHAYEAGHGDLGVISPDDTLLII
                     SNSGETDEIIQLIPPLKKMRIPIIGLLG"
BASE COUNT          387 a          296 c          350 g          341 t
ORIGIN      
        1 tactggcggc gggtcgaacc ggaaaagtgg ttaatgtgaa aaaaggacag ttcttggcgc
       61 ctcaggatgt cgtcaatatt gtgcgcaaaa tcgaatcaac cggcaatcat tcaattttgc
      121 tcaccgagcg gggagtctcc ttcggatatg gcggattaat taccgacgtc cgcgcggtgc
      181 cgattatgca agaaaccggc tacccggtaa ttttcgatgc cacccacagc gcccaaattc
      241 ccggcggtaa tgttaccggc ggagcgcggg aatatattcc ccatctcagt cgcgctatgg
      301 tggcggctgg atgcgacggc atttttatgg aagtacacga taatgtggcc gaagccaaga
      361 gcgaccgggc gactcaatat ccgctggaag atttaccaaa attattgaaa gaattgatag
      421 agctgggcga gttagtgaga aaatttcaat gaataccgac ataaatgaac ttctttcgaa
      481 aatccgcttg attattgcgg acgttgatgg agttttcacc gatggggcgc tatatatcgg
      541 cactgacggt caggaattca aacgctttca tgttttagac ggagcaggaa tttcgttgct
      601 gaaagctgta aactttccgc tggcaattat ttcggggcgg aaatctgcgg ctacagaagt
      661 gcggatgcaa gaactcggtc tgggggatgc gctttatcag ggaaatctag ccaagctcga
      721 accctatgca gaaattaaga aaaggtatgc cgtcgcggat caggaaatca tttatatcgg
      781 tgacgacctc cccgatattc ctctgatgaa gcgagttgga ttgccagtag cggtagccaa
      841 tgccttacca gaagtcaagc aatttgccag gtacattacc gtgttaggcg gcggatatgg
      901 tgctgtgcgc gaagtgattg atttagtgct aaatgcccag cacttaaagt caaaagcgat
      961 tggaatttta acgggggata cctatgccga aaaataaatc aacttcagaa aaatatctcg
     1021 aaaaagcccg ggaattactc cgttccgaag ccgaagctat tgctacccta cccgataaag
     1081 ttgaccagaa ttttaaagaa gtggtcgaag ttttattcaa caccaaaggc aaagtaatcg
     1141 taaccggagt cggcaaatcc ggacatattg gatgcaaaat agccgctact tttgccagca
     1201 caggtacgcc gagttttttt atgcatgctt atgaagcggg acatggcgac ctgggggtaa
     1261 tttctcctga tgatactctt ttgattattt cgaatagcgg tgaaaccgac gaaatcattc
     1321 aattaattcc accgctgaaa aaaatgcgca ttcccatcat tggcttattg gggc
//
LOCUS       DAOOMU010000221         1374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_126758_length_1374_cov_15.065959, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000221 DAOOMU010000000
VERSION     DAOOMU010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126758_length_1374_cov_15.065959"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLI55_10340"
     CDS             complement(<1..391)
                     /locus_tag="PLI55_10340"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPB01270.1"
                     /translation="MPKNKSTSEKYLEKARELLRSEAEAIATLPDKVDQNFKEIVEVL
                     FNTKGKVIVTGVGKSGHIGCKIAATFASTGTPSFFMHAYEAGHGDLGVISPQDTLLII
                     SNSGETDEIIQLIPPLKKMRIPIIGLLG"
     gene            complement(378..926)
                     /locus_tag="PLI55_10345"
     CDS             complement(378..926)
                     /locus_tag="PLI55_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HPB01271.1"
                     /translation="MNADLNELLSKIRLIIADVDGVFTDGALYIGTDGQEFKRFHVLD
                     GAGISLLKAVNFPLAIISGRKSAATEVRMQELGLGDALYQGNLAKLEPYAEIKKRYAV
                     ADQEIIYIGDDLPDIPLMKRVGLPVAVANALPEVKQFARYITVLGGGYGAVREVIDLV
                     LNAQHLKSKAIGILTGDTYAEK"
     gene            complement(923..>1374)
                     /locus_tag="PLI55_10350"
     CDS             complement(923..>1374)
                     /locus_tag="PLI55_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPB01272.1"
                     /translation="LAAGRTGKVVNVKKGQFLAPQDVVNIVRKIESTGNRSILLTERG
                     ISFGYGGLIADVRSVPIMQETGYPVIFDATHSTQIPGGSVTGGAREFIPHLSRAMVVA
                     GCDGIFMEVHDNVAEAKSDRATQYPLEDLPKLLKELIELGELVRKFQ"
BASE COUNT          357 a          345 c          284 g          388 t
ORIGIN      
        1 gccccaataa gccaatgatg ggaatgcgca tttttttcag cggtggaatt aattgtatga
       61 tttcatctgt ttcgccgcta ttcgaaataa tcaaaagagt atcctgagga gaaataactc
      121 ccaggtcgcc atgtcccgct tcataagcat gcataaaaaa actcggcgta ccggtactgg
      181 caaaagtagc ggctattttg catccaatat gtccggattt gccgaccccg gttacgatca
      241 ccttaccctt ggtattgaac agaacttcga caatttcttt aaaattctgg tcaactttgt
      301 caggtaaggt ggcaatggct tcggcttcag aacggagtaa ttcccgggct ttttcgagat
      361 atttttctga agttgattta tttttcggca taggtatccc ccgttaaaat tccaatcgct
      421 tttgacttta agtgctgggc atttagcact aaatcaatca cttcgcgcac agcaccatat
      481 ccgccgccta acacggtaat gtacctggca aattgcttga cttctggtaa ggcattggct
      541 accgctactg gcaatccaac tcgcttcatc agaggaatat cggggaggtc gtcaccgata
      601 taaatgattt cctgatccgc gacggcatac cttttcttaa tttctgcata gggttcgagc
      661 ttggctagat ttccctgata aagcgcatcc cccagaccga gttcttgcat ccgcacttct
      721 gtagccgcag atttccgccc cgaaataatt gccagcggaa agtttacagc tttcagcaac
      781 gaaattcctg ctccgtctaa aacatgaaag cgtttgaatt cctgaccgtc agtgccgata
      841 tatagcgccc catcggtgaa aactccatca acgtccgcaa taatcaagcg gattttcgaa
      901 agaagttcat ttaagtcggc attcattgga attttctcac taattcgccc agttctatca
      961 attctttcaa taattttggt aaatcttcca gcggatattg ggttgctcgg tcactcttgg
     1021 cttcggctac attatcgtgc acttccataa aaataccatc gcatccagcc accaccatag
     1081 cccgactgag atggggaata aattcgcgtg ctccaccagt aacactgccg ccgggaattt
     1141 gggtactgtg agtagcatcg aaaattaccg ggtagccggt ttcttgcata atcggcaccg
     1201 agcggacgtc tgcaataagt ccgccatatc cgaaagaaat tcctcgctcg gtgagcaaaa
     1261 tcgaacgatt accggttgat tcaattttgc gcacaatatt gacaacatct tgaggtgcta
     1321 agaactgtcc ttttttcaca ttaaccactt ttccggttcg acccgccgcc agta
//
LOCUS       DAOOMU010000222         1354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_129704_length_1354_cov_4.880677, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000222 DAOOMU010000000
VERSION     DAOOMU010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129704_length_1354_cov_4.880677"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..>1354)
                     /locus_tag="PLI55_10355"
     CDS             complement(240..>1354)
                     /locus_tag="PLI55_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HPB01273.1"
                     /translation="PGLLSAMDVVNEAVKDDGTDRTESEWYITFGDSSYIMKAFEFTR
                     KWTEYYGETQMKLYYNDYNTHIPAKADGIVRICEPIYEAGYLDGIGMQDHDQYNSPTA
                     EQWIASYEKFEPICDEIAVTELDVKPSTDSATRWTTQANQYGMLFKCFVERSFFSGRG
                     KIISVSKDGLNDEWAFVKNASLWDSQNQCKPAFYEVVKVGINYNALDSLFSITDTLSE
                     NKYTTESWELLMASLSKAQYVMNRNYSYTVSAGDSLENAKNNLDLAIRSLVVSSSNVN
                     EYENTIPKQFVLNQNYPNPFNPTTTIGYQLPISGYLTLKVYNLLGQEVATIFEGVQSA
                     GNYSVTFDANGLTGGIYFYQLKAGSFVENKKFILLK"
BASE COUNT          383 a          274 c          254 g          443 t
ORIGIN      
        1 ggtaactcat aggtttggtg aatcggtcca tacggaccac cggatggctg agccgttgct
       61 atcagaaatg aacttaagta aataaacaaa ctaccgatgg caaatcgttt gtttgttctc
      121 ggtaatgaat ttccgcttac tggtttgaat cgcattttca tttttaattt cctttcttca
      181 gttatcaaat atcttggaat aaatatatca ttatttttta tgcctgtttt aaatatattt
      241 tatttcagta gaatgaattt tttattttct acaaaactcc cggctttcaa ttgataaaaa
      301 taaatcccgc cagtcaatcc attggcatca aaggttaccg agtaatttcc ggctgattga
      361 acaccttcga atatcgtggc gacctcctgc ccgagcagat tatagacttt gagagttaaa
      421 taaccggata ttggcagttg gtaaccgatg gttgtagtcg gattgaaggg attgggataa
      481 ttctggttga gcacgaattg tttgggaatc gtattttcat attcgttaac attactcgaa
      541 ctaaccacta aacttctaat tgccaaatca agattatttt tagcattttc taaactatct
      601 ccggcagaaa ctgtatagga ataatttcta ttcataacat attgggcttt cgataatgat
      661 gccattagca gttcccatga ttcagtggta tatttatttt cactcagcgt atcagtgata
      721 gagaagagtg aatcaagtgc gttgtaattt atcccgactt ttacgacttc atagaaggct
      781 ggtttgcatt gattttggga atcccagagg gaagcgtttt ttacaaacgc ccattcatcg
      841 ttcagaccat ctttagatac actaataatt ttaccccgac ccgagaagaa gctcctttct
      901 acaaagcatt taaataacat accgtactga ttcgcttggg tcgtccagcg ggttgcggaa
      961 tcggtgctgg gtttaacgtc tagctcggta actgcaattt catcgcaaat cggttcaaat
     1021 ttttcatagg aagcaatcca ctgctcagcg gtgggtgaat tgtattggtc gtgatcttgc
     1081 ataccgatcc catccagata tccggcttca taaatcggtt cgcaaattct cacaatgcca
     1141 tcagcttttg cagggatatg ggtattataa tcattatagt aaagcttcat ctgagtttca
     1201 ccgtaatatt cagtccattt acgagtgaat tcaaaagcct tcattatata actactatca
     1261 ccaaaagtaa tgtaccattc actctcggtt cgatcagtgc catcatcttt gacggcttca
     1321 tttaccacat ccatcgccga aagcaagcca ggcc
//
LOCUS       DAOOMU010000223         1303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_137715_length_1303_cov_16.260417, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000223 DAOOMU010000000
VERSION     DAOOMU010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137715_length_1303_cov_16.260417"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1124)
                     /locus_tag="PLI55_10360"
     CDS             complement(144..1124)
                     /locus_tag="PLI55_10360"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HPB01274.1"
                     /translation="MRRLIFFLFPIALLLWRCPAGPPTGPSTDISRELCANCIISPFE
                     DTTYVFVSRWSEISEYEFEDIVTPEMIINPYWDYWSISNAKIRIAVDGKNLPVEEDSV
                     RGYKEFYRIYHYYYYKFPNVDVRPGQHWELSVYHPDYDSLYAETVVPDSVEFINLPDT
                     IRDDSENLNFSWTDADGARGYLPLLSFTAINDTGKYWSVQIHADFTDEGDGVYSSSSG
                     YPRQNTSAEYKINTMLKEIKFCYDEDKYKIGDLSQFTSFYLRFYVYSLNEALYNIQRW
                     EVQPDELSGFNAPVRVFSNVENGSGILGAFWRTASKKIIIDKEFLIGQCQ"
     gene            complement(1121..>1303)
                     /locus_tag="PLI55_10365"
     CDS             complement(1121..>1303)
                     /locus_tag="PLI55_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01275.1"
                     /translation="YHRLDINFVKQYSWRSHPMEFYIDLVNVYARLNVLAYAEHNDTW
                     IQMPPLATIGIRGKIW"
BASE COUNT          380 a          282 c          256 g          385 t
ORIGIN      
        1 tcgtaggaca gcctttctag gctgtcagct atatattatc cgtcattaga atagacaggc
       61 aagaatgcct gtcctacgga attttgcatt atggggcggc gtgctaaatc gaatggtcga
      121 attggtgact cgacctacag gttttattga cattgaccaa ttaaaaattc tttatcaatg
      181 atgattttct tgctggctgt tcgccagaag gcgcccagga tgccgctgcc gttttcgaca
      241 ttggaaaaaa cgcgaaccgg ggcattgaaa ccgctgagtt catcgggctg aacttcccag
      301 cgctggatat tataaagggc ttcgttcaat gagtaaacat aaaatcgcaa ataaaaagaa
      361 gtgaactggc tcagatcacc aattttatat ttatcttcat cataacaaaa tttaatctct
      421 tttaacatag tatttatttt atactcggca gatgtatttt gacggggata accggaggaa
      481 gatgagtata ctccatcgcc ttcatcagta aaatctgcgt gtatttgaac actccaatat
      541 ttacctgtat catttatcgc cgtaaaagat aataatggta aatatcccct ggcgccatcc
      601 gcatcagtcc atgagaagtt taagttctct gaatcgtccc taattgtatc tggcaagttg
      661 ataaactcaa cggaatccgg caccaccgtt tcggcataga gactatcgta atccggatga
      721 tataccgata attcccaatg ctgtccggga cgcacatcca catttggaaa tttgtagtag
      781 tagtaatgat agattctata gaattcttta tatcccctaa ctgagtcttc ttcaacagga
      841 agattttttc catccaccgc aattcttatt tttgcgttag aaatgctcca atagtcccaa
      901 tagggattta ttatcatttc cggagtcact atatcttcaa actcgtactc acttatttca
      961 ctccatctcg agacaaaaac gtaagtagtg tcttcgaatg gactgataat acaatttgca
     1021 cataattccc ggctgatatc ggtactgggt ccggtcggcg gtccggcggg acaacgccat
     1081 aataaaagtg cgattggaaa taaaaagaat attaatcgtc tcaccatatt ttccccctta
     1141 tcccgatcgt tgccagcggc ggcatctgta tccaggtatc attatgttcg gcataagcca
     1201 ggacattcag acgagcatag acattgacca aatcaatata aaattccatc ggatgcgatc
     1261 tccagctgta ttgtttgacg aagttaatat ccagccggtg ata
//
LOCUS       DAOOMU010000224         1299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_138457_length_1299_cov_3.751608, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000224 DAOOMU010000000
VERSION     DAOOMU010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138457_length_1299_cov_3.751608"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1299)
                     /locus_tag="PLI55_10370"
     CDS             complement(<1..>1299)
                     /locus_tag="PLI55_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPB01276.1"
                     /translation="DFAFIDEENIITNYGGSVFLDYKLPNGKIILQNALTHTLNDNSK
                     YRLEYDLSINEIGYGLNRDKHNKELLINALQANYNFGLVQAELGLSHSFSDKNTDIRY
                     GDPGNEFGFLNAKSDVPFVDDNGNPIYYDVARRFFTPEDVYNIKVNENDWENAEIYGW
                     FVTRDEAFKQHLYNGTLDFTIPVSFLSVVSGDIKLGGKYTSSTRTNDVEESYRRTGAA
                     DFYIGIDSTWLGEKFNPATNPITFANLRNNDYESERGKYFFESDYPFQYALDKELTDE
                     FIEKAIYGWNHPSEFDPTVTKPINYRHKAKSLRDDFDGSEVFSAAYLMGDFNIGPRLS
                     LIGGGRFEHFNMDYDANFVYVRHPVDGDANDTTRSVDRNDDTFFPNAQLRYKFTEWGD
                     IRAAYSKSIARPDFLAILPNVYYPKSEDALAGNPGLKPSIS"
BASE COUNT          405 a          269 c          231 g          394 t
ORIGIN      
        1 tgatattgat ggttttaatc ccggattacc agccagagca tcttcggatt taggataata
       61 gacatttggt aaaatagcca ggaaatctgg acgcgctatt gatttggaat aggccgcccg
      121 gatatcaccc cattcggtaa atttatagcg caattgagcg ttggggaaaa aggtatcgtc
      181 attacgatca actgatcggg tggtatcatt ggcatcacca tctaccgggt gtctcacata
      241 aacaaaattg gcatcataat ccatattgaa gtgctcgaat cttccgccac caattaatga
      301 aagacgcggc ccaatattga aatcacccat tagatatgcg gctgaaaaca cttcggaacc
      361 gtcgaagtca tctctcagcg acttagcttt atgtctataa tttataggct tagttacagt
      421 cgggtcaaat tcagagggat gattccaacc atatatcgcc ttttcaataa attcatccgt
      481 cagttcttta tccagagcat attggaaggg atagtcactc tcaaaaaagt attttcctct
      541 ttcactctca taatcattat ttctcaaatt ggcaaaggta atcggattgg ttgccggatt
      601 gaatttttcc cctagccagg tactatctat tccgatataa aaatccgcgg caccggttct
      661 acgataagat tcctcgacat cattcgttcg ggttgaacta gtatatttac cccctagttt
      721 aatatcaccc gaaacaacgc tcaaaaaaga gaccggaata gtaaaatcca gagtgccatt
      781 atataaatgt tgtttaaatg cttcgtcgcg agtcacaaac cagccgtaga tttcggcatt
      841 ttcccagtca ttttcattaa cttttatatt atatacatcc tcaggtgtaa aaaaacgccg
      901 cgccacatcg taatagatgg gattaccatt atcatcaaca aaaggaacat cagatttggc
      961 attcaggaag ccaaactcgt tacccggatc gccgtatctt atatcagtgt ttttatcact
     1021 gaaggaatgc gataaaccta attcggcctg gaccagacca aaattataat tagcttggag
     1081 agcattaata agcaactctt tattatgttt atcacgattt agtccatatc caatttcatt
     1141 aatactgaga tcatattcca gacgatattt tgaattatcg tttaacgtat gggtaagtgc
     1201 attttgtaat ataattttac cattgggaag tttataatct agaaaaacac tcccgccata
     1261 gttagtaatg atattttctt catcaataaa agcaaaatc
//
LOCUS       DAOOMU010000225         1285 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_140878_length_1285_cov_5.212195, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000225 DAOOMU010000000
VERSION     DAOOMU010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140878_length_1285_cov_5.212195"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PLI55_10375"
     CDS             complement(<1..1283)
                     /locus_tag="PLI55_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPB01277.1"
                     /translation="MSGALTPVQNLRAENDIQPRQRLSLNDDWKFYKYESPNDADDII
                     YDVRPQINDYNDVKPADTRPTESEEIQATRRVLKPWILPNGNDFIKDSTKQCRRPEGN
                     PGRDFPFVQKDYDDSRWESVNLPHDWAIKGPFFEGPNPEVGGGMGRLPSPGVAWYRKK
                     LNIPATDAGKSLFLDIDGAMSYAMVWLNGYLVGGWPYGYSSWRLDLTPYIIPGAENQL
                     AIRLDNPNHSSRWYPGGGIYRNVWLVKTNPVHIAQWGTYITAENVSVTSATLNLEITI
                     DNDSRNDANVKAITEVFILNENGDKTGEAVASFQPLEILISAGARTSEKSSIIIENPR
                     LWGPPPTQTPNCYIAVTTLWQGNQLIDRYETRFGIRSLRFEPDSGIYVNGELIKIKGV
                     NQHHDLGALGAAFNRRAAERQLEILREMGCNAIRTA"
BASE COUNT          325 a          321 c          309 g          330 t
ORIGIN      
        1 gcagtgcgaa tcgcattaca acccatttcc ctcaaaatct cgagctggcg ctcagccgcg
       61 cggcgattga aagccgctcc caaagcgccg aggtcatggt gctgattgac acccttaatc
      121 ttgatgagtt cgccattaac atatatcccg gaatccggct cgaaacgcaa agaacgaata
      181 ccgaaacgcg tttcgtagcg atcaattagt tggttaccct gccacagagt agtaaccgca
      241 atgtaacaat tgggtgtttg agtaggcggc gggccccaca accttggatt ttcgataata
      301 attgaacttt tttcgctggt tcgagcaccg gcggaaatta aaatttccag cggttggaaa
      361 ctggcgacag cctcaccggt tttatcgcca ttttcattta aaataaaaac ctcggtaatc
      421 gcctttacat tggcatcatt ccgggaatcg ttatctatgg taatttctag gtttagcgta
      481 gcggaggtca ccgaaacatt ttcggccgta atgtaagtcc cccactgcgc gatatgaacc
      541 ggattagttt tgaccagcca gacattgcgg taaattccgc cgccggggta ccagcgcgag
      601 gagtgattgg gattatcaag acgaatcgcc agctgattct cagcacccgg aatgatataa
      661 ggcgtaaggt cgagacgcca ggaagaataa ccgtaaggcc agccgccgac caaataacca
      721 ttcaaccaaa ccatagcgta ggacattgcg ccgtctatat cgagaaatag ggacttgccg
      781 gcgtcggtag ctggaatgtt caattttttc cgataccagg cgacgccggg gctgggaagt
      841 cgccccattc caccgccaac ttccggattt ggtccctcaa aaaacggtcc cttgatcgcc
      901 caatcgtgtg gcagattcac gctctcccac cgactatcat cataatcttt ttgcacaaaa
      961 ggaaaatcgc ggccgggatt accctcggga cgacggcatt gtttcgtaga atcttttata
     1021 aaatcattgc cgttcggtaa aatccagggc tttagcaccc ttcgggttgc ttgtatttct
     1081 tcactttcgg tcggtcgagt atcagccggt ttgacatcgt tataatcgtt aatttgcgga
     1141 cgcacatcgt agattatgtc gtcggcatcg ttcggagatt cgtacttata gaatttccaa
     1201 tcgtcgttta aagaaaggcg ctgacggggc tgaatatcat tttctgccct tagattttgg
     1261 actggagtta gagcgccact cagta
//
LOCUS       DAOOMU010000226         1284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_141031_length_1284_cov_22.139951, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000226 DAOOMU010000000
VERSION     DAOOMU010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141031_length_1284_cov_22.139951"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1116
                     /gene="wecB"
                     /locus_tag="PLI55_10380"
     CDS             1..1116
                     /gene="wecB"
                     /locus_tag="PLI55_10380"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPB01278.1"
                     /translation="MDLIVGARPNYMKAAPVYRALKKHPEKFKVRLVHTGQHYDEKMS
                     HIFFRELELPQPDVYLGVGSGRHGEQTGKIMIEYEKAVLFDKPDLVLVAGDVNSTMAC
                     SLVAAKLHIKVGHIEGGLRSGDWSMPEEINRIVTDRIADYLFTTSKDADENLLAEGIS
                     PDKIFLVGNTMIDSLNYYLPKAQQLDLKSKYSIDLDASSQPIEEHKFILVTLHRPSNV
                     DDGKILQDLIKVLCQIQEEMPVIFPMHPRTRKMLSEFDLNGMLESARNLIITEPLGYL
                     EFLKLEATAFLVLTDSGGIQEETTVLGIPCLTLRENTERPITIWEGTNELVGSNPQKV
                     LERTYAILNGERKTGKVPHFWDGHAAERIVEILLNKR"
BASE COUNT          387 a          267 c          288 g          342 t
ORIGIN      
        1 attgacctta tcgtcggtgc acgaccgaat tatatgaaag ccgctccggt ttaccgagca
       61 cttaaaaaac atcccgaaaa attcaaagtt cgtctggtgc ataccggtca acattatgat
      121 gaaaagatgt cgcacatttt ttttcgggaa ctggaattgc ctcagccgga tgtttacctc
      181 ggggttggtt ctggcaggca tggcgaacaa actggcaaga taatgatcga atacgagaaa
      241 gccgttctat tcgataaacc ggatctggta ttagttgcgg gggatgtcaa ttctactatg
      301 gcttgttcct tagtagccgc taaattgcat attaaagttg gtcatatcga aggcggtctc
      361 cgtagtggtg attggtcaat gccggaagaa atcaatcgca tcgtaaccga ccgaattgcc
      421 gattatcttt ttactaccag taaagatgcc gacgagaact tattggctga gggtatttca
      481 ccggataaaa tttttctggt cggcaacacg atgattgatt ctctgaatta ttatctaccc
      541 aaagcccagc aattggattt gaagagtaaa tattcaattg acctcgatgc gtcaagccag
      601 ccaattgaag aacataaatt tatcttagta accctgcatc ggccttctaa tgttgatgac
      661 gggaaaattt tacaagactt gattaaagtt ctatgccaaa ttcaggaaga aatgccagtc
      721 atttttccga tgcacccacg cacccgcaaa atgctaagtg aatttgattt aaatggaatg
      781 ctggagagcg ccagaaatct aatcatcacc gaaccgctgg gttacctgga attcctgaaa
      841 ttagaggcga ctgcattttt ggtcttgact gattcaggcg ggatacagga agaaactact
      901 gttctgggaa tcccctgttt gactttacga gagaataccg aacgtcccat cacaatctgg
      961 gaaggcacca atgaattggt tggttcgaat ccacaaaaag tattagaacg cacttacgca
     1021 atattaaatg gcgagcgtaa aaccggaaaa gtgccgcatt tttgggatgg gcacgccgct
     1081 gagcgcatcg tagaaatctt gctgaataaa cggtaaagcc ggtttgtcag ttcttttttc
     1141 ctttgccgaa aaaagaacca aaaaaggcaa cggctaaaga acatctcacg ggggttcgct
     1201 cagtcccagc ctaaaagaaa ggttaaggct gagaaaaaag gctaagattt aagtacttct
     1261 cagcctcaat ctctttcctt aaaa
//
LOCUS       DAOOMU010000227         1267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_144035_length_1267_cov_17.627063, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000227 DAOOMU010000000
VERSION     DAOOMU010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144035_length_1267_cov_17.627063"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..455)
                     /locus_tag="PLI55_10385"
     CDS             complement(105..455)
                     /locus_tag="PLI55_10385"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPB01279.1"
                     /translation="MSSLIMLKSKIHNARVTATNLEYEGSIEIDAELIRLANLHVFEK
                     VLVLNLNSGVRFETYVIEGKAGQRDISLNGAAARLVEKGDRIIILAFAVVSPAELGDW
                     HPTIIRLDANNNPL"
     gene            667..1209
                     /locus_tag="PLI55_10390"
     CDS             667..1209
                     /locus_tag="PLI55_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPB01280.1"
                     /translation="MFRLINQKLSNPVFDWFMPFISSKSFWLIPIFIGLVLLFWLGKK
                     RGRIATLLIILTIATADPFCARVLKPTFKRLRPSHELSDVRLLGKKGGKYGFPSNHAA
                     NVAGSMLILAFFYRRYKYLCGGLALVVGYSRIYLGVHYPTDVLAGFVIGIGISVGWIL
                     IWRSLTNHLLKKRNDLLALS"
BASE COUNT          350 a          260 c          237 g          420 t
ORIGIN      
        1 tcattttcaa agtgcctgac tcagccacaa tctatgcggt tggacaggca ttttttattt
       61 ttttaaaagg atttttggta cagtacgcca gattcgatga gaaatcaaag cggattgtta
      121 ttggcatcga gcctaattat cgtgggatgc caatcgccta attcagccgg tgatacaacc
      181 gcaaaagcca gaataataat gcgatcacct ttctcgacca agcgagcggc agcaccattt
      241 aaactaatgt ctcgctgacc ggctttgccc tcaatcacgt aagtttcgaa gcgaactccc
      301 gaatttaaat tcaaaaccag cactttttcg aaaacatgca aatttgccaa gcgaatcaat
      361 tccgcatcaa tttcgatact accctcatat tccaagttcg tggcagtaac tcgggcattg
      421 tgtattttag attttagcat tatcaaacta ctcataattc attcctataa tcaaactcat
      481 ttccataacg aatttaccaa ttaaagttac tttattcaaa tttcatgttt tcttacttgg
      541 ttgttcaaat ataaatttga gtggttttaa tatcctattt tgttaccatt ttaattgacg
      601 tttaatgaat ttttaatgaa ggtttagatt tacttgccga gtttgattta ccagttggat
      661 cttaaattgt ttcgtctcat caatcagaaa ctgagcaatc ccgtattcga ttggtttatg
      721 ccctttatca gcagtaaatc tttctggttg attccaattt tcatcggatt agttctcctt
      781 ttctggctgg ggaaaaaaag aggacggatc gccacacttt taattatttt aactattgct
      841 accgcggatc cgttctgcgc ccgagtatta aaaccgacct tcaaacgctt acgtccctcg
      901 cacgaactca gcgacgtccg cttattggga aaaaagggcg gcaaatacgg gtttccatcc
      961 aatcatgccg ccaatgttgc cggttcaatg ctaattctgg cttttttcta tcgccgatat
     1021 aaatatcttt gcggcggatt ggcacttgta gtcggttaca gccgaattta cctgggagtg
     1081 cattatccga cggatgtgct ggcgggattc gtgatcggga ttggtatatc ggtaggttgg
     1141 attctcattt ggcgctcctt aaccaaccat ttattgaaaa aaagaaacga tttattggct
     1201 ttaagttagg attttgaatt ggcataagca gtatataaaa attttagaat tttcgaattc
     1261 ctatcat
//
LOCUS       DAOOMU010000228         1251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_146988_length_1251_cov_13.081940, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000228 DAOOMU010000000
VERSION     DAOOMU010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146988_length_1251_cov_13.081940"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1070
                     /locus_tag="PLI55_10395"
     CDS             333..1070
                     /locus_tag="PLI55_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HPB01281.1"
                     /translation="MKAVILAAGAAHRLAPMTNSIPKCLIKIGDKSILENQLDALQFY
                     GIEDVLIVVGYLKEQIIEKIGNQYKTINISYIENPLFSSTNTVYSLWLARAYFAGQDF
                     FYFNADVLFHYSLIGKLLEAPYESAMGVEVKQCGEEEVKVIVDDKLRIRRIGKKLNPA
                     DCLGEFVGVAKFGASLTADFINALKAVIDEGLQMSFFEAAVDRILDQHALYSVDISKI
                     PVIEIDFPEDLERARQQIYPKIISFID"
BASE COUNT          376 a          230 c          269 g          376 t
ORIGIN      
        1 ggtgattggc gcactccggt caaaacctgg aattatataa aggatgatca atcaatcgta
       61 tcagttagtg aaagaacaac ccaaagaacg actgattaat tttatttcca atacatttct
      121 gaagaaagaa gagagtcttt attaggattt gctgataatt gaagaaattt agacgagatt
      181 gaacctactt agtgagcaac cgatttcatt caaaaagttc ggaataatac aagaaccatc
      241 gactgatttt ggcaatttta gtctttatcg cctaaattaa gcggattttt gtatatagaa
      301 ataggataat aactaaatag gaaaataagt taatgaaagc cgtaatactt gccgccggag
      361 ccgcgcaccg cttagcacct atgaccaact cgattccgaa atgtctgatt aaaattggag
      421 ataagtcaat acttgaaaac cagctggatg ccttgcaatt ctatggaatc gaagatgtct
      481 tgatagtcgt cggttatctc aaagagcaga tcatcgagaa aatcggcaac cagtataaaa
      541 caataaatat ttcctatatc gaaaacccgc ttttttcttc gacgaatacg gtttactcgc
      601 tctggctggc gcgcgcatat tttgctggtc aagatttttt ttatttcaat gctgatgtgc
      661 tttttcacta cagtttaatc gggaagctat tagaagcgcc ttacgaatct gctatgggtg
      721 tggaagtcaa gcaatgcggt gaagaagaag taaaagtcat cgttgacgat aaactgcgta
      781 ttcggcggat cggtaagaaa ctcaatccag ccgattgtct cggtgaattt gtgggcgttg
      841 ccaaatttgg tgcgtctttg acagccgact ttatcaatgc tttgaaggcg gtaattgatg
      901 aaggtctgca gatgtctttt ttcgaagccg ccgtcgatcg gattcttgat cagcacgctt
      961 tgtattcggt tgatataagc aaaataccag tcatcgaaat tgattttccg gaagacctcg
     1021 aacgcgctcg ccagcaaatc tatccgaaaa tcatcagttt tattgattaa ccgtaaaact
     1081 cgtttatcat atttagtgtt tcaaacggaa ggtattataa tagtgagggg taattagtaa
     1141 tgacgatggt cctctttcac cttcatcacc tttattatct gccgcagttt ttacccgtgg
     1201 cgcaggcgat ggagcgtgat ggtgattttg aagtgtttta ctcaacggta a
//
LOCUS       DAOOMU010000229         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_148755_length_1241_cov_12.274874, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000229 DAOOMU010000000
VERSION     DAOOMU010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148755_length_1241_cov_12.274874"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1241
                     /locus_tag="PLI55_10400"
     CDS             <1..>1241
                     /locus_tag="PLI55_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HPB01282.1"
                     /translation="LNNLSGEFMAVEWTETEKLQNPNRRGFVRPTPNGHALQYADGTP
                     FFMIGDTWLAGSTWRLPFRNADSPENYEPAPGIGFEDAVIYRKRQGFNSVSIISCFPN
                     WDADCKPSTYADKNGIFVRNAWEKFGYDVAEATGIDASGGLSYWGTLTAKEMRDEYGN
                     LPFAMSQIHKGVSDFNRINPQYFQSLDRKMEFLSQEGFVPLLETVRRDVGPSWYAYFD
                     FNETFARFVQYLIACYGAYNIIFSSIHLDWIPQEFSLTAEQFNEALTYHLEKYGPMPF
                     DQPVTTLISSSTYSVFGHHQDCPWLTMHSVGNKPRDHRVADSLEIIFRLEPPYPAINF
                     EPYYTGWDHEINMPNGERPPANSDRDNYFARAQMYGSVLSGGLSGHVHGTSAYDLTTT
                     GEPVGKRHHIWEALKFESANY"
BASE COUNT          310 a          281 c          306 g          344 t
ORIGIN      
        1 actaaacaac ctcagcggcg agtttatggc ggtcgaatgg acggaaaccg aaaaactgca
       61 gaatcccaac cggcgcggtt ttgttcgtcc tacacctaac ggtcacgcct tgcagtacgc
      121 tgatggaacg ccctttttta tgataggtga tacctggctg gctggctcga cctggcgact
      181 tcctttccgg aatgccgatt cgccggaaaa ttacgaacct gcgccgggaa tcggttttga
      241 agatgctgtc atatatcgca agcggcaggg cttcaattcc gtaagtatta tttcttgttt
      301 cccaaactgg gatgctgatt gtaagccgag tacctatgct gataaaaatg gaattttcgt
      361 cagaaatgcc tgggaaaagt tcggctatga tgttgctgaa gcaaccggaa ttgacgcttc
      421 cggtggtttg agctattggg gcacactgac tgccaaagag atgcgcgatg aatatggtaa
      481 tctgcctttt gcaatgtcgc aaattcataa aggcgtatca gacttcaatc gcatcaatcc
      541 gcaatatttt cagagccttg atagaaaaat ggaattttta tcgcaagaag gtttcgtccc
      601 cttattagaa actgtgcggc gggatgtcgg accatcttgg tatgcttatt ttgatttcaa
      661 tgagactttt gcgcgttttg tgcaatactt gatagcctgt tacggcgctt acaatattat
      721 tttcagcagt attcacctcg actggattcc gcaagaattc agtctgacgg cggagcaatt
      781 taacgaagct ttgacttatc atctggaaaa atatggaccg atgccttttg atcagccggt
      841 tacaactctt atcagcagtt cgacctattc ggtgtttggt catcatcaag attgtccgtg
      901 gttgacgatg cacagcgttg gtaataaacc gcgggaccac cgggtagccg attcgctgga
      961 aataattttt cgactcgagc cgccgtaccc ggcgattaat ttcgagccat attacactgg
     1021 ttgggatcac gagattaaca tgccgaatgg cgaacgtccg ccagccaatt ccgaccgtga
     1081 taattacttc gcgcgagcgc aaatgtacgg ctcggtactt tcgggtggtt tgtctggtca
     1141 cgttcacggg acttcggctt atgaccttac cacaactggc gaaccggtcg ggaaacgtca
     1201 tcatatttgg gaagccttga aatttgaatc ggcaaattat a
//
LOCUS       DAOOMU010000230         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_148758_length_1241_cov_8.430017, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000230 DAOOMU010000000
VERSION     DAOOMU010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148758_length_1241_cov_8.430017"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="PLI55_10405"
     CDS             <1..655
                     /locus_tag="PLI55_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB01283.1"
                     /translation="KPLTQKELASNLAGWKVMPGNKSNMIALVKPEIDAEKITDSERY
                     TIGVFDSRGNCRSVGKKEENCWYFTIVGNEDGESLNFKIWDAEKECALTADQKIQFVS
                     DALVGAPSEPLVVKFSGSEATDVSLTLPTKLTLEQNYPNPFNPVTAISYTIPEPGIVN
                     LKIYDLKGNLVKTLVDKFQDSGNYTVEWNAGDLSSGIYFYKIQYGGQSLIKRCLLIK"
     gene            773..>1241
                     /locus_tag="PLI55_10410"
     CDS             773..>1241
                     /locus_tag="PLI55_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01284.1"
                     /translation="MLSKNKLQFAQAFSYVQMRRRLAVFLLMLMLPLIIFANPPNWQP
                     ITGTQYSMIAMTEIRLYDHVFEGGGNNIAGAFGSGDESDCRGIGSWIPPNPPAHNGFW
                     YFTIVGNKNGEEITFKIYDEGTDAIYRCNPALTFVDNSTVGDPDLFDLSVQESA"
BASE COUNT          378 a          224 c          280 g          359 t
ORIGIN      
        1 taagccgttg actcaaaaag aacttgcatc taatttagcc ggttggaaag taatgccggg
       61 caataagagc aatatgatcg cactggtaaa gccggagatt gatgctgaaa aaataactga
      121 ttccgagaga tatacaattg gggtttttga tagccgcggt aattgtcggt ctgtcgggaa
      181 aaaagaggaa aactgctggt atttcactat tgtcggcaat gaggacggag aatcgcttaa
      241 ttttaagatt tgggatgcgg agaaagaatg tgccttaacc gctgaccaaa aaattcaatt
      301 cgtttccgat gctttagttg gcgcaccgag tgagccgtta gttgtcaaat ttagtggctc
      361 tgaagcgaca gacgtttctc taaccctgcc tacgaaatta acgctcgagc aaaattaccc
      421 gaatcccttt aatccggtta ccgcaattag ctacacgata cccgagccgg gtattgtcaa
      481 tttgaaaata tatgacttaa aaggtaatct ggtaaaaacc ctcgttgata aattccagga
      541 ttctggtaat tataccgtgg aatggaatgc cggagattta agttcaggaa tatacttcta
      601 caaaatccag tacggtggtc agtccctaat aaagagatgt ttattaatta aataggatct
      661 aagaatcttt acaataagga agtagggatt tgtgattatt ggattaattc tgtccttgaa
      721 tgtttaataa atcctaaaaa taccgtttac acggatttag gaggacaaga atatgttaag
      781 taaaaataaa ttgcagtttg cgcaagcttt tagctatgtt cagatgaggc gcagactcgc
      841 cgttttccta ttaatgctaa tgctcccact gatcattttt gccaatccgc cgaactggca
      901 accgattact ggcacgcaat attctatgat tgcaatgacg gaaattaggc tttatgacca
      961 tgtttttgaa ggcggtggaa ataacattgc cggggcattt ggttccggcg acgaatctga
     1021 ctgtagagga ataggttcct ggataccacc taatcctccc gcgcataatg gtttttggta
     1081 tttcacaatt gtcggtaaca aaaatggcga agagataact tttaaaatat atgatgaggg
     1141 gaccgatgcg atctatagat gtaatccggc cttgaccttt gttgataatt caacagttgg
     1201 tgatccggac ttattcgatc tctcggttca agaaagtgct a
//
LOCUS       DAOOMU010000231         1202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_156580_length_1202_cov_3.641674, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000231 DAOOMU010000000
VERSION     DAOOMU010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156580_length_1202_cov_3.641674"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PLI55_10415"
     CDS             complement(<1..943)
                     /locus_tag="PLI55_10415"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPB01285.1"
                     /translation="MATGTVKGRVFDKKTKEALPAANIIVSGTNIGTAADLDGNYFIN
                     YVPSGTQVLEVTYIGYEPIEVEVIVPEDGVVIQDLMLSPVAIKGEAVVITGQAASQGA
                     AINQQISSNTITNIVSSSKIRELPDESAAAALSRLPGVSLMEGDKVAIRGIQAKQNLV
                     LVNGVQLPSTDLEDRSTNLGFFSSNMLSGIEVVKVLTPDMDANAIGGVVNLRLREAPT
                     GFQSDVFAQGSYNTQDRTYPGQNYKFWASASNRFFNDKLGVFIQGNAERSNVGNDVTT
                     AGYTRDTGTEPYGEGIYQMKDFAFIDEENIITNYGGSV"
BASE COUNT          352 a          273 c          213 g          364 t
ORIGIN      
        1 aaacactccc gccatagtta gtaatgatat tttcttcatc aataaaagca aaatctttca
       61 tctggtaaat accctcgccg tatggttcgg tacctgtatc ccgggtatat ccggcagtgg
      121 ttacatcatt acccacatta gaacgttcgg cgttaccctg aatgaagact cccagcttat
      181 cattgaaaaa gcggttgcta gcactggccc aaaatttata attctgacca ggataggtac
      241 ggtcttgagt attatagcta ccttgggcaa atacatcaga ttgaaacccg gttggagctt
      301 ctctcaatct caaattaaca actccaccaa tcgcattggc atccatatcc ggagtaagca
      361 ctttaacaac ttcgataccg gaaagcatat tcgatgagaa gaagcccaaa ttagttgaac
      421 gatcttccag atcggttgac ggaagctgga caccattgac tagaaccaga ttttgtttcg
      481 cctgaatacc acggatagcg actttatcac cctccatcaa tgaaactcca ggtagacgac
      541 ttagagctgc tgctgcactt tcatccggca gttcgcgaat ttttgaacta gagacgatat
      601 ttgtaatagt attcgaagat atctgctggt tgattgcggc tccctggctg gcggcttggc
      661 ctgttattac aaccgcttcg cctttaatcg ccaccggtga aagcattaaa tcctgaataa
      721 ccacaccatc ttcaggtact attacttcaa cttcgattgg ttcataacca atataggtta
      781 cttcaagtac ttgcgtgcca gatggaacat aattaatgaa gtaattccca tctaaatcag
      841 cggctgttcc gatattggta ccacttacga taatattcgc cgctggcaac gcttctttgg
      901 tttttttatc aaacaccctt ccctttaccg taccagttgc cattaccaac tgaaccgtca
      961 ttagaaaaat tattcctacc ttgaaaagca taccattatg aatttggaaa cttgttctac
     1021 tactcttgag taaatagatt gtcagcttct taaaagtctc tgcatttttc ataaagctac
     1081 tatcctcttt ttattggttt aatattaatt gaatcgtttt aaaaagtcaa ggaaaatttt
     1141 atagaagaaa ttatggtaag ggtggatacg cacctatata accaataacc ttctctgaac
     1201 tt
//
LOCUS       DAOOMU010000232         1201 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_156731_length_1201_cov_11.466841, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000232 DAOOMU010000000
VERSION     DAOOMU010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156731_length_1201_cov_11.466841"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..>1201
                     /locus_tag="PLI55_10420"
     CDS             102..>1201
                     /locus_tag="PLI55_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HPB01286.1"
                     /translation="MAKKFFSASLILLVFSGLIFGAVINVPSNQPTIQAGIDAAVTGD
                     VVLVAEGTYKEKIDFKGKKITVASLFYNDGKTDHIQKTIIDGQVTGTVVKFSGGEDSS
                     SVLIGFTITNGSADYGGGIYCYNSSPTIKNVVIRDNVAKYSGAGIHCYSSSPTLERTV
                     IYNNKANESGGTGGGICCFNNSNPVLRNVTIAKNTAKSNGNDIACQYASKITIVNSII
                     WSEYLPDPIYIEGTGSISATYSDIKNGTGKSYFGEGCIATDPLFKDYNNNDFNLNYNS
                     PAIDAGNPDLDGDGTSWTDDVDDQDPDGTRLDMGAYYFVRTGIQGTITINGGGGKVED
                     AVIKATLTTAPSTSSTTKPDKDGKYFLQLSNG"
BASE COUNT          373 a          217 c          267 g          344 t
ORIGIN      
        1 aatcttataa aaaccttcaa ttggggcgta aatattttgt tagaaaaagc tatttatttt
       61 taaaaaaagg tttatattat aaaaaattat ggaggtgaag aatggcaaag aaatttttta
      121 gcgctagtct aattcttttg gtattttccg gactaatatt tggagccgtt ataaacgttc
      181 cgagtaatca gccgactatt caggctggga tagatgcggc agtaaccgga gatgttgtat
      241 tggtagctga gggcacttat aaggagaaga ttgacttcaa aggcaagaag atcacagtcg
      301 cctcactttt ttataatgat ggcaaaactg accacattca gaaaacaatc atagatggtc
      361 aagtgactgg aactgtggtt aaatttagtg gtggcgaaga ttcaagttca gtattgatcg
      421 gattcactat cactaatgga agtgctgact atggcggtgg tatttattgt tataattcca
      481 gccctacgat aaaaaatgtg gtcatccgcg ataacgtagc caaatatagt ggcgcgggaa
      541 tccattgcta ttcgtccagc ccaactttgg aaagaaccgt tatctataat aataaagcca
      601 acgaatccgg tggaactggc ggcggtatct gctgtttcaa taattctaac ccggtcttgc
      661 gtaatgttac tattgccaaa aacaccgcca aatcgaatgg taacgatatt gcctgtcaat
      721 atgcctcaaa gattacgatc gtaaactcga ttatctggag tgaatatcta cccgatccaa
      781 tttatattga aggaactggg agtatctccg cgacttattc ggatattaaa aatggtaccg
      841 gaaaaagtta ctttggtgaa ggatgtattg ctacagaccc gctttttaaa gattacaata
      901 acaatgattt taatcttaat tacaattccc cagctattga tgctggcaat cctgacctcg
      961 atggtgatgg cacctcctgg acggacgatg tagatgatca ggacccagat ggaaccaggt
     1021 tagatatggg ggcatattat ttcgttcgca ccgggattca gggaacgatt actattaatg
     1081 gtggcggcgg gaaggtagaa gatgcggtta taaaagcaac cctgactacg gctccttcga
     1141 cttcatctac caccaagcct gataaagacg gcaaatattt tctgcagtta tctaatggaa
     1201 g
//
LOCUS       DAOOMU010000233         1195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_157966_length_1195_cov_10.968421, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000233 DAOOMU010000000
VERSION     DAOOMU010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157966_length_1195_cov_10.968421"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLI55_10425"
     CDS             complement(<1..385)
                     /locus_tag="PLI55_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPB01287.1"
                     /translation="MLRPFERIQLVSGDITREKCDAIVNAANRSLLGGWGVDGAIHRA
                     AGPRLLEECRRIREQLLPDGLPTGEAILTAGYDLPARVVIHTVGPVWRGGEHGEDELL
                     AACYRNVLRLAAENKFDVIAIPAIST"
     gene            568..>1195
                     /locus_tag="PLI55_10430"
     CDS             568..>1195
                     /locus_tag="PLI55_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB01288.1"
                     /translation="MSSDNRLVFIKKLQMKYDPEPLHSNQVAKLSLQLFDELTPLHKL
                     SASERLLLEASALLHDIGYARQNADGHHKNSLRIIMEAGTPALPGFNPTETEIIANVA
                     RYHRKSLPSENHPYFAKLNSNARVIVCKLAALLRIADGLDRSHRSLVSKVLTFIEPEK
                     VIFDIFHFGELRNEINAAEKKADLFEIVYERQAVFRKQIIKIDDQTNRV"
BASE COUNT          346 a          297 c          241 g          311 t
ORIGIN      
        1 cggtactaat ggctgggatg gcgataacat caaatttatt ctcagccgca aggcgtaaaa
       61 catttcgata acaggcggct aagagttcat cctcaccgtg ttcgccaccg cgccaaaccg
      121 gaccgaccgt gtgaatcacg acacgagccg gcaaatcgta gcctgccgtc aaaatcgctt
      181 caccagttgg taaaccgtcc ggtaacaatt gctccctaat tcgacggcac tcttccaaaa
      241 gtcgcggacc agccgcgcga tgaatagcgc catcaactcc ccagccaccc aggagcgaac
      301 gattggcggc attgacgata gcgtcgcatt tttcccgagt aatatcaccg gagacaagtt
      361 ggattcgttc gaatggtctt aacattttca tcccggatct taatttattt tcatctgaaa
      421 aattcttttg cataatatac taatttgttg ttatttgtga gagcaatcat tatagttata
      481 tgttagcgaa ttctgtcgaa atagtcataa agccggggca tcctagcttt tatcccgaga
      541 ctaaaaaggc taaaccgaga tcaaatcatg agttctgata atcgactggt attcatcaaa
      601 aaactccaga tgaaatatga tccggaacca cttcattcca accaggttgc caaactgtcc
      661 ctgcaattgt tcgatgaact tactccttta cacaaacttt ctgcctccga acggctttta
      721 ctcgaagcct cagccctgtt gcatgacatc ggatatgcac gtcaaaatgc tgatggccat
      781 cataaaaata gcctgcgcat catcatggaa gccgggacac cggctttgcc gggattcaat
      841 ccaaccgaaa ctgaaatcat tgccaatgtc gcccgctacc accgtaaatc cctgccttca
      901 gaaaaccatc cttattttgc taagcttaat tcaaatgcgc gcgtaatagt ctgcaagttg
      961 gcggctttgc ttaggatagc agacgggctc gaccgcagtc accgcagttt agtttctaaa
     1021 gtcctcacct ttatcgaacc agagaaagta atcttcgaca tttttcattt tggtgaatta
     1081 cgcaatgaaa ttaatgcggc tgaaaagaaa gccgacttat tcgaaatagt ttatgaacgc
     1141 caggcggttt ttcgaaaaca aataatcaag attgatgacc agacaaatag agttc
//
LOCUS       DAOOMU010000234         1193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_158404_length_1193_cov_8.630053, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000234 DAOOMU010000000
VERSION     DAOOMU010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158404_length_1193_cov_8.630053"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLI55_10435"
     CDS             <1..520
                     /locus_tag="PLI55_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPB01289.1"
                     /translation="IGVFDSRGNCRSVGKKEEYCWYFTIVGNEDGESLNFKIWDAKKE
                     CELVTEQKIQFVSDALVGAPSEPLVVKFSGSEATDVSLTLPTKLTLEQNYPNPFNPVT
                     AISYTIPEPGIVNLKIYDLKGNLVKTLVDKFQDSGNYTVEWNAGDLSSGIYFYKIQYG
                     GQSLIKRCLLIK"
     gene            638..>1193
                     /locus_tag="PLI55_10440"
     CDS             638..>1193
                     /locus_tag="PLI55_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01290.1"
                     /translation="MLSKNKLQFAQAFSYVQMRRRLAVFLLMLMLPLIIFANPPNWQP
                     ITGTQYSMIAMVNIRLYDYVFEGGGNNIAGAFGSGGESDCRGIGSWIPPNPPAHNGFW
                     YFTIVGNKNDEEITFKIYDERTDAVYRCHPTLTFIDNSTVGDPDLFELSVQESAISGK
                     IKLLTTTPPAGDIVNVEISAGDVGV"
BASE COUNT          355 a          210 c          275 g          353 t
ORIGIN      
        1 gattggggtt tttgatagcc gcggtaattg tcggtctgtc gggaagaaag aggaatactg
       61 ctggtatttt acgattgtcg gcaatgagga cggagaatcg cttaatttta agatttggga
      121 tgcgaaaaaa gaatgtgaat tggttacaga acaaaaaatc caattcgttt ccgatgcttt
      181 agttggcgcg ccgagtgagc cgttagttgt caaatttagt ggctctgaag cgacagacgt
      241 ttctctaacc ctgcctacga aattaacgct cgagcaaaat tacccgaatc cctttaatcc
      301 ggttaccgca attagctaca cgatacccga gccgggtatt gtcaatttga aaatatatga
      361 cttaaaaggt aatctggtaa aaaccctcgt tgataaattc caggattctg gtaattatac
      421 cgtggaatgg aatgccggag atttaagttc aggaatatac ttctacaaaa tccagtacgg
      481 tggtcagtcc ctaataaaga gatgtttatt aattaaatag gatctaagaa tctttacaat
      541 aaggaagtag ggatttgtga ttattggatt aattctgtcc ttgaatgttt aataaatcct
      601 aaaaataccg tttacacgga tttaggagga caagaatatg ttaagtaaaa ataaattgca
      661 gtttgcgcaa gcttttagct atgttcagat gaggcgcaga ctcgccgttt tcctattaat
      721 gctaatgctc ccactgatca tttttgccaa tccgccgaac tggcaaccga ttactggcac
      781 gcaatattct atgattgcga tggtgaatat taggctttat gattatgttt ttgaaggcgg
      841 tggaaataac attgccgggg catttggttc cggcggcgaa tctgactgta gaggaatagg
      901 ttcctggata ccgcctaatc ctcccgcgca taacggtttt tggtatttta cgattgtcgg
      961 taacaaaaat gacgaagaga taacttttaa aatatatgat gaaagaaccg atgcggttta
     1021 tagatgtcat ccgaccttga catttattga taattcaaca gttggtgatc cggacttatt
     1081 cgagctctcg gttcaagaaa gtgctatctc agggaaaatc aagttactga caactacacc
     1141 accggcaggt gatatcgtaa atgttgaaat atcggcgggc gacgttgggg ttc
//
LOCUS       DAOOMU010000235         1191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_158809_length_1191_cov_17.590669, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000235 DAOOMU010000000
VERSION     DAOOMU010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158809_length_1191_cov_17.590669"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PLI55_10445"
     CDS             complement(<1..983)
                     /locus_tag="PLI55_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HPB01291.1"
                     /translation="MKAYFISEIKDLKTTETPLTIADVEQPRPTSNDILIKVSVCGIC
                     HTELDEIEGRTPPASFPMIPGHQVIGRIIENGPDARRFAIGTRVGVAWIFSACGKCSY
                     CLSNQENLCPQFRATGRDANGGYAEYMTVDENFAYPIPEIFTDAEAAPLLCAGAIGYR
                     SLRLTNLHNGQKLGLTGFGASAHLVLKMVRHRFPQTPVYVFARSKNEQAFARELGAVW
                     AGDTNEPASELLDAIIDTTPVWTPIVAALKNLKPGGHLVINAIRKENSDKSILTTIDY
                     PEHLWLEKEIKSVANVTRRDVAEFLELAAEIPIKPEVEIYPFEKANQALLD"
BASE COUNT          339 a          248 c          283 g          321 t
ORIGIN      
        1 aaatccagca gggcttgatt ggccttttca aacggataaa tttcgacttc cggtttgatc
       61 ggaatttccg cggctaattc taaaaattcg gctacatccc gccgagtgac attggcaacg
      121 gatttaattt ccttctccag ccagagatgt tcgggataat caatcgttgt cagaatggat
      181 ttatcgctat tttctttacg gatagcgttg ataaccagat gaccgcccgg tttgagattt
      241 ttcaaggcgg cgacgatggg agtccagacc ggcgtggtat cgataatagc atccagcaat
      301 tcggatgcag gttcattcgt gtcgcctgcc cagacggcgc cgagttcacg cgcgaatgcc
      361 tgttcatttt tactgcgggc gaagacgtac accggcgttt gcgggaaacg atgacgtacc
      421 attttaagaa ctaaatgagc cgaggcgccg aaaccggtta agcctaattt ctgaccgttg
      481 tgtaaattag tcagacgcaa agaacgataa ccaatcgcac cggcacagag aagtggggcg
      541 gcttcggcgt cggtgaaaat ttcaggaatt ggataagcga aattttcgtc aaccgtcata
      601 tattccgcgt aaccgccgtt ggcatcacgc ccggtagcgc gaaattgcgg acagagattt
      661 tcctgatttg agaggcagta ggagcatttt ccgcaggcgg aaaaaatcca ggcgacgccg
      721 acgcgagttc cgatagcaaa gcggcgggcg tcgggaccat tttctattat ccgaccgatt
      781 acctgatgtc ccggtatcat cggaaaggac gcgggcggtg tacgaccttc gatttcatcc
      841 agttcagtat gacaaatgcc gcagacactg actttaatca agatatcatt ggaagtcgga
      901 cgtggttgtt caacatcagc gatagtaaga ggcgtctcag tcgtttttaa atctttaatt
      961 tccgagataa aataagcttt caattggcac cttctatttc aatacgaatt taactatata
     1021 tcaaattatg tgaaacagag acattttgaa tccaaatagc tattgagtta aaaaataagt
     1081 ttaatagaaa aatgattgct atttttattc gagtaagcaa atacaatgta agtaactagc
     1141 ctgaataatt cataaaaata aagatgtttg gttgtttaat acattgtaat g
//
LOCUS       DAOOMU010000236         1177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_161891_length_1177_cov_12.602496, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000236 DAOOMU010000000
VERSION     DAOOMU010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161891_length_1177_cov_12.602496"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1177)
                     /locus_tag="PLI55_10450"
     CDS             complement(<1..>1177)
                     /locus_tag="PLI55_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPB01292.1"
                     /translation="YEYRMQVSVLDCTGCGNCADVCPGRKNEKALVMKPFESQLAQKE
                     NWEYFETIPVKANAMRKDTIKGSQFQQPLFEFSGACGGCGETPYLKVITQLFGDRMII
                     ANATGCSSIYSGSVVCPYCVNETGYGPAWANSLFEDNAEYGFGMALAIIQRREYLAEI
                     INEALKSADGELAEAFKAWLDGKDDAELSRAAGDRIKAALADKWEGNKLLTEIWKNRD
                     ILTKKSIWIIGGDGWAYDIGYGGLDHVIAMGYDVNILVLDTEVYSNTGGQSSKATPLG
                     SVAKFAYSGKKTSKKDLGMLAINYKNVYVASVAMGASANQFLKSALEAEKYPGPSLII
                     AYCPCINHGIYKGMLSQEESRQAVECGYWPLYRYNPLLKAEGKNPFQLESKEPNGKL"
BASE COUNT          320 a          297 c          243 g          317 t
ORIGIN      
        1 attagcttac cattgggttc cttgctttcg agctggaacg gatttttacc ttcggctttt
       61 agtaaaggat tataacggta aagcggccag taaccacact cgacggcttg tctggattct
      121 tcctgggaga gcattccctt gtaaatacca tgattgatgc agggacaata agcaattatc
      181 aaagaaggac ccggatattt ctcggcttcc agcgcggatt ttaagaattg gttggcgctg
      241 gcgcccatcg ccaccgaagc cacatagaca tttttataat taatggctaa cataccaagg
      301 tcttttttgg aagtcttttt accggagtaa gcaaatttgg cgactgaacc taaaggagtt
      361 gctttggacg actgcccgcc ggtattagaa taaacttctg tatcgagcac caaaatattg
      421 acgtcatatc ccatagcaat aacgtgatcg agaccaccgt agccgatatc ataagcccaa
      481 ccatcaccgc caataatcca gattgatttc ttagtcaaaa tatcacgatt tttccagatc
      541 tcggtcaata gcttattacc ttcccattta tcagccagag cggctttgat ccgatcgccc
      601 gccgcgcgcg agagttcggc atcatcttta ccatcaagcc aggctttaaa agcttccgcc
      661 agttctccat cggctgattt gagtgcctca ttaataattt ccgccagata ttcacgacgc
      721 tgaataatgg ctaaagccat tccgaaacca tattcggcat tgtcttcaaa gagggaatta
      781 gcccaggctg gtccatatcc tgtttcattg acacaataag gacaaaccac gctaccgcta
      841 taaattgacg aacaaccagt ggcattcgcg ataatcatac ggtcgccgaa taattgagtg
      901 atgactttga gataaggtgt ttcaccacaa cctccacagg cgccggaaaa ttcaaaaagc
      961 ggttgctgga actgactacc cttgatggta tctttccgca ttgcattagc cttgaccgga
     1021 atggtctcaa aatattccca gttctctttc tgtgccagct gagactcaaa cggcttcatc
     1081 actagagctt tttcattttt acgtcccggg cagacatcgg cgcagttacc gcaaccggtg
     1141 caatccagca ccgagacctg catacgatac tcgtaat
//
LOCUS       DAOOMU010000237         1159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_165869_length_1159_cov_7.811594, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000237 DAOOMU010000000
VERSION     DAOOMU010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_165869_length_1159_cov_7.811594"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PLI55_10455"
     CDS             <1..1088
                     /locus_tag="PLI55_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPB01293.1"
                     /translation="EISAEVAIAELLKTGCTTTSDHLYLFPAQADANLLDMTINVARE
                     MGIRFQPTRGSMSLGQSRGGLPPDNIIQKESEIVADIRRLIKLYHDDSSGAMTRIALA
                     PCSPFSVTIELMQMVVKLADEFNLQIHTHLAETLDEEKFCIENYGLRPFALLKNLGWI
                     RPNAWFAHSIYLNESEIAEMGAVGCGVSHCPTSNMRLGSGIAQIREMLDAGVNVSLGV
                     DGSASNDSSNMLLELRNAMLVSRLRQKDEWLTTDEILWMATRGGAAVLGRDDIGQLAE
                     GKCADLFLVSIDRIELAGAQHDPLAALIFAVLLSPVDYVIVNGRLIIEESQLMTADEQ
                     NLIKCHQRISDELIMRAERKTGKKFRR"
BASE COUNT          320 a          218 c          288 g          333 t
ORIGIN      
        1 tagaaatcag tgcagaggtc gcaatcgccg agttactcaa aaccggttgt acaacaacat
       61 cggatcatct ttatcttttt ccagcccagg ctgatgctaa tctgctggat atgacgatta
      121 atgtcgcacg tgaaatgggg atccgttttc agccaacgcg cggttcaatg tcgcttggtc
      181 aatcgagagg tggtttacca cctgacaata ttattcagaa agagagtgag atcgtagctg
      241 acattcgtcg gcttataaaa ttatatcatg atgactcatc gggggcgatg acgcggattg
      301 cccttgcgcc atgttcacct ttttcggtta cgattgagct aatgcaaatg gtagtaaaat
      361 tagccgatga atttaattta caaatacata ctcatttggc cgagacgctc gatgaggaaa
      421 agttttgtat agaaaattac ggcttgaggc ctttcgcttt actgaaaaat ctgggatgga
      481 ttcgacccaa tgcctggttt gcgcattcga tttatttgaa tgagtctgaa atcgccgaaa
      541 tgggggcggt tggatgtgga gtaagccatt gtccaacgtc aaatatgcga ctcggatcgg
      601 gaattgctca aattcgcgag atgctagatg ccggagtcaa tgttagcctc ggcgtagatg
      661 gttcggcatc gaatgactct tcgaatatgc ttttagaatt gcggaatgct atgctggttt
      721 ctcgattacg gcaaaaggac gaatggttga cgactgacga aatattatgg atggcgactc
      781 gcggtggtgc ggcggtactg ggacgcgatg acatcggtca attagctgaa gggaagtgcg
      841 ctgatctctt tctggtttca attgatcgga tagaattagc cggtgctcag catgatccgc
      901 tggcggcatt gatattcgca gttctactct cgcctgttga ttacgtcata gtcaatggtc
      961 ggcttataat tgaagaaagc caattaatga ccgctgatga acagaacttg attaaatgtc
     1021 atcaacgtat ttctgatgag ctgattatgc gagccgaacg taaaactggg aaaaaattcc
     1081 gtcgctaaaa tataaaaata atccttttca aaattatata ggagattaat atgaagcaat
     1141 ctgttgtttg taaaatttt
//
LOCUS       DAOOMU010000238         1154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_167035_length_1154_cov_22.547771, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000238 DAOOMU010000000
VERSION     DAOOMU010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167035_length_1154_cov_22.547771"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLI55_10460"
     CDS             <1..735
                     /locus_tag="PLI55_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003370833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPB01294.1"
                     /translation="HPFTGIDYIAYYRSLVEGEKAECTIIFSNQPKTILSYTKKVLAC
                     DVHTRERTKRQLIAAGAEIVYSLAEILSAPVNGSGYNPEYGLLGSNKATEETVKLFPR
                     NCQPVVDRVQQIFKSKTGKMVEVLIYGDGAFKDPVGKIWELADPVVSPAYTAGLKGTP
                     NELKLKYLADNQFADLTGPALEEAIAKQIRNKKENLVGSMESQGTTPRRLTDLIGSLC
                     DLTSGSGDKGTPVVHIQGYFDSFASE"
BASE COUNT          334 a          208 c          295 g          317 t
ORIGIN      
        1 catcctttta ctggaataga ttatattgct tattatcggt cattggtaga aggagaaaag
       61 gctgaatgca cgataatttt ttccaaccag cccaaaacga ttctgtcata cacgaaaaag
      121 gttctggctt gtgatgttca tacccgagag cgaaccaagc gacagttaat tgccgccggt
      181 gcggaaattg tctatagcct ggctgaaatt ttatcagctc ccgtgaacgg tagtggctat
      241 aatccggaat acggactgct gggttcgaac aaagctaccg aagaaaccgt gaagttattt
      301 ccccgcaatt gccaaccggt ggtagatcgc gttcagcaaa ttttcaaatc aaaaaccggg
      361 aagatggtcg aagtattgat ctacggcgat ggtgctttca aagacccggt tggaaaaata
      421 tgggaattag cagatcccgt agtctctccg gcttatactg caggtttgaa aggtacgccc
      481 aatgaattaa agctgaaata tctcgccgat aatcaattcg ccgacctgac tggtccggct
      541 ttagaagaag cgattgctaa acaaattcgg aataaaaaag agaatctggt tggctctatg
      601 gaatcccagg ggacaacgcc gcgccggctg accgacttga ttggatcgtt gtgtgaccta
      661 acctctggaa gtggcgataa aggtacaccg gttgttcata ttcagggata tttcgatagc
      721 ttcgccagcg aataaataac cggggcattg ggcattgggc attggtcact ggtcattggt
      781 cattagtcat tggtcattag tcattagtaa agaggctgag taaagagaaa agagactgag
      841 aaatggagaa aaggagacag gagacagttt tgcggaaatt ggtttagcta tttgtgattt
      901 ggaatttggg gtttgggatt ttactcattt agcggttctt ggcgaacttg gcggtttatt
      961 cttccaagac ccaaaacaaa aaaataaggc agagaaaggt cattggtcat tggtcattgg
     1021 tcattggtca ttggtaattg gtcactagtc attggtcatt ggtaattggg cattggtcat
     1081 tggtcattgg taaagaggct gagtaaaaag attgaggttg agaaaaaaat tcaaatctta
     1141 gccttttttc tctg
//
LOCUS       DAOOMU010000239         1151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_167713_length_1151_cov_23.375912, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000239 DAOOMU010000000
VERSION     DAOOMU010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_167713_length_1151_cov_23.375912"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1151)
                     /locus_tag="PLI55_10465"
     CDS             complement(<1..>1151)
                     /locus_tag="PLI55_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPB01295.1"
                     /translation="IYVYDDNVIRFRYTNQKEFSSAPSYAVIAALPAQSAFTFKDEGK
                     NFTLTTKALTVRITKSPCRVAIFDSAGNLINADEEAFGVSFDNDEVRCHKKLLPGENF
                     YGLGEKSDDLCKTGKEYILWNTDFWAYDDRTDPLYISIPFFYGIRDFKAYGILFDNTC
                     KSHFNFGASNNRFYWFGAEKGEMDYYFIYGPEIKKVLTAYTNLTGRAELPPLWALGYQ
                     QCRWSYYPESEVRNLAATFRRKKIPCDVIYLDIDYMDGYRVFTWNKKYFPEPAKMLAD
                     LKKDGFKVIPIIDPGVKADTNYFGAREGLKQDLFVKYPDGQYYQGEVWPSWAYFPDFT
                     KAATRQWWGDKLNLLLKDGVAGFWNDMNEPTVWGTHFPDIVQFDGNGLKT"
BASE COUNT          299 a          268 c          288 g          296 t
ORIGIN      
        1 gtttttaaac cgttgccatc gaactgcaca atgtcgggaa agtgcgtgcc ccaaacggtc
       61 ggctcattca tatcgttcca gaaaccggcg acgccatctt tgagcagtaa attgagttta
      121 tcgccccacc attggcgcgt ggcggcttta gtaaaatccg ggaaatatgc ccaggagggc
      181 cagacctcgc cctgatagta ttgaccgtca ggatatttga cgaataaatc ctgtttgagg
      241 ccttcgcgcg ccccaaaata attcgtgtca gctttgacgc ccgggtcaat tatcgggatg
      301 actttgaagc cgtccttttt taaatcagcc agcattttag ccggttcggg aaaatatttt
      361 ttgttccagg taaagacccg atagccgtcc atataatcaa tgtcgagata aatcacatcg
      421 caggggattt ttttacggcg aaaagtcgcc gccagattgc gtacctccga ttcaggataa
      481 tagctccagc ggcattgctg atagccgagt gcccagagcg gcggcagttc agcccgtccg
      541 gtcagattgg tataagccgt caggactttt ttaatttcag gaccgtagat gaaataataa
      601 tccatctcgc ccttttcggc accgaaccag tagaagcgat tattgctggc gccgaaattg
      661 aagtgcgatt tacaggtatt gtcaaataaa atgccatagg ctttgaaatc gcgaatccca
      721 taaaagaagg gaattgaaat gtaaagcggg tcggtgcggt cgtcataagc ccaaaaatcg
      781 gtgttccaga ggatatattc cttgccggtt ttgcaaagat cgtcgctctt ttcgcccagc
      841 ccgtaaaaat tttcaccggg caggagcttt ttgtggcaac gcacttcgtc attgtcgaag
      901 ctgacgccga aagcttcttc atcggcattg atcaaattgc cagccgaatc gaaaatggcg
      961 acgcggcaag gcgacttggt gattcgcacg gtcagggctt tggtggtcag tgtgaaattt
     1021 ttaccttcgt ctttaaacgt gaaagccgat tgggcgggca gagcggcgat taccgcatac
     1081 gacggcgcag atgaaaattc tttctgattc gtataacgaa aacgaatgac attgtcgtcg
     1141 taaacataga t
//
LOCUS       DAOOMU010000240         1140 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_170351_length_1140_cov_5.807373, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000240 DAOOMU010000000
VERSION     DAOOMU010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_170351_length_1140_cov_5.807373"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1140)
                     /locus_tag="PLI55_10470"
     CDS             complement(<1..>1140)
                     /locus_tag="PLI55_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016778074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPB01296.1"
                     /translation="IPGAENQLAIRLDNPNHSSRWYPGGGIYRNVWLVKTNPIHIAQW
                     GTYITAENISATCATLHLEITIDNDSRNDANVKAITEVFVLNENGDKIGKPVANFKPM
                     EIIIFTGTRTSEKNSIMIKNPKLWGPPPTQTPNCYVAVTTLWQDDKPIDQYETRFGIR
                     SVRFEPDSGIYINSQLIKIKGVNQHHELGALGAAFNTRAAERQLEILREMGCNAIRTA
                     HNPPAPELLELTDRMGFLVMDEAFDVWERKKTPLDFHLIFPEWYEPDLRAMVRRDRNC
                     PSVMIWSIGNEVGEQYTDEAGAALAKHLCEIVKEEDPKRPATSAMNWAKPYMPFPAVL
                     DLISLNYQGEGIRQEPEFEGTDRIRTTPQYDAFHEKFPDKVILSS"
BASE COUNT          279 a          277 c          264 g          320 t
ORIGIN      
        1 cgctactgag aatgacttta tccggaaatt tctcgtggaa agcatcatat tggggtgttg
       61 tgcgaatgcg gtcagtgcct tcgaattccg gctcctgacg aattccttcg ccctggtaat
      121 tcaggctaat gagatcaagt accgccggaa acggcatata cggtttcgcc cagttcatcg
      181 cgcttgtcgc cggacgctta ggatcttctt ccttgacaat ctcacataaa tgttttgcca
      241 gtgcagcgcc cgcctcatca gtatattgtt caccgacttc attgccgata ctccagatca
      301 tcactgacgg acaattgcga tcacgccgca ccatggcgcg caaatccggc tcataccatt
      361 ctggaaaaat aagatgaaag tctaacggag tctttttccg ctcccaaacg tcaaacgctt
      421 catccattac tagaaaaccc atcctatctg tcaattccaa aagttccggg gcaggtggat
      481 tgtgcgcggt gcgaatcgca ttacaaccca tttcccttaa aatctcgagc tggcgttcgg
      541 cggctcgggt attgaaagcc gccccgagag cgccgagttc atggtgctga ttgaccccct
      601 taattttgat gagttggcta ttaatataaa ttccggaatc cggttcgaaa cgcacggaac
      661 gaatgccaaa acgcgtttcg tactgatcaa tcggtttgtc atcctgccat agagtagtaa
      721 ccgcaacgta gcaattaggt gtctgagtag gcggcggtcc ccatagtttt ggatttttga
      781 tcataattga atttttttca ctggttcgag taccggtgaa aattataatt tccattggtt
      841 tgaaattagc gactggctta ccgattttat caccattttc atttaagaca aaaacttcgg
      901 taatcgcttt tacatttgcg tcgttccggg aatcattgtc tattgtaatt tccaggtgga
      961 gcgtcgcgca ggtcgccgaa atattttcag ccgtaatata agtaccccat tgggcgatat
     1021 ggatcggatt ggttttgacc agccagacat tgcggtagat tccaccgccg gggtaccagc
     1081 gcgacgaatg attgggattg tcaagacgaa tcgctagttg attctcagca cccggtatga
//
LOCUS       DAOOMU010000241         1133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_172041_length_1133_cov_10.902597, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000241 DAOOMU010000000
VERSION     DAOOMU010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_172041_length_1133_cov_10.902597"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLI55_10475"
     CDS             complement(<1..371)
                     /locus_tag="PLI55_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HPB01297.1"
                     /translation="MQKNFSDENQLRSVTKILETFERIQLVSGDITREKCDAIVNAAN
                     RSLLGGGGVDGAIHRAAGPRLLEECRQIREQLLPDGLPTGEAILTAGYDLPARFVIHT
                     VGPVWHGGEHGEDELLAACYR"
     gene            506..>1133
                     /locus_tag="PLI55_10480"
     CDS             506..>1133
                     /locus_tag="PLI55_10480"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPB01298.1"
                     /translation="MSSDNRLSFVKNLQMKYDPEPLHSNQVAKLSLQLFDELTPLHKL
                     STSERLLLEASALLHDIGYASQNADGHHKNSLRIIMEAGTPALPGFNPTETEIIANVA
                     RYHRKSLPSESHPHYAKLDSNSRSIVCKLAALLRIADGLDRSHRSLVSKVLTVIEPEK
                     VIFDIFHFGELRNEITAAEKKADLFEIVYKRQAVFRKQIIKIDDQTNRV"
BASE COUNT          323 a          285 c          225 g          300 t
ORIGIN      
        1 tttcgataac aggcggctaa gagttcatcc tcaccgtgtt cgccaccgtg ccaaaccggg
       61 ccgacagtgt gaatcacaaa acgagccggc aagtcgtagc ctgccgtcaa aatcgcttca
      121 ccagttggta agccgtccgg taataattgc tccctaattt gccgacactc ttccaaaagg
      181 cgcggaccag ccgcacgatg aatagcaccg tccactcccc cgccacccaa aagcgaacga
      241 ttggcggcat tgacgatagc gtcgcatttt tcccgagtaa tatcaccgga gacaagttgg
      301 attcgttcga atgtctctaa tattttcgtc actgatctta attgattttc atctgaaaaa
      361 ttcttttgca taatatacta atttgttgtt atttgtaaga gcaatcatta tggttatatg
      421 ttagcgaatt ctgtcgaaat agtcataaag ccggagcatc ctagctttta tcccgagact
      481 aaagaggctt aaccgagatc aaattatgag ttctgataat cggctgtcct tcgttaaaaa
      541 tctccagatg aaatatgatc cggagccatt gcattccaac caggttgcca aactgtctct
      601 gcaattgttc gatgaactta ctcctttaca caaactttct acctccgaac ggcttttact
      661 cgaagcctct gccctgttgc atgacatcgg atatgcaagc caaaatgccg atggccatca
      721 taaaaatagc ctgcgcatca tcatggaagc cgggacaccg gctttgccgg gattcaatcc
      781 aaccgaaact gaaatcattg ccaatgtcgc ccgctaccac cgtaaatccc tgccttcaga
      841 aagtcacccc cattacgcta aacttgattc aaattcgcgc tcgatagtct gtaaactggc
      901 ggctttgctt aggatagcag atggactcga ccgcagtcac cgcagtttag tttctaaagt
      961 cctcaccgtt atcgaaccag agaaagtaat cttcgacatt ttccattttg gcgaattacg
     1021 caatgaaatt actgcggctg aaaagaaagc cgacttattc gaaatagttt ataaacgtca
     1081 ggcggttttt cgaaaacaaa taatcaagat tgatgaccag acaaatagag ttc
//
LOCUS       DAOOMU010000242         1121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_175001_length_1121_cov_5.960600, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000242 DAOOMU010000000
VERSION     DAOOMU010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_175001_length_1121_cov_5.960600"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1015
                     /locus_tag="PLI55_10485"
     CDS             71..1015
                     /locus_tag="PLI55_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPB01299.1"
                     /translation="MIVPVKPPKFVIFLFIAILFSKSLYAMNPLLHYPCVPSDFGILY
                     REVSFKTDDGLVLKGWLYPPQDTTGLSKDFIGRMMAMPDSRKPERKEYHYSNSHNPTI
                     IICDGDAGNMAFSIFYAYHYFTNGYAVFTFDWRGFGESAPWKIDQDQLCCAEFLTDYN
                     AAINFIKDQPEVDSAKIGLMGFSTGAYLSFAMIASRDDISAYVGRALITSLNDLTTNL
                     QKVSPNRNFVIPQNYPENLLPLKAANKVRTPVFLIVGEKDDRTPVWMSKKVYKRLKGP
                     KELWIVPEAEHGGAKGPEMITYPEFFTKTLSFYNKYLK"
BASE COUNT          330 a          229 c          221 g          341 t
ORIGIN      
        1 taccgaatcg caatcctctt tcatcttggc ggattagata tgattccaca ctaaaatcgg
       61 aagtgcccaa atgatagtac ctgtgaaacc accaaaattc gtaattttct tattcatcgc
      121 tattcttttt tcaaaatctc tttatgcaat gaaccctctt ctgcattatc cgtgtgtgcc
      181 ttccgatttt ggaatcctct accgcgaggt ttctttcaag accgatgacg gattggtttt
      241 gaaaggatgg ctctatccgc ctcaggatac cactggactt tctaaagatt ttatcggaag
      301 aatgatggcg atgcccgaca gtcgtaaacc ggagagaaaa gaatatcatt attctaatag
      361 ccataatcct accattatta tatgcgatgg tgatgccggt aatatggcct tttcgatatt
      421 ctatgcttat cactatttta caaacggtta cgctgtattc acttttgact ggcgggggtt
      481 cggtgaaagt gctccgtgga aaattgatca agaccagctt tgctgcgcgg agttccttac
      541 cgattacaat gcggctatta attttattaa agaccaaccg gaagttgact cggcaaaaat
      601 tggcttaatg ggattttcca ccggcgccta cctctcattt gcaatgattg cctcaagaga
      661 tgatatttct gcctacgtcg gaagagcgct tattacttcg ctcaatgacc tcacgacgaa
      721 tttgcaaaaa gtcagcccga ataggaattt tgtgattcca caaaattatc ctgagaacct
      781 acttccgcta aaagctgcga ataaggtaag aacgcccgtc tttttaatag tcggcgaaaa
      841 agatgataga acgccggtct ggatgtcaaa gaaagtatat aagagactga agggtcctaa
      901 agagttatgg attgttcccg aagcagaaca tggaggcgca aaagggcctg aaatgataac
      961 ctatcctgag ttttttacta aaacactttc tttttataat aaatacttaa agtaatccga
     1021 atgttttttg attacacgat agtagataaa aagtctgggt ttcattttac ccaaattccg
     1081 ttatcagcgg aacttttaaa aattcgaata cattgtattc a
//
LOCUS       DAOOMU010000243         1113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_176969_length_1113_cov_16.350662, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000243 DAOOMU010000000
VERSION     DAOOMU010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_176969_length_1113_cov_16.350662"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLI55_10490"
     CDS             <1..194
                     /locus_tag="PLI55_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPB01300.1"
                     /translation="CYYCGKKFKPQELTMDHIVPIARGGKSIKRNVVPACKECNNKKK
                     YLTPVEILMNRKDKSSPEK"
     gene            complement(322..>1113)
                     /locus_tag="PLI55_10495"
     CDS             complement(322..>1113)
                     /locus_tag="PLI55_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPB01301.1"
                     /translation="KAIRVLIDTVLVFDSNTSLTEAKNKTLAFARECAIKKAVPEEVM
                     TSSVLTDLRAESGRQAQEQTAINIFAISTQAGYIIEEKVISSEPIFTNDILHYRLKLK
                     ALVEPTRGERDPSLSLELSVNRNVLKDGDPLFITAKSSTDGYLYLFNFLADNSVLLMF
                     PNPISPDNAIKAGIPLQIPTIAEQKKGVYYKVQADPNKPVTAEAIFGVFCKNRIAEID
                     KFQTVHKDFVVLSAGEQSFTDFQKWLVRVPLSQRIEKAIPIQIFK"
BASE COUNT          321 a          218 c          267 g          307 t
ORIGIN      
        1 tatgctatta ctgcggcaag aaatttaagc cgcaggaatt aacgatggat catattgtac
       61 cgattgcccg cggcggaaaa agcatcaaaa gaaacgtcgt ccctgcctgc aaagaatgca
      121 ataataagaa gaaatatcta acgccggtgg aaatattgat gaaccggaaa gataaatcat
      181 ctcccgaaaa atagttcatt aaggttgagt taagttctga gaagatcaat ttgaaaatct
      241 caatttttgt caagccgagg tatcagtttt tcaattgaaa gaaagtggag cgaattcagc
      301 ttaactcaag tttcgcttta ttcatttgaa aatctgtatc gggatggctt tttcaatgcg
      361 ctgggaaagc ggcacgcgca ccagccattt ctggaaatcg gtgaaacttt gttctccagc
      421 ggatagtact acgaagtcct tatgcacggt ttgaaattta tcaatctcgg cgattcgatt
      481 tttacaaaat accccgaaga tggcttcggc cgtaaccggt ttattgggat cagcttgaac
      541 tttataataa acgccctttt tttgttcagc gatagttgga atctggagcg gaatcccggc
      601 tttaatggca ttatctggtg atattggatt cgggaacatt agtagaaccg aattgtccgc
      661 taaaaaattg aaaaggtaga gatagccgtc ggtagaagat ttagccgtga tgaacagcgg
      721 atcgccatct ttcaggacat tgcgattgac ggataattcg agcgacaatg acggatcgcg
      781 ttcgccgcga gtcggctcga ccagagcctt aagctttagg cgataatgca aaatgtcatt
      841 ggtgaatatc ggctcggatg agattacttt ctcttcaata atatagccag cttgggtcga
      901 aattgcgaaa atgttgatcg ccgtctgttc ctgcgcttgg cggccagatt cggctcgcaa
      961 atccgttagg acggaagagg tcattacttc ctcaggaacg gctttcttga tggcgcactc
     1021 acgggcaaag gcaagagtct tatttttggc ttcggttagg gatgtattac tatcaaaaac
     1081 cagaaccgta tcaattaaaa cgcggatggc ttt
//
LOCUS       DAOOMU010000244         1098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_180893_length_1098_cov_10.710451, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000244 DAOOMU010000000
VERSION     DAOOMU010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_180893_length_1098_cov_10.710451"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLI55_10500"
     CDS             <1..364
                     /locus_tag="PLI55_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPB01302.1"
                     /translation="SPRIHSWDRIAMAGQMTNKSQIPKFKSQTNPNYQNSNDPNLRYG
                     LKNISGWEAKSQRKKWNFQRCRKTLTTKTQRHEVLVKRLRMRMVISHWSLVISKEAKA
                     EKRRKEAGRQEAGDSFDD"
     gene            complement(600..974)
                     /locus_tag="PLI55_10505"
     CDS             complement(600..974)
                     /locus_tag="PLI55_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019941375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPB01303.1"
                     /translation="MKLEELRVYQLAMEIGDLVWAIVQKWDYFPKDTLGKQLVRSADS
                     IAANLSEGFGRYYFREARQFGYYARGSLSETKTWLEKAHNRSLMDDKDFEKIKGDFDL
                     IGKQLNSYIKSIGNQQTNTTSQ"
BASE COUNT          333 a          223 c          225 g          317 t
ORIGIN      
        1 tagcccacga attcattcgt gggatagaat tgcgatggcg ggacaaatga caaacaaatc
       61 ccaaatccca aaattcaaat cccaaacaaa tcctaattac caaaattcca atgatccaaa
      121 cctacgatac ggccttaaga acatctccgg ctgggaagcg aagtcccagc ggaaaaaatg
      181 gaattttcag cgctgtcgga aaactttaac cactaagact caaagacacg aagttttagt
      241 aaaaaggctg agaatgagaa tggtcattag tcattggtca ttggtcatta gtaaagaggc
      301 taaggctgag aaaaggagaa aggaggcagg gagacaggag gcaggagaca gtttcgacga
      361 ctgaaattaa tcgttccaat ttaccgttgc ggcatcaaga atattgcact ggatttcggg
      421 tttgtgattt ggaatttgtg atttgggatt tggagtttgg gatttttctc atttagcggt
      481 tcttggcgaa cttggcggtt tattcttcta agactaagag gaaagagact gagaaaagag
      541 gtcgagaatg agaatggtca ctggtcattg gtcattggtc attggtcatt agtcactggt
      601 cattgggatg tggtgttagt ttgttgattg ccgattgatt ttatatatga gtttaattgt
      661 ttaccaatca aatcaaaatc acctttgatt ttttcaaaat ctttatcatc cattaagctt
      721 ctattatgag ctttttctaa ccaggtttta gtttcggata aagagcctct cgcataatat
      781 ccgaattgtc ttgcctctct aaaataatac cttccgaatc cctcactaag attggcagct
      841 atcgaatccg cggatctcac caactgcttg cctaatgtat ccttgggaaa ataatcccat
      901 ttctgaacga tggcccagac taaatcccct atttccattg ccagctgata aacgcgtaat
      961 tcttccaact tcacctctct tttcctccta taaattaata tttttaataa tcaccaataa
     1021 ccaatgacta atgaccaatg cccaatgacc accttagaac cccgacgtcg gtggcgagac
     1081 aaatgccact tcggcgga
//
LOCUS       DAOOMU010000245         1076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_186804_length_1076_cov_14.908913, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000245 DAOOMU010000000
VERSION     DAOOMU010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_186804_length_1076_cov_14.908913"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PLI55_10510"
     CDS             <1..792
                     /locus_tag="PLI55_10510"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HPB01304.1"
                     /translation="KAIRVLIDTVLVFDSNTSPAEAKNKTLAFARECAIKKAVPEEVM
                     TSSVLTDLRAESGRQAQEQTAINIFAISTQAGYIVEEKVISSEPKFANDILHYRLKLK
                     ALVEPTRGERDPSLSLELSVNRNVLKDGDPLFITAKSSTDGYLYLFNFLADNSVLLMF
                     PNPISPDNAIKAGVPLRIPTAAEQKRGISYKVQADSNKLVTAEAIFGVFCKNRIAEID
                     KFQTVRKDFVVLSAGEQSFTDFQKWLVRVPLSQRVEKVIPIQIFK"
     gene            complement(883..>1076)
                     /locus_tag="PLI55_10515"
     CDS             complement(883..>1076)
                     /locus_tag="PLI55_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPB01305.1"
                     /translation="CYYCGKKFKPQELTMDHIVPIARGGKSIKSNVVPACKECNNKKK
                     YLTPVEILMNRKDKSTPEN"
BASE COUNT          280 a          262 c          232 g          302 t
ORIGIN      
        1 aaagccatcc gcgttttaat tgatacggtt ctggtttttg atagtaatac atccccggct
       61 gaagccaaaa acaaaaccct ggcctttgcc cgcgagtgcg ccatcaaaaa agccgttcct
      121 gaggaagtaa tgacctcctc cgtcctgacc gatttgcgag ccgaatccgg tcgccaggcg
      181 caggagcaga cagcaatcaa cattttcgca atttcaactc aagcgggcta cattgtagaa
      241 gagaaagtca tctcatctga gccgaaattc gccaatgata ttttgcatta tcgcctaaag
      301 cttaaggctc tggtcgagcc gactcgcggc gaacgcgatc cgtcattgtc gctcgaatta
      361 tccgtcaatc gcaatgtcct gaaagatggc gatccgctgt tcatcacggc taaatcttct
      421 accgacggct atctctacct tttcaatttt ttagcggaca attcggttct actaatgttc
      481 ccgaatccaa tatcaccaga taatgccatt aaagccggtg tccctctgcg gattccgact
      541 gccgccgaac agaaaagagg catttcctat aaagtccagg ctgattccaa taaattggta
      601 acggctgaag ccattttcgg agtgttttgt aaaaaccgaa tcgctgagat tgataagttt
      661 caaaccgtgc gcaaggattt cgtagtgctg tctgccggag agcagagttt taccgatttc
      721 cagaaatggc tggtgcgcgt gccgctctcc caacgcgtcg agaaagtcat ccccatccag
      781 attttcaaat aaaagtaaaa cttgggttaa gccaagctag aaaagctttg tctcaatcga
      841 aaatccaatg ccttggctta acataagttg aatatcgctt aattaatttt cgggagttga
      901 tttatctttc cggttcatca atatttccac tggcgttagg tatttcttct tattattgca
      961 ttctttgcag gcagggacga cgttgctttt gatgcttttt ccgccgcggg caatcggtac
     1021 gatatgatcc atcgttaatt cctgcggctt aaatttcttg ccgcagtaat agcata
//
LOCUS       DAOOMU010000246         1041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_197122_length_1041_cov_15.677485, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000246 DAOOMU010000000
VERSION     DAOOMU010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_197122_length_1041_cov_15.677485"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PLI55_10520"
     CDS             <1..1008
                     /locus_tag="PLI55_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPB01306.1"
                     /translation="GCGRISKNHFESINQLSNDLKLVAVCDIIEERARAAGEKYGVDW
                     YTDYDKMLERDDIDIISICTPSGLHPEHGIKAAQKGIHVISEKPMAISLAGADALIKA
                     CDDNKVQLFVVKQNRLNTTSQLLKRAVDKGRFGKIYMAQTNVFWQRPQEYYSQAKWRG
                     TWEFDGGSFMNQASHYVDALLWLLGPADHVMAETATMARKIETEDTGSAIIKFRNGAI
                     GTINVTMLTYPKNFEGSITILGEKGTAKLGGVAINKIEKWEFEDYDDDDRLVHEANYN
                     PPNIYGLGHLPYYKNVIAVLNGDEEANTDGRSGRKSLELIIAIYKSAQTGQKVALPLK
                     L"
BASE COUNT          320 a          216 c          246 g          259 t
ORIGIN      
        1 ggttgcggtc gaatttctaa aaatcacttt gaatccatta atcaattgtc taatgacctg
       61 aaattagtag cggtctgcga tattattgag gaacgcgccc gcgccgccgg tgaaaaatac
      121 ggagttgatt ggtacactga ttatgataaa atgctcgaaa gagatgatat tgatattatt
      181 tcaatctgta cgcccagcgg tctgcatccg gaacacggca ttaaagccgc tcagaaaggc
      241 attcatgtca tttccgaaaa accgatggct atttcactgg cgggcgctga tgccctgatt
      301 aaagcctgcg acgataacaa agttcagtta tttgtcgtca aacaaaatcg gctaaatact
      361 acttcccaac tacttaaaag agccgttgac aaaggccgtt ttggaaaaat ttatatggcg
      421 cagacgaatg ttttctggca aagaccgcag gaatattatt cgcaggcaaa atggcgcggc
      481 acttgggaat ttgacggcgg ctcatttatg aatcaagcca gtcattatgt tgatgcgtta
      541 ctctggctgt taggcccagc ggatcacgtt atggctgaaa ccgctactat ggcacgcaaa
      601 atcgagaccg aagataccgg ctcggctata attaaattcc gcaatggcgc aataggaacg
      661 atcaacgtca cgatgctgac ttatcccaag aattttgaag gttctataac aattcttggt
      721 gaaaaaggca ccgctaaact tggcggcgta gccatcaaca aaatcgagaa atgggaattt
      781 gaagattatg acgacgacga tcgtttggtt cacgaagcca attacaatcc accaaatatt
      841 tacggcttag gacatttacc ttattataaa aatgtgattg ccgttttgaa tggagatgag
      901 gaagccaaca ccgacggacg cagtggtcgc aagagtctcg aactgattat cgcgatctat
      961 aaatcagccc aaactggcca gaaagttgcc ctaccgctga agctataaga ggttaaggtc
     1021 gaggtagagg tagaggagaa g
//
LOCUS       DAOOMU010000247         1031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_200354_length_1031_cov_17.943648, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000247 DAOOMU010000000
VERSION     DAOOMU010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200354_length_1031_cov_17.943648"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PLI55_10525"
     CDS             complement(<1..308)
                     /locus_tag="PLI55_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HPB01307.1"
                     /translation="MLTKVLAYTSLYIIAIFVNGIIVKLLLKPFLPEDMPAGTPNAGR
                     MIGYLERALILTFLIIEQPTLIGFVLTIKAIFRYGDIQGDCNTKMKISEYFIIGTLLS
                     V"
     gene            complement(395..1003)
                     /locus_tag="PLI55_10530"
     CDS             complement(395..1003)
                     /locus_tag="PLI55_10530"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HPB01308.1"
                     /translation="MSKYLVITADMINSRDVSTEQIATIPEKLKQIDADFHTLIPSML
                     FAGDEIQLLFPYDQNHHPYRLLMDLNRHLAPFHFRFGFGIGAIDQPINPIIAHNKGQA
                     FLQARKALAIAKKDQLIAWLSTEEIVQYSEINACFLLLTALTRKWSDKHWRRFYLYWD
                     NRSIHKVAEIENVSPESINKLLNNAGIRLIIRSLELITELHE"
BASE COUNT          322 a          175 c          210 g          324 t
ORIGIN      
        1 acgctcagga gggttcctat gataaaatat tcagaaattt tcatctttgt attgcaatcg
       61 ccctgaatat caccataacg gaaaatggct ttaattgtaa gaacaaatcc tatcaacgtt
      121 ggttgctcaa taatcaaaaa tgtcaggatc aaagcccgtt cgagataacc gatcatgcga
      181 ccggcatttg gcgttccggc cggcatatct tcaggtagaa atggcttaag taataatttg
      241 acgataattc catttacaaa aatagctatg atatataagg aagtgtaagc aagaaccttc
      301 gtcagcattt taatttttga ttattggcat tttatcgttt ttgaattggc tatagaggtt
      361 gacaatttta tttatcaacg aatatagttg acatttattc atgcaactca gtaattagtt
      421 ccagactccg aataattaat cggataccgg cattgttcag cagtttatta atggattcag
      481 gagatacatt ttcgatttcg gcgactttgt gaatgctgcg attatcccaa tacagataga
      541 accgacgcca atgtttatca gaccatttcc tggtcaaggc ggtcaagagt aaaaagcagg
      601 cgtttatttc ggaatattgg acaatttctt cagtggatag ccaggcgata agctggtctt
      661 ttttggcaat ggcaagtgct tttcgggctt gtaagaatgc ttgtcctttg ttatgagcga
      721 taattggatt gattggttga tcaatagcgc ctattccaaa accgaatcga aaatgaaaag
      781 gagccagatg gcgatttaaa tccattagta agcgataggg gtgatggttt tgatcataag
      841 gaaaaagcaa ttgaatttca tcgccagcaa ataacattga aggaatcagg gtgtgaaaat
      901 cagcgtctat ttgctttaat ttttctggaa ttgtcgcaat ttgttccgta gaaacatctc
      961 gactattgat catatccgca gtaatgacaa gatacttact catagaaaat taatcatagt
     1021 ttattaaccg c
//
LOCUS       DAOOMU010000248         1016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Fidelibacterota bacterium isolate AMR_MDS_2667
            NODE_205194_length_1016_cov_20.057232, whole genome shotgun
            sequence.
ACCESSION   DAOOMU010000248 DAOOMU010000000
VERSION     DAOOMU010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864742
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Fidelibacterota bacterium (wastewater metagenome)
  ORGANISM  Fidelibacterota bacterium
            Bacteria; Pseudomonadati; Fidelibacterota.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 11:16:35
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,108
            CDSs (total)                      :: 2,066
            Genes (coding)                    :: 2,057
            CDSs (with protein)               :: 2,057
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 9
            CDSs (without protein)            :: 9
            Pseudo Genes (ambiguous residues) :: 0 of 9
            Pseudo Genes (frameshifted)       :: 0 of 9
            Pseudo Genes (incomplete)         :: 9 of 9
            Pseudo Genes (internal stop)      :: 0 of 9
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Fidelibacterota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_205194_length_1016_cov_20.057232"
                     /isolate="AMR_MDS_2667"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026760"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLI55_10535"
     CDS             complement(<1..301)
                     /locus_tag="PLI55_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPB01309.1"
                     /translation="MSRAREAFAQITENKTYPIPINGVLDLHTFSPKEIKDLIPEYLE
                     ACRAKDLLDVRIIHGKGIGVLRETVQAILRKLPYVEKFQLADESGGGWGATIVRLK"
     gene            complement(356..886)
                     /locus_tag="PLI55_10540"
                     /pseudo
     CDS             complement(356..886)
                     /locus_tag="PLI55_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
BASE COUNT          270 a          239 c          228 g          279 t
ORIGIN      
        1 cttttaagcg cacaatcgtc gcaccccagc cgccaccgga ttcatcggct aactgaaatt
       61 tttcaacgta aggcaatttg cgtaaaatag cctgaacagt ctcccgcaat acgccaatgc
      121 ctttgccgtg aatgatacga acgtcgagca agtctttagc acggcacgcc tcaagatatt
      181 cgggaattaa gtccttgatt tctttaggtg aaaaggtatg tagatcgaga acgccgttga
      241 tcggaatagg atatgtttta ttctctgtaa tttgagcaaa cgcctcgcgc gctcttgaga
      301 taaaatgccg cacgagttta gcgtccttcc atccagcggg attctccacc ccagtatgag
      361 tatccactcc ggccggtttt acggttaaaa ttgcctcacg aatatttatg ggatttaagc
      421 ccccagctaa aataaccggt ttaggtgaat atgcgacgat tttccggctg atttcccaat
      481 catgggtctg gccggtggcg ccggtcgcgc ccgtcttagg atcgtatgta tctgtgataa
      541 aagcttcaac cagactggcg aattgatcaa tagtatagcg caattcatcc atatttccag
      601 aacgcacgat taaacttttg atgattctca gatcgggatt tatgcgacgt aacttttgaa
      661 cttcgttggg taagatgaaa ccgtgcagtt gcaaatattt tactttgagc aaacgactca
      721 ggctcgcgat ttcttgcgca ttatcgagat aagtgattaa gacacttcta tcgccgcagg
      781 cgagtttggc gatgatttcg gcggcttcga cttcggttag atccggttgg tgataagcca
      841 gccgcaatgg gaaaccaagg caatccgcac cgctctcgag tagcattcga gcttcatcta
      901 tacttcgaat accggcgatc tggatgatgt tttccataat tgaaaattat cgttccatac
      961 caactaaaac aagttgcaaa aaccaatctg ccgattagga cggcacgcta aattat
//
